LOCUS       DAOPNP010000001        92575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_290_length_92575_cov_9.954777, whole genome shotgun sequence.
ACCESSION   DAOPNP010000001 DAOPNP010000000
VERSION     DAOPNP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 92575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 92575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92575
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_92575_cov_9.954777"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1103
                     /locus_tag="PKI64_00005"
     CDS             81..1103
                     /locus_tag="PKI64_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOD99417.1"
                     /translation="MGDKVHSICILDASGTVRECAEVSNTRAGIYKFFAKMEPSLIAL
                     ETGTHSSWVSRILEELGHEVLVGHARKLRMIWNNPQKNDMRDAEMLARIARFDRKLLH
                     PIQPRSMKAQCDLAAIKARDQLVKVRSSLIAHVRGLVKSVGARLPTCSPECFAKRAAE
                     AVPEELALALEPVIVSIAELTVRIRHCDQLVQKLADEEYPATKRLQEIPGVGPITSLA
                     FVLTLEDPKRFARSRDVGPFLGMTPRRNQSGQVDQQLPITKAGNAYMRRLLVQCAQYI
                     LGHYGPDCDLRRFGLKLAERGGKNAKRRATVAVARKLAVLLHSLWKTGEEYEPNRCRR
                     QNRKTA"
     gene            complement(1440..>2570)
                     /locus_tag="PKI64_00010"
     CDS             complement(1440..>2570)
                     /locus_tag="PKI64_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOD99418.1"
                     /translation="VSGEGQLPAGLTLEDDGFLHGIPTQVEALFFTVRATNQAGASNR
                     VYNITINAPADEPEFITDSPLPDGVVGQPYLCDIEVANYPNFIGLYSGAFPEGLGITL
                     EGQITGTPVKVETANFILYATNMVGTASKSYSLHISGPPIFVTDSPLPFGSLGEPYSQ
                     QIEAIGDPLFLVFSGALPGGLDLATNGWLTGTPTTTGDFNFTVRATNDFGYTNKDYSI
                     NIGAGPVFSTTNPLPNGRESSAYSVWIVASGDPDYTLLSGTLPGGLTLSLAGELSGIP
                     TNAGNYNFTILASNQYGIADREFDLAILGATPPRITAIRATNGNLVLTWTNYNDVGGV
                     TVWRSHSLTSEPVPWTNLGVQTSPWTNPAPTNTTYFHLRLAP"
     assembly_gap    2571..2670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2671..3689)
                     /locus_tag="PKI64_00015"
     CDS             complement(<2671..3689)
                     /locus_tag="PKI64_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99419.1"
                     /translation="MKSLPSLRAWFVAILLGGLWFAGTATAATHPLGWGNNSAFKAFP
                     VPTNVMSGASAIAAGYDHSLAVKDGRVWAWGTNDFGQTNVPVAAQSGVTKVSAGSSFS
                     MALKSSGDIVVWGSPVLSSNIPANAVSGVSAISAGEWHALVLKNGSVIAWGSNSYGQC
                     NVPVSLTNGVTAIAAGGFYSMALKDGGVQVFGISATNQLAWGIRDIPTEALSGVSAIA
                     AGRWHALALKNGKVIAWGAPHDDATDVPLAAQSDVTAIAAGDLFSYALKTDGSIVHWG
                     AYFDGQDQIPLAAATGVTAIAAGVSHGLALCPVMPPRFLATTLPDAYQNKPYTNQLLV
                     AADPAA"
     gene            3813..>4459
                     /locus_tag="PKI64_00020"
     CDS             3813..>4459
                     /locus_tag="PKI64_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD99420.1"
                     /translation="MRKIKLVILDAHATNPGDLSWEPIEALADCTIYPRTPPAEVLAR
                     VGDAEAVMLNKGVLSAETIRRAPNLRYVGILATGWNTIDLPAAKERGLTVSNVPGYST
                     DSVAQHTFALLLELTNQVGLHSVDARSGGWARNPDYCYRLSPQIELAGLTLGIIGFGQ
                     IGQAVARIGQAFGMTVLANRRDQTQRLPPGVRYASVEEIFAQSDVVSLHCPLTPET"
     assembly_gap    4460..4559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4560..4849)
                     /gene="gcvH"
                     /locus_tag="PKI64_00025"
     CDS             complement(<4560..4849)
                     /gene="gcvH"
                     /locus_tag="PKI64_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOD99421.1"
                     /translation="MSSGELRYTAAHCWVREEGSVVVVGITDYAQEQMSELTFVELPE
                     VGEEVAADDEVAVVESVKAANDVYAPLNGTITEVNEALLEQPELVNSDPFGKG"
     gene            complement(4984..6255)
                     /locus_tag="PKI64_00030"
     CDS             complement(4984..6255)
                     /locus_tag="PKI64_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD99422.1"
                     /translation="MARYKFTALDPKGKEVHGEIESDSQSQAVARIREKQLFPTKVEE
                     LAGGGAVAPRRSGGKGKSALQMELRLPKFLQGVKSKQLTTFTRQMSTLVSAGLPLMRA
                     LRVLQRQEKNPALRDAVVQMAESIESGSTFAEALAAHPKIFDRLFVNMVKAGELGGVL
                     EVVLARLAEFQEKAEKIKGKVKSAMTYPIVVLVMALSILTFLMTYIVPKFADIFADLM
                     GGKGMPMLTQMVMSASSTMVQRFPVVLGVIVAIVVIVKLLAKTTQGRYLIDKFKLHAP
                     VFGTLISKNSISRFTRTLGTLMSAGVPVLQALNIVKETVGNEVISRAVTLIHDAVKEG
                     ENMAPPIAASKVFPPMVVSMVEVGEETGALPDMLMKIADSYDDDVDNAVAAMTSIIEP
                     LLIIFLAVVVGTIVIALFMPLVSIIGGLSGA"
     gene            complement(6307..8013)
                     /locus_tag="PKI64_00035"
     CDS             complement(6307..8013)
                     /locus_tag="PKI64_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD99423.1"
                     /translation="MTEYSDMFLEELVRTGLLTDTQAAEIMDEHARSGKPPKDIVQEM
                     GLVTEDQALDIIAQLMGAERVDLEHMSVAVEIIQTVPGSVARMYSIMPVAADGNSITL
                     AVSNLVTPETTDEIRFVLSKDIQFAVTEPAAITNALSRYYGSEDESMKDMLSSLESEL
                     EGAGDLLESGARGDDIASMEAAANSRPVVRFVNLVLYQAVQDRASDIHFEPFEDEFKI
                     RYRVDGALYEMAPPPRHLALPVTSRLKVISGLNIAERRIPQDGRIQLPVAGRMIDFRV
                     STLPTQFGESVVLRVLDRSVVSLDLENIGMPDDIYQYFCEDIDKPNGIMVVTGPTGSG
                     KTTTLYSALRRLNTIENKLLTAEEPVEYDIDGIIQVPIHEAIGVTFSRVLRAFLRQDP
                     DIIMVGEIRDIDTSEIAIQASLTGHLVFSTLHTNDAAGAITRLIDMGVEPFLIASTLE
                     AVLGQRLVRTICNNCKTAYQPTPEILQQLGMTEKQVGGRPFYYGAGCSYCNQTGYKGR
                     RGIYEYLRVKDPIRDLINKRKPTLVIRDKAVELGMRTLREDGIRCILDGYTTVEEVLK
                     YT"
     gene            complement(8027..9115)
                     /locus_tag="PKI64_00040"
     CDS             complement(8027..9115)
                     /locus_tag="PKI64_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOD99424.1"
                     /translation="MSTLRAADIQMDDLLELAVAEGASDLHLAVGLPPVLRIHGSLAP
                     LDAEPLRPEDTERLVRSFAPAHHLEALERDLGSDFGFPFRDLARFRVSIFRQKGHVGI
                     VLRQIPTKMLTLEEIGLPAQLKELLFKPRGLILVTGPTGSGKTTTLASMLNIINQERD
                     CHIITVEDPIEYYHTHQRSVVTQREIGVDVPTFKEALRRALRQDPDVILVGEMRDLET
                     MEAAITAAETGHLVFATLHTTGAARTVDRIVDAFPTNQQEQIRAQLSTSIVAVISQIL
                     MPRKDQPGRIAAFEIMIATPSIRSLIRDNKTFRITSDLQTGAKWGMQTLDSHLIELWR
                     GGKIAYEELVTKAQDTEEVLQQIRTNQK"
     gene            complement(9115..10824)
                     /gene="gspE"
                     /locus_tag="PKI64_00045"
     CDS             complement(9115..10824)
                     /gene="gspE"
                     /locus_tag="PKI64_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOD99425.1"
                     /translation="MTSNDDYILEILENVGLITREQAVTARQLATQDDEGVVDVLARE
                     GVVDKLDVLKALAAQFGMETITLTGLDIPREVLDMVPGDVAQRYKVVPVFKNENVLTV
                     ALGDPLDVDTLDGLRYVLKTNVEGVVAPPEEIEKAIANYYGRATGAVESMLTEITEGT
                     LALPDEVKNQLVADENITEADAPIIKLVSLIIMEAFRNRASDIHLEPMPKKFRVRYRI
                     DGVLHEVDSPPKRLQSAIISRVKIMANMSIAEKRVPQDGRIQINVMGRDLDLRVSSIP
                     TNHGESIVMRILDKAGIALGLPQLGFFSDDQQVFERLIGLSDGIILVTGPTGSGKTTT
                     LYACLNAINKPDRKIITVEDPVEYQMSGINQVQVRTDIGMTFAAALRSILRQAPNIIM
                     IGEIRDMETAEIAVNASLTGHLVFSTLHTNDAPSAVTRLIDIGVKPFLVASSIRAIMA
                     QRLVRRICDKCKEPYTPEEAELRLLGPAAQQLAAAQLFHGKGCADCAFTGYRGRLGIY
                     EIFQIDDQVRNLIFDQVAAVELRTKARELGMRTLREDGLRKVVAGLTTVQEVLRVTMG
                     DSD"
     gene            11091..12275
                     /locus_tag="PKI64_00050"
     CDS             11091..12275
                     /locus_tag="PKI64_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD99426.1"
                     /translation="MRRKPIALLSLGHAVTDLVQGGLTMLLAFLQPTLGLGQLQVGMV
                     MLAFNVSSSVIQPAFGVFSDRFHAAWLIPSGCLLAGVGMAAAGYMPDYPLLMLAAFIS
                     GIGVAAYHPEASKFARQASGKRKASGMSLFSVGGNFGFALGPILATGFLALAGRNGTM
                     GFLLLNGLMALVLWRKLPAITATPAHVAGTQQPPRPKTRFTWAVVYPVVILVLLIIMR
                     TWTHFGIVTFLPQYYIHHLDYSKEHAAALTSLFLFAGAWGTLAGGPAADRWGLKPLIV
                     ASLVVMIPLLYLFPRTSGVPGLIVIALAGFALIATFATTVVLGQELLPNNVGLASGLT
                     LGFGVGTGGIGATLLGAVADRWGLPMIFNWMIGFTLVGLLLALMLPGSAALAARREHA
                     RY"
     gene            complement(12272..12859)
                     /gene="lexA"
                     /locus_tag="PKI64_00055"
     CDS             complement(12272..12859)
                     /gene="lexA"
                     /locus_tag="PKI64_00055"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOD99427.1"
                     /translation="MPRDIRLAEKIRQLRHFWRQERRAPSYAEMMELFGYKSRNAVFG
                     LLLKLEEAGCLTKDDHGRVALLPPITGTVRVLGSVAAGFPTQEEEQEADAITLDEYLV
                     KNPDNTFMLTVRGDSMINAGILPGDIVLVEKGPRPNQNDIVVARVDDEWTLKYYVRDA
                     AGVRLEPANPKYRFIRPQRSLEIGGVVRAVIRKYN"
     gene            13021..14826
                     /locus_tag="PKI64_00060"
     CDS             13021..14826
                     /locus_tag="PKI64_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99428.1"
                     /translation="MKKWSSLIAIVLLLGLLVMPGWASDTPSVLGLVEIRGLDALAGV
                     VFDLTKSVGNPIPKEMTSMILYSGLGSMPGMGITPDGNVRVVWFESASPAGSFVLYLP
                     VDNDGADYFAYLDQNGWENQGRLSDKIEHYSPPEDAGVLWENVYFIRHGATLVAADNP
                     GDARKGESLLNALPPILPVEGVLAIQVRPAALTDTYGPLVREQMDVMVNDPDAPAQME
                     PFAKEYFDAYLQVAAQVDEVVLGVGVANDKLNLHYRAAPIAGTTLARWMDTIRAPGAA
                     SAVVALPGALIVSAVNMGDFNMIAGPYLRFMEKALKSMPQEIPEEFITRYMALEKAGY
                     EQLVGDISMALLPPKADLPLRFVEYANLKDVAATRGLFVEGVNLFNDGIAAMLASVPE
                     AKESGVNLTATLSTPRTYRDIPVDQMRIAIELPEEAQAEWPAFLPRTFTADVAWLADG
                     AVFSVGDPALIEPLVDRALDGATDGVTSLPAWKAAFPNPETPIVEASHIALFDTIRSF
                     VQWLDAATGENHAPAIPAGPGNVESASYLQDGYMSQLRINLADLGAIGAKISEAREKA
                     MQQRQLYLGGDTNDWYDMDDTDEDEDSSEETPATE"
     gene            14830..15381
                     /locus_tag="PKI64_00065"
     CDS             14830..15381
                     /locus_tag="PKI64_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOD99429.1"
                     /translation="MRPTCLSIFWSFLRIGAGAFGGGMAALPVFEAELVNRRRWLSAP
                     DTAELFAIAQSGIGVIIVNFAVLAGLRLVGKRGAFVAVVAVVLPAFFVILALAMLVAG
                     NWENRWLSAALVGLRPAVVAIIVGAAWRLGRANLRTFWPILAAAACAVLLFQRVCSPV
                     VLILLGIAVGLILHWLRRPRPAP"
     gene            15378..15923
                     /locus_tag="PKI64_00070"
     CDS             15378..15923
                     /locus_tag="PKI64_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOD99430.1"
                     /translation="MMIYLQLFATFFEISLFSIGGGYNMIPLMQHAVTANGWLSAERF
                     LDILAVSEITPGPIAVNLATFAGYHTAGFWGAVAATVAVCLPGSVILLVLGSLAFRLR
                     DRPAFRAAMRGLMPALVGLLTATALRLATALIPAGAHWPSLQASVVFAVALTLFLWKN
                     LHPAVLMLGAAATGVAWSLVA"
     gene            complement(15953..16363)
                     /locus_tag="PKI64_00075"
     CDS             complement(15953..16363)
                     /locus_tag="PKI64_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99431.1"
                     /translation="MKGREPAKVEIQCPACGREAWLVRKPKYDGFTKVGETLTCALCA
                     HEFASESEVPFRGSRQPQVFTAADRPRPVQVFREDEKGRMCRYCAEYVVNPFIQRCGL
                     HKCEVQATDTCPHFRPKPPPKEEAENETPGPFRL"
     gene            complement(16365..17411)
                     /locus_tag="PKI64_00080"
     CDS             complement(16365..17411)
                     /locus_tag="PKI64_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD99432.1"
                     /translation="MAIGKILVTGGSGFLGINLIRHLRAKGETDIRSLDIVPFDYPEK
                     DSVELHTGDIRDVAAVARAMDGVSMVIHTAAALPLYSERDIMTTDVDGTRNLLAAAKQ
                     AGVTRFVMISSTAVYGIPDHHPLLETDKLTGVGPYGRAKIAAEEECIAFREQGMCVPI
                     IRPKSFIGPERLGVFALFYDWAYTGHGFPMIGNGRNRYQLLDVEDLCEAIWLTMTLPA
                     AVVNDTFNIGAKDFTTMREDYQAVLDDAGHGKKIRGFPAAPMIWTLRFLELLKLSPLY
                     KWVYETASEDSFVSIEKAERVLGFRPKYSNKEALVRNYRWYVANLDAFRNASGVSHRV
                     PWKQGILRLAKWFF"
     gene            complement(17435..18406)
                     /locus_tag="PKI64_00085"
     CDS             complement(17435..18406)
                     /locus_tag="PKI64_00085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD99433.1"
                     /translation="MRILVIKLSSLGDIAHALPAVRALQARTGATVDWVTQPEYGNLV
                     AGLRGVERVILFPRRNFARGFWAFWRELRWERYDAIVDLQGLLKSALVARLARGRRRY
                     GPAWAREGAARFYHEQPAKTAGPRGHATAEMLAVVNLLAPGTEVPELQLDFAENPGDG
                     SPGPHVAFAPFSRWATKNWPVEKFAELGQRMIREMGCRIYVVGGAGDTEAGESLARQI
                     GERCRNLCGKTDLPGLCGLLKGMDLLVGVDSGPLHWADALGVPLVGIYGATDPARTGP
                     WRQRDGVVVKEGLDCRPCHARTCRRGDLACLMDLDVDTVLRATLAKL"
     gene            complement(18408..19199)
                     /locus_tag="PKI64_00090"
     CDS             complement(18408..19199)
                     /locus_tag="PKI64_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOD99434.1"
                     /translation="MKILVVGDIVGGPGRMAMARVATRYKAEKRADFVLANAENSAAG
                     RGITAALAEELFAGGADALTMGDHVWDQKEAAAYFDREPRIVRALNLPPGCPGHGVST
                     VPSPAGPVTIVQLMGRTFMNTVADCPFRAVDAWLKEHGAAHKIIIVDMHAEATSEKVA
                     MGKFLDGRVTAVLGTHTHIQTADEAVTAKGTAYMTDLGMTGPCDSVIGRTTEAILTRF
                     LTGMPTKFEIASGDVRLHGALLTVDPETGKALKIKRVEEILSRPG"
     gene            complement(19222..20784)
                     /gene="rny"
                     /locus_tag="PKI64_00095"
     CDS             complement(19222..20784)
                     /gene="rny"
                     /locus_tag="PKI64_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOD99435.1"
                     /translation="MSVFWEWFWPVLTAAGFTLIGVGLHMLVVRLRSRSAQRGAAEIL
                     QAAQRDAEVIRKEAQLAARDELLRKREQAESDLQQRQLVLSAAADRLSRQDERLEREE
                     QRLEERAQALDVRAQQLEAARATLTAEQEKLAATMATAQQRLETAAHLTADQARAELR
                     AGLQEEIAAETADWLRKAQAAARAEAAHDARRLLMDVLPRVAVDHVSEITTVAVRLPD
                     ESMKGRIIGREGRNIRALELATGVSVIIDDSPEVVVLSSFDPIRREVAKVALERLVAD
                     GRINPALIEEMVDKVAAEVDGLILQAGQDALTELNLAPAAGEVVRALGRLKFRTSFAQ
                     NVLQHARETAHVAGLLAAELGLNADIAKRAGLFHDLGKAATQEAGGPHAEIGADLLQH
                     AGESAEVVAAVRTHHVETAPANVYAALVAVADAVTAARPGARSDTTEQFLQRLKNLEE
                     IAAAEPGVVRSLAMQAGRELRIFVEPTAVDDAAAIQLARRIAQQIETRVDSPGPIKVT
                     VIRELRCVEYVR"
     gene            complement(20786..21349)
                     /locus_tag="PKI64_00100"
     CDS             complement(20786..21349)
                     /locus_tag="PKI64_00100"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOD99436.1"
                     /translation="MKTKLRAQFKNRRVAPAASAAIQKMLLAQDWWVAGFQVGLYRAV
                     ATEPATDLLLADLLARGAQVAVPRREKNGYVWSWVDATTRWVAGAHGIMEPALRVPAA
                     AAELRIIVVPGVAFDRAGGRLGHGTGHYDRLLAGSDALRVGLCDEARLVTQVPMDAHD
                     ARMDLIVTEKQVRFMAGAEEKLARLTG"
     gene            complement(21346..21738)
                     /locus_tag="PKI64_00105"
     CDS             complement(21346..21738)
                     /locus_tag="PKI64_00105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOD99437.1"
                     /translation="MKHVIQLTVRPYECDSYGHVNHAVYVNYLEHARMQFLHAVGFDY
                     KGLIAAGFFTVIARLELAYRQPAYADDELTIETEAGEPRRVGGAFQQVIRRGEDVIAE
                     ATVHWCVVNAKGRPTRPPAGFDLRGLLK"
     gene            complement(21811..22551)
                     /locus_tag="PKI64_00110"
     CDS             complement(21811..22551)
                     /locus_tag="PKI64_00110"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOD99438.1"
                     /translation="MKILAVNIGNSRTAAGWYSNGKIRRAARTESVSPVILETVANGE
                     IPDGICLGSVVPKLNTTWTRLVRQTFPRVPVLWLDTDLDYGINLDLKRPDQTGHDRLA
                     NAVAGAELCGTPCIVCDFGTATTFNLVLARRGFVGGVIAPGFGMWFTAMGQRAAQLPK
                     LKPGGIKLPTGRNTEEAMRLSARWGFRGMVTEILWQLSKACGKKEPIVCATGGYARKV
                     LATAGLKIPVYPDLTLHGLARIYELNRG"
     gene            complement(22548..23153)
                     /locus_tag="PKI64_00115"
     CDS             complement(22548..23153)
                     /locus_tag="PKI64_00115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOD99439.1"
                     /translation="MPWGYPRRWLATTESTNDVARDWALAGAEAGALVVAAQQSRGRG
                     RRARPWQSLPAAGVYASFILRPDWPAAQAPGLAILGAMAAHDALHAAGVPELRVKWPN
                     DVQARGQKICGVLVEPRLGAGRIEFAVVGIGINVAQARGDFPAELQGRVTSCRLQGCQ
                     ISVDEMLELLVESLRRVVQAPLADVRARWLAAGAREDEPEL"
     gene            complement(23161..24024)
                     /gene="nadC"
                     /locus_tag="PKI64_00120"
     CDS             complement(23161..24024)
                     /gene="nadC"
                     /locus_tag="PKI64_00120"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOD99440.1"
                     /translation="MNPDNTLMQDAEVRELIARALAEDVGSGDATTLALVGAGETARA
                     RLVARHEVVVAGMEIAAAVFRQADPALQVEILCPDGECVAAGTALLTVAGPARALLTA
                     ERTALNFAQRMTGIATATECLVRIVADYGTLILDTRKTVPGLRKLDKYAVQCGGGTNH
                     RQGLHDAVLIKDNHVAFWRRTQQGSLADCVRAARRAYPNLKIEIEVDSLEQLREALPG
                     QPDWVLLDNMTPAQVAEAVELCGGACKTEASGGITKENVREYARAGVTAISVGALTHS
                     SRAADLGLDFE"
     gene            complement(24047..26122)
                     /gene="uvrB"
                     /locus_tag="PKI64_00125"
     CDS             complement(24047..26122)
                     /gene="uvrB"
                     /locus_tag="PKI64_00125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOD99441.1"
                     /translation="MVAGFELVADFQPTGDQPEAIAALVAGFREGAHYRTLEGVTGSG
                     KTFTMANVIAQLNRPTLVISHNKTLAAQLYAELKEFFPRNAVEYFVSYYDYYQPEAYI
                     PQTDTYIEKDASINEEIERLRLAATDALINREDVVIVASVSCIYGLGSPEDYRGMLVT
                     LRAGEEFAREDIMRKLVDVQYTRNDISTLPGTFRARGDTLDIFPGYSQDGVRVEFFGD
                     RIESLRRIDPLTGKTLQHFEYTTISPAKHFVMPAEKIAPAVTRVLAEMEARVAELEAQ
                     NKLLEAQRLRMRTTFDMEMLREVGYCSGIENYSGPLSGRPRGSRPYTLMDFFPRDFLT
                     VIDESHVTLPQIRGMFNGDQARKRTLVEHGFRLPSALDNRPMSYDEFMGLTNQVLFTS
                     ATPGPTEFQLAGTPVPQIIRPTGILDPLVEVRPLRNQVDDLIEEVRQCAERKDRVLVT
                     TLTKKTAEDLSVYLQNIGIRCKYLHSDITALDRVDILRGLRKADFDCLIGINLLREGL
                     DLPEVALVAVLDADKEGFLRSETSLIQTAGRAARHLQGRVILYADQVTDSMQRMVDLT
                     SERRRQQEEYNRAHGITPRKIEKAISESLMQAEKAREAEEFAVHEAGADYDVVRAIAE
                     MEREMFEAAEAMEFERAALLRDQIYELKKTENPFKGTVQIGKRKAIVYHPGKRLPGKR
                     PSSTRRRKS"
     gene            complement(26138..26665)
                     /locus_tag="PKI64_00130"
     CDS             complement(26138..26665)
                     /locus_tag="PKI64_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD99442.1"
                     /translation="MSPAFHDLATTRRSVRAYKAEPVPEALLQTVLEAGRLAPSACNK
                     QPWRFIVVRNETTRRALGAAYAREWFWKAPVIIVVCVVPGEAWTRSYDGKNYALVDGA
                     LAMDHLMLAAAEAGLGTCWVGSFDPAAVRTTLGLPDGVEVVGMTPLGFPDVEPNPRPR
                     SRRPLGETVMKERWG"
     gene            complement(26669..27619)
                     /locus_tag="PKI64_00135"
     CDS             complement(26669..27619)
                     /locus_tag="PKI64_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99443.1"
                     /translation="MTWTTRCWAALLALAMAAAPVCGAPAIVHDPIKVAVKGQPLGVR
                     ATVRDATARVEGVSLFYAASRGMTPFRVPLTSSGAGTWYGTIPGHMIGPGTQLFYYIQ
                     AENADGESRETEWQTVKVVESGVAPEAIPAASTVAQQAQQQMAGPATAQPPAAPPKSG
                     KSKYLLPAAIIVGGAAAVGGALAIASYNSGSSSGEEPNPAVAGNYGGSYDICFHAAAT
                     TNNPTPAPVCDAGLVNIYVTNGTAQIVGLWGAEVLSGPVNGNVFTIVKETSATAKFPS
                     AHLIAAGEFAGHSATVRIDGYSTDPLQPGNFGGRLQTTKR"
     gene            complement(27746..28753)
                     /locus_tag="PKI64_00140"
     CDS             complement(27746..28753)
                     /locus_tag="PKI64_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOD99444.1"
                     /translation="MRKEAQILLFPELGDGLEAPKPINVASIPQRSPFRYPGGKTWFV
                     PTFRSWISSIFPKPQYLVEPFVGGGIISLTALFENLVERAVMVEIDDDIAAVWRAVVN
                     GEADWLARRIMEFDLTKDSVVREISRTPRSSKEKAFQTILKNRTYHGGILAAGSGFLK
                     YGENGKGIKSRWYPATLARRLTNMNLIARRMEFRQEDGLAVIQRHAADQRAAFFIDPP
                     YTAGGKKAGKRLYRHCELDHHRLFSICETIAGEFLMTYDNAEEVKIMARAHGFQMRLI
                     PMTNTHNATMSELVIGKDLAWLDQYPAVGEPKVSYRVKKAANKAKTSARRTRKPEPEQ
                     A"
     gene            complement(28753..29694)
                     /locus_tag="PKI64_00145"
     CDS             complement(28753..29694)
                     /locus_tag="PKI64_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HOD99445.1"
                     /translation="MGTEIARRTQKAKERDAVIEMKQKHQPLAEVFGHLITDQSASAN
                     RYRLRRLCPFNNSVPNCTKDKAKNPLGVCSVLHDGQPAITCPIRFREDWLITDDAASF
                     FFPDDVAWSSLMEVRLNDAYGKSAGNIDMVLVSYDSEGKVLDFGALEVQAVYISGNVR
                     APFEYYVKDPKRRGEMDWSDQPNYPRPDFLSSSRKRLAPQLLFKGGILNSWGKKCAVA
                     LNKSFFDTLPRLTTTAKSKADIAWFIYDLQLSEHSGQPRYRLTKVDEVFTEFTPALRS
                     ITTPVPGKVESFVKQLQERLDEKLETPPANQTIERPF"
     gene            complement(29716..31143)
                     /locus_tag="PKI64_00150"
     CDS             complement(29716..31143)
                     /locus_tag="PKI64_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99446.1"
                     /translation="MKKLIPLLLLLPMAAWATGPQVTIYNDGFATVKETRTLQLTKGV
                     STVRVTDMSRQLEPDSVMLRELGKKPFGVRILEQSFVNDPLTEGLLLYQLEGQNVRFE
                     EKRRDGTIKEHRGKVIRSGYIPGGRSEQPIIETDGAVRFSLPGQPVFEGLDPAAFLKP
                     SLVWQLAAERAGECPVEVAYVTEGMSWEATYNLVMPAEGGDTFDFSGWISLQNETGKD
                     FTDTTVKLIAGDVQKLDRGFDGRFGVDVVMEMAVADAMPEERSFDEFHLYTLPRPVQV
                     RNNELKQVEFLRASGVKGTRYYVYNPLVDARYWRRLNSDPDYGQTAIKKVGVRIEIEN
                     RENNQLGVPLPKGRMRLYRTDVDGRREFIGENELDHLPKNEKLKLEMGSAFDLVGERK
                     QTDFSVDTTAQRMSETIEIKLRNRKEKEAVEIRVIEPLYRAANWKISAASAEWTRFDS
                     HTIEFRVPVLADGETVLTYTVNYSW"
     gene            complement(31167..32591)
                     /locus_tag="PKI64_00155"
     CDS             complement(31167..32591)
                     /locus_tag="PKI64_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOD99447.1"
                     /translation="MKKLIALLLFPAAAWATGPQVTIYNDGFATVKETRNLQLTEGVS
                     TVRVTDMSRQLEPDSVMLRELGKKPFGVRILEQSFVNDPLTEGLMLYQMEGQNVRFEE
                     KRRDGTIKEHLGKVIRSGYIPGGGSEQPIIETDGAVRFSLPGQPVFEGLDPTAFLKPS
                     LVWELAAERAGECPVEVAYVTAGMSWEATYNLVMPAEGGDTFDFSGWISLRNSTGKDF
                     ADTTVKLIAGDVQKVTRRPEYRQPLVLMGARPAPAAMPEERAFDEFHLYTLPRPVQVR
                     NNELKQVEFLRASGVKGTRYYVYNPLVGYGYSRSANTDADYGQTASKKVGVRIEIENR
                     EDNQLGVPLPKGRMRLYRTDVDGRREFTGENQLDHLPKNEKLKLEMGSAFDLVGERKQ
                     TDFSVDTTAKRMSETIEIKLRNRKEKDAVEIRVIEPLYRSANWKISAASAEWTRFDSH
                     TIEFRVPVPADGETVLTYTVNYSW"
     gene            32760..33650
                     /locus_tag="PKI64_00160"
     CDS             32760..33650
                     /locus_tag="PKI64_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99448.1"
                     /translation="MADLIKRLGVALLAGALAAGGSACGKKNATGGDVATLISEAWTK
                     YRLSEFQDAQLTFEAARASAQPGSDAWAMATYGLATTWDLRRPGEDPKLATKLYQEIL
                     TNAPASPMIPWTELALARQKHLVPVGQEPDYAAVHKAYQHIMDAFPGHLAAKEAFLYQ
                     ESIELAKLEEASTRAAVKRLNQFVQSDSTEFTGPAWSLLAVAHTTLKNPAGRLHAEQK
                     SFELTEVDPADPFVEFAWAYWNLATIAEFEVGDFDLARHYYNKLLEAYPNDIRVYGCK
                     QALARMDALEAQIRAEEEGR"
     gene            33652..35409
                     /locus_tag="PKI64_00165"
     CDS             33652..35409
                     /locus_tag="PKI64_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD99449.1"
                     /translation="MRLIQKLLDFVRTYFALLVVFAVFLWSAWVIATYRAQQAPPGSI
                     VIRLGHWQLEASVREGLDEMARDYQKLHPNVVIIQDAIPEGTYGQWVSTQLMGGTAPD
                     IMQVGAMLPDNIWLSYYNRYFQPLSRYVNTPNPHNAGTDIADVPLRRTFKDGMKTAYV
                     AEMQEYMSVPLSQFGVRIFYNKDLLKKLTGLDVAPLDYREFLAVCQKIQSVTNPTSGQ
                     PYIPIAGSAYHFGHWDGMMFSPVTYGCFRRADFNRDGFVGNDETFVAFKTGQLDFLYP
                     PLACWLKMLREVTGYFQTGYTGLGRDEAVFLFAQQRAVFMTTGTWDARSLQEQAKGQF
                     EVGIMDFPIPARDDPVYGGVVEGRIYETPGAGFRFGISRTSKNFDVALDFLLFLASQR
                     GNEKLNRIIGWIPAIVGTELDPLLQAFEPHLEGIYGNINFNLGGNTAVTWGQIYSLYQ
                     VNQISFEDFATRFTEYYLQNGLKDFLEQQRDWRRGMQRNEQYLAGIRGRAILADEAAA
                     AATDPAAKAAAELDAASAWVRYRAITSSRQIWGELNHARQLDLITRDHLPADYVAPYE
                     YSPAVLTKIRERIRAEVAP"
     gene            35836..38247
                     /locus_tag="PKI64_00170"
     CDS             35836..38247
                     /locus_tag="PKI64_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOD99450.1"
                     /translation="MQPKEVRPNQLRHHWEIYFFIIPTLLLIALFQYYPASSGIFHSL
                     FRWNGSDISEFVGFENYTDLLKNLEFWNSFKLAFILGFWNVIKMIPALLVAVSIHRCT
                     SEKMQFFYRTLFVVPMVIPGLITVLIWRSFFFEATAGYLNQFLDWTGLMTLLQHGGNF
                     VAAKLADPGTTGLAKAVLESFDWLTAFKAGSAPAWLGDPKIIIIACVVWGFPWVGSFA
                     VLTHLAKLQNISKDIYEAADIDGANWWTRFTKIELPLLMGSIYLLLVFAIIDTIKDAG
                     MILALAGITGGPGGKATVPALFMLRKAFMDQQMGYACAVGIVLTVVVLLLQKICNMLL
                     QGEPEGGARPGKSAWFGILALYTFAEGFVFFRAHLVPHTATPLIFAAFVVTLLIKLGL
                     LVSVPVALRKYGRALRHGLLAVAAGLMAAAITGRADVTWLLFGYALVMMLSTSLFKAA
                     IGVAGAILIGYGATGGSLNMIISGAVFIILATPWGQLGARVDKAYLARCLRRPTPAVS
                     KGDLYVARSFARQQMPAYKAAAAVGSAWLRFAKHAFIWFVLAFAFLPLYLMLIVSLKD
                     NNQFYLNPTTLTQPLHWDNWSTAWAMVSPTVANSIFLSTTATFFIVIFGLAGAYFFAR
                     LKVPLSSFLWNALLLLMMMPMIANLVPLFILLRDMSLLNTLTALILVGASGGQAFAIF
                     VFRNFIADIPQDLFEAAEIDGANHFQQLKLVVIPLSGPIMGTVAVMQFLGQWNEFVMP
                     LIVMREQARLPVTVQLIRMAGEYIKLWGPLMAGYAIASIPVIILFTFSMKLFVKGLTE
                     GAVKG"
     gene            38244..38831
                     /locus_tag="PKI64_00175"
     CDS             38244..38831
                     /locus_tag="PKI64_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99451.1"
                     /translation="MSMEYSAPFFGDEIPLDHHPLEIRLRSLGQLFDSHDPSPFLAKD
                     LADDAQDYIIASAEEVADRLPLALVIHLSEPPTSPAEARDAGEAIREHFVRQSGLTRR
                     QFRSLIHHGLISLAIGLAFLSLALITSAALTGWAAASTLRDVFREGLIICGWVAMWQP
                     LQIFLYDWWPVLGERRLYDRLSRMPVQIVVGDSPA"
     gene            38931..41027
                     /gene="pta"
                     /locus_tag="PKI64_00180"
     CDS             38931..41027
                     /gene="pta"
                     /locus_tag="PKI64_00180"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOD99452.1"
                     /translation="MSRNVYIAATEASSGKSAIALGVMEMMVEKVGHVGFFRPIIPAD
                     RNGAEQDNDIALMISHFRLDLPYEKAFGYTSREASELIRAGKTSELLDGILERYNEMA
                     RRFDFVVCEGSDFVGQTSAFEFNINAAIAKNLAAPVLLVVDGQKAVSDGRMTTLDFAI
                     QSFEENDCTVLAAILNRFPPERLTELPDLLANTRHASRPIFAVPDDPGLAAPMMSEVA
                     HALGAETFYRPDLLDSLQATSFAVADSSPELFLSQLRDGALLFISGDRFGNLLGVMLK
                     LNSARPVNIAGVVLTNGFRPSGAVATLLKEVRNMVPVLSIPGSTYEAISIVSRLHSRI
                     YPGATRKITAALALFESNVDTQKLVDLLETAQTDVVTPKRFEFGLIQRAQEPRQRIVL
                     PEGEEDRILYAAEQVLLRGVADVVLLGSEQKIRGKISHLGLNLPGVEIIQPTQSPHFR
                     DFVSSYYELRKEKGVTPDMAETIMADASYFGTMMVHKGLADGMVSGSVNTTAHTVRPA
                     LQFIRTKPGFTIVSSVFFMCLKDRVLVYGDCAINTNPTAPQLAEIAVTSARTAATFGV
                     DPRVAMLSYSTGDSGGGADVERVREATELARAMAPDLPIEGPIQYDAAIDLSVARTKM
                     PDSKVAGRATVFIFPDLNTGNNTYKAVQRSAGAVAIGPVIQGLRKPVNDLSRGCTVVD
                     IVNTVAITAIQAQAEK"
     gene            41024..41974
                     /locus_tag="PKI64_00185"
     CDS             41024..41974
                     /locus_tag="PKI64_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOD99453.1"
                     /translation="MIPLSLSTRWNAGRHQSGQALIEEILELGLGIVELGYDLRAELI
                     PGVKAMVASGAVRVDSVHNFCPVPIGAPRPHPELYTPASPDRREREFAVTHISRTLRF
                     AAEVGARVVVCHSGNVDIPKLSFELTRLAARGEQFSAPYENLKLKAQLARDKKAPRQI
                     GYLTESLQQLLPVIQETGVRLALENLPTWEAIPTELEAEKLMKQFYAAGVSLWWDIGH
                     AQIRENLGFINASRWLQRLEPYIAGMHIHDVLPPGQDHVMPPRGKVDFPALAKIAGMD
                     MVRVLEPAPNTETAHIVQAIAYLRETWNLPDVNTTSGETT"
     gene            41971..42945
                     /gene="gmd"
                     /locus_tag="PKI64_00190"
     CDS             41971..42945
                     /gene="gmd"
                     /locus_tag="PKI64_00190"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD99454.1"
                     /translation="MKKALITGITGQDGSYLAELLLAKGYQVTGMVRRASTETFDRIA
                     HFKDQISLRQADLLDQFSLVKLLDEVQPDEIYNLAAMSFVPTSWNQPVLTGEFTALGV
                     TKLLEAMRMVCPKAHFYQASSSEMFGKVIETPQTETTPFYPRSPYGVAKVYGHFITVN
                     YRESYNLFALSGILFNHESPRRGKEFVTRKISDAVARIKLGTLKELRMGNLDAKRDWG
                     FAGDYVRAMWLMLQQDKPEDFVVATGETHSVREFLEIAFSHVGLKYEDYVVIDPEFIR
                     PAEVELLLGNPAKAKATLGWQPKVSFEQLVTMMVDADMDRQMGKTVTA"
     gene            42998..43921
                     /locus_tag="PKI64_00195"
     CDS             42998..43921
                     /locus_tag="PKI64_00195"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD99455.1"
                     /translation="MKILVTGCNGFVGRHAQAALRAAGHEVIGCDVACPPDSACLPLD
                     VRDLPAVCRLLTNVCPDAILHLGGLAFVPLGWTEPQRVFAVNTIGTLNILDAVRQTAP
                     ATRVLVVTSAEVYGSHPAPAPLTEDAPYLPDNIYGVAKAAADQSALLYAAHHKLAVMV
                     ARPSNHIGAGQSKDFVSTAFAAQLAAIAAGAPPVMRVGNLDQQRDFTDVRDVARAYAL
                     LLAQGRPGLAYNIASGLMRPVRDILTGLCEISGLHPRVETDPALFRPNRDRPAYDTSR
                     IRQHVGWQPLIPLRQTLADIYADIQAQRASS"
     gene            43953..44936
                     /locus_tag="PKI64_00200"
     CDS             43953..44936
                     /locus_tag="PKI64_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD99456.1"
                     /translation="MSSRHPILGQFARWAIGLALLAVLLRTIGLDDVRQEFARVQDAP
                     GWLAAALALTFLALFAGVVRWHLLLRAMGLPTPFHRSFRGFFIGQFFNAFLFGACGGD
                     LARAVHAAHAHPARRAEAVTSVFLDRVLGLLVTLVIGCGLLLLRFRYLANDPHASRAG
                     WLMMAFLAAATGGLLLLFSHNIFARLPWLARWQHRGWIGPLVRRMYDALFFFRQHGRH
                     LFWPTVLSVVNLLLLAAASGALARAMNLALPFMDLLTVFPIITVLAAIPITPGALGVR
                     ETLFIQLLQPFGVAAGAAVSLSLLGYFAGSAWSLFGGLLFLLAPRPTNPPP"
     gene            complement(44944..45969)
                     /locus_tag="PKI64_00205"
     CDS             complement(44944..45969)
                     /locus_tag="PKI64_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOD99457.1"
                     /translation="MKRKTIKDIAKESGFSLSTVSLVLNNHPRISQATRERVMAVVKK
                     YNFFPNSAARGLASKSSRTISVVVPNLTRVFSDIYLGEIISGIYERAASQGYKILLDI
                     ATAQFIKSQEYTKLLASRRADGMLFIASDIHAEYLTAFENSPYKFLLVNHLFPGKKLN
                     YVSVDYVASARLAAEHLLGLGHRKIGLIAGTNTYTGLQFRDEFIRVCKSWPAGTGQVA
                     SVDGGEEWSQRGGYAAAAKLLAMEPNLTAIMAANDRMAIGAMRWLVDNKREVPEDVSV
                     MGVDDIQLAAFVNPGLSTIRHDLHQVGIESCNRILQLIKGEITECSDLLPTSLVVRGS
                     TSRVAGK"
     gene            46169..47530
                     /gene="mgtE"
                     /locus_tag="PKI64_00210"
     CDS             46169..47530
                     /gene="mgtE"
                     /locus_tag="PKI64_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOD99458.1"
                     /translation="MRNPILVPELLEFIQAGNPAKVQEVVDHVHPADMAIFIAALEDA
                     DVWRLLAMMSIQHAAAIFSHFDLERQVELASDANRAEMARMLEALPHDDRADLINQLD
                     PQVADQLLPLVARAEREDIRNLTSYEEGTAGAIMSSDYAALRPDITVSQALDQVRIQA
                     PSRETVYYIYVVDADHRLIGFVSLKDLILASPVQQVREVMHTEIIQARAGDAQEHVAQ
                     LIEKYDLLALPITNAEDRLVGIVTMDDVLDVQEAEATAAFHKIGGTTLADENLKEAGL
                     ALLYRARLPWLLVLVFMNVLAGAGIAYYENTIMAMASLVFFLPLLIDSGGNAGSQAAT
                     LMVRALAVGDVRMRDWFSLLRKEVLVSLFLGLTMAAGVMLVAQLHSPEIAIPVALTMV
                     SVVLFGSLVGMSLPFLLTRFKLDPATASAPLITSLVDIGGVLIYFGIATWWLKDAIHA
                     AGS"
     gene            complement(47617..50568)
                     /locus_tag="PKI64_00215"
     CDS             complement(47617..50568)
                     /locus_tag="PKI64_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOD99459.1"
                     /translation="MKSLSCCALKNGLVLSLLGMGIATGAWGSGPPLHILNFNVSSNV
                     GSGNALHVAVDRPELNGMTTTGSVRMVSSNNQWSAKVAVQGSTVSTQVTYRFTPRTTS
                     SGSYCTTGNGNLSGTGLTTNVPLWNPGYSGKTIYYHTTWTNVTVFHRVGETETWQHSA
                     VMGDVGAGRGGNERRYKVTGVGEPGRALEFVMHGYDGGVEKWDNPAVGGVGDPPNYYT
                     WLDAFFVQDGNIFNYVPPATVSAPQVINVGNWASSYAGNGIPSRGGRIYLPRGYTQNS
                     SKRYPVLYMHDGQNVFDPGGAFGSWSADQAATREITQGRMRETIIVAVNNTTNRMDEY
                     GTPEDGHTGNYYLLYLINNVKPNIDANYRTLTDMMNTGNMGSSLGGLISAYIGLKTNV
                     FGLIGAVSPSYWYATNFRNWIRDNETKGRRIWQDCGTSEGSSMWDYFWPVYGYYLQDG
                     YVVGDDIRIDIGCGHGHDEWAWSQRVGAAFHYLYNPWDEPNELLEESEPEPQPGKLEF
                     STAATNVAENAGTVRVFVNRVGGADGAASVNYATSNGTATAGSDYTAKSGILNWADQD
                     NSQKYVDITILDDAVYEGDEVFYVNLSGVTGASLGSPNRIAVTILDNELPPPQLVITN
                     PAGNISVGGEVTTYTVQGTLMANNWQGMFWSNNLTGSSNAIPLSANWTAANVPLNEGT
                     NVITVTATNTQAIMEPIAEDIASNAAYLGDWLTGQNGGSGFGAWSLANNSEAGFFTSE
                     NGWGLWAHTNNLAEAIRPFVAPLAPGQQFQATLKNGWVLEGQQGVGMMLRNQQGADAI
                     QFYFNGGDVEYQIEDADGGRNSGIPWTDMPQTVTITLLTTNSYELRVGSAVFSGTYNG
                     PLVAARWWNWNGGEGSNYDFFFDDIKVLSLGSTVVSTSAVVTITRAASGVPNITNIQL
                     QGGETGGLNLSLLTSQAGATYWLWESPTLFPSSNWQKVTGSGRPGTGGAIDLSITNML
                     LPQNFYRVGIE"
     gene            complement(50662..52170)
                     /gene="cls"
                     /locus_tag="PKI64_00220"
     CDS             complement(50662..52170)
                     /gene="cls"
                     /locus_tag="PKI64_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOD99460.1"
                     /translation="MSGVAWTDYFRGAPWLSAGAGIHLLLFLAVALHVLRHRRRADST
                     LLWLFVTWSMPVIGALLYAMFGVDRVPKERWRRSVARSERMDDAQTMAGAEIVPEAYW
                     RSMGRAAATPADEWIQELDRPLAAMLENFPLLGGNHVTPLLTGDEAFPAMLAAIRGAQ
                     HHIHLQSFIIGNDPVGREFMEALAVKAREGVRVRVLYDRFGSSHALWGGLFRRYRRVP
                     NMAIAGWTQSNLFRKQLHFNLRNHRKTLVVDGRVGFMGGVNLNGYSRSQPGAPAIQDY
                     HFQVQGPVVQELQYSFLRDWHVMTEENPDQLLSAAYFERQPPAGQAFARVVNSGPSSD
                     LRLAVDVFFNAVNLARQQVFVITPYFLPSEDLLRALRMAALRGVRVVLVVPKTSNHWY
                     TTWAARAMYEDLLEAGVQINERRPPFIHAKAMVVDDRMAVVGSSNWDYRSLYSNYETS
                     LVVYDTELVMNLKLIMIEDLAASEPVDLAAFKQRPIRHRYLENACGLLAPLL"
     gene            complement(52258..53181)
                     /locus_tag="PKI64_00225"
     CDS             complement(52258..53181)
                     /locus_tag="PKI64_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD99461.1"
                     /translation="MTTTANSMVPDVVVVGSVAFDSVQTSKAAHEKLLGGSASYTSIA
                     SALFAKTGVVGVVGMDFPPEYFEIFRTAGIDLQGLQQAAGKTFHWSGVYEDNMNNRRT
                     NSTDLNVFADFNPVIPEAYRTSPFLCLENIAPALQARVLDQMAKPKFTVLDTMDLWIN
                     TTREELMNVIRRVDLLTVNEHEARHLTGKGSLLKAARKILEMGPKYALIKKGEHGAFL
                     MSHDDILIVPAWPLVEVVDPTGAGDTFAGGLIGTLAALGDVTRENLHTALVNATVAAA
                     YTCEAFSVDRLRHATRAELEARARAFRRMLP"
     gene            complement(53215..53781)
                     /locus_tag="PKI64_00230"
     CDS             complement(53215..53781)
                     /locus_tag="PKI64_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOD99462.1"
                     /translation="MDWDVFAEGHLDVAHEVLTTLRTPIEAVAVGMAERLRQGNKIMA
                     CGNGGSAADAQHFAAELVNRFQRERQPYAGLALTTDTSTLTSIGNDYNFDQIFAKQVQ
                     ALGRPGDVLLAISTSGNAANVCRAVEAAKAAGVWTVALCGGKGGALAAMADAVLRVGC
                     TGVTARIQEGHIMIIHALCQLIEDKLAD"
     gene            complement(53793..54338)
                     /locus_tag="PKI64_00235"
     CDS             complement(53793..54338)
                     /locus_tag="PKI64_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99463.1"
                     /translation="MRAIHFLLGLLLFLGIAAAGLVLIVSPAYPECLKLAVGFGASLP
                     PWVRVVAGVLVLGYLFIYLLSGVAWRRRRTFITFENENGQINVSADAVRDYLGGLKTE
                     FAAVVWLKSLLRVHRGALEVGLILGVRDGTRIPELCKLMQARVRELLAEHLGTCDLAG
                     VSIEVNEIQRRKKSTPDSAVP"
     gene            complement(54422..55045)
                     /locus_tag="PKI64_00240"
     CDS             complement(54422..55045)
                     /locus_tag="PKI64_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99464.1"
                     /translation="MKRRRWLLAVSLGWLACAPAQDELTAYFLAHGAQDPAVAAEDAY
                     KYLYHAARGGEHALADETAAGLWLAREWATLGPPQPGEELWEPLTADGRIGRLHLRPY
                     KAQGGNKEALLEAFLAGARDFDGHAGSFRQAWRALGRVLQAQPVGQLTFAEWQRVDRA
                     MKSKGYPAAHHSVGYTEARAPAYRVLPAAQARRLLEQLPEPVTISAP"
     gene            55124..55558
                     /gene="nikR"
                     /locus_tag="PKI64_00245"
     CDS             55124..55558
                     /gene="nikR"
                     /locus_tag="PKI64_00245"
                     /inference="COORDINATES: protein motif:HMM:NF001884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOD99465.1"
                     /translation="MKKSNNNKSDCAARLSLSLPTPLAADLDRLVRAKGAPSRSALLA
                     DLVREAVTEHDAHRGRQTAAGVITLIYDHHARNLQAKLTGIQHDAEKMIISVMHVHLS
                     HHDCLETIVVRGPAAHLRQLTDRLAAVRGVKHGKLTITTTQK"
     gene            55565..56656
                     /locus_tag="PKI64_00250"
     CDS             55565..56656
                     /locus_tag="PKI64_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99466.1"
                     /translation="MKVKTPIITPVLWLALILLLVMGMTALAQLPVVTHAELQAVNST
                     GHTAWVESFPFVIRGVIINDPEEMLDYSYDPDATVSNRLGGQYQMFFQGIGGDRGGTA
                     LWMAQNYQAMGQAGQDYGTEWTNEMRRIQYDANGRKFRKGDLIEVTARKALFRGGKRN
                     INEAHRITESNDFDVVLVKANVGVPSAEPITLADLVNPDGTQIFDATRATGGEHWQGM
                     RVRLDQIRLSTTNGWGKTNWADRICLAADQSGRTFPLRMPLLDLGPPKATDVWFSVTG
                     IINQENSNTNGYELFVQEVGPELRITQGANGRPAVSFSSDYDGYVLQYSDDGLNTWAD
                     LDATPVKTIIIEDQGDSINRMYRLIKKEE"
     gene            56656..57285
                     /locus_tag="PKI64_00255"
     CDS             56656..57285
                     /locus_tag="PKI64_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD99467.1"
                     /translation="MRRGFSIIELTVVLAALALLLALAAPMLARAIEQARVARVVKDL
                     QTVEVALEAFRTDHGRYPPVVMSCDNSDQQEVLQLPLELADGNYLPRSPRSASSSLLA
                     DPFRPRSTYKYVAPENYWMNGSFQDDRYPVWVPNDFPSCHAAGGKADDSPNSPVAWAV
                     WSPGPRPNQLKVPSYKLPLAAEGWYTKPGDYGIIARYKVRNGATWSSLR"
     gene            complement(57346..59121)
                     /locus_tag="PKI64_00260"
     CDS             complement(57346..59121)
                     /locus_tag="PKI64_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOD99468.1"
                     /translation="MIHIRINGRDLAVAEGSTILQAARQAGIHIPTLCYLEQRDPLGA
                     CRVCLVEVEGARTLVTACSAPCTEGMVVHVNSARARNARKQVVELLLSEHDGNCQVCE
                     RNDDCELRRLASDMGIRTVTYEGAKARPKHDASTPGLVRDNAKCIKCRRCVTVCSEVQ
                     GVGALFPQGRGFDTVIGPAFAGDLDGVTCVQCGQCAAVCPVGAISERSHIDEVWQALG
                     DPAKTVIVQTAPAIRAALGEEFGYPPGTRVTGKMVTALRQMGFAAVFDTNFAADLTIM
                     EEGTELLSRLKRALMDKDPTAALPMFTSCSPGWINFAEYYYPNLLAHLSTCKSPQQMF
                     GAVAKTFYAAKIGKRPEDLVVVSIMPCTAKKYEAQRPEMTASGVQDVDYVLTTRELAR
                     MIRQANLAFTELPDSKMDAPLGLSSGAADIFANTGGVMEAALRTAYEIVTGRELPADN
                     LHIKPIEGLEGIKEAVLTITDTTQQWAFLEGVELRVAVAHGLGNTRRLLERINAGEVQ
                     YHFVEIMTCPGGCIGGGGQPRFTDDSVRLRRIAAIYEEDESKTLRKSHDNPAIQELYR
                     TFLGQPLGPKSHALLHTHYCAKARR"
     gene            complement(59118..62252)
                     /locus_tag="PKI64_00265"
     CDS             complement(59118..62252)
                     /locus_tag="PKI64_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOD99469.1"
                     /translation="MTARLKKIGTAAALAAWRDQLRTAPVPKHELRVCVGGSCLAAGA
                     QKVVDALQSAVAEAGVAKDVAIRTAGCMGPCVRGPVLRMDDGTFYQGVKPEDAADIVR
                     EHVQAGRVVERLCASDSQTNALQPKTTDIPFFTRQTQVVLRNCGWIDPERIEEYVARD
                     GYAALAKVLTSLTPEQVIEEMKSSGLRGRGGAGFPTFMKWDFTRRAPGTVKYVLCNAD
                     EGDPGAYMDRSTLEGDPHSVIEGMAIAAFTIGAAQGYVYVRAEYPLAVARLRKAIDQA
                     HACGLLGNNILGSGLSFDLDIRMGSGAFVCGEETALIASIEGLRGEPRPRPPFPAQKG
                     LWGKPTLLNNVETYANVPAILLNGAAWYAQWGTAKSKGTKVFALAGCVQNTGLVEVPV
                     GTSLGELIYDIGGGLRGGKAYKAAQIGGPSGGCIPKENLNVPLDYESLQELGAIMGSG
                     GLIVMDEDACMVDVARFFIEFVQEESCGKCAPCRVGTKRMGEILQRICEGKGELADLD
                     RLEQLGRFIQEASLCGLGQTAPNPVLSTLRHFREEYVQHIRDKHCVAGVCPGLVLAPC
                     LSACPAGVHVPGFVALVGEGRYAEALQLHRERNPFAAVCARVCFHTCEDHCRRSTLDE
                     AVAIRSIKRFMVDQEVTIVKPEVRENEANAARKVAIVGAGPAGLTCAYFLARLGYKPV
                     IFEAEARPGGMLVQAIPAYRLPRETLAREIRMIESLGVEIQTLRRLGRDFTLPDLRQE
                     GYEAVFLAVGAPEGLNLAIPGHEAKGVVDSLDFLRQYNIRGSVPVGKNVVVIGGGNAA
                     IDAARTALRLGAQKVTVVYRRTREQMPAYVEEIEEAQQEGIELRTLVAPEEFVVRDGR
                     VTGLVCRQMTLGEFDRSGRRRAEADDEQALETIPCDQAILAVGQRLDARNVLGDLEVP
                     LAGGWLQADPVTGQTAIPWLFAGGDAVSGPSSVVAAIGAGERAAVGMDALLTGETHAF
                     WRTYPDVPTDYDPEADPAPYPRENPNLIALDRRRNNFDEVEQPWSEVTAQRQAHRCLR
                     CDYGKTGQVRGLAT"
     gene            complement(62249..62791)
                     /locus_tag="PKI64_00270"
     CDS             complement(62249..62791)
                     /locus_tag="PKI64_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOD99470.1"
                     /translation="MAKPETCAGCAAAAAHDEAELLARLDEALAMQRDKPGGLIPALQ
                     MAQAIFGYLPEVALRRVAAAFDKSYSEVTGVVSFYSFFSTVPRGRYLVRVCLGTACYV
                     RGGSEVLRALQAKLGIGVGETTPDRLFSLEVGRCFGACGMAPVLMINDDLHPRVKPAK
                     LGELLESYRARAQGAETEPS"
     gene            complement(62846..63292)
                     /locus_tag="PKI64_00275"
     CDS             complement(62846..63292)
                     /locus_tag="PKI64_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD99471.1"
                     /translation="MSTTVGDLLRRKGSQVETITAQESILVAIRRMSEKHIGCLAVVT
                     KTGKLSGIISERDCLWKVIAAGKSPKTRLVKEVMTSVAKMHTVTPQQTAEECMGLMTS
                     GRHRHLPVLADGKLAGLISIGDIVKEMLDSQQATIASLEKYITGTF"
     gene            63927..64889
                     /locus_tag="PKI64_00280"
     CDS             63927..64889
                     /locus_tag="PKI64_00280"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOD99472.1"
                     /translation="MNFKSRKVAIVGAGAVGSGFAFALAQSGIAEEIVLVDANTDLAK
                     GQALDLAHGAPFYRTVSIYADTAPQAYRNAAIVVITAGKAQAGPSQSRLELMQINAKI
                     VTSIATDIQMVNPEAIVLVATNPVDIVTHVARLRTQWPRGRILGSGTVLDSARFRWLL
                     AEHCGVSPQNVHASILGEHGDSELAAWSLVHIGGLPMDHYCSVCRKCDFIHRRPQILE
                     QVRRSAYHIIGYKGSTSFGVAMAMVQIVRAILRNERRVLTVSAPLAGEYGLNDVTLGI
                     PCVVGANGIEEVIEAQLTPEENQELAQSAQVLKDSLALLETSPA"
     gene            complement(64902..66074)
                     /locus_tag="PKI64_00285"
     CDS             complement(64902..66074)
                     /locus_tag="PKI64_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOD99473.1"
                     /translation="MQTTGSSLPSSVTKVELEGKTIYIVGTAHVSAQSVADVRETIAA
                     VQPAAVAIELCPARYQGMVRKDAWQRTNLFQVIRQGKAMFLLAQLIMQSFYRRLGKQL
                     EVEPGAEMLAAAAAAEASGARLELIDRPIDITLKRVWRHLGFWQRVKLLGVMIEALFS
                     GDDVKDEDIESLKKQDQLEALMKEMGKAFPQIKKRLIDERDVYLAQKLRAVSAPTIVA
                     VVGAGHVPGMVGALQTEVPLAELESLPPRTRWSKFWPWLIPALVVALIVWGFIHGGAA
                     KGIDSILIWVGVNGVLAAIGAAVTLAHPLTIASAFVAAPITSLNPTIAAGWVAGLVEA
                     WLRPPNVADFESLPAAMESFRNFIRNPVVRILLVVVMTNIGSSIGTFVAIPWIAAH"
     gene            complement(66083..67345)
                     /locus_tag="PKI64_00290"
     CDS             complement(66083..67345)
                     /locus_tag="PKI64_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD99474.1"
                     /translation="MMKMMKWMLAVGLMVSGVTYSAVAMDDDLFARAPWFGTLGVSYY
                     HLEGDVEAKPGVGLMGKLGYSLNSWWDVEGGVDFMPSLSARPASKLNTTPLDGSTTAL
                     RLSLDALLHLRVLENRRVDPFLRAGPALTFFGDDMDNGKTQFGLFAGGGLFYHFNDSW
                     ALRGDVVAGAQGKDWEFTALVELGVCYRFGAERVVAPVFQVAQGGDIDSDGDGLFDRD
                     EAIIGTDPYNPDTDGDGLSDGDEVFKYKTDPLNPDSDFDGLKDGAEVLTYKTDPLNID
                     TDGGGVSDGHEVIEDGTNPLDPLDDLQKFTLLIEFDYDKSFIRPQYFADIEPVVKVLQ
                     RDPNATVRVEGHADKRKTSKRDYNQRLSERRANAVKDYLVQQSGINPDNITAVGYGFD
                     RPVAPNDTEENMQKNRRTDIYIKKGGAQ"
     gene            67494..68186
                     /locus_tag="PKI64_00295"
     CDS             67494..68186
                     /locus_tag="PKI64_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD99475.1"
                     /translation="MSTLFDQYGENYDAAMTKSIGFMGQSHDYYTQAKAVHILSILRR
                     HLGAPRQLAVLDVGSGVGKTDRFLSGGIGHLTGVDISATSVERARRENPSVRYEVYDG
                     RQLPFPAESFAAAFVICVMHHVAIPDRVALLQEVRRTLIPGGLLMIFEHNPLNPLTRL
                     AVARCEFDRDAQLLTRGLSARLLKQAGFTIAEKRFILVSPFAFKGSGCLERAFCQAPL
                     GAQYYVVGQKTG"
     gene            complement(68198..70150)
                     /locus_tag="PKI64_00300"
     CDS             complement(68198..70150)
                     /locus_tag="PKI64_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99476.1"
                     /translation="MTRRGIQWLAWGAVVVLAGWVRFADLGYTPTRSDELNMLNYAGG
                     ATTLAKLWTDPPWLNQIPLVDSIPVVWARLQPWRTVDEAFTREPFALLGLLTVAFCTG
                     WMLRRRGLGAGLLLGLWLAMLPYHVYHSREAYYYVVTMFFAAGMLLRGADFMTQLRGG
                     TELKARQYGEWAVWTLLACLAHMSAWAVAGITWALLFVTGWRGLWSSARRRHSIAMAW
                     VAGGLAVGMSRWIFRAFLEMQRTAASPMGHIGADFGLVGPRVLPFFAGGANWVGGALL
                     VLLLAALWWNVWRARKTGAAADSLYAAISWLAWLGLAASYAYVFGVGGGGKAKLVYFA
                     VNLPSFICWAAMTLERAATAWGGHRQTLTLAALGLLIAAALAAPVWHIMHLAGKPTPY
                     RLIRDWLDTNMSPGDVVLVDRWLEPWNEIARYMPRDVTVGFTVPDEPYEQYVALNWRK
                     RTQTLIEQNGAQGFLRLARTHADRIGVWTWPEKWFAHRVVLTNATGVWLRDTGFAPME
                     EFYTETSRVEPELFYSTHEELAEHAKANGQTACLFFGDGWRLFKPWQQGDFTDYRILE
                     DAATLEVHNVSGRTASFRGEMTAAGNGSSVTLRIGESNPLVFPAGQLAKQTFTVDLPP
                     GVHQLACKKLAGGALVVRELRMAAIP"
     gene            complement(70159..71112)
                     /locus_tag="PKI64_00305"
     CDS             complement(70159..71112)
                     /locus_tag="PKI64_00305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD99477.1"
                     /translation="MSEAKLITIVVPFLNEKDNLPILLERVAGVFAARRENWELLLVD
                     DGSTDGSTEWALDQTRTNPHVRLVRLSRNFGHQLAITAGMDRATGDAVVIMDADLQDP
                     PETIAAMLDKWHEGFEVVYAVRRSRAGETWLKKFLAASFYRVFRRLAKTNVPMDAGDF
                     RLVDRCVVDALREMRELHRFMRGLTCWVGFRQVSVEYDRAARLAGETKYPVWKSARLA
                     FDAITSFSASPLRWIAVLGFGVCVLATLWVIYVMVLAIVQPQSLEKGWASLIAVMAFM
                     GGVQLVSIGMLGQYVSRIFEEGKRRPLYLVRSDTRDPAGAK"
     gene            71289..72644
                     /locus_tag="PKI64_00310"
     CDS             71289..72644
                     /locus_tag="PKI64_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD99478.1"
                     /translation="MGTERVGRLLLRFSIPAIVGMLVQALYNIVDRMFIGYGIGPQGI
                     AGVTISFPLMMAMVSFSILVGVGANTLFAIRMGEGRREEAERILGNAFGLLFFVPLVA
                     SLVGLAYLDPLLRLAGASDELLPYARDYARIILGGTALTTTGHGLAHFIRSDGRPFIS
                     MAAMLIGAITNTILDPLFIFVFHWGMKGAAWATVLAQGLSFAWCFGYFLRSRTGTRLR
                     RHNLRLRPREIVWPLLGIGFTPFAMHLANSFLNVILNRGLREYGGDDAIAVMGILAAY
                     MSIIFMPVFGLAQGAQPLMGYNYGAQQYARVRRLFQISVLVATGFMVMGWTLSQLFPV
                     RILRLFVPADSALIPLGRHAMRVFTLAFPIIGFPIMAGQFFQAIGKPVKAALIALSRQ
                     ILLFIPFILIFPLFWGLPGIFFAAPTSDVMATAIAIPLVWRQLRLLRRSPLKEPRHED
                     V"
     gene            72631..73077
                     /locus_tag="PKI64_00315"
     CDS             72631..73077
                     /locus_tag="PKI64_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOD99479.1"
                     /translation="MKTCDTCGEEIAPQSSICPYCESPQAWSPPTTNGPAIKTINLEA
                     GLPTVEEALRRLAAQLDRARLEGVRVVRLIHGWGSAAGGGGRIRAAVRQWLQQEAEAR
                     RIHFFLPGDHFTNTTPRGRDFLSRHPALRQSIRTDRENPGITFVEP"
     gene            complement(73161..73784)
                     /locus_tag="PKI64_00320"
     CDS             complement(73161..73784)
                     /locus_tag="PKI64_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99480.1"
                     /translation="MKRMALIGILATVAAVAMVGCNWDTGNEATSWTDTYNWVNFSGT
                     YTPFTGNSYVIKLGGIHSIAVFHRGQNLEMRDDRGNLYTGYMKWIQSESGVLPTPAST
                     NGPTYPRADKTDRVVASYECTGSNGSTIVGTFEGYQVNNVLRDRIMLGTWVLGPRADT
                     IQGAAPAMVLDTRPTDTAFSQDGPIADGSGIIPDSPPPPTPPPTPTP"
     gene            complement(73821..74897)
                     /gene="hisC"
                     /locus_tag="PKI64_00325"
     CDS             complement(73821..74897)
                     /gene="hisC"
                     /locus_tag="PKI64_00325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOD99481.1"
                     /translation="MNPRIRQTVEQLTAYTPGEQPQVPGLIKLNTNENPYPPSPAVTE
                     VLRTVAAESLRLYPDPVCSALRVRIASLYGCAPDQVFVGNGSDEVLRLCTRAFTQPGG
                     TVASFAPSYSLYPVLAAAEEVGFWEVPLPDDFSWVAPPGDLAATLFWVANPNAPTGVF
                     YPVEAIREFSERFTGVVAVDEAYVDFAGGRDCLTLARELPNVLVCRTLSKSFSLAGLR
                     LGFALGAPELIAALYKLKDSYNVDRLAQLIALAALNDVQWMQANARRIVATRERVTAA
                     LQQRGFYVVPSAANFLFAQPPDGLPAEQLFAALRQKNILVRYFPGARTGRFLRISIGT
                     DAEMDALLTALSEIPVDGSAGKRV"
     gene            complement(75015..75485)
                     /gene="rpsG"
                     /locus_tag="PKI64_00330"
     CDS             complement(75015..75485)
                     /gene="rpsG"
                     /locus_tag="PKI64_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD99482.1"
                     /translation="MRRRRAESREFAPDARYGSVLVTHLTNIIMSRGKKSTAQRTVYG
                     ALDQVKEQTEGKDPIEVFQQALENIKPKVEVKSRRVGGATYQVPVEVPASRQTSLALR
                     WIVDYSRGRKGASMQRALALEILDAFKNQGNAIKKRDDTHKMAAANKAFAHYRW"
     gene            complement(75498..75878)
                     /gene="rpsL"
                     /locus_tag="PKI64_00335"
     CDS             complement(75498..75878)
                     /gene="rpsL"
                     /locus_tag="PKI64_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOD99483.1"
                     /translation="MPTINQLVKKGRQPLSSKRKSPALQRCPQRRGVCLLVKTMTPKK
                     PNSALRKVARVRLTNGEEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYHIVRGTL
                     DCLGIESRRRSRSKYGTKTPKAAK"
     gene            76082..77185
                     /locus_tag="PKI64_00340"
     CDS             76082..77185
                     /locus_tag="PKI64_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOD99484.1"
                     /translation="MNRITSALLITLALAVAATAQLPSPSRPGPLRVAMLSDGGSFSD
                     SSFSQNCREGLEELLYSGTPIYVQFAEPITPANFERKLTALAERDYRLILGIGYRMAD
                     AVQNVAASYPRTLFASVDGDFEDIPHNVWVLTFQVDECAFPAGYLAAAWADLQDPQDP
                     AIGWVGGVDCESVNQFVVGFRNGAQHYNEIKNRQVRILGDYVGSFGDFAGGKKLALKL
                     LDDGADVIFGVGSISGNGGIAAAKERGKWAIGVDTDQYNTLVDYRDVLLTSCLKRMDR
                     AVKQVVIAALENQFWGGSRYIGNLTNHGVGLASYHDYADKIPERLQKEIVDIQRRILD
                     GSIETGWPAKPAVQKKARAKAAEPAAAAAAAAP"
     gene            77182..77988
                     /locus_tag="PKI64_00345"
     CDS             77182..77988
                     /locus_tag="PKI64_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOD99485.1"
                     /translation="MITPAFSRLFGARRKVVIGMIHVRPLPGTPRHAHDWPAIIQTAL
                     AEAETYRAGGIHALMIENMHDIPYVKNPGPEITAAMAVIAREVKRAHPALPLGIQLLA
                     GANQEALAAAAAAGADFIRAEGFVFGHVADEGYFDSCAGALLRYRRHIGAESLAIFTD
                     IKKKHSAHAITADVDIVQTAHAAGYFLSDGLILTGAATGEAASLDELRAVYAATQLPV
                     FVGSGITADNVADYLPCADAFIVGSHFKQDGYWENPLDPARIRRLLAAVN"
     gene            complement(78029..80578)
                     /locus_tag="PKI64_00350"
     CDS             complement(78029..80578)
                     /locus_tag="PKI64_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99486.1"
                     /translation="MKKLGLGLVLAILLPWGAAWAAMDDADEQLATAKLTIEEARKLL
                     VMGRAAWEDGLYPLAQRKLEELVDGAPDRRRQAEGALWLARVQLAAGRPQEALATLTQ
                     HGGLARAQAMQAEYALTRAQVHQALGQIAEAAAELADFAKLYAGQPTVPAALLLQVQL
                     AVAQQDWPAAQTMCRALGEAFAGQSEEPQAWLAVGAGALAAGQRDVARTALTFVETNF
                     AGQAWSEQAALQLIEMQLAQGEQTGAVAKAQALLANTNVAVATRAAANLVMARLAAAA
                     SNTTEAITWADRSLALATDPAQRLAAQAVKAEILLQAGQTEEGVRLMRTTAAQVPDEK
                     LSARLQLQLAEWLAQRTQWAEAIAEYQAWLAAFDGNEGTARALAGLGNALWAAARPEE
                     AAAALERALSLTTDATQRATRLHQLGDARFASRQFAKAKEAYAAALAATSSGSDAARL
                     AQYQLAETELALGETEAGETRLLDLSRTTESADMARRATMRLGALYEERGALEAAAEQ
                     YRRVTESCPAGADCSDALLAHGLIRYRMGQFQAALDDFTRISETQPAAPAAAQAMFMR
                     GWCFYLLGKDAEALQVCEDFLAQYPASRFLPDVQFWLGEYAFNAGNYAEAEQRFAQVA
                     VEHPESARAPDARYWAGRAATASQNYLIANEHYNALMKDYPDSPRLPATLLAQGDVLS
                     ELGQFTAAVVAFDEVIKKFPRQVETIVALGRKGDCQYTLGQENPARYEEALLTYREVL
                     RDAHTPFDLRIQASFKVGRCLEKMGRATAALDRYMEVVYAFLQEEHPPEEAAVWFTRA
                     AFAAAALQEAAGQWKEAANIYRRVTAAGVPAAVEARLRMERIRREHWAAFQ"
     gene            complement(80575..83451)
                     /gene="uvrA"
                     /locus_tag="PKI64_00355"
     CDS             complement(80575..83451)
                     /gene="uvrA"
                     /locus_tag="PKI64_00355"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD99487.1"
                     /translation="MSNNCILVEGAREHNLKNITVRIPRGSLCVVTGLSGSGKSSLAF
                     DTLYAEGQRKYVESLSAYARMFLEQMQKPEVDRIEGLSPAIAIEQRSAGANPRSIVAT
                     TTEIHDYLRLLYASIGRQHCHQCGRAITRQSAEEIVNLLLKKDAGTKVQILAPLVEGR
                     KGEHAEVFENARRQGFTRVQVDGEIYELEKPPKLDKQKKHSIAAVVDRLVISDKIRGR
                     LTDSVELALKQGEGILHALFQEPGGATAWTTQMFSEKNACPDCGISFDALQARNFSFN
                     SPYGACPTCLGLGQMLVFDEELVVPDGTKSLDNGAIAGWRPSGRREAIYRKGLLRAVA
                     KHYGFSMETPWDDLPAKIREIILHGSGDEEIPLGFWRGGSWRKYRKPFEGVIPNLNRR
                     YQETDSDYMKQKLTNFMSRLPCTACRGRRLRPESLACYVVGQNIMDVMALSAHAAMAW
                     FADLPLPEQEQKIAGEVLKEIRKRLMFLCDVGLGYLNLDRESGTLSGGEAQRIRLATQ
                     VGAGLVGVLYVLDEPSIGLHQRDNEKLLRTLKGLRDLGNTVVVVEHDEQTIRAADYVI
                     DLGPAAGVQGGEVVFAGTVPELLQSKTSLTAQFLNGEKEISVPAKRLPPGGDWLEIIG
                     AAENNLKNIDVRFPLGRITCVTGVSGSGKSSLVDDILRRALFRKFYGSKEKPGKHKRI
                     NGLTHLDKVIVIDQAPIGRTPRSNPATYTGAFAGIRDLFAQLPAAKMRGYKPGRFSFN
                     VKGGRCEKCKGDGILKIEMNFLPDVYVTCEQCRGRRYNQETLEVKWHGRSIADVLDMT
                     IDEALEFFTPMPQIARKFRTLSEVGLGYLKVGQPATTLSGGEAQRMKLSAELARRDTG
                     KTLYLLDEPTTGLHFADVDRLLKVLLRLRDAGNSLIVIEHNLDVIKTADYIVDLGPEG
                     GGAGGRVVACGTPEEVAADPASFTGEYLRPLLKMPRRTKKNSKS"
     gene            83596..84651
                     /locus_tag="PKI64_00360"
     CDS             83596..84651
                     /locus_tag="PKI64_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99488.1"
                     /translation="MLSHDGSQNGSPFPADPPPAIPPREHQRAPRRPWPPRQPTARRH
                     TIQMLMAAGFGVVLMFGLLMLAVRYAESDWHKKNMQARSHHGTAATPTAAATKATAAC
                     WKYTLPPAAVPHTLERLGGDAILPNHWLVLGRGLSNKTDPKLTLRALWLAMSVSGESV
                     GMNNDFGVAYLEQKRIGEAVTQFQAALQLHPGFPPTLFNLALCAIAQRNPAEASQWLG
                     RYLGQRPTDETALRLQATLLVQTGRADEALLLLEKFLRDQPPTQPLYLEAAQIAARLR
                     QNAKALRYLEVALNGNPIQAVVRTYQSALFRDIRLSGDGDDLAARLAKSARIAYSTPV
                     SEDTVLPIRVTPDALLR"
     gene            complement(84693..87149)
                     /locus_tag="PKI64_00365"
     CDS             complement(84693..87149)
                     /locus_tag="PKI64_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOD99489.1"
                     /translation="MESWIDDELQSMPRAAHPPRRRALVGITLAVIAGTATGICAKVS
                     AVWFLGVGAFLLLPLFVLVQHRWSVGLLMVATFCLVAAHARLATGGRSALSLAAHMAR
                     PMEYLHFVAVATEDAVPQPPRAGRADGWVMMARVEGLNRDGQWRRVDDRLRIVIRGGA
                     PAHGRLPRYGERWRMRGLVRANVPRRTGLFTLPENEAIVDSDRLTFVDDGQGNPLVAW
                     CMKQRHACREILGRGLEDFPDQRGVVQSLLMGYRADLPRALRRDFAATGTVHIFAISG
                     AHVGMVTVLIIGTLRAMGLPLTWWFPFLAPLLVLYTITTGAATSAIRACIMALLALAA
                     PFFKRRSDAISTLFAAALVILLFAPAQLGDLGFLLSFSAVAGLLAIPPVLDSAMHRWF
                     RRDAWQLPGEEPPPKRRWRAAAFNGVRAFNVTLGAWIATAPLTLYFFNLVSPVALVMN
                     LLVIPSAFVILLAGVLCLLFAPVSAMMAGIFNHAALAVALFLTKCIEWAAAIPGGHWF
                     MRSPPLWGMAAWYGLLVVVAIMARRVRGAMLLGGLALALLAAGWGVWEQQRCRLVVLD
                     VGSGNATLVQARGAAYLVDTGAAFRADDLMRALRREGVNRLQALILSHADAQHVGAAK
                     AVMAQLPVAELWLPQAIWPSPLMNSILTEAAAAGIPVRRLQRGDCGHWPGGLWWEVLW
                     PPAEMKMTCADDAALALRVARWGVSVIVAGDLGGEQEKNLARGGAAVAGSVLLAGRHG
                     DAQANSAPWLDAVRPRDVVISGGEHLQKNLPDEEVMARLTARNIAIWRTDQQGTIELD
                     WHGRPARWPAMRYTLRATRN"
     gene            87321..89819
                     /locus_tag="PKI64_00370"
     CDS             87321..89819
                     /locus_tag="PKI64_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD99490.1"
                     /translation="MEMGTSFGMGKYRWRLGWGALACILLIPCAWGARPEAYFSGTRS
                     DANVYVAPVASSIHKIAILPLKAPTELIGSSVADIVVTEMMRAGRYTLVERGQMSQVL
                     GEAELALAGLSESAAVEVGKMMGADGVVIGTVDEYGMVAHRGRSYPVVGVSLRLIDCD
                     SGRVMWSVGHARRGNDPAGTLSSHARAVVHEVVAAVVQNWRVQRQVGEKYDDARGTRD
                     RPDDLVGMHGDPVAPTAPAFEPPQAPDSVAISDFGLREVRLQWTPPADRSIQYRVERA
                     ESPAGPFAVLATLAAARGAYVDSGTARAPLQDAGTYYYRLTAIGPDGQESEPGGVLES
                     MTAPPPEPPAELQALTPAARAVQLQWAAPSSEGVTSYQVERAAASDEVFALVGTVKTT
                     TYHEGGTAASPLRDSTPYLYRVRAINRVGAVGEFSKPVEVITRPPPAAPQNVTAESQQ
                     VRCVPLSWQASQEEDVTHYIIYRSESDAGVFTSIGTVKGRDNTKYLDGGRDPGTLEDD
                     QAYFYCIRAVNAVGAESDDSEVVMAGTRPPPPRISRLAAQSGLPRKVALTWQPSDDEK
                     VIGYDLERSEAGGPFAPVGRLSGREIAEYEDGGGELARSWHSGNKNSLQDGMPYTHRI
                     RAVNTAGAVSPWVETAGATTKVVPAKPTGLKASERRARVIGLVWAANPEKDIAEYVVA
                     MSPFPDRNFTETARIPAGAMRGLKQENLPPDLTRYYRVKAIDADGLESEWSDVVCGTT
                     KPLPAAPAELAVEWEEDGARLQWTPPPERDIVRYRVLNKRLFGQDEISTCTSPSILIP
                     LATLAPKKVFIVIAVDKDGLESPPSPPLEARPPR"
     gene            90235..>91180
                     /locus_tag="PKI64_00375"
     CDS             90235..>91180
                     /locus_tag="PKI64_00375"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOD99491.1"
                     /translation="MDDFLPYLRQAFRQAHGLPLLVPPLAVPEPASRLLTWARRHRIR
                     NLIQAEFPTADEPVPTAAYGQVQHTARYVAEAERLHGLLAHRLRFLALVKGPSLAAQA
                     WPEPGLRIYDDLDFMCHPRDFLQLRVILKNEGYIPRARDARHLAHLWYFGWGVSFDHP
                     NGHMVEVNHRFFPPHYPWPVRRNRSWRGQFAPQRLDEAEVQAPTPALHLLLSCLHATW
                     HGWARLVWLVDIAGLLARHPEAFGQAQALAAGSPFAELVLAVGCQAAEELLGPGLAPT
                     ARELPATSEAESVLHLLDGTEAPLRRSHLRRFHETLMTR"
     assembly_gap    91181..91280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <91281..>92575
                     /locus_tag="PKI64_00380"
     CDS             <91281..>92575
                     /locus_tag="PKI64_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99492.1"
                     /translation="GSLIDNVNQLGKGGNLGYWGNWNSAYTGARQGGRLDLVGFTYHH
                     SLAPLLPKEIFRKELQIFKQAWWKAWGGNSDLSDHSKGYFPTEMAFSQTLIDVLVDEG
                     YEWAIVASHHLSRTCPTYFDQFNLAAGQYNIFSTPPNKADLLGPSPTSGWWYSEPNPG
                     NAAWNVAPFAYQLHKAQWVNPNSGATKQIYVVPSDDVLSYRFGYANEGIGKIQSFIAP
                     YATDPNRPVIVMPASDGDNAWGGGFSSWQEATPQFFNDSANAGYVKNTPQGFVNAAKA
                     NAPVTHVEDGAWIFPEMDYGSPNFLKWIEPPVNPNSIPTCYPNTMVDLETPGFALKFF
                     SYAPLMAGANWVITAEQILKDEGGSVQAWKVQDPYNNLGDGNWTSPNDVELAWHIYLA
                     GLDSGFNYYGGLGNDDEVKPALATKNAIGKLQSFMSTRL"
BASE COUNT        17275 a        30183 c        26711 g        18106 t
ORIGIN      
        1 ttaaacacct atctcaaacg cactcatcag gtgcagaaag gaaaccgatc gtgaagatac
       61 gtaacacagt tggaatggat atgggagaca aagttcactc gatttgcata ctggatgctt
      121 cgggcaccgt gcgggagtgc gccgaggtga gcaatacccg agcggggatt tacaagttct
      181 ttgccaagat ggagcccagt ttgattgctc tggagacggg tacgcactcc agttgggtga
      241 gccggattct ggaggaattg ggccacgagg tcttggtggg ccatgcgcgg aaattgcgga
      301 tgatttggaa caaccctcaa aagaatgata tgcgcgatgc cgagatgctg gcccgtattg
      361 cccgttttga tcggaagttg ctccatccga ttcagcctcg gagcatgaaa gcccagtgcg
      421 atttggcggc gatcaaggcc cgcgatcaac tcgtgaaagt acgcagctcg ttaattgccc
      481 atgtccgcgg tttggttaaa tccgtcgggg cgcgattgcc cacttgcagt ccggagtgct
      541 ttgccaagcg tgcggcggag gcggtgccag aagaattggc tttggcgttg gaaccggtga
      601 tcgtcagtat tgcggagctg acggttcgta ttcgccactg tgaccagctt gtccaaaaac
      661 tggcggacga ggagtaccca gcaaccaagc gattgcagga gattcccggt gtgggtccga
      721 ttacgtcttt ggcctttgtt ttgacgctgg aagatcccaa gcgctttgcc agaagcaggg
      781 atgtcggacc attcttggga atgaccccgc gccggaacca atccggacaa gtggatcaac
      841 agttgcccat caccaaggct ggcaatgcat atatgagacg attactggtg caatgcgccc
      901 aatacatcct gggccactac gggccggact gtgatctcag acgatttggc ttaaaactgg
      961 ctgaacgagg aggaaaaaat gccaagcgcc gggcaacggt ggccgtggca cgcaaactag
     1021 cggtgttgct gcacagcctt tggaaaaccg gtgaagaata tgagccgaac cgttgccggc
     1081 gccagaacag gaaaacagct taaaatgaac aagagaggga ctgcctctgc ttgaggtgcg
     1141 tttgtccaaa ccagggagac taaaagacag acaaccccgc tgaagagttc tttgacggcc
     1201 cgtctgggtg actgcggtta cacacttgga ctgatggaat gcttgccatc gcaaaaagtc
     1261 cggtccacag atagcagcac cctctagcgg accctaccaa gcaccggggc aaaagcggct
     1321 ccaacaagag tgcgaataag agcgcgaccc aaagaactcg ccaacgcggt gtccgtcaaa
     1381 acggacctct tgacatgcct tctcataaga gtgtggaaaa gtgcgatggg aggggccggt
     1441 cagggcgcga ggcgcaggtg gaagtaggtg gtgttcgtgg gcgcggggtt cgtccacggc
     1501 gaagtctgca ctcccagatt agtccacgga accggctcac tggtcaggct gtgggaacgc
     1561 cagacggtga ctccgcccac gtcgttatag ttggtccagg tcagcaccag gttgccattg
     1621 gtggcgcgga tggcggtgat gcgcggcggc gtggcgccca ggatggcgag gtcgaactca
     1681 cgatcggcaa tgccatattg gttgctggcc aagatggtaa agttgtagtt gccggcgttg
     1741 gtggggatgc cactcagttc gccggcgagg ctcaacgtga ggccaccggg cagtgtgccc
     1801 gaaagcaggg tgtagtccgg atcgccgctg gcgacgatcc agacagagta ggcggaggat
     1861 tcgcggccgt tgggcagcgg gttggtggtg ctgaagaccg ggcccgcgcc gatgttgatc
     1921 gagtaatcct tgttggtata gccgaagtcg ttggtggcgc gcacggtgaa gttgaagtcg
     1981 ccggtggtgg tgggcgtgcc ggtgagccag ccgttggtgg cgaggtccaa accgccgggc
     2041 agtgcgccgc taaataccag gaagagcgga tcgccaatgg cttcgatttg ttgtgaatag
     2101 ggttcgccaa gggaaccgaa cggcaacggg ctatccgtca cgaaaatcgg cgggccggag
     2161 atgtgcaggc tgtaggactt cgaagccgtg ccgaccatgt tggtggcgta gaggatgaag
     2221 ttggcggttt cgaccttcac cggcgtaccg gtgatttggc cttccagcgt gatgcccaag
     2281 ccttccggga aggcgccgga gtataggccg atgaaattcg ggtagttggc gacctcgata
     2341 tcgcacaagt atggctgccc caccacgcca tccggcagcg gactgtcggt gatgaattcc
     2401 ggttcatcgg ccggggcgtt gatggtgatg ttatacaccc ggttgctggc gccggcttgg
     2461 ttcgtggcgc ggacggtgaa gaagagcgct tccacctgcg tcgggatgcc gtgcaagaag
     2521 ccgtcgtctt ccaaggtcaa gccagcgggc aactggcctt cgccggatac nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccgcgggat cggctgccac cagcagttgg
     2701 ttggtgtaag gcttgttttg gtaggcatcg ggcaacgtgg tggcgaggaa gcgcggcggc
     2761 atgaccgggc acagggccaa gccgtgggat acgccggcag cgatggccgt cacgccggta
     2821 gccgcggcaa ggggaatctg gtcctggccg tcgaagtatg cgccccagtg aacgatggag
     2881 ccatcggttt ttagcgcata gctaaacagg tcgccagcag cgatggccgt cacgtcgctt
     2941 tgcgcggcga ggggaacatc ggtggcatcg tcatgtggcg cgccccaggc aatcactttg
     3001 ccgttcttca aggcgagggc gtgccagcgg ccggcggcaa tggcgctcac gccactgagc
     3061 gcctccgtgg gaatatcccg gatgccccaa gccaactggt tggtggccga aataccaaag
     3121 acttgcaccc cgccatcctt gagtgccatg ctgtaaaagc cgccggcggc gatggccgtg
     3181 acgccgttgg tcaacgaaac cggaacattg cactggccgt aagaattgga tccccacgca
     3241 ataacgctgc cattcttgag caccagtgcg tgccattcgc cggctgaaat agcgctgacg
     3301 ccactgacgg cattcgccgg gatattggag gacagtactg gcgaacccca caccacaata
     3361 tcgccggaac tttttagcgc catgctgaag gacgagccgg cggacacttt cgtcacaccg
     3421 ctttgcgcgg ctactggaac gttggtttga ccgaagtcgt ttgtgcccca cgcccacacc
     3481 cggccatcct tgacggccaa agaatggtcg tagccagcgg cgatggccga agccccgctc
     3541 atgacattgg tgggcaccgg gaaagccttg aaggccgagt tattgcccca ccccagtgga
     3601 tgggttgccg cggtcgcggt gcccgcaaac cacaggcctc ccaacagaat agcaacaaac
     3661 cacgcgcgca atgacggcaa ggatttcatt attagattat tatgatctat cgcccctgat
     3721 tccgcaagtg catttggcgc aatttcgggc agatgttggc cggtcggcgt caggcgcttg
     3781 catctgccgg ccaagtgacc tactctgcgg ccatgaggaa gatcaaattg gttattttgg
     3841 atgcccacgc tacgaatccc ggagacctgt cttgggagcc gatagaagcg ctggccgatt
     3901 gcaccatata cccgcggacc ccgccggcgg aagtgctagc gcgcgtgggc gatgcagagg
     3961 ccgtcatgct gaacaaaggc gtgctatcgg ctgagacgat ccgccgcgcc cccaatctgc
     4021 gctacgtggg gattttggcc accgggtgga acaccattga cctgccggcg gccaaggagc
     4081 gcggcttaac ggtgagcaac gtgccgggct acagcacgga ctcggtggcg cagcatacct
     4141 ttgccctgtt gctggagctg accaaccagg ttgggctcca tagcgtggat gcacgttccg
     4201 gcggctgggc gcggaatccg gactattgct accggctcag cccgcaaatc gagttggccg
     4261 ggttgacgtt gggtatcatt gggttcggac aaattggcca ggcggtggca cgcattgggc
     4321 aggcgttcgg catgaccgtg ctggccaatc ggcgcgacca aacgcagcgg ctcccgccgg
     4381 gggtgcgcta cgccagcgtg gaagaaattt ttgcgcaaag cgatgtggtt tccctgcatt
     4441 gccccctgac tccggaaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4561 ccttgccaaa cgggtcggaa ttgaccagtt cgggttgctc caaaagcgct tcattaactt
     4621 cagtgatggt gccattgagc ggcgcataga catcgttggc ggctttgacg gattcaacca
     4681 cggcgacttc gtcatccgcc gcaacttctt cccccacttc cggcaattcc acaaaggtca
     4741 attcggacat ttgttcctga gcgtaatccg taatgcccac caccaccacg gagccttcct
     4801 ctcgcaccca gcaatgggcg gccgtgtagc gcaattcacc tgacgacata ttcacctcca
     4861 ctttcggaaa tgcccgcttt taccttccgt gcggtgaaca cgcaagcttt cattttcaac
     4921 agcaacggcg cggccccgaa aggccgcgcc gtgccgggcc ggcggtgccg gcagaggtcg
     4981 ggctcaagcg ccgctgagac cgccgatgat cgagaccagc ggcatgaaca gcgcaatgac
     5041 gatggtgccc accacgaccg cgaggaagat gatcagcagc ggctcgatga tggaggtcat
     5101 ggcggcgacg gcgttgtcca cgtcgtcgtc gtaggaatcg gcaatcttca tcagcatgtc
     5161 gggcagcgcg ccggtttctt cgccgacttc caccatgctg acgaccattg gcgggaacac
     5221 cttggatgcc gcgatgggcg gggccatgtt ttcaccttcc ttgacggcgt catggatcag
     5281 cgtcaccgca cgggaaatca cttcgttgcc gacggtttcc ttgacgatgt tcagcgcttg
     5341 cagcacgggc acgccagcgg acatcagggt gccgagcgtg cgggtgaaac gggagatgga
     5401 gttcttgctg atcaaggtgc caaacacggg cgcgtgcagc ttgaacttgt caatgaggta
     5461 gcgcccctgc gtggttttgg ccagcagttt gacaatcacc acgatggcga caatcacgcc
     5521 taacaccacc ggaaagcgtt ggaccatggt tgagctggcg ctcatgacca tctgggtcag
     5581 catgggcatt cccttgccgc ccatcaggtc ggcaaagata tccgcaaact tgggcacaat
     5641 gtaggtcatc aggaaagtca gaatggacag cgccatcacc agcaccacga tgggataggt
     5701 catggcgctc ttgaccttgc ctttgatttt ttcagccttt tcctggaact cggccaaacg
     5761 cgccaagacc acttcgagca cgccgccgag ttcaccggct ttgaccatgt tgacaaacag
     5821 gcggtcaaaa atcttcgggt gcgcggcgag ggcttcggca aacgtgctgc cggattcaat
     5881 gctttccgcc atctggacga cggcatcgcg cagggccggg ttcttttcct ggcgttgcaa
     5941 gacgcgcagc gcgcgcatga gcggcaaacc ggcgctgacc aaggtggaca tctgccgggt
     6001 gaacgtggtc agttgcttgg acttgacccc ctgcaaaaac ttcggcaggc gcaattccat
     6061 ttggagggcg ctcttgccct tgccgccgct ccggcgcggg gcaacggcgc cgccgccggc
     6121 cagctcttcg accttggtgg ggaaaagctg cttttcgcgg atccgggcca cggcctgcga
     6181 ctggctgtcc gactcgattt cgccatgcac ttccttgccc ttgggatcca acgccgtgaa
     6241 tttgtagcgc gccatgttta ctctcccttt attctgaact tgttgcggcc cgtgagccgg
     6301 ccttgcttac gtatacttca acacttcttc caccgtggta tagccgtcga ggatgcagcg
     6361 gatgccatcc tcgcgcagcg tgcgcattcc caactcaacc gccttgtcgc ggatgaccag
     6421 cgtgggtttg cgcttgttaa tcaaatcgcg gatcgggtcc ttgacgcgca gatactcgta
     6481 aatgccgcgc cgccccttgt agccggtttg attgcaatag gagcagcccg cgccgtaata
     6541 gaacgggcgg ccacccacct gcttctcggt catgcccagt tgttgcaaga tttccggcgt
     6601 gggctgatag gccgttttgc agttgttgca gatggtgcgc acaaggcgct ggcccagcac
     6661 cgcctccagc gtggaggcaa tcaggaaagg ctccacgccc atgtcaatca gacgggtgat
     6721 ggcgccggcg gcatcgttgg tgtgcagcgt gctgaacacc aagtggccgg tcagcgacgc
     6781 ctgaatggcg atttccgacg tgtcaatgtc gcgaatttca ccgaccatga taatgtcggg
     6841 atcctgacgc aggaaggcgc gtagcacgcg ggagaaggtc acgccaatgg cttcatggat
     6901 cggcacttgg ataatgccgt caatgtcata ttccaccggc tcttcggcgg taagcagttt
     6961 gttctcgatg gtgttcagcc ggcgcagcgc ggaatagagc gtggtggttt tgccggaacc
     7021 ggtcggaccg gtcaccacca tgatgccgtt gggcttgtca atgtcctcgc agaaatactg
     7081 gtagatgtcg tccggcattc caatattttc aaggtcgagg gacaccacgc tgcggtcgag
     7141 tacgcgcagc accacgcttt cgccaaactg ggtgggcagg gtggacacac ggaagtcaat
     7201 catgcggccg gccaccggca attggatgcg cccgtcctgc gggatgcgac gctcggcaat
     7261 gttcaacccg gaaatgacct tgaggcgcga tgtcaccggc aaggccaggt gccgcggcgg
     7321 cggcgccatt tcatagagcg cgccgtccac gcggtaccga attttgaatt cgtcttcaaa
     7381 cggctcgaag tggatgtcgg acgcgcggtc ctgcaccgct tgatacagca ccaggtttac
     7441 aaaacgcacc acgggccggg agttggccgc ggcctccatg gaggcaatgt catcgccacg
     7501 cgcgccgctt tccagcagat cccccgcccc ttccagttcg ctctcgaggc tggaaagcat
     7561 gtccttcatg ctctcgtctt cgctgccgta atagcgcgaa agggcgttgg tgatggccgc
     7621 gggctccgtc acggcgaatt ggatgtcctt gctgagcaca aaccggattt catcggtggt
     7681 ttccggcgtg accagattgg acaccgcgag cgtaatcgag ttgccatcgg cggccaccgg
     7741 catgatgctg tacatgcggg ccacgctgcc cggaaccgtc tggatgattt ccaccgccac
     7801 ggacatgtgc tccaggtcca cgcgctccgc ccccatcagt tgggcgatga tgtccagtgc
     7861 ctgatcttcc gtgaccagcc ccatttcctg cacgatgtcc ttgggcggtt tgcctgaccg
     7921 cgcgtgctca tccatgatct ccgccgcctg ggtgtcggtc aacaatccgg tgcgcaccag
     7981 ttcttccaaa aacatatcgg aatattcggt catgttcctg ccctgcctac ttttgattgg
     8041 tccggatttg ctgcaagact tcttcggtgt cctgcgcctt ggtcaccagc tcctcatacg
     8101 caatcttgcc gccacgccac agttcgatca aatgcgagtc cagcgtctgc atgccccact
     8161 tcgccccggt ttgcaggtcc gaggtgatgc ggaacgtctt gttgtcgcgg atgagcgagc
     8221 ggatggacgg cgtcgcaatc atgatttcaa acgcagcgat gcggccgggc tggtccttgc
     8281 gcggcatcag gatttgcgaa atcaccgcca cgatgctggt ggaaagctgc gcgcggattt
     8341 gctcctgctg gttggtgggg aacgcatcca cgatgcggtc caccgtgcgc gcggcgccgg
     8401 tggtgtgcag cgtggcgaac accaggtggc cggtttctgc cgcggtgatg gcggcctcca
     8461 tcgtctccaa gtcgcgcatt tcgccgacca gaatcacatc cgggtcctgg cgcagcgccc
     8521 ggcgcaacgc ctctttgaag gtcggcacat ccacgccgat ttcccgctgc gtgaccacgg
     8581 agcgctggtg ggtgtggtaa tactcaatgg ggtcttccac cgtgatgatg tggcaatcgc
     8641 gctcttgatt gatgatgttg agcatgctgg ccagcgtggt ggttttgccg gagcccgtcg
     8701 gacccgtcac cagaatcaat ccgcgcggct tgaacaacag ctccttcaac tgcgcgggca
     8761 ggccgatttc ttccagcgtc agcatcttgg tgggaatctg gcgcaggaca atgcccacat
     8821 gccccttttg gcggaaaatg ctgacgcgga agcgcgccag atcgcggaac gggaaaccga
     8881 agtccgaacc caagtcgcgt tccagcgctt cgagatggtg cgccggcgca aaactccgga
     8941 cgaggcgttc agtatcctcc gggcgcagcg gttcggcatc cagcggcgcc aaggagccat
     9001 gaatgcgcag caccggcggc agccctacgg ccaagtgcag gtcggacgcg ccttcggcga
     9061 cggccagctc cagcaggtcg tccatttgga tgtcagcggc tcgcaacgta ctcattagtc
     9121 gctatctccc atcgtcacgc gcaggacttc ctgcaccgta gtcagtccgg ccaccacttt
     9181 gcgcaagccg tcttcccgca gcgtacgcat ccccaactcg cgcgccttgg tgcgcaattc
     9241 cacggccgcc acctgatcga aaatcaggtt ccgcacctga tcgtcaattt ggaagatttc
     9301 ataaatgccg agtcggccgc ggtagccggt aaaggcgcaa tccgcgcaac ccttgccgtg
     9361 gaacaattgc gcggcggcca actgttgggc cgccggaccg agcagccgca gctcggcttc
     9421 ttccggcgta tacggctcct tgcacttgtc gcagatgcgc cgcaccaggc gctgggccat
     9481 gatggcgcgg atggacgacg ccaccaggaa cggtttcacg ccaatgtcaa tcaaacgggt
     9541 gactgcgctg ggggcatcgt tggtgtgcag cgtgctgaac accaagtgac cggtcagcga
     9601 agcattcacc gcaatttccg cggtttccat gtcgcggatt tcaccgatca tgataatgtt
     9661 gggcgcttgg cgcaggatag accgcagcgc cgcggcaaac gtcatgccga tatccgtgcg
     9721 cacctgcacc tggttgatgc cgctcatctg gtattccacc gggtcttcca cggtaatgat
     9781 cttgcggtcg ggcttgttga tggcgttcaa gcaggcatag agcgtggtgg ttttgccgga
     9841 accggtgggg ccggtcacca gaatgatgcc gtcggagagc ccgatcaggc gctcgaacac
     9901 ctgctggtcg tcggagaaga accccaattg cggcaggccc agcgcaatgc cagccttgtc
     9961 caagatacgc atgacgatgc tttcgccgtg attcgtcggg atggacgaca cgcgcaagtc
    10021 gaggtcgcgc cccatgacgt tgatctggat gcgtccgtcc tgcggcacgc gtttttccgc
    10081 aatgctcatg ttggccatga tctttacgcg gctgataatg gccgattgca agcgcttggg
    10141 cgggctgtcc acctcgtgca gcacgccgtc aatccggtaa cgtacgcgga acttcttggg
    10201 catgggttcc aagtgaatat ccgacgcgcg gttgcggaag gcttccatga tgatcaagct
    10261 aaccagcttg atgatcggcg cgtcggcttc ggtgatgttt tcatccgcca ccaactggtt
    10321 tttgacttca tccggcaggg ccaaggtgcc ttcggtgatt tccgtcagca tggattccac
    10381 cgcgccggtt gcccgcccgt agtagttggc gatggccttc tcgatttcct ctggcggtgc
    10441 caccacgcct tccacgttgg tcttgagcac gtagcgcagt ccgtcgagcg tatccacatc
    10501 cagcggatcg cccaaggcca cggtcaggac gttttcattt ttgaaaaccg ggacaacctt
    10561 gtagcgctgg gccacgtcac cgggaaccat gtcgagaact tcgcggggaa tatccaaccc
    10621 ggtcagggtg atggtttcca tgccgaactg ggcggccagc gccttgagga catcgagctt
    10681 gtccaccacg ccctcgcggg ccagcacatc cacgaccccc tcgtcgtcct gggtggccag
    10741 ttggcgcgcg gtgaccgcct gctcccgcgt gatcagcccg acattctcca gtatttccaa
    10801 gatataatcg tcgttcgaag tcaaggggat gctccttcgt cattttggcc agcccgcaag
    10861 cctgccctgc ggtattgacg cacaaccagc atgttgcaac gaaaaatacc ctttcaaaac
    10921 cattcaacgc aagcatggaa tttggcgcgg ggccggcctc gtcgtcccgg cccggccatt
    10981 gtctgtccgc agccgatttt tatcatttat ctttgccccg ccccgttata catctggact
    11041 tctgttgtaa tgacctattt gctgaacacc ccccaccccg cgccccgatc atgaggcgga
    11101 aacccattgc gttgctgagt ctgggccacg ccgtgacgga ccttgtgcag ggcggtctga
    11161 ccatgctgct ggcctttttg cagccaaccc tcggcctggg ccaactgcaa gtgggcatgg
    11221 tgatgctggc gtttaatgtc agctcgtcgg tcattcaacc ggcatttggc gtgttcagcg
    11281 accgctttca tgcggcgtgg ctgattccct ccggctgttt gctggcaggc gtgggaatgg
    11341 cggcagcggg ctatatgcca gactatccgc tgttgatgtt ggccgcgttc atcagcggca
    11401 tcggggtggc ggcctatcac cccgaagcct ccaagttcgc ccgacaggcc agcggcaagc
    11461 gcaaagcctc agggatgtcg ctgttttcgg tgggcggcaa ttttggtttt gcgttgggac
    11521 cgattctggc caccggattt ttggcgctgg ccgggcgtaa cggcacaatg ggatttttgt
    11581 tgcttaacgg tctgatggcg ctcgtgctct ggcgtaaact gccggccatc acggcaacgc
    11641 cggcccatgt tgccggcacc caacagcccc cgcgccccaa aacccgcttc acgtgggcgg
    11701 tggtctatcc ggtggtaata ctcgtcttgc tgatcatcat gcgcacgtgg acgcatttcg
    11761 gcatcgtgac gttcctgccg cagtattaca tccatcatct ggattacagc aaggaacatg
    11821 ccgccgcgct cacctcgctc ttcctgtttg ccggggcatg gggcacgctg gccggcggac
    11881 ccgccgccga ccgctggggc ctgaagccgc tgattgtcgc ttcgctggtg gtgatgattc
    11941 ccctgctcta tctctttccc cgcaccagcg gggtgccggg cttgatcgtg attgcactgg
    12001 ccggctttgc gttgatcgcc acttttgcca ccaccgtggt gctgggccag gagttgctgc
    12061 ccaataatgt cggcttggcc tccggtctga cgctgggctt tggcgtcggc accggcggca
    12121 ttggcgccac cctgctgggc gcagtggccg accgctgggg cctgcccatg atcttcaact
    12181 ggatgatcgg ctttaccctc gtgggcctgc tgctggcgct gatgttgccg ggttcggcgg
    12241 ccctcgccgc acgccgcgaa cacgcccgct actaattata tttgcgaatc accgcgcgca
    12301 ccaccccgcc gatttccagc gaccgctgcg gccggatgaa ccgatacttc ggattcgccg
    12361 gctccaagcg cactcccgcc gcatcgcgca cgtagtattt cagcgtccat tcgtcatcca
    12421 cccgcgccac cacgatatca ttctggttgg gccgcggccc cttttccacc aggacaatat
    12481 cgcccggcag gatgccggca ttgatcatgg aatcaccgcg cacggtcagc atgaaggtgt
    12541 tgtcgggatt tttgacgagg tactcgtcga gcgtgatggc gtcggcctcc tgctcttcct
    12601 cttgagtcgg gaaaccggcg gccacggagc ccagcacccg gacggtgccg gtgatgggcg
    12661 gcaacagcgc aacacgaccg tgatcatcct tggtcaggca gccggcctct tccagcttca
    12721 gcagcaagcc gaaaaccgcg ttgcgggatt tataaccaaa aagttccatc atttccgcgt
    12781 aggatggcgc gcggcgttcc tgccgccaaa agtggcgcag ttggcggatt ttctcggcaa
    12841 ggcgaatgtc tctgggcatg gcgttttcct ccctcttgat cttgtcagca atataactga
    12901 acattcgttc attatgcaag ttcttttttt gatggttttt ctttccggaa ctgcctccgg
    12961 ccgttatact ccccggcgaa tgtgattccg tacatgcaac ccaccttgga gaaccgcctc
    13021 atgaaaaaat ggagctcgtt gattgcgatt gtgttgttgt tgggtttgtt ggtcatgccc
    13081 ggctgggcat ccgacacccc gtcagtcctt ggcctggtgg aaatccgcgg gctggacgcg
    13141 ctggccggcg tggttttcga tctgaccaag tccgtcggca accccatccc caaagaaatg
    13201 acatccatga ttctctattc cggcctgggc agcatgccgg gcatgggcat cacgcccgat
    13261 ggcaatgtgc gcgtggtctg gttcgaaagc gcctcccccg ccgggagctt tgtgctctat
    13321 ctgcccgtgg ataacgatgg cgccgattat ttcgcctatc tcgatcagaa cggctgggaa
    13381 aatcaggggc ggctctccga caagatcgag cactactccc ccccggagga tgccggcgtg
    13441 ctgtgggaaa atgtgtactt catccgccac ggcgcgacgc tggtggccgc cgacaatccc
    13501 ggcgatgccc gcaaaggcga atcgctcctg aacgcgctgc cccccatcct gccggtggaa
    13561 ggcgtgctgg ccatccaagt gcgccccgcg gccctgaccg atacctatgg gccgctggtg
    13621 cgtgagcaaa tggatgtcat ggtcaacgac ccggatgcgc ccgcgcaaat ggagccgttt
    13681 gccaaggaat actttgacgc ctatttgcag gtggccgcgc aagtggatga agttgtgctg
    13741 ggcgtgggcg tggcgaacga caagctcaac ctgcactacc gcgctgcccc catcgccggc
    13801 accacgctgg cgcgctggat ggacaccatt cgcgctccgg gtgcggcatc tgctgtggtg
    13861 gccctgccgg gcgcactgat cgtcagcgcc gtcaacatgg gtgacttcaa catgatcgcc
    13921 ggcccgtatc tgcgctttat ggagaaggcg ttgaaatcca tgccgcagga aatccccgag
    13981 gaattcatca cccgctacat ggcgctggaa aaggccggct acgaacagct tgtcggcgat
    14041 atcagcatgg cgctgttgcc gccgaaggcc gacctgccgc tgcgtttcgt ggaatatgcc
    14101 aacctcaagg atgtcgccgc cacccgcggc ctctttgtgg aaggtgttaa tttgttcaac
    14161 gatggcatcg cggccatgct cgccagtgtt cccgaagcca aggagagtgg cgtcaacctc
    14221 accgcgaccc tgagcacacc gcgcacctac cgcgacattc cggttgacca aatgcggatc
    14281 gccattgagt tgccggaaga agcgcaggca gagtggccgg ctttcctgcc ccggacgttc
    14341 accgcggatg tggcctggct ggccgatggc gctgtcttca gcgtcggcga tcccgcgctg
    14401 atcgagcccc tcgtggaccg cgcattggac ggcgccaccg atggcgtcac cagcctgccg
    14461 gcctggaagg cggccttccc caacccggaa accccgattg tggaagcctc gcacatcgca
    14521 ctctttgaca ccatccgcag ctttgtccaa tggctggatg cggccaccgg ggagaaccat
    14581 gccccggcca tccccgccgg ccccggcaac gtggaaagcg cctcctatct ccaggatggc
    14641 tatatgagcc aactgcgcat caatttggcg gaccttggcg ccatcggcgc aaaaatcagc
    14701 gaggcccgcg aaaaggcgat gcagcagcgc cagctctact tgggcggcga taccaacgac
    14761 tggtacgaca tggacgacac cgacgaagac gaagactctt ccgaggagac gcccgcaacc
    14821 gagtaaccgg tgcggcccac ctgcctgagt atattctgga gttttctccg gattggcgct
    14881 ggcgcgttcg gcggcggcat ggcggctctg cctgtctttg aggcggagct ggtcaaccgc
    14941 cgccgttggc tttccgcgcc ggataccgcc gagttgttcg ccatcgccca atcgggtatt
    15001 ggcgtcatca ttgtcaactt tgcggtactg gccggcctgc ggctggtggg caaacgcggc
    15061 gcgttcgtgg cggtggtggc cgtggtgctg ccggccttct tcgtcattct tgctttggcg
    15121 atgctcgtgg ccggcaactg ggaaaaccgc tggctctccg cggcgctggt tggcctgcgg
    15181 ccggccgtgg tggccatcat tgtcggcgcc gcgtggcggc tgggccgtgc caacctgcgc
    15241 acgttctggc ccattctggc ggcggcggct tgcgccgtcc ttttgttcca acgggtatgc
    15301 agtccggtgg tgttgatttt gctcggtatt gccgtcggtt tgattctcca ctggttgcgc
    15361 cgcccgcgcc ctgcaccatg atgatctatc tccagctctt cgccacgttt ttcgagatca
    15421 gcctcttcag cattggcggg ggttacaaca tgatcccgct gatgcagcac gccgtgaccg
    15481 ccaacggctg gttgagtgcg gagcgttttc tcgacattct ggcggtttcc gaaattacgc
    15541 cggggcctat cgcggtcaat ctggcaacat ttgccggcta ccacaccgcc ggcttttggg
    15601 gtgcggtggc ggccaccgtg gcggtgtgtc tgccgggcag cgtcattttg ctggtgctcg
    15661 gctcgttggc gtttcgcctg cgcgaccgcc cggcattccg cgccgccatg cgcggcctga
    15721 tgcccgcgct ggtcggcttg ttgacggcaa ccgccctgcg cctcgccacg gcgttgatcc
    15781 ctgccggcgc gcattggcca tcgctgcaag caagcgtggt attcgccgtg gcgctaacgc
    15841 ttttcctctg gaaaaacctg caccccgcgg tgctgatgct tggcgcggcc gccaccggcg
    15901 tggcgtggtc gctagtggcc tgaccctcgt cccctccgct ccgcaccccg tttcacaaac
    15961 gaaacgggcc gggagtctcg ttttccgctt cctctttggg cggcggcttg ggccggaaat
    16021 gcgggcaggt gtccgtggcc tgcacttcgc atttgtgcag cccgcaacgc tggataaacg
    16081 gattcaccac atactccgcg caatagcggc acatccggcc tttttcatct tcgcggaaaa
    16141 cctgcaccgg gcgcgggcgg tcggcggcag tgaacacctg cggctggcgg ctgccgcgga
    16201 acggcacctc ggactcgctg gcaaactcgt gggcgcacag cgcgcaggtc agtgtctcgc
    16261 ccaccttggt gaagccgtca tacttcggct tgcgcacaag ccaagcctcg cgcccgcaag
    16321 ccgggcattg gatttccacc ttggctggct cgcgcccttt catttcagaa gaaccacttg
    16381 gccagccgca ggatgccctg cttccacggg acgcggtgcg acaccccact cgcgttgcgg
    16441 aaggcgtcca agttcgccac ataccaccgg taattgcgca ccagcgcttc cttgttggaa
    16501 tacttgggcc ggaagcccag cacccgctcc gctttttcga tgctgacaaa cgaatcttcg
    16561 ctggcggttt cataaaccca cttgtagagc ggcgacaact tcagcagctc caaaaaccgc
    16621 agcgtccaaa tcatcggcgc ggccgggaag ccgcggattt ttttgccgtg gccggcatcg
    16681 tccagcaccg cctgataatc ttcgcgcatg gtggtgaaat ctttggcgcc gatgttgaac
    16741 gtgtcgttca ccacggccgc cggcagcgtc atggtcaacc agatggcttc gcagaggtct
    16801 tccacgtcaa gcaactggta gcggttgcgc ccgttgccga tcatcgggaa tccgtggccc
    16861 gtgtaggccc agtcataaaa caacgcaaac accccaaggc gctccggccc aatgaacgat
    16921 ttgggccgga tgatcggcac gcacatcccc tgctcgcgga acgcgatgca ttcttcttcg
    16981 gcggcaattt tagcgcggcc ataaggaccc acgccggtca gtttgtcggt ctccagcagc
    17041 gggtgatggt cgggaatgcc gtacaccgcc gtgctggaaa tcatgacgaa gcgcgtcacg
    17101 cccgcctgct tggcggccgc cagcagattg cgcgtgccat ccacgtccgt ggtcatgatg
    17161 tcgcgctcgg aatagagcgg cagtgcggcg gcggtatgaa tgaccatgct cacgccgtcc
    17221 attgcgcgcg ccacggctgc cacatcgcgg atgtcaccgg tgtgcagttc cacactgtct
    17281 ttttcgggat aatcaaacgg cacgatgtcc agcgagcgga tgtccgtttc gcccttggcc
    17341 cgcaagtgcc gaatcaaatt gattcccaaa aacccgctgc cgcccgtcac cagaatctta
    17401 ccgatcgcca tagtcgctct ttccattcat ctcatcacag tttggccaaa gttgcgcgca
    17461 ggactgtatc cacgtccaag tccatgaggc aagccagatc cccccggcgg catgtgcgcg
    17521 cgtggcacgg tcggcaatcg aggccctcct tgaccaccac gccgtcgcgt tgccgccacg
    17581 gcccggtgcg cgcgggatcc gtcgcgccat aaatccccac caacggcaca ccgagcgcgt
    17641 ccgcccaatg cagcggcccg gaatccaccc ccaccagcaa atccatgccc ttgagcaacc
    17701 cgcacagtcc cggcaaatcc gttttgccac acaaattccg gcatctctcg ccaatctgcc
    17761 gcgcgaggct ttcgccggct tcggtatcgc ccgcgccacc caccacataa atccggcagc
    17821 ccatttcgcg aatcatccgc tgccccagtt ccgcgaattt ttcaacgggc caatttttgg
    17881 tggcccaccg cgaaaacggc gcaaaggcca cgtgcgggcc ggggctgcca tcgccgggat
    17941 tttccgcaaa gtccaactgc agttccggca cctccgtgcc gggcgccagc aaattgacca
    18001 ccgccaacat ttccgcggtg gcgtggccgc gcgggccggc ggtcttcgcg ggttgctcgt
    18061 ggtaaaaccg cgccgcgccc tcccgcgccc aagccggccc atagcgccgc cggccgcgcg
    18121 ccaagcgcgc caccagcgcg ctcttcaaca acccttgcaa atccacgatg gcatcatagc
    18181 gctcccaacg cagctcccgc cagaacgccc aaaatccgcg tgcaaaatta cgccgcggga
    18241 acaaaatcac ccgctccacc ccgcggagtc ctgccacgag gttgccgtat tccggctgtg
    18301 tcacccaatc cactgtggcc ccggtgcgcg cttgcaacgc gcgcaccgcc ggcaaggcgt
    18361 gggcaatatc gcccaacgaa ctcaacttga tgaccagaat ccgcatctca gccggggcgg
    18421 gacaaaattt cttccacccg cttgattttg agcgccttgc cggtttctgg gtccacggtc
    18481 agtagcgcgc catgtagccg gacatcgccg ctggcgattt caaacttggt gggcatgcct
    18541 gtcaggaaac gcgtcaggat ggcctccgta gtgcggccaa tcacggaatc gcacggaccg
    18601 gtcatgccca aatccgtcat gtaggcggtg cctttggcgg tcacggcttc atcggccgtc
    18661 tggatgtgcg tatgcgtgcc cagcaccgcc gtcacgcgcc catccagaaa cttgcccatc
    18721 gccaccttct cgctggtggc ctcggcgtgc atatccacaa tgatgatctt gtgcgccgcg
    18781 ccgtgttcct tgagccacgc atccaccgcg cggaagggac aatcggccac ggtgttcatg
    18841 aaagtgcgcc ccatcagttg cacaatcgtc accggccccg ccggcgacgg caccgtgctg
    18901 acgccgtgcc ccggacagcc cggcggcagg ttcagcgcgc gcacaatgcg cggctcgcgg
    18961 tcaaagtagg ccgccgcttc tttctgatcc cacacatggt cgcccatggt cagcgcatcg
    19021 gcgccgcccg cgaacagctc ttctgccaat gccgcggtga tcccccgccc cgccgcgctg
    19081 ttttccgcgt tggccagcac gaagtccgcg cgcttttccg ccttgtagcg cgtggccacg
    19141 cgagccatgg ccatgcggcc cggcccgccc acaatatcgc ccaccaccag gattttcatg
    19201 tcacgcgctc cttgtttccc ttcaccggac atactccacg caacgcagct cccggatgac
    19261 cgtcactttg atgggccccg gagagtccac gcgcgtctct atttgctggg cgatgcgccg
    19321 cgccagttga atggcagcgg cgtcatccac tgccgtcggc tccacaaaaa tacgcagctc
    19381 gcgcccggcc tgcatggcga ggcttcgcac cacgcccggt tccgccgcgg caatttcttc
    19441 cagattcttc agccgttgca aaaattgttc cgtagtgtcg ctgcgcgcgc cggggcgcgc
    19501 ggcggtgacg gcatccgcca cagccaccaa cgcggcatag acattggccg gcgcggtttc
    19561 cacgtggtgc gtccgtacgg ccgccaccac ctccgcgctt tcgccagcgt gctgaagcaa
    19621 gtccgcgccg atctccgcgt gcggaccacc cgcctcctgc gtggccgcct tgcctaaatc
    19681 atgaaacagc cccgcccgct tggcaatgtc cgcgttcagg cccagctccg cggccagcaa
    19741 tccggccaca tgggcggttt cgcgcgcgtg ttgcagcacg ttttgcgcga aactggtgcg
    19801 gaacttcaac cgccccagcg cccgcaccac ctccccggcg gctggcgcca gattcagctc
    19861 ggtcagcgcg tcttgcccgg cctgcaaaat caagccgtcc acttctgccg ccactttgtc
    19921 caccatttcc tcaatgagcg ccggattgat ccggccatct gccaccagcc gctccaatgc
    19981 gaccttggcc acttcgcgcc ggatgggatc aaacgacgac agcaccacca cttccggcga
    20041 atcatcaata atgaccgaca ctcccgtcgc cagttccagc gcgcggatat tgcgtccttc
    20101 gcggccgatg atgcgcccct tcatgctttc atccggcagg cgcaccgcca cggtggtaat
    20161 ctctgaaaca tggtccactg ccacgcgcgg caggacatcc atcaaaagcc gccgggcatc
    20221 atgtgcggcc tcggcgcgtg cggcggcctg cgccttgcgc agccagtcgg cggtttccgc
    20281 cgcgatttct tcctgcaagc cggcgcgcaa ttccgcacgg gcttggtcgg cagtcaggtg
    20341 ggcggcggtt tccagccgct gctgggcggt ggccatggtc gcggccagtt tctcctgctc
    20401 ggcggttagc gtggcgcgcg cggcttccaa ttgctgggcg cggacatcca acgcctgcgc
    20461 ccgttcttcc aaccgctgct cctcacgctc caaacgctca tcctggcgcg acaaccggtc
    20521 cgccgcggcc gacagcacca gttgccgctg ctgcaaatcg ctttcggcct gctcgcgttt
    20581 gcgcaacagc tcatcgcgcg cggccagttg cgcctccttg cggatgactt cggcatcgcg
    20641 ctgcgcggct tgcaggattt ccgccgcgcc gcgttgcgcg ctacggctcc gcagccgcac
    20701 caccagcatg tgcagcccca cgccaatcag cgtgaagcct gccgccgtca gcaccggcca
    20761 gaaccattcc caaaagacgc tcatctcatc ccgtcagccg tgccagtttt tcttccgcgc
    20821 cggccatgaa ccgcacctgt ttttccgtca cgatcaaatc cattcgcgcg tcatgggcat
    20881 ccatcggcac ttgcgtcacc aggcgcgcct cgtcgcagag ccccacccgc agcgcgtcgc
    20941 ttcccgccag caggcggtca tagtgcccgg tgccgtgccc caagcgcccg ccggcgcgat
    21001 caaacgccac gccgggcaca acaataatcc gcagctccgc ggcggcagcg ggcacccgca
    21061 gcgccggctc cataatgccg tgcgcgccag ccacccagcg ggtcgtggca tccacccaac
    21121 tccagacgta gccgttcttt tcacgccgcg gcaccgccac ctgcgcgccg cgcgccaaca
    21181 aatccgccag cagcaaatcg gtcgccggct cggtggccac cgcgcggtac aggcccactt
    21241 gaaaacccgc cacccaccag tcttgcgcca gcagcatctt ttggatagcc gccgaggccg
    21301 ccggcgcgac ccgtcggttc ttgaactgtg cgcgcagttt ggttttcatt ttagcaatcc
    21361 tcgcaagtcg aatcccgccg gcgggcgggt cggccgcccc ttggcattca ccacgcacca
    21421 gtgcacggtg gcttcggcaa tcacatcctc accgcggcga atcacctgct ggaacgcgcc
    21481 tccaacccgg cgcggttcgc ccgcttcggt ttcaatggtc agctcgtcat cggcataggc
    21541 cggctgacgg tacgccagtt ccagccgcgc aatcaccgtg aaaaatcccg ctgcaatcag
    21601 ccctttgtag tcaaacccca cggcatgcaa aaactgcatc ctggcgtgtt ccaaatagtt
    21661 aacgtacacc gcgtggttca cgtggccata cgaatcgcac tcgtagggcc gcacagtaag
    21721 ctggatcaca tgcttcatat acgcgccgca ccatacaccc ccccgccccg ctggtgcaag
    21781 ccatccaccc gccccccgcc cgtcgccgcg tcaaccgcgg ttcaattcat aaatccgcgc
    21841 cagcccgtgc agtgtcagat cgggatacac cggaatcttc agcccggcgg tggccagcac
    21901 cttgcgcgca tagccgcccg tggcgcaaac gatgggttct tttttgccgc aggccttgct
    21961 caactgccac aggatttccg tcaccatgcc gcggaaaccc cagcgcgcgc tcaagcgcat
    22021 ggcttcctcg gtgttgcgtc cggtgggcag cttgatgccg cccggtttca gcttgggcaa
    22081 ctgcgcggcg cgctggccca tggcggtgaa ccacatgcca aagcccggcg cgatgacccc
    22141 gcccacgaag ccgcgccgcg ccaacaccag attgaacgtg gtggcggtgc cgaagtcgca
    22201 cacgatgcac ggcgtgccgc acaactccgc gccggccact gcgttggcca gccggtcgtg
    22261 accggtttgg tcggggcgct tcaaatccag attgatgccg tagtccaagt cggtatccag
    22321 ccacagcacc ggcacacgcg ggaatgtctg gcgcacgagg cgcgtccacg tcgtgttcag
    22381 cttgggcacg acggagccga ggcagatgcc gtcgggaatt tcgccgttgg ccaccgtttc
    22441 cagaatgacc gggctgacgc tttcggtgcg cgccgcgcgg cgaatcttgc cgttggaata
    22501 ccagccggcg gccgttcgtg agttgccaat gttgactgcc aggattttca tagttccggt
    22561 tcatcctccc gggcgccggc ggccaaccag cgggcccgca catccgccaa gggagcttga
    22621 acaaccctcc gcaatgattc caccaaaagt tcaagcatct cgtccacaga aatctgacag
    22681 ccctgcaacc ggcaggacgt cacgcggccc tgcaactccg ccggaaaatc accgcgcgct
    22741 tgtgccacat tgatgccaat ccccaccacc gcaaactcga tgcgcccggc gcccagccgc
    22801 ggctccacca gtacgccgca gattttttgg ccgcgtgcct gaacatcgtt gggccacttc
    22861 acgcgcagtt ccggcacgcc cgccgcatgc agcgcatcat gcgccgccat tgcgcccaga
    22921 attgccaacc cgggcgcctg tgccgccggc caatccgggc gcagaatgaa cgacgcatag
    22981 acccccgccg ccggcagcga ttgccatggc cgcgcccgac gcccgcgccc gcggctttgc
    23041 tgcgcggcca ccacaagcgc gccggcttcc gcccccgcca gcgcccaatc gcgggcgaca
    23101 tcgttggtgg attccgtagt cgccagccag cgccttggat agccccacgg catggccgcg
    23161 ctactcgaag tccaaaccca aatccgccgc gcgcgaagaa tgcgtcaacg cgccgacaga
    23221 gatggccgtc acgcccgcgc gcgcatattc gcgcacattt tccttggtga tgccgccgga
    23281 tgcttccgtc ttgcaggcgc cgccgcacaa ttccacggcc tcggccactt gcgcgggggt
    23341 catgttgtcc agcagcaccc agtctggctg gccgggcagc gcttcgcgca attgctccag
    23401 tgagtccact tcaatttcaa ttttcaggtt ggggtacgcg cggcgcgcgg cgcgcacgca
    23461 atccgccagc gagccctgtt gcgtgcgccg ccagaaggcc acgtggttat ctttgattaa
    23521 caccgcgtcg tgcaacccct gccggtggtt cgtgccgccg ccgcattgta ccgcgtactt
    23581 gtccagcttg cgcaggccgg gcacagtttt gcgggtgtcg agaatcaagg tgccatagtc
    23641 cgccacaatc cgcacgaggc actccgtggc ggtggcgatg cccgtcatgc gctgcgcaaa
    23701 gttcagcgcg gtgcgctcgg cggtcaacag cgcgcgcgcc ggcccggcca ccgtgagcag
    23761 cgcggttccc gccgccacac attcgccgtc ggggcacaga atctccactt gcagcgccgg
    23821 gtccgcctgc cggaataccg cagcggcaat ctccatgccg gccaccacca cctcgtggcg
    23881 ggccaccaac cgcgcgcgtg cggtttcccc ggcgcccacc agcgccaacg tggtggcatc
    23941 gccagaaccg acatcctccg ccaaggcacg cgcgatcaac tcacgcactt ccgcatcctg
    24001 catcaacgtg ttgtctggat tcatgggatt gtctcctttt ttgcgctcat gatttgcggc
    24061 ggcgcgtgct tgacggacgt ttgccgggca accgcttgcc cggatgatag acaatggctt
    24121 tgcgcttgcc aatttgcacc gtgcccttga acggattttc ggttttcttc aattcataga
    24181 tttggtcgcg cagcagcgcg gcgcgttcga actccatggc ctcggcggcc tcgaacatct
    24241 cgcgctccat ttccgcgatg gcgcgcacca cgtcataatc ggcgccggcc tcgtggacgg
    24301 caaactcttc ggcttcgcgg gccttctcgg cctgcatgag gctttcactg atggcctttt
    24361 caattttgcg cggcgtgatg ccatgcgccc ggttgtattc ctcctgctgg cggcggcgct
    24421 cgctggtcag gtcaaccatc cgctgcatgg aatccgtgac ttggtcggcg tagaggatca
    24481 cgcggccttg caggtggcgc gcggcgcggc cggcagtttg gatgagcgat gtttcgctgc
    24541 gcaggaagcc ttccttgtcg gcatccagca cggccaccag cgccacttcc ggcaggtcga
    24601 gaccctcgcg cagcaggttg atgccgatga ggcaatcgaa gtccgccttg cgcagcccgc
    24661 gcaggatgtc cactcggtcg agcgcagtga tgtccgagtg caggtatttg cagcggatgc
    24721 cgatgttttg caaataaacg gacaaatcct cggccgtctt tttggtcagg gtggtcacca
    24781 atacgcggtc cttccgctcg gcgcattggc ggacttcttc aatcaggtcg tccacttggt
    24841 tgcgcagcgg gcgcacctcc accagcgggt cgaggatgcc agtggggcgg ataatctgcg
    24901 gcacgggggt gccggccagt tggaactccg tcgggcccgg cgtggccgag gtgaacagga
    24961 cttggttcgt cagccccatg aattcgtcat agctcatcgg ccggttgtcg agcgcggagg
    25021 gcagccggaa gccgtgctcc accagcgtgc gcttgcgggc ctggtcgccg ttaaacatgc
    25081 cgcggatttg cggcagcgtg acgtgcgact catcaatcac cgtcaaaaag tcgcgcggga
    25141 aaaagtccat cagcgtgtag ggccgcgagc cgcgcggacg gccggaaagc gggccggaat
    25201 aattttcgat gccgctgcaa tagccgacct cgcgcagcat ttccatgtcg aaggtggtgc
    25261 gcatccgcag gcgctgggct tcgaggagct tgttctgggc ttccaactcc gccacccgcg
    25321 cctccatttc cgccagtacg cgcgtgacgg ccggggcgat tttttccgcg ggcatcacga
    25381 agtgcttggc cggcgaaatc gtggtgtact cgaaatgctg gagggttttg ccggtcagtg
    25441 gatcaatgcg acgcaggctt tcgatgcggt cgccgaaaaa ctccacgcgc acgccgtcct
    25501 ggctgtagcc gggaaaaatg tcgagcgtgt cgccgcgcgc gcgaaacgtg cccgggagcg
    25561 tggaaatatc gttgcgggtg tattgcacat ccaccagctt gcgcatgatg tcctcgcgcg
    25621 cgaactcttc gcccgcgcgc agggtcacca gcatcccgcg gtaatcctcg ggcgagccca
    25681 ggccgtagat gcacgagaca gaggccacga tgaccacatc ctcgcggttg atcagcgcat
    25741 ccgtcgccgc gagacgcagg cgttcgatct cctcgttgat gctcgcatcc ttctcaatat
    25801 aggtgtccgt ttgcggaatg taggcttcgg gctggtagta gtcgtaatag ctcacgaaat
    25861 actccacagc gttgcgcggg aaaaattcct tgagctcggc gtagagttgc gcggccagcg
    25921 ttttgttgtg cgagatcacc agcgtgggcc ggttcagttg cgcaatcacg ttggccatcg
    25981 tgaacgtctt gccggagccg gtcaccccct ccagcgtgcg atagtgcgcc ccctcccgaa
    26041 agccggccac cagcgcggcg atggcctccg gctggtcgcc ggtgggctga aagtccgcta
    26101 ccagttcaaa tccggccacg atcgcccgtc ggggaactca gccccagcgc tccttcatca
    26161 cagtttcgcc gaggggccgg cgcgagcgcg ggcgcggatt gggctccacg tcggggaagc
    26221 ccagcggcgt catgcccacc acttccacgc catccggcag gcccagcgtg gtgcgtaccg
    26281 ccgccggatc gaacgagccc acccagcacg tacccaaccc ggcttccgcc gcggccagca
    26341 tcaggtggtc catcgccagc gcgccatcca ccagcgcata atttttgccg tcataggagc
    26401 gcgtccaagc ctcgccgggc accacgcaca ccacaatgat caccggcgct ttccagaacc
    26461 attcgcgcgc gtaggccgcg cccagcgcgc gccgcgtggt ttcgttgcgc accacaatga
    26521 agcgccaggg ctgcttgttg caggccgacg gcgccaaccg ccccgcctcc agcaccgttt
    26581 gcagcagcgc ttccggcacg ggctccgcct tgtaggcgcg cacactgcgc cgcgtggtgg
    26641 ccaaatcgtg aaacgccggc gacacggcct acctcttggt ggtttgcaac cgcccgccaa
    26701 agttgccggg ctggagcgga tcggtggaat aaccgtcaat gcgcaccgtg gccgagtggc
    26761 cggcaaactc gccggcggcg atcaggtgcg cactcgggaa cttggccgtg gcgctggttt
    26821 ccttgacgat ggtgaacacg ttgccgttca ccgggccgct gagcacttcc gcgccccaca
    26881 gcccgacaat ctgcgcggtg ccgttggtca catagatatt cacgagcccc gcgtcgcaga
    26941 ccggcgcggg cgtcgggttg tttgtcgtgg ccgccgcgtg gaagcagatg tcgtaactgc
    27001 cgccatagtt gccggccacg gccgggttgg gctcctcgcc gctgctgctg ccgctgttat
    27061 agctggcaat tgccagcgcc ccccccaccg ccgccgcgcc acccacgatg attgccgccg
    27121 gcagcagata cttggatttg ccggacttcg gcggggcggc cggcggttgc gccgtggccg
    27181 ggccagccat ttgctgctgc gcctgttgtg ccacggtgct cgcggcggga atcgcctccg
    27241 gcgccacgcc ggactcgacc accttcaccg tctgccattc cgtttcgcgc gattcgccat
    27301 ccgcgttttc ggcctgaata taatagaaca actgcgtgcc gggaccgatc atgtggcccg
    27361 gaatcgtgcc ataccaggtg cccgcgcccg aactggtcaa cggcacgcgg aacggcgtca
    27421 tcccgcgcga cgccgcataa aacagtgata ccccctccac ccgcgccgtc gcgtcgcgga
    27481 cggtggcccg cacgccgagc ggctggccct tcaccgccac cttgatcgga tcgtgcacaa
    27541 tcgccggcgc cccgcacact ggcgcggcgg ccatcgccaa ggccagcaag gccgcccaac
    27601 atctggttgt ccaagtcata aaaactccac ttttccatgc cgtggaaaaa agttttccac
    27661 gctgtggaaa aaatatttcc acactgtgga aaacctatac ccgattcctc cccgcatggg
    27721 aagaggccag aagaagcagg ttccgtcagg cttgttctgg ttcgggtttg cgcgtacgcc
    27781 gcgcggatgt tttggccttg tttgctgctt tcttaacacg ataactgacc ttgggttctc
    27841 caacggcggg atactgatcc aaccaagcaa ggtccttgcc aatgaccaat tcggacatag
    27901 tcgcattgtg cgtgttggtc atcgggatca atcgcatttg aaagccatgt gcgcgggcca
    27961 ttatcttgac ttcttcggca ttgtcatacg tcatcaaaaa ctcgcccgca attgtttcac
    28021 agatcgaaaa aagacggtga tgatccagct cgcaatgccg ataaagccgt ttgcctgctt
    28081 ttttcccgcc agcggtatag gggggatcaa taaaaaaggc ggccctttga tccgcagcat
    28141 gccgctgaat aactgcaagg ccatcctctt gccgaaactc catacggcga gctatcagat
    28201 tcatattggt gaggcggcgc gcaagagttg ctgggtacca ccgcgattta atacccttgc
    28261 cattttcacc atattttaaa aatccggagc ctgcggccaa aataccccca tgatacgtgc
    28321 gatttttaag aatggtttga aatgcttttt ccttgctcga tcttggcgtg cgggagattt
    28381 ccctcaccac tgagtctttg gtcaggtcaa actccatgat tcgccgggcc aaccaatcgg
    28441 cctcgccgtt gaccaccgcc cgccaaaccg cagcaatatc gtcatcaatt tccaccataa
    28501 cagcacgctc aaccaagttc tcaaagagcg ccgttaagct gataatgccc cccccaacaa
    28561 atggctcaac aagatactgc ggtttgggga aaatactgct tatccaggac cgaaacgtgg
    28621 gcacaaacca cgttttcccc cccggataac ggaatggact cctttgcggg atggaagcaa
    28681 cattaatcgg cttgggagct tcaagcccat cgccaagttc tgggaacaga agtatttggg
    28741 cttctttgcg cattaaaagg gacgttcaat tgtttgattt gcaggaggcg tttccagttt
    28801 ttcatcgagc cgttcctgga gctgtttgac aaatgattct acttttcctg gcaccggtgt
    28861 cgtaatcgag cggagagccg gtgtgaattc ggtaaaaact tcgtctactt tagtgaggcg
    28921 gtatcggggt tggccagagt gttcgctgag ctgcaaatcg taaatgaacc aagcaatgtc
    28981 tgctttggat ttagctgttg tagtaaggcg gggcaaggta tcaaagaaac tcttattcag
    29041 cgcgaccgcg cactttttgc cccacgaatt caagatgcca cctttgaaga gcaactgtgg
    29101 agccaaacgc tttcgggatg atgacaaaaa atccgggcgg ggataattcg gctgatctga
    29161 ccagtccatc tctcccctgc gctttgggtc tttcacgtaa tactcgaagg gagccctgac
    29221 atttcctgat atgtaaaccg cctgaacctc caatgcgccg aaatcaagta ccttgccttc
    29281 ggaatcataa gacaccaaaa ccatgtcaat gttcccggca gatttgccat acgcatcgtt
    29341 gagtctgact tccattaagg aactccacgc tacgtcatcc gggaaaaaga aagaggccgc
    29401 atcgtctgtg atcagccaat cttcgcggaa acgaatgggg caggtaatgg cgggttgacc
    29461 gtcatgtaaa acactgcaaa ccccgagcgg attttttgcc ttgtcttttg tgcagtttgg
    29521 aaccgagttg ttaaacggac aaagacgccg caaacgatag cgattggcac tggctgattg
    29581 gtcagttatc agatgtccaa atacttctgc caacggctga tgtttttgct tcatttctat
    29641 gactgcatct ctttctttag ccttttgagt cctgcgggca atctctgttc ccattattta
    29701 agcgccgcgc ggccgtcacc agctatagtt cacggtgtag gtcagcacgg tttcgccgtc
    29761 ggccagcacc ggcacgcgga attcgatggt gtgtgaatca aagcgcgtcc actccgcgct
    29821 ggcggcgctg atcttccagt tggcggcgcg gtagagcggc tcaatcacgc ggatttccac
    29881 ggcttccttc tccttgcgat tgcgcagctt gatttcgatg gtttcgctca tccgctgggc
    29941 ggtggtgtcc acggagaaat cagtttgctt gcgctcgccc accagatcaa acgcgctgcc
    30001 catttcgagt ttgagcttct cgttcttggg caggtggtcc agctcgtttt cgccgatgaa
    30061 ctcgcgccgg ccatccacgt cggtccggta caggcgcatc cggcccttgg gtagcggcac
    30121 gccaagctgg ttattctcgc ggttttcaat ctcgatgcgc acgcccacct tcttgatggc
    30181 ggtttggccg taatcggggt cactgttgag ccggcgccaa tatcgggcat ccaccagcgg
    30241 attataaaca tagtagcggg tgcccttcac gccgctggcg cgcaaaaact ccacctgctt
    30301 gagctcgttg ttccgcacct gcaccggccg cggcagagtg taaagatgga attcatcaaa
    30361 cgaacgttct tccggcatgg catcagcgac tgccatctcc ataacaacat ccactccgaa
    30421 cctgccatca aaaccccggt ccagtttctg cacgtcgccg gcaatcagtt tgacggtcgt
    30481 atcggtgaag tctttgccag tctcattctg caatgaaatc cagccggaaa agtcaaaggt
    30541 gtcgccgcct tccgcgggca tcaccagatt atacgtggcc tcccagctca tgccttcggt
    30601 gacataggcc acctccaccg ggcactcccc cgcccgctct gccgccaact gccacaccag
    30661 cgagggcttc agaaatgctg cggggtccag cccctcgaac accggctggc cgggcagcga
    30721 aaaccgcacc gcgccatcgg tttcgatgat tggctgctcg ctgcggccac ccgggatgta
    30781 gccgctgcgg atcaccttgc cgcggtgttc cttgatggtg ccatccctcc gcttttcttc
    30841 aaagcgtacg ttctggcctt ctagttggta aaggagcaag ccttccgtca gcgggtcgtt
    30901 gacaaaactc tgctcgagaa tgcgcacgcc gaagggtttc ttgcccagct cgcgcagcat
    30961 gacggaatcc ggctccaact ggcgcgacat atccgtcacg cgcacggtgg aaacaccctt
    31021 ggtcaattgc agcgtgcggg tttccttcac cgtggcgaat ccgtcgttgt aaatggtcac
    31081 ctgcggcccc gtggcccacg ccgccatcgg cagcaacaac agcaagggaa tcagtttttt
    31141 catggcctct ccttggcggg cgggtgtcac cagctatagt tcacggtgta ggtcagcacg
    31201 gtctcgccat cggccggcac cggcacacgg aattcgatgg tgtgcgaatc aaagcgcgtc
    31261 cactccgcgc tggcggcgct gatcttccag ttggccgaac gatagagcgg ctcaatcacg
    31321 cggatttcca cggcatcctt ctccttgcga ttgcgcagct tgatttcgat ggtttcgctc
    31381 atccgcttgg cggtggtgtc cacggagaaa tcagtttgct tgcgctcgcc caccagatca
    31441 aacgcgctgc ccatttcgag tttgagtttc tcgttcttgg gcaggtggtc gagctggttt
    31501 tcgccggtga actcgcgccg gccatccacg tcggtccggt atagccgcat ccggcccttg
    31561 ggcagcggca cgccaagctg gttatcctcg cggttttcaa tctcaatgcg cacgccgact
    31621 ttcttgctgg cggtttggcc gtagtcagca tcggtgttgg cgctgcggga gtaaccataa
    31681 cccaccagcg gattatagac ataatagcgg gtgcccttca cgccgctggc gcgcaaaaac
    31741 tccacctgct tgagttcgtt gttccgcact tgcaccggcc gcggcagggt gtagagatgg
    31801 aattcgtcaa acgcgcgttc ttccggcatg gcggcgggcg cgggtctggc tcccatcagc
    31861 actaacggct ggcgatattc cggacgccgg gtcaccttct gcacgtcgcc ggcaatcagt
    31921 ttgaccgttg tatcggcaaa atccttgccg gtgctgttgc gcagcgaaat ccagccggag
    31981 aagtcgaagg tgtcgccgcc ttccgcgggc atcaccagat tatacgtggc ttcccagctc
    32041 atgccggcag tgacataggc cacctccacc gggcactccc ctgcccgttc cgccgccaac
    32101 tcccacacca gcgagggctt caagaatgcg gtggggtcca gcccctcgaa cacgggctgg
    32161 ccgggcaacg aaaaccgcac cgcgccatcg gtttcgatga ttggttgctc cgagccgccg
    32221 cccgggatgt agccgctgcg gatcaccttg ccaaggtgtt ccttgatggt gccgtccctc
    32281 cgcttttctt caaagcgcac gttctggcct tccatctggt agagcatcaa cccctccgtc
    32341 agcgggtcgt tgacgaagct ctgctcgagg atgcgcacgc cgaagggttt cttgcccagc
    32401 tcgcgcaaca tgacggaatc cggctccaac tggcgcgaca tatccgtcac gcgcacggtg
    32461 gagacgccct ccgtcagttg caggttgcgg gtttccttca ccgtggcgaa cccgtcgtta
    32521 taaatggtga cctgcggacc ggtggcccac gccgccgcgg ggaacaacaa aagggcgatc
    32581 agttttttca tggcgcttcc ttatataatg ggttcgccgt gagcctactc caccccccgg
    32641 acggccacaa tcccaaactg cccccgaatt tttgctggcg aaacgcttca cgttctgcca
    32701 tatcctttat gtctttcggc gcaggttgtg cgcccggaag cccacaagga ttttagacga
    32761 tggctgacct aatcaaaagg ctgggcgtgg cgcttttggc gggcgcgctg gcggcgggcg
    32821 gcagcgcctg cggcaaaaaa aatgcgaccg gcggtgatgt cgccacgctc atcagcgagg
    32881 cgtggaccaa gtatcgcctc agcgagttcc aggatgcgca gctcacgttc gaggccgcgc
    32941 gggccagcgc ccagccgggt tccgacgcgt gggccatggc cacctatggc ctcgccacca
    33001 cgtgggacct gcgccgcccc ggcgaagacc ccaagctcgc caccaagctc tatcaagaaa
    33061 tcctcaccaa cgcccccgcc agccccatga tcccgtggac cgagctggcg ctggcccgcc
    33121 aaaagcatct ggtgcccgtc gggcaggagc ccgactacgc cgccgtgcac aaggcctatc
    33181 aacacatcat ggatgccttc cccggccacc tcgccgccaa ggaggcattc ctttatcaag
    33241 agagcatcga gctggccaag ctggaggaag ccagcacccg cgccgccgtc aagcgcctga
    33301 atcagtttgt gcaatctgac tccaccgaat tcaccggccc cgcgtggtcg ctactggctg
    33361 tggcccacac cacgctcaag aatcccgccg gccgcctgca cgccgaacaa aaatcctttg
    33421 agctgaccga agtggacccc gccgacccgt tcgtggagtt cgcctgggcc tactggaatc
    33481 tcgccaccat cgccgagttt gaagtgggcg attttgactt ggcccgccac tactacaaca
    33541 agctgctgga ggcctacccc aacgacatcc gcgtctatgg ctgcaagcag gccctggcgc
    33601 ggatggatgc gctggaagcg caaatccgcg ccgaagagga gggccgttaa gatgcgcctg
    33661 atccagaagc tgctggactt tgtgcgaacc tacttcgccc tgctcgtggt gttcgcggta
    33721 ttcctgtggt cggcgtgggt gatcgccacc taccgcgccc agcaagcgcc accgggctcc
    33781 atcgtcattc gccttggcca ctggcaactg gaagccagcg tgcgcgaggg cctcgacgaa
    33841 atggcccgcg actaccagaa gctgcacccc aacgtggtca tcattcagga cgccatcccc
    33901 gaaggcacct acggccagtg ggtcagcacc cagctcatgg gcggcaccgc gccggacatc
    33961 atgcaggtcg gcgccatgct gcccgacaac atctggcttt cctattacaa ccgctatttt
    34021 cagccgctga gccggtatgt caataccccc aatccgcaca atgccggcac cgacatcgcc
    34081 gatgtacccc tgcggcgcac attcaaagac ggcatgaaaa ccgcctacgt tgcggaaatg
    34141 caggaataca tgtccgtgcc gctctcccag ttcggcgtgc gcattttcta taacaaggac
    34201 ctgctcaaga aattgaccgg cctcgacgtg gccccgctgg actaccgcga attcctcgct
    34261 gtctgccaga aaatccaaag cgtgaccaac cccaccagcg gccagccata tattcccatt
    34321 gcgggttcgg cctatcactt cggccactgg gatggcatga tgttctcgcc cgtcacctat
    34381 ggctgcttcc gccgcgccga cttcaaccgc gacggctttg tcggcaacga tgaaaccttc
    34441 gtggctttca agactggcca gctcgatttc ctttatccgc ccctcgcctg ctggctcaaa
    34501 atgctgcgcg aggtcaccgg ctacttccaa accggctaca ccggcctcgg ccgcgatgaa
    34561 gccgtgttcc tctttgccca gcagcgcgcc gtgttcatga ccaccggcac gtgggatgct
    34621 cgctccctgc aagagcaggc caaggggcaa ttcgaggtcg gcatcatgga cttccccatc
    34681 cccgcgcgcg acgacccggt ctatggcggc gtggtcgaag gccgcatcta tgaaaccccc
    34741 ggcgccggct tccgcttcgg catcagccgc acctccaaaa actttgatgt ggcgctggac
    34801 ttcctgctct tcctcgcctc ccagcgcggc aacgaaaaac tcaaccgcat catcggctgg
    34861 attccagcca ttgtcggcac cgagctcgac ccgctgctgc aagcctttga gccgcacctc
    34921 gaaggcatct acggcaatat caacttcaac ctcggcggca acaccgccgt cacctggggc
    34981 caaatctact cgctctatca ggtcaaccaa atttcgttcg aggacttcgc cacgcgcttc
    35041 accgaatatt atctgcaaaa cgggctgaag gacttcctgg agcagcagcg cgactggcgg
    35101 cgcggcatgc agcgcaacga acaatatctg gccggcatcc gtggccgcgc catcctggcc
    35161 gacgaagccg ccgccgccgc caccgatccc gccgccaagg ccgccgcgga gctggatgcc
    35221 gcctcggcgt gggtccgcta ccgcgccatc acctcctcgc gtcaaatctg gggcgaactc
    35281 aaccacgccc gccagttgga cctgatcacg cgcgaccacc tgcccgccga ctacgtggcg
    35341 ccttacgaat attcccccgc tgtcctcacc aaaatccgcg aacgcatccg cgcggaggtc
    35401 gcgccatgag acacgccccg accgttttcc acagcgtgga aaaaaacttt ccacagcgtg
    35461 gaaaagtttc cccctgcggc gcattatttt tccacagtgt ggaaaaaaga tttccacagt
    35521 gtggaaaaaa cggcccgatt tttccacgca atggaaaaag ttttgacgat ttttccacac
    35581 aatggaaaat aggcgagtgc cgagcccaag tccgaaggac gccgggcgaa cgcgagctct
    35641 atagcgaccg gcgcagccgg gcgagcaagc gagccatagc gagcgcagcg gcaacgtttg
    35701 aacgattttt ccacgcaatg gaacaacttt gcgggatttt tccacagtgt ggaaaaataa
    35761 tttccacact gtggaaaact gccgcgcgcc gcgccctgaa ccccgccgcc cccacccctt
    35821 cccccgcccg ctgacatgca acccaaagaa gtcagaccca accagctccg tcaccactgg
    35881 gaaatttatt tcttcatcat tcccacgctg ctgctgattg ccctgttcca gtattacccg
    35941 gccagctcgg gcatcttcca ctcgctgttc cgctggaacg ggtcggatat ttccgaattt
    36001 gtcgggtttg aaaattatac cgatttgctc aaaaacctcg agttctggaa ctcgttcaaa
    36061 ctggcgttca ttctcgggtt ttggaatgtc atcaaaatga ttccggcgct gctggtggcg
    36121 gtgagcatcc accgctgcac gtcggagaaa atgcagtttt tctaccggac gctcttcgtg
    36181 gtgccgatgg tgattccggg gctgatcacg gtgctgatct ggcgttcgtt cttcttcgag
    36241 gccacggcgg gctacctcaa ccagttcctc gattggacgg ggctgatgac gctgctgcag
    36301 cacggcggca acttcgtggc ggccaagctg gccgatcccg gcaccacggg cttggccaag
    36361 gccgtgctgg agtcgttcga ctggctgacg gcgttcaagg cgggtagcgc gccggcgtgg
    36421 ctgggcgatc ccaagattat cattatcgcc tgcgtggtgt ggggcttccc gtgggtgggt
    36481 tcattcgcgg tgctgaccca cctggccaag ctgcaaaata tttccaagga catttacgag
    36541 gcggccgata ttgacggcgc caactggtgg acgcgcttta ccaagattga attgccgctg
    36601 ctgatgggct cgatctatct gctgctggtg ttcgccatca ttgatacgat caaggatgcc
    36661 ggcatgatcc tcgcgctggc gggaattacc ggcggccccg gcggcaaagc gaccgtgccc
    36721 gcgctcttca tgctgcgcaa ggcgttcatg gatcagcaaa tgggctacgc ctgcgcggtg
    36781 ggcattgtgc tgacggtggt ggtgctgctg ctgcagaaga tttgcaacat gctgctgcag
    36841 ggcgagccgg aaggcggcgc gcgtccgggc aagtcggcat ggtttgggat tctcgcactc
    36901 tacacatttg cggaagggtt tgtgttcttc cgcgcgcact tggtgccgca cacggccacg
    36961 ccgctcattt tcgccgcgtt tgtggtgacg ctgctgatca agctgggtct gctggtgtcg
    37021 gtgccggtgg cgctgcgcaa gtatggccgc gcgctgcggc acgggttgct ggcggtggcg
    37081 gcgggcctga tggcggcggc catcaccggg cgcgcggatg tcacgtggct gctgtttggc
    37141 tacgcgctgg tgatgatgct ttccaccagc ctcttcaagg cggccatcgg cgtggcgggc
    37201 gcaattttga tcggctacgg cgcgacgggt ggttcgctga acatgatcat ctccggcgcg
    37261 gtattcatca ttctggccac gccgtggggc caattgggcg cgcgggtgga caaggcctat
    37321 ctggcgcgtt gcctgcgccg ccccaccccg gcggtcagca agggcgattt atatgtcgcg
    37381 cgttcgttcg cgcggcagca aatgccggcc tacaaggcgg cggcggcggt cggcagcgcg
    37441 tggctgcgct ttgccaagca tgccttcatc tggttcgtgc tggcgttcgc gttcctgccg
    37501 ctctacctga tgctgattgt atcgctcaag gacaataacc agttctatct gaaccccacc
    37561 acgctgaccc agccgttgca ttgggacaac tggtcaacgg cctgggccat ggtgtcgcca
    37621 acggtggcca actccatttt cctgtccacc acggccacat tcttcattgt gatcttcggg
    37681 ctggcggggg cgtatttctt tgcgcggctc aaggtgccgc tgtcgtcgtt cctctggaat
    37741 gcgctgctgt tgctgatgat gatgccgatg atcgccaacc tcgtgccgtt gttcatcctg
    37801 ctgcgcgaca tgagcctgct caacaccctg accgccctga tcctggtggg cgcgtccggc
    37861 ggacaggcgt tcgctatttt cgtcttccgc aacttcatcg cagatattcc gcaggacctg
    37921 ttcgaggccg cagaaattga cggtgccaac cacttccagc agctcaagct ggtggtgatt
    37981 ccgctctccg gccccatcat gggcaccgtg gccgtaatgc agtttttggg ccagtggaat
    38041 gagtttgtga tgccgctgat tgtcatgcgc gaacaggcga ggctgccagt gactgtgcag
    38101 ctcatccgca tggcgggcga atacatcaag ctgtggggcc cactgatggc cggctacgcc
    38161 atcgcctcca tcccggtgat cattctcttt acgttctcca tgaagctgtt cgtcaagggg
    38221 ctgacggaag gcgcggtcaa ggggtgagca tggagtatag cgcgccattt ttcggtgatg
    38281 agattccgct ggatcatcat ccgctggaaa tccggctgcg cagcctcggg cagttgttcg
    38341 actcgcacga tccctccccc tttctcgcca aggaccttgc tgacgacgcg caggattaca
    38401 tcatcgccag tgccgaagag gtggccgacc gcctgccgct ggcactggtg attcacctgt
    38461 ccgagccgcc tacctcgccg gcggaggcgc gcgatgccgg cgaggccatt cgcgagcatt
    38521 ttgtgcggca gtcggggctc acccgccgcc agttccgctc gctgattcat cacgggttga
    38581 tcagcctcgc catcgggctg gcgtttttga gcctggcgct gattacctcc gccgcgctga
    38641 ccggctgggc cgccgccagc accctgcgcg acgtcttccg cgaaggcctc atcatttgcg
    38701 gctgggtggc catgtggcag ccgctgcaaa tcttcctcta tgactggtgg cccgtcctgg
    38761 gcgagcggcg gctctacgac cgcctcagcc ggatgcccgt gcagattgtg gtgggcgact
    38821 cgccggcgtg aaaatgccgc ggcgggcagg cttgcgcgcg cgggcgcaaa cgggtagtct
    38881 gccctgcgtt ttgttgcgcc gccgcggcgc gacattgtgg aaaggttgcc atgtcccgta
    38941 atgtctatat tgccgccacg gaagccagca gcggaaagtc cgccattgcg ctcggcgtca
    39001 tggaaatgat ggtcgaaaaa gtcggccatg tcggcttttt ccgccccatt attcccgccg
    39061 atcgcaacgg cgccgagcag gacaacgaca ttgccctgat gatcagccac ttccgcctcg
    39121 acctgcccta cgaaaaggcc ttcggctata ccagccgcga ggccagcgag ctgatccgcg
    39181 ccggcaaaac ctccgagctg ctcgacggta ttctggagcg ctataacgag atggcccggc
    39241 gcttcgactt cgtggtgtgt gaaggctccg acttcgtggg ccagacctcc gccttcgagt
    39301 tcaacatcaa cgccgctatc gccaaaaacc tcgccgcccc cgtgctgctg gtggtggatg
    39361 gccagaaggc cgtctccgat ggccgcatga ccacgctgga tttcgccatc cagagttttg
    39421 aagaaaacga ttgcaccgtg ctggcggcca tcctgaaccg cttcccgccg gagcgcctca
    39481 cggagttgcc cgatttgctg gcgaatacgc gccacgccag ccgccccatt tttgccgtcc
    39541 ctgacgaccc cggcctcgcc gcccccatga tgagcgaagt ggcccacgcg ctcggcgccg
    39601 aaacttttta tcgccccgac ttgctggact cgctgcaagc aacctcgttc gcggtggcgg
    39661 actcctcgcc ggagttgttt ttgtcgcaac tgcgcgatgg cgcgctgctg tttatttccg
    39721 gcgaccgctt cggcaacctg ctcggcgtga tgctcaagct gaactccgcg cggccggtca
    39781 acattgccgg cgtggtgctg accaacggct tccgcccgtc cggcgcagtg gctacgctgc
    39841 tcaaagaggt ccgcaacatg gtgccggtgc tgtccatccc cggcagtacc tacgaggcca
    39901 tttccatcgt gtcgcggctg cactcgcgca tttatcccgg cgccacccgc aaaattactg
    39961 cggcgctggc gctgtttgaa tccaacgtgg acacccaaaa gctggtggat ttgctggaga
    40021 ccgcgcagac cgacgtggtc acgcccaagc gctttgagtt cgggctgatc cagcgcgcgc
    40081 aggagccgcg ccagcgcatc gtcctgcccg agggcgagga agaccgcatt ctctacgccg
    40141 ccgaacaggt gctcctgcgc ggcgtggccg atgtggtgct gctcggcagc gagcagaaaa
    40201 tccgcggcaa gatttcgcac ttggggctca acctgccggg cgtggaaatc atccagccga
    40261 cgcaatcgcc gcacttccgc gattttgtca gcagctatta cgagctgcgc aaagaaaagg
    40321 gcgtcacgcc cgacatggcc gaaaccatca tggccgacgc ctcctatttc ggcaccatga
    40381 tggtccacaa gggactggcc gatggcatgg tgtccggctc ggtcaacacc accgcgcaca
    40441 ccgtgcgccc cgcgttgcag ttcatccgca ccaagccggg cttcaccatt gtgtcctccg
    40501 tgttcttcat gtgcctgaag gaccgcgtgc tggtgtatgg cgattgcgcc atcaacacca
    40561 atcccactgc accgcaattg gcggaaattg ccgtgacgtc cgcgcgcacg gccgccacgt
    40621 tcggcgtgga cccgcgcgtg gccatgctgt cctattccac cggcgattcc ggcggcggcg
    40681 cggatgtcga gcgcgtgcgc gaggccaccg agctcgcccg tgccatggcc ccggacctgc
    40741 ccatcgaggg ccccatccag tatgacgcgg ccattgacct cagcgtggcc cgcacgaaga
    40801 tgcccgactc caaggtggcc ggccgcgcca cggtctttat tttccccgat ctcaacaccg
    40861 gcaacaatac ctacaaggcc gtgcagcgct ccgcgggcgc ggtggccatt ggccccgtga
    40921 ttcagggcct gcgcaagccg gtcaacgatt tgtcgcgcgg ctgcacggtg gtggatatcg
    40981 tcaacaccgt ggccatcact gccattcaag cgcaggcgga aaaatgattc cgctgtcgtt
    41041 gagcactcgt tggaatgctg ggcggcacca gtccggccag gccttgatcg aggagattct
    41101 ggagctgggc ctcggcattg tggaactggg ctatgacctg cgcgccgagc tgatccccgg
    41161 cgtaaaggcc atggtggcct ccggcgcggt gcgggtggac agcgtgcaca acttctgccc
    41221 ggtgcccatc ggtgcgccac gcccgcatcc cgagctctac acgccggcct cccccgaccg
    41281 tcgcgagcgc gagtttgccg tcacccacat cagccgcacg ttgcgcttcg ccgccgaagt
    41341 cggcgcgcgc gtggtggtgt gccactccgg caatgtggat attcccaagc tgtcgttcga
    41401 gctgacccgc ctcgccgcgc gcggcgagca gttcagcgcg ccctacgaaa acctgaagct
    41461 caaggcccag ctcgcgcgcg acaaaaaggc gccacgccaa atcggttatc tgaccgaaag
    41521 tttgcagcag ttgctcccgg tgattcagga aaccggcgtc cggctggcgc tggaaaacct
    41581 gccgacgtgg gaggccatcc ccaccgagct cgaagcggaa aagctgatga agcagtttta
    41641 cgcggccggc gtcagcttgt ggtgggatat cggccatgcg caaatccgcg aaaatctggg
    41701 cttcatcaat gcctcgcggt ggctgcaacg gctggagccc tacattgcgg ggatgcacat
    41761 ccacgatgtc ctaccgcccg gccaggacca cgtgatgccc ccccgcggca aagtggattt
    41821 tccggccttg gcgaagatcg ccggaatgga tatggtgcgc gttttggaac cggctcccaa
    41881 taccgagacg gcacatattg tgcaggccat tgcctacctg cgcgaaacgt ggaacttgcc
    41941 cgacgtcaac acaaccagcg gagaaacaac atgaagaaag cattgattac cggcatcacg
    42001 ggccaggatg gctcctatct ggcggaactg ctcctggcca agggttatca ggtcaccggc
    42061 atggtccggc gcgccagcac cgagaccttc gaccgtatcg cccacttcaa ggaccagatc
    42121 agcttgcggc aggccgacct gctcgaccag ttctccctcg tgaagctgct cgatgaagtc
    42181 cagcccgacg aaatctacaa cctcgccgcc atgtcgtttg tgcccacctc gtggaaccag
    42241 cccgtgctga ctggcgaatt caccgccctc ggcgtgacca agctgctcga ggccatgcgc
    42301 atggtctgtc ccaaggcgca cttctaccaa gcctcctcct ccgaaatgtt cggcaaggtc
    42361 atcgaaaccc cgcagaccga aacaaccccg ttctacccgc gcagtccgta cggcgtggcc
    42421 aaggtctatg gccacttcat caccgtcaac taccgcgaat cctacaacct cttcgcgctc
    42481 tccggcattc tctttaatca cgagtccccg cgccgcggca aggagttcgt cacgcgcaaa
    42541 atcagcgatg ccgtcgcccg catcaagctc ggcacgctca aggaattgcg gatgggcaat
    42601 ctggacgcca agcgcgactg gggcttcgcc ggtgattacg tccgcgccat gtggctcatg
    42661 ctgcaacagg acaagcccga agatttcgtc gtggccaccg gcgaaaccca ttccgtgcgc
    42721 gagttcctcg aaatcgcctt ctcccacgtg ggtctcaaat atgaggatta cgtcgttatt
    42781 gatccggaat tcatccgccc ggcggaagtg gaattgctgc tcggcaatcc cgccaaggcc
    42841 aaggcgacgc tgggctggca gcccaaggtc agctttgagc aattggtcac catgatggtg
    42901 gatgccgaca tggaccgcca aatgggcaag acggtcaccg cgtaaaccac acggacgaca
    42961 ccggaggcag accggcagga aaacggcgcg gcacggcatg aaaattctcg tcaccggttg
    43021 taatggcttt gtcgggcggc acgcgcaagc cgccctgcgc gccgccggtc acgaggtcat
    43081 cggctgcgac gtggcctgcc cgccggattc cgcctgttta ccgctggatg tccgcgactt
    43141 gcccgctgtc tgccgcttgc tgacgaacgt ctgccccgac gccattttgc atttgggcgg
    43201 cctggccttc gtgccgttgg gctggacgga gccgcaacgc gtttttgcgg tcaacaccat
    43261 cggcacgctg aacattctgg atgctgtgcg gcaaaccgcc cccgccacgc gcgtgctggt
    43321 ggtcacctcc gcggaagtct atggctccca ccccgccccc gcgccgctga ccgaggacgc
    43381 accctacctg cccgacaaca tctacggtgt ggccaaagct gccgccgacc agtcggcgtt
    43441 gctctatgcc gcgcaccaca aactggccgt catggtggct cgtccctcca accacatcgg
    43501 cgccggccag tccaaggatt ttgtttccac cgcgttcgct gcgcaactgg cggccatcgc
    43561 cgccggcgcg ccgccggtca tgcgcgtggg caatctggat cagcagcgcg attttaccga
    43621 cgtgcgcgac gtggcccgcg cctacgcgct tttgctggcg caagggcgcc ccggcctggc
    43681 ctacaacatc gcctccggcc tcatgcgccc cgtgcgcgat attctcaccg ggctttgcga
    43741 aatttccggc ctccacccgc gcgtggaaac ggaccccgcg ctgttccggc ccaaccgcga
    43801 ccgcccggcc tacgacacct cgcgcatccg ccagcacgtc ggctggcagc cgctaatccc
    43861 cctgcgccaa actctcgcgg atatctacgc cgatatccaa gcccaacgcg cctcatcctg
    43921 aaacccagcg ccaaccatga ggcgaccctg acgtgtcctc ccgccatccc atcttgggtc
    43981 agttcgcgcg ctgggccatc ggcctggccc tgttggcggt tttgctgcgc accatcggcc
    44041 tcgacgatgt gcgccaagag ttcgcgcggg tgcaagatgc gcccggctgg ctggccgcgg
    44101 cgttggcact gacgtttttg gcgttgtttg ccggcgtggt gcgttggcat ctgctcctgc
    44161 gggctatggg cctgcccacc ccgttccacc gctccttccg cggttttttt attggccaat
    44221 ttttcaatgc gttcctgttt ggcgcctgcg gtggagacct agcacgcgcg gttcacgcgg
    44281 cgcacgccca tcccgcccgc cgcgccgaag cggtcacctc ggtttttctg gatcgcgtgc
    44341 tgggtctgct cgtcacgctg gtcattggct gcgggctcct cctcctgcgc ttccgttatc
    44401 tggccaacga tccgcatgcc tcccgcgccg gttggctcat gatggctttt ctggccgccg
    44461 ccaccggggg cttgctgctg ctgttttccc acaatatttt tgcccgcctg ccatggctgg
    44521 cccgctggca gcaccgcggc tggatcggcc cgctcgtgcg ccggatgtat gacgcgcttt
    44581 tctttttccg tcagcatggc cgtcacctgt tttggcccac ggtgctctcg gtggtcaacc
    44641 tcctgctgtt ggccgcggca tctggcgccc tcgcccgcgc tatgaatctg gcgttgccgt
    44701 tcatggattt gctgacggtg ttccccatca tcacggtgct ggcggccatc cccatcacgc
    44761 ccggcgcgct gggcgtccgc gagacgttgt tcatccaact gttgcagccg ttcggggtcg
    44821 cggccggtgc cgcggtgtca ctctcgctgc tcggctattt cgccggcagc gcctggagcc
    44881 tcttcggcgg cctgctgttc ctgctggccc cccgcccgac caacccgccc ccataaaccg
    44941 ccgctacttg cccgccacgc ggctggttga gccacgcacc accaaggatg tcggcagcag
    45001 gtcgctgcac tcggtgatct cgcccttgat gagttgtaaa atacggttgc aggactcgat
    45061 gcccacttgg tgcaagtcat ggcggatggt ggaaagcccc ggatttacaa acgcggcgag
    45121 ctgaatgtca tccacgccca tcaccgaaac atcttccggc acttcgcgct tgttatccac
    45181 cagccaacgc atcgcgccga tggccatgcg gtcgttggcc gccatgatgg cggtgaggtt
    45241 gggttccatg gccagcagct tggccgccgc ggcgtacccg ccgcgctggc tccactcttc
    45301 gccaccgtcc acgctggcca cctggcccgt cccggccggc cagctcttgc acacgcggat
    45361 aaattcatca cggaattgca ggcccgtgta cgtattggta ccggcgatca gtccgatctt
    45421 gcgatgccca aggcccagca ggtgctccgc cgccagccgc gccgaagcca cgtaatccac
    45481 gctgacgtag ttcagctttt ttccggggaa cagatggttc accagcaaaa acttatacgg
    45541 gctgttttca aacgcggtca gatattccgc gtgaatgtcg gaggcaataa acaacatgcc
    45601 gtccgcgcgc cggctggcca gcagcttggt gtattcctgc gacttgatga attgcgccgt
    45661 cgcaatatcc aacagaatct tgtagccctg cgacgccgct cgctcgtaaa taccggaaat
    45721 aatttcgccc agataaatgt ccgaaaaaac gcgcgtcaga ttcggcacca ccaccgaaat
    45781 ggtacggctg gattttgagg cgaggccgcg cgccgcgctg ttcgggaaaa agttgtactt
    45841 tttcaccacc gccatcaccc gctcgcgcgt ggcctggctg atgcgcggat ggttgttcaa
    45901 cactagtgaa acggtactga gtgaaaaacc gctttccttg gcaatatcct tgatggtttt
    45961 acgtttcata agacaatccc tcgcttgatt tgacgtaaaa catgccagtc aaccgctcaa
    46021 cagagcaagt aaaatgtttc gcattttacc ttgtgggttg tcccttattt taagagtgca
    46081 ttgggtggat aatcttggga tgctgcgggt ttatagaata ccggcaggcc cagatgggcc
    46141 ggcggaatca tcaggaaacg aggtgcttat gcgcaaccca attttagttc cggaattgct
    46201 ggagtttatc caggcgggca atcccgccaa agtgcaggag gtcgtggatc acgtccaccc
    46261 cgccgacatg gccattttca ttgcggcgtt ggaagatgcc gatgtttggc gactgctggc
    46321 catgatgagc atccagcatg cggcagccat tttctcgcac ttcgatttgg aacggcaggt
    46381 tgaattggcc agcgatgcca accgcgcgga aatggcccgc atgctggaag cgttgccgca
    46441 cgacgatcgg gccgatctga ttaaccaact cgatccacag gtggcggatc agttgctgcc
    46501 gctcgtcgcc cgcgccgagc gtgaagacat tcgcaacctg acgagctacg aagaaggcac
    46561 cgccggcgcc atcatgagta gtgattacgc ggcgctccgc cccgatatca ccgtcagcca
    46621 agcgctcgac caagtccgta ttcaagcccc cagccgggaa acagtgtatt acatctacgt
    46681 ggtggatgcc gaccatcggc tgatcggttt cgtctccctg aaggatttga ttctggccag
    46741 cccggtacag caagtacgcg aagtcatgca caccgagatc attcaagccc gggccggtga
    46801 cgcccaggag cacgtggcgc agctcatcga aaagtatgac ttgctcgcac tgcccatcac
    46861 caatgccgaa gaccggctgg tgggtatcgt gaccatggat gacgtgctgg acgtgcagga
    46921 agcagaagcg accgcggcat tccacaaaat cggcggcacc acgctggcag atgaaaatct
    46981 caaggaagcc ggcttggccc tgttgtaccg cgcgcgcctc ccctggctgc tggttttggt
    47041 gttcatgaat gtgttggccg gtgcgggcat cgcctattat gagaatacga tcatggcgat
    47101 ggcttcgctg gtctttttcc tgccgctgtt gattgactcc ggcggcaacg ccggctcgca
    47161 ggcggccacc ctgatggtgc gcgcgcttgc tgtgggcgat gttcgcatgc gcgactggtt
    47221 cagcctgctg cgcaaagaag tgctggtttc gcttttcctg ggcctgacca tggccgcggg
    47281 ggtcatgctg gtcgcccaac tccattcacc ggaaattgct atccccgtgg ctttgaccat
    47341 ggtcagcgtg gtgctgtttg gcagtttggt gggtatgagc ctgccgttcc tgctgacgcg
    47401 cttcaagctc gaccccgcca ctgccagcgc accgttgatt acttcgctgg tggatatcgg
    47461 cggtgtgctg atttatttcg gcattgccac atggtggctc aaagatgcca tccacgcggc
    47521 cggcagctag aaccacattt ccgggcagat cttcggcgac acaacaaact ggatatctct
    47581 accagtctgt tctccctcca tcctctccct aggggctcac tctatgccga cacggtagaa
    47641 gttttgcggc agtagcatgt tggtaatgga cagatcaatc gcgccgccgg taccaggccg
    47701 cccactaccc gtcaccttct gccaattgga cgacggaaaa agcgtcgggc tctcccacag
    47761 ccaatacgtg gcccctgcct ggctcgtcaa aagcgacagg ttcagcccac ccgtttcccc
    47821 accttgcaac tggatgttgg taatgttagg caccccgcta gctgcccgcg tgatcgtcac
    47881 cactgcgctg gtgctaacta cggtgctacc caagctcaac accttgatgt cgtcaaagaa
    47941 aaagtcgtag ttcgacccct ccccgccgtt ccagttccac cagcgtgccg ccaccaatgg
    48001 gccgttgtat gtcccggaaa ataccgccga accaactcgc aattcatagc tgttggtcgt
    48061 cagcagcgtg atggtcactg tttggggcat atccgtccac gggataccac tgttgcggcc
    48121 gccatcggcg tcttcaatct gatattccac atcgccaccg ttgaagtaga actggatggc
    48181 gtccgctccc tgctggttgc gcagcatcat cccaacccct tgctggcctt ccagaaccca
    48241 gccgtttttg agggtggctt ggaattgctg gccgggcgcc aacggcgcca cgaacggacg
    48301 aatggcttcc gcgagattat tggtgtgcgc ccagagcccc cacccatttt cgctggtgaa
    48361 gaatccggcc tccgagttat tggcgagtga ccaggcacca aatccgctgc cgccgttttg
    48421 tccggtgagc caatcgccca agtaagccgc attgctggct atatcctccg caatgggttc
    48481 cattatggcc tgcgtgttgg tggcggtcac ggtgatgaca ttcgtcccct cgttcagcgg
    48541 cacattggcg gctgtccagt tagccgacag aggaatggca ttgctgctgc cggtcaggtt
    48601 gttgctccag aacatcccct gccagttgtt cgccatcagc gtaccctgca ctgtgtaggt
    48661 cgtgacctcc ccgcccacgc tgatatttcc cgccggattc gtaattacca actgcggcgg
    48721 cggaagctcg ttgtccagaa ttgtgactgc aatacggttg ggcgatccaa gactagcccc
    48781 cgtcactcca ctcaggttta cataaaagac ttcatctccc tcataaacgg cgtcgtccaa
    48841 aatcgtaata tccacatatt tctgtgaatt gtcctgatcc gcccagttca agattccgga
    48901 tttggcggtg taatcgctcc ccgctgtcgc cgtgccgttc gaggttgcgt agttcacgct
    48961 cgccgcacca tccgcgccgc ccacgcggtt gacaaatacc cggactgtgc ccgcgttttc
    49021 tgccacattc gtagccgctg tgctgaattc aagcttcccg ggctgaggct caggctccga
    49081 ctcttccagc agctcgttgg gttcgtccca cggattgtac agataatgaa acgccgcccc
    49141 aacgcgctgc gaccaagccc attcgtcatg cccatgccca cagccaatat caatccggat
    49201 gtcatcgcca accacatagc catcctgtaa ataatagcca tacaccggcc aaaaataatc
    49261 ccacatactg gagccctcgc tagtcccgca atcttgccag atgcgccggc ccttggtctc
    49321 attgtcgcga atccagttgc gaaagttggt ggcataccaa taagagggcg acacagctcc
    49381 aatcaggcca aacacgttgg ttttcagccc gatatatgcc gagatcagtc cgcccagcga
    49441 ggagcccatg ttgccagtgt tcatcatgtc cgtcagggtg cggtagttgg catcaatgtt
    49501 gggtttcacg ttgttgatca ggtagagcag ataataattg ccggtgtggc cgtcttccgg
    49561 cgttccatac tcatccatcc ggttcgtcgt gttgttaacc gccacgatga tcgtctcgcg
    49621 catccggccc tgcgtgattt cgcgcgtggc tgcttgatcc gcagaccacg atccaaacgc
    49681 cccgcccgga tcgaatacat tttgcccgtc gtgcatgtag agcaccggat accgcttgct
    49741 cgaattttgc gtgtagccgc gcggcagata aatgcggcca ccgcgcgagg gaatgccgtt
    49801 gccggcatag ctcgacgccc agttgccaac gttgatgact tgcggcgcgg aaaccgttgc
    49861 cggcggcaca taattaaaaa tattaccatc ctgcacgaaa aacgcgtcca gccaggtgta
    49921 gtagtttggc gggtcaccta ctccgcctac tgccggattg tcccatttct ccactccccc
    49981 gtcatagccg tgcatcacga actccaacgc gcggcccggc tcacccacgc cggtcacttt
    50041 gtaacgtcgc tcgtttccac cacgccctgc ccccacgtcc cccatcacgg ccgagtgctg
    50101 ccatgtttct gtttccccca cgcggtgaaa caccgtcacg ttggtccatg tcgtatgata
    50161 ataaatcgtc ttgcccgaat agcccggatt ccacagcggc acattggtgg ttaaacctgt
    50221 gcccgacaaa ttgccattgc ccgtcgtgca atagctcccg ctgctggtgg tgcgcggcgt
    50281 aaagcgatag gtcacttgtg tcgaaaccgt ggacccctgc accgccacct ttgccgacca
    50341 ttggttgttc gacgacacca ttctaacgga cccggtcgtc gtcatgccgt tcagctccgg
    50401 gcggtctacg gccacatgca gcgcattgcc gctgcctacg ttggaactga cgttaaagtt
    50461 cagaatatgc agtggcggcc cgctccccca cgcccccgtg gcgatcccca tgcccaacag
    50521 gctcaaaacc agcccgtttt tcagtgcgca acaagataac gatttcattc caccacggta
    50581 aaatacgccc gcgccgaaag caaacccgga cgatccgttt tcgagcgcat tttacgcgcg
    50641 gcgcggcggc gcacgattgc gttacagcag tggcgcaagg aggccgcagg cattttccag
    50701 ataacggtgg cgaatcggac gctgtttgaa cgccgcaagg tccaccggct cgctggccgc
    50761 caaatcttcg atcatgatca acttgaggtt catcaccaac tccgtgtcat acaccacgag
    50821 gctggtttca taattggaat acaaactgcg atagtcccag ttggacgaac ccaccacggc
    50881 catccggtca tccaccacca tcgctttggc atggatgaat ggcggacgac gctcgttgat
    50941 ctgcactccc gcctccaaca ggtcttcgta catcgcgcgc gcggcccacg tggtatacca
    51001 gtggttgctg gttttcggca ccaccaacac cacacgcacc cccctcaatg ccgccatgcg
    51061 caacgcgcgc aataaatctt ccgaaggcag aaaatatggt gtgatcacaa acacctgttg
    51121 ccgggccaaa ttcaccgcgt tgaaaaacac gtccacggcc aaccgcaaat ccgacgatgg
    51181 cccgctgttg accacgcgcg cgaatgcctg ccccgccggt ggctgccgct cgaagtaggc
    51241 cgcactcaac agttggtctg gattttcttc ggtcatgacg tgccagtcgc gcaggaatga
    51301 atactgcaac tcctgcacca ccggcccctg tacctggaaa tggtaatcct gaatggccgg
    51361 cgcgccgggt tggctgcgcg agtagccatt caaattgaca ccgcccatga accccacgcg
    51421 cccatccacc accagcgttt tgcgatgatt gcgcagattg aaatgcaact gtttgcggaa
    51481 caggttggat tgtgtccacc cggcaatcgc catgttgggt acccgccggt aacgccggaa
    51541 caacccaccc cacagcgcgt gggatgagcc gaaccggtcg tagagcacac gcacgcgcac
    51601 cccctcgcgc gccttgacgg ccagcgcctc catgaactcc cgccccacgg gatcgttgcc
    51661 aatgatgaac gattgcaaat gaatatgatg ctgtgcgccc cggatggcag ccaacatggc
    51721 ggggaacgct tcgtcgcccg tcaacagcgg tgttacatgg ttgccgccca gcagtggaaa
    51781 gttctccagc atggcggcca gtgggcggtc cagttcctga atccattcgt ccgccggcgt
    51841 ggccgctgcc cgtcccatgg agcgccagta ggcctcgggt acaatctctg cgccggccat
    51901 cgtttgcgcg tcatccatgc gctcgctgcg ggccacgctg cggcgccacc gttccttggg
    51961 tacgcggtcc acgccaaaca tggcgtagag cagcgcgccg attaccggca tggaccacgt
    52021 gacaaacagc cagagcaacg tggagtccgc gcgccgccga tgccgcaaca catgcagcgc
    52081 caccgccaga aagagcagca ggtgaatccc cgcgccggct gaaagccacg gcgcgccgcg
    52141 aaaataatca gtccacgcaa caccgctcat aacggtcagt gtggcgcagc gccccccgcg
    52201 ccgtcaagcg tcggcgcctg gcaaaatcaa ctttctccgc gcaggtgcgc cgggagatca
    52261 gggcaacatg cggcggaagg cgcgggcgcg ggcttccaac tccgcccggg tggcatgccg
    52321 caggcggtcc acgctgaacg cctcgcaggt gtaggccgcc gccaccgtgg cattgaccag
    52381 cgccgtgtgc aggttttcgc gcgtcacatc gcccaatgcg gcgagagtgc cgatgagccc
    52441 accggcaaag gtgtcgccag ccccggtcgg gtccaccact tccaccaagg gccacgccgg
    52501 tacaatcaga atgtcgtcat gcgacatcaa aaacgcaccg tgctcccctt ttttgatcag
    52561 cgcatatttg gggcccattt cgaggatttt gcgcgcggcc ttcagcagcg aacccttgcc
    52621 cgtcaggtgc cgggcctcgt gttcgttgac cgtcaacagg tccacgcgtc gaatcacatt
    52681 catcaattct tcgcgcgtgg tgttaatcca caggtccatg gtgtccagca cggtgaactt
    52741 gggcttggcc atctggtcaa gcacccgggc ctgcaaggcc ggagcgatgt tttccaggca
    52801 caaaaacggg ctggtgcgat atgcctccgg aatcaccggg ttgaaatcgg caaacacatt
    52861 caaatcggtg ctgttggtgc gccggttgtt catgttgtct tcataaaccc cgctccagtg
    52921 gaaggtcttg cctgccgcct gttgcagtcc ttgcagatca ataccggccg tccggaaaat
    52981 ttcaaaatac tccggtggga aatccatgcc caccacgcct actacgccag ttttggcaaa
    53041 aagcgctgag gcaatgctgg tgtagctggc tgagccgccc agcagctttt cgtgcgcggc
    53101 cttggatgtc tgcacgctgt caaacgccac cgaccccacc acgaccacat ccggaaccat
    53161 ggaattcgct gttgttgtca tgaggaacct ccgttgccgc gcggcgcgcg gtgtttagtc
    53221 cgccaacttg tcttcaatca actggcacag cgcatggatg atcatgatgt gcccttcctg
    53281 aatgcgcgcg gtcacccccg tgcaacccac gcgcaacacg gcatcggcca tggcggccaa
    53341 cgccccccct ttgccgccgc acaaggccac ggtccacacg ccggctgcct tcgcggcttc
    53401 gacggcgcgg cacacgttgg ccgcgttgcc gctggtggag atggccagca gcacgtcgcc
    53461 cggccgcccc aacgcttgca cctgcttggc gaaaatctgg tcaaagttgt agtcgttgcc
    53521 gatggaggtc aacgtggagg tgtcggtggt cagcgccaaa ccggcgtagg gttggcgttc
    53581 gcgttggaag cggttgacca gctccgccgc aaaatgctgg gcatccgccg cgctgccgcc
    53641 attgccacag gccatgattt tgttgccctg ccgcagtcgc tcggccatgc ccaccgccac
    53701 tgcttcaatc ggcgtccgca gcgtggtcag cacttcgtgg gcaacatcca ggtgcccctc
    53761 ggcaaaaaca tcccaatcca tgaagcctct ccctacggca ccgcactgtc cggcgtggat
    53821 ttcttccgac gctggatttc gttgacctca atggatacgc ctgccagatc gcaggtgcca
    53881 agatgctcag ccaacagctc gcgcacccgg gcctgcatca gtttgcacag ctcgggaatc
    53941 cgcgtgccat cgcgcacacc gaggatcagg cctacctcca gagcgccgcg atgcacccgc
    54001 agcagcgact tcagccacac cacggcggca aactcggttt tcaacccgcc caaataatcc
    54061 cgcaccgcat cagcgctgac gttgatctgg ccgttttcat tctcgaacgt gatgaacgtc
    54121 cgccgtcgcc gccacgccac accgctcaac agatagatga acagatagcc cagcaccagt
    54181 accccggcca ccacgcggac ccatggcggc aaactggcgc caaagcctac tgccagtttc
    54241 aggcattcgg gataggccgg cgacacaatc agcaccagcc cggccgctgc aatgcccagg
    54301 aataacaaca agcccaacaa aaaatgaatg gcgcgcatgt gatctctcct gtttccattc
    54361 aatctatacc ccgccaacgc ccatttgccc aataaaaaac gccccgccca gcaaatcggc
    54421 cctatggcgc ggaaatagtg accggttctg gcagttgctc cagcaggcgc cgggcctgtg
    54481 ctgctggcag cacccggtag gccggcgccc gggcctcggt ataacccacc gaatggtgcg
    54541 ccgccgggta gcctttggat ttcatggctc gatccacccg ctgccactcc gcaaaggtga
    54601 gctgccccac cggctgcgcc tgcaagacac gacctaaagc gcgccatgct tgccgaaaac
    54661 tccctgcatg gccgtcaaaa tcccgcgcgc ccgccaaaaa agcttccagc agcgcctctt
    54721 tgttcccgcc ttgcgcttta tagggccgca ggtgcaggcg cccgattcga ccgtccgcgg
    54781 tcaacggctc ccacaactcc tcgccgggtt gcggaggacc aagcgtggcc cattcacggg
    54841 ccagccagag tccggcggca gtttcatcgg ccaaggcgtg ctcgcccccg cgcgcggcgt
    54901 ggtataaata tttgtaggca tcttccgcgg caaccgctgg atcctgcgcc ccgtgagcca
    54961 gaaaataggc ggtcaattca tcttgggccg gggcacaagc caaccagccc aaactcaccg
    55021 ccaacagcca acgccgcctt ttcacgtggt tcaccctacc ggccactcgg agcagcgtca
    55081 atttgttctt gagaaaacac cttccagcgg tagtattacg atcatgaaga aaagtaataa
    55141 taacaaatcc gactgtgccg cccgcttgag cctctccctg cccacaccgc tggcggccga
    55201 cttggatcgg ctggtccgcg ccaaaggggc ccccagtcgc tccgccctat tggccgacct
    55261 ggtgcgcgag gctgtcaccg agcacgatgc gcatcgcggc cggcaaaccg ccgccggggt
    55321 catcacgctg atctatgacc accacgcccg caatttgcag gccaaactga ccggtatcca
    55381 gcacgatgcc gaaaaaatga tcatttccgt catgcatgtg cacctgtccc accacgactg
    55441 cctcgaaacc atcgtggtcc gcgggccggc tgctcatctg cggcaactca ccgaccggct
    55501 ggccgccgtc cgcggcgtca agcacggcaa gctcaccatc accacaaccc aaaaatagga
    55561 aaccatgaaa gtgaaaacgc ccatcattac ccccgtactg tggcttgccc tgatcctcct
    55621 gctcgtaatg ggcatgaccg ctttagccca actgcccgtg gtcacccatg ccgaattgca
    55681 agccgtcaac tccactggcc acacggcatg ggtggaatca ttcccgtttg tcatccgcgg
    55741 cgttattatc aacgaccccg aggaaatgct ggactactcc tacgatcccg atgcgacggt
    55801 ctccaaccgc ctcggtgggc aatatcagat gttcttccaa ggaattggcg gcgaccgcgg
    55861 cggcaccgcg ctctggatgg cgcaaaacta tcaggccatg ggccaggcgg gacaggacta
    55921 cggcacagag tggaccaacg aaatgcgccg cattcagtac gatgccaacg ggcgcaagtt
    55981 ccgcaagggc gacttgattg aggtcactgc tcgcaaggcg ctgttccgtg gcggcaagcg
    56041 caacatcaat gaagcgcacc gcatcaccga gtccaacgat tttgatgtgg tactggtgaa
    56101 ggcgaatgtg ggcgtcccct ccgccgagcc catcaccttg gccgatttgg tgaatcccga
    56161 cggcacacaa atttttgatg ccacacgcgc gacgggcggc gagcattggc aaggtatgcg
    56221 ggtgcgcttg gaccaaatcc ggctctcgac caccaacggc tggggcaaaa ccaactgggc
    56281 cgaccggatt tgcttggcgg ctgatcaatc aggccgcacc ttcccgctac gcatgccgct
    56341 gctggatttg ggcccgccca aggccaccga tgtatggttc agcgtcaccg gcatcatcaa
    56401 ccaagaaaac agtaatacca acggttacga gctgtttgtt caggaagtgg gcccggagtt
    56461 gcgcatcacc caaggcgcta atggccggcc ggcggtatca ttctcttcag attacgatgg
    56521 ctacgtcctg caatactccg acgatggcct caacacctgg gccgatttgg atgccacgcc
    56581 ggtcaagacc atcatcattg aagatcaagg cgattcgatc aatcggatgt atcgtttgat
    56641 caaaaaagag gaatagtgcg ccgaggattc tccatcattg aattgaccgt ggtgctagcc
    56701 gcgctggccc tattgctggc gctggccgcg cccatgctgg cgcgggccat tgagcaggcc
    56761 cgtgtggcgc gggtggtcaa ggacttgcag acggtggagg tggcgctgga agcctttcgc
    56821 acggatcacg gccgctaccc gccagtcgtg atgtcgtgcg acaattcaga ccagcaggag
    56881 gttctccaat tgccgttgga actcgccgac ggcaattacc tgccgcgcag cccgcgctcg
    56941 gcatcatcgt cgctgctggc cgatcctttc cggccgcgca gcacatacaa gtatgtcgca
    57001 ccggaaaatt attggatgaa tggctccttt caggatgacc gctaccccgt ctgggtgccc
    57061 aacgacttcc cctcttgcca tgcggcgggc ggcaaggcgg acgattcgcc caactcgccg
    57121 gtggcctggg cagtatggag ccccggcccg cggcccaacc aactcaaagt ccccagctac
    57181 aagttgccgc tggcggccga ggggtggtac accaaacctg gcgattacgg catcattgcc
    57241 cgctacaaag tgcgcaacgg cgccacgtgg agttctctgc gctgactgtc gcttcccccg
    57301 tcaccaatcg cgtgatgcag aggtttgggc tcgcgcgcga cgccgtcagc ggcgggcttt
    57361 ggcgcagtaa tgcgtgtgca gcaaggcgtg cgacttcggt cccaacggct ggcccaagaa
    57421 tgtgcggtac agctcctgaa tggccgggtt gtcatgtgat ttgcgcagcg ttttggactc
    57481 gtcttcctca taaatggccg cgatgcgccg caggcgcacg ctgtcatccg tgaagcgcgg
    57541 ctgcccgccg ccgccgatgc acccgccggg gcacgtcatg atttccacaa agtgatattg
    57601 gacctcgcct gcgttgatcc gctccagcaa gcgccgggtg ttgcctaatc cgtgcgccac
    57661 ggccacgcgc agttccaccc cctccaggaa agcccattgc tgcgtagtgt ccgtgatggt
    57721 gagcaccgcc tccttgatgc cttcgagacc ctcgatcggc ttgatgtgga gattgtcggc
    57781 cggcaattcg cgaccggtca cgatttcata ggccgtgcgc aacgccgctt ccatcacgcc
    57841 gccggtattg gcgaaaatat ccgccgcgcc ggaagacaat cccagcggcg catccatctt
    57901 gctatcgggc aattcggtaa aggcgagatt cgcctgccga atcatgcgcg ccagttcgcg
    57961 cgtggtcagg acgtaatcca cgtcctgcac accgctcgcg gtcatctcgg ggcgttgggc
    58021 ctcatacttt ttggccgtgc agggcataat ggacaccacc accagatcct cgggccgctt
    58081 gccgattttg gccgcataaa acgtcttggc cactgcgccg aacatttgct gcggcgattt
    58141 gcacgtggac aaatgcgcca gcaagttggg gtaataatat tctgcaaagt tgatccaccc
    58201 cggcgagcaa ctggtgaaca tcggcagcgc ggcggtcggg tctttatcca tcagcgcgcg
    58261 tttcaggcgg ctcagcagct cggtgccctc ctccatgatg gtgaggtccg cggcaaagtt
    58321 ggtatcgaac accgcggcaa agcccatctg acgcagggcg gtcaccatct tgccagtcac
    58381 ccgcgtgccg ggcgggtagc caaactcttc gcccagcgcg gcgcgaatgg ctggcgcggt
    58441 ttgcacaatc actgttttgg ccggatcgcc caacgcctgc catacttcgt caatgtggct
    58501 acgctcgctg atggcgccca ccggacaaac cgcggcgcat tggccgcact gcacacaggt
    58561 cacgccgtcc agatcgccgg caaatgccgg gccgatgacc gtatcgaacc cacgcccctg
    58621 cgggaacagt gcgcccaccc cttgcacctc gctgcacacg gtgacgcagc ggcggcattt
    58681 gatgcacttg gcgttgtcgc gcaccagccc gggggtgctg gcatcgtgct tgggccgagc
    58741 cttggcgccc tcgtaggtca cggtgcggat ccccatgtcg ctggccagcc ggcgcagttc
    58801 gcaatcatcg ttgcgctcgc aaacctggca attgccgtca tgctcggaga gcagcaactc
    58861 caccacctgc ttgcgcgcgt tgcgggcgcg cgcgctattg acgtgaacaa ccatcccttc
    58921 cgtgcagggc gcggaacaag ccgtcaccaa cgtgcgggcg ccctccacct ccacgaggca
    58981 cacccggcac gcgcccaacg ggtcgcgttg ttccaaatag caaagcgtcg ggatatgaat
    59041 ccccgcctgc cgcgccgcct gcaggattgt ggaaccttcg gccaccgcca aatcccggcc
    59101 attgatgcga atatgaatca tgttgccagc cctcgcacct gcccggtttt accgtagtcg
    59161 caccgcaggc accggtgcgc ctgccgctgc gccgtgacct cgctccacgg ctgctccact
    59221 tcgtcgaagt tgttccgccg ccggtccagc gcaatcaagt tgggattctc gcgcgggtat
    59281 ggcgcggggt cggcttccgg atcgtagtcc gtcggcacat cgggataggt ccgccagaag
    59341 gcgtgggttt cacccgtcag cagggcatcc atgcctaccg ccgcgcgttc gcccgccccg
    59401 atggctgcca ccacggacga cggaccgctg actgcgtcac cgcccgcgaa cagccagggg
    59461 atggccgttt gaccggtcac cggatccgcc tgcaaccagc cgccggccag tggcacttcc
    59521 agatcgccca gcacgttgcg cgcatccagc cgttggccga ccgccaaaat cgcctgatcg
    59581 caagggatgg tttccagcgc ctgctcgtca tcagcctcgg cacgtcgccg cccgctgcgg
    59641 tcgaattcgc ccagcgtcat ctggcgacag accagcccgg tcacccggcc atcgcgcacc
    59701 acgaactctt ccggggcgac gagcgtgcgc agctcgatac cttcctgttg tgcctcttcg
    59761 atttcctcga catacgccgg catttgttcg cgcgtgcgtc ggtacacgac ggtcaccttt
    59821 tgcgcgccaa ggcgcagcgc agtccgcgcc gcgtcaatcg ccgcgttacc cccgccaatc
    59881 accaccacat ttttgcccac cggcaccgag ccgcggatgt tgtactgccg caggaagtcg
    59941 agcgaatcca ccacgccctt ggcctcgtgg ccggggatgg ccagattcag cccttccggc
    60001 gcgccaaccg ccaagaaaac ggcctcgtag ccttcctggc gcaaatcggg cagggtgaaa
    60061 tcgcgcccca accggcgcag ggtttgaatt tccacgccca gcgattcaat catgcggatt
    60121 tcgcgcgcga gagtttcgcg cggcaaccgg taggctggga tggcctgcac cagcatgccg
    60181 ccggggcgcg cttcggcctc aaaaatcact ggtttgtaac ccaggcgcgc gagaaaatat
    60241 gcacaggtca gcccggccgg tcccgcgccc acaatggcca ccttgcgcgc ggcattggct
    60301 tcgttctcgc ggacttccgg tttcacaatg gtcacttcct gatccaccat gaagcgcttg
    60361 atgctgcgaa tggccacggc ttcatccagc gtgctgcggc ggcaatggtc ttcgcaggtg
    60421 tggaaacaca cccgcgcgca gaccgcggca aacggattgc gttcgcggtg caactggagg
    60481 gcctcggcgt agcggccttc gcccaccagc gccacaaagc cgggcacatg cacgccggcc
    60541 gggcaggcgc tcaggcacgg cgccagcacc agtcccgggc acacccccgc cacgcaatgc
    60601 ttgtcgcgga tatgctgcac atattcctcg cggaagtgcc gcaacgtgga gagcaccgga
    60661 tttggcgcgg tctgccccag cccgcacagc gaggcctcct gaatgaaccg ccccagttgt
    60721 tcgaggcgat ccagatcggc cagttcgccc ttgccctcgc aaatgcgctg caagatttcg
    60781 cccatgcgct tggtgcccac ccggcatggc gcgcacttgc cgcaggattc ctcctgcaca
    60841 aattcaataa agaaccgcgc cacatccacc atgcaggcgt cttcatccat gacgatcagg
    60901 ccacccgagc ccatgatggc gcccaattcc tgcaagcttt cgtaatccag cggcacgttg
    60961 agattttcct tggggatgca gccgccggaa ggcccgccga tttgcgcggc tttgtaggcc
    61021 ttgccgccgc ggaggccgcc gccgatgtca tagatcaatt cgccgagcga cgtgcccacc
    61081 ggcacctcca ccagccccgt attctgcacg cagccggcca atgcgaacac cttggtgccc
    61141 ttgctcttgg cggttcccca ctgcgcgtac caagccgcgc cgttcagcaa aatggccggc
    61201 acgttggcat acgtttccac gttattcagc aacgtgggct tgccccacag acccttttgc
    61261 gcggggaatg gcgggcgcgg gcgcggttcg ccgcgcagtc cctcaatgga ggcaatcaac
    61321 gccgtttctt cgccgcagac aaatgcgccg gaacccatgc ggatgtccaa atcaaacgag
    61381 aggccgctgc cgaggatgtt attccccagc agcccgcagg cgtgggcctg gtcaatggcc
    61441 ttgcgcaggc gcgccaccgc cagcggatat tctgcgcgta catacacata gccctgcgcc
    61501 gcgccgatgg tgaacgcggc aatggccatg ccctcaatga cgctgtgcgg gtcgccttcc
    61561 aacgtactgc gatccatgta ggcgcccggg tcgccttcat ccgcgttgca cagcacatat
    61621 ttcaccgtgc ccggcgcccg ccgcgtgaag tcccacttca taaacgtcgg gaagcctgcc
    61681 cccccccgcc cccgcagccc ggaacttttc atttcctcaa tgacctgctc gggcgtgagc
    61741 gaggtcagca ctttggccag cgcggcatag ccgtcccggg ccacatattc ctcgatgcgt
    61801 tccggatcaa tccagccgca attccgcagc accacctggg tttgccgcgt aaagaacgga
    61861 atatccgtgg tcttgggctg caaggcgttg gtttgcgagt cgctggcgca cagccgctcc
    61921 accacgcgcc ccgcctgcac gtgctcgcgc acgatatcgg cggcatcttc gggtttgact
    61981 ccctgataga aagtcccgtc gtccatccgc aacacgggtc cccgcacaca gggccccatg
    62041 cagccggctg tgcggatggc cacatccttg gccacgccgg cctcggcaac ggcggattgc
    62101 agggcatcca ccaccttttg cgcgccggcc gccagacaac tgccgcccac gcatacccgc
    62161 agctcgtgct tgggcactgg ggcggtccgg agttggtcac gccaggcggc cagcgctgcg
    62221 gctgtgccga tttttttcaa gcgcgcggtc atgacggctc cgtttccgcg ccctgggcgc
    62281 gggcgcgata gctttccagc agctcgccca gcttggccgg cttgacccgc ggatgcaggt
    62341 catcgttgat catcagcacg ggcgccattc cacaagcccc gaaacaccgc cccacttcca
    62401 acgagaacaa acggtccggc gtcgtttcgc ccacgccaat tcccagcttg gcttgcagcg
    62461 cacgcaggac ttcgctgccc ccacgcacat agcacgccgt gccgaggcac acacgcacca
    62521 agtagcgacc gcgtggcacc gtcgaaaaaa atgaataaaa tgaaacgacc cccgtgactt
    62581 cagaatacga tttatcgaat gcagccgcca cgcgacgcag cgccacttcg ggcagataac
    62641 caaagatagc ctgcgccatc tgcaaggcgg ggatcaggcc gccgggcttg tcccgctgca
    62701 tggccaaggc ttcgtccagc cgggccagca actccgcctc atcgtgagcg gcggctgcag
    62761 cgcagccggc gcaggtttcc ggttttgcca tggtggtctc ccgctggccg gcgacggccg
    62821 gcaccgccgc cgtgatcttg ttggtttaaa acgtgccggt aatatacttt tccaagctgg
    62881 caatcgtcgc ctgctgcgaa tcgagcattt ccttgacgat gtccccgatg gaaatcagcc
    62941 ccgccagctt gccatccgcc agcaccggca gatggcgatg ccgcccggag gtcatcagcc
    63001 ccatgcactc ttctgctgtc tgttgcggag tcaccgtgtg cattttggcc acactggtca
    63061 tgacctcctt cacgaggcgg gtcttggggg atttgcccgc cgcgatcacc ttccacaggc
    63121 agtcgcgttc ggaaataatg ccggagagct tgccggtttt ggtcaccacc gcgaggcagc
    63181 cgatatgctt ttcactcatg cgccggatgg ccaccaaaat ggattcctgc gccgtgatcg
    63241 tttccacttg cgagcccttg cgccgcaaca gatcgccaac cgtagtactc atgccttgct
    63301 ccttggaggt ccactgtttt tgaacagaat cactttaggc aaaaacaacg cctgagtcaa
    63361 cgcccaccac cgccaccgat tattatttcg gctctttgcg gcggacaagc taacgtgttc
    63421 cgtgtcgggc ctccgatttt tcggtccacg gtttcttgac gggatggatt acaccccgcc
    63481 cgggctgctg atccccggat tttttcggcc acccccactt ttccacagtg tggaaaaata
    63541 tccccaaagt tgttccattg tgtggaaaac ccgccgaaag tttttccatt gtgtggaact
    63601 tttcggcccg atttttccac agtgtggaaa atcccgtcca cgcgggataa acttttttcc
    63661 acagtgtgga aaacctccta accaactgcc tggacagtga ggaagctttc gccaaaaaat
    63721 cccgaatcct gagctctgct cctactcttg tccgccgtgc gcagcacata actactctct
    63781 gaaccctgac ctctgacctc cccgctgagc caaggccacg agccaacaga cgaaacggcc
    63841 aatagcggcc cagtcggcag tattcctcca atagctgcac aatggcggcg ggtgtggtat
    63901 ggttcggtga ctctgggaga aaaccggtga atttcaaatc aagaaaagtg gcgattgtgg
    63961 gtgcaggcgc ggtgggctcg ggctttgctt tcgcgctggc acaatcaggc attgccgagg
    64021 aaattgtttt ggtggatgcc aacacggatt tggccaaggg gcaggcgctg gatttggcgc
    64081 acggcgcgcc gttttatcga actgtatcca tctatgccga taccgcgccg caggcctatc
    64141 gcaacgcggc gattgtggtg attaccgccg gcaaggcgca ggccggcccc agccaaagcc
    64201 gcttggagct gatgcaaatc aacgccaaaa ttgtcaccag cattgcgacg gatatccaga
    64261 tggtgaaccc ggaggccatt gtgctggtgg ccacgaaccc ggtggacatc gtcacccacg
    64321 tcgcgcggtt gcgcacccaa tggccgcgcg gacgcattct gggttccggc acggtgttgg
    64381 actccgcgcg cttccgctgg ttgttggcgg agcattgcgg ggtgtcgccg caaaacgtgc
    64441 atgcctccat tctcggggag cacggcgata gcgaactggc ggcgtggtcg ctggtacaca
    64501 tcggcggcct gccgatggat cactattgct cggtctgccg caagtgcgac tttatccatc
    64561 gccggccgca aatcctcgag caggtacgac gctcggccta ccacatcatc ggctacaaag
    64621 gctccaccag cttcggcgtg gcgatggcca tggtgcagat tgtgcgtgcc atcctgcgca
    64681 atgaacggcg cgtgctgacg gtatccgcgc cgctggcggg cgagtatggc ttgaacgacg
    64741 tcacgcttgg catcccctgt gtggtgggcg ccaatggcat tgaggaagtc atcgaagccc
    64801 agttgactcc ggaagaaaac caggaattgg cccaatcggc ccaagtgctc aaggacagct
    64861 tggcgctgct ggaaacgagc ccggcataac ccgcgcgcgc ctcaatgggc ggcgatccac
    64921 ggaatcgcca caaatgtgcc gatgctgctg ccgatgttgg tcatcaccac cacgagcaga
    64981 atccgcacca ccggattgcg gataaagttc cggaaactct ccatggccgc cggcaatgat
    65041 tcaaaatccg ccacgttcgg cgggcggagc caggcttcca ccaagcccgc cacccatcca
    65101 gcggcaatcg tcggattcaa gctggtgatt ggcgccgcca caaaggcgga ggcaatcgtc
    65161 agcggatggg ccagcgtcac cgcggccccg atggccgcca gcacgccatt cacccccacc
    65221 caaatgagga tggaatcaat gcctttcgcg gccccgccat ggatgaagcc ccacacaatc
    65281 aaggccacga ccagggccgg aatgagccac ggccagaatt tggaccaacg ggtgcgcggc
    65341 ggcagacttt ccaattccgc cagcggcact tccgtttgca gcgcgcccac catgcccggc
    65401 acgtgccccg cccccaccac cgccacgatg gtgggcgcgg ataccgcccg caacttttgc
    65461 gcgagataaa cgtcacgttc atcaatcagc cgcttcttga tttgcgggaa cgccttgccc
    65521 atctccttca tcagggcttc cagttgatcc tgctttttca aagactcaat gtcttcatcc
    65581 ttgacatcgt ctccggagaa cagcgcttcg atcatcacgc ccagcaactt gacgcgctgc
    65641 cagaagccca agtgccgcca gacgcgcttg agggtgatgt ctatcgggcg gtcaatcagt
    65701 tcgaggcgcg cgccggacgc ttccgccgct gcggcggccg cgagcatttc cgcgccgggc
    65761 tccacttcca gttgcttgcc caggcggcgg tagaacgact gcatgatcag ttgcgccagc
    65821 aggaacatcg ccttgccctg gcgaatcact tgaaacaggt tggtgcgctg ccaggcatcc
    65881 ttgcgcacca tgccctggta gcgcgccggg cacagctcga tggccaccgc ggccggctgg
    65941 accgcggcaa tggtttcgcg cacatcggcc acgctctgcg ccgacacatg tgcggtgccc
    66001 acgatataaa tggttttgcc ctccaactcc actttcgtga cgctggaggg caaggaagag
    66061 cccgtcgtct gcatcgcaga aactactggg cgccgccctt cttgatatag atgtccgtgc
    66121 ggcgattctt ctgcatgttt tcttcggtgt cgttgggcgc caccgggcgg tcaaagccgt
    66181 agcccaccgc cgtgatgttg tccggattga tgccgctttg ctgcaccagg taatccttca
    66241 ccgcattggc gcgacgctca ctcaagcgct ggttatagtc gcgcttcgag gtcttgcgct
    66301 tgtcggcatg gccttccaca cgtacggtcg cgttgggatc gcgctgcagc accttcacca
    66361 ccggctcgat gtcagcaaag tactgcggcc ggatgaacga cttgtcatag tcgaattcga
    66421 tcagcagcgt gaacttctgc aagtcatcca gcggatccag cggattggtg ccgtcttcaa
    66481 tgacttcgtg gccatcgctg actccgccgc catccgtgtc aatgttcagc ggatcggtct
    66541 tgtaggtcag cacttccgcg ccatccttca agccgtcaaa atcgctatcc ggattcagcg
    66601 ggtcggtctt gtacttgaat acttcgtcgc cgtcggagag gccatcgccg tccgtgtcgg
    66661 gattgtaggg atccgtgcca atgatggctt catcgcggtc aaagaggccg tcgccgtcgc
    66721 tatcaatatc gccgccctgc gccacttgga acaccggggc cacgacgcgc tcggcgccga
    66781 agcgatagca cacgcccaac tcgacaaggg cggtgaattc ccagtccttg ccttgcgcgc
    66841 cggccacgac atcgccacgc agcgcccagg aatcgttaaa gtggtagaac aggccgccgc
    66901 cggcgaacaa gccgaactgc gtcttgccgt tatccatgtc atcgccaaag aaagtgagcg
    66961 ccggacccgc gcgcaagaag gggtccaccc ggcggttttc caacacgcgc agatgcagca
    67021 gggcatccaa gctcaggcga agcgccgtgg tgctgccatc gagaggcgtg gtgttcaact
    67081 tgctggcggg ccgggcggag agcgagggca tgaaatccac gccgccttcc acatcccacc
    67141 aggaattgag gctataaccc agcttgccca tcaggcccac gccgggcttg gcttcgacat
    67201 cgccttcgag gtggtaatag ctcacgccca aggtgccgaa ccacggagcg cgcgcaaaca
    67261 aatcgtcatc catcgccaca gccgaatagg tcaccccgga caccatcagg ccaaccgcca
    67321 acatccactt catcattttc atcatgagac gaactcctgt tcaaagattg cgggaacctt
    67381 accgacccca cccccttggg gcaaacaaaa aaaccgcggc tcgaaccgct ggctggcggc
    67441 ttgccaaatg caggacttct gcgtaccgtt ctgctgccat caaggcctgt cagatgagta
    67501 cgctttttga ccaatacggc gaaaattacg atgccgccat gaccaaatcc attggcttca
    67561 tgggtcaaag ccacgattat tacacgcagg ccaaagccgt tcatattctt tccattttgc
    67621 ggcggcattt gggcgccccg cgccaattgg cggtgctgga tgtcggcagc ggcgtgggca
    67681 agacggatcg ctttttgtcc ggtggaatcg gccacctgac cggggtggat atttccgcca
    67741 ccagcgtgga gcgcgcccga cgggaaaacc cgtccgtacg ctatgaagtc tacgatggcc
    67801 ggcaactccc cttccctgct gaatcgtttg ccgccgcttt tgtaatttgc gtgatgcacc
    67861 atgtggccat cccggatcgc gtggccttgc tgcaagaggt gcggcgcact ctcatccccg
    67921 gcggcctgct gatgattttc gagcacaatc cgctaaaccc cctgacccgg ctcgcggtag
    67981 ctcggtgcga gtttgaccga gatgctcaac tgctcacccg cggactttct gctcgattac
    68041 tgaagcaggc cgggttcact atcgccgaaa agcgatttat tctggtttcg cccttcgcct
    68101 tcaaaggcag cggctgcttg gagcgggctt tttgccaagc gccgcttggc gcgcaatact
    68161 acgtggttgg ccaaaaaacc gggtaatgcc accctgttta gggaatggcg gccatgcgca
    68221 actcacgcac caccagcgcg cccccggcca gcttcttgca cgccagttga tgaacgccgg
    68281 ggggcaagtc taccgtgaag gtttgcttgg ccagttggcc ggccggaaaa accagcggat
    68341 tcgactcgcc gatccgcagt gtcacgctgc tgccattgcc cgccgcggtc atttctccgc
    68401 ggaatgaagc cgtccgtccc gacacattgt gaacctccag cgtggcggcg tcctccaaga
    68461 tgcgatagtc ggtgaaatcc ccctgctgcc acggcttgaa cagccgccaa ccatccccaa
    68521 aaaacagaca ggcagtttgg ccgttggctt tggcgtgttc agccaattct tcgtgagtac
    68581 tgtagaacaa ttccggttca acacgcgaag tttcggtgta aaactcttcc attggcgcaa
    68641 agccggtatc acgcagccaa acgcctgtcg cattggtcaa caccacccga tgcgcgaacc
    68701 acttttccgg ccaggtccac accccgatgc ggtcggcatg ggttcgcgcc agccgcaaaa
    68761 aaccttgggc gccgttttgc tcaatgagcg tttgggtgcg cttgcgccag ttcagcgcaa
    68821 cgtattgctc atagggctca tccggcacag tgaaccccac tgtgacgtcg cggggcatgt
    68881 agcgcgctat ttcattccat ggctccagcc aacggtccac cagcacgaca tcgccgggcg
    68941 acatattggt gtccaaccaa tcgcgaatga gccgataggg cgttggtttg ccggccaagt
    69001 gcatgatgtg ccacacgggg gcagctaggg ccgccgcaat gagtaaaccc aaagcggcaa
    69061 gggtcagcgt ttggcgatgc cctccccatg ccgtcgccgc gcgttcgagc gtcatggcgg
    69121 cccagcaaat aaaagacggc aaattgacgg caaaatagac caatttggct ttacccccac
    69181 cacccacgcc aaacacatag gcataagacg cggccaagcc caaccaagcc aaccacgata
    69241 tggcggcata taacgaatcc gcggcggccc ccgtcttccg cgcgcgccac acattccacc
    69301 acaaggccgc cagcagcagc acaagcaacg ccccccccac ccagttggcc ccaccagcaa
    69361 aaaatggcag gacgcgtggt ccgaccaaac cgaaatcggc cccgatgtgc cccatcgggc
    69421 tcgcggcggt gcgctgcatt tcaagaaatg cgcggaaaat ccaccgcgac attcccaccg
    69481 ccaagccgcc ggccacccac gccatggcga tgctgtgccg tcgccgggcg gagctccaca
    69541 acccacgcca gccggtcaca aacagcagcg cccacgttat gccggccacg gcccaagcgc
    69601 tcatatgcgc aaggcaggcc agcagcgtcc acacggccca ctccccatat tggcgcgcct
    69661 tcagttccgt gccgcccctc aactgggtca tgaaatccgc gccacgcagc aacatgcccg
    69721 cggcgaaaaa catcgtcacc acgtaatagt aggcctcgcg tgaatgatag acgtgatacg
    69781 gcagcatcgc cagccagagc cccagcaaca gacctgcccc cagcccgcgc cgccgcagca
    69841 tccaccccgt gcagaacgcc acggtaagca aacccagcag cgcgaaaggt tcgcgggtga
    69901 aggcttcatc caccgtgcgc catggttgca acctcgccca aaccacggga atggaatcca
    69961 ccagtgggat ttgattaagc catggcgggt ccgtccacaa cttggccagc gtggtcgctc
    70021 cgccggcata gttgagcatg tttagttcgt cagagcgggt gggtgtatac cccaagtcag
    70081 cgaagcgcac ccagccggcc agcacgacga ccgcgcccca tgccagccac tggatgccgc
    70141 gccgcgtcat ggtctgtttc acttcgcgcc ggctgggtcg cgggtatccg accgcaccag
    70201 atacaacggc cgccgctttc cttcttcaaa gatacggctg acgtattgcc ccaacatgcc
    70261 gatgctcacc agttgcaccc cgcccataaa agccatcacc gcaatcagcg atgcccagcc
    70321 cttttccagg ctctgcggct gcacgatcgc cagcaccatg acataaatca cccacaaggt
    70381 ggccagtacg cacacgccaa accccagcac ggcgatccag cgcagcggcg aggcggagaa
    70441 agacgtaatg gcatcaaagg ccaaacgcgc gcttttccac accggatatt tggtttcgcc
    70501 ggcgagccgc gccgcccggt cgtattccac ggacacttgg cggaagccca cccagcacgt
    70561 cagcccgcgc atgaagcggt gcagctcgcg catctcgcgc agcgcatcca ccacgcagcg
    70621 gtccaccagg cggaaatcgc ccgcatccat cggcacattg gttttggcca gccgtcggaa
    70681 aacgcggtag aacgatgccg ccagaaactt tttgagccat gtttcgccgg cgcggctgcg
    70741 gcgtacggcg tacaccacct cgaatccctc gtgccatttg tccaacatgg ccgcgatggt
    70801 ttccggcggg tcctgcaagt ccgcatccat gatgaccacg gcatcccccg tggcccgatc
    70861 cattccggcc gtgatggcca actggtgccc gaaattacgc gataaacgca ccagccgcac
    70921 atgaggatta gtccgggtct ggtccagcgc ccattcggtt gagccgtcgg tgctgccatc
    70981 atccaccaac agcagctccc aattttcccg ccgtgccgcg aagaccccgg ccacccgctc
    71041 caacaaaatg ggcaggttgt ccttttcgtt caaaaacggc accacaattg tgatcagttt
    71101 tgcctcgctc atggggcaac atcctacccg gaccccgcgc ccgacgcaac cggcaagaaa
    71161 cccccggatc cgctgccaac cggaaaacaa tctcgctctg cgggaggaaa tggagtagtt
    71221 ttcccgactg tttgcttatg gatcacaccc ctgccaactc accccctctg ccaacggcgg
    71281 cagaccgcat gggcaccgag cgcgtcggcc ggttgctgct gcggttctcc atcccggcca
    71341 tcgtgggcat gctggtgcag gcgctttaca acatcgtgga ccgcatgttc atcggctatg
    71401 gcatcgggcc acaggggatt gccggcgtga cgatcagttt tcccctgatg atggccatgg
    71461 tgtcgttctc tattttggtg ggcgtggggg ccaatacgct gttcgccatc cggatgggcg
    71521 aaggccggcg cgaagaggct gaacggattc tgggcaacgc cttcggcctc ctattttttg
    71581 tgccgctggt ggcctcactg gtgggactgg cctacttgga tccactcctg cggctggccg
    71641 gcgccagcga tgaactcctg ccttacgccc gcgactatgc gcgcatcatt ttgggtggca
    71701 ccgccctcac gaccaccggc catggcttgg cgcactttat ccggtccgat ggtcgcccat
    71761 tcatttccat ggcggccatg ctgattggcg ccattaccaa caccattctg gacccgctct
    71821 tcatttttgt ttttcattgg ggcatgaagg gcgcggcctg ggccacggtg ctggcacagg
    71881 ggctctcatt cgcgtggtgc tttggctact ttttgcgctc ccgcaccggc acgcggctgc
    71941 ggcggcataa tctgcggctg cgaccccgtg aaattgtctg gccgctgctg gggatcggct
    72001 ttacgccttt tgccatgcac ctggccaaca gttttctgaa tgtaatcctc aaccgtggct
    72061 tgcgggaata tggcggggat gatgccatcg ccgtgatggg catccttgcg gcttacatga
    72121 gcattatttt catgccggtt ttcggcttgg cgcaaggtgc ccagccgttg atgggatata
    72181 attacggggc gcagcaatat gcgcgggtac gccgtctttt ccaaatttcc gtgctggtgg
    72241 ccacggggtt tatggtgatg ggctggacgt tgagtcagct tttccccgtc cgtattctgc
    72301 ggctttttgt cccggcagat tcagctttga ttcccctcgg tcgccacgcc atgcgtgtgt
    72361 tcacactggc ttttcctatt attggttttc cgattatggc cggtcaattt ttccaagcca
    72421 ttgggaagcc ggtcaaggct gccctcattg cgctttcgcg tcaaatcctc ctttttatcc
    72481 cgtttattct aatcttcccg ctgttctggg gtttaccggg tatctttttc gccgccccga
    72541 cttccgatgt catggccacg gccattgcca ttccattggt ctggcgccaa ctacgcttgc
    72601 tacgccgttc acccctcaag gagccgcgcc atgaagacgt gtgatacttg cggggaagaa
    72661 atcgcgccgc aatccagcat ctgcccctac tgtgaaagtc cgcaagcatg gagcccgccg
    72721 acaactaacg gtccggcgat caaaacaatt aatctggaag ccggcctgcc aacagtggaa
    72781 gaagcgctgc gccgcctcgc ggcccaactc gatcgcgcac gtctggaagg cgtgcgcgtt
    72841 gtccggctga ttcatggctg gggttccgcg gcgggcggcg gcgggcgaat ccgcgcggcc
    72901 gtacgccagt ggctgcagca agaggctgaa gcccggcgca ttcatttctt cctgcccgga
    72961 gatcacttca ccaacaccac gccccgcgga cgtgatttct taagccgtca ccccgccctg
    73021 cgccaatcca tccgtaccga tcgcgaaaac cccggcatca ccttcgtcga gccctgacct
    73081 cgcccgcccc tttcacaaac taacgaaaaa cgccgcggga aacccgcggc gtttgcgaaa
    73141 tgcattccta acgcgatacg ttacggagtc ggtgtaggcg gcggagtggg cggaggcggg
    73201 ctatccggaa ttatgccgga gccatcagca atcggcccgt cctgggagaa tgcggtatcc
    73261 gtcgggcggg tatccagtac catggccggc gcggcgccct gaatggtatc ggcgcgcgga
    73321 cccaaaaccc aagtgcccag catgatgcga tcacgaagaa cattgttgac ctgatagcct
    73381 tcaaaggtgc ccacaatcgt cgagccattg ctgccggtgc attcatacga agccacaacg
    73441 cggtcagtct tgtccgcacg cggatatgtc gggccgttgg tgctggctgg cgtgggcaac
    73501 acgccggatt cggactgaat ccacttcatg tagccggtgt acaagttgcc gcggtcatcg
    73561 cgcatttcca gattttggcc gcggtggaag accgcaatcg aatggatgcc gcccagcttg
    73621 atcacatagc tgttgccggt gaacggagtg taggtgccgc tgaaattgac ccagttgtag
    73681 gtgtcggtcc agctcgtggc ctcgttgcct gtatcccagt tgcagccgac catggccacc
    73741 gccgccaccg tcgccagaat tccgatcaaa gccatccgtt tcatgccgtc tccttgctag
    73801 atgctgctat ttgttgcgac ttatacccgt ttgcccgcgc tgccgtcaac gggtatttcc
    73861 gaaagcgcgg tcagcagggc atccatttcg gcatcggtgc cgatgctgat tcgtaaaaag
    73921 cggccggtgc gcgcgccggg gaaatagcgg accaggatat ttttttgccg gagggcggca
    73981 aacagttgct cggcgggcaa accgtccggt ggctgggcaa aaaggaagtt ggcggccgag
    74041 ggtacgacat aaaacccacg ttgttgcaat gccgcggtta cccgttcgcg cgtggcgaca
    74101 atgcgccgcg cattggcttg catccactgc acatcattca acgcagccag cgcaatcagt
    74161 tgggccagcc ggtccacgtt gtaggaatct tttaacttat agagtgcggc aatcaattcc
    74221 ggcgcgccca aggcaaatcc cagacgcagc ccggccaacg agaacgattt ggataacgtc
    74281 cgacacacca gcacattggg caattcacgg gccaaggtca ggcagtcgcg tccccccgca
    74341 aaatccacgt aggcctcgtc caccgccacc acgcccgtaa aacgttcgct gaactcgcga
    74401 atcgcctcca ccggataaaa cacgcccgtc ggcgcattgg ggttggccac ccaaaagagc
    74461 gtggccgcca aatcccccgg cggcgccacc cacgagaaat catccggcaa cggcacttcc
    74521 caaaatccaa cttcttcggc ggcggccaac accggatata aggaataaga gggcgcgaag
    74581 gaggccacag tcccgcccgg ctgggtgaag gcccgggtgc agaggcgcaa tacttcgtcc
    74641 gacccgttcc ccacgaacac ctggtcggga gcgcacccgt acagggatgc gatccgtacg
    74701 cgcagcgcgg aacacaccgg gtcggggtaa aggcgcagcg attccgccgc cacggtgcgc
    74761 agcacttccg tcacggcggg gctgggcgga tacgggttct cgttggtgtt gagcttgatc
    74821 aagccgggaa cttgcggctg ctcgccggga gtataggcag tcaactgttc aacggtttgg
    74881 cgaatacgcg ggttcatgtt gttccattca cttctggttc agtcttgccc aatccgtggg
    74941 cagacgcaag cgctttgcaa aacgaaaaag gacgcccggg ggcgtccttc ttggcaacca
    75001 tctggttggc ttccttacca gcggtaatgg gcgaacgcct tgttggcggc ggccatttta
    75061 tgcgtgtcgt cgcgcttctt aatggcattg ccctggtttt tgaaggcatc aagaatttca
    75121 agtgccagcg cgcgctgcat ggaggcgccc ttgcgcccgc ggctgtaatc cacgatccac
    75181 cgcagggcca aactggtctg gcggctggcc ggaacttcca ccggcacctg gtaagtggcg
    75241 ccacctacgc ggcggctctt gacttccacc ttgggcttaa tattttccaa cgcctgttgg
    75301 aagacttcga tgggatcctt gccctcggtc tgttccttga cttgatccag tgcaccgtac
    75361 acggtgcgtt gggccgtgga cttcttgccg cggctcatga tgatgttggt caggtgggtc
    75421 accagcacgc tgccatagcg cgcatccggg gcaaactcac gcgattctgc tctacgccgt
    75481 ctcatgctgc ccgcctccta ctttgccgcc ttgggggtct tggtgccgta tttggaacgg
    75541 ctgcgccggc ggctctcgat gccgaggcaa tccaacgtgc cccgcacaat gtgatagcgc
    75601 accccgggca gatccttcac ccggccgcca cgcaccagca ccatgctgtg ttcttgcaga
    75661 ttgtggccct cgcccggaat ataggcggtg acttcttcac cgttggtcag gcgcacgcgc
    75721 gccaccttgc gcagggcgga gttgggcttc ttcggggtca tggttttgac caacaggcac
    75781 acgccacggc gctgcgggca ccgctgcaaa gcgggcgact tccgctttga actcagcggc
    75841 tggcggcctt tctttaccaa ttgattaatc gtcggcatat cgttcttttc gttccttcca
    75901 cattcggtct ttttgacaca aagtagcgcg cacagtacca agaccccccg ccgctggcaa
    75961 cccccttgtg catctttttt gcattttttg cggggcataa aaaaacgtgc aatcggagcc
    76021 gtgacagcct atggttgccc aagtcgaaag gaaaccactc tctgcgacag gatggatgag
    76081 catgaaccga atcaccagcg cacttctgat caccctggcc ctggcggttg ccgccacggc
    76141 tcaattgccc agccccagcc gcccaggccc cctgcgggtg gccatgctgt cggatggcgg
    76201 cagtttcagc gatagctcct tcagtcagaa ctgccgcgag ggcctcgaag aactgcttta
    76261 ttccggcacc cccatttatg tgcagtttgc cgaaccgatc acccccgcca attttgagcg
    76321 caaactgacc gcgctggctg aacgggacta ccgccttatt ctgggcattg gttatcgcat
    76381 ggcagatgcc gtgcaaaacg tcgccgcctc ctacccccgc accctgtttg ccagcgtgga
    76441 tggcgacttc gaggacatcc cccacaacgt ctgggtgttg accttccaag tagacgagtg
    76501 cgcgttcccc gccggctatc tcgccgcggc ttgggctgat ttgcaggatc cgcaggaccc
    76561 ggccattggc tgggtgggcg gcgtggattg cgaaagcgtc aatcagtttg tcgtcggctt
    76621 ccggaatggc gcccaacact ataacgagat caaaaaccgg caggtgcgca tcttgggcga
    76681 ttatgtcgga tccttcggcg attttgccgg cggcaagaaa ctggcgctca aactgctcga
    76741 cgacggggcc gatgttattt tcggcgtggg gtccatttcc ggcaacggcg gcattgccgc
    76801 ggcaaaggag cgtggcaagt gggccatcgg cgtggatacc gaccaataca acacgctggt
    76861 ggattatcgc gatgtgctgt tgacctcctg cctcaagcgg atggatcgcg ccgtgaaaca
    76921 ggtggtgatt gccgctttgg aaaaccagtt ctggggcggc tcgcgctaca tcggcaacct
    76981 gaccaaccat ggcgtcggct tggcttcgta tcacgattac gcggataaaa tccccgagcg
    77041 cctgcaaaag gaaatcgtgg atatccagcg gcgcattctg gacggctcga tcgaaactgg
    77101 ttggccggca aaacccgccg tgcagaaaaa agcgcgcgcc aaggccgccg aaccggccgc
    77161 ggccgcagcc gccgcggccc catgattacc ccggcgttct cccgtttgtt cggtgcgcgc
    77221 cgcaaggtgg tcatcggcat gatccatgtg cgcccgctgc cgggcacccc gcgccatgcc
    77281 cacgattggc cggcgatcat tcaaaccgcg ctggccgagg ccgaaaccta tcgcgccggc
    77341 ggcattcatg cgctgatgat cgaaaacatg catgatattc catacgtcaa aaacccgggc
    77401 ccggaaatca ccgccgccat ggccgtgatt gcccgcgaag tcaaacgcgc ccaccccgcc
    77461 ctgcctttgg gcatccagct tttagccggc gccaatcagg aggcgcttgc cgccgctgct
    77521 gctgccggcg ctgatttcat ccgggccgaa ggctttgtgt tcggccacgt ggccgacgaa
    77581 ggctatttcg attcctgcgc gggcgccttg ttgcgctatc ggcggcacat cggtgcggaa
    77641 tccctcgcca ttttcaccga catcaaaaag aaacactccg cccacgccat cacggccgat
    77701 gtggatattg tgcaaaccgc ccatgcggcc ggatattttt tgtcagacgg gctcatcctc
    77761 acgggggccg ccaccggcga agccgccagc cttgatgaat tgcgcgcggt ctatgccgcc
    77821 acccaattgc cggtgtttgt tggctcgggc atcaccgcgg acaatgtggc cgattacctg
    77881 ccctgcgccg atgctttcat tgtcggctcg cacttcaaac aggacggtta ctgggaaaac
    77941 ccgttggacc ccgcgcgcat ccgccgcctg ctggcggcag tgaactgatc gccccgccgg
    78001 ctcgcgcgcc cgcgcgcgcg agtaccaatt attgaaacgc cgcccagtgc tcgcggcgga
    78061 tgcgctccat gcgcaggcgc gcctcaactg cagccggcac gccggcagcc gtcacccgcc
    78121 ggtaaatgtt ggccgcttct ttccactgcc cagcagcttc ctgcaaggcg gcggcagcaa
    78181 atgccgcgcg ggtgaaccac accgcggctt cttccggtgg atgttcttct tgcaggaagg
    78241 cgtacaccac ctccatgtag cggtccagcg ccgcggtggc acgccccatt ttttccaggc
    78301 agcgcccgac cttgaagctg gcttgaatgc gcagatcgaa gggcgtgtgg gcatcgcgca
    78361 atacctcgcg atacgtcaac aaggcctcct cgtagcgcgc cgggttttcc tgcccgagcg
    78421 tgtattggca gtcgcctttg cggcccagcg ccacgatggt ctccacttgg cgcggaaact
    78481 ttttgatgac ttcatcaaac gccacgacgg cggcggtgaa ttggcccagt tcgctgagca
    78541 cgtcgccctg cgccagcagc gtcgccggca gccgggggct gtcgggataa tccttcatca
    78601 gcgcgttgta atgctcgttg gcgatcagat aattttggct ggcggtcgcg gcgcggcccg
    78661 cccaatagcg cgcatcggga gcgcgcgcgc tttccggatg ctccaccgcc acttgggcaa
    78721 agcgctgctc cgcttcggca tagttgccgg cattgaaggc atattcgccc agccagaatt
    78781 gcacgtcggg cagaaagcgc gacgcaggat attgcgccag aaaatcttcg cacacctgca
    78841 aggcctccgc gtccttgccc agcaaataga aacaccaccc gcgcatgaac atggcctgcg
    78901 cggcggccgg ggcggcaggc tgggtttcgc tgatgcgcgt aaagtcatcc aaggccgcct
    78961 ggaattggcc catgcggtag cggatcaacc catgtgccag cagcgcgtca gaacagtccg
    79021 ccccagccgg gcaggattcc gtcacgcgcc gatattgctc cgcggcggcc tccaacgccc
    79081 ctcgttcctc atataaagcg cccagccgca tggtggcgcg acgcgccatg tcggccgatt
    79141 ctgttgtgcg acttaaatcc agcagacggg tttcgccagc ttccgtctca cccaacgcca
    79201 attcggtttc ggccaactga tactgcgcca gccgcgccgc atcgctgccg gagctcgtgg
    79261 cggccaacgc cgccgcgtag gcctctttgg ctttggcaaa ctgccggctg gcaaaccggg
    79321 cgtcgcccaa ctggtgcaac cgtgtcgcgc gttgagtcgc atcggtcgtc aacgacaacg
    79381 cgcgctccaa tgcggcggcg gcttcttccg ggcgggcggc cgcccacaag gcgttgccca
    79441 gccccgccaa cgcccgcgcg gtgccctcgt tgccgtcaaa agcggccaac cacgcctgat
    79501 attccgcaat ggcttcagcc cattgcgtcc gctgcgccag ccattcggcc aattgcaact
    79561 gcaaacgcgc ggagagcttt tcatccggca cctgcgcggc cgttgtccgc atcagccgga
    79621 ccccttcttc ggtttgaccg gcttgcagga gaatctcggc tttgacggct tgcgcggcca
    79681 aacgctgcgc ggggtccgtc gccagcgcga ggctccggtc cgcccacgtg atggcttccg
    79741 ttgtattgct ggctgccgcg gccaaacggg ccatcaccaa attggcggcg gcacgggtgg
    79801 cgaccgcgac attggtattg gccaacagcg cttgcgcctt ggccaccgcg ccggtttgct
    79861 cgccttgcgc caactgcatc tcgatgagtt gcaaggcggc ttgttcgctc caggcttgtc
    79921 cggcgaagtt ggtttctaca aacgtcagcg cggtgcgcgc cacgtcgcgt tgtccagccg
    79981 ccagcgcccc ggcgcctacc gccagccagg cttgcggttc ttcgctctgg ccggcaaaag
    80041 cctcgcccaa cgcgcggcac atggtttgcg cggcgggcca atcttgttgg gccaccgcca
    80101 actgcacctg caaaagcaga gccgccggca ccgtcggctg cccggcatac agtttggcaa
    80161 aatcggccag ctcggccgcg gcttcggcaa tttggcccaa ggcttgatga acctgcgcgc
    80221 gcgtcaaagc gtactccgcc tgcatggcct gcgcccgcgc caagccgcca tgctgcgtca
    80281 aagtggccaa cgcttcctgc gggcggccgg cggccaattg cacccgcgcc agccaaagcg
    80341 cgccttccgc ctgccggcga cggtccggcg cgccatccac caactcctcg agcttgcgtt
    80401 gcgccagcgg atacaaacca tcctcccagg cggcccgccc catcaccagc agtttgcgcg
    80461 cttcttcaat ggtcagcttg gcggtggcca attgttcatc ggcgtcgtcc atcgccgccc
    80521 acgccgcccc ccacggcagc aggatcgcca acaccaaccc tagccccagt ttcttcatga
    80581 cttgctgttc tttttcgtcc gtcgcggcat cttcagaagc ggccgcaaat actcgccggt
    80641 gaacgaggcc ggatcggcag ccacttcctc cggcgtgcca caagccacca cgcggccgcc
    80701 ggcgccaccg ccctccggac ccaaatccac aatgtagtcg gccgttttga tcacgtcgag
    80761 attatgttca atcacaatca acgaattacc ggcatcgcgc agccgcaaca acacctttaa
    80821 caaccggtcc acatccgcaa aatgcaggcc ggtggtgggt tcatccagca aatacagcgt
    80881 tttgccggta tcgcgccgcg cgagttccgc gctcagtttc atgcgctgcg cctcgccgcc
    80941 ggagagggtc gtggccggct ggccgacctt caagtagccc aatcccacct cgcttagcgt
    81001 ccggaacttg cgcgcgattt gcggcatggg ggtgaagaac tccaacgcct catcaatcgt
    81061 catgtccaac acatccgcga tgctgcgtcc atgccatttg acttccaacg tctcctgatt
    81121 gtagcgccgg ccgcggcatt gttcgcacgt cacatacaca tccggcagga aattcatttc
    81181 tattttcagg atgccgtcac ccttgcattt ttcgcaacgc ccgcctttca cattgaaact
    81241 gaaccgccca ggcttgtagc cgcgcatttt ggcggcgggc aactgcgcga acaaatcgcg
    81301 aatcccggcg aacgcgccgg tatacgtggc ggggttggag cgcggcgtgc gcccgatggg
    81361 ggcctggtca atcacgatca ccttgtccag atgagtcagg ccattgatcc gtttgtgctt
    81421 gcccggtttt tccttggagc cataaaactt gcggaataac gcgcggcgga ggatgtcatc
    81481 caccagcgag cttttcccgc tcccggaaac acccgtcacg cacgtgatcc gccccaatgg
    81541 gaaacgcacg tcaatatttt ttaaattatt ttcggccgcg ccaatgatct ccagccagtc
    81601 cccgcccggc ggcagccgtt tggccggcac gctgatttct ttttcaccgt tgagaaactg
    81661 cgcagtcagc gaggttttgg actgcaacaa ttccgggacg gttcccgcaa aaaccacttc
    81721 gccgccctgc acgcccgccg ctgggccgag atcaatcacg taatcggcgg cgcgaatggt
    81781 ttgttcgtcg tgctccacca ccaccacggt gttgcccaaa tcgcgcagcc ccttgagggt
    81841 gcgcaacagt ttttcgttgt cgcgctggtg cagcccgatg ctcggctcat cgagcacata
    81901 cagcacccct accaaccccg cgcccacctg cgtggccagc cggatgcgct gcgcctcgcc
    81961 gccggacaat gtgccgcttt cgcggtccaa gttcagatag cccaggccca catcgcacaa
    82021 aaacatcagc cgcttgcgga tttccttgag cacctcgccc gcaattttct gctcctgctc
    82081 gggcagcggc aaatccgcga accacgccat cgccgcgtgg gcggagagcg ccatcacatc
    82141 catgatgttc tgccccacta catagcaggc caggctttcc ggccgcaacc gccgcccacg
    82201 gcaggcggtg cagggcagcc ggctcatgaa gttggtcagt ttttgcttca tgtagtcgct
    82261 atccgtttcc tgatagcgtc ggttcaggtt cgggatcacg ccctcgaaag gcttgcgata
    82321 tttgcgccag gaaccacccc gccaaaagcc cagcggaatt tcctcgtcgc cggagccatg
    82381 caaaatgatc tcgcggattt ttgcgggcaa gtcgtcccac ggcgtctcca tgctgaatcc
    82441 gtagtgcttg gccaccgccc gcagcaagcc cttgcgataa atcgcctcgc gccgtccgct
    82501 cgggcgccac cccgcaatgg cgccattgtc cagcgacttc gtaccgtccg gcaccaccag
    82561 ctcttcatca aacaccagca tttgccccag gcccaagcaa gttggacacg ccccgtatgg
    82621 gctgttgaaa gaaaaattgc gtgcttgcag ggcatcaaaa ctgatcccgc aatccgggca
    82681 agcgttcttt tccgaaaaca tctgggttgt ccacgccgtg gcgccccccg gctcttggaa
    82741 caacgcgtgc aggatcccct cgccctgctt cagcgccaat tccacggagt cggtcaaccg
    82801 gccgcggatt ttatcgctga tcaccagccg gtccaccacc gccgcgatgg aatgtttttt
    82861 ttgtttgtcc agtttcggcg gcttttccag ctcgtagatt tcgccatcca cctgcacccg
    82921 ggtgaagccc tgccgccggg cattttcaaa cacctccgca tgttcgccct tgcgcccctc
    82981 caccagcggc gcgagaatct gtaccttggt gccggcatcc ttcttgagca gcaggttgac
    83041 gatttcttcc gcgctttgcc gcgtgatggc ccgcccgcat tggtggcagt gctgccgacc
    83101 gatactggca taaagcagac gcagataatc atgaatttcg gtcgtggtgg ccacaatcga
    83161 gcgcgggttg gcgcctgccg aacgctgctc gatggcaatc gccggcgaaa gcccctcaat
    83221 gcggtccact tccggctttt gcatctgttc gagaaacatc cgcgcatagg ccgagaggct
    83281 ctccacatat ttacgctgac cttcggcata aagggtgtca aacgccagcg aagatttgcc
    83341 gctgccgctg aggccggtca ccacgcaaag gctgccgcgg gggatgcgca cggtgatatt
    83401 cttcagattg tgctcgcggg ccccctccac caaaatgcag ttgttactca tgatccatgg
    83461 ccttctaaaa agtgacgggt aaacctacca cgcccccccg cccctgcaaa tccgcaaaat
    83521 gccgcaaagc cctgcgcgtg actggtcatg agtccggact tgcgccaccg ggaatgcccg
    83581 cctatgattc cccgcatgtt atcccatgat ggcagccaaa atgggtcgcc ttttccggcc
    83641 gatccgcccc cggctattcc gccgcgcgag caccagcggg cgccccgccg tccgtggccg
    83701 ccccggcagc cgaccgcgcg tcgccatacc atccaaatgc tgatggccgc cgggtttggc
    83761 gtggtcttaa tgttcggcct gctgatgctg gccgtacgct atgccgaaag cgattggcac
    83821 aagaaaaaca tgcaggcccg ttcccaccat ggcacggccg ccacgccaac ggcagccgcc
    83881 accaaggcca cagccgcctg ttggaaatat accctgcccc ctgccgccgt gccgcacacg
    83941 ctggaacggc tgggcggcga tgccatcctg ccgaatcatt ggttggttct cggtcgcggg
    84001 ttgtccaata aaaccgatcc caaactcacc ctccgggccc tgtggctggc catgtccgtc
    84061 agcggcgagt cggtgggcat gaataacgac ttcggcgtgg cctatctcga acaaaagcgc
    84121 attggcgagg cggtcactca attccaagcc gccctgcaat tgcatccggg atttcctccc
    84181 acgctgttca atctggcgct gtgcgccatt gcccagcgca acccggccga ggccagccag
    84241 tggctggggc gctacctcgg ccagcgcccc accgatgaaa ccgccctgcg tttgcaggcc
    84301 acgctgttgg tccaaaccgg gcgcgctgac gaagcgctct tgctgctgga aaagtttttg
    84361 cgcgatcaac cgccaacgca gccgctctat ctggaggccg cgcaaatcgc cgcgcgcctc
    84421 aggcaaaacg ccaaggccct gcgctacttg gaggtggcgc tgaatggcaa tcccatacaa
    84481 gccgtggtgc gcacctatca atccgccctt ttccgcgaca tccggctctc cggcgatggc
    84541 gatgatcttg ccgcccgcct ggccaaaagc gcgcggattg cctacagcac gccggtgagc
    84601 gaagataccg ttctccccat ccgcgtgacg cccgacgccc tgttgcgctg accgcccgcg
    84661 cgggcggaca ttgcccggcg attccgcccg cttcaattgc gagtggcccg cagcgtgtac
    84721 ctcatggcgg gccagcgcgc cggccggcca tgccagtcga gttcgatcgt cccctgttga
    84781 tccgtccgcc aaattgcgat gttgcgcgcc gtcaaacggg ccatgacttc ttcatccggc
    84841 aaattctttt gcaagtgctc gccgccgctg atcacgacat cgcgtgggcg caccgcatcc
    84901 agccaaggcg ccgagttggc ctgggcatcg ccatgccgcc cggccaacaa taccgaacca
    84961 gccacggcgg ccccgccacg cgccagattc ttttcctgct cgccgcccag atcacccgcc
    85021 acgatcacgc tgacgcccca ccgcgccacg cgcagggcca atgccgcatc atcggcgcac
    85081 gtcattttca tttccgccgg cggccacaat acttcccacc acaagccgcc cggccagtgg
    85141 ccgcaatcgc cgcgctgcaa acgccgcacc ggaatgccgg cggcagcggc ctccgtcaaa
    85201 atactgttca tcagcggcga cggccaaatg gcttgcggca gccaaagttc ggccacgggc
    85261 agttgcgcca tcacggcctt ggctgcgccg acatgctgcg catcggcgtg cgacagaatc
    85321 aacgcctgca aacggttcac gccctcgcgg cgcaaggcgc gcatcaaatc atcggcgcgg
    85381 aatgccgcgc ccgtgtccac caaataagct gcgccgcggg cttgcaccag cgtggcattg
    85441 cccgacccca catccagcac aaccaaccgg caccgctgct gttcccacac cccccacccc
    85501 gcggccagca gggccagcgc cagcccgccc agcaacatcg cgccccgcac gcgccgcgcc
    85561 atgatggcca ccacaaccaa aaggccatac cacgccgcca tgccccacaa cggcggcgac
    85621 cgcatgaacc agtgcccgcc gggaatggcg gccgcccact cgatgcactt ggtcaaaaac
    85681 aacgccaccg ccaaggcggc atgattgaag atcccggcca tcatggcgct caccggcgcg
    85741 aacagcaggc acaatacgcc cgccagcaaa atgacgaacg cggatggaat gaccaacaga
    85801 ttcatgacca gcgccaccgg cgaaaccaga ttaaagaagt acaacgtcaa tggcgcggtg
    85861 gcgatccaag cgccgagcgt cacgttgaag gcccgcacac cgttgaaggc ggccgcgcgc
    85921 cagcgccgct tgggcggtgg ttcttcgccg ggcagttgcc acgcatcgcg ccggaaccag
    85981 cggtgcatgg cgctgtccag caccggggga atggccagca ggccggccac cgccgagaag
    86041 gagagcagga aaccgaggtc acccagttgc gccggcgcga ataacagaat caccaaggcc
    86101 gccgcaaaca gtgtcgagat ggcatcagac cggcgcttga aaaaaggtgc ggccagggcc
    86161 agcagcgcca tgatgcacgc acggatggcg ctggtcgccg cgcccgtggt gatggtgtac
    86221 agcaccagca acggcgccaa aaaaggaaac caccaggtca ggggcagccc catggctcgc
    86281 aaggtcccga taatcaacac ggtcaccatg cccacgtgcg ccccggaaat ggcaaaaata
    86341 tggacggtgc cagtggccgc gaaatcgcgc cggagggcac gcggcaaatc ggcgcgatac
    86401 cccatcagca gcgactgcac caccccgcgt tgatccggaa agtcctccag cccgcggccc
    86461 aggatctcgc ggcacgcatg acgctgcttc atgcaccatg ccactagcgg atttccctgc
    86521 ccgtcgtcca caaacgtcag ccggtcggaa tccacgatgg cttcattttc cggcagcgtg
    86581 aacaacccgg tacgccgggg cacgttcgcc cgcaccaagc cgcgcattcg ccagcgttcg
    86641 ccatagcgcg gcaaacggcc atgtgccggc gcaccgccac gaatgacgat ccgcagccgg
    86701 tcgtccaccc gccgccactg gccgtcacgg ttgagcccct ccacgcgcgc catcatcacc
    86761 cacccatccg cccgcccggc gcggggcggt tgcggcaccg cgtcttccgt ggccaccgcc
    86821 acaaaatgca gatattccat cgggcgcgcc atgtgtgcgg ccaacgacag cgcgctacgc
    86881 ccgcccgtcg ccagccgggc atgggccgcc accaggcaaa acgtggccac catcaacaac
    86941 ccgactgacc accgatgctg caccaacaca aacagcggta gcaaaaggaa tgcacccacc
    87001 cccaggaacc acaccgcgga gactttcgcg cagatgccgg tggccgtgcc ggcaattacg
    87061 gccagcgtaa tgcccaccaa cgcgcgacga cgcggcgggt gcgcagcacg aggcatgctt
    87121 tgcagttcat cgtcaatcca cgattccacc cggacggctc ccgcgcttca tgcggtctct
    87181 tgtaccccac ttgcactcca cgccacaagc ggcaactcgc cgcccacagg atgagttgca
    87241 ctgcagaagc cgttttgtct tgaaattatt gcggcaaatc ccatggttga gttgtgggct
    87301 gttgccacag gagactgaca atggaaatgg gtacgtcatt cggcatggga aaatatcggt
    87361 ggcggctggg ctggggcgcc ttggcgtgca tcctgctgat tccctgcgcc tggggcgcac
    87421 ggccggaagc ctacttcagc ggcacgcggt cggatgccaa cgtctatgtg gctcccgtgg
    87481 cctcctcgat ccacaaaatt gccatcttgc cgcttaaggc gccgaccgag ttgatcggct
    87541 cctccgtggc cgatatcgtc gtcaccgaaa tgatgcgcgc cggccgctac acgctggtcg
    87601 agcgcggaca aatgagccag gtgctgggcg aagcagaatt ggccttggcc ggcttgtcgg
    87661 aatccgcggc cgtggaagtc ggcaaaatga tgggcgcgga cggcgtcgtg attggcacgg
    87721 tggatgaata cggcatggtc gcccaccgcg gccgcagcta tccggtggtc ggcgtttcgt
    87781 tgcggctcat tgattgtgat tccggccgcg tgatgtggag cgtcggccac gcccgccgcg
    87841 gcaatgatcc cgcggggacc ctgtccagcc acgcgcgggc cgtggttcac gaagtggtgg
    87901 ctgcggtcgt ccagaattgg cgcgtgcaac gtcaagtggg ggaaaaatat gacgacgccc
    87961 gcggcactcg cgaccgcccg gatgatttgg tcggcatgca tggcgatccc gtggcgccaa
    88021 ccgcgcccgc cttcgagccg ccgcaagcgc cggactcggt ggccatttcc gattttggcc
    88081 tgcgcgaggt gcgcttgcaa tggactcccc ccgccgaccg ttccattcaa taccgcgtgg
    88141 agcgggcgga aagccccgcc ggcccgtttg cggttttggc cacgctggcc gctgcccgcg
    88201 gcgcatacgt ggacagcggc accgcgcgcg ccccgctgca agatgccggc acatattatt
    88261 atcgcctcac cgcgatcgga cccgatggcc aggaaagcga gcccggcggc gtgctggaaa
    88321 gcatgactgc accaccgccg gaaccgcccg cggagctcca ggcgcttacc cccgccgccc
    88381 gcgcggttca actgcagtgg gccgccccca gcagcgaagg cgtcacgtcc taccaagtcg
    88441 aacgggctgc ggccagcgat gaagtctttg ccctggtggg caccgttaaa accacgacct
    88501 accacgaggg gggcaccgcc gcctcgccgc tccgcgattc cacgccttac ttgtaccggg
    88561 tgcgcgccat caatcgcgtg ggcgccgttg gcgaattcag caagccggtc gaagtcatca
    88621 cccgcccccc acccgccgcg ccgcagaacg tgacagcaga aagccaacag gtgcgctgcg
    88681 tgccgctctc ttggcaggcg agccaagagg aagatgtcac gcattacatc atctatcggt
    88741 cggaaagcga tgccggcgtt tttacttcca tcggcacggt caaaggacgc gacaacacca
    88801 aatatctgga tggcggacgc gatcccggca ccctggaaga tgaccaagcc tatttttatt
    88861 gcatccgcgc cgtaaacgcc gtgggcgccg aaagtgacga ctccgaagtc gtgatggccg
    88921 gcacgcgccc cccgccgcca cgcatttcgc ggttggctgc gcaaagcgga ctgccgcgca
    88981 aagtggccct cacatggcaa ccatcggacg atgaaaaagt gatcggctat gacttggagc
    89041 gctccgaagc gggcggccct ttcgcgccgg tgggacggct cagcgggcga gagattgcgg
    89101 aatatgagga tggtgggggc gaactggccc gctcctggca ctccgggaat aaaaattcgc
    89161 tgcaagatgg catgccgtac acccaccgca tccgtgccgt caataccgcc ggcgcggtgt
    89221 ccccctgggt ggaaaccgcc ggcgccacga ccaaggtagt cccggccaag ccgacgggcc
    89281 tcaaagcctc cgagcggcgc gcgcgagtga tcggcttggt ctgggccgcc aatcccgaaa
    89341 aagacatcgc cgaatacgtc gtggccatgt ccccctttcc tgaccgcaac ttcaccgaaa
    89401 ccgcacgaat tccggccggc gccatgcgcg ggctaaagca ggaaaacctg ccgcccgatc
    89461 tgacgcgcta ttaccgcgtc aaagccattg atgccgacgg actcgaaagc gaatggtccg
    89521 atgtggtgtg cggcaccacc aaacccctgc ccgccgcgcc ggcggagctg gccgtggaat
    89581 gggaagaaga cggcgcgcgc ctgcaatgga caccgccacc ggagcgcgac attgtccggt
    89641 accgcgtgct gaacaaaaga ttgtttggcc aggatgaaat tagcacctgc accagcccgt
    89701 ccatcctgat cccgctcgcg acactcgccc cgaaaaaagt cttcatcgtc atcgctgtgg
    89761 acaaagacgg tctggaaagc ccaccctccc cccctctcga agcccgcccc ccgcgctgat
    89821 cccaacgcat cgccaaagtt ttccacgctg tggaaatctt ttttccacac tgtggaaaaa
    89881 ttcagggcgc ggggctgatt tttttccacg caatggaaaa agtttgagcg atttttccac
    89941 acaatggaaa ataggcgagt gccgagcccg agcccgcagg gcgatgggcg aacgcgagct
    90001 ctatagcgcc ggcgcagccg ggcgagcgag cgagccatag cgagcgcagc ggcaaacttt
    90061 ggcgagattt tccacacaat ggaaaaactt tgagcgattt ttccacgcaa tggaaaaact
    90121 ttgaggaaag tttatcccgc gcgggcggga ttttccacac tgtggaaaag tttgacggcg
    90181 cattttttag tttgccgttg acggtgggcg gaggggggaa tacaaacagc ctccatggat
    90241 gactttttgc cctatttgcg gcaggccttc cggcaggcgc acggcctgcc gctgctggtt
    90301 ccgccgctgg cggttccaga gccggcgtcg cggctgctga catgggcacg gcggcaccgc
    90361 atccgcaatt tgatccaggc agaatttcca acggcggacg agcccgttcc cacggcggcc
    90421 tatgggcagg tgcagcatac ggcgcgctat gtggccgaag cggagcgact gcatggcctg
    90481 ctggcgcatc ggctgcgctt tttggcgttg gtcaaggggc cgtcgctggc ggcgcaagca
    90541 tggccggagc cggggttgcg gatctatgat gatttggatt tcatgtgcca cccgcgcgat
    90601 tttcttcagc tccgcgtgat tctgaaaaac gagggctaca ttccgcgcgc gcgcgacgcg
    90661 cggcacttgg cgcatttgtg gtatttcggc tggggagtgt cgttcgatca tcccaacggg
    90721 cacatggtgg aggtgaacca ccgatttttc ccaccgcatt atccgtggcc ggtgcgccgt
    90781 aaccggagct ggcgcggcca gtttgcaccg caacggctgg atgaggcaga ggtgcaggcg
    90841 ccgacgccgg ctttgcattt gctgttgagc tgcctgcatg ccacatggca cggatgggcg
    90901 cgactggttt ggctggtgga tattgccggg ttgctggcgc gtcatccgga ggcgtttggg
    90961 caagcgcagg cgctggccgc gggcagccct tttgccgagt tggtgctggc ggtgggttgc
    91021 caggcggcgg aggagctgct tgggccgggg ctcgcgccga cggcgcgcga gttgccggcg
    91081 accagcgagg ccgaaagcgt gctgcatttg ctggatggca cggaggcgcc attgcgccgg
    91141 agccatctgc gccggttcca cgagacattg atgacgcgcg nnnnnnnnnn nnnnnnnnnn
    91201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    91261 nnnnnnnnnn nnnnnnnnnn gggtcgctga ttgacaacgt caaccaactc ggcaagggcg
    91321 gcaacctcgg ttactggggc aactggaaca gcgcctatac cggcgcacgc caaggcggac
    91381 gtctcgacct cgtcggtttc acctatcacc actcgctggc gccgctgctg cccaaggaaa
    91441 ttttccgcaa ggaattgcag attttcaagc aggcgtggtg gaaagcttgg ggcggcaatt
    91501 ccgacctcag cgaccactcc aagggctatt tcccgacgga aatggcgttc tcgcaaacgt
    91561 tgattgatgt gctggtggac gaaggctacg agtgggccat tgtggccagc caccacttga
    91621 gccgcacgtg cccgacctat ttcgaccagt tcaatctggc tgccgggcaa tacaacattt
    91681 tctcaacgcc gcccaacaag gccgacttgc tcggcccctc gcccacctcc ggctggtggt
    91741 acagcgagcc caatcctggc aacgcggcat ggaacgtggc gccctttgcc tatcaactgc
    91801 acaaggccca gtgggtcaac ccgaacagcg gcgcaaccaa acagatttat gtggtgccct
    91861 ccgacgatgt gctgagctac cgctttggct acgccaacga aggcatcggc aaaatccaga
    91921 gcttcatcgc cccctacgcc accgacccca accgcccggt cattgtgatg cctgcctccg
    91981 acggcgacaa cgcctggggc ggcgggttta gctcgtggca ggaagccacc ccgcagttct
    92041 tcaacgactc tgccaacgcc ggctacgtca agaacacgcc gcaggggttc gtcaatgcgg
    92101 ccaaggcgaa cgcccccgtc acgcacgtgg aagacggtgc ctggattttc ccggaaatgg
    92161 actacggctc gccgaacttc ctcaagtgga ttgagccgcc ggtgaatccg aacagtatcc
    92221 ccacgtgcta ccccaacacc atggtggatt tggaaacgcc gggctttgcg ctcaagttct
    92281 tcagctatgc accgctgatg gcgggcgcca actgggtcat caccgccgag caaatcctca
    92341 aggatgaagg cggcagcgtg caggcgtgga aggtgcagga tccctacaac aacctcggcg
    92401 acggcaactg gaccagcccc aacgatgtcg aattggcctg gcacatttat ctggccggcc
    92461 tggattccgg tttcaattat tatggcggtc tcggcaacga tgatgaagtc aagccggccc
    92521 tcgccaccaa gaatgccatc ggcaagctcc agagcttcat gtccacgcgc ctgca
//
LOCUS       DAOPNP010000002        62802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_603_length_62802_cov_9.148023, whole genome shotgun sequence.
ACCESSION   DAOPNP010000002 DAOPNP010000000
VERSION     DAOPNP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 62802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62802
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_603_length_62802_cov_9.148023"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1636)
                     /gene="glmM"
                     /locus_tag="PKI64_00385"
     CDS             complement(275..1636)
                     /gene="glmM"
                     /locus_tag="PKI64_00385"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD99493.1"
                     /translation="MGENKLFGTDGVRGVANREPMMVETAVNLARAVAYVLKERKGGK
                     RPRVVIGKDTRLSGYMLENALVAGLCSMGGDALLVGTLPTPGIAVLVRDQKADAGFVI
                     SASHNPFQDNGIKLFSADGYKLPDEEETAIENLLAGGKLHKMLPKAGGIGKSFRVEDA
                     LERYTDFCVRTFPADMDLKGLRLVLDCGNGATYKAAPAVFKRLGASVTTLHNTPNGLN
                     INARCGSQHTEALREQVLAERADVGLAFDGDGDRLIVVDETGVELTGDHVLAICAKDL
                     KERGKLRNNLAVGTVMSNFGLHTTMRKLGIELACSAVGDRYVLDLMKERGSVIGAEAS
                     GHMIFLEQHTTGDGIVSGLQLLAVLRRSGQEMSTLASILQLAPQRLVNVDVAQRPPLE
                     TLPTVQAAIQAAEKELGQEGRVFVRYSGTQAMCRVMVEGPTDEMTDRLCHSIATAVKQ
                     AIG"
     gene            complement(1764..2441)
                     /locus_tag="PKI64_00390"
     CDS             complement(1764..2441)
                     /locus_tag="PKI64_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99494.1"
                     /translation="MNELIQARDIRRSFALGARQLDVLRGVSLAVHAGETLAITGLSG
                     AGKSTLLHILGGLDRPTAGEVLYRGRDFYACRERARAAIRAQKIGFVFQAYHLLPELS
                     VLENVLLPGLSLPGAFLQGRKLRQRAGELLARVGLAERLEHRPSELSGGEQQRAALAR
                     ALMNHPELLLADEPTGNLDSQTGEDVLRYLFDLVREQNLTLVIVTHNEAVAARCARHL
                     VLRDGQL"
     gene            complement(2434..3663)
                     /locus_tag="PKI64_00395"
     CDS             complement(2434..3663)
                     /locus_tag="PKI64_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99495.1"
                     /translation="MTLPFSLFLALKYLKPKRTFLSVVTVLATLGVLLGVAVLVIVLS
                     VMTGFDEMWRDKILDFNAHITVSVAGGLGEEEDSLCEQLEAVPGVTAAAPFLQSLMFI
                     QKPNGQVETPLIRGVDPDREAHVSRIPQSITAGEFSLADGAVVIGRDLARRLGVDVGD
                     TITAYSPATFLDQNEIRLPVEMTVAGIFEIGMWEFDMGYVLASLWDARDFCGGTGLEA
                     IQVLTQDPYAADEVAAEIRHRLGVGVHVTTWMEQNQQIFAALRVEKNMMFFLLIFITI
                     VAAFSITNTLITVTVQKTREIGLLRSLGFSAGAVMRVFFWQGWIQGVLGTLLGIGTGL
                     LALHYRNNLLHFLNDRCGMQLLPAELYHLAELPAHTLPGDVALVAVLVLVICTLAAVI
                     PARQAAKLDPVRALRYE"
     gene            complement(3660..5132)
                     /gene="lysS"
                     /locus_tag="PKI64_00400"
     CDS             complement(3660..5132)
                     /gene="lysS"
                     /locus_tag="PKI64_00400"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOD99496.1"
                     /translation="MELSAHEKEFRQQRLANLATLQAAGHTPYGRAFARTGTLAEIRA
                     AFAEGRPVQAAGRVVAVREMGKSTFAHLQDGTDKFQIYLQKNTLGEESFTAFKAIDLG
                     DFIGVSGELFTTRTGEQSLKVAQWELLAKSLHPLPEKWHGLQDTEVRYRQRYLDLIAN
                     PEVRRVFNARSRILSFIRRFLEARGYFEVETPVLQTLAGGAIATPFKTFYNALGQPMY
                     MRIAPELYLKRLLVGGYDKVFELGKNYRNEGLDRSHNPEFTALEVYEAYGDVRTMMKL
                     VEELVSGAAQEVCGTMVVGPADGEKIDLTPPWRVVAYHDLVREHAGADWFERTVESAR
                     DWARARELDIPASMTHAEITHEVYDKLIEKKLMQPTFVTRLPAELVPLAKRCPDNPAL
                     VDVFELVVRGRELCPGYTELNDPLDQRARLEEQAGGDAGKLDEDFLRALEYGMPPAGG
                     MGVGIDRLVMVLTGEESVRDVMLFPQMKTRAPQPDDLPPA"
     gene            complement(5266..6723)
                     /locus_tag="PKI64_00405"
     CDS             complement(5266..6723)
                     /locus_tag="PKI64_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOD99497.1"
                     /translation="MDIRLDQIADHFPAHWREEQAARAKTAFFQALALAAHRFYGGKI
                     QVLPRAPLPGLAWINAWYTPGVSAVSTRIRDHAAESFELSWRGNVVAVVSDSTRVLGD
                     GNVSPPGGLGVMEGKAFLMKILGGVDAVPLCMDSRNARGEHDPEIMIDFVRRLAPSFG
                     AVNLEDISQPNCYRVLDGLRDQCEIPVWHDDAQGTGCVTLAGLLNALKVAGKALADCR
                     IVFLGAGASNTTIASLIMQAGADPARLALFDSRGGLHRGREDIARDPRFYRKWELCQR
                     TNPDRVNEVAVALRQADVLISLSRPGPDVIPLAWVSSMAHKAIVFACANPVPEIYPHA
                     AKTAGALVVATGRGDFPNQVNNSLGFPGILKGALLARARKITDAMAIAAARTIADFAE
                     RQGLTPDYIMPLMTDQAVFAETAAAVARQAAADGVARHAVDPAAIQQRAQEDIAAANR
                     ALELLMSSGLIPAPPADMIQRCLAAALAAGEAPGG"
     gene            complement(6810..7592)
                     /locus_tag="PKI64_00410"
     CDS             complement(6810..7592)
                     /locus_tag="PKI64_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOD99498.1"
                     /translation="MSVRKGLLAAVALGAMVACASAEPMRTVFTMENKFPGALRAEVS
                     LGGGGSSYDPEIRGAEKIKFYDVTAGVRFGLTDRVALVGGVPFVGYSDNRLDEKGLGD
                     VSLGAQFLFFEDIFEYAWIIPHATAIFPTGDEDKGLGTGETQGRFGLSVGTTTMDVLH
                     WAADVSYTMDGAVSDTNPSERDDLTAGSLSLIWDLDERASVLGEVQFRDDPVDPADDY
                     AFRGHAGLAYKVNKHVSVMGYVGGASRMNVDVYGAGRVVVSF"
     gene            complement(7693..9645)
                     /gene="metG"
                     /locus_tag="PKI64_00415"
     CDS             complement(7693..9645)
                     /gene="metG"
                     /locus_tag="PKI64_00415"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOD99499.1"
                     /translation="MKPDSYYITTPIYYVNDRPHIGHAYTTVLADVLARSQRLFGRPT
                     WFLTGTDEHGQKVQEAAAKLGVDPQVHADNTVVRFREAWQRLEIQNDDFIRTTEPRHK
                     IIVQEILQDLYDRGEIYRDEYDGWYCVPCERFFTARDLVNNNCPECGRAVQRVREANY
                     FFKMSQYQDWLVDYIQTHPEFIQPDFRRNETLGFLRKPLQDLCISRPKSRLSWGIELP
                     FDKEYVTYVWFDALVNYISAIGYRRDDATFARWWPAIHLVGKDILTTHTVYWPCMLHA
                     LGVPLPKTVFAHGWWLMGSAKMSKTLGNVVNPMELMDRYGVDAFRYFLMAEMVLGQDA
                     SFTEEGFIRRYNADLANDLGNLLNRVVSMIGKYCGGRLPAPETGAFQKGPAAELFRQV
                     AAATASFRTATENFTLNAGIAQVIEAVRATNRFIETQQPWTQARAADPGPLRTTLYAA
                     AEALRICAGLLMPVMPAKMTELRAALGMENPMTVDPKELSTPGILPPDVAVAPPGALF
                     PRIEAPVPDGERPATPPAAPAAPKAAPPGTIGNPEGIITYDDFAKVQLRTAKIMGAEP
                     IEGAVKLLKLNVLMGDERRQIVAGIALYYKPEELIGKTVIMVANLAPIKLRGVESQGM
                     LLAASKGERLRLVTVDGDLSSGAVVK"
     gene            complement(9703..10647)
                     /locus_tag="PKI64_00420"
     CDS             complement(9703..10647)
                     /locus_tag="PKI64_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD99500.1"
                     /translation="MAEAIVSHAPRFLVLRGGAIGDFIATLPVLQALRARWPAAHIEI
                     WGYPHIAELALAVGLAQSIVSLDRADMARFFVPKPDLTPTQVAAVRSFDLVFNYLHDP
                     VGQVRSNLLLAGAKQVLSGSPIIKEGHAIPFLLAPLEALAIYDGDPHPVLDFPAELRA
                     QGAARLRDRGIRGRPLVVHPGSGNPDKNWPAARYVEIITRWRAQGREVVAVIGEADLD
                     EAAALQRNLPDLPFLSGLTLPELGATLAECAAFLGNDSGITHLAAAVGLPVTALFGPS
                     NPATWAPRGRGGVRILQAPEGELARLTVNAVWMALGES"
     gene            complement(10658..11212)
                     /locus_tag="PKI64_00425"
     CDS             complement(10658..11212)
                     /locus_tag="PKI64_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99501.1"
                     /translation="MAPARYLGDLLILLGLLSVLVGAVLWRCPGASRRMLQAFPRAKW
                     AGWALTAVDVFWVTWVVRHAALGWFEPFKPLVPVLGVVVFVLIVWLLDELLAPRALGG
                     LLLLLANPMLNSVRWHDSAWRLVVVTIAYAWVVAGALLVLYPWFFRRWAAKPLAHPQG
                     LRVLAVAKLVAGVVLLIAGVAHLR"
     gene            complement(11187..12164)
                     /locus_tag="PKI64_00430"
     CDS             complement(11187..12164)
                     /locus_tag="PKI64_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99502.1"
                     /translation="MKRSMAWGLALVGALLWAAPGRAQVELSWSLAHNRTLLMEPVLA
                     TVRVVNNSGVTLDFTPRGNARLSFDVEDQPTSPVPTTGRPLLPRPILIPAGETRDVDV
                     NLLEAYVLIKGQTYKVTPVLEFAGTRFIGERQYLEVQPGLELLKRTYGVPNTPSAREV
                     SLRQLNRDRNDRLFFRLDNPQAGICLGAYELGRVIRFFVPTLELDHNGVFHTLHQAAP
                     DRFVHSQFSYDGALLGATLYVGTAGAMKLARTDTGDLEVQGGTPFTTDPDNPEILVAP
                     ALPPAHPFPKSLEDVRARRKAADKKAPPRKKTRPAPSRGQDDGTSPVSW"
     gene            complement(12173..12904)
                     /locus_tag="PKI64_00435"
     CDS             complement(12173..12904)
                     /locus_tag="PKI64_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOD99503.1"
                     /translation="MNLKYAILGDVHANWEALSAVLADAEKQGVTHYCCVGDVVGYNA
                     DPELCLAKVRELCGDNVVRGNHDHYCSRQENLNGFHPLAADVVDWTRKRLDDEQRAWL
                     GNLRYSRTVETFMLVHATLDNPDMWGYVFDKLEAEANFAYQMCSVCFFGHTHVPLAFE
                     KAGIVRGGLYTKLLITGGRKYYINVGSVGQPRDGDPRAAYAIYDLVQNVVELRRVEYD
                     IAGAQKKIRDAGLPPRAASRLEIGR"
     assembly_gap    13051..13150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13151..14713)
                     /locus_tag="PKI64_00440"
     CDS             complement(<13151..14713)
                     /locus_tag="PKI64_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99504.1"
                     /translation="MNMDEEASPEKQAQDNKPDLALDLNFVPTWARTPPENPYARHED
                     GGEYERDRERDRRPGGRDRRPGSFRRPEGGERRGPRRDGGSWGGGRREDRPRPGGRSG
                     PPREGGRPPRGAEQDRRGPRRDGERRRDDRRPPREALPRLNVKVSFLPDRERLALVVR
                     DIQASRRAYPLIEIAQRFLSREDSYLVKLEMPPAEAGQPRPVLVQCLECKRVFRHRAN
                     AEAHVLHDHLDKFFTIEEVDCEPPAGNFTFVARCGLSGTLLGPPNHHGYNEQIQELWS
                     RRFAHMSKAEYLGHIETVRDEALVEQWKDSLRKKTTYRLTTVPEGQEAETWTRAQAQE
                     WMSAGKVNGLLRESGRCLLPAHQSKKFDDGSLRQAVSLAWQRESRFPFTLSLALRPAF
                     RHMRLHLFKVNARETYVTAVLPKPVDPNTAPALVKEIVDYIEQHPGVTRQQVLEGLRP
                     GRNPEEADAAELLLHLSNQVATGGVIEFFNATLALPRGGAEAKPTATGGKAEPAAPTV
                     TETGDPAELAAEP"
     gene            complement(14924..16561)
                     /gene="groL"
                     /locus_tag="PKI64_00445"
     CDS             complement(14924..16561)
                     /gene="groL"
                     /locus_tag="PKI64_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOD99505.1"
                     /translation="MSAKQIVYDAEARQLVLKGIEKLSKAVKSTLGPRGRTVVLEKKF
                     GSPTITKDGVSVAKEIELENPFENMGAQMVREVASKTSDVAGDGTTTATALVEAIYRE
                     GLKNVTAGASPMGIRQGIDRATDAVVAALQKQSKKVKDNEEIAQVGTISANGEMAIGK
                     IIAQAMEKVGKDGTITVEEAKGIETTLDVVEGMQFDKGYLSPYFVTKADTMEVMLEDA
                     YILLFEKKISNLQELLPVLQNVAKSGKPLLIIAEDIEGEALATLVVNKLRGTLQVCAV
                     KSPGFGDRRKAMLEDIAVLTGGKFISEDLGIKLESLSLADMGRAKRISIDKDNTTIVE
                     GAGKTSDIQGRIAQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAQAALDDLKVSGDAKIGVDIVRRACETPL
                     RQLVENAGLDASIVVQDVRNGKGAYGYDVATGQYVDMIKAGIIDPTKVTRSALQNAAS
                     IAGLLLVTEAMVAEIPQKKEATPPMPGGGMGGMGDMY"
     gene            complement(16588..16875)
                     /gene="groES"
                     /locus_tag="PKI64_00450"
     CDS             complement(16588..16875)
                     /gene="groES"
                     /locus_tag="PKI64_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOD99506.1"
                     /translation="MKIQPLGDRVLVEPVKEEEVKKGGIIIPDTAKEKPQQGIVIAVG
                     TGKVDDTGKKIPFNVKKGDKVLMPKYGGTELKLNGKEYQIMREEDILGILE"
     gene            complement(16953..18887)
                     /gene="dnaK"
                     /locus_tag="PKI64_00455"
     CDS             complement(16953..18887)
                     /gene="dnaK"
                     /locus_tag="PKI64_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOD99507.1"
                     /translation="MSKVLGIDLGTTNSCMAVMEGGQPTVVTNAEGGRTTPSVVAFTK
                     TGERLVGSAAKRQAVTNPKNTVFSIKRFMGRKYDEVKEEAKRVPYEVVRAANGDAHVK
                     IGDKTYSPPEISSMILQKMKLDAEAFLGETITQAVITVPAYFNDSQRQATKDAGRIAG
                     LEVLRIINEPTASSLAYGLDKKSDEKIAVYDLGGGTFDISVLEIGDGVFEVKATNGDT
                     HLGGDDFDQRVIDWLVAEFKKDNGIDLSKDAMALQRLKEAAEKAKCELSSAQSTDINL
                     PFITADASGPKHLNVALTRAKLEQLVGDLIDRSMTPVENCLRDAELPKDKLDEVILVG
                     GMTRMPRVQQVVEKQLGKEPHKGVNPDEVVAVGAAIQGGVLKGEVKDVLLLDVTPLTL
                     GIETLGGVMTPLIERNTTIPTKKSEIFSTAADNQPTVEIHVLQGERKMAADNKTIGKF
                     HLDGIPPAPRGMPQIEVAFDLDANGILKVSAKDLGTGKEQKITITASSGLSKEEVDRM
                     VRDAEAHAGEDAQRREAVDTKNRAEAMVYETEKFLKDNGEKIPSDKKLKVENGIQALK
                     TAIQSGDTDAIKKASEELTNDMQAVSADLYAQAKQQAPDGNAAGGSAPPPPHEQAKAD
                     GDVMDADFEMVDDDQKKNSK"
     gene            complement(19465..20409)
                     /locus_tag="PKI64_00460"
     CDS             complement(19465..20409)
                     /locus_tag="PKI64_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99508.1"
                     /translation="MDLVAIIRSKIERAGEGNHFLGLKAVLRHIVTAVAHFDRGQDAA
                     DETAFTDCIYRTNQAFEGSLKEAFRVLTGKDPDKKTPHNIEEHFASNDVFRSRVLRQF
                     KNYRTEWRNPSTHDHKLDFDASEAFLAIVSVCAFTNVLLDQIIEKLAHDRSKLESKKE
                     RMRIAKALKAKGTDLASFAANLISEFISQYKPAHEEPRMREAEFLGSLSGFIESIAPK
                     LNVQIEVLLGTDRHYRGDLLLSRGNEKLLVELKGTSFSRQLVRSGLEQLDRYFSISGI
                     DQGVLVIANPPYATPTIIDATTPRGRRVLVVAPRQTET"
     gene            complement(20700..21659)
                     /locus_tag="PKI64_00465"
     CDS             complement(20700..21659)
                     /locus_tag="PKI64_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HOD99509.1"
                     /translation="MRASKKSKYIKAANQILVSLGLPKGQQNERSALSLLALLNLTPG
                     KSWAGAENPLMGITPIMNWAREHYGKEYAPNTRETFRRQTMHQFCDAGIALYNPDRPD
                     RPVNSPKAVYQIEAAALALLRTFGTPAWHDRLTAYLAERETLVARYAKKREQNRIPVK
                     FAPGKKITLSPGEHSELIRAVIEDFAPRFAPGSVLVYAGDTGDKWGYFDASLLAGLGV
                     NVDSHGKMPDVVLHFITKNWLLLVESVTSHGPVDGKRHAELAKLFAGSTAGLVYVTAF
                     PNRAIMGRYLGEIAWETEVWVADAPSHLIHFNGARFLGPYHAP"
     gene            complement(21656..23110)
                     /locus_tag="PKI64_00470"
     CDS             complement(21656..23110)
                     /locus_tag="PKI64_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOD99510.1"
                     /translation="MRLSKNTDAKKKSQYGQFLTPGGTAAFMAGLFPSADGTCRLLDA
                     GAGIGSLAAAFLERWSFGGFGFQRVEVDAFEIDDSLHSELMRTLYKFASSRLSVAVHG
                     DDFIHTATDALCGGLFSKLLPRYTHVILNPPYKKINSNSAHRLALRSIGIETVNLYSA
                     FIALAVALAAPGGQIVAIIPRSFCNGPYYRPFRDFILERAAIRHMHLFESRSAAFKDD
                     AVLQENIIIRLERGGQQGSVMVSTSTDDTFADLETHEHPFGRIVFPDDPERFIHVPTT
                     PEKHAIELTETICYTLADLCINVSTGPVVDFRLKEHLRDMPGEGSVPLLYPGHLSSTG
                     TTWPIEGMKKPNAIERNADTEKWLYPSGFYCVVRRFSSKEEKRRIVANVVKPSAFGDV
                     PVLGFENHLNLFHENKRGLPEALAHGLAVYLNTTAADEIFRRFNGHTQVNATDLKAMK
                     YPSREILIKLGEWAMQQGELTQSMIDEKFWGIAI"
     gene            complement(23484..23981)
                     /locus_tag="PKI64_00475"
     CDS             complement(23484..23981)
                     /locus_tag="PKI64_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99511.1"
                     /translation="MYILTHQVGSFPVGIWAVLAMLFVGGGVFTIFGQGLSLFNWDAA
                     LRLRLQEDSRRSQDPLERTMGAISQGEAVADVAVQGVLILLSFVGILLRNPVGFVAGI
                     AQGVIWIYATFLVLAQRWMLYRWGIVNNLARLKHVAPAMLLAGGIPGALMIICLVANI
                     GFFGW"
     gene            complement(24075..26534)
                     /locus_tag="PKI64_00480"
     CDS             complement(24075..26534)
                     /locus_tag="PKI64_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99512.1"
                     /translation="MIDKAIQRFRKLVESLEVRDISAANEATTRKRVIDTILESVLGW
                     NPDADISYEERVTEDNNTRYSDYIVRTPATAFILEAKREGTTFNIPPNRRTCLLGGAV
                     AGTAAGKACAQVRDYCRKKNIPFAVATNGSCWIVFPAVRTDGVPYEKTQAHVFQDFAD
                     IDARFIDFWNLLSRERVIEGGLETTLFGSTDDMVRRRLLTVVKEPGFRIGRNAVYEHI
                     EPTVAIAMTDEALLRDVDGLRFCYVKTSERLKYDSRLRTYFMDVKPDLERRVIRPRRK
                     KSKQTHLDSLIQTTHIERPLQFILLLGPVGAGKSTFLEYTRQVTAASIIDNKILWMKV
                     DFKHATNADDPRQFIYGRLLDAIDSDREFSLASWEKTQSHAYETMIATLKQGALALLA
                     ECDEPAFKKEIASKVYDDRKQVIPFVRTVMRHVTSHTPSFLVVDNVDQIEDVEYQKRV
                     FLETQAAAREMGMHAILSLRDSTYLRHMTTPVFDAFQMDTLFIDPPQVVPVLSRRFAY
                     AKKFITGKKATIVGESGYRFVVDDLGSFFDILTDSILSEETGFMLEVLSGNNVRRGLA
                     LVRNFMASGHTSADKALWEFARTGSFKFAPHEFYKGAIFAQRKYWREEESTIPNIFDS
                     HYGRVPTQLLRYLLVSRLKRLGSKEDFSGESARAIIEFLHSCGVSRKDGATLVQDLLG
                     YGLINTRDGRPMDDDATILATRLGAYLVQELSSRFYYFESCLIDACVHDDVFWDGLVE
                     ATQKIEASRGYARMEARVQRASLFLKYIRQCQEEWIAHAKKYGADDLWTDNYVQALEH
                     ALKSEFARVLASAQRQYQRLR"
     gene            complement(27084..27455)
                     /locus_tag="PKI64_00485"
     CDS             complement(27084..27455)
                     /locus_tag="PKI64_00485"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD99513.1"
                     /translation="MIPEKLLEIMKHEGVVAIATMGKDGPHMVNTWNSYLRISSDGRL
                     LVPAGRMQQTEANVAGNPDVLMTLGSREVPGRHGPGTGFLIKGKAAVITSGPDFDFMK
                     TQFSWQRATLAITVESATQTL"
     gene            complement(27559..27942)
                     /locus_tag="PKI64_00490"
     CDS             complement(27559..27942)
                     /locus_tag="PKI64_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOD99514.1"
                     /translation="MFKRIDHVEIIPRNAERTIRFYTDILGFQVISREAVNAPPLKEV
                     IYLRLGDTTIEVMSVDDPKPGAESPWQIGLRAIALEVEDMAKAVEYLKTRGISMSLAP
                     VDLGNSYRGELRDPDGLIIELRQWK"
     gene            complement(28246..28707)
                     /locus_tag="PKI64_00495"
     CDS             complement(28246..28707)
                     /locus_tag="PKI64_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOD99515.1"
                     /translation="MRVLVDTSVWSLALRREAQVRHPEAEELKNLIAARAVEIIGPIR
                     QELLSGVRDQTQFVRLESSLAAFPDLSLVTADYVLAAKFFNACRAKGIQGSNTDFLIC
                     AVAVRSDSAIFTTDLDFGQYKKCLPIKLYEFKSHAEQSPARYRRSVPKRNH"
     gene            complement(28704..28904)
                     /locus_tag="PKI64_00500"
     CDS             complement(28704..28904)
                     /locus_tag="PKI64_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOD99516.1"
                     /translation="MATNLQLDDRLIREAVSLGKHRTKKAAVTQALTDYIQHLRQKRI
                     LNVFGTIEFDPDYDYKKQRARP"
     gene            complement(29254..30513)
                     /gene="hisS"
                     /locus_tag="PKI64_00505"
     CDS             complement(29254..30513)
                     /gene="hisS"
                     /locus_tag="PKI64_00505"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOD99517.1"
                     /translation="MSSTTQPVQGMADIAAPEVRLWQRLEELGRAVLARYGFEELRTP
                     IVEFEAVFTRSLGATTDVVQKEMYVFEKGGRRLCLRPEGTAGVMRHAAGLGEQETAGA
                     RWFYLGPMFRSERPQAGRRRQFHQLGVECLEPPSPLVDAEILALQVDLLRAWGIGDCR
                     VRLNTRGEAGGHAKVAAGLRAALAAHEAELCDDCRRRIGENVLRVLDCKNAACQRIVA
                     QVPPPTAFMSEASREYLAQVAQHLAALDVAHDLDPLLVRGLDYYEHTVWEIASTALGA
                     QNAVAGGGRYRLQLGGRELTGVGFAIGLERVVGVLQALGRDAALLTAPPPLVWLVGLG
                     DAAMQQNLGLMQQLRAAGLVCRMAPAGSLKSQMRAANKAGAAWAVVRGDDELAAGVAA
                     VKNMTSGEQQNVALAQLAAALSAPPPA"
     gene            complement(30580..30876)
                     /locus_tag="PKI64_00510"
     CDS             complement(30580..30876)
                     /locus_tag="PKI64_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD99518.1"
                     /translation="MANEKQNLTKRNLAIRIAEETGLTQQQVQAVIQKTLDSLLDALR
                     EGQTVEFRRFGVFDVRERRQRIGRNPHRPDQVVVIPSRKVVRFRPGKDMKALFK"
     gene            complement(31396..32511)
                     /locus_tag="PKI64_00515"
     CDS             complement(31396..32511)
                     /locus_tag="PKI64_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOD99519.1"
                     /translation="MKVCMDIQAALGQRAGVGRYVRELLWHLGPLAGTDEVSAFFFDF
                     KRQGLPELPPAVALRACRWLPGRVAQNFWKRLGWPPYEWLAGAADVYHFPNFIRPPLG
                     RGKKSVVTIHDVSFLRMPETTEARNLAWLSAEIHRTAERADAILTDSHFSAREIVELL
                     RVPAEKVFPIWLGLPKFGPPLAPEAAEAAREAIGIRRPYLLMVGTIEPRKNIPFLIKI
                     FEALTDFDGELVVAGGLGWKTGPTLSAMTYSPRREQIKLVKHMNDAQLSALYAGAAAF
                     VYPTRYEGFGFPPLEAMSRGTPVIAARNSSLPEVLGDAPVWVDGFDVEEWAAAVRGVL
                     AEPARADRLRAAGLAQAAKFTWEETARQTWAVYRQLA"
     gene            32638..33648
                     /locus_tag="PKI64_00520"
     CDS             32638..33648
                     /locus_tag="PKI64_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99520.1"
                     /translation="MSEFKLTPEEPPPPERKKIRLTKAEAYALSMASPKKKRIAFALV
                     GLLIAAALGNVLWYFQDHWLPFWFPEKPAAAPAVEPAPTAAPAISDTAPTAESVAPIT
                     DPTALDFLSATVWDDPQFLQGVRLFNQTLDRYRTETSSPLPPVGLEQIEHNALQAAAQ
                     FSALREQAPATVPLADYIARCQQLALAARRARNPEATATLPPPPPPPPARPTAPPEPA
                     MPLKPGETWQDPEYLLGANLFNRALAQYRAFLNNKAQTHLLKEIEDNAFQAAKTFEAI
                     KPRAPEGVPIADHIAQCYRLISDCRRQNLERGADAPATGGPQGNVVGPRHRPALPAYQ
                     AP"
     gene            complement(33679..34908)
                     /locus_tag="PKI64_00525"
     CDS             complement(33679..34908)
                     /locus_tag="PKI64_00525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOD99521.1"
                     /translation="MNVVAVILGGGEGKRLQPLTRDRCKPAVPLAGKYRLVDIPVSNC
                     INSGFRRIYVLTQFNSVSLHQHVQGTYVFDQFHSGFVRILAAQQTPSSESWFQGTADA
                     VRQGIRYIMDREPDYILILSGDQLYQMDFRPVMEAHIRNKAEVTICTKPVPRAEAGAL
                     GIMQADEKDRIVRFVEKPGDTPLLDELREPGNGPERYLASMGIYVFNTRVLLELLANE
                     EKDFGKNIIPAAIKDRAVYRYLFEGYWRDIGTIRAFWEANMELAEMVPQFNLYDVNAP
                     VYTHMRYLPPSKINACDLNRALLSEGCIITGHRILRSIIGVRAKVGTGSVLEHTVLMG
                     ADWYDPEPLPPGQIPMGVGRDCFIRNAIIDKNACIGDGCYITPDGKDNVVTDQYTVQD
                     GVIVIPKKAVIPAGTHI"
     gene            complement(35108..35866)
                     /locus_tag="PKI64_00530"
     CDS             complement(35108..35866)
                     /locus_tag="PKI64_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99522.1"
                     /translation="MKIWLGGARGSFAVSGPEYGRHGGDTFAMLVEGQDGAQLLLDAG
                     SGVRRLLSRLRADYVLYFTHTHLDHLLGLPLLENKWPRQLVLPRGDLAATLARVFSPP
                     IWPVTLSTADYSVPAMQAAVGGLVIRWHEVAHPDGCVAYRVDEPATGESMVVATDCEW
                     PAMAPAAQESFVKFAHGADLLVFDAQYLPEEYPAHRGWGHSTWADAVAVAQRCAAKRL
                     WLIHHKPKRTDAELAALGAAAAAVFPGATVPVPD"
     gene            complement(35874..38006)
                     /locus_tag="PKI64_00535"
     CDS             complement(35874..38006)
                     /locus_tag="PKI64_00535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD99523.1"
                     /translation="MNKKLRYGLLAGVLATAVAGLLQWGGLLARWENPLADWRARLLA
                     KPSAATDDIALILVDQASLDWASQNIGLSWPWPREIYAPVLDYLTRNQARAVAFDVLY
                     TEPSTYGVEDDEALGAAAGRAGNFVAAVFLGTTTTQTDAWPEIAQPNPLHLAGWADWL
                     AAKPAARADMVSAAAAFPVPELTTNTAWLANVREVPDSDGIFRRAVPLRFFADQVLPS
                     LGVACWLAGQPTPVAGQLTGNTLTLAGRQIPLDDSGRMILKFRGPSGTHPAYSAAAVI
                     QSELRLQEGGTPPIADGSVFKDKFVVFGFSAPGLKDLRPTPVAADYPGPEIHATMLDN
                     LLIGDFLRDVPPPAAWGWTLLLALLAGLGAAAARNGWQSAVLAVALILVPVLAGLAAW
                     QANWVLPVAGALTSVILALLGGAVVNFILEGRQKMFIKGAFKHYLSAEVIEQIMRDPN
                     SLKLGGERRELTIFFSDLQGFSAISERLSPEELTRLLNDYLSAMTEIILAEGGTLDKY
                     VGDAIVAFWNAPLAQPDHAVRAVRAALRCQRVLAEQQAAYAQRIGGPLKMRIGLNTGA
                     VVVGNMGSSQRFDYTMLGDAANLASRLEGANKAFGTNLMIAESTWEQTQGAFLGRELA
                     QLTVVGRKTPVRVFEATGFAGEPPAPETHAFADALALVRAGNWVAAAAGFAALSADPA
                     AQVYTAKCRELANHPAATWDGIWNLAEK"
     gene            complement(38025..38945)
                     /locus_tag="PKI64_00540"
     CDS             complement(38025..38945)
                     /locus_tag="PKI64_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD99524.1"
                     /translation="MKSAFCKKLGSALVVGSLVLAAGCETVNQITAAAADVAAATGAI
                     TPEQAESTKRVSTAVTKTFQDITPEQEYYIGRTVAATVLTQHKPLPAEKLNAYLNQVG
                     QSLAQFSERPETFGGYHFLALDSAEINAFAAPGGLILVTRGLLDCCQTEDELAAVLAH
                     EIGHVEKQHGLRAIRTGRLNSALTILAVEAGKNLGGEQLAEVTKAFDESINDITTTLM
                     NSGYSRKLEYEADAAAVGILRRAGYQSGALVAMLENMAKNWDSSRHDFAATHPAPADR
                     IAQLQKLGVASTKASAPARQQRFLAATQGQ"
     gene            complement(38956..39417)
                     /locus_tag="PKI64_00545"
     CDS             complement(38956..39417)
                     /locus_tag="PKI64_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOD99525.1"
                     /translation="MKKWWLMASLLLVGASAWAAKEMSVQVRDGQLRNRASFLGAVVA
                     NVAYGDRVTVCAEQAGWCEVTTAAGARGWIHSSALTPKRVVLASGASDAQVGASGAEV
                     ALAGKGFSKEVEAEYKKQNRELDYTWVDWMGQQTLTAEQLVQFLKQGGLAQ"
     gene            complement(39434..40330)
                     /locus_tag="PKI64_00550"
     CDS             complement(39434..40330)
                     /locus_tag="PKI64_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99526.1"
                     /translation="MAVLAVMLLAGCATPPAPQVTDEDWVSHVTTGRGLYARGDYRRG
                     ASAFERAQVRARAMDDANALAVAAVNRAQCLLAEDQPGEARTAIEEALADPRVSRERR
                     MELLAAGARADWGVGDAPAALARLAELQAMTPPALLRAQAALLHSEIQLAQGDAAAAA
                     KALADGPKPNEWQRVPASLRAARAGQQASIAAVGGDPAAAVTHWDEAARLWQQADRLP
                     EMARALAAGAQQAQAAGDAAGAGERLYRAARSLWAQGVQPEAVARLQEGLAIAEQMPD
                     SPLAKKLAALYVTFQAEQRLNK"
     gene            complement(40426..41349)
                     /locus_tag="PKI64_00555"
     CDS             complement(40426..41349)
                     /locus_tag="PKI64_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD99527.1"
                     /translation="MAKPFKFRYVNEIVGGFVLLVVLLLLAGVLVAGHAQHWFEAKYM
                     INLNFPAEGSMDLQKGAEVMLLGAKVGSVQDIVVNDDGHISGVMRIRGPFMRFVRADS
                     IALVKKKMVVTGDAYIELTRGTGAELPRTGGEIECVKDTEILEMLQEALETIRKEAVN
                     TLTLVNGAIAEYTDLAAGMNDPDGSLQQLLANSNGLLDDLRKGPGLPAKILNDPGMAK
                     DVENIVAQIQELMDKINAIAAELQTMVSTIAPVSGTVADEVDNLPVLTGDAQTLMRET
                     TALLDGLQKHWLLRKYMGSDDALRAQEVLVP"
     gene            complement(41359..42006)
                     /locus_tag="PKI64_00560"
     CDS             complement(41359..42006)
                     /locus_tag="PKI64_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99528.1"
                     /translation="MTGNPNSELWRVAGVRARQGPVWNFSVAAGAAAWIPDSDDDGLD
                     WLDWLLGLTTPPEGAVHWQGRDWQAYSPDAASAARGTIGCVLTTGGALANLDMDENVW
                     LPARIHRRTGAAAEIETWARRFRCWPLPARRPAVVRERERQRLLWTRAFAGRPLALVL
                     ERPLRNMPEEDRRLFLAAVADVRAAGCAVIWLEETLAEDVRAALAPLTVITPEPA"
     gene            complement(42014..42802)
                     /locus_tag="PKI64_00565"
     CDS             complement(42014..42802)
                     /locus_tag="PKI64_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99529.1"
                     /translation="MSFLGYIGSGVLTRWSRLTRLAAITWAVLLRAIRPRTWTAPLRN
                     VLARQVLFTGFEATGFIALIAVIVGALVVVQAQYWLTRLGQSNLIGPILTAVVLRELG
                     PLLTNFVVIARSGTAIATELANMKVHGEVRALDAMGIDPFVYLVVPRVLGVAISTFCL
                     TIIFLAVTFIGGFACIWAIELGDVNMGLFFGNIIGSVTVPDVFSLLAKSIVPGLLTGA
                     ICCDEGLAVGVAATEVPIAATRGVIRSVATLFVTSLLISIMAFV"
     gene            complement(43190..43819)
                     /locus_tag="PKI64_00570"
     CDS             complement(43190..43819)
                     /locus_tag="PKI64_00570"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD99530.1"
                     /translation="MAANTRSVGILPATPANIRHAAAALRAGELVGLPTETVYGVAAR
                     ADDPAAIARLFRAKGRDESKQVARFVADLDGVRTAGATVSDIARQLAVAFWPGPLTLV
                     LPGADGAWLGFRAPAHDVAQAWLRELGGVIPAVTSANRSGKAPAGTAAAAWAALSPHL
                     SMVLDGGPVTGGQPSTVLRVAGAEAEILRPGPITAEHLARIPGLHLRST"
     gene            complement(43803..45086)
                     /locus_tag="PKI64_00575"
     CDS             complement(43803..45086)
                     /locus_tag="PKI64_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD99531.1"
                     /translation="MNRLWNQGRALCLAGLWLAVHASAQSDEPAAVEPAADAAGLCAT
                     IAEGERLLAENHLPAPDDAAKGALLEALLRASEPTVVFLDEEELASRCQQEATQEWTL
                     GLTLVEMEDSLPKIVAVAADTPAATAGIQPGEFVQKIGDQDLATGATLKQVRSLLLAG
                     EEPELAVSILDPTGQSRALTLVRTRRERTALTNIEDLPVNMGYMRAAGIFTGAGAEIV
                     ACLDKWQAAGKIGAILDLREADGTAEDEVAIVAAKFTPRDDVLYTMNDRQGHEIKTVK
                     ATAPAAPTLPLMVLVDGETCGAAELLAAVLSGSVQGAMVIGQETAGDPLIREAKQLPT
                     GRYALLATRQLKAANGAVYAGGRGVQPDVVISSAALHETFYEPESPVLRKGKTLSEEE
                     KEDRALRDRTRHDAYLRRATDVLLGLKALGYGRQH"
     gene            complement(45108..46073)
                     /locus_tag="PKI64_00580"
     CDS             complement(45108..46073)
                     /locus_tag="PKI64_00580"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOD99532.1"
                     /translation="MKRQLVALLVLGSFASSAWAFQAGAIAGSRDGAGVPVAFQASLG
                     VLNGEAKEHVYDYEGPGGGRRQLSRLDWDLKGIAMGGVNGSVRLMDALTINGGVWLAL
                     SKGSGEMEDYDWLIVDSADWTHYSLSDVDIESGYIFDLNVAWDFQLTQDLVARAMVGY
                     KQNGWEWKDRGQYLLYPEYGYVPQDLGGENMINYEQEFRMPYLGASADWKTGNFTLSG
                     YLTWSPIVAADDWDVHVARDVHYHETFDGGNMLGLGLSARYDFTQGSLQGVFLTAALD
                     YQKISRIVGDMELTDGSTGESGGEEDVAGIENSYVIFSVGGGIRF"
     gene            46364..48478
                     /locus_tag="PKI64_00585"
     CDS             46364..48478
                     /locus_tag="PKI64_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD99533.1"
                     /translation="MPQLEHIEAIEKRLWTAADTLRANSNYASNEYFMPVMGLVFLRY
                     AYSRFLAIKDGIEAGLPTRGGKKRDLTKEDFSQKSAIFLRPEAQFDYLVSLRDEEDRA
                     KAIIGAMKSIETDYVSLKGVLPKEDYQELDNAVLGQLLRILNPEELKRVSGDVFGRIY
                     EYFLTQFAGQKAHDDGEFFTPVSLVSFIAHVVDPKRGIVLDPACGSGGMFVQSARTVE
                     EHGQSPAERLTFYGLEKNATTIRLAKMNLAVHGLEGNIQKAITYYEDPHHLLGKADAV
                     MANPPFNVDEIDAGKMKSDPRLPFGLPGTNKRGKVSNGNYVWISFFYSYLNRRGRAGF
                     VMSSQASSAGNEEAKVRQKLVETGDVDVMVAIRSNFFYTRTVPCELWFLDRDKPAEHR
                     DKVLMIDARNVFRKVTRKIYDFSPEQEKNLLSIVWLYRGETDKYLALLAEYCRRTLEE
                     AEACFAPPTDGGQDAEPLPEFIAAMDQLSAAMKPFLNTPAAEAALAAQISELDAARQE
                     LPSAANDFRKATANHRAAWKKPPATNAELKKVVERLAELADSSRDLVHQADLCYKLAG
                     RLIASCEKECKAKENEKWVRRDINHASKAAKEARETAVAQLKQVRYFWKQAHWLTERF
                     PDAKLRDVAGLVKLVDRAEIAANDWSLTPGRYVGVAPEEADADFDFEETIQDIHLELA
                     GLDAEAVQLAAVIQRNFLELGI"
     gene            48475..49665
                     /locus_tag="PKI64_00590"
     CDS             48475..49665
                     /locus_tag="PKI64_00590"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOD99534.1"
                     /translation="MSWRPQKLDQLGTLSRGRSRHRPRDAAHLYGGPYPFIQTSDVKH
                     SGLYIREYQQTYSEEGLRQSKIWPAGTLCITIAANIADTAILGIDACFPDSVIGFTAD
                     ENKSDVRFIKYLFDATIQQRVRQFTQGAAQDNLSQEKLLSIEFLIPELQEQRRIASIL
                     SAYDDLIANNRRRIQLLEEAARLLYREWFVHLRFPGHEHAKIKDGIPEGWKRGPLGQL
                     CVEVREICQPASLEPDAPYIGLEHMPRRSIALSEWGTTEQVTSSKHRFRENDILFGKI
                     RPYFHKVGIALTEGIASSDAIVIRPASQELLPLVLMVTSSDAFVADTAQKMKEGSKMP
                     RADWKQMTQYPVLYPSDNTLWALNDFIEPILQQIKTLVFSNRNLAVARDLLLPKLMSG
                     EVAV"
     gene            49665..53447
                     /locus_tag="PKI64_00595"
     CDS             49665..53447
                     /locus_tag="PKI64_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOD99535.1"
                     /translation="MPAFATNGADIPERLLQAHEDGRVVFFCGAGISYPAGLPGFSGL
                     VKDLFSHLGQEPTAVQQAAIKEKRYDTAVGLLETEYVGGRERVREAIKKILTVTEIKT
                     TAVATHEALLRLATNSEGKIRLVTTNFDRLFEHVIQHSGLSVETFAAPLLPIPKNTWN
                     GLVYLHGLLPDSNGKPDLNRLVVSSGDFGLAYLVERWASRFVSTLFRNFTVCFVGYSI
                     NDPVLRYMTDALAADRQLGEHPPEMFAFGSYPKGKQIQAEEEWRAKNVTPILYREYSK
                     HAYLHRTLKEWAKTYRDGVRGKEMIVVQHAATPPLSPSPSDYVAGRMLWALTDELAAR
                     RFANLDPVPPLGWLDLLTNEQFSHKDLPRFGVAPNDKPDEKLSFSYLRRPTPYVKAPW
                     MCLADMGMPSPSMDAAMREMAIWLSRHLNDPKVLLWLAKQGGRPSRRFVDIIHDRIEE
                     LDRLIAKNDTARIAEIRAAAPNAFPSPAMRALWRVFLAGRVGYGIDRLELHDWFDRLK
                     INGITPSLRMELREMLEPKVMLKPPFRLGLEEEEEKEEPTVRDLIDWELTLATGHVHS
                     LIQHLGHDNPEWKRALPELLDDFTMLLRDGMDLARELGGAEDAHDISYIYQPSIEDNG
                     QNHYFQNWPVLIKLTRDAWVETAKTDTTRAVQVAMAWWRLPYPVFKRLAMYAATQGNV
                     ISPKLALEWLLDGERQWLWSIETRRETIRLLVALSPQLGESDLAALTAVILNGPPRSM
                     YRDDLSSERWQEILEHDIWLRLAKIRKAGGILGHDAQTKLDELEGKYPEWEVTDDDRD
                     EMPFWISDGKDLRSFTPTPRRRIELIGWLKDNPDTDHWKENDWRQRCDTDYSAAIYGL
                     YALSKDDIWPEGRWREALQVWAEDNQTTRSWAHIHCLLEQMPDSVARKLDRSICWWLR
                     AVAKTAQPEKEVFFRLCKRFLSLPRDSEMREDILGWAINHPIGLLTEALLDWWYKQSP
                     KPGAGLPEILAPIFTKLCNPANINYRAGRVVLAARTISFFRVDEPWARQNLIPLFDWG
                     RSTEEARIMWHGFMWAGQLYRPFLEAVKQSFLDAANYYGDLRDAGPRYAEFLALAAVD
                     RGDTFTVEEMRRATSRLPLAGLVSSLHVLARSAEGAGQRQMEYWKNRIRPYLHDIWPK
                     SRRFLTEEIKRAMAELCVAAGEAFPAAYYDLKDWLYPVQYPELMLHRVKAQKIIEKFP
                     EEALDFMDKLFADDPDYHGSSLKECLDAVVKRMPGLANDGRHERLTLLLRKRGWLE"
     gene            53461..56715
                     /locus_tag="PKI64_00600"
     CDS             53461..56715
                     /locus_tag="PKI64_00600"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOD99536.1"
                     /translation="MCANHYTEDTLVQQTTAEYLEQQLGWDSVLAYNHEDFGPDSLLG
                     RLSDREVVLTRILRAKLAELNPGLPEAAYDDAVRQVTETTTTQSLVATNREKYDQIRD
                     GVQVTYRDGEGNLAKERLRLFDFRQPENNHFLCVRELWVRGDLYRRRADIVGFVNGIP
                     LLFVECKNIHRNLRAAFEQNFSDYKDTVPHLFHHNAIVMFGNGDQAKIGSVTSRWGHF
                     HEWKRLAENQPGAVDMETLLKGVCDKRNFMDLVENFIVFDDSAGELRKILARNHQFLG
                     VNRAVEAVRDRKSLDGRLGVFWHTQGAGKSYSMVMFTRKVHRTLGGNFTFLMLTDRDD
                     LDTQIYKTFAGCGVVDNDRDPCRAASGDDLARLLSLHKSYVFSLIHKFNQDVAPDAAY
                     TRRDDIIVISDEAHRTQYGTLALNMRNALPNASYIGFTGTPLFKDDEITRRWFGDYVS
                     TYDFQRAVEDKATVPLYYDSRGDKLGVSIGDLNERLADKLEELETEDLDTIQRLEQEL
                     RRDYHVITAGKRLDQVARDFVAHYSKAWESGKAMLVCIDKVTCVRMHKLIEFYWEQLI
                     RELERGLAESRDEQEEAQTRRRIEWMRQTRMAVVISEEQGEVDKFRKWDLDITPYRRL
                     IKEGIELPEAMRGLPEFQNKQRLALDDAFKAEAHPFRVAIVCAMWLTGFDVPSLSTLY
                     LDKPLKAHTLMQAIARANRVNEGKNNGMIVDYCGILKNLRKALATFAGAADGGRGDGG
                     ALPEPARPETELLADLEEALGLVRTFLSEHDAPLDNIVFRKGFARNAAIVAAKDAANE
                     NDTARKRFEIMCRAVFVKFLACTNVPDVNRYRGEYRAIRLLYQSLKEDEEKADVIDVI
                     RQLHQVVDKAIVTQDPVSAEGKEPYDISQIDFDKLRREFERSKAKNTTVQNLKAALEK
                     RLKRLLERNPLRTNFQTHYEKIVADYNREKDRVTIEKTFEELMRAAKTFDEEEHRAMR
                     EGLDEETLALFDLLRKPDLTAAEIKRIKKVAVELLQKLKAEKLRIDHWRDKETTRDAV
                     RLTIRDFLWSDTSGLPVERYTETEVQTRADEIYVHVYRAYPTLPSPIYNQQMVC"
     gene            complement(57218..57577)
                     /locus_tag="PKI64_00605"
     CDS             complement(57218..57577)
                     /locus_tag="PKI64_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99537.1"
                     /translation="MEKYLQDTRRYRLGSWFARLAGVGLLVSAFDQEDFLRILAAPKP
                     TGIFSALARRDRPLADTSPARVVRNAPSSENPPVILARQPRQNPQEILRMHVLRTADK
                     ARQGLFTITPPHDGAGC"
     gene            57860..58696
                     /locus_tag="PKI64_00610"
     CDS             57860..58696
                     /locus_tag="PKI64_00610"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOD99538.1"
                     /translation="MINRKPLEASLAAVKKAFPKAKPKLGMILGSGLSEIAEAFEQLG
                     VLPYAKIPGVGAAGVAGHAGRLVWGKSAGIETFIFQGRRHYYEGVGWEAVATPLFLLK
                     SLGAKAVFLTNAAGGTNPKWKPGDLMIIEDHINMMGVTPLLGTHDPFWGPRFPDQSFI
                     YDQQLRAALARAGKQSKVALRRGVYFAGTGPTYETPAEVRAWRKLGADAVGMSTVPEA
                     MLANAAGLRVAGLSCITNMAAGILNQALTHQEVFETSQKAMANMKKLILQFWKELGRA
                     GL"
     gene            58770..59492
                     /locus_tag="PKI64_00615"
     CDS             58770..59492
                     /locus_tag="PKI64_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD99539.1"
                     /translation="MKTMRLVCLGLGMALPWVAVADEPPAAPLAPKIVCDEPSYDFGE
                     RDNNGFVEHNYVVRNAGTLSLEIREVRASCGCTAVKPSVDVVPPGGEAQVQARFDLRG
                     RTGYQQKAIFVTSNDPETPTLTLTLKGTATQPLRAEPSTIFFGQLGPDGLRSRDVEII
                     STRGPIQIENVRSDQAAIQVTPLPAADDADGTRHRFNVTLANDLPPGQLNGVVIIKTD
                     LPDTPELTVPVLAFIVGSAPAQ"
     gene            59492..60013
                     /locus_tag="PKI64_00620"
     CDS             59492..60013
                     /locus_tag="PKI64_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOD99540.1"
                     /translation="MAESTAGRRAAFLRMLPASATLLALAVALGVAANLVRPAATRLP
                     WASDWSRHIETQAYRAGVPVLFLPGVRERAGNPDWLICDARTPEQYAAGHLPGAWNLP
                     LEEVDARLGALAARLTLQSELLVYCGGADCTDALELALKLRQLGFERVTLYPGGYAEW
                     VEYGGEVRTGDEP"
     gene            60010..60471
                     /locus_tag="PKI64_00625"
     CDS             60010..60471
                     /locus_tag="PKI64_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOD99541.1"
                     /translation="MSRARIILVWVARLAVAGMFIGACLAKIAAPESFALAVHRYRLL
                     PDLLVNAVALLLPWLELLCGVAVLAGPPRWRAAGALWIALMLVVFTGAISLNILRGIE
                     ASCGCFSTRADAAASDGWNLARNATLLGLTLVILWDALRRDQSAAAGEPAP"
     gene            60480..61724
                     /locus_tag="PKI64_00630"
     CDS             60480..61724
                     /locus_tag="PKI64_00630"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOD99542.1"
                     /translation="MQSIFEVYVAGVGPSSSHSMGPERAARLFAAGLAETPARIRVTL
                     QGSLAATGRGHWTDRTLVRTLNPVPVDVVFDSQTTNLPHPNTMIFAAFSAQGDLLREW
                     TVCSVGGGSLREADGRPVARAEPAEYPVGNIADAMDYCHANHLEFWQLVEQSERDVWP
                     RLENIWRAMTDTVKRGLASKQIILPGTLELARRAGTTHSRALGLKGTPRELGLVAAYA
                     LAVAEENAAGMQVVTAPSCGAAGVLPAVLYYFKARRRVPQRRILEALATAGLFGASIR
                     ANATVSGAEAGCQAEIGSACSMAAAAAAQLLGATLRQIEYAAEMAMEHNLGLTCDPIE
                     GYVQIPCIERNMNAALRAIECATFTQLTDGRHLVSFDDVIEVMNRTGRDLQAAYRETS
                     TGGLAQLWRRDIETRLRKPARS"
     gene            61721..62635
                     /locus_tag="PKI64_00635"
     CDS             61721..62635
                     /locus_tag="PKI64_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD99543.1"
                     /translation="MSPRVNEFSWSFSRQATFQSCLKRYYFSYYAAWGGWSATAPARA
                     REIYLLKRLATRQQWAGQHAHDALAALLQRPAADAAEAESIAQQQIEQMRQEFRQSRA
                     KAYHRDPARIPGLFEHEYNLEIPAEEWQAVVERVAQAVRQFATSELWQELQQLPREAM
                     VAVEQRAHFLLDGLKVLAVPDLVVRHEGRVTIYDWKTGSADLAEHRLQLGIYVLLALD
                     RWTSAPSDIQAVAYHPILNQREVFSYTADDVENLRDFIRDSADEMLFPVEDPMTNRLS
                     ADAEFDCTTDDNVCKTCPFLRVCPKWAT"
BASE COUNT        12392 a        19600 c        18126 g        12584 t
ORIGIN      
        1 gaacgaaacc ttcccgccgg gaaaacggct ggaaggtctg cgaccgttcc agcccgacaa
       61 tccgatgggg tcgagatttt ttcccgatca aaaaaaatcg catgacagcg gggtcaccgc
      121 ccccgcctac agtccaaccc attccgcacg ctgtagccgg tctcgctgag gccggcggct
      181 gtttctgatt ttcgcaatcc aaccggaagc ttttgtcggc ggaaatgatc cggcgagcgg
      241 gcttattggt tgacgaattt ttttgtcaaa cgccctagcc aatggcctgc ttgaccgcgg
      301 tggcgatgct gtggcaaagg cggtcggtca tttcatccgt cggcccctcg accatcacgc
      361 ggcacatggc ttgcgtgccg gaatagcgca cgaacacgcg gccctcctgc cccaattctt
      421 tttcggcagc ctgaatggcc gcttgcaccg tgggcagagt ttccaagggc gggcgctgag
      481 ccacatccac attgaccagt cgctgtggcg ccagttgcag gatggaggcc agcgtggaca
      541 tttcctgccc gctgcgccgc aaaacagcca acagttgcag gccggacacg atgccatcgc
      601 cggtggtgtg ttgctcgagg aaaatcatgt ggccggaggc ctccgcgcca atcacgctgc
      661 cgcgttcttt catcaagtcc agcacatagc gatcgcccac cgcgctgcac gccagttcga
      721 tgcccaactt gcgcatcgtg gtgtgcagcc cgaagttgct cattaccgtc cccacggcca
      781 gattgttccg caatttgccg cgttccttga ggtctttggc gcaaatggcc agcacgtggt
      841 cgccggtcag ttccacgccg gtttcatcca ccacaatcag ccggtcgcca tcgccatcaa
      901 aggcgaggcc gacatcggcg cgctcggcca gcacttgctc gcgcagcgcc tcggtgtgct
      961 gtgagccgca gcgggcattg atgttcagcc cgttgggcgt gttgtgcagc gtggtgacgg
     1021 acgcgcccag ccgcttgaac accgccgggg ccgccttgta ggtggcgcca ttgccgcaat
     1081 ccaacaccag ccgcagacct ttcaaatcca tgtccgcggg gaaagtgcgc acgcaaaaat
     1141 ccgtgtagcg ttcgagggcg tcttccaccc ggaacgattt gccgatgccg ccggccttcg
     1201 gcaacatttt gtgcagcttg ccaccagcca acaggttttc aatggcggtt tcttcctcgt
     1261 cgggcagctt gtagccatcg gccgaaaaca gcttgatgcc gttatcctga aacggattgt
     1321 gcgaggcgga aatgacgaag ccggcatccg ccttctggtc gcgcaccagc accgcaatgc
     1381 cgggtgtggg gagcgtgccg acgagtagcg cgtcgccacc catggagcaa aggccggcta
     1441 ccaacgcgtt ttccagcata tagccgctga ggcgagtatc cttgccgatc accacgcgcg
     1501 gacgtttgcc gcccttgcgc tccttgagca cataagccac ggcgcgggcc agattcaccg
     1561 cggtctccac catcatcggc tcgcgattgg ccacgccacg cacgccatcg gtcccaaaca
     1621 gcttgttctc gcccattgcg aactccttat ttttggtcaa ccccgcgggg ttgactgatc
     1681 tggcccggca gtgtgccaca gttccgtgcc gtcattcacg cctttttgtt gtgccgccag
     1741 cgcgaagccc ggcgcgcggc gactcaaagc tggccgtcgc gcagcaccag atgccgcgca
     1801 cagcgggccg ccaccgcctc gttatgcgtc acaatcacca gcgtgaggtt ttgttcccgt
     1861 accaaatcga acaaatagcg caggacatcc tcgccggttt gggagtccag attgccggtg
     1921 ggctcgtcgg ccagcagcag ctccgggtgg ttcatcaggg cccgcgccag cgccgcgcgc
     1981 tgctgctcac cgccggacag ctcgctgggg cgatgctcca gtcgctcggc caaacccacg
     2041 cgcgcgagca attcaccggc gcgttggcgc agcttgcggc cttgcaggaa tgcgccgggc
     2101 aaactcaagc cgggcagcag cacattttcc agcacggaca gctccggcag caagtgatag
     2161 gcctgaaaca caaaaccgat tttttgcgcg cggattgccg cacgggcgcg ttcgcggcag
     2221 gcatagaaat cgcgcccgcg gtagagcacc tcgcccgcgg tgggccggtc aaggccaccc
     2281 agaatatgca gcagcgtgct tttgcccgca ccgctcaggc cggtgatggc cagcgtttcg
     2341 ccggcgtgga cggccaggct cacgccgcgc agtacgtcaa gttgccgggc gcccagcgca
     2401 aaggaacggc ggatgtcgcg cgcttgaatc aactcattca tagcgcaggg ccctcaccgg
     2461 atcgagcttg gccgcctggc gggcggggat caccgcggcc agcgtgcaaa tcaccagcac
     2521 cagcacagcc accaacgcca catcgcccgg cagggtgtgc gcgggcagtt ccgccagatg
     2581 gtacagctcc gcggggagca gttgcatccc gcagcggtcg ttgagaaaat gcagcaggtt
     2641 gttgcggtag tgcagcgcca gcagccccgt gccgatgccc agcagcgtgc ccagcacgcc
     2701 ctgaatccac ccctgccaga aaaagacccg catcaccgcg cccgcggaaa accccaacga
     2761 acgcagcagg ccgatttcgc gcgttttttg caccgtgacg gtgatcaacg tgttggtgat
     2821 gctgaacgcc gccacaatcg tgatgaaaat cagcaggaaa aacatcatgt ttttttccac
     2881 gcgcagcgcg gcaaaaatct gctggttttg ttccatccat gtggtgacgt gcacgccaac
     2941 gcccagccgg tggcggattt cagcggccac ttcatcggcc gcgtaggggt cttgcgtcag
     3001 gacttggatg gcttccaggc ccgtaccgcc gcagaagtcg cgcgcgtccc acaacgaagc
     3061 cagcacgtag cccatgtcga actcccacat gccgatctcg aagatgccgg ccacggtcat
     3121 ttcgaccggc aggcggattt cgttttgatc gaggaaggtc gccggggaat acgcggtgat
     3181 ggtgtcgccg acatccacgc cgagccgccg ggccaaatcg cggccaatca ccaccgcgcc
     3241 atccgccagc gagaactcgc ccgcggtgat gctttgcggg atgcggctca cgtgggcctc
     3301 gcggtcggga tccacgcccc ggatcagcgg ggtttccacc tgcccgttgg gcttctggat
     3361 aaacatcaaa ctttgcagga acggtgcggc ggcagtcacg cccggcacgg cttccaactg
     3421 ctcgcagagg ctatcctcct cctctccgag gccgccggcc acggaaaccg tgatgtgcgc
     3481 gttgaagtcc agaatcttat cgcgccacat ttcatcaaag cccgtcatca ccgagagcac
     3541 aatcaccagc accgccacgc ccagcagcac gcccagcgtg gccagcaccg tgaccaccga
     3601 caggaaagtg cgcttcggct tgaggtactt cagcgccagg aacaatgaaa acggcaacgt
     3661 catgcggggg gcaagtcgtc cggttgcggc gcgcgagtct tcatttgggg gaacagcatc
     3721 acatcgcgca cgctttcctc gccggtgagc accatgacca gccggtcaat gccgacgccc
     3781 atgccgccgg cggggggcat accatattcg agcgcgcgca ggaaatcttc atcaagcttg
     3841 ccggcatcgc cgccggcctg ctcttcaagg cgcgcgcgct gatcgagggg atcgttcagt
     3901 tcggtatagc ccgggcagag ttcacggccg cgcacgacca gctcaaacac gtccaccaac
     3961 gcggggttgt cggggcagcg cttggccagc ggcaccaatt ccgcgggcag gcgcgtcacg
     4021 aaggtcggct gcattaattt cttttcgatg agcttgtcat agacctcgtg cgtgatttcc
     4081 gcgtgggtca tgctggcggg gatgtccaac tcccgggcgc gggcccagtc gcgcgcggac
     4141 tccacggtgc gttcgaacca atccgcgccg gcgtgttccc gcaccaaatc gtgataggcc
     4201 acaacacgcc acgggggcgt caggtcaatc ttctcaccgt cggcgggccc caccaccatg
     4261 gtgccgcaca cctcttgcgc cgcgccactg accagctctt cgaccagctt catcatcgta
     4321 cgcacgtcgc cataggcttc atagacctcc agcgcggtga actccggatt gtgcgagcgg
     4381 tcgagccctt cgttgcggta atttttgccg agctcgaaca ccttgtcata gccgcccacc
     4441 agcagccgct tcaaatacag ctccggcgcg atgcgcatgt acatgggctg ccccagcgcg
     4501 ttgtagaacg tcttgaacgg cgtggcgatg gcgccgcccg ccagcgtttg cagcacgggg
     4561 gtctccacct cgaaatagcc gcgcgcctcc aaaaagcggc ggatgaacga caaaatacgg
     4621 ctgcgggcgt tgaacacccg gcgcacctca gggttggcaa tcaaatccag gtagcgctgg
     4681 cggtagcgca cttcggtgtc ttgcagcccg tgccattttt ccggcagcgg atgcagcgac
     4741 ttggccagca attcccactg cgccaccttc agcgattgct caccggtgcg cgtggtgaac
     4801 aactcgcccg acacgccgat aaaatcgccc aagtcaatcg ccttgaatgc ggtgaacgat
     4861 tcctcgccca gcgtgttttt ttgcaggtag atttggaatt tgtcggtgcc gtcttgcagg
     4921 tgcgcgaacg tgcttttgcc catctcacga accgccacca cgcgcccggc ggcttgaacc
     4981 ggccgacctt cggcgaacgc cgcccggatt tccgccaacg tgccggtacg cgcaaaagcc
     5041 cggccatagg gcgtgtggcc cgccgcctga agcgtcgcca gattcgccaa ccgttgctgg
     5101 cgaaattcct tctcgtgtgc actcaattcc atgatggcct ctgctgctat aaactcgccc
     5161 accctacccg ccccccggtg tgaaatccag ccatttcccc gccggcgcgc cgggttggcg
     5221 cgggtctgcc ggcacgcggc cccggccgca aaatcgccgc caattttatc cgccgggggc
     5281 ctcgccggcc gccagtgcgg cagcgagaca acgttggatc atgtccgccg gcggtgcggg
     5341 aatcagcccg gaactcatca gcaattccag cgcgcggttg gccgcggcaa tatcctcttg
     5401 cgcgcgctgt tggatggccg ccggatccac ggcatggcgg gccacgccat cggctgcggc
     5461 ctgacgggcc accgccgcag cagtttccgc gaacacggct tggtccgtca tgagcggcat
     5521 aatgtagtcg ggcgtgaggc cctgccgttc ggcaaaatca gcgattgtac gcgcagcggc
     5581 aatggccatg gcatcggtga tcttccgcgc ccgcgccagc aatgcgccct tcaaaatgcc
     5641 gggaaaaccg agcgagttgt ttacctgatt ggggaaatca ccgcgcccgg tggccaccac
     5701 cagcgcgccg gcggtcttgg cggcgtgggg atagatttcc ggcacgggat tggcacacgc
     5761 aaacacaatg gccttgtgcg ccatagagct cacccaggcc agcgggatga catccggccc
     5821 cggacgcgag agcgaaatca acacgtccgc ctgccgcaat gcgacggcca cttcatttac
     5881 gcggtccgga ttggttcgct ggcacaattc ccatttgcgg tagaatcgcg ggtcgcgcgc
     5941 gatatcttcg cggccgcggt gcagcccgcc gcgtgaatca aacagcgcca gtcgtgccgg
     6001 gtccgcgcct gcctgcatga tcaggctggc gatggtggtg ttggacgccc ccgcgcccaa
     6061 gaagacaatc cggcaatccg ccagcgcttt gcccgccacc ttgagggcgt tcagcagccc
     6121 ggccagcgtg acgcaaccgg tgccttgcgc gtcatcgtgc caaacgggga tttcgcattg
     6181 gtcccgcaga ccatccagta cgcggtagca gttgggttgg ctgatgtctt ccaagtttac
     6241 cgccccgaag gacggcgcca gccgccgcac gaaatcaatc ataatttcgg gatcatgctc
     6301 cccccgcgca ttgcggctat ccatgcacaa cggcaccgcg tccaccccgc ccaggatttt
     6361 catcaggaag gccttgccct ccatgacccc gaggccgccg ggtggcgaga cgttgccatc
     6421 gcccagcacg cgggtggaat cgctgaccac cgccaccacg ttgccgcgcc aggaaagctc
     6481 aaacgactcc gccgcgtggt cgcggatgcg ggtggacacc gcggaaacgc cgggcgtgta
     6541 ccacgcgttg atccacgcca gccccggcaa gggcgcgcgc ggcaacacct gaatcttccc
     6601 gccataaaac cggtgggccg ccagcgccaa cgcctgaaaa aaggccgttt tggcgcgtgc
     6661 cgcctgttcc tcgcgccagt gggcgggaaa gtggtcggct atctggtcca aacgaatatc
     6721 catcttgtgg gttccccgtg ctctttatgc aaacggaccg gtttgggcaa cccaaaaccg
     6781 gtccggcaac tacgcgctgc gcgcgggaat tagaacgaaa ccacgacgcg acccgcgccg
     6841 tacacgtcca cattcatccg cgaggcgccg cccacgtagc ccatcacgga cacgtgtttg
     6901 ttgaccttgt aggccaagcc ggcgtggccg cggaacgcgt aatcgtctgc cggatccacg
     6961 gggtcatcgc ggaactgcac ttcgccgagt acgctggcgc gctcatccag gtcccagatc
     7021 agggagaggg agccggcggt cagatcgtcg cgctcgctgg gattggtgtc ggaaacggcg
     7081 ccatccatcg tataactcac atccgccgcc cagtgcagca catccatggt ggtagtgccg
     7141 acggacagcc cgaaacggcc ctgggtttcg ccggttccga ggcccttgtc ttcatcgccc
     7201 gtcgggaaga tggccgtagc gtgcgggata atccacgcgt attcaaaaat atcctcgaag
     7261 aacaggaatt gcgcgccgag cgagacatcg ccgaggcctt tttcgtccaa ccggttgtcc
     7321 gaatagccca cgaacggcac gccgcccaca agagccacgc gatctgtcag cccgaagcgc
     7381 acgcccgccg tcacgtcata aaacttgatt ttttccgcac cgcgaatctc gggatcatag
     7441 gaggagccgc caccgcccaa ggacacttcc gccctcaacg cccccggaaa tttgttttcc
     7501 atggtgaaaa ccgtccgcat cggctccgcc gacgcgcaag ccaccatcgc acccaaagcc
     7561 accgcggcca acaacccttt acgcacgctc atcgtcattg ctccttgttc gccggcgcca
     7621 agcgccgagg tttatgattc gggcaaatcc taccttgttc gcccgcccgc tggcaatccc
     7681 ggaatttggg ttttacttga cgacggctcc gctggaaaga tcgccatcca ccgtcaccag
     7741 ccgcaggcgt tcgcccttgc tggccgccag cagcatgccc tggctctcca cgccacgcag
     7801 tttgatcggt gccagattcg ccaccatgat gaccgtcttg ccgatcagtt cctcgggttt
     7861 gtagtagagc gcaatgccgg ccacgatctg gcggcgttca tcacccatca acacattgag
     7921 tttcagcagt tttaccgcgc cttcgatggg ctccgcgccc atgattttgg ccgtgcgcaa
     7981 ctgcaccttg gcaaaatcgt cataggtgat gatgccttcc gggttgccga tggtgccggg
     8041 gggcgcggcc ttgggggccg ccggcgcggc cgggggcgtt gccgggcgct cgccatcggg
     8101 caccggcgcc tcgatgcgcg ggaacagtgc gccgggcggg gccacggcga cgtcgggcgg
     8161 caggatgccg ggggtggaca attccttggg atctacagtc atggggttct ccatcccgag
     8221 tgcggcgcgc agctcggtca ttttggcggg catcacgggc atgagcaatc cggcgcaaat
     8281 gcgcagggct tcggcggccg cgtacaacgt ggtgcgcaag gggcctgggt ccgccgcgcg
     8341 ggcctgggtc cacggttgct gggtttcaat aaaacggttg gtggcgcgca cggcctcgat
     8401 cacctgcgcg atcccggcgt tcagcgtgaa gttttcggtg gcagtccgga agctggcggt
     8461 ggccgcagcc acctgccgga acaactctgc ggccggcccc ttttggaaag cgcccgtttc
     8521 cggcgcgggc agccgcccac cgcaatactt gccgatcatg ctcaccacgc ggttgagcag
     8581 gttgcccaga tcgttggcca agtccgcgtt gtaacggcgg atgaagccct cttcggtgaa
     8641 cgaggcatcc tgccccagca ccatctcggc catcaggaag tagcggaacg catccacgcc
     8701 gtagcggtcc atcaactcca tcgggttgac cacattgccg agcgttttgc tcattttggc
     8761 ggagcccatc agccaccagc cgtgggcgaa taccgtcttg ggcagcggca cgcccagcgc
     8821 gtgcaacatg cagggccaat agacggtgtg cgtggtcaga atatccttgc ccaccagatg
     8881 aatggccggc caccagcgcg caaaggtggc gtcgtcgcgc cggtatccga tggcgctgat
     8941 gtagttgacc agcgcatcga accagacata ggttacgtat tctttgtcga agggcagttc
     9001 aatgccccac gacagccgcg atttgggccg cgagatgcac aaatcttgca gcggcttgcg
     9061 caaaaaaccc agcgtctcgt tgcgccggaa atcgggttga atgaactccg gatgagtctg
     9121 gatgtagtcc accagccaat cctgatattg gctcattttg aagaaataat tggcttcgcg
     9181 cacgcgctgc accgcgcggc cgcactccgg gcagttgttg ttgaccaggt cccgcgcggt
     9241 gaaaaaccgc tcgcacggca cgcaatacca gccgtcgtac tcgtcgcggt aaatttcgcc
     9301 gcggtcgtac aagtcttgca ggatttcctg cacaatgatc ttgtggcgcg gctcggtggt
     9361 gcggatgaaa tcgtcgtttt ggatttccaa ccgttgccac gcttcccgga aacgcaccac
     9421 ggtgttgtcc gcgtggacct gcgggtccac gcccagtttg gcagcggcct cctgcacttt
     9481 ttggccgtgc tcgtcggtgc cggtcaggaa ccacgtaggc cggccgaaca ggcgttggct
     9541 gcgtgccagc acgtcggcca gcaccgtggt ataggcgtgg ccaatgtgcg gccgatcatt
     9601 cacgtaatag atcggcgtgg taatgtaata ggaatccggt ttcatccaac gctcctgtcg
     9661 gcggagtttt gtaccgcacc ccgtggagcg gtgcaaatca aatcaagatt cacccaacgc
     9721 catccacacg gcattgaccg tcaagcgcgc gagttcgcct tccggcgctt gcaagatgcg
     9781 cacgcccccc cgcccgcgcg gcgcccatgt ggccggattg gatgggccga agagcgcggt
     9841 gaccggcagc ccgacagccg cagccagatg tgtaatgccg gaatcgttgc ccaaaaacgc
     9901 ggcgcattcg gctagcgtcg cgcctagttc cggcaacgtc aggccagaca aaaacggcaa
     9961 atctggaagg ttccgctgca acgctgcggc ttcgtcgaga tccgcctcgc caatgacggc
    10021 caccacttcg cggccctgcg cgcgccagcg agtgatgatt tccacatagc gcgccgccgg
    10081 ccagtttttg tcggggttgc cgctgcccgg atgcaccacc agcgggcgtc cgcggatgcc
    10141 gcggtcgcgc agtcgtgccg caccttgcgc gcggagttcc gcgggaaaat ccagcacggg
    10201 atgcgggtcg ccgtcataga tggccagcgc ttccagcggt gccagcaaaa aaggaatggc
    10261 gtggccttcc ttgatgatag gcgaaccgct cagtacctgc ttggcgcccg ccagcagcag
    10321 gttgctgcgc acctgcccca ccgggtcatg tagataattg aacaccaaat caaacgaccg
    10381 caccgcagcc acttgggtgg gcgtcaggtc tggcttgggt acaaaaaaac gcgccatgtc
    10441 cgcacggtcc agcgagacga tgctctgcgc caagcccacg gccagcgcca gctcggcaat
    10501 gtgcggataa ccccaaatct cgatgtgcgc cgccggccag cgggcgcgca acgcctgcaa
    10561 cacgggcagc gtggcgatga agtccccgat ggcgccaccg cgcaacacca gaaaacgcgg
    10621 ggcatgggat acgatggcct cggccatggc gtaaagctta gcgcaggtga gccacgccgg
    10681 cgatcaacag aaccacgcca gccaccaact tggccacggc cagcacgcgc agtccttgcg
    10741 gatgggccag cggcttggcg gcccagcggc ggaaaaacca cggatacaac accagcaacg
    10801 cgcccgccac cacccacgcg taggcaatcg tcacgaccac caaccgccat gcggaatcgt
    10861 gccagcgcac gctgttgagc atggggttgg ccagcagcag cagtagccca cccaatgcgc
    10921 gcggtgccag cagttcatcc agcagccaca caatcagcac aaacactacc acgcccagca
    10981 ccggcacgag cggcttgaac ggctcaaacc aacccaatgc ggcatgacgc accacccacg
    11041 tcacccagaa cacatccacc gccgtcaacg cccagcccgc ccatttggcg cgcgggaagg
    11101 cttgcagcat gcggcgggac gcgccggggc agcgccaaag caccgcgccc accagtacgc
    11161 tcaacagccc gagcagaatc agcaggtcac caagataccg ggctggtgcc atcgtcttgt
    11221 cctcggctgg gcgcggggcg ggttttcttg cggggggggg cttttttgtc cgcggccttg
    11281 cgccgggcgc ggacgtcttc caggctcttg gggaacgggt gcgccggcgg caacgccggc
    11341 gcgaccaata tttccgggtt gtccggatcg gtcgtgaaag gcgtaccgcc ctgcacttcc
    11401 aagtcgccgg tgtcggtgcg cgcaagtttc atggcgccgg ccgtgcccac gtagagagtg
    11461 gcgcccagca gcgcgccgtc ataactgaat tgcgaatgca caaagcggtc cggcgcggct
    11521 tggtgcagcg tgtggaaaac gccattatga tccaactcca gcgtcggcac aaaaaagcga
    11581 atcacccggc ccagttcata cgcgccgaga cagatccccg cctgcggatt atccagccgg
    11641 aaaaacagcc ggtcgttacg gtcgcgattg agttgccgca gggagacttc ccgcgcggag
    11701 ggggtgttcg gcacgccgta ggtccgcttc aacaactcca accccggctg cacttccaga
    11761 tattgccgct cgccgatgaa tcgcgttccg gcaaactcca gcaccggggt caccttgtag
    11821 gtctggccct tgatcagtac ataggcttcg agcaaattca catccacatc gcgggtttcg
    11881 ccggcgggaa tcaggatggg ccggggcagc aacgggcgcc ctgtggtggg aaccggcgaa
    11941 gtgggctggt cttccacatc aaatgatagc cgcgcgttgc cgcgcggcgt aaagtccagc
    12001 gtcacgcccg aattgttcac cacgcgcacg gtggccagca ccggctccat cagcagggtg
    12061 cggttatgcg ccaacgacca cgacaactcc acctgcgccc gccccggcgc cgcccacagc
    12121 aacgcgccca ccagcgccaa gccccacgcc atgctccgct tcatgcgcgc ccctaccgcc
    12181 cgatttccag gcgtgatgcc gcgcgtggcg gcaggcccgc atcgcggatt ttcttttgcg
    12241 caccggcaat gtcatactcc acgcgacgca actccacgac gttttgcacc aggtcataaa
    12301 tcgcgtacgc cgcgcgcggg tcgccatcgc gcggctggcc gacgctgccc acgttgatgt
    12361 aatacttgcg gccgccggtg atcagcagct tggtataaag cccgccgcgc acgatgccgg
    12421 ctttctcgaa tgccagcggc acgtgcgtgt gcccgaagaa gcacaccgaa cacatctggt
    12481 aggcaaagtt ggcctcggcc tccagcttgt cgaacacata gccccacatg tcgggattat
    12541 ccagcgtggc gtgcaccagc atgaacgtct cgaccgtgcg tgaataacgg agattcccga
    12601 gccaagcccg ctgttcatca tccagtcgct tgcgcgtcca atccaccaca tcggctgcca
    12661 gcgggtggaa gccattgagg ttctcctgcc gcgagcagta atggtcgtga ttgccgcgca
    12721 cgacgttgtc gccgcacaac tcccgcacct tggccaggca cagctccgga tcagcgttgt
    12781 agccaaccac gtcgcccacg cagcagtagt gggtcacgcc ctgtttttcg gcatcggcca
    12841 agaccgcact caaggcctcc cagttggcgt gaacatctcc gagaatggcg tatttcagat
    12901 tcatggcgct actccggctg caattcgctg cttggcgtgg ccggcacgga ctccggcacg
    12961 ggctcggtca cactttgcac cggttctgcc gggagcgcag gctcgctaac gtcggccgcc
    13021 tccggcacaa tttcggccgt tgcctccggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn tggttccgcg gcaagctctg ccggatcgcc ggtctcagtc accgtcgggg
    13201 cggctggctc ggctttgccg ccggttgccg tcggcttggc ttcggcgccg cctcgcggca
    13261 gggccaacgt ggcattaaag aattcaatca cgccgcccgt ggccacctgg ttgctcaaat
    13321 gcagcagcaa ttctgcggca tctgcttctt cgggattgcg gccgggacgt agtccttcca
    13381 gcacttgctg gcgggtgacg ccgggatgct gttcaatgta gtccacaatt tccttcacga
    13441 gcgccggcgc ggtgttcggg tccaccggct tgggcagtac ggccgtcaca tacgtctcgc
    13501 gcgcgttcac cttgaacagg tgtagccgca tgtggcggaa tgccggccgc aacgccagcg
    13561 agagtgtgaa cgggaagcga ctttcgcgct gccacgccaa ggacaccgcc tgccgcagcg
    13621 agccgtcatc aaactttttg gactgatggg ccggcagtag gcagcgcccc gattcgcgca
    13681 aaaggccatt caccttgccg gcgctcatcc attcctgcgc ctgcgcacgg gtccatgttt
    13741 cggcttcctg cccttccggc accgtggtca gccggtaggt ggtctttttg cgcagcgagt
    13801 ctttccactg ctccaccagc gcttcatcgc gcacggtttc gatgtggccg agatactccg
    13861 ccttggacat gtgcgcaaaa cggcgcgacc acagctcctg gatttgttcg ttgtagccgt
    13921 ggtggttggg cggccccagc agcgtgccgc tcaatccgca ccgcgccacg aacgtaaagt
    13981 tgcccgccgg tggttcacaa tccacttctt caatcgtgaa aaacttgtcg aggtggtcat
    14041 gcagcacatg tgcttccgca ttcgcccgat gccggaacac gcgcttgcac tccagacatt
    14101 gcaccagcac cgggcgcggt tggccggctt ccgccggcgg catttccaac ttcaccaagt
    14161 acgaatcctc gcggctcaaa aaccgctggg cgatttcaat caacggatac gcgcggcggc
    14221 tggcctgaat atcgcgcacc accagcgcca gccgttcgcg gtccggcaaa aaagatactt
    14281 tcacgttcag ccgcggcagc gcctcgcgcg gcggccggcg gtcatcacga cgtcgttcgc
    14341 catcgcgacg cggaccgcgc cgatcctgct ctgcgccgcg gggtggtcgc ccgccttcgc
    14401 gcggtggccc cgaccgccct ccgggccgcg gacgatcttc acgccgcccc ccgccccaac
    14461 tgccgccatc ccgccgcggg ccgcgccgtt cgcccccttc gggccgccgg aacgatcccg
    14521 gacgccggtc ccgcccgccc ggccggcggt cacgctcgcg gtcgcgctcg tactccccgc
    14581 catcctcgtg ccgcgcatac gggttttccg gcggggtccg ggcccatgtc ggcacaaaat
    14641 tcaaatccaa cgcaaggtcc ggtttattat cctgcgcctg tttctccggg ctggcctctt
    14701 cgtccatgtt catgctcatt tcattgcggg cccgcttgcg cgccgcgggc ctctctgaag
    14761 ccccctcgcg ggaacttcgc tcgttccttt cccggccgcc aacaccacgg ccatcagcgg
    14821 acttttctag catagggcgg acacactggc aacgagcaac tggcggatac gaaaaaagcg
    14881 gggcgcgttc agcacgcgcc ccgcggaggg aaaccgggtc ggtttagtac atgtcgccca
    14941 tgccgcccat gccgccgccg ggcatcggcg gggtggcttc cttcttctgc gggatctcgg
    15001 ccaccatggc ctcggtcacc agcagcaggc ccgcgatgct ggccgcgttc tgcagcgcgc
    15061 tgcgcgtgac cttggtcgga tcaatgatgc cagccttgat catgtccacg tactggccgg
    15121 tcgccacgtc gtagccgtag gcgcccttgc cgttgcgcac atcctgcacc acgatggagg
    15181 catccaggcc cgcgttctcg accaactgcc gcagcggggt ctcgcacgcc cggcgcacga
    15241 tatccacgcc gatcttggca tcgccggaca ccttcaggtc gtccagcgcg gcctgcgcgc
    15301 ggatcaacgc cacgccgccg cccggcacaa tgccttcttc gacggccgcg cgggtcgcgt
    15361 gcaacgcgtc ttccacgcgc gccttctttt ccttcatctc ggtctcggtc gccgcgccga
    15421 cattgatgac ggccacgccg ccggccaact tggcgagccg ctcctggagc ttctcgcggt
    15481 cgtagtcgga ggtggtttcc tcgatctggc ggcggatctg cgcgatccgg ccctggatgt
    15541 cgctggtctt gccggcgcct tccacgatcg tggtgttatc cttgtcaatg ctgatccgct
    15601 tggcgcggcc catgtccgcc agcgagaggc tctcgagctt gatgccgagg tcttcgctga
    15661 tgaacttgcc gccggtgagg acggcgatgt cttccagcat ggctttgcgg cggtcgccga
    15721 agccggggct cttcaccgcg cacacttgca gcgtgccgcg cagcttgttc accaccagcg
    15781 tcgccagcgc ttcgccttcg atatcctcgg caatgatcaa caacggcttg ccgctcttgg
    15841 ccacgttttg cagcaccggc agcagctcct gcaggttcga gattttcttc tcgaacaaca
    15901 ggatgtaggc atcttccagc atgacttcca tcgtgtccgc cttggtcacg aagtacgggc
    15961 tcagatagcc cttgtcgaac tgcatgcctt ccaccacgtc cagcgtggtt tcgatgccct
    16021 tggcttcttc cacggtaatc gtgccgtcct tgccgacctt ctccatcgcc tgggcgatga
    16081 tcttgccgat ggccatttcg ccgttggcag aaatggtgcc gacctgcgcg atttcctcgt
    16141 tatccttgac cttcttgctt tgcttctgca gcgcggccac gacggcgtcc gtcgcgcggt
    16201 caatgccctg gcggatgccc attgggctgg cgccggcggt cacgttcttg aggccttcgc
    16261 gatagatggc ttccaccagc gcggtcgcgg tcgtggtgcc gtcgccggcc acgtcgctcg
    16321 tcttgctggc gacttcgcgc accatctgcg cacccatgtt ttcaaacggg ttctccagct
    16381 cgatttcctt ggcgacgctg acgccgtcct tggtaatcgt cgggctgccg aacttctttt
    16441 caagcaccac ggtgcggccg cgcggcccca gcgtgctctt gacggccttg ctcaacttct
    16501 cgatgccctt gagcaccaat tgccgggcct cggcatcata aacaatctgc tttgctgaca
    16561 tgttcgttta ctccttgcgt accttgttta ttccaggatg ccgaggatgt cttcctcgcg
    16621 catgatctgg tattccttgc cgttcagctt cagctccgtg ccgccatact tcggcatcag
    16681 aaccttgtcg cccttcttga cgttgaacgg aatcttcttg ccggtgtcgt ccaccttgcc
    16741 ggtgccgacg gcaatcacga tgccctgctg gggcttttcc ttggcggtgt cgggaataat
    16801 gatcccgccc ttcttcactt cctcttcctt cacgggctca acgagcacgc gatcacccaa
    16861 tggttggatc ttcatggtct aggtttctcc tgtttggttg aacatggccg gcccgccgcg
    16921 gactcccgcg gccggccgga ccgtttgttt ttttattttg aattcttctt ctggtcgtca
    16981 tccaccatct cgaagtcggc gtccatgacg tcgccatcgg ccttggcctg ctcgtgcggc
    17041 ggtggcgggg cgctgccgcc ggcggcattg ccgtccggcg cctgctgctt ggcctgcgca
    17101 tacaggtcgg cggaaaccgc ctgcatgtcg ttggtcagct cctcgctggc tttcttgatg
    17161 gcgtccgtgt cgccgctctg gatggcggtc ttcaacgctt gaatgccgtt ctcgaccttg
    17221 agtttcttgt cggaggggat cttctcccca ttgtccttca ggaacttttc ggtttcatac
    17281 accatggcct cggcgcggtt cttggtgtcc accgcctcgc ggcgctgggc gtcctcgccc
    17341 gcgtgcgctt cggcatcgcg caccatccgg tccacctctt ccttgctcag gccgctcgaa
    17401 gctgtgatgg taatcttctg ctccttgccg gtgccaagat ccttcgcgct aaccttgaga
    17461 atgccgttgg catcgaggtc gaacgccact tcgatctgcg gcatgccgcg cggcgccggc
    17521 ggaatgccat ccaagtggaa cttgccgatg gtcttgttgt ccgccgccat cttgcgctcg
    17581 ccttgcagca cgtggatttc caccgtcggc tggttgtccg ccgccgtgct gaaaatctcg
    17641 ctcttcttgg tcgggatggt cgtgttgcgc tcgatcaatg gggtcattac gccgccgagg
    17701 gtctcaatac ccagcgtcag tggggtcacg tccagcaaca gcacatcctt gacttcgccc
    17761 ttaagcacgc caccctggat cgccgcgccc acggccacga cttcatccgg gttcacgccc
    17821 ttgtgcggct ccttgcccag ttgcttttcg accacttgct gcacgcgcgg catccgggtc
    17881 atgccgccga ccaggatcac ttcatccagc ttgtccttcg gcagctcggc atcgcgcaga
    17941 cagttctcta ccggcgtcat actccggtca atcaggtcgc ccaccagttg ctccagtttt
    18001 gcgcgcgtca acgccacgtt caagtgcttc ggcccgctcg catccgccgt aatgaacggc
    18061 aggttgatgt cggtgctctg cgcgctggac aactcgcact tggccttttc cgcagcttcc
    18121 ttcaggcgtt gcagcgccat cgcgtccttg gacaggtcaa tgccgttgtc cttcttgaac
    18181 tcggccacca gccaatcaat cacccgctgg tcaaagtcgt caccgccgag gtgcgtatcg
    18241 ccgttggtgg ccttgacctc gaacacccca tcgccgattt ccagcacgga gatgtcgaac
    18301 gtcccaccgc ccaagtcata gaccgcaatc ttctcgtcgg acttcttgtc caggccgtac
    18361 gctagcgaag acgctgtcgg ctcgttgatg atgcgcagca cttcgaggcc ggcaatgcgc
    18421 ccggcatcct tggtggcctg ccgctgactg tcgttgaagt acgccggcac cgtaatgacc
    18481 gcctgcgtga tcgtctcacc caaaaacgcc tcggcatcca gcttcatctt ctggagaatc
    18541 atcgaggaaa tttccggcgg cgagtacgtc ttgtcgccga tcttcacgtg ggcgtcgccg
    18601 ttggccgcgc ggaccacttc gtagggcacg cgcttggcct cttccttcac ctcgtcatac
    18661 ttgcggccca tgaagcgctt gatggaaaat accgtgtttt tcggattggt caccgcctgc
    18721 cgtttggccg cgctgcccac cagccgttcg ccggttttgg tgaacgccac caccgacggc
    18781 gtcgtccgcc cgccttccgc gttcgtcacc accgtcggct gaccgccttc catgaccgcc
    18841 atgcaggaat ttgtcgtccc caagtctatt cctaatactt tagacatcgc taacctcttc
    18901 tttccttaaa aaatcctttc aactattaca caacttcccc atctatcaag cagcaaccat
    18961 gccaattcgt ttgatatatt tgtaattcat tgccattcat cttgttatat aacatcccca
    19021 tctcaagttc tccgcccaat ttcacctaaa tagcgccata atgtcacaca acaaggtcaa
    19081 ccctgtcact attgctcccc cccccccccc cccccccccc cccccccgct caccacccgc
    19141 catcagtatt cactcacgca caacctgtgg cggctggtgc cggaatgcct tggtcgaata
    19201 tttccacatg tggggttttg tctttttgac attggaggcg gcttgtgcgc agggtgcccg
    19261 tgtgccgacg gcttcatatt aggatgcgat gggcaggccc ggacaccgcc acgacgatcc
    19321 cgaccaccca agaagttgcg cttcccgtct gtcccttgcg gatgatcccg gaagccgcgg
    19381 ccaccccgcg gcaatccccc gctctgcccc aatcactttt tcaaaaattc acttgcctca
    19441 tagaaggcac ccgggaactg attgttaggt ctcagtttgt ctgggcgcaa ctaccaagac
    19501 ccgtcgccct cttggtgtcg ttgcgtcgat tattgtaggt gttgcataag ggggatttgc
    19561 aatcacgaga acaccctgat caattccgct tatactgaag tagcggtcaa gttgctcaag
    19621 cccggatcga accaactgac gagagaaact cgttcctttc agttcgacta aaagtttttc
    19681 atttccgcgg ctgagtaaaa ggtctcctcg gtaatgacga tcagtgccca gtagaacctc
    19741 aatctggaca ttcaactttg gagcgatgga ctcgatgaat cccgataggg agcccagaaa
    19801 ttcagcctct cgcattcgtg gttcttcgtg ggccggcttg tattgagaaa taaattcaga
    19861 aataagattt gcggcgaagc tggccaagtc agttcctttt gctttcagcg ccttcgcaat
    19921 tctcatgcgc tccttctttg actccagctt agaacggtcg tgtgcaagct tctcaatgat
    19981 ttgatcgaga agaacatttg taaatgcaca cactgagacg atcgctagaa aggcctcact
    20041 tgcatcgaag tcgagcttgt ggtcatgggt tgatgggttt ctccattccg tccggtaatt
    20101 tttgaattgt cttaaaaccc gtgacctaaa gacgtcgttc gacgcaaaat gttcttcaat
    20161 attgtgcggc gttttcttat caggatcctt tccagttaga acacgaaaag cctctttcag
    20221 actgccttca aaagcttgat tggttcggta aatgcaatca gtaaaggccg tctcgtccgc
    20281 cgcatcttgg ccacggtcaa aatgtgctac agccgtcacg atatgacgca agaccgcctt
    20341 gagtccaagg aagtggttgc cttccccagc cctctcgatt tttgatcgta tgatggctac
    20401 aagatccata cttttgacct aacgttaaca gcgcattgtt gggacactac ttctccccgt
    20461 tgattgactt tatttctgga tagaatttct ctttcagtcg ctaagtaact tcgggtattt
    20521 catgggttag ttctaaaagg agcttttctg cctcctcggg tgtttgtaca aggccccgag
    20581 gggcattttt tgattatgct tgatttgagg caactgggat agatagcccc ggggaattat
    20641 tgcagggata cccaaataat tcttgccgaa ttatcatata ttcattgagc atgctgttgt
    20701 tagggtgcat ggtacggccc caagaatcga gccccgttaa agtggatgag atgcgagggc
    20761 gcatcagcca cccacacttc ggtttcccag gcgatctcgc ccaagtagcg gcccatgatg
    20821 gcccggtttg ggaaggcggt cacatagacc agtccagcgg tcgatccggc gaatagcttt
    20881 gccaactcgg cgtgacgctt gccatcaacc ggaccgtgac tggtgacgga ctcgaccagc
    20941 agcagccaat ttttcgtgat gaagtgcagt accacgtcag gcatcttgcc atgtgaatcc
    21001 acattgacac ccaggccggc cagcaaggat gcatcgaagt aaccccactt gtcgcctgtg
    21061 tcgccggcgt agaccagcac gctgcccggc gcgaagcgtg gggcgaagtc ctcgatgaca
    21121 gcccgaatca gctcgctatg ttcgccgggg ctgagggtga ttttcttgcc cggcgcaaac
    21181 ttgactggga tacggttctg ctcgcgcttc ttggcgtagc gggcaaccag cgtttcccgc
    21241 tcggccagat aggcagtcag tctgtcatgc catgccggtg taccgaaagt gcgcagcaac
    21301 gccagcgcgg ccgcttcgat ctgatatact gcctttggac tgttcactgg ccggtccggc
    21361 ctgtcagggt tgtaaagggc gatgccggca tcgcagaact gatgcatggt ctgccgacgg
    21421 aacgtctcgc gggtgttcgg tgcgtactcc ttgccgtagt gctcgcgcgc ccagttcatg
    21481 ataggcgtga tacccataag cgggttttcc gcgccagccc acgacttgcc cggcgtgagg
    21541 ttcagcaggg ccagcagtga gagcgcggaa cgctcgtttt gctgtccctt cggcaatcca
    21601 agcgaaacga gaatctgatt tgcagccttg atgtatttgc tcttcttgct ggctctcata
    21661 tcgcaattcc ccagaacttt tcgtcaatca tgctctgcgt cagctcccct tgctgcattg
    21721 cccactcccc aagcttgatc aggatctcac ggctcggata cttcatcgcc ttgaggtcgg
    21781 ttgcattgac ttgggtatgg ccattgaaac gcctgaatat ctcatcagcc gcggtcgtgt
    21841 tcaggtacac ggccaaccca tgggccagcg cctcgggcaa ccctcgcttg ttctcatgga
    21901 acaggttcaa atggttctca aagcccagta cgggcacgtc accgaaggca ctcggcttga
    21961 ccacgttcgc cactattcgg cgcttttctt ccttggacga gaagcgccga actacgcagt
    22021 agaaaccact agggtacagc cacttttcgg tgtcggcatt gcgctcgatg gcgttgggct
    22081 tcttcatgcc ctcgatgggc catgtggtgc cggtgctgct taagtggccg ggatacagca
    22141 agggaacgct gccctcccct ggcatgtcac gcaagtgctc tttcaggcgg aaatcaacca
    22201 ctggcccggt ggatacattg atgcacagat cggccagcgt atagcagatc gtctctgtca
    22261 gctcaatggc gtgtttctct ggggtcgtgg gaacgtgaat gaatcgttcc gggtcatcgg
    22321 ggaacacgat acggccgaac gggtgttcat gggtctcaag atcggcgaag gtgtcatcgg
    22381 tagaggtcga aaccatcact gacccctgct ggccaccccg ctccaggcgg atgatgatgt
    22441 tctcttggag aaccgcgtcg tccttgaatg ccgcgctgcg cgactcgaac aggtgcatgt
    22501 gacgaatggc tgcgcgctca agaatgaagt cgcgaaacgg ccgataatac ggcccattgc
    22561 agaagctgcg cggaataatt gccacgatct gtccgcccgg cgcggccaga gcgacagcca
    22621 gcgcaatgaa ggcgctatac aggttcaccg tctcaatacc gatactacgc aaggctagcc
    22681 gatgcgcgga attgctattg atcttcttat agggcgggtt gaggatgacg tgagtgtatc
    22741 gtggaagtaa ctttgagaac aggcccccgc aaagtgcatc tgtggctgtg tgaatgaaat
    22801 cgtctccatg aaccgcgaca gagagccgag aactcgcgaa cttgtatagt gtcctcatca
    22861 actcgctatg cagcgaatca tcgatctcaa aagcatcgac ttctacacgc tggaagccaa
    22921 agccaccgaa agaccatcgc tccaggaaag cagccgcaag agatccaata ccggcacccg
    22981 cgtcaagaag gcgacatgtg ccatcggcgg atgggaatag accggccatg aaggccgcag
    23041 tccccccagg cgtcaagaac tggccatact gcgacttctt tttcgcatcc gtgttcttgg
    23101 atagacgcat ccgggcttgt tcaacatcta acagcaatca atcgtctcct atttcacatt
    23161 cgaacgctag cgatgaggtt gaggcggctt gccgtcggac ctcacgcgcc ttgttcaaca
    23221 acttcatttt tacgtgacgc ggacgattga gtccagaata ttggtgtttt agcgtcgcaa
    23281 acggctcgtg ttcggctttt gaggtcattt tggggtgttt tgggctattt tcaggcgcaa
    23341 aaccggcatt ttttctcatt tcctgccgtg ccgcgagaca aggcgcacca gattaggcag
    23401 atctcagcgg gacttgaggc ccctcactag gcgcgaactc accacaggtg tgcggggcgg
    23461 tgattattcg atcatctccg cccttaccac ccaaagaatc ctatgttggc cacgaggcaa
    23521 ataatcatca gggcgccggg gatgcccccc gctagcaaca tggctggtgc cacatgcttt
    23581 agcctagcca agttattgac aatcccccac cgatataaca tccaacgctg ggcaagtacc
    23641 aaaaaggtcg cgtaaatcca gataacgcct tgtgcaatgc cggccacaaa gccgaccggg
    23701 ttccgcaaaa gaataccgac aaatgagaga aggatgagaa cgccttgcac ggctacatcg
    23761 gcgaccgcct ccccttgcga gatagccccc atagtcctct ccaagggatc ctgacttctt
    23821 cgcgaatctt cctgcagacg aaggcgcaat gccgcgtccc agttgaatag agataatccc
    23881 tggccaaaaa tggtaaacac cccgccgccg acaaacagca tggccagcac tgcccaaatt
    23941 cctacaggaa acgacccaac ttgatgcgtc agaatataca tatactccgc cttttctgtg
    24001 tcccgaacgt ctaaggcatg cgggctgcca aacagtccac gggttgtcag tacgcacgac
    24061 cctctggtag ccgtttatct tagtctctga tactgtcttt gcgcactcgc cagtactcga
    24121 gcaaattcgc tttttaaggc atgctccaat gcctgtacat agttatcagt ccacaaatca
    24181 tcggcaccgt atttcttcgc atgggcaatc cattcttcct ggcattgtcg gatgtatttt
    24241 aggaatagac tagccctctg gactctggcc tccattcttg catagccacg actcgcttca
    24301 atcttctgcg ttgcttcaac gagaccatcc caaaatacat cgtcatgaac gcacgcatca
    24361 atcaggcagg attcaaagta gtaaaatcgg gacgacaact cctgaacgag ataggcccct
    24421 agcctcgtcg ccaaaatggt ggcatcatca tccattggtc gcccatcacg cgtatttatc
    24481 aatccatacc cgaggagatc ctgcaccagt gtagcaccat ctttacgcga gactccacag
    24541 gaatggagaa actcaattat agcacgcgcg gattcacccg aaaagtcctc ttttgatccg
    24601 agtcgcttta ggcgtgacac aagtaagtat ctcagcagtt gtgtcggtac cctcccgtaa
    24661 tgagagtcaa aaatgtttgg aatcgttgac tcttcctctc gccaatactt gcgctgagca
    24721 aatatcgcgc ctttgtaaaa ttcgtgaggc gcgaatttga aagagcctgt tcgagcgaac
    24781 tcccacagag ccttatctgc tgatgtatgg ccgcttgcca tgaaattccg cacaagagcg
    24841 aggccacgac gaacgttgtt gcccgacagt acctcaagca taaaaccagt ctcttcggac
    24901 agaatcgaat cagtcaaaat atcgaagaat gagcctagat catccacaac aaagcgatat
    24961 ccactttcgc caactatcgt ggccttcttg ccggtgataa acttctttgc atatgcaaaa
    25021 cggcgcgaga gaactggaac gacctgtggt gggtctatga acagtgtgtc catctgaaac
    25081 gcgtcgaata cgggagttgt catatgccgc aggtaagttg aatcgcgtag gctcaggatt
    25141 gcatgcatcc ccatttcacg agcagccgct tgggtttcca aaaagacacg cttctgatat
    25201 tcgacgtcct ctatctgatc gacattatca accacaagga atgacggtgt gtgcgaggta
    25261 acgtgtctca tgacagtccg cacgaaagga atgacctgtt ttcgatcatc atagactttg
    25321 gatgctattt ccttcttaaa ggctggttca tcgcattcgg caagtagagc tagggcacct
    25381 tgcttcagcg tagcaatcat tgtttcgtat gcatgcgatt gtgttttctc ccatgatgcc
    25441 agcgagaact ccctgtcgct atcgattgca tcaaggagtc tgccataaat gaactgtcgt
    25501 ggatcgtctg cgtttgtcgc atgcttaaag tcgactttca tccagagaat cttattatcg
    25561 attatgctcg ctgcggtgac ctggcgcgta tattctaaga atgtgctctt ccctgcgcct
    25621 acaggaccaa ggagaagaat gaactgaaga ggtcgctcga tatgggtcgt ttgaatgagt
    25681 gaatctaggt gtgtttgctt ggacttcttg cgtctcggcc gaatcactcg tctttcaagg
    25741 tcgggcttca cgtccatgaa ataagttcga agtcgtgaat cgtatttcag ccgctctgag
    25801 gttttcacgt aacagaatcg taggccatcc acgtccctta agagagcttc gtctgtcatc
    25861 gctatggcga ctgtcggttc gatgtgttca tagacggcat ttcgaccgat tcgaaatccc
    25921 ggctccttca cgacggtcaa gagacgcctc cgtaccatat catcagtaga tccaaacaaa
    25981 gttgtttcaa gcccaccctc aatcacccgt tctcgcgaca gaagattcca gaaatcaata
    26041 aaacgcgcat caatgtccgc gaagtcctga aagacatgag cttgcgtctt ctcgtacggc
    26101 acgccgtcgg ttctgactgc cggaaacaca atccagcatg atccgtttgt agcaacggca
    26161 aacggaatat tcttcttccg gcaatagtcg cgcacttgag cacacgcctt tccagcagct
    26221 gtgccggcta ccgcaccgcc gaggagacat gttcgccggt taggtggaat attaaacgtg
    26281 gtgccttctc gcttcgcctc tagaatgaat gcggttgccg gtgttcgtac gatgtagtcc
    26341 gagtacctag tattgttgtc ctccgtgacg cgctcctcgt atgaaatatc agcatctgga
    26401 ttccatccaa gaacggactc caaaatcgta tctatgacac gctttcgcgt tgttgcttca
    26461 tttgccgcgg aaatgtcccg tacttctagg gattcgacga gtttacggaa tctctgaatg
    26521 gctttatcga tcatattgtg gttccgtttt tttcgcccaa cgtcagtgat gaggctgagg
    26581 cgacttgccg ctgagaagct caaacgcctt atttaactcg ttcattttgc gtgaatgggg
    26641 cgtttaagtc cagccagaat cattcatcaa tagagatgaa aatatggtgg tctatttccg
    26701 gatgcagtgt gaagaaaggg aattctggac tttcgccttt gttgccctga ggcgcgacga
    26761 ttccgactca tctgcggggt ttgggcgaga ctcgtagtat gccaatatgg cttcgcccta
    26821 tccgtcttgc atctaggccg agcttatcgt gtgaatgatc ccggcaaaaa attggcattt
    26881 aggcgttgaa aagacgtgtt ttgaggctca gttcagagtt caactaaagt gatgtcttgt
    26941 gttacccaga gtgtttacaa aagatccgtc ttataagggt ctaaaagtac accctttcgc
    27001 ttttttcaac gttggggttt acttgtgatc aattgtcgca tggcgacaga agctatgaga
    27061 ctatagcaca atgccccttg cctttaaagg gtctgcgtag cagattccac ggtaatcgcc
    27121 aaggtggctc gttgccaact aaactgtgtc ttcataaagt caaaatcagg tccggacgtt
    27181 ataacggcgg cctttccctt gatcaagaaa ccggtacccg gcccatgcct tcccggaact
    27241 tcgcggcttc ccaaagtcat cagcacatcg ggattgccgg caacgttggc ttctgtctgc
    27301 tgcatcctcc cggcaggaac gagcaagcgt ccgtctgatg atattcgaag atagctattc
    27361 caagtgttga ccatgtgcgg tccatccttt cccatggtcg caatagcaac aacaccctcg
    27421 tgtttcataa tttccaatag tttttctggg atcatcgcga ccacctccta tagtttagtt
    27481 cgatattttg cctcacgcgc caccacgcat ccaccgcact tgaacggcgc aatttgaagg
    27541 aggtggttct tccctcggtc atttccattg ccgaagttcg ataataagtc catccggatc
    27601 ccgtagctcc cctcgatagg aattcccaag gtcaaccgga gctagagaca tgcttatccc
    27661 gcgggtcttc agatactcga cggcctttgc catatcctca acttcaaggg caatagccct
    27721 gagtcctatt tgccacgggg actcggctcc cggcttggga tcatcaacgg acatgacctc
    27781 gatggtcgtg tcgcccagtc gaagatagat aacctctttc agcgggggtg cgttcaccgc
    27841 ttctctgcta atcacctgaa agcccaagat gtcggtgtag aagcgaatgg tacgttccgc
    27901 attgcgcggt atgatctcca catggtcaat cctcttgaac ataacgcgcc cccttcgaac
    27961 tccagtattc cttcttgtat ccctctgtgg cctgccgaac gtcaccgccg ctcgtctcca
    28021 gcacctcgtt tacctcattc attctacgtg gcctacgagt ttgagtccag aaaatgggcg
    28081 ttttgacatc gaaaatggct tgtttcccga gtttggaggg catttttggg tgattttggc
    28141 gattttgggg cgcaaaaccg gcacttttcc tcattttttg gcgtaacgtc accccccccg
    28201 ctaaaacgtt gaggtttttg gggataactc cttatttttt gaacgtcagt ggttacgttt
    28261 cggaacagag cgacgataac gcgccggcga ttgttcagca tggctcttga actcgtatag
    28321 tttgatcggc aggcacttct tgtactgtcc gaaatccaga tccgtcgtga atatcgctga
    28381 atcactacga accgcaacgg cacagatcag aaagtccgtg ttggaccctt gtattccttt
    28441 ggcgcgacag gcattgaaga atttcgcggc caatacatag tctgcggtca ccaacgacaa
    28501 atcaggaaag gcggcaagac tggattccaa tcgcacaaat tgcgtttgat cccgcacgcc
    28561 ggagagcagt tcttgccgaa tcggcccaat gatctcaacc gcccgggccg cgatcagatt
    28621 cttgagttct tccgcctcag gatgacgcac ttgcgcttcg cggcgcaacg ccagggacca
    28681 gacgctggtg tcaaccagaa ccctcatggc cgcgcccgtt gctttttgta atcataatct
    28741 gggtcgaact cgatggtccc gaatacgttt aggatgcgct tctggcgcaa gtgttgaata
    28801 tagtcggtca atgcctgggt taccgccgcc ttctttgtgc ggtgcttccc gagggaaacc
    28861 gcctcacgga tcagccgatc gtctagttgt aggtttgttg ccatgctgac cacctccaca
    28921 cataatgtca cacatggcag tgtgtaatgt caatccgact tggacaatat atttttcgtc
    28981 gccggatgcc cgtccttatg gctgaagcta tgggaaaggg cagatacatc gtgcccccct
    29041 cctatcgggg ttttagttga tgctttaagt tgcgcacaag tggaaagatc gaaacctgcg
    29101 cggatagcaa aaagccagag ggaatagtgg ggttttatac actcaaagta cgggataaac
    29161 agtccgccgg ggggcacccc agccgaccaa aatgccgcgc gggcgctgcg cacaacggct
    29221 tggtgcttag tgcgttattt ttgtggagat tggttacgcc ggaggcggtg cgctgagggc
    29281 ggcagccagt tgggccagcg ccacgttttg ttgttcgccg ctggtcatgt ttttgactgc
    29341 ggccacgccg gcggccagtt cgtcatcgcc tcggacgacc gcccacgcgg cgccggcctt
    29401 gttggccgcg cgcatctgcg atttcaaact gcccgcgggc gccatccggc acacgaggcc
    29461 ggccgcgcgc agttgctgca tcaggcccaa attctgctgc atggcggcat cgcccagccc
    29521 caccagccac accagcggcg gcggcgcggt caacagcgcg gcatcacggc ccagcgcttg
    29581 cagcacaccc accactcgtt ccaaaccgat ggcgaaaccc acgccggtta gttcgcgccc
    29641 gcccagttgc aggcggtaac gcccgccgcc ggccacggcg ttttgcgcgc cgagcgcggt
    29701 actggcaatt tcccacacgg tgtgctcata atagtccaac ccgcgcacca gcagcgggtc
    29761 caaatcatgc gccacatcca gcgcggccag gtgctgcgcc acttgtgcca aatattctcg
    29821 ggaagcttcg ctcatgaacg cagtcggcgg cggcacttgg gccacgatgc gctggcacgc
    29881 cgcgtttttg cagtccagca cgcgcagcac attttcgcct atgcgccgtc ggcaatcatc
    29941 gcacagttcc gcttcatggg cggccagcgc cgcgcgcaaa ccggccgcca ctttcgcgtg
    30001 accgcccgct tcgccgcgcg tattcaaccg cacccggcaa tcaccgatgc cccaggcccg
    30061 cagcaaatcc acttgcagcg cgaggatctc ggcatccact agcggcgacg gtggttcgag
    30121 gcactccacg cccaactggt gaaactgccg ccgccgaccc gcttgcggac gctcgctgcg
    30181 gaacattggc cccagataaa accagcgcgc gcccgcggtt tcctgctcgc ccaaaccggc
    30241 ggcgtgacgc atcacccccg ccgtgccctc cggccgcaaa cagagccgcc gcccgccttt
    30301 ctcaaaaaca tacatttcct tttgcaccac gtcggtcgtg gcgcctaggg agcgggtgaa
    30361 gaccgcttca aactcaacga tcggggtgcg cagctcttca aagccatagc gggcgagcac
    30421 cgcgcggccc aattcttcca agcgctgcca cagccgcact tccggcgcgg caatatcggc
    30481 cattccctga acgggttgag tggttgaaga catgcaaact ccctgcggac gccgcgcgcg
    30541 gcgttggcgc tgcttcgatt tcgcgctgga ttccggtgcc tacttgaaca gcgctttcat
    30601 gtccttgccg ggacggaagc gcaccacctt gcggctggga atcaccacca cctgatccgg
    30661 gcggtgggga ttgcgcccga tccgctgccg gcgctcgcgc acatcgaaca ccccgaacct
    30721 gcggaactcc accgtttggc cttcgcgcag ggcatccagc agcgaatcca aggtcttttg
    30781 aataacggct tgcacctgtt gttgcgtcaa gccggtttcc tcggcgatcc ggatcgccaa
    30841 gttgcgttta gtcagatttt gcttctcatt ggccatgtca ccctcgcctt catggacgtt
    30901 tccacccaga accctatcca cggttggaac atacggtcca ttgggcagtt acgtcaagag
    30961 aatcctctcc caaaaatccg gccggcgccc cctgtttttg ctccccggtt tttcgtagga
    31021 tagttttgtc tcttcccccc aaccgtcgcc tgccccccca ctccgatccg atgaattgac
    31081 ggtaagcagc tggatagagt atccacaata ttctacgaag aaccaaaata atcaataatt
    31141 gccagataaa aacgcttgat ttcacttata attataagtg aaattaaagg cgtaaattat
    31201 atcgcaacct atttagatac aattgattat gtactatttg tagaacttta tggtttcttc
    31261 cccaagacaa acgccggccc tcctctccat tctgacgggc tgtgggtggc ccattgtcgc
    31321 gctgcggatg ggtagcctga tgacggccac gcccaatgta gtagacaaaa cggctacaga
    31381 attgacaaaa atgctctagg ccagttggcg atagaccgcc cacgtctggc gcgcggtttc
    31441 ttcccacgtg aatttggccg cctgcgccaa tcccgccgcg cgcaggcgat cggcccgcgc
    31501 cggttccgct agcaccccgc ggacagcggc ggcccattct tcaacgtcga agccatccac
    31561 ccagacgggc gcatcgccca gcacttccgg cagcgagctg ttacgtgccg cgatgaccgg
    31621 cgtgcctcgg ctcatggctt ccagcggcgg aaagccgaac ccctcgtagc gcgtgggata
    31681 cacaaacgcc gcggcgccgg catacagcgc ggacaactgg gcatcgttca tgtgcttcac
    31741 cagcttgatt tgctcgcgcc gcggcgaata cgtcatggcg gacagcgtgg gccccgtctt
    31801 ccagcccagc ccgccggcca ccaccagctc gccgtcgaaa tcagttagcg cctcaaagat
    31861 cttgatcagg aacgggatgt ttttacgcgg ctcaatcgtc cccaccatca atagatacgg
    31921 ccggcgaata ccgatggctt cccgcgcggc ctcagcggcc tccggcgcga gtggcggccc
    31981 gaacttcggc agccccagcc aaatcgggaa cactttttcc gcgggcacgc gcagcaactc
    32041 cacaatttca cgcgccgaga aatggctatc ggtcagaatg gcgtcggcgc gttcggccgt
    32101 gcggtggatt tccgccgaga gccacgccaa gttgcgcgcc tcggtggttt ccggcatccg
    32161 caaaaacgac acatcgtgaa ttgtgaccac cgatttttta ccgcgtccca gcggcgggcg
    32221 gataaagttg gggaagtggt acacatctgc cgctcctgcc aaccattcgt agggcggcca
    32281 gccgaggcgc ttccagaagt tttgcgccac gcgccccggc agccagcggc acgcccgcaa
    32341 cgccaccgcc ggtggcaatt ccggcaaacc ctgccgcttg aagtcgaaga agaaggccga
    32401 cacttcatct gtccccgcca gcggccccaa gtgccacaac agctcgcgga catagcgccc
    32461 cacacctgcc cgctgcccca gcgccgcctg aatgtccatg cacactttca tgagcgcacg
    32521 ttgtagcatt ccctgtcgga aagcgaaagc cgcctttcct ttccgcacgc gttagcgcgc
    32581 tgcgccgccg cgttttccga cgttgtcttt ttgtcgccgg ttttgcattc tggagccatg
    32641 tcggaattca agctcacccc ggaagaaccg ccgccgcccg aacgtaaaaa aattcggcta
    32701 acgaaagctg aagcgtacgc gctgagcatg gccagcccca agaaaaagcg cattgcgttc
    32761 gcattggtgg ggctgctgat tgccgccgcg ctgggcaacg tgctctggta ttttcaagac
    32821 cactggctgc cgttctggtt cccggaaaaa ccggcggccg cgcctgccgt ggagcccgcg
    32881 cccactgctg cgccagccat atccgacacc gcgcccaccg ccgaatcagt ggcgcccatc
    32941 accgatccca ctgcactgga ttttctttcc gccacggtct gggatgatcc gcaattctta
    33001 caaggggtcc ggctattcaa ccaaacgctc gaccgctatc gcaccgaaac aagtagcccg
    33061 ctgccgccgg tcggactgga acaaatcgag cacaatgccc tccaagccgc cgcgcaattt
    33121 tccgcgttgc gcgagcaggc cccagcaacg gtgccgctgg ccgactacat cgcccgctgc
    33181 cagcagttgg cgctggccgc gcgccgcgcg cgcaacccgg aagctaccgc caccctcccc
    33241 cccccgcccc ctcctccccc cgcccgcccg accgcgccac ccgagcccgc catgccactc
    33301 aagcccggcg aaacttggca ggatccggaa tatttgctgg gcgccaattt gttcaaccgg
    33361 gccctggcgc aataccgtgc attcctgaac aacaaagcgc agacgcacct gctcaaggaa
    33421 atcgaggaca acgcatttca ggccgccaag acatttgaag ccatcaagcc gcgagcaccg
    33481 gagggagtgc ccattgcgga tcacatcgcg cagtgctatc gcctgatttc cgactgccgg
    33541 cggcagaatt tggagcgcgg tgccgacgca ccggccaccg gcggtccaca aggaaacgtg
    33601 gtcggccccc gccaccgtcc cgcactgccg gcctaccaag cgccttgatg cgccgtgccg
    33661 cgcgcgccgg cgagtaggtt aaatgtgcgt gccggccgga atgacggcct tcttcgggat
    33721 gacgatcacc ccgtcttgca cggtgtattg atcggtcacg acgttgtcct tgccgtcggg
    33781 cgtgatgtaa cagccatcac cgatgcacgc atttttatca atgatggcgt tgcggatgaa
    33841 gcagtcgcgt ccgacgccca ttgggatttg gcctggcggc aggggttcgg gatcgtacca
    33901 gtccgcgccc atcagcacgg tatgctccag cacgctgccg gtgcccacct tggcgcgcac
    33961 gccaatgatg gaacgcaaaa tgcggtgacc ggtgatgatg cagccttcgc tcagtagcgc
    34021 gcggttcaaa tcgcaggcgt taatcttgga cggcggcagg tagcgcatgt gggtgtacac
    34081 cggcgcattc acatcataga ggttaaactg cggcaccatc tcggccaact ccatgttggc
    34141 ctcccagaag gcgcggatgg ttccgatatc gcgccagtag ccctcgaaca gatagcggta
    34201 caccgcgcgg tccttgatgg cggcgggaat gatgtttttg ccgaaatctt tttcctcgtt
    34261 ggccaacagc tccagcagca cgcgggtgtt gaacacgtag atgcccatgc tggcgagata
    34321 gcgctcgggg ccgttgccgg gttcgcgcag ttcgtccagc aggggtgtgt cgcccggctt
    34381 ttccacaaag cgcacgatgc ggtccttttc atccgcctgc atgatcccca gcgcgcccgc
    34441 ttccgcgcgt ggcaccggct tggtgcaaat ggtcacttcg gctttgttgc gaatgtgtgc
    34501 ttccattacg gggcgaaaat ccatctggta caactggtca ccggaaagaa tgaggatgta
    34561 atccggttcg cggtccatga tgtagcggat accctgccgc acggcatcgg cggtgccttg
    34621 gaaccaactc tcgctgctgg gagtctgctg cgcagccaag atgcgcacga agccgctgtg
    34681 gaactggtcg aacacatacg tgccctgcac gtgttgatgc agcgagacgc tattgaattg
    34741 cgtcaatacg taaatgcggc ggaaaccact gttgatgcaa ttgctgacgg gaatatccac
    34801 gaggcggtac ttgccggcca gcggcacggc cggtttgcac cgatcacgcg tcaagggttg
    34861 caaccgcttg ccttcgccac cgcccaaaat gaccgccacc acattcatcc ctgcacctca
    34921 tcttcagttg ggccgactgg ccaactcttc gccggataat atctggccga caactggtaa
    34981 aatcaatcaa aaataccgcc ctgttttacc gcgttttacc tcgccccccc cgcccgccgc
    35041 ggcggaaagg tttcgtccct taaccattcc cgccgattcc ggacaatggg cgcgggaatg
    35101 ccccgaatca gtccggaacc ggcaccgttg cgccggggaa tacggcggct gccgccgcgc
    35161 ccagcgcggc cagttcagca tcagtgcgct tgggcttgtg atggatcagc cacaggcgct
    35221 tggcggcaca gcgttgcgcc accgccacgg catcagccca cgtgctgtgg ccccagccgc
    35281 gatgggccgg atattcttcc ggcaggtact gggcgtcgaa caccagcaga tcggcaccgt
    35341 gagcaaattt cacaaacgac tcctgcgccg ccggagccat cgccggccat tcgcaatctg
    35401 tggccaccac catgctctcg ccggtggcgg gctcatccac gcggtacgcc acacatccat
    35461 ccggatgcgc cacctcgtgc cagcggatga ctaatccgcc gacggcagcc tgcatggccg
    35521 gcacggagta gtccgcggtc gacagcgtca cgggccatat cggcggcgaa aaaacccgcg
    35581 cgagggtggc agccaaatca ccgcgcggca gcacgagctg acggggccac ttattttcca
    35641 gcaatggcaa acccagcagg tggtccaagt gcgtgtgcgt aaaatagagc acgtaatccg
    35701 cgcgcagccg tgataacaat cgccgcacgc cgctgccggc atccagcagc agttgcgccc
    35761 catcctgccc ctccaccagc atggcgaacg tgtcgccacc atggcgcccg tattccggcc
    35821 ccgataccgc aaaacttccg cgcgcaccgc ccagccaaat cttcatgcca tcctcatttt
    35881 tcagccaggt tccagatacc gtcccacgtg gcggcgggat ggttggccag ctcgcggcac
    35941 ttggcggtat agacctgcgc ggcggggtcg gcggacaacg cggcaaagcc tgccgccgcc
    36001 gccacccagt tgccggcacg caccaaggcg agcgcatcgg caaacgcgtg ggtttcgggc
    36061 gcgggcggtt caccggcaaa acccgtggcc tcaaaaaccc gcaccggggt tttgcgtccc
    36121 accaccgtca actgcgccaa ctcgcgaccg aggaacgcgc cttgcgtttg ctcccaagta
    36181 ctttcggcaa tcatcaaatt ggtgccgaag gccttgttgg cgccttccag ccgcgaggcc
    36241 agattggcgg catcgcccag catggtgtaa tcaaagcgct ggctggaacc catgttgccc
    36301 accaccaccg cgccggtgtt gagcccgatg cgcattttga gcggcccgcc gatgcgttgc
    36361 gcgtaggccg cctgctgctc ggccaacacg cgctggcaac gcagcgccgc gcgcaccgca
    36421 cgcacagcat ggtccggttg cgccagcggc gcgttccaga aggccacgat ggcatcgccc
    36481 acatatttgt ccagcgtgcc gccttccgcc aaaataattt cggtcatggc cgacaaataa
    36541 tcattcagca ggcgcgtcaa ctcttccgga ctgaggcgtt cggaaatcgc ggagaagccc
    36601 tgcaaatcgg aaaagaaaat cgtcagttcg cgccgctcgc cacccagctt gaggctgttg
    36661 gggtcacgca tgatttgctc aatgacttcc gcgctcaagt agtgcttgaa agcgcccttg
    36721 ataaacattt tttggcggcc ttcaaggatg aagttcacca ccgcgccgcc caagagcgcg
    36781 aggatcacac tcgtcagcgc gcccgccacc ggcagtaccc agttggcttg ccacgccgcc
    36841 aggccggcca gcaccggcac cagaatcagc gccaccgcca agacggcgct ttgccaaccg
    36901 ttgcgcgccg cagccgcgcc aaggccggcc agtagcgcca gcagcagcgt ccatccccac
    36961 gcagccggcg gcggcacgtc gcgcaggaaa tcgccgatca gcagattgtc cagcatggtg
    37021 gcatggattt ccggcccggg ataatccgcc gcgaccggcg tgggccgcaa atccttgagc
    37081 ccgggcgcgc tgaatccaaa caccacaaac ttatccttga acacactgcc atctgcgatg
    37141 ggcggggtgc caccctcctg caagcgcagc tcggattgaa tcaccgccgc cgccgaatag
    37201 gcggggtgcg tgccggacgg cccgcggaat ttcaaaatca tccgcccgga atcatccaac
    37261 ggaatctgcc gcccggccag cgtcagcgta ttcccggtca gttggccggc aaccggcgtg
    37321 ggttgcccgg cgagccagca cgccacgccc aacgaaggca gcacctgatc tgcaaaaaag
    37381 cgcagcggca ccgcccggcg gaaaatgccg tcgctgtccg gcacttcgcg cacgttggcc
    37441 agccacgcgg tattcgtcgt cagttcggga accgggaacg cggcggcggc ggaaaccatg
    37501 tccgcccgtg cggcgggttt tgctgccagc caatccgccc accctgccag atgaagcgga
    37561 ttcggctgag ctatctccgg ccacgcatcg gtttgcgtgg tggtggtgcc cagaaaaacc
    37621 gccgcgacaa agttcccggc gcgcccagcg gccgcgccga gggcttcgtc atcttccacg
    37681 ccgtaggtgg aaggctcggt gtagagcaca tcaaaggcca ccgcgcgtgc ctgattgcgc
    37741 gtcagataat ccagcaccgg cgcataaatt tcgcgcggcc aaggccacga aagcccgatg
    37801 ttctggctgg cccaatccaa actggcctgg tccaccagaa tcagcgcgat atcgtcggtg
    37861 gccgctgagg gtttggccag caggcgcgcg cgccaatccg ccaacggatt ttcccagcgc
    37921 gcgagcagtc cgccccactg gagcaagcct gccacggccg tggccagcac gccggccagc
    37981 aggccgtacc ggagtttttt gttcatcggc ccggcgctcc cggcttactg gccttgcgtc
    38041 gccgccaaga agcgctgctg gcgggccggc gccgaggcct tggtggatgc cacgcccagc
    38101 ttttgcaatt gggcaatgcg atccgcgggc gcggggtggg tcgccgcaaa atcgtgccgc
    38161 gaggaatccc aatttttcgc catattttcc agcatggcca ccagcgcgcc ggactggtag
    38221 cccgcccgcc ggagaatacc caccgccgca gcatcagcct catattccaa tttgcgcgaa
    38281 tagccgctgt tcatcagcgt ggtggtgatg tcgttgatgg attcgtcaaa cgccttggtc
    38341 acttccgcca actgctcgcc gcccagattt ttgccggcct ccaccgccaa aatggtcagc
    38401 gccgaattga gacggccggt gcggatggca cgcaacccgt gctgcttttc cacgtggccg
    38461 atttcgtgcg ccaacaccgc cgccaactcg tcttcggttt ggcagcagtc caacaacccg
    38521 cgtgtcacca gaatcaagcc gcccggcgcg gcaaaagcgt taatctccgc cgagtccagc
    38581 gccagaaaat gatagccacc aaatgtttcg ggccgctcgg aaaattgcgc cagcgattgc
    38641 cccacttggt tgaggtaggc attcagcttt tccgccggca gcggtttatg ctgggtcaat
    38701 acagtcgccg cgaccgtgcg gccaatgtag tactcctgtt ccggtgtgat gtcctggaac
    38761 gtcttggtga cggctgtgct tacccgcttg gtgctctcgg cctgctccgg cgtaatcgcg
    38821 ccggtggcgg cggccacatc ggcggccgcg gcggtaatct gattgacggt ctcgcaaccc
    38881 gcggccagca ccaagctgcc gaccaccaac gcgctgccca attttttgca aaatgcggat
    38941 ttcatcggtc gctccttact gggccaggcc gccctgtttc aggaattgca ccaactgctc
    39001 cgcggtgagg gtctgttggc ccatccaatc cacccacgta tagtcgagct cccgattctg
    39061 ttttttgtat tccgcctcaa cttccttgct gaagcccttg ccggccagcg cgacttccgc
    39121 gccgctggcg cccacttgcg cgtctgacgc gcccgacgcc aacaccacgc gcttgggggt
    39181 caacgccgaa ctatgaatcc agcctcgcgc gccggcggcc gtggtgactt cgcaccagcc
    39241 tgcctgttcc gcgcaaaccg tcacacggtc gccataggcc acgttggcca ccaccgcgcc
    39301 caaaaacgac gcgcggttcc gtaactggcc gtcgcgcacc tgcacgctca tttccttggc
    39361 cgcccacgcg ctggcgccaa ccagcagcag actcgccatc aaccaccatt tcttcatgtc
    39421 catcctccac ccattactta ttcagtcgtt gctcagcctg aaatgttaca tatagcgccg
    39481 ccaatttttt cgccaacggc gaatccggca tttgctccgc aatggccagt ccctcttgca
    39541 gccgggccac ggcctccggt tgcacgccct gcgcccagag gctgcgcgcc gcgcgataca
    39601 gccgctcgcc cgcgccggcg gcatcgccgg ccgcttgcgc ctgctgcgcc ccggcggcca
    39661 gcgcacgcgc catctccggc aagcgatcgg cttgctgcca gaggcgcgca gcttcatccc
    39721 aatgtgtcac ggcagccgcc gggtcgccgc ccaccgcggc aatactcgct tgctgtcccg
    39781 cgcgagccgc gcgcaacgat gccggcacgc gttgccattc attgggcttc gggccatccg
    39841 ccagcgcctt cgcggcggcg gcggcatcgc cctgcgccaa ctggatttcg ctatgcagca
    39901 aggcagcttg cgcgcgcagc agcgctgggg gcgtcatcgc ttgcagctca gccaaccgcg
    39961 ccaatgccgc gggggcgtca ccaacgcccc agtcggcgcg cgcaccggca gccagcaatt
    40021 ccatgcgccg ctcgcggctc acgcgcgggt cggccaaggc ttcctcgatg gccgtccgcg
    40081 cttcgccggg ctggtcttcc gccaagaggc attgcgcgcg attgaccgcc gccaccgcca
    40141 aggcgttggc atcatccatg gcccgtgcgc ggacttgcgc ccgctcgaag gcactggcgc
    40201 cgcgccgata atcgccgcgc gcgtagagac cgcgcccggt ggtcacgtgc gacacccaat
    40261 cttcatccgt cacttgcggc gccggcggcg tggcgcaccc cgccaacagc atgaccgcca
    40321 gcacagccac ccccctaact atgaaccctg aaccctgacc tctgacctct gacctctgaa
    40381 ccctgaaccc tgaaccctgc cccctgacct ctgacctctc tcttctcatg gcaccaacac
    40441 ctcctgggcg cgcagggcat catccgagcc catatatttc cgcagcagcc aatgtttttg
    40501 caggccatcg agcagcgcgg tggtttcccg catcagcgtt tgggcgtcgc cggtcagcac
    40561 gggcaggttg tccacctcgt cggcgacagt cccgctcacc ggcgcgatgg tgctcaccat
    40621 ggtttgcagc tcggcggcga tggcgttgat cttgtccatc agctcttgaa tctgcgccac
    40681 gatgttttcc acatccttgg ccatgcccgg atcgttcagg attttggcgg gcaggcccgg
    40741 gcctttgcgc aggtcatcca gcaggccgtt cgagttggcc agcaactgtt gcaggctgcc
    40801 atcgggatcg ttcatccccg cggccaaatc cgtatattcc gcgatggcgc cattcaccag
    40861 cgtgagcgtg ttcaccgctt ccttgcggat ggtctccaac gcctcctgca gcatctccag
    40921 gatttcggta tccttcacgc attcaatttc gccgccggtc cgcggcagct ccgcgccagt
    40981 gccgcgggtc agctcgatat aggcatcgcc ggtgacgacc attttctttt tgaccagggc
    41041 aatggagtct gcgcgcacga agcgcatgaa gggcccgcgg atgcgcatca cgccggaaat
    41101 gtggccgtca tcgttcacga caatgtcctg cacgctgccg acttttgcgc ccagcagcat
    41161 gacctccgcg cccttctgca ggtccatgga gccttcggcg gggaagttga ggttgatcat
    41221 gtatttcgcc tcgaaccaat gctgcgcgtg gccggccacc agcacccctg ccagcagcag
    41281 caacaccacc agcagcacga agccgcccac gatttcattc acatatctga atttgaatgg
    41341 ttttgccatg ttcgctcctc atgccggttc cggcgtgatc accgtcagcg gcgccagcgc
    41401 cgcgcggaca tcttctgcca aggtttcttc gagccaaatc accgcgcaac ccgcggcccg
    41461 gacatcggcc accgccgcca gaaacaggcg gcgatcctct tccggcatgt tgcgcagggg
    41521 gcgctccagc accagcgcca gcggccggcc ggcgaacgcg cgcgtccaga gcaggcgctg
    41581 ccgctcgcgc tcgcgcacca cggccgggcg acgggcgggc aacggccagc agcggaaacg
    41641 ccgcgcccag gtttcgatct cggcggcggc gccggtccgc cggtgaatcc gcgccggcag
    41701 ccagacgttt tcgtccatgt ccagattcgc cagcgcgccg ccggtggtca acacgcagcc
    41761 gatcgtgccg cgcgcggcgc tcgccgcatc tggcgaatac gcctgccagt cgcgcccctg
    41821 ccaatgcacc gcgccttccg gtggcgtggt cagccccagc agccaatcca gccaatccag
    41881 gccgtcgtca tcactatccg gaatccacgc cgcggcgcct gccgccacgg aaaaattcca
    41941 caccgggcct tgccgcgcgc ggactcccgc cacccgccac aattcgctgt tggggttgcc
    42001 agtcatcact gcctcacaca aacgccataa tggaaatcaa cagcgacgtc acaaacaacg
    42061 tcgccaccga tcgaattacg ccacgcgtgg ccgcaatcgg cacttcggtg gcggcgacgc
    42121 ccactgccag gccttcatcg cagcaaatcg cgccggtcag cagccctggc acgatggact
    42181 tcgccagcag gctgaacaca tccggcaccg tcaccgagcc aatgatgttg ccgaagaaca
    42241 ggcccatgtt cacatcgccc agctcgatgg cccaaatgca ggcgaagccg ccgatgaacg
    42301 tcaccgccaa aaagatgatg gtcagacaaa acgtggaaat cgccaccccc agcacgcgcg
    42361 gcaccaccag atacacaaac ggatcaatgc ccatggcatc caacgcgcgc acttcgccgt
    42421 gcaccttcat gttggccagc tcggtggcga tcgccgtgcc gctgcgggca atgaccacaa
    42481 agttggtcaa cagcggcccc agctcccgca gcaccaccgc cgtcagaatg ggtccgatca
    42541 aattcgactg ccccagccgg gtcagccagt attgcgcctg caccaccacc aacgcgccca
    42601 cgattaccgc gatcagcgcg ataaaccccg tggcctcaaa accggtgaac aacacctgcc
    42661 gcgccaagac gttgcgcaaa ggcgcggtcc aagtgcgggg gcggattgcc cgcagcagga
    42721 cggcccacgt aatggccgcc agacgcgtca ggcggctcca ccgcgtcaag acgccggagc
    42781 cgatgtatcc cagaaagctc attcggccgt cagcatagcc cattcgacag gcagccgaca
    42841 atcccctaac ccgcccaatg ccggttcctc gctgttttca gtggccggat cgtctcgctt
    42901 gggatgcgga agcaaccgct gtcagaccac cttctatttt tcaatttgaa ccatgctgtc
    42961 cagagtttat aaactgtgga caaatgtcat aatatttata tctcacttat atacaatttg
    43021 ttatgttcat tttttgggtt atttttaatc ggctgttttg agggtgatgt ccacacttta
    43081 taaactctgg acaaatttaa ggtgactgtt ttttgcggtt ttatccgtgc ggtgggaact
    43141 cgaatggcag ttgccttccc cgatgccatg cgcgccattg tggttgcgct tacgttgatc
    43201 tcaggtgcaa tccgggaatt cgcgccagat gctccgccgt gatgggaccg gggcgcagga
    43261 tttccgcctc cgcgcccgcc acgcggagca ccgtgctggg ttggccgccg gtgacggggc
    43321 caccatccag caccatggac aaatgcggcg acagggctgc ccacgccgct gccgcggtgc
    43381 ccgccggggc cttgccgctg cggttggcac tggtgacggc gggaatcaca ccgcccagtt
    43441 cgcgcagcca agcttgcgcc acatcgtgag ccggcgcgcg aaaccccagc cacgcgccat
    43501 ccgcgccggg caagaccagc gtgagcgggc cgggccaaaa cgcaacggcc aactgccggg
    43561 caatatcgct caccgtggcc ccggccgtgc gcacgccgtc caggtccgca acaaaccgtg
    43621 ccacctgttt gctttcgtcg cgccccttgg cgcggaacag tcgggcgatg gccgcgggat
    43681 cgtcggcgcg ggcggccacg ccatacacgg tttccgtcgg caggcccacc agttcgccgg
    43741 cgcgcaacgc ggcggcggca tggcggatat tggcgggcgt ggccgggaga atgccgaccg
    43801 atctagtgtt ggcggccata gccgagcgct ttcaggccca gcagtacatc cgtggcgcgg
    43861 cgcaaatagg catcgtgccg ggtgcggtcc cgtagcgctc ggtcttcttt ctcttcttcg
    43921 gaaagcgtct tgcccttgcg cagcacgggg gattcgggtt cgtagaatgt ctcgtgcaac
    43981 gccgcggagc tgatcaccac atccggctgc acgccgcgcc cgccggcata gaccgcgccg
    44041 ttggccgctt tgagctggcg tgtggcgagc agggcatagc gcccggtggg aagttgcttg
    44101 gcttcgcgga tcagcgggtc gccggcagtt tcctgtccga tgaccatggc gccctgcacg
    44161 ctgccgctca ataccgccgc cagtagttcg gccgcgccgc aggtttcgcc atccaccagc
    44221 accattagcg gcagcgttgg ggctgcgggc gccgtggcct tcacggtttt gatctcatgt
    44281 ccctgccggt cgttcatggt gtagagcaca tcgtcgcgcg gcgtgaactt ggccgcgaca
    44341 attgccacct cgtcttccgc ggtgccgtcg gcctcgcgta ggtccagaat tgcgccgatc
    44401 ttgccggccg cctgccattt gtcgaggcag gccacaatct ccgcgcccgc gccggtgaag
    44461 atgcccgccg cccgcatgta gcccatgttc accggtaggt cttcaatatt cgtcaacgcc
    44521 gtgcgctcac gacgggtgcg caccagcgtc aaggcgcggc tttgaccggt cgggtccaga
    44581 atggaaacgg cgagttccgg ctcctcgccc gccagcaaaa ggctgcgcac ctgcttgagt
    44641 gtcgcgccgg tggcaaggtc ctgatcgccg atcttctgga caaattcgcc gggctggatg
    44701 ccggcggtgg cggccggcgt atccgccgcc accgcgacaa tcttcggcag gctgtcttcc
    44761 atttccacca acgtcagccc cagcgtccat tcctgcgtgg cctcctgctg gcagcgcgag
    44821 gccaactcct cctcgtccag aaacaccacg gtcggctcgc tggcgcgcag caacgcctcc
    44881 agcaaagcgc ccttggcggc atcatccggc gccggcaaat gattttcggc cagtaggcgc
    44941 tcgccttccg cgatggtcgc gcacaagccc gccgcatcgg cggcgggctc gaccgccgcg
    45001 ggttcatccg attgggctga cgcatgaacg gccagccaaa gtccggccag gcaaagtgcc
    45061 cgaccttgat tccagagtct attcatgcgt cgccttgcct atccctttta gaagcgaatt
    45121 ccgccgccga ccgagaagat cacatagctg ttctcgatgc cggccacatc ctcttcgccg
    45181 ccgctttcgc cggtggaccc gtccgttaat tccatgtcgc ccacgatccg gctgatcttc
    45241 tggtaatcca gcgccgcggt cagaaagacg ccctgcagcg agccttgggt aaagtcatag
    45301 cgcgcggaca agcccaaccc cagcatgttg ccgccgtcga aggtttcgtg atagtgcaca
    45361 tcgcgcgcca cgtgcacatc ccagtcatcc gccgccacaa tggggctcca agtcagatag
    45421 cccgacagcg taaagttccc ggtcttccaa tccgcgcttg cgcccagata cggcatgcgg
    45481 aactcctgct cataattgat catgttttcg ccgcccagat cctgcgggac ataaccgtat
    45541 tccggataga gcagatactg cccgcggtct ttccactccc agccattctg cttgtagccc
    45601 accatggcgc gcgcaaccaa atcctgggtc agttggaaat cccacgccac attcaggtca
    45661 aaaatgtagc cactttcaat atccacatca gacagcgaat aatgcgtcca gtccgcgcta
    45721 tccacgatca gccaatcata atcctccatt tcgccgctgc ccttcgacag cgccagccac
    45781 accccgccat tgatcgtcag cgcatccatc aggcgcacgg aaccattcac cccgcccatg
    45841 gcgatgccct tcaagtccca atccagccgg ctcaattgcc gccgcccgcc gccgggcccc
    45901 tcgtagtcat agacgtgctc cttggcctcc ccgttcagca cgcccaacga cgcttgaaac
    45961 gccacgggca cacctgcccc atcacggctt cccgcaatcg ccccggcttg aaatgcccag
    46021 gcggacgacg caaaactacc caacaccaac aacgcaacca gttgcctctt catgtgttcc
    46081 cttttgctgt tcaatgctct aatttggccg gtcacccgac cctcaaaacc agattgcacc
    46141 acttcgcccc ccccttggtc aagccgtttg gcgaccattc tccgcccacc cgttgccgtc
    46201 aattgccttt caagcgcagc cccccgattg gactcgtttt caacctcatc cgccgaggga
    46261 tgagtgagcg aggggttctg ggcaaaggtt ttccggctga cagagacaag gaggtccact
    46321 acactgcgga ttcagttaat acgatcaaca acaggaaaat cggatgccgc agctcgaaca
    46381 catcgaagcc atagaaaaac gtttgtggac cgcggcggac acccttcggg cgaattccaa
    46441 ttacgccagc aacgaatact tcatgccggt catgggcctg gtgtttctgc ggtatgccta
    46501 tagccgcttt ttggccatca aagatggcat cgaagcgggt ctgcccaccc gaggaggcaa
    46561 gaagcgggac ctgacgaagg aggatttttc gcagaaaagc gcgattttcc tgcggcccga
    46621 ggcgcagttc gactatctgg tttcgctgag ggacgaagag gaccgcgcca aggccatcat
    46681 tggcgctatg aaatccatcg agaccgatta cgtcagcctg aaaggcgttc tgccgaagga
    46741 ggattatcag gagctggaca atgcggtcct tgggcagttg ctaagaattc tgaacccgga
    46801 ggagctcaag cgggtgtcgg gcgatgtgtt tggccggatt tacgaatatt tcctgaccca
    46861 gttcgccggc caaaaggccc acgacgacgg cgaatttttt acgccggttt ccctggtgtc
    46921 gttcatcgcc cacgtggtgg atcccaagcg cggaatcgtt cttgatccgg cctgcgggtc
    46981 cggcgggatg ttcgtgcaaa gcgcccgcac ggtggaagag cacggccaga gccccgccga
    47041 acgcctgacg ttctacgggc tggaaaagaa cgccaccacc atccgcctgg ccaagatgaa
    47101 tctggctgtc cacgggctgg aaggcaacat ccagaaagcc atcacctact acgaagatcc
    47161 gcaccatctg ttgggcaagg cggacgccgt gatggccaat ccgcccttca acgtggacga
    47221 gattgatgcc gggaagatga aatcggatcc ccgcctgccg ttcggcctgc cggggaccaa
    47281 caagcgcggc aaggtgtcca acggcaacta tgtctggatc agcttcttct acagctatct
    47341 gaaccggcgc ggccgggccg gattcgtcat gtcctcccag gcttccagcg caggcaacga
    47401 ggaagccaag gtgcgtcaga agcttgtcga gactggcgat gtggacgtca tggtcgccat
    47461 ccgctccaat ttcttctaca cccgtaccgt cccctgcgaa ctgtggttcc tggaccgcga
    47521 caaacccgcc gaacaccgcg acaaggtgct aatgattgac gcccggaacg tgttccgcaa
    47581 ggtcacccgc aagatctacg atttcagccc cgagcaggaa aagaatttgc tctccatcgt
    47641 ctggttgtac cggggggaaa ccgacaagta tctggcgctg ctggcggagt actgccgccg
    47701 caccttggaa gaagccgagg cttgctttgc cccgcctacc gatggcgggc aagatgccga
    47761 acctctcccg gaattcatag cggcgatgga ccagctatcc gccgccatga agccgtttct
    47821 gaacacaccg gcagccgagg ctgcgctggc cgcacaaatt tcggagctgg atgcggcccg
    47881 gcaggagctg ccatccgccg ccaatgactt ccggaaggct acagccaacc accgggccgc
    47941 atggaagaaa cctccggcca ccaatgctga gctaaagaaa gtcgttgagc gcctcgcgga
    48001 gctggccgat tccagccgcg acctcgtcca ccaggccgac ctatgctaca agctcgccgg
    48061 ccgtctgatc gcctcctgcg agaaagaatg caaagccaag gagaacgaaa aatgggtgag
    48121 gcgcgatatc aaccatgcca gtaaagccgc taaggaggcc cgggagaccg ccgttgccca
    48181 gctcaagcag gtgcgctatt tctggaaaca agcccattgg ctgacggaac gattccccga
    48241 tgccaagctt cgcgacgtgg cgggtctggt caaattagtg gaccgtgccg aaatcgccgc
    48301 taacgattgg agcctgaccc ccggccgcta tgtcggcgtc gcccccgaag aagcggacgc
    48361 ggatttcgac tttgaagaaa ccatccaaga catccatttg gaactggcgg gacttgatgc
    48421 tgaggccgtg caattggccg cagtcatcca aagaaacttt ttggaattag ggatatgagt
    48481 tggcgaccac aaaaactaga ccagctcgga acgctctccc gtggtcgttc acgacacagg
    48541 ccgcgtgatg ctgctcatct atatggtggt ccgtatccat tcatacaaac tagcgatgtc
    48601 aaacattcgg ggctttacat ccgcgaatac cagcagactt atagtgagga agggcttcgg
    48661 caaagtaaga tatggcccgc cggaacactc tgtataacga ttgcagctaa catagctgac
    48721 actgcgattc ttggaatcga cgcatgtttt ccagatagtg tcattggatt taccgcagat
    48781 gaaaataaat ctgatgtccg cttcataaag tatctgtttg atgcaaccat tcagcagcgc
    48841 gtacggcagt tcacccaagg agccgcacaa gacaatttaa gccaagaaaa attactttcg
    48901 attgagttct tgatcccaga attgcaggag cagcgacgca ttgcctctat tctctccgcc
    48961 tacgacgatc tgatcgccaa caaccggcga cggattcagt tgctggagga agcggcgcgg
    49021 ttgctttacc gggaatggtt cgtgcatttg cgcttccccg gccacgaaca cgccaaaatt
    49081 aaggatggaa tcccggaggg gtggaaaaga ggaccgctgg gacaactctg cgttgaagtg
    49141 agagaaatat gccagccagc atctttggaa ccagatgcac cgtacattgg gttggaacat
    49201 atgccccgac ggtctatcgc cctgtccgaa tggggcacaa cagaacaggt gaccagcagc
    49261 aagcatcgct tccgcgaaaa tgatattctg ttcggcaaaa tccggccgta tttccacaag
    49321 gtcggcattg ccttgaccga aggaattgca tcgtccgacg ccatcgtcat tcggccggcc
    49381 agtcaggagc tattaccgct ggtactgatg gtcacgtcaa gcgacgcatt tgtcgccgat
    49441 actgcccaaa aaatgaagga aggctccaag atgccgaggg cagactggaa gcaaatgacg
    49501 cagtatccag tgctataccc gagcgataat acgctgtggg cgctcaacga cttcattgag
    49561 cccattctgc aacaaatcaa gacgctagtg ttttccaacc gtaatctagc tgtggcccgc
    49621 gatctactgc tgccgaaact gatgagcgga gaggtggcgg tctaatgcca gcgtttgcta
    49681 ccaacggtgc agatattcca gagcgtttgc tgcaagcgca tgaggacggg cgggtcgtat
    49741 ttttctgcgg cgcgggcatc tcctacccag cgggattgcc cggcttttct gggctcgtaa
    49801 aagatctgtt ttcccatctt ggtcaagagc ctacggcggt gcagcaagca gccataaaag
    49861 aaaagcggta cgacacagcc gttgggctct tagaaacaga atatgttggg gggcgagagc
    49921 gggtacggga agccatcaag aagattttga ccgtgaccga aataaaaacg accgcagtgg
    49981 caacccatga ggcgctcctc aggttggcga caaattccga agggaaaatc cggctggtca
    50041 ccaccaactt tgaccggctg tttgaacatg ttattcagca cagcggtcta tccgtggaaa
    50101 cattcgctgc gccgttgttg ccgattccca aaaacacctg gaatggattg gtatatttac
    50161 acggcctgtt gccagactcc aatggcaagc cggatctgaa ccggctggtt gtatccagcg
    50221 gagatttcgg cttggcctat ctggtcgaac gctgggcctc tcgttttgtg agtacgctct
    50281 tccgtaactt tacggtttgt ttcgtaggct acagcatcaa cgatccggtg ctgcgctata
    50341 tgaccgacgc attggcggca gaccggcaac ttggggaaca cccgcccgaa atgtttgctt
    50401 ttggcagcta tcccaaaggg aaacaaattc aggccgagga agagtggcga gccaagaatg
    50461 ttacacccat tctgtatcgg gaatattcca agcacgctta tttgcatcgt acactgaagg
    50521 agtgggccaa gacctatcgc gacggggtac gtggaaagga gatgatcgtt gtccaacacg
    50581 cggcaacgcc ccccttgtct ccgtcgccgt cggattatgt cgccgggcgg atgctctggg
    50641 ccttgaccga tgaacttgcc gcacggcgtt ttgccaacct tgatcccgta ccgccgctcg
    50701 gctggctcga tctgctgacg aatgaacaat tcagccacaa ggacctaccc cgatttggag
    50761 tggcacctaa cgacaagccg gatgaaaaac tgagctttag ctatctccga cggccaaccc
    50821 cctatgtgaa agcaccttgg atgtgcttgg cagacatggg aatgccatcg ccgagcatgg
    50881 atgcggccat gcgcgagatg gcaatctggc tctcacggca tctcaacgac ccgaaggttc
    50941 ttctctggtt ggctaaacag ggtggacgtc cgagtaggcg gtttgtcgac atcattcacg
    51001 atcgtataga agaactggac aggttgattg cgaaaaatga caccgcgcgc atcgcagaga
    51061 ttcgtgcagc cgcaccaaat gccttcccta gtcccgcaat gcgagcatta tggcgtgtat
    51121 tcctcgctgg gcgagttggt tacggaatag accgtctgga gctgcacgat tggttcgatc
    51181 gcttgaaaat aaacggtatc accccctctt tgcggatgga gctgcgcgaa atgctcgagc
    51241 ccaaagtcat gcttaagcct ccttttcgcc tggggctaga ggaggaggaa gaaaaagaag
    51301 agccgacagt gcgtgatctg attgattggg agctgacgct cgccacgggt catgtgcact
    51361 ctttaatcca gcatttgggg catgataatc ccgaatggaa acgagcactg cccgaacttt
    51421 tggatgactt cacgatgctg ttgcgggacg gtatggacct ggcgcgtgag cttggcggtg
    51481 cggaggatgc gcacgacata tcgtatatct accagccgtc gattgaggac aacggccaaa
    51541 accactattt ccagaactgg cccgtgttga ttaaattaac ccgtgacgcc tgggtggaaa
    51601 cggcaaaaac agataccacc cgagcggtac aagtggcaat ggcttggtgg cggctgccct
    51661 atccggtgtt taaacggttg gccatgtatg cagccaccca gggcaatgtt atttctccta
    51721 aattggcttt ggaatggtta ctggatggcg agcgtcagtg gttgtggtcc attgaaacac
    51781 gccgggaaac gattcgcttg ctggttgcat tgtctccgca attgggcgag tccgatctcg
    51841 ctgctctaac cgccgttatt ctgaatggcc cgccacgcag tatgtaccgg gatgatctct
    51901 cttccgagag gtggcaggaa attttggagc atgatatctg gctccgctta gctaagatcc
    51961 ggaaggctgg tggcattttg gggcatgacg cccagacgaa gctggacgaa ctggaaggaa
    52021 aatatcctga atgggaggtc actgatgacg accgcgacga aatgcctttt tggatcagtg
    52081 atggtaagga tttgcgcagt tttacgccga cgccccgtcg gagaatagag ctgattggct
    52141 ggctgaagga caacccagac actgaccact ggaaagagaa tgactggcgt cagcggtgtg
    52201 atactgacta cagcgccgcg atttacgggt tatacgcttt gtccaaggat gatatttggc
    52261 ctgaaggacg ttggagagaa gcgttgcaag tctgggcaga ggataaccag accacgcgat
    52321 cttgggctca tatacactgc ctcctagaac agatgcctga ttcggttgcg cgcaaattag
    52381 atcgtagtat ctgctggtgg ttgcgggccg tggcaaaaac ggcacaaccc gaaaaagaag
    52441 tattcttcag gctatgtaag cgtttcttat cgctgccgcg tgattcggag atgcgggagg
    52501 atattcttgg atgggctatc aaccatccaa taggactact gactgaagct ctgctggatt
    52561 ggtggtataa acaatccccg aaacccgggg cggggttgcc agaaattctg gcgccaatat
    52621 tcacgaaatt atgcaacccc gccaatatca attatcgcgc tggacgcgtt gttttggctg
    52681 cccgtactat ttcgttcttt cgtgtggatg agccttgggc aaggcaaaac cttattcctc
    52741 tctttgattg gggcagatca accgaagagg cccgtatcat gtggcatgga tttatgtggg
    52801 cggggcagct atacagacct tttttggagg ctgttaagca atcatttctg gacgcagcga
    52861 attattatgg agacttaaga gatgcggggc cacgttatgc agagtttttg gctcttgctg
    52921 cggtggaccg cggggatacc tttaccgtag aagaaatgag gcgggcaacc tcacgtttgc
    52981 cgttggctgg gctagtcagt tcactccatg tactagcgcg gtctgcggaa ggtgccggtc
    53041 aacggcaaat ggaatactgg aaaaatcgta ttagacccta tttgcacgat atatggccga
    53101 aatcgagaag atttcttacg gaggagataa aaagagccat ggccgagcta tgtgtagctg
    53161 ccggcgaagc attcccggcc gcatactatg atctcaaaga ctggctttat cctgtccagt
    53221 acccagaatt gatgttgcat agggttaaag cgcaaaagat catcgaaaaa tttccagagg
    53281 aggctctgga ttttatggac aagttatttg ccgatgatcc ggactaccat ggatcgagcc
    53341 tcaaggaatg cctagacgct gttgtcaaaa gaatgccggg ccttgccaat gacggccgcc
    53401 atgaacggct gacattgctg cttcgcaaac gcgggtggct agaataggat gtttccggat
    53461 atgtgcgcta atcactacac cgaagacacg ctggtgcagc agacgacggc ggagtatctg
    53521 gaacagcaac tgggttggga ttcggtgctg gcgtacaatc acgaggactt cggcccggac
    53581 agtttgttgg ggcggctatc cgaccgggag gtggtgctga ctcgaatctt gagggccaag
    53641 ctcgcggagc tgaatcccgg gctgccggag gcggcctacg acgatgcggt tcgccaggta
    53701 acagagacca caacgacgca gtcgttggtg gcgaccaacc gcgaaaagta cgaccaaatc
    53761 cgcgatggcg tccaggtgac ctatcgcgat ggcgagggga atctggcaaa ggagcggctg
    53821 cggcttttcg atttccgcca gccggagaac aaccattttc tgtgtgtccg tgagctgtgg
    53881 gttcggggcg atctgtaccg ccgccgcgcg gacatcgtag gattcgtcaa tggcatcccg
    53941 ctgctgttcg tggagtgcaa gaatatccac cggaacctgc gggcggcgtt cgagcagaat
    54001 ttctccgatt acaaggatac ggttccgcat ctgttccacc acaacgccat cgtgatgttc
    54061 ggcaacggcg accaagcgaa gatcggctcg gtgaccagcc gctgggggca cttccacgaa
    54121 tggaagcggc tggcggagaa tcagccgggc gccgtggata tggaaaccct gctgaagggc
    54181 gtttgcgaca agcggaactt catggacctg gtggagaact tcatcgtgtt cgacgattcg
    54241 gccggcgagc tccggaagat attagcccgc aaccaccagt ttctgggcgt caaccgggcg
    54301 gtcgaggcgg tccgcgatcg caagagtctg gatggccggc tgggggtgtt ctggcatacg
    54361 cagggcgcgg gcaagagcta ttccatggtg atgttcaccc gcaaggtgca ccgcacgctg
    54421 ggcggcaatt tcacgttcct gatgctgacg gaccgcgacg atcttgacac ccagatttac
    54481 aagacattcg ccggctgcgg cgtggtggac aacgaccgcg atccgtgccg ggcggcgagt
    54541 ggtgacgatt tggcccggtt gctgagtctg cacaagtcgt acgttttctc cctgatccac
    54601 aagttcaacc aggacgtggc gccggatgcg gcctatacgc ggcgggacga catcattgtt
    54661 atctccgacg aggcgcaccg gacccagtac ggcaccttgg cgctgaatat gcgcaatgcc
    54721 ctgcccaatg ccagctatat cgggttcacc ggaactccgc tgttcaagga tgacgaaatc
    54781 acgcggcggt ggttcgggga ttacgtttcc acctatgact tccagcgggc ggtggaggac
    54841 aaggccactg tgccgctgta ctacgattcg cgaggcgaca agctcggcgt atccattggc
    54901 gacttgaatg aacgtctggc tgacaagctg gaggaactgg aaaccgagga cttggacacc
    54961 atccagcggc tggagcagga gttgaggcgc gactatcatg tcatcaccgc cgggaagcgg
    55021 ctggatcagg tggcgcggga tttcgtggcg cactattcca aggcctggga aagcggcaag
    55081 gccatgctgg tgtgcattga caaggtcacc tgtgtgcgga tgcacaagct gatcgagttt
    55141 tactgggagc aactgatccg cgagttggaa cgaggcttgg cggaaagccg ggacgaacag
    55201 gaggaagccc agacccgccg tcgaattgaa tggatgcgcc aaacccgcat ggctgtggtg
    55261 atcagcgaag agcaaggcga ggtggacaag tttcgcaagt gggatttgga tatcactcct
    55321 taccgccgcc tgatcaaaga gggcatcgaa ctgcccgagg ccatgcgcgg gctgccggaa
    55381 ttccaaaaca agcagcggtt ggcgctggac gatgcgttca aggcggaggc gcatccgttc
    55441 cgggtcgcca ttgtttgcgc catgtggctg accggattcg atgtgcccag cctgtccacc
    55501 ctctatttgg acaagcccct gaaggcgcac accctgatgc aggccatcgc tcgagcgaat
    55561 cgcgtcaatg agggcaagaa taatgggatg atcgtggact attgcggcat cctgaagaac
    55621 ctgcgcaagg ctctggctac gtttgccgga gccgcggacg gcggccgcgg tgatgggggc
    55681 gcactgccgg agccggcgcg ccccgagacg gagctgctgg ccgatttgga agaggcgcta
    55741 gggctggtcc gtacgttttt gtccgaacac gacgcaccgc tggacaacat cgtttttcgg
    55801 aagggttttg cgcgaaacgc agccatcgtg gcagcgaagg atgcggccaa cgaaaacgac
    55861 accgcccgga agcgttttga aatcatgtgc cgggcggtgt tcgtcaagtt tctggcgtgc
    55921 accaatgttc cggatgtcaa ccggtaccgg ggcgaataca gagccatccg gctgctgtac
    55981 cagagcttga aggaggatga agaaaaggca gatgtgatag atgtgatccg ccaactgcac
    56041 caagtggtgg ataaggccat tgtgacacaa gaccccgttt cagcagaggg aaaagaacct
    56101 tacgacatca gccaaattga tttcgacaag ctgcggcggg aattcgaacg cagcaaggcc
    56161 aaaaatacga ccgtgcaaaa tttaaaggct gcactggaaa aacgactgaa aaggctgttg
    56221 gaacggaatc ccctacggac gaacttccag acgcattatg aaaagatcgt ggccgactac
    56281 aaccgggaga aagaccgggt caccatcgaa aaaacctttg aagaactgat gagagcggcg
    56341 aaaacctttg acgaggaaga acaccgggcc atgcgggagg ggctggacga agaaacactg
    56401 gccctcttcg accttttgcg gaaacccgat ctgaccgccg ccgaaatcaa acggatcaaa
    56461 aaagtggccg ttgaactgtt gcagaagctc aaggcggaaa aactgcgcat tgaccactgg
    56521 cgggacaagg agacgacccg cgacgccgta cggctgacca tccgggattt cctatggagt
    56581 gacaccagcg ggctgcccgt cgaacgatac acggaaacgg aagtgcaaac ccgggctgac
    56641 gagatttatg tccacgttta tcgcgcgtat ccgaccctgc cctcgccgat ctacaatcaa
    56701 cagatggtct gttgactcat gagggggcag cggataaggg gcacccatta gactactcaa
    56761 gtgcgactac aggaggacca acagccccgg cctcaacgag gccggctaca gtaagacggt
    56821 tgcaggttaa aggttgcagg ttccagggga gcagccaaca accccggcct caacgaggcc
    56881 ggctacaata aagacaagaa agccaacagc ccgccgccgg ccttaaaggc ctggctgtta
    56941 actgtagccg cgggcggtga ccgcggggat tttcttccgg acacccgcgc cgccgcgagg
    57001 cggcggaggg ttcagaggtc agggttcaga ggtcaggtga agaccatcga accaacagcc
    57061 ccggcctcag cgaggccggc tacagtaaga cggttgcaag ttaaaggttg caggttccag
    57121 gggagcagcc aacaaccccg gcctcaacga ggccggctac aataaagaca agaaagccaa
    57181 cagcccgccg ccggtacaac ggcggcctac tggaaggtca gcagcccgcg ccgtcgtgag
    57241 gcggcgtgat tgtaaaaagg ccctgccggg ccttgtccgc tgtgcgcagc acgtgcatgc
    57301 ggaggatttc ttgcggattt tggcggggct gtcgcgccaa aataaccggt ggattttccg
    57361 atgatggcgc gttgcggacg acccgagctg gactcgtgtc cgcgagcggt cggtcgcggc
    57421 gcgccagcgc ggaaaagatg ccggttggtt ttggcgccgc caaaatccgc aagaaatcct
    57481 cctgatcaaa cgccgacacc agcagaccaa caccagccaa acgcgcgaac cacgagccga
    57541 gacgatagcg gcgggtgtcc tgaaggtatt tttccacggt gtggaaaaac ccgccgatgc
    57601 gggataaaat gttttccaca gtgtggaaaa atgggcatac tgccccccgc gtattgaaaa
    57661 atgcgcggcc aatttttcca cagtatggaa aaagtttggg cgatttttcc acacaatgga
    57721 aaaactttcc ggattttttc cacgcaatgg aaaaactttg gccgattttt tccacagtgt
    57781 ggaaaatccc gcccgtgcgg gatgaaaagt tttccacagt gtggaaaacc gcaggcaaga
    57841 ttgaaagagg agtgattcaa tgatcaaccg caaaccgtta gaggcgtcgt tggccgccgt
    57901 caaaaaggct tttccgaaag caaaaccgaa attgggcatg attctgggct cgggcttgag
    57961 cgagatagcc gaggcgttcg agcaactggg ggtgctgccg tatgcgaaga ttccgggggt
    58021 gggcgcggcg ggggtggccg gccacgccgg gcggctggtt tgggggaagt cggcgggaat
    58081 cgagacgttc attttccaag gccggcggca ttattacgag ggcgtgggct gggaggcggt
    58141 ggctacgccg ctgttcctgc tgaagagcct cggggccaag gcagttttcc tgacgaatgc
    58201 ggcgggtggc acgaatccca agtggaagcc gggcgatttg atgatcattg aggaccacat
    58261 caacatgatg ggggtgacgc cgctgttggg cacgcacgac ccgttctggg ggccgcggtt
    58321 tccggatcag tcgttcatct atgaccagca gttgcgcgcg gcgttggcgc gcgcgggcaa
    58381 gcagagcaag gtggcgctgc ggcgcggcgt gtattttgcc ggcacgggcc cgacctatga
    58441 gacgcccgcg gaagtccggg cgtggcgcaa gctcggcgcg gatgccgtgg ggatgagcac
    58501 cgtgccggag gccatgctgg ccaacgcggc ggggctgcgc gtggcggggc tctcgtgcat
    58561 caccaacatg gcggcgggca tcctgaacca ggcgctgacc caccaggaag tgtttgagac
    58621 ttcccaaaag gccatggcga acatgaagaa gctgatcctc caattctgga aggagctggg
    58681 ccgcgccggc ctgtaggccg gccccgcgca gcaacgcttt ttgcggaacg cttgaaagcc
    58741 cgggggggcg ggcgtatagt ttctcttata tgaaaacgat gcgtttggtc tgtttggggc
    58801 tggggatggc attgccttgg gtggcggtgg cggatgagcc gccggccgca ccgctggcgc
    58861 ccaaaattgt ctgcgacgag ccgagttatg attttggcga gcgcgacaat aacgggtttg
    58921 tggaacacaa ttacgtggtg cgcaacgccg gcacgctgtc gctggaaatc cgcgaggtgc
    58981 gcgcgtcgtg cggctgcacg gcggtgaagc cctcggtgga tgtggtgccg ccgggcggcg
    59041 aggcgcaggt gcaggcgcgc ttcgatttgc gcggccgcac gggctaccag caaaaggcga
    59101 tttttgtcac cagcaacgac ccggaaacgc cgacgttgac gctgaccctg aagggcaccg
    59161 cgacccagcc gctgcgcgcg gagccgtcca ccattttctt tggacaactg gggccggacg
    59221 ggttgcgcag ccgcgatgtc gaaattatct ccacgcgcgg ccccatccag attgagaacg
    59281 tccgcagcga ccaagcggct attcaagtga cgccgctgcc agcggcggat gatgcagacg
    59341 gcacgcggca ccgtttcaac gtgacgctgg cgaacgacct gccgccggga cagctcaatg
    59401 gcgtggtgat catcaaaacc gacttgcccg acactcctga actaacggtg ccggtgctgg
    59461 cctttattgt cggatccgcg ccggcccagt gatggctgaa agcacggcag ggcggcgcgc
    59521 ggcgttcctc cggatgcttc cggccagcgc gactttgctg gcgctggccg tggcgctggg
    59581 ggttgcggca aatctggtgc gcccggcagc aacgcgcctg ccgtgggcca gtgattggag
    59641 ccggcacatc gaaacccaag cctaccgcgc cggcgtgccg gtgctttttc tgccgggtgt
    59701 gcgtgagcgc gcgggcaacc cggattggtt gatttgcgat gcgcgaactc ccgaacaata
    59761 tgcggcgggg catttgcccg gcgcgtggaa cctgccgctg gaagaagtgg atgcgcggct
    59821 gggcgcgctg gctgcgcgct tgacgctgca gtcggagctg ctggtatatt gcggcggggc
    59881 ggattgcact gatgccttgg agctggcact caagctgcgg caactgggct ttgaacgcgt
    59941 gacgctgtat cccggcggat acgcggagtg ggtggaatat ggcggtgaag tgcgcacggg
    60001 agacgagccg tgagccgcgc gcgaatcatt ttggtatggg tggcgcgcct cgcggtggcg
    60061 gggatgttca ttggggcgtg tctcgccaag attgcggcgc cggaatcctt cgcgctggcg
    60121 gtgcatcggt atcggttgtt gccggatttg ctggtgaatg cggtggcgct gctgctgcct
    60181 tggctggagc tgttgtgcgg cgtagcggtg ctggccgggc cgccgcgctg gcgcgccgcg
    60241 ggggcgctct ggattgcgct gatgttggtg gtcttcaccg gcgccatcag cctgaacatc
    60301 ctgcgcggga ttgaagcctc gtgcggttgt ttctcaacgc gcgccgatgc ggcggcttcc
    60361 gacggctgga atctggcgcg caatgcgacg ctgctcgggc tgacgctggt gattttgtgg
    60421 gacgcgctgc ggcgcgacca gtccgctgcc gcgggcgagc cggcaccatg agccaaaacc
    60481 tgcaatccat ttttgaggtc tatgtggccg gcgtggggcc gtcgagcagc catagcatgg
    60541 gcccggagcg cgccgcgcgg ctttttgcgg cgggcttggc ggaaactccg gcgcgcattc
    60601 gcgtgacgtt gcagggcagc ctggccgcaa cggggcgcgg gcactggacc gatcgcacgc
    60661 tggtgcgcac gctgaatccc gtgccggtgg acgtggtgtt tgactcgcaa accaccaacc
    60721 tcccccaccc caacaccatg atttttgcgg cgttcagtgc ccaaggcgac ttgttgcggg
    60781 aatggaccgt gtgtagcgta ggcggcggca gcctgcggga ggcggatgga cggccggtgg
    60841 cgcgcgcgga gccggcggag tatcccgtcg gcaacatcgc ggatgccatg gactactgcc
    60901 acgcgaacca tctggagttc tggcaattgg tcgaacagag cgagcgcgac gtttggcccc
    60961 ggctggagaa catttggcgc gcgatgaccg ataccgtgaa acgcgggctg gccagcaagc
    61021 aaatcatttt gcccggcacg ttggagctgg cccggcgggc cggaaccacg catagccgcg
    61081 cgctggggtt aaaaggcacg ccgcgcgaat tgggcctggt ggccgcctat gcgttggcgg
    61141 tggcggaaga aaacgccgcg ggcatgcagg tggtcacggc gccctcgtgc ggcgcggcgg
    61201 gggtgttgcc ggcggtgctg tactatttca aggcgcggcg tcgcgtgcca cagcggcgaa
    61261 ttctggaagc gctggccacg gccggcttgt tcggggcctc catccgcgcc aacgcaaccg
    61321 tctctggcgc ggaggccggc tgccaggcgg aaatcggatc ggcctgctcc atggccgccg
    61381 ccgccgcggc ccaactgctg ggtgcaacgc tccgccaaat tgagtatgcc gcggaaatgg
    61441 cgatggagca caacctgggg ctgacctgcg acccgattga aggctatgtg cagattccgt
    61501 gcatcgagcg caacatgaac gcggcgctgc gcgccattga gtgcgccacg ttcacgcagt
    61561 tgaccgacgg ccggcatttg gtcagttttg atgatgtgat tgaagtgatg aaccgcactg
    61621 gccgcgactt gcaggcggcc tatcgcgaaa cctccacggg ggggctcgcc cagctttggc
    61681 ggcgggatat tgaaacccgc ctgcgcaaac cggcccgctc atgagtccgc gggtcaatga
    61741 gttctcgtgg tcgttttcgc ggcaggcgac gttccagtct tgcctgaagc gctactattt
    61801 ttcctattat gcggcatggg gcgggtggag tgcgacggcg ccggcccgcg cgcgggagat
    61861 ttacctgctc aagcggctgg ccacccgcca gcagtgggct gggcagcacg cgcatgacgc
    61921 cttggccgcg cttctgcaac ggccggcggc ggacgctgcc gaagccgaga gcatcgcgca
    61981 acagcaaatc gaacaaatgc gccaggagtt tcgccagtcg cgcgcgaagg cctatcaccg
    62041 cgacccggcg cgcattcccg ggctttttga gcacgaatac aacctggaga ttcccgccga
    62101 ggaatggcag gcggtggtgg aacgcgtagc tcaagccgtg cggcaatttg cgacatccga
    62161 actctggcag gagctgcaac aactgccgcg cgaagccatg gtcgcggtgg agcagcgcgc
    62221 gcactttttg ctggatggcc tcaaggtact ggcagtgcct gatctcgtgg tgcggcacga
    62281 gggccgggtg actatttatg actggaaaac cggcagcgca gatttggccg aacaccggct
    62341 gcaactgggg atttatgttc tgttggcgct ggatcggtgg accagcgcgc cgagcgacat
    62401 ccaagccgtt gcctaccacc ccattttgaa ccagcgcgag gtgttcagct acacggcgga
    62461 tgatgtggaa aacctgcgcg atttcatccg tgactccgct gatgaaatgc tctttcccgt
    62521 ggaagacccc atgaccaacc ggttgagcgc cgatgcggag tttgattgca ccaccgatga
    62581 caacgtctgc aagacgtgtc cttttttgcg ggtctgcccg aagtgggcca cgtaaaggcg
    62641 tcaggcggcg gcagcttcag ggcgtctggg gctttgctcg cttcttgaat tccgcgcacc
    62701 acgaaaatct cgctgtcact cgtcgcattg caaccggttg gattgttggg ctggaacggt
    62761 cgcagacctt ccagcctttc ccggcgggaa ggtatcgttc ct
//
LOCUS       DAOPNP010000003        49799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_897_length_49799_cov_8.416251, whole genome shotgun sequence.
ACCESSION   DAOPNP010000003 DAOPNP010000000
VERSION     DAOPNP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 49799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49799
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_49799_cov_8.416251"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="PKI64_00640"
     CDS             <1..2187
                     /locus_tag="PKI64_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99544.1"
                     /translation="GDDGYRINRINNVNCFVINTAADDYRFANHDSGLPMPMRELFSR
                     RARAGRQDQISTIFTMWEEFSNLSRADAYDRNLRWLANRPWIQLVSLEDIAAGRIALP
                     WNQSWEPIDRGNTAGAKQSHDWINHANNENYDNWYNGSWRHEGLVGKKFELRPGVTNA
                     TAYGMVYTGGIISNTWTKVAGITNADIKRLAREVLHASVFETAFHNENNNDLTKWSIG
                     GYINPASDNQTLIGFAKQAQSQTRLAAIYSWADSWATVAPGLATTQTASVDVDQDGEN
                     EYVLYNKHVAAVFERIGGRMIAAWHRDGDGRIRQMIGNLASCAGSETEVEGASNVDTN
                     TGAVVAYRTSALKDWYAGTAQYVNDLYTVTTNGTDGWKLTSSDGKISKTVTLAPTATS
                     FAVQYTINPSLNGGVLYVRNGFSPNLSELLLAGQRGFVDTISATGGVVALTTTNNHSG
                     VLLTMTQGQVNTAARDDHQNHEYDSVRMRNQAQTRQVELMGTNSIAFSIDLFAEDANN
                     EPPVIEFTPQGPYTNAVGTTNTFTVSVTDPEDDPVILTVGTLPFTATFNPETGVFAWW
                     VTNMGSAGSTSVVTFVADDGVNVVSNYATIVVPWDANGNDIPDDWEFRYFNGDLSQTK
                     DGDYDNDKFSNFAEWLASTDPMAAGSYIGWEMQMVVNDGMMLRFQSVDGRTYHIEGND
                     GDLPNAAAWHHLATVYGTGTQTDWVDNAYPTNAVRHYRIKIPRHVP"
     gene            2668..5826
                     /locus_tag="PKI64_00645"
     CDS             2668..5826
                     /locus_tag="PKI64_00645"
                     /inference="COORDINATES: protein motif:HMM:NF039178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HOD99545.1"
                     /translation="MQYSKAVFGKDGGVILNIILINNLIGKMKKSSSNKTDQTVIVAN
                     YAVIRLLVVVGLLLGATIADAQWRITNSQLPVKKATQKIDFPPIPDHITTSTVALVAT
                     ASSGLPVEFSVEEGPAQLAGNVLTFRGAGKVTVKASQPGDEHWAPAKDVSQSFRVSTA
                     EAKIVLSALEQAYNGRPRVVRVETMPPDLRVLVTYDGKKEPPIDAGKHSVAVMVVDPM
                     WHGKAEGLLVVRPGKQVIDFPPIPDQTTSSTVLLRAKASSGLPVEFMVLEGPAKLEGN
                     LLYFTEAGVVVVKASQPGNNNWAPANDVLQKFEVINDQAVVILSNLDQVYDGTPRAVQ
                     VDTKPPDLKVLVTYQGDPKPPINAGAYKVRAVVKDPFWTGEAFGTLSIKPAEQFIDFP
                     QVPDQVATAVIRLEAKASSGLPVQFSVASGPAVWDGRFLSFTGAGSVTVVASQPGNGN
                     WLPAPKVANRFEVIKDTAGIMLYDLRQLYDGTPRQVGVRTKPAGLPVKVTYNDDPAPP
                     VNAGKYWVVAMVADPRWEGRAEDVFFIDPAKQTIDFPEIPPQLASATIELEAKASSGL
                     PVEFAVDSGPGQLVGNLLSFTGAGPVTIKAMQPGDNNWLPAESVGQTIKVIPDQAAVT
                     LSNLTQVYDGTPRVVSVTTDPPGLAVEVTYDGSLNVPVNAGTYQVVATVVDGRYEGQA
                     RDVLTVEPAKVTIVLDDLEQVYNGEPRAVTVFIEPEGLPLEILYSQLGLVILGKELPS
                     EDPPVDAGDYLVSVTVVDDNYEGRVEDKLTILKASQVIDFAPIADCWTTDIVELDATA
                     TSGLPVSFEVVSGPALLEGNILTFTSAGTVEVQAAQEGNGNWNEAEPVAHSFEVKASN
                     HEQEGPYTYIVEDDEATIVGFDESYQGTLALPGSLGGFPLVGIDAGAFANSSGLTSIF
                     IPGNITWLGEDAFTGCTNLERIYFLSDVLAGEPASLGASRATAYYLPGSEGWGATFGG
                     CPAVLWNPKFVNGYCSPLAENCQVVGAPNLPIGIQSSTNLLAGEWMWEVTLTNLTAEG
                     TFCFAKYLADVEEERYYRIIAP"
     gene            complement(5969..6871)
                     /locus_tag="PKI64_00650"
     CDS             complement(5969..6871)
                     /locus_tag="PKI64_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009571128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOD99546.1"
                     /translation="MIAELMKFHPDVANALAAGRPVVALESTLITHGLPHPINLEVAQ
                     AMEQAVRDNGATPATIAVLRGQITVGLTSEQLTYLAQATNVRKCSRRDFPIVLAQKGD
                     GATTVAGTMIVAHLAGIKVFATGGIGGVHRGHPVDVSADLLELAQTPVAVVCAGAKAI
                     LDLPLTLEVLETHGVPVVGYGCAEFPAFYSRSSGLPLDVRCDTVEEIAAMIRAREQLG
                     FRTGMLITNPIPAADELPAEIAEAAIQQALQEADAQGIRGKETTPFLLARVSELTGDR
                     SRAANIALLKNNARVAAQIAKAIV"
     gene            7185..13223
                     /locus_tag="PKI64_00655"
     CDS             7185..13223
                     /locus_tag="PKI64_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD99547.1"
                     /translation="MKSTVRNWLMVFLTTGLFALTAAAQPIYLNAGVIDTATTEPRKA
                     VAGTTGDQLHLVQFDGPIQPEWVAQLEAAGWRIVDFVPDNTYIVYGGQTARKKLRAQV
                     SHVQWEGAFLPSDKIQPRATPAALKQRKLTKPDSKLFAVQLVEDAAANAATIAWLEEA
                     AGEPLASIATNETLRFVNIRVALPENRIEELAGRQDVLVIDVYEPPQRRGERQGQIVA
                     GNVNAAGSQPTGPGYLSWITSKGFTQAQFDASGMVIDIADDGWDRGVAANPANREFRV
                     DGDAAKSSRVKYSRTASKLTEANSAGTDGHGNINISIIGGYNNSSGNPYSDADGYHRG
                     LGINPFALMGNTKVFADGGDWDPSEAQEKIFIATNYHSGVRISSDSWGLDGDGAYNVD
                     AQNYDTWTRDSQPGVSGNQQVLYVFAAGNDGPGSKTIGAPGTGKNIFSIGASENYNMF
                     GTDGCAVGNTGANNANDIIDFSSRGPCKDSRKKPDIVAPGTHVAGAASFANGYTGEGV
                     CDKYQPAGQTNYAASSGTSHSTPAVAGGASLVYQYFINQGWTIPSPAMMKAYLMNSTR
                     YLTGVDANDTLPSNNQGMGLMNLGTAFDGTQRMLRDQLTNDIFTASGQTRTFFANVVE
                     TNKPLRITLGWTDAPGSLSGNAYKNNLDLTVLVGGQTYLGNSFAGAFTTPGGTADPRN
                     NVESVFLPAGQSGLITIKVTAANINSDGVPGYGGALDQDFVLVAYNAEEYIPSNYPPV
                     LEPIGNKAMATNRLMQFTVTASDPVDGDSVRLWATGIPAWATFAGATNAGTASAQFSG
                     TTPETTGTYNVAFYAADKDGTNTESIVIVVNDINCVPAVLLSEDFDDSTGVPTGWVNS
                     GSANDTSATHYKSSPNCRGFGAGASLETPPINTPTQIVFYVDASGNGAGKTATLDYKI
                     GSGEWKKVGNFIATTAGKTETFDLTSSPDLREQANVSFRFSSTFSTWYLDDVIISGMD
                     CGGGFLPNNPPTIGVEGVGGTNKAGSVGVELSFTVTANDADSDNVSLQATSLPAGATF
                     ATASGPAPVQSTFTWTPGSTGKYSAVFTANDGKATATQAVSITVSAFVAPLLAPEILA
                     PSAVLANQFNANWSAVSNATGYRLDVSDSPSFSGGGGGLVLAEDFAGVTVSNANDISA
                     SLDTYTLVPGWTGAKIYPEMGWIKLGSASAKGYITTPTLDLSGNGGVATLTFELAKYG
                     TDATTVQVLHAPDGSNFTQVGSDLTAPSSKTLQTIQINDGTANSKIQISAKGLSKSRF
                     YLDNIQVEGGGGAGNFVPGYQNRDVGNVTTYAVTGLAENVTYYYRARAYDASRNSDYS
                     ATTNVTTTAGVDIPPVLNPIGNKTVALGGSLQFAITAVPTDGDEVTLTTSALPAGATF
                     GSTNENGTFQWNNASPTGVYSVTFYAEDVDGQDSETITITVYDPEAELQAPVVQAASS
                     VQAQQFNANWLASVGAVGYRLDVATDDSFTAGGGGGGGGAVLSEDFAGFTAVDGTQDR
                     SGSLNTYLQTPGWTGSKVYEDAGRAKLGSSSALGYIVTPTVDLSGNGGNATLTFDLGQ
                     YGTDVGSIQVQHAPDGSTFSPIGSDLTPPASMTSQSIPITGGTANSKIKFTAKVASKN
                     RFRLDNVEITAGSGGGGGSGSFVAGYEDRDVGNVTTFAVTGLTESITYYYRVRAYDAS
                     TNSGYSATTNVTTVAAPALQPPVIQAASGVQAEQFNANWLASVGATGYRLDVGTNETF
                     SGGGGGPSERLLLATNAASSASIQTDGWSATDTGGSTYVQMAKPTATILSPAFSTVGF
                     TNLTVDMMARTYNGVNATYNIITVSVSTNNGTAWDTLGTVLPLSSTMANMGTLTDATH
                     IGHAETRLRWQTLGANGTIGAGVQQLHIRGWTAGGGVAAAYVPGYENRDVGNVTTFAV
                     TGLVEGVTYYYRVRAYNASTTTVHSAVTSVVTSASTPTQPEFDTFTFPPNATPAATFA
                     SQAGITYQLQYTTDLLANPPVWTAADTEVGTGGNVTLEDTNAVDSRRYYRIVIP"
     gene            complement(13306..14520)
                     /locus_tag="PKI64_00660"
     CDS             complement(13306..14520)
                     /locus_tag="PKI64_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD99548.1"
                     /translation="MYDILIKNAQLRRKAGLWNIAIEKGVIQKITKQPIRGKAGKVID
                     AGGNLVTEPFVNTHLHLCKVYTLEMMDSLALRAYHGADMGGAMTAIELAARVKENYNE
                     KWILPNVRKALKLAALHGNLHIRAFADVDSKAKLIGLKALLKARDEFKGIVDVQVCAF
                     PQDGVVREPGTVDLMKEAMDLGADVVGGIPWIEFTEKDEQIHIDEMMKLAIAYDTDIS
                     MLVDDAGDPTLKTLEMLAVRTIEEGRVGRNLAHHARAMSLYPTPYFKKVAALLRQAGM
                     GVVSDPHTGPLHARVKELLEEGNLVCLGQDDISDAYYPYGRNNMLEVAFLASHLLWMT
                     TYPEMETLYDLVTVNPAKCINLRKFELKEKNNASLVILDAKTVCEAFRWHKAPLHVIA
                     NGKLIKADNLRG"
     gene            complement(14560..15330)
                     /locus_tag="PKI64_00665"
     CDS             complement(14560..15330)
                     /locus_tag="PKI64_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOD99549.1"
                     /translation="MSFSHEERQRYARHLDLPGFGEAAQLRLRAGRVLVVGCGGLGSP
                     ALYYLAAAGVGNIGLMDPDLVEISNLQRQILHATPDIGRPKVDSAITRLRALNPHVRF
                     TAHAEYLTAENARALLAPYELILDCTDNFAAKYLISDTCVALKKNFIHGSISGFCGQV
                     LTVCPGQTACLRCIHPTPPPVDDPAAAHTAGPLGAIPGVIGSLQAVEAIKVLARIGNT
                     LTDTLLTYDGLEVEFRAVRARRNPQCPACGDAAAPVNV"
     gene            complement(15338..15868)
                     /locus_tag="PKI64_00670"
     CDS             complement(15338..15868)
                     /locus_tag="PKI64_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOD99550.1"
                     /translation="MANWYFVNPKTREKVGPNEEAHVRAKFIAGELPPHTLVWHDGLA
                     NWIPASQAFAALKAPAGSEGKVPLPDGLRGWMTFIAIMMILSALLPSVMLFGIPMLVA
                     GIALLGARAALDRAPFIAPDMLPFFSKLRTFFCCWGWMYIIGAFLAVLLLLLYVGVVF
                     VALSSGDSATPFLPLK"
     gene            complement(15958..17214)
                     /locus_tag="PKI64_00675"
     CDS             complement(15958..17214)
                     /locus_tag="PKI64_00675"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOD99551.1"
                     /translation="MNLHDTMLQMGDQAVTAARQLATLGSRRKNIILEAMAESLEANR
                     DRIKEANARDMAAGREAGLSDAMLDRLLLTDKRMDSMISGVRALIGLKDPVGQRISRW
                     IRPNGLVIQKIRTPIGVIGIIFESRPNVTVDSAALCFKTSNAVILRGGKEALESNKAL
                     VDTLQAGGTRKGLPANAIQLITTTDYDAIRELVQLQGRVDLVIPRGGEKLIRAVTEQA
                     HVPVIKHYKGVCYAFVDESADQAKALDIIENSKCSRPGVCNALEKVLVHEKIAAEFLP
                     KLAARLTDKRVELRADEASRAIVPALAPAAPADWDEEYLDLVMTIGVVPTLEAAIAHI
                     NRHGSHHSDAILAEDTKAQDKFFAEVDSACVYANASTRFTDGGEFGMGAEIGISTDKL
                     HARGPMALEELTTYKYTIHGTGQIRS"
     gene            complement(17247..18404)
                     /gene="proB"
                     /locus_tag="PKI64_00680"
     CDS             complement(17247..18404)
                     /gene="proB"
                     /locus_tag="PKI64_00680"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOD99552.1"
                     /translation="MPHQTARKALAASRRVVVKIGSRVLVQRNGRPDTRRMTALVKDI
                     AALRRAGREVIVVSSGAIASGLQALGIKKKPADLPTLQMAAAVGQSRLMAAYDRLFTR
                     QKCTIGQVLLTHDDLEHRRRHLNARNTMLKLLEHGIIPIVNENDVVSVDEIKFGDNDA
                     LASRSAMLVEADLLVLLTTVNGFHAPDAKGRSRRVPYLADVTADTLSHTQGKGSIFST
                     GGMASKLTSAAEAARMGTAVVIANGRKDDILQQVVAGANVGTLIPPAPAPTANGGLNL
                     RRRWVAFFHHAQGKVTVDDGARDALLLRGKSLLPVGIRAVTGDFDAGDLIEIVDAAGR
                     CIAQGLTEFSSTETQLLKGLKSGKIRELLGADAPDEVIHRDNLVLLTPPAR"
     gene            18527..19468
                     /locus_tag="PKI64_00685"
     CDS             18527..19468
                     /locus_tag="PKI64_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD99553.1"
                     /translation="MNPEPAYRYLFGPVPSRRLGRSLGVDLNPLKVCTENCIFCQIGR
                     TTELTAARREWVPTADVLAEFDRWQAAGGTADFITLSGSGEPTMHTGFGEVLAHVRAA
                     RQFKTALLSNGSLLWLPEVRQAAVLADVVKVTLSAWDEESFARIHRPAAGLTLARLLA
                     GARALRAEYGGQLWVEVMLLAHINDAPEQVRAIAELARTLAPDAIHLNTVTRPAQAGV
                     AVSRVGAAWLHTVAPWFQPVAEVPEFTGQIPAPEQMTDAEWVESLARHPLDLSALAAA
                     AGVPVEAVEQRLRGLVAAGRVVIEEREGQRVARVATP"
     gene            19465..19743
                     /locus_tag="PKI64_00690"
     CDS             19465..19743
                     /locus_tag="PKI64_00690"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOD99554.1"
                     /translation="MNLTRQQVRVRYTGDVQGVGFRYTVRKLATPLGVTGGVENLLDG
                     SVQMVAEATPDTLRLLLTQIHASRLGAHIADEQIAWGPAQGLAGFYYR"
     gene            complement(19734..20624)
                     /gene="dapF"
                     /locus_tag="PKI64_00695"
     CDS             complement(19734..20624)
                     /gene="dapF"
                     /locus_tag="PKI64_00695"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOD99555.1"
                     /translation="MAFVANAPPGGQLCFMAARAARLVFARRPWHRDAMQWSFWKMHG
                     AGNDFILLDDRALAFPATDRAFIAQLCDRRRGIGSDGLLLIQPATGADFRMRFFNPDG
                     GEADLCGNGARCVARLAHELGIAPAAMHMKSPAGIIRAEILSPLVRIHLPPPKDWRIN
                     LSVEWNGAAWPLHFVNTGVPHAVLPVADLAAVDIPQLGAFIRRHELFAPAGTNVNFIR
                     VLDSHRLAIRTYERGVEAETLACGTGITAAALIAARVGLASFPVQVQTASGDVLEVAE
                     SPLTLTGPAEHAFRGVVDYR"
     gene            20627..21601
                     /gene="galE"
                     /locus_tag="PKI64_00700"
     CDS             20627..21601
                     /gene="galE"
                     /locus_tag="PKI64_00700"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOD99556.1"
                     /translation="MKILLTGGSGYIGSVTTELLCDDGHEVVVFDNLERGHREAVDPR
                     AKLMVGDLRDPQPIREAMRQAKPDAVVHFAAYALVGESMQDPHMYFENNLGGGLNLLA
                     AMLESGVKKIIFSSTCATYGQPEVMPMTEALPQKPTNPYGESKLMFETALRWAQERNG
                     IQPVFLRYFNACGATEKYGEDHDPETHLIPNVLKVALGQAPAVPVFGNDYPTPDGTCI
                     RDYIHIVDLARAHILALEKEVTGAFNLGTGTGFSVQQVVETCQQVTGHEIPVALSPRR
                     PGDPACLVAGATKAHEVLGWQPRYPDLQTIVQHAWNWHHAHPHGYRKE"
     gene            21603..21986
                     /locus_tag="PKI64_00705"
     CDS             21603..21986
                     /locus_tag="PKI64_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99557.1"
                     /translation="MSRLPVQTEGFRVDTSRPRSRTKMRAKFRTVLGVSAGVLPLMAL
                     VIFLMSFLQIREQREPLHLLALILLGSGMVVLMFYAWARAEKLRRREISARNREARYR
                     EQAEHLRQEREKKAAERSAGNAQVS"
     gene            21986..22960
                     /locus_tag="PKI64_00710"
     CDS             21986..22960
                     /locus_tag="PKI64_00710"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOD99558.1"
                     /translation="MPRHPAARTGMALILVLAAIVLAGGLALYLQARAAAAARLEQAE
                     LTRERLRLAAGEAAREALWLLAADDDRQVDHLGEDWAKPHENQRDDGVYTWSLIEDAG
                     RFFNLNNLAVATQGPRPANEILLDLLTFCGDFSPSVRVAALTDFVDGDEDGPYEAEFY
                     RRPTDPYRPPNRELWAPAEVLRVHDFTAELFRPRPRGPADDLFGGDFSSAAVIVPQRL
                     NKPVPINVNTASRDVLMGLTSLQHDAVVRKVMAIRQTQPFESLAMIFAAYPEFAAALA
                     DSVSTASTIFRVRARAAADSQRWSVMAWVERSPDTGAIRILQWVEAGG"
     gene            22965..24362
                     /locus_tag="PKI64_00715"
     CDS             22965..24362
                     /locus_tag="PKI64_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99559.1"
                     /translation="MEHPFQMAYGLDMADGQWILAQCARRGVAQVRLAAPAESEEMRR
                     ALQAVQADVEQGRAALAVSAPAAQTVVRRLRAPFPSLAKAARIWPSLLDVDLPFPVES
                     AVTGFVQPRVVQGQATTLAAAIRRSDWSAFEVQCNAAGVQPTHADVEALALWEQQSVD
                     APSARADASRLLIWLGADHVTIVRGRGGDLLGAHVLRATPQPSGSGDFVTLWAARLQP
                     IVAAQATETDGAPMDVWWAGPRAEDAEWVARLRAAMPAVGQMRHETHSLAASLLARAL
                     ARRAAGNGGINFKSGADTHPALHRAAQRRRQRAYLGVVIAALVVLALNFGERHLRQRQ
                     LSAAQQNLSHLAESITGAPVVRGQELLFVERALAQRDEDTRAFREAMEEDGLEERVAR
                     VVQAADELHVEIVRLNISPVALTLQGSAPGIQVLETFAEKLRGYGWQVQADTSGLALG
                     GRPQFVLKGMHDQVR"
     gene            24346..24840
                     /locus_tag="PKI64_00720"
     CDS             24346..24840
                     /locus_tag="PKI64_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99560.1"
                     /translation="MTKSAERYWQAGALLLGVLAVAFTLDTWLSAARHRDILAAKEEA
                     GQKVAAYAGRWAREDAYRDSLERQQAWHPVELDDLATRLLGLHTVQITPRPATAVAAG
                     WQRREVAIQMNNVTYAEAALFLADAAAGLPPWRLQDLTLAPAAEAGKGNLAAVLVALE
                     KKQP"
     gene            24857..25657
                     /locus_tag="PKI64_00725"
     CDS             24857..25657
                     /locus_tag="PKI64_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99561.1"
                     /translation="MKLLPTIWNRRELLWNLAVRELKQRYRGSVLGFLWTLLTPLFMA
                     VIYLFFLRLLAGRGVPMEEIIIGVFAWQFTVQCTQAGTTCVLGSANLVKKVAFPLEIL
                     PLSQVLANTISYLLSLVVQFPLLMFLLSRSNQHLAMTWPIFFLWVAWQTLFNLGLTSF
                     VGALNVFYRDTQHLVGLALSAWFFLSPVMYNMEFALNLAAQWPLAADLLYANPMTVIL
                     NGYRAALLPESAAAYPPAAWIGLLLVPVMFILGVVVYRRRQHLFADML"
     gene            25659..26834
                     /locus_tag="PKI64_00730"
     CDS             25659..26834
                     /locus_tag="PKI64_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99562.1"
                     /translation="MNAIEAVGLGKRYYRRGGQRATTLLETLTGFGRARREEFWCLRE
                     ANFAIPAGRTVGVIGPNGSGKSSLLGLAAGTITPTEGHVRTQGRISSLLELGAGFHPE
                     LSGRENVYLNAALLGIPREDVKKKFDQIVDFADLREFIDMPVKHYSSGMYVRLGFSIA
                     VEVDPDILLIDEVLAVGDIAFQLKCLDRIRQFQRRGKTILFVSHALQTVEEFCHEAFL
                     IHGGRLVERGEPSEVILKYIRRYMGEGGGLFTQEFGTRAVEFGNVRLLNAAGEETGIF
                     ETGGALTIEIPYVAHRRIEKPVFGFSLKTGNGMYIYGSNTQIQQVAVPPLEGAGVVRL
                     DFAPLRLMHGNYFLSLSIHSWDHAEQFHRREDWWPFGVRNTGEDLGLFKLDARWHCP"
     gene            26970..27671
                     /locus_tag="PKI64_00735"
     CDS             26970..27671
                     /locus_tag="PKI64_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD99563.1"
                     /translation="MKKETRRWMVWWLVVGMAGMVLALTGCESDHNDETMEEIQAEAT
                     NSVNNADSPGDEDHGLNEFFYDGLHPTRAGNKVIARVFAKKIKPLDDAGGNNVNVIVC
                     LGDSITDTGYPSDLAKLTGMTAYEEGIRGERSSGGASRIDGVLSGYKPAYICILYGAN
                     DVIGHLPASTVTGNLEAMVIAARANGTIPILATLTPMFGPRKQYAADARATSAAIRDL
                     AGRLGVPLADLEKAF"
     gene            27689..28429
                     /locus_tag="PKI64_00740"
     CDS             27689..28429
                     /locus_tag="PKI64_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD99564.1"
                     /translation="MQKRRQWAWWLTIGLCGTLLLATGCESDDSHETPAQIQEEAQNT
                     PPVTNPGGNGPSESTTGTPSTSPPSAATVRKYFPGGLHPNTEGSRIIAKTFAGAITPV
                     SDAGGNRPNVIVCLGDSITDSGYPTYLAGMTGMTAIEEGIRGERSAGGVSRVGGVLSK
                     YKPAYLCILYGANDVRKGLPASTVTGNLEKMVNAARANGTIPILATLTPMGGTYSEFI
                     PEVQKTSAAIRDLAARLGVRLADLERAF"
     gene            28522..29928
                     /gene="murF"
                     /locus_tag="PKI64_00745"
     CDS             28522..29928
                     /gene="murF"
                     /locus_tag="PKI64_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOD99565.1"
                     /translation="MDPVAPDFAASACRGEWQPHPPAGMLSAIGVDSRTVARGSLFFA
                     LPGARADGHDFLADVVAAGAHAVLRADYPVDKLPTGGHFLRVDNVLDALGRFAAAYRA
                     TLPTRMVGVTGSVGKTSTKEIIADLLCTLGKTGRTVGNYNNEIGLPLSVAALDRECRF
                     GVIEAGISHPGELAHLRDILRPDMAVITRIGPVHAEFFDSVRDIAEEKATLLTALPAD
                     GFAVLDRDDEFFPMLRARCSAPVITCSLRRREVDYAGDLQEDDQLWVCEAATGETAML
                     PVPPPASYMAENVLRAVAVARRCGATWPAIAAALAQVKPVGMRWAVSEWQDITIINDA
                     YNANPISVRAAVQAFAAWPVRGQRFLAIGPMLELGRLEATEHEALGRHLATGQWAGIV
                     LVPWKIHVGAPDPATQALAAGLQAGGWPAEKMCLAPDAAAAATWLQGRCRAGDALLLK
                     ASRGVRIETVLTHWQKES"
     gene            29933..31096
                     /gene="mraY"
                     /locus_tag="PKI64_00750"
     CDS             29933..31096
                     /gene="mraY"
                     /locus_tag="PKI64_00750"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD99566.1"
                     /translation="MLYYLSLLSEHYSPLNLFRYVTFRAFMGAGTSFLLSLLLGRWMI
                     GRLRAYKIGQMVRTDAELAAINHLGKKGTPTMGGLLIIFTTVLAALLWCHSSNSQVWL
                     VLATMLFMGGVGFVDDWLKLKKRGSEGLREWQKLALQGVWVVIAFVVIEAVPATHFYA
                     RQLMVPFLKTPLFTDITVPGALLFMFLVLVGTTNAVNLTDGLDGLAVGCVNSVSAAYL
                     ALAYVAGNIVMATYLQVPAIAGAHELAVFSGCLLGAGLGFLWWNAYPAKVFMGDTGSL
                     ALGGTIAMIAILIKQELTLLIVGLVFVAEAGSVMIQRAACRLYRWRTGTGLAQDRRPF
                     RMTPLHHHFEIISKERAKAEGRSPDAAENSVVIRFWIVSIVCALLGLATLKIR"
     gene            31102..32421
                     /gene="murD"
                     /locus_tag="PKI64_00755"
     CDS             31102..32421
                     /gene="murD"
                     /locus_tag="PKI64_00755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOD99567.1"
                     /translation="MQRYSHAMVCGLGASGVAAAQLLRREGTTVIAVDEFDTAATQHA
                     AETLRAAGCRVELGCAAPPEVTVDVAIVSPGLALHSPLLVALQKRGIPLLSEMELGWS
                     RHAGRTVAVTGSNGKSSVVKALADCFAAAGHKAVPCGNYGLPVCEAVMTDTEWLVIEA
                     SSFQLETCAGFRPDIGIVLNIFPNHLDRHGTLETYAKLKARLLSRQRAGDAAWVPPEW
                     AASLRAWSQGQGTWHTFGTTTQEEVHYAAHQLVARDSRAFNIAGSYFDNEVLGANAAA
                     LLGAAQAAGLPPALVAQALVNFQPLPHRAELVGEINGVRYVNDSKATNLSAMLAAIRM
                     QRGAVILIAGGRPKEKDFAAAVPLLRERVRRVLLIGEAAAAMQAAWGAHVPCTDCHTL
                     AQAVAAARQTARPGETVLLAPACTSYDQFRAYPERGEAFRKYVTEKD"
     gene            32499..33419
                     /locus_tag="PKI64_00760"
     CDS             32499..33419
                     /locus_tag="PKI64_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD99568.1"
                     /translation="MRVSVVASLILAVHVAVIGSVVMTQGCASTRQGASTTPAQVDSP
                     PVPPLPPVAVDMPTVAQPVAFPVIQPPAPPAPLKTSVAAENVYVVKPGDILSKIAAAH
                     GVKTAELKELNQIADANKIRVGQKLILPDYAKPSQSQPAAKESKAAAAPVAAGGDTYV
                     VKSGDALSKIAAAHGVKVKDLMAANNITDANKIRVGQKLTIPGAKAAEKTEKADKPEK
                     TDKPAKPKKAESEAEVAPIEPVAASPAEPANVPDQDKSLEYPAAPGDTIESIARLFSV
                     PVSAITKANNLPVDAKLEPGQKLLVPQTSP"
     gene            33495..34598
                     /gene="ftsW"
                     /locus_tag="PKI64_00765"
     CDS             33495..34598
                     /gene="ftsW"
                     /locus_tag="PKI64_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOD99569.1"
                     /translation="MTSRVLLILVVGALVLFGLVMLFSTSWPQGEKISNNQYYFVQRQ
                     AIWLALGLVAAVVGSRVDYHVWQKIAWPIMGGVLVLLALVYVPGIGLSIKGSRRWLQL
                     PMKLTFQPSELAKFAAINALACWYGARRRGGGTFTRVVVFPALILGSFLLLILPEIDV
                     GATVLIGATGGVIMLVAGAPFWLLAFGVALAAGGLGVLIKYDPVRMSRVLAFLNPEEY
                     AQTEAFQLLNALYAFVAGGPTGVGLGQSLQKQHYLPEAHTDFIFAIIGEELGVVASLS
                     VVVLFVILLLCGIRISGRAPDVSGRLLAFGITFMITLQAAINIGVVTGCLPTKGLPLP
                     FVSFGGTSLMVTLFMIGVLLNISRQGITRTRTA"
     gene            34690..37230
                     /locus_tag="PKI64_00770"
     CDS             34690..37230
                     /locus_tag="PKI64_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOD99570.1"
                     /translation="MAGKKGKLTNTRREVTGVVLQGLWILLLLALASYDARDTSDWAS
                     AGHETLNYIGPVGAWAAWVVFRLFGLAGFVLPALLAVWGLLMIFQSGERVWPRALWML
                     GILCGVCVLMDIQLPFWNRISPERFNLNQMPGGLAGLFLGRMFLTQYLGVAGSAIISL
                     VVMISGLFFVSQTHPIELWRAIADAWHNWRERREERAAQNRTAQEQAEHEALKLERER
                     LKLERELERERRKQEMEDRRRQREREQEEREAARQREAAERAQREREAQEAREREEAA
                     RKAEFQKRLAEQQKAEAERRAAEERAAKQARVEAEKAAKRAAEEASLPPPLWTLPPMS
                     LLAPVPEGAGARAGADELDRTIRVIRGTLAEFGIDVEVTHVEQGPVVTRYELLPAANV
                     KLERISGLANNITLALKAESIRVQAPIPGKGVVGIEVPNRHAAPVVLRQLLESAAWTT
                     SKAALPLALGQDVSGHVLMADLASLPHLLIAGATGQGKTVCMNSILAGLLMTRSPEEL
                     RLILIDPKIVEFSGYNNLPHLLVPVITDAKKVVGGLRVAIQEMERRFKMFHRAKVRDI
                     RSFNQRPKAAQAALFDETELQNAQATEDGMPLPPPETIEETEEPLPERLPYWVIIIDE
                     LADLMLTAQQDIEPRIQKLTQLARATGIHMIIATQRPTVDIITGTIKSNIPGRVAFKV
                     AQKNDSRVVLDQEGADKLVGRGDMLVLTGANKIVRAQGAWTKDEEILAIAEHWKQQGA
                     PRFDQRLMGKEGKEGGTDLPDMPEEDEELLQQAMEVIRLTRRASTSSIQRRLRIGYTR
                     AARLMDILEEKGLVGPSRGAEAREILFDIDGLGQGANGADGGEDEPLA"
     gene            37240..42510
                     /locus_tag="PKI64_00775"
     CDS             37240..42510
                     /locus_tag="PKI64_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99571.1"
                     /translation="MSGIGSQLREAREHRGVTVGEAASATKLKVLVVDAMERDDYRRL
                     IAPTYAKGFYKLYCDYLGLDPEPFIQAYLHFSGESDDKTELIRDPKKKPGLFSGLQKK
                     MKDLQDRKEMQRKAKEIAEAQEKARQMGRQHVAAELAGAEPPAQQPALGQQELPLLDH
                     PQPTPEPPPAEEEPELIPEPEPEVEEAPAPEEPVLQVVAQPEPPPPPPPPAPAPERRT
                     RAVGKKKAPVSPREEAEERARIVEERRRQLQEALRQSEDELRRAHEEAERRAREEVER
                     KAQEAEQNRRQLEKELKKREAAERQAREEAEQRLREEAARRAEEEAKLLAREEAERQA
                     RLEAEQRIREIEEQRRQLEDELQRREEAARLEREAIERLWQEEENKRLQAELEQQARE
                     AAERQAREAAERKVAETEASRRQLEEELRQFEENARRAREAAEQRAREEAEQRAREEA
                     ERKAREEAERKAREKAERQVEATEAKRRQLEEELQRREVEARKAREEAERKAQEAEAQ
                     FAREKAEQEAREEAEREARLAVEQLVRETEERRRQLEAELKRREEETRLARIAAEEQA
                     QLEAQRRAEVEAELYARSEAERAAREAAEEKVAATEEQRRQLEEELHRRAEEAALAQA
                     AAEQRAREEAEHRAQVEAELQARGEAEREARELAEAKVAATEEQRRQLEEELRRREEE
                     ARLAAEEAERRAQEEAEHRAQVEAELQARGEAERVARELAEEKVRETEERRRALEAEL
                     QRHEEEARLAAEEAERRAQEEAEQRAQVEAELLAREEAERQAREAAEQQAQELEARRE
                     QLEAELHRQEEMARQEREKLESLLQEAAERRALMEAEHQQREEAERLAREEAEAQQRK
                     LEEAFQQREVEVRIAREEAVRLHEESNRQTREAAEHKVRTAREAAEQKVREIEERRLA
                     LEQELQQREEEAAAAREEAERRMQEEIERRAQVEAEWREREEAERNAREEVELQVQAA
                     GTRLAELEAELRQRELEAVAAREEAERNAREEAERRAQVEAEWQAREEAERAAREEIE
                     QKAQQTAIKLRSLEDELVAREEEAQQAREELERRIWEESDHRARVEQELLEREEAARV
                     AQESYEAAAARAADKIREMEADLARGEEEARQAQLELERRIQEESDHRARVEEELQAR
                     EAAARAAQEAYEADAARAADKIRDMEAELERREEAARLAREEAAERAREEAAQRARVE
                     AELQAQGEAERQAREAAELKVRVSEERLAELEQELTRRTEEVRQALANAEQQAREEAA
                     RRAQMEQDHKQRAAAEQAARAAAEKEVEKTEERRRKLEEQLRAKEREVVVAREAAEAR
                     AAEEAAFRAQQEAEQAARAAAEQEARAAAQQKIAEAEQRIAQLAAELQTREAEVRQAR
                     EEVVLRVNAEAEARTREESERQAREQAEKEERAAAEARFRAEEQRRLQLEEELLRREQ
                     EAQQVRAESAQKIAAEARAREEAEKELTARMAAEAKAREEAERKVQKAEELRRQFEEA
                     LRAKEREAAAAREEIERILAEAEERRQREASEWQSSEASIRQQHEQELARLQKEAEAR
                     VAAEFARREEERVALAKQEEALRLQREQASRAQEEFQAQLQRQQELEEANRAMAEALA
                     TQEQLRQELEKKLVEAERRRQELIARDRTARAQRAAQAQTTTPVIKTTPPPAPATVAQ
                     VSPAAPVSKKQRIPLRARWASLTAATRRGWQAIPWQVWRTAASLVVLVAVWVGAAHAC
                     ARIKARSASAPVVARPLVDSCRAAPEPYLD"
     gene            42516..43139
                     /gene="pgsA"
                     /locus_tag="PKI64_00780"
     CDS             42516..43139
                     /gene="pgsA"
                     /locus_tag="PKI64_00780"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOD99572.1"
                     /translation="MNLPNTLTVSRLIAAAVVMLAMALPIPFSTTLAFVVFVVASITD
                     YWDGRLARTHYGVTAFGKLMDPLADKVLVCAALVSFVGVRLKFEPNYSLVPAWVVVVI
                     IAREFAVTGLRLLAAATDQKRIISAGSWGKWKTAWQMTAIIATFILLAVREDLMPYLH
                     MSKTFLRQYDAAFVITSWVLSTLVVLVTVISGWKYFADHWDLVTAEM"
     gene            43173..43886
                     /locus_tag="PKI64_00785"
     CDS             43173..43886
                     /locus_tag="PKI64_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD99573.1"
                     /translation="MKQMWKWILSVAVLPAGTAWAQTEPAAGLTLADLMRMGGWLMYV
                     LGALSVLGLAMVVYYALVLRPRYIAPTAQALRLRELLKARRLSDARAVCSQQPTALAA
                     VTAAGLDFLKENPNGADAMLKEIMESEGARQADRLQNMIHYLVDLSAVAPMVGLLGTV
                     IGLLKAFNNVAFDLAKARPMELASGVGQALITTIAGLIIAIPAVMAYSWFRGRVIKLT
                     GQLEESATALLAVLEAKGE"
     gene            43887..44306
                     /locus_tag="PKI64_00790"
     CDS             43887..44306
                     /locus_tag="PKI64_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD99574.1"
                     /translation="MNFRKKLPTPGNLGVPLTPMIDVVFLLLCFFVTSQIFAQWETEI
                     DIALPTAATGDMPQRLPGEVIINVLADGTAVVNGQTLDDAALRAMMDRLVQLFPGQPV
                     LLRADKSTAYEHVVRVLDTCRQADIWNISFATLAPGS"
     gene            44306..46618
                     /locus_tag="PKI64_00795"
     CDS             44306..46618
                     /locus_tag="PKI64_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99575.1"
                     /translation="MKRLVPVLLLGIVLATRVWALSADEQMRFADGIYLRGFYETALT
                     EYIALIRDNPGSPHEVAALYRIGECYRQLGNQAGAERFYKRVAQEFSQSPQAPRAELR
                     RAEIAIADQQYAAAQAILTTLQTRDATPETAAAAGYYLGYSRLKNNDAPGADAAFRQV
                     LADFADSPYASYAALDLAALHAKDKTPATQMTAWYEQAVAAAPTPAAKAEALFRWGDW
                     AYRNEDYQQAADTLQALLLELPDQPRARDAWLAAGWSLYFLERTPEAMELAEKLAHDA
                     PDANLAAAGVYLRANCLRKLNRDGEALVDYETVVRDYPRTDYAARAAYEIMVTRFKRG
                     EYDKVLVVAPAQPAKEQAADVLWMRAEAERNLGRQDDARGHYEALVKEFPRATQAVPA
                     LLRLGEMARAAGRQSEAAAWFQRVAQDYPKNEAAAEALKAAALANLRAGDPKAALAGW
                     DDLLARSPTGTAAAEARLQKALVLIELKKPKDALALLDALVADAPAGASIAAAQYWRG
                     VLLAEQEKWSAAEAALRACLAAAPEPQTASLARLRLAVALQRLQRLDEAADQLAPLLA
                     QPDMVADNPALIEWLIRRSFEQGDDARALEAATALAQHAREASWRQIGWHWVGTSQAQ
                     LGHEKEAVASYRKAVAEPAHTREGTEAQLLLAELELKNGALEKAEAAFTAAGEAAAGE
                     DTLDLRARAYFGLAETAEAAGQFDKAARHFMSVAVLYDDPQWTPQSLYRASQLFGKLG
                     KKAEQESTRRELLQRYPDSSFARQLQEAAP"
     gene            46615..47133
                     /locus_tag="PKI64_00800"
     CDS             46615..47133
                     /locus_tag="PKI64_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99576.1"
                     /translation="MNSEWNIRACADQCMRCQRKFADKENLMSRLCFAPEGYLREDFC
                     GGCWPERATPTAAAEVSAWAAVWHAPAPKAPEPLKKETAESLLRELMETDDPAKRNVI
                     FILAVMLERRRILVEKEVQTPEGSGTKIRVYEHKQTGESFVVPDPQLRLSEIEHVQRE
                     VMELLGIAAPSS"
     gene            47130..47996
                     /locus_tag="PKI64_00805"
     CDS             47130..47996
                     /locus_tag="PKI64_00805"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOD99577.1"
                     /translation="MSPARPKHFMALIRRGAWLLALVSLPLVALAEKTPPGPPWDDPP
                     PELWFPVGEAIDYEVFWGVFMVGEATAQAQWREQDGRRLLALTVDAQSNGIVEKLYPV
                     KEHLETLLDPVTFLPISFEKESQEGRRYYHEITTFDHATGQGYWRSLRSGKEKEFAIA
                     DDTRDLLGLMYWIRKDPIRAGETREFPGVMTDEKLYEVIVEAGKAERVALSEYGKVQC
                     IKMEPKGKFNGMFVRKGRMWLWVSDDPRYTICRVAASVPVASIKIMLKRVRGPGDDFW
                     VQAGDNKSGRKK"
     gene            47993..49006
                     /locus_tag="PKI64_00810"
     CDS             47993..49006
                     /locus_tag="PKI64_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD99578.1"
                     /translation="MRISAKRAALWLRKFPRRRVLVVGDLMLDRYIYGAVSRISPEAP
                     VPVVRVTREASMPGGASNVAVNIRALGGKAAVAGMLGKDAAGVELRWLLTHAEVDVEC
                     AMLFAGASTIIKERIIAERQQVVRVDFERQVPWTDRQTEKFLAMLAVELARVDGVIIE
                     DYDKGVITQPVVDLVLQIAGKRGIPVGLDPKEGHQLDIQGITVATPNRKEAFGIAGLL
                     DPGAKENPLTDKALLKVGQILMEKWAAQNLVITLGAQGMFLMSRGKAPRHVPTRAREV
                     FDVSGAGDTVIATCVTALAAGADFLAAAELANIAAGVVVGKLGTASCSPQELLAHMKQ
                     PAT"
     gene            49003..49764
                     /gene="kdsB"
                     /locus_tag="PKI64_00815"
     CDS             49003..49764
                     /gene="kdsB"
                     /locus_tag="PKI64_00815"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOD99579.1"
                     /translation="MSARVVGVIPARWGSTRFPGKSLAMVGGKPLLAWVVERTKQARR
                     LAEILVATDDERIAAAARALDVRVAMTRADHPSGTDRIAEAIRELDAPHVINIQGDEP
                     LIDPALIDRIAATLADNSRWDMATAAAPVHSAKELHNPDVVKVVRDAAGRALYFSRAV
                     IPFVRGAAPDFAPVPGQHWRHIGIYGYQRAFLERLVREPPGALENLEKLEQLRALAIG
                     CRMAVLETDHVGIGVDSPADVPQAEALLRQAGLLR"
BASE COUNT         9818 a        14458 c        16483 g         9040 t
ORIGIN      
        1 ggcgatgatg gctaccgcat caaccggatc aacaacgtga attgtttcgt catcaatacg
       61 gctgcggatg attatcgctt cgccaatcac gatagcgggc tgcccatgcc catgcgtgag
      121 ttattcagcc gccgcgcgcg ggccggccgc caagatcaga tttccaccat cttcaccatg
      181 tgggaggagt tcagcaatct gtcgcgggcc gatgcctatg accgcaacct gcgctggctg
      241 gccaaccgcc cgtggattca actggtctcg ctggaggata ttgccgctgg ccggatcgcg
      301 ctgccttgga accagagttg ggagccgatt gaccgcggta acaccgccgg cgccaagcag
      361 tcgcacgact ggatcaacca cgccaacaac gaaaattacg acaactggta caatggcagt
      421 tggcgtcacg aaggactggt gggcaagaag tttgaacttc gtcccggcgt gactaatgcc
      481 accgcctacg gcatggtcta tacgggcgga atcatctcca acacctggac caaggtggcc
      541 ggtattacca atgccgacat caagcgcctt gcgcgcgaag tgctgcacgc ctcggtgttc
      601 gaaaccgctt tccataacga aaacaataac gatctgacca agtggagcat cggcggctat
      661 atcaatcccg ccagcgacaa ccaaaccctg atcggctttg ccaagcaggc ccagagtcaa
      721 actcgactgg cggccattta ttcgtgggca gattcatggg caacggttgc ccctggactg
      781 gcaaccactc agaccgccag cgtggatgtg gaccaggacg gcgaaaatga atacgtgctt
      841 tacaacaagc acgtggctgc ggtcttcgag cgaatcggcg ggcgcatgat tgcggcatgg
      901 caccgcgatg gcgatggccg catccggcag atgatcggca acctggcctc gtgcgccggc
      961 agcgagacgg aagtggaagg cgcttccaat gtggatacga acacgggtgc agtcgtggct
     1021 taccgtacgt cggcgctgaa ggactggtat gccggcaccg cgcaatacgt caacgacctc
     1081 tacaccgtca cgaccaacgg gacagatggc tggaagctga cttcgtccga tggcaagatt
     1141 tccaaaacgg tcactctggc tcccacggcc acaagctttg ccgtccaata caccatcaat
     1201 ccatcgctca atggcggcgt gctgtatgtc cgcaatggct tctcgcccaa tttgagcgag
     1261 ctgctgctgg ccggccagcg cgggtttgtg gataccatta gcgcgactgg cggcgtggtg
     1321 gcgctcacca cgacgaacaa ccactcgggc gtgctgctga cgatgactca aggtcaagtc
     1381 aataccgccg cgcgcgacga ccatcaaaac cacgagtacg attcggtccg aatgcggaat
     1441 caggcccaaa cccgccaagt ggaactcatg ggaaccaact cgatcgcgtt ctcgatcgac
     1501 ctttttgccg aagatgccaa caatgagccg ccggtgattg agtttacccc gcaggggccg
     1561 tacaccaacg cagtcggcac taccaacacc tttacagtca gcgtgaccga tcccgaggat
     1621 gatccggtca tactgaccgt gggtacgttg ccctttaccg ccaccttcaa tcctgaaacg
     1681 ggcgtgtttg cttggtgggt caccaacatg ggttccgccg gctccaccag cgtggtgacg
     1741 ttcgtggcgg atgacggcgt gaacgtggtc agcaattacg cgacgatcgt ggtgccgtgg
     1801 gatgccaacg gcaacgacat tcccgatgat tgggagttcc gctatttcaa cggcgacctc
     1861 tcgcaaacca aggatggcga ttacgacaac gacaagttct ccaactttgc cgaatggctg
     1921 gccagcaccg accccatggc agcgggcagc tatatcgggt gggaaatgca aatggtagtg
     1981 aacgacggca tgatgttgcg cttccagtcc gttgatggcc gtacatatca cattgaaggc
     2041 aatgatggcg atctgcccaa cgccgcggct tggcaccacc ttgccaccgt gtacggcacc
     2101 gggactcaaa ccgactgggt ggataacgct tatcccacca acgccgttcg ccactaccgg
     2161 atcaagatac cccgccacgt gccttgacct agcgtctgcg gccatagctg ttctcgtagc
     2221 tcttttgtta gccgggaacg gttgaggagg ggaaccttcc ctccgtgggg aacggctgga
     2281 aggtctgcgg ccgttccaat cctacagtgc accccggctg cggccatggc agttccggca
     2341 catttgatcc catgaaatag aggcggaacg ccattcgtca attcagttaa ctgccctcat
     2401 ccacgggcat attttagcga taaataagac aaattaatag aatcttaata ttcataatta
     2461 cgctattcat accatctggc ccactggcca caaagtgatt aaatacctgt aaatagcggt
     2521 gatcttcaca ttattcaccg ggaaaacacc gcaataaata catccatctt gatataaatc
     2581 actacgtaaa caccggtgcg atagtaaata gatatcatcg aaaattaatc ataaattaat
     2641 cgtatatcta ttgcgaaaac ctgcgttatg cagtattcta aggctgtgtt tggaaaagat
     2701 ggcggggtta ttcttaacat aatcctaata aataatctga tcggaaagat gaaaaagagc
     2761 agttcaaata aaacagacca gaccgtgata gtggcaaatt atgccgttat ccgcctgctg
     2821 gttgtggttg gactgttgct gggggcgacc atcgccgacg cgcaatggcg gataacgaac
     2881 tctcaactgc cggtgaagaa ggccacgcag aagattgatt tcccgcccat tccagaccat
     2941 atcacgacct cgaccgtagc gctggttgcc acggcttcga gtggcttgcc tgttgaattt
     3001 tctgttgagg aggggcccgc gcaattagct ggcaacgttt tgactttccg tggcgcgggc
     3061 aaagtgacag tgaaagccag ccagcccggg gacgagcact gggcgccggc caaagatgtt
     3121 tcccagagtt tcagggtgag cacggcagag gcaaagattg tcctgtcggc gttggagcag
     3181 gcctataacg gcagaccgcg cgtggtgcgt gtggagacaa tgccgcccga tctgagggtt
     3241 ctggtgactt atgacggcaa aaaagagccg ccgattgatg cgggcaagca cagcgttgcc
     3301 gtaatggttg tggaccccat gtggcatggc aaagccgagg gcttgctggt ggttcgaccg
     3361 gggaaacagg tcattgattt tccaccgatt cccgatcaga caacatcatc taccgtgctg
     3421 ctcagggcca aggcttccag cgggttgccg gtcgaattca tggtgctgga agggccggcc
     3481 aaattggaag ggaatctgtt gtatttcacc gaggcgggag tggtggtggt gaaagccagc
     3541 caaccgggaa ataacaactg ggctccggcc aatgatgtgc tgcagaagtt cgaggtgatc
     3601 aatgatcagg ctgtggtcat cctgtcgaat cttgatcagg tctatgatgg cacaccgcgc
     3661 gcggtacagg tggataccaa gccgcccgat ctgaaggtgc tcgtgaccta tcagggtgat
     3721 cccaaaccgc cgatcaatgc cggcgcctac aaggtgcggg cagtggtgaa ggatccattc
     3781 tggacaggcg aggcctttgg caccctgagc atcaagccgg cggagcagtt cattgatttt
     3841 ccgcaggtgc cggatcaagt ggcgacggca gttatcaggc tggaggccaa ggctagtagt
     3901 ggcttaccgg ttcagttttc ggtggcgtcc ggtccggcgg tatgggatgg ccggttcttg
     3961 agctttaccg gggcgggctc tgtgacggtt gtagccagtc agccgggcaa cggaaactgg
     4021 ctgccggcgc cgaaagttgc caaccgcttc gaggtgatca aggacaccgc cgggatcatg
     4081 ctgtatgatc tgcggcagct ttatgacggc acaccacggc aagtcggggt tagaaccaag
     4141 cccgccggtt tgccggtgaa agtgacctat aacgatgacc ccgccccacc ggtcaacgcc
     4201 ggcaagtact gggtagtcgc gatggttgcg gatccgcgct gggaaggccg ggccgaggat
     4261 gtatttttta ttgatccggc caagcagacg attgattttc cggagattcc ccctcaactg
     4321 gcttcggcga ccatcgagtt ggaggccaag gccagtagtg gtctgccggt cgagtttgcg
     4381 gttgactcgg gacccgggca attggtgggt aatctgttga gtttcaccgg tgcggggcct
     4441 gtcacgatca aggcgatgca gccgggggat aacaactggc tgccggctga atcggttggt
     4501 caaaccatca aagtgatccc ggatcaagcc gcggttacct tgtcgaacct tacacaggtc
     4561 tatgacggta cgccgcgtgt tgtgagcgtg actacggatc cgcccggtct ggcggtggaa
     4621 gtgacctatg acggttcact gaacgtaccg gtcaacgcgg gcacctatca ggttgtggcc
     4681 acagtagtgg atggccgata tgaggggcag gcgcgggacg ttttgacggt tgaaccggca
     4741 aaagtgacta ttgtgctgga tgacttggag caggtgtaca acggcgaacc tagggcggtc
     4801 actgttttca tcgagccgga aggcctgcct ttggagattc tctacagcca gttggggctc
     4861 gtcatcttgg gcaaggaact accctcggaa gatcctcctg tggacgcggg cgattatctg
     4921 gtttcggtaa cagttgttga tgacaactac gagggtcgtg ttgaggacaa gctgaccatt
     4981 ctgaaggcct ctcaagtaat tgattttgcc ccgattgccg actgttggac aacggacatt
     5041 gtcgaactcg atgcaaccgc caccagtggt ctgccggtga gctttgaggt ggtgtcgggg
     5101 cccgccttgt tggaaggcaa cattctgacc tttaccagtg cgggcactgt ggaggtacaa
     5161 gccgctcagg agggcaacgg caattggaat gaggccgagc ccgtcgccca tagcttcgag
     5221 gtcaaggcct cgaaccatga gcaggaaggt ccctatacct atattgttga ggacgacgaa
     5281 gcgacaatcg tcgggttcga tgaaagctat cagggcactc tggctttgcc tggcagtttg
     5341 ggcgggtttc cacttgtcgg cattgacgcc ggggcgtttg ccaatagcag cggactgacg
     5401 agtatcttta ttccgggcaa catcacctgg cttggtgaag acgctttcac aggctgcacc
     5461 aacctcgagc ggatttactt tctgagcgat gtcctcgctg gggaacctgc cagcttgggg
     5521 gccagccggg ccacagctta ttatctgccc gggtccgaag gctggggtgc cacctttggc
     5581 ggctgcccag cggttctctg gaatccgaag tttgtaaacg gctattgctc accgttggcc
     5641 gaaaactgcc aggtggtggg cgcgcccaat ctgcccattg gcattcagag ttccaccaac
     5701 ctgcttgcgg gcgagtggat gtgggaagtc accctgacca acctgaccgc agaaggcacg
     5761 ttctgttttg ccaagtatct cgcggatgtt gaggaagagc gctactaccg catcatagcg
     5821 ccttgatcgc cccggcaacg gggagggggg ggaaagctcg tgaattcaac cgccaaggtt
     5881 gttctcttga ggtttttgtt ggcgggcatg attggggaac gaaatctccc cgccggcaaa
     5941 ggttggaaag tttgcgactg ttcctgctct acacaattgc cttagcgatt tgggcggcca
     6001 cgcgggcgtt gtttttgagc agggcgatat tggcggcacg actgcggtcg ccggtgagtt
     6061 cgctgacgcg tgccaacagg aacggcgtgg tctccttgcc gcgaatgcct tgggcatccg
     6121 cttcctgcaa ggcctgctga atcgcggctt cggcaatttc tgccggtagt tcatccgccg
     6181 ccgggatggg gttggttatc agcatgccgg tgcggaagcc cagttgctct cgcgcacgga
     6241 tcatggctgc gatttcctcc acggtatcgc aacgcacgtc caatggcagg ccggaactgc
     6301 gcgaataaaa tgccgggaac tctgcgcacc cgtagccgac caccggcacg ccgtgggttt
     6361 ccagcacctc cagcgtcaat ggcaaatcca gaatggcctt agcgcccgcg caaaccactg
     6421 ccacgggagt ttgcgccagc tccagcaggt cggcactgac atcaacagga tgaccccggt
     6481 gaaccccacc gatacctcca gtggcaaaaa ctttaattcc ggccaagtgc gccacaatca
     6541 tggtacctgc cacggtagtt gcaccatcgc ccttttgggc cagcacgatt gggaaatcgc
     6601 gccggctaca tttgcgtacg ttagtggctt gggccagata cgtcagttgt tcactcgtga
     6661 gccccacggt gatttggcct cgcagcacgg caatcgtggc tggggtggcg ccgttatcgc
     6721 gcacagcctg ttccatggcc tgagccactt ccagattgat ggggtggggc agtccgtggg
     6781 tgataagggt gctttccagc gcgacgaccg gtcgtccggc ggcaagagcg tttgcaacat
     6841 ccggatgaaa tttcataagt tcagcaatca tggaagcctt tcgttcgcgt ttcactatgc
     6901 gagcgcgcca cccttggttc aagcctaatg cgcaacatca ggtactacga tataatgtct
     6961 tcgtggcgag caggttttgc cggttagtac cattcggcgt tagttcatat attgcaataa
     7021 gtgaattcac atattgcaat aagtgaagca agtatggggt gcgtttttcg gcggttgtat
     7081 ctggcgtaaa gcgtttttgt tggcaggcgt ttgcggtatt gcgtgggcca ttggaaggag
     7141 tagatttctt gcaacaacaa accacctacc caaggaactc ctatatgaaa tctaccgtgc
     7201 gcaattggct catggttttt ttgacgactg gtctcttcgc cttgacggct gccgcgcagc
     7261 cgatttacct caacgccggg gtcattgata cggcgacaac cgagccgcgc aaagcggtgg
     7321 cgggaacaac aggcgaccag ttgcacttgg tgcagtttga cggtccgatt caaccggaat
     7381 gggtggcgca actggaggcg gcgggatggc gcatcgtgga ttttgtgccg gacaacacct
     7441 acatcgtgta tggcggccag accgcccgca aaaagctgcg cgcgcaggtg tcgcatgtgc
     7501 agtgggaagg cgcgtttttg ccgagcgaca agattcagcc gcgcgccacc ccggcggcat
     7561 tgaaacaacg caagctgacc aagccggaca gcaagttgtt tgctgtgcag ttggtggaag
     7621 acgcggcggc caatgccgcg acgattgcct ggctggaaga agcggcagga gaaccgctcg
     7681 cgagcattgc gacaaatgaa actttgaggt ttgtaaatat tcgcgtggcg ctgccagaga
     7741 atcgtattga agaattggca gggcgtcagg atgtgctggt cattgacgtc tatgaacctc
     7801 cgcagcgccg tggcgagcgg caggggcaga ttgtggcagg caatgtgaat gcggcgggaa
     7861 gccagccgac ggggccgggc tatctgtcgt ggatcacaag caagggattc acacaggccc
     7921 agtttgatgc atcagggatg gtcattgaca tcgcagacga cggctgggat cgtggcgtgg
     7981 cagcgaatcc ggcaaaccgt gagtttcgcg tggatggcga tgcagccaag agcagccgcg
     8041 tgaagtattc gcgcacggca tccaaactga cggaagcgaa ctcggcgggt acggatggcc
     8101 acggcaacat caacatcagc attatcggcg gctacaacaa ttcatcgggc aatccgtaca
     8161 gtgacgccga cggttatcat cgcgggttgg gcatcaatcc gttcgcgttg atgggcaaca
     8221 cgaaggtatt tgcagatggt ggtgattggg accccagcga ggcgcaggaa aaaatattca
     8281 ttgcaacaaa ctatcacagc ggggttcgca tcagttcgga cagttgggga ttggatggcg
     8341 atggcgcgta caacgtggat gcccagaatt acgacacgtg gacgcgcgac tcgcagccgg
     8401 gtgtgagcgg gaaccagcaa gtgctttacg tatttgcggc gggcaatgat gggccaggca
     8461 gcaaaacaat cggtgcgccg gggacgggca agaatatttt ctccatcggg gcgtcggaaa
     8521 actacaatat gtttgggaca gatggctgcg ccgtgggtaa taccggtgcg aataatgcca
     8581 atgacatcat tgatttctcc agccgcggac cttgcaagga cagccgtaag aagccggaca
     8641 tcgtggcccc ggggacgcat gtagcgggtg cggcaagctt tgccaacggc tatacgggcg
     8701 aaggagtgtg cgacaagtac cagcccgccg gccaaacgaa ctatgcagca tcaagcggaa
     8761 ccagccactc gacgccggcg gtcgcgggcg gagcatccct tgtctaccag tatttcatca
     8821 accaggggtg gactattccc agcccggcga tgatgaaggc gtatctgatg aactccacac
     8881 gatatctgac aggcgtggat gccaacgata cgctgccctc caacaatcaa ggcatggggt
     8941 tgatgaatct gggcacggcg tttgatggta cacagcgcat gctgcgcgac caattgacca
     9001 acgatatttt cacggcgagt ggccaaacgc ggactttctt tgcgaacgtg gtggagacga
     9061 acaagccgct acggatcacg ttgggctgga cggacgcgcc gggatcgctg tccggcaacg
     9121 catataaaaa caacttggat ctcaccgtgc tggtgggcgg ccagacgtat ctggggaact
     9181 cgtttgccgg tgccttcacg acaccgggcg gcaccgcaga cccgcgcaac aacgtggaaa
     9241 gcgtattttt gccggcgggg cagagcggcc tgatcaccat caaggtgacc gcggccaaca
     9301 tcaactccga cggcgtgccg ggttatggcg gagcgctgga tcaagacttt gtgctggtgg
     9361 cctacaatgc cgaagaatac atccccagca actatccgcc ggtgttggag cccattggca
     9421 acaaggccat ggccaccaat cgcctgatgc agtttactgt gacggcgagc gacccggtgg
     9481 atggcgacag tgtgcgcctc tgggcgacag gcatcccggc atgggccaca tttgccggcg
     9541 ccacgaatgc cggtaccgcc agcgcgcaat tcagcggcac tacgccggaa accacgggca
     9601 cgtacaatgt ggcattctat gccgccgaca aagatggcac aaacaccgag agcattgtga
     9661 ttgtcgtcaa cgacatcaac tgcgtgccag ccgtgctgct gtctgaagac ttcgacgact
     9721 ccaccggtgt ccccaccggc tgggtgaata gtgggtcagc caatgacacg tcggccacgc
     9781 actacaagag cagtccgaac tgccggggat ttggtgccgg cgcgtcgctg gaaaccccac
     9841 caatcaacac gccgacacag attgtatttt atgtggacgc cagcggcaat ggcgcgggca
     9901 aaaccgccac gctggactac aagatcggca gcggcgaatg gaagaaggtg ggtaacttca
     9961 ttgccaccac cgcgggcaag acggagacct ttgatttgac cagttcacct gatttgcgcg
    10021 agcaggccaa cgtcagcttc cggttcagtt cgacattcag cacttggtat ctggacgatg
    10081 taatcatttc ggggatggat tgcggcggtg gcttcctgcc caacaacccg ccgacgattg
    10141 gcgtggaagg ggtgggcggc acaaacaagg ccgggagcgt tggcgtggag ttgtcgttca
    10201 cggtgacggc gaatgatgcc gacagcgata acgtctcgct gcaagccacc tcgttgccgg
    10261 ctggggcgac atttgccacc gccagtggac cagcgccagt gcaaagcact ttcacgtgga
    10321 cccccggcag caccggcaag tattcggctg tgttcacggc caacgatggc aaggccacgg
    10381 ctacccaagc ggtgtccatc acggtcagcg cgtttgtggc tccgctgttg gcgccggaga
    10441 tcttggcgcc cagcgctgtc ttggccaacc aattcaacgc caactggtcg gcggtgagca
    10501 atgccaccgg ctaccgtttg gatgtgtccg actcgccgag cttctccggc ggcggcggcg
    10561 ggctggttct ggcggaggat tttgctgggg tcacggtatc caacgccaac gacatcagcg
    10621 cgagcttgga cacctatacg ctcgtccccg gatggaccgg cgccaagatt tatccggaga
    10681 tggggtggat caaactcggc tcggcttcgg ccaagggcta catcacgacg ccaacgcttg
    10741 acttatcggg gaatggcggc gtggccaccc tgacctttga attggcgaaa tacgggacgg
    10801 atgccacgac ggtgcaagtt ttgcatgctc cggatggcag caattttaca caggtcggca
    10861 gtgatttgac tgccccgagc tcaaagaccc tgcagaccat tcagatcaac gatggcacgg
    10921 ccaattccaa gattcagatc tcggccaagg gcctatccaa gagccggttc tatttggaca
    10981 acattcaggt ggaaggtggc ggcggtgccg gcaactttgt tccggggtat cagaatcgcg
    11041 acgtcggcaa cgtcaccacg tatgcggtga ccgggctggc cgaaaacgtg acctattact
    11101 atcgcgcacg ggcatacgat gcctcgcgga acagcgatta ttccgctacg accaacgtca
    11161 cgaccactgc cggtgtagac atcccgccgg tcttgaaccc catcggcaac aagacggtcg
    11221 ccttgggtgg cagcttgcag tttgcgatca ccgccgtgcc gactgatggc gatgaagtga
    11281 ctttgacgac cagcgcgctg cctgctggcg ccaccttcgg ttccacgaac gaaaacggca
    11341 ccttccaatg gaacaacgct tcacccaccg gggtctatag cgtgactttc tatgcggaag
    11401 atgtggacgg tcaagacagc gaaaccatca cgatcactgt ctatgatccc gaagccgaat
    11461 tgcaggcgcc cgtcgtgcaa gctgcgagca gcgtgcaggc gcagcagttc aacgctaact
    11521 ggcttgcctc tgttggcgcc gtcggctacc gcttggatgt cgccaccgat gatagcttta
    11581 cggcaggtgg cggtgggggc ggaggtggtg ctgtcctgtc agaagacttt gcgggtttta
    11641 cggccgttga cggcactcaa gaccgctcgg gcagcctgaa cacttatctg cagacaccgg
    11701 gctggaccgg ttccaaggtg tacgaggacg cgggacgcgc caaactcggt agcagttcag
    11761 cactgggtta cattgtcacg ccaacggttg atctgtccgg caatggcggc aatgccacct
    11821 tgacctttga tctgggccag tacggcaccg atgtcggttc gattcaggtt cagcatgcgc
    11881 cggacggctc aaccttcagc ccaatcggct ctgacctgac ccctccggcg agtatgacgt
    11941 cacaaagtat tcccatcaca ggtggcacgg cgaattccaa aataaaattt actgccaaag
    12001 ttgcttccaa gaaccggttc cggctggaca acgtggaaat cacggcaggt tcgggtggcg
    12061 gcggtggctc gggcagtttc gtcgcgggct atgaagaccg cgatgtcggc aatgttacca
    12121 ctttcgcggt gaccggactg acggaaagca tcacatatta ctatcgcgtt cgcgcgtacg
    12181 atgcttcgac gaatagcggg tattccgcca cgaccaacgt cacgactgtc gcggctccgg
    12241 cgctgcagcc gccggtcatt caagcggcca gcggcgtgca agccgagcaa ttcaacgcca
    12301 actggctggc ttccgtcggc gccaccggct accggctgga tgttggtacc aatgaaacct
    12361 ttagcggcgg cggtggaggg ccgagcgaac ggctgctgct ggcgaccaac gcggcatcgt
    12421 ctgcgtccat ccaaactgat ggctggagcg ccaccgacac cggcggcagt acctacgtgc
    12481 agatggctaa gcccaccgcc acaatcctct cgcccgcctt ctccacggtg gggttcacca
    12541 atctgactgt ggacatgatg gcgcgaacct ataatggggt gaatgccacc tacaacatca
    12601 tcaccgtgtc cgtctccacc aacaatggca cggcgtggga taccctgggg accgtgctgc
    12661 ccctcagcag tactatggcg aacatgggca cgttgaccga tgcgactcac atcggccacg
    12721 ctgaaacccg tctgcgttgg cagacgcttg gcgcgaacgg caccattggc gcgggcgttc
    12781 agcagctcca catccgcggc tggacggctg gtggtggagt agcggccgct tatgtgcccg
    12841 gctacgagaa ccgcgacgta ggcaacgtga ccacgtttgc ggtgacgggg ttggtggaag
    12901 gcgtgacgta ctactatcgg gtgcgggcat acaacgcgag caccaccacg gtccattccg
    12961 cggtgaccag cgtggtgacg agcgccagca cgccgacgca gccggagttc gatacgttta
    13021 ccttcccgcc caatgcgacg ccggcggcga cgtttgcgtc gcaagcgggc atcacctatc
    13081 aactgcaata caccacggac ctgttggcga acccgccggt gtggactgcg gcggatacgg
    13141 aggtgggcac tggcgggaat gtcacgttgg aagacaccaa cgcggtggat tcgcggcgtt
    13201 attaccgcat tgtgatccca taagcgggcg acatcccgct gaagaagcgg cccggtcgcg
    13261 ttgatacgcg gccgggccgt tttccatgca gcagaccggg tggctttatc cgcgcaagtt
    13321 gtctgctttg atgagcttgc cgttggcaat cacgtgcaat ggcgccttgt gccagcgaaa
    13381 agcctcgcac accgtcttgg catccaaaat gaccaggctg gcgttgttct tttccttcaa
    13441 ctcgaacttc cgcaggttga tgcacttggc ggggttgaca gtcaccaggt cgtagagggt
    13501 ttccatttcc ggataggtgg tcatccagag caggtgggag gccaggaagg cgacttcgag
    13561 catgttgttg cggccgtagg ggtagtaggc atcggagatg tcgtcctgcc cgaggcacac
    13621 gaggttgcct tcttccagca gctccttcac gcgggcgtgg agggggccgg tatggggatc
    13681 gctcaccacg cccatgccgg cttggcggag caaggcggcg accttcttga agtacggggt
    13741 aggatagagg ctcatggcgc gggcgtggtg ggccaagttg cggcccacgc ggccttcctc
    13801 gatggtgcgc acggcgagca tttccagcgt cttgagggtg gggtccccgg catcatccac
    13861 gagcatggaa atatccgtat cgtaggcgat ggccagcttc atcatttcat caatgtggat
    13921 ttgttcatct ttttcggtga actcgatcca agggatgccg ccgacgacat cggcgccgag
    13981 gtccatggcc tccttcatga ggtccacggt gccgggttcg cgcaccacgc catcctgcgg
    14041 gaaagcgcag acctgcacat ccacgatgcc cttgaattca tcgcgggcct tgagcagcgc
    14101 cttgaggccg atgagcttgg ccttggaatc cacgtcggca aacgcgcgga tatgcaggtt
    14161 gccgtggagc gcggccagct tgagggcctt gcggacgttg gggaggatcc atttttcgtt
    14221 gtagttctcc ttgacgcgtg cggcgagttc aatggccgtc atggcgccgc ccatgtcggc
    14281 gccgtgatag gcgcgcaggg cgagagaatc catcatttcc agcgtataga ccttgcagag
    14341 gtgcaggtgg gtgttgacga acggttcggt gacgaggtta ccgccggcat caatgacttt
    14401 gcccgccttg ccgcggatgg gctgcttggt gattttctgg atgacgcctt tttcgatggc
    14461 gatattccag agtccagcct tgcggcgaag ctgcgcattt ttaatgagga tgtcgtacat
    14521 ggcggggcct ttctacggtt gcgggttcca gtctacacgt tacacattca cgggggcggc
    14581 ggcatcgccg caggcagggc attgcgggtt gcggcgggcg cgcacggcac gaaattcgac
    14641 ttcgaggccg tcgtaggtca ggagggtgtc ggtgagcgta ttgccgatgc gggcaagcac
    14701 tttgatggct tcgaccgctt gcagggagcc gatgacgccg gggatggcgc cgagggggcc
    14761 ggcggtgtgg gccgcggcgg gatcatccac cggcggcggg gtggggtgga tgcagcgcag
    14821 gcaggcggtc tggccggggc agactgtgag cacttggccg caaaaaccgg aaatgctgcc
    14881 gtggatgaag ttctttttca gcgccacgca cgtgtcggaa atcagatatt tggccgcgaa
    14941 attatcggta cagtccagga tcagctcgta gggagcaagc agtgcgcgcg cattttccgc
    15001 ggtgaggtac tcggcgtggg cggtgaagcg tacatgagga ttcagcgcgc gcagccgggt
    15061 gatggcggaa tccaccttgg ggcggccaat atccggcgta gcgtgcagga tttggcgctg
    15121 gaggttggag atttcgacca agtcggggtc catgaggccg atgttgccga cgccggcggc
    15181 ggcgagatag tagagcgcgg gcgaaccaag gccgccgcaa cccaccacca gcacgcggcc
    15241 ggcgcgcagg cggagttggg cagcctcgcc gaagccgggc aggtcgaggt ggcgggcgta
    15301 gcgctggcgt tcttcgtggg agaaagacat ggcggcgtta tttcagcggg aggaagggcg
    15361 tggcggaatc gccggaggac aacgccacaa acaccacgcc gacatacagc agcaacagca
    15421 ggacggcgag gaaggccccg atgatataca tccagcccca gcagcagaaa aaggtgcgca
    15481 gcttggagaa aaacggcagc atgtccggcg cgatgaacgg cgcgcggtcc agtgcggcgc
    15541 gcgcacccag cagggcgatg cccgccacca gcatggggat gccgaacagc atcacggaag
    15601 gcagcagcgc gctgaggatc atcatgatgg cgataaacgt catccacccg cgcaggccat
    15661 ccggcagggg caccttgccc tcgctgccgg cgggcgcttt caatgcggca aaagcctggc
    15721 tggcgggaat ccagttggcc aagccgtcat gccaaaccaa ggtgtggggg ggcagttcgc
    15781 cggcaataaa cttggcgcgc acgtgcgctt cctcgttggg gccgactttt tcgcgggtct
    15841 tcgggttgac aaaataccaa ttcgccatgg cactctccgg aagaaacacc atacgacagg
    15901 agccggggca aagggaagtc cagattcgcc gcggcggaac agccgaggcg cgcgagacta
    15961 ggagcggatt tggccggtac cgtggatggt gtatttgtag gtggtcagct cttccagcgc
    16021 catggggccg cgggcatgga gcttatcggt ggaaatgccg atttcggcgc ccatgccgaa
    16081 ctcgccgcca tcggtgaagc gggtggaggc attggcatag acgcaggcgg aatccacctc
    16141 ggcaaagaac ttgtcctggg ccttggtatc ctcggccaag atggcatcgg agtggtgcga
    16201 gccgtggcga ttgatgtgtg caatggcggc ttccagtgtg ggcacgacgc cgatagtcat
    16261 gacgagatcg agatattctt catcccaatc agcgggcgcg gcgggagcga gggcggggac
    16321 aatggcgcga gaggcttcgt ccgcgcgcag ctccacgcgc ttgtcggtca ggcgggcagc
    16381 cagcttgggg agaaactcgg ccgcgatttt ctcatgcacc agcacttttt ccagggcgtt
    16441 gcaaacgccg gggcggctgc atttggagtt ttcgatgatg tccaaggcct tggcctggtc
    16501 ggcgctttca tccacgaacg cgtagcacac gcccttgtaa tgcttgatga ccggcacgtg
    16561 ggcttgctcg gtgacggcgc ggatcagttt ttcgccacca cgggggatca cgaggtccac
    16621 gcgaccctgc aactggacca gttcgcggat ggcgtcatag tcggtggtgg tgatcaattg
    16681 aatggcattg gccggtaatc ccttgcgggt gccaccggct tgcagggtat ccaccagcgc
    16741 cttgttggat tctagcgcct ctttgccgcc gcgcaaaatg acggcgttgg aggttttgaa
    16801 gcacagcgcc gcggaatcca ccgtgacgtt ggggcgcgac tcaaaaatga tgccgatgac
    16861 gccgatgggc gtgcggattt tttggatgac gagaccgttg gggcgaatcc agcgcgaaat
    16921 ccgttgacca acggggtctt tgaggccgat aagcgcgcgt acgccgctaa tcatggaatc
    16981 catgcgcttg tcggtgagca gcaggcggtc gagcatggca tcggagagcc cggcctcacg
    17041 cccggcggcc atatcgcgcg cgttggcctc cttgatgcgg tcgcggttgg cttccagcga
    17101 ttccgccatg gcctccagaa tgatattctt gcggcgcgag ccgagggtgg ccagttggcg
    17161 ggcggccgtc acggcctgat cgcccatttg cagcatggtg tcgtgcagat tcatcggttt
    17221 tctcccatcc atcagcgcag catagttcag cgggccggcg gtgtcaaaag cacgaggttg
    17281 tcgcggtgga tgacctcgtc gggtgcgtcg gcgccgagca gctcgcggat ttttccactt
    17341 ttgaggccct tgagcagttg ggtttcggtg ctggaaaatt cggtgagccc ctgcgcgatg
    17401 caacggccgg cggcatccac gatttcgatg agatcgccgg catcgaagtc gccagtgacc
    17461 gcgcgaatgc cgaccggcag gaggctcttg ccgcgcagga ggagcgcgtc gcgcgcgcca
    17521 tcatccacgg tgaccttgcc ttgggcatga tggaaaaacg caacccaccg gcggcgcagg
    17581 ttcaggccgc cattggcggt gggcgcgggg gcggggggga tcaatgtgcc aacgttggcg
    17641 cccgcgacca cctgctgcag aatgtcatcc ttgcggccat tggcaattac gacggcggtg
    17701 cccatgcgcg cggcctcggc ggcggaggtc aacttgctgg ccatgccgcc agtggaaaaa
    17761 atactgcctt tgccctgcgt gtggctgagc gtgtcggcgg tgacatccgc caaataaggc
    17821 acgcgacggc tgcgcccctt ggcatcgggc gcgtggaagc cgttgacggt ggtcagcagc
    17881 acgagcaaat cggcctcgac cagcatggcc gagcgggagg cgagcgcgtc gttgtcgccg
    17941 aacttgattt catccacgga caccacgtcg ttttcattga cgatgggaat aatgccgtgc
    18001 tcgagcagct tgagcatggt gttgcgcgca ttgaggtgac ggcggcggtg ttcgaggtcg
    18061 tcgtgcgtca acagcacttg gccgatggtg catttttgac gggtgaacaa gcggtcatag
    18121 gccgccatca ggcgcgattg gccgacggct gccgccattt gcagcgtggg caggtcggcg
    18181 ggctttttct ttatgcccag cgcttgcagg ccggaggcaa tcgcgccgga cgacaccaca
    18241 atgacttcgc gcccggcgcg gcgcagcgcg gcgatatcct tcaccagcgc agtcatacgc
    18301 cgggtatcgg ggcggccatt gcgctgcacg agcacacggc tgccgatctt cacgaccacg
    18361 cggcgggaag cggccaaggc ttttctggcg gtttgatgag gcatgacggc ggcactgtgc
    18421 caaaagctcg cccgcttgaa aagagaggaa tggccggggg cgtgcgcttg caccgggcgc
    18481 gtgttgtccg attgacagcg gcggtggggc gggggaaagt aggcgcgtga atccagaacc
    18541 cgcatatcgc tatcttttcg ggccggtgcc gtcgcggcgg ctggggcgct cgttgggcgt
    18601 ggatttgaat ccgctgaaag tctgcacgga aaactgcatt ttttgccaga tcggccgcac
    18661 cacggagctg accgccgcgc gccgggagtg ggtgccgaca gcggacgtgc tcgcggaatt
    18721 cgatcgctgg caagcggccg ggggcactgc cgacttcatt acgctctcgg gatcgggcga
    18781 gcccaccatg catacggggt ttggcgaggt actggcccat gtgcgggccg cccgccagtt
    18841 caaaacggcg ctcctgagca acgggtcgct gctgtggctg ccggaagtgc ggcaggctgc
    18901 ggtgctggcc gatgtggtga aagtgacgct aagcgcgtgg gatgaggaat cgtttgcgcg
    18961 gattcaccgg cccgccgcgg gattgacgct ggcgcggttg ctggcgggag cccgcgcgct
    19021 gcgcgcggaa tacggcggcc aactgtgggt ggaggtcatg ctgttggcgc acatcaacga
    19081 cgcgcccgag caggtgcggg ccattgccga actggcgcgc acgctggcgc cagatgccat
    19141 ccacctcaac accgtgacgc gcccggcgca ggcgggggtg gcggtcagcc gggtcggcgc
    19201 ggcgtggctg cacacggtgg cgccatggtt tcaaccggtg gcggaagtgc cggagttcac
    19261 cggccaaata cccgcgccgg agcagatgac ggacgcggag tgggtggaat cgctggcgcg
    19321 gcatccactg gatctgtcgg ctctggccgc ggcggcgggg gtgccggtgg aagccgtgga
    19381 acagcgtttg cgcggtttgg tggccgcggg ccgcgtggtg attgaagagc gcgagggcca
    19441 gcgcgtcgcg cgcgtggcaa cgccatgaac ctgacgcggc agcaagtccg ggtgcgctac
    19501 accggcgatg tgcagggcgt gggctttcgc tatacggtgc gcaagctggc cacgccgctg
    19561 ggcgtgaccg gcggcgtgga aaatctgctg gatggttcgg tgcagatggt tgcggaagca
    19621 acgccggata cgctgcgcct gctgttgacc caaatccacg cgtcgcggct cggggcgcac
    19681 attgctgatg aacaaatcgc gtgggggccg gcgcaggggt tggccggctt ttattatcgg
    19741 tagtccacaa cccctcggaa ggcgtgttcc gccgggccag tcagggtgag gggagattcc
    19801 gccacttcga gaacatcacc gctggccgtc tgaacctgca cgggaaaact ggccagcccg
    19861 acgcgcgcgg cgatgagcgc cgccgccgtg atgcccgtgc cgcaggccag cgtttccgcc
    19921 tcgaccccgc gctcgtatgt acggatggcc aggcggtgcg aatccagcac gcggataaaa
    19981 ttgacgttgg tccccgccgg cgcaaacagc tcatgccgcc ggataaatgc gccaagctgg
    20041 ggaatatcca ccgcggcgag gtcggccacg ggcagcacgg cgtgcggcac gcccgtattg
    20101 acaaaatgca gcggccacgc ggcgccgttc cactccacgg acaaattgat ccgccaatcc
    20161 ttgggtggtg gcagatgaat gcgcaccaat ggggaaagga tttccgcgcg gatgatgccg
    20221 gccggtgatt tcatgtgcat ggccgcaggg gcaatgccca gctcgtgggc gaggcgggcc
    20281 acgcagcgcg cgccattgcc gcagaggtca gcctcgccgc cgtccggatt gaaaaaccgc
    20341 atccggaagt cggcgccggt tgccggttga atcagcaaca ggccatcgga gccgatgccg
    20401 cggcgtcggt cgcaaagctg cgcgatgaaa gcccggtcgg tagccggaaa agccagcgcg
    20461 cggtcatcca gcaggatgaa atcattgccg gcgccgtgca ttttccaaaa cgaccattgc
    20521 atggcatcac gatgccacgg gcggcgcgca aacacaagtc gcgccgcgcg cgcggccata
    20581 aaacaaagtt gcccgccggg gggcgcgttt gctacaaacg ccattcatga aaatcctttt
    20641 gacaggcggc tcgggctaca ttggcagcgt gaccacggaa ttgttgtgcg atgacggcca
    20701 cgaggtggtt gtttttgaca acctggagcg cggccaccgg gaggccgtgg acccgcgggc
    20761 caagctgatg gtgggcgatt tgcgcgaccc gcagcccatc cgcgaggcca tgcggcaggc
    20821 caagccggat gccgtcgtcc attttgccgc ctacgcgttg gtgggcgagt ccatgcagga
    20881 cccgcacatg tattttgaaa acaaccttgg cggcggcctg aacctgctgg ccgccatgct
    20941 ggagagcggc gtaaagaaaa ttattttttc ttcgacgtgc gccacctacg gccaaccaga
    21001 agtgatgccc atgaccgagg cgctgccgca aaagccaacc aatccctatg gcgaatccaa
    21061 gctgatgttt gaaactgcgc tgcgctgggc gcaggaacgc aacggcatcc agccggtttt
    21121 tctgcgctat ttcaacgcgt gcggcgccac ggaaaaatat ggcgaggatc acgatcccga
    21181 aactcacctg attcctaatg tgctgaaggt ggcgctgggg caggcccccg ccgtgccggt
    21241 gtttggcaac gattatccca cccccgacgg cacctgcatc cgggactata ttcatattgt
    21301 ggaccttgcc cgcgcccata ttctggcact ggaaaaagaa gtgaccggcg cgttcaacct
    21361 tggcaccggc actggttttt ccgtgcagca ggtggtggaa acatgccagc aggtgaccgg
    21421 ccacgagatt cctgtggcgc tttcaccgcg gcggccgggc gacccggcgt gcctcgtggc
    21481 cggcgccacc aaggcccatg aggtgctggg ctggcagccg cgctatcccg acttgcaaac
    21541 cattgtgcaa cacgcgtgga attggcacca cgcgcatccc cacggatatc gcaaggagtg
    21601 agatgagccg cttgccagta caaaccgaag gatttcgcgt ggataccagc cggccgcgca
    21661 gccggaccaa gatgcgggcc aagttccgca cggtgctcgg cgtcagcgcc ggagtgctgc
    21721 cgctgatggc gctggtaatt ttcctgatgt cctttttgca gattcgcgaa cagcgggaac
    21781 ccctgcacct cctcgctctg atcctgctgg gcagcggcat ggttgtgctg atgttctatg
    21841 cgtgggcgcg cgccgaaaaa ctgcgccgcc gcgaaatctc ggcgcgcaac cgcgaggcgc
    21901 gctatcgcga gcaggcggag catctgcgcc aggagcgcga gaaaaaagcg gcggaacgct
    21961 cggccggaaa tgcccaggtg agctgatgcc gcgccacccg gcagcccgca cgggcatggc
    22021 gctgattctg gtgctggcgg ccatcgtgct ggccgggggc ttggcgctct acctgcaagc
    22081 gcgcgcagcc gctgccgccc gcttggaaca agccgaattg acccgcgaac gcctgcgcct
    22141 cgccgcgggg gaagccgccc gcgaagcact ctggttgctg gcagccgacg atgaccgcca
    22201 agtggaccac ctgggcgaag attgggccaa accccatgaa aaccagcgtg acgatggcgt
    22261 ctatacgtgg tcactgatcg aagacgccgg gcgatttttc aacctgaaca atctggcggt
    22321 cgccacccaa gggccacgcc cagccaacga aatcctgctg gatttgctaa ctttttgcgg
    22381 cgatttttcg ccctcggtcc gcgtggccgc gctgacggat tttgtggatg gcgatgaaga
    22441 cggtccctat gaggcggagt tttaccgccg cccaacggat ccctaccgtc cgcccaaccg
    22501 cgagttgtgg gcgccggcgg aagtgctccg ggttcatgac ttcactgcgg aattgttccg
    22561 gccgcgcccc cgcgggccgg ccgacgatct gttcggcggt gatttctcca gcgcggcggt
    22621 gattgtgccg cagcggttga acaagccggt gcccatcaac gtcaacaccg ccagccggga
    22681 tgttttgatg ggcctgacca gtctgcagca cgatgccgtg gtgcggaagg tcatggccat
    22741 tcgccaaacg caaccgtttg agtcgctggc gatgattttt gcggcctatc cggaatttgc
    22801 ggcagcgttg gcggatagcg tgtccaccgc cagtacgatt ttccgggtgc gtgcgcgggc
    22861 cgcggcggac tcgcaacgct ggtcggtcat ggcgtgggtg gagcggagtc cggataccgg
    22921 cgccatccgc attttacaat gggtggaggc aggtggctga caccatggaa caccccttcc
    22981 agatggccta tggcctggac atggccgacg ggcagtggat tctggcccag tgcgcgcgcc
    23041 gcggcgtggc gcaagtgcgg ctggccgcgc cggcggagtc cgaggaaatg cgccgcgcgt
    23101 tgcaggcggt tcaagccgat gtggaacaag gccgcgccgc gttggcggtc agcgccccgg
    23161 ccgcgcagac ggtcgtgcgg cgattgcgcg cgccattccc ttcgctggcc aaggccgcgc
    23221 ggatttggcc ctcgctactg gatgtggatt tgccgtttcc cgtggaaagc gcggtcaccg
    23281 gctttgtgca gccgcgcgtg gtgcaggggc aggccaccac gcttgccgcc gccatccggc
    23341 ggagcgattg gtcggcattt gaagtacaat gcaacgccgc cggcgtgcag cccacccacg
    23401 ccgatgtcga agcgctcgcg ctctgggaac agcagagcgt ggatgcgcca tccgcccgcg
    23461 cggatgcctc gcggctgctc atctggcttg gcgcggatca cgtgaccatc gtccgggggc
    23521 ggggtggcga tttgctcggc gcgcacgtgt tgcgcgcaac tccccagccg agcgggagcg
    23581 gggattttgt gacattgtgg gccgcgcggt tgcagcccat tgttgccgca caagcaactg
    23641 aaaccgacgg cgcgcccatg gatgtctggt gggccgggcc tagagcagaa gacgccgaat
    23701 gggtggcacg cctgcgcgcg gccatgccgg ctgttggcca gatgcggcat gaaacccatt
    23761 cgctggcggc ctcgctgctg gcgcgggcct tggcgcgccg cgcagcgggt aacggcggca
    23821 tcaatttcaa atcgggcgcc gatacgcatc cggccttgca ccgcgcggcg cagcgccgcc
    23881 ggcagcgagc ttatcttggc gtggtgattg ccgcgctggt ggtgctggcc ttgaacttcg
    23941 gcgagcggca tctgcgccag cggcagttgt cggccgcgca gcagaacttg agccatttgg
    24001 cggaatcaat cacgggtgcg ccggtggtgc gcgggcagga gttgctgttt gtggagcgcg
    24061 cgctggccca gcgcgatgaa gacacccgtg ccttccgcga agccatggaa gaagatggac
    24121 tggaagaacg cgtggcgcgc gtggtgcagg cggccgatga actgcacgtg gagatcgtcc
    24181 gcctgaacat ttcgccggtg gcgctgacgt tgcaaggcag cgcgcccggc attcaggttt
    24241 tggagacctt cgcggaaaaa ttgcgcggct acggctggca ggtgcaggcg gatacctccg
    24301 ggctggcact gggtggccgc ccccaatttg tgttgaaagg gatgcatgac caagtccgct
    24361 gaacgctatt ggcaggccgg cgctttgctg ctcggcgtgt tggccgtggc cttcacgctg
    24421 gatacatggc tctcggcggc acggcaccgc gacatcctgg ctgccaagga agaggcgggg
    24481 caaaaggtag ccgcgtatgc cgggcgctgg gcgcgcgagg atgcgtaccg cgactcgttg
    24541 gaacgccagc aggcatggca tcctgtggaa ttggatgacc tggccacgcg cctgcttggt
    24601 ttgcatacgg tgcaaatcac gccgcgtccg gccaccgcgg tggctgccgg ctggcaacgg
    24661 cgcgaggtgg cgattcagat gaataatgtc acctatgccg aggccgcgtt gtttttggct
    24721 gatgccgccg ccgggctgcc gccatggcgg ttgcaggatt tgacgctggc gccagcggct
    24781 gaagccggca agggcaactt ggccgccgtg ctggttgcgc tggaaaagaa acagccatga
    24841 gtctatggtg cgcgtgatga aattgttgcc caccatttgg aatcgccgcg aattgctgtg
    24901 gaatctggcc gtgcgcgagc tcaaacagcg ctatcgcggc tctgtgctgg gttttctctg
    24961 gacgctgctc acgccgctgt tcatggccgt gatctacctg tttttcctgc gcctgctggc
    25021 cgggcgcggg gtgccgatgg aagaaatcat catcggtgta tttgcttggc agttcactgt
    25081 gcagtgcacc caagccggta cgacctgtgt gctgggcagc gcaaatctcg tcaaaaaagt
    25141 ggctttcccg ctggagattt tgccgttgag ccaggtgctg gccaacacca tcagctatct
    25201 gctgtcgcta gtggtgcagt ttccgctgtt gatgttttta ctgtcgcggt cgaatcagca
    25261 tctggccatg acatggccga tctttttcct gtgggtggcc tggcaaacct tgtttaatct
    25321 ggggctgact tcgtttgtcg gcgcgctcaa tgtgttttat cgggacacac agcatctggt
    25381 aggccttgcg ctgagcgcct ggttcttttt gagcccggtg atgtacaaca tggaattcgc
    25441 cctcaatttg gccgcgcagt ggccgctggc cgcggacttg ctctatgcga accccatgac
    25501 ggtgattctc aacggctacc gcgcggcgtt gctgccggag agcgcagccg cgtacccacc
    25561 cgcggcgtgg atcgggctgc tgctggtgcc agtcatgttc attctggggg tggtggtgta
    25621 ccgccgccgc caacatctat ttgcggatat gctctaaaat gaacgccatc gaagcagtcg
    25681 gtttgggcaa gcgctactat cgccgcggcg gacagcgcgc gaccacgttg ctggaaacgc
    25741 tcaccggctt cggccgagcc cgtcgcgaag agttctggtg cctgcgcgag gccaacttcg
    25801 ccattccggc tgggcgcacg gtgggagtga tcggccccaa cggctccggc aaaagctcgc
    25861 tgctgggctt ggccgccggt accatcacgc ccacggaagg ccatgttcgt acgcagggcc
    25921 ggatttcctc gctgttggaa ctgggcgcgg gtttccatcc ggaattgagc gggcgcgaga
    25981 atgtgtatct caacgccgcg ctgctgggca tcccgcgcga agacgtcaaa aagaagttcg
    26041 accaaattgt ggattttgcc gacttgcgcg agttcattga catgccggtc aagcactact
    26101 ccagcggcat gtatgtgcgg ctggggttct ccatcgccgt ggaggtggat cccgacattt
    26161 tgttgattga cgaagtgctg gcggtgggcg acatcgcctt ccaactgaaa tgcctcgacc
    26221 gcatccgtca gttccaacgg cgaggcaaaa ccattttatt tgtgagccac gctctgcaga
    26281 cggtggaaga gttctgccac gaggcctttt tgattcatgg cggccgtctg gtggaacgcg
    26341 gcgagccgag cgaagtcatc ctgaaataca tccgccgcta catgggcgag gggggcgggc
    26401 tgttcacgca ggaattcggg acgcgggcag tcgagtttgg caacgtacgc ctgctgaatg
    26461 ccgctggcga ggaaacaggc atctttgaaa ctggcggcgc gctgacaatc gaaatcccgt
    26521 acgtggccca ccggcgcata gaaaaaccgg ttttcggttt ctcgctcaag acgggcaacg
    26581 gaatgtatat ttacggcagc aatacccaaa tccagcaagt ggcggttccg ccgctcgaag
    26641 gcgcgggcgt agtgcggctg gattttgcac cgctgcggct gatgcatggc aactactttt
    26701 tgtcgctttc gatccactcg tgggatcatg ccgagcagtt ccaccggcgg gaagattggt
    26761 ggccattcgg cgtacgcaat acgggggaag acctggggct gttcaaactg gacgcccgct
    26821 ggcattgccc ctgaacccgg cgtgttttcg cgcttcgcgg ccaccaccat tcctagcttg
    26881 cgtcccgcgt gcggaattcc tacaacattg cgccggttgc cgggtgcgcg gaaggcgcaa
    26941 ccccgcggca tagtggcaag gagacaatcg tgaagaaaga aactcggcgg tggatggtct
    27001 ggtggctggt ggtgggcatg gctggcatgg tgttggcatt gaccggttgc gaaagcgatc
    27061 acaacgacga aacaatggaa gaaatccagg ccgaagccac caactcggtg aataatgccg
    27121 attcccccgg cgacgaagat cacggcctca acgagttttt ctatgatggc ttgcacccga
    27181 cccgtgccgg caacaaggtc attgcccgcg tgtttgccaa gaagatcaag ccgctcgatg
    27241 atgccggcgg caacaacgtc aacgtcatcg tgtgcctggg tgacagcatt accgacactg
    27301 gctatccttc cgacttggcc aaactgaccg gcatgacggc ctatgaagag ggcattcgcg
    27361 gcgaacgcag ctccggcggc gcgtcccgca ttgacggcgt gctgtcgggc tacaagccgg
    27421 catatatctg cattctttat ggcgcaaatg atgtgatcgg ccatctgcct gcatccactg
    27481 tgaccggcaa tctcgaagcg atggtgattg ccgcgcgggc caacggcacc attccaatac
    27541 ttgcgacgtt gacgccgatg ttcggcccgc gcaagcagta tgccgccgat gcccgggcca
    27601 ccagcgcggc catccgcgat ttggcggggc ggttgggcgt accgttggcg gacctcgaaa
    27661 aagcatttta gatcgggaaa gggcggacat gcagaaacgg cggcaatggg cgtggtggtt
    27721 gacgattggc ttgtgtggaa cccttctact ggcaaccggg tgcgaaagcg atgacagcca
    27781 cgagaccccc gcgcagattc aggaggaagc gcaaaacact ccgccggtca ccaacccggg
    27841 cggcaacggg ccatctgaat ctacgaccgg cacgccgtcc acttcgccgc cgagcgcggc
    27901 caccgtgcgc aagtatttcc cgggcggcct gcatcccaat acggaaggca gccggatcat
    27961 cgccaaaacc tttgccggag cgatcacacc ggtcagtgat gctggcggca accgcccgaa
    28021 tgtgattgtg tgcctgggcg acagcatcac cgatagtggt tatcccacat atctggccgg
    28081 gatgaccggc atgaccgcca ttgaggaagg catccgtggc gagcgcagcg ccggcggcgt
    28141 gtcgcgcgtg ggcggcgtgt tgagcaaata caaacccgcc tacctgtgca tcctgtatgg
    28201 cgccaacgat gtccgcaagg gactgccggc ctccaccgtg acgggcaatc tggagaagat
    28261 ggtgaatgcc gcgcgcgcca acggaaccat cccgattttg gcaacgctga ccccgatggg
    28321 cggcacctat tccgagttca ttcccgaggt gcaaaaaacc agcgcggcca tccgcgattt
    28381 ggcggcacgg ctgggcgtgc gcctggccga tttggagagg gcgttttagg cgggcagtta
    28441 aacgccggat agcgcgattg acgtggcgcc gggaaagggg ttgccacgcc ctgtctaaaa
    28501 acctacaact ttttttcaag catggatccc gttgccccag attttgccgc ttctgcctgc
    28561 cgtggcgagt ggcagccgca tccgccggcg ggaatgctgt cggcgattgg tgtggatagc
    28621 cggactgtgg cgcgcggcag cctgtttttc gcgctgcccg gcgcacgggc ggatggccac
    28681 gattttttgg ccgatgtggt ggctgcggga gctcatgccg tattgcgcgc ggattacccc
    28741 gtggataagt tgccgactgg ggggcatttc ctgcgcgtgg acaatgtgtt ggatgcgctg
    28801 ggccggtttg ccgcggcata tcgcgccacg ctgcccactc gcatggtggg ggtgacgggc
    28861 agcgttggca aaaccagcac caaggaaatc attgcggact tgctatgcac cttgggcaaa
    28921 accggtcgca cggtcggcaa ctataacaac gaaatcggct tgccgttgag cgtggctgcg
    28981 ttggatcgcg agtgccgttt cggggtcatc gaggccggta tcagtcaccc cggcgagctg
    29041 gctcatctgc gcgatatttt gcggccggac atggcggtga tcacccgcat cggcccggtg
    29101 cacgcggaat tttttgattc cgtgcgtgac attgccgaag aaaaggccac gctgctgacg
    29161 gcattgccgg cggatggctt tgccgtgctg gatcgcgatg atgaattttt cccgatgctg
    29221 cgtgcgcgct gtagcgcgcc ggtcatcact tgttcgctgc gccggcgtga ggtggattac
    29281 gccggcgacc tgcaagagga tgaccaacta tgggtgtgcg aggccgccac cggcgaaacc
    29341 gcgatgctgc cggtgccccc gccggcgtcc tacatggccg aaaatgtatt gcgtgccgtg
    29401 gccgtggccc ggcggtgcgg tgcaacgtgg ccggccatcg cggccgcact ggcgcaagtg
    29461 aaaccagtgg gaatgcgctg ggccgtcagt gagtggcagg acatcaccat catcaacgat
    29521 gcctataacg ccaatcccat cagtgtgcgc gcggcggtgc aggcatttgc ggcgtggccc
    29581 gtgcgcggcc aacggttttt ggcgataggg ccgatgctgg aactgggccg gttggaagcc
    29641 accgaacacg aggcgctggg gcggcatttg gccactggcc agtgggccgg cattgtgctg
    29701 gtgccatgga aaattcacgt cggcgcaccg gatccggcca cacaggcgct ggcggccggc
    29761 ttgcaggcgg gcgggtggcc cgccgaaaaa atgtgtctgg ccccggatgc cgcggcggcg
    29821 gcaacgtggc tgcaagggcg ttgccgtgca ggcgacgcgc tgctcttgaa ggcatcgcgc
    29881 ggtgtgcgga tcgaaaccgt tttgactcat tggcaaaagg aaagctgaac ccatgttgta
    29941 ttatttgtcg ctgctgtccg agcattactc cccgctgaat ttgttccgct atgtcacgtt
    30001 ccgcgccttc atgggggcgg ggacgtcttt tctgctgtca ctgctgttgg ggcggtggat
    30061 gatcggtcgg ttgcgtgcgt ataaaatcgg ccagatggtg cgtacggatg ccgaactggc
    30121 ggcgattaac cacctcggca aaaaaggcac accgaccatg ggcggcctgc tgattatttt
    30181 cactacggtg ctggcggcgc tgttgtggtg ccactcgtcc aactcgcagg tgtggctggt
    30241 gctggccacc atgctgttca tgggtggtgt gggatttgtg gatgactggc tcaaactgaa
    30301 aaaacgcggc tccgaaggcc tgcgcgagtg gcagaagctc gccttgcagg gcgtgtgggt
    30361 ggtgattgcc tttgtggtga tcgaggcggt gccggccacc catttttatg cgcggcagtt
    30421 gatggtgcct tttctgaaga cgccgttgtt cacggatatc accgtgcccg gcgcgctgct
    30481 gttcatgttc ctggtgctgg tgggcacgac caatgccgtc aacctgaccg acggactgga
    30541 cggcttggcg gtggggtgcg tgaactcggt gtcggcggct tacttggcgc tggcctatgt
    30601 ggcgggcaat atcgtcatgg ccacttatct gcaagtgccc gcgattgccg gcgcgcatga
    30661 attggcggta ttcagcggct gcctgttggg cgcgggcctc ggctttctct ggtggaatgc
    30721 ctatccggcc aaagtgttca tgggcgacac cggcagcctg gcgctggggg gaaccatcgc
    30781 catgattgcc atcctgatca aacaggaact gacgttgttg atcgtgggcc tcgtgtttgt
    30841 ggcggaggcc ggcagcgtaa tgatccagcg cgcagcatgc cggctctacc ggtggcgcac
    30901 gggcacggga ttggcgcagg accggcgccc cttccgcatg acgccgctgc accaccattt
    30961 tgaaatcatc tcgaaggaac gcgccaaagc cgaaggccgt tcaccggatg ccgcagaaaa
    31021 ttcggtggtc atccgcttct ggattgtttc gattgtctgc gccctgctgg gcttggcgac
    31081 gctgaaaata cgctgaccgc catgcaacgc tattcccacg cgatggtgtg cgggctgggg
    31141 gccagcgggg tggccgccgc gcagttgctg cgccgcgaag gcaccaccgt catcgccgtg
    31201 gacgagtttg acaccgccgc cacgcaacac gcggcggaaa ccctgcgcgc ggccggttgc
    31261 cgcgtggagt tgggttgcgc cgcgccacca gaagtaaccg ttgatgtggc catcgtgagc
    31321 cccgggctgg ccctgcattc gccgctgttg gtggcgctgc aaaagcgcgg tatcccgcta
    31381 ttgtccgaaa tggagctggg gtggagccgt catgccggtc gcacggtggc cgtcactggc
    31441 agcaacggca aaagcagcgt ggtcaaagca ctggcggatt gtttcgccgc cgcgggccac
    31501 aaggctgtgc catgcggtaa ctacggcttg ccggtttgcg aggcggtcat gacggacacc
    31561 gagtggctgg tgattgaagc cagctcgttt caattggaaa cgtgcgccgg ttttcgcccg
    31621 gatatcggca tcgtgctgaa catttttccg aatcatttgg accggcatgg cacgttggaa
    31681 acctatgcaa agctcaaggc ccggctgttg tcgcgccaac gtgcgggtga tgccgcgtgg
    31741 gttccgccgg agtgggccgc gtcattgcgg gcgtggtcgc aaggacaagg gacatggcac
    31801 actttcggca ccacgaccca agaggaagtc cattacgccg cgcatcagtt ggtggcccgc
    31861 gacagtcgtg cgttcaacat cgctggttct tattttgaca atgaagtgct gggcgccaat
    31921 gctgccgcgc tgctgggcgc ggcacaggct gccggtctgc cgccggcgct ggtagcgcag
    31981 gccttggtga actttcaacc tctgccacac cgcgccgaat tggtgggcga gatcaatggc
    32041 gtgcgctacg tcaatgattc caaggccacc aacctttccg ccatgctggc ggccatccgg
    32101 atgcagcggg gagcggtcat cttgattgcc ggcggccgcc ccaaggaaaa agattttgca
    32161 gcggcggtgc ccctgttgcg cgaacgagtc cgccgcgtgt tgctcattgg cgaggctgct
    32221 gctgccatgc aggcggcctg gggcgcgcat gtgccctgca cggattgcca cactctcgcg
    32281 caggcggtgg ccgcggcccg gcaaaccgcc cgccccggcg agacggtttt gctggcgccg
    32341 gcctgcacca gctatgacca atttcgcgcc tatccggagc gcggcgaggc ctttcgcaaa
    32401 tatgtgacgg aaaaggattg aatttcctgc gggggaatgc gaataatccc cgcgaatcgg
    32461 tgaaccattt tgacgaagaa acaagaagga gaggaatcat gagggtatcg gtagtggcca
    32521 gtttgatttt ggctgtgcat gttgcggtga ttggctccgt ggtaatgacg caaggttgcg
    32581 ccagcacccg gcagggggct tccacgacgc ccgcgcaagt tgattctccg ccggtgccgc
    32641 cgttgccgcc cgtggcggtg gatatgccga cggtggcgca gccggtggcg ttcccggtga
    32701 ttcagccgcc cgcgcccccc gcgccgctga agacttccgt ggccgcggaa aatgtgtacg
    32761 tggtgaagcc cggtgacatt ctctccaaaa ttgccgcggc gcatggcgtc aagacggctg
    32821 agttgaagga actcaaccag atcgctgatg ccaacaagat tcgcgttggc cagaagctga
    32881 ttctgccgga ttacgccaag ccttcgcagt cgcagcccgc ggccaaggaa agcaaggccg
    32941 ccgccgcgcc tgtggcggct ggtggcgaca cctatgtggt caagtccggc gatgcgctgt
    33001 ccaagattgc cgcggcgcac ggtgtgaagg tcaaagacct gatggccgcc aacaacatca
    33061 ccgacgccaa caagattcgt gttggccaaa agttgacgat ccccggcgcc aaggccgcgg
    33121 aaaagacgga gaaggccgac aagcctgaaa agactgacaa gcccgcgaaa cccaaaaagg
    33181 ctgaatcgga agcggaagtc gcgccgatcg agccggttgc cgcgtcgcct gccgaaccag
    33241 ctaatgttcc ggatcaggat aagagcttgg aatatcctgc ggcccccggc gacacgattg
    33301 aaagcattgc ccgcttgttc tccgtgccgg tttcggcgat caccaaggcg aacaatttgc
    33361 cggtggatgc caagctggaa cccggccaga aattgctggt tccacaaacc agcccctaaa
    33421 acggcaattc cgcggatttg ccaacaacgc ccggtctgga cagaccgggc gttttgcgca
    33481 atcaatagcc gggtatgaca tcgcgtgtgc ttttgatttt ggtggtgggg gcgctggtcc
    33541 tgtttggcct ggtgatgctc ttcagcacca gttggccgca aggcgaaaaa atcagcaaca
    33601 accagtatta ctttgtgcag cggcaggcca tttggttggc gctgggcttg gtggcggctg
    33661 ttgtcggctc ccgtgtggat tatcatgtct ggcaaaaaat cgcctggccc atcatgggcg
    33721 gggtgctggt gctgctggcg ctggtctatg tgccgggcat cggattgagc atcaaaggca
    33781 gccgccgctg gctgcagttg ccgatgaagt tgacgtttca accgtcggag ttggccaagt
    33841 ttgctgcgat caatgcgctg gcctgctggt atggagcgcg ccgccgcggc gggggaacct
    33901 ttacccgggt ggtggtgttt cccgcgttga ttctgggctc gtttttgctg ctgattttgc
    33961 cggagattga tgtcggcgcc acggtattga tcggcgccac cggcggcgtg atcatgctgg
    34021 tggcgggcgc gccgttctgg ctgctggctt tcggcgtggc gctggcggcg ggcggcttgg
    34081 gggttctgat caaatatgat cccgtgcgca tgagccgcgt gctggcattt ttgaatccgg
    34141 aagaatatgc ccagaccgaa gccttccaac tgttgaacgc gctctacgct ttcgtggccg
    34201 gcggtcccac cggggtgggg ctgggccaga gcttgcaaaa acagcactac ctgcccgaag
    34261 cgcacactga cttcattttt gcgatcatcg gcgaagaact gggcgtggtg gcatccctca
    34321 gcgtggtagt gctgtttgtc attttgctgt tgtgcggtat tcgcatcagc gggcgcgcgc
    34381 cagatgtcag cggccggcta ttggctttcg gcatcacctt tatgatcacg ttgcaagcag
    34441 ccatcaacat tggcgtagtg accggctgcc tgccgacgaa gggtctgcca ctgccgtttg
    34501 tgagctttgg cggcaccagc ctgatggtca cgctcttcat gataggcgtg ctgctcaaca
    34561 tcagccgcca aggcatcacc cgcacccgca cagcgtaggg gggcagcgta acggcgcatc
    34621 cacccgcccg gtggattatc agggcgagat attgccaagg gggggagcga ctgttataca
    34681 taaacaaata tggctggcaa aaaaggcaag ttgacgaaca cccgtcgcga ggtcacgggt
    34741 gttgtgctgc agggtctgtg gattctgctt ttactggcct tggcatcgta tgatgcgcgg
    34801 gatacatccg attgggccag cgcggggcac gaaacgctga actacatcgg gccggtgggg
    34861 gcgtgggcag cgtgggtggt ttttcggttg ttcgggctgg ccggctttgt cctgccggca
    34921 ttgctggccg tgtgggggct gttgatgatt tttcaatcgg gcgaacgagt ctggccgcgc
    34981 gcgctttgga tgctgggcat cctgtgcggc gtatgcgtgt tgatggatat ccaactgccg
    35041 ttctggaacc gaatttcgcc ggaacggttc aatctgaacc agatgcccgg cggactggcc
    35101 gggttgttcc tcgggcggat gttcctgacc caatatctgg gcgtggcggg cagtgcgatc
    35161 attagcttgg tggtgatgat cagcgggctt ttctttgtgt cgcaaactca tccgattgaa
    35221 ctctggcggg cgattgccga tgcctggcac aactggcgtg aacgacgcga agagcgcgcc
    35281 gcacagaacc gcaccgcgca agagcaggcc gagcacgaag ccctgaaact cgaacgcgaa
    35341 cgcttgaaac tcgagcgtga actggagcgc gagcggcgca aacaggagat ggaagaccgc
    35401 cgtcgccagc gggagcgcga gcaggaagaa cgcgaagccg cgcgtcagcg cgaagccgcc
    35461 gagcgcgcgc agcgtgagcg cgaagcgcag gaagcgcgcg aacgcgaaga agccgcccgc
    35521 aaggccgaat ttcagaaacg tctggccgag cagcaaaaag cggaggccga acgccgcgcc
    35581 gcagaagaac gcgccgccaa gcaggcgcgg gttgaagcgg aaaaggcggc taaacgcgcc
    35641 gcggaggaag cctcactgcc gccgccgctc tggacactgc cccccatgtc gttgctggcg
    35701 ccggttccgg aaggcgcggg ggcccgtgcg ggcgcggacg agttggaccg caccatccgc
    35761 gtcatccgcg gcacgctggc ggagttcggc attgatgtcg aagtcacgca cgtggagcag
    35821 ggcccggtgg tcacgcgcta cgaattgctg ccggcggcca acgtcaagct ggagcgcatt
    35881 agcgggctgg ccaacaatat tacactggcg ctgaaggccg agagcatccg cgtgcaggcg
    35941 cccatccccg gcaagggagt ggtgggcatc gaagtaccca accggcacgc cgcgcccgtg
    36001 gtgttgcgcc agttgctgga aagcgccgcc tggaccacct ccaaggccgc actgccgttg
    36061 gcgctggggc aggatgtcag cgggcatgtc ctgatggcgg accttgcctc gctgccgcat
    36121 ttgctgattg ccggcgccac cggccaaggc aagacggttt gcatgaattc catcctcgcc
    36181 ggcttgctca tgacccgctc gccggaggaa ttgcgcctga ttctgattga tcccaaaatc
    36241 gtcgagtttt ccggttataa caacctgccg cacttgctgg tgcccgtcat caccgatgcc
    36301 aaaaaagtgg tggggggcct gcgcgtggcc attcaagaaa tggagcggcg cttcaagatg
    36361 ttccaccgcg ccaaggtgcg cgacatccgc tcgttcaacc agcgtcccaa agcagcgcag
    36421 gcggccttgt tcgatgaaac ggaattacaa aacgcgcagg ccaccgaaga tggaatgccg
    36481 ctgcccccgc cagaaaccat cgaggaaacg gaggagccgt tgccggaacg cctgccgtat
    36541 tgggtgatca tcattgacga gctggccgat ctgatgctga ccgcgcagca ggatatcgag
    36601 ccccgcatcc aaaagctgac gcagttggcg cgcgccaccg gcattcacat gattatcgcc
    36661 acgcagcggc ccacggtgga catcatcact ggcaccatca aatcgaatat ccccggacgc
    36721 gtggcattca aggtggccca gaagaacgat agccgcgtgg tgctcgacca ggaaggcgcc
    36781 gacaaattgg tcggccgcgg cgatatgctc gtgctgaccg gcgccaacaa gatcgtgcgt
    36841 gcgcagggcg cgtggaccaa ggatgaggaa atcctggcca tcgctgagca ctggaagcaa
    36901 cagggcgcgc cgcgcttcga tcagcgcctg atgggcaagg agggtaagga gggcggcacc
    36961 gatttgccgg atatgccgga agaggacgaa gaacttctcc agcaggccat ggaagtgatt
    37021 cgcctgacgc ggcgggccag cacatcgtcc atccagcgtc gcctgcgcat cggctacacc
    37081 cgcgcggcgc gactgatgga tattctcgaa gaaaagggct tggtcgggcc gtcgcgcggc
    37141 gcggaagcgc gcgaaatctt gtttgatatt gacgggttgg ggcagggggc caatggcgcg
    37201 gatggcggcg aggatgagcc gctggcatag gatggcaaca tgagcgggat cggcagccaa
    37261 ttaagggaag cacgggaaca ccgcggggtg acggttgggg aagcggcatc ggccacgaaa
    37321 cttaaagtac tcgtggtgga cgccatggaa cgcgacgatt accgccggtt gattgccccc
    37381 acctacgcca agggattcta caaactttat tgcgattatc tgggcctgga tccggagccg
    37441 ttcattcagg cctatctgca tttttcgggc gagtcggacg acaaaaccga actgatccgc
    37501 gatccgaaaa agaagcccgg cttgttctcc ggcctgcaaa agaaaatgaa ggatttgcag
    37561 gaccgcaagg aaatgcagcg caaggccaag gaaatcgccg aggcacagga aaaagcgcgg
    37621 caaatgggcc gccagcacgt cgcggcagaa ttggccggtg cggaaccgcc ggcgcaacag
    37681 cccgcattgg ggcagcagga attgccgctg ttggaccatc cgcagccaac tcccgagcca
    37741 ccgccggctg aagaagagcc agagttgatt ccggagccgg agccggaggt ggaagaggcg
    37801 cctgcgccgg aagagcctgt cctgcaagtg gtggctcagc cggagccgcc tccgcctccg
    37861 ccgccacctg cgcccgcgcc ggaacggcgc acccgtgccg ttggcaagaa aaaggcgccg
    37921 gtgtcgcccc gcgaggaagc cgaagaacgc gcgcggattg tggaggaacg gcgccgccaa
    37981 ttgcaggagg cgctgcgtca atccgaagac gaattgcgcc gtgcccacga ggaagcggaa
    38041 cgccgcgcgc gcgaggaagt cgaacgcaag gcacaggaag cggaacaaaa ccgccgccaa
    38101 ctggaaaaag agctgaagaa acgcgaagcc gccgagcgcc aagcccgcga agaggccgag
    38161 cagcgtttgc gcgaagaagc cgcgcggcgc gccgaggaag aagccaaact gctcgcgcgt
    38221 gaagaagcgg aacgccaggc ccggctcgag gccgaacaac gaatccgcga gattgaagag
    38281 cagcgccggc agcttgaaga cgagctgcaa cgccgcgaag aggccgcgcg tttggagcgc
    38341 gaggccatcg aacggttgtg gcaggaagaa gaaaacaagc ggctgcaggc cgaactggaa
    38401 caacaggccc gagaggccgc ggaacggcag gcgcgcgaag ccgccgagcg caaggtggcc
    38461 gaaaccgagg ccagccgccg ccagttggaa gaagaacttc gccagtttga agaaaatgcc
    38521 cggcgcgccc gtgaagccgc cgagcagcgc gcccgcgaag aggccgagca gcgcgctcgc
    38581 gaggaagcgg aacgcaaggc ccgcgaagag gccgaacgca aggcccgcga aaaagccgaa
    38641 cggcaggtgg aagccaccga agccaagcgg cggcaactgg aagaagagtt gcagcggcgc
    38701 gaggtggaag cccgcaaggc ccgcgaagaa gccgagcgca aggcgcagga ggccgaagcc
    38761 caattcgccc gcgaaaaggc cgagcaggaa gcgcgcgaag aagccgagcg cgaggcccgg
    38821 ctggcggttg aacagctcgt ccgggaaacc gaagaacgcc gtcgccaact ggaagcagaa
    38881 ctcaagcggc gcgaggaaga aacccgcttg gcgcgcattg cggccgagga acaagcgcaa
    38941 ctggaggcgc agcgccgcgc cgaggtggaa gccgagttgt atgcccgtag cgaggcggag
    39001 cgcgcggcgc gcgaggcggc cgaagaaaaa gtggcagcca ccgaagaaca gcgccgccaa
    39061 ttggaagaag agttgcaccg ccgcgcagaa gaagcggcct tggcccaggc ggcggcggaa
    39121 cagcgcgcgc gggaggaagc tgagcatcgt gcgcaggtcg aggctgaact acaggctcgt
    39181 ggcgaggcgg agcgcgaggc tcgcgaactc gccgaagcca aagtagccgc taccgaggaa
    39241 cagcggcgac agcttgaaga agaattgcgc cggcgcgagg aagaagcccg gctggcggca
    39301 gaagaggcgg aacgtcgcgc gcaggaagaa gcggagcacc gcgcgcaagt cgaagccgag
    39361 ttgcaggccc gcggcgaagc cgaacgggta gcccgcgaac tagccgaaga aaaggtacgc
    39421 gaaaccgaag agcggcggcg ggcgctggag gccgagttgc agcggcacga agaagaagcc
    39481 cggttggcgg ccgaggaggc ggaacgccgg gcgcaggaag aggccgaaca gcgcgcgcag
    39541 gtagaagccg aattgctggc ccgcgaagag gccgaacggc aggcgcgcga ggccgccgaa
    39601 caacaggcgc aggaacttga ggcgcgtcgc gagcagttgg aagcggaact gcaccggcaa
    39661 gaagaaatgg cccgccagga gcgggaaaag ctggaatcgc tactgcaaga ggcggcggaa
    39721 cggcgcgcat tgatggaggc cgaacaccaa cagcgcgagg aggccgaacg gttggcccgc
    39781 gaggaagccg aagcccagca gcgcaagctg gaagaggcct tccaacagcg cgaagtggaa
    39841 gtgcgcatcg cgcgcgagga ggcagtgcgc ctgcacgagg agtccaaccg ccaaacccgc
    39901 gaagcagccg agcacaaggt gcgcacggcc cgcgaggcgg ctgaacagaa agtccgcgag
    39961 attgaagaac gccggcttgc gttggaacag gaactccagc agcgcgaaga agaagccgct
    40021 gcggcccgcg aagaggcgga acgccgcatg caggaggaaa tcgagcgccg cgcgcaggtc
    40081 gaggccgagt ggcgcgaacg cgaggaagcc gagcgcaacg cccgcgaaga agtggaattg
    40141 caggttcagg ctgccggcac ccgcttggcg gaactggaag ccgagttgcg tcagcgggaa
    40201 ctggaagcgg tcgccgcccg cgaggaagcc gagcgcaatg cccgcgaaga ggccgagcgc
    40261 cgcgcgcaag tcgaagccga atggcaggcc cgcgaagaag ccgaacgcgc cgcgcgtgaa
    40321 gaaatcgagc aaaaagccca gcaaaccgcc atcaaactgc gttcgctgga ggatgaactg
    40381 gtcgcgcgcg aggaagaggc tcagcaggcc cgggaggaat tggagcggcg aatctgggaa
    40441 gaaagcgacc accgcgcccg agttgagcag gaattgctgg agcgggagga agccgcgcgc
    40501 gtcgcgcagg agtcgtatga ggccgcagcg gcacgggcgg cggacaaaat ccgtgaaatg
    40561 gaagctgacc ttgcacgcgg cgaggaagaa gcccgacagg cgcaacttga attggaacgg
    40621 cgcattcagg aagaaagcga ccaccgcgcg cgggtggagg aagaattgca ggcgcgggaa
    40681 gcggcggcgc gggcggcgca ggaggcctat gaggccgatg cggcccgggc ggctgacaag
    40741 attcgcgaca tggaagccga gttggagcgc cgcgaggaag cggcccgcct ggcccgtgaa
    40801 gaagccgccg aacgcgcccg tgaagaggcc gcgcagcgcg cgcgggttga agccgaactg
    40861 caagcgcagg gcgaggccga gcgtcaggcc cgcgaagcgg ctgagctcaa agtccgtgtt
    40921 tccgaggaac gcctggccga gttggaacag gaattgacgc gtcggacgga agaagtccgt
    40981 caggcgttgg ccaacgccga acagcaggcc cgcgaagaag cggcgcggcg cgcgcaaatg
    41041 gagcaggatc acaagcagcg cgccgccgca gagcaggccg cgcgggcggc tgcggaaaaa
    41101 gaagtcgaaa aaacggaaga acgacggcgc aagctggaag aacagttacg ggccaaggaa
    41161 cgcgaggtgg tcgtggcgcg cgaagcggcc gaagcccgcg ccgccgaaga agccgctttc
    41221 cgcgcgcagc aggaggccga acaagcggcc cgggccgcgg ccgagcagga agcacgggcc
    41281 gccgcccaac agaaaattgc agaggccgag caacggattg cccaactggc ggcagaactc
    41341 caaacccggg aagccgaagt ccgccaggct cgcgaagagg tcgttttgcg ggttaacgcc
    41401 gaggccgaag cacgtacgcg cgaagagtcc gaacggcagg cccgcgagca agcggaaaag
    41461 gaagagcgcg cggctgccga ggcgcggttc cgcgccgagg aacagcgccg gctgcaactg
    41521 gaagaggaac tgctgcgtcg tgagcaggag gcccaacagg tccgcgccga atccgcgcag
    41581 aaaattgcgg ccgaggctcg cgcccgcgaa gaggccgaaa aggaattaac cgcccgcatg
    41641 gctgctgagg ccaaggcgcg cgaggaagcc gaacgcaagg tacaaaaggc cgaagaattg
    41701 cgccgtcagt ttgaagaagc cttgcgggcc aaggaacgcg aagccgccgc ggcccgcgag
    41761 gaaattgaac gtattctggc ggaagccgaa gagcgccgcc agcgtgaggc gtcggaatgg
    41821 caatccagcg aagcgtccat ccgccaacag cacgaacagg aacttgcgcg gttgcaaaag
    41881 gaagccgagg cgcgcgtcgc ggcggagttt gcgcgccgcg aagaagagcg ggtggccttg
    41941 gccaaacagg aagaagcgct gcgcttgcag cgcgagcagg ccagccgcgc ccaggaggaa
    42001 ttccaggcgc agttgcagcg gcagcaggaa ttggaagaag ccaaccgcgc catggcggag
    42061 gcgttggcca cccaggaaca attgcggcag gaactggaga aaaaactggt cgaggccgaa
    42121 cggcgtcgcc aagaactcat tgcgcgggat cggacagctc gcgcgcagcg tgccgcccaa
    42181 gctcaaacga ccacgccggt cattaaaacg accccgccac ccgcgccggc gaccgttgcc
    42241 caagtttcgc cggccgcgcc agtgtcaaag aagcagcgca ttccgctgcg cgcgcggtgg
    42301 gcctcgttga cggcggccac ccgtcgcggc tggcaggcga ttccttggca ggtttggcgc
    42361 acggcggcca gtttggtggt gttggtggca gtttgggtgg gcgccgccca cgcttgcgcg
    42421 cgcatcaagg cccgctccgc gtccgcccca gtggtggccc ggccgctggt ggatagttgc
    42481 cgcgccgcac cagagcctta tttggactaa cgcgaatgaa tttgcccaac acattgacgg
    42541 tcagccggct gattgccgcc gcggtggtga tgttggccat ggctttgccc attccgtttt
    42601 cgaccacgtt ggcatttgtg gtctttgtgg tggccagcat tacggattat tgggatggcc
    42661 ggcttgcccg cacccactat ggcgtgaccg cgttcggcaa gttgatggat ccgctggcgg
    42721 acaaagtgct ggtctgcgca gcgctggtca gctttgtggg cgtacgcctg aaattcgagc
    42781 ccaattactc gctcgtgccg gcatgggtgg tggtggtgat catcgcgcgc gaatttgccg
    42841 tgacgggcct gcgcctgctg gcggcggcca ccgatcagaa gcgcatcatc agcgccggca
    42901 gttggggcaa gtggaaaacg gcttggcaaa tgaccgccat tattgccacg ttcattttgt
    42961 tggccgtgcg ggaggacctg atgccctacc tgcacatgtc caagaccttc ctgcgccagt
    43021 acgatgccgc gtttgtcatc acttcgtggg tgctttcaac gctggtcgtg ctggtgacgg
    43081 tgatctcggg ctggaaatat tttgcggatc actgggattt ggtgacagcg gaaatgtaac
    43141 ggcggccacc gccgccaaag ggagacgaaa tcatgaagca aatgtggaaa tggatcttga
    43201 gcgtggcggt gctgcccgcg ggcacggctt gggcgcaaac ggaaccggcg gccggcttga
    43261 ccttggccga cctgatgcgc atgggcggct ggctcatgta tgtgctcggt gcgctgtccg
    43321 tgctggggtt ggccatggtg gtctattacg ccttggtgct gcgtccgcgc tacattgccc
    43381 ccaccgcgca agccctgcgc ctgcgtgaac tgctcaaggc ccgccggctc agcgatgccc
    43441 gcgcggtatg ctcccagcaa cccacggcgc tggccgccgt gacggccgcc ggcctcgatt
    43501 tcctgaagga aaatccgaac ggcgcggatg ccatgctcaa ggaaatcatg gaaagcgaag
    43561 gcgcccgcca ggccgaccgg ctgcaaaaca tgatccacta cctcgtggat ttgtcggccg
    43621 tggcgccgat ggtcggtttg ctcggcaccg tgattggctt gctcaaggcg ttcaacaacg
    43681 tggcgtttga tctcgccaag gcccgcccga tggaactggc cagcggggtg gggcaggcgc
    43741 tcatcactac cattgccggc ttgatcatcg ccattcccgc cgtcatggcc tattcatggt
    43801 tccgcgggcg ggtcatcaag ttgaccggcc agttggaaga atccgccacg gcgctgctgg
    43861 cggtgctgga agccaaaggg gaataaatga acttccgcaa gaaattgcca actcccggca
    43921 acttgggcgt gccgctgacg ccgatgattg acgtggtgtt cctgctgctg tgcttctttg
    43981 tcaccagcca gatttttgcg cagtgggaaa ccgaaattga catcgcgctg cccaccgccg
    44041 ccaccggcga catgccccag cggctgcccg gcgaggtgat catcaatgtg ctggccgatg
    44101 gcaccgctgt ggtgaatggc cagacgttgg acgatgccgc gctgcgggcc atgatggacc
    44161 ggcttgttca actgttcccc ggccagcccg tgctgctgcg cgcggacaaa tccaccgcct
    44221 acgaacacgt ggtgcgcgtg ctggatacct gccggcaggc cgatatctgg aacatttcat
    44281 tcgccacgct ggcgccaggt tcttgatgaa gcgactggtt cccgttttgc tgttgggcat
    44341 cgtgctggct acccgcgtgt gggcgctcag cgccgatgaa cagatgcgct ttgccgacgg
    44401 catctacctg cgcggctttt acgaaaccgc cctcaccgag tatatcgcgc tgatccgcga
    44461 caaccccggc agcccgcacg aggtggccgc gctgtaccgc attggcgagt gctaccgcca
    44521 attgggcaac caagccggcg ccgagcgctt ttacaaacgt gttgcgcaag agttctcgca
    44581 aagcccccaa gcgccccgcg cggaattgcg ccgcgcggaa atcgcgattg ccgatcagca
    44641 gtacgccgcg gcacaagcca tcctcaccac gttgcaaacc cgcgatgcca cgccggaaac
    44701 ggctgctgcc gccggctatt atctgggcta tagccggctg aagaacaacg acgcccccgg
    44761 cgcggatgcc gcgttccggc aggtgttggc cgattttgcc gactcgcctt atgcatccta
    44821 tgccgcgctg gatttggccg cgctgcacgc caaggataaa acgcctgcca cgcaaatgac
    44881 agcgtggtac gagcaggcgg tggctgcggc acctacgcct gccgccaagg cggaagccct
    44941 tttccgctgg ggcgactggg cctaccgcaa tgaagattat cagcaggctg ccgataccct
    45001 ccaggccttg ctgctggaac tgcccgatca gccgcgagcg cgcgacgcct ggctcgccgc
    45061 cggctggagc ctctatttcc tcgaacgcac accggaggcc atggaactgg ccgaaaagct
    45121 ggcgcacgac gcgcccgatg ccaatctcgc cgcggccggg gtctatctgc gggccaactg
    45181 cctgcgcaag ttgaatcgcg atggcgaagc gctggttgat tacgaaaccg ttgtccgcga
    45241 ttatccgcgc accgattacg cggcccgcgc agcctacgaa atcatggtca cccgcttcaa
    45301 gcgcggcgaa tacgacaagg ttctcgttgt cgcgcccgcg cagccggcca aggagcaggc
    45361 cgccgacgtg ctgtggatgc gcgccgaagc cgagcgcaac ttgggtcggc aggatgatgc
    45421 ccgcggccat tacgaggcgc tggtgaagga atttccacgc gccacccaag ccgtgccggc
    45481 gctgttgcgc ctgggcgaaa tggcccgcgc cgccggacgc caaagcgagg ccgccgcctg
    45541 gttccaacgc gtggcgcagg attatcccaa aaacgaggcg gcggccgaag ccctcaaggc
    45601 ggctgctttg gcgaatctgc gagcgggaga tcccaaagcc gcgcttgcgg gatgggatga
    45661 ccttctcgcg cgctcgccta ccggtaccgc cgcggccgaa gcccgcttgc aaaaggcgct
    45721 ggtgttgatc gaactcaaaa aaccgaagga cgcgctggcc ttgctggatg cgctggtggc
    45781 ggatgcgccg gcgggcgcct ccattgccgc cgctcaatac tggcgtggcg tactgctggc
    45841 cgagcaggaa aaatggagcg cggcggaagc cgccctgcgc gcttgccttg ccgccgcgcc
    45901 ggagccgcaa accgcctcgc tggcgcgact gcgtttggcc gtggcgttgc aacgcttgca
    45961 gcggctcgac gaggcggccg accaactggc tccgctgctg gcgcagccgg acatggtggc
    46021 cgacaacccg gcgctaatcg aatggctcat tcgccgcagc tttgagcaag gtgacgacgc
    46081 gcgtgcgctg gaagccgcaa cggcgctggc ccaacacgcc cgcgaagcca gttggcggca
    46141 aatcggctgg cattgggtcg gcaccagcca ggcgcagctc ggccatgaaa aggaagcggt
    46201 ggccagttac cgcaaggcgg tggcggaacc agcccacacc cgcgaaggca ccgaagccca
    46261 attgctgctg gccgaactcg aactcaaaaa tggcgccctt gaaaaggccg aggccgcctt
    46321 taccgctgct ggcgaggccg ccgccggcga ggacaccttg gatttgcgcg cgcgcgccta
    46381 tttcggcttg gccgagaccg ccgaagccgc cggccaattc gacaaggcgg cccgccactt
    46441 catgagcgtg gccgtgctct atgacgatcc ccaatggacg ccgcaaagcc tgtatcgcgc
    46501 cagccagctt ttcggcaaac tgggcaagaa agcggagcag gagagcaccc gccgcgagtt
    46561 gttgcagcgc tatccggatt ccagtttcgc ccgccaactg caagaggccg cgccgtgaat
    46621 tcggagtgga acatccgcgc ctgtgccgac caatgcatgc ggtgccagcg caagtttgcc
    46681 gacaaggaaa acctgatgtc gcggctgtgc ttcgccccgg aaggctactt gcgcgaagat
    46741 ttttgcggcg gttgctggcc ggagcgcgcc acgcccaccg ccgccgcgga agtgagcgcg
    46801 tgggccgccg tctggcacgc cccggcccca aaagcgccgg agcccctgaa aaaagaaacg
    46861 gccgaatcgc tcctgcgcga gctgatggaa accgacgacc ccgccaagcg caacgtgatt
    46921 tttattttgg cggtgatgct ggaacgccgc cggattctgg tggaaaagga agtccagact
    46981 cccgaaggtt ccggcacgaa gatccgggtc tatgaacaca agcaaaccgg cgaaagtttt
    47041 gtggtgccgg acccccaact gcgcttgagc gagattgagc acgttcagcg cgaagtcatg
    47101 gaactgctcg ggattgcggc gccatcgtca tgagtccggc gcgcccaaag cacttcatgg
    47161 cccttattcg ccgcggcgca tggctcctgg ccttggtgag cttgccgctg gtggcgctgg
    47221 cggagaaaac gccgcccggc ccgccgtggg atgatccacc gccggaactt tggttccccg
    47281 tcggcgaggc cattgattat gaagtgtttt ggggtgtgtt catggtgggc gaggccaccg
    47341 cgcaggccca gtggcgcgaa caggatggcc ggcgcttgct ggcgctgacc gtggatgcgc
    47401 aatccaacgg catcgtggaa aaactctatc ccgtcaaaga gcaccttgaa accctgttgg
    47461 accctgtcac cttcctgccg atttcctttg agaaagaaag ccaggaaggc cgtcgctatt
    47521 accacgaaat caccacgttt gaccacgcca cggggcaggg atattggcgc tcgttgcgct
    47581 ccggcaagga aaaggaattt gccatcgcgg acgacacccg cgacctgctc gggttgatgt
    47641 actggatccg caaagacccg atccgggccg gtgaaacgcg cgaatttccc ggtgtcatga
    47701 cggatgaaaa actgtacgag gtcatcgtcg aagccggcaa agccgaacgc gtggcgctgt
    47761 ccgaatatgg caaggtgcag tgcatcaaga tggagcccaa gggcaagttc aacggaatgt
    47821 tcgtgcgcaa gggccggatg tggctgtggg tgtccgatga tccgcgctac accatttgcc
    47881 gtgtggcggc cagcgtgccc gtggccagca tcaaaatcat gctgaagcgc gtgcgcgggc
    47941 cgggggatga tttttgggtt caggcgggag ataacaagtc cgggaggaaa aaatgagaat
    48001 atccgcaaaa cgcgcggcgt tgtggctgcg gaaatttccc cgtcgccggg tgttggtggt
    48061 cggtgatttg atgctggacc gctatatcta cggcgcggtc tcgcgaatct cgccggaagc
    48121 gccggtgccc gtggtgcgcg tcacgcgcga agccagcatg cccggcggcg cgagcaatgt
    48181 ggcggtgaac atccgtgcgc tgggtggcaa ggcggccgtg gccggaatgc tgggcaagga
    48241 tgccgccggc gtggagctgc gctggctgct gacccatgcc gaggtggatg tggaatgcgc
    48301 catgcttttt gccggcgcca gcaccatcat caaggagcgc attatcgccg agcgccagca
    48361 agtggtgcgc gtggacttcg agcggcaggt gccctggacc gaccgccaga ccgaaaagtt
    48421 tttggcgatg ctggctgtcg aactcgcccg cgtggatggc gtgattattg aggactacga
    48481 caagggtgtc atcacgcagc cggtggtgga cttggtgttg cagatcgcgg gcaagcgtgg
    48541 cattcccgtg gggttggacc ccaaggaagg ccaccagctc gacattcagg gcatcacggt
    48601 ggccacgccc aaccgcaagg aagcgtttgg catcgcgggg ctgctggacc ccggcgccaa
    48661 ggaaaacccg ctgaccgaca aggcgctgct caaggtcggc cagattctca tggaaaaatg
    48721 ggccgcgcaa aacctggtga ttaccctcgg cgcgcagggg atgttcctga tgtcgcgcgg
    48781 caaggcgccg cgccatgtgc cgacccgcgc gcgcgaagtg tttgatgtca gcggcgcggg
    48841 cgatacggtc attgccacct gcgtgacggc gctggccgcc ggcgcggatt tccttgccgc
    48901 ggccgagttg gcgaatattg ccgccggcgt ggtggtaggc aaactgggta ccgcctcctg
    48961 ctcgccgcag gaattactgg cccacatgaa gcagccggcc acatgagtgc gcgcgttgtc
    49021 ggtgtgattc ccgcgcgctg gggctccacg cggttccccg gcaaaagtct cgccatggtg
    49081 ggcggcaagc cgctgctggc ttgggtggtg gagcgcacca agcaggcccg ccgcctggcc
    49141 gagattctcg tggcgacgga tgacgaacgc atcgccgccg ccgcgcgcgc gctggatgtc
    49201 cgcgtggcca tgacccgcgc cgaccatccg tccggcaccg accgcatcgc ggaggccatt
    49261 cgggagctgg acgcgcccca cgtcatcaat atccagggtg atgagccgct gattgatccc
    49321 gcgctgattg accgcattgc cgccacgctc gccgacaatt cgcgctggga catggccact
    49381 gccgcggcgc ccgtccacag cgccaaggag ctgcataatc ccgatgtggt gaaggtggtg
    49441 cgcgatgccg ccggccgcgc gctctatttt tcgcgcgccg taattccctt cgtgcgcggc
    49501 gcggcgccgg actttgcgcc cgtgcccggc cagcattggc ggcatatcgg catctatggc
    49561 taccagcgcg ccttcctcga acgattggtg cgggaaccgc ccggcgcgct ggagaatctg
    49621 gaaaagctgg agcaattgcg tgcgctggcc atcggctgcc gcatggccgt tctggaaacc
    49681 gaccatgtcg gcattggcgt ggattcaccc gccgatgttc cccaggccga ggcgctcctg
    49741 cgccaagccg gcctcctccg gtaacgccgt gcgtttgccg gcccggcccc gcgccaaac
//
LOCUS       DAOPNP010000004        48754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_935_length_48754_cov_9.677406, whole genome shotgun sequence.
ACCESSION   DAOPNP010000004 DAOPNP010000000
VERSION     DAOPNP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 48754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48754
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_48754_cov_9.677406"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..854)
                     /locus_tag="PKI64_00820"
     CDS             complement(363..854)
                     /locus_tag="PKI64_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD99580.1"
                     /translation="MTHRITLTVNGIQRTLHVEAADTLLRVLREELHLTGAKKGCGEG
                     ECGACTILMNNQAIRACLVLAVEADGADIVTIEGLAPPGEITRVQKAFIEADAVQCGF
                     CTPGFVMALEGEMRRNPHATRDDLLNALGGHLCRCTGYESILQATDTLARQLAQKTKK
                     SKK"
     gene            complement(851..1693)
                     /locus_tag="PKI64_00825"
     CDS             complement(851..1693)
                     /locus_tag="PKI64_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOD99581.1"
                     /translation="MNLEYCKPQTLEDLLAETRAGTHLMAGGTDLLVDMRSRKKTVQR
                     IVDIKAIPELRGIRETKSGLEIGACETFTALRESPLVAAWSPALRECADVMGCHEIRH
                     RATLGGNICNSSPGCESGVVLSVHEAQVRIASTRGVRLVPFDTFATGVNRTCLEPDEV
                     VLSIILPKAADSLSFYRRITRTAGMDLACWNGAALVLQPRIPAKRVVRISFGTVMPTP
                     YRPRAAEALLSRRPLTPADMDQAYDLIIREIAPRAGSVRASPAEKKRAIRNFLDELLE
                     RLAP"
     gene            complement(1690..4011)
                     /locus_tag="PKI64_00830"
     CDS             complement(1690..4011)
                     /locus_tag="PKI64_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOD99582.1"
                     /translation="MKQVGRSVVRADALEKVTGQAIYVDDLQFPGMLHGRVLRSPYAH
                     AKIVSIDTSAARQAPGVRAVVTGRREEQDVGGGKLPLVGACIVDQLPLAMDRVRFAGE
                     AVAVVVADTVAAAEHATTLIKVEYAELPVLTDARQAMAKDAPLIHPDLGSYECAKGFH
                     PKPGTNIFHHYQLRKGDTRKAFAKCDVVLEQEFSYPHMSHCQLEPHGALVRWDPAGSV
                     EIYSSTQAPFVVRGYLSRAFRLPLSKVRVRAPVVGGGFGGKSDYTLEPLAAYVARQVP
                     GVWIKLIYEREEMFNGTLVGRGVTGRYKLGAKRDGTVLAAQIEYILSGGGYGECAINV
                     AVGGGQNSPGAYHIPNIAVDSYAVYTNLPPVGAFRGYGHPEGQWMAERMMDLLARELK
                     MDPVELRLKNAYRAGDKNAIAQRLHPTQGDLPQCLREAAKAVGWKMGQSNVVKTRTGF
                     RTKAVCSLMKSPVQATNAQVAAILKFDEDGGVNLIITTCDMGQGSATVYGQIAAEALG
                     LPLERVHVVRDPDTALFPADWQTIGSKGTYTGGIAIVRAAEDAIAQIRATAAKALRVP
                     VAKVRYQDGRVVIGKRHHDIGLFTNGYMLADGRTIGRPVVGYGAYTPDLQIPGIRKPG
                     LQIGQGNVSASWTFGAQAVELEVNPKTGQIKVLQLVTAIDAGRIINPRLARGQVVGGM
                     VQALGQTLMETMIYSDKGVLRNGRFTDYKIPALEDLRDTDFKVIFVETPDRNGPFGAR
                     GLAEHGTVAIAPCVANAIARATGQDLYSLPIRL"
     gene            4198..5367
                     /locus_tag="PKI64_00835"
     CDS             4198..5367
                     /locus_tag="PKI64_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOD99583.1"
                     /translation="MKPRKIREGVHWMGAIDWNRRLFDSLIPLPDGTSYNAYLVEGAE
                     KVALIDTVDPPLLDVLKRHLANVPRVDYVIAHHAEQDHAGSIPYVLEKYPNAIVICSP
                     KAKDLLLTHLDVPENKIKTVEDGETLALGGKTLTFIHTPWVHWPETMSSYLKEDKILF
                     SCDFLGSHIATSELYAGDDRYVIEAAKRYYAEIMMPFRAMVRTNLKKIRELDFDLVAP
                     SHGPIYDKPEMILAAYEDWSSDRVENRVVIPYVSMHGSTELMVDHLVSALAERGVAVQ
                     KFELTTTDIGKLAMALVEAATIVVGAPTVHVGPHPAVFSATHLANALRPKLKYAAIIG
                     SYGWNTQVIERIAGLIPNLKVEVLGTVLSKGVPRAATFAELDALADTIRDKHATL"
     gene            5354..5713
                     /locus_tag="PKI64_00840"
     CDS             5354..5713
                     /locus_tag="PKI64_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOD99584.1"
                     /translation="MPRSKPKIAIQRIYDTPVDGYRVLVDRLWPRGVSRDAARLDEWL
                     RDLAPSAKLRQWFNHEPARWEKFRAQYRAELAPQQETLARLRDIAQRKKLVLLYAAKN
                     EDYNNARVIQEILNESS"
     gene            5850..8282
                     /locus_tag="PKI64_00845"
     CDS             5850..8282
                     /locus_tag="PKI64_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD99585.1"
                     /translation="MKTIIIGGVAAGASTAARLRRMDEKAEIVLLERGPHISYANCGL
                     PYHLGKIIPERSWLLVMTPEDFRARFNVDVRVLHEVTAIDRAQKTVRILNRKTGVENE
                     ERYDKLVIATGSSPMALQLPGMDLPEVLPLWTLEDMDRIAKKLDAGAKHVIVVGGGFV
                     GMELAESLRHRGLGVTLLEMGRQLLPTMDEEMSSLLAEELRLAGIVVELGAAVSAFAA
                     APGGGVTATIKDGRSWTADLVALCVGVKPNSQLASAAGLAVGPKGHIITDAAMRTADP
                     DIFALGDVVEVMDPVFGTPTAVPLAGPANRQGRIVADNLLGGSRRYEGTFGASVVKVG
                     KLTAASCGHTEARLKAMGKTYQKVYVHAGSHAGYYPGATTLHIKLLFGNDGQIYGGQV
                     IGAEGADKRTDVLATAMRAGMTVRQLAELELCYAPPFSSAKDPITVAGMIATNALDGA
                     TRLAHADALPEGALLLDVREPAELPQGALAGSVNIPLHKLRSRLFELPKDRLIVVYCQ
                     VGLRGYVAERILRQLGYDVANLSGGYLTWKQFQPGMWMPTDAQIALATTGSEGGPRLE
                     LEEEEPATVVDVRPLQCPGPVVRIKQELDKLGDGATIKVLAQSSFAPDLTNWANSSGH
                     KVCKITPTPTHLEAILRKGAGPKPAVGIAAPQSGAMVLFSNDLDKALAALIIATGMAA
                     TGMQVSIFFTFWGLSVLRKHPGPQVKKSFLSTMFGWMLPRGAQKLALSKLHMAGAGTA
                     LMKHVMAQQNVASLPEMLHQARALGVKFIACDMAMGVMGLTREELIEVDEVAGVATFI
                     ARAKESGPTLFI"
     gene            8506..9192
                     /locus_tag="PKI64_00850"
     CDS             8506..9192
                     /locus_tag="PKI64_00850"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD99586.1"
                     /translation="MSMNMTGLIGLAAIGLAAAASGQGAPALVFEPLPYAYDALEPYI
                     DAQTMEIHYSKHHQAYFNNLVKAVAGTAMEGKAVEALFPEISKLPVAVRNNGGGHWNH
                     TFFWNSMAPNAGGTPEGALLAAIEKSFGSFDEFKKQFEAAAVGRFGSGWAWLAVDANG
                     SLFVSSTPNQDNPLMDVVEKRGTPILGVDVWEHAYYLKYQNLRASYVSAFWNVVNWPE
                     VAKRYAATQQ"
     gene            complement(9263..9976)
                     /locus_tag="PKI64_00855"
     CDS             complement(9263..9976)
                     /locus_tag="PKI64_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99587.1"
                     /translation="MIPLPTAADEAELLRDHLAALLCDARLDTTAAEAVAAAVTAYLD
                     DQQLTLPLPDDYLHLLIYRALRTLNQNDTARLWAQTHHLGNGDVPIFDPEIWPGPVPL
                     IVWQLFASRLVRPARTITAEGRILWALDFRRLRQEQAGWLEITLLPGLRALLEQLAPA
                     WDESSGAGLLGLRGLYAAGFAPTPRRRRKKAKTYSAYAPLDAAAIRSFCLRVLANLQQ
                     PRGWSTTPDVLYLDFSRKG"
     gene            complement(10199..10654)
                     /gene="nrdR"
                     /locus_tag="PKI64_00860"
     CDS             complement(10199..10654)
                     /gene="nrdR"
                     /locus_tag="PKI64_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOD99588.1"
                     /translation="MRCPKCAQDNDKVIDSRAIRNGAVVRRRRVCAHCGHRFTTYEEV
                     IKTVLTVLKRDGRHEEFSRAKLRAAIVHAAVKRPISDPQIDLLVDDIIHEISAEYERE
                     VPSTVIGRKVMDKLERLDEVAFLRYASVYRRFRDASQFLSEIENLIGRQ"
     gene            10764..11579
                     /locus_tag="PKI64_00865"
     CDS             10764..11579
                     /locus_tag="PKI64_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD99589.1"
                     /translation="MDERDDQGMRIYLREIGKVELLSPEEEIELARRIKKGDEQARQK
                     MIRANLRLVVKIAGDYARLGLPLLDLISEGNIGLMKAVTRFDPKKGGKLSTYAAWWIK
                     QGIRRALANQSKTIRLPSHMVDKLFRMRRTYEAMTHELGRQPTAQEVALRMDVPVQRV
                     KYWLEVSQRVTSLDEPIGDEGRESQSDLVSDPDARSPFDDLNDRQLLDEMLEHLKVLN
                     ERERTILERRFGLNGKEPESLEEVGKKLGITRERVRQLQNQALATLREMMLEH"
     gene            11628..12002
                     /locus_tag="PKI64_00870"
     CDS             11628..12002
                     /locus_tag="PKI64_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99590.1"
                     /translation="MAEQNGNEIQMNAANLYREENYTDLRAGSLRKLIPVKPDGTDDP
                     SRPVIFTAATQVMTPGGILPLSGEVPNAQTLAEAVAGFAPAIKQSLADLREEMAALQR
                     ERASQIVVPGRDVPPPSDLLIR"
     gene            complement(11999..12508)
                     /locus_tag="PKI64_00875"
     CDS             complement(11999..12508)
                     /locus_tag="PKI64_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOD99591.1"
                     /translation="MGAGLGKVAGMNPVVAKIAELLRARGWLLTTAESCTGGLVSGEL
                     TALPGSSAWFVGGVVAYANSLKAGLLGVTPETLAAHGAVSAETAAAMARGACAATGAD
                     CAIAITGIAGPTGGTAEKPVGLVFIAIATPAVSRVFEHHFGGTREDIRAAAVAAALEH
                     LREALKDRI"
     gene            12667..12927
                     /locus_tag="PKI64_00880"
     CDS             12667..12927
                     /locus_tag="PKI64_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOD99592.1"
                     /translation="MSKTAMVRARVQPELKTSAEKVFHRLGLNATQAITIFYKQVELR
                     DGLPFEVVTPTSTTKQTLADSEAGRNLVVCENADDMFRQLGI"
     gene            12927..13199
                     /locus_tag="PKI64_00885"
     CDS             12927..13199
                     /locus_tag="PKI64_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOD99593.1"
                     /translation="MYTPVFTKQFAKDIKRCQRRGKEMEKFKLLAHTLLAGRQLDPIH
                     RDHKLTGSMTGRRDCHIESDWILIYRIAADQLIFERMGTHSDLFKK"
     gene            13348..14418
                     /locus_tag="PKI64_00890"
     CDS             13348..14418
                     /locus_tag="PKI64_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD99594.1"
                     /translation="MKTVMVTGSGGLVGAETARHFAAQGCRVLGLDNNMREYFFGPEA
                     STREVVAELQAAYPEEYVPLAVDIRDEATVRRIFTEAKGLELVVHAAGQPSHDWAARE
                     PFTDFGVNATGTLNLLEATRQYAPEATFVFTSTNKVYGDTPNRLPLVEQATRWECVEG
                     PYAANGIDETMSVDQTTHSLFGASKLAADVMVQEYGRYFGLRTGVFRGGCLTGPRQAG
                     AELHGFLAYLMKCAVTGREYRILGHKGKQVRDILHCADLVAAFVAFHRAPRAGAVYNM
                     GGGRFSHCSLLEAVTLCEELTGRHMKTSYVATPRVGDHIWWVSDVTRFQADYPEWQVT
                     RNVRDILAEILEYHRARWTRDG"
     gene            14423..15193
                     /gene="nudC"
                     /locus_tag="PKI64_00895"
     CDS             14423..15193
                     /gene="nudC"
                     /locus_tag="PKI64_00895"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOD99595.1"
                     /translation="MAESLTFIFCDGLLLVRSGSWQPAGDDVLAELLTTRQLAAEYRV
                     PPQGHWAKQVSGATDNAPPGYEWVRLRTLLGADVPYALAACRALGMLNWRATHRFCGR
                     CGGVLAEHHVEVARQCAACGHVEYPGPTPAVIVRVEKAGQILLARHVQRIPHLWTCLA
                     GYVELGESLEDAVHREVREEVGLEVGDVQYVASQHWPYPNQLMVGFRAQWRAGDLKLQ
                     PEELSEARWFDPAHLPDIPPKGTMAYRLICETPAPMMG"
     gene            complement(15384..16013)
                     /locus_tag="PKI64_00900"
     CDS             complement(15384..16013)
                     /locus_tag="PKI64_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99596.1"
                     /translation="MNIEARVTGFIEENCYFVTNPATRETVVIDPGDDAAQLLVRLRE
                     LGGTVIGYLLTHGHVDHVTALAALTAAHPAPVYLHARDADWCFTERNSLPGIYPAPTK
                     PDVPLVFVKEDDTFQLAGATWRVLETPGHTPGGVCWHLPAANALFTGDTLFRGSVGRT
                     DLPGGNSRVLTQSLRKLAALPEDLAIYPGHGEPSTIGREKRDNYFMQFR"
     gene            16017..16994
                     /locus_tag="PKI64_00905"
     CDS             16017..16994
                     /locus_tag="PKI64_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99597.1"
                     /translation="MTATDSSTGVKDAAEIVLAVSGLGVGYYPRVGERWDVVAEVNLN
                     LRAGRTVALVGESGCGKSITAAALGGLLPRNMQRDRGELRWAAGVDARPGRGLAYVFQ
                     DPGECLNPVFTIGNQIREVLPPEDVPDASARLTSLLRTVGFTESERVLASYPHQLSGG
                     MQQRAMLAMALAGRPRVLVADEPTTALDVTVQAKILQLLGQLQRQENLAILLITHNLG
                     VVAEMADEVHVMYAGRIVESGPVALLRNPQHPYVQGLLRALPRLHGEAGRLVGIPGTV
                     PPLGRRPAGCGFHPRCALGREICRTQEPPWRENQGHGVACWTRGAGSWA"
     gene            16991..17785
                     /locus_tag="PKI64_00910"
     CDS             16991..17785
                     /locus_tag="PKI64_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99598.1"
                     /translation="MSTPLLTVDNLRVTYRVGRRRVAAVKGVSFSVARGQCLGIVGES
                     GSGKSSLARAILALEPAATGQVIFAGEDVLKMSRAARQVFRRRAQMVFQDPLGSLNPR
                     LKIGAALAEVLRVHGLARNRREAAAQTAAWLATVGLDAEYATRFPHELSGGQRQRVNL
                     ARALCVGAEFLVADEPVSALDVSVQAQILNLLKTLQTERGITWVLIGHDLAVMRHMAD
                     ELVVMQAGEVVEHGRAVDILASPQHPYTRELVAAVPDVDRALQCRA"
     gene            17853..19118
                     /locus_tag="PKI64_00915"
     CDS             17853..19118
                     /locus_tag="PKI64_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD99599.1"
                     /translation="MYGVKQLWLARLKKIAGPDGHWLALAGVALLWLTPTKGYVPLIL
                     WVAGLYGLWNWRKLAAAWNNVAGWCFALGLVWAVASVAWSFYPMGSGRDLVKSLPMVA
                     ATMALPAIFDRPTRIWNALLVSAGVVSLKLALDLWRLWAALGWSALLTESRFYHPYLY
                     NHPNVSSMMAGLAALVFGVRLLAGGQHRKWRALLVLGLLVDLLYLEVLASRGPQLVFA
                     LVVLSLPLALLPSKRVRVVAALLAAAIGVGLWCAAGSINPRFEDRTMKHYNDRDTIWG
                     HSWLLVTKRPIVGYGFGKKAFEKAVYENPELRGPRVRFHYPHAHSYWLMVPFQGGFVA
                     LALWGAGWLALGGRLAYRMCRWTAQAPPAWRARVQVRLLPALLGAGVMFILVYGIGDY
                     PDNAIRHAQFYLAGLIMALTRPAVPEADA"
     gene            19115..19939
                     /locus_tag="PKI64_00920"
     CDS             19115..19939
                     /locus_tag="PKI64_00920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD99600.1"
                     /translation="MKISVLTTIYNRPEHLRLLVAALQAQTRRPDELIVADDGSNAET
                     VAAMTRLLPACGIAARVVRQEKDGYRLAAARNMAIRAATGDYLVFLDCDIVPLPDTLA
                     VHERLAESRRLLCGHRALLDEIATEALFAAMPAPAEADWELAWAVADKTELQEAARLF
                     ARHAALRRWHLARAHKPKLIGCHFSLFRDDVFRVNGFDENFVGWGYEDDDFARRLYKS
                     GVSPLGVLEDARALHLWHPTLAPSPLARRRDRPNRAYFRRWRVPAYCANGLRKPET"
     gene            19939..21147
                     /locus_tag="PKI64_00925"
     CDS             19939..21147
                     /locus_tag="PKI64_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOD99601.1"
                     /translation="MAWQEPTRLVITCGRGCVPALSGELCALGHRPQEVSPTAVEVTG
                     NLHEAMRLNLWLRTANRVLFEVAGFAVESPDELYAATRDLPWEEWLRGDVPFHVHGVA
                     QHETIRDSRFALLRCKDAIADRFVEQTGARPDSSASPVGAASIALYWKDGVAQLFLDT
                     SGVPLSHRGYRQQPWVAPLRETLAASILLETGWGQNPDEAFVGPMCGSGTLAIEAAWI
                     CQNRAPGLYREDFAFLLWNGVEADEWRQMRAAAQRQCRTAPRAWLAASDIAPEAVACA
                     RANAERAGVAHLIRFQVGDFRDAALPQPPALILMNPGYGERVGELAELYPLYEAIGTF
                     FKTKCQELRAAVFTGSPALGRKIGLHPARRTTMWNGDIECRLLEFEIYAGTRDPRLLR
                     KHRGESSAPQ"
     gene            21190..22173
                     /locus_tag="PKI64_00930"
     CDS             21190..22173
                     /locus_tag="PKI64_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD99602.1"
                     /translation="MKWSVPILCYRNISPVCGITPEEFRTHLEWLAAHGWHSIPLDQV
                     IDYIKGGAEPPQLSYALTFDDCYLDNWVHAAPILREYGVKASFGCVTAYLHDGPRRPD
                     ATTPGADLRELPVGRDAWERAIEHNDPTAFMNRAELRALVSEEGHSLFGHTHTHQACF
                     RSDKPLAAGADSLQSGTRGIYLEMRDGQPMFPSGSAYAHNGFWPTSNDLLDTSLVART
                     TSERIEFCLNEFTLCRERLEELLGQPSRAMSWPWGEYDSVAIEAALLAGYEVALSMDC
                     GANQPGTPLFALRRVKMQGGVGVRELNRRLCIAKNPLLAGLFRKIYRVPRG"
     gene            complement(22198..24177)
                     /locus_tag="PKI64_00935"
     CDS             complement(22198..24177)
                     /locus_tag="PKI64_00935"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOD99603.1"
                     /translation="MKKFGFFLLWLVGVAVFLLATAHFTLRHLLNTPDFKKTVTEFIT
                     RATGRAADYGRIDYQLFPFALRVQNAVLKEADGQQDFASIQSFTAEVDFRRKEVTSVT
                     LDRPVVRIVQQADGSYNFTDIGRGKPATTKERSAAESETGAEPTSAAAAPLLVIGRID
                     LRQARVEFIRRATNGAEHKLVLSAMDGELTDFATANPLHFSGRMAIGEHSTLEFALSG
                     PPPADHSAQPIAWPLDLTARMEIGDVADIQALLPEAEFPFSGLTVALHAQGSLADRLA
                     VEVTADTPITAPGMPSAIHAKLTADLLLPAPVADHLLLGAALPDAFKYNPPPCAAPPG
                     TVALGESPLLALLLRHLQAQAQLTFTDLTLGANALGDGAANLLLHEGVLTIPTFTFAA
                     HGGRVTIRGAVQLLACPLAYQLTSLQADGLMLHNLLAANMEYAPPGLDGTVQLTASAH
                     GHAVGEEALPSLVADLDLQLTDLRNVGEGGSLADQMWRQLDQPLLLRLLPTLKAKVAA
                     AKQTANLVTTNRYEDATAKLALRNGRIAVSSARLAAADYRLNLHGVILPFADQLDLQA
                     TVRASPEETAQLTGGKDLSAFLPYEDGGLQVPLFIRGPLRKPVVVPDMDTLLKNAAAG
                     LTSQSDGGNILDSLSASDRKNVEKGLAILNDLLQQ"
     gene            24287..26092
                     /gene="pepF"
                     /locus_tag="PKI64_00940"
     CDS             24287..26092
                     /gene="pepF"
                     /locus_tag="PKI64_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOD99604.1"
                     /translation="MKPTPEPTAVPARADIPVELTWDLTPLYATPSAWEADFARLDEL
                     AAPVLALQGKLDSVAAVAQLLAAETALDRVLERLHTYAHLRHDEDTANTDNQTREARI
                     RARYAELAAQCAWITPELLMHPEADLRAWRDAPELKDYRRTLEKVLRNKPHVLSTAEE
                     TLLAGAAEVLAAPEQTFSLLTNADMTFPDIPGPDGPLPLTEGTYRAHLENADRAVRRA
                     AFETLLTGYGKLENTVAATLSSAVKAHVFMARARKFPSALEAALFDDQVPVAVYEGLL
                     AATHEALPLFQRYLDLRKRALRLPDLDMFDLYAPLVPSCATPVPMAQAREWLLAACRP
                     LGEEYGRVLERAFAERWVDWRENKGKRSGAYSSGCYDSPPYLLLNYHDRLDDAFTLAH
                     ELGHSLHTWLANQAQPPRTAHYPIFLAEIASTVNELLLTHHLLAQPGEPAFRAHLLNH
                     LCESFKGTVFRQTMFAEFERDLYRWEEAGTPLTAGFLREQYYELNKKYYSPAIAAHAA
                     IGWEWARIPHFYYDFYVYKYATSFCAALVFARRLRAGEGVAAYLDLLRAGGSADPLDL
                     VAAAGVDLREPRVLREAFADFGAVLTELEQALALP"
     gene            26089..26688
                     /locus_tag="PKI64_00945"
     CDS             26089..26688
                     /locus_tag="PKI64_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD99605.1"
                     /translation="MKARDAILTVLDFESTGAVSGHADEPWQVGMVEMQGGRVTGRHY
                     ESFLRVAASRPFNPYAPGRHAVLRPVLAAAPTPGELWPRWQLWLVGRPLAAHNVGTEK
                     KFVQRAAPLHEFGPWLDTLQLARRVRPDLAGHALGEVCHALGLVARLQQVCPGRDWHD
                     ALYDAFASALILEYCLALPGWEAVSIAALAAGPDAKSSC"
     assembly_gap    26765..26864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26997..27908)
                     /locus_tag="PKI64_00950"
                     /pseudo
     CDS             complement(26997..27908)
                     /locus_tag="PKI64_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252018.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28105..>29226
                     /locus_tag="PKI64_00955"
     CDS             28105..>29226
                     /locus_tag="PKI64_00955"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOD99606.1"
                     /translation="MSAKRKIAVLVGDGMGDEPVAVLGGKTPLQVAKIPTIRRVAGLG
                     RVQLVHTVPEGMSPGSDVANMGLLGYNASENYTGRAAIEAAGARLPLEAADVAYRTNL
                     VTVENGKMLDYSAGDISSAEAHELIAALNAAAARTGLKFYGGVSYRHLLIWRNGPAEV
                     TLMPPHEISGKPVADHLPRGPRAAEVLALMELSKPIFAEHPVNRARVAKGKRPATQIW
                     PWGFGKAMSLQKYPERYGLQGGVITAVDLVRGLAELCGLEVIPVAGATGWVDTNYAGK
                     AQAAIDCLRRSDFVYVHVEAPDECGHKGMAEEKVRAIENFDEKVVAPVWAALEAAGEP
                     YRLMVCTDHRTPLIKRGHTSDPVPFTWVDGPLGAAMQPDA"
     assembly_gap    29227..29326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29327..30083)
                     /locus_tag="PKI64_00960"
     CDS             complement(<29327..30083)
                     /locus_tag="PKI64_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99607.1"
                     /translation="MMVEREEHGCRVLTIVSPDTRVCADIVPELGGTVSSLKFAGREC
                     LFRHPWFWDPEETETRGGIPLLFPICGRLLHDGTPGRYLAQGQAHHLPIHGFAMRLPW
                     RVVGEPVLDSLRLRLTDTPATRAQYPFPFALEALYAATTRGLLIRLTVHNTGVVPLPF
                     YAGFHPYFLTPPPGAGKKRTRLLARPRRRHLYNDTKTAVTGNAPLPAFPLAITAEEAN
                     SLLLEMADNRETALLFPDGFVVRQRTSPTLPFRQ"
     gene            30149..30508
                     /gene="cutA"
                     /locus_tag="PKI64_00965"
     CDS             30149..30508
                     /gene="cutA"
                     /locus_tag="PKI64_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOD99608.1"
                     /translation="MNTDYGMVLTTFANETEAQALAAALLAERLAACVQMLPIQSAYR
                     WQGAIQREPEILALIKTRTALYPAVEAMIRARHSYDTPEIIFVPIAAGSPGYLQWLDE
                     STRADSPPSKSVGCQPS"
     gene            complement(30496..30954)
                     /locus_tag="PKI64_00970"
     CDS             complement(30496..30954)
                     /locus_tag="PKI64_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD99609.1"
                     /translation="MPIYEYQCEQCRKVFSFLVRNVATHQPPTCPGCGHGKMTRALSR
                     FAAHSGKRRAENADADPAAPSGDDMPPGMDRLMAEADGIDENDPRAMGRFMRKMAEET
                     GEPMPPEMNEMIRRLEAGEDPESIEADMGDMLDDGPGGGGGSGGDTLYDG"
     gene            31187..32980
                     /locus_tag="PKI64_00975"
     CDS             31187..32980
                     /locus_tag="PKI64_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD99610.1"
                     /translation="MKGKWLGFLGAAMCWVSAAIAAVPLPYLNKQTNAVDWTTLPGWS
                     GNVTNSSAGGSARFAAAGDTLTLALDGAPTTLTFTLRGYSNAVGVAPASFMVEESGDG
                     NVWNSTPVADIGEDSLSVAAAAFGPYELLSSTRYLRFTYVDHYAFDIGLNNVAVAGGP
                     AAPRVVFVDRPEGFIVAQGAENEVIQAVVVNGGGYWFGSEAQGKEPWESDNGGTALSE
                     SPYDKYYINTAATGTFFATANGRCDNTDEVVPGTIHFTVAPGYAVDLQVGDHGTATVA
                     VYGRAATNAPRGALVTILPVPDAGYATGVLQLNGAPIAGTTFIMPDGPAVVSVSFRKK
                     VAGDPTLIISQYYEGAGENKWIELYNAGAEPVDLGAAGYRLGVWQNAKRENWKTNAPP
                     AVATVLTGIIAPGETYLVCHVGTKAPAYAVANVVSNGMLFNGDDSVVLYTGTEYNLAN
                     VVDAFGMVGSNSVNCSFVRAVDVVSGVNTDFQSNQWVRFEYAAVDTAEVQVPERLGWH
                     TTATPQPTEPPKLDNLISTEGGLVFEIPTNYTDYTIYGAAELQSGAPAWAGSNIGDRC
                     SVELASNLWRITVPTTLGQRQMIWLSIPGGN"
     gene            32981..37057
                     /locus_tag="PKI64_00980"
     CDS             32981..37057
                     /locus_tag="PKI64_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOD99611.1"
                     /translation="MTTRGTRRPGGGLRVGLALLAFLLAAPLAQAQYGGAGSFVKITS
                     RADVADGYYVVANSDGTIAMSGTNPGAYFERSTVSPVNDTLTDPPAVNVWLLQTNSNY
                     GGFTIFNETSNKYVSFAGTANAAYAAGAVNGTTGVWSFAWRSDLGCFAVSNVARPYRY
                     LQYNTGSPRFSCYSNNASMQNLTLYKMLAATPTAPVFTSGSGPFNATALSATNFVVTA
                     SGQPAPTLALQGQTASGGFSFTAATGVLNYTPPVADIGTQTFTFTASNEVGVATQTMT
                     VVVSAAPVYVPEVSVTNVATNGFTVQWTACTGATGYQVQVATDMNFTQASSGETTNVL
                     TNAAGSASPPTGWTYNISSKSSSYLILLADTHEVRSPIFSTEGLTALSVDFLARTYGG
                     VQSGVNDVEVFISTDGGTNWVSLGTVRPTDSNLRQYSVAASAYVGYAQVCLRWTTPNA
                     TGAVGAGIKALNVTGSETGAFGSLVVDVTTSALATNITGLALDTAYYVRVRRLPVEEW
                     SAVVPVTTAGAVPTAPWFISSAGPYSVAAGDMVDFIVSALGVPTPVLALSAATAPGID
                     YEFDPNDGYFVYQPPLAASGSTQVFTFTASNTQGVATQTVTVNVATVTAPAFGANPGP
                     LVATVGVACVFTVSATGLPPPALALQSQTAGSGYNFTPATGELSYTPPAGDIGSRSFT
                     FTAGNVAGTATQVVQVTVHALPTIDPLPTISIAAGQTTNVWIVAREAEGDSMTLAASN
                     LPANAAFSTVSGTSVISNLFTFAPDSNQMGQAYTVAFTASDPHGSVIQTLSITVASAD
                     PWADYYASCYENGVLKTGNALKSALHDIISPHTSYSYDAAITILSEIDECPTNNTMVQ
                     LLYLQHGRAKSSFGGSNGQWNREHVWANSHGVNDKLPAYSDVHHLHPTDVQVNSTRGN
                     KDFDTVSGSVSNYSYNSTSFEPPNAAKGDVARALFYMAVRYDGTGGVSDLELVDSVGT
                     SGAKHGKLSTLLNWNELDPVNDYEIRRNNLVYSNWQGNRNPFVDHPEWARVVFDPNYV
                     PLASLTSFAATPNGSSQIDLSFAYTGSGDGVVIVWNETGSFSAPSGTAPAVGQSFAGG
                     TLLYKGAVSPQSHTGRTACQKVYYKCWTYTGTNYSAVGLTADATTAGPAAPASAWASA
                     TNTTDFTAAWSAVSGISQYLVDVVTGTTFGGAGSVWVPGYSNRAVSSATSLSVTGLTA
                     GATYYFRVAPVPNCDTRFSPTGTVTTLGETLTPFQQWLQNTRGQNINDPHFSPEADYD
                     GDGMTTWEEYLADTDPASAASVLALNGWFSVSDHRIRFSFPASTGRFYRLEYCTNLTN
                     TTGVAYSNLGWGTPGMVITNPGTSNAWFWILRASPNAP"
     gene            37109..37816
                     /locus_tag="PKI64_00985"
     CDS             37109..37816
                     /locus_tag="PKI64_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOD99612.1"
                     /translation="MQWPLLDMLFSRTCEGCGNDLATEPGALCWDCRVGLKPVQVPFC
                     ERCGDPVAGVTGGPFECTWCRQNQPAFVWARSAVRYDGIAAACIRRFKYHAGVWLQAE
                     LVDWLWALWRTCPEPKVADLVAPVPLYRRRERERGFNQAALLGRSLAKRMGIPFRGNL
                     LRRHKPTATQTHLTAAQRVHNVRGVFSAPAPRRLQGARVVLVDDVMTTGATVNECARV
                     LMAAGAQAVMILTVARG"
     gene            37853..38728
                     /gene="accD"
                     /locus_tag="PKI64_00990"
     CDS             37853..38728
                     /gene="accD"
                     /locus_tag="PKI64_00990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOD99613.1"
                     /translation="MTFFGKKKTVVKRARKRDMPDGLWLKCPACNEMIFGEEQEQALR
                     MCPLCHHHFPMSRNERLAMLADPGSFVEWDKDLYSIDFLGFVGVASYQEKLESNRRKT
                     GEVDAVTCGRARLNGRAIALGVMDFEFLGASMGSVVGEKITRLIERATAEKLPVIVIC
                     ASGGARMYEGMMSLMQMAKTSGALARHAEAGLPYIPVLTHPTTAGVMASFATLGDVII
                     AEPGALIGFAGARVIKETIQQDLPPGFQRPDFLLKHGLLDQIVPRNELKATLARLLDA
                     FCGVLQPSEPPEIPA"
     gene            38760..40082
                     /locus_tag="PKI64_00995"
     CDS             38760..40082
                     /locus_tag="PKI64_00995"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOD99614.1"
                     /translation="MSKYAPILDAKLTYLYSRTGGSAIKLGLDTISALLRVMKVDPLQ
                     LPCVHVAGTNGKGSVSAMIESVLREAGLHTALYTSPHLIRFNERIRIRGEMIPDDELL
                     RLLDLVEKADQTQAAEAEGRRLGTFFELTTAVAFAWFLAQKVQLAVLETGMGGRLDST
                     NVVTPLLAVLTSVGMEHKSFLGRTLEKIAAEKAGVIKPGRPVVTGRQSPAVMRVIADR
                     AREVGASLILAEERVSIRRTSQDLDGQMLSVETAAGGWAPFRLPLLGDFQLENCALAI
                     AALEWLREELHLPLPPDVIREGLAKTRWPGRCQVVSRDPVFLVDVAHNPGAAQALANF
                     LKSFRGDRPVALICGMLADKDAEGFFKILRPVVDACVLVPLDSERNMPMEKLMAAAKA
                     AKLPAAEGLLPSAVKNAKAWAQKNNGIVVAAGSLYMASAVLYELGYQV"
     gene            40090..40509
                     /locus_tag="PKI64_01000"
     CDS             40090..40509
                     /locus_tag="PKI64_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD99615.1"
                     /translation="MKSHRQELWLNVPARRGYVNLTADVAEAVRASGIREGLCLVNAM
                     HITASVFINDNESGLHRDFERWLEKLAPEKPYTQYDHNRTGEDNGDAHLKRSVMGREV
                     VVAITKGQLDLGPWEQIFYGEFDGLRRKRVLIKIIGD"
     gene            40509..40994
                     /locus_tag="PKI64_01005"
     CDS             40509..40994
                     /locus_tag="PKI64_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD99616.1"
                     /translation="MKNPPPAIEILARGACVRAGKVLVCRNRKHGNLYLPGGHVDWGE
                     DSRHALAREWREELGVDCRVGHFLGVIEQEYQARSGRTCEISLIFAVSCPQLSARQNP
                     PAAESHLEFLWIPLQQLPKSGLLPKIMARLVPQWCAQPAAAAKRWRGFSPAKPAKSPA
                     K"
     gene            complement(40980..41219)
                     /locus_tag="PKI64_01010"
     CDS             complement(40980..41219)
                     /locus_tag="PKI64_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99617.1"
                     /translation="MKYFSLFLIIYEILPIFDLFFVVFSHIFGFSTKFARFLDKNGVW
                     REKNRWWTTLPNFLPGRGVLREMSGGKAARALFRR"
     gene            41305..43059
                     /gene="ilvB"
                     /locus_tag="PKI64_01015"
     CDS             41305..43059
                     /gene="ilvB"
                     /locus_tag="PKI64_01015"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOD99618.1"
                     /translation="MSEKQMIGAEALLQCLEKAGIEIMFGLPGGAAIPLFDALYQLKG
                     KIKFVLTRHEQNAAHEADGYARATGKPAAVLVTSGPGAANTVTGIMTAYMDSVPMIVI
                     TGQSVRGMLGKDAFQEADIFNLTMPIVKHSYLLKSANDIPRVVKEAYHIATTGRPGPV
                     LIDVPKDVGTELCTAALDAEMNLPGYKPEKRGYLPETLDFIADALAKAKRPVIIAGHG
                     AALSRAEKELRELAEKLQIPVTSTLLGKGVFPDSHKLSLEGLGMHGTAYANRAVVECD
                     LMLALGARLDDRILGVPSMFCRNAVKIHVDIDPTEFGKLIKPDFQCQGDIKTVLQDLL
                     PRVKRGDTAEWLKQVEGYKKKWPLTYAKKGGLHPPQVIDALNKLTGGKAIMATDVGQH
                     QMWAWQWYKCDKPWQFITSGGAGTMGFGLPAAIGAQFGRPDELVIAIVGDGGFQMTMS
                     DLATAVIHKLPIKIILMNNHYLGMVRQWQDMFYDERFSQVDLRGNPDFVALAKSYGML
                     ALNLKRGADIPKVLKRALEHPGPALINVEVVMHEEVYPMVPPGKPLEETVLEHPRQKP
                     GRPAGRKNKPAAARAKKN"
     gene            43077..43577
                     /gene="ilvN"
                     /locus_tag="PKI64_01020"
     CDS             43077..43577
                     /gene="ilvN"
                     /locus_tag="PKI64_01020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOD99619.1"
                     /translation="MSTSVPTHNLSIFVANRPGALARIAQTFSRRGFNIESLVVSPAA
                     NSDLSRMTITAKGREAGLEQIIMQCNKLVDVLACREHTDEEVVARELALVKVAADSTR
                     RAEVLQIADHFRAKTVDLADKSIVLEATGSSEKIDAMVDLLRKFKIIELVRTGKVIMA
                     RGERET"
     gene            43865..44362
                     /locus_tag="PKI64_01025"
     CDS             43865..44362
                     /locus_tag="PKI64_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD99620.1"
                     /translation="MKLIRQLHQSKGAGVTDLAKAVGIGGSDASQELRRIQSRGLLRV
                     ERKRSFVFYRFGADPQVPSAAPLVKALLEVLGSSSEKQDAEIIRIAWGLAHPRRGALV
                     QALLMAPKTLAQLGRDTGIPRNSVSRHIGIMEEAGWVAKQGQWLVGTVQPHPIARTLA
                     QLLQE"
     gene            44425..44979
                     /locus_tag="PKI64_01030"
     CDS             44425..44979
                     /locus_tag="PKI64_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOD99621.1"
                     /translation="MQEQRRDIAARLKERRELEEKSAQEVAAALGVSAEVYESYEAGS
                     ADIPASALHEAALFLNADLTELLTGEAPRMKIFCVTRQGGGVKVERRKDYQYRNLAAN
                     FAGRKMNAFEVTVPPDAGEAGTHANSHPGQEFNYVLEGRMRVTIHGNELILETGDCVY
                     FDSNYGHAMQALDGRPVRFLAIII"
     gene            45000..46655
                     /locus_tag="PKI64_01035"
     CDS             45000..46655
                     /locus_tag="PKI64_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD99622.1"
                     /translation="MLKQFVPRSEFASYEDFRAGLAIHTPESFNFAYDVVDHYARTEP
                     TRRAMVWCNDAGDERNVTFADLKDGSDRAAQVFQRHGIRKGDAVLLILKGRLEFWHAL
                     LGLHKLGAIGVPATHMLTEHDLEYRFERAGIRLVLTVPDAALEAAIDQAAAQQAAPPL
                     KMRLHGGREGWFDFAAGMQAADGHWERPSGAAATRNDDTMLIYFSSGTTGQPKMVAHD
                     FTYPLGHIVTAKFWQNVRDGGLHYTVADTGWAKSAWGKLYGQWICGSAVFVHDYDKFS
                     PTKTLEMCAKFGVTTFCAPPTVYRFLIKEDLSKYDFSKLEYAVVAGEPLNPEVYEQFL
                     KATGLRLMEAYGQTEMTVGVGNFVGMEPKPGSMGKPSPLYEMFLARPDGSRCDPGEQG
                     EIVVPQERLRARGMFQGYHRDPEGNARCLRGGVYHTGDVAWQDEDGYFWYVGRDDDLI
                     KSSGYRIGPFEVESALMEHPAVMECAVTGVPDEERGQLVKATIVLAKGYEPSDELQHE
                     LQAHVKKVTAPYKYPRVVEFVRELPKTISGKIRRVEIRAQDGA"
     gene            complement(46685..47131)
                     /locus_tag="PKI64_01040"
     CDS             complement(46685..47131)
                     /locus_tag="PKI64_01040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOD99623.1"
                     /translation="MKKDPVRVYTHRFVFPPEAEDENQHVNNIEYVRLLQESAIAHTR
                     QNGWTPEELHSRDWTWVVRSHFIEYLQPCRAGEPIAVQTWVANFQKIRSLRKYRFVRE
                     SDGTLLATAQTSWVFLNLRTGRPVAIPPEVLAAYQAVPAAEEPGES"
     gene            complement(47128..48603)
                     /locus_tag="PKI64_01045"
     CDS             complement(47128..48603)
                     /locus_tag="PKI64_01045"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOD99624.1"
                     /translation="MNLSTGIALDWFPRAAALAHSSRPLSCGVCEAPPRPPPPFTERH
                     LQCVWADARLRPSLLATAAGEPVQVEHPGEWNTGPGPDFLNAALRVGPDLRRITGDVE
                     IHVRPADWQRHRHADDPRYRRVCAHVSYYPGTLPEAELPPGTLQIALRPALDAQLDFS
                     FDNLDLLSYPVAARATPPPCRAAMLQLTPDQRGAVLDAAGEHRLRRRAEILQAAMLDR
                     GPAQIIYEEVMAALGYRPNKAPFRRLARVLPLELLREKSGGNPWRAYALLLGVASLLP
                     DPTTRRIGDDETKSFLRRCWDAWWPLSGEFLPRRIPRADWVFAGVRPTNRPERRLMAA
                     AQLFAPTPGLPERVADIAKRTQGLDANTLLQLFDLPETPYWANRLSFSAPPVDAPVAL
                     VGLPRARAILVNLLLPAVAALGAPASAWQALLADLPAEEPNELVKQTALRLFGPDHTP
                     RLYRAGLRRQGLIHIHHEYCLNDRSLCATCPLPTSLASTTP"
     gene            48682..48754
                     /locus_tag="PKI64_01050"
     tRNA            48682..48754
                     /locus_tag="PKI64_01050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48716..48718,aa:Arg,seq:tct)
BASE COUNT         9085 a        14364 c        15913 g         9192 t
ORIGIN      
        1 agtcgcgccc cgcgcggggc gcgtggattg aaacctggcg ggggcgggac gggagtcggt
       61 tatctttcgt cgcgccccgc gcggggcgcg tggattgaaa cgtggccatg atgccgctgg
      121 tggtcgttcc gatcgtcgcg ccccgcacgg ggcgcgtggc ttgtgggttg caacgccagc
      181 cggaacttca gcaaggcatt cgaccagcga caaccgacta gcgacattca ggttttcggt
      241 ggctgcgcgg ggcgcgtgga ttgtgggtga caacgccagc cggaaattca gccgagcatt
      301 cgacccgcgt cgaccgggtg gcgacattcg ggttgtcgct ggttgtgggt cggtggtcgc
      361 ggctactttt tggatttttt agttttttgg gcgagctggc gggcgagggt gtcggtggcc
      421 tgcaggatgg attcgtagcc ggtgcagcgg cacaggtggc cgcccaaggc attgagaagg
      481 tcgtcgcgcg tggcgtgggg attgcggcgc atttcgcctt cgagggccat cacaaagccg
      541 ggggtgcaga agccgcattg gacggcatca gcctcgataa aggctttttg gacacgggta
      601 atctcgccgg gaggggccag gccttcaatg gtgacgatgt cagcgccgtc ggcctcaacg
      661 gcgaggacaa ggcaggcgcg gatggcttgg ttgttcatca ggatggtgca ggcaccgcat
      721 tcgccttcgc cgcagccttt cttggcgccg gtgaggtgga gttcctcgcg caagacgcgc
      781 aggagggtgt cggcggcttc gacgtggagg gtgcgttgaa tgccgttgac ggtgagggtg
      841 atgcggtggg tcatggggcg agcctttcga gtagttcgtc caagaagttg cggatggcgc
      901 gttttttctc ggcgggggag gcgcggacgg agccggcgcg cggagcgatt tcgcggatga
      961 tgaggtcgta ggcttggtcc atatcggccg gggtgagggg gcggcggctc aggagggctt
     1021 cggcggcgcg ggggcggtag ggggtgggca tcacggtgcc gaaagaaatc cgcaccacgc
     1081 gcttggcggg aatgcggggc tggaggacca gcgctgcgcc attccagcag gcgaggtcca
     1141 tgccggcggt acgggtgatg cggcgataga agctcaaact gtccgcggct ttgggcagga
     1201 tgatggagag gacgacttca tcgggctcga ggcatgtgcg gttgacgccg gtggcgaagg
     1261 tgtcgaaggg caccaggcgt acaccgcggg tggaggcgat gcggacttga gcttcgtgca
     1321 cggagagcac cacgccgctt tcgcagccgg gggaggagtt gcagatgttg ccgccgaggg
     1381 tggcgcggtg gcggatttcg tggcagccca tgacgtcggc gcattcgcgc agggcggggg
     1441 accaagcagc cacgaggggg gattcgcgca gggcagtgaa ggtttcgcac gcgccgattt
     1501 ccaggccgga tttggtttcg cggatgccgc gcaattccgg gatggctttg atgtccacga
     1561 tgcgttgcac ggttttcttg cggctgcgca tgtcaacgag caggtcggtg ccgccggcca
     1621 tgaggtgggt gcccgcgcgg gtttcggcga gcaggtcttc gagggtctgg ggtttgcaat
     1681 attcgaggtt cataggcgga tggggaggga gtagaggtcc tggccggtgg cgcgggcgat
     1741 ggcgttggcg acgcacgggg cgatggcgac cgtgccgtgc tcggccaggc cgcgcgcgcc
     1801 aaacggcccg ttgcggtcgg gggtttcaac aaagatcacc ttgaagtcgg tgtcgcgcag
     1861 gtcctcgagc gcgggaattt tatagtcggt gaagcgtccg ttgcgcagta cgcccttgtc
     1921 ggagtagatc atggtttcca tgagggtttg gccgagggct tgcaccatgc cgccaaccac
     1981 ctgcccgcgc gcgaggcgcg ggttgatgat gcggccggcg tcaatggccg tgaccagttg
     2041 aagcaccttg atctgcccgg tcttggggtt gacctccagc tcgacggcct gcgcaccgaa
     2101 cgtccacgag gcggagacgt tgccttggcc gatttgcagg ccgggcttgc ggatgccggg
     2161 gatttgcagg tcgggggtgt aggcgccata gcccaccacg gggcggccga tggtacggcc
     2221 atcggcgagc atatagccgt tggtgaagag gccaatgtcg tggtggcgct tgccgatcac
     2281 aacgcggcca tcctgatagc gcactttggc gaccggcacg cgcagggcct tggcggccgt
     2341 ggcgcggatt tgcgcaatgg cgtcttcggc ggcgcgcaca atggcgatgc cgccggtgta
     2401 ggtgcctttg ctgccgatgg tctgccagtc ggcggggaaa agcgcggtgt ccggatcgcg
     2461 cacgacgtgt acgcgctcga ggggcaggcc gagggcttcg gcggcaatct ggccatagac
     2521 ggtggcggag ccctgcccca tgtcgcaggt ggtgatgatg aggttcaccc cgccgtcttc
     2581 atcgaacttg aggatggcgg cgacttgggc gttagtggcc tgcaccggcg atttcatcag
     2641 gctgcatacg gccttggtgc ggaaaccggt gcgggttttg accacgttgc tttgacccat
     2701 cttccagccg acggccttcg cggcctcgcg caggcattgg ggcaaatcgc cctgggtggg
     2761 gtggaggcgc tgggcaatgg cgtttttgtc gccggcgcga taagcatttt tcaaccgcag
     2821 ttccacgggg tccatcttca gctcacgggc gagcaggtcc atcatgcgtt cggccatcca
     2881 ttggccttcg gggtggccgt agccgcggaa ggcgccgacg ggcggcaagt tggtataaac
     2941 cgcgtagctg tccacggcga tgttcggaat gtggtaggcg ccgggcgagt tctggccgcc
     3001 gccgaccgca acgttgatgg cgcattcgcc gtagccgccg ccgctcaaga tatattcgat
     3061 ttgcgcggcg aggacggtgc cgtcgcgctt ggcgccgagc ttgtaacggc ccgtgacgcc
     3121 gcgaccaacg agcgtgccgt tgaacatttc ctcgcgctca tagatcagct tgatccacac
     3181 gcccggcact tggcgggcca cataggcggc gaggggctcg agggtgtagt cgctcttgcc
     3241 accgaagccg ccgcccacca ccggcgcgcg cacgcggacc ttggaaagcg gcaggcggaa
     3301 cgcgcgagaa agatagccgc gcaccacgaa gggggcctgg gtggaggaat agatttccac
     3361 gctgccggcg gggtcccagc gcacaagcgc gccgtgcggc tccagttggc agtggctcat
     3421 gtgcggatag gaaaactcct gttcgagaac cacgtcgcac ttggcaaatg ccttgcgggt
     3481 gtcgcccttg cgcagttgat agtggtggaa aatgttggtg ccgggcttgg gatggaagcc
     3541 cttggcgcat tcgtaggagc cgaggtcggg gtggatcagc ggcgcatcct tggccatggc
     3601 ttggcgggca tcggtcagca cgggcaactc ggcatattcc accttgatca gcgtggtggc
     3661 gtgctcggcc gcggcaaccg tatcggccac caccacggcc accgcctcgc cggcgaagcg
     3721 cacgcggtcc atggccagcg gcaactggtc cacaatgcac gcgccaacca acggcagctt
     3781 gccgccgccc acgtcctgtt cctcccgccg gccggtgacc accgcgcgca cgccgggcgc
     3841 ctgccgcgcc gcgctggtgt caatggatac aatcttggcg tgggcgtatg ggctccgcag
     3901 cacccgccca tgcagcattc ccggaaattg caagtcatcc acgtaaatgg cttgccctgt
     3961 cactttttcc agcgcatcgg cgcggacgac gcttcggcct acctgtttca cgctcgcctc
     4021 ttccttcggt tggttgatcg gaaaacgtag cacatttgcg cccttggaac aaatacaaaa
     4081 aaaccggact tgcggttttg acctgctgaa aatgacgtgg tagcttggcc ggagttgctc
     4141 cgtggcgtcc acgtcggcgg ggcggaccaa catgttcaac ccacacaagg agacggaatg
     4201 aaacccagga agattcgtga aggcgtgcac tggatgggcg cgattgattg gaaccggcgg
     4261 ctatttgact cgttgattcc gttgcccgac ggcaccagct acaatgccta tttggtggaa
     4321 ggcgcggaga aagtcgccct gattgatacg gtggacccgc cgctcctcga tgtcctcaag
     4381 cgccatctcg ccaatgtgcc gcgcgtggat tacgtgattg cccaccacgc cgagcaggat
     4441 cacgccggca gcattcccta tgtgctggag aagtatccca acgccatcgt catttgctcg
     4501 cccaaggcca aagacctgct cctgacccac ctcgatgtgc cggaaaacaa aatcaagacg
     4561 gtcgaagacg gcgaaaccct cgccctcggc ggcaagacct tgacgttcat ccacacgccg
     4621 tgggttcact ggcccgaaac catgagctcc tacctcaagg aggacaagat tctgttctcg
     4681 tgcgacttcc tcggctcgca catcgccacg tcggagctgt atgccggcga tgaccgctac
     4741 gtgatcgaag ccgccaagcg ttactacgcg gaaatcatga tgcccttccg cgccatggtg
     4801 cgtaccaacc tcaagaaaat ccgcgagttg gacttcgatc tggtggcccc cagccacggc
     4861 cccatctacg acaagcctga gatgattctc gctgcctacg aggattggtc ctccgaccgc
     4921 gtcgaaaacc gtgtggtgat tccgtatgtt tcgatgcacg gcagcaccga gctgatggtg
     4981 gaccatctcg tttccgccct cgccgagcgc ggcgtggcgg tgcagaagtt cgagctaacc
     5041 accaccgaca tcggcaagct ggccatggcg ctggtcgaag ccgccaccat cgtggtgggc
     5101 gcgccgaccg tccatgtggg cccccacccg gcggtgttct cggccacgca cttggccaac
     5161 gcgttgcggc ccaagctcaa gtatgccgcc atcatcggct cctacggctg gaatacgcag
     5221 gtcatcgagc gcatcgccgg cctgatcccc aacctgaagg tcgaagtcct cggcaccgtc
     5281 ctcagcaagg gcgtgccgcg cgcggccacc tttgccgagc tcgacgcgct ggccgacacc
     5341 atccgggaca agcatgccac gctctaaacc aaaaattgcg attcagcgca tctacgacac
     5401 ccccgtggat ggctatcgcg ttttggtgga tcggctctgg ccgcgcggcg tctcccgcga
     5461 tgccgcgcgg ctggatgaat ggctccgcga tctggccccc agcgccaagc tgcgccagtg
     5521 gttcaaccat gaacccgccc gttgggaaaa gttccgcgcg caataccgcg cggaactggc
     5581 ccctcagcag gaaacactgg cgcggctgcg cgacatcgcc caacggaaaa aattggtgct
     5641 gctctacgcc gccaagaacg aggactacaa caacgcacgc gtcattcagg aaatcctgaa
     5701 cgaatcatcc tgaaccacgc gggaccgtgc cccgccgtgc acgcgagccc ttcgcggaat
     5761 tcccgctgcc cctcccgcgc cccggtcgcg cagacttggc agaccgcgcc gcgcgcgcta
     5821 tactgccccc tccaaacaag gaaatgccca tgaaaacgat cattattggc ggtgtggccg
     5881 ccggtgccag cacggccgcc cgactgcggc gcatggatga aaaagcggaa attgtcctgc
     5941 tcgaacgcgg cccgcacatt tcctatgcca actgcggctt gccctatcac ctcggcaaaa
     6001 tcatcccgga gcgtagctgg ctgctggtga tgacccccga agacttccgc gcacgcttca
     6061 atgtggatgt gcgcgtgctc cacgaagtca ccgccattga ccgcgcccaa aagaccgtcc
     6121 gcatcctcaa ccgcaaaacc ggtgtcgaaa acgaagaacg ctacgacaaa ttggtcattg
     6181 ccaccggctc ctcgcccatg gcgttgcagc tccccggcat ggacctgccc gaagtgttgc
     6241 cgctgtggac gctcgaagac atggaccgca tcgccaagaa gctcgatgcc ggcgcgaagc
     6301 acgtcattgt ggtcggcggc ggatttgtcg gcatggaact ggccgaaagc ctgcgccacc
     6361 gcggcctcgg cgtgaccttg ctcgaaatgg gtcgccagct tttgccgacc atggacgaag
     6421 aaatgagctc gctgctggcc gaagaactgc gcctcgccgg cattgtggtc gagctcggcg
     6481 cggcggtctc cgcgtttgcc gcggcgcccg gcggcggcgt gacggccacc atcaaggatg
     6541 gccgctcatg gacggccgat ctcgtggcgc tgtgcgtggg cgtgaagccc aactcgcagc
     6601 tcgcttccgc ggccggactg gcagtgggcc ccaaggggca catcattacc gatgctgcca
     6661 tgcgcacggc cgatcccgat attttcgcct tgggcgacgt ggtggaagtc atggaccccg
     6721 ttttcggcac ccccactgcc gtgccgctcg ccggcccggc caatcggcag ggccgcatcg
     6781 tggcggataa cctgctcggt ggctcgcgcc gctacgaggg cacctttggc gcatccgtcg
     6841 tcaaagtggg caaactgacc gccgccagtt gcggccatac cgaagcgcgg ctgaaggcca
     6901 tgggcaagac gtatcagaag gtgtatgtcc acgccggctc gcacgcgggc tattatcccg
     6961 gcgccaccac gctgcacatc aaactgctgt tcggcaacga tggccaaatc tacggcgggc
     7021 aggtcatcgg tgcggaaggc gcggacaagc gtaccgatgt tctcgccact gccatgcgtg
     7081 cgggcatgac cgtgcgccaa ctggcggagt tggagctgtg ctatgccccg ccgttcagct
     7141 cggccaagga ccccatcacc gtcgccggca tgattgccac caacgcgctc gatggcgcca
     7201 cccggctggc ccacgccgat gcgctccccg aaggcgcgct gttgctcgat gtgcgcgaac
     7261 ctgccgaact cccgcagggc gcgctggccg gctccgtcaa cataccgctc cataagctgc
     7321 gttcccgact cttcgagctg cccaaagacc gcctgattgt ggtgtattgc caggtgggcc
     7381 tgcgtggcta tgtggccgaa cgcatcctgc gccagttggg gtatgacgtt gccaacctct
     7441 ccggtggcta tctgacgtgg aaacaattcc agcccggaat gtggatgccc accgatgccc
     7501 aaattgcgct ggccaccacc ggcagcgagg gcggtccccg tctggagttg gaagaagagg
     7561 aacccgccac cgttgtggat gtccgcccgc tgcaatgccc cggccccgtg gtgcgcatca
     7621 agcaggagct cgacaagctg ggcgatggcg ccaccatcaa ggtgctggcc caatccagtt
     7681 ttgcgcctga cctcaccaac tgggccaaca gctctggtca caaggtgtgc aaaatcacgc
     7741 ccacccccac gcatttggaa gctatcctgc gcaagggcgc cgggcccaaa ccagcggtgg
     7801 gcatcgccgc ccctcaatcc ggtgcgatgg ttctgttcag caacgatctc gataaggccc
     7861 tcgccgcgct catcattgct actggcatgg cagccaccgg gatgcaggtc agcatattct
     7921 tcaccttctg gggtctgagc gtcctgcgca aacaccccgg cccgcaggtc aaaaagagct
     7981 tcctgagcac gatgttcggg tggatgctcc cccgtggcgc gcaaaagctg gcgctctcga
     8041 agctccacat ggccggcgcc ggtaccgcgc tgatgaagca tgtcatggcg cagcaaaatg
     8101 tggcctccct gcctgagatg ctgcaccagg cccgcgcgct gggcgtgaag ttcatcgcct
     8161 gcgacatggc catgggcgtc atgggcctca cccgcgagga actcatcgag gtggacgaag
     8221 tcgccggcgt ggccactttc attgcccgcg ccaaggaaag cggcccgacc ctcttcatct
     8281 gaccccaccg accaagccca caccatcggt cggcttactg ggtggcctct ccacggtcaa
     8341 atttccaagg taaaacgttt gatttcactt attgcaataa gtgaaatcta aaagttgtat
     8401 cacgttcaaa tatcacatgt tatttaaaat taataaaaat atagcgaatt gcttgcataa
     8461 atggtaattc ggggtagttt tccatgttct agaaaggagc agaacatgag catgaatatg
     8521 actggattga ttggtttggc ggcgatcggg ctggcggcgg cagcctccgg acagggcgct
     8581 ccggcgttgg tgtttgagcc attgccctac gcctacgacg cgctggaacc ctatattgac
     8641 gcgcagacca tggagattca ctacagcaag caccaccagg cctacttcaa caatctagtg
     8701 aaggcggtgg ctggcacggc catggaaggc aaggcggttg aggcgctttt cccggaaatc
     8761 agcaagttgc cggtggccgt gcgcaataat ggcggtgggc attggaatca taccttcttc
     8821 tggaacagca tggcgcccaa tgccggcggt actccggaag gtgcgctgtt ggccgccatc
     8881 gagaagtcgt tcggctcgtt cgacgagttc aaaaagcagt ttgaggccgc cgcggttggc
     8941 cggtttggca gcggctgggc ttggttggcg gtggatgcca acggcagctt gttcgtgagt
     9001 tcaacgccga atcaggacaa cccgttgatg gatgtggtcg aaaagcgcgg cacgccgatt
     9061 ctcggggtgg atgtgtggga acacgcctac tacctcaagt atcaaaacct gcgcgcgagc
     9121 tacgtgagcg cgttttggaa cgtggtgaac tggccggaag tggccaagcg ctacgcggcg
     9181 actcagcaat aacggacgaa cgattgcttc cccgggcccc ggcaaacaac cggggctctt
     9241 tttttgcacg cagggaatgg ggctacccct tgcgggagaa atcaaggtag aggacgtcgg
     9301 gcgtggtgga ccagccgcgg ggctgttgca ggttggcgag aacccgcaga caaaaggaac
     9361 ggatggcggc ggcatcgagc ggcgcatagg cggaataggt tttggctttt ttgcggcggc
     9421 ggcgtggggt gggggcgaag ccggcggcgt agagtccgcg caggcccagc agccccgcgc
     9481 cggaggattc atcccatgcc ggcgcaagtt gttcgagcag tgcgcgcagg ccgggcagca
     9541 gcgtaatctc cagccagccg gcttgttcct ggcgcagccg gcggaagtcc aacgcccaca
     9601 ggatacggcc ttcggcggtg atggtgcggg cggggcgcac caggcgcgag gcaaaaagct
     9661 gccacacgat cagcggcacg gggcccggcc aaatctcggg gtcaaaaatt ggcacatcgc
     9721 cattgccgag gtgatgggtc tgggcccaga ggcgggcggt atcgttttgg ttgagggtgc
     9781 gcagcgcacg gtagatcagc aagtgcagat aatcgtcggg cagggggagg gtgagctgtt
     9841 ggtcatccaa ataagcggtg acggccgccg cgacggcttc ggcggcggtg gtgtcgagcc
     9901 gggcatcgca caggagcgcg gcgagatgat cgcgcagcag ttcggcctca tcggcagcgg
     9961 tcggcagcgg gatcatggcc tgcccccgcg cgggtgggcg gtgcccacgg cgtcaacttt
    10021 tccacagtgt ggaaaatccc gcccacgcgg gatgaacttt tttgccacag cgtggaaaac
    10081 gcagtgggtt gtgcgggtgg gtcggggcca aacgggggct atttttccac agtgtgaaaa
    10141 ttttttttcc acagtgtgga aaacggagtg agccggcggg agtcgcatgg aaaaaggatt
    10201 attggcgacc aatgagattt tcaatttccg acaaaaattg ggaggcgtcg cggaagcggc
    10261 ggtagaccga ggcatagcgc aggaaggcga cttcgtcgag gcgctccagt ttgtccatga
    10321 ctttgcggcc aatgacggtg gagggcacct cacgttcata ttcggcgctg atttcgtgga
    10381 tgatgtcgtc cacgagcaga tcaatttgcg ggtcggaaat ggggcgcttg acggcggcgt
    10441 ggacaatggc ggcgcggagc ttggcgcgcg aaaattcttc gtggcggcca tcgcgcttga
    10501 gaacagtcag tacggtcttg atgacttcct cataggtggt gaaacggtgg ccgcagtggg
    10561 cgcaaacgcg gcggcggcgc accaccgcgc cattgcggat ggcgcgtgaa tcaatgactt
    10621 tatcgttgtc ctgggcacat ttggggcagc gcatagggac agtgtgcggc ggggcgcggg
    10681 gggaagtcaa gcgcggaggg gcgcgcgaca aacgccgccg cccatgcttg ctttttcccg
    10741 gcggccttgg gaatatgcgc gccatggacg agcgggacga tcaagggatg cggatctact
    10801 tgcgcgaaat cggcaaggtg gaattgctgt cgccagaaga ggaaatcgag ctggcgcgcc
    10861 gcatcaagaa gggcgacgag caggcgcggc agaaaatgat ccgcgccaac ctgcgcttgg
    10921 tggtcaagat tgccggcgat tacgcgcgtc tcggccttcc gcttctcgat ctgatcagcg
    10981 agggcaatat cggcctgatg aaggccgtca cccgcttcga cccgaaaaag ggcggcaagt
    11041 tgagcacgta tgcggcgtgg tggatcaagc agggcatccg ccgcgcgttg gccaaccaga
    11101 gcaaaaccat ccgcctgcca tcccacatgg tggacaagct cttccggatg cgccgtactt
    11161 atgaggccat gacccacgag ctgggccgcc agcctacggc gcaggaagtg gctttgcgga
    11221 tggatgtccc cgtccagcgc gtgaagtatt ggctggaagt gtcgcagcgc gtgacctcgc
    11281 tggatgaacc cattggtgac gagggccgtg aaagccaaag cgacctggtg agcgatccgg
    11341 atgcccgctc gcccttcgat gatttgaacg accgccaatt gctcgacgaa atgctcgaac
    11401 acctgaaagt gttgaacgag cgggagcgca cgattctgga acgccgcttc ggcctgaacg
    11461 gcaaggagcc ggaatcgttg gaagaagtag gcaaaaaact gggcatcacg cgcgagcgcg
    11521 tccgccagtt gcaaaaccag gcgttggcca cgctacggga aatgatgctg gaacactaac
    11581 cggcttcccg tggccggcgg gccccgcgac gagattagga gaacaccatg gcagagcaaa
    11641 acggcaacga aattcaaatg aacgcggcta acttgtaccg cgaggaaaac tatacggatt
    11701 tgcgggccgg tagcctgcgc aagctgattc cggtgaaacc ggatggtacg gatgatccat
    11761 cgcgcccggt catttttacc gccgccaccc aagtgatgac ccccggcggc attttgccgt
    11821 tgagcggcga agtgcccaat gcccagacgc tggccgaggc cgtcgcggga ttcgccccgg
    11881 ccatcaaaca gagcttggcg gatttgcgcg aagaaatggc cgcgctgcag cgcgaacgcg
    11941 cgtcgcagat tgtcgttccg ggccgcgatg ttccgccccc gtccgacttg ctgattcgct
    12001 aaatcctatc cttcagcgct tcgcgcaggt gctccagcgc cgccgccacg gctgcggcgc
    12061 gaatgtcttc ccgcgtgccg ccaaagtggt gctcaaacac gcgcgacaca gccggggtgg
    12121 cgatggcgat aaacaccagc cccaccggtt tttccgcggt accgcccgtc ggcccggcaa
    12181 ttcccgtgat ggcgatggcg caatccgcgc cggtggccgc gcaggcgccc cgcgccatgg
    12241 cggcggcggt ttcggcgctc accgcgccat gcgcggccaa cgtttccggc gtgactccca
    12301 gcaagccggc tttgagtgaa ttggcataag ccaccacgcc ccccacaaac cacgctgaac
    12361 tacctggcag cgccgtcaac tcgccactca ccaagccgcc ggtgcacgac tccgcagtcg
    12421 tcagcaacca gccgcgagcg cgcaacaatt cagcaatctt tgccaccacc ggattcatcc
    12481 ccgccacttt acccaatccg gcccccattt gccaatttta ctggcaaaac aagccgtaaa
    12541 aaatccatgc tcaacgagga ctgagtcctt atccaaaccg gttgcggttt gtgaaaagat
    12601 catagtccca ccgcttgcgt ttgtatccca tttgggttac attccggact gacgttggag
    12661 gttgagatga gtaaaacggc catggtgcgc gccagagtgc agccagaatt gaaaacaagt
    12721 gcggaaaagg tgttccaccg tttggggtta aatgccacgc aagccataac gattttctat
    12781 aaacaggtcg aattacgtga tggcctgcct ttcgaagtgg tgactcccac ctcgacgaca
    12841 aaacaaacgt tggcggattc cgaggctggg cgaaatctgg tggtttgtga gaatgccgac
    12901 gatatgtttc gccagttggg catttgatgt acaccccggt ctttaccaag cagtttgcaa
    12961 aagacatcaa acgctgccag cgccgcggca aagagatgga aaaattcaaa ctgctggccc
    13021 acaccttgct tgcgggtcgg cagcttgatc ctattcatcg cgatcataag ttaacgggga
    13081 gcatgacggg ccggcgcgat tgtcatatcg aatccgattg gattctgatt taccgcattg
    13141 ccgcggacca actcatcttc gagcgcatgg gaacccactc cgacttgttc aaaaaatgac
    13201 taggtcggat caaaacggcc attgaagaca attgaataaa ttcggcagct ccgtcttcat
    13261 caaaagaatt ggttagccat tgtgcttcgt gaaatgggaa gaaaaaatgg ccgctcttgt
    13321 gtttgcctgt gataggatag ggcggtgatg aaaacggtaa tggtcacggg ctcgggcggg
    13381 ttagtggggg cggaaacggc gcggcatttc gcggcgcaag ggtgccgggt gctcgggctg
    13441 gacaacaata tgcgggaata ttttttcggt ccggaggctt cgacgcgcga ggtagtggcg
    13501 gagttgcagg cggcgtatcc ggaggagtac gtgccgttgg cggtggacat ccgggacgag
    13561 gcgacggtgc gccggatttt taccgaagcg aagggcctcg agctggttgt acacgcggcg
    13621 gggcagcctt cgcacgattg ggcggcacgc gagccgttca ccgatttcgg ggtgaatgcc
    13681 accggtacgt tgaacctgtt ggaggccact cggcagtatg cgccggaggc gacgtttgtg
    13741 ttcacgtcca ccaacaaggt gtatggcgac acgccgaacc ggttgccgct ggtggagcag
    13801 gccacgcgct gggagtgcgt ggaaggtccc tacgcggcca acggcattga tgaaaccatg
    13861 agcgttgacc aaacgacgca ttcgcttttt ggcgcttcga aactggcggc cgatgtgatg
    13921 gtgcaggaat atggccgcta cttcggcctg cggacaggag tgttccgcgg gggctgcctg
    13981 acgggaccac ggcaggcggg cgcggagttg cacgggttcc ttgcatattt gatgaagtgc
    14041 gcggtgacag gtcgcgagta ccgcattctg ggccacaagg gcaaacaggt gcgcgatatt
    14101 ctgcattgcg cggatttggt ggccgcgttt gtggcgttcc atcgcgcgcc gcgggcgggc
    14161 gcagtgtaca acatgggggg gggccgcttt agtcattgct cactgttgga ggcggtgacg
    14221 ctgtgtgagg aactgaccgg ccggcacatg aagacgagct atgtggcaac cccgcgcgtt
    14281 ggcgaccata tttggtgggt gagcgatgtc acgcgcttcc aggcggatta tccggaatgg
    14341 caggtgaccc gcaacgtgcg cgacattttg gcggagattc tggagtatca ccgcgcgcgc
    14401 tggactcggg atgggtgaag tcgtggcaga gtccctcaca tttatttttt gcgatggcct
    14461 gctgctggtg cggtcaggca gttggcaacc ggccggggac gacgtgttgg cggaactgct
    14521 gaccacgcgc caactggcgg cggagtatcg ggtgccgccg cagggtcatt gggccaagca
    14581 agtgagcggc gccacggata atgcgccgcc gggttacgaa tgggtgcggt tgcggacgct
    14641 gttgggcgca gatgtgcctt atgccctcgc cgcgtgccgc gcgctgggca tgttgaattg
    14701 gcgcgcgacg catcggtttt gcgggcggtg tggcggtgtg ctggcggaac accacgtgga
    14761 ggtggcgcga caatgcgcgg cgtgcggtca tgtggaatat ccgggaccca cgccggcggt
    14821 gattgtgcgc gtggaaaaag cggggcagat tttgctggcg cggcacgtgc agcgcatccc
    14881 gcatttatgg acgtgtttgg cgggctacgt ggaattaggg gaatcgctgg aggatgccgt
    14941 gcaccgtgaa gtgcgcgagg aagtcggcct ggaggtgggc gacgtgcaat acgtggccag
    15001 ccagcattgg ccatatccga accaactgat ggtgggattt cgtgcgcagt ggcgcgcagg
    15061 cgacttgaag ttgcagccgg aagaactgtc cgaagcgcgc tggtttgacc cagcccacct
    15121 gccggatatt ccacccaagg ggacgatggc ctaccggctg atttgcgaga ccccggcccc
    15181 catgatgggg tagtttaatt tagtcataaa catggctaaa aatgtctttc gggattatcc
    15241 agactttata aactctggat tggttggcaa aaattgccat tcaaatatgg ggagaaaaat
    15301 atacttatta tgttgtgtat taataagtta cattaattat taattccatc cacagtttat
    15361 aaactctgga tggaggctaa attttaccgg aactgcatga aatagttgtc gcgtttttca
    15421 cggccgatgg tagacggctc gccgtggccg ggataaattg ccaggtcctc aggcagggcg
    15481 gcgagtttgc ggagcgattg ggtgagcacg cgggagttac cgccgggcaa atccgtgcgg
    15541 ccgacggaac cgcggaagag tgtatcgccg gtgaagaggg cgttagcggc gggcaggtgc
    15601 caacacacgc cgccgggggt gtgtcccggc gtttccagca cgcgccaggt ggcgccagcc
    15661 agttgaaagg tgtcgtcctc cttcacgaac accagcggca cgtcgggttt ggtgggggcg
    15721 gggtagatgc ccgggagaga attgcgctcg gtgaagcacc agtcggcatc ccgcgcgtgg
    15781 agataaacgg gggcggggtg ggcggcggtt agcgcggcga gcgcggtcac gtgatccaca
    15841 tggccgtggg tcagcaaata gccgatcacg gtgccgccca gctcgcgtag gcgtacgagc
    15901 agttgtgcgg catcgtcgcc gggatcaatc accacggttt cgcgggtggc aggattcgtg
    15961 acgaaataac aattttcttc gataaagcct gtgactcgtg cttcaatgtt catgggatga
    16021 cagcaacaga ttcaagcacg ggcgtcaagg acgcagccga aattgtcttg gcggtgagcg
    16081 gcttgggtgt gggatattat ccgcgggtgg gggaacgctg ggacgtggtg gcggaggtga
    16141 acctgaactt gcgcgcgggg cgcacagtgg cgctggtggg cgaaagcggt tgcggcaaaa
    16201 gcatcacggc ggcggcgctg ggcgggctgt tgccgcgcaa tatgcagcgg gaccgcggcg
    16261 aactccgctg ggcagcgggc gtggatgcgc ggccggggcg ggggctggcc tatgtgtttc
    16321 aggatccggg cgaatgcttg aatccggtgt tcaccatcgg caaccagatt cgggaagtcc
    16381 tgccgccaga agatgtcccc gatgcgtctg ctcggctgac cagcctgttg cgcacggtgg
    16441 gcttcacgga gtccgagcgc gtgctggcga gctaccccca ccaactatcc ggcggaatgc
    16501 agcagcgggc catgctggcc atggcgttgg cggggcggcc acgcgtgctg gtggcggatg
    16561 agccgactac cgcgctggat gtgaccgtgc aggccaagat tctccaactc ctcggccaat
    16621 tgcagcggca ggaaaatctc gcgattttgc tgattacgca caacctcggc gtggtggccg
    16681 aaatggccga tgaagtccac gtgatgtacg ccgggcgcat cgtggaatcc ggcccggtgg
    16741 cgttgttgcg gaatccgcag catccgtatg tgcaaggctt gctgcgagct ttgccgcgac
    16801 tgcacggtga ggcgggccgg ttggtgggca tccccggcac ggtaccgccg ctgggccggc
    16861 gtccagcggg ctgcgggttt catccgcgct gcgcgctggg acgggagatt tgccgcacgc
    16921 aggagccgcc gtggcgggaa aatcaaggcc acggagtggc ctgctggacg cggggcgcgg
    16981 gtagctgggc atgagcacac cgcttctgac ggtggacaat ctgcgcgtga cgtaccgcgt
    17041 ggggcggcgg cgagtggcgg cggtgaaagg ggtatccttt tctgtggcgc gtgggcaatg
    17101 cctgggcatt gtgggcgaaa gtggcagtgg caaaagctca ctggcgcgcg cgattctggc
    17161 gctggagccg gcggccaccg ggcaggtcat ttttgctggc gaagacgtgt tgaagatgtc
    17221 gcgtgccgcg cgccaagtgt ttcggcgccg cgcgcaaatg gtattccagg atccgctcgg
    17281 ctcgttgaac ccgcgactca aaatcggggc agcgctggcg gaagttctgc gcgtgcacgg
    17341 cttggcgcgg aatcgccggg aagccgcggc gcaaacggcc gcgtggctgg ccaccgtggg
    17401 gctggatgcg gagtatgcga cgcgcttccc gcatgagttg agcgggggcc agcgccaacg
    17461 cgtcaatctg gcgcgggcgc tttgcgtggg cgcggagttc ttagtggcgg acgaaccggt
    17521 cagcgcactg gatgtgagcg tgcaggccca aatcctgaac ctgctcaaaa cgctgcaaac
    17581 cgaacgcggc attacgtggg tgctgattgg ccacgatttg gcggtgatgc gccacatggc
    17641 cgatgagctg gtggtgatgc aggccgggga agtggtggag catgggcggg ccgtggatat
    17701 tttggcgagc ccgcaacacc cctacacccg cgaattggtg gccgctgtgc cggatgtgga
    17761 ccgcgcgttg caatgccgcg cgtaagcaca cgggcgcgcc ggtactttgc tgttgaacgg
    17821 gggcaccggg cagggcagta tttgcgcatt ctatgtatgg agtgaaacaa ctctggctgg
    17881 cgcggttgaa aaaaatcgcg ggccccgacg ggcactggct ggccttggcc ggcgtggcgc
    17941 tgctgtggct gacgcccacc aaggggtatg tgccactgat tttgtgggtt gcggggctgt
    18001 atgggctctg gaattggcgc aagctggccg ctgcttggaa caacgtggcg ggctggtgct
    18061 ttgcgctggg gctcgtctgg gcggtggctt cagtggcgtg gagtttttat cccatgggtt
    18121 cggggcgcga tctggtgaaa tcactgccga tggtggccgc gacgatggcg ttgccggcga
    18181 tttttgatcg cccgacgcgc atctggaatg cgctactggt cagtgcaggt gtggtgtcgt
    18241 tgaagttggc gctcgatttg tggcggttgt gggcagcgct gggttggtcc gcgcttttga
    18301 ccgagtcgcg attttatcat ccgtatttgt acaaccatcc gaatgtttcg agcatgatgg
    18361 ccggtctggc cgcgctggtt ttcggcgtgc ggctgttggc gggcggccaa caccgcaagt
    18421 ggcgagcgct gctggtgctg gggctgctgg tggacctgct gtatttggaa gtgctggctt
    18481 cgcgcggccc gcagttggtg tttgcgttgg tggtgctgag cctgccactg gcgttactgc
    18541 cgagtaagcg ggtgcgggtg gtggccgcgc tgttggcggc ggcgattggc gtggggctgt
    18601 ggtgcgcggc ggggtccatc aatccgcgct ttgaagaccg caccatgaag cactacaacg
    18661 accgcgacac catctggggc cattcgtggc tgctggtcac caagcggccg atagtgggct
    18721 acggtttcgg caaaaaagcg tttgagaagg cggtgtacga aaatccggaa ctgcgcgggc
    18781 cgcgggtgcg gtttcattat ccgcatgcgc attcctattg gctgatggtg ccgttccaag
    18841 gcggatttgt ggcgctggcg ctgtggggcg cgggttggct ggcgttgggc ggccggctcg
    18901 cgtaccggat gtgccgctgg acggcacagg cgccaccggc gtggcgcgcc cgggtgcagg
    18961 tgcggctgct gccggcattg ctcggcgcgg gggtgatgtt cattttggtg tatggcatcg
    19021 gcgattatcc cgacaacgcc attcgccatg cgcagtttta tctggcgggg ctgatcatgg
    19081 cgctaacgcg gccggcggtc ccggaggcgg acgcatgaaa atcagcgtcc tcaccacgat
    19141 ctacaatcgg cccgagcatt tgcggctgct ggtggccgca ttgcaggcgc aaacccgccg
    19201 cccggatgaa ctcatcgtgg ccgatgacgg ctccaacgca gagaccgttg cggccatgac
    19261 gcggctgctg cccgcgtgcg gcatcgccgc gcgtgtggtg cggcaggaaa aagacggcta
    19321 tcgcctcgcc gcggcgcgca acatggccat ccgcgccgcc accggcgatt atttggtttt
    19381 tctggattgc gacattgtgc cgctgcccga cacgctggcg gtgcatgagc gcttggcaga
    19441 gtcgcgccgc cttctatgcg ggcatcgcgc actgctggat gaaattgcca ccgaggcgct
    19501 ttttgccgcc atgcccgccc ccgccgaagc ggattgggaa ttggcttggg cggtggcgga
    19561 taaaaccgag ctgcaagaag cggcgcggtt gtttgcgcgg catgccgcgt tgcgccgctg
    19621 gcatttggcg cgggcgcaca agcccaagct gatcgggtgt cacttttcgc tgttccgcga
    19681 cgatgtattc cgcgtgaatg gctttgatga aaacttcgtg gggtggggct acgaagatga
    19741 cgattttgcg cgccggctct acaagtcggg cgtatcgccg ctgggcgtgc tggaagatgc
    19801 acgcgcgctg cacctgtggc atcccacgtt ggcgccctcg ccgctggcac ggcggcgcga
    19861 ccgccccaac cgcgcctatt tccggcgctg gcgggtgcca gcctattgcg ccaacggcct
    19921 gcgcaaaccg gaaacctgat ggcctggcaa gaaccaacgc ggctggtgat tacctgcggg
    19981 cgcggctgtg tgccggcgtt gagcggcgag ctgtgcgcgc tgggccaccg accgcaggaa
    20041 gtctcaccca ccgccgtgga ggtgaccggc aacctgcacg aggccatgcg gttgaatttg
    20101 tggctgcgca ccgctaaccg cgtgttgttc gaggtggccg ggtttgccgt ggagtcgccg
    20161 gatgaactgt atgccgcgac gcgcgacctg ccgtgggaag aatggctgcg cggggatgtg
    20221 cccttccatg tgcacggcgt agcgcagcat gaaaccatcc gcgactcgcg cttcgcgttg
    20281 ctgcgctgca aggacgccat cgccgaccgg tttgtggagc aaacgggcgc gcggccggat
    20341 tcatcggctt cacccgtggg cgcggcgagc atcgcgctct attggaaaga tggcgtggcg
    20401 caactgtttt tggataccag cggcgtgccg ctttcccatc ggggctaccg tcagcaaccg
    20461 tgggtggcgc cgctgcgcga gacgctggcg gcgagcatcc tgctggaaac cggctggggc
    20521 caaaaccccg atgaagcctt cgtggggccc atgtgcggca gcggtacgtt ggcgattgaa
    20581 gcggcgtgga tttgccaaaa ccgcgccccc ggcctctacc gcgaggactt tgcctttttg
    20641 ctgtggaacg gggtggaggc ggatgagtgg cggcagatgc gtgccgctgc ccaacggcaa
    20701 tgccgcaccg cgccccgcgc ttggctggcg gcttctgaca tagcgcccga agccgtggcc
    20761 tgtgcccgcg ccaacgccga gcgggcgggc gtggcccacc tgattcgttt tcaggttggt
    20821 gattttcgcg acgcggccct gccgcagccg cccgcgctaa tcctaatgaa ccccggctac
    20881 ggcgaacggg tgggagagct cgccgagctg taccccctct acgaagccat cggcacgttc
    20941 ttcaaaacca agtgccagga gttgcgcgcc gcagtgttca cgggcagccc ggcgctgggc
    21001 cgcaaaatcg gcctgcatcc tgcccgccgg accacgatgt ggaatggcga tatcgagtgc
    21061 cgcctgctgg aattcgaaat ctacgccggc acccgcgacc cccgcctgct gcgcaagcat
    21121 cgcggcgagt cgagtgcgcc acaatagact atactttgcg cgggggtttc ccttacgatg
    21181 gccgttgtca tgaagtggag cgtgccgatt ctgtgctacc gcaacatctc gccggtgtgt
    21241 ggcatcaccc cggaggaatt tcgtacgcac ctcgaatggt tggccgcgca cggctggcac
    21301 agcatcccgc tggatcaggt cattgactac atcaaaggcg gcgcggaacc gccgcaactg
    21361 tcctacgcgc tgaccttcga cgactgctat ctcgacaact gggtacacgc cgcgccgatt
    21421 ttgcgcgagt atggcgtgaa ggcttctttc gggtgcgtca cggcatattt gcacgacggc
    21481 ccgcggcgcc ccgatgccac cacgcccggc gccgatctgc gcgagctgcc cgtcgggcgc
    21541 gacgcctggg aacgcgcaat cgaacacaac gacccgacgg ccttcatgaa ccgcgccgag
    21601 ttacgcgcgc tggtgagtga agaaggccac agcttgttcg gccacaccca cacgcaccag
    21661 gcgtgcttcc gctccgacaa gccgctggcc gccggcgcag atagcctgca atccggcacg
    21721 cgcggcatct acctcgaaat gcgcgacggc cagccgatgt tccccagcgg ctccgcctat
    21781 gcccacaacg gcttctggcc gacttccaat gatttgctcg atacctcgct cgtggcacgc
    21841 accaccagcg aacgcattga gttttgcctc aacgaattca cgctgtgccg cgaacggctt
    21901 gaagagttgc tggggcagcc gtcgcgcgca atgagctggc catggggcga atatgacagc
    21961 gtggccatcg aggctgcgct gctggcgggc tacgaagtgg cgctatccat ggattgcggt
    22021 gccaaccagc ccggcacgcc gctctttgcc ctgcgccgcg tgaagatgca gggcggtgtt
    22081 ggcgtacggg agctgaaccg gcggttgtgc attgccaaaa atccgctgct ggcgggcctt
    22141 tttcgcaaaa tctaccgcgt cccccgcggt tgacgcggcg cgcggaggag gggggggcta
    22201 ctgttgcagc agatcgttca ggatggcgag gcccttttcc acgtttttgc ggtcggatgc
    22261 ggagagggaa tcgagaatgt tgccaccgtc cgactgacta gtcaggcccg cggccgcatt
    22321 tttcaacagg gtgtccatgt ccggtaccac aaccggcttg cgtagcgggc cgcggatgaa
    22381 tagcggcacc tgcaagccgc catcttcata gggcaaaaaa gcggataaat ctttgccgcc
    22441 ggtaagctgt gcggtttctt cgggcgaggc ccgcaccgtg gcctgcaaat ccagttgatc
    22501 ggcgaacggc aaaatgacgc cgtgcaaatt gagccggtaa tccgcggcag cgaggcgcgc
    22561 gctggatacc gcgatgcgcc cattgcgcag cgccagcttg gccgtggcgt cttcgtagcg
    22621 gttggtggtg acgaggttcg cggtttgttt ggccgctgcc accttggcct tcagcgtggg
    22681 cagcaagcgc agcagcagcg gctggtcaag ctgccgccac atttgatcgg ccagtgagcc
    22741 gccttcgccc acgttgcgca agtcggtgag ttgcaaatcg aggtccgcca ccaacgaagg
    22801 tagcgcttct tcgccaacgg cgtggccgtg cgcggaggcg gtcaactgca ctgtgccatc
    22861 caggcccggc ggagcatatt ccatgttggc ggccaacaaa ttgtgcaaca tcaggccatc
    22921 tgcttgcagg ctggtgagct gataggcgag cgggcaagcc agcaactgca cggcgccgcg
    22981 gatggtcacg cgaccgccat gcgcggcgaa agtgaacgtg gggatggtca gcacgccctc
    23041 gtgcagcagc aggttggcgg cgccgtcgcc cagcgcgttg gccccgagcg tgaggtcggt
    23101 gaaggtcagt tgcgcctgcg cctgcaagtg gcgcaggagc agcgccagta ggggcgactc
    23161 gcccaacgcc accgtgcccg gcggcgcggc gcacggcggc ggattatatt tgaacgcgtc
    23221 tggcaacgcc gcgcccagca gcagatggtc cgccaccggc gcgggcagca gcaggtccgc
    23281 cgtgagtttg gcgtgaatgg ctgacggcat tccgggcgcg gtgatggggg tgtccgcggt
    23341 cacttccacg gcgagccggt cggccagcga gccttgtgcg tgcagcgcaa ccgtcaggcc
    23401 agaaaatgga aactccgcct ccggcaggag ggcttggata tccgcgacat cgccgatctc
    23461 cattcgggcg gtcaggtcca atggccaggc gatgggctgt gcggagtgat cggcgggcgg
    23521 tgggccgctc agtgcaaatt ccagcgtcga atgctcgcca atcgccatgc ggccgctgaa
    23581 gtgcaacgga ttggcagtgg caaagtcggt caactcgcca tccatggcgg aaagcaccag
    23641 cttgtgttct gcgccgttgg tcgcgcgccg gataaactcc acacgggctt ggcgcagatc
    23701 aatgcggcca atcaccaata gcggcgcggc ggcggctgac gttggctccg cgccggtttc
    23761 cgactccgca gccgagcgct ccttggttgt cgccggtttg ccgcggccga tgtccgtgaa
    23821 gttataggag ccatccgcct gctgcacaat cctcaccact ggccggtcca gcgtgacgga
    23881 agtgacttcc ttgcggcgga agtccacctc agcggtgaag gattgaatcg aagcaaagtc
    23941 ctgctggccg tcggcctcct tgagcactgc gttttgcacg cgcagtgcga acgggaacag
    24001 ttggtaatca atgcgcccgt agtcggccgc gcggcccgtg gcgcgcgtga tgaattccgt
    24061 cactgttttt ttgaagtccg gcgtgttcag caaatgccgc aacgtgaagt gcgcggtggc
    24121 caacagaaac accgccaccc cgaccagcca caacaggaag aatccgaatt ttttcatggc
    24181 cggaagccta ccggctatcc gcccaaaagc caaagccgaa gatgccgcat cgccgcaccg
    24241 acaaagagac ttgaagccca ccggagaatc ggggacgtta ccccccatga agccgacacc
    24301 tgaaccgacc gccgtgcccg cacgggccga tattcccgtg gaattgacgt gggacctcac
    24361 gccgctgtat gccacgccgt cagcgtggga agccgacttt gcgcgcctgg acgagttggc
    24421 cgcgccggtg ttggccctgc aaggcaaact ggattccgtg gcggccgtcg cccaactgct
    24481 ggcggcggaa actgcgctcg atcgggtgct cgaacggctg catacctatg cccacctgcg
    24541 ccacgacgag gacaccgcca acaccgataa ccaaacccgc gaagcgcgca tccgcgcgcg
    24601 ttacgccgag ctggccgccc agtgcgcgtg gatcaccccg gagctgctca tgcacccgga
    24661 ggccgacctg cgcgcgtggc gcgacgcgcc cgagctgaag gattaccgcc gcacgctcga
    24721 aaaggttttg cgcaacaagc cgcacgtgct ctccaccgcg gaagaaaccc tgctggccgg
    24781 cgcggcggaa gtgctcgccg cgcccgagca aaccttcagc ctgttgacca acgccgacat
    24841 gacgttcccg gatattcccg gcccggatgg ccccctgccg ctgaccgaag gcacttaccg
    24901 tgcgcacttg gaaaacgctg accgcgcggt gcgccgcgcg gcgttcgaga cgctgctcac
    24961 cggctatggc aaattggaaa acaccgtggc ggccacgttg tcgtcggccg tcaaagcgca
    25021 tgtattcatg gcgcgggcgc gcaagttccc gtcggcgctg gaggctgcgc tcttcgacga
    25081 ccaagtgccg gtggcggtgt acgaaggcct gctggcggca acgcacgaag ccctgcccct
    25141 gttccagcgc tatctcgatt tgcgcaagcg ggcgttgcga ctgccggacc tcgacatgtt
    25201 tgacctctac gcgccgctgg tgccgtcgtg cgccacgcct gtgccaatgg cgcaggcccg
    25261 cgaatggctg ctggccgcct gccggccgct cggggaggaa tatggccgcg tgttggagcg
    25321 cgcctttgcc gaacgctggg tggactggcg cgaaaacaaa ggcaagcgtt cgggcgccta
    25381 ttccagcggc tgctacgata gcccgcccta cctgctgcta aactatcacg accggctgga
    25441 cgacgccttc accctggcgc acgagctggg ccactcgctg cacacctggc tggccaacca
    25501 ggcccagccc ccccgcaccg cgcactaccc gattttcctc gcggaaatcg cttccaccgt
    25561 caacgagttg ctgctgaccc accacttgtt ggcgcaaccc ggcgagccgg cattccgcgc
    25621 acacctgctg aaccaccttt gcgaatcgtt caagggcacg gtgttccgcc agaccatgtt
    25681 tgccgagttc gagcgcgacc tctaccgctg ggaggaggcc ggcacgccgc tcaccgccgg
    25741 gttcctgcgc gaacagtatt acgaattaaa taaaaagtat tactcgccgg cgattgccgc
    25801 ccatgcggct attggctggg agtgggcgcg cattccgcac ttttattatg atttttatgt
    25861 gtacaaatat gccaccagct tctgcgcggc gttggtgttc gcccgacggc tgcgcgccgg
    25921 cgaaggcgtg gcggcctatc tggacctgct gcgggcgggc ggcagcgccg acccgctgga
    25981 cttagtggcg gctgccggcg tggacctgcg cgagccccgc gtcctgcgcg aggcgttcgc
    26041 cgattttggc gcggtcctca ccgaactgga gcaggcgctg gcgctgccgt gaaggcgcgc
    26101 gacgccatcc tgaccgtgct ggacttcgag tccaccggcg ccgtgtccgg ccatgccgat
    26161 gaaccttggc aggtgggcat ggtggagatg cagggcggcc gcgtgacggg acggcattac
    26221 gaaagttttc tgcgcgtggc ggccagccgc ccattcaatc cgtatgcccc ggggcggcat
    26281 gcggtgctgc gccccgtgtt ggccgccgcg ccgacgcccg gcgagctgtg gccacgctgg
    26341 caactgtggc tggtgggccg cccgctggca gcgcacaatg tgggcaccga aaaaaaattc
    26401 gtccagcgcg ccgcccccct gcacgagttc gggccgtggc tggataccct gcaactggcc
    26461 cgccgcgtgc gcccggactt ggccggccat gccctcggcg aggtgtgcca cgcgctgggc
    26521 ctggtggcgc gcttgcagca ggtgtgcccc ggacgcgatt ggcacgacgc gctctacgac
    26581 gccttcgcca gtgcgctgat tctggaatat tgcctcgccc tgcccggctg ggaagccgtc
    26641 agcatcgccg ccctcgccgc cggcccggac gcgaaatcaa gctgttgacc atctgttttt
    26701 attaatttat aggaccgacc ccttcgcctg ttcgggctcg gtgggtcagc gcttgcgccg
    26761 caagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaggac cgaccccttc
    26881 gcctgttcgg gctcggtggg tcagcgcttg cgccgcaagg ttttgctttt cggagacttc
    26941 ttttgggaag ggtggggcgc ggcagaggcc ttatagcggg tgggtttacg cgttgtttcg
    27001 ttcacggggc ggacggggcg gccttctatg tagccgcggt agccgacggg ttcgagctgg
    27061 aagcgcgggg catcggcgtc ggcccattcg tagccgtagc gttcgggttg gtaggtcttc
    27121 atgacgtccc agagcgagaa gatagcttgc tcgaagttct gatcatcgaa gggcggccct
    27181 tggaacacca tcatggtgca ggccgggagg gagatgacct caaagccttc tgggacgggc
    27241 ccggcgtaat cggcggggac ttcgacgccc tgggcgtaga cggaggtatc cgggcgctgg
    27301 agatttttag gcagccagag gcccatgggt tcatggatgg cagccttgat ttggccaagt
    27361 tgagaataga tttcgcagcc gagttcctcg cagtactcga agtagtgcgt ggctttcttc
    27421 ccacgcttga ggatcatttt gcgcgcggga cgggcgacga tctggacgaa tacaacctgg
    27481 gattggggct tttcggtctt ggctttcatg ggggcggttc ctttcgggtt gggcgggtaa
    27541 taactacgca tccggtcggg catgaacagg acaacggacg gttttttctg gcgatactgg
    27601 cgcggtgaaa tcccaaactg acgggtgaac gcgcgcgtga agccttcgtg ggaatcgaag
    27661 acgaagtcga acgcgacatc aatcaccttg cggggcgcgg cgcagagctt gtccgcagcg
    27721 gcggacaggc ggcgctggcg aatgtactcg aagggggcgc gtccggtgta ttccttgaaa
    27781 agccgggcgg catgccacgg ggattagcgg gcggcgcgcg ccagaccgta caaggtgatc
    27841 ggttcggcca ggtgcgcttc gatgaagccc tgcatccgtt tgaccgcctt gatctgttcc
    27901 gagtccattg gggtttcact ccgttgtgcc gccagtacat cacggaaaaa tgaaggattc
    27961 ttgaccgcat ttgcgaaaat agcagtctgc caggacggag aaaagcgatt ggcaggtcat
    28021 ttgtctggtg tgaagccggg cgcggccaaa atgtgcgggc cggttgattt tcgggcgggg
    28081 gtcgggtagg ttcgtccttc ttttatgagc gcgaaacgaa aaattgcagt gttggtgggc
    28141 gatggcatgg gcgacgaacc ggtggcggtg ctgggcggca agacgccgct gcaagtggcc
    28201 aagattccca ccatccgtcg tgtggcgggc ttgggccggg tgcagttggt gcataccgtg
    28261 ccggaaggga tgtcgcccgg aagcgatgtg gccaacatgg gcctgctggg ctataacgcg
    28321 tcagaaaatt ataccggccg cgcagccatc gaagccgccg gcgcgcgctt gccgctggag
    28381 gccgccgacg tggcctaccg caccaacctc gtcaccgtgg aaaacggcaa aatgctcgac
    28441 tattccgccg gcgatatttc cagcgcggaa gcccacgagc tgattgccgc gctgaatgcg
    28501 gctgccgccc gcaccgggct caagttttat ggcggcgtga gttatcgtca tttgctgatc
    28561 tggcgcaacg ggccggccga agtgaccctg atgccgccgc acgaaatttc cggcaagccg
    28621 gtggccgacc atctgccccg cggcccgcgc gccgcggaag tgctggcgtt gatggagctg
    28681 tcgaagccca tttttgcgga gcatccggtc aaccgtgctc gtgtggccaa ggggaagcgc
    28741 ccggccaccc agatttggcc gtgggggttt ggcaaggcca tgagtctgca aaaatatccc
    28801 gagcgttacg gtttgcaggg cggcgtgatt accgccgtgg atttggtgcg cggccttgcc
    28861 gaactgtgcg ggctggaagt aatccccgtg gcgggcgcga ccggttgggt ggacaccaac
    28921 tatgccggca aggcacaggc agcgattgat tgcctgcggc gctccgattt cgtctatgtg
    28981 cacgtggaag cgcccgacga gtgtggccac aagggcatgg ccgaggaaaa ggtgcgcgcc
    29041 attgaaaact ttgacgaaaa agttgtggct ccggtgtggg ccgcattgga agccgccggc
    29101 gagccgtacc ggctgatggt ctgcaccgac caccgcacgc cactgatcaa gcgcgggcat
    29161 acctccgatc cggtgccatt cacctgggtg gatggtccgc tgggcgcggc catgcagccg
    29221 gatgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattg gcggaacggc
    29341 aacgtgggcg aggttctttg gcgcacgacg aagccatcgg gaaacagcag cgcggtttcg
    29401 cggttgtccg ccatttccag cagcaggctg ttggcttctt ccgcggtgat ggccaaggga
    29461 aacgccggta gcggcgcgtt gccggtcacc gcggttttgg tatcgttgta gaggtgacgg
    29521 cgacggggcc gcgccagcaa ccgggtgcgc tttttgccgg cgccgggcgg cggcgtcagg
    29581 aagtagggat gaaagccggc gtagaaggga agggggacga caccggtgtt gtgcacggtc
    29641 agccggatca gcaggccgcg cgtggtggcg gcgtagagcg cttccaacgc gaagggaaac
    29701 ggatattgcg cgcgggtggc gggggtgtcc gtgaggcgca ggcgcagtga gtccagcacc
    29761 ggttcgccga ccacccgcca cggcaagcgc attgcgaagc catggatggg caggtgatgg
    29821 gcttgccctt gtgccagata gcggccgggt gtgccgtcgt gcagtagccg gccgcaaatc
    29881 gggaagagca ggggaatgcc gccgcgggtt tcggtttcct ccgggtccca gaaccacgga
    29941 tgtcggaaca aacattcgcg tccggcgaac ttcagcgagg acaccgtgcc gcccaattct
    30001 ggcacgatgt ccgcgcaaac ccgcgtgtcg ggcgagacaa tcgtcagcac ccggcagccg
    30061 tgttcctcgc gttccaccat catggcgggg agtgaagcac accgcgggag gcttgcgcaa
    30121 gcggcggcgc gcggggtatc gtagccgcat gaacacggac tacggcatgg tgctgaccac
    30181 gttcgcgaat gaaaccgagg cgcaagccct cgcggcggcg ctgctggcgg aacgcctcgc
    30241 cgcctgcgtg cagatgctgc ccatccaaag cgcctaccgc tggcaaggcg ccatccagcg
    30301 cgagccggaa atcctggcgt tgatcaaaac ccgcacggcg ctttatccgg ccgtggaagc
    30361 catgattcgc gcccggcatt cctacgacac cccggaaatt atttttgtgc cgatcgccgc
    30421 tggctccccg ggctatttgc agtggctgga cgagtccacc cgggcggaca gcccgccgtc
    30481 aaaaagcgtg ggctgtcagc cgtcgtagag ggtatcgccg ccgcttcccc cgccgccgcc
    30541 ggggccatca tccagcatgt cgcccatgtc ggcttcgatg gattcagggt cctcgccggc
    30601 ttcgaggcgg cggatcattt cgttcatttc cggcggcatg ggttcgccgg tttcttcggc
    30661 catttttcgc atgaaccggc ccatggcgcg gggatcgttt tcgtcaattc cgtcggcttc
    30721 ggccatcaga cgatccatgc cggggggcat gtcgtcgccg ctaggcgcgg cgggatccgc
    30781 atcggcgttc tccgcgcggc gtttaccgga gtgggcggca aaacgcgaca gggcgcgggt
    30841 cattttgccg tggccgcaac ccgggcaggt ggggggctga tgcgtggcta cgttgcgcac
    30901 aaggaaactg aacaccttgc ggcactgctc acattggtat tcataaatcg gcatgttggg
    30961 cctccaccgg ccttgtgtag caaagcgcgc ggtgggcggc aagtggcgcc gtataatgtt
    31021 ttacaaaaac ccgttaaaat cgcttgttca ggggccgcgg aatcttaaca taatcctaat
    31081 atgctctagt gtgcactttg ccttgacctg tggcgcaggc gtgtgtagtt ggccagatgg
    31141 cagccgcagg caatagtgtg tggagcgcgg cgaaatggag acctgaatga agggcaaatg
    31201 gttgggcttt ttgggtgcgg ccatgtgttg ggtcagcgca gctatcgcgg ccgtgccgct
    31261 accctacttg aacaagcaga ccaatgcggt ggactggacc acgttgccgg ggtggtcggg
    31321 caatgtcacc aattcttcag cggggggctc ggcccgtttc gcggcagcgg gggacacctt
    31381 gacgcttgcc ctcgatggcg cgccgaccac gctgacgttc acgctgcgcg gctactcaaa
    31441 tgcggtgggc gtggcgccgg ccagctttat ggttgaagaa tccggcgatg gcaacgtctg
    31501 gaactccacg ccggtggcgg atattggcga agattcgctt tcggtagccg ccgcggcgtt
    31561 tggcccgtat gaattgctca gttccacgcg ttacctgcgc ttcacctatg tggaccacta
    31621 cgcgtttgac atcgggctga acaatgtggc ggtggccggc ggaccggccg cgccgcgcgt
    31681 ggtgtttgtg gaccggccgg agggattcat cgtggcgcag ggcgccgaga atgaagtcat
    31741 tcaagcggtg gtggtaaatg gcgggggcta ttggttcggc tccgaggcgc agggcaaaga
    31801 gccgtgggag agtgataacg gcggcacagc cttgagtgaa tcgccctacg acaagtatta
    31861 tatcaatacg gcggccaccg gcaccttttt tgccacggcc aacggccgtt gtgacaacac
    31921 ggatgaggtg gtgcccggca cgattcattt taccgtggcg ccggggtatg ccgtggactt
    31981 gcaagttggc gatcatggca cggctaccgt ggcggtgtat ggccgggcag ccaccaatgc
    32041 cccgcggggc gcactggtga ccatcctgcc cgtgccggac gccggctacg ccaccggcgt
    32101 ccttcaactg aacggcgcgc cgattgcggg taccaccttc atcatgccgg acgggcccgc
    32161 cgtggtaagc gtgtcctttc gtaaaaaagt cgccggcgat ccaacgctca tcatctccca
    32221 gtattacgag ggagcgggcg aaaacaaatg gatcgaactg tacaatgccg gcgcggagcc
    32281 agtggatttg ggcgcggcgg gctaccggct gggcgtgtgg cagaatgcca aacgcgaaaa
    32341 ctggaaaacc aacgcacccc cggccgtggc cacggtgctg acgggtatca tcgccccggg
    32401 cgaaacctat ctggtatgcc acgtcggcac caaggctccg gcgtatgctg tcgcgaacgt
    32461 ggtttccaac ggcatgctgt tcaacggtga tgacagcgtg gtgctctaca ccgggacgga
    32521 atacaacctg gccaacgtgg tggatgcctt cggcatggtg gggagcaact cggtcaattg
    32581 tagttttgtg cgcgcggtgg atgtggtgtc tggagtcaac acggatttcc agtccaacca
    32641 atgggtgcgg tttgaatatg ccgcggtgga caccgcggag gtgcaagtgc cggagcggct
    32701 gggatggcac actacggcca cgcctcaacc cacagaaccg cccaagctgg acaacctaat
    32761 tagtacggag ggtggcttgg tctttgaaat ccccaccaac tacaccgact acaccattta
    32821 tggcgcggcg gaattgcaat ccggtgcgcc ggcgtgggcg ggttccaata ttggcgaccg
    32881 ctgcagcgtg gaattggcca gcaatctctg gcgaatcact gtgcccacca ccctcggtca
    32941 gcggcaaatg atttggctct ccatccccgg aggcaactaa atgactacgc gcgggacacg
    33001 acggccgggc gggggcttgc gcgtggggct ggcgctgttg gccttcctgc tcgcggcacc
    33061 gctggcccag gcccaatatg gtggcgctgg cagctttgtt aaaatcacgt cccgggcgga
    33121 cgtggcggat ggttattacg ttgtggccaa cagcgatgga acaatcgcca tgtcgggcac
    33181 caatcccgga gcgtattttg agcggtctac cgttagcccg gtcaatgaca ccttgactga
    33241 tccgccggca gtcaacgtgt ggctgctgca aacgaatagc aattatggcg ggttcaccat
    33301 cttcaatgaa accagcaaca aatatgtgtc ttttgccgga accgccaacg ccgcgtatgc
    33361 cgctggtgca gtgaatggca cgactggcgt gtggtctttt gcctggcgca gcgaccttgg
    33421 ttgcttcgcg gtcagcaatg tggcacggcc ctaccgctat cttcaataca acaccggttc
    33481 gccccggttt tcttgctaca gcaacaacgc cagcatgcaa aatttgacgc tgtacaaaat
    33541 gttggcggcc acgcccactg cgccggtatt caccagcggc agcggcccgt tcaacgccac
    33601 ggcgttgagc gccaccaact tcgtggtgac ggccagcggc cagcccgcgc ccacgctggc
    33661 gctgcaaggt cagactgcgt cgggaggttt cagcttcacc gctgcaactg gcgtgttgaa
    33721 ctacaccccg cctgtcgccg atatcggcac gcaaaccttc acgttcacgg ccagcaacga
    33781 ggtcggtgtg gccacgcaaa ccatgacggt cgtcgtgtcc gcggccccgg tttacgtgcc
    33841 ggaggtgtcg gtgaccaacg tggccacaaa cggcttcacc gtccaatgga cagcgtgcac
    33901 cggcgccact ggctatcagg tgcaggtggc cacggatatg aattttacgc aggccagcag
    33961 cggcgaaacc accaatgtgc tgaccaatgc tgcaggctca gcctccccgc cgaccgggtg
    34021 gacgtataac atcagcagca agagcagtag ctatttgatt ttgttggccg acacccatga
    34081 ggtgcgcagt cccatttttt ccacggaggg tttgaccgcg ctttccgtag attttctcgc
    34141 ccgcacgtat ggcggagtac agagtggtgt caatgacgtg gaagtgttca tttccactga
    34201 tggcggcacc aactgggtca gtttgggcac tgtcaggccc acggatagca acttgaggca
    34261 gtattcggtg gcagcttcgg cctacgtggg ttatgcgcag gtgtgcttgc gctggaccac
    34321 acccaacgcc acgggcgcgg ttggtgcggg aatcaaggcg ttgaatgtta ccgggtcgga
    34381 aacgggtgcg tttggctcgc tggtggtgga tgttactacc agcgcactcg ccaccaatat
    34441 cactggcctc gcgttggaca ccgcctatta cgttcgggtg cgccggttgc cggtggagga
    34501 atggtcggcg gtagtgccgg tcaccaccgc gggcgcggtg cccactgcgc catggtttat
    34561 cagcagtgcc gggccatatt cggtggcagc cggcgacatg gtggacttca tcgtcagcgc
    34621 gttgggtgtt ccgacgcctg tcctggccct cagcgcggcc acagcgcccg gcattgatta
    34681 tgaatttgat cccaacgacg gctacttcgt gtatcaaccg ccgttggcag ccagtggcag
    34741 cacgcaagtc ttcacgttca ctgccagcaa tacgcaggga gtggccaccc aaacggtgac
    34801 cgtgaatgtg gcgacggtga cggcgccggc gtttggcgcc aatcctggac cgctggtcgc
    34861 cacggttggc gtggcctgcg tgttcacggt tagcgccaca ggcttgccgc cgcccgcgct
    34921 ggcgttgcag tcccaaaccg caggcagcgg atacaacttc acgccggcca ccggcgagtt
    34981 gagctacacc ccacccgccg gcgatatcgg ctcgcgttcg ttcaccttca ccgctggcaa
    35041 cgtggccggc acggccacgc aagtggttca agtgacggtt cacgccttgc caaccattga
    35101 tcccttgccg accatttcca tcgctgctgg tcaaaccacc aatgtgtgga ttgtcgcgcg
    35161 cgaagcggag ggcgattcca tgacgcttgc cgccagtaat ttgccggcga atgccgcgtt
    35221 ctccaccgtg tccggtacca gtgtcatcag caacctgttc acttttgcgc cggactccaa
    35281 ccaaatgggg caggcgtaca ctgtggcctt cactgccagt gatccacacg gttccgtcat
    35341 ccagacgttg agcatcactg tggccagcgc cgatccttgg gcggattatt atgcctcctg
    35401 ctacgaaaat ggcgtgctca aaaccggcaa tgcgttgaag tccgcgctgc acgacatcat
    35461 cagcccccat accagttaca gttacgacgc agcgattacc attctgagcg aaattgacga
    35521 atgtcccacc aacaacacca tggtgcagtt actttatttg cagcacggcc gtgccaagag
    35581 cagtttcggc ggaagtaacg gccaatggaa tcgcgagcac gtttgggcca attcccacgg
    35641 cgttaacgat aaattgccgg cctattcgga cgtacaccat ttgcacccca cggatgtgca
    35701 ggtcaacagc acccgcggca acaaggattt tgacaccgtg agtgggtcgg tcagcaacta
    35761 ttcctataat agcacttcat ttgagccgcc caatgccgcc aagggggatg tcgcccgcgc
    35821 gttgttctat atggcggtgc gctatgacgg caccggtggc gtgagcgact tggaactggt
    35881 ggatagcgtc ggcacgtccg gcgccaagca tggcaagctt tccacgttgc tgaattggaa
    35941 tgagcttgat cccgttaacg actacgaaat ccggcgcaac aatttggttt attccaactg
    36001 gcagggcaac cggaacccgt ttgtggatca cccggagtgg gctcgcgtgg tgtttgatcc
    36061 caactatgtt ccgttggctt cgctgacttc gtttgccgcc acgcccaatg gttccagcca
    36121 gattgatctt tccttcgcct acaccggctc aggcgatggc gtggtaattg tgtggaatga
    36181 aaccggctcg ttcagcgcgc cctccggcac tgcgccagcg gtggggcagt catttgcggg
    36241 cggcacgttg ctgtacaagg gcgccgtctc gccgcaaagc cacaccggcc gcaccgcttg
    36301 ccagaaggtg tattacaagt gctggactta cacgggcaca aattattccg cggtgggcct
    36361 gacggcggat gccacaaccg ccggcccggc ggctcccgcc agcgcgtggg ccagtgccac
    36421 caataccacc gacttcaccg cggcgtggag cgcggtgtca ggtatcagcc aataccttgt
    36481 ggacgtggtg acgggcacga catttggcgg cgcaggatcc gtttgggtgc cgggttactc
    36541 gaaccgcgcc gtcagcagcg ccaccagctt gtccgtcacg ggattgactg ccggtgccac
    36601 ctactatttc cgcgtggccc ccgtgcccaa ctgcgatacg cgtttctcgc ccactggcac
    36661 ggtcaccacg ctcggtgaaa ccctcacccc gttccagcag tggctccaaa acacccgcgg
    36721 gcagaacatc aacgatccgc acttctcgcc ggaggccgat tatgacggcg atggcatgac
    36781 cacgtgggag gaatatctgg ccgatactga tcccgccagc gccgcgagcg tgctggcact
    36841 gaatggttgg ttctccgtta gcgatcaccg tatacgcttc tcgttcccgg ccagcaccgg
    36901 ccgcttctac cgcttggagt attgcaccaa cctgacgaac accactggcg tggcctatag
    36961 caatcttggc tggggcacac ccggcatggt catcaccaac ccgggaacca gcaacgcctg
    37021 gttctggatt ctgcgcgcca gccccaacgc gccgtagcgc agcgaaaatc cgatggacgc
    37081 cgccgggtgg gtggggcaca ctccgcccat gcaatggccg ttgcttgata tgctcttttc
    37141 ccgcacgtgt gagggctgcg gcaacgatct ggccacggag ccgggcgcgt tgtgctggga
    37201 ttgccgggtt ggcctcaagc ccgtgcaagt gcccttctgc gaacgatgcg gcgacccggt
    37261 ggcaggagtg accggcggcc cgttcgagtg tacctggtgc cggcaaaacc aaccggcctt
    37321 cgtttgggcg cggtccgccg tgcgctatga cgggattgct gctgcctgta tccggcgctt
    37381 caagtatcat gccggtgtct ggttgcaggc ggagctggtg gactggctct gggcgctctg
    37441 gcgcacctgc ccggaaccca aggtagctga tttggtcgcc cccgtgccgc tgtaccgccg
    37501 ccgcgaacgc gaacggggct tcaaccaggc tgctctgttg gggcgcagcc tggccaaacg
    37561 gatggggatt cccttccgcg gcaacctgtt gcgtcgtcat aagcccaccg caacccaaac
    37621 tcatttgaca gccgcccagc gggttcataa tgtacgaggc gttttctccg ccccggcgcc
    37681 ccgtcggctg caaggggcgc gcgtggtgct ggtggatgat gtcatgacca ccggtgccac
    37741 cgtaaacgag tgtgcgcgtg ttctaatggc cgccggcgcg caggcggtga tgattttgac
    37801 ggtggcccgg ggttgacatc gccacgggcc gtgcaacaat ggatgttcaa gcatgacctt
    37861 tttcggtaag aaaaaaaccg tggtcaagcg ggcgcgcaaa cgcgacatgc cggatggctt
    37921 gtggctgaag tgccccgcct gcaacgaaat gatttttggc gaagagcagg aacaagctct
    37981 gcgcatgtgc cccctgtgcc accaccactt ccccatgagc cgcaacgaac gtctggccat
    38041 gctggcggat cccggttcct tcgtggagtg ggacaaggat ctctacagca ttgattttct
    38101 cggctttgtc ggtgtggcgt cctatcagga aaaattggaa tccaaccgcc gtaaaaccgg
    38161 cgaggtggat gccgtcacgt gcggccgcgc acgcctcaac ggccgggcca tcgcactggg
    38221 ggtgatggat tttgaatttc tcggcgccag catggggtcc gtggtcggcg aaaaaatcac
    38281 gcgcttgatc gaacgcgcca ccgcggaaaa attgccggtg atcgtcatct gcgcctccgg
    38341 cggcgcgcgg atgtatgaag gcatgatgag cctgatgcaa atggccaaga ccagtggcgc
    38401 gctggcccgc catgccgagg ccggcctgcc gtacattccc gtgttgaccc accccaccac
    38461 cgccggcgtc atggccagct tcgccacgct gggcgatgtg atcatcgccg agcccggcgc
    38521 gctgatcggc tttgccggtg cgcgcgtcat caaggaaacc atccagcaag atttgccgcc
    38581 cggcttccag cggcccgact tcctcctcaa acacggcttg ctcgaccaga tcgttccccg
    38641 caatgagctc aaggccacgt tggcccgcct gctcgatgcg ttctgtgggg tgttgcagcc
    38701 gtcggaaccg ccggaaatcc cggcctgagc cgggtcggcc cgccttttta ttggtcatta
    38761 tgagtaaata cgcccccatt ctggacgcca aactcacgta cttgtacagc cgcaccggcg
    38821 gcagcgccat caagcttggt ttggatacca tctccgcgtt gctgcgcgtg atgaaagtgg
    38881 acccgttgca actgccgtgc gtgcatgtgg ccggcaccaa cggcaaaggc tcggtttccg
    38941 ccatgattga atccgtgttg cgtgaggcgg gcctgcatac cgcgctctac accagcccgc
    39001 acctcatccg gttcaacgaa cgcatccgca tccgcggcga gatgattccc gacgacgagc
    39061 tgctgcgcct gctcgatctt gtcgaaaagg ctgatcaaac ccaggccgcc gaagccgaag
    39121 gccgccgcct cggtactttc ttcgaactga ccaccgccgt ggcgttcgcc tggttcctcg
    39181 cccaaaaagt gcaactggcc gtgctggaaa ccggtatggg cgggcggctc gatagcacca
    39241 acgtggtgac gccgctgctg gcggtgctga ccagcgtggg catggagcac aaatcgtttc
    39301 ttggccgcac gctcgaaaaa atcgccgcgg aaaaagcggg ggtcatcaaa cccggccggc
    39361 cggtggtgac gggccggcaa tcgccggcgg tcatgcgagt gattgccgac cgcgcgcgcg
    39421 aagtgggcgc ctcgctaatt ctcgccgaag aacgcgtcag tatccgccgc accagccagg
    39481 acctcgacgg ccaaatgctc tccgtggaaa ccgccgccgg cggatgggcc ccgttccgct
    39541 tgccgttgct gggcgatttt cagttggaaa actgcgcgct cgcgattgca gcgctggaat
    39601 ggctgcgcga ggaactgcat ttgccactgc cgccagacgt catccgcgaa ggcctcgcca
    39661 aaacccgctg gcccggacgc tgccaagtgg tttcacgtga tccggtcttc ctcgtggatg
    39721 tggcgcacaa tcccggcgcg gcccaagcct tggccaattt cctcaagtcg ttccgcggcg
    39781 atcgccccgt agcgctgatc tgcggaatgc tggccgacaa ggatgccgaa ggctttttca
    39841 agattttgcg ccccgtggtg gatgcctgcg tgctggtgcc gctcgatagc gaacgcaaca
    39901 tgccgatgga aaaactgatg gctgccgcca aagccgccaa gctgcctgcc gccgaaggcc
    39961 tgctgccctc cgctgtcaaa aacgcgaagg cttgggcgca aaaaaataac ggcatcgtgg
    40021 tggccgccgg ctctctttac atggcctcgg ccgtgcttta tgaattgggt tatcaagtgt
    40081 gaggcgccca tgaaaagtca tcgtcaggaa ttgtggctca acgtccccgc gcgccgcggc
    40141 tacgtcaatc tgacggccga tgtggccgag gccgtccgtg ccagcggcat ccgcgagggc
    40201 ctgtgtctcg tcaatgccat gcacatcacc gccagcgtgt tcatcaacga taacgaaagc
    40261 ggcctgcacc gcgactttga acgctggctc gaaaaactcg cgcccgaaaa accctacacc
    40321 caatacgatc acaaccgcac cggcgaggac aacggcgatg cccacctcaa acgctccgtc
    40381 atggggcgcg aggtggtcgt ggccatcacc aagggccagt tggacctcgg cccgtgggaa
    40441 cagattttct acggcgagtt cgatggcctt cgccgcaagc gtgtgctgat caaaatcatc
    40501 ggcgactaat gaaaaaccca ccccccgcca ttgagattct cgcccgcggc gcttgcgtgc
    40561 gcgccggcaa ggtgctcgtg tgccgcaacc gcaaacacgg taacctttat ttacccggcg
    40621 gccacgtgga ttggggcgag gactcccgcc acgccctcgc gcgcgaatgg cgcgaagagt
    40681 tgggcgtaga ctgccgcgtg gggcattttc tgggcgtaat cgagcaggaa tatcaagccc
    40741 gcagcggccg cacctgtgaa atctccctga tttttgctgt gagttgtccg cagctctccg
    40801 ccagacaaaa tccgcctgct gccgaatctc atttggagtt cctctggatt ccgctgcagc
    40861 agttgccgaa atccggcctg ctgcctaaga tcatggcgcg tttggtcccg cagtggtgcg
    40921 cgcaaccagc cgcggccgcc aagcgctggc gcgggttctc tcccgccaag ccggccaaat
    40981 caccggcgaa ataacgcccg cgcggctttc ccgccgctca tctctcgcaa tactccgcgc
    41041 cccggcaaaa aattgggaag ggtggtccac cacctatttt tttctcgcca caccccattt
    41101 ttatccaaaa atcgtgcaaa tttggtagaa aaaccaaaaa tatggctaaa aacgacgaaa
    41161 aataagtcaa aaatcggtag tatttcataa ataatcaaaa ataatgaaaa atatttcata
    41221 aattgcgatt atttttcttg cgccgccaaa aatatgagat attttgatgt cgaaatttga
    41281 tgattttcaa attggaggca aaaaatgagt gaaaaacaga tgattggcgc ggaagcgctc
    41341 ctgcaatgcc tggagaaggc cggaatcgaa attatgttcg gtctgccagg cggcgcggcc
    41401 attccgctgt ttgatgcgtt gtatcaactg aagggcaaga tcaagttcgt gctgacccgc
    41461 cacgagcaaa acgcggcgca cgaagccgat ggctatgcgc gcgccaccgg caaacctgcc
    41521 gcggtactgg ttaccagcgg ccccggcgcg gcgaacaccg tcaccggcat catgactgcc
    41581 tacatggatt ccgtgccgat gatcgtcatc accggccaaa gcgtgcgcgg catgcttggc
    41641 aaggatgcct tccaagaggc cgatattttc aacctcacca tgccgattgt gaagcatagc
    41701 tatctgttga agagcgccaa cgatattccg cgcgtcgtca aggaggctta ccacatcgcc
    41761 accaccggcc gcccggggcc cgtgctgatt gacgtgccca aggatgtcgg caccgagctg
    41821 tgcacggctg cgctcgatgc cgaaatgaat ctgcccggct acaagcccga aaaacgcggc
    41881 tatttgccgg aaacgcttga tttcatcgcc gatgccctcg ccaaggccaa gcgcccggtc
    41941 attattgccg gccacggcgc cgcgctgtcc cgcgcggaaa aagaactgcg cgaacttgcc
    42001 gaaaaactcc agattccagt cacctccacc ctgctgggca aaggcgtctt ccccgattcc
    42061 cacaagctca gcctcgaagg actcggcatg cacggcaccg cctatgccaa ccgcgccgtg
    42121 gtcgagtgcg acctcatgct cgccctcggc gcccgcctcg atgaccgcat tctcggcgtg
    42181 cccagcatgt tctgccggaa cgcagtcaaa atccacgtgg atattgaccc caccgagttc
    42241 ggcaaactga tcaagcccga tttccaatgc caaggcgaca tcaagaccgt gttgcaggac
    42301 ctgttgccgc gcgtgaaacg gggcgatact gccgagtggc tcaagcaagt cgaaggctac
    42361 aagaaaaaat ggccgctcac ctacgccaaa aaaggcggcc tgcacccgcc gcaagtcatt
    42421 gatgccctca acaagctcac cggcggcaag gccatcatgg ccactgatgt cggccagcac
    42481 cagatgtggg cttggcagtg gtacaagtgc gacaaaccct ggcagttcat cacctccggc
    42541 ggcgccggca ccatgggctt cggcctgcct gccgccatcg gcgcccagtt tgggcggccc
    42601 gatgagctgg tcattgccat tgtcggcgat ggcggcttcc agatgaccat gtcggacctc
    42661 gccaccgccg tgattcacaa gctgcccatc aagatcatcc tgatgaacaa ccactatctc
    42721 ggcatggtcc ggcagtggca ggatatgttc tatgatgagc gcttcagcca agtggacctg
    42781 cgcggcaatc ccgacttcgt cgctttggcc aagtcctacg gcatgctggc cctcaacctc
    42841 aagcgtggcg ctgatatccc caaagtgctc aaacgcgcct tggagcatcc cggccctgcg
    42901 ctcatcaacg tggaagtcgt catgcacgaa gaagtgtacc ccatggttcc gcccggcaag
    42961 ccgctggaag aaaccgtgtt ggagcatccc cgccaaaaac ccggccggcc cgctggacgc
    43021 aaaaacaaac ctgccgcggc ccgcgccaag aaaaactgaa accagggagt tgaaacatga
    43081 gcacctcagt ccccacccat aacctgagca tttttgtggc caaccgcccc ggcgcgttgg
    43141 cgcgcatcgc gcagaccttt tcgcggcgcg gcttcaacat cgagagcctg gtggtctccc
    43201 cggccgccaa cagcgacctc tcccgcatga ccattacggc caaaggtcgc gaagccggcc
    43261 tcgagcagat catcatgcaa tgcaacaagc tcgtggatgt cctcgcttgc cgcgaacaca
    43321 ccgatgaaga agtggttgcc cgcgagctcg ccctcgtgaa ggtggccgcc gacagcaccc
    43381 gccgcgccga agtcctgcaa attgccgatc acttccgcgc caagaccgtg gatcttgccg
    43441 acaagtccat tgtcttggaa gccaccggca gctccgaaaa aattgacgcc atggtggacc
    43501 tcctgcgcaa gttcaagatc attgaactcg tccgcaccgg taaggtcatc atggcccgcg
    43561 gcgaacgcga aacctgatct cgcccgcctc ataaacccgg ttccgctcgg ctcaaatggc
    43621 ggaggggtgc caagaggtat ttcgagcaac tgtccagagt ttataaactg tggacagttt
    43681 tcttatatta cgcatttaat tctatatgag ttaaatacac atattttatt gctgtttttc
    43741 aaaaccacga attgactgca ctgtccagag tttataaagt gtgggcatgg ttaagctaaa
    43801 gaaaatacgc caattgaacc caacgttgtg gcggacgtgc cgggtgcttg ccggagaaac
    43861 gcgcttgaag ttgattcggc agttgcatca aagcaagggg gccggtgtta cggacttggc
    43921 gaaagcggtg gggattggcg gttcggacgc gagtcaggag ttgcgccgga tccagtcgcg
    43981 ggggctgctg cgggtggagc ggaagagatc gtttgttttt tatcgttttg gcgctgaccc
    44041 gcaagtgccc tcggctgcgc ctttggtgaa ggctttgctg gaggtgttag gctcttcttc
    44101 tgaaaagcaa gatgccgaaa ttatccggat tgcctggggg ttggctcacc cgcgacgtgg
    44161 tgcgttggtc caggcgctgc tgatggctcc caaaacgctt gcccaactag gccgcgacac
    44221 cgggattccg cgtaatagcg tatcgcggca tatcggcatt atggaggaag cggggtgggt
    44281 tgcgaagcag ggccaatggc tggttgggac ggtacaaccg catccgattg ctcgcacttt
    44341 ggcgcaactt ctgcaagagt aggggggctg gtggcaggcg ggccaaggac cgactgacca
    44401 ggccacaaca aaaggagtcg tgccatgcag gagcaaagaa gggacatcgc cgcacggttg
    44461 aaagaacggc gtgagttgga ggaaaagtcg gctcaggaag tggcggcggc gttgggggtg
    44521 tcggcggagg tgtacgagtc ctacgaggcg gggtcggcag atattcccgc cagcgcattg
    44581 catgaagcgg ctctttttct gaatgcggat ttaacggaat tgctgacggg cgaagcgccg
    44641 cgcatgaaga ttttttgtgt cacgcgccaa ggaggcgggg tgaaggtgga gcgccggaag
    44701 gattaccaat atcggaatct ggcagccaac tttgcggggc gcaaaatgaa cgcgtttgag
    44761 gtgacggtgc cgcccgatgc cggtgaagcc ggcacacacg caaactcgca tccggggcag
    44821 gagttcaatt acgttctcga gggacggatg cgtgtgacca ttcatggcaa cgagctgatt
    44881 ttggaaacgg gggattgtgt ctacttcgac tcgaactatg gccatgccat gcaagcactg
    44941 gatggccgcc cagtccgttt tctggccatc atcatctaac ccggaagctt tcagtgagcc
    45001 tgctcaaaca atttgttccc cgcagtgaat ttgcgtccta tgaagatttc cgcgcggggt
    45061 tggccatcca cacgccggaa tcattcaact ttgcctatga cgtcgtggac cattacgccc
    45121 gcacggagcc cactcgccgg gccatggtgt ggtgcaacga tgcgggcgac gaaaggaatg
    45181 tcacgtttgc ggacttgaaa gacggcagcg atcgcgcagc gcaggttttt cagcgtcatg
    45241 ggattcgcaa aggcgatgcg gtgctgctga ttttgaaggg gcggctggag ttttggcacg
    45301 cgctgctggg gctgcataag ttgggcgcga ttggcgtgcc ggccacgcac atgctgacgg
    45361 aacacgattt ggaataccgt tttgagcggg cgggcatccg gctggtgctg acggtccccg
    45421 atgccgcctt ggaggccgcg attgatcaag ccgccgccca gcaagcggcg ccgccgctca
    45481 aaatgcggct gcatggcggg cgggaggggt ggttcgattt tgccgcggga atgcaggcgg
    45541 cggatggaca ttgggagcgg ccaagcggcg cagcggccac acgcaatgac gacaccatgc
    45601 tgatctattt ttcgtcgggc accaccgggc agccgaagat ggtggcgcac gattttacgt
    45661 atccgctggg ccacattgtg acggccaagt tctggcagaa cgtgcgcgat ggcggactgc
    45721 actacacggt ggcggatacc ggttgggcga agtcggcttg gggcaaactc tacggccagt
    45781 ggatctgcgg cagcgcggtc ttcgtgcatg actacgacaa gttttcgccg accaaaacgc
    45841 tggagatgtg cgccaaattc ggcgtgacga ctttctgcgc cccgcccacg gtgtaccggt
    45901 tcctgatcaa ggaagatttg tcgaaatatg atttttccaa attggaatac gccgtggtgg
    45961 cgggcgagcc gctcaacccg gaagtgtatg agcaattcct gaaagccacc ggtttgcgcc
    46021 tgatggaagc ctacgggcaa acggaaatga cggtgggcgt gggcaacttt gtgggcatgg
    46081 aacccaagcc cggctccatg ggcaagccgt caccactcta tgaaatgttt ttggcgcggc
    46141 cggatggctc ccggtgcgac ccgggcgagc agggcgaaat cgtggtgccc caagagcgcc
    46201 tgcgcgcgcg cggaatgttt cagggatatc atcgcgaccc ggagggcaac gcccgttgct
    46261 tgcgcggcgg ggtatatcac accggcgacg tggcctggca ggatgaggat ggctatttct
    46321 ggtacgtggg ccgcgacgac gatttgatca agagcagcgg ctatcgcatt ggcccgttcg
    46381 aagtggaaag cgccttgatg gagcatccgg ccgtgatgga gtgcgcagtg accggcgttc
    46441 ccgatgaaga acgcggacag ctcgtcaagg ccaccattgt gctggccaag ggttatgagc
    46501 cctctgacga attacaacac gagttgcagg cgcacgtgaa aaaagtgacc gcgccgtata
    46561 aatatccgcg cgtggtggag tttgtccgcg agttgcccaa gaccatcagc ggcaaaatcc
    46621 gtcgggtgga aatccgcgcg caagacggcg cataatccca tccggcgccg gagcggcgtt
    46681 ccggctagct ttcgcccggt tcctcggctg ccggtaccgc ttggtaggcg gcgaggacct
    46741 ccggcggaat ggctacgggt cgaccggtgc gcagattcag gaacacccaa ctggtttgcg
    46801 cggtagccag cagggtgcca tcgctttcac gcacaaaacg gtatttgcgc aatgaccgga
    46861 ttttttgaaa gttggccacc cacgtctgca ccgcaatggg ttcacccgcg cggcacggtt
    46921 gcagatattc gataaaatgc gagcgcacca cccatgtcca gtcgcggctg tgcagctctt
    46981 cgggagtcca cccgttttgc cgggtgtggg cgatggccga ctcttgaagc aaacgcacat
    47041 attcgatgtt gttgacgtgc tggttttcgt cttccgcttc cggcggaaaa acaaaacgat
    47101 gggtataaac gcgaaccgga tcctttttca tggggtggtg ctcgcgaggc tggtgggcaa
    47161 cgggcaggtg gcgcagagcg agcggtcgtt cagacaatat tcgtggtgaa tatggatgag
    47221 cccttggcgg cgcaaaccgg cgcgataaag ccgtggcgtg tggtcgggcc cgaacaggcg
    47281 cagcgcggtt tgcttgacta attcgttggg ctcctccgcc ggcagatccg ccaacagcgc
    47341 ttgccatgct gaagccggcg cgcccagggc ggccacggcg ggcagcagca gattcacaag
    47401 aatcgcccgg gcgcgtggca aaccaaccag ggccacgggc gcatccaccg gaggcgcgga
    47461 aaatgacagg cgatttgccc aataaggcgt ctccggcaaa tcaaaaagtt gcagcagggt
    47521 attggcgtcg agtccttggg ttcgcttggc aatgtcggca acccgttccg gcaggccggg
    47581 ggtgggggcg aacaattgcg cggccgccat caaccgccgt tcggggcgat tggtcgggcg
    47641 cacaccggca aacacccagt cggcgcgggg aatgcgccgc ggcaaaaatt cgccggacaa
    47701 cggccaccac gcatcccagc aacgccgcaa aaaagatttg gtttcatcat caccgatccg
    47761 gcgtgtggta gggtccggca gcagcgatgc cacacccagc agcagcgcat aggcgcgcca
    47821 cggattgccg cccgattttt cgcgcagtaa ctccagcggc agcacgcgcg ccaaacggcg
    47881 gaacggcgct ttgttgggcc ggtaacccaa agccgccatg acttcttcat agataatttg
    47941 cgccggcccg cgatccagca tggcggcttg caggatttcg gcgcggcggc gcaaccggtg
    48001 ttcgccggcg gcatccagca ccgcgccgcg ctgatcggga gtaagctgca gcatggcggc
    48061 gcggcacggt gggggcgtgg cacgggccgc cacggggtac gaaagcaaat caaggttatc
    48121 aaacgaaaaa tcaagttgcg catccagcgc ggggcgcaag gcaatttgca gtgtgccggg
    48181 cggcagctcg gcttccggca atgtgccggg ataataggat acgtgggcgc acacccgccg
    48241 gtatcgcggg tcgtcggcat ggcggtggcg ttgccaatca gcggggcgca catggatttc
    48301 cacatcgccg gtgatcctgc gcaaatccgg accaacgcgc agcgcggcat tcaggaaatc
    48361 ggggccgggg ccggtgttcc attcaccggg atgctccacc tgcaccggtt cgccggcggc
    48421 ggtagccagc aaggacgggc gcaatcgcgc atcagcccag acgcactgca agtggcgttc
    48481 ggtgaagggg ggcggggggc ggggcggcgc ttcgcagacg ccgcaggaaa gcggtcggga
    48541 actatgcgcg agcgcagccg cccggggaaa ccaatccagc gcgatcccgg tagaaagatt
    48601 catgattctg gtgaataacg gaaagcgccc gtgaagtaaa ctttgaaagc ggccgccaat
    48661 ttcgtatgtt tcgccccgga cgcgcctgta gctcagctgg acagagcatg ggatttctaa
    48721 tcccagggtc gcaggttcga gccctgccag gcgc
//
LOCUS       DAOPNP010000005        47550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_975_length_47550_cov_8.611812, whole genome shotgun sequence.
ACCESSION   DAOPNP010000005 DAOPNP010000000
VERSION     DAOPNP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 47550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47550
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_47550_cov_8.611812"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PKI64_01055"
     CDS             <1..1251
                     /locus_tag="PKI64_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOD99625.1"
                     /translation="PDEIPPIVSMVAPDETALELEPEDGSPFSALAFKIVADKHAGKL
                     TYIRIYSGTLTSGQNVLNSTLDKSQRIGRILRMHANHSENVETAQTGDIVAVVGLAHT
                     RTGDTICDEDHPVHLSTIEFPAPVVSISVAPETTADNEKLSAALYKLADEDPTFTVGF
                     DAETSETIISGMGELHLEIIVDRLKREFGVAAKVGRPEVAYRETATQAVEGQYKHVKQ
                     SGGRGQYAHVCLRLEPLKAGEGFEFVNEVVGGRIPANFIPSVEKGVVKAMSSGPYAGY
                     PIVDMRVTLTDGSYHEVDSSDFAFQEAGRAGFKELFLKGSPIILEPVMSVEVNTPEEF
                     MGGATGSVCQRRGRIETMEEKGSQKLIRGMVPLGEMFGYATALRSMSQGRAGFTMQFE
                     HYEAVPFFLTEQIIKARRAANKIR"
     gene            1883..2467
                     /locus_tag="PKI64_01060"
     CDS             1883..2467
                     /locus_tag="PKI64_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOD99626.1"
                     /translation="MKKIVVFAAALAMAACAFGQAATWMGNSYIWADVDGAGQTWYNA
                     SGTGQSAGAFNGHDFGIVSSLVIGGEVQSYGDADGEANPAFMNYSVDSGSFSAIQLDW
                     FDYASNNNWFQTLTGEDVADGLSVGSHTLAIYFSKPSTDSGAAPDGNLYDSNDSANYN
                     ATFETAAAPVPEPATMSLLGLGALAMVIRRKLSK"
     gene            complement(3103..4581)
                     /locus_tag="PKI64_01065"
     CDS             complement(3103..4581)
                     /locus_tag="PKI64_01065"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOD99627.1"
                     /translation="MYRLVFLSGRHAGKRLMVRQALTTVGSDPDCHLVLTDAPALAPR
                     HAQFKESSAGVFLSALTPEAAILRNGAPLTDTVRLVHDDQLDLGGLLIQFQEIIAPHT
                     RLEPSPGLIQPITLLLAIVILVVEAVMLGFMFDWRTRLITPDAEARDLAWAEEYRAQV
                     EAEKEAKLKNGEAPASSVIVLPGTTDTAPPHATATNHAAEAGTRPDEPPTSAAAAVLD
                     VADFTPATTNTPLANLPPVSAADPAIEAAQRVLAEADAAAQFADFAKAFRLLNQLHQT
                     TPGFLPAYALHARLLENRGDLEGAQYRWGELLTLAPAASPLRQQASTERQRLAQLQEQ
                     QTRILQQPPATDLASLPRNVYFTAPEVQKLLPTADVAEHRLLQTTLELTPNAHLFKNA
                     QIQVFVTFYDKDAAGNIAPSVALVTTDPATLGAAFATTRSVPYTASYVVTHDQRAQAA
                     RDTGQPAAYYGYTLHVFAGAILQDAVAKPKLLLDRGIQFAPP"
     gene            4745..6154
                     /locus_tag="PKI64_01070"
     CDS             4745..6154
                     /locus_tag="PKI64_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOD99628.1"
                     /translation="MATSWITGVCWQQNRLEWTVLRRAKETWEVADAGAADLVSGAAG
                     EAVDWAAAIKPYLKKFKGRVIVALPTERVLLRVALLPSVDALELRGMAELQTDRFSPF
                     PVEMVATGAEVLAAAGTSSLVAMAVVRNDEVAALGQIFQAHGAPPDVVDVAALGWWWG
                     LKTHGQLPAHGTQIILRAAPAGVEMVAVRDGSPLLFRALPGFPPEPAGPAHEEWLAAC
                     VEEVHDSLAALETEWGGSGGPTLHIYHTADWPADCAERLQKSLGLEALYRHPLDELPT
                     VAEGIARRAATAGGGLVMDLAPDAWREADATRRTRRRLLRTATIFLVAWLLGVGGFLT
                     LLNVQRGQLTRWQGQVAELEKPAREIRKLRAKTLEFSQYADRSHSALECLRVVATALP
                     DGVELTKLDYRKGSSVSLRGESDQAQKVYTFIQSLEQVELFPGVKSEGINTRNTPQGE
                     RSQFGVSITLPATGGEDEP"
     gene            6151..6753
                     /locus_tag="PKI64_01075"
     CDS             6151..6753
                     /locus_tag="PKI64_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99629.1"
                     /translation="MKFAARELNLLGLTLGVIVLGLTYLWLEPKFQEWTEFRAEREEL
                     AARRDAAEHLLASRAEVEARLAEFRGGLPVYAVGKKAEAELLLGLEQMAGQHRLTLTR
                     RNAGAERQAGDLFETAITCDWEGDLPALVNFLYAQQSQGAVSDVRQLSVQPVGGRDVP
                     AGHLKGNFTIDYAYRRDAGGAESKADAAPAAPEPPHPEGP"
     gene            6750..9623
                     /gene="gspD"
                     /locus_tag="PKI64_01080"
     CDS             6750..9623
                     /gene="gspD"
                     /locus_tag="PKI64_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOD99630.1"
                     /translation="MMPTRLSLFTRGTTWLLALAFFCASLPAPVLAQDMPPVPSEAEP
                     VEPAEPETAAVDEEPAAPEIEVLSPGAAARERIRQLRAGAGGAEAPAANMIRARTPAG
                     EEAEAVGELYNLNLKEAQLDVLLDRYSELTGRTMIKAPGISATFTFKAQEKLTRAEML
                     QAMDSLLSMNNLALVPLGARFYRVVQIDKAPTEGMAIQKEAPLGGMPDSDMLISQLIS
                     LKYLELEDANTIVQSMLHGYGKVQRFDRIGSLLVTETAANLKRIAEILALVDQPTELK
                     VETRVYEIQHAKAADIAARLNELISGSQGDKPEEVPQVAQAPAATMVRRPANMIRARQ
                     PIPQPEVAMPDSAALEAAMAEKGIIQGKVKVLADERTNIIIVISRPSNFTFFDKIVAV
                     LDRTVEPEVIVQVVALEFADAEEMASLLNDFIGAAKAESETAKPGESRDSTDGRARAL
                     EDFVRARAAAADRVRQAAGEAAVSKIGQLSANTKILADKRTNTLLLMGTKGDIGALQD
                     VIRKVDIMLAQVLIEAVILEVNLNNNTAYGVNWLQKSMTAYNQRMAGANGGVSVREPI
                     TSWGGHFGNNNFSSVAGDTVTRAFGGGTGLSYFFTFADLNIDAVLNMIATSSEGRVLA
                     TPVILTTDNTEASIMSGQQIPIRTGDTTSGGTIQSDYEYKNVGIQLKVKPRINPSRYV
                     TLEITQNADTLGDSVDVGNNGKMQAINKREITASISVPSRSTIVLGGLVQTDYQNANT
                     RVPILGSIPLLGALFRSEDTTRHRTELLVLITPYVLMTPAEARAETERLHGASNVAAE
                     DWYRGWSDSSLAPFSPQRLREMKQEESRQARAKLTTSGEQAPPVALIEPAPPMPKPTP
                     DAQDPETFGREIERAMSALAQQQEADAGLAPADDTPLPAPEEPAAAPYVFDPAVPAEP
                     ATRPAPEKPPRKSRLSPASGRAKKQDEKPSMYPAPQPPNGDTP"
     gene            9620..10078
                     /locus_tag="PKI64_01085"
     CDS             9620..10078
                     /locus_tag="PKI64_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99631.1"
                     /translation="MNEQPTHLNGAAEIREGDILFDCPHCGKSMAIEAAGAGLMVPCA
                     DCGRQVQVPIPDASTPAAPVAAPRPFNGAEDPQVLIEQLDAALAMANQQNDRLIAEKE
                     ALQERRAFLEHLRVTNATRLEQAARELESIQDALDRTMALLAEIRTEKPV"
     gene            10087..11793
                     /locus_tag="PKI64_01090"
     CDS             10087..11793
                     /locus_tag="PKI64_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD99632.1"
                     /translation="MAGTASSRLGALLAATGLFTPEQIEELVGRQREDEGVLTATVVA
                     REYAREDAFLAALAKAMEIPFVRVGDKPIPGEILEKIPTKAVFQSNVIPVGIENGVLR
                     VATSEPFRPGLADALRLAAGMRIKFALSPTADIEKATKKFYGVGADTLDRLMADNQLD
                     LDADSGPAIGDLSNLDQDASVVKFVNQVIWECHHDRGTDIHIEPMETDLRIRYRIDGV
                     LHPTPLPPQMKRFQSAIISRIKVMAGMDIAEKRLPQDGRISLRIKGEEIDVRVSTMPT
                     VYGESVSLRLLMRSSGMFGMDKLGLFPDDAATLNTLIRRPHGILLVTGPTGSGKSTSL
                     YAWLHTINSVDKRILTVEDPIEYEMAGINQIQMKPEIGLTFAVGLRHILRQDPDVIMV
                     GEIRDQETADIAIRAALTGHLVFSTLHTNDAAGGITRLVDMGIEPFLVSSSLEALVAQ
                     RLVRRLCPACKRPWTVDVPFLESLGFPISKLLTGTIYEPVGCEECRGTGYAGRTGIYE
                     ILVMTDAIRQMVVERRAASEIKQHALEHDMHTLRMDGWRKVLAGITTLEEVIRVTSED
                     EA"
     assembly_gap    11991..12090
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12091..13215
                     /locus_tag="PKI64_01095"
     CDS             <12091..13215
                     /locus_tag="PKI64_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD99633.1"
                     /translation="PAATKPRLLGRKEKPRRGWRYEKSIRSRVRMKLSELLLFTHELS
                     DLLASGMTLGAALHALVQRKTGKAQDTIITDLRDEIVSGSSLSAALARWPDSFPSLYV
                     SMVKAGEASGQLPGVLERLVRHYERAQAAREKVSMALVYPLIVTIVGLGAMVFMMTFV
                     IPRFSAMFAELGGTLPLPTRILIGLSNGLIHYGWALLLAILLGGAALRRALRTPAGRE
                     WKDRFLLRAPVAGSIIRANAFANFAHTLGTLLANGVPVLQALAIVENTVGNSIIAKAI
                     NEAKDRVTDGSTISRPLAQDGIFPRLFTDMLAIGEESGDMTGALEHIGRRYDNELDRA
                     VKLFTTILEPIMMLLIAVGVGFVAISMLMAVFELTSGLNT"
     gene            13226..>14043
                     /locus_tag="PKI64_01100"
     CDS             13226..>14043
                     /locus_tag="PKI64_01100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD99634.1"
                     /translation="MMLFDTHAHFDTFAAEGRVAEVLARARAAGVERICAVGSSEASN
                     ELVVQLAQKHPAQLVAAVGYDRDQTDKPRDPARLATLAQAPGVVAIGEIGLDYYYSAE
                     TAPAQRELFGAMLAGAAAARKPVIVHSRYADEDTIAMLGEFSTVWKKLFHTVEKNAET
                     FPHCGKNAPDFSTPWKKVFHSVENLPPAADAPPPPPPGVLHCFTGDAAFAAELVALGF
                     MISFSGIVSFNNAGPLREVARQIPAGQLLVETDCPYLTPKPFRGKVNEPAYVARV"
     assembly_gap    14044..14143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14144..14821
                     /locus_tag="PKI64_01105"
     CDS             <14144..14821
                     /locus_tag="PKI64_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOD99635.1"
                     /translation="AVPLAEMLTQRFGKTARVDNDVNAMAVGECTYGAGQAYQHAVFI
                     TLGTGVGGGLLINKALYRGAYSLAGEIGHVSIDRNGIVSPTGRGGVEQYVGNKRIVER
                     ALQALDAGRTSRILEFAGGRREDITPKVISAAAAAGDELAGEIFDFVADCLATMMASV
                     AYLLQPQAFIIGGGIAAAGPVLFDPLRRHLNERLSPFFAERLVIKHAELGNDAGVIGA
                     ATLTFLE"
     gene            14829..15776
                     /locus_tag="PKI64_01110"
     CDS             14829..15776
                     /locus_tag="PKI64_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD99636.1"
                     /translation="MSRFWKRKRVGLALGSGGARGWAHLGVLQALREKDIQVDLVAGT
                     SMGAVVGAFVAARREDALRELAESLDWRRLRQFFWEISLSRSGLTDGRRLLEETGKLL
                     GVRDFRELALPFRAVATDLYGGGEVVLSSGNLLQALRASISIPGLFSPVVLGRRTLVD
                     GGLVNPVPVSVARDMGAQMVIAVDIVQGPAGVAKPAPRRTSEPSAPAPMLAAPAPAHD
                     EEGDGALNALERFLLDIEKKARRVKASVAEAVSRPAVVDILVQSVRIAESRIALARRQ
                     LEPPDILIEPKVGNIGVLEFQRAQEAMAAGYAAARQALA"
     gene            complement(15884..16153)
                     /gene="rpsT"
                     /locus_tag="PKI64_01115"
     CDS             complement(15884..16153)
                     /gene="rpsT"
                     /locus_tag="PKI64_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOD99637.1"
                     /translation="MPNIKSAIKRAKTSEKSRLRNAREKSQLKTLRKGLLTAAAGAEA
                     PKTMDAYRAFCSGLDKAVKHGIIKKNNATRRKARAAALLRKATAK"
     gene            complement(16236..18221)
                     /gene="nrdJ"
                     /locus_tag="PKI64_01120"
     CDS             complement(16236..18221)
                     /gene="nrdJ"
                     /locus_tag="PKI64_01120"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016050347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HOD99638.1"
                     /translation="MAETYQPSLRAQILTRRTYNRALNEEGTQFETWAQTVDRVIEHQ
                     RWLWKRQLRRPLNKTQEAELEELRGLLLARKVGVAGRTLWLGGTEIAKVREACQFNCA
                     HLEIQTVDDMVDALWLLLQGCGVGVTPKSGGISGFTQPILDVQIIRSTRQDKNGRETN
                     LETWNPETKEWTISVGDTAEAWAKSVGKLLAGKYTAEKLTLDFSEIRPAGTRLTGYGW
                     IGQGDETISVAYRAIIEIMNRRAGQLLRKMDIHDICNWLGTILSTRRSAEISLFEYGA
                     PEWQEFAVCKKDYWSKGQPQRGMSNNSLVFYQKPTRAELRGIFDLMLASGGSEPGFIN
                     GAAALNRAPWFSGVNPCAEILLGNRAFCNLTTIDLAKFKDNPSGMHRAIYIIARANYR
                     QTCVNLKDGILQHSWHENNDFLHLCGVSLTGVVRRPDLGPYELRLLRNAAIMGAYSMA
                     DELGLPRPKNVTTLKPEGTISKCYDTTEGAHKPLARYIFNNVTFVKHDPLVNVLREAG
                     YTIMPHPNGSGDWVITLPVAWDDVEFETVNGLEVNTETAIDQLERYKLLMDNYVEQNC
                     SITVSYAPAEVDAIIEWLLQYWDHYVGVSFLLRADPLKTAADLGYPYLPQQPVTKEVY
                     DAYVASLKPLDLESLKAQSEDAVDMGNDCAGGACPVR"
     gene            complement(18298..19170)
                     /locus_tag="PKI64_01125"
     CDS             complement(18298..19170)
                     /locus_tag="PKI64_01125"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOD99639.1"
                     /translation="MSISVQIIADSLSPQGIRLATLSLKYPRWIHAEFMTHRVFSRNA
                     SSSRAIPSRRLGALEREYPLRWDANIPGMQAGTPLAPEQAARAKAIWDRMADACAAGV
                     AELAELGLHKQWANRPLEWFTSISVLVSATTFDNFFGLRWHPDAQPEIQELARQMYHA
                     LHASTPRRLAPGQWHLPYVAADEEAALGLDTARRCSAARCARVSYLNHDGTTPDIAKD
                     LATFAKLAGDPHGPPRPLHASPMEHQATPDSLRDATDPASWQHPREHGNFHGWRQFRK
                     MLPNEQITTFTPPA"
     gene            complement(19238..20074)
                     /locus_tag="PKI64_01130"
     CDS             complement(19238..20074)
                     /locus_tag="PKI64_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOD99640.1"
                     /translation="MRFWIGVLTSIWLVAAAGAAPAGTFTFTIATANLSDNTSQAYED
                     AGIRILQALKPDVLAIQEFNYKGGNTQDLVRRLFGPSYYFAREKGGARLPNGIISRFP
                     ITAFGQWEDPYVGNRSFAWATIQIPGPKPLHVVSVHLVQNRAERRIPEARHLLGLIRK
                     QFPAGDYVALCGDFNIAGRNALALAELTKWFDDTHHPADQEGNPNTNSTRNRPYDFVL
                     PNPALAPYHVATVLNKQRFPHGMVFDSRLWNPLPPPVEWDDSANNLQHLPVMKTFRVP
                     VR"
     gene            complement(20104..20715)
                     /gene="ruvA"
                     /locus_tag="PKI64_01135"
     CDS             complement(20104..20715)
                     /gene="ruvA"
                     /locus_tag="PKI64_01135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOD99641.1"
                     /translation="MMIAFLDGILEEKEPTRVVINVHGVGYEAAIPLSSYDRLPAPGQ
                     PVRLLTVPVIREDAHLLFGFMSTAERDTFLQLTSVNGIGPKLGLAVLSGLAVRDIKAA
                     IANNDIKRLSSISGIGKKTAERIVLEMRDKLGKGEMMAALAGGEADSPTNAKLRDVLL
                     ALLSLGHKQADAQRMVKDIAGEITPEMPLEDILRKVLSGRHGR"
     gene            21019..21573
                     /gene="thpR"
                     /locus_tag="PKI64_01140"
     CDS             21019..21573
                     /gene="thpR"
                     /locus_tag="PKI64_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOD99642.1"
                     /translation="MNTKRLFAGVKVAATAALRAELAAWQQALRGERIRWTRPENLHL
                     TIEFFGATEEGRIPELKAALAQAAAATKTFAIKLGGAGTFGGARSPRVLWLGVESAGL
                     EELHGRVVAALAGIGWNPEAREFAPHLTLGRISRLQDTRRFGEALESRRAGVLPEQPV
                     NELVLFASVTGRYVPVGSWGLGGG"
     gene            complement(21603..23117)
                     /locus_tag="PKI64_01145"
     CDS             complement(21603..23117)
                     /locus_tag="PKI64_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD99643.1"
                     /translation="MASPANAVAPPAAARAFDFRSLWLVLLFAFIGAASFQGSRGLYE
                     STEGRYAETARETMLSGDYDDPILNLQPHWTKPPLTYVAIIYGLRLFGENPWGVRAYL
                     VFALVLAAGAVWLAGSSIWGSGAGRWAGIIFATSPFIALAAHSVTTDMLVALWTAGAL
                     AAFWHGYASHCRLSLLGMWLFLALGLLTKGPPALLVPAVCLPVAWIHLRRANDWRPGR
                     WNLGLGLSIFILLGLGWYFVEARLNPGLTSYWLGKEMIARNFTSEMHRNPNAWFVFFV
                     YLPILVAGTGPWLPLALFRARPLKAWLAASDQRRTWNRAARRSLLAGVAVPFVLFSIS
                     QSRLALYLVPLFVPLCLLLGRMIDVLIAQGRLRARVAGGCAGVLLAGIVIAKSATLFI
                     TSSQDMTRLSAILAPVLARHEEHALCSISNQRLNGLEFHLRRPIEAVDPDAIWDHMKA
                     TALGGRPTDYLVKNARWARLATNAPARVHAEPVGPHWLLVAPEPAAWREKFKTP"
     gene            complement(23133..24077)
                     /locus_tag="PKI64_01150"
     CDS             complement(23133..24077)
                     /locus_tag="PKI64_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOD99644.1"
                     /translation="MLDLKSKRIVVTGGAGFLGSFILERLRQRGCRDNVFVPRIEEYD
                     LVEHANVQRMYDDFRPDLVIHLAAVVGGIGANRAHPGEFYFKNMAMGLFLMEEARRRK
                     IEKFVALGTICAYPKFTPVPFREEDLWNGYPEETNAPYGLAKKMMLVQSQAYRQEYGF
                     NSIFLLPVNLYGPRDNFDPASSHVIPALIKKCLDARDVGAREIVVWGTGNASREFLYA
                     GDAAEGIVLAAERYNSSEPVNIGAGFEIKIKDLVPLIARLTGFQGDIVWDATKPDGQP
                     RRMLDTSRAEKLFGFKAKVGFEEGLRETIAWYEKSRAP"
     gene            24178..25434
                     /gene="thrC"
                     /locus_tag="PKI64_01155"
     CDS             24178..25434
                     /gene="thrC"
                     /locus_tag="PKI64_01155"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOD99645.1"
                     /translation="MKTHFNGFRCFACGAEQGADYAGYVCPQCGGNLEVRYDWPRHAP
                     GRWWLEPARRDIFRYQALLPVSRLELASPLRVGQTPLYDAPRLSAATGLKHLLLKDDG
                     QNPSASFKDRAGAVALVRAREIGAQVLCGASTGNAGSSMACLAASVGMPCVIFVPEKA
                     PAAKIAQLLVFGAKVFAVRGTYDDAFDLCMEVCRQRGWFNRNTGHNPFTREGKKTAAI
                     ELYEQLGRMPDWVVVPTGDGNIIAGIWKGFQDLKEAGIVARTPKMLCAQSEASQAISA
                     AVWALQQTGQTKPDWAKVQVAAVQATTVADSISVDIPRDGLAAVRAVVESGGAAVAVP
                     DAEILAAIPELARSAGVFAEPAAACAWAGLKKAARDGLVQPDETVVCMLTGNGLKDVA
                     SARKVAGEPVALDNNINAALAATENI"
     gene            complement(25545..25736)
                     /locus_tag="PKI64_01160"
     CDS             complement(25545..25736)
                     /locus_tag="PKI64_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99646.1"
                     /translation="MNGPEKIALAWLVRRNTSMRTTWIKARLGMGTATCFAAFLKKLE
                     EARKGDWGHDAWRTVRSIQ"
     gene            complement(25808..26770)
                     /locus_tag="PKI64_01165"
     CDS             complement(25808..26770)
                     /locus_tag="PKI64_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD99647.1"
                     /translation="MSRGNRLQKIFRGDADCAMFLTTLGEVCQRTGWRVHAYTLLGNH
                     YHLLLETPEPNLVVGMQWVQSTYTKRFNARHRERGHLFQGRYKAIPVEAGSGYFSTLA
                     TYIHLNPVRIRGYDFDKQRLVDYAWSSYPAYVGKAAQPEWLNFHRVLSELGMADTPAG
                     RRKYAGYMAGRVEEVRHSAQPWKADEAWQRIRRGWHMGGEEFRQELLERVGGALKNAR
                     RQSFSGGEVQAHDEACAEQWIAKGLKALGITEAELAGLRMNGPEKIALAWLVRRNTSM
                     RTTWIKARLGMGTATCFAAFLKKLEEARKGDWGHDAWRAVRSIQ"
     gene            26927..28180
                     /locus_tag="PKI64_01170"
     CDS             26927..28180
                     /locus_tag="PKI64_01170"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOD99648.1"
                     /translation="MDMHTHLLSNRLAVVVWRGVFWAVVLLALGGCSKHRAGDAPRPV
                     DPDLAHPVLNYAAIAPGVEHAEYSRTQPAPLSYHVVRADLTQGGLSLAVLRTAASSGS
                     ERGSLLEMARAIETPERRTVAAISGDYFGNGMAGTWGILLVDGRLRYSPSGRSALLID
                     ANGRPIIDRPQSRIQMQVGDDPAWFEIRDMNRPGQGEEPGLHLYARSSEVTHVPAPKG
                     AVAIAADLPLAGGDVVGTVTGTFAAEDSFRLPETGLVLAGAGDATKLPAGLREGASVR
                     IRTTIMPAAREAVGGGPRIVRDGKVSIELEPDGICAAEASYLKRRHPRAVVGLAGGGQ
                     TVLMVLVRGRCNESEGIKLDDLADLLVGLGAADAIMFDGGDSATIVENDSYVIQGRAG
                     ARHMCDGLAIQAPRTPEQAVAGSAP"
     gene            28177..28800
                     /locus_tag="PKI64_01175"
     CDS             28177..28800
                     /locus_tag="PKI64_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99649.1"
                     /translation="MSVRSLSRLALILGGALLALSFFLPLGYHSPGEEFLATVDSLTS
                     AESAGEFVVAGGLLIVIAYSYIWAVLVALAALWNFAGKRRAWTPVHVAVQSIGGLTLM
                     TLCILLLSLHDPWLPAKFQWTGASLPLVMLVPLWAVALLAAPVRREWVVVAVGMFPQV
                     LFQFMLAGVSLSREGQAHGFVIGGIGALLALAGAIGRARMGASLIGK"
     gene            complement(28787..30874)
                     /locus_tag="PKI64_01180"
     CDS             complement(28787..30874)
                     /locus_tag="PKI64_01180"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOD99650.1"
                     /translation="MTDPVWQSRGARRFLVAPGWDHPDSLPTLDQLPYLVEKEGAPLV
                     PPGRHRVDRLTLPLGGGAVDAVVKTYAPQAAWRDRLARRAGSKAERAFRTALALQQAG
                     VGTPAPLAVVERWEGARLRECRFVARHEPDLTNLRDELNRIYAHAPLCAPLMDLLQDV
                     ADAVRALHDAGILHRDLGNQNIALRPTPNATRPWQVLFLDLNRAAHAANPTPAQRGAD
                     LARLDLPSDFRRVFHAMYFHGYAPPAEFARAEQRTRAAFARHTALRPLRHPCREARIR
                     REKAAQPQPLRGREIWIWDDRSAQAIPAYTPRDRRKLLPAANVVAALHSAVVKGWPIQ
                     REYRRLRQESFTRPVPFANAIGMTLEPEPDTWDRQLEWLAQLQGGHPMPLLLRLYHHQ
                     GPAAWQWTLDQARHLHRQGHLVSLALVQDRRAIRDPKGWRQMVSMAFDRAHSYADFFE
                     VGHAINRAKWGVWDFREYRQLLQPVHACSIQYPEAKITGPACIDFEPYVLGALLGGIP
                     AEQRPHALSHHLYVDRRGAPENPQGPFDTVAKCALLRAYARAYGLTEDRILISEVNWP
                     LLGTGVWSPVNSPYETRDPRQNDPSVSEADYAAFMARYLLLTLASGHVSRVYWWRLAA
                     RGFGLIDDTDPAAWRPRPAFNLLQTMLAQLDGATFGRRLDTPPGDYALEFTRPAAPPL
                     TVRWSPATAPTFQ"
     gene            complement(30867..31706)
                     /locus_tag="PKI64_01185"
     CDS             complement(30867..31706)
                     /locus_tag="PKI64_01185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD99651.1"
                     /translation="MLVSTRVLRHIDPTMRPDHLKISACVTAGNEEDKIAACLASLTW
                     CDEVIVVDSFSTDRTFEISRQYTPHVYQHAWEGYIAQKNYIRTLARYPWILFVDADEV
                     VSDELRQEIEAEFARGPGEIVAYQFPRMVYYLGKWIRHGEWYPDIKLRLFLKDRGHSA
                     GEEPHDRVVVDGPIKTLRFPLHHFTYDDVTDHLNTLNRFSTISAAQKYARGEKFRWRD
                     LVFRPPWRLFKSLFIKRGILDGRQGLIIAVMTTFGVFIKYAKLMELWIKHHQPQGDHG
                     HHD"
     gene            complement(31712..32797)
                     /locus_tag="PKI64_01190"
     CDS             complement(31712..32797)
                     /locus_tag="PKI64_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD99652.1"
                     /translation="MKARWLAVWTLLMPALAHAAPPLRVACVGDSITYGDQLTDRDTQ
                     AYPVVLQRLAPERYLTGNFGVNGATALHNSYLAWTDTPACRAAQDFAPDIVVIMLGIN
                     DLSYPDHAASYADDLLALTRLWQAQPIPPRLFLCTLTPLAPAFLQGKANRTIRNVMNP
                     AIRTVAAQAGAGVIDISANFPNRLALLPDGVHPSAQGAELIARTVLAALDAATAPPPQ
                     IQPAPEAGPVAISVRNEALAARDRAAQWLAANPAPPSADAEPAADPAPLLPLLVAPLA
                     DAPPGLYSSLAALAAALAEAGQRIVFITDDQPVAWREALLHQLVQRQKIATDGTGYWT
                     DTDSADISAATRATHYALRALALALGE"
     gene            complement(32794..33015)
                     /locus_tag="PKI64_01195"
     CDS             complement(32794..33015)
                     /locus_tag="PKI64_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99653.1"
                     /translation="MIIRLYWITRILGYAASLVGVFLYLHHQADAAGDGRNLSFAIIG
                     AGFVSFFISYAIRAWLRFGARAQRDIPGP"
     gene            33165..33764
                     /locus_tag="PKI64_01200"
     CDS             33165..33764
                     /locus_tag="PKI64_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD99654.1"
                     /translation="MNRTSRRARGFTLIELLVVLAIIGVLATILLPAINGAMKKGKVA
                     RCKTEVADLEGAIKRFYAEYNKMPLPKGSKFGDAENGFTDLQQAQIIQVLLNLDDGGW
                     PGGKRNTKQMPFLDISPSAFWIASQNRYAKTIVEMEAALASGQPYCDPWKQPYGILMD
                     LNMDDRITGSKFAPEIRAKVAVYSIGENGDDNNPLYRTW"
     gene            33794..34339
                     /locus_tag="PKI64_01205"
     CDS             33794..34339
                     /locus_tag="PKI64_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOD99655.1"
                     /translation="MKAGRQQRQGGFTLIEMLVVIAIIGILAAITVPGVGKARKVALK
                     RRAMMEMNSIKLAVHQFYDDHRYMPWGDPNAPNQARVGDDVWTASGADQARVIQWLTG
                     DNPKKKAYLQVPDKSRPSASSFQFNDPWGEYYRIGMDRNMDGAIRPDGGSGGGDYVRD
                     QVLVYSLGDPDARPRTPLKTW"
     gene            34390..34935
                     /locus_tag="PKI64_01210"
     CDS             34390..34935
                     /locus_tag="PKI64_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD99656.1"
                     /translation="MKAQQPRTRTAARQGFTLVEILVVITILGILMAMMVPAAGLIIK
                     RAKVSQAKSDAGVVVAIMMKYRAEYNRWPSFAKSQGTLRTDGDWVKAMSPKPGGGFDE
                     NNPKRIVFFQPGAGALDETTGAFVDPWGQPFVYMLDIDGDGQMDDPSGSAQKMGASIL
                     AWSAGPDQDETTFEDNPVSWK"
     gene            34932..35666
                     /locus_tag="PKI64_01215"
     CDS             34932..35666
                     /locus_tag="PKI64_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD99657.1"
                     /translation="MSTRCNNRGFTLIELLVVVAIIGVLFAVALPVFENAGRKDTEKA
                     AFNLITTMRLARQHAISKRQWVLVVFPNQDGGNYDESGPNGIDKCLRAYAAIAVTNNM
                     DGFDRPAQIPSNMKYAFISDWKYLPEGTYFDDDETLNGNYLFKERGTDFRYPLDPARP
                     NSSPTRPMGVVMFRPNGRAYKFAGTTGTGKYWQDTDYSKIYVTSAKYYERQNGRLVGP
                     NAIPGGTNTAVEIRNKTGQVHIWDGN"
     gene            35677..36279
                     /locus_tag="PKI64_01220"
     CDS             35677..36279
                     /locus_tag="PKI64_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99658.1"
                     /translation="MKHTANPKSTSRAGYSLVEVTLALLVVAIGLTATFALFPEGLRA
                     TRSAVDDAEISLFADYVFTTLDLTAAKYGGNYGPNWTIAKTDDFISLMLSTLPSAQDS
                     FELKDGGRTANFYWMPDYYGLDPGKFSGTDYNANSFRSAKFWTSAFTYTLEIVLGTWK
                     GHGQGVTTCAVLKVWPGEYSGSGKPKGEPRTFYREIIPIR"
     gene            36297..37235
                     /locus_tag="PKI64_01225"
     CDS             36297..37235
                     /locus_tag="PKI64_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD99659.1"
                     /translation="MKQATHGTPRASAARVGHRQGFTLIEVLAAMAVLVILVLALTRM
                     FMEATSITRRGTSALLRNSTAETAMETLLQDAEGMVVNDRLACYVKADTADPNGFGFD
                     DVWFISTSGDQDDDLPYEYFHYFVTNKIYTNSLGAPYVRFDLMKERMIFAVGDDIKYG
                     SGTRFYAMETNTVQWWTRARNMPGQVWDRQVLADNVVRFDVYCTGWDGQAWMGGFSGV
                     HEFDSTRGPRGNIALAGIPPAAFDIYLQVTSPDTAIESGMALMAGVPNDVQTKAREKM
                     IRDSSSLFGRAVPIIGAARYVTVTTYGTNNTVYYED"
     gene            37225..39690
                     /locus_tag="PKI64_01230"
     CDS             37225..39690
                     /locus_tag="PKI64_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99660.1"
                     /translation="MKISSDLHVRSGRPAATRRNQQGVALIVVLGFLSIMIIMALAFL
                     TQARVERQVASATLEAMRGRQILRTALNAAMNDYSVELAAHQLVMPPVNTDYDMFTSI
                     PPKPIMGMGGRTIGQDNIELLVGEVEDWIPRKYFNPPYNAKTRVPANAEWILVRDDPS
                     SSQPSRILGRYAYVCFDMSGGVDGNLVARESEVAGQDARVASNRIRRSVRQMPMGLLP
                     EAVNPSQFKSYRRGWKGFDSLQTLIKLTDGKAQDGNDGHTRWQPERKEIFGAGLASNL
                     VSDVVPFSLSVYRGGRYSRSSATWSKPEFCGAAPWTTVLSPIQSQFAPGWQAWVSNAI
                     MDFTSSSPYPIGTDYPSPKNVPMFNEINMSYRLLTPEQDPVSGWYKYTVEVTLTVEFW
                     YPFPSEDNVRSGTYNMPPPTLTGGAAAGDGQIWMQMVMPTVAPPLVAFDPSAAPPAAL
                     SVEAKYNNGRPYLATGTTNFVYKIPLIRPVPPSPAPPDYNPAYPFPNNVSPLLVNMVQ
                     VRKPIYLRAGTNNVDMIPPGLTIQPVAANVMPGAPVTKVYVQEVTDPRLNHIPGQWVL
                     QGAPSLEARNTWGGSTLNKFMAEGTNLYCRNAPMETPADLGYISTGKEWETIDICTPA
                     AADVLANLIADPNLYATFSSNKVFYTNGTINPNTRSSNVLACAFYDLATQETPNTKTE
                     VVGANPIDEATARLLAQSIMDSTVSGTIDNACQAGSDWVRCAAMQQGGALAAIPLNNN
                     QRESLVRNTWGLFSPDNSLFTVVVVAQTIKEGPNNVGTWNAADDVITGERRAVALVWR
                     DPYKNGVNPHHEMLVRMFRYLND"
     gene            complement(40382..45490)
                     /locus_tag="PKI64_01235"
     CDS             complement(40382..45490)
                     /locus_tag="PKI64_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD99661.1"
                     /translation="MRKKAGIIAMALLGAAGMAGAASSPYSSMAVAGSAFTNVWSSTP
                     NMTLVANNTWVTTQTVTSGSGEFKFTANGTWTTNWGGAAAVARVPAVGRAPVPGGGNL
                     SFTDLTPGLYRFTFNDSTLEFRMEWAGASPLPLPAFANMAVVGDFNNWQPNINSQLTN
                     HPAPQDYLWSGSLTLESSTAFQFQPDENPNIQWGGTEAQTLAWPITNGNAGGKSSFTL
                     AGFAPGTFFFTLNVSNASFSIVQTATQEVVVTTMAAQGNFISMAAPPANMMRVGNSSQ
                     WTADYHITNNGPVTLRFAANNGAAIWGVTNGTPNFTLPVSGTMQNALTNYATINAVTP
                     GRYGITFDHVSGAFSVRRMYADNSGINLLKNPGFELTTQPDGGDAVDWGSWQAWPKRV
                     ADGFGPHAGNWCGAVHGKWFEEWTDYGSFAQDVAVTSGKTYSASAWLKATPNWSASTM
                     QIKIEWLDVTNGIAGPDVIRDISSLTTNWVKYSVEGAAPNTATKAHVVFLCSGATTNS
                     GATMQVDDAEFRVVASRSQNFDSWGSLASYGAFAPDWSITSGKTVYNVQPGRPAADVF
                     ISQYIEGTGNNKAIEIYNGTLSNLDLAAQNYVLQQYDNGSTTATVTIALSGVLQPGTT
                     LVVGRPSTPAEYKPDDAITGQLRLQTNKYLTFNGDDVIVLRKGGASGTVKDRVGQVGT
                     NATGSAWSRNTQDHTLTRKGTIFTGTVSSVTAAFPLVEEWIISGKDDFTGLGTHDISY
                     IDPNQPYTPAGYSLIMNTNATLMSGELSGGVGDVSFWWRTESMSPAVTMVIETASSES
                     GPWITNATLASVAASNFAYYVAAINRADATYLRIRQTGGATNRFRIDEVVISELTGTR
                     RTEDFLGWVDPSYTIPGNYARYGWAIQNASITTNGLSQTRAALLGASNSCVVSPSFDG
                     GLGETRFWARAADFSETAYLVLQTTTNAGSNWTTQASFTATTGTTFSAWLFMDAPNAQ
                     ARIISDPSKPFADAIVDSIEIRLPMIYRNQNFDGWPVKSQYGTGVSLFQGWAITDCIV
                     DSQNADVGQVARLNSVVGNYIVSPYLRDGLGPVSFRTRKWAASDTNPTLQMQVSPDGT
                     TWTTLATINIQSITYETTTLFLYDTSNHYVRLYHSAGASRVLVDDIRIGTPQPRPEVM
                     VVPGLSPAPLVDTPLTLAADIVTRYGASIVSVTGRYRIANGAWNTVPMVPGEHFGNYV
                     ATSTIPGQSAGTRIMYTVTVQYAGIGAVTNLPGYATNQLTTATRTNFISAVQQGSVWI
                     NELGYASYSTNEPFVFLDEDPYFAWTGQNHEFVEICGLAGSDLSGWKLQLALGADLDI
                     AANSNQPIYATYTIPNGTILSNQNNGFGFYVIGDAELAGSNPVNQNLTTLVPLSVQPF
                     AVEYKDHIHDGVGVIRLVDLYGNLIYSLSYVGYASGADRIPQSQTPQDTNSVGLIGTG
                     SAYPDFGWELGDLTIGEPNDGQVLQEPQPTNVWAYAWHDQQTKVTPLNTNVAAAFYML
                     DPLNAANWDIVGVYYGYTNNAYLSPTGTLYHRLGDSGNPWTQVAMSLREGSQDADGYS
                     YVYAQIQPRTYRRLDTMEYFIAVYPNKAGVQPVFLGSDDGNDNLSTVYTNWAGAELHP
                     FHHQFPIADRIFITNMVLGTTNVILQTEGNDFFDPVTNFYVRFTTNLLMPINSWTSTN
                     FTHTLNAASQSMFNVKWSTNVKPRAFYRVDPHGP"
     gene            45718..47406
                     /locus_tag="PKI64_01240"
     CDS             45718..47406
                     /locus_tag="PKI64_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99662.1"
                     /translation="MKKTALASLWILVGLAVTAWGQATLPTFHSGPWYGQPLPTGWTQ
                     SGLGTDYKTDYDKDGGFAAKFDGSGDWLQIRYNSAASTVSYYAMGNGTSGEYVYKVQE
                     SVNGASWTDVVTFNSGNPLPTSAPAQYTNSLNAASRYVRFIYVTKATGNVGLDGVLVS
                     GPSLPTITFTPNGTNSAPVSNQFSMAVAITPGGAGLKSWSMAPAYAGPATLTNGTFTF
                     TPAATDNGKTFTVSVVASNVVGTITNTANITVSAYVPPVPVIQFSPAAPYSVMATQTQ
                     KLGIAVSPLGSGIASWTLLPAYSGTATLTGTNFSFIPATNDAPANYTLTVLATNTFGT
                     TTGTATIAVAAYVPPPPPAAYICTFEDGSKTGYASGDVTLSNKVWNLTGILIGSDAAD
                     LKIGNKAARLKYDPTDGDDVMTIQSPVMSNGISTISLWYGPYGNHGDTAPTLAIEVSE
                     NLSSGWIEVGRVNAGAVEVLTYYSADVFINTPLYVRIRAVDGNGGRSCNFDNITITPF
                     AIQPVSPYDAFLLKYNVTPGDPGTLPGDDYDGDGFTNQQEFDANTNPYDDAVHP"
BASE COUNT         8779 a        14047 c        15890 g         8634 t
ORIGIN      
        1 cccgacgaaa ttccgccgat cgtctccatg gtcgcgccgg acgaaacggc gctggaactc
       61 gaacccgaag acggttcgcc gttcagcgcg ctggcgttca agatcgtggc cgacaagcac
      121 gccggcaaat tgacctacat ccgcatctat tccggcacgc tgacgtcggg ccagaacgtg
      181 ctcaacagca ccttggacaa gagccagcgc atcgggcgca tcctgcgcat gcacgcgaac
      241 cattcggaga acgtcgagac ggcgcagacg ggcgacatcg tggcggtcgt cggcctggcc
      301 cacacgcgca cgggcgacac catttgcgac gaagaccatc ccgtccatct cagcacgatc
      361 gagttcccgg ccccggtggt gtcgatttcg gtcgcgccgg aaacgacggc ggacaacgag
      421 aagctctccg ccgcgctcta caagctggcg gacgaggatc cgaccttcac ggtcggtttc
      481 gacgccgaaa cgagcgagac gatcatttcg gggatgggcg agctgcacct ggagatcatc
      541 gtggaccgcc tcaagcgcga attcggcgtg gcggcgaagg tggggcgccc ggaagtggcc
      601 taccgcgaga cggcgacgca ggcggtcgaa ggccagtaca agcacgtcaa gcagagcggc
      661 ggccgcggcc agtacgcgca cgtctgcctg cgcctggagc cgctgaaggc gggcgagggc
      721 ttcgagttcg tcaacgaggt cgtcggcggg cgcatcccgg ccaacttcat tccctcggtg
      781 gaaaagggcg tggtcaaggc gatgtcgagc gggccctacg cgggctatcc gatcgtggac
      841 atgcgcgtga cgctgaccga cgggtcgtac cacgaagtgg attcgtccga cttcgcgttc
      901 caggaagcgg gccgcgccgg cttcaaggag ctgttcctga agggcagtcc gatcatcctc
      961 gagccggtga tgtccgtgga agtgaacacg cccgaagaat tcatgggcgg cgccacgggc
     1021 agcgtgtgcc aacggcgcgg gcgcatcgag acgatggaag agaagggctc gcagaagctg
     1081 atccgcggca tggtgcccct gggcgaaatg ttcggctacg cgacggcgct gcgcagcatg
     1141 agccagggcc gcgcgggctt cacgatgcag ttcgagcact acgaagccgt gccgttcttc
     1201 ctgaccgagc agatcatcaa ggcccgccgc gcggccaaca agatccggta ggatgggggg
     1261 gggacaagat tcaatcttca atttccaagt gaacagccgc tgcgcccagc gggcgcggcg
     1321 catctgcgat tcggtgcatg cctgcaaaaa aagccgtggt cctgcaagac tgtcatcccg
     1381 agccttgcct gcccgccgga gccttgcgcg aaggagggtc gagggatctc agcccggtcc
     1441 cgcaggctga cccgcgggat tgtgcccaga tatttcgact ccggcgctgc gcgccttcgc
     1501 tcaatatgac ccccaaaggg tgccccgaat cgcagatgcg ccccagcggg cgcggcttca
     1561 gcgacttgat cattggatat tgaagattga agattgagat ttcctcccgg cggcttgggc
     1621 cgagtcgccc ggcctgccga tccggcgccg attccatggc ggcttccggc ggaatgcggc
     1681 aagaaaagac aaaaaaccgg gaaaaaatcg ggccgcaatc cgttcgcgtc cagcgggttg
     1741 cggaaccggc tccgtaaaaa ggtatatttt gagcaaaaaa cgcttgaccg acccgtgctt
     1801 cttggcctag tgtgcgcacc gtcacggaat atcgtggctc ggtttaaaaa ataaataaac
     1861 aaatccgaaa aggagaatga cgatgaagaa gattgtcgtg tttgcggcgg ctcttgcgat
     1921 ggcggcttgc gcctttggcc aggcggcgac ctggatgggc aacagctaca tttgggcgga
     1981 tgtcgatggc gcgggccaga cttggtataa tgccagcgga accggacaat ccgccggcgc
     2041 gttcaatggt catgatttcg gcattgtgag ttccttggtc attggcggcg aggttcagtc
     2101 gtatggcgac gctgatggag aggccaatcc cgccttcatg aactacagcg tggatagcgg
     2161 atctttctcg gcgatccagc tggattggtt cgattacgcg agcaacaaca actggttcca
     2221 aacccttacg ggcgaagatg tggccgatgg gttgtcggtg ggatcgcaca ccttggcgat
     2281 ttacttctcc aagccttcga cggatagcgg cgctgctccc gatgggaacc tctacgacag
     2341 caacgacagc gcgaactaca acgcgacctt cgagacggcg gccgctccgg ttccggagcc
     2401 cgcgacgatg agcctgctgg gcctcggcgc cttggcgatg gtcatccgtc gcaagctgag
     2461 caagtaatcc tttccccagg atgaaacgaa agagcggctg gcaacagccg ctcttttcgt
     2521 ttgggggtaa tgggattttc tgtggccggc agcggtgagg ctggtgttcg gaaaaaatct
     2581 cctctcgcca agccgccaag ggcgccaaga agaagaagca tcttaacgca atcgccgcaa
     2641 aagagcgcag gcgtggtcat gatgtcggcc gccgtagtac cggcggcggg atccaaaaaa
     2701 acgagcagga aaacaggaga tgcaggaaag caggaaaggg ccggagacac caacgcaagc
     2761 gccgcaactg ccgcaagaga gcgcaaacgc agagagaggg aaagggggct agggcttggt
     2821 tttgggaatc ggattttctg tagccggcct cgctgaggcc ggggcaaaaa acagcccacc
     2881 ggggttttca accccgaccg accaaagccg acgcgcgggt tcgttgtcgg ccgccgctgt
     2941 gccgccggcg gaatttggag ccagaacaga cggaacagcc gagcaggaaa tcaggaaaag
     3001 aaaaaagtac ctggctgaaa acctgttttg tgtttaccgg ttttcttgcg tctgttgcgt
     3061 cgcttgcgtt ggatgtctta cgttgggcca ggtggggaat gtttaggggg gggcgaactg
     3121 gatgccgcgg tcgaggagga gcttgggttt ggcaacggcg tcttgcagga tggcaccggc
     3181 aaagacgtgg agggtgtagc cgtaataggc ggcgggttgg ccggtatcgc gggcggcctg
     3241 ggcgcgctgg tcgtgagtga cgacgtagct ggcggtgtaa ggcacgctgc gggttgttgc
     3301 aaaggcggcg cccagggtgg ccgggtcggt ggtgacgagg gccacggaag gggcgatgtt
     3361 gccggcggcg tctttatcgt agaaggtgac gaacacctgg atttgggcgt ttttgaagag
     3421 gtgggcgtta ggggtgagtt ccaacgtggt ttggagcaag cggtgctcgg cgacatcggc
     3481 ggtggggagc agcttttgca cttcgggggc ggtgaagtat acgttgcgcg ggaggctggc
     3541 gaggtcggtg gcgggaggct gttgcaggat gcgggtttgc tgctcttgca gttgggccaa
     3601 gcgctggcgc tccgtgctgg cttgctggcg caaaggcgat gcggcaggag cgagggtgag
     3661 tagttcaccc cagcggtatt gcgcgccttc gaggtcaccg cggttttcca gcaggcgggc
     3721 gtggagggca taggcgggga ggaagccggg cgtggtctgg tggagctggt tgagcaaccg
     3781 gaatgccttg gcaaagtcgg cgaactgggc ggcggcatcg gcttcggcca agacgcgctg
     3841 ggcggcctcg atggcgggat cggcggcgga aacggggggc aggttggcaa gcggggtgtt
     3901 ggtggtggcg ggggtgaaat cggccacgtc caacacggcg gcggcggcgc tggttggtgg
     3961 ctcgtcgggg cgggtgccgg cttcggcagc atggttggtg gcagtggcgt gggggggggc
     4021 ggtatcggtg gtgccgggca ggacaattac ggagctggcg ggggcttcgc cgtttttgag
     4081 tttggcctct ttttcggctt cgacctgcgc gcggtactcc tcggcccagg ccagatcgcg
     4141 cgcttcggca tccggtgtga tcaggcgggt gcgccagtcg aacatgaacc cgagcatgac
     4201 agcctccacc accaaaataa cgatggccag caggagggtg atgggctgga tcaacccggg
     4261 gctgggttcg agccgcgtgt ggggcgcaat gatttcctga aactggatga ggagcccgcc
     4321 gagatcaagc tgatcgtcgt ggacaaggcg cacggtatcc gtgagtggcg cgccattgcg
     4381 caagatggcg gcttccggcg tgagcgcgga aagaaacacg ccggcactgc tttccttgaa
     4441 ctgcgcgtgg cggggggcaa gggcgggggc atcggtcagc accaaatggc aatcgggatc
     4501 gctgcccacc gtggtgagcg cttggcggac catgaggcgc ttgcccgcgt ggcgtccgga
     4561 tagaaaaacc aacctataca ttccgggggg aagtgtggaa caagccccgc ggcttggcaa
     4621 cctgaaatcg gcgcgcggcg cgggcgcggt tgagttttcc ggcggcgggc cgcggaatcc
     4681 ggttgcttgc gggcggcggt gcagttagga tgccattatt ggtatagggt agagattgcg
     4741 caacatggca acttcgtgga tcaccggcgt ttgctggcag caaaaccgtt tggaatggac
     4801 ggtcttgcgg cgtgccaagg aaacgtggga ggtggccgat gccggcgcgg cggatctggt
     4861 atccggcgcg gcaggggagg ccgtggattg ggccgccgcg atcaagcctt atctcaagaa
     4921 gttcaagggg cgggtgatcg tggcgctgcc gacggagcgg gtgctgctgc gcgtggcgct
     4981 gttgccgagc gtggacgcac tggaattgcg cggcatggcg gaattgcaga cggaccgctt
     5041 ctcgccgttc ccggtggaga tggtggccac gggcgccgag gtgctggcgg cagcgggcac
     5101 gtcctcgctg gtggcgatgg ccgtagtgcg caacgacgaa gtggcggcgc tggggcagat
     5161 ttttcaggcg catggcgcgc cgccggatgt ggtggatgtg gcggcgctgg gttggtggtg
     5221 ggggctcaag acgcacggcc aactaccagc gcacggcacc cagattatcc tgcgcgccgc
     5281 gcccgctggc gtggaaatgg tggcggtgcg cgatggttcg ccgctactct tccgcgcatt
     5341 gccgggattc ccgccggagc ccgccggacc cgcgcacgag gaatggctgg cggcctgcgt
     5401 ggaagaagtg cacgattcgc tggcggcgct ggagacggag tggggtggca gcggcgggcc
     5461 cacgctgcat atttatcaca cagccgactg gccggcggat tgcgcggagc ggttgcagaa
     5521 atcattgggg cttgaagccc tctatcggca tccgctcgat gaactaccga cggtggccga
     5581 gggcattgca cgccgcgcgg cgacggctgg aggcgggttg gtgatggact tggcgcccga
     5641 tgcctggcgc gaggccgatg ccacgcggcg cacgcgccgc cgcctgctgc gcaccgccac
     5701 gattttcctc gtggcctggc tgctgggcgt gggcggcttc ctcacgctgc tcaacgtgca
     5761 gcgcggccaa ctgacgcgct ggcaggggca ggtggcggag ttggaaaagc ccgcgcgcga
     5821 aattcgcaag ctgcgcgcca agacgctgga gttttcgcaa tatgccgacc gcagccactc
     5881 cgcgctggaa tgcctgcgtg tggtggccac cgcgctgccg gatggcgtgg agctgaccaa
     5941 gctcgattat cgcaaggggt cttcggtatc gctgcggggc gaatccgacc aagcgcaaaa
     6001 agtctacacc ttcatccaga gcctcgagca ggtggagctg ttcccggggg tgaagtcgga
     6061 aggcatcaac acgcgcaaca cgccgcaggg cgagcgctcg cagttcggcg tgagcatcac
     6121 gctgcccgcc accggcgggg aggatgagcc atgaagttcg ccgcgcggga gctgaatctg
     6181 ctgggtctga cgctgggcgt gattgtcctg ggcttgacct atttgtggct ggagcccaag
     6241 tttcaggaat ggacggagtt tcgcgccgag cgcgaagaac tcgcggcgcg ccgcgatgcg
     6301 gcggagcacc tgctggccag ccgcgcggaa gtggaggcgc gcttggcgga attccgcggc
     6361 ggcctgccgg tctatgcggt gggcaaaaaa gcggaggcgg aattgctgct gggcttggaa
     6421 caaatggcgg gacaacaccg cctgacgctg acgcgccgca acgcgggcgc ggaacggcag
     6481 gccggcgact tgttcgagac ggccatcacc tgcgactggg aaggtgactt gcccgcgctg
     6541 gtgaacttcc tctatgcgca gcagtcacag ggcgcggtgt cggacgtacg ccaactgtcc
     6601 gtgcagcctg ttggcgggcg cgatgtgccg gccggccacc tcaaaggaaa ctttacgatt
     6661 gattacgcgt accgccggga tgccggcggc gcggagagca aagcggatgc cgcccccgcg
     6721 gcgccggaac caccccaccc ggaaggccca tgatgcccac tcgcctgtcg ttattcaccc
     6781 gtggcaccac ctggttgctg gccctggcct ttttctgcgc ctcgctgccg gcgccggttt
     6841 tggcgcagga tatgccgccg gttccgtcgg aggccgaacc ggtcgaaccg gctgagccgg
     6901 agacggcagc cgtggacgaa gagccggccg cgccggagat cgaggtcctc tcgcccggcg
     6961 ccgccgcgcg cgagcgcatt cgccaattgc gcgccggggc tggcggagcc gaagcgcccg
     7021 cggccaacat gattcgggcg cgtacccccg ccggcgagga agccgaagcg gtgggcgagc
     7081 tgtataacct taatctcaag gaagcgcagt tggatgtgct gctcgaccgc tactccgaac
     7141 tgaccggccg caccatgatc aaggcgccgg gtatctccgc caccttcacc ttcaaggcgc
     7201 aggaaaaact cacccgcgcc gaaatgctgc aagccatgga cagcctgctc tccatgaaca
     7261 acctcgcgct ggtgccgctg ggcgcgcggt tttaccgcgt ggtgcagatt gacaaggcgc
     7321 ccaccgaggg catggccatc caaaaggaag cgccgctggg cggcatgccc gacagcgata
     7381 tgctcatcag ccagctcatc agtctgaaat atctggagct ggaagacgcc aacaccatcg
     7441 tgcaaagcat gctgcacggc tacggcaagg tccagcgctt tgaccgcatc ggcagcctgc
     7501 tggtgacgga aaccgccgcc aacctcaagc gcatcgccga aatccttgcg ctggtggacc
     7561 agcccaccga gctgaaagtg gaaacgcgcg tctatgaaat ccagcacgcc aaggccgccg
     7621 acatcgccgc gcgccttaat gagctgatca gcggcagcca gggcgacaag ccggaagaag
     7681 tgccgcaggt cgcgcaggcg cccgccgcca ccatggtgcg ccgccccgcc aacatgatcc
     7741 gcgcccgcca gcccatcccg cagccggaag tcgccatgcc cgacagcgcc gcgctcgaag
     7801 ccgccatggc ggaaaaaggc ataattcagg ggaaggtgaa agtgctggcc gacgaacgta
     7861 ccaacatcat catcgtcatt tcgcggccgt ccaacttcac gttcttcgac aagatcgtgg
     7921 cggtgctcga ccgcaccgtg gagccggaag tgattgtgca ggtcgtcgcg ttggagttcg
     7981 ccgacgccga agaaatggcc agcctgctca atgatttcat tggcgcggcc aaggccgaga
     8041 gcgaaaccgc caagcccggc gaaagccgcg acagcaccga tggccgtgcc cgtgcactgg
     8101 aagattttgt ccgcgcccgc gccgccgccg ccgaccgtgt gcggcaggcc gccggcgaag
     8161 cggcggtcag caagatcggc cagctttcgg ccaacaccaa gattctggcg gacaagcgca
     8221 ccaatacgct gctgctgatg ggcaccaagg gcgatatcgg cgcgctgcag gacgtcatcc
     8281 gcaaagtaga catcatgctg gcgcaggtgt tgatcgaagc cgtgatcctg gaagtgaacc
     8341 tcaacaacaa cacggcctac ggcgtcaact ggctgcagaa gtccatgacc gcctacaacc
     8401 agcgcatggc cggtgccaat ggcggcgtga gcgtgcgcga gccgatcacc tcgtggggcg
     8461 gccacttcgg caacaacaac ttctcctccg tcgccggcga caccgtcacc cgcgccttcg
     8521 gcggcggcac ggggttgagc tacttcttta cgtttgccga tctcaacatt gacgcggtgc
     8581 tcaacatgat tgccacctcc agcgaggggc gcgtgctagc cacgcccgtc atcctcacca
     8641 ccgacaacac cgaagccagc atcatgagcg gccagcagat tcccatccgc acgggcgaca
     8701 ccaccagcgg cggcaccatt cagtcggact acgaatacaa gaacgtcggc atccagttga
     8761 aggtcaagcc acggatcaat ccgtcgcgtt acgtcacgct ggaaatcacg cagaacgccg
     8821 acacgctcgg cgacagcgtg gatgtcggca acaacggcaa gatgcaggcc atcaacaagc
     8881 gcgaaatcac cgcctccatt tccgtgccga gccgctcgac catcgtgctg ggcggcttgg
     8941 tgcaaactga ttatcagaac gccaacacgc gcgtgccgat tctgggcagc attccgctgc
     9001 tcggcgcgct gttccgcagc gaggacacca cccgccaccg caccgagctg ctggtgctca
     9061 ttacccccta cgtgctgatg acccccgccg aagcccgcgc cgaaaccgaa cgcctccacg
     9121 gcgcctcgaa tgtcgccgcc gaggattggt atcgcggctg gtcggacagc tcgctggcgc
     9181 cgttctcccc gcagcgcctg cgcgaaatga agcaggaaga aagccggcag gcgcgtgcca
     9241 aactgaccac cagtggcgag caggcgcccc ccgtggcgct gatcgagccc gcgccgccca
     9301 tgcccaagcc gacgcccgat gcccaagacc ccgaaacctt tggccgtgaa atcgaacgcg
     9361 ccatgagcgc gctggcccag cagcaggagg ctgacgccgg cctcgcgccg gcggacgata
     9421 cgccgctgcc cgcgccggaa gaacccgccg ccgcgcccta tgtgttcgat cccgccgtgc
     9481 ccgccgagcc agccacccgg cccgcgccgg aaaaaccgcc ccgcaaatcg cggctgtccc
     9541 cggcgagtgg ccgcgcgaaa aagcaggatg aaaaaccctc catgtatcct gcgccgcaac
     9601 cccccaacgg agacacgcca tgaacgaaca acccacccat ctcaacggcg ccgcggaaat
     9661 ccgggaaggc gatattctct ttgactgccc gcactgcggc aaaagcatgg ccattgaggc
     9721 cgccggcgca ggcctcatgg tgccctgcgc cgattgcggt cgccaagtcc aggtgcccat
     9781 ccccgatgct tccacgcccg ctgcgccggt ggccgctccg cgccccttca acggcgcgga
     9841 ggacccgcag gtgctgatcg agcagttgga tgccgcgctg gccatggcca accagcagaa
     9901 cgaccgcctg atcgccgaaa aagaggcgct gcaagaacgc cgcgcatttc tggaacatct
     9961 gcgcgtgacc aatgccacgc gcctcgaaca ggccgcccgc gagctggaaa gcatccagga
    10021 cgcgctggac cgcaccatgg cgctgctggc cgaaatccgc acggagaaac ccgtctaggg
    10081 cgcgcgatgg ccggaacggc ttccagccgc ctcggggcct tgcttgcggc caccgggctg
    10141 tttacccccg agcaaatcga agagctcgtg ggtcgccaac gcgaagacga aggcgtgctg
    10201 accgccaccg tcgtcgcccg cgaatacgcg cgcgaggatg ccttcctcgc cgcgctcgcc
    10261 aaggccatgg agattccgtt cgtccgcgtg ggcgataaac ccatccccgg cgaaatcctg
    10321 gaaaagattc cgaccaaggc ggtcttccaa tccaacgtca ttcccgtcgg catcgagaat
    10381 ggcgtgctgc gcgtggctac cagcgaaccc ttccgcccgg gcctggccga tgccctccgc
    10441 ctcgccgccg gaatgcgcat caagttcgcg ttgagcccca cggccgatat cgaaaaggcc
    10501 accaaaaagt tttatggcgt cggcgcggac acgctcgacc gcctcatggc cgacaaccag
    10561 ttggaccttg atgccgattc cggccccgcc attggcgacc tgagcaatct ggatcaggac
    10621 gccagcgtgg tgaagttcgt caaccaggtc atttgggaat gccaccacga ccgcggcacg
    10681 gatatccaca ttgagcccat ggaaacggac ctgcgcatcc gttaccgcat tgacggcgtg
    10741 ctgcatccca cccccctgcc gccgcaaatg aagcgcttcc agtccgccat catcagccgc
    10801 atcaaggtca tggccggcat ggacattgcc gaaaagcgcc tgccgcagga tggccgcatc
    10861 agcctgcgca tcaagggcga ggaaattgac gtgcgcgtgt ccaccatgcc caccgtctat
    10921 ggcgaaagcg tcagcctgcg cctgctcatg cgctccagcg gcatgttcgg catggacaag
    10981 ctgggcctct tcccggacga cgccgccacc ctcaacaccc tcatccgccg cccgcacggc
    11041 atcttgctcg tgaccggccc caccggctcc ggcaaatcca ccagcctcta cgcctggctc
    11101 cacaccatca actccgtgga caagcgcatc ctgaccgtcg aagaccccat tgaatacgaa
    11161 atggccggca tcaaccaaat ccagatgaag ccggaaatcg gcctgacctt cgccgtcggg
    11221 ctgcgccaca ttttgcgcca ggacccggat gtcatcatgg tcggggaaat ccgcgaccag
    11281 gaaacggcgg acatcgccat ccgcgcggcg ctcaccggtc acttggtgtt cagcacgctg
    11341 cacaccaacg atgccgctgg cggcatcacg cgccttgtgg atatgggcat cgagcccttc
    11401 ctcgtttcca gctcgctgga agcgctggtg gcgcagcgcc tcgtgcgccg cctgtgcccc
    11461 gcctgcaagc gcccgtggac cgtggatgtg cccttcctcg aaagcctcgg cttcccgatt
    11521 tccaagcttc tcaccggcac catctatgaa cctgtcggct gcgaagaatg tcgcggcacc
    11581 ggttatgccg gccgtaccgg catctatgaa attctcgtga tgaccgatgc catccgccag
    11641 atggtggtgg agcgtcgcgc cgcttcggaa atcaagcaac acgcgctgga acacgacatg
    11701 cacacgctgc gcatggacgg ctggcgcaag gtgctcgccg gcatcaccac gttggaggaa
    11761 gtgatccgcg tcacgtcgga ggatgaagcc tagccggcgc ccgccgcccg cgcgccgcag
    11821 ccgccatggc caagtttcgc tacatcgcca aaacccgcac cggcgagcgc caggaaggcg
    11881 tgctggaggc ccccgaccgc cgcgcgctgc tggcccaact gggccgcgcc ggcctcgtgc
    11941 ccatcgccgt gcaggatgtg caacccgccg cgcccgcccc ggcccccgcc nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccgccgcca ccaagccgcg gctgcttggc
    12121 cgcaaggaaa aaccccgccg cggctggcgc tacgaaaaaa gcatccgctc ccgcgtgcgg
    12181 atgaagctct ccgagctgtt gcttttcacc cacgaactct ccgacctgct tgcctccggc
    12241 atgaccctcg gcgccgcgct gcacgcgctg gtccagcgca aaaccggcaa ggcgcaggac
    12301 accatcatca ccgacctgcg cgacgaaatc gtctccggct ccagcctctc cgccgcgctg
    12361 gcccgctggc cggacagctt cccctcgcta tacgtcagca tggtcaaggc cggcgaagcc
    12421 agcggccagt tgcccggcgt gctggaacgc ctcgtgcgcc actacgagcg cgcgcaagcc
    12481 gcccgcgaaa aagtcagcat ggccctcgtc tatccgctca tcgtcaccat cgtcggcctc
    12541 ggcgcgatgg tcttcatgat gacgttcgtc atcccgcggt tctcggccat gttcgccgaa
    12601 ctcggcggca cgctgccgct gcccacccgc atcctgatcg gcctcagcaa cggcctcatc
    12661 cattacggct gggcgctgct gttggccatc ctgctcggcg gcgcggccct gcgccgcgcg
    12721 ctgcgcaccc ccgccggccg cgaatggaag gaccgcttcc tgctgcgcgc gccggtggcc
    12781 ggcagcatca ttcgcgccaa cgccttcgcc aacttcgcgc acacgctggg cacgctgctc
    12841 gccaacggcg tgccggtgct gcaagcgctg gccatcgtgg aaaacaccgt gggcaactct
    12901 atcattgcca aggccatcaa cgaggccaag gaccgcgtga ccgatggctc caccatctcg
    12961 cgcccgctgg cgcaggacgg catcttcccg cgcctgttca ccgacatgct cgccatcggc
    13021 gaagaatccg gcgacatgac cggcgcgctc gaacacatcg gccgccgcta cgacaacgaa
    13081 ctcgaccgcg ccgtcaaact cttcaccacc atcctcgaac cgatcatgat gctgctgatt
    13141 gccgtcggcg tcggttttgt cgccatcagc atgctgatgg ccgtcttcga gctgaccagc
    13201 ggcctcaaca cctgagcccc cgccgatgat gctcttcgat acgcacgcgc attttgacac
    13261 ctttgcggcg gaggggcgtg tggcggaggt gttggcccgt gcgcgcgcgg cgggggtgga
    13321 gcgcatttgc gccgtgggca gctcggaagc ctccaacgag ctggtcgtgc aattggccca
    13381 aaagcatccc gcgcagcttg tggccgccgt gggctacgat cgcgaccaaa ccgacaagcc
    13441 gcgcgacccc gcgcgcctcg ccacgctggc ccaggcgccc ggcgtggtgg ccatcggcga
    13501 gatcggcctc gattactatt attccgcgga gactgcaccc gcccagcgcg agctgttcgg
    13561 cgccatgctg gccggcgccg ccgccgcgcg caagcccgtc atcgtccaca gccgctacgc
    13621 ggacgaggat accatcgcca tgctcggcga gttttccaca gtgtggaaaa aacttttcca
    13681 caccgtggaa aaaaatgccg aaacttttcc acactgtgga aaaaatgcgc ccgatttttc
    13741 cacaccgtgg aaaaaagttt tccacagtgt ggaaaacctg ccgcccgccg cagacgcgcc
    13801 gcccccgccg ccaccgggcg tgctgcattg tttcacgggc gatgccgcct ttgccgccga
    13861 attggtggcg ctggggttca tgatcagttt tagcggcatt gtctccttca acaacgcggg
    13921 gccgctgcgc gaggtggccc gccagattcc cgccgggcag ttgctggtgg aaacggattg
    13981 tccgtatttg acacccaagc cctttcgcgg gaaagtgaac gaaccggcgt atgtcgcgcg
    14041 cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgtgc cgctggcgga
    14161 aatgctgacg cagcgcttcg gcaaaaccgc gcgggtggat aatgacgtca acgcaatggc
    14221 cgtgggcgag tgcacgtacg gcgcggggca ggcctatcag cacgcggtat tcatcactct
    14281 tggcacgggg gttggtggcg ggctgctgat caacaaggcg ctttaccgcg gcgcgtattc
    14341 gctggcgggc gagatcggcc atgtgtccat tgaccgcaac ggcattgtgt cgcccacggg
    14401 gcgcggcggg gtcgagcagt atgtgggcaa caagcgcatt gtggagcgcg cgttgcaagc
    14461 gctggatgcg ggccgcacga gccgcattct ggagtttgcc ggcggccggc gcgaggatat
    14521 cacccccaag gtcatcagcg ccgccgcggc ggcaggtgat gaacttgccg gtgagatttt
    14581 tgattttgtg gccgactgcc tcgccacgat gatggcttcg gtggcctatc tgctgcagcc
    14641 gcaggcgttc atcatcggcg gcggcattgc ggcggcgggg ccggtgctgt tcgatccgct
    14701 gcgccgccat ttgaatgaac ggttgagccc gttctttgcc gagcggctgg tgatcaagca
    14761 cgccgaattg ggcaacgatg ccggcgtgat cggcgcggcc acgctgactt tcttggagtg
    14821 aggcggtcat gagccggttc tggaagcgca agcgggtggg tctggcgctg gggagcggtg
    14881 gcgcacgtgg ctgggcccat ctgggcgtgc tgcaagcgct gcgcgaaaaa gatattcagg
    14941 tggatctcgt ggccggcacg agcatgggcg cggtggtcgg cgcgtttgtg gccgcgcggc
    15001 gcgaggacgc gctgcgcgag ctggcggagt cgctcgactg gcgtcggctg cggcaatttt
    15061 tctgggaaat ctcgctctcg cgctcaggcc tgaccgatgg ccgccgcctg ctggaagaaa
    15121 ctggcaagtt gctgggcgtg cgcgattttc gcgagctggc gctgccgttt cgcgcggtgg
    15181 ccacggacct gtacggcggc ggcgaagtgg tgctcagttc cggcaacctg ctgcaagcgt
    15241 tgcgcgcgag catttccatc cccggactgt tttccccggt ggtgctgggg cgccgcacac
    15301 tggtggatgg cgggctggtg aatcccgtgc cggtgagcgt ggcgcgcgat atgggcgcgc
    15361 agatggtgat tgccgtggat attgtgcagg ggcccgccgg cgtggctaag cccgccccgc
    15421 gtcgcaccag cgaaccgtcg gcgccggcgc ccatgctggc cgcgcccgcg cccgcccacg
    15481 atgaagaagg cgatggcgcg ctgaatgcgc tggaacgctt cctgctcgac atcgaaaaaa
    15541 aggcgcggcg cgtcaaggcc tcggtggcgg aggcggtgtc gcgcccggcg gtggtggata
    15601 ttctggtgca gtcggtgcgc attgcggagt cgcggattgc gctggcgcgg cggcagcttg
    15661 agccgccgga tattttgatc gagcccaagg tgggcaatat cggcgtgttg gagtttcagc
    15721 gcgcgcagga agcgatggcg gcgggttacg ccgcggcccg ccaagcgctg gcctgatggg
    15781 tcgccgcgcg gcggatgccg gtgcggcgcg ggcaaacaaa caccccgcgc gccggggggc
    15841 gggcggggcg tggacggtca cgcggaccga agagccaaac cgcttacttg gcggtggctt
    15901 tgcgcagcag cgccgcggcg cgcgctttgc ggcgcgtggc gttgttcttc ttgatgatcc
    15961 cgtgcttgac ggctttgtcg aggccggaac aaaacgcgcg gtaggcgtcc atggtcttgg
    16021 gcgcttcggc gccggccgcc gcggtcaaca ggcccttgcg cagggtcttg agttgcgact
    16081 tttcccgggc gttgcgcagg cgggacttct cagatgtctt ggcgcgcttg atcgcgctct
    16141 tgatgttggg catggttgct tactcctctt ccaattgaaa aagctcggcc agtctagcgg
    16201 cagggcgggg gatgtcaatg gtttttccgg ccgtttcagc ggacggggca ggcgcccccg
    16261 gcgcagtcgt tgcccatgtc caccgcatct tcggattgcg ccttgaggct ttcgaggtcc
    16321 agcggtttga ggctggcgac gtaggcgtca tagacttcct tggtgacggg ctgctgcggg
    16381 aggtaggggt agccgaggtc ggcagcggtt ttgagcggat cggcgcgcaa caggaacgac
    16441 acgccgacat aatgatccca gtactgcagc agccactcga tgatggcatc cacttccgcg
    16501 ggggcgtagc tgacagtgat ggagcagttt tgttcaacgt agttatccat gagcagcttg
    16561 tagcgttcga gctggtctat ggcggtctcg gtgttgactt ccaagccgtt gacagtttca
    16621 aactccacgt catcccacgc cacgggcagg gtgatgaccc agtcgccgga accgttgggg
    16681 tggggcatga tggtgtagcc ggcttcgcgc aggacgttga cgagcggatc gtgtttcaca
    16741 aacgtgacgt tgttgaagat gtagcgcgcg agcggtttgt gagcgccttc ggtggtgtcg
    16801 tagcacttgg agatggtgcc ctcgggcttg agggtggtga cgttcttggg gcgcggcagg
    16861 ccgagttcat cggccatgga ataggcgccc atgatggcgg cgttgcgcag caggcgcagc
    16921 tcgtaggggc cgaggtcggg gcggcgcacc acgccggtga gcgacacgcc gcacaggtgc
    16981 aggaagtcgt tgttctcgtg ccacgagtgc tggaggatgc cgtccttgag attcacgcaa
    17041 gtctggcggt agttggcgcg cgcgatgatg taaatggcgc ggtgcatgcc gctggggttg
    17101 tccttgaact tggccagatc aatcgtggtc aggttgcaga aggcgcgatt gcccagcagg
    17161 atttccgcgc aggggttcac accggaaaac cacggcgcgc ggttcagcgc ggccgcgccg
    17221 ttgatgaagc ccggctcgga accgccgctg gcgagcatca gatcaaaaat accacgcagc
    17281 tccgcgcgcg tcggtttttg gtagaagacg agggagttgt tggacattcc gcgctgcggc
    17341 tggcctttgc tccagtaatc ttttttgcac actgcgaatt cctgccattc cggcgcgcca
    17401 tattcaaaca ggctaatttc cgccgagcgg cgcgtgctga gaatggtgcc cagccagttg
    17461 cagatgtcgt ggatgtccat cttgcgcagg agctggccgg cgcggcgatt catgatttca
    17521 atgatggcgc ggtaagccac ggagatggtt tcatcgcctt gcccaatcca gccgtagccg
    17581 gtcaaccgcg tgccggcggg gcggatttcg gaaaaatcga gagtaagttt ttcggcggta
    17641 tatttgccgg ccaggagttt gccgacggat ttggcccacg cttcggcggt gtcgcccacg
    17701 ctgattgtcc attccttggt ttcgggattc caggtttcca agttggtctc gcgcccgttt
    17761 ttatcctgcc gcgtggagcg gatgatttgc acatcgagaa ttggctgggt gaagccgcta
    17821 atgccgccgg acttgggcgt gacgcccacg ccgcagccct gcagcagcag ccagagcgca
    17881 tccaccatat cgtccacggt ttggatttcc aaatgtgcgc agttgaattg gcaggcctcg
    17941 cgcaccttgg cgatttccgt gccgccgagc cacaacgtgc ggccggccac gccaaccttg
    18001 cgcgccagca gcaggccgcg gagttcttcc agctcggcct cctgcgtttt gttcagcggg
    18061 cggcgcagtt ggcgtttcca tagccagcgc tggtgctcaa tgacgcggtc cacggtttgc
    18121 gcccaggttt caaactgggt gccttcttca tttagggcgc ggttataggt gcgtcgcgtc
    18181 aggatttgcg cgcgcagcga gggttggtag gtttcggcca tggtggtctc caaaggggtg
    18241 gtagcgtcaa aaaaatcagg cgaattctag ggcaaacgac cgggtgagaa aagcgcctta
    18301 tgcgggcggg gtgaaggtgg tgatttgttc gttgggcagc attttgcgga actgccgcca
    18361 gccgtggaaa ttgccgtgct cgcgcgggtg ctgccacgag gcgggatcgg tggcgtcgcg
    18421 caggctatcg ggggtggctt ggtgctccat gggcgaggcg tggagcgggc gcggcgggcc
    18481 gtgcggatcg ccggccagct tggcaaatgt ggcgaggtct ttggcgatgt ccggcgtggt
    18541 gccgtcgtgg ttgagatagg aaacgcgggc gcagcgcgcc gccgagcagc gccgcgcggt
    18601 gtcgaggccg agggcagctt cttcatcggc ggccacgtag ggcaggtgcc attggccggg
    18661 cgcgaggcgg cgcggcgtac tggcgtgcag cgcgtggtac atctgacggg ccagctcctg
    18721 gatttccggt tgggcatcgg ggtgccagcg caggccgaaa aagttatcga acgtggtcgc
    18781 cgacaccagc accgaaatgg aagtgaacca ctccagcggg cggttggccc attgcttgtg
    18841 caggcccagc tcagccagct ccgccacgcc ggcggcgcag gcatcggcca tgcggtccca
    18901 aatggccttg gcgcgggcgg cctgctcggg ggcgagcggc gtaccggcct gcatgccggg
    18961 gatgttggca tcccagcgca gtggatattc gcgctccagc gcgccgagcc gccgcgaagg
    19021 gatcgcgcgc gagctgctgg cgttgcggga aaaaacgcgg tgggtcatga attcggcgtg
    19081 aatccaacgg gggtacttca acgagagggt ggccagccgg atcccctgcg gcgaaaggct
    19141 gtcggcgatg atctgcacgg agatgctcat ggcctatggt gtagcgcatc cgctggccca
    19201 ggaaaaggac attgtcccgc cggcggcggg agggcattca gcgcaccggg acgcggaagg
    19261 ttttcatcac cggcaaatgc tgcaaattgt tggcggaatc atcccactcg accggcgggg
    19321 gcagcggatt ccacaggcgc gaatcgaaaa ccatcccgtg cggaaaacgc tgtttgttca
    19381 gcacggtggc cacatggtac ggcgccagtg cggggttggg cagcacaaag tcgtaaggcc
    19441 ggttgcgcgt ggaattggta ttggggttgc cttcttgatc cgcggggtgg tgcgtatcgt
    19501 caaaccactt ggtcaattcg gccagcgcga gggcattgcg gcccgcgatg ttgaaatcgc
    19561 cgcagagcgc cacgtagtcg ccggcgggaa attgcttgcg gatgaggccc agcagatggc
    19621 gggcctccgg gatgcgtcgc tcggcacggt tctgcaccaa atgaacgctg accacatgca
    19681 gtggcttcgg gccggggatt tggatggtgg cccacgcaaa actgcggttg ccaacgtagg
    19741 ggtcttccca ctgcccaaac gccgtgatgg gaaaacggct gatgatgccg ttgggcaggc
    19801 gggcgccgcc tttttcccgc gcgaaatagt agctggggcc gaacagccgt cgcacgaggt
    19861 cctgcgtgtt gccgcccttg tagttgaatt cctgaatggc cagcacgtcc ggctttagtg
    19921 cctgcaaaat ccggatgccg gcatcttcgt aagcctggct ggtgttatcc gagaggttgg
    19981 ccgtggcaat cgtgaaggtg aacgtgccgg ccggcgccgc ccccgccgcg gccacgagcc
    20041 aaatgcttgt cagtacacca atccaaaaac gcatgagaag aaggaggata ccctaaagcc
    20101 ggcctagcgt ccatgccgcc cggacaaaac tttgcgcagg atatcttcca acggcatctc
    20161 cggcgtgatt tcgcccgcga tatccttgac catgcgttgg gcgtcagcct gcttgtggcc
    20221 gagggaaagc agtgcgagga gcacatcgcg cagcttggca ttggtggggc tgtcggcttc
    20281 gccgccggcc agcgccgcca tcatttcgcc cttgccaagc ttgtcgcgca tttccagcac
    20341 gatgcgttcc gcggtttttt tgccaatgcc ggaaatgctg ctcagccgtt tgatgtcgtt
    20401 gttggcgatg gcggccttga tgtcgcgcac cgcgaggccg gacaacaccg ccagcccgag
    20461 tttggggccg atgccgttca cggaggtcag ttgcaaaaaa gtgtcgcgtt cggctgtgct
    20521 catgaagccg aagagcaggt gggcgtcttc acggataact ggcacggtca gcaggcgcac
    20581 gggttggccg ggggcgggca ggcggtcata gctgctaagc gggatggcgg cttcgtagcc
    20641 cacgccatgc acattgatga ccacgcgggt gggttccttc tcctccaaaa tgccatccag
    20701 aaacgcaatc atcatgcacc atgctttcct gcgccacatc taaccacgca aagacccgcc
    20761 gcgtccagag ggttgggcgg gtcttggcgg ctgtccgacc ggcggacgtg gcccggtggt
    20821 caaatcaggc gtttgctttt ttggcgagcc gggcgcgttc cttgagccgt ttgacgcggc
    20881 gatcacgggc tttgcgcttc actcgttttc ttaattgttg tcccataagg ctgtccgtcg
    20941 tatcaaaagc ggggaggccg agtcaagagc ggcgcattcg gatttgaaag cagggacggg
    21001 atttggaaca gtcggcgaat gaacacgaag cggctattcg cgggggtgaa ggtggcggcg
    21061 acggcggcgc tgcgggcgga actggcggcg tggcaacagg ccctgcgggg ggagcggatt
    21121 cgctggacga ggccggaaaa cctgcatctg accattgagt ttttcggggc gacggaggag
    21181 ggccggattc cggaactgaa ggcggcgctg gcgcaggccg cggcggcaac gaagacgttc
    21241 gcgatcaaac tgggcggggc ggggacgttc ggcggcgcgc ggagcccgcg ggtgttgtgg
    21301 ctgggtgtcg aatcggcggg gctggaagaa ctgcatggac gggtagtggc tgcgctggcg
    21361 gggattggtt ggaatccgga ggcgcgggaa ttcgcgccgc acttgacgct ggggcggatc
    21421 agtcgcctgc aagacacgcg ccggttcggc gaggcgctgg aaagccgtcg cgccggagtc
    21481 ctgccggagc agccggtgaa cgaactggtt ttgttcgcga gtgtgaccgg gcggtacgtg
    21541 ccggtcggga gctggggact gggcgggggc tgacgttttc ataacccgcc ccggagaggg
    21601 tactacgggg ttttgaattt ctcccgccag gcggccggct caggggcgac gagcagccaa
    21661 tgcgggccga ccggttcggc atggacgcga gccggggcat tggtcgcgag gcgtgcccag
    21721 cgggcatttt tcacaaggta gtccgtcggc cggccaccca aggccgtggc tttcatgtgg
    21781 tcccagatcg cgtccggatc aacggcttcg ataggccggc gcaggtggaa ttccaatccg
    21841 ttcagccgct ggttgctgat cgaacataaa gcgtgttctt cgtgcctggc gaggacgggc
    21901 gccagaatgg cggaaaggcg ggtcatgtcc tgggaactgg tgatgaacag cgtggcggat
    21961 ttggcgatga cgatgcccgc gagcagaacg cccgcgcacc cgccggcgac ccgggcgcgc
    22021 aggcggccct gggcaatgag cacatcaatc attcgcccga gcaacaggca caacgggacg
    22081 aaaaggggaa ccaggtataa ggcgagccgg gattggctga tggaaaacag gacgaatgga
    22141 accgcgacgc cggccaggag agagcggcgc gcggcccggt tccacgtccg gcgctggtcg
    22201 cttgcggcca gccaggcctt cagcggacgg gcgcggaaca gggccagcgg gagccatggg
    22261 ccggtgcccg ccacgaggat ggggagatag acgaaaaaca caaaccatgc gttgggattg
    22321 cggtgcattt cgctggtgaa gttgcgcgcg atcatttcct tgcccagcca gtaggaagtc
    22381 aggccggggt tgagccgggc ttcgacgaaa taccagccca gacccagcag gatgaaaatg
    22441 ctcagtccga gtcccagatt ccaacggccc ggccgccaat cattggcacg gcgcaggtga
    22501 atccaagcga cgggcaggca gacggcggga accaagagcg cggggggacc cttggtgagg
    22561 aggccgaggg cgagaaatag ccacatgccc agcagggaaa gccggcaatg gctggcgtag
    22621 ccatgccaga aggccgcaag ggctccggca gtccacagcg ccacgagcat gtccgtggtc
    22681 acggaatggg cggccaaggc gatgaacggg gaggtggcaa aaataatgcc ggcccagcgt
    22741 ccggcgcccg aaccccagat ggaggagccc gccagccaaa ccgcgcccgc cgccagaacc
    22801 agcgcgaaga ccagataggc gcgcacgccc cacggatttt cgccgaacag gcgtaagcca
    22861 tagatgatcg ccacatacgt gaggggtggc ttggtccagt ggggttggag gttcaggatg
    22921 ggatcgtcgt agtcgccgga aagcatggtt tcgcgggcgg tttccgcata gcggccttcc
    22981 gtggattcgt agagtccgcg gctgccctgg aatgaagccg cgccgatgaa ggcgaagagg
    23041 agcaccagcc acaaactccg gaaatcgaac gcgcgcgcgg ccgcaggggg agcaaccgcg
    23101 ttcgcgggag aagccatgag gcgccaggcg cgctaaggag cgcggctttt ctcgtaccac
    23161 gcgatagttt cgcgcagacc ttcctcaaag ccaaccttgg ctttgaagcc gaaaagtttt
    23221 tcggcgcggg aggtgtccag catccggcgc ggttggccgt cgggcttggt ggcatcccac
    23281 acgatgtcgc cttggaaacc ggtgaggcgc gcgatgagcg gcacgaggtc cttgattttg
    23341 atttcaaagc cggcgccgat gttgacgggc tcggaggagt tgtagcgctc ggcggccagc
    23401 acgatgcctt cagcggcgtc gccggcgtag agaaactcgc gcgaggcgtt gcccgtgccc
    23461 cacacaacga tttcccgcgc accaacatcg cgggcatcta ggcacttttt gatgagcgcc
    23521 ggaatgacgt gcgaggaagc cggatcgaag ttatcgcgcg gcccgtaaag gttgacgggc
    23581 agcaggaaga tggagttgaa gccatattcc tgccggtaag cctgcgattg gaccagcatc
    23641 atttttttgg cgagaccgta gggcgcgttg gtttcttcgg gatagccgtt ccagagatct
    23701 tcctcgcgga agggcacggg ggtgaacttg ggataggcac aaatggtgcc aagggccacg
    23761 aacttttcga ttttgcgtcg gcgggcttcc tccatcagga acaggcccat ggccatgttc
    23821 ttgaagtaga actcgccggg gtgcgcgcgg ttggcgccga tgccgccaac cacggcggca
    23881 aggtggatga ccaggtcggg gcgaaagtcg tcatacatcc gttgcacgtt ggcgtgctcc
    23941 accaggtcgt attcctcgat gcggggcacg aaaacgttgt cgcggcagcc gcgctggcgg
    24001 agccgctcca gaataaagct gccgaggaaa ccggcgcccc cggtcacgac gatgcgcttg
    24061 gatttgaggt cgagcatggc gttctcctga aagcgcaaaa tagcccaatg gccgggcggt
    24121 ggaaagggaa aaggtggagt tgccaccggc ggcgatgccc ggcatactgg cggggctatg
    24181 aagacacatt tcaatggttt ccggtgtttt gcgtgcgggg cggagcaggg ggcggattat
    24241 gcgggctatg tctgcccaca atgcggcggc aatttggagg tgcgctatga ctggccccgg
    24301 cacgcgccgg gtcgttggtg gctggagccg gcgcggcggg atattttccg ctatcaggcg
    24361 ttgttgccgg tgtcgcggtt ggagttggcc tcgccattgc gtgtggggca aacgccgtta
    24421 tatgacgcgc cgcggctgag cgcggccacc ggcctcaagc acctgctgct gaaagatgac
    24481 gggcaaaacc cgagcgcgtc attcaaggat cgggcggggg cggtggcgtt ggttcgcgcg
    24541 cgcgaaatcg gcgcgcaggt gctgtgcggc gccagcaccg gcaacgcggg cagttccatg
    24601 gcctgcctcg cggctagcgt gggaatgcct tgcgtgattt ttgtgccgga aaaagcgcct
    24661 gccgccaaga tcgcccagtt gcttgtgttc ggggccaagg tgttcgcggt gcgcggaacc
    24721 tatgacgacg cgtttgattt gtgcatggaa gtttgccgtc agcgcggttg gttcaaccgg
    24781 aacaccgggc acaacccctt cacacgcgaa ggtaaaaaaa cggcggctat cgagctctat
    24841 gaacagctcg gacggatgcc cgattgggtg gtggtgccca cgggcgatgg caacatcatc
    24901 gccggcatct ggaagggatt tcaggatttg aaggaagccg gaattgtcgc gcgcacacct
    24961 aagatgctgt gcgcccagtc agaggccagc caggccatca gcgccgcagt atgggcgttg
    25021 caacagaccg gccaaaccaa gcccgattgg gccaaggtgc aggtggccgc agtacaggcc
    25081 acgacggtag ccgattcaat ctccgtggat atcccgcgcg atggccttgc cgcggtgcgc
    25141 gcggtggtgg aatccggcgg tgcggcggtc gccgtcccgg atgccgagat tctggccgcg
    25201 attccggaac tggcgcgtag cgcgggcgtg ttcgcggaac cggcggcggc ctgcgcttgg
    25261 gcggggctaa agaaggccgc gcgcgatggc ctggtacagc cggacgaaac cgtcgtttgc
    25321 atgcttacgg gcaacggcct gaaggatgtg gccagcgcgc gcaaagtcgc cggcgaaccc
    25381 gtcgcgctcg acaacaacat caacgccgcg ctcgccgcca ccgaaaatat ctgatgcccg
    25441 tgccgccaac tgcgccggta atcccttgaa gtttattaaa ttataggacc gaccccttag
    25501 ccaaaggggt cggtcctata aattaataaa aaagcacgct tatgttactg gatactgcgg
    25561 actgtgcgcc aagcgtcatg gccccagtcg ccttttctgg cttcttccaa tttcttgaga
    25621 aaagcggcaa agcaggtggc tgttcccatg cccagccgcg ctttgatcca ggtggttctc
    25681 atgctggtgt tgcgccggac cagccaggcg agggcaatct tctccggacc gttcatccgc
    25741 aacccggcca gctcggcttc cggccaaggg gtcggtccta taaattaata aaaaagcagc
    25801 gcttatgtta ctggatactg cggactgcgc gccaagcgtc atggccccag tcgccttttc
    25861 tggcttcttc caatttcttg agaaaagcgg caaagcaggt ggctgttccc atgcccagcc
    25921 gcgctttgat ccaggtggtt ctcatgctgg tgttgcgccg gaccagccag gcgagggcaa
    25981 tcttttccgg accgttcatc cgcaacccgg ccagctcggc ttccgtaatg cccaaggctt
    26041 tcaacccttt ggcaatccat tgctcggcgc aggcctcatc gtgtgcctgg acctccccgc
    26101 cactgaacga ctggcggcgg gcatttttca acgcaccccc gacccgctcc agcaactcct
    26161 gccggaactc ctccccgccc atgtgccatc cccgccggat cctttgccag gcttcatccg
    26221 ccttccaagg ctgggcacta tgacggactt cctccacccg tccagccata tatcccgcat
    26281 attttcgccg cccggctggc gtatcggcca ttcccagctc cgacaatact cgatgaaagt
    26341 tcaaccactc gggctgcgct gctttgccaa cataggccgg ataactgctc cacgcataat
    26401 caaccagccg ctgcttgtcg aaatcatatc cccggatgcg gaccggatta aggtggatgt
    26461 aggttgccag agtcgaaaaa tacccgctgc ccgcttccac cgggatcgcc ttgtaccgcc
    26521 cttggaacaa atgccctcgc tcacggtgcc ttgcattgaa ccgcttggtg taggtgctct
    26581 gcacccactg cattcccacc accagattcg gttccggcgt ttccagcagg agatgataat
    26641 ggttccccag cagcgtgtac gcatgcaccc gccatcccgt gcgctggcag acttcgccca
    26701 gcgttgtcaa gaacatcgcg cagtcagcat ccccccggaa tatcttctgc aaacggtttc
    26761 cccggctcat tacgtggtag accgcccccg catattccac tctgggtttt cttggcatgc
    26821 gccctttaca tcatttttac ccgtaccacg tcaattcatt ttattaattt ataggaccga
    26881 cccctttggt gggatggacg gggggcggaa gctgcggtag atttgtatgg atatgcatac
    26941 ccacttactg tccaaccggt tggctgttgt tgtttggcgt ggcgtgtttt gggccgtggt
    27001 gctgctggct ctgggcggct gttccaaaca ccgggctgga gacgcaccgc ggccggtgga
    27061 cccggacctg gcccatcctg tgttgaacta cgcggccatc gcacccggcg ttgagcatgc
    27121 ggaatacagc cgaactcagc ccgcgccact atcctaccat gttgtgcggg cggatctgac
    27181 ccaaggcggc ctgtccttgg cggttctgcg caccgccgcc agttccggtt ctgaacgtgg
    27241 ttcgctgctg gaaatggcac gggccatcga gacgccggag cggcgcaccg ttgcggcgat
    27301 tagcggcgat tattttggca atggcatggc ggggacgtgg ggcattctgc tcgtggatgg
    27361 ccgcttgcgg tactcgcctt ccggccgttc cgcgctgtta attgacgcca acgggcggcc
    27421 gatcattgac cgtccgcaat ccagaatcca gatgcaggtt ggcgatgatc cggcctggtt
    27481 tgagattcgc gacatgaacc gccccggcca aggggaggag ccgggactcc acctgtatgc
    27541 ccgttcgtct gaagtcaccc atgtgccggc cccaaagggc gccgtggcca ttgccgctga
    27601 tctgcccttg gccggcggtg acgtcgtggg aacggtcacg ggcacctttg ccgccgagga
    27661 ttcctttcgt ttgccggaga ccggtctggt gcttgccggc gccggcgatg caacaaagtt
    27721 gccggccggt ttgcgcgaag gcgcgagtgt ccgaatccgc accacaatta tgcccgccgc
    27781 ccgggaagcc gtcggcggcg ggccgcggat tgttcgtgac ggcaaggtca gcatcgaact
    27841 ggaacccgac ggcatttgcg ccgcggaggc ctcgtatttg aagcggcgtc acccccgcgc
    27901 cgtggtgggc ttggccggtg gcgggcaaac cgtactgatg gttcttgtcc ggggtcgctg
    27961 caacgaaagc gagggcataa agctcgatga tttggccgac ctgctggtcg gcttgggcgc
    28021 tgccgatgcg atcatgttcg atggcggcga ttccgccacc atcgtggaaa atgacagtta
    28081 tgtcatccaa ggtcgcgccg gggcacggca catgtgcgat ggactggcca tccaagcgcc
    28141 gcggacgcca gagcaggccg ttgccgggag cgcgccatga gcgtccgcag cttgagccgc
    28201 ttggcgctga ttttgggtgg ggcgctgctg gcgctctcct tcttcctgcc gctggggtac
    28261 cattcaccgg gcgaggagtt cctggccacg gtggattcgc tgacctctgc tgaaagtgcc
    28321 ggcgagttcg tggttgccgg cgggctgttg atcgtgatcg cctactccta catttgggcg
    28381 gtgctggtgg ctttggcggc gctgtggaac tttgcgggaa agcgccgcgc ctggacgccg
    28441 gttcatgtgg ccgtgcagtc catcggcggc ttgacgctga tgacgctgtg catcttgctg
    28501 ctgtcgttac acgatccttg gctgcccgcc aagttccagt ggaccggcgc cagcctgccg
    28561 ttggtgatgc tcgtgccgtt gtgggcggtc gcgttgctgg ctgcgccggt ccgccgcgaa
    28621 tgggtggtgg tggcggtcgg catgttcccg caagtgctgt tccagttcat gctggccggg
    28681 gtgagtctca gtcgcgaagg ccaggcccac ggcttcgtga tcggcggcat cggggctctg
    28741 ctggccttgg ccggcgccat tggccgtgcg cgaatgggcg cgtctcttat tggaaagtag
    28801 gggcggtggc gggggaccag cggacggtga gggggggggc ggcggggcgg gtgaattcga
    28861 gggcgtagtc gccggggggg gtatcgagac ggcgcccgaa ggtggcgccg tcgagttggg
    28921 ccagcatggt ttgcagcagg ttgaacgccg ggcgagggcg ccaagcggcg gggtcggtgt
    28981 cgtcaatcaa accgaagccg cgcgcggcga gtcgccacca atagacgcgg gacacgtggc
    29041 cggaggcgag ggtgaggagc agatagcggg ccatgaaggc ggcgtagtcg gcttcactga
    29101 cggagggatc gttttggcgt gggtcgcgcg tttcgtaggg ggagttgaca ggcgaccaca
    29161 cgccggtacc cagaaggggc cagttcactt cggaaatgag gatgcggtct tccgtgaggc
    29221 cgtaggcgcg ggcataggcg cggagcagcg cgcatttggc gacggtatcg aaggggccct
    29281 gcggattttc cggcgctccg cggcggtcca cgtagaggtg gtgcgagagg gcgtgggggc
    29341 gctgctcggc ggggatgccg cccaagagcg cgccgaggac gtagggctca aaatcaatgc
    29401 aggcggggcc ggtgattttg gcttccggat actgaatgga acaggcgtga acgggttgga
    29461 gcagttggcg atattcgcgg aaatcccaca cgccccactt ggcgcggttg atggcgtggc
    29521 ccacctcaaa aaagtcagcg taggaatggg cgcggtcgaa ggccatggac accatttgcc
    29581 gccaaccttt ggggtcgcgg atggcgcgac ggtcctgcac cagcgcgagg ctgacaaggt
    29641 gcccttggcg gtgcaaatgg cgcgcctgat cgagcgtcca ttgccaagcc gccgggccct
    29701 ggtggtgata gagccgcagc agcagcggca tgggatgccc accttgcagt tgcgccagcc
    29761 attccagttg gcggtcccaa gtgtcgggtt caggttcgag ggtcatgccg atggcgttgg
    29821 caaacggcac cgggcgggtg aaactttcct gccgcaaacg gcgatattcg cgctggatgg
    29881 gccagccttt aaccacggcg ctgtgcaggg cggccacgac attggcggcg ggcagcagtt
    29941 tgcggcggtc gcgcggcgta taggcgggga tggcttgcgc ggagcggtcg tcccaaatcc
    30001 aaatttctcg tccgcgcagg ggctggggtt gcgcggcttt ttcgcggcgg atgcgggctt
    30061 cgcggcaggg gtggcgcagc gggcgcaggg cggtatgccg ggcaaatgcc gcgcgcgtgc
    30121 gctgctcggc acgggcaaac tccgcggggg gggcgtagcc atgaaaatac atggcgtgga
    30181 acacgcggcg gaaatcggaa ggcaaatcca gccgcgcgag atcggcgccg cgttgggcgg
    30241 gggtggggtt ggccgcgtgc gccgcgcggt tgagatcaag aaacagcact tgccatggcc
    30301 gcgtggcgtt gggagtgggc cgcagagcaa tgttttggtt gccgagatcg cggtgcagga
    30361 tgccggcatc gtgcagggcg cgcacggcat cggcgacatc ttgcagcagg tccatcagcg
    30421 gggcgcacaa gggggcgtgg gcgtagatgc ggttcagttc gtcgcgcagg ttggtgaggt
    30481 ccggctcatg gcgggccacg aagcgacact cgcgcaggcg cgcgccttcc cagcgttcca
    30541 ccacggccaa cggcgccggg gtgcccacgc cggcctgttg caaggcgagg gcggtgcgga
    30601 acgcgcgctc ggccttgctg ccggcgcggc gggccagacg gtcgcgccag gccgcttgcg
    30661 gggcataggt tttgacgacg gcatccaccg cgccgccgcc aagcggcagg gtaaggcggt
    30721 ccactcggtg ccggccgggc gggacgaggg gggcgccctc tttttccacc aaatagggca
    30781 gttggtcgag ggtgggcagg ctgtccgggt ggtcccagcc gggagccacg aggaagcgcc
    30841 gggcgccgcg cgactgccaa acggggtcag tcatggtggc cgtggtcacc ttgaggctgg
    30901 tggtgcttga tccacagctc catcagcttg gcatatttga tgaacacgcc gaaggtggtc
    30961 atgaccgcga tgatcaaccc ctgccggccg tcaaggatac cgcgcttgat gaagagcgac
    31021 ttgaacaacc gccacggcgg gcggaaaacc aagtcgcgcc agcggaactt ttccccgcgg
    31081 gcgtattttt gggcggcgga aatcgtggaa aagcggttga gggtgttgag gtggtcggtg
    31141 acatcgtcgt aggtgaaatg gtgcaggggg aaacggaggg ttttgatcgg gccatccacc
    31201 acaacgcggt cgtggggttc ttcgccggcg ctatgcccgc ggtctttgag gaatagacgc
    31261 agcttgatgt cggggtacca ctcgccgtgg cggatccatt tgccgaggta atagaccatg
    31321 cgggggaact ggtaggccac aatctcgccg gggccgcgcg cgaattccgc ctcgatttcc
    31381 tggcgcaatt cgtcggagac gacttcatcg gcatccacga acagaatcca cgggtagcgg
    31441 gcgagggtgc ggatgtagtt tttctgggcg atatagcctt cccacgcgtg ctgatacacg
    31501 tgaggggtgt actgccggga aatctcgaaa gtgcggtcgg tgctgaagga atccaccacg
    31561 atgacttcat cgcaccaggt tagactggca aggcaggcgg cgattttatc ttcctcgttg
    31621 ccggccgtga cgcaggcgga gattttgaga tggtccgggc gcatggtggg gtcaatgtgc
    31681 cgcaggacgc gggttgagac cagcaaaaaa gctattcgcc gagcgccagc gccagcgcgc
    31741 gtagcgcgta gtgggtggcg cgggtggcgg cagaaatatc ggcggagtcg gtatccgtcc
    31801 agtaaccggt gccgtcagtc gcgattttct ggcgctggac caactggtgg aggagcgcct
    31861 cgcgccacgc gacgggttgg tcatccgtga tgaagacaat gcgttgaccg gcctcggcca
    31921 aggcggcggc cagtgcggcc agcgaggaat aaaggccggg gggggcgtcg gccagcggcg
    31981 ccacgaggag agggaggagg ggggcggggt ctgccgcggg ttcggcatcg gctgacgggg
    32041 gggcggggtt tgcggccagc cattgcgcgg cgcggtcgcg ggccgccagc gcttcgttgc
    32101 ggacagaaat ggccacgggc ccggcctccg gcgccggctg gatttgcggc ggcggcgcag
    32161 tcgcggcatc caatgcggcg agcaccgtgc gggcaatcag ctccgcacct tgcgcgctgg
    32221 ggtggacgcc atcgggcagc agagccaacc gattgggaaa gttggccgaa atatcaatca
    32281 cgcctgcgcc ggcttgcgcg gccacggtgc ggatggcagg attcatgacg ttgcggatgg
    32341 tgcggttggc tttgccctgc aggaacgcgg gggccaacgg ggtcagggtg cataaaaaca
    32401 ggcggggggg gatgggttgg gcctgccaga ggcgggtcag cgccaagagg tcgtcggcat
    32461 agctcgcggc atggtcaggg taggagagat cattgatgcc gagcatgatg accacaatat
    32521 ccggcgcaaa atcctgtgcg gcgcggcagg cgggggtgtc ggtccacgcg aggtaactgt
    32581 tgtggagggc ggtggcgccg ttcacgccaa agttgccggt gaggtagcgc tccggcgcca
    32641 gccgttggag cacgacggga taggcttgtg tgtcgcggtc ggtcagttgg tcgccgtagg
    32701 tgatgctatc gcccacgcag gccacgcgga gggggggggc ggcatgggcc aaggcaggca
    32761 tgagcaaagt ccaaaccgcc agccagcgcg ccttcatggc ccggggatgt cgcgttgggc
    32821 gcgcgcaccg aaacgcagcc aagcccgaat ggcgtaggaa atgaagaacg agacgaagcc
    32881 cgcgccgatg atggcgaagc tcaagttgcg gccatctccg gcggcgtctg cctgatgatg
    32941 cagatagagg aacacgccca ccagcgaggc cgcgtagccg agaatccggg tgatccagta
    33001 gagtcggatg atcatgcccg gcagtgtacg aggctgcccc gccgagggca aacggaaatg
    33061 cgttcgctta caccagattt cgttcgactt catgatgggc aaaaggtagg ttggaagtgt
    33121 ggaggcacgt gtctccaacc gaggatgcaa ctggatggat gcccatgaat agaacaagca
    33181 ggcgtgcaag gggtttcaca ttgattgagt tgctggtggt gctggccatc atcggggttt
    33241 tggccaccat cctgctgccg gccatcaatg gcgccatgaa gaagggcaag gtggcccgtt
    33301 gcaaaacgga agttgccgat ctggaaggcg ccatcaagcg gttttacgcg gaatacaaca
    33361 aaatgccgct gcccaagggg agcaagttcg gcgatgccga aaacggcttc accgatttgc
    33421 agcaggccca aatcattcag gtgctgctca atctggacga tggcggatgg cccggcggca
    33481 aacgcaacac caagcagatg ccgtttttgg atatcagccc ctccgcattc tggattgcca
    33541 gtcagaaccg ctacgccaag accattgtgg aaatggaagc ggcgctggcg agcgggcagc
    33601 cgtattgcga cccgtggaaa cagccttacg gcattttgat ggacttgaac atggatgacc
    33661 gcatcaccgg cagcaagttc gcgccggaaa tccgcgccaa ggtggccgtg tattccatcg
    33721 gcgaaaatgg tgacgacaac aacccgctct atcggacctg gtaagcgcgc gcgtgaggaa
    33781 gaggaaacaa cacatgaagg caggcagaca gcaacggcaa ggcggattca cgctgattga
    33841 aatgctggtg gtcattgcca tcatcggcat tctggcggcc attacggtgc cgggagttgg
    33901 caaggcccgc aaggtggcgt tgaagcgccg cgccatgatg gagatgaaca gcatcaagct
    33961 ggcggtgcac caattctatg acgaccaccg ctacatgccg tggggcgacc ccaacgcgcc
    34021 gaaccaggcg cgggtggggg acgatgtctg gaccgccagc ggcgccgacc aggcccgcgt
    34081 gattcaatgg ctgacgggcg acaaccccaa gaaaaaggcc tatctgcaag tgcctgacaa
    34141 atcgcggccg agcgcgtcat cgttccaatt caacgacccg tggggcgagt attaccggat
    34201 tgggatggac cgcaacatgg atggcgccat ccggccggat ggcggctccg gcggcggcga
    34261 ttatgtgcgc gatcaggtgt tggtctattc gctgggcgac ccggatgccc ggccccggac
    34321 cccgctcaaa acatggtgag cgcgtacgag agaccgatgg acagcgacag gatgaagagg
    34381 atgcaacgga tgaaagccca acaacccaga acccgaaccg ccgcccggca aggcttcacg
    34441 ctggtggaaa tcctggtggt catcaccatt ttggggattt tgatggccat gatggtgccc
    34501 gcggccggcc tgatcatcaa gcgcgccaaa gtttcgcagg ccaaatccga tgccggtgtt
    34561 gtggtggcca tcatgatgaa gtaccgcgcg gaatataacc ggtggccgtc ctttgccaaa
    34621 tcgcagggca ccttgcggac ggatggcgac tgggtcaagg ccatgagccc caagcccggc
    34681 ggcggttttg atgaaaacaa tcccaagcgc attgtctttt tccagccggg cgcgggagcc
    34741 ttggatgaaa ccaccggcgc gtttgtggac ccgtgggggc agcctttcgt ttatatgctg
    34801 gacattgacg gcgatggtca aatggacgac cccagcggca gtgcccagaa gatgggggca
    34861 tccatcttgg cgtggtccgc tggcccggac caggacgaaa ccacctttga agacaacccg
    34921 gtgagctgga aatgagtacg cgctgcaaca accggggatt cacactgatc gaactgctgg
    34981 tggtggtggc catcatcggc gtgctgtttg ccgtggcgct gccggtgttt gaaaatgccg
    35041 ggcgcaagga tacggaaaaa gccgcgttca atctcatcac caccatgcgc ctggcccggc
    35101 agcatgccat cagcaagcgc cagtgggtgc tggtggtgtt tcccaaccag gacggcggca
    35161 actatgacga gtccggaccc aacggcattg acaaatgcct gcgcgcttac gccgccatcg
    35221 ccgtcaccaa caacatggac gggttcgacc gccccgcgca gattccatcc aacatgaaat
    35281 atgcgttcat ttcggattgg aagtacctgc ccgaaggcac ttattttgac gacgatgaaa
    35341 cgctgaacgg caactatctg ttcaaggagc gcgggacgga tttccggtat ccgttggatc
    35401 cagcccggcc caactcgtcg ccgacgcgac ccatgggcgt ggtgatgttc cgccccaacg
    35461 gccgggccta caaatttgcc ggcaccaccg gcaccggcaa atattggcaa gacacggact
    35521 actccaagat ttatgtgact tccgccaagt attatgagcg gcaaaatgga cggctggttg
    35581 gtccgaatgc catccccggc ggcaccaaca ccgcggtgga aattcgcaac aagaccgggc
    35641 aggtccacat ttgggacggc aactagggga gcggtcatga agcacacagc caacccgaaa
    35701 tcaaccagcc gcgcgggcta ctcgctggtg gaagtgacgc tggccctgct ggtggtggcc
    35761 atcggactga ccgccacatt cgcgctgttt cccgaagggc tgcgcgcgac gcgctctgcg
    35821 gtggatgatg cggaaatcag cctttttgcg gactatgtct ttaccacgct ggacctcacc
    35881 gcggccaagt atggtggcaa ctacggcccc aattggacga ttgccaagac ggatgacttt
    35941 atctcgttga tgctctccac cctgccgtct gcacaggaca gttttgaact gaaagacggc
    36001 gggcgtaccg ccaacttcta ttggatgccg gattattacg ggctggatcc cggaaagttt
    36061 tccggcacgg attacaacgc caacagtttc cggagcgcca agttttggac ttcggccttc
    36121 acgtatacct tggaaattgt gctgggcact tggaagggcc acgggcaagg cgtgaccacg
    36181 tgcgccgtcc tcaaggtatg gcccggagaa tattccggca gcggcaaacc gaagggtgag
    36241 ccgcgcacgt tttatcgcga aatcatcccg attcggtaag cggagagcgt atgagaatga
    36301 agcaggcaac acacggcacc ccccgggcat ccgccgcgcg ggtcggccac cggcaggggt
    36361 tcaccctgat tgaagtcctg gccgccatgg ccgtgctggt cattttggtg ctggccctca
    36421 cgcggatgtt catggaggcc accagcatca cgcgccgcgg cacctctgct ttgttgcgca
    36481 acagcaccgc ggaaaccgcc atggaaactt tgctgcagga tgccgagggc atggtggtga
    36541 atgaccggct ggcctgctat gtgaaggccg ataccgccga tcccaacggt tttggctttg
    36601 acgatgtctg gttcatttcc acctccggcg accaggacga cgatttgccc tacgaatatt
    36661 tccactattt cgtgaccaac aagatttata cgaattcgtt gggcgcgccc tatgtccggt
    36721 tcgacctgat gaaagaacgc atgatttttg ccgtgggcga cgatatcaaa tatggctccg
    36781 gcacccgatt ttacgccatg gaaaccaaca ccgtgcagtg gtggacgcgc gcgcgcaaca
    36841 tgcccggtca ggtgtgggac cggcaggtgc tggcggacaa cgtcgtccgc tttgacgtct
    36901 attgcaccgg ctgggacggg caggcgtgga tgggcggttt ttccggcgtg catgagttcg
    36961 acagcacccg cgggcccaga ggcaacatcg ccctcgccgg cattccgccg gcggcattcg
    37021 atatctattt gcaagtcacc agtcccgaca ccgccatcga aagcggcatg gcgctgatgg
    37081 ccggcgtgcc caacgatgtc caaaccaagg cgcgggaaaa aatgattcgc gattcgtctt
    37141 cgctgttcgg gagggcggtg ccgatcatcg gcgcggcccg ttacgtcacc gtcaccacct
    37201 atggaaccaa taacacggtc tattatgaag attagttcgg atttgcacgt gcgctccggc
    37261 cggcccgccg caacccgccg caatcagcag ggcgtggcgc tgattgtggt gctgggcttc
    37321 ttgagcatca tgatcatcat ggccttggcg tttctgacgc aggcccgcgt ggagcggcaa
    37381 gtggccagcg ccacgctgga agccatgcgc ggccgccaaa tcctccgcac ggcgctcaat
    37441 gctgccatga atgattacag cgttgaactg gcggcgcatc agttggtcat gcccccggtc
    37501 aatacggact acgacatgtt cacttccatc ccgcccaaac ccatcatggg catgggcggg
    37561 cgcaccatcg gccaggataa tatcgaattg ctggtgggcg aagtggaaga ctggatcccg
    37621 cgcaagtact tcaacccgcc atacaatgcc aaaaccaggg tgccggccaa cgccgaatgg
    37681 attttggtgc gcgatgatcc gtcatcgtcg caacccagtc ggattctggg ccgctatgcc
    37741 tatgtatgtt tcgacatgag cggcggtgtg gatggcaatc tggtcgcgcg ggaaagcgaa
    37801 gtggcggggc aggatgcccg ggtggcatcc aaccgtattc gccgcagcgt gcggcagatg
    37861 cccatggggc tgctgccgga ggccgtcaac ccgtcgcagt tcaagagcta ccgccgaggg
    37921 tggaaaggtt ttgacagcct gcaaacgctg atcaagctga ccgatgggaa ggcacaagat
    37981 ggcaacgatg gccacacccg ctggcagccg gagcgcaaag agattttcgg tgccggtctg
    38041 gcctccaact tggtgagtga tgtcgtgccg ttcagtttgt cggtgtaccg gggcgggcgt
    38101 tatagccgtt ccagcgccac atggtccaag ccggagtttt gcggtgcggc gccatggacc
    38161 accgtgctga gccccatcca aagccagttt gcgccggggt ggcaggcgtg ggtgtccaat
    38221 gccatcatgg atttcaccag cagcagtcca tatcccatcg gcacggatta tccctcgccg
    38281 aaaaatgtgc cgatgttcaa cgaaatcaac atgtcgtacc ggctgctgac accggagcag
    38341 gacccggtat cgggctggta caaatacacg gtggaagtca cgttgacggt tgagttctgg
    38401 tatccgttcc cgtccgaaga caacgtgcgc agcggcacct acaacatgcc gccgccgacg
    38461 ctgacgggcg gcgcggcggc cggcgatggg cagatctgga tgcagatggt catgcccacc
    38521 gtcgccccgc cgctggtggc ttttgatccc agcgccgctc cgccggccgc gttgagcgtg
    38581 gaggccaaat acaacaatgg tcggccttat ctggccaccg gcaccaccaa ttttgtttat
    38641 aaaattccat tgatccggcc ggtaccgccc agccccgcgc cgccggacta caatccggct
    38701 tatcccttcc cgaacaacgt gtcgccactg ctggtcaaca tggtgcaggt ccgcaagccg
    38761 atttatctgc gcgccggtac caacaacgtg gacatgattc cgccgggact gaccattcag
    38821 ccagtcgccg ccaacgtgat gcccggcgcg cccgtcacca aggtgtatgt gcaggaagtg
    38881 acggatccgc gtttgaacca catccccggc caatgggtgc tgcaaggtgc gccgtcgctg
    38941 gaagcccgga acacgtgggg cggttccacg ctcaacaaat tcatggccga gggcaccaat
    39001 ctgtattgcc gcaacgcacc gatggaaacc cccgccgatt tgggctacat cagcaccggc
    39061 aaggaatggg agaccattga tatctgtacg cctgcggcag cagatgttct cgccaacctg
    39121 atcgcggatc ccaatctcta cgcaaccttc tccagcaaca aggtgttcta taccaatggc
    39181 accatcaacc ccaacacgcg cagttccaac gtgctggcct gcgcatttta tgatttggcc
    39241 acccaggaaa cgcccaacac caagaccgag gtggttggcg ccaatccgat tgacgaagcc
    39301 acggcccgcc tgctggcgca gagcatcatg gatagcacgg tgtccggcac cattgataac
    39361 gcctgccaag ccggcagcga ctgggtccgc tgtgcggcca tgcagcaggg cggcgcgctg
    39421 gcggcgattc cgttgaacaa caaccagcgc gagtccctgg ttcgcaacac ttggggatta
    39481 ttcagccccg acaacagcct gttcaccgtg gtggtcgtgg cgcaaaccat caaggaaggg
    39541 cccaacaacg tcggcacgtg gaatgcggcg gatgacgtca tcaccggcga acgccgcgcg
    39601 gtcgcgctgg tctggcgcga cccgtataaa aacggtgtga acccgcacca cgaaatgctg
    39661 gtgcgcatgt tccgctacct gaacgattaa ccccccgctc gcgccgccgc gcggcggttg
    39721 gttcgtgggg tctgtttctt tcggcgtttg gggtggagga tttcttgtgg attttggcgg
    39781 cgccaaaacc aaccggcatc ttttccgccc tggcgcgccg ccgccgcaaa gcggcggcag
    39841 aggccagagg tcagagttca gaggtttttc tgtggccggc ctcggtgagg ccggggctgc
    39901 tggttcggcg gtttttacct gaaatctgaa cccttttttt ctgctgggtc cgatggtttt
    39961 ccacactgtg gaaaaatcgg cagcaaaagt tccacaccgt ggaaaagttt tttccacact
    40021 gtggaaaaat caggcccaaa agttccacgc tgtggaaaaa cggggccaaa aagttccaca
    40081 caatggaaaa tagacgagtg ccgagcccaa gtccgcagga cgccgggcga acgcgagttc
    40141 tatagcggcc ggcacagccg gacgagcctg cgagccatag cgagcgcagc ggcaaagttt
    40201 gccggatttt ccacgcaatg gaacaacttt gaggaaagtg tatcccgcgc gggcggggtt
    40261 ttccacactg tggaaaaatg aggcggatgg atcttgcggc tactgaaccc tgaaccctga
    40321 accctgacct ctgacctctg ccgccgagcg tatcgcgagg cggcggtgcg gcgatgcggg
    40381 tttatgggcc gtgggggtcc acgcggtaga aggcgcgcgg cttcacgttg gtgctccact
    40441 tgacgttgaa catgctctgg ctggcggcgt tcaaggtgtg cgtgaagttg gtgctggtcc
    40501 atgagttgat gggcatgagc aggttggtcg tgaagcgcac atagaagttg gtgaccgggt
    40561 caaagaagtc gttgccttcg gtttgcagaa ttacgttggt tgtgcccagg accatgttgg
    40621 tgatgaaaat gcggtccgca atcgggaatt ggtggtggaa cggatggagt tcggcgccgg
    40681 cccagttggt gtacacggtg ctgaggttgt cgttgccgtc gtcggagccg aggaagacgg
    40741 gttgtacgcc ggccttgttg ggatagacgg caatgaagta ttccatcgtg tccagacggc
    40801 ggtaggtgcg gggttgaatc tgcgcataca catagctgta gccgtcggca tcctgcgagc
    40861 cttcgcggag ggacatggcg acctgagtcc acgggttgcc gctgtcaccc aagcggtgat
    40921 agagcgtgcc ggtggggctg agataggcat tgttggtgta gccgtaatag acgccgacga
    40981 tgtcccagtt ggcggcgttg agcgggtcca gcatgtaaaa cgccgcggcc acgttggtgt
    41041 tgagcggggt cactttggtt tgctggtcgt gccaggcata ggcccacaca ttggtgggct
    41101 ggggctcttg cagcacttgg ccgtcgttgg gttcgccgat ggtcaggtcg cccaattccc
    41161 agccgaaatc tgggtaggcg gagccggtgc caatcaggcc gacggagttg gtgtcttgcg
    41221 gcgtctggct ttgcgggatg cggtccgccc cgctggcata gcccacgtag gacagggagt
    41281 agatcaggtt gccatacaaa tccaccagcc ggatcacgcc cacgccatcg tggatgtggt
    41341 ctttgtattc caccgcaaac ggctgcacgc taagcggcac cagcgtggtc aggttttgat
    41401 taaccggatt gcttccggcc aattcggcat cgccaatcac atagaacccg aatccgttgt
    41461 tctggttgct caagatggtg ccgttgggaa tggtgtaggt ggcgtagatg ggctgattac
    41521 tgttggcggc aatgtccaaa tccgcgccca gcgccagttg caatttccag ccgctcaagt
    41581 cggagccggc cagcccgcag atttccacga actcatggtt ttgaccggtc caagcaaaat
    41641 aaggatcttc atccaaaaac acaaacggtt cattggtgga ataggaggcg tagcccaact
    41701 cattgatcca gactgagccc tgctgcacgg cggagatgaa gttggtccgc gtggcggtgg
    41761 tcagttggtt ggtggcatag cccggcaagt tggtcacggc gccaatgccg gcgtattgca
    41821 cagtcacggt gtacatgatg cgggtgccgg cggattggcc cggtatcgtg ctggtggcga
    41881 cgtagttgcc gaagtgttcg ccgggcacca tcggcacggt gttccacgcg ccattggcga
    41941 tgcggtagcg cccggtcacc gacacaatgg atgcgccgta gcgggtgacg atatccgcgg
    42001 ccaacgtcag gggggtgtcc accagcggcg cgggcgacaa accgggcacc accatgactt
    42061 ccggtcgcgg ttggggcgtg ccgatgcgga tgtcatccac cagcacgcgg gaggcgccgg
    42121 cgctgtggta caggcgcacg tagtggttgc tggtgtcgta gaggaacagc gtggtggttt
    42181 cgtaggtgat ggattgaatg ttgatcgtgg ccagcgtggt ccatgtggtg ccgtcgggcg
    42241 atacctgcat ctgcagggtg gggttcgtat cgctggcggc ccacttgcgg gtgcggaaac
    42301 tgaccgggcc aaggccatcg cgcaggtagg ggctgacgat gtagttgccc accacgctgt
    42361 tgaggcgggc cacctggccg acatccgcgt tttgcgagtc cacaatgcag tcggtgattg
    42421 cccagccttg gaagagcgac acgccggtgc cgtattggct tttcacgggc cagccatcga
    42481 agttctggtt gcggtaaatc atcggcagcc ggatttcgat gctgtccaca atggcatcgg
    42541 caaacggctt ggacggatcg ctgatgatgc gcgcctgcgc gttgggtgca tccatgaaca
    42601 gccaagcgga aaaggtggtg cccgtggtgg cggtgaacga agcctgcgtg gtccagttgg
    42661 agcccgcgtt ggtggtggtt tgcaagacca gataggcagt ctcggagaag tccgccgcgc
    42721 gcgcccagaa gcgggtctcg cccaggccgc catcaaagga cggcgacacc acgcaactgt
    42781 tgctggcgcc cagcagcgcg gcgcgggttt gcgagaggcc gttggtggtg atgctggcat
    42841 tttggatggc ccagccatag cgcgcgtagt tgccgggaat ggtgtaggac gggtccaccc
    42901 agcccaggaa atcttccgtg cggcgcgtgc cggtgagttc gctgatgacc acttcgtcaa
    42961 tgcggaaccg gttggtggcg ccgcccgttt gccggatgcg cagataggtg gcatcggccc
    43021 ggttgatggc ggccacataa taggcaaagt tgctggcggc cacgctcgcc aacgtggcgt
    43081 tggtgatcca cgggccggat tcagatgacg cggtttcaat gaccatggtg acggccgggc
    43141 tcatggattc agtgcgccac cagaagctga catcgcccac gccgccggag agttcgccac
    43201 tcatcagcgt ggcgttggtg ttcatgatca ggctatagcc ggcgggggtg tagggctggt
    43261 tggggtcaat gtaggaaatg tcgtgcgtgc ccaagccggt gaaatcgtcc ttgccggaaa
    43321 tgatccactc ttccaccagc gggaaggcgg cagtgacgct gctgacggta ccggtgaaga
    43381 tcgtgccctt gcgggtgagg gtgtggtctt gggtgttgcg gctccatgcc gagccggtgg
    43441 cgttggtgcc cacctgccca acgcgatcct tgaccgtgcc ggacgctccg cccttgcgca
    43501 gcacgatcac atcatcgccg ttgaacgtga gatacttgtt ggtttgcagg cgcagttggc
    43561 cggtgatggc gtcatccggc ttgtactccg caggggtgga gggacggccc accaccaggg
    43621 tggtgcccgg ctgcaacacg ccggagagcg cgatggtgac agtggccgtt gtggagccgt
    43681 tgtcgtactg ctgcagcacg taattttgcg cggccaagtc caggttgctc aaggtgccgt
    43741 tgtaaatctc gatggccttg ttgttgccgg tgccttcgat gtattgcgaa atgaacacat
    43801 ccgccgccgg gcgaccgggc tgcacgttat ataccgtttt gccgctggtg atggaccaat
    43861 ccggggcaaa ggcgccgtag gatgccaacg agccccacga atcaaagttc tgcgaacgcg
    43921 aagccaccac gcggaactcc gcatcatcca cctgcatggt ggcgccgctg ttggtggtgg
    43981 cgccggagca gaggaacacc acgtgcgcct tggtggccgt attcggcgcg gcgccttcca
    44041 cggagtattt gacccaattc gtcgtcaaac tgctgatgtc acgaatgaca tccggcccgg
    44101 cgatgccgtt ggtgacgtcc agccactcaa ttttgatttg catggtgctg gcggaccagt
    44161 tgggcgtggc cttgagccag gccgacgcgc tgtaggtttt gccggacgtc accgccacgt
    44221 cttgggcgaa ggagccgtaa tcggtccact cttcgaacca tttgccatgc acggccccgc
    44281 accagttgcc ggcgtgcgga ccaaacccat cggcgacccg cttgggccac gcctgccacg
    44341 aaccccagtc cacggcgtcg ccgccgtcgg gctgcgtggt cagttcaaag ccggggtttt
    44401 tcaacaggtt gatgccggag ttgtcggcat acatccggcg gacggagaac gcgccggaaa
    44461 cgtggtcaaa cgtgatgccg tagcgccccg gcgtgacggc gttgatggtg gcataattgg
    44521 tcaaggcgtt ttgcatggtg ccgctgaccg gcagcgtgaa gttcggcgtg ccgttggtca
    44581 cgccccagat ggccgcgccg ttgttggccg caaagcgcag cgtgaccggg ccgttattgg
    44641 tgatgtggta atccgccgtc cactggctgc tgttgcccac gcgcatcatg ttggccggcg
    44701 gcgcggccat gctgataaag ttgccctgcg ccgccatggt ggtcaccacc acttcctgcg
    44761 tggccgtttg cacaatactg aaactggcgt tgctgacgtt gagcgtgaag aagaaagtgc
    44821 ccggtgcaaa gccggcgagc gtgaacgatg atttgccgcc ggcgttgccg ttggtgatgg
    44881 gccaggccaa ggtttgcgct tccgtaccgc cccactggat gttggggttt tcgtccggct
    44941 ggaactggaa tgcggtggat gattccaagg tcagcgagcc gctccagaga tagtcctgcg
    45001 gcgcggggtg gttggtcagt tggctgttga tgttgggctg ccagttgttg aagtcgccca
    45061 ccactgccat gttggcgaaa gccggcaggg gcagtggcga agctcccgcc cattccatgc
    45121 ggaactccag cgtggagtcg ttgaacgtga accggtacaa ccccggagtc agatcggtga
    45181 acgacaggtt gccgccgccg ggaaccggcg cgcggcccac ggccggcacg cgggcgaccg
    45241 ccgccgctcc accccagttg gtggtccaag tgccgttggc cgtgaacttg aattcgccgc
    45301 tgccggaggt gactgtctgc gtggtgaccc atgtattatt tgccaccaac gtcatgttgg
    45361 gggtcgaaga ccacacattg gtgaatgccg agcccgccac ggccatggag gaatatggcg
    45421 aggaggccgc gccggccatg cccgcagccc ccaacaatgc cattgcgatg attccggctt
    45481 ttttcctcat ggttacctca atccggggtg gggcatatca tgttttaccc ctataaacaa
    45541 tacttcgttt ggtatctgtt agcaagaagc gcgcgtcgct tttcggcgtc cgggtttccc
    45601 ttccccggca ttggtcggcg gcgtccgggc agccggcgcc gcgccacatt tctattggcc
    45661 gaagcgtgcg ggaaacagta gattcaccgt ggcagtgaat gcgcgaggag tgcaaccatg
    45721 aagaaaacgg cattggcaag cttgtggatt ttggtggggc tggcggtgac cgcctggggc
    45781 caggccacgc tgccgacctt ccattccggt ccgtggtacg ggcagccact gccaaccggc
    45841 tggacgcaaa gcggcttggg caccgactac aaaaccgatt atgacaaaga tggaggcttt
    45901 gcggccaaat ttgacggttc gggcgactgg ctgcaaatcc gctacaattc cgcggcctca
    45961 acggtgtcct attatgccat gggcaatggc accagcggcg agtacgttta caaggttcag
    46021 gagtcggtga atggggcctc gtggacggat gtggtcacct tcaactccgg gaaccctctg
    46081 ccgaccagcg ccccggccca atacacgaac agcctcaacg ctgcctcccg ttatgtgcgc
    46141 tttatctatg tcacgaaggc caccggcaac gtggggttgg atggcgtgct ggtttccggg
    46201 ccctccctgc cgaccatcac cttcaccccc aatggcacga acagcgcgcc ggtcagcaat
    46261 cagttctcga tggcggtagc catcacgccg ggcggcgcgg ggctcaaaag ctggagcatg
    46321 gcccccgcgt atgctggccc cgcaacattg accaacggca cgttcacgtt cacgccggcc
    46381 gcgacggaca acggcaaaac cttcaccgtg tcggtggtgg cctccaacgt ggtgggcacc
    46441 atcaccaaca cggccaacat cacggtgtct gcctatgtgc cgcccgtgcc ggtgattcaa
    46501 ttttcccccg ccgcgccgta cagcgtgatg gccacgcaga cgcaaaaact cggcattgcc
    46561 gtatcgccgt tgggcagcgg tatcgccagt tggacgttgt tgccggcgta ttccggcacg
    46621 gccacgctga ccggcaccaa cttctccttt atcccggcaa cgaacgatgc tcccgccaac
    46681 tataccctca ccgtactggc caccaacacc ttcggcacca ccaccggtac cgccacgata
    46741 gccgtggccg cctacgtgcc cccgccgccg ccggctgcct atatctgcac tttcgaggat
    46801 ggcagtaaaa ccggctacgc ctccggcgat gtcaccctca gcaacaaagt gtggaacctc
    46861 accggcatct tgattggttc cgatgccgcc gatctgaaaa tcggcaacaa agccgcccgc
    46921 ctcaaatatg acccgacgga tggcgatgac gtcatgacca tccaaagtcc ggtgatgtcc
    46981 aacggcatat ccacgatttc attgtggtat gggccatacg gcaaccacgg cgacaccgcg
    47041 ccgacgctgg ccattgaagt cagcgaaaac ctgtccagcg gctggatcga ggtggggcgg
    47101 gtgaatgccg gcgcagtgga agtcctcacc tattattccg ctgatgtgtt catcaacacc
    47161 cccctctacg tccgcattcg tgcggttgat ggcaacggcg gccgcagttg caattttgac
    47221 aacatcacca tcaccccctt cgccatccag ccggtcagtc cctacgacgc attcctgctg
    47281 aaatacaacg taacgccggg ggatcccgga acattgccgg gcgatgacta cgacggcgat
    47341 ggttttacca accagcagga attcgacgcc aacaccaatc cgtacgacga cgcggtgcac
    47401 ccataacgct gttcgttacg gtgcgggagc cggcttgagt tccaccttgg gacgcccaac
    47461 aattgcgctg aatttgcgag gccttttgta gggcaggaat ggttgaggga tgaaactttc
    47521 ccgccgggaa cggctggaag gtctgcgacc
//
LOCUS       DAOPNP010000006        46176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1024_length_46176_cov_9.708332, whole genome shotgun sequence.
ACCESSION   DAOPNP010000006 DAOPNP010000000
VERSION     DAOPNP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 46176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46176
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1024_length_46176_cov_9.708332"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1092
                     /locus_tag="PKI64_01245"
     CDS             256..1092
                     /locus_tag="PKI64_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOD99663.1"
                     /translation="MQPVINLLVQLQELIIARAQHEAGQPGAQLDQLDASIASLKKEI
                     PADVRQRFERQLQKNSLAIVPTENGVCTACGMTLPVALVHQVHAAEQIYGCPSCARLL
                     FYRSGSARNTRKPPRRSDPPKVGIERFSSETLMIPALKGTDRESVLAELCQKLQDEGF
                     VDNAQQLQEAALQREAIVSTAIEGGVAFPHVRCVEGGALTLALGIAPKGIKFDPNGKA
                     NTRVVFFMVIPTAASAFYLKLLSGLAQVFQKPENRDKLLEADTAEKLWKALVKATRTV
                     IQ"
     gene            1113..1628
                     /locus_tag="PKI64_01250"
     CDS             1113..1628
                     /locus_tag="PKI64_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOD99664.1"
                     /translation="MIPRRNLVLLGFMGTGKSTLGRRVAALTGAPFLEMDAELERRAG
                     KPIPRIFAEDGEAAFRDMETALAREWGQQESAIISCGGGVVLRAENLTALRANGVLVC
                     LTARPEVIVARTARAGNRPLLAGDDPAAKVRELLAARAAHYAQIPLQLETSDADLSDL
                     AQTLLDLWLRA"
     gene            1632..2921
                     /locus_tag="PKI64_01255"
     CDS             1632..2921
                     /locus_tag="PKI64_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOD99665.1"
                     /translation="MWRYLARRLAQAVPTALGVVLVTFVLFNVVGGSPAAQVLGQNAG
                     PLALEEFDLARGYAKPLFCGRWTATRALAAVDFATAAPSVRAQWHAGDSGWLSLADGE
                     ELVLPLAFPLPAEGTWRLTVEGRGPGFPQCGKKFSTVWKKSAKSSTVWKKVFHGVEKP
                     TFRAAGGAVEIRRVRLERGTRHFFDSQLTDYIGRVARLDLGTSASLNQPVTRLLRAGI
                     GPSLALTVPILLVELVVSLTLALLCAYRRGAWLDRLLVAVCVALMSVNYLVWIVAGQF
                     LLAYRWGWFPVWGFESWRYLLLPVLIGVATGLGVNVRFYRTILLEEMHKDYVRTAFAK
                     GLRTERVLFKHVLKNALGPVITNVAVALPFLYTGSVLLENFFGIPGLGYLSLNAIHSA
                     DVDVVRAVVLVGALLFTAANILADVALAWVDPRVKLA"
     gene            2918..3823
                     /locus_tag="PKI64_01260"
     CDS             2918..3823
                     /locus_tag="PKI64_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99666.1"
                     /translation="MNNAAEQMGAGASFGREAWRRVWASGIARACLAVLALFFVVALA
                     GEAANAWYEWRDVTPPWQAQELAARYLPPGAGHWLGTDGLGRDVALRLAQGARIAFEV
                     GVVSSLLAVMIGVLLGCLGGYFGGWVDDLVVWLATTFAAIPGLLFVLAIAMVVGKGLL
                     GVFLAIGLTTWVGVCRLIRAEVLRQKERGYVRAAQALGFSSWRILFRHILPNVLHVAL
                     VSFTLRFPAAIGTEVFLSFLGIGAQGQPSWGTMIASARLRLWQGMWWELAAVTVAIFA
                     VVLAFNLLGDILRDALDPRLRVATD"
     gene            4096..5634
                     /locus_tag="PKI64_01265"
     CDS             4096..5634
                     /locus_tag="PKI64_01265"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOD99667.1"
                     /translation="MKKSDHVIVFDTTLRDGEQCPGASMGLHEKLQVARQLEKLNVDI
                     IEAGFPAASPGDFEAVRQVAATIKKCRIAGLARCVKSDIEKCAEALKPAGDRARIHVF
                     LATSAIHREFKLKKAKDEIIRQAVEGVTYARQFCKDVQFSPEDASRTEPEFLAEVVRA
                     VIAAGASTINIPDTVGFTVPNEFRKLLTYLNEHVPELQNVVVHVHCHNDLGLAVANSL
                     AAIECGARGVECTINGIGERAGNCSLEELVMALKVRNDHYGIQTRVRTQEIFATSRLV
                     SQLTGLMVQRNKAIVGGNAFAHESGIHQDGMLKHRETYEIMRPEDVGVVTGTDLVLGK
                     HSGRAGFSAHLKRMGIQLKAEQLQGVYDAFIALADKKKVVYDDDIIELLREYVSEIPR
                     VYAIKCLQVTTGNRALPTATVQIEKDGKIIEDSATGAGPVEAALKVIERVTGIKGELK
                     SFAMQAVTHGEDALGEAAVQVKFGDDLVAAKGSDIDIIVASAKAYINAVNRHLFLTQT
                     SAKL"
     gene            6016..7140
                     /locus_tag="PKI64_01270"
     CDS             6016..7140
                     /locus_tag="PKI64_01270"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD99668.1"
                     /translation="MPSTPGHLIRLLVWLLLALVGAGLAVWSWPGEGDQPVYPGRHWS
                     WMAPERAGYSAAKLNAFSRHTGGSGCIAYQGRMIYAWGDWAARGDVASVAKPLYAFLA
                     YKAVEQGRIGSLDDLVADWVPDNTALDEYLKKIAGPITFRQLLDQTSGYGLEESPGEA
                     FAYNDYATYLCAWLLFDRVWEYPEADNDAVLNGELLGGSIGFEDQPTIQHRQSRPFRL
                     RISSRDLARFGLLYLRGGRWRKATLLRADLFASHMHSALPADFPRTSGEEDELLPEAR
                     TVGGDKDEKHHLGSLGQFWWFNRVTPDGHRLLPDAPEDTFFGSGYGGRFALLVIPSQE
                     LVVAWQDIYPDEDWIPLDETGRHRLNQTIRALLAARVPAE"
     gene            complement(7150..7398)
                     /locus_tag="PKI64_01275"
     CDS             complement(7150..7398)
                     /locus_tag="PKI64_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOD99669.1"
                     /translation="MSLKHVTVRYFALVGARRGTQTEPYETTAETTRQLLHEIQQTGR
                     FPLTTNIVKAAINDEFVDWDAPIHDGDQVTFLPPFSGG"
     gene            complement(7395..9197)
                     /locus_tag="PKI64_01280"
     CDS             complement(7395..9197)
                     /locus_tag="PKI64_01280"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOD99670.1"
                     /translation="MKTNNQRLLKWVAEVAELCQPDQIYWCDGSAEENERLCAQAEAA
                     GTFIRLDPAKRPNSFAVFSDPADVARVEDRTFVCTTDPREAGPNNNWRDPVEMKDTLR
                     KLYAGCMRGRTLYVIPFSMGPIGSPIAHIGVELTDSIYVVVNMRIMTRIGAEVLAALG
                     DDGDFVECLHSVGAPLAPGQKDVPWPCNANNKYISHFPEDPSIWSFGSGYGGNALLGK
                     KCFALRIASTMARRQGWMAEHMLILGLTNPAGKKIYVAAAFPSACGKTNLAMLQPTLP
                     GWKAECVGDDICWMKFDANGQLRAINPEAGFFGVAPGTSMKSNPNAMRTLYANSIFTN
                     CALTPDGDVWWEDMTDTPPPKLTSWKREEWTPQSGSKAANPNARYTTPARQCPVIDPA
                     WEDPAGVPISAILFGGRRPSTVPLCFEAPTWQYGTFLGSIQASETTAAQAGSVGVLRY
                     DPMAMLPFCGYNMGDYFAHWLSMPGRVPNAKMPRVFYVNWFRKSDDGRWLWPGFGENS
                     RVLKWIFERVEGRGQAMESPLGLLPAPGAIDTTGLDIAADDMAELLEVDVAGWLKDIP
                     AIRAHYAKFGDAIPAALQQELNDLEARLKAAQTA"
     gene            9396..11555
                     /locus_tag="PKI64_01285"
     CDS             9396..11555
                     /locus_tag="PKI64_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOD99671.1"
                     /translation="MSEMNNAGDLENQLLELLTQDSAPAAAWLAVLTAGVTAGIPAAT
                     REWAAMAQEALAKAGDVAGGSDLLKWRADNTPPDQMTAKDWLKVADVLAGSDPYLLAL
                     IQEAGFGQKLAARECVRRLRLLLSLQPGALCLHRTWGFGVVQKADALYRKIEINFRGR
                     PGHGLAMKVAAESLEVLAETHLLAQWHRDRERIRALVRDNPAEVVRLALASFGPMPVA
                     TLQKTLVSAELLPDSDWKRFWDAARKVLKADPLVMVPAKRTEPLCLLNSAAGFDDTWF
                     DRLANERELKTIVERLGELAERAPAHDTIPAAQRQVMTNRLAFVLHSATSRQPGIKLQ
                     AALLAARLAVPPAECDWPAAAREFLRGPALVEVLHDLPARKVAPALEFLWAQDPEALR
                     AALLEQLGNLYFGPLQEVMVLLRKQGSDEACRDIFAATCAAHTIRQEMLLWLLRNVSL
                     IAEWDLPLPAALAPIVLAELEQDYKGERLKTRKQLREAFEDAAWLKAVFDGMTPSRQQ
                     DFFRRLNQSPAWPGLDRQVLQAKILKQFPQLHGVIMGAAAETPTASSGQVTSHRSYRE
                     RQEKLERLLNVEIPANSKEIAIARSYGDLSENFEYKAAKDMQRVLMARQADLEGMLAK
                     VRPTDFAEAPTDRAGVGTTVTLAYPDGHREFFHILGEWDQFPEKHIISSNTRLAQALA
                     GKQAGEDVEVPAEDGPPVTCRVETVTPLPDDIKEWVK"
     gene            11557..12057
                     /locus_tag="PKI64_01290"
     CDS             11557..12057
                     /locus_tag="PKI64_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOD99672.1"
                     /translation="MTLRERWQHYLSHDAPPLVQFVKYGLAGGIATVTHVGSFFLAGF
                     LLFPCVGADDPLVRLFNLAAPVVNEALRARYAVYSNLIAFGFSNTVCYLINRWFVFRP
                     GRHHVVLEFLLFLGVSAISMTVGTTLMGLLIKYLGMPTTYAFGANILSSLAINYVLRK
                     YFVFKG"
     gene            complement(12325..12876)
                     /locus_tag="PKI64_01295"
     CDS             complement(12325..12876)
                     /locus_tag="PKI64_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD99673.1"
                     /translation="MTRRWMSVLMVLLAVAMVGTTGCMKKKKKPLGTDAPYSDQVVIG
                     YDANGNPIYGTAADLAGLAARTEGAEVAGQFEAVLFEYDSTQLNPSEQAKINAVVGYL
                     QQNPTAGIVVEGHCDERGSNEYNLSLGERRALAVRAAMIAAGVDGARIQTRSYGEERP
                     VAFGHDEASWRLNRRAEFLSTQM"
     gene            complement(12930..14138)
                     /locus_tag="PKI64_01300"
     CDS             complement(12930..14138)
                     /locus_tag="PKI64_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOD99674.1"
                     /translation="MKKQSWLVVALLCGVLTAWGQSSHINVVKAAGGKVGVDLSGIQV
                     SGAAAQTARRTLELDIARSGWLTVAPKGGGSVIVSGTLTEAGGAVVFTCQAVNPAGQA
                     YLNATYTQPANQAIYLAHQVAHDLLGKVMNKPSFFLAKLVMVGTRSGAKELYLADSSL
                     QDIRQVTQDKSVAIKPRWSPDNRFISYTSFLKRYPDVYTIEMATGNRTRAASFPGVNS
                     GGAISPNGRSMALILSKDGNPDLYVMDIASKRLTRLTNTPRADEGSPAWSPDGSRIVY
                     VSNASGSPQLYIISASGGAPQRLTRSGSQNVAPDWGQNGLIAYQTQSGGKFQIAVVDP
                     NIMQERVITPFDASYEDPSWAPDGRHLVAVRGVNYSYSLYLLDYMAEMGDKPIALTTT
                     GDWTGPAWSN"
     gene            complement(14251..16818)
                     /gene="mutS"
                     /locus_tag="PKI64_01305"
     CDS             complement(14251..16818)
                     /gene="mutS"
                     /locus_tag="PKI64_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOD99675.1"
                     /translation="MFWLFSRATVCFIVATTMSEKSTTPMMAQYRRAKAEIAPDTILF
                     FRLGDFYEMFFEDAIVAAEILGIALTKRQGVPMCGVPYHAADAYLAKLIRAGKKVALC
                     DQMEDPALAKGIVKREVTGIVTPGTVLTDSMLDAARPNYLAGLHRQDKLYGLAMLDLS
                     TGEFWMEEAADPTALLDDLARYAPPEVILSERLHADEAFLSALRATGDFALTARDDWI
                     FDPATADDGLCRHFGVQSLAGFGGEGHPAATAAAGALLYYVTRDLRRNAAHVRRIRFR
                     TATDAMMLDESTLANLDLLVARGTPRADAPTTLLKALDTTRTAMGSRRLRDWLVRPLQ
                     QVPAIVARHDAVAALLKTRHALDALRQTLGAIKDLERLLARLSSGTGNGRDVRALAAS
                     LEKLPALHDALAAYPGGRLAELRGQIVPQDELVALVARALVDEPPMSIKEGGVIRKGY
                     HAELDELRAAAHDGKQWIAELQAREIERTGITSLKIRFNKVFGYFIEVTKANLSRVPE
                     DYIRKQTIVSGERFITPELKDYEHKILGAEDKSRALEYELFCALREEIVAATAAIQQT
                     AAAVAEVDVLAAFAERALTCRYVRPQISAAGPLVIREGRHPVIELLPESGRFVPNDTL
                     LDNHDNQLLIITGPNMAGKSTYIRQVALIVIMAQMGSFVPAAAAEIGVVDRVFTRVGA
                     GDDIARGRSTFMVEMQETANILNNATPRSLIVLDEIGRGTSTFDGISIAWSVAEHLHN
                     QASVKAKTLFATHYHELTDIALTLPGVKNYNVLVSEKDDRIVFLRKIVPGAADKSYGI
                     QVARLAGLPADVIARAKEILANLEEQELSEAGQPKLAQPRARKLRPFDGDQLSLFGN"
     gene            16913..17137
                     /gene="infA"
                     /locus_tag="PKI64_01310"
     CDS             16913..17137
                     /gene="infA"
                     /locus_tag="PKI64_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOD99676.1"
                     /translation="MAKDSNLIVVPGEVVKVLPATMYRIRLENGHEVLGHISGKMRRH
                     FIKITTGDKVTVEINPTDLTKGRITFREKT"
     gene            complement(17164..18024)
                     /locus_tag="PKI64_01315"
     CDS             complement(17164..18024)
                     /locus_tag="PKI64_01315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD99677.1"
                     /translation="MKRSAGPTPTLRRLPPVRRNGTPLLDHLAECLELSRRGAKNLLD
                     ERLVFVNGRRIWMARHLLQTRDVVELLPTATVRTAPAAPIRLLVDDSLFVVADKPPQL
                     LTTGERSLETRLREQLGVPALRAVHRLDKDTSGCVLLAKDDATRRALVAQFAAGTVRK
                     LYHALIAGNLPEPQMEIRAAVDNLTAVSRVRQVSFQSAPPRCAHVTVLIETGRTHQIR
                     IHLQHVGTPVLGDRQYFGSRSAAFSAVPRQMLHAHELRFSHPVGGRPVVAVSPLPPDF
                     RQWLRHLRLN"
     gene            complement(18021..18725)
                     /locus_tag="PKI64_01320"
     CDS             complement(18021..18725)
                     /locus_tag="PKI64_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOD99678.1"
                     /translation="MTPVQAPTAPRRWLAPIATVFCTVLVISNIAAIKPVALPGLPFV
                     LMDGGNVLFPISYIFGDILVEVYGYAQARRIIWLGFVLNLFAAATFVLVASLPAAPGW
                     ELQAAFTAILRQTPRVVLGSMAAFWCGSFLNAWIMAKMKVWMQGRHLWMRTIGSTIAG
                     EGVDSLLFTAIAFGGVWPLGLVARVSFWNFVLKTGYEVVATPLTYFLIGRLKRAEQSD
                     PYDVQTNFSPFRWRSQ"
     gene            complement(18722..19600)
                     /locus_tag="PKI64_01325"
     CDS             complement(18722..19600)
                     /locus_tag="PKI64_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99679.1"
                     /translation="MKKLWKILIGILVVLAIVAVVLELSLDKVVLKATNAAGPQLLGV
                     PVSLAKADISLFRGKAALGGLHVGNPEGFKTDGLLDLGSVAVRLDNKSLLSDTIVIKE
                     ITIDGLVVTYEQGLRGSNLGALIESLSDEEKEAEEKPEAEEKAGEEKPAKKVIIEKLS
                     ITGSRLNFSLTGAAALTGGGAVPIPLPPITLTDLGKDKDGATLLETIQSVLQAIAGAA
                     GSAIGGAGHLLGDGVQAIGGAALDTGKAVGGAAVDAGKAVGGVAADAGKAIGGAAVDA
                     GKAVGDTLKHLNPLKK"
     gene            19769..20710
                     /locus_tag="PKI64_01330"
     CDS             19769..20710
                     /locus_tag="PKI64_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOD99680.1"
                     /translation="MPYPIEKKFVVAVSSTVLFDLSYEHALYMEQGLEAFRRYQHEHR
                     DDLPKPGTGFPFIRRLLLLNQMYPEEHPVDVVILSRYHADDGMRVLAAVREYGLDITR
                     AFFMAGRKPYPFMESVNATLYLSTNAEQVREAVALGYPAGHVLPCEAVDDEQDDQLRI
                     AFDFDGVLVDDEAEKVYKQTQDLDKFRQYELENRDRPLKSGPLMPLFQQLAYFQKLDS
                     QRAQDDSGRKRLLRISIVTARGAPAHERLNNTLSSLGLEADEVFLMGGIEKKRVLDIL
                     KPHLFFDDQLAHLQPVARSTPSVHIPFGIANRGGEDS"
     gene            20753..21121
                     /locus_tag="PKI64_01335"
     CDS             20753..21121
                     /locus_tag="PKI64_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOD99681.1"
                     /translation="MSNAKLREAFHAAGMRLTRQRLLICRELANRYDHPDVEKIHQAV
                     RTRLKRISLFTVYRTVNVLEQHGLAWRVTTWKGHARYDGNTHAHAHFLCEECGRIDDV
                     DTQGACHEMAERAHECVPNR"
     gene            complement(21548..21865)
                     /gene="trxA"
                     /locus_tag="PKI64_01340"
     CDS             complement(21548..21865)
                     /gene="trxA"
                     /locus_tag="PKI64_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD99682.1"
                     /translation="MSAGILELTEQNFKSETQSGLILVDFWAPWCGPCRMQTPILEQV
                     VGKVAGAKIAKLNVDDAGSVAAQFGVQSIPTLIIFKDGQAVKQFVGVQQAPTLIAALE
                     AAK"
     gene            complement(21913..22104)
                     /locus_tag="PKI64_01345"
     CDS             complement(21913..22104)
                     /locus_tag="PKI64_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOD99683.1"
                     /translation="MTIENAVRVFAGIMILVSVALVHYHSPWWLLFTIFIGLNLIQSA
                     FTGFCLPSIVFRKLGLKDK"
     gene            complement(22169..22885)
                     /locus_tag="PKI64_01350"
     CDS             complement(22169..22885)
                     /locus_tag="PKI64_01350"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOD99684.1"
                     /translation="MEQQNTAPVSLPRIGDKAPAFKAKTTQGDIDFPKQYEGSWVILF
                     SHPADFTPVCTSEFITFASMEKQFAEANCKLVGLSVDGLYSHIAWLRSIQEKIEYRGH
                     KNVEVKFPLIEDITMNVAKAYGMIQPGEATTQAVRAVFFIDPKGIIRTIIYYPLSLGR
                     NFDELYRALIALQTADAFGIATPADWRPGDDVIISPAASCSSAKNRMDGKEDMVCQEW
                     YFCSRKLPKEEVLKKVLNKS"
     gene            complement(22931..23101)
                     /locus_tag="PKI64_01355"
     CDS             complement(22931..23101)
                     /locus_tag="PKI64_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOD99685.1"
                     /translation="MSPLLKTILGAAVGAAIGFAIYRFVGCKTGACPLNSNPWISMLV
                     WGIVGAITASGR"
     gene            complement(23136..23426)
                     /locus_tag="PKI64_01360"
     CDS             complement(23136..23426)
                     /locus_tag="PKI64_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99686.1"
                     /translation="MQMLMIICPENRRGEVRNLIADHGEGAFSEIANVFGSGKTGKHL
                     GSHTFPGQSVLIFVAIDEDKKAKLLAALKTFHDSLYSGEGLRVFALPVESIF"
     gene            complement(23429..26638)
                     /locus_tag="PKI64_01365"
     CDS             complement(23429..26638)
                     /locus_tag="PKI64_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD99687.1"
                     /translation="MEERKHSITSGILQAFLKGNLSILLILISLAAGAAALLITPREE
                     EPQIVVPLADVMVMMPGSSAEEVEQLVSSRLEKLLYQIDGVEYVYSMSRPGMAVVTVR
                     FFVGENREDSLIKVYNKIFQNIDQTTPGIAGWVVKPIEIDDVPIVNVTLHGAGADTHQ
                     LYRLAEEVAQKLQTIPDSARISIHGGQKRVVHVYLDTERLAAYGLSAMEVAGALKISN
                     AQMTSGTFEQADRAIVVEAGPFIENVDEVRNLMVGVHQNRPVYLRDVADIQDGSDELN
                     SYTRLGAGPAAAHVANGMAPGQSEPAVTVAVAKKKGSNAVNVAREVSRMISDLRGSIL
                     PDGVQATVTRDYGHTANEKVNDLVMNLAMGILTVVGLIALSMSWREGIIVGLAIPITY
                     SLTLLFNYLLGYTINRVTLFALILALGLLVDNPIVGVENISRFLSMKKYPRLRAVSLA
                     MEEVMPPILLATLSIIVSFIPMFFITGMMGPYMAPMALNVPLTMLSSLLVALAITPWI
                     SSKLLKEAPPEVAAYDVTATGTYRVYHRLMAPFVSSSGRSSLLLLIVAGLFGFSIFLA
                     ASGRVPLKMLPFDNKDEMQIVVDMPAGTTLEATDAAVREIERYLETVPEVVNFVSTVG
                     EGSPMDFNGLVRHYYLRQGPNMADVRINLLPRQQREMSSHAIALRIRNDIEAIGRQTG
                     ANLKIVEVPPGPPVLSTVVAEIYGRPDQPYSALIAAAEDLQTRMAAEQGVVDIDDSAE
                     AAQQKLFFRVDREKAGLNGVSTEDVVNTLRIALSGMPAGTVHAPREQNELPIVLRLPR
                     EKRSDPERLKTIMVKGRMGNSVQLGELGTFEENVVEPTIMHKNMERVVYVTAEMAGRG
                     PAYAVLNLQADLKKNPLPEGTHAVWTGEGEWKITVDVFRDLGIAFSAALLAIYVLLVY
                     ETGSYLLPIVIMLSIPLTLIGIMPGFWLLNAVGGATVGGYADSVFFTATAMIGMIALG
                     GIVQRNAIILIDFIRSEVARGKSLQDAIIEAGAVRFRPIFLTAGTTLLGAWPITFDPI
                     FSGLAWSIIFGLFVSTAFTLVVVPVAYGLIYRKPQPTEA"
     gene            complement(26642..27685)
                     /locus_tag="PKI64_01370"
     CDS             complement(26642..27685)
                     /locus_tag="PKI64_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD99688.1"
                     /translation="MKAAATLKKYLKPIVGIVGLAVVIAWSGGFLAKKVKPAKLEVLP
                     GLALPADAETIELKEENMPVRVEVVGTTASEEKINLSARIPAYVREVLASAGDRVKKG
                     QTLLTLDDRDIRQKLAAAEAQLAQAKTEFERAQQLFDKQATTDQALTAARSMFTAAQA
                     QVEEVKVMLTYAQVESPIDGVVTDRRIEAGDLANPGMLLLAVYDPARMRLEAPVPVRL
                     IDRLALGQEVEVALERPARVVKGTVAEIVSEVDSASRTQLVKVHLDGVNGDVLPGTFG
                     RLWVAAEPRAGLFVPASAVVQIGQLTYVQEARDGRALRRLVKTGPAHDGRVEILSGLR
                     AGNQIVVNPVREE"
     gene            complement(27717..>28034)
                     /locus_tag="PKI64_01375"
     CDS             complement(27717..>28034)
                     /locus_tag="PKI64_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD99689.1"
                     /translation="DEAKARLNLRLDLQQAFLGSQEAWERLEVTRKSLETAEEARRIV
                     QEQYQQGAADIAILLQTQVGVTAMQTRYAAAQYDYLTALSNLKRAKGMLGNEPAAETT
                     PNP"
     assembly_gap    28035..28134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28135..28730)
                     /locus_tag="PKI64_01380"
     CDS             complement(<28135..28730)
                     /locus_tag="PKI64_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD99690.1"
                     /translation="MKTMLFRGLMVAAALALAPVAQAVTLEQAVQAALDNSPTLQAAT
                     AKIDAAQAMLRQAKSYYYPSVGLAANYSVSDNPIQTFMMELNQRQMNMMDPGFNPNDP
                     EDTDNLRGSITAQWRLFDVQRAAGNEMARLGSQASAEAFAATRNQLVHDVTRGFYGVL
                     QAQAFAAVQEQAVQSIEESLRVARERFEAGGAVKTDVL"
     gene            complement(28730..29050)
                     /locus_tag="PKI64_01385"
     CDS             complement(28730..29050)
                     /locus_tag="PKI64_01385"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD99691.1"
                     /translation="MKTNSTPCPPLVSIADMARMAKVLKLLAHPYRLKIVDILDREKA
                     VPVHTLTQQLRQPQALVSNHLRKMLQAGLVARERRGKEVWYSVGDRRSLTILNCMRAK
                     KGVA"
     gene            complement(29151..29726)
                     /locus_tag="PKI64_01390"
     CDS             complement(29151..29726)
                     /locus_tag="PKI64_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99692.1"
                     /translation="MKTNCRLLALLTLCLLTASCMFPALAPWLTDETKVAAPDLAGTW
                     HDADIPCTVFFRPAETNGDYQVLMVNNLKDTSRFAASLHRVADHLLLAVGHENPEDLD
                     TFTLLPGYFLFQAEVADDVLRLYGLNLQNFRERALRAGLAVVPRGAQGNDEVLASPTE
                     AIAAFVQDQLAEPDFFDTTPLYTFHRLTAQP"
     gene            complement(29747..31267)
                     /gene="glpK"
                     /locus_tag="PKI64_01395"
     CDS             complement(29747..31267)
                     /gene="glpK"
                     /locus_tag="PKI64_01395"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOD99693.1"
                     /translation="MRTFILALDQGTTSSRALLFDRDLQPCATAQQEFPQLYPQPGWV
                     EHDPDVIWQSQYAVMEAALAQAGCSPADVAALAVANQRETVVVWDRRTGKPIHNAIVW
                     QCRRTAAACAALQEQGLAETIRAKTGLVLDAYFSATKIQWILDHVAGARAAAQRGELL
                     CGTIDTWLIWKLSGGHRHVTDFTNAARTLLFNIHTLQWDPELLELFDIPAAMLPEVLP
                     SSGIAATCALPGWAAHNIPIAGIAGDQQAALFGQGCFEPGQAKNTYGTGCFLLMNTGE
                     QAVESRHGLLTTLSAQTAPGAPAYALEGSVFIGGAVLQWLRDELGILANAAESAAIAA
                     ALPDTGGVYLVPAFAGLGAPYWDMYARGTLVGLTRGTGRPHIIRAALEAIAYQCADVV
                     TAMEKDAGRPLAVLRVDGGASANPFLMQFQADVLSCRIERPACMETTAQGAAALAAIA
                     LGWHTPADLARRPTARNFTPAMPAARREQLLAGWHQAVSRATGWATPIATSTPTPP"
     gene            complement(31365..32207)
                     /locus_tag="PKI64_01400"
     CDS             complement(31365..32207)
                     /locus_tag="PKI64_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOD99694.1"
                     /translation="MMQLVLVSLIWAFSFGLIGNTLAGLPSAWVGWVRLGLSALVFLP
                     FVRRVPARQAAFLVAIGAVQFGVMYWAYLSAFRFLQSHEVALFTVTTPILVALLADAG
                     SRRFRLFNWLAATLAVAGAAVVKWTELETAAPWRGFLLVQLSNLCFAAGQLAYRAVLR
                     RLPQPVVERKLFFWLHAGGMLALTPAALPWLRHTPTTSPAQWATLAYLGIVASGLCFF
                     WWNQGARRVSAGRLAVLNNLKIPLGVAVSLLVFRESAQLGVLLVGSGLLAVALVPVWR
                     QRSP"
     gene            complement(32636..33229)
                     /locus_tag="PKI64_01405"
     CDS             complement(32636..33229)
                     /locus_tag="PKI64_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99695.1"
                     /translation="MKKTLILTSLALLVASVAFAEKGSGRGEGKMRARAGDCPMAGTE
                     QPRWGQNDPAEAGRYAGGPLDAETMQKMRGLHKAMRSLGEAARLETDEAKKAELVAQL
                     RDKIGEAVDLAQASKEKRLAQAEERLAALKAQIESCKGDRDQLIEAQLQRILNNERPD
                     TREPFKKFPHAKGAGSESGHKHRGPKAAPEASPETTE"
     gene            complement(33259..33735)
                     /locus_tag="PKI64_01410"
     CDS             complement(33259..33735)
                     /locus_tag="PKI64_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99696.1"
                     /translation="MKPAQLEQLLLLEQSGELTPRQQAHLAAALADSAHARELRDQLR
                     HITRAVTAPEITPTMPASLADATARIDARLHPPRPLLAVAWKPLLAAAAALVLLVGAT
                     FFHIARHSPATAQRAALADDTELDTAWLDPMDAEFTELEDLLASIATDDFFLTMEM"
     gene            complement(33732..34343)
                     /locus_tag="PKI64_01415"
     CDS             complement(33732..34343)
                     /locus_tag="PKI64_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD99697.1"
                     /translation="MKTSGEHAINMVDDAKELAAYRRGDTAALGRLVAKYQRPLFSFL
                     SRFAANPDEANDWFQETWVRAIRNMNFFRQKNLPGWLFRIAHNLAVDAARRRRPEESL
                     DAAPPGSPAPLGELLPAAGLSPAAVVAGHDLGQQITAAVNRLPFEQREVFWLRMDAGL
                     PFKEIARLQRCSLNTALGRMQYALTRLRAALGPAYRELQDSAP"
     gene            complement(34515..35852)
                     /locus_tag="PKI64_01420"
     CDS             complement(34515..35852)
                     /locus_tag="PKI64_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOD99698.1"
                     /translation="MKFADAIPPAPRQILVTLRQHGHEAFLVGGCVRDLLLNRPPKDW
                     DIVTDALPDRIMALFPKTHAIGKAFGIITVVPEEGPPVEVATYRADAPYTDGRHPTAV
                     TFSDARTDAQRRDFTINALLLDPVSGELRDYVGGQADLAARLIRAIGDPTARFQEDHL
                     RLLRAVRFAATLDFALDPATRAAIPPLASQIHRISAERIRDELFRLLTESQQAGAALQ
                     LLHDTGLLREILPEVAAMIGVEQPPEFHPEGDVFTHTRLMLDALPPRPSLRLALAVLL
                     HDVGKPPTAHYATLRDGTQRWRFESHASVGADMARAILTRLRAPNALTEEVATIIAGH
                     MRLADAPNMRPAKLRRLLGSPLFADELELHRLDCISSHAQLDIYHFLQNAHAQFAAEP
                     VLPPPLVTGRDLIALGHTPGPHFSEILQAAYDQQLEGQTDKAALLAAIPVPPP"
     gene            complement(35849..36631)
                     /gene="xth"
                     /locus_tag="PKI64_01425"
     CDS             complement(35849..36631)
                     /gene="xth"
                     /locus_tag="PKI64_01425"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOD99699.1"
                     /translation="MTRIMRIATFNCNSVRSRLDAVLRWLSDYTPDVLCLQETKVTDD
                     LFPTDAFTAAGWHVAFKGEKSYNGVAIVSKQPADEVSFGLDSEPADPTRLIHARFGEV
                     HVVNTYVPQGRDIEHEMYAYKLEWLARLKQYFQRRQAPTRPLLWCGDLNVARHPIDVS
                     NPETKKDHVCYHHAIREAFEDVLALGFTDLFRRHNPDLVDYSFYDYRVPFNPEQKRGW
                     RIDYVLATPPLAAKSHAAAIDLTPRTRPKPSDHCPLYADLRA"
     gene            complement(36678..36941)
                     /locus_tag="PKI64_01430"
     CDS             complement(36678..36941)
                     /locus_tag="PKI64_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99700.1"
                     /translation="MSIFEAGMLICFGISWPISIAKSIRTKVVVGKSPLFMAILCLGY
                     ASGIAHKWLYSRDPVIALYTLNLLLVATDLALYFKYLPRAARA"
     gene            complement(36963..37610)
                     /locus_tag="PKI64_01435"
     CDS             complement(36963..37610)
                     /locus_tag="PKI64_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99701.1"
                     /translation="MRCYWLLLLVLFLTLPPARAECLPLDPVTLGGAEIFAAVEQDIA
                     AAATTAGVVFTPDSRPLPPQVILTFDTPDGRIQQKHGVLFWRGEMLPDQLAPGDIGTW
                     IRRCRTTASAWKTVHTEPHAAPDAHTNVVPTACAVAPAPLPTMVLETPYQIGTIAGQP
                     VRLVLWRDTTEGSAPLGGFLDLPAAAPAIAAALQTRYAPFPERDAWASLFNALRP"
     gene            complement(37613..38167)
                     /gene="folE"
                     /locus_tag="PKI64_01440"
     CDS             complement(37613..38167)
                     /gene="folE"
                     /locus_tag="PKI64_01440"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOD99702.1"
                     /translation="MQDQFRQILAAMGEDPTREGLLATPQRADEAWGYLTRGYRQDPA
                     ALLQSAIFEVEANHMVIVRDIEIYSLCEHHLLPFFGVCHIGYIPRGKVLGVSKLARLA
                     DLYARRLQVQERLTNQIARTIMDTLRPEGVGVIIEAQHLCMMMRGVEKQGSRMVTSAM
                     LGSFHDSVATRNEFLHLSRHRSSE"
     gene            complement(38226..38729)
                     /locus_tag="PKI64_01445"
     CDS             complement(38226..38729)
                     /locus_tag="PKI64_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOD99703.1"
                     /translation="MNIKKSIPWLLLAALIFVMPLSSCNAFEWRTDLAAAASDAQTGQ
                     KLILLNFSGSDWCGWCKRLDAEVFSTSEFKDYAAANLVCVIADFPRGTKLPPALQAQN
                     ERLMRAFRVEGFPTILVFNSAAELIGQLGYQPGGPAAFINSLKQVQARAAMRPAGSPA
                     GPKLPLE"
     gene            38829..39719
                     /locus_tag="PKI64_01450"
     CDS             38829..39719
                     /locus_tag="PKI64_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD99704.1"
                     /translation="MFLGLLQMLSKVVAALPKRGAEAIARHWGWLLAHVIRLRRRYVL
                     ATLARSFPEKSAAECRAIYNEVCRQQALNAMELLRYAGGRQAELAAQFEAVGLENLAR
                     AQTAELGALVLMGHFGNYALLALQVPRLFNLPLAIVAKPLRNATLNDQWWELMRKAGI
                     TGIPARNAYRASVRALKEKKLVGFMLDQNRPATQGVFVDFFGKPAATTPGLALMSAQT
                     GAAVLPAFVHRLPDGRHRVVVHEPIAPPPDRRPETIREYTARYTRILEDEIRREPAQW
                     LWWHKRWKSRPPAEQPPVAP"
     gene            39716..40684
                     /locus_tag="PKI64_01455"
     CDS             39716..40684
                     /locus_tag="PKI64_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99705.1"
                     /translation="MSNARTKNAEPLWRRLRMTLLEVPATRLALAIVPRLPRGAVLGL
                     ARFAANAGYACSPAMRRLGLTNLDLAFGDTKTPAEKRRILRESMRNFMLVLLDTIWFS
                     RDSVARLQRWFRAAPSLQAVMAQREATVGITGHFGNWELTGRYWAEQTGGLMSVAMPL
                     KNPAVDKLVTRVRQLNNQIVVPRDGALRKLVGFLRAGGTIGLLLDQNTPPREGGIFAN
                     FFGKPVAVSPAAGVLATMTRARILFGYTLPQPDGTYLAEAPRAIAAAEISAMDRKTAT
                     ADLTQRINDFYEEAIRAHPECWLWTYRRWRYMPPGAVREDFPYYAK"
     gene            40697..41032
                     /locus_tag="PKI64_01460"
     CDS             40697..41032
                     /locus_tag="PKI64_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012007685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HOD99706.1"
                     /translation="MLNFATYQQQAMTTAVYPGRGEGNFTYPALGLAGETGEVCEKIK
                     KAIRDDGGVISAERRALLAKEIGDVLWYLATLSTELGLELQEIAEQNLAKLAARKANG
                     HIHGSGDLR"
     gene            41034..41723
                     /locus_tag="PKI64_01465"
     CDS             41034..41723
                     /locus_tag="PKI64_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOD99707.1"
                     /translation="MARPAAVIFDFDGVIVDSERLHHETMVAAMAGQGPATPWEFYHE
                     HLMGFDDRGAFAVLLERAGIAPTPEEIQRRIAVKARLFAEAAAAGRVPALPGAVELVR
                     ACAVQGPVGLCSGALRSDILPVLDALGIADCFAEMVTADDVPLSKPDPACYRLCVARL
                     AARFPEEGITPATCVAIEDTTDGISAARGAGIPVVGVATNMEPALLLAAGARRVFVAL
                     TAITPEQLAGG"
     gene            complement(42165..42683)
                     /locus_tag="PKI64_01470"
     CDS             complement(42165..42683)
                     /locus_tag="PKI64_01470"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOD99708.1"
                     /translation="MHLRYVNKLGIPKDIKLTGEPLAIGRAREADIPLLDDKVSRIHA
                     GIRLSDGEFYLKDLKSRNGTYVNGQRVEDTVKIKAGDRIQIGATIFVLEAASTAEDAQ
                     KGLEAVKDAMADGKGYTTILKEIVEDIVPTEGAAQTKPPAPAESKPAAAPPAASKPSG
                     RPAIKITIKKPT"
     gene            complement(42698..43657)
                     /locus_tag="PKI64_01475"
     CDS             complement(42698..43657)
                     /locus_tag="PKI64_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD99709.1"
                     /translation="MKSERPITLVTGGAGFLGSHLCDRLLAEGHRVVAMDNLITGNLD
                     NIAHLRSRPDFEFIHHNVTTHINWSGPLDYIFHFASPASPIDYLELPIQTLKVGALGT
                     HNALGLAKAKGARLLLASTSEVYGDPLVHPQVESYWGNVNPIGPRGVYDEAKRFAEAI
                     VMAYHRTHGVATRIVRIFNTYGPRMRPKDGRIVPAFCCQALKGEPITVFGDGSQTRSF
                     CYVSDLIDGIFRLMMSDYAEPVNIGNPRELSVMDFAVKIKELTGSAAPIVHKPLPVDD
                     PKVRQPNISKARALLGWEPKMPLEEGLRLTIAYFAELIQKGIV"
     gene            complement(43677..44447)
                     /locus_tag="PKI64_01480"
     CDS             complement(43677..44447)
                     /locus_tag="PKI64_01480"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOD99710.1"
                     /translation="MDNLQKSFFGPRLFVIAALAVAAWALVYKTGVASTDEAGIVLAL
                     PARVGPWEGVELLFCTDRNCNRQYAANAVPDNAQCPNCGAPLSNMNWAERSMLPADTG
                     LVRKYYSLPNGTSGVHATIVLSGDDRSSIHRPQVCMTAAGHEIVSSRLIHVPLPNRAE
                     PLEIMVLEMTRSYRDENGNPADLNTYYAYWFVGKGRETASHLKRMFWMGYDRVVHGVS
                     HRWAYIALSGPRNPANENHLQAIATFASQLHPALLKPE"
     gene            complement(44402..45373)
                     /locus_tag="PKI64_01485"
     CDS             complement(44402..45373)
                     /locus_tag="PKI64_01485"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOD99711.1"
                     /translation="MQNQGQQLKAWWAGFRWTRLTRAAQLEIGFWTVVVAGLFFLFHL
                     HGNSQEVATCRRSLFLWLNSRWVGDYAYCMVLPLASLLALWIRRREIAAAPRRANWWG
                     ALLIVGALAIHWVGVLAQQPRLSAGGLIILLWSLPFLIYGWELARWLLFPCAYLFLAI
                     PLNFIDSMTAPLRLFATMVAGGILNGFGLEVQRVGAGLFSGAGNSFAFNVAPECSGLR
                     SLLAMTALMGFYAWYSQKTLAKKWLLFFLSIPVAIVANICRVILVVVVAAFWGQETAM
                     GLWHDYSGYPIFIISIILMLTLDRLLNMDYRRAWTICKNRFLDRASS"
     gene            45482..>46176
                     /locus_tag="PKI64_01490"
     CDS             45482..>46176
                     /locus_tag="PKI64_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOD99712.1"
                     /translation="MTKPTGKIWLLLTLLALAAPLGRAELVREELPDASDWVHDIVFE
                     HEAGADDGTLSVRLGTDPVTVRYFEVPLSVWAEFKAAESKGSFYGLNIRQRYERQYGK
                     SRQEVFDSPLPIQTQINALCAFNEECEDVVLEGIEKSRESIWVAAYAFTRTRIAAALI
                     RAHQRGVRVAVKMDVNQAEFAGAQKLIEWLRREGIPVTLIRTAGDYSAMHNKFMIFDM
                     RWVVTGSYNFTTTA"
BASE COUNT         8456 a        13660 c        15261 g         8699 t
ORIGIN      
        1 gacctaaatc ttagaaaatt cgatgggttt aacctcgaat agcgaaagac gggttgggtc
       61 ggcgtgttat tcggctgttg gctgttcctg tagccggcct cggtgaggcc ggggctgctg
      121 gttcggaggt ttttacctga catctgaacc ctgaaccctg aacccttttt tgcattccgc
      181 tttacaaaat cgcgatgggg gagtagaagt tggactccca aacataaccg agcccatgcc
      241 atgtcaggaa acgtaatgca accagtcatc aaccttctcg tccaacttca agagctgatt
      301 atcgctcgcg cgcagcatga agccggacaa ccgggcgccc aacttgacca gttggatgcc
      361 tccatcgctt cgctgaaaaa agaaatccct gcggatgtcc gccagcgttt tgagcgtcaa
      421 ctgcaaaaaa attcgctggc gattgtgccg acggaaaacg gtgtttgcac cgcttgcggc
      481 atgacgctgc cggtggcgct ggtgcaccaa gtgcacgccg cggagcaaat ctatggctgc
      541 ccgtcgtgcg cccgcctgct gttctaccgc agcggcagcg cgcgcaacac ccgcaagccg
      601 ccgcgtcgca gcgatccgcc caaggtcggc atcgaacgct tttcctccga gaccttgatg
      661 attcccgcac tcaagggcac cgaccgcgaa agcgtgctgg cggaactctg ccagaagctg
      721 caagacgaag gttttgtgga caatgcccag cagttgcagg aagctgcgct ccagcgcgaa
      781 gccatcgttt ccaccgccat cgaaggtggt gtggcgttcc cccacgtgcg ctgcgtggaa
      841 ggcggcgcgt tgacgctggc gttgggcatc gcccccaaag gcatcaagtt tgaccccaat
      901 ggcaaagcca acacccgcgt ggtgtttttc atggtgattc caacggccgc aagcgccttc
      961 tacctgaagt tgctttccgg gttggcgcag gttttccaga agccggaaaa ccgcgacaag
     1021 ctgctcgaag cggataccgc tgagaagctg tggaaggcct tggtcaaggc cacccgcacg
     1081 gtcattcaat agcggcctcc gccgcgcgcc cggtgattcc gcgccgcaat ctggtgttgc
     1141 tgggcttcat gggcaccggc aaaagcacgc tgggccggcg cgtcgccgcg ctgaccggcg
     1201 cgccttttct cgaaatggat gccgaactgg agcggcgcgc cggcaaaccc atcccccgga
     1261 tttttgcgga agacggcgag gccgcgttcc gcgacatgga aaccgctttg gcccgcgaat
     1321 ggggccaaca ggaaagcgcg attatttcct gcggcggcgg cgtggtgctg cgcgcggaaa
     1381 atctgacggc gctgcgcgcc aacggtgttt tggtttgttt gacggcgcgg ccggaagtga
     1441 ttgtggcgcg cacggcgcgc gcgggcaacc gcccgctgct ggccggcgac gatcctgccg
     1501 ccaaagtgcg cgagttgctc gccgcccgcg ccgcgcacta cgcgcagatt ccgctgcaac
     1561 tggagacgtc ggatgccgac ctgtccgacc tcgcgcaaac cctgttggat ttgtggttac
     1621 gggcctgatg catgtggcgc tatctcgcca ggcgactcgc gcaggccgtg cccacggcgc
     1681 tgggcgtggt gctggtgacg tttgtgctgt tcaacgtggt agggggcagt cccgccgcgc
     1741 aggtgctggg ccagaacgcc gggccgctcg cgctggaaga atttgatctc gcccgcggct
     1801 acgccaagcc gcttttctgc ggccggtgga ccgccacccg cgcgctggcc gccgtggatt
     1861 tcgccaccgc cgcgcccagc gtgcgcgcgc aatggcatgc cggcgattcc ggctggctga
     1921 gcttggctga tggcgaagaa ttggtcctgc cgttggcgtt tccgctgccg gcggaaggta
     1981 catggcggct gacggtggag gggcgtggcc ccggttttcc acagtgtgga aaaaagtttt
     2041 ccacagtgtg gaaaaaaagc gccaaaagtt ccacggtgtg gaaaaaagtt ttccacggtg
     2101 tggaaaaacc caccttccgc gcggcgggcg gcgcggtgga aatccggcgc gtgcggctgg
     2161 aacgcggcac gcggcatttt ttcgacagcc aactgacgga ctacatcggc cgcgtggccc
     2221 gcctggacct tggcaccagc gccagcttga accagccggt gacgcggctg ctgcgcgcgg
     2281 gaatcgggcc gagtcttgcg ctgactgtgc cgattttgct ggtggaattg gtggtgagcc
     2341 tgacgctggc gctgctgtgc gcttatcgtc gcggggcgtg gctggaccgg ctgctggtgg
     2401 cggtgtgcgt ggcgctgatg agcgtgaatt atctggtgtg gattgtcgcc gggcagtttt
     2461 tgctggctta ccgctggggc tggttcccgg tgtgggggtt tgaatcgtgg cgctatttgc
     2521 tgctgccggt gttgattggc gtggccacgg ggctgggcgt gaatgtgcgg ttttaccgca
     2581 cgatcctgct ggaagaaatg cacaaggatt acgtgcgcac tgcgtttgcc aaggggctgc
     2641 gcacggagcg ggtgctgttc aagcacgtgc tgaaaaatgc gttggggccg gtcatcacca
     2701 acgtggcggt ggcgctgccg ttcctctata cgggcagcgt gctgctggaa aacttttttg
     2761 ggattccggg actgggctat ttgagtttga acgccatcca ttcggcggat gtggacgtgg
     2821 tgcgcgcggt ggtgctggtg ggggcactgc tgttcacggc ggccaacatt ttggcggatg
     2881 tggcgctggc gtgggtggac ccgcgggtga agctggcatg aacaacgcgg cggaacaaat
     2941 gggtgcgggc gcgtcgttcg ggcgcgaggc atggcggcgg gtgtgggcca gcggcattgc
     3001 ccgcgcgtgc ctcgcggtgc tggcgttgtt ttttgtggtg gcgctggcgg gcgaggccgc
     3061 caatgcgtgg tacgagtggc gcgatgtcac gccgccatgg caggcgcagg agctggccgc
     3121 gcgctacctg ccgccgggcg cgggccactg gctgggcacc gatggccttg ggcgcgatgt
     3181 ggccctgcgg ctggcgcagg gcgcgcggat cgcgttcgag gtcggcgtgg tgtcgtcgct
     3241 actggcggtg atgatcggcg tgctgctggg ctgccttggc ggctacttcg gcgggtgggt
     3301 ggatgatctg gtggtgtggc tggccaccac gttcgcagcc attccggggc tgctgtttgt
     3361 gctcgccatt gcgatggtgg tgggcaaggg gctgctgggc gtttttctgg ccatcggttt
     3421 gaccacgtgg gtgggggtgt gccggctgat ccgcgccgaa gtgctgcgcc agaaagaacg
     3481 cggctatgtg cgcgcggcgc aggcgctggg attttcgtcg tggcgcattt tgttccggca
     3541 cattttgccc aacgtgctgc acgtggcgct ggtgtcgttc acactgcgct ttccggcggc
     3601 catcggcacg gaggttttcc tgagttttct ggggatcggc gcgcaggggc agcccagttg
     3661 gggaaccatg atcgcgtccg cccggctgcg cctgtggcag gggatgtggt gggagctggc
     3721 cgcggtcacg gtggccattt ttgcggtggt actggcgttc aatctgctgg gcgatatttt
     3781 gcgcgatgcg cttgatcccc ggctgcgggt cgcgaccgat tgaccgcccc cgccggcccg
     3841 cgcccgcgca gccatttttc gggccggcgc ccttgacacg cccccgccgg cgtggtttat
     3901 tccccgcacg atgacaacga aatccagttg gaacctgctt ctcgcgttgc gcggcgacac
     3961 ccgtgcggcg gcgggcgacg ggtgttgcat ggtttgattg tcccgaagcg cgttgatttc
     4021 tggaaagccc gccaactttg aacttggcgg gaattttttt gcccgcccga ctggaacccc
     4081 aagaggaaaa taaaaatgaa aaagtccgac catgtgattg tttttgacac cacgctgcgc
     4141 gatggcgagc aatgccccgg cgcgagcatg ggcctgcacg aaaaattgca ggtggcccgc
     4201 caactggaaa agctgaatgt ggatattatc gaagccggtt tcccggcggc gtcacccggc
     4261 gattttgagg cggtgcgcca agtggccgcc accatcaaaa agtgccggat tgcgggactg
     4321 gcgcgctgcg tcaagagcga catcgaaaaa tgcgccgagg cgctcaagcc cgccggcgac
     4381 cgcgcccgca tccacgtgtt tctggccacc agcgccatcc accgcgagtt caagctcaag
     4441 aaggccaagg atgaaatcat ccggcaggcc gtcgagggtg tgacctatgc gcggcagttt
     4501 tgcaaggatg tgcagttcag ccctgaggat gcctcgcgca cggagccgga atttctggcg
     4561 gaagtggtgc gcgcggtgat tgccgccggc gccagcacga tcaacattcc cgatacggtc
     4621 ggcttcaccg tccccaacga gttccgcaag ctgctgactt acctgaacga gcatgtgccg
     4681 gagctgcaga acgtggtggt gcacgtgcat tgccacaacg atctgggcct cgcggtggcg
     4741 aactcgctgg cggctatcga gtgtggcgcg cgcggcgtgg aatgcaccat caacggcatc
     4801 ggcgaacgcg cgggcaactg ctcgctggag gagctggtga tggcgctgaa ggtgcgcaac
     4861 gaccactacg gcatccaaac ccgcgtgcgc acgcaggaaa tttttgccac cagccgcctg
     4921 gtcagccaac tgaccggcct gatggtgcag cgcaacaagg ccatcgtggg cggcaacgcg
     4981 tttgcccatg aatcgggcat ccatcaggac ggcatgctta agcatcgtga gacgtatgaa
     5041 atcatgcggc ccgaggatgt cggcgtggtg accggcaccg acctcgtgct gggcaagcac
     5101 agcgggcggg cgggcttctc ggcgcatctg aagcgcatgg gcatccaact caaggccgag
     5161 cagttgcagg gcgtgtatga tgcgttcatc gcgctggccg acaagaaaaa ggtggtgtac
     5221 gacgacgaca tcatcgagct gctgcgcgaa tacgtgtcgg agattccgcg ggtgtacgcc
     5281 atcaagtgcc tgcaagtcac caccggcaac cgcgcgctgc cgacggccac cgtgcagatc
     5341 gaaaaagacg gcaagatcat cgaggattcg gccacgggcg caggcccggt ggaagcggcg
     5401 ctgaaggtca tcgagcgcgt cacgggcatc aagggcgagc tgaagtcgtt tgccatgcag
     5461 gcagtgacgc acggcgagga tgcgctgggt gaagctgcgg tgcaggtgaa gtttggcgat
     5521 gatctcgtgg cggccaaggg gtcggacatt gatatcatcg tggcctcggc caaggcgtac
     5581 atcaacgccg tgaaccggca tctgtttttg acgcaaacca gcgccaagct gtaagcccgc
     5641 gtgcggccat agctgaggga cgtgaccttc ctgccgggaa cggctggaag gtctgcgacc
     5701 attccagccc aacaaatcaa cccggatgtt tttgtagggc gggaatggtt gaaggatgaa
     5761 accttcccgc cgagaacggc tggaaggtct gcgaccgttc cagccctaca atttcaaccg
     5821 ggagtttttg tcgggcggga atggttgtgg cacgatactt tcccgccggg aacggctgga
     5881 aggtcggcta ccgttccagt cttacaatcc aaccgtcagc agtttttttg tgcgggacgg
     5941 aatctcccgc tgggggaatt gttaggtttt gatttagatg ttgtgcgggg tccgcataaa
     6001 cggcatatac gaacaatgcc gtcaaccccc ggacatttga ttcgcctgct ggtgtggctg
     6061 cttctcgcgt tggtgggcgc ggggctggcg gtctggtcgt ggccgggcga gggcgaccag
     6121 ccggtgtatc ccggccggca ttggtcgtgg atggcgccgg agcgtgccgg gtattccgcc
     6181 gcgaagttga acgccttttc ccggcacaca ggcggctcgg ggtgcatcgc gtatcaaggg
     6241 cggatgatct acgcgtgggg ggattgggcg gcgcggggcg acgtggcctc cgtcgcgaaa
     6301 ccgctctatg ccttcctggc ctacaaggcg gtggagcagg ggcggatcgg ctcattggat
     6361 gatttggtgg ccgactgggt gccggacaac acggcgctgg atgagtacct gaaaaaaatc
     6421 gcggggccga ttacgttccg gcaattgctg gatcagacgt ccggctacgg tttggaagaa
     6481 agccccggcg aggcgtttgc ctacaacgac tacgcgacgt atctctgcgc gtggctgctg
     6541 ttcgaccgcg tgtgggagta tcccgaagcg gacaatgatg ccgtgttgaa cggcgaatta
     6601 ctgggcggtt caatcggctt tgaggaccag cccaccatac agcaccggca gtcgcgcccg
     6661 ttccggctgc ggatttcgtc gcgggatttg gcgcgctttg ggctactcta tctgcgcggc
     6721 gggcgctggc ggaaagcaac gctgctgcgg gcggatttat ttgccagcca catgcactcg
     6781 gcgttgccgg cggattttcc ccgcaccagt ggcgaggagg atgagctgct gccggaggcc
     6841 cggaccgttg gcggcgacaa agatgaaaag catcacttgg ggagcctcgg ccaattctgg
     6901 tggttcaacc gcgtcacgcc cgatggccac cgtctattgc ccgatgcgcc ggaggatacg
     6961 tttttcggtt ccggctacgg cgggcggttt gccttgctgg tgattccgtc gcaggagctg
     7021 gtggtggcgt ggcaggatat ttatccggac gaggactgga ttccgctgga cgaaaccggg
     7081 cggcaccgcc tgaaccaaac catccgcgcg ctacttgccg cccgcgtgcc ggcggagtag
     7141 gccgcaacat cagccgccgg aaaacggcgg caggaaggtc acttggtcgc catcgtggat
     7201 gggggcgtcc caatccacga attcatcatt gatggccgct ttgacgatgt tggtcgtcag
     7261 cgggaaccgc ccggtctgct ggatttcgtg cagcagttgg cgggtggttt ccgcggtggt
     7321 ctcatagggc tcggtctggg tgccgcgccg cgcgccgacc aaggcaaagt agcgaaccgt
     7381 gacgtgtttc aagctcatgc tgtttgcgca gccttcaggc gggcttcgag atcgttgagt
     7441 tcctgctgca gggcggcggg aatggcatcg ccgaacttgg cgtagtgcgc gcggatggcg
     7501 gggatgtcct tgagccagcc ggccacgtcc acttccagca actcggccat gtcgtccgcg
     7561 gcgatgtcca agccggtggt atcaatggcg ccgggggcgg gtaggagacc cagcggcgat
     7621 tccatcgcct gaccgcggcc ttccacgcgc tcgaaaatcc atttgagcac gcgtgagttt
     7681 tcaccgaagc cgggccacag ccagcggcca tcgtcggact tgcggaacca gttgacgtag
     7741 aacacgcgcg gcattttggc gttgggcaca cggccgggca tggagagcca atgggcgaag
     7801 taatcgccca tgttgtagcc gcagaagggg agcatggcca tggggtcata gcgcaacaca
     7861 cccacgctgc ccgcctgcgc ggcggtggtt tcgctggctt gaatggagcc gaggaatgtg
     7921 ccgtattgcc aggtgggcgc ctcaaagcag agcggcaccg tgctggggcg ccggccgccg
     7981 aacaggatgg cagaaatcgg cacgccggcg gggtcttccc aggcggggtc aatcaccggg
     8041 cattggcggg cgggggtggt gtagcgcgcg ttggggttgg ccgccttgct gccgctttgc
     8101 ggggtccatt cttcgcgctt ccacgaggtc agtttgggcg gcggggtgtc ggtcatgtcc
     8161 tcccaccaca cgtcaccatc gggagtcaac gcgcagttgg tgaagatgga gttggcatag
     8221 agcgtgcgca tggcgttggg gttggacttc atggaagtgc ccggcgccac gccgaagaag
     8281 ccggcttccg ggttgatggc gcgaagctgg ccgttggcgt cgaatttcat ccagcagatg
     8341 tcgtcgccca cgcattccgc cttccagccg gggagtgtgg gttggagcat ggccagattg
     8401 gttttgccac aggcgctggg gaatgcggcg gccacataga tttttttgcc ggcgggattc
     8461 gtcaggccca ggatcagcat gtgctcggcc atccagccct gccggcgggc catcgtggag
     8521 gcgatgcgca gcgcaaagca cttcttgccc agcagcgcgt tgccgccata gccggagcca
     8581 aacgaccaga tggaggggtc ttccggaaag tgggagatgt atttgttgtt tgcgttgcag
     8641 ggccacggca catccttctg gcccggcgcc agcggcgcgc ccacggagtg caggcattcc
     8701 acgaagtcgc catcatcgcc cagcgcggcc agcacctccg cgccgatgcg cgtcatgatg
     8761 cgcatgttga ccacgacata gatcgagtcg gtcagctcca cgccgatatg cgcgatgggc
     8821 gagccgatgg gccccatgct gaacggaatg acgtagagcg tgcgcccgcg catgcagccg
     8881 gcatagagct tgcgcagcgt gtccttcatt tccaccggat cgcgccaatt attgttggga
     8941 ccggcctcgc gcggatcggt ggtacaaaca aacgtgcggt cttccacgcg ggcgacatcc
     9001 gccggatccg agaacaccgc gaacgaattg ggccgcttgg ccgggtccag ccggatgaac
     9061 gtgccggcgg cttccgcctg cgcgcacaag cgctcgtttt cttccgcgga gccatcgcac
     9121 cagtaaatct gatcgggctg gcacagttcg gcgacttcgg ccacccactt cagcagtcgt
     9181 tggttgttcg tcttcatctc ggtatccttc cgttacggaa cgggttcctc gcggccagcc
     9241 ggtccatcgg caggcttcag gcacgtcatc ccggagccgt cgcacgatac gggctaggcc
     9301 ggcgggccgc aagtccaaaa acatgaaaaa agcgcgcggc ttgcatttcg gggctcgatt
     9361 tgtttttctg cgtcattccg tgagaaagag cgcgcatgag tgagatgaac aacgcggggg
     9421 acttggaaaa ccagttattg gaactattga cgcaggactc agcgcccgcg gccgcttggc
     9481 tggccgtgtt gacggccgga gtcacggcgg ggattccggc cgcgacgcgc gagtgggcgg
     9541 ccatggcgca ggaggcgctg gccaaggcgg gggatgtggc cggcggcagc gatttgttga
     9601 agtggcgcgc ggacaatacg cccccggatc agatgaccgc caaggattgg ctgaaggtag
     9661 ccgacgtgct ggccgggtcc gatccctatt tgctggcgtt gattcaggag gcggggttcg
     9721 gccagaagtt ggcggcccgc gaatgcgtgc gccggctccg cctgctgttg agtttgcagc
     9781 ctggtgcgct gtgcctgcat cgcacgtggg ggtttggcgt ggtgcagaaa gccgacgcgc
     9841 tttaccgcaa gattgaaatc aatttccggg gacgccccgg ccacgggctg gccatgaaag
     9901 tggctgccga gagtctcgaa gtactggccg aaacccacct gttggcccag tggcatcgcg
     9961 accgcgaacg catccgcgcc ctggtgcgcg acaatccggc ggaagtggtg cgcttggcct
    10021 tggccagctt tggacccatg ccggtggcca cgctgcaaaa aacgctggtc agcgccgagc
    10081 tgttgccgga tagcgactgg aaacgtttct gggatgccgc ccgcaaagtg ctcaaggccg
    10141 atccgctggt catggtgccc gccaagcgta cggagccgct gtgcctgctg aacagcgcgg
    10201 ccggatttga cgacacctgg tttgaccggc tggccaacga gcgcgagctg aagaccatcg
    10261 tggagcggct gggcgaactg gccgaacgcg cgcccgccca cgacaccatt cccgctgcgc
    10321 aacggcaggt gatgaccaac cgcttggcgt ttgtgctgca tagcgccacc agccgccagc
    10381 cgggcatcaa gctgcaagcg gccctgctgg ccgcgcgcct cgccgtgccc cctgcggagt
    10441 gcgactggcc cgccgccgcc cgcgagttcc tgcgcggccc cgcccttgtg gaagtgctgc
    10501 acgatctgcc cgcgcgcaaa gtcgctcccg cgctggagtt tttgtgggcg caggatccgg
    10561 aggcgttgcg cgctgcgctg ctcgaacaac tgggcaattt atacttcggc ccattgcagg
    10621 aagtcatggt cctactgcgc aaacagggga gcgacgaagc ctgccgtgat atttttgccg
    10681 caacctgcgc cgcacacacc atccggcagg aaatgctgtt atggctgctg cgcaacgtct
    10741 ccctgattgc cgagtgggat ttgcccttgc cggccgcgct ggcgccaatc gtgctggccg
    10801 agttggagca ggactacaaa ggcgaacggc tgaaaacccg caagcaactc cgcgaagcat
    10861 tcgaagatgc ggcatggctc aaggcggtgt ttgacggcat gacgccctcc cgccagcagg
    10921 atttcttccg gcgcttgaat cagtcgccgg cgtggccggg cctggaccgg caagtcttgc
    10981 aggccaagat actcaagcaa tttccgcagc tccatggcgt catcatgggc gcggcggcgg
    11041 agacaccgac ggccagctcc gggcaggtga cctcgcaccg ctcctaccgc gagcggcagg
    11101 aaaaactgga acgactgctc aacgtggaaa tccccgccaa ttccaaggaa atcgccatcg
    11161 cgcgcagcta tggcgatttg agcgaaaact tcgaatacaa ggcggccaag gatatgcagc
    11221 gcgtgctgat ggcgcggcag gccgatttgg aaggcatgct ggccaaggtg cgtcccaccg
    11281 attttgccga agcgccgacc gaccgcgctg gcgtcggcac gaccgtcacg ctggcctatc
    11341 cagacggtca tcgcgagttt ttccacatct tgggcgagtg ggatcagttc cccgaaaagc
    11401 atattatttc ttccaacacc cggctggctc aggcgctggc cggcaaacag gcgggagagg
    11461 atgttgaagt tccggcggaa gatggcccgc cggtcacttg ccgcgtggaa accgtcacgc
    11521 cgttaccgga cgacatcaag gaatgggtga agtgacatga ccttgcgcga acgctggcag
    11581 cattatcttt ctcacgacgc gcccccgctg gtgcagtttg tgaagtacgg cctcgcgggc
    11641 ggcatcgcca ccgtcactca cgtgggaagt tttttcctcg caggtttctt gttgttccct
    11701 tgcgtggggg ccgatgaccc gctggtgagg ctgttcaacc tggccgcgcc ggtggtgaac
    11761 gaagccctgc gcgcgcgcta cgcggtatat tccaacctca ttgcgttcgg cttttccaac
    11821 accgtttgct acctcatcaa ccggtggttc gtgttccgcc ccggtcggca ccatgtggtg
    11881 ttggagtttc tgctattcct gggcgtgtcg gccatcagca tgactgtggg caccacgttg
    11941 atggggctgc tgatcaaata ccttgggatg ccaaccacct acgcgtttgg cgccaacatc
    12001 ctgagctcgt tggctattaa ttacgtgctg cgcaaatatt tcgttttcaa aggataatcc
    12061 gcgcaaacgc gcgggttggc gcctggcccc aaaacctgat tccagttgga ctgtcgggct
    12121 ggaatgattg cagaccttcc agccttttcc ggcgggaagg tttcgttcct caaccattcc
    12181 cgccctacaa aagcactgct agaacagcca tgccgcatcc gggttgaatt gttgggccag
    12241 aactgtccgc gccttgtctc gatcacaaaa aaacgcccgc tctttttggg agcgggcgcg
    12301 tcgttgcggc gtgagccgcc gcgtttacat ttgcgtggaa aggaattccg cgcggcggtt
    12361 caagcgccac gaggcttcat catggccgaa cgccaccggg cgttcttcgc cgtagctacg
    12421 ggtttggatg cgggcgccat ccacgccggc ggcaatcatc gcggcgcgga ccgcgagggc
    12481 ccggcgctca cccagcgaaa ggttgtattc gttgctgccg cgctcgtcgc aatggccttc
    12541 caccacgatg cccgcggtcg ggttctgctg caaatagccg accaccgcat tgatcttggc
    12601 ttgctccgag gggttcaact gtgtgctgtc atactcgaac agcaccgctt caaactgacc
    12661 ggccacttcc gcgccttccg tgcgggcggc caaaccagcc aagtccgcgg ccgtgccata
    12721 gatgggattg ccattcgcgt catagccaat caccacttgg tcgctgtagg gcgcatcggt
    12781 gcccaacggc ttctttttct tcttcatgca accggtcgtg ccgaccatgg ccacagccag
    12841 caacaccatc aaaacactca tccaacggcg tgtcattttc tcttgctcct ctttattatt
    12901 tttaaatcgg ggttggcggc aaacggggat caattgctcc atgcggggcc ggtccaatca
    12961 ccggtagtcg tcaacgcgat gggtttatca cccatctccg ccatgtagtc aagcaggtaa
    13021 aggctatagc tgtaattcac gccgcgcacc gctacgaggt gccggccatc gggcgcccag
    13081 gaggggtcct cataggaggc gtcaaagggg gtaatcaccc tttcctgcat gatattcggg
    13141 tccacgacgg caatctggaa cttgccgccg ctctgggttt ggtaggcaat cagcccgttc
    13201 tggccccagt ccggcgcgac gttttgcgag ccgctgcgcg tcagccgctg cggcgccccg
    13261 ccggaggccg aaatgatata aagctgcggc gagccgctcg cgttggagac atagacaatc
    13321 cgcgagccat ccggcgacca cgcgggcgat ccttcgtccg cccgcggggt gttggtcagc
    13381 cgcgtcagcc gcttgctggc gatgtccatc acgtacaaat cggggttgcc gtccttggat
    13441 agaatcaacg ccatggagcg cccgttgggc gagatggcgc cgccggaatt gacgccgggg
    13501 aagctggccg cgcgcgtccg gttgcccgtg gccatttcga tggtgtacac atccggatag
    13561 cgcttcagaa acgaggtata ggagatgaac cggttgtccg ggctccagcg cggcttgatg
    13621 gccacggact tgtcctgcgt gacttgccgg atatcttgca ggctggagtc cgccagatag
    13681 agctccttcg cgccgctgcg cgtgcccacc atgaccagct tggccaggaa aaagctgggc
    13741 ttgttcatca ctttgccgag caggtcatgc gccacttggt gggccagata gattgcttgg
    13801 ttcgccggct gtgtataggt ggcattcaaa taggcttggc cggccgggtt cacggcttgg
    13861 caggtgaaca ccaccgcacc gcccgcctcg gtcagcgtgc cgctcacgat gacgctgccc
    13921 cccccttttg gggccacggt cagccagccg gagcgggcaa tgtccagctc caacgtgcgc
    13981 cgcgccgttt gtgccgccgc gccagaaacc tgaatgcccg acaaatccac ccccacttta
    14041 ccgccagccg ctttgacgac gttaatatgt gacgactgtc cccatgccgt cagcacgccg
    14101 cacaacaatg ccaccaccaa ccaactctgt ttcttcacga tcaaatttct cctgggcaaa
    14161 agggtaacaa accacgcggg atgctagcgg acttccgggc ggatgcaatc ccagaatgtg
    14221 ccgggcgaca gtcgtgccgc taccggcggt tcagttgccg aacagcgaga gctgatcgcc
    14281 gtcaaacggc ctcaatttgc gtgcgcgcgg ctgggctaat ttgggctggc cggcttcgct
    14341 caattcctgc tcttcgaggt tggccaagat ttctttggcg cgggcgatga cgtccgccgg
    14401 caggcctgcc agccgggcca cctgaatgcc gtaggacttg tccgccgcgc ccggcacaat
    14461 cttgcgcaaa aacacaatgc ggtcatcctt ttcgctgacg agcacgttgt agttcttcac
    14521 gccgggcagg gtcagggcaa tgtcggtcaa ctcgtgatag tgcgtggcga acagggtctt
    14581 ggctttcacg ctggcctggt tatgcagatg ttccgccacc gaccaagcga tggaaatgcc
    14641 gtcaaaggtg ctcgtgccgc gcccgatttc atccagcaca atcagggagc gcggtgtggc
    14701 gttgttgaga atgttggcgg tttcctgcat ttcgaccatg aaggtggagc gcccgcgcgc
    14761 gatgtcatcg ccggcgccca cccgggtgaa gacgcgatcc accacgccga tttccgcggc
    14821 ggccgccggc acaaacgagc ccatttgcgc catgatcaca attagcgcca cttggcggat
    14881 atacgtggac ttgccggcca tgttggggcc ggtgatgatc agcaattggt tgtcgtggtt
    14941 gtccagcagc gtgtcgttgg gcacaaagcg gccggactcc ggcagcagct caatcactgg
    15001 gtggcggccc tcgcggataa ccaacgggcc agcggcggag atttgcgggc gcacgtagcg
    15061 gcaggtcaac gcgcgctcgg caaacgcggc cagcacgtcc acctcggcca cggcggcggc
    15121 cgtctgctgg atggcagcag tggccgccac gatctcctcg cgcagcgcgc agaatagctc
    15181 gtattccagc gcccgcgatt tgtcctccgc gccgagaatc ttgtgctcgt agtccttgag
    15241 ttccggcgtg atgaaccgct cgccgctgac gatggtttgc ttgcggatgt agtcctccgg
    15301 cacgcgtgac aaattggctt tggtgacttc gatgaaatag ccgaacacct tgttgaagcg
    15361 gattttgagc gaggtgattc ccgtgcgctc gatttcgcgc gcttgcagtt cggcaatcca
    15421 ttgtttgccg tcgtgcgcgg ccgcgcgcag ttcgtcgagt tccgcgtggt agcccttgcg
    15481 gatcacgccg ccttccttga tggacatggg cggctcatcc accagggcgc gcgccaccag
    15541 cgccacgagc tcatcttgcg gcacgatctg gccgcgcaat tcggccagcc gcccgccagg
    15601 ataggccgcc agtgcgtcgt ggagcgccgg cagtttctcc agcgaggccg ccagcgcgcg
    15661 cacatcgcgc ccgttgccgg tgccggagga gagccgcgcc agcagccgtt ccaggtcctt
    15721 gatggcgccc agcgtttgcc gcagcgcatc cagcgcgtgc cgggttttca gcagcgccgc
    15781 caccgcgtcg tggcgggcca cgatggccgg cacttgctgg agcgggcgca ccagccagtc
    15841 gcgcagccgg cgcgagccca tggcggtgcg cgtggtgtcc agcgccttca gcagggtggt
    15901 gggggcatcc gcgcgcggtg tgccgcgtgc gaccagcaaa tccaaattcg ccagcgtgga
    15961 ttcatccagc atcatcgcat ccgtggcggt gcggaaacgg atgcggcgga cgtgcgccgc
    16021 gttgcgccgc aagtcgcgcg tcacataata cagcagcgcg cccgcggcgg cggtggccgc
    16081 cgggtgcccc tcgccaccga aaccggccag cgattgcacg ccaaagtgcc ggcatagacc
    16141 gtcatcggct gtcgccggat cgaaaatcca gtcgtcgcgc gcggtcaggg caaaatcgcc
    16201 cgtggcgcgc agcgcgctca aaaacgcctc atccgcgtgc aggcgctcgg aaaggatgac
    16261 ctcgggcggc gcgtagcggg ccagatcatc cagcagcgcc gtcgggtccg cggcttcctc
    16321 catccagaac tcgccggtgg acaggtccag catggcgagg ccgtagagct tgtcctgccg
    16381 gtgcaggccg gccagatagt tgggccgcgc cgcatccagc atggaatccg tcagcaccgt
    16441 gcccggcgtg acgatcccgg tcacctcgcg cttgacgatg cccttggcca gcgccgggtc
    16501 ttccatctgg tcgcacagcg ccaccttttt gccggcgcga atcagcttgg ccagatatgc
    16561 gtccgccgcg tggtacggca cgccgcacat cggcactcct tgccgcttgg tcagcgcgat
    16621 ccccagaatt tccgccgcca cgatggcatc ctcgaagaac atctcgtaaa aatcgcccag
    16681 ccggaaaaac agaatggtgt cgggagcaat ttccgccttg gcgcgccggt attgcgccat
    16741 catgggggtt gtggattttt cgctcatcgt cgtggcgaca atgaagcaaa ccgtcgcgcg
    16801 gctgaacagc caaaacaccg cgcgccatcc gcaagaaaac cgcggcgcag ggttgccaag
    16861 acccgcgcgc ctctggtata acccgcgcca ttgttgacaa ggagtggctt acgtggcgaa
    16921 ggatagcaat ttaatcgtgg tgcccggcga ggtggtcaaa gtactgcccg ccacgatgta
    16981 ccgcatccgc ctggaaaacg ggcatgaggt tctcgggcat atttccggca aaatgcgccg
    17041 gcatttcatc aagatcacca ctggcgacaa ggttaccgtg gaaatcaatc ccacggacct
    17101 gaccaagggc cgcattacgt tccgcgaaaa gacctgagcg cgtgccgcgg ggcgcagggg
    17161 agttcagttc aaccgcaagt ggcgcagcca ctggcgaaaa tccgggggca atggggatac
    17221 cgccaccacc ggccggccgc cgaccggatg gctgaagcgc agctcgtggg cgtgcaacat
    17281 ctggcgcggc accgcgctga acgccgcgct gcgcgaaccg aaatactgcc ggtcgcccag
    17341 caccggcgtg cccacgtgct gcaaatgaat gcggatttgg tgcgtgcgcc cggtttcaat
    17401 cagcaccgtc acatgcgcgc agcgcggcgg cgcagattga aaagaaacct gccgcacgcg
    17461 actgaccgcc gtcaggttgt ccaccgcggc gcggatttcc atttgcggct ccggcaggtt
    17521 gccggcaatg agcgcgtggt acagcttgcg cacggtgccg gcggcaaatt gcgccaccag
    17581 cgcgcggcgg gtggcgtcat ccttggccag caacacgcag ccggaagtgt ctttgtcgag
    17641 ccggtgcacc gcgcgcaacg cgggcacgcc gagttgttcg cgcaggcgcg tctccagcga
    17701 acgctcgccg gtggtcagga gctggggtgg tttgtcggcc accacaaaca gggagtcatc
    17761 caccagcagg cggattggcg cggccggcgc agtgcgcacg gtggcggtgg gcagcaactc
    17821 cacaacatcg cgggtttgca gcaaatgccg cgccatccaa atgcggcggc cattgacgaa
    17881 gaccaaccgc tcgtccagca ggttcttggc cccgcgccgg gatagctcga ggcattcggc
    17941 cagatgatcc aatagcggcg tgccgttgcg gcgcacgggc ggcaaacggc ggagggtggg
    18001 ggtggggccg gcggatcgct tcattggctc ctccaacgga agggcgaaaa gttggtttgc
    18061 acgtcatacg gatcggattg ctccgcccgc ttcagccgac caatcaggaa atacgtcagc
    18121 ggcgtggcca ccacttcgta gccggttttg aggacgaagt tccaaaacga aacacgcgcc
    18181 acgagaccca gcggccacac gccgccaaac gcaatcgcgg tgaacagcag cgagtccacc
    18241 ccttcgcctg caatcgtgct gccgattgtc cgcatccaca ggtgccggcc ctgcatccac
    18301 accttcattt tggccatgat ccatgcattg aggaacgacc cgcaccaaaa cgccgccatg
    18361 ctccccagca ccacgcgcgg ggtctgccgc aaaatggccg taaatgccgc ttgcagctcc
    18421 cagccgggcg ccgccggcag gctggccacc agcacaaacg tggccgccgc gaacaggttc
    18481 aacacaaacc ccagccagat gatgcgccgt gcctgggcat agccatacac ctccaccagg
    18541 atgtcgccaa aaatatacga aatggggaac agcacgttgc cgccatccat caggacgaac
    18601 ggcaaaccgg gcagcgcaac cggcttgatg gcggcaatgt tggaaatcac cagcaccgta
    18661 cagaacaccg tggcgatggg cgccagccat cgccgcggtg ctgtcggcgc ctggacgggg
    18721 gtcatttctt gagcgggttg aggtgcttca acgtatcgcc cacggccttg cccgcatcca
    18781 ccgccgcccc gccaatggcc ttgccggcgt cggctgccac gccaccgacc gctttccccg
    18841 cgtccactgc cgcgcccccc acggccttgc cggtatccag cgccgcgccg ccgatggcct
    18901 gcacgccatc gcccagcaaa tgccccgcgc cgccaatggc cgaacccgcc gcgcccgcaa
    18961 tggcctgcaa gacgctttgg atggtctcga gcaacgtcgc gccatctttg tccttgccta
    19021 aatcggtcag ggtgatcggc ggcaacggaa tcggcaccgc gcccccgccg gtcagcgctg
    19081 ccgcgcccgt cagcgagaag ttcaaccggc tgccggtgat gctcaacttt tcaataatga
    19141 cctttttggc cggtttttct tctccggcct tttcttcagc ttcgggcttt tcttcggctt
    19201 ccttctcctc gtcggaaagc gattcgatca gcgcgccgag attgctgccg cgcaacccct
    19261 gctcataggt gaccaccagg ccgtcaatcg tgatttcctt gatgacgatg gtatcgctca
    19321 acagcgactt gttatccagc cgtaccgcca cggagcccag gtccagcagg ccgtcggtct
    19381 tgaagccctc cggattgccc acgtgcagcc cgcccagcgc cgccttgccg cggaagagcg
    19441 aaatatccgc cttggccaac gatactggca cgcccagcag ttgcggtccg gccgcgttgg
    19501 tggccttcag caccaccttg tccagcgaca gttccaaaac caccgccacg attgccagca
    19561 ccaccaaaat gccaatcagg attttccaca actttttcat tgttcctccg tctgcttcca
    19621 ttccgattgc cccccaccat accacgtccc cgcccccctg tcctaaaaat ccatacacga
    19681 caccgcagcg ggtgctgcgg gggaataggc ttgcagttgg gcgggaagcg gggagaatgc
    19741 gggcacgtga agagtggcga gtctacatat gccgtatccc attgaaaaaa agtttgtcgt
    19801 ggcggtgtca tccaccgtgc tgttcgattt gtcgtatgaa catgccctgt acatggagca
    19861 ggggctggag gcgttccggc gctaccagca cgagcaccgg gacgacctgc ccaagccggg
    19921 caccggcttt ccgttcatcc gccggctgct actgctgaac caaatgtatc cggaggagca
    19981 tccggtggat gtggtgattt tgtcgcgcta ccacgcggat gacgggatgc gggtgctggc
    20041 cgcggtgcgc gaatacgggc tggacatcac gcgcgcgttc ttcatggccg ggcgcaagcc
    20101 gtatccgttc atggagtcgg tcaatgccac gctttatttg agcaccaacg cggagcaggt
    20161 gcgcgaggcg gtggcgctgg gctaccccgc cggccacgtg ttgccgtgcg aggctgtgga
    20221 tgacgagcag gacgatcagt tgcgcatcgc cttcgacttt gatggcgtgc tggtggatga
    20281 cgaggccgag aaggtctata agcagacgca ggatttggac aagttccgcc aatatgagct
    20341 ggaaaaccgc gaccgcccgc tgaagtccgg gccgctgatg ccgctcttcc agcaactggc
    20401 ctatttccaa aaattggaca gccagcgggc acaggatgat tccgggcgca aacggctgct
    20461 gcggatttcc atcgtgaccg cccgcggcgc gccggcccat gaacggctca acaacacgct
    20521 gtcgtcactg gggctggagg cggacgaggt gttcctgatg gggggcatcg aaaagaaacg
    20581 ggtgctggat attctcaagc cgcacctgtt cttcgatgac cagttggcac acttgcaacc
    20641 ggtagcccgc agcacgccat cggtgcacat tccgttcggc atcgccaacc ggggcgggga
    20701 agattcttga caagaattag ccaattatgg tgattgtcca ctggtgaaaa ggatgtccaa
    20761 tgccaaattg cgggaggcat ttcatgccgc cggcatgcgg ctgacgcgcc agcgcctgct
    20821 gatttgccgc gagttggcca accgctatga ccaccccgat gtggagaaaa tccatcaagc
    20881 ggtccgcacg cgcctgaagc ggatttcgct cttcaccgtg taccgcacgg tgaacgtgct
    20941 ggagcaacac ggcttggctt ggcgggtcac cacgtggaag ggccacgccc gctatgacgg
    21001 caacacccac gcccacgccc acttcctgtg cgaagaatgc gggcgcattg atgacgtgga
    21061 tacgcaaggc gcctgccacg agatggccga gcgcgcccac gaatgcgtgc ccaaccgcta
    21121 ggtgcgccgc gtggatatga tgatcatcgg cacctgcgag gaatgcctcg ccaccaccgg
    21181 cgtgccgaca gatacgccct gagttccgcg ccccccgaaa atctcgttgt cactcgttgc
    21241 attgtggggc tggaacgatc acagaccttc cagccgttct cggcgggaag gtttcgtttt
    21301 ctcaaccatt cccgccctac aaaagcagcc ggttcgattg tagggcagga acggccgcag
    21361 gccttccagg cgttttcggc gggaaggttt cgttcctcaa ccattcccgc cctacaaaag
    21421 cagccggttc gattgtaggg caggaacggt cgcagacctt ccagtcgttc ttggcgagcg
    21481 ggtgccggcc ttggggcaac aaaaaagcca cccgtgaggg gtggcttggg tgccgttggg
    21541 gcggtgatta cttcgcggct tcgagcgcgg cgatcagcgt cggggcctgc tgcacgccga
    21601 cgaactgctt cacggcctgg ccatccttga agatgatcag ggtcgggatg ctctgcacgc
    21661 cgaattgcgc ggcgacggag ccggcatcgt ccacgttgag cttggcaatt ttggcgccag
    21721 ccactttgcc gacgacctgc tcgaggatcg gggtctgcat acggcagggg ccgcaccacg
    21781 gcgcccaaaa gtccaccaga atcaggccgg actgagtctc gcttttgaag ttttgttcgg
    21841 ttagttcaag tattcctgca ctcatgttgt tttcctttcg ttgcgggatt ttgttttttt
    21901 acaggggtgg ggttatttgt ccttcaggcc cagcttgcgg aacacgatgg agggcaggca
    21961 aaagccggtg aaggcggact gaatcaggtt caggccgata aagatggtga acagcagcca
    22021 ccacgggctg tgataatgga cgagggccac ggacaccaga atcatgatgc cggcaaagac
    22081 ccgaacggcg ttttcaatgg tcatgggagg tttccttttc agcagggggg gctccccgcc
    22141 ggcgagccgg cggggagcgt caagccgctt atgacttgtt cagcactttc ttcaggactt
    22201 cttccttcgg cagtttgcgc gagcagaagt accactcctg gcaaaccatg tcttccttgc
    22261 cgtccatgcg gttcttggcg gaactgcaac tggcggcggg gctgatgatg acgtcatcgc
    22321 cggggcgcca atcggcgggg gtggcgatgc cgaacgcatc ggcggtttgc agggcgatca
    22381 aggcccggta gagctcgtcg aagttgcggc cgaggctcag cgggtaatag atgatcgttc
    22441 ggatgatgcc cttggggtca atgaagaaca cggcgcgcac cgcctgcgtg gtggcttcgc
    22501 cgggttggat catgccgtag gccttggcga cgttcatggt gatgtcctcg atgagcggga
    22561 acttcacctc gacgttcttg tggccgcggt attcgatctt ttcctgaatg gagcgcagcc
    22621 aggcaatgtg gctgtagagt ccatccacag aaaggccgac gagcttgcag ttggcttccg
    22681 cgaactgctt ctccatggac gcgaaggtga tgaattccga cgtgcacacc ggcgtgaagt
    22741 ccgccgggtg gctgaacaga atcacccaac ttccctcgta ctgtttcggg aagtcaatgt
    22801 cgccttgcgt ggtcttggcc ttgaaggcgg gcgccttgtc gccgatgcgc ggcaacgaga
    22861 cgggagctgt gttttgctgt tccatgtttt tcctttcggt tgggggttcg gggttacttt
    22921 tacacagggg ttaacgcccg gacgcggtga ttgcccccac aataccccaa accagcatgg
    22981 aaatccacgg gttggagttg agggggcagg cgccggtttt gcagccgaca aagcggtaga
    23041 tggcaaagcc aatggccgcg ccgaccgctg cgccgaggat ggttttcaat aatgggctca
    23101 tggggtctcc tgatttttcg gggcaacacc gctggctaaa aaatggactc caccggcagc
    23161 gcaaagacgc ggaggccttc gccggagtag agggagtcgt ggaaggtctt cagcgcggcg
    23221 agcaacttgg cctttttgtc ttcgtcaatg gccacgaaga tcagcaccga ctgtcccgga
    23281 aacgtatggc tgccgaggtg cttgccggtt ttgccggaac cgaagacgtt ggcgatctcg
    23341 ctaaaggcgc cctcgccgtg gtcggcaatg aggttgcgga cttcgccgcg ccggttttcc
    23401 ggacaaatga tcatcagcat ttgcatggtt aggcctccgt tggttgcggt ttgcggtaga
    23461 tcaggccgta ggccacgggc accacgacca gcgtgaatgc ggtcgagaca aacagaccga
    23521 agatgatgga ccacgccagc ccgctgaaga tgggatcgaa cgtgatcggc caggcgccca
    23581 gcagcgtggt gccggcggtc aggaagatcg ggcggaagcg caccgcgccg gcttcgataa
    23641 tggcatcctg cagcgacttg ccgcgcgcga cttcgctgcg gatgaagtca atcaggatga
    23701 tcgcattgcg ttggacaatg ccgccgagcg caatcatgcc gatcatggcg gtggcggtga
    23761 agaagactga gtcggcatag ccgcccacgg tggcgccgcc cacggcgttg agcagccaga
    23821 agccgggcat gatgccgatc agcgtcagcg gaatggagag catgatgaca atcgggagca
    23881 ggtacgagcc ggtttcatag accagcagca catagatcgc cagcagcgcg gcggagaacg
    23941 caatgccgag gtcgcggaac acgtccacgg tgattttcca ttcgccttcg ccggtccaga
    24001 cggcgtgcgt gccctccggc aacggatttt tcttcaagtc ggcttgcaga ttcagcacgg
    24061 cgtaggccgg gccgcggcca gccatttcgg cggtgacgta caccacgcgc tccatgttct
    24121 tgtgcatgat ggtcggctcc accacgtttt cttcaaacgt gccgagttcg cccagttgca
    24181 cgctgttgcc catgcggccc ttgaccatga tggtcttgag gcgctccgga tcggaacgct
    24241 tctcacgcgg caggcgcagc acgatgggca gctcgttttg ctcgcgcggg gcgtgcaccg
    24301 tgccagcagg catgccgctc agcgcgatgc gcagcgtgtt gacaacatct tccgtggaaa
    24361 cgccgttgag accggccttc tcgcggtcca cgcggaagaa cagcttctgc tgggcggctt
    24421 cggcggagtc gtcaatgtcc accacgccct gctcggcggc catgcgcgtt tgcaggtctt
    24481 cggcggcggc gataagcgcg gagtagggct ggtcggggcg gccatagatt tccgccacca
    24541 cggtggagag taccggcggg ccggggggca cttcgacaat cttcaggttg gcgccggttt
    24601 gccggccgat ggcttcaatg tcgttgcgga tgcgcagggc gatggcgtgg ctgctcattt
    24661 cgcgctgctg gcgcggcagc aggttgatgc gcacatcggc catgttgggg ccctggcgca
    24721 gataatagtg gcgcaccagc ccgttgaaat ccatcggcga gccctcgccc acggtggaca
    24781 caaagttgac cacctcgggc acggtttcca gatagcgctc gatttcgcgc acggcggcat
    24841 cggtggcttc cagcgtggtg cccgcgggca tgtccaccac aatttgcatt tcgtccttgt
    24901 tgtcgaacgg cagcatcttg agcggcacgc gccccgaagc ggccaggaag atggagaagc
    24961 cgaacagccc ggccacaatc agcagcagca aggagctgcg gccgctggac gagacaaacg
    25021 gcgccatcag ccggtgatac acgcggtagg tgccggtggc ggtcacgtca taggccgcga
    25081 cctccggcgg ggcttctttg agcagcttgc tggaaatcca cggcgtgatg gccagcgcca
    25141 ccagcagcga ggacagcatg gtcagcggca cgttgagggc catgggcgcc atgtacgggc
    25201 ccatcatgcc ggtgatgaag aacatcggaa tgaacgagac aatgatcgaa agcgtcgcca
    25261 gcaaaatcgg cggcatgact tcctccatgg cgagggatac cgcgcgcagc cgcgggtatt
    25321 tcttcatgga gaggaaccgt gaaatatttt ccacgccgac gatcgggttg tccaccagca
    25381 gcccgagggc caggatcagc gcgaagagcg tcacgcggtt gatcgtgtag ccgagcagat
    25441 agttgaacag cagcgtcagc gaataggtga tgggaatggc gaggcccaca atgatgcctt
    25501 cgcgccagct catggatagc gcaatcagcc ccaccacggt caggatgccc atggcgaggt
    25561 tcatcacgag gtcgttgacc ttctcgttgg cggtgtggcc gtagtcgcgg gtgacggtgg
    25621 cttgcacgcc gtcgggcagg atgctgccgc gcaggtcgct aatcatccgc gacacctccc
    25681 gcgccacgtt gaccgcgttg ctgccttttt tcttcgccac ggccacggtc accgctggtt
    25741 cgctttgtcc aggcgccatg ccgttggcca catgggcggc ggcgggaccc gcaccgaggc
    25801 gggtgtagga attcaattca tccgagccat cctggatgtc ggccacgtcg cgcaaataga
    25861 ccggccggtt ttggtgcacg cccaccatca ggttgcggac ttcgtccacg ttctcgatga
    25921 acgggccggc ttccaccaca atggcccggt cggcctgctc gaaagtgcca ctggtcattt
    25981 gcgcgttgga aatcttaagc gcgccggcca cttccatggc cgagaggcca taggccgcca
    26041 gccgctcggt gtccagatac acatggacca cgcgcttctg gccgccatgg atgctgatgc
    26101 gcgcgctgtc gggaatggtc tgcaactttt gcgccacctc ctcggccagg cggtagagct
    26161 gatgtgtgtc cgcccccgcg ccgtgtagcg tcacgttgac gatgggcaca tcgtcaatct
    26221 cgatgggctt gaccacccag ccggcaatgc ccggcgtggt ttggtcaatg ttctggaaaa
    26281 tcttgttata gaccttgatc aggctgtctt cgcgattttc acccacgaaa aaacgcaccg
    26341 tcaccacggc catgccgggg cgcgacatgg aatagacgta ttccacgccg tcaatctggt
    26401 agagcagctt ttcgaggcgg gaggagacga gttgttcgac ttcctccgcg gagctgccgg
    26461 gcatcatgac catgacgtcg gccagcggca cgactatctg gggctcctct tcccgcgggg
    26521 tgatgaggag cgcggccgcc ccggccgcaa ggctgatcag aatcagcagg atcgagaggt
    26581 tccccttcag aaacgcctga aggattcccg aggtaatgga gtgtttgcgc tcttccatgg
    26641 gttactcctc acgcacgggg ttcaccacga tttggttgcc ggcccgcagg ccggagagaa
    26701 tctccacgcg tccgtcatgg gcggggccgg tcttgacgag gcggcgcagg gcgcggccgt
    26761 cacgggcttc ctgcacatag gtcagttggc cgatttgcac cacggcggaa gccggcacaa
    26821 agagccccgc gcgcggttcg gcggccaccc acagccggcc aaacgtgccc ggcagcacat
    26881 cgccattcac gccgtccaga tgcaccttga cgagctgggt gcggctggcg ctgtccactt
    26941 cgctgacgat ttccgccacc gtgcccttga ctacgcgggc cgggcgctcc agggccactt
    27001 ccacttcctg gccgagcgcc aagcggtcaa tcagccgcac cggcaccggc gcttcgaggc
    27061 gcatccgcgc cgggtcgtac accgccaaaa gcagcatgcc ggggttggcc agatcgccgg
    27121 cttcaatccg ccggtccgtc accacgccgt caatgggcga ttccacctgg gcgtaggtca
    27181 gcatgacctt cacttcctcc acttgcgcct gcgcagcggt gaacatggag cgggccgcgg
    27241 tcagcgcctg gtcggtggtg gcctgcttgt cgaacaactg ctgggcgcgt tcaaattccg
    27301 tcttggcctg cgccagttgg gcttccgctg ccgccagttt ttggcggata tcgcggtcat
    27361 ccagcgtcag gagcgtctgc ccctttttca cgcggtcgcc cgccgaggcc aacacctcgc
    27421 ggacataggc cgggatgcgc gcgctgagat tgattttctc ttcggaggcc gtcgtgccga
    27481 ccacttccac gcggacgggc atgttttcct ctttcagttc gatagtctcg gcatccgccg
    27541 gcaaagccag gcccggcagg acttccagct tggccggctt gaccttcttg gccagaaaac
    27601 cgcccgacca cgcaattact acggccaggc cgacaatgcc aacgatgggc ttgagatatt
    27661 tcttgagagt tgctgctgct ttcatgggtt tcctccggta gttcgtacaa taaggtttag
    27721 gggttggggg tggtttcggc cgcgggctcg ttgcccagca tgcctttggc ccgtttgaga
    27781 ttcgagaggg cggtcaggta gtcatactgc gcagccgcat agcgggtttg catggccgtg
    27841 acgcccactt gggtttgcag gagaatggca atgtctgccg cgccctgctg gtattgctct
    27901 tgcacaatgc gccgcgcttc ttccgcggtt tccaagctct tgcgggtcac ctccagccgc
    27961 tcccacgctt cctgcgagcc gaggaaggcc tgctgcaaat ccaaccgcaa attgagccgc
    28021 gccttggctt cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgagc
    28141 acgtcggttt tgaccgcgcc gccggcctcg aaacgctcgc gggccacgcg gaggctctcc
    28201 tcgatgctct gcaccgcctg ctcctgcacc gcggcaaaag cctgcgcctg caaaacgcca
    28261 taaaagccgc gcgtgacatc gtgcaccaac tggttgcggg tggcggcgaa ggcctcggcg
    28321 ctggcttggg agcccagccg cgccatctcg ttgccggccg cgcgttgcac gtcaaacaac
    28381 cgccattgcg ccgtgatgga gccgcgcagg ttgtcggtgt cctccgggtc gtttgggttg
    28441 aagccggggt ccatcatgtt catctgccgt tggttcagct ccatcatgaa ggtctgaatg
    28501 gggttgtcgc tcacggagta gttggccgcc agtccgaccg acggataata ataggatttg
    28561 gcctgtcgca gcatggcttg ggccgcatca attttggcgg tcgccgcctg cagggtgggg
    28621 gagttgtcca gcgcggcttg caccgcctgc tccaacgtga ccgcctgtgc cacaggcgcc
    28681 aacgccagcg ccgccgccac catcaaccct cggaataaca tcgtcttcat caagccactc
    28741 ctttcttcgc ccgcatgcag ttcaaaatgg tgagcgaccg ccggtcgccc acggaatacc
    28801 aaacttcctt gccgcgccgc tcgcgcgcta ccagccctgc ctgcagcatc ttgcgcaggt
    28861 ggttggagac caacgcctgc ggctggcgca gttgctgggt cagggtgtgc accggcaccg
    28921 ctttttcgcg gtccaaaatg tccacaatct tcaagcggta cgggtgggcc aacagtttga
    28981 gaaccttggc catgcgcgcc atgtccgcaa tcgaaaccaa cggtgggcag ggagtggaat
    29041 tcgttttcat gccgcccaac atatatcatt atggaaatat gtcaatagta aaatatgtaa
    29101 aaaaataaat gcgcgccagc ccgcgttttc agcgaagaaa aaagccccgg ctacggctgc
    29161 gccgtcagcc tgtggaacgt gtacagcgga gtggtgtcga aaaagtccgg ctccgccaac
    29221 tggtcctgca caaacgcggc gatggcttcc gtgggcgagg ccagcacctc atcatttccc
    29281 tgcgcgcccc gcggtacgac ggccaatccg gctcgcaacg cgcgctcgcg gaagttttgc
    29341 agattcaacc cgtataagcg cagcacgtca tccgccactt ccgcctggaa aaggaaatag
    29401 ccggggagca acgtgaacgt gtccaagtct tccggatttt cgtggcccac ggccaacaac
    29461 aggtgatctg ccacccggtg caggctggcc gcaaagcggc tggtgtcctt caagttgttg
    29521 accataagca cctgatagtc gccattggtc tcggccgggc gaaagaaaac ggtgcaggga
    29581 atgtccgcat cgtgccaggt gccggccaga tcgggagccg cgactttggt ttcatccgtc
    29641 aaccatggcg ccagcgccgg aaacatacag ctcgcggtca gtaggcaaag cgtcagcagg
    29701 gcaagcaaac ggcaattggt tttcatggca ttctccgatt catggttcat gggggtgtgg
    29761 gtgttgaggt tgcaatcggt gtggcccagc cggtggcgcg cgatacggcc tgatgccaac
    29821 cggccagcaa ttgttcgcgg cgcgcagccg gcatggcggg cgtgaagttg cgcgctgttg
    29881 gccggcgggc aaggtcggcc ggggtgtgcc agccaagcgc gatggccgcc aacgctgccg
    29941 cgccttgcgc cgtggtttcc atgcaggccg ggcgttcgat acggcacgat aaaacatccg
    30001 cctgaaattg catcaggaag gggttggccg aagccccgcc atccacgcgc agtaccgcca
    30061 gcggccggcc ggcatctttc tccatggcag tcacgacatc cgcgcactga taggcgatgg
    30121 cttccaacgc cgcgcgaatg atgtgcgggc ggccggtgcc gcgtgtcagc cccaccagcg
    30181 tgccacgcgc atacatgtcc caatacggcg cgcccaaccc cgcgaaggcc ggcaccagat
    30241 acactccgcc ggtgtctggc aacgcagccg caatggccgc cgactctgcc gcgttggcca
    30301 aaatgccgag ttcatcgcgc agccattgga gaaccgcgcc gccaatgaac acgctgcctt
    30361 ccaacgcata ggccggggcg ccgggcgcgg tttgcgcgga aagggtcgtc agcaggccgt
    30421 gccgcgattc caccgcctgt tcgccggtgt tcatcaggag gaagcagccc gtgccgtagg
    30481 tgtttttggc ttggcccggt tcaaagcacc cctgaccgaa tagtgcggcc tgctgatcgc
    30541 cggcaatgcc ggcaattgga atgttgtgcg cggcccaacc cggcagcgcg caagtggcgg
    30601 cgattccgga ggagggcagc acctcgggca gcatcgcggc ggggatatcg aaaagctcca
    30661 acagttcggg gtcccactgc agcgtatgga tattgaacaa caacgtgcgc gccgcgttgg
    30721 tgaagtcggt cacgtggcgg tggccgcccg aaagtttcca gatgagccaa gtgtcaatcg
    30781 tgccgcagag cagctcgccg cgttgcgccg cggcgcgcgc ccccgccaca tggtcgagaa
    30841 tccattgaat cttggtggcc gaaaaatacg catccagcac caggccggtt ttggcgcgaa
    30901 tggtctcggc cagtccttgt tcttgcagtg cggcacaagc ggcggctgtg cgccggcatt
    30961 gccagacgat cgcgttgtgg atgggcttcc cggtgcgccg gtcccacacc accacggttt
    31021 cgcgctggtt ggccacggcc agcgcggcca catccgccgg cgagcaaccc gcttgcgcca
    31081 gcgccgcttc catcacggca tactgcgatt gccagatgac gtccggatcg tgctccaccc
    31141 agcccggttg cggatagagc tgcgggaact cctgctgggc tgtggcgcaa ggctgcaaat
    31201 cgcggtcaaa caaaagcgcg cgcgagctgg tcgtgccttg gtcaagagcc agaatgaatg
    31261 tcctcatgcc cattctccgg tggtggcgca gccccactcc tcaactatca gccgaaaacc
    31321 ggcccctgac cagcaaaatc agcggcgccg gtcgtggggg agactcaagg cgagcgctgc
    31381 cgccaaaccg gcaccagagc caccgccagc aagcccgagc ccaccaacaa cacgccgagt
    31441 tgcgcgcttt cgcggaacac cagcagcgac accgccaccc ccagcggtat tttaagatta
    31501 ttcaacaccg ccagccgccc ggccgagacg cgtcgcgccc cctgattcca ccagaagaag
    31561 cacagaccgg aggcgacgat ccccagatag gccagtgtgg cccattgtgc cggggatgtg
    31621 gttggcgtat ggcgcaacca aggcagcgcc gccggagtca gcgccagcat tccgcccgca
    31681 tgcagccaaa aaaacagctt tcgctccacc accggttgcg gcaaccggcg cagcaccgcg
    31741 cgataggcca gttgcccagc ggcgaaacag aggttggaga gttgcaccaa gaggaagccg
    31801 cgccacggtg ccgccgtttc cagttcggtc catttcacca ctgctgcccc cgccaccgcc
    31861 aatgtcgcgg ccagccaatt gaacaagcgg aaccggcgcg atccggcatc cgccagcagc
    31921 gccaccaaga tcggggtggt gacggtgaac aaagccacct cgtgagactg caagaaacga
    31981 aatgcgctca ggtacgccca atacatcacg ccgaattgca cagcaccgat ggccaccaag
    32041 aaagccgcct gccgcgcggg tacccgccgt acaaacggca aaaacaccaa cgcggacagc
    32101 cccagccgca cccatcccac ccacgccgac ggcaaacccg ccaacgtgtt gccgatcaat
    32161 ccgaacgaaa acgcccagat tagcgaaaca aggaccaact gcatcatggg ccggccagta
    32221 tgatccgccc cgccccccga gcaaaagaca aaaacagcct ttttgtttct gcggctgcca
    32281 tcatatcagc gccgcattca aggggggggg ggcacgatct atccgcccgg cctcaacctt
    32341 gtcagccgta cgggtccgca acgagtgaat aaaaacacaa aatacatcac tttaaaaatg
    32401 cccagagttt ataaagtctg gacagtagct taaaaatgac tgtttaaaaa taaactaaaa
    32461 aatatacgta ataatttata tatgattggg atatgaatat tttaaaaaat gtccagagtt
    32521 tataaactgt ggacagttga gcaaaaatta aaaatatgtg acgcatggtg cataaatgag
    32581 ggtggtatga aaaaccccgc cccggagggg ggcggggtgg ggtgatgatg agaggttatt
    32641 ctgtggtttc gggggaggct tccggggcgg ctttggggcc gcggtgtttg tggccgcttt
    32701 cggagccggc gcccttggcg tgcgggaatt tcttgaaggg ctcgcgggtg tcggggcgtt
    32761 cgttgttcaa gatgcgctgc aactgggctt cgatgagctg gtcgcggtcg cctttgcagc
    32821 tctcgatttg agccttgagg gcggcgaggc gttcttcggc ttgggccagc cgcttttcct
    32881 tgctggcttg ggcgaggtcc acggcttcgc cgattttgtc gcgaagctgc gcaacgagtt
    32941 cagccttctt ggcttcgtca gtttcgaggc gggcggcttc gcctaggctg cgcatggctt
    33001 tgtgcaggcc gcgcattttt tgcatggttt cggcatcgag ggggccgccg gcgtagcggc
    33061 cggcttcggc ggggtcgttt tgtccccagc gcggctgttc ggtgccggcc atggggcagt
    33121 cgcccgcgcg ggcgcgcatc ttaccttcgc cgcggccgga gcctttttcg gcaaaggcta
    33181 cactggccac gagcagggcc agggaggtga ggatcagtgt ctttttcatg tggggtctcc
    33241 tgtgggttgg tttggtttct acatttccat ggtgaggaag aaatcatcgg tggcaatgga
    33301 cgcgagcagg tcttcgagtt ccgtgaactc ggcatccatg ggatcgagcc aggcggtatc
    33361 gagctcggtg tcgtcggcga gcgcggctcg ctgggcagta gctggcgagt ggcgcgcgat
    33421 gtggaagaag gtggcgccga caagaaggac cagtgcggcg gccgcggcga ggagcggttt
    33481 ccaagccaca gccagcagtg ggcggggagg gtgcaagcgc gcatcaatgc gggcggtcgc
    33541 gtcagcgagg gaagcgggca tggtgggggt gatttccggc gcagtgacgg cgcgggtaat
    33601 gtggcgcaac tggtcgcgca actcccgggc atgggccgag tcggccagcg cggcggcgag
    33661 gtgggcctgt tgacgcggcg taagttcacc agactgttcg agcaggagca gttgttcgag
    33721 ttgagcgggt ttcatggggc ggagtcctgt agttcgcggt aagccgggcc gagcgccgcg
    33781 cgcaggcggg tcagcgcgta ttgcatgcgg ccgagggccg tgttgagcga gcagcgttgc
    33841 agccgggcaa tttccttgaa gggcaagccg gcatccatgc ggagccaaaa gacttcgcgc
    33901 tgttcaaagg gcaggcggtt tacggcggca gtgatttgtt ggcccagatc gtggccggcc
    33961 acgacggcag cgggcgaaag gccggcggcc ggcagcagct cgccgagcgg agcggggctg
    34021 ccgggggggg cggcatctag cgattcctcg ggccgccgcc ggcgcgcggc atccacggcg
    34081 aggttgtgcg cgatgcgaaa caaccagcct ggcaaatttt tttgacggaa gaagttcatg
    34141 ttgcgaatgg cccgcaccca ggtttcctga aaccagtcgt tggcttcatc gggattggcg
    34201 gcgaagcgcg ataaaaaaga aaacagcggc cgctgatatt tggccacgag ccggccgaga
    34261 gcggcggtgt caccgcgacg atacgctgcc aattcttttg catcatccac catgttgatt
    34321 gcgtgttcac ctgaggtctt catgacagat aacgcatcag ggggcggatt tattgtgcgc
    34381 ggaaattatt tttccacggt gtggaaaaaa tatttccaca gtgtggaaaa attcgccggc
    34441 gcggggtgag cttttttcca cagtgtggaa aagttgtggc gccggcgggg tggggagttg
    34501 gccggcgggg ggaatcatgg cggggggacg gggatggcgg ccagcagggc ggccttgtcg
    34561 gtctggcctt cgagttgctg atcgtaggcg gcttgcagga tttcagaaaa gtgcgggccg
    34621 ggggtgtggc caagggcgat gaggtcgcgc ccggtgacga gcggcggggg gaggacgggt
    34681 tcggcggcaa actgggcgtg agcgttttgt aaaaagtggt agatatccag ttgagcgtgg
    34741 gaggagatgc agtcgaggcg gtgcaactcg agttcgtcgg cgaagagtgg ggagcccagc
    34801 aggcggcgga gtttggcggg gcgcatattg ggggcatcgg cgaggcgcat gtggccggcg
    34861 atgatggtgg cgacttcctc cgtgagcgcg ttgggcgcgc gcaggcgggt gaggattgcg
    34921 cgagccatgt ccgcaccaac gctggcatga ctttcgaagc gccagcgttg ggtgccatcg
    34981 cgcagggtgg cgtagtgggc ggtgggcggt ttgccgacat cgtgcagcag aacggccaaa
    35041 gcgaggcgca gagaggggcg gggcgggagc gcatcgagca tgaggcgggt atgggtgaag
    35101 acatcgccct cgggatgaaa ttccggcggc tgttccacgc cgatcatggc ggcgacttcg
    35161 gggagaattt cgcggagcaa gccggtatcg tgcagcagtt ggagggcggc gccggcttgc
    35221 tgggattcgg tcagcaggcg gaagagttca tcgcggatgc gttcggcgct gatgcggtgg
    35281 atttgagagg ccagcggcgg aatagccgca cgggtggcgg ggtcgagggc aaaatcgagc
    35341 gtagcggcga agcgcaccgc gcgcagcagg cgcaggtggt cttcctgaaa gcgggcggtt
    35401 ggatcgccga tggcgcggat gaggcgcgcg gcgagatcgg cctggccgcc gacgtagtcg
    35461 cgcaattcgc cgctgacggg atcgaggagt agggcgttga tggtgaagtc gcggcgttgg
    35521 gcgtcggtgc gggcatcgga gaacgtgacg gcagtggggt gccggccatc ggtgtaggga
    35581 gcatcggcgc gataggtggc gacttcgacg ggcggacctt cttcggggac aacggtgatg
    35641 atgccgaacg ctttgccgat ggcgtgggtt ttgggaaaca gcgccatgat gcggtcgggg
    35701 agggcatcgg tgacgatatc ccaatccttg ggcgggcggt taagcaggag atcgcggacg
    35761 cacccgccga cgaggaacgc ttcgtggccg tgctggcgga gggtcaccag aatttggcgg
    35821 ggggcgggcg ggatggcgtc ggcaaacttc aagcgcgcaa atccgcgtag agcgggcaat
    35881 ggtcggaggg tttggggcga gtgcggggag tcaggtcaat ggcggcggcg tgggatttcg
    35941 cggcgagggg gggcgtggcc aagacataat caatgcgcca accacgtttt tgttcggggt
    36001 tgaagggcac gcggtaatca tagaacgaat aatccaccag atcgggatta tgccggcgga
    36061 agagatcggt gaacccgagc gcgagcacat cctcgaacgc ctcgcggatg gcgtggtgat
    36121 agcagacgtg atccttcttg gtttccgggt tgctgacatc tatcgggtgg cgggccacgt
    36181 tcaggtcgcc gcaccaaagg agggggcggg tgggtgcttg gcggcgctgg aaatactgtt
    36241 tgaggcgggc gagccactcc agtttatagg cgtacatttc gtgttcgatg tcgcgccctt
    36301 gcgggacata ggtgttcacc acgtggacct cgccaaaacg ggcgtggatg aggcgggtgg
    36361 ggtcggcggg ttcgctatcg aggccgaagg aaacctcgtc ggcgggttgc ttggagacga
    36421 tggccacgcc gttataggat ttttcgccct tgaaggccac gtgccagccg gcggcggtga
    36481 aggcatcggt ggggaacagg tcgtcagtca ccttggtctc ctggaggcag aggacatcgg
    36541 gggtgtagtc gctcaaccag cgcaagacgg cgtcgaggcg ggagcggacg gaattacagt
    36601 tgaatgtggc aatgcgcatg atgcgggtca ttctaccacg cgcgcggcgg catcgcctct
    36661 gtttttttgg gcggggatca ggcgcgggcg gcgcgcggga gatacttgaa gtagagcgcc
    36721 aaatcggtgg ccaccagcag caggttgagg gtgtagagcg cgatgaccgg atcgcgggag
    36781 tagagccatt tgtgcgcgat gccggacgcg tagccaaggc acaaaatggc catgaacagc
    36841 gggcttttgc ccacgacaac tttcgtgcgg atggacttgg cgatggagat gggccaactg
    36901 atgccgaagc agatcaacat gcccgcttca aatatgctca tgagtcacct gtggggatgg
    36961 tgttaggggc ggagggcgtt gaacaacgag gcccaagcat cgcgttcggg aaaaggggcg
    37021 tagcgggttt gcagggccgc ggcgatggcg ggcgcggcgg cgggcaaatc gagaaagcca
    37081 ccgagggggg cgctaccttc ggtggtgtca cgccaaagca cgaggcggac gggttggccg
    37141 gcgatcgtgc cgatttggta cggggtttcg aggaccatgg tgggcagggg ggcgggcgcg
    37201 acggcgcagg ctgtgggcac tacgttggtg tgcgcatcgg gcgcggcatg cggctcggtg
    37261 tggacggttt tccacgcgct ggcggttgtg cggcagcggc gaatccacgt gccgatgtcg
    37321 ccgggggcga gttgatccgg gagcatctca ccacgccaga acagcacgcc gtgtttttgt
    37381 tggatgcggc catcaggggt gtcgaacgtc agaatgacct gcggcgggag ggggcgggag
    37441 tcgggtgtga agaccacgcc ggcggttgtg gcggccgccg cgatgtcctg ctcgaccgcg
    37501 gcaaagattt cagcgccgcc gagcgtgacg gggtccagcg gcaaacattc cgcgcgggcg
    37561 ggggggaggg tcaggaaaag aaccagcaat agcagccaat aacaccgcat ggttattcag
    37621 aggaacgatg gcgggacagg tgcaggaact cgttgcgcgt ggccacggag tcgtggaagc
    37681 tgccaagcat ggcgctggtg accatgcgcg agccttgttt ttccacgccg cgcatcatca
    37741 tgcacaggtg ctgggcttcg atgatgacgc cgaccccctc cgggcgcagg gtatccatga
    37801 tggtgcgcgc gatttggttg gtgagccgct cctgcacttg caggcggcgg gcatacagat
    37861 cggcaaggcg ggcgagtttg ctgaccccca acaccttgcc gcgcgggata taaccgatgt
    37921 ggcagacgcc aaaaaagggc aggagatgat gctcgcacag cgagtagatt tcgatgtcgc
    37981 gcacaatcac catgtgattg gcctcgacct cgaaaatggc ggattgcaac aaggcggcgg
    38041 ggtcttggcg gtagccacgg gtgagatagc cccacgcctc gtccgcgcgt tggggggtgg
    38101 cgagcagacc ctcgcgcgtc gggtcttcgc ccatcgcggc gaggatttgt cggaattggt
    38161 cttgcatggg gcggattaag ccacaagccg cccaagcggg caatcccgga aagacaaagc
    38221 gtggattact ccaacggtaa tttggggccg gccggcgaac cggcggggcg catggccgcg
    38281 cgtgcctgca cctgcttcaa cgaattgatg aacgccgccg ggccaccggg ttggtagccc
    38341 aactgaccga tcagttccgc agcggagttg aacacaagaa tggtcgggaa cccttcaaca
    38401 cgaaaggcgc gcatgagccg ctcattttgg gcctgcaggg cgggcggcaa cttggtgccg
    38461 cgtgggaagt cggcgatcac gcaaaccaga ttggccgcgg catagtcctt gaattcggaa
    38521 gtcgaaaaca cttccgcatc cagccgcttg caccagccgc accagtcgga gccgctaaaa
    38581 ttcagcaaaa tcaacttctg cccggtttgg gcatcgctgg cggcggcggc caaatcggta
    38641 cgccattcaa aggcgttgca ggacgacagg ggcatgacaa aaatcaatgc ggccaacagc
    38701 agccacggga tggatttctt gatgttcata ttgatttatc cttgttcaat catagaaagt
    38761 taccacacgt tgcgccttgg cgcaaacggg cttggcggtc tcgtgggggt gcggtagcat
    38821 cctttttcat gtttttggga ttgctccaga tgttgtcaaa agtagtggcg gccctgccga
    38881 aacgcggggc ggaggccatc gcgcggcatt ggggctggct gctggcgcat gtgattcgcc
    38941 tgcgccggcg gtacgtgctg gccacgctgg cccggagctt tcccgaaaaa tcggcggcgg
    39001 agtgccgggc catttataat gaagtgtgcc gtcagcaggc gttgaacgcc atggaactgc
    39061 tgcgctatgc cggcggccgc caagcggagt tggccgcaca gtttgaggcg gtggggctgg
    39121 aaaacctggc tcgcgcgcaa acggcggaat tgggcgcgtt ggtgttgatg gggcattttg
    39181 gcaattacgc gttgctggcg ctgcaagtgc cgcggctgtt caacctgccg ctggccattg
    39241 tggccaagcc gctgcgcaac gccacactca acgaccagtg gtgggaactg atgcgcaagg
    39301 cgggcatcac cggcattccg gcgcgcaatg cctaccgggc cagcgtgcgc gcgctcaaag
    39361 agaagaaact ggttgggttc atgctggacc aaaatcgccc ggcgacccag ggagtgtttg
    39421 ttgatttctt cggcaagccg gccgccacca cgcctggctt ggcgttgatg tcggctcaaa
    39481 ccggcgccgc agtgctgccg gcgtttgtgc accgcctgcc ggacggtcgg caccgggtgg
    39541 tggtccatga acccatcgcg ccgccgccgg accggcggcc ggaaaccatt cgtgagtata
    39601 ccgcccgcta cacgcggatt ctcgaagatg aaatccgccg cgaaccggcg caatggctgt
    39661 ggtggcataa gcgttggaag agtcgcccac cagcggagca gccccccgtc gccccatgag
    39721 caacgcgcgc accaagaatg ccgaaccgct ctggcggcgg ttacgcatga cgctgctgga
    39781 agtgccggcc acccggttgg cgttggccat cgtgccgcgg ttgccgcgcg gtgcggtgtt
    39841 gggcttggcg cggttcgccg ccaacgcggg ctatgcgtgc tcgcccgcca tgcgccgtct
    39901 gggcctgacg aatctggacc tggcgtttgg cgataccaaa acgccggcgg aaaagcggcg
    39961 cattttgcgg gagtccatgc gcaacttcat gctggtgctg ctggacacga tttggttttc
    40021 gcgcgattcc gtcgcgcggc tgcagcgctg gttccgcgcg gcccccagtt tgcaggcggt
    40081 catggcccaa cgggaagcca cggtgggcat cacggggcac ttcggcaact gggaattgac
    40141 cgggcgctac tgggccgagc aaaccggcgg gctgatgagc gtagccatgc cgctgaaaaa
    40201 tccggcggtg gacaaactgg tgacgcgcgt gcgccaactc aacaaccaga tcgttgtccc
    40261 acgggatggc gcgctgcgca aactggtcgg ctttttgcgc gccggcggca ccatcgggct
    40321 gctgctcgac caaaataccc cgccgcgcga gggcgggatt ttcgccaatt ttttcggcaa
    40381 gccggtggcc gtgtcgcccg ccgcgggcgt gttggccacg atgacccgcg cccggatttt
    40441 gtttgggtac accttgccgc agcccgacgg cacctatctg gccgaagcgc cgcgcgccat
    40501 cgccgccgcg gaaatcagcg ccatggaccg caaaaccgcc accgccgact tgacgcaacg
    40561 catcaacgat ttttacgagg aggccatccg cgcgcatccg gagtgctggc tgtggaccta
    40621 tcgccgctgg cggtacatgc cgcccggtgc cgtgcgcgaa gacttcccgt attacgccaa
    40681 atagaaagga tcctccatgc tcaattttgc cacctatcaa caacaagcga tgaccaccgc
    40741 cgtttatccc ggtcggggtg aaggcaactt tacgtatccc gcgctgggcc tggccggcga
    40801 aaccggcgag gtgtgcgaga aaatcaagaa ggccattcgc gatgatggcg gcgtcatttc
    40861 cgccgagcgg cgcgcgctgt tggccaagga aatcggcgat gtgctgtggt atctcgccac
    40921 gttgtccacc gagctggggc tggaactgca agaaattgcc gagcaaaatc tggccaagct
    40981 ggccgcgcgc aaagccaacg gccacattca tggcagcgga gatctgcgct aacatggccc
    41041 gcccggcagc cgtcattttt gatttcgatg gcgtgatcgt ggacagcgaa cggctgcacc
    41101 acgaaaccat ggtggcagcc atggccggcc aaggtcccgc cacgccgtgg gagttttatc
    41161 acgaacacct gatgggcttt gatgatcgcg gcgcgttcgc ggtgttgctc gaacgcgcgg
    41221 gcatcgcccc cacgcccgaa gaaatccagc gccgcattgc ggtgaaggcg cggctgtttg
    41281 ccgaagccgc ggcggcgggg cgagtgcccg cgttgccggg cgcggtggaa ctcgtgcgcg
    41341 cgtgcgcggt gcaagggccg gtggggttgt gcagcggcgc gttgcgcagc gacatcctgc
    41401 ccgtgctcga tgcgctgggc attgccgact gctttgcgga gatggtcact gcggatgatg
    41461 tgccgctgag caagcccgac ccggcttgct accggctctg cgtggcgcgc ttggccgcgc
    41521 gctttccgga agaaggcatc acgccggcga cttgcgtggc gattgaggac accacggatg
    41581 gcatcagcgc cgcgcgcggc gcaggcatcc cggtggtggg cgtggccacg aacatggaac
    41641 ccgcgttgct gctggcggcc ggtgcgcgcc gcgtctttgt ggcgctcacc gccattacgc
    41701 cggagcaact ggctggcggg tgatccaact tttccatgct gtggaaaaaa gttttccaca
    41761 ctgtggaaaa gaggattcag ggttcggggt tcaggatttg atgggattgt agggctggaa
    41821 cggtcgtaga ccttccagtc tttcgcggcg ggaaggtttc gttcctcaac cattcccgcc
    41881 ctacaaaaac agccatagcc gcatctgggt tggattgttg cggcaacctt ttcgcgggtt
    41941 ttccacgctg tggaaaaatc agcgtcaaaa gttccacacc gtggaaaaac aaggcgtttt
    42001 tgttccacac aatggaaaaa ctttcgccag attttccacg caatggaaca actttgcaga
    42061 gatttttcca caccgtggaa aaatggggcg cgaaagttcc acgctgtgga aattaatttt
    42121 ccacactgtg gaaaagtcgt ggcggcgacg gtgcgccgcg gcaatcaggt gggttttttg
    42181 atggtgattt tgatagcggg gcggccggaa ggtttgctgg cggccggggg ggcggcggcc
    42241 ggtttgcttt ccgccggggc agggggcttg gtctgggccg cgccttcggt ggggacgatg
    42301 tcttccacga tttccttgag gatggtggtg tagcccttgc catcagccat ggcatccttc
    42361 acggcttcga ggcccttttg ggcatcttcg gcggtggagg cggcttccag cacaaagatc
    42421 gtggcgccga tttgaatgcg gtcgccggct ttgattttga cggtgtcttc cacgcgctgg
    42481 ccgttgacat aggtgccgtt gcgggacttg aggtccttca agtaaaactc gccgtcggag
    42541 aggcggatgc cggcgtggat gcgcgagact ttgtcgtcga gcagggggat gtcggcttcg
    42601 cgggcgcggc cgatggcgag cggctcgccg gtgagtttga tgtctttggg aatgccgagt
    42661 ttgttgacgt agcgcagatg catgggggcc tccgggttta gacgattcct ttttggatca
    42721 gttcggcaaa ataggcgatg gtgagccgca ggccttcctc gagcggcatc ttgggttccc
    42781 agccgaggag cgcgcgggcc ttgctgatgt tgggctggcg caccttgggg tcatccaccg
    42841 gcagcggctt gtgcacgatg ggtgcggcag agccggtgag ttccttgatt ttgacggcga
    42901 aatccatgac ggacaactcg cgcgggttgc caatgttgac cggctcggcg tagtcgctca
    42961 tcatcaggcg gaagatgccg tcaatgaggt cggagacgta gcagaaggag cgggtttggg
    43021 agccatcgcc gaagacggtg atgggttcgc ccttgagggc ttggcagcag aacgcgggga
    43081 cgatgcggcc atccttgggg cgcatgcggg ggccgtaggt gttgaagatg cgcacgatgc
    43141 gggtggccac gccgtgcgtg cggtggtagg ccatcacaat ggcttcggcg aagcgcttgg
    43201 cttcgtcata gaccccgcgc gggccgatgg ggttgacgtt gccccaatag ctttccacct
    43261 gcgggtggac gagcgggtcg ccgtacacct cggacgtgga ggccaacagc aggcgcgcgc
    43321 ccttggcttt ggccagcccc agcgcgttgt gcgtgccgag cgcgccgact ttcagggtct
    43381 ggatcggcag ctccagataa tcaatcgggc tcgcgggcga ggcaaaatgg aaaatatagt
    43441 ccagcggccc ggaccagttg atgtgggtgg tgacgttgtg gtggatgaac tcgaaatccg
    43501 gccgcgagcg caaatgggcg atgttgtcga gattgccggt gatgaggttg tccatcgcaa
    43561 caacgcgatg cccctcggcg aggagacggt cgcacaggtg cgagccgagg aagccggcgc
    43621 cgccggtgac cagagtgatt gggcgttcgg atttcatgcc cccacaagat gccagatcat
    43681 tccggcttga gcaaggcagg atgcaactgg ctggcgaagg tggcgatggc ctgcaaatga
    43741 ttttcgttgg cggggttgcg cgggccggaa agggcgatat aggcccagcg gtgggaaacg
    43801 ccgtgcacca cgcggtcgta gcccatccag aacattcgct tcaggtgcga ggcggtttcg
    43861 cggcccttgc caacaaacca atacgcgtaa taagtgttga ggtcggcagg gttgccgttt
    43921 tcgtcccggt aggagcgggt catttccaac accatgattt ccagcggctc ggcgcggttc
    43981 ggcaggggca catggatcag gcgcgaggac acaatttcgt ggccggcggc ggtcatgcag
    44041 acctgcgggc ggtggatgct ggagcggtcg tcgccgctca gcacaatggt ggcgtgcacg
    44101 ccggaggtgc cgttgggcag tgaataatat ttgcgcacga ggccggtatc cgccggcaac
    44161 atggagcgtt ccgcccagtt catgttgctg aggggggcgc cgcagttggg gcactgggcg
    44221 ttgtctggca cggcgttggc ggcatattgc cggttgcagt tgcggtccgt gcagaagagc
    44281 aactccacgc cttcccacgg gcccacccgg gcgggcagcg ccagaacaat gccggcttca
    44341 tccgtggagg ccacgccggt cttgtacacc agcgcccagg ccgctacggc cagcgccgca
    44401 attacgaaga ggcgcggtcc aaaaaacgat ttttgcagat tgtccatgcg cggcggtagt
    44461 ccatgttgag caggcggtcg agggtcagca tcaggatgat gctgatgata aaaatcggat
    44521 agccggaata atcgtgccac aggcccatgg cggtttcctg tccccagaag gcggccacca
    44581 ccaccaccag aatcacccgg caaatattgg ctacgatggc caccgggatg cttaggaaaa
    44641 acagcagcca ttttttggcg agcgtttttt gcgaatacca ggcgtaaaaa cccatcagcg
    44701 ccgtcatggc cagcagcgag cgcaggccgc tgcactccgg cgccacgttg aaggcgaagg
    44761 aattgcccgc gcctgaaaac agccccgcgc ccacccgctg cacttccaac ccgaagccgt
    44821 tcaggatgcc acccgccacc attgtggcaa acaaccgcag cggcgcggtc atgctgtcaa
    44881 tgaagttgag tggaatggcc aaaaacaggt aggcgcacgg gaacagcagc cagcgggcca
    44941 gctcccaccc atatataagg aaagggaggc tccacagcaa aatgatgagg ccgccggcgg
    45001 aaagacgcgg ctgttgcgcc agcacgccca cccaatggat cgccaacgcg cccacgatca
    45061 gcagcgcgcc ccaccagttg gcgcgacgcg gcgcggccgc aatctcgcgc cgccggatcc
    45121 acagggccag caggctggcc agcggcagca ccatgcaata ggcatagtcg cccacccagc
    45181 ggctgttcag ccacaaaaag agcgatcgcc ggcacgtggc cacttcttgg ctgttgccgt
    45241 gcaggtggaa caggaaaaac agccccgcca ccaccaccgt ccaaaacccg atctccagtt
    45301 gcgcggcgcg cgtcagccgg gtccaccgga agcccgccca ccacgctttg agttgttgtc
    45361 cctggttttg catgagattg cccggcaacc taccggatgc gccgccggga tgcaaaacca
    45421 aagtccgggt tgaatcggcg tggggtcttg gcctatcctg cctgccatgc gaggtgatcc
    45481 catgaccaaa cccaccggaa aaatttggct tctgttgact ctgctggctc tggccgcacc
    45541 gttgggccgg gcggaactgg tccgtgaaga actgccggac gccagcgatt gggtgcacga
    45601 cattgtgttc gaacatgagg ccggcgcgga cgatggcacg ctgtccgtgc ggctgggcac
    45661 ggacccggtc accgtgcgct atttcgaggt gcccttgagc gtttgggccg agttcaaggc
    45721 ggcggaatcc aagggatcct tttacgggct gaacatccgc cagcgctatg aacggcaata
    45781 tggcaagtcg cgccaagagg tgttcgactc cccgctgccc atccaaacgc agatcaacgc
    45841 attgtgcgcc ttcaacgagg agtgcgagga cgtcgtgctg gaggggattg aaaaaagccg
    45901 cgaaagcatt tgggtggccg cctatgcctt cacccgcact aggattgccg ccgcgctaat
    45961 ccgcgcgcat cagcgtggcg tgcgcgtggc ggtgaagatg gatgtcaacc aggccgagtt
    46021 tgccggcgcc caaaaattga tcgagtggct ccgccgcgaa ggcatccccg tcacgctgat
    46081 ccgcaccgcc ggcgactact ctgccatgca caacaagttc atgattttcg acatgcgctg
    46141 ggtcgtcacc ggcagctaca acttcaccac caccgc
//
LOCUS       DAOPNP010000007        36847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1521_length_36847_cov_9.252691, whole genome shotgun sequence.
ACCESSION   DAOPNP010000007 DAOPNP010000000
VERSION     DAOPNP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 36847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36847
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_36847_cov_9.252691"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3308)
                     /gene="nifJ"
                     /locus_tag="PKI64_01495"
     CDS             complement(<1..3308)
                     /gene="nifJ"
                     /locus_tag="PKI64_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOD99713.1"
                     /translation="MSKKKQIAIIDGNEAAAHIAYKTTEIAAIYPITPSSNMGEWADE
                     WMSEGKTNIWGAVPTVVEMQSEGGAAGAVHGALQTGALTTTFTASQGLLLMIPNMYKI
                     AGELTSTVFHVSARSLAASALSIFGDHQDVMACRQIGWAMLASNSVQEVLDTALIAQA
                     ATLKARVPFLHFFDGFRTSHEISKIEMISDDVIRAMVSDQLVADHRARALNPEKPFIR
                     GTAQNPDVYFQGREAANKFYEACPDIVAATMDEFAKLTGRQYRPFDYIGAPDAERVIV
                     LMGSGAETALETIEVLQKQGEKVGAIVVRLYRPFAAKYFFAALPKSVKAIGVLDRTKE
                     PGSDGEPLYKDVLSALAQGVANGSFPTMPKVIGGRYGLSSKEFTPAMVKAVFDELKKD
                     APKNGFTVGIVDDVTHTHLDIDTTFFIRHPEVTNAMFFGLGADGTVGANKNSIKIIGE
                     ETDFYAQGYFVYDSKKSGSRTISHLRFGPNPIHSPYLIQKASFVACHQYEFLLQTDIL
                     RYASTGSTFLLNSPFSKEEVWDKLPKKVQARIIEKKIKFYNIDAYTVAERTGMGTRVN
                     TIMQTCFFAISGVLPKEEAIAKIKDSIQKTYGRKGDDIVQQNFKAVDETLANLYEIPY
                     SAVTSTIEIPPTVSPQAPEFVKEVIATIMVGDGDSLPVSKMPIDGTFPSATTRWEKRN
                     VSLSVPIWDENECIQCGTCSFVCPHACIRANEYDKSLLDKAPAGFKHAPVRNKPDKAY
                     TLQIYVEDCTGCGVCVASCPKKNKNDPTRKSINLGPKLPIVAQERENIKFFETLPLID
                     RAKLNLNMVKDAQYTLPLFEFSGACAGCGETPYLSLLSKLFGDRAVVANATGCSSIYG
                     GNLPTTPWAQNRDGRGPVWSNSLFEDNAEFGLGFRLTSDQQKLYAEQLVATLKDDLGA
                     ELADGILQADQKDEAGIAAQRARVAELKKKLAGNTKPEAFNLLAVADFLIQRSVWIVG
                     GDGWAYDIGYGGLDHVLASGANVNILVLDTEVYSNTGGQASKSTPRGAVAKFAAGGKP
                     VAKKDLAMIAMSYENVYVARVAFGANPPQAIKAFKEAEAHDGPSIIIAYSHCIAHGMR
                     EMRDGLEQQKLAVNS"
     gene            3796..4560
                     /locus_tag="PKI64_01500"
     CDS             3796..4560
                     /locus_tag="PKI64_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOD99714.1"
                     /translation="MPCLDIRAGRVVKGIQFDDLIDAGDPVAKAVGYEADGADELGFL
                     DISVAMDGTKPNFALLREVTSAVKIPVTMGGGIRSREDVAAALAAGAAKVSLATAAFR
                     QPDLVGHLVAEFGSNTIVVALDAQFNPKMPSEREVYVGGGKIPTGADAVEFARRMADA
                     GAGSFLPTSIPADGSKQGYDVELIRAIADATRRPVIASGGAGLLVHFREAVRQGHAST
                     LLLAGALHFGVFNIPQIKTYLAAERIAVRNPPTPGV"
     gene            complement(4557..>6731)
                     /locus_tag="PKI64_01505"
     CDS             complement(4557..>6731)
                     /locus_tag="PKI64_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99715.1"
                     /translation="ETAWENFVHPEDRPAAKAYLANYIANPQGFYDLRFRMRRKDGSW
                     AWIWSRGRLTGGTAQQPASLIGTHIDITENIRAVEALRSSEQKYRMLTESMRDVVWIA
                     DADTLRFLYVSPTVKRVRGYTPEEVMAGTLVDSLPPEEAHERRSLLKKALARFRSGEL
                     TENDFFVVELSPRCKDGSRITVETVSRLWHNPSTGKVELHGVSRDITERKQTEQERER
                     LMRAIEQTDESVVITDAAGMIVFVNPAFTRTTGYTREEVLGHTPSILASDVQPPEFFQ
                     NLWSVLAAGQTWTGRFVNRHKSGSIYTEQATISPVRDTTGRIVNYVAAARDITAQIRA
                     ENEKAALQAQLDHAQKLETLGRLAGGVAHDFNNMLQAILGYTELALEQTAAGQPIHSD
                     LREIQKVATRSTTLIRQLQTFARKQPAALQTLEVNPTLDNMLDMLRRLVGEGIQLQWH
                     PGENVGWIHVDPSQLDQIIVNLCLNARDAVGPNGCITLATAVMEIVRDGPATPCDLPP
                     GSYVLLTVRDNGAGMPPDVVEHVFEPFYTTKPVGQGSGLGLSTVYGIVKRHNGCIQVH
                     SVVGQGSEFRILLPRLASGIPPTEATATEWVELQAPNHEIILVVEDEPAILRVTRSML
                     ESLGYVVLTASSANEAAKLCRGKDCNIDLLLTDINLPPSNGIALSQEIKKRFPCARHL
                     FMSGYPANLDGPDAIDPAHFLAKPFTRLQLAQKVRAVLLQDR"
     assembly_gap    6732..6831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6832..8575)
                     /locus_tag="PKI64_01510"
     CDS             complement(<6832..8575)
                     /locus_tag="PKI64_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOD99716.1"
                     /translation="MNEAPLRLGWGAFWIALAAMAAGVVFIGWAVWAKDQWLREHLLG
                     QAHQLGQTISASNLQKLTGQPGDIGRPEYRRLKSQLMAAQQINPLWQWVYLLGRNADG
                     EFYFQLDSVAADTAEVSPPGQLYPEAPPQLRELYEQGLANTGGPARDRWGTWVSALVP
                     IKDPRTGKMISALGIDVPADRWLRLCARAAVIPALFWLALVLQGVVGVALLRRRANGP
                     LARQRRLRHLEPVLACTTGLLVTLLMIWLTRQHEERDRQLSFQQLSLLESSPVLDVAH
                     DLRDLELSGFSALFECSEEVTADEFGRYAEHLALLSEVTWWGWVCQVPEQDRAAFEAC
                     AAQLTGQTDYHIWSPPPAETETVLRRQAQFFPLVHQWPPKAHPELLGCDLGSDLLRNK
                     EFRRVMRNTLTAIVTPRPELVATNRTGILLARATGAQAGSCQHSGLLLANLDVGRWLQ
                     NSLNATPAHRRMTVLDLFELTTDAAPRWLGSTAKQSGLDPVKDWRELPPLTTIRPLLA
                     FGRAYAIVARPTPFFFRLHDSHVGWVALFGAVVTASVAWGLGMLSYRRAILVRLVEEQ
                     TTKLNESAFHYARLA"
     assembly_gap    8847..8946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8947..11252
                     /gene="clpB"
                     /locus_tag="PKI64_01515"
     CDS             <8947..11252
                     /gene="clpB"
                     /locus_tag="PKI64_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOD99717.1"
                     /translation="LLGALGQKNGEAAKLAAGMGITVDSVLQALQAVRGNRRVTDESP
                     EDKYETLKKYGRDLTDEARRGTLDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGK
                     TAIAEGLARRIVAGDVPEGLKNKRVVALDLGAMLAGAKYRGEFEDRFKAFIKEVVDSQ
                     GAIVLFIDELHTLVGAGKAEGAVDAANMIKPALARGELRCVGATTLDEYRKHIEKDPA
                     LARRFQPVLIEEPDVESTIAILRGLKERYEAHHGVRIQDSALVEAAKLSHRYISDRFL
                     PDKAIDLVDEAASRLKMEIDSMPVEVDELERRIMQLEIEREALKKEKDAAARDRLAAL
                     EKNLAELRTEGAALKAHWQREKQLIDAIRQATAELDELHTAEEQATRRSEFEAAAEIK
                     YSRLPAAQKKIQQAQAELAKLQQNRRMLQEEVTPENIAEVVAAWTRIPVSKLLEGERE
                     KLLHMEERLRARVVGQDEAVEAVSRAVRRSRAGLQDPHRPIGSFIFLGPTGVGKTELA
                     RALAEFLFDDEQAMVRVDMSEYMEKHSVSRLIGAPPGYVGYEEGGALTEHVRRKPYSV
                     VLFDEIEKAHPDVFNVMLQILDDGRLTDGQGRTVNFRNTVLIMTSNIGSSAIQETIEK
                     NPRLKRGQPAWNELEKQVLAALRLSFRPEFLNRVDEIILFRSLGIEELRQIVDIQLKR
                     VQGFLAEKNIRLEITEAAEEKLAGEGYDPAFGARPLKRVIQRQVLDALAEKFLAGEIP
                     DGSTVTADYDPDQPQQISFTVQDAPAE"
     gene            complement(11265..11999)
                     /locus_tag="PKI64_01520"
     CDS             complement(11265..11999)
                     /locus_tag="PKI64_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD99718.1"
                     /translation="MIPHAIILLGGKGTRIAAHFPDRPKCLVPVAGRPFLEWQFDWLR
                     RNGICRVLLAAGHLADVLADYLAARPADGLEIALSREPEPLGTGGALKFVEPQLTSDP
                     VLVFNGDSLAPNLEFSTLWKIGGGFFHTVEKKFPHCGKLFMTPSAEAGRFGTVEFDPD
                     GYVTAFREKSAAQAGHVNAGIYLLPRALVAAIPAGRAVSIETEIFPALAAQRRLVAIP
                     IPPPLLDMGTPDGLAAMTAFLARRPA"
     gene            12132..13541
                     /locus_tag="PKI64_01525"
     CDS             12132..13541
                     /locus_tag="PKI64_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOD99719.1"
                     /translation="MAVFLAGWAQWAGADTFTGANWVVRFNLPDQTTSAGSIGPDEFV
                     LRDALLARLDALGNNDWACLATYTFSGNTAAGGAAGPILAAMSNALARGAKLGFVADT
                     GVDVTSNFWPGVSLLSLSKRPGNALQLSRAPPVGGIMHNKIGLFWYRATGQAWVMAGS
                     WNFTGGASSQQWNIMTELQNNTLAAAYSNELRQMLSGYFHSNTNKSRAPDGTRFRLTG
                     TTRDGWVRFAPFSDRTSGGNNALTDIIAAIDDAQDEIFFALNKLTRTEVAAALIRACD
                     RGVVVHGTIPISDRGAPGRDSYEVYQMLLNGANYATLNRVRMYDAYYQAARLRYDNNN
                     RDLTHAKYMVIDPRGVYPLVIQGSANWTASALVLPTSNDENVQFLPHGGIAEAFRAQF
                     AAMTDGMKPWCAWRPGPKASQMTVWLPENEAYQLWFSPDVGLGLESWTPYRPLPATRG
                     IHLLGLTNAAPRGFFRVAP"
     gene            complement(13555..14544)
                     /locus_tag="PKI64_01530"
     CDS             complement(13555..14544)
                     /locus_tag="PKI64_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD99720.1"
                     /translation="MAEKILSLRAATKRKPSARAGRPRPPVSDFAGAQRWATVLAMSL
                     RILHFSDVHLPVPPGAYRLTEIFHPKRGVALINFALRRAAKYRDAQQKLNGLRDFLTR
                     EPVDYLLYTGDTLNFGLEGEFMAGAPRLMETLALARRGALAVPGNHDLYTHGGRALHR
                     KYFAPLLRSDLPEACTPTGFPLVRWLGDDAVAIAFNSARPRLPFWDSSGRVPALELDA
                     LEQLLDMPEIAARRHIFLMTHYPFDEAGYFHGLRNSRALLRVLQGRENLVLLHGHNHR
                     PYTHFLPDSATPVYCAGSLSKAGGESFWFFDLFGTTVNARRGHWRNARFVLAE"
     gene            14543..>15144
                     /locus_tag="PKI64_01535"
     CDS             14543..>15144
                     /locus_tag="PKI64_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOD99721.1"
                     /translation="MKEIIPTLQAWTAAGFASAEAILVSAQHSSPRQPGARLVVNERG
                     ELAGAVSMGCVESDLREHLLRLLRGESPEQIVAYGAAFEPALEVGLSCGGEIKVWLRK
                     HHPDAAAWRGVVALPATARALLLTPLVPDTPQILLHPGDTPPVPAMQPILADLLRRGA
                     NGKFTLEGTTWFAEAIAPEPLLLIVGASPIAVELCAMAAR"
     assembly_gap    15145..15244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15388..16080
                     /locus_tag="PKI64_01540"
     CDS             15388..16080
                     /locus_tag="PKI64_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99722.1"
                     /translation="MIPWEQLTGRRPFNCPQCGGELAADDVNVAQDAALCRACGYQGA
                     FLNALLVPGLSDHDLTHPPKRVQLTRGFGDTLVITCFPARGTLAFLIPFTLFWSGISI
                     WGLIVRPLLAPEAMEWRQILFALPFLLGTLLLLANIAFLIAGRTVLTVSKNQLELYMG
                     AFNIGRRKILARSPEMTVTLAKSNYQVNKVRQPEIVVTSQGRTLRFGAMGIPNDVLPY
                     VAAVLRRTLAGG"
     gene            16091..16801
                     /locus_tag="PKI64_01545"
     CDS             16091..16801
                     /locus_tag="PKI64_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOD99723.1"
                     /translation="MSSIADNLENIRARVAAACAAAGRSATDVRLLAVSKTFGPEAIR
                     EAAAAGQRLFGENRVQEAAAKIPECPGRLEWHLVGHLQSNKAFVAANLFDWIHSVDSI
                     KLLDTLDRHAETVGRRLNVLLQVNVSGERAKSGLPPAEVPAVLARIPHLRNVTVHGFM
                     TIPPLTADPEKARPHFRQLRELRDQWTRDLGLDLPELSMGMTHDMDIAIAEGATIVRV
                     GTGIFGDRPRPAAPAEES"
     gene            16798..17607
                     /gene="proC"
                     /locus_tag="PKI64_01550"
     CDS             16798..17607
                     /gene="proC"
                     /locus_tag="PKI64_01550"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOD99724.1"
                     /translation="MRTEQIAFIGGGNMAEAILGGLLASGVSTPQRLRVADIAPERRA
                     WLAETYAVTVTDDAAEAVRDAAVVILAVKPQQAAEVLSAVAPALSPAQLMVSICAGLS
                     TTTLESWVPARVARVMPNLPALVGKGVSAICGGSRATAADLDLVAELFTAVGLVVRLP
                     ESQMNEVTALSGSGPGYVFAFIEALATTGVQMGLPEDAAWAMARETVRGAAELAAHTG
                     EHPADLRRKVCSPGGTTLAGLAAMEAAGFSAAVAAGMQAARQRAAELAGAS"
     gene            17604..18467
                     /locus_tag="PKI64_01555"
     CDS             17604..18467
                     /locus_tag="PKI64_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99725.1"
                     /translation="MTDNPPAAGRPRSLARPLVRFVVWLTLTMALIYGALQLLVRTDF
                     FRSLVEKELSDLAGMEIRVGRIRATESLNLRLRDTIGVSDVAGIEARLARIRWRLFRP
                     RGEPMLESLRVDGLSLTIAPDESGRLQPQFLGELSRTIFQWTGLPPPAALAELAAPAA
                     TTATPVRATGLASPEQWLRGPLALRGVTVRWQDAKGKVLATASGLDLIWTALVTPNGR
                     RVSHLECNAGDLQLTRGPHIRDLHVELVGVNGKRFLVELTAADWGGATPPPPPGAEAR
                     ALLDSLDSPAP"
     gene            18591..18667
                     /locus_tag="PKI64_01560"
     tRNA            18591..18667
                     /locus_tag="PKI64_01560"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18625..18627,aa:His,seq:gtg)
     gene            complement(18721..19566)
                     /locus_tag="PKI64_01565"
     CDS             complement(18721..19566)
                     /locus_tag="PKI64_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOD99726.1"
                     /translation="MALLAIQMRLDAENFSPGGLDGRWGTKSENALAAWQKKHGHPIT
                     GRVDDTALAALGQTNGLFTTYVITAADHAALTPYPSSWLERSRQERMGYSSIEEMVAE
                     KFHLYRATLRQLNPQAAWPNPPAGSAVTVPAVTAKPLPRLAKIEIRIAQKTLRAYGTN
                     GLLVAHFPCSIAADKAKRPINETLRVVVWAENPEYTFDPALFAEDPAAAAIGKKLRIP
                     PGPNNPVGLAWIGLDRPGYGIHGSPAPEDISKTESHGCFRLTNWDALKLVHAVRDHLP
                     VIVLP"
     gene            19954..21510
                     /locus_tag="PKI64_01570"
     CDS             19954..21510
                     /locus_tag="PKI64_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD99727.1"
                     /translation="MAEHFSVPPVRPTTSAASPPTARLPKPNWGTLLVPLLLLAALLI
                     LPLWVWTFCRIEPGPGEIAILIRKTGTDLPTGQILAMEPGQKGIQLDVLAEGRYFRNP
                     YHWGWQIAKITDIPPGKLGVRTRLFGRDLADGQILAPEGYKGIMAEVLGPGKHRLNPY
                     AYNVQLFDAITIRPGFGGVVTALTGQDVLANDLPEKERNTFLVSAGLKGVRPDILDAG
                     TYYLNPYMVNVVEVNLQSQRFELSGEDAISFLTVDGFTVNVEGTIEYALVRDHVAQLT
                     HQVGDMDDILKKIVLPKARGFSRMEGSKNPAKNYIAGATRQKFQDSLEAHLKTQCLPW
                     GVDIKSVLIRNITPPDAIARIIREREVAVQDANKYGEQIEQAKSEAELVKQEMLAQQN
                     KEKVEADTRRIRAVIAAEQEREVRLTRANRDLEVAKLENDAARAQADARLVRAQAEAE
                     VIRLRNEAEAGVLAGQVKAYGTGINLARNLFYTKIGPQIRAILSGDQGEGLGGLLRGY
                     VPAAAKGGAQ"
     gene            21507..23258
                     /locus_tag="PKI64_01575"
     CDS             21507..23258
                     /locus_tag="PKI64_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD99728.1"
                     /translation="MKKSVSAIVVLVALVAGLWLLWEWGFCRFYVGPDEMAIITAKTG
                     ENLPPGQILAKPGQRGILEDVLGEGRHFRNPWLFDHEIRPVTFIPPGRVGIVTAKVGE
                     DLPPGEFLAEKGQKGIWRNVLGPGKYRLNPVGYQIDVVDAISIPIGYDGVVTSLSGVQ
                     TQPNEFAQRGQKGVRRDVLQPGLYFVNPREYKVDVLEIGVNQVSLMGKGGGAVITKAV
                     QTSQNVAMDRLQSNLLQEQQAKRENYIRSEASSGMRLRAAAPSPAKSGLSSLGGRAVE
                     RQAAMDDKVMPAAPLEMEMPSLSFVLEQFVEFPSRDGFEISLDMTVEFELQPKNVAWI
                     FRSYGDLPAAVDKVIMPQILSVSRLKGSAYGAKDFIMGEGREKFQSELTEALAKTLAE
                     KQIMIHAALIRHVNVPGQILDPIQQASLAQEQNLTNKEKQNTARKLAELNTELSLIDQ
                     RREQVAQETEKIKALIRADEEKQVAEIQGDTMRQTAEIEKQTAGVRAERLTTLGAAEA
                     QVVTLVDGEKAKGEQLKVKAFGDAEAYNLWVLAAALSDKLQINILHTGPGTLWTDLEK
                     AGLAEIGAGSAVQQKQK"
     gene            23267..23929
                     /locus_tag="PKI64_01580"
     CDS             23267..23929
                     /locus_tag="PKI64_01580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOD99729.1"
                     /translation="MADARLTRLLQEITAEAQLTAAYTGRAAFRPATMAALADTPRHE
                     FVRAAEQLAAYLDMPLPIGHGQTISQPYIVALMTDLLDLPPAAKVLEVGAGSGYQAAV
                     LSRLARQVHTIEIVPALAIACRERLARLGYANVVVHEGDGGLGLLAEAPFEGIMVTAA
                     APRIPPALVDQLAAGGRLVIPVGAVDAVQELVVVTKDARGATNSRTVLSVRFVPLTGQ
                     AG"
     gene            complement(23926..24606)
                     /locus_tag="PKI64_01585"
     CDS             complement(23926..24606)
                     /locus_tag="PKI64_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99730.1"
                     /translation="MKRMVLVSWMLLSLAVGAMAQTEEPTMAAPVCRPLTCFSLGVHF
                     SFWDAADLHDFDIAGAVGGGVIGQVRLHKHLALDLRLSGYASGFSRDVYEEGLGWSDD
                     DFTLVAMPFELGVVGILPMGQKVSVYGGPGVGYYLFDGQFTRKRGKATTIYDVEMADN
                     PGGYLLGGLKMQLTGNLALYCEGKYVWVDTTVRTPARSRDEWGLHDIRVNLDMSGLMV
                     NAGLLFTF"
     gene            24814..25767
                     /gene="sppA"
                     /locus_tag="PKI64_01590"
     CDS             24814..25767
                     /gene="sppA"
                     /locus_tag="PKI64_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOD99731.1"
                     /translation="MLGFVLGLSLLLNLAAVGILVEGAESTGGEGEDEYPQFTETHSY
                     GAGTTKVVRIGFSGVLTRELDGGWLGSSDPIEECQRQIRAARQDKKVAAIIFEVNSPG
                     GEVTAADDIYRELTLFKESREDRKLVVLVRDYAASGGYYISLPADHIVAQPTATIGSI
                     GVLLQTYNVRGLSEKLGVTDTTIKSGRNKDMLNPFQPVDPEQVALLQGNVDAMYERFL
                     GLVATARGVTKNKLRPLADGRIFTADEALKHKLIDSIGYWDEACATTADLLGVEELRV
                     VAYESRQSLFNMLFSARMPLPNWRAWLHRAATPRIQYLWQY"
     gene            complement(25805..26983)
                     /locus_tag="PKI64_01595"
     CDS             complement(25805..26983)
                     /locus_tag="PKI64_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD99732.1"
                     /translation="MKTRSRVAEHVRQLPFSGIRKFFDIVAQRKDVISLGVGEPDFDT
                     PWTVREAAVFSLERGATHYTSNRGDPALRKAICRYVARQFGAEYDWESEVLVTVGVSE
                     AIDVALRTIINPGDEVLYHEPCFVSYRPSIILAGGVPICVETRREDEFRLTRAMLEKA
                     VTPKTRVLMLNFPCNPTGAVLAKQDVEDIAAFCVQHDLLLLTDEIYAELTYDSKHVSF
                     VSIPAMKERTIFLHGMSKAWAMTGFRIGYACAPAEISENMMKLHQYAIMSAPTTGQKA
                     AAEALERGDSAVADMVAEYNRRRNYLIASFAEMGVPCHTPAGAFYAFPYIGDLGISAE
                     DFALRFLEEKGVAVVPGDAFGQCGAGFLRCAYATSMDNIREAMKRMSEFVAAVRTEKR
                     "
     gene            complement(27005..27484)
                     /locus_tag="PKI64_01600"
     CDS             complement(27005..27484)
                     /locus_tag="PKI64_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOD99733.1"
                     /translation="MDELLTILQENARTSLEDIARMLRSTPEAVAARIAEYEKNGTIR
                     GYRALVNEDLLQQDNVTAVIEVRVQPEREGGFDRIARRISGFPEVVNMYLMSGKYDLL
                     LFVEGKNLREVASFVSERLSTLDGVLSTGTHFMLKTYKQDGVLMDGDKVDERLQISP"
     gene            complement(27590..29665)
                     /locus_tag="PKI64_01605"
     CDS             complement(27590..29665)
                     /locus_tag="PKI64_01605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF007209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOD99734.1"
                     /translation="MTDTRQSSLFDDDEEPAPRPARGKSTPPSIPAASKQPVSDSDIG
                     DGPLRRLMDGNFLQYASYVIRDRAIPELADGLKPVQRRILHALHEKDDGRFIKVANIV
                     GHTMQYHPHGDASIGDALVTLVNRGYLIEGQGNFGNIHTGDPAAAPRYIECRLTDLAA
                     TQLFNDDLTEFIPSYDGRNKEPVVLPAKLPLLLMLGAEGIAVGLSTRILPHNFGELIQ
                     AQIAILQKKPFKLLPDFQQGGLVDAREYDEGRGKVRCRAVIDQKDEATLVIRELPFGV
                     TTEALIGSIEDAAKKGKIKTKAIRDYTAEKVEIEIALAADTDIQQAIQSLYVFTQCEV
                     SLSLRPIVIHEQRPVELSVHEILRHNTRRLVELLRKELQIERKKIDEALHAASLVRLF
                     IVNRIYKRIEECKTAPDVRQAILNGLAPFRDQLRRDITNADLDMLLAIPIRRISLYDI
                     NKSRKEEDDLLADLASVEEHLTDVTRFTITYLKGIYRDYADLFPRKTREARFDAIEVR
                     ELTAEEHAVVFDREKGYLGYDVGGGEPLFRCSSYDKIILVWKDCRYRVIPPPAKLFVD
                     QDLVYAAIYDRDRVMTLVYEADGISHLKRFTFGGVIQNKEYVCAPEGEPAICHFFSDA
                     QPATLYVKYAPRKGQQIHQQEFDPKKVAIRTPKTRGIQMTTKKIAAISAQKPRNWDDK
                     AGPRGALLD"
     gene            29870..31000
                     /locus_tag="PKI64_01610"
     CDS             29870..31000
                     /locus_tag="PKI64_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOD99735.1"
                     /translation="MSKQKAQAPEVISKAAAARKIVAVAQEIHDDIVKKRKKPAMSFP
                     VRSLANVKYDAKRGHFEILGKRATRTLSYNTVKAFAQSMRLLATTKNDLLDRDDIAGK
                     REVYYNSKGWGECRFDEQPESDALLDDMEAMLGVNREQLGYIPEERGGDVCGELTVID
                     QDPQTGADVKINCAKLGSGAWSIPSRVEHLRFETRAKLVLVVETASLFQRLVHHRYFE
                     RANCVLISMSGVPTRACRRFIRRLADDRNLPVLAFTDGDPYGYCNIYRTLKVGSGQAA
                     HINRFFCVPQAHYLGVTPQDILDYQLEDATHPLEEVDIKRAKDALKNDPFIRHHKEWQ
                     QVLEQMLQMGVRIEQQAFAKHGLNFVLDTYLPEKIKKGKFLP"
     gene            complement(31467..32528)
                     /locus_tag="PKI64_01615"
     CDS             complement(31467..32528)
                     /locus_tag="PKI64_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOD99736.1"
                     /translation="MSPPTPAPGASLAKAALPASGLPARILAVLRAHGFITLADLRKP
                     LPTDHKLDADDRALLARIAAYAAATCAQRPPALNLREWLALFLPARQADAIQLHFAFD
                     DPTASLTRHEMRLRETGFKLGVTRERVRQLLNLALGTLRQALPLQAAEPFYRAATVTL
                     QAGGGVLEPAEVAAQAAPIWGDASPVGAFLLLVQLVPGRLIRYRDFFSLYAATAIERA
                     EKALVDRLVAAPGLQSLATLAARLPKAARPAGASDFAPLLRALLRHRTDTLLTLDDRA
                     GLAARDTPELLREVLAANGEMSLRALVAAFNAGLPPACQRGSGFLRAVLLNDPLIRKT
                     APNRYALPGGLQANLPLDS"
     gene            complement(32525..33913)
                     /locus_tag="PKI64_01620"
     CDS             complement(32525..33913)
                     /locus_tag="PKI64_01620"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOD99737.1"
                     /translation="MTTPTRLEHDSLGPRPVPATALYGAQTSRSLENFPLSGAPLPRE
                     IIHGMALLKLAAAKANAALGLLPETKAQAIIAACRAILTGAHDAEFPVDLFQAGSGTS
                     SHMNLNEVIANLANEALGGQRGAKAPVHPNDDVNMGQSTNNSFPSGAKIAVLNQMPAL
                     IDALNALAQALQARATEFAPILKAGRTHLQDAVPITLGQEFAAYASAIRAATARIRQA
                     ADELLELGAGGNAIGTGINTKPAFRAEIIRALQGESPHPFRVAADGIHATQFMSSFAH
                     VSAALRNAAFDLNQITNNLRLLASGPNTGLGEIRLPPVEPGSSIMPGKINPSICEAVN
                     MVCLHVQGLDHAVALACGAGQLELNTHMPLIGAHTLQAAQLLTNAARTLAEKCVAGIT
                     ADAAACRRNLERSAALATLLNPRLGYDRVTKLVQESLATGQTLKELALAQKLLTAEEY
                     EALLQAATAPNL"
     gene            33994..>34223
                     /locus_tag="PKI64_01625"
     CDS             33994..>34223
                     /locus_tag="PKI64_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99738.1"
                     /translation="MWKKRHMVYYVSAHGYGHGVRASDLIGGLLDVAPELRLTVISDL
                     PEDFFRSRLPAADGRVAFAPGAFDVGMVQRDS"
     assembly_gap    34224..34323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34324..34767
                     /locus_tag="PKI64_01630"
     CDS             <34324..34767
                     /locus_tag="PKI64_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99739.1"
                     /translation="LSFTTLGWDADALRAVGGLAEYEFFTVKPLAWRGQPNLHAVDRH
                     AFSFSDVLASVDFVITKPGYGILSDCVANRKPIIYAEREDFIEYPLLVQELRRYLAHV
                     HIPAADLYAGHLAAALRAAETAPPPPEQLAFGGARVAAELLLNVG"
     gene            complement(34778..35740)
                     /locus_tag="PKI64_01635"
     CDS             complement(34778..35740)
                     /locus_tag="PKI64_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOD99740.1"
                     /translation="MKKTASLLLWLAAGGLAVVSPLSAQTTVRVTGDPVNLRAKNLLT
                     SEVVTQAHYDDRLTAYEIGEEWVCVAAPTSAEAWIMKTYVQTPANTIGTKRVNVRAGP
                     SINYNILCTLELGATVKPTGNVQGDWLQIEPPAEARVWIHRDYVEVLTTDSATVAAAE
                     PEPVPEAKKSARRKKPATDADSAAPAVVAKPAAETPAVLPTPVIAASVPEKDDAVRTI
                     PVPAPADLKLVPLSGQGAMVEVRGELRAAPLINEAPTRYRVVQWQDNRWVILCHVYGQ
                     ATKFRSLQNKQVRLEGRQYWIQGAAAPVLVPTQIQEIASESVAP"
     gene            35834..36808
                     /locus_tag="PKI64_01640"
     CDS             35834..36808
                     /locus_tag="PKI64_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD99741.1"
                     /translation="MATQPKHRGLGRGLDALIKGSGPRAAETGKPPARPAAPPATAAV
                     PPAKDQVRRVAVQKIKPGAWQPRRVFAADALQELADSIREKGVLEPVWLRQVGDMFEL
                     IAGERRFRAAQLAGLAEIPALLHEVSDVEAREMALIENLQRADLDLIEEAEGYRDLMQ
                     AAALTQEEVARRVGKARATVANALRLLDLSSAIKSALSAGTLSAGHAKVLLGVAAPDR
                     DLLATRVIAQGLSVRALEKLVAGLDRTAPAKKKTDPAKTKPASDQSARQLKFLADRMQ
                     QELGTKVTLTPARVLPDGKRAMGQIVIEYFDNEDLSRLLDMLNLGDLA"
BASE COUNT         6772 a        10878 c        12123 g         6674 t
ORIGIN      
        1 gagttgacgg ccagtttctg ctgctccaag ccatcgcgca tttcccgcat gccgtgggcg
       61 atgcagtgcg aataggcaat gatgatggag gggccgtcat gggcttcggc ttccttgaag
      121 gctttgatag cttgcggcgg gttggcgcca aaggccacac gggcgacgta cacgttctcg
      181 taggacatcg caatcatggc gaggtccttc ttggccaccg gtttgccgcc agcggcaaac
      241 ttggcaaccg cgccccgcgg cgtggacttg gaggcctgtc cgccggtgtt ggaatatacc
      301 tcggtgtcga gcaccaggat gttcacgttg gcgccggagg cgagcacgtg gtccaaaccg
      361 ccatagccaa tgtcgtaagc ccaaccatcg ccgccgacaa tccagacgga acgctggatg
      421 aggaagtcgg ccaccgcaag cagattaaag gcctcgggct tggtgttgcc ggcgagtttc
      481 ttcttcaact ctgccacgcg ggcgcgctgc gccgcgatgc cggcttcgtc cttctgatca
      541 gcttgcagga tgccatcggc caattctgcg ccgaggtcat ccttgagggt ggccaccaac
      601 tgttcggcgt agagcttttg ctggtcgctg gtgaggcgga agcccaagcc gaattcggcg
      661 ttgtcctcga agaggctgtt ggaccagacc ggtccacggc catcgcggtt ctgggcccac
      721 ggggtggtgg gtagattgcc gccgtagatc gaggagcagc cggtggcgtt ggccacgacc
      781 gcgcgatcgc cgaagagctt ggagagcagg ctcaggtaag gcgtctcgcc gcagccggcg
      841 caggcgccgg agaactcaaa cagcgggagc gtgtattggg catccttgac catgttcaag
      901 ttgagcttgg cgcggtcaat cagcggcagg gtttcaaaga atttgatgtt ctcccgttcc
      961 tgggccacga ttggcaattt ggggccgaga ttgatggact tgcgcgtcgg gtcgttcttg
     1021 tttttcttgg ggcaggaggc cacgcaaacg ccgcaaccag tgcagtcttc gacgtagatt
     1081 tgcagggtgt aggctttgtc cggcttgttg cgcacggggg cgtgcttgaa gccggcgggg
     1141 gccttgtcga gcaaggattt gtcgtattcg ttggcgcgga tgcaggcgtg ggggcacaca
     1201 aaggagcagg tgccgcactg gatgcactcg ttctcgtccc agatcggcac ggagagcgag
     1261 acgttgcgtt tttcccaccg ggtggtggcg gaggggaagg tgccgtcaat cggcatcttg
     1321 gaaaccggca gcgaatcgcc atcgccgacc atgatggtgg cgatgacttc cttgacgaac
     1381 tccggcgcct gcggggagac cgtcggcggg atttcgatgg tggaggtcac ggcgctgtac
     1441 gggatttcgt acaggttggc gagggtttca tccacggcct tgaagttctg ctggacgata
     1501 tcatcgccct tgcggccgta ggtcttctgg atggagtcct tgatcttggc aatggcttct
     1561 tccttgggca gcacgccgga aatagcaaag aagcacgtct gcatgatggt attgacgcgc
     1621 gtgcccatgc cggtgcgttc ggccaccgtg taggcatcaa tgttgtagaa cttgattttc
     1681 ttttcgataa tgcgcgcctg caccttcttg ggcagcttgt cccacacctc ttccttgctg
     1741 aacgggctgt tgagcaggaa ggtggaaccg gtggaggcgt agcgcaggat gtcggtctgc
     1801 aacaggaatt cgtactggtg gcaggcgaca aagctggcct tttggatcag gtaggggcta
     1861 tgaatcgggt tggggccgaa ccgcaggtgc gagatcgtgc gcgagccgga cttcttcgag
     1921 tcatacacga agtagccctg cgcgtagaaa tcggtttcct cgccaatgat cttgatggag
     1981 tttttgttgg cgcccacggt gccgtccgcg ccgaggccga agaacatggc gttggtgacc
     2041 tcggggtggc ggatgaagaa tgtggtgtca atgtcgaggt gggtatgggt gacatcatcc
     2101 acaatgccga cagtgaagcc attcttgggc gcgtccttct tgagctcatc aaagaccgcc
     2161 ttgaccatgg cgggggtgaa ctccttggag gagaggccgt agcggccgcc gatcaccttg
     2221 ggcatggtcg ggaacgagcc attcgccacg ccttgggcga gagcggagag cacatccttg
     2281 tagagcggct cgccatcgct gccgggctcc ttcgtgcggt cgagcacacc gatggccttg
     2341 acggacttgg gcagcgccgc aaagaaatat ttggcggcaa acggccggta caagcgcacg
     2401 acgatggcgc cgactttttc gccctgcttt tgcagcactt cgatggtttc cagcgcggtt
     2461 tccgcgcccg agcccatcag cacgatcacc cgctcggcat ccggcgcgcc gatatagtca
     2521 aagggccggt actggcggcc ggtcagcttg gcaaactcat ccatcgtggc ggccacgatg
     2581 tccgggcagg cctcgtagaa cttgttggcg gcttcgcggc cttggaaata cacatccgga
     2641 ttctgcgccg tgccgcggat gaacggcttt tcgggattga gcgcccgcgc acgatggtcg
     2701 gccacgagtt ggtcgctgac catggcgcgg atgacatcgt cagaaatcat ttcgattttg
     2761 ctgatttcgt gcgaggtgcg gaagccatca aagaaatgca ggaacggcac ccgagccttc
     2821 agcgtggcgg cttgggcaat cagggcggtg tcgagcactt cctgcacgct attggaggcc
     2881 agcatggccc agccgatctg ccggcaggcc atcacgtcct gatggtcgcc aaaaatggac
     2941 agcgccgagg ccgccagcga gcgcgccgaa acgtggaaca cggtcgaggt caactcaccc
     3001 gcgatcttat acatattggg gatcatcagc aggaggccct gcgaggcggt gaaggtggtc
     3061 gtcagcgcac cggtttgcag cgcaccgtga accgcgcccg ccgcgccgcc ttcggactgc
     3121 atttccacca cggtgggcac cgcgccccag atgttggttt tgccttcgct catccattca
     3181 tcggcccatt cgcccatgtt ggaggaaggc gtaatcggat aaatggcggc aatttccgtg
     3241 gtcttataag cgatgtgggc ggcggcttcg ttgccgtcaa tgatggcgat ctgtttcttt
     3301 ttgctcatgc ggtgctccgg ttggtttcta aatcgtacct gtgttgttgg caaatagacg
     3361 ccaaaacaca cggggcggac tgtagcaaag cagcgggttg gcgcaatcat aaaatgcggc
     3421 gcaggcgcag aaaaatcgtt ttacatcgcc caactcgcca ttggcgaggc cgtcgcgggg
     3481 cttgaaaacg gctccgcgag gcttaacttg gcttttggcg cgcggcagaa aaccggcaaa
     3541 aagggccctc cgggggcaaa atggcgaatc cgggggtgtt cgctgccgat tttgcggcac
     3601 aatgggcggc agtacttgcg cataccgtgg tgttttgtgc cgtgaatcgg cgcacaaacg
     3661 acgctcgcgt catctttggg tagggtcagc aaaaaagtag tgcggtattt ttttcacaca
     3721 aaccgcggcg ggaggcgtat agttcccgct tcaaaacaga acccgggttt ggttatgagt
     3781 tcatttatca atataatgcc gtgtttggac atccgcgccg ggcgcgtggt caagggtatt
     3841 caatttgatg acctgattga tgccggcgat cctgtcgcca aggctgtggg ctatgaagcc
     3901 gatggcgccg acgagctggg gttcctcgat atttccgtgg caatggacgg caccaagccg
     3961 aactttgcgc tactgcgcga ggtgacatct gcggtgaaga ttccggtgac catgggtggc
     4021 ggcattcgct cgcgcgagga tgtggctgct gcgctggcgg cgggggcggc caaggtgagc
     4081 ctggccacgg ccgcatttcg gcagccagat ttggtgggtc acctggtggc ggaattcggg
     4141 agcaacacca tcgtggtggc actggacgcg caatttaatc ccaagatgcc gtccgagcgc
     4201 gaagtctatg tcggcggcgg caagattccg actggcgcgg atgcggtgga gtttgcccgc
     4261 cgcatggccg atgccggcgc gggttccttc ctgcccacca gcattccggc cgacggctcc
     4321 aagcagggct acgacgtgga attgatccgc gccattgccg atgccacgcg ccggcccgtg
     4381 attgccagcg gtggcgcagg ccttttggtg cattttcgtg aagcggtccg gcagggccac
     4441 gccagcacct tgctgctggc cggcgcgctg cacttcggcg tgttcaacat tccgcagatc
     4501 aagacgtatc tcgccgcgga gcgcattgcg gtgcgcaacc cgccgacgcc cggcgtctaa
     4561 cgatcctgca gcaggacggc ccgtactttc tgggccagtt gcagccgcgt gaagggcttg
     4621 gccaggaagt gcgcgggatc aatggcatca gggccgtcga gattggcggg gtaaccggac
     4681 atgaacaggt gacgcgcgca cgggaagcgc ttttttatct cctgactcaa ggcgattccg
     4741 ttgctcggtg gcaggttgat gtcggtcagc aataggtcaa tgttgcaatc cttgccgcgg
     4801 cacagtttgg cggcttcgtt cgcggaactg gcagtcagca ccacgtagcc caatgattcc
     4861 agcatgctgc gggtgacgcg caggatggcg ggctcatctt ccaccacgag gatgatttcg
     4921 tggttgggcg cttgcaattc gacccactcg gtggcggtgg cttccgttgg gggaatgccg
     4981 ctggcaaggc gcggcagcag gatgcggaac tcgctgccct ggcccaccac gctgtgcact
     5041 tggatacagc cgttgtggcg cttgacgatg ccatacacgg tggagaggcc gagaccgctg
     5101 ccttggccca cgggcttggt ggtgtagaac ggctcaaaga cgtgctccac cacatcgggt
     5161 ggcatacccg cgccgttatc gcgcacggtc agcagcacgt atgatcccgg cggcaggtcg
     5221 cacggcgtgg ctggcccatc gcggacaatt tccatgacgg cggtggccag cgtgatgcag
     5281 ccattggggc cgacggcatc gcgcgcgttc aggcacaggt tgacgatgat ctggtccaac
     5341 tggctggggt ccacgtgaat ccagcccaca ttctcgccgg ggtgccactg caattggatg
     5401 ccttcgccca ccaaacgccg cagcatatcc agcatgttgt ccagcgtggg gttgacttcc
     5461 agcgtctgca aggccgcggg ctgcttgcgg gcgaacgtct gcaattggcg gatcagggtg
     5521 gtggagcggg tggctacctt ttggatttcg cgcaggtcgg aatggatggg ctggccggcc
     5581 gcggtctgtt ccaaggccaa ctcagtatag cccagaatgg cttgcagcat attgttgaaa
     5641 tcgtgcgcca cgccgccggc cagccggccg agggtttcga gtttttgcgc atggtcaagt
     5701 tgggcctgca gagcggcctt ttcgttttcg gcgcgaattt gcgcggtgat gtcgcgcgcg
     5761 gcggccacat aattaacgat gcgtccggtg gtgtcgcgca cgggggagat ggtggcctgc
     5821 tccgtgtaaa tcgagccgct cttgtggcgg ttgacgaagc gccctgtcca cgtttggccg
     5881 gcggccaaca cgctccacag gttttggaaa aactccggcg gctggacatc actggccaga
     5941 atggagggcg tgtgacccaa cacttcctcg cgggtgtagc cggtggtgcg ggtgaaggcc
     6001 ggattgacga agacgatcat cccggcggca tcggtaatga ccacgctttc atcggtttgc
     6061 tcgatggcgc gcatcaggcg ttcgcgctcc tgttcggtct gtttgcgctc ggtgatgtcg
     6121 cgcgagacgc cgtgcaattc caccttgccg gtgctggggt tgtgccagag gcgggagacg
     6181 gtttccaccg tgatgcgcga gccatccttg cagcgcggtg acagctccac cacaaagaaa
     6241 tcattttcgg tcagctcgcc gctgcggaaa cgcgccaacg cttttttcaa caggctgcga
     6301 cgctcatgcg cttcttccgg cggcagggaa tccaccagcg taccggccat gacttcttct
     6361 ggtgtgtagc cgcgcacccg cttgacggtg gggctcacgt agaggaagcg caaggtgtcg
     6421 gcatccgcga tccagaccac gtcgcgcatg ctttcggtca gcatgcgata cttctgctcg
     6481 ctgctgcgca aggcttccac cgcgcggatg ttttcggtga tatcaatatg cgtgccgatg
     6541 agcgaggcag gctgttgcgc cgtgccgccg gtcagccggc cgcgcgacca aatccacgcc
     6601 cacgagccat ccttgcgccg catccggaac cgcaggtcat aaaatccctg cggattcgcg
     6661 atataattgg ccaaataggc tttcgcggcc gggcggtctt ccggatgcac aaagttttcc
     6721 caggcagttt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgccaacc
     6841 gggcatagtg gaaggcgctt tcgttgagct tggtggtttg ctcctccacc agccgaacca
     6901 ggatggcccg ccggtaggaa agcatcccca gcccccacgc cacgctggcg gtcaccaccg
     6961 cgccgaagag cgccacccac cccacatggg aatcatgcag ccggaagaaa aacggggtgg
     7021 ggcgggcgac aatggcgtag gcgcggccga aggccagcag gggccggatg gttgtcaagg
     7081 gcggcaattc gcgccaatct ttgaccggat caagcccgga ctgtttggcc gtggagccca
     7141 gccagcgtgg cgcggcatct gtggtgagtt caaacaggtc cagcaccgtc atccggcgat
     7201 gggcgggggt ggcgttcaag ctgttctgga gccagcggcc cacgtccaga ttggccaaca
     7261 gcaggccgct atgctggcag gaacccgcct gcgcacctgt tgcgcgcgcc agcaaaatgc
     7321 cggttcgatt ggtggcaacc aattccgggc gcggcgtgac gatggcggtc aacgtgttac
     7381 gcatgacgcg ccggaattcc ttgttgcgga gcaaatcgct gcccaaatcg cagcccaaca
     7441 attcggggtg cgccttgggt ggccactggt gcaccaaggg gaaaaattgc gcctgccgcc
     7501 gcagcacggt ttcggtctcc gcggggggcg gggaccagat gtgatagtcc gtttgcccag
     7561 tgagctgcgc cgcgcatgcc tcaaatgcgg cacgatcctg ctccggcacc tgacacaccc
     7621 agccccacca ggtcacttcc gacaacaacg ccagatgttc cgcatagcgg ccgaattcat
     7681 cggcggtgac ttcctcgcta cactcgaaca gcgccgagaa gccgctcaat tccaagtcgc
     7741 gcaagtcgtg ggccacatcc aaaaccgggc tggattccag caacgataat tgttggaacg
     7801 acagttgccg gtcgcgctcc tcatgttggc gcgtcagcca aatcatcaag agtgtgacca
     7861 gcaggccggt cgtgcaggcc agcaccggct ccaaatggcg caagcggcgc tgccgcgcga
     7921 gggggccgtt cgcccgccgt cggagcagtg ccacgcccac cacaccctga agcaccagcg
     7981 ccagccagaa cagggctgga atcacggcgg cgcgggcgca cagccgcagc cagcggtcgg
     8041 caggcacatc aatgccaagc gcggagatca ttttgccggt acgcgggtct ttgatcggca
     8101 ccaaggcgct gacccaagtg ccccagcggt cgcgcgccgg ccccccggta ttggcgaggc
     8161 cttgctcata tagctcgcgc agttgggggg gggcctccgg ataaagctgc ccgggcggtg
     8221 aaacctccgc cgtgtcggcc gctaccgagt ccagttgaaa ataaaattca ccatcggcat
     8281 tgcggcccaa gagatacacc cattgccaca gcgggttgat ctgctgcgcg gccatcaact
     8341 ggcttttgag ccgccggtat tccggtcgcc cgatatcgcc gggctgcccc gtcaactttt
     8401 gcagattact ggcgctgatg gtttgcccca attggtgcgc ctgtcccagc aggtgttcgc
     8461 gcagccattg gtccttggcc cataccgccc agccgatgaa caccacgccg gccgccatcg
     8521 ccgccagcgc gatccaaaat gcaccccagc cgaggcggag aggggcctcg ttcacgggcg
     8581 gcgggcccgg cggcgattgc tggttttccg gagcgcggtc catggtgcag aagaaagaat
     8641 agactgagcc ccgccgcccc gcaatgccaa ttacgcggcc cgccaccgtt tgaattggcc
     8701 cgttttttat ggtactgtac gtagttgaga aaggaaagaa atcccatggc gtatgacaac
     8761 ctgaccacca aggcccaaga ggccctgcgc gatgcccaac ggctggccac cgagcttcag
     8821 cacggcgaag tggataccct ccacctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnntctt gctgggcgcg ctcggccaga agaacggcga agccgctaaa ctggccgccg
     9001 gaatggggat caccgtggat tccgtgctgc aagccctgca agccgtgcgc ggcaatcggc
     9061 gcgtgacgga tgagtcgccg gaagataaat atgaaacatt aaagaaatat ggccgcgacc
     9121 tgaccgacga agcccgacgc ggcacgttgg accccgtcat cgggcgcgat gccgaaatcc
     9181 gccgcgtggt gcaggtgctc tcgcggcgca ccaagaacaa cccggtactg attggcgaac
     9241 cgggagtggg caagacggcc atcgccgaag gcctcgcccg ccgcatcgtg gccggcgatg
     9301 tgcccgaggg cctcaagaac aagcgcgtgg tggctctgga cctcggcgcc atgctcgccg
     9361 gcgcgaaata tcgcggcgag tttgaggacc gcttcaaggc gttcatcaag gaagtggtgg
     9421 atagccaggg ggccattgtg ctgttcattg acgaactcca cacgctggtt ggcgccggca
     9481 aggccgaggg cgcggtggat gccgccaaca tgatcaagcc cgcgctggcc cgcggcgagc
     9541 tgcgctgcgt cggcgcaacc accctcgatg aataccgcaa gcacatcgaa aaagaccccg
     9601 ccctggcccg ccgtttccag cccgtgctga tcgaggagcc cgatgtcgaa agcaccatcg
     9661 ccatcctgcg cggcctgaag gagcgctacg aagcccacca cggcgtgcgc atccaggata
     9721 gcgcgctggt cgaggccgcc aagctctcgc atcgctatat ctctgaccgc tttctgcccg
     9781 acaaggccat tgacctcgtg gacgaagccg ccagccgcct caagatggaa attgacagca
     9841 tgcccgtcga ggtggacgag ctggagcgcc gcataatgca gctggagatc gaacgcgagg
     9901 cgctcaaaaa ggaaaaagat gccgccgccc gcgaccgtct cgccgcgctc gaaaaaaact
     9961 tggccgagct gcgcaccgaa ggcgcggcgc tcaaggccca ctggcagcgc gagaagcaac
    10021 tcattgacgc gattcggcag gcgaccgccg aactggatga actgcacacc gcggaggagc
    10081 aggccacccg ccgcagcgag tttgaggccg ccgccgaaat caaatactcg cgcctccccg
    10141 ccgcccaaaa gaagattcag caggcgcagg ccgagctggc caagctccag caaaaccgcc
    10201 ggatgctgca agaggaagtc acaccggaaa acatcgccga ggtggtcgcc gcctggacgc
    10261 gcatccctgt cagcaagctc ctcgaaggcg agcgcgaaaa attgctgcac atggaagaac
    10321 ggcttcgcgc gcgcgtggtg gggcaggatg aggccgtcga ggccgtctcc cgcgcggtgc
    10381 gtcgttcccg cgccggcttg caggaccccc accggcccat cggttcgttt attttccttg
    10441 gcccgaccgg cgtcggcaaa accgagctgg cccgcgcgct ggccgaattt ttgttcgacg
    10501 acgaacaagc catggttcgc gtggacatga gcgaatacat ggagaagcat tcggtgagcc
    10561 gcctgatcgg cgcgccgccg ggctacgtcg gctacgaaga gggtggcgcg ctgaccgagc
    10621 acgtccgccg caagccgtat tccgtggtgc tgttcgacga aatcgaaaag gcccacccgg
    10681 atgtcttcaa cgtcatgctg caaatcctcg acgatggccg gctgaccgat ggacaagggc
    10741 gcaccgtcaa cttccgcaat acggtgctga tcatgacctc caacatcggc agtagcgcca
    10801 tccaggaaac catcgaaaag aacccgcgcc tcaaacgtgg ccagcccgcg tggaacgagc
    10861 tggaaaaaca ggtgctcgcc gcgctgcggc tgagcttccg gccggagttt ctcaaccgcg
    10921 tggatgaaat catcctgttc cgcagcctcg gcattgagga gctgcgccag attgtggaca
    10981 tccaactcaa gcgcgtgcag ggcttcctcg ccgaaaagaa tatccggctc gaaatcacgg
    11041 aagccgccga ggaaaaactg gccggcgaag ggtacgaccc cgcctttggc gcccgccccc
    11101 tcaagcgcgt gatccagcgg caagtcctcg atgcgttggc cgaaaagttt ttggccggcg
    11161 agattcccga tggcagcacg gtcacggcgg actacgaccc tgaccagccg cagcaaatct
    11221 cgttcacggt gcaggacgcg ccggcggagt aatcacgcga ttttttacgc ggggcgccgc
    11281 gccagaaaag ccgtcatggc ggcgaggccg tcgggtgtgc ccatgtccag cagtggcggc
    11341 ggaatgggaa tggccaccag ccgccgctgc gccgccagcg caggaaaaat ttccgtctcg
    11401 atggagaccg cgcggccggc gggaatcgcc gccaccagtg cccgcggaag caggtaaatg
    11461 ccggcgttga cgtggccggc ctgtgccgcg cttttttcgc ggaatgcggt cacgtagcca
    11521 tccggatcga actccacggt gccgaatcgc ccagcttccg cgctgggggt catgaaaagt
    11581 tttccacagt gtggaaactt tttttccaca gtgtggaaaa atccgccgcc aattttccac
    11641 agtgtggaaa actcgaggtt gggggccagc gaatcgccgt tgaacaccag caccggatcg
    11701 ctggtcagtt ggggttccac aaatttgagc gcgccaccag ttcccagcgg ctccggctcg
    11761 cgcgaaagcg caatctccag cccatcggcg gggcgcgccg cgaggtagtc cgccagcacg
    11821 tcggccagat gcccggcggc cagcagcacg cggcagatgc cgttgcgtcg gagccagtcg
    11881 aattgccatt caaggaaggg gcggccggcc acgggtacga ggcatttggg ccggtcggga
    11941 aagtgcgcgg caatccgggt gcctttgccg cccaataaaa tgatggcgtg cggaatcacg
    12001 ccggcactat gcccaagccg cgctgtggaa gtcaaacccg cgcccgtggc gcggcggcgc
    12061 gggcggagtg aattatggat ttgtccgccg cggcggcagt cggtagtctg cccccgatgc
    12121 gcaggatctt catggcagta ttcttggcgg ggtgggccca gtgggcgggg gccgacacct
    12181 tcaccggcgc caactgggtg gtgcgcttca acctgccgga tcagaccacc agcgccggca
    12241 gcatcggccc cgatgagttt gtgctgcgcg atgccctcct ggcgcggctg gatgccttgg
    12301 gcaacaacga ctgggcctgt ctcgcgacct acacgttttc aggcaatacc gcggcggggg
    12361 gcgcggcggg gccgattctg gcggccatgt ccaacgcgct ggcgcgcggc gcaaagttgg
    12421 gctttgtggc ggatacgggg gtggatgtga catccaactt ctggccgggg gtgtcgctgt
    12481 tgagcttatc caagcgaccg ggcaacgcgc tgcaactttc gcgcgcgccg ccagtgggcg
    12541 gcatcatgca caacaagatc ggcttgtttt ggtaccgcgc aaccgggcag gcgtgggtca
    12601 tggcgggctc gtggaatttc acgggtggcg cgagctcgca gcagtggaac atcatgaccg
    12661 agctgcaaaa caacacgctg gcggcggcct attccaacga gctgcgccag atgctgtcgg
    12721 gctattttca ttccaacacc aacaaatcgc gcgcgccgga tggcacgcgc ttccggctga
    12781 cgggcaccac gcgcgatggc tgggtgcgct tcgcgccgtt ctccgaccgc acctccgggg
    12841 gcaacaacgc gctcaccgat atcattgccg ccattgacga cgcgcaggac gaaatctttt
    12901 ttgcgctgaa caaactcacg cgcacggaag tggcggcggc gctgatccgg gcgtgcgacc
    12961 gtggcgtggt ggtgcacggc accatcccca tcagcgatcg tggcgcgccg gggcgcgaca
    13021 gctacgaggt ctatcagatg ctcctgaacg gcgcgaatta cgccacgctc aaccgcgtgc
    13081 ggatgtatga cgcctattat caggccgcgc gtctgcgcta tgacaacaac aaccgcgatc
    13141 tcacccacgc caaatacatg gtgattgatc cgcgcggcgt gtacccgctg gtgattcaag
    13201 gctccgccaa ttggacggca tcagcgctgg tgctgccgac atccaacgac gaaaacgtgc
    13261 agttcctgcc gcacggcgga attgccgagg cctttcgcgc gcagttcgcg gccatgaccg
    13321 acggcatgaa gccgtggtgc gcgtggcggc cggggcccaa ggcttcccag atgacggttt
    13381 ggctgccgga aaatgaagcc taccagttgt ggttcagtcc ggatgtgggc ttgggcctgg
    13441 agtcgtggac cccttatcgg ccgctgccgg ccacgcgcgg cattcatctg ctgggcctga
    13501 ccaatgccgc gccgcgtgga tttttccgcg tggcgccgta gtggggtggg ggggctattc
    13561 ggccaaaaca aagcgggcgt tgcgccagtg gccgcgccgg gcgttgacgg tggttccgaa
    13621 caaatcaaag aaccagaacg actcaccgcc cgccttggac aagctccccg cgcaataaac
    13681 gggcgtggcg ctgtcgggca aaaagtgcgt gtaggggcgg tggttgtggc cgtgcagcag
    13741 cacaagattt tcccgcccct gcaacacgcg cagcagcgcg cgcgaattgc gcaggccgtg
    13801 aaagtagccg gcttcgtcaa agggatagtg cgtcatcagg aaaatatgcc ggcgcgcggc
    13861 gatttcgggc atgtccagca actgttcgag cgcatcgagt tccagcgccg gcacgcgccc
    13921 gctggaatcc caaaagggca ggcgcgggcg cgcggagttg aaggcgatgg ccacggcgtc
    13981 atcgcccagc cagcgcacca gagggaaacc ggtgggagtg caggcttcgg gcaggtcgga
    14041 gcgcagcagg ggggcgaagt atttgcggtg cagggcgcgc ccgccgtggg tgtaaagatc
    14101 gtggttgccg ggcacggcga gggcgccgcg gcgggcgagg gcgagggttt ccattagccg
    14161 cggcgcgccg gccatgaatt cgccctccaa tccaaagttg agggtatcgc cagtataaag
    14221 cagataatcc accggctcgc gcgtgaggaa gtcgcgcagg ccgttcagct tttgctgggc
    14281 atcgcgatat ttcgccgcgc gccgcaacgc aaaattaatc agcgccaccc cgcgcttggg
    14341 atgaaaaatc tccgtcaggc ggtaagcccc cggcggcacg ggcaggtgga catcgctaaa
    14401 atgcaaaata cgcaaactca tggcgaggac tgtagcccaa cgctgcgcgc ccgcaaagtc
    14461 ggaaaccggc ggccgcgggc ggccggcgcg cgcggaaggt ttccgctttg tggcggcgcg
    14521 gagtgagagt atcttctcgg ccatgaaaga gatcattccg accttgcaag cgtggacggc
    14581 cgcggggttc gccagcgccg aggccatttt ggttagcgcg caacattcgt cgcctcggca
    14641 acccggcgcg cggttggtgg tgaacgagcg cggcgagctg gcgggcgcgg tttcgatggg
    14701 ctgcgtggaa agcgatttgc gcgaacacct cctgcgcctg ctgcgcggcg agtcgcccga
    14761 gcaaatcgtg gcctatggcg cggcattcga gcccgcgctg gaggtggggt tgtcctgtgg
    14821 cggcgaaatc aaagtctggt tgcgcaagca ccatcctgat gccgccgcct ggcgcggcgt
    14881 ggtggcgctg ccggcaacgg cccgcgctct gctgttgacg ccgctggttc ccgatacgcc
    14941 acagattctg ctccatcccg gcgacacgcc gcccgtgccg gcgatgcagc cgatattggc
    15001 tgacttgctc cgccgcggcg ccaacggaaa attcacgctg gagggaacga cgtggttcgc
    15061 cgaggccatc gcgccagaac cgctgctgct gattgtcggg gcttcgccca ttgcggtgga
    15121 gctgtgcgcc atggccgcgc gcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnngccgat atcgcccgca tccgcgggcc catcggcctc aaaacgatgg gcgcggtgga
    15301 gccgccggaa atcgcggtat ccatcctggc gcaactgatc atggcgcggc gtggcacgtt
    15361 gccgcccgca gtagcggagg aacccacgtg attccttggg aacaactgac cggccgacgg
    15421 cccttcaatt gcccgcaatg tggcggcgaa ctggcggcgg atgacgtcaa cgtggcgcag
    15481 gatgccgcgc tctgccgcgc ctgtggctac caaggcgcat tcctcaacgc gctgctggtt
    15541 cccggcttga gcgatcacga tttgacccac ccgcccaagc gcgtacaact cacgcgcggt
    15601 ttcggcgata cgctggtcat tacatgcttc ccggcgcgcg gcacgctggc gttcctgatt
    15661 cccttcacgc tcttttggtc gggcatcagc atctgggggc ttatcgttcg gccgttgctc
    15721 gcgcccgagg ccatggaatg gcggcaaatc ctgtttgccc tgccttttct gctgggtacg
    15781 cttttgctgc tggccaacat cgccttcctc atcgccgggc gcacggtgct gaccgtcagc
    15841 aaaaaccaat tggaattgta tatgggggcg ttcaacattg gccgccgaaa aatattggcg
    15901 cgcagtccgg agatgaccgt cacgctggcg aaatccaact atcaggtgaa caaagtgcgc
    15961 cagccggaga ttgtggtgac gagccagggc cgcacgctgc gttttggcgc catgggcatt
    16021 cccaacgatg tgctgccgta tgtcgccgcg gtcttgcgtc gaaccctcgc cggtggctaa
    16081 agtaggggct gtgagttcca tcgccgacaa tctggagaac atccgtgccc gcgtggcggc
    16141 ggcttgcgcc gcggcgggac ggtcggcaac ggatgtccgc ttgctggcgg tatctaaaac
    16201 ctttgggccg gaggccatcc gcgaagccgc cgctgccggc cagcggctct tcggcgagaa
    16261 ccgcgtgcag gaagccgccg ccaagattcc ggagtgcccc ggacgactgg aatggcactt
    16321 ggtcggccac ctgcaaagca acaaggcatt tgtcgcggcc aacctattcg actggatcca
    16381 ctcggtggat tccatcaaat tgctcgacac cctcgaccgc cacgcggaga cggtggggcg
    16441 acggctgaac gtgttgctgc aagtgaatgt ctccggcgag cgcgcaaaat ccggcctgcc
    16501 gcccgcggag gtgccggcgg tgctggcacg gattccccac ctgcggaatg tgactgtgca
    16561 tggcttcatg accatcccgc cgctgacggc ggaccctgaa aaagcgcgcc cccattttcg
    16621 acaactgcgc gaattgcgcg accaatggac gcgcgacctc gggctggatt tgcccgagct
    16681 atccatgggc atgacgcacg acatggacat tgcgattgcg gagggcgcca ccatcgtgcg
    16741 cgtgggcacc ggaatttttg gcgatcggcc gcggccggcc gccccggcgg aggaatcatg
    16801 agaaccgaac agattgcatt tatcggcggc ggcaacatgg ccgaggccat tctgggcggc
    16861 ctgttggcca gcggcgtttc cacgccacag cgcctgcgcg tggcggacat tgcgccggaa
    16921 cgccgtgcgt ggctggcgga aacgtatgca gtgaccgtca ccgacgacgc cgcggaagcc
    16981 gtgcgtgatg ccgccgtagt gattttggca gtgaagcctc agcaagccgc ggaagtgctt
    17041 agcgcggtgg ctccggcgct ctcgcccgcg caactgatgg tttccatttg cgccgggctg
    17101 tccaccacca cgctggagtc gtgggtgccc gcccgcgtgg cgcgcgtgat gccgaacctg
    17161 ccggcgctgg tgggcaaggg ggtgtccgcc atttgcggcg gcagccgcgc caccgccgcc
    17221 gacttggatt tggtggccga gttgttcacc gccgtgggcc tcgtggtgcg cctgccggaa
    17281 agccagatga acgaagtgac cgcgctgagc ggtagcgggc cgggctatgt gttcgcgttc
    17341 attgaagccc tcgccaccac gggcgtgcaa atgggcctgc cggaagatgc ggcttgggcc
    17401 atggctcgcg aaaccgtgcg cggcgcggcc gaattggccg cacatacggg cgagcatccc
    17461 gccgacctgc gccgcaaggt atgctcgccg ggtggcacca cgctggccgg tttggccgcg
    17521 atggaggccg ccggcttttc ggcagcggtg gcggccggga tgcaagcggc gcggcagcgt
    17581 gccgcagaat tggcgggggc gtcatgaccg acaaccctcc cgctgccggt cgcccgcgaa
    17641 gccttgcccg gcctctggtt cgcttcgtcg tctggctcac gctcaccatg gcgttgatct
    17701 acggcgcctt gcaattgctg gtgcgcaccg acttcttccg ctcgctggtg gaaaaagaac
    17761 tctccgacct ggctggcatg gaaatccgcg ttgggcgtat ccgcgcgacg gaatccctga
    17821 acctgcgcct gcgcgacacc atcggggtca gcgatgtggc gggcatcgag gcgcgcttgg
    17881 cgcggattcg ctggcggcta tttcgccccc gcggcgaacc catgctggag tcgctgcggg
    17941 tggatggact ctcgctgacc atcgcgcccg atgaatcggg ccggctgcaa ccgcagtttc
    18001 tcggcgaact ttcccggacc attttccaat ggaccggctt gccgccaccg gcggcgctgg
    18061 ccgagctggc tgcgccggca gcaaccactg caacccccgt acgcgcgacc ggcttggcct
    18121 cgcccgaaca atggctgcgc gggccgctgg ctttgcgcgg cgtcacggta cgttggcagg
    18181 atgccaaggg caaggtgctc gccacggcgt cggggctgga tctcatctgg acggcgctgg
    18241 tcacccccaa cgggcggcgc gtctcgcact tggaatgcaa cgcgggcgat ttgcaattga
    18301 cccgcggacc gcacatccgt gacttgcacg tggaattggt gggcgtgaac ggcaaacgct
    18361 ttttggtgga actgaccgcc gccgattggg gcggggccac gccgccgcca ccccccggcg
    18421 cggaagcccg cgccctgctg gattcgctgg attcccccgc gccctgaccc gcctgccgct
    18481 tacaaaaact acaaattttt ccacggcttg ccgcccgctg aaaagccgcg cccggggggg
    18541 attgcatttt tattgaggat ggggtagaac accccgcgac gattcacttg gtggatatag
    18601 ctcaacttgg tagagcacca gattgtggtt ctggctgttg cgggttcaaa tcccgttatc
    18661 caccccactt cactccgcac ccatcccgcc gcattgttat gctgcggcca tggtcaagga
    18721 tcagggcagg acgatgacgg ggagatggtc gcggacggca tggacgagtt tcagcgcgtc
    18781 ccagttggtc aaccggaagc agccgtgcga ctcggttttg ctaatgtctt cgggggcggg
    18841 gctgccgtgg atgccgtagc cggggcggtc cagcccgatc cacgccaagc ccaccggatt
    18901 gttggggcct ggcggaatcc gcagtttttt gccgatggcg gccgctgccg gatcttccgc
    18961 gaacagcgcg ggatcaaacg tgtattccgg attttccgcc cacaccacca cgcgcagcgt
    19021 ttcgttgatg ggtcgcttgg ctttgtcggc cgcgatggaa caggggaagt gcgccaccag
    19081 caggccgttg gtgccgtagg cgcgcagcgt tttttgcgcg atgcgaattt caattttcgc
    19141 gaggcgcggc agcggcttgg cggtcacggc gggcacggtg acggcggagc cggcgggtgg
    19201 gttgggccag gcggcctgcg gattgagctg gcgcaaggtg gcgcggtaca gatgaaactt
    19261 ctcggccacc atttcctcga tgctggaata gcccatgcgt tcctggcgtg agcgctccag
    19321 ccaggaagaa ggataaggcg tgagcgcggc gtggtccgcc gctgtgatga cgtaggtggt
    19381 gaataatccg ttggtctgtc ccaaggcggc cagtgcggtg tcgtccacgc ggccggtgat
    19441 ggggtggcca tgtttcttct gccaggcggc gagggcgttt tcgctcttgg ttccccagcg
    19501 gccatccaaa ccgccgggcg aaaaattttc ggcatcgagc cgcatttgaa tcgccagcaa
    19561 agccatttgc ggtatggtga ttgtcggctg gggtggggcg ggaggcggtt gcgtgactgg
    19621 ccccggcggc tgcggacggg gggcgggggg gcgtggcgcg ggcgatggca cgggccgcgg
    19681 cgcgggctgc atggttggct ccggcaatgg ggttagcggc gcgttggata catgcacgca
    19741 acctgccagc gcggcgcaaa gaaacgcgcc ggccaacgcc caaagcccac gttgaattat
    19801 gacacgcatg gcgggaatat ggcgaaggcc ttcgccggga gcaaccgcaa agcgctaatt
    19861 aaaaaagaat cctgttgttt ccgtggacgg gcatggcact atgccccgcg agtgcgcggc
    19921 gtcgggccgc gaccaacccg cgaggaggct gtcatggctg aacatttttc cgtccccccg
    19981 gtgaggccga caacatcggc cgcgtctccg cccactgccc gcctgcccaa acccaactgg
    20041 ggcactttgc tggtgccgtt gttgctgctg gcggcgctgt tgatcctgcc gctgtgggtg
    20101 tggacgtttt gccgcatcga acccggcccc ggcgaaattg ccatcctgat tcgcaagacg
    20161 ggaaccgatt tgcccacggg ccagattctg gcgatggagc ccggccagaa aggcatccaa
    20221 ctggatgtgc tggccgaagg gcgctacttc cgcaatccgt atcactgggg ttggcagatt
    20281 gccaagatca ccgacattcc gccgggcaag ctcggcgtgc gcacccggct gttcgggcgc
    20341 gatctggccg atggccaaat cctcgcgccg gagggctaca agggcatcat ggccgaagtg
    20401 ctcggccccg gcaaacaccg gctgaatccc tacgcctaca acgtccagct atttgacgcc
    20461 attaccatcc gccctggctt tggcggcgtg gtgacggcgt tgaccggcca agatgtgctg
    20521 gccaatgacc tgcccgaaaa agagcgcaac acgtttttgg tgagcgccgg cctgaagggc
    20581 gtgcgcccgg atattctgga tgccggcacc tactacctga atccctacat ggtcaacgtg
    20641 gtggaggtga acctgcaaag ccagcgcttc gagttgagcg gcgaggatgc cattagtttc
    20701 ctaaccgtgg acggcttcac ggtcaacgtg gagggcacca tcgaatacgc gctggtgcgc
    20761 gaccacgtgg cgcaactgac tcaccaagtg ggcgacatgg atgacatcct caaaaaaatc
    20821 gtcctgccca aggcacgcgg attcagccgg atggagggca gcaaaaaccc agccaagaat
    20881 tatattgccg gcgccacgcg acagaagttc caagacagcc tcgaagccca tttgaaaacg
    20941 cagtgcctgc cgtggggcgt agatatcaag tcggtgttga tccgcaacat cacgccgccg
    21001 gatgccattg cccggatcat ccgcgagcgc gaggtggccg tgcaggatgc caacaaatac
    21061 ggcgaacaaa tcgagcaggc caaatccgag gccgagctgg tcaagcagga gatgctggcc
    21121 cagcaaaaca aggagaaagt ggaagctgat acgcggcgca tccgcgcggt gattgcggcc
    21181 gaacaggaac gcgaagtccg cctgacccgc gcgaaccgcg acctggaagt ggccaagctg
    21241 gaaaacgacg ccgcccgcgc ccaagccgat gcccgcctcg tgcgggcgca ggccgaggcc
    21301 gaagtgatcc gcctgcgcaa cgaggcggaa gccggcgtgc tagcaggcca agtcaaggcc
    21361 tacggcactg gcatcaatct cgcgcgcaat ttgttctaca ccaagatcgg gccgcaaatc
    21421 cgcgcgattt tgagcggcga ccaaggcgag gggctcggcg ggctgctgcg cggatatgtg
    21481 ccggcagccg cgaaaggagg tgcccaatga aaaagagcgt gtcggccatt gtggtgttgg
    21541 tggcgttggt ggcgggtctg tggctgctgt gggagtgggg tttctgccgc ttttatgtgg
    21601 ggcccgatga aatggcgatc atcaccgcca aaaccggcga gaacttgccg ccgggccaaa
    21661 tcctcgccaa accggggcaa cggggcattc tggaagatgt gctcggcgaa ggccgccact
    21721 tccggaatcc gtggttgttt gaccacgaaa tccggccggt gacgtttatt ccgccggggc
    21781 gggtgggcat cgtgaccgcc aaggtgggcg aagacttgcc gcccggcgaa ttcttggcgg
    21841 aaaagggcca gaagggcatt tggcgcaatg tactcggccc cggcaaatac cggctcaatc
    21901 ccgtgggcta tcagattgat gtggtggatg ccatcagcat tccgatcggc tatgacggcg
    21961 tggtgacgtc gctctcgggc gtgcaaacgc agcccaacga gtttgcgcag cgcggccaga
    22021 aaggcgtgcg gcgcgatgtg ctgcaaccgg ggctctactt tgtgaacccg cgggaataca
    22081 aggtggatgt gctggaaatc ggcgtgaacc aggtgtcgct gatgggcaag ggcggcggcg
    22141 cggtgatcac caaggcggtg cagacctcgc aaaacgtggc gatggaccgg ctgcaaagca
    22201 acctgctgca agagcagcaa gccaagcgcg agaattatat tcgctcagag gccagcagcg
    22261 ggatgcgctt gcgcgcggca gcaccgtcgc cggccaagtc aggtttatct tcattagggg
    22321 ggcgggcggt agaaaggcag gccgccatgg acgacaaagt tatgccggcc gcgccgctgg
    22381 agatggagat gccttcgctg tcgtttgtgc tggagcaatt cgtggagttc ccttcgcgcg
    22441 acggctttga aatcagcctc gacatgaccg tggagtttga attgcaaccc aagaacgtgg
    22501 cttggatttt ccgcagttat ggcgacctgc cggccgcggt ggacaaggtg atcatgccgc
    22561 agatcctgtc ggtttcgcgt ttgaaggggt cggcctatgg cgccaaggac ttcatcatgg
    22621 gcgaaggccg cgaaaagttc caaagcgagc tgaccgaagc gctggccaaa acgctggcgg
    22681 aaaaacaaat catgattcac gccgcgctca tccgccacgt gaatgtcccc ggacagattt
    22741 tggacccgat ccagcaggcg agtctcgcgc aggagcagaa tctgaccaac aaggaaaagc
    22801 agaataccgc gcgcaagctg gccgagctga atacggaact gagcctgatt gaccagcgtc
    22861 gcgagcaagt ggcgcaggaa accgaaaaaa tcaaggcgct gatccgggcc gatgaagaaa
    22921 agcaagtggc cgaaatccaa ggcgacacca tgcgccagac cgccgaaatc gaaaagcaaa
    22981 ccgccggcgt gcgcgccgag cgcctgacca cgctgggcgc ggccgaggcg caggtggtca
    23041 cgctggtgga tggcgaaaag gccaagggcg aacagttgaa agtgaaggcg tttggcgatg
    23101 ccgaggccta taacctgtgg gtcctggctg cggcgctttc cgacaagctg caaatcaaca
    23161 ttctgcatac cgggccgggt acgctgtgga cggacttgga gaaagccggc ctggcggaga
    23221 tcggtgcggg cagcgccgtg cagcaaaagc agaaataagc cggctcatgg cggacgcgcg
    23281 gctcacgcgg ctattgcagg aaatcacggc ggaggcacaa ctcaccgcgg cttataccgg
    23341 gcgggccgcc tttcgcccgg ccaccatggc ggctctggcc gacacgccgc gccatgagtt
    23401 cgtgcgggcc gccgagcagc tggccgccta tctggacatg ccgttgccaa ttggccacgg
    23461 ccagaccatt tcgcagcctt atatcgtggc gttgatgacc gacctgctgg atttgccgcc
    23521 ggcggccaag gtgctggagg tcggcgcggg cagcggctat caggcggcgg tgttgtcccg
    23581 cttggctcgc caagtccaca ccatcgaaat tgtgccggcg ctggcgatag cctgccggga
    23641 acggctggca cggctgggct atgccaatgt ggtggtgcac gagggcgatg gggggctggg
    23701 cctgctggcg gaggcgccat ttgaaggcat catggtgacc gccgccgccc cgcgcatccc
    23761 gccggcactg gtggatcaac tggcagcggg gggacggctg gtcattcccg tcggcgcggt
    23821 ggatgccgtg caagagctgg tggtggtgac caaggacgcg cgcggcgcaa caaactcccg
    23881 cacggtgctg agcgtgcggt ttgtgccatt gaccggccaa gccggctaaa aggtgaagag
    23941 taatccggcg ttcaccatca ggccggacat atccaagttc acgcggatat cgtgcagccc
    24001 ccattcatcg cgcgaacggg cgggtgtgcg cacggtggtg tccacccaca catatttgcc
    24061 ttcgcagtaa agcgcgaggt taccggtcaa ttgcatcttg aggccgccga gtaaataacc
    24121 gccggggttg tcggccattt ccacgtcata aatcgtggtg gctttgccgc gtttgcgcgt
    24181 gaattggccg tcgaacaggt aatagcccac gccgggcccg ccatacaccg acaccttctg
    24241 gcccattggc aaaatcccca ccacgcccag ctcgaagggc atggccacga gcgtgaagtc
    24301 atcgtcggac caaccgaggc cttcctcata cacatcacgc gagaacccgg aggcgtagcc
    24361 ggagaggcgc aaatccaatg ccaaatgctt gtgcagccgc acttggccga tcacaccgcc
    24421 gcccactgcg ccggcgatgt caaaatcatg caaatccgcg gcatcccaga agctgaagtg
    24481 aacgccaagg ctgaagcagg tcaacggccg acaaaccggc gcggccatgg tcggctcttc
    24541 ggtttgcgcc atcgctccca ccgccaaact caacagcatc cagctcacta gcaccatgcg
    24601 tttcatcggt ttctcctgcg gggccacggc ggccccatcg gcaacatacg cacgaactcc
    24661 gccgccgcaa agccgaaacc gtccgcagcc gcgctttgtg ctttgtctgg gcagcagtcc
    24721 tgtgctacgg tcgctccaac acacgtgagg ttgactatgt atccagcacc gccccgccgc
    24781 tgccgccatt ggcccttttg ggttggcatt ttgatgttgg gtttcgtgct ggggttgagc
    24841 ctgctgctaa atctggcggc ggtcggtatt ctggtggaag gcgcggagag caccggcggg
    24901 gaaggcgaag acgaataccc gcaatttacg gaaacgcatt cgtatggcgc gggcacgacc
    24961 aaagtggtgc gcattggttt tagcggcgtg ctaacgcgcg aactggatgg cggttggctg
    25021 ggctcttcgg acccgattga agaatgtcag cggcagattc gcgccgcccg tcaggacaaa
    25081 aaggtggcgg cgattatttt cgaagtgaat tcccccggtg gtgaagtcac tgcggccgat
    25141 gacatttacc gcgagttgac gctgttcaag gaaagccgtg aagaccgcaa gctggtggtg
    25201 ctggtgcgcg actacgcggc ttctggcggc tactacattt cgctgccggc ggatcacatc
    25261 gttgcacagc ccaccgccac gatcggttcc atcggcgtgc tgctgcagac ctacaatgtg
    25321 cgcggcctga gcgagaaact tggcgtcacg gatacgacca tcaagtccgg ccgcaacaag
    25381 gacatgctca atccgttcca gccggtggac cccgaacagg tggcgctgct gcaaggcaac
    25441 gtggatgcca tgtatgagcg ctttttgggg ctggtggcca ctgcgcgcgg cgtgacgaaa
    25501 aacaaactgc gcccgctggc cgatggccgc atcttcacgg cggatgaagc gctgaagcac
    25561 aagttgattg acagcatcgg ctactgggat gaagcctgcg caaccaccgc tgacctgctc
    25621 ggcgtggagg aattgcgcgt ggtggcctac gaatcgcgcc aatcactttt taacatgctt
    25681 ttcagtgcgc ggatgccgct gcccaactgg cgggcctggt tgcaccgcgc ggccacgcca
    25741 cggattcaat acctctggca gtactaatcg cttccgttcc gccgccgagg cgagggggga
    25801 ggggtcagcg cttttcggtg cggacggcgg caacaaattc cgacatgcgc ttcatggctt
    25861 cgcggatgtt atccatgctc gtcgcgtagg cacagcgcag gaaacccgcg ccgcattgac
    25921 cgaacgcatc gcccggcacc accgccacgc ctttttcttc taggaagcgc aacgcgaaat
    25981 cttcggcgga gatgccaaga tcgccgatgt aggggaacgc ataaaacgca ccagccggtg
    26041 tgtggcaggg cacgcccatt tccgcaaacg aggcaatcag gtaattgcgc cgccggttgt
    26101 actccgccac catgtcggcc acggcggaat cgccgcgctc caacgcctcg gccgcggcct
    26161 tctggccggt ggtcggtgcg ctcatgatgg cgtattggtg cagcttcatc atattctcgg
    26221 aaatctccgc cggggcgcag gcatagccga tgcggaaacc ggtcatggcc cacgccttgg
    26281 acattccgtg caggaaaatc gtgcgctcct tcatggcggg tatggaaaca aaactgacgt
    26341 gcttggagtc gtaggtcagc tcggcgtaga tttcatcggt cagcagcagc agatcatgct
    26401 gcacgcaaaa cgccgcaata tcctccacat cctgcttggc cagcacggcc cccgtcgggt
    26461 tgcaggggaa gttcagcatc agcacgcgcg tcttgggggt gacggctttt tccagcatgg
    26521 cgcgggtcag gcgaaactcg tcttcgcgcc gggtttccac gcaaatgggc acgccgccgg
    26581 ccagaatgat gctgggccgg tagctgacga agcatggctc gtggtagagc acttcatcgc
    26641 ccgggttgat gatggtgcgc agggccacgt caatggcctc gctcacgccc acggtcacga
    26701 gaacttccga ttcccagtca tattccgcgc cgaactgccg cgcgacatag cgacagatgg
    26761 ccttgcgcag cgcggggtcg ccgcggttgg aggtgtagtg cgtggcgccg cgctccagcg
    26821 agaacaccgc cgcctcgcgc actgtccaag gggtatcgaa atccggctcg cccacgccca
    26881 gcgaaatcac gtccttgcgc tgcgcgacga tgtcaaaaaa cttgcggatg cccgaaaacg
    26941 ggagttgccg gacgtgctcc gccactcggc tgcgtgtttt catggtttta tagtatgtag
    27001 agaattacgg gctgatctgg aggcgctcat ccaccttgtc gccatccatc agcacaccgt
    27061 cttgcttgta ggttttgagc atgaaatgcg tgccagtgga cagcacgcca tccagcgtgg
    27121 aaagtcgttc gctgacgaac gaggccactt cgcgcaggtt tttcccctcc acgaacagga
    27181 gcaggtcata cttgcccgac atcaggtaca tgtttaccac ttcggggaag ccgctgatgc
    27241 gccgcgcaat gcggtcgaag ccgccttcgc gttccggctg cacgcgcact tcgatcactg
    27301 ccgtcacgtt gtcctgttgc agcaaatcct cgttcaccag cgcgcggtag ccacggatgg
    27361 tgccattttt ttcgtactcc gcgatccggg ccgccacggc ttccggggtc gagcgcagca
    27421 tccgggcgat gtcttccaag ctcgtccgtg cgttttcttg caatatggtc aacagttcat
    27481 ccatgactac ctcccgccgg cacagccggc tgaaagcgcg aaccctacca agccgtcccc
    27541 cccaacgcca atgaaaagat caggcaattt tggctggtta ttggccggcc taatccagca
    27601 gcgcgccgcg cggtccggcc ttgtcgtccc agttgcgcgg cttttgcgcg ctgatggcgg
    27661 caattttttt ggtggtcatc tggatgccgc gcgttttggg cgtgcggatg gcgactttct
    27721 tgggatcgaa ctcctgctga tggatttgct gtcccttgcg cggcgcgtat ttcacgtaga
    27781 gcgttgcggg ttgggcatcg gagaaaaaat ggcagatggc cggctcgccc tcgggcgcgc
    27841 aaacgtattc cttgttctgg atcacgccgc cgaacgtgaa gcgcttgagg tggctgatgc
    27901 cgtcggcctc gtacaccagc gtcatgacgc ggtcgcggtc atagatggcg gcatagacga
    27961 ggtcttggtc cacgaacagc ttggcaggcg gggggatcac gcggtagcgg cagtccttcc
    28021 acaccagaat gattttgtcg taggacgagc agcggaagag cggctcgccg ccgcccacgt
    28081 cgtagccgag gtaacctttt tcgcggtcga agaccacggc atgttcttcg gcggtcagct
    28141 cgcgcacttc aatggcgtca aagcgggcct cgcgcgtttt gcgcgggaaa aggtcggcgt
    28201 agtcgcggta aatacccttg aggtaggtga tggtgaagcg ggtgacgtcg gtcaggtgct
    28261 cttccacgga ggcgagatcg gcgagcagat cgtcttcctc tttgcgggac ttgttgatgt
    28321 cgtagagcga aatgcggcgg atcgggatgg ccagcagcat gtcgagatcg gcgttggtga
    28381 tgtcgcgtcg gagctgatcg cggaaggggg cgaggccgtt gaggatggcc tgccggacat
    28441 ccggcgcggt tttgcattcc tcgatgcgct tgtagatgcg gttgacgatg aacagccgca
    28501 cgagggaagc cgcgtggagg gcttcatcaa tcttcttgcg ctcgatttgc agttccttac
    28561 gcagcagctc caccagccgc cgggtgttat ggcgcaaaat ctcgtgtacg gagagctcca
    28621 ccgggcgctg ctcatggatc acgatgggcc gcagcgacag cgacacctcg cactgggtga
    28681 acacatagag cgactgaatc gcctgctgga tgtcggtatc cgccgccagc gcgatttcga
    28741 tttccacctt ttcggcggtg tagtcgcgga tggccttggt cttgatttta cccttcttgg
    28801 cggcatcctc gatggagccg atcagcgctt cggtggtgac accgaagggc aactcgcgga
    28861 tgaccagcgt ggcctcgtcc ttttggtcaa tcacggcccg gcaacgcacc ttgccacggc
    28921 cttcgtcata ttcgcgggca tccacgagac cgccctgctg aaaatcgggc agcagcttga
    28981 acggcttttt ctgcagaatg gcaatttgcg cctggatcaa ctcgccgaaa ttatgtggca
    29041 ggatgcgcgt ggacaggcca acggcaatgc cctcggcgcc gagcatcagc agcagcggca
    29101 gcttggcggg cagtaccacc ggttccttgt tgcggccgtc gtaggagggg atgaactcgg
    29161 tgaggtcgtc gttgaacaac tgcgtggcgg ccagatcggt cagccggcat tcgatgtagc
    29221 gcggcgcggc ggccggatcg ccggtgtgga tgttgccgaa gttgccctgg ccctcgatca
    29281 ggtaaccgcg gttgaccagc gtgaccaacg catcgccaat cgaggcatcg ccatgcgggt
    29341 gatactgcat ggtatggccg acaatgttgg ccaccttgat gaagcgtcca tcgtcttttt
    29401 cgtgcagtgc gtggagaatg cggcgttgga cgggcttgag gccatcggcc agctcgggga
    29461 tggcgcggtc gcggatgacg taggaagcat actgcaaaaa attgccgtcc atcagccggc
    29521 gcaacgggcc gtcgccgatg tcggaatcgc tgacgggctg cttgctggcg gcggggatgc
    29581 tggggggcgt gcttttgccc cgcgcggggc gaggggcggg ctcctcgtcg tcgtcaaaaa
    29641 gggaagattg tcgggtgtcg gtcatggggt caacgagcgg ccactatacc cacgcgccgg
    29701 agggcgcaca atagccaatc tcgaccgcgt ggcggcgcgg acgcggtgcg gactttgcgc
    29761 ggcgcgggcg gtgtgctaca accgttccac actgtggaaa aaagttttcc acagtgtgga
    29821 aaagtttttt ccacggcgtg gaaaacgtga gcgaagaaag gttgatgtga tgagcaagca
    29881 gaaggcccag gcgccggagg tgatcagcaa agcggcggcg gcccgcaaga ttgtggcggt
    29941 ggcgcaggag atccatgacg acatcgtcaa aaagcgcaaa aagccggcca tgagctttcc
    30001 ggtgcgctcg ctggcgaacg tgaaatatga cgccaagcgc gggcattttg aaatcctggg
    30061 caagcgggcc acgcgcacgc tctcctacaa cacggtcaag gccttcgcgc aatccatgcg
    30121 gctgctggcc accaccaaga atgacctgct cgatcgcgac gacatcgccg gcaagcgcga
    30181 ggtttattac aacagcaagg ggtggggcga atgccgcttc gacgaacagc ccgaaagcga
    30241 cgcgctgctg gatgacatgg aggccatgct cggcgtgaac cgcgagcaac tgggctacat
    30301 ccccgaagag cgcggcggcg atgtctgcgg cgagctgacg gtgattgacc aggacccgca
    30361 aaccggcgcg gacgtgaaaa tcaattgcgc caagctgggc agcggggcgt ggagcattcc
    30421 gtcgcgcgtg gagcacctgc gttttgaaac gcgcgccaag ctggtgctgg tggtggaaac
    30481 ggcttcgctg tttcagcgcc tcgtgcacca ccgctatttc gagcgcgcca actgcgtgct
    30541 gatttccatg agcggcgtgc ccacgcgcgc ctgccggcgc tttattcgcc ggctggcgga
    30601 tgaccgcaat ctgccggtgc tggctttcac cgatggcgac ccctacggct attgcaacat
    30661 ttaccgcacg ctgaaggtgg gctccgggca ggccgcgcac atcaaccggt ttttctgcgt
    30721 gccgcaggcg cattatctgg gcgtgactcc gcaggacatc ctcgattacc agctcgaaga
    30781 cgccacgcac ccgctcgaag aggtggacat caaacgcgcc aaggacgcgt tgaaaaacga
    30841 tccgttcatc cgtcaccata aagagtggca gcaggtgctg gagcagatgc tgcagatggg
    30901 tgtgcgcatc gagcagcagg catttgccaa gcacgggctc aacttcgtgc tggataccta
    30961 cctgccggaa aaaataaaaa aaggaaagtt cctgccgtaa acggccgctt tcccgatttc
    31021 aatggtggat ttggaagcgc caatggtcat tgaccgtgag agcgccataa tgccgtcagg
    31081 gaagcaactg aagaacaagg ccaagagcaa gcgaggctcc ggtgcccacg gccggttgat
    31141 cccctaagcc ttccttggac tgccatgcag gttcgcaccg ggcagataaa acacaaaaat
    31201 gactcatctt aaaagtgtcc agagtttata aagtctggac attgccttca aaacggccaa
    31261 ttaaaaaagc tcgataaaat atacgcatca aattgcatat gattgagatg tgaatatttc
    31321 aaaaaatgcc cacagtttat aaactctgga cagcatggtt aaaatagcaa aaaagaagtt
    31381 ggtttgccgg tggccgtttt ctcatcacaa aacggacaat ccagccactg aaaaaagcga
    31441 ggaaccggta aacggcaggc ggggagtcag gagtccagcg gcaggttggc ttggagcccg
    31501 ccgggcagcg cgtagcggtt gggcgcggtt ttgcggatga gcgggtcgtt cagcagcacg
    31561 gcgcggagga agccactgcc gcgctggcaa gccggtggca ggcccgcgtt gaaagcggcc
    31621 accaaagccc gcagcgacat ctcgccattg gccgcgagga cttcgcgcag cagttcgggg
    31681 gtgtcgcgcg cggccagtcc ggcgcggtcg tccaatgtga gcagggtatc ggtgcggtgg
    31741 cgcagcaggg cgcgcagcaa tggggcgaaa tcgctggcgc cggcggggcg cgcggccttg
    31801 ggcaggcgcg cggccagcgt ggccaacgac tgcaagccgg gtgcggcgac cagtcggtcc
    31861 accagcgctt tttccgcgcg ctcgatggcg gtggcggcat aaaggctgaa gaaatcgcgg
    31921 taacggatca ggcggccggg caccagttgc accagcagca gaaacgcacc caccggcgac
    31981 gcgtcgcccc agatgggcgc agcttgcgcg gcgacttccg ccggctcaag cacgcccccg
    32041 cccgcttgca gcgtgacggt ggcggcgcga taaaacggct cggccgcttg caggggcaac
    32101 gcttgccgca atgtgcccaa cgccaagttg agcagttgtc gcacgcgctc ccgggtgacg
    32161 cccagtttga agccggtttc gcgcaggcgc atctcgtgtc gcgtcaaact ggcggtgggg
    32221 tcgtcgaagg caaaatgcag ttggatggca tcagcctgcc gcgcgggcag aaacagcgcc
    32281 agccactcac gcagattcag ggccgggggg cgctgggcgc aggtcgcggc ggcataggcg
    32341 gcgatgcgcg cgagcaaggc gcggtcgtcg gcatcgagtt tatggtcggt gggcagcggc
    32401 ttgcgcaggt cggccaaggt aatgaatccg tgggctcgca gcacggccag aatacgcgcg
    32461 ggcaggccgc tggccggcag cgcggccttg gcgaggctcg cgccgggtgc tggagtgggg
    32521 gggctcacag gttgggggcg gtggcggctt gcaacagcgc ttcgtattct tcggcagtca
    32581 gcagtttttg cgcgagcgcc agttccttga gcgtctggcc ggtggcgagg ctttcttgca
    32641 ctagcttggt cacgcggtca tagccgaggc gcggattcag cagcgtggcc agcgccgcgc
    32701 tgcgctccag gttgcggcgg cacgcggcgg catcggccgt gattccagcc acgcattttt
    32761 cggcgagggt gcgcgcggcg ttggtcagca actgggcggc ttgcagcgtg tgcgcgccga
    32821 tcagtggcat gtgggtgttc agttccagtt ggcccgcgcc gcaagcgagc gccacggcgt
    32881 gatccaaccc ttgcacatgc aggcagacca tgttgacggc ctcgcaaatg gatggattga
    32941 ttttgccggg catgatgctg gagccgggct ccaccggcgg caggcggatt tcgcccaggc
    33001 cggtattggg cccggaggcc agcagccgca ggttgttggt gatttggttc agatcgaacg
    33061 cggcgttgcg cagcgcggcg gaaacatggg caaagctact catgaattgc gtggcgtgga
    33121 tgccatccgc cgcgacgcgg aatgggtgag ggctttcgcc ttgcagcgcg cggataattt
    33181 cggcgcggaa ggccggcttg gtattgatgc cggtgccgat ggcgttgccg cccgcgccca
    33241 gctccagcag ctcgtccgcg gcttggcgaa tccgcgccgt ggcggcgcga atggcgctgg
    33301 cgtaggccgc gaattcctgc cccagcgtga tgggcacggc gtcttgcagg tgcgtgcgcc
    33361 cggctttcag gatgggcgca aactccgttg ccctggcttg cagcgcctgc gccagcgcat
    33421 tcagcgcgtc aatcagcgcg ggcatctggt tcagtacggc aattttcgcg ccggacggaa
    33481 aactgttatt ggtggattgc cccatgttga catcgtcatt cggatgcacg ggggccttgg
    33541 cgccgcgctg cccgcccaag gcttcattgg ccaggttggc aatgacttcg ttgagattca
    33601 tgtgcgacga cgtgcccgaa ccggcttgaa aaaggtccac cggaaattcg gcatcgtgcg
    33661 cgcccgtcaa aatggcgcgg caggcggcga tgatggcctg cgctttggtt tccggcagca
    33721 ggccaagcgc ggcgttggct ttggcggcgg ccagcttgag cagcgccatg ccgtggatga
    33781 tttcgcgcgg caacggggcg ccggacagcg gaaagttttc gagggagcgc gaggtttgcg
    33841 cgccgtagag cgcggtggcc ggcaccggcc gcgggccgag ggaatcgtgt tcaagacggg
    33901 tgggcgtggt catgggacaa acatagcgga agcggccaag gggtgacaat ccgttctttt
    33961 cccgcggcag tgaaccgcgc tagcatgggc gccatgtgga aaaagcggca catggtgtat
    34021 tacgtctcgg cgcacggcta cggccacggg gtgcgcgcga gtgatctgat cggcggccta
    34081 ctggacgtgg cgcccgagtt gcgcctgacg gtgatcagcg atttgccgga agatttcttc
    34141 cggagccgcc taccggcggc tgacgggcgc gtggcgttcg cgccgggcgc gttcgatgtc
    34201 ggcatggtgc agcgggattc catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34321 nnnttgtcct tcaccacgct ggggtgggat gcggacgcgc tgcgtgcggt gggggggctg
    34381 gcggagtacg agtttttcac agtgaagccc ctggcgtggc gcgggcagcc caacctgcac
    34441 gcggtggacc ggcacgcatt cagtttttcg gatgtgctgg ccagcgtgga tttcgtcatc
    34501 accaagccgg gctacggaat tttgagcgac tgcgtggcca accgcaagcc catcatctat
    34561 gccgaacgcg aagactttat tgagtatccg ctgctggtgc aggaactgcg gcgttatttg
    34621 gcccacgtgc acattccggc ggcggatttg tacgcgggac atttggcggc agcgctgcgt
    34681 gcggcggaaa ccgcgccccc cccgccggag cagttggcct ttggcggtgc gcgcgtggcg
    34741 gcggaattgc tgctgaacgt gggctgaaac gcgcgcgcta cggcgccacg ctttcgcttg
    34801 caatttcctg aatctgggtg ggcaccagca ccggtgcggc cgcgccttga atccaatatt
    34861 ggcggccttc cagtcgcacc tgcttgtttt gcaggctgcg gaactttgtg gcttggccat
    34921 acacatggca caggatgacc cagcggttgt cctgccactg caccacgcgg tagcgagtgg
    34981 gcgcttcgtt gatgagcggc gcggcccgca attcaccgcg gacttccacc atggcgcctt
    35041 ggccgctcaa cggcacaagc ttgagatcgg cgggggcggg cacagggatc gtacggacgg
    35101 catcatcttt ttccggcacg gaggcggcaa tcaccggcgt ggggaggacg gccggcgtct
    35161 cggcggcggg cttagccacc acggcgggag cggcggaatc ggcatcggtg gccggctttt
    35221 tgcggcgggc ggatttcttg gcttccggca cgggctcggg ctcggccgcg gcgaccgtcg
    35281 cggaatcagt ggtcaggact tccacataat cgcggtgaat ccacacccgg gcttcggcgg
    35341 gtggttcgat ttgcaaccaa tcgccctgca cgttgccggt gggcttgacc gtcgcgccca
    35401 gttcgagggt acacagaatg ttgtagttga tgctcggccc ggcgcgcaca ttgacccgct
    35461 tggtgccgat ggtgttggcg ggggtttgca cataggtctt catgatccat gcctcggcgc
    35521 tggtcggcgc ggcgacgcat acccactctt cgccgatttc gtaggcggtc agccggtcat
    35581 cgtagtgcgc ttgcgtgacg acttccgagg tgagcaggtt cttggcccgc aggttgacgg
    35641 gatcgcccgt cacgcgcacg gtggtttgcg ccgacagggg agagacaacg gccagcccgc
    35701 cggcggccag ccacaggaga agcgatgcag tctttttcat ggtgcgggcc agcataggcc
    35761 gggcccgcca aatcggcaat gccgaagtgc cgggtattga ttccccgtcc cgtttggggt
    35821 agaaagaaaa aatatggcaa ctcaaccaaa acacaggggc ttgggccggg gcttggacgc
    35881 gctaatcaag ggcagtggcc cgcgggcggc ggaaaccggc aagccgccgg cgcgcccggc
    35941 cgcacccccg gctaccgcgg cggtgccgcc ggccaaggat caggtgcggc gcgtggcggt
    36001 gcagaaaatc aagcccggcg cgtggcagcc gcggcgcgtg ttcgcggcgg atgctctgca
    36061 agagttggcc gactccatcc gcgaaaaggg cgtgctggaa ccggtctggc tgcgccaagt
    36121 gggggatatg tttgagttga tcgccggcga acgccgcttc cgcgctgcgc agttggcggg
    36181 gctggcggaa atccccgcgc tgctgcacga agtcagcgat gtcgaagcgc gcgaaatggc
    36241 gctgatcgaa aacctgcagc gcgccgattt ggacctgata gaagaggccg agggctatcg
    36301 cgatttgatg caagccgccg ccctgacgca ggaagaagtg gcccggcgcg ttggcaaggc
    36361 gcgcgccacg gtggcgaacg cgctgcgcct gctggatttg tccagcgcca tcaagagcgc
    36421 gctctcggcg ggcacgctct cagcaggtca cgccaaggtg ctgctgggcg tggcggcgcc
    36481 ggaccgcgat ttgctggcca cgcgggtcat cgcgcaggga ctctccgtac gcgcgttgga
    36541 aaaactggtg gcgggcttgg accgcaccgc gccggcgaaa aagaaaacag atcccgccaa
    36601 aaccaagccg gccagcgacc aaagcgcgcg gcagttgaag ttccttgccg accggatgca
    36661 gcaagaatta ggcaccaagg tcaccctgac tccggcccgg gtattgccgg atggcaagcg
    36721 cgccatgggg cagattgtca ttgagtattt cgacaacgaa gatttgtcgc gcctgttgga
    36781 catgctgaat ttgggtgact tggcctgatc ctgcgccacc ggccatgacg gcgtggtaca
    36841 acgcaga
//
LOCUS       DAOPNP010000008        35345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1601_length_35345_cov_8.146840, whole genome shotgun sequence.
ACCESSION   DAOPNP010000008 DAOPNP010000000
VERSION     DAOPNP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 35345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35345
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_35345_cov_8.146840"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..>1847)
                     /gene="yidC"
                     /locus_tag="PKI64_01645"
     CDS             complement(274..>1847)
                     /gene="yidC"
                     /locus_tag="PKI64_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOD99742.1"
                     /translation="KEVPPPPPATQEAPAPEEPAGPEILTVLANDQLELTFSSHGAGI
                     VQAAILKYPTAKNDPTPVTFDFSDQPAAAPRGYPGLRELASFEVTAATADSITYRKTL
                     PGGLVLERIITLGAGYQLQLEDRLRNQSAAPVALHGYGIQAGFMENLPGEEGRQQIPL
                     LGVDTLTGSDSVKYWAGKFDKWFPASGPRQMPLIPGPNHEPTPVDWVAVKNKYFTQIL
                     TPTDGVSGFGRLFVQHGAPVHSSFLGLFPRTSVPIQRVTAYLEMPDYTLPAGETITQH
                     YTFYAGPKVYAALKANGPHQEDILQLGFWRPIGIFILKIMVWFHDHIWPYNYGLAIIL
                     LTFLIRVIFWPLNHKSMVSTRHMQEIQPLITAVREKYKNDPQRQQQEMMALYKEHKIN
                     PMGGCLPMLIQIPVFFALFTVLRGAIELRFSSFLWIADLSTPENLLRNYLPFGLNILP
                     LFMGLTMWLQQKMTPTSDPQQQKMMMMMPIIFTFMFYSFPSGLSLYWSTNQVMMIIQL
                     ARMRKKHPAPSPAKT"
     assembly_gap    1848..1857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1858..2087)
                     /locus_tag="PKI64_01650"
     CDS             complement(<1858..2087)
                     /locus_tag="PKI64_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99743.1"
                     /translation="MNKKDLLPIILIFILFLAYPTIDRKFVAKIFPPKAKPAPVAVEP
                     ATAPALPADTTLAAPPASPRPPPPPPPPPPHTT"
     gene            complement(2080..2328)
                     /gene="yidD"
                     /locus_tag="PKI64_01655"
     CDS             complement(2080..2328)
                     /gene="yidD"
                     /locus_tag="PKI64_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOD99744.1"
                     /translation="MAKLLCALIRLYQKTFRLLLGNCCRFEPSCSNYMLEAIRTHGVG
                     YGVFLGLLRIARCHPWNPGGYDPVPTKSSDVKRETKHE"
     gene            complement(2334..2747)
                     /gene="rnpA"
                     /locus_tag="PKI64_01660"
     CDS             complement(2334..2747)
                     /gene="rnpA"
                     /locus_tag="PKI64_01660"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOD99745.1"
                     /translation="MQPGRDRPTAPPPDAGRAFCVRPTLGRKQRLRRTAAFDEAYAQN
                     QRWHGRHMVLFRRAAPDASCRLGVVASRKVGNAVERARAKRRLREAFRLNRHRLTGAD
                     DIILVARRSILAAPWPAVVEDLLALAAKANLLPPP"
     gene            complement(2784..2930)
                     /gene="rpmH"
                     /locus_tag="PKI64_01665"
     CDS             complement(2784..2930)
                     /gene="rpmH"
                     /locus_tag="PKI64_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOD99746.1"
                     /translation="MQPTYKPSKIKRNRKHGFRARQATANGRKVLSRRRAKGRKSLSI
                     SDRA"
     gene            complement(3184..4197)
                     /locus_tag="PKI64_01670"
     CDS             complement(3184..4197)
                     /locus_tag="PKI64_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOD99747.1"
                     /translation="MSDNNQSQFLLFKADGGAVHVNVRLEGETVWLTQAAMAALFDVA
                     PQNITMHLQNIYADKELSQDSTCKDFLQVQKEGNREVSRRLKHYNLDAIIAVGYRVNS
                     KRATQFRIWATAVLKEYIIKGFALNDERMKTGRNMTYFDELQARIREIRLSERVFYQK
                     IKDIYTTSIDYDPSAEETLRFFKVVQNKLLWAISKQTAAELVARRANAILPFMGMQSY
                     DKKNQRRITQQDAVTAKNYLTETEMKALGLLVEQYLAFAEAQAQQQIAMTMSDWVARL
                     DAILTLNGRELLTHAGSISHALAEEISTKQLAQFRQRLREEERLSSLAELEHDIQASR
                     DDK"
     gene            complement(4352..4825)
                     /gene="rhuM"
                     /locus_tag="PKI64_01675"
                     /pseudo
     CDS             complement(4352..4825)
                     /gene="rhuM"
                     /locus_tag="PKI64_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(4847..5416)
                     /locus_tag="PKI64_01680"
     CDS             complement(4847..5416)
                     /locus_tag="PKI64_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOD99748.1"
                     /translation="MQQAGKKKFWLVKFAPFRTSWDDIVKAGSFTPRGIRCPQARNNL
                     ARMAVGDLALFFHSQEHRCFTGILTVTRAAYPDPTSADPRWLTCDFAPLQTLADPVSL
                     AQIKSNPALANFPLIRQPRVAVLPLTAFECAAILRLASTPFPAVPTAKQKPIATLVDR
                     ILTAKRTNPAADIISIETDLDALVNFRRR"
     gene            complement(5545..6348)
                     /locus_tag="PKI64_01685"
     CDS             complement(5545..6348)
                     /locus_tag="PKI64_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HOD99749.1"
                     /translation="MPQLTNIEKGAFLRLFNDGGYVLDFSTNDFDNFTLDSVGVALCH
                     HYGLSKGKSLTAYANEAEASKVVKLFTDLLAHFEIHCTSRCERDEDIAKLYARCRAII
                     DRFSNVAVPLAGSAHTVAKKFSSDYMSAQIKLMLEMTTQNPTEAIGKAKEFAESCCRT
                     ILEAHSVSIDKIWDMGKLVGETVKLLCLTPQDIPDTSKEAAAIRRLLGNLREIVSNMA
                     TIRNSYGSGHGKSANYRGLEKRHAQLAVGSSITLVQFLWDSHETQSQRR"
     gene            complement(6351..7961)
                     /locus_tag="PKI64_01690"
     CDS             complement(6351..7961)
                     /locus_tag="PKI64_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD99750.1"
                     /translation="MNDTATTISSAVTTEAPFTLPHTQETPLVPSPVATSESTSTTPA
                     PQPLRIKKISINGYRAFPQYRPHNFEVSLGDDGKNLLLYGENGSGKTSLFKALRDLLN
                     RDLKDTAYTSYQNIFDIGKDDNITVELTSGSPPSFSWGVGTVHPSIDKDKTAFNAFTR
                     SCFFLDYRSLLETNFVHKNNANPNLYTLLVGTILKDVMPPGSRTIRESREILSSALDA
                     VRSYGNSLNQRNAVAAAEKMTTALSTLIPNVVVEANIILGKLQPGTIINLTTPTITYS
                     NGTQKKDRKYTGESIELLIELNGKPIPTPQHFLNEARLSAIALSIYLAGAKFSRAGRP
                     GILVLDDVLIGLDLSNRIPLLELLQNDFANWQIFLFTHDHVWYDLARTWVGEKWISKE
                     LFLVSKDTMAFDRPELRDGDLLAKAKGHLDNKDLTAAAVYIRARFETILSRVCQDSGI
                     EIKYKKNPKNISADNLWQGIQSRQRTRVQEGQSDFITPQLMQDVEQVRSNILNKLCHS
                     ASPSFTDTEVQYAFDIITELSRFDFNKR"
     gene            complement(7958..11986)
                     /locus_tag="PKI64_01695"
     CDS             complement(7958..11986)
                     /locus_tag="PKI64_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOD99751.1"
                     /translation="MTLLTIIKESLKAFANQPLAEAATATDFRAAALDFFAVLGYASP
                     RTLDTGGSPDAFLSTFASSSAGGVHATFNPAAALVSHWRSAHLLFQLTDTELSGENSL
                     FKEGFERTRHDSYIFFAIELSGTSYTRTQLATITRQLNRLFPQPVMVLFKTGAAITLA
                     VINRRANLRDSEKDVLGKVTLIRDINTLTPHRGHLDILASLAVPSLSSPMQSGHTPIR
                     SFTALHAAWEELFNVELLRKTAYYAIANWFFWARENITPPPDAPLDADTKKPSLFLIR
                     LLTRILFCWFLKERRLPNGRPLVHEELFSEKHIRHLLHDASPTATTYYTAILQNLFFA
                     TLNTEMDTPESPSNRRFARNSKDDHMVHTLWRHAEQLRSPDALAALLRTIPFLNGGLF
                     ECLDERVQQGNSNFTREIRADGFSSDPAKCPRIPNFLFFGPNQTADLSAATGNSSQTA
                     VPVAPLLTILNHYVFTVAENTPLEEELALDPDLLGNIFENLLASYNPETGTVARNATG
                     SFYTPDYVVTWMVEQALRPTLSAVLPKSKDTKHEAKLTALFSPDPEARPDFSKQETAA
                     LINAIDNLKILDPACGSGAFPMGALHKLTHILAKLDPENALWKQRQLDTASQIADTPA
                     REAALAAIERAFAHDNGDYGRKLYLIENTLYGVDIQPLAVQIAKLRFFITLIVDQPID
                     PALPNYGILPLPNLETKIVAANTIIGLQRGQLLLGSEQVRKLEAQLKTIRHDYFTARS
                     YPRKKALRKQDRELCKQLAAELAATGTMSTSDADRIARWNPYNTNTAADFFDPEWMFG
                     LSEKFDLLIGNPPYVRQEELKPMTALDTEGNAQPLKTILEDQYETYAGTADLFVYFFE
                     RSLQLLKIGGFLSFITSNKYFRSAYGEKLRIYLAHATEPAAILDFGDTNIFTAIAYPA
                     IFVTKKTKDVERKQLPKLRKGESEESFTKRHFKNPERPFPVFTWRLGPEKSTFPQVFD
                     EQHNTLRQRNLNPSGWRLEDEYDIVLFERIKKAGTPLKEYCNGRIYYGIKTGFNEAFV
                     VDEATKNALIAEHPSSKDVVKPYLRGRDVKRWRYDETGLWLLYIPWHFPLHLNRSIRG
                     ASKEAENEFKSKYPAVYRHLSKFKKELSARNKAETGVRYEWYALQRWGADYWTEFSTH
                     KIIMGRFMDKPTYAFDDAHAYINNALHFIAEANKYIVSVLNSPVVWYYLRMTSPDLQG
                     GFIQASVETQGGIPIPSATPDQQQHCETLATAIIALHKPANADFPNRLAVIALFEQWL
                     NGLIYELFFPAELHARNLHLHTLSAPHLSGCSGAVNGDMSNATLSALHTLISDYKHPL
                     RAALNDLQNLEEIQTIEAVDKK"
     gene            complement(12093..12752)
                     /locus_tag="PKI64_01700"
     CDS             complement(12093..12752)
                     /locus_tag="PKI64_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOD99752.1"
                     /translation="MNRDNGQNRQNGNADFADRQDGRALDSDASGRIYGQHSGYRNLK
                     AYQIAELLYAFTCRFCERYIPFSDRHHDQMVQAARSSYQNIAEGSEDSATSKKLEMNL
                     TNVARSSLGELHKDYVKCLERRNLRHWQQGEAEFEEARQLRPKSLSEAAAWVNRKGAA
                     GTPEERAANLGAILSAQAHWLAERLMNRQAQDFEKGGGFSERLHQARTTARTKNTPPS
                     F"
     gene            complement(12749..16093)
                     /locus_tag="PKI64_01705"
     CDS             complement(12749..16093)
                     /locus_tag="PKI64_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOD99753.1"
                     /translation="MKKPSDSPPSSALRDNHARGSVADFLKEKITADSRLSFVSAYFT
                     IYAYDALKEVLDSIGHLDFLFGEPTFVNRLDPSKAEKKAFILDAAGLKLANKLQQKRV
                     AKECADWIERKVDIKTIQQSNLLHGKMYHIATPAGVQDAILGSSNFTVRGLGLGNSGN
                     NLELNLILDSNRDRQELKQWFDELWSSPTLVKDVKQEVLSYLKQLYDNHAPEFIYYKT
                     LYHIFEKFLGDTGKTDVDLGKTSLFETGIWKALFEFQRDGVKGAINKILKHNGCIIAD
                     SVGLGKTYEALAIIKYFELKNERVLVLCPKKLRANWTIYRTPDRLNPFDDDRFRFDVL
                     SHTDLSRDKGYSGDINLATLNWGNFDLVVIDESHNFRNNTPGRRDEEGVLIRKSRYQR
                     LMDDIIKSGVRTKVLLLSATPVNNDLKDLRNQLYFLTENRDDAFAESIGVASLKETLA
                     TAQKSFTLWAKQHSSKRRTGELMEKLNAGFFTLLDELTIARSRKHIQKYYKAGIATLG
                     GFPERKKPVSLYPEIDLLGRFLSYDKLNDEIAGYKLTLFNPSKYVKNEFRPLYQTTGA
                     DPFSQADRETYLIGMMKVNFLKRLESSVKSFEITMANTIAKIEALEQKIRNFLAAPGQ
                     HPDADPLELDLDDPGHDDELEEALLVGGKFKYKLEHLELDHGKNWLKDLKRDKDQLRI
                     LLNAAQNVSPDSDAKLAELKRIIAAKVANPTLNKDGAPNRKVLVFTAFADTAIYLYES
                     LLEWATCELGINMALVCGDSTRTTFGESAFDHILTNFSPVSKNRAKIPGMPQQGELDL
                     LIATDCISEGQNLQDCDYLVNYDIHWNPVRIIQRFGRIDRIHSAGRAVQLVNFWPTED
                     LNKYINLKNRVEARMALVDIAATFEDNILANKEIEDIVSEDLRYRDKQLLRLKDEILD
                     IEDMGDNVSLTEFTLDDFRLELLKYIEANRDTLESAPLGLYTCVPPRPEHKIIAPGVI
                     FCFRQEGPREADSGAKPDAGASINPLQPYFLVYVLADGNVRYGFAHPKQILDIYRALC
                     SGKSQPCTALCNLFDQQTNHGRDMKDYDILLDKAVASLAATFRKRAASGLTSSRSFIL
                     PDEREQVHEKTDLELVTWLVIKKGNA"
     gene            complement(16090..16962)
                     /locus_tag="PKI64_01710"
     CDS             complement(16090..16962)
                     /locus_tag="PKI64_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD99754.1"
                     /translation="MSKLSIRFFNDRKVRAVWDNANAKWWFAAVDVVAILSESANPAN
                     YWRVLKSRLTAEKNETITKCNAFKLRAPDGKLRLTDCLDSDGVLALAKNFPGNKARHF
                     LDWFLYSDNTTDGQSKKKAYSLFESGILQSLEPGTIKCLQQIHAYIFGGLYDFAGQIR
                     TKNISKGGFTFANARHLPATLASIEAMPETTFDEILDKYVEMNVAHPFMEGNGRATRI
                     WFDMMLKRSLRKCVDWSKINKNAYHEAMRQSISDSTKIKALVQAALTNKISDRATYMK
                     GIDYSYYYEQDPTP"
     gene            17267..18184
                     /locus_tag="PKI64_01715"
     CDS             17267..18184
                     /locus_tag="PKI64_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD99755.1"
                     /translation="MRKTGKTLLVGMLIGLVGWATAFGADGNLRYSITVTKFPNEAGW
                     SGRWDVGDGFTTILTDALQQSGKFIVLGDSEMRQEAMAEQDLAATGRMAGGAKAPQMG
                     RMTPAQLLVRGSITHVQDETGGGSGGLSFRGVSLGGSKGRAEVNITIYLVDSETGQVK
                     ASQKVIGQAGRRGLSVGYHGGALGGLGGNLAGFQNDNVGKACEDAVVQCVEFLEAQLA
                     SVPWKGSIMLVSGDKLVINRGTREGVTVGQKFQVGSTEDLVDPDTGEVLDSEMTTVGT
                     TVVTETKEKIAYCTPLSGAAKGMSVVPVQ"
     gene            complement(18221..18814)
                     /locus_tag="PKI64_01720"
     CDS             complement(18221..18814)
                     /locus_tag="PKI64_01720"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOD99756.1"
                     /translation="MTLEVFIATYGYYAIALGACFEGETILVLGGLAAHQGYLSFPGV
                     LACAFAATLATDHLYFYLGRHNGLAFLTRRPRWQAKSARVMDLIRRYQTPLALFFRFL
                     YGLRAIVPFSLGLSGISRLRFSLFDLLGSVAWVSFYGALGFFFGRTGQALLQELPRKE
                     YILFAAVAFIGGLLWARYRWRLRRTIRAAANNKKTPV"
     gene            complement(18852..19481)
                     /gene="upp"
                     /locus_tag="PKI64_01725"
     CDS             complement(18852..19481)
                     /gene="upp"
                     /locus_tag="PKI64_01725"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032884835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOD99757.1"
                     /translation="MPADAIILKHPLIEHKLAILRNRETPNPLFRATLNELSYLVVYE
                     ITRDLALRPVEIHTPLAPCTVQQLAEQILLIPILRAGLGMVDGILHLLPTAKIGHIGV
                     YRNEETLQPVEYYCKLPPGAEAMRVFLLDPMLATGGSLAYAITRLKEQGIRQISVVSI
                     LAAPEGIAALQAAHPDVTLYCAALDERLNEQGYILPGLGDCGDRLFGTL"
     gene            19736..20221
                     /gene="accB"
                     /locus_tag="PKI64_01730"
     CDS             19736..20221
                     /gene="accB"
                     /locus_tag="PKI64_01730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003782454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOD99758.1"
                     /translation="MDLERIKTVVELMREHDLSEFAIEDKDFKLQLKRGSAAPTVVMS
                     APGMAPAMAPMMAPAAAPAAAPAAPAAAPAPAPAQDESLTPIPSPLVGTFYRASSPDA
                     DPFVQVGSRVNKDTVVCIIEAMKVMNEIKAETSGVIKRILVENATAVQYGEPMFLIEK
                     G"
     gene            20228..21589
                     /gene="accC"
                     /locus_tag="PKI64_01735"
     CDS             20228..21589
                     /gene="accC"
                     /locus_tag="PKI64_01735"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOD99759.1"
                     /translation="MFKRILIANRGEIAVRVIRACREMGIRTVAVYSQADENCLHAQL
                     ADEAICIGKAPSSESYLKISNIISAAEVTDVDAIHPGFGFLSENAHFADICEECGITF
                     IGPSADNIRDMGDKSRAREMMKKAGVPVTPGSDGVVKSQDDALAIAKKLGYPVLIKAV
                     AGGGGKGMRIARNDMSLVQGYMTAQAEAERAFGNPDVYIEKLVERARHIEVQLLADNH
                     GHVYQLGERDCSIQRRHQKLLEETPSPALSPDRRKKLLKAAVRAAQACKLRNVATIEF
                     LWNEDAQEFYFMEMNTRIQVEHPITEEVTGADLVQLQIRAAAGEVLKFPDSEFEPRGH
                     AIEVRVNAENPYKNFTPSPGPVHAVHFPGGPGIRVDSHVYSGYVIPPYYDSMIGKIIA
                     RGKNREEALTRMVRALAEFKMEGPATTVPVAQALLADARFLRGEYNTHFLEQFMNDVF
                     WVS"
     gene            21611..23749
                     /locus_tag="PKI64_01740"
     CDS             21611..23749
                     /locus_tag="PKI64_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOD99760.1"
                     /translation="MKPARGSAWPAARSSLEHQVEGVLSRLGQGAAEPAGPPARARVV
                     VLGGGTGLSTILGGNPALAEWADQPEVGLAREFADVHVGVCTTDDGGSTGELVRRLPM
                     IGIGDTRKVMLAMVSRAALAKRYGAVGPLAKIVRGVFLHRFGPRAPTMAELRDPVRVL
                     KPELRQTCPPALRAVLASLTAGMQPWLAARLRVPGHSLGNLLLTAAIFRGVRQWRAPT
                     QTEVARGLAAAATVMGVPPGRVHAATATPGTLIYEYANGVVATGQARAARARRGCAVQ
                     RVRIAFAGTPRANPRLLAALRRADLIVYAPGSLYSSMLPVLLTPGVVAAIRANRRALK
                     VLGANLWIQEGETDMSFREESRGFWVSELIEAYGRNIPGGIAGLFDVVLATNLDTVPG
                     SIIRNYALEDKHPIHLDRARVASLGVMPVEASLFAGDRWPREAMIHHDPARFAAAVRT
                     VYEGLRQRPRKASRAALPPVTARRAALSAAARMDALRAALAGKTIAAAALRQAVEDFF
                     WVNQDLQPAHLAYVAGVRVVPDARWLRSREWDNVLGYYEPATRFIMLHEQTLRSRDAL
                     AANFAVALGESLLGRYIARKWWAPPVPGAGMQTYEIELRPPALRETLLTPGQLKTYLR
                     LAEMQPRAGEPLRYFRPVPQGMGFLPCGILFGLTFAWMLDNSYVPALDFEMHMLQWPA
                     SRLLPYQVRRRSLHQELVGFFRTVVLRQPE"
     gene            23813..24373
                     /locus_tag="PKI64_01745"
     CDS             23813..24373
                     /locus_tag="PKI64_01745"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD99761.1"
                     /translation="MAEQPSSEKRIVAAGRHLNFVVRNGWEFVERSRISGIVVVVGTT
                     RHGCLVLVSQWREPVQAWVVELPAGLAGDVAGLEREELVAAAHRELLEETGFRAATME
                     PLVSGPPSPGISNEVVTFFRARGLVRVTPGGGEGDEKTRAHAVPLSGLSEWLETVQAQ
                     KGMLVDPKVYAAAYLVRREMDEWMYE"
     gene            24366..25565
                     /locus_tag="PKI64_01750"
     CDS             24366..25565
                     /locus_tag="PKI64_01750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOD99762.1"
                     /translation="MSEVPATDAAAPRKAEPAVVYAAPADKRISVGAPSLLALANGKL
                     LVAFDQTGPDVKGLSGKKGNDTKRGRWMQGRVMASTDGGTSWQLAATYPFRRASLFRE
                     GGDVYLLGEAAGGLCLMRSPDGGASWSAPMELTGDLDLWLTPTALLATNEAWLLPCLI
                     PVENKMALTIFRAPRGASLMHRKAWTQGPPSAPLSDLIAAAPGAGCGVPAGPGAVTWR
                     EPVLLAISDGRHPWHDEQAVHVVGATSSGRQHWAALFRVALPTLTIEPQPAPTGEPWV
                     WLPLPGGHDKFDVFYDAPSRAYWVAGARGVPGLPLGREVDQPQGLRRIGLWTSDNLAE
                     WDTVATLVAGGDGPAGVRCDPALAVAGNDLVWACRAGGEHSRNVRETTRILCGRLPNF
                     RARSAAP"
     gene            25639..28854
                     /locus_tag="PKI64_01755"
     CDS             25639..28854
                     /locus_tag="PKI64_01755"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99763.1"
                     /translation="MSNTKQLPTSERQEPPRLLPFRWLAAIGLGGAALVLYLATLSRG
                     AFPGLPAKSLVWQLQLDIRPLLLDSLWGYLVWALSYLPGGSVAFWVGVSSAVFGALCV
                     VLLSLLTMRLRYSLHDQHDPAEVRREAQASLLAGVVAGLFLMGSIPFWILANRSLPGA
                     FHLLLLLSATFLFSEYQRTGRVGFLYALGLLYGVGISEFATFWMFAPLTALLVVRAML
                     QRAEFSMRVLVRTGLCVLPGLLLYPLNAWWMFQAPGVSLRGFGSLWSVVWYTWRDQWH
                     LIANAPQTSGFLLVMCLTVIPWGILFLMRSKKPAWRHSAWQVFLRVLVLAVAVATLYD
                     AAVSPWHFFGMQYLMATPYVILAACAGYVAGEFWVMGQTREHRNAGIGQPLRRVMAGV
                     GLLLPVAAVAALVLNQPVADGRFGSRLERLADETLDALDGRDVLLSDGIMDDMLRLRA
                     WERGLPVHVVPLRNTDTQLYRQYLALTFNSERQQSLLQISFPAFMQEFIAADENLRRL
                     ASIDFTDPLREYGYLEPDRMLYRAEVMEQDIDLPALVERQKPFWAEMEEWAALNLADR
                     NPAAGFRQYLLRLTSKVANNIGCMQVERGDKTGALATFQQARRLFPDNISALLNLLTI
                     AQTEKLPEEAEYTALWDEFKARHMDSRVMWALSALYGYVYNAGYLVRHGMMWAVSGKP
                     RLAEAELRRAAGRQGVNAAVKAFLGRAYLQGQDLQRSTEYYREAIQENPQDARSLLML
                     AEIALREEHYDEAEEWLQKVEAAGVPADRLRFERTALTYLRGDTDRAMQQLLDLVNKE
                     KDNVRAWALLAMLTSDAPDPTLHQRAVKALKELQGTSTDVRLLLAELDINQQNWAAAR
                     DKLEQLTRTNPRNVRAWELLVEVDFRERKRDLADDHVRSLLTLSPQNYTGNLLLGSLQ
                     YARGQFSLAESSYRAALRVRRDPAALNDLAYLLLINKPAAWGEARALVEEALAAQPAN
                     KIVFLSTRSELNLREGRLDEAEDDLQQVLTAMPNQAAALLLAAELYAARAAELAAADD
                     AAGAKRQQAAALDLANSLKDRQNELSLEQQQHLYGLLDAKP"
     gene            28942..32526
                     /locus_tag="PKI64_01760"
     CDS             28942..32526
                     /locus_tag="PKI64_01760"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99764.1"
                     /translation="MRNKPESEFYFFRPDKRLLPGDGRDPRVPLQTDPVINETPTDVH
                     LAIVLSVIMFVMYLASMSWEPFPGPPTILLLRCLDPLQPPDTQNFLWSWIMRGLALLP
                     FSTVAIPGGVFSALCGALGVGILTRLMIRIGYVLRNEPGHFSFLREIHARRLSGVTAA
                     LFLGLSTPFWVAATRTLPDTFDLLLLLLVAWFIGEYRHRGRPRDCLKASLLLGVCLTE
                     YPSAWAFLPAILFFMWREMFRWRSLHKWWLHALIFLGLLVGLLLLPLNAFLLFKHLAV
                     MRAKITPVEAMLQVLKDQFLPLVYFRFSPGFLPVVALVIVPWLAIFVMSTRSPWFYEW
                     GQVSVRLIFMAGFFAVAFNAAFAPWSLTGMFFLIVAPYVLLAVSVGYIAGEFWIMGEP
                     HALLDTGFLPRLRRGAMSLIALLFPVVFTASAVVNWPVVDGRYSRDATRFFQSVFTHL
                     GERDILFSSGQLDDSLRLLALEQHLTTRIVSAPHTGSSVYLNRLANAFEEEELSGPLA
                     QGDFGTFLENLLMSEDGPSRIGIIDLPDVFREYGYLVPDTMLYRLEATPDRVDWAALA
                     ASQQPFWDKMIRMTRNPLPEKSILRAFENYLREMVSRVANNLAYVQLGRGDDQAALAS
                     LRAAHEIWDHNVSVLLNLLELARKLHLPEQEDWEKELDKLKPDLGDERWVLAIRYGYV
                     WNPRAWMRRGHVWALSGIPATEEAARRKPAVSDADEAGNDNRNRVLDQSYLLWGQDAV
                     DETFLRGRLAKNGRDTDALMALCRLALRRNDPEVAEAYIMEALDFGLPEAKTQFDRAM
                     LAFVQGDKTRAIQMLRAQTVSTPRDMRVWTALLLLTDETDPINVEALKTLKNQAYAPV
                     GQYLALASIHMSRQQWADAQEELDRAIALDPRNTQAWEMMVQLAQQRGNRALLQAGLK
                     CLQEQDPDHFLQYQNAGVEAYQKGNLALAEAEFRKGLMRRRDALLLNNLAHVIMERQG
                     NMKDALKLVDEALLRLPGQPQMLSTRAEIYINLGRYADALQDLQESLRKQGRTNNLLL
                     LLALAYEGLGDWTRAETMSKTLAGLQAQLTPAQRERLAQLQGRLDAHTAATAAKAGEY
                     QQELTRLEAALRAQPDDAALRTARATILLQCARYDDARLDLEAAVRSRGETPELLFLL
                     VQAWEGAGNPRRALAYVKRLASMAETLDDAQRDQLHELRLGLELELEP"
     gene            complement(32539..33216)
                     /locus_tag="PKI64_01765"
     CDS             complement(32539..33216)
                     /locus_tag="PKI64_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOD99765.1"
                     /translation="MAHSQAGAFIFDLDGVIVDSNGLHVASWQAVAERHGFACPHPEY
                     IGKCGLRTRAVIRELLRWPVSEEEADRLGLEKEELYREWIRRDGIRPIPGVLDFLAAA
                     RQLGIPCAVGSSAPRENVDLCLQALALTAWFPVTISGADVQRGKPAPDIFLQAAAGLG
                     VPPAQCVVFEDAPAGIAAAHAAGMKAIGILSSHTRDELRGADRLVPDFTSLQPADILA
                     LAAAARQ"
     gene            complement(33220..33666)
                     /locus_tag="PKI64_01770"
     CDS             complement(33220..33666)
                     /locus_tag="PKI64_01770"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOD99766.1"
                     /translation="MKKVSLSELSAGDRRLVQAAIAIRQKAYAPFSQYKCGAALRDIK
                     NRIHVGCNIETIDFTLTTHAEMDAINGMIKTGTFQLKALACAVQSSSGYALPCGLCRQ
                     KIREFATGPDARIIAINLTKQGKIRHISVTTIGEIYPLPFTPAGLT"
     gene            complement(33706..34635)
                     /locus_tag="PKI64_01775"
     CDS             complement(33706..34635)
                     /locus_tag="PKI64_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99767.1"
                     /translation="MKKRMIFCGLLLGLVTQTGWALPPVLPTVPGAAPARDVSQSQPA
                     MRDITAAEVERTGLLPADIELLDNPAYKWQHLQTEHFILHHDQKMFAAKVARLGEQFY
                     QAISADLPQMKDRLSPVRSHIFIFRDARSWKAVVANTPGLDPWAASFVRGPVMYLQEI
                     GTGTADKMGTLAHEMTHLVFNRFLPIRLPLWLNEGLAEYYGEFAYRAARGMGQGKRNA
                     FRPLARWTPLTELLHATSYPLNTDPKVDAVGQFYATSKYLVGFLQLKQPMAKWDEFFN
                     RLLDGDDPLTALLETYGWTDIAALEKVFAQFVR"
     gene            34678..>35345
                     /locus_tag="PKI64_01780"
     CDS             34678..>35345
                     /locus_tag="PKI64_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD99768.1"
                     /translation="MQGKTQKAPTPWAVSGSLVAFPADRGRQTRLLDGFYRPGPRRGA
                     PLLVFVHGMGSNFYRSALKKAFLQLAPENGFAVLSYNNRGAERGTEDEPFSACLADLD
                     AAVEFGRRHGHRRLFFVGHSTGCQKIVFWQSRRRARAVSGLVLLAPADDYAITRQALG
                     RQFDRKVAWARKMLAAGKGSALIQALYERFTAKRFLSIANPRAIEANIFRYAGPLTHF
                     RRVKV"
BASE COUNT         6531 a         9165 c        12133 g         7506 t
ORIGIN      
        1 ttggtgcttt gtgcttggtt ggggaaagga agatgaatct cgccaagacg ccaaggccgc
       61 gaaggggaga aggttgaagg gggaatagcc ggagacatca acgcaagcga cgcgagagac
      121 gcaaaagaac gcaagcggag aaaacggctg gaaggtctgc gaccattcca gtccgacaaa
      181 atcgaaccgc ggccggaagg tctgcgaccg ttccggccct acaattcaat ctgagatggt
      241 ttttgggggg cgagagagcc cagatgtcag cggtcaggtt ttggcggggg agggggcggg
      301 gtgttttttg cgcatgcggg cgagttggat gatcatcatg acctggttgg tggaccaata
      361 gagggagagg cctgagggga aggaatagaa catgaaggtg aagatgatgg gcatcatcat
      421 catcattttc tgttgttggg gatcgctggt gggggtcatt ttttgctgga gccacatggt
      481 gaggcccatg aacagcggca gaatgttgag gccaaagggc aggtagttgc gcaggaggtt
      541 ttcgggcgtg gagaggtcgg caatccaaag gaacgaggag aagcggagtt cgatggcgcc
      601 gcgcagcacg gtgaagaggg cgaagaagac ggggatctga atgagcatgg ggaggcagcc
      661 gcccatgggg ttgattttgt gttctttgta gagcgccatc atttcctgct gctggcgctg
      721 ggggtcgttt ttatatttct cgcggacggc ggtgatcagg ggctggattt cctgcatgtg
      781 gcgggtgctg accatggatt tgtggttgag cggccagaag atgacgcgga tgaggaaggt
      841 gagcagaatg atggcgaggc cgtagttgta gggccagatg tggtcgtgga accagaccat
      901 gattttgagg atgaagatgc caatgggccg ccagaagccg agttggagga tatcttcctg
      961 atgggggccg ttggctttga gggcggcata gactttgggg ccggcataaa aggtgtagtg
     1021 ctgggtgatg gtttcgccgg cggggagggt gtagtccggc atttcgaggt aggcggtgac
     1081 gcgctgaatg ggcacggagg tgcgcgggaa gaggccaagg aaggatgaat gcacgggcgc
     1141 gccgtgctgg acaaacaggc gtccgaaccc gctgacgccg tcggtggggg tgaggatttg
     1201 cgtgaagtat ttatttttga cggcgaccca atccacgggg gtgggttcgt ggttggggcc
     1261 ggggatgagg ggcatttggc gggggccgct ggcggggaac catttgtcga acttgccggc
     1321 ccaatatttt acggaatcgc tgccggtgag ggtgtccacg cccagcaggg gaatctgctg
     1381 gcggccttct tcgccgggta ggttttccat gaagccggct tgaatgccgt agccgtggag
     1441 ggcgacgggg gcggcggatt ggttgcggag ccggtcttcc agttggagct ggtagccggc
     1501 gccaagggtg atgatgcgtt cgagaacgag gccgccgggg agggttttgc gataggtgat
     1561 ggaatcggca gtggcggcgg tgacctcgaa tgaggcgagt tcgcgcaggc cggggtagcc
     1621 gcgcggggcg gcagcgggct ggtcggagaa atcgaacgtg acgggggtgg ggtcgttctt
     1681 ggcggtgggg tatttcagga tggcggcttg cacgatgccg gcgccgtggg aggagaaggt
     1741 cagctcaagt tggtcattgg cgaggacggt taagatttcc gggccggcgg gttcttcggg
     1801 ggcgggggct tcttgggtgg cgggaggggg gggcggcact tctttggnnn nnnnnnngtt
     1861 gtgtgggggg gggggggggg gggtgggggg ggggggcggg gggaggcggg gggggcggcg
     1921 agggtggtat cggccggtag ggcgggggcg gtggcgggtt ccacggccac gggcgcgggc
     1981 ttggctttgg gcgggaaaat tttggcgaca aatttccggt caatggtcgg gtaggccaaa
     2041 aacaggataa aaattaaaat aatgggcagc aggtcttttt tattcatgct tggtttctcg
     2101 tttcacatca gatgattttg tcggtacggg atcatagccg ccggggttcc atgggtggca
     2161 acgggcaatc cgcagcaggc ccaaaaaaac gccgtaccct actccatggg tgcggatggc
     2221 ttccagcata taatttgagc aggagggctc aaagcggcag cagttgccca gcagcaggcg
     2281 aaaggttttc tggtagagcc ggatcagggc gcagagcaac ttggccaacg gattcatggg
     2341 ggcggcagca ggttggcctt ggcggccaac gccagcagat cctccacgac agccggccaa
     2401 ggcgcggcga ggatagagcg gcgggccacg aggatgatgt cgtcggcgcc ggtgaggcgg
     2461 tggcggttca agcggaaggc ctcgcgcagg cggcgcttgg cgcgggcgcg ttcgacggcg
     2521 ttgcccacct tgcggctggc caccacgccc aggcggcacg aggcatcggg cgcggcgcgg
     2581 cggaagagga ccatgtggcg gccgtgccag cgctggtttt gcgcgtaggc ttcatcaaac
     2641 gcggcggtgc ggcgcaaacg ctgtttgcgg ccaagcgtgg gccggacgca aaacgcccgg
     2701 ccggcatccg gggggggagc ggtcgggcgg tcgcggccgg gttgcatggc atgaacgccc
     2761 cggcgcgggg cggaaaacgg cggttatgcc ctgtcgctga tggacaggga tttgcggcct
     2821 ttggcgcggc ggcggctgag caccttgcgg ccattggcgg tggcttggcg ggcccggaag
     2881 ccgtgcttgc ggttgcgctt gatcttggac ggtttatatg tcggttgcat aaacggagcc
     2941 tttctcgaaa ctaaaaatga cgggcgtgtt tagcctgttt ggcggccggg tgtcaaccct
     3001 ttgttgcggg gcttggttgc catttgggtg cgcggtccca attgcgagca aagaaatcag
     3061 tcaggtgatt tgatgttttg aatccccttt acgcctcctt attccatcgg cctcataaat
     3121 gatgaccttt ggtggtgtgt tatcgggttt gtcgttgtcg ttcctgatcg ggtctcgcag
     3181 tgatcattta tcatcacggc tagcctgtat atcatgttcc aactccgcca agctcgaaag
     3241 ccgctcttcc tcccgcaggc gctggcggaa ttgggcgagt tgcttggtgc tgatctcctc
     3301 ggcaagcgcg tggctgatgc ttccggcgtg ggtcagaagc tcgcggccgt tcagggtgag
     3361 aatggcatcg agccgggcga cccaatcgct catggtcatg gcaatctgtt gttgcgcttg
     3421 ggcttcggca aacgcgagat attgttcgac caaaagcccc aacgccttca tttcggtttc
     3481 ggtaagataa tttttggctg tgacagcgtc ctgctgggtg atccgccgct ggttcttctt
     3541 gtcgtaggac tgcatcccca taaacggcag aatggcgttg gcgcgtcgcg ccacaagctc
     3601 cgccgccgtc tgcttgctga tcgcccaaag cagcttgttc tggacaacct tgaaaaaccg
     3661 caacgtctct tccgccgatg gatcataatc aatgctggtg gtgtagatgt ccttgatttt
     3721 ctggtaaaac acccgttcgg agaggcggat ttcgcggata cgcgcctgca gctcgtcgaa
     3781 ataggtcata ttgcgcccgg ttttcatgcg ctcatcattg agagcaaatc ccttgatgat
     3841 gtattccttg agaacggcgg tcgcccaaat gcggaactgc gtggcgcgtt tggaattcac
     3901 acgatagccg accgcaatga tggcatcgag gttgtagtgt ttcaggcggc gcgagacctc
     3961 acgattgccc tctttctgaa cttgtaagaa atctttacaa gttgaatcct gagataattc
     4021 tttgtcggca tatatatttt gcaggtgcat cgtgatgttt tgtggcgcaa catcaaaaag
     4081 tgcagccatc gccgcctggg ttagccatac ggtttcacct tcaagccgga cattaacatg
     4141 caccgccccg ccgtcggctt tgaagagcag aaactggctt tgattgttat cgctcatgcg
     4201 cagattcatc ctttctggcg cttctttagc ctgaataatt cacgattgga caccaaacaa
     4261 ttccgctgcc acggacgttg cagggcggga atggttgagg aactaaacct tcctgccggg
     4321 aaaggctgga aggtctgcga ccgttccaga acaccttctc gctggtacgg atatcgcgga
     4381 tgcgttcgag tagttcctgc caatagccgc caccgccgag ctccttcaag cggtcgtcat
     4441 ccatcgtgaa tcccttgatg atatattccc ggagtcgcac ggtggcccac tggcgaaacc
     4501 gggtggcgac cttggaattg atgcggtagc cgagggatat aatcatgtcg aggttgtaat
     4561 aggacatggt ataggatttg ccatcggtgg cagttgttcg gaatttccga acaactgaat
     4621 cggcggatag ctccccgctg tcgaagatgt ttttgatatg ctcactgaca gtggatttcg
     4681 tgcactgaaa caactgggcg agttggaggt tggtcagcca cacggtctcg ccttccagac
     4741 ggacgctgat tttggccact gcaccatccg cctcgtaaat gatgaccttc ggtggggtgt
     4801 tcccaggttt gtcgtggtta ctcatcgcat ggcctcaaca tgatgactac cttcttcgga
     4861 aattgacaag ggcgtctaag tcagtttcga tggagataat gtcggcggca ggattggtgc
     4921 gcttggcggt gaggatgcgg tcaacgaggg tggcgatggg tttttgtttg gctgtgggga
     4981 cggcggggaa tggcgtggat gccagacgca ggatggcggc acattcaaag gcggtgagcg
     5041 ggaggacggc cacgcggggc tggcggatga gagggaagtt ggcgagagcg gggttggatt
     5101 tgatttgggc aagggaaacg gggtcggcga gggtttgaag cggggcgaag tcgcaggtca
     5161 gccagcgcgg atcggcggat gttggatcgg gataggcggc gcgagtgacg gtcaggatac
     5221 cggtgaaaca acgatgctcc tggctgtgga aaaagagagc gaggtcgccg acggccattc
     5281 gggcgagatt gtttcgggct tgcgggcagc ggatgccgcg cggcgtgaaa ctgcccgctt
     5341 tcacaatgtc gtcccacgag gtgcgaaagg gggcgaactt caccagccaa aatttctttt
     5401 tcccggcctg ttgcattttc aaatccttgt gctaaagttc atgcagatac aaccaccatg
     5461 tgtggaacag tgatcagggc acgtctacca aggcgtgccc catttttttg ggcctccgcc
     5521 cacttcgtat gatagttggt gaattcatct cctttgactt tgggtctcgt gcgaatccca
     5581 taggaattgc accaaagtaa tgctgctgcc tacggcaagt tgcgcgtggc gcttttccaa
     5641 gcctctgtaa ttcgcgcttt tgccgtgacc gctcccgtaa gagtttcgga tggtagccat
     5701 gtttgagaca atttctctca ggttgcccaa cagccgtctg atagcagcgg cttctttcga
     5761 tgtgtcaggg atatcttggg gcgtcagaca taaaagttta acggtttcgc cgacgagttt
     5821 ccccatgtcc caaattttgt ctatggatac agaatgtgct tcaagaatcg ttcggcaaca
     5881 gctctccgca aactcttttg ctttgccgat tgcttcggtt ggattttgtg tcgtcatttc
     5941 cagcatcagt tttatctggg cagacatgta atcgcttgag aacttcttgg cgacagtatg
     6001 agcggaacca gccagcggga ccgcaacatt ggaaaaccgg tcaatgatag cgcgacatcg
     6061 cgcatagagt ttggcgatgt cctcatctcg ctcacagcgg gaggtacaat gaatttcaaa
     6121 gtgagccaac aggtccgtga atagcttgac gactttgctt gcctcagctt cattggcata
     6181 cgcggtcagc gacttgcctt ttgaaagtcc gtaatgatgg cataatgcga caccaacgct
     6241 atccagcgta aaattgtcga agtcgttggt tgagaaatcc agaacatacc caccgtcgtt
     6301 aaacagccgg aggaacgcac ctttttcaat gttcgtgagt tggggcatgg ttatctctta
     6361 ttaaaatcga atcgagatag ttcagtgatg atgtcgaatg cgtattgaac ttcggtgtcc
     6421 gtgaaagacg gagaagcgga atggcagagt ttattcagaa tgttggagcg gacttgttca
     6481 acgtcctgca tcagctgcgg tgtgataaag tctgactgtc cctcttgtac tcttgttctc
     6541 tgacgggatt ggataccttg ccataaattg tcagccgaaa tgttctttgg gtttttcttg
     6601 tatttgattt ctatcccaga gtcttggcac acccttgaaa gaatcgtctc aaatcgcgca
     6661 cggatataaa ctgcggcggc agtcaggtct ttgttgtcaa gatgaccttt ggcttttgcc
     6721 agtaggtcgc catcgcgcaa ctcgggacgg tcgaatgcca ttgtatcttt tgaaacaaga
     6781 aagagttctt ttgaaatcca cttttcgcca acccaagtcc tcgccaagtc ataccagacg
     6841 tggtcatggg tgaagaggaa aatttgccag ttggcaaagt cattctgcaa gagttccaag
     6901 aggggaatgc ggttggagag gtcgaggccg attaggacgt cgtcgaggac gaggatgccg
     6961 gggcggccgg cgcgagagaa tttggcgccg gcaaggtaga tggaaagtgc aatggcggac
     7021 aggcgggctt cattgaggaa gtgttgcggg gtgggaatag gcttcccgtt taattcaatt
     7081 aagagttcga tgctttcgcc tgtatatttt cggtctttct tttgtgtccc gtttgagtaa
     7141 gtgatcgttg gggtcgtaag gtttatgatc gtaccaggct ggagttttcc aagtattatg
     7201 ttggcttcga caaccacatt ggggataagt gttgataacg cggttgtcat cttttctgcg
     7261 gcggccactg catttctctg attcagggaa tttccgtaac ttcggactgc gtcaagcgca
     7321 gaggaaagaa tttcacgcga ttccctgatt gttcgtgaac caggaggcat cacgtctttc
     7381 aggattgtgc caacaagcag tgtgtagaga tttggattcg cattattttt atgcacgaag
     7441 ttggtttcga gcagggagcg ataatccaag aaaaaacagg aacgtgtgaa agcgttgaag
     7501 gcggttttgt ctttgtctat tgacgggtgg acagtgccga caccccatga aaatgaaggc
     7561 ggggatccgg acgtgagttc caccgtgatg ttatcgtctt tgccaatatc gaagatgttt
     7621 tgataggaag tgtatgcggt gtctttcaga tcgcggttga ggaggtcgcg gagggcctta
     7681 aagagggagg tcttgccgct gccgttttcg ccgtagagga ggaggttttt gccgtcgtcg
     7741 cccagggaaa cttcaaaatt gtgggggcgg tattgcggga aggcgcgata gccgttgatg
     7801 gagattttct tgatgcggag tggttgcggg gcgggcgttg ttgacgtgga ttccgatgtt
     7861 gcaactggac tagggacgag cggggtttcc tgtgtgtggg gcagggtaaa tggtgcttcg
     7921 gtggtgacgg cggaagaaat cgttgtggcg gtatcgttca ttttttatct acggcttcga
     7981 tggtttggat ttcttcgagg ttttggaggt cgttcagggc ggcgcggagg gggtgcttat
     8041 agtcggagat gagggtatgg agggcggaga gcgtggcgtt tgacatgtcg ccattaacgg
     8101 cgccgctgca accggacagg tgcggggcgg aaagggtgtg gaggtggagg ttgcgggcgt
     8161 ggagttcggc ggggaagaag agttcgtaga tgaggccgtt cagccattgc tcgaagagag
     8221 caatgacggc aagacggttg gggaagtctg cgttggcggg tttgtgaagc gcaatgatgg
     8281 ccgtggcaag cgtttcgcag tgttgttgtt ggtcgggtgt ggcggatggg ataggaatgc
     8341 ccccttgtgt ttcaacagac gcttgtataa agccgccttg caaatcgggg ctggtcatcc
     8401 gtaagtaata ccataccact ggagagttaa gaacagacac aatatactta tttgcctctg
     8461 caataaaatg gagtgcatta ttgatatagg catgtgcatc atcgaacgca tatgtgggtt
     8521 tgtccataaa acgccccata atgattttgt gcgtggaaaa ttctgtccaa taatcagccc
     8581 cccagcgttg gagagcatac cactcataac ggacacccgt ttccgccttg tttcgtgcgg
     8641 agagttcttt tttaaatttt gacagatgac gataaactgc aggatatttc gacttaaact
     8701 cattttccgc ttcttttgag gcgcctcgaa ttgatctatt caaatgtaac gggaaatgcc
     8761 atgggatata aagtaaccaa agccctgttt catcataccg ccaacgtttg acatctcgcc
     8821 cgcgaagata cggcttcaca acgtcttttg aggatggatg ctcggctatc agggcattct
     8881 tagttgcttc atccacaaca aaggcctcgt tgaaccctgt ttttattccg taataaattc
     8941 tcccgttgca gtattctttg agtggagttc ccgctttttt gatgcgctca aaaaggacaa
     9001 tgtcgtattc gtcttcgagg cgccagccgg agggattgag gtttcgttgc cgcaacgtgt
     9061 tgtgctgctc gtcgaagact tgagggaagg tgcttttttc agggccaagt cgccatgtga
     9121 aaacggggaa tggacgttcg gggtttttga aatgccgttt agtgaaggac tcctcgcttt
     9181 ctcctttgcg gagtttgggc agttgcttcc gctcaacatc ctttgttttc ttggtaacaa
     9241 agatggcagg ataggcgatg gcggtaaaga tgttggtgtc gccaaagtcg aggatggcgg
     9301 cgggttcggt ggcatgggcg aggtagatgc ggagtttttc gccgtaggcg gagcggaagt
     9361 atttattgga ggtgatgaaa gagaggaagc cgccaatttt gagcaattgc agggagcgtt
     9421 caaagaaata gacgaagagg tcggcggtgc cggcgtaggt ttcgtattgg tcttcgagga
     9481 tggttttaag gggttgggcg ttgccttcgg tgtcgagggc cgtcatgggc ttgagttctt
     9541 cctgacgaac gtagggcggg ttgccgatga gcaggtcgaa tttttcggag aggccgaaca
     9601 tccattcggg gtcgaagaag tcggcggcgg tgttggtgtt gtaagggttc caacgggcga
     9661 tgcggtcggc gtcggaggtg gacatggtgc cggtggcggc gagttcggcg gcgagttgtt
     9721 tgcagagttc gcggtcttgt ttgcggaggg cttttttgcg cgggtaggag cgggcggtga
     9781 agtagtcgtg gcggatggtt ttgagttgtg cttcgagttt gcggacttgt tcggaaccga
     9841 ggaggagttg gccgcgctgg aggccgatga tggtgttggc ggcgacgatt ttggtttcga
     9901 ggttggggag ggggaggatg ccgtaattgg ggagcgcggg gtcaatgggt tggtcaacga
     9961 tgagggtgat gaagaagcgg agttttgcga tttggacggc gaggggttgg atgtccacgc
    10021 cgtagagggt gttttcgatg aggtagagtt tgcgtccgta gtcgccgttg tcgtgtgcga
    10081 aggcgcgttc gatggcggcg agtgcggctt cgcgggcggg ggtatcggcg atttggctgg
    10141 cggtgtcgag ttggcgttgt ttccagaggg cgttttcggg gtcgagtttc gcgaggatgt
    10201 gggtgagttt gtgaagcgcg cccatcggga aggcgccgga gccgcaggcg gggtcgagga
    10261 ttttgaggtt gtctatggcg ttgatgaggg cggcggtttc ctgtttggag aagtcggggc
    10321 gggcttcggg gtcgggagag aagagggcgg tgagttttgc ctcgtgtttg gtgtcttttg
    10381 attttgggag gacggcggag agggtggggc gaagggcttg ctcgaccatc catgtgacga
    10441 cgtaatcggg ggtgtagaag gagccggtgg cgttgcgggc gacggtaccg gtttcggggt
    10501 tgtaggaggc gaggaggttt tcaaagatgt tgccgaggag gtcggggtcg agggcaagtt
    10561 cctcttcaag aggggtattt tcggcaacgg tgaagacgta gtggttgagg atggtaagga
    10621 ggggggcgac gggaacggcg gtttgggagg aattgccggt ggcggcggag aggtcggcgg
    10681 tttggttggg gccgaagaaa aggaagttgg gaatacgggg gcatttggcg gggtcagagg
    10741 aaaagccgtc ggcgcgaatc tcgcgggtga aattggagtt gccttgttgg acgcgctcgt
    10801 cgaggcattc aaagagaccg ccgttgagga aagggattgt gcgaagaagg gcagctaggg
    10861 catcggggga gcgaagctgc tcggcgtgac gccagagggt gtgaaccatg tggtcatctt
    10921 tgctgttccg cgcaaaacga cggttggaag gggattcagg ggtatccatt tccgtattaa
    10981 gggtggcaaa aaagaggttt tggaggatag cggtgtagta ggtggtggcg gttggggaag
    11041 cgtcgtgaag gaggtggcgg atgtgttttt cagagaagag ctcttcgtgg acgaggggac
    11101 ggccgttggg gaggcgacgc tcttttagga accagcaaaa gagaatgcgc gtgaggaggc
    11161 ggatgagaaa gagagaaggt ttcttggtgt cggcgtcgag cggggcatca ggtggcggag
    11221 tgatgttttc gcgcgcccag aagaaccagt tggcaatggc gtagtaggcg gttttacgga
    11281 gcagttcgac attgaacagc tcctcccagg cggcgtggag ggcggtgaag gagcggatgg
    11341 gggtgtggcc gctttgcatg ggactggaca gggatgggac ggcgagggag gcgaggatgt
    11401 cgaggtgccc gcgatggggg gtcagtgtgt tgatgtcgcg gatgagggtg actttgccga
    11461 ggacgtcttt ctcgctatcg cgaaggttgg cgcggcggtt gatgacggca agggtgatgg
    11521 ctgcaccggt cttgaacagc accatgacgg gctgggggaa gaggcggttg agctggcggg
    11581 tgatggtggc gagttgggtg cgggtgtagg aggtaccgga aagttcgatg gcaaaaaaga
    11641 tgtaggagtc gtggcgggtg cgttcgaagc cttctttgaa gagtgagttt tcgcctgaga
    11701 gttcggtgtc ggtgagttgg aagaggaggt gcgcggaacg ccagtgggag acaagagcgg
    11761 cggcggggtt gaaggtggcg tgaacgcccc ccgcagagct ggatgcaaaa gtggaaagga
    11821 aggcatcggg ggagccgccg gtgtcgaggg tgcgcgggga tgcgtagccg aggacggcga
    11881 aaaagtcgag ggcagcggca cgaaaatcgg ttgccgtggc ggcctcggcg aggggctgat
    11941 tggcgaaggc tttgaggctc tctttgatta tggtaaggag tgtcataatt gggtacctta
    12001 gcaggtttgg cagggggttg gaacggacaa aacggggata gaagaaacgg acataacgga
    12061 cagaacggag gggagggaac ggacaaaacg gactaaaagg aagggggagt gtttttggta
    12121 cgggcagtgg tacgggcttg gtggaggcgt tcggagaagc cgccgccttt ttcaaagtcc
    12181 tgtgcctggc ggttcatcaa gcgctcggca agccaatgtg cctgagcaga gagtatggca
    12241 ccaagattgg cggcacgttc ttccggtgtg cctgcagcgc ccttacggtt gacccaggct
    12301 gccgcctctg acaggctttt ggggcgcagt tggcgcgcct cttcaaattc cgcttcgccc
    12361 tgctgccagt ggcgcagatt gcggcgttcg aggcatttga cataatcttt gtgcagttct
    12421 cccaggctgg aacgggcaac attggtcagg ttcatctcca gttttttgga ggtagcgcta
    12481 tcttcactgc cttcagctat attctggtag ctactgcggg ctgcctgtac catctggtca
    12541 tggtgtcggt cagagaatgg tatataccgc tcacaaaagc ggcaggtgaa ggcatagagc
    12601 agctctgcta tctggtatgc cttcaggttg cggtagccgc tgtgctgacc gtaaatccgg
    12661 ccggaggcat cagagtccaa tgcccgtccg tcttgccggt ctgcaaagtc agcatttcca
    12721 ttctgtctgt tttgtccgtt atccctgttc atgcatttcc cttcttgatg accagccagg
    12781 tgacgagttc gaggtcggtc ttttcgtgga cttgctcccg ctcgtcgggc aggatgaagc
    12841 tgcgggagga ggtgaggccg gaggcggcgc gtttgcggaa ggtggcggcg agggaggcca
    12901 cggctttgtc gagcaggatg tcgtagtcct tcatgtcgcg gccgtgattg gtctgctggt
    12961 cgaagaggtt gcagagagcg gtgcaaggct ggcttttgcc ggaacagagg gcgcggtaga
    13021 tgtcgagaat ctgcttgggg tgcgcgaagc cgtagcggac attgccgtcg gcgaggacat
    13081 agaccaggaa atagggttgg agcggattga tggacgcgcc ggcatcgggc ttggcgccgg
    13141 agtccgcttc gcgcggcccc tcctggcgga aacagaagat gacgccgggg gcgatgatct
    13201 tgtgttcggg acgcggcggc acgcaggtgt agaggccgag gggcgcggat tcgagggtgt
    13261 cgcggttggc ctcgatgtat ttcaggagtt cgagccggaa gtcgtcgagg gtgaactcgg
    13321 tgagggagac gttgtcgccc atgtcttcga tgtcgaggat ttcgtctttg aggcggagaa
    13381 gctgcttgtc gcggtagcgg aggtcttcgg agacgatgtc ctcgatctcc ttgttcgcca
    13441 ggatgttgtc ctcgaaggtg gcggctatgt ccacgagagc catgcgggct tcgacgcggt
    13501 ttttgaggtt gatgtatttg ttgaggtctt cggtgggcca gaagttgacg agctgcacgg
    13561 cgcgaccggc gctgtggatg cggtcaatgc ggccgaaacg ctggataatg cgcaccgggt
    13621 tccagtggat gtcgtaattg acgaggtagt cgcagtcttg caggttctgg ccttcgctga
    13681 tgcagtcggt ggcgatcagc aggtcgagct cgccctgctg cggcatgccg ggtattttgg
    13741 cgcggttctt ggacacgggg gagaagttgg tgaggatgtg gtcgaaggcg ctctcgccga
    13801 aggtggtgcg cgtgctgtcg ccgcagacca gggccatgtt gatacccagc tcgcacgtgg
    13861 cccattcgag aagcgactcg tagagataga tggcggtatc ggcgaacgcc gtgaagacga
    13921 gcacctttcg gttcggcgcg ccgtccttgt tgagggtggg gtttgcgacc ttggcggcga
    13981 tgatccgctt gagttcggcg agcttggcgt ccgagtccgg cgagacgttt tgcgcggcgt
    14041 tcaagaggat gcggagctgg tccttgtccc tcttgaggtc tttgagccag ttcttgccgt
    14101 gatcgagctc cagatgctcc agcttgtatt tgaacttgcc gccgacgagc agggcctctt
    14161 cgagttcgtc atcgtggccg ggatcgtcca ggtcgagttc gagcgggtcg gcatcgggat
    14221 gctggccggg tgctgccagg aaatttctga ttttctgttc gagcgcctcg attttggcga
    14281 tggtgttcgc catcgtgatc tcaaaggatt tcacggagct ttcgaggcgt ttgaggaagt
    14341 tgaccttcat catgccgatg aggtaggtct cgcggtcggc ttgggagaaa ggatcggcgc
    14401 ccgtggtctg gtaaaggggc ctgaactcgt tcttgacgta tttcgacggg ttgaacaggg
    14461 tcagcttgta gcctgcgatc tcgtcgttga gcttgtcgta ggagaggaag cgaccgagca
    14521 ggtctatctc gggataaagg gagaccggct ttttccgttc ggggaacccg ccgagggtgg
    14581 caatgccggc cttgtaatat ttctggatgt gtttgcgcga gcgggcgatg gtcagttcgt
    14641 cgagcagggt gaagaagccg gcgttgagct tctccatcag ctcgcccgtc ctgcgcttcg
    14701 agctgtgctg cttggcccag agggtgaagc ttttctgggc ggtggcgagc gtttccttga
    14761 ggctggcgac gccgatggac tcggcaaagg catcgtcacg attttcggtc aggaaataga
    14821 gctggttgcg gaggtctttg agatcgttgt tgaccggcgt ggcggacagc aggagcactt
    14881 tggtgcggac gcctgatttg atgatgtcat ccatgaggcg ctgatagcgg cttttgcgga
    14941 tgaggacgcc ctcttcgtcg cgtcgtccgg gggtgttgtt tcggaaattg tgggattcgt
    15001 caatgacgac gaggtcgaag ttgccccagt tgagggttgc aagattgatg tcgcccgagt
    15061 agcccttgtc gcggctgagg tctgtgtgag agagcacgtc gaaccggaac cggtcatcgt
    15121 cgaacgggtt gagccggtcc ggggtgcggt agatggtcca gtttgcgcgg agttttttcg
    15181 ggcaaagcac caggacgcgc tcattcttca gttcaaagta cttgattatc gcgagggcct
    15241 cataggtttt gcccaagccg acgctgtccg cgatgatgca gccattgtgt ttcagaatct
    15301 tgttgatggc tcctttgacg ccgtcgcgct ggaactcgaa aagcgccttc cagatgcccg
    15361 tctcgaagag gctggtcttg cccaagtcca catccgtctt gccggtatcg ccaaggaact
    15421 tctcgaagat gtgatagagc gtcttgtaat agatgaactc gggggcgtga ttgtcgtaaa
    15481 gctgcttgag atagctgagg acttcctgtt tgacatcctt gacgagtgtc ggacttgacc
    15541 acagttcatc gaaccactgc ttgagttcct gccggtcgcg gttgctatcg aggatgagat
    15601 tcagctcgag gttgtttccg ctgttgccca ggccgaggcc gcgcacggtg aagttggaac
    15661 tgccgaggat tgcatcctgc acacccgccg gggtggcgat gtggtacatt tttccgtgca
    15721 agaggttgga ctgttggatg gtcttgatgt ccaccttgcg ctcgatccag tcggcacact
    15781 ccttggcgac acgcttctgc tggagtttgt tggccagttt caggccggcg gcatcaagga
    15841 tgaaagcctt cttctcggcc ttgctggggt cgaggcggtt gacaaaggtg ggttcgccga
    15901 acaggaagtc gaggtggccg atggagtcga gaacctcttt caaggcatcg taggcgtaga
    15961 tggtgaaata ggccgagacg aacgagaggc gcgagtcggc cgtgattttt tccttcaaaa
    16021 aatcggcgac gctgccgcgg gcgtggttgt cgcggagggc ggatgagggg gggctatcgg
    16081 agggtttctt catggggtgg ggtcctgttc gtagtagtag gagtagtcaa tgcccttcat
    16141 ataggttgcg cggtcgctga tcttgttcgt cagggcggct tggaccaagg ctttgatttt
    16201 ggtgctatcg ctgatgctct ggcgcatggc ttcatgatag gcgtttttat tgattttgct
    16261 ccaatccacg catttcctga gcgagcgctt gagcatcatg tcgaaccaga tgcgggtagc
    16321 gcggccgttg ccctccatga aggggtgtgc gacgttcatc tcgacgtatt tgtcgaggat
    16381 ttcatcgaag gtcgtttccg gcattgcttc gatactggcg agagtggcgg ggaggtgacg
    16441 ggcattggcg aaggtgaagc cgcctttgct gatgtttttg gtgcgaattt ggccggcgaa
    16501 atcgtaaagc ccaccgaaga tataagcgtg aatctgttgc aggcatttga tggtgccggg
    16561 ttcgagactt tgcaagatgc cgctctcgaa taacgaatag gccttctttt tgctttggcc
    16621 gtcggtggtg ttgtcactat agagaaacca gtcgaggaaa tgccttgcct tgttgccggg
    16681 gaaattcttg gcgagcgcga ggacgccgtc gctgtcgagg cagtcggtga ggcggagctt
    16741 gccgtcgggg gccctcaatt tgaaagcgtt acattttgta atggtttcat tcttttcagc
    16801 agtcagacgg ctcttgagga cgcgccagta attggcaggg tttgcgcttt cgctaaggat
    16861 ggccacgaca tccacggccg cgaaccacca tttggcgttg gcgttgtccc agacggcgcg
    16921 gactttgcgg tcgttgaaga agcggatgga gagtttgctc acggtacggt ttccaattta
    16981 tagccgacga gcattgcagt tttcggtttt gtaattttgt taagcattgc aatcaattgt
    17041 cccggatttg accgtgaaaa tcaacgccaa gcgggcgatt ttgaccggaa cctccaatgg
    17101 gcgccccctg ttcctgtttc acgaacaatt gattttcaag ggctgaccca gcccccgcca
    17161 tggggccatg gacgtttgcg gtaccccccc ttgtttttcg tggtgcggca gactatattg
    17221 cccggcaaaa aacatgaggc ctcgtgccgt acaacaaggg aagaaaatga gaaaaacggg
    17281 aaagacgttg ctggtgggga tgttaattgg gctcgtgggt tgggcgacgg cgtttggcgc
    17341 ggatggcaat ttgcgctatt cgatcacggt caccaaattc ccgaacgaag ccggctggtc
    17401 cgggcgctgg gatgtgggcg acgggttcac gacgatcctg actgatgcgt tgcagcagag
    17461 cgggaaattc attgttttgg gcgatagcga aatgcggcag gaggcgatgg cggagcagga
    17521 tttggcggcc accggccgga tggcgggcgg cgcgaaggct ccgcaaatgg gccgcatgac
    17581 cccggcgcag ttgctggtgc gcggctccat tacgcacgtg caggatgaaa ccggcggcgg
    17641 cagcggcggg cttagttttc gtggggtttc gctgggcggg tccaaggggc gcgcggaagt
    17701 gaacatcacg atttatctgg tggacagcga gaccggccaa gtcaaggcgt cgcagaaggt
    17761 gattgggcag gcgggtcggc gtgggctgag cgtgggttat cacggcggcg cgttgggcgg
    17821 cttgggcggc aatttggccg gtttccagaa cgacaacgtg ggcaaggcgt gcgaagatgc
    17881 cgtggtgcag tgcgtggaat ttctggaggc ccagttggcc agcgtgccgt ggaaaggctc
    17941 cattatgctc gtcagcggcg acaagctggt gatcaaccgc gggacgcgcg aaggcgtgac
    18001 ggttggccag aagttccaag tgggcagcac cgaagacttg gtggatccgg acaccggcga
    18061 agtgctggat tcggaaatga cgaccgtggg caccacggtg gtgactgaaa ccaaggagaa
    18121 gattgcctat tgcacaccgc tcagtggtgc ggcgaaaggc atgagcgtgg tgccggtaca
    18181 atagcgcacg gccaaacgtg acgaagcgcc ggtctgcggg tcagaccggc gtttttttgt
    18241 tgttggcggc ggcgcggatg gtgcggcgca gtcgccagcg gtagcgggcc cagagcaggc
    18301 cgccgatgaa ggccacggcg gcaaagagaa tgtattcctt gcgcggtagt tcttgcagca
    18361 gggcttggcc ggtgcggcca aagaaaaagc ccagcgcgcc ataaaacgac acccaggcga
    18421 ccgagcccag cagatcgaag agtgagaagc gcaggcgtga aatgccgctg aggcccagcg
    18481 aaaaggggac gatggcgcgc agcccgtaca aaaagcggaa gaacaacgcc agcggggttt
    18541 ggtagcggcg gatgaggtcc atcacgcgcg cggatttggc ctgccagcgc gggcggcggg
    18601 tcagaaaggc cagaccattg tggcggccga ggtaaaagta gagatgatcc gtggcgaggg
    18661 tggcggcgaa cgcgcaagcc agcacgccgg ggaaagagag atagccctga tgcgcggcga
    18721 ggccgcccag caccaagatg gtttcgccct cgaagcaggc gccgagcgca atcgcatagt
    18781 agccgtaggt ggcgatgaaa acttccagtg tcatggccgg gggcgggcgg ggcggtgagg
    18841 cgcgggtgtg gtcacagggt tccgaagagg cggtcgccgc aatcgccgag acccggcaga
    18901 atgtagcctt gttcgttcag ccgttcatcg agcgcggcgc aatagagcgt gacatcggga
    18961 tgggcagctt gcagcgcggc gatgccctcg ggcgcggcga ggatggatac cacgctgatt
    19021 tgccggatgc cctgttcctt gagccgcgtg atggcgtagg ccagcgagcc gcctgtggcc
    19081 agcatgggat ccagcaggaa cacgcgcatc gcttcagcgc ccggcggcag tttgcagtag
    19141 tactccactg gttgaagggt ttcttcgttg cggtagaccc cgatgtggcc gattttggcg
    19201 gtggggagca gatgcaaaat gccatccacc atgccgagcc cggcgcgtag aatggggatc
    19261 aacaggattt gctccgccag ttgttggacg gtgcagggcg cgaggggggt gtggatttcc
    19321 actgggcgca gggcgaggtc gcgcgtgatt tcatagacca caagatagga cagttcgttg
    19381 agcgtggcac ggaaaagtgg attgggtgtt tcgcggttgc gcaggatggc cagtttgtgc
    19441 tcgatgagcg ggtgcttcag gataatggcg tcggctggca tggggcgctc cttgcttcaa
    19501 aagctcaaaa acctgtttag cagcgcttcc accaaatgga aagcgggaaa agcctgccgg
    19561 cgatggcggg ttgaagaagc ggggaggggg agcggcaaaa aaatccggat gaaaaaggtt
    19621 gcttcccacc cgaaaacaag tagatatgaa aaaggttgcc acggggctgg taaaatgttt
    19681 tacttgcccc acaaggttgg tggagagccg gccatatcga aggagttcag aacctatgga
    19741 cttggaacgg atcaagacag ttgtcgagct gatgcgtgag cacgacttga gcgaatttgc
    19801 gattgaagac aaagatttca agttgcagtt gaagcggggc agcgccgccc cgaccgtggt
    19861 gatgtcggcc cccgggatgg cgccggctat ggccccaatg atggcacccg ccgccgcccc
    19921 agcggctgct ccggcagcgc ccgctgcggc accggccccc gcaccggcgc aggatgagag
    19981 cctcacgccg attccctcgc cgctggtggg cacgttctat cgggccagct cgccggatgc
    20041 ggatccgttt gtgcaagtgg gcagccgcgt caacaaggac acagtggtgt gcatcatcga
    20101 agcgatgaag gtcatgaacg agatcaaggc cgagaccagc ggcgtgatca agcgtatttt
    20161 ggtggaaaac gccacggcgg tgcaatacgg cgagccgatg ttcctcatcg agaagggctg
    20221 aggccgcatg ttcaagcgaa tcctgatcgc gaaccggggc gagattgccg tccgcgtgat
    20281 ccgggcctgc cgggaaatgg gcatccggac ggtggccgtc tattcgcagg cggacgaaaa
    20341 ctgcctgcat gcgcaactgg ccgatgaagc gatctgcatc ggcaaagcgc cgtcctcgga
    20401 aagctacctg aagatttcca acatcatcag cgcggcggaa gtcacggatg tggatgccat
    20461 tcatccgggc tttggctttt tgtcggaaaa cgcgcatttt gcggacatct gcgaggagtg
    20521 cggcattacg ttcattggac cgtcggcgga caacatccgc gacatgggcg acaaatcgcg
    20581 tgcgcgggag atgatgaaga aagccggcgt gccggtgact cccggtagtg acggcgtggt
    20641 gaagagccag gatgacgcgc tggccatagc caaaaaactg ggctatccag tgctgatcaa
    20701 ggccgtggcc ggcggcggcg gcaaggggat gcggattgcc cgcaacgaca tgagcctggt
    20761 gcagggctac atgaccgcgc aggccgaggc cgagcgcgca tttggcaatc cggatgttta
    20821 cattgaaaaa ctggttgagc gggcacggca catcgaagtg caactgctgg cggacaacca
    20881 cggccatgtg tatcagcttg gtgagcgcga ttgctcgatc cagcggcggc accaaaagtt
    20941 gctggaggaa acgcccagcc cggcgctatc gccggaccgc cgcaaaaagc tgctgaaagc
    21001 ggcggtgcgc gcggcgcaag cctgcaagct gcgcaacgtg gccacgatcg agttcctgtg
    21061 gaacgaggac gcgcaggagt tctacttcat ggaaatgaac acgcgcattc aggtggagca
    21121 cccgatcacc gaagaggtga cgggcgccga tctggtacag ttgcaaatcc gcgccgccgc
    21181 cggcgaagtc ctcaagttcc ccgacagcga gtttgagccg cgcggccatg ccatcgaagt
    21241 gcgcgtgaac gcggagaacc catataaaaa cttcacgccc tcgccgggcc cggtgcacgc
    21301 tgtgcatttt ccgggcggcc ccggcatccg ggtggattcg catgtgtatt ccggctacgt
    21361 catcccgccg tattatgaca gcatgatcgg caagatcatc gcgcgcggca aaaaccgcga
    21421 agaagcgctg acccgcatgg tgcgggcgct ggcggagttc aaaatggaag gcccagccac
    21481 cacggtgccg gtggcgcagg cattgctggc ggatgcccgt ttcctgcgcg gggaatacaa
    21541 cacccacttc cttgaacaat tcatgaacga cgtcttctgg gtgtcctgat tgcctgacgg
    21601 agaaacccgg atgaaacctg cccgagggag cgcatggccg gcggcgcgta gctcgctgga
    21661 gcatcaggtg gaaggcgtat tgagccggtt ggggcagggg gcggcggaac cggccggccc
    21721 gccggcccgg gcgcgcgtgg tggtgctggg cggcggcacg ggactttcca cgattcttgg
    21781 cggcaacccg gcgctggcgg aatgggcaga ccaacccgaa gtgggattgg cgcgcgagtt
    21841 tgccgatgta cacgtggggg tttgcaccac ggatgatggc ggttccaccg gcgagctggt
    21901 gcgccggctg ccgatgatcg gcattggcga cacgcgcaag gtcatgctgg ccatggtgtc
    21961 gcgcgcggcg ctcgccaagc gctacggcgc ggtggggccg ttggccaaaa tcgtgcgcgg
    22021 cgtttttctg catcgttttg gcccgcgcgc gccgaccatg gcggagctgc gcgatcccgt
    22081 gcgcgtgctc aagccggagc tgcgccagac ctgcccgccg gcattgcgcg cggtattggc
    22141 gagcctgacc gccggaatgc aaccgtggtt ggcggcgcga ctgcgggtgc cggggcactc
    22201 gctgggcaat ctgctgttga ccgcggccat ctttcggggc gtgcgccagt ggcgcgcgcc
    22261 gacgcaaacc gaggtggccc gcgggctggc cgcggcagcg acagtgatgg gcgtgccccc
    22321 cgggcgcgtc cacgcggcca cggcgacgcc gggtaccttg atctatgaat atgccaacgg
    22381 ggtggtggcc acggggcagg cgcgggccgc gcgggcgcgg cgtggctgcg cggtgcagcg
    22441 ggtgcggatt gcatttgccg gaaccccgcg ggccaatcct cggctgctgg ctgctctgcg
    22501 ccgcgcggac ttgatcgtgt acgcgccggg cagcctctac agcagcatgt tgccagtgct
    22561 gttgaccccg ggcgtggtgg cggccatccg cgccaatcgg cgcgcgctaa aagtgctggg
    22621 cgccaacctc tggattcagg aaggcgaaac cgacatgtcc tttcgcgagg aaagccgcgg
    22681 cttctgggtg tcggaactga tcgaagccta cgggcgcaac attcccggcg gcattgccgg
    22741 tttgtttgat gtggtgttgg ccaccaatct tgacaccgtg cccggcagca tcattcgcaa
    22801 ctatgcgctg gaggacaagc acccgattca cctggaccgg gcgcgggtgg cttcgctcgg
    22861 cgtaatgcca gtggaagcca gtttgtttgc cggcgaccgc tggccacgcg aggccatgat
    22921 tcaccacgac cccgcgcgtt ttgccgccgc ggtgcgcacg gtgtatgaag ggctgcggca
    22981 gcggccgcgc aaagcgtccc gcgccgccct gccgcccgtg accgcgcggc gggccgcctt
    23041 gagcgccgcc gcgcggatgg atgcgttgcg cgccgcgctg gccggcaaaa ccatcgccgc
    23101 cgccgcgctg cggcaggccg tggaggattt tttctgggtg aaccaggatt tgcagccagc
    23161 gcatttggct tatgtggccg gtgtgcgcgt ggtgccggat gcgcgttggt tgcgcagccg
    23221 cgagtgggat aacgtgctgg gatactacga gccggcaacc cggttcatca tgctgcatga
    23281 gcaaacgctg cgttcgcggg acgcattggc ggctaacttc gccgttgcct tgggggaatc
    23341 gctgctgggg cgctacatcg cgcgcaagtg gtgggcgccg cccgtgcccg gtgcgggaat
    23401 gcaaacctac gagatcgaat tgcgcccgcc ggccttgcgc gaaaccctgc tcacgcccgg
    23461 ccaactgaaa acctatctgc ggctggcgga aatgcagccg cgggcagggg agcccctgcg
    23521 ctatttccgg ccggtgcccc aaggcatggg ctttctgccg tgcgggattt tgttcgggct
    23581 gacgttcgcg tggatgctgg acaactcgta tgtgccggcg ctggattttg aaatgcacat
    23641 gctgcagtgg ccggcctcgc gcctgctgcc gtatcaggtg cgccggcgga gcctgcacca
    23701 ggaactggtg ggcttcttcc gaacggttgt cttgcgccag cccgaataag cgagcgccac
    23761 tggcgacttt cttgcttttg ttgggcggcg cgggtaagta gtatgcgtcc gtatggctga
    23821 acaaccttct tctgaaaagc gtattgtggc cgccggccgg cacctgaact ttgtggtgcg
    23881 caatggctgg gagtttgtgg aacgttcgcg tatttctggg attgtggtgg tggtgggcac
    23941 cacgcgccac gggtgcttgg tgctggtgtc gcagtggcgc gagccggtgc aggcgtgggt
    24001 ggtggagttg cccgcggggc ttgcgggcga cgtcgccggc ctcgagcgcg aggaactcgt
    24061 ggccgccgcg caccgcgaat tattggagga aacaggcttc cgcgcggcca ccatggaacc
    24121 gctggtttcc ggcccgccca gcccgggcat ttccaacgaa gtggtgacgt tcttccgcgc
    24181 gcgcggtttg gtgcgggtga cgcccggcgg cggcgagggg gatgaaaaaa cacgcgccca
    24241 cgcggtgccg ctgtcgggtc tttccgagtg gctggagacc gtgcaggcgc aaaagggaat
    24301 gctggtggac cccaaggtgt atgccgcggc ctacctcgtg cgtcgcgaaa tggatgagtg
    24361 gatgtatgag tgaagtgccg gcaaccgatg ccgcggcgcc tcgcaaggcg gaaccggccg
    24421 tggtctatgc agcgcccgcc gacaaacgaa tttccgttgg cgcgccgagc ctgctggcat
    24481 tggctaacgg caagctgctg gtggctttcg accagaccgg gccggatgtc aaaggcctga
    24541 gcggcaaaaa aggcaacgac accaagcgtg gccgctggat gcaggggcgc gtgatggcct
    24601 ccaccgatgg cggcacttcg tggcaactgg ccgccaccta tccgttccgc cgcgccagcc
    24661 tcttccgcga ggggggcgat gtgtatttgt tgggcgaggc cgccggcggc ttgtgcctga
    24721 tgcgctcgcc ggacggcggc gccagttggt ctgcgccgat ggaactgacc ggggacttgg
    24781 acctgtggct cacgcccacc gccctgttgg ccacgaatga agcttggctg ctgccatgcc
    24841 tgattccggt ggaaaacaaa atggcgctca cgattttccg cgcgccacgc ggcgccagcc
    24901 tgatgcaccg caaggcgtgg acgcaggggc cgccctccgc gcccctcagc gacctgattg
    24961 ctgccgcgcc gggcgcgggc tgcggggttc ccgccggtcc cggtgcggtc acttggcgcg
    25021 agccggtgct gctggccatt agcgatggcc gccacccgtg gcacgatgag caagccgtcc
    25081 atgtggtcgg cgccaccagc agcgggcggc aacattgggc ggccttgttc cgcgtggcac
    25141 tgcccacttt gacaattgaa ccgcaacctg cgccaaccgg tgagccgtgg gtctggttgc
    25201 cgctgccggg ggggcacgac aagtttgatg tgttctacga cgcgcccagc cgcgcctatt
    25261 gggtggcggg cgcgcgcggc gtgccgggcc tgccgctggg gcgggaggtg gatcagccgc
    25321 aaggattgcg gcgcattggg ctttggactt cggacaacct cgcggaatgg gatacggtgg
    25381 ccacgctggt ggccggcggc gatggtcccg ccggcgtgcg ttgcgaccca gcgctggcgg
    25441 ttgccggcaa cgatctggtc tgggcctgtc gcgccggcgg cgagcacagt cgcaacgtcc
    25501 gtgaaaccac ccgcattttg tgcggccggc tgccgaattt tcgcgcgcgc tccgccgcgc
    25561 catgaggctg ctttttagga tgatttattc cccgctgctg ctagactggc cccacatcat
    25621 tttgggagaa gacaagggat gagcaatacg aagcaactgc cgacgtccga gcgccaagaa
    25681 cccccgcggc tgctgccgtt ccgctggctg gccgccattg ggctgggggg ggctgccctt
    25741 gttttgtatc tggctaccct gagccggggc gccttcccgg ggctgccggc caagagcctg
    25801 gtctggcaat tgcaattgga tatccgcccg ttgctgctgg attccttgtg gggctatctg
    25861 gtttgggcgc tgtcgtatct gcccggcggc tccgtggcgt tttgggttgg ggtgagcagc
    25921 gcggtattcg gcgcgctgtg cgtggtgctg ctgtcgctgc tgaccatgcg gctgcgctat
    25981 tcgctgcacg accagcacga tcccgcggaa gtccgccgcg aggcgcaggc cagcctgctg
    26041 gctggcgttg tggcgggact gtttctgatg ggcagtattc cgttttggat tctggccaac
    26101 cggtcgctgc ccggcgcgtt ccacctgctg cttctgctca gcgcgacgtt cctgttctcg
    26161 gaataccagc gcacggggcg ggtgggtttt ctctatgcgc tggggctgct ctacggggtg
    26221 ggcatcagcg aattcgccac gttctggatg tttgcgcccc tcaccgcgct gctggtggtg
    26281 cgcgccatgt tgcagcgcgc cgagttttcc atgcgcgtgc tggtgcgcac cgggctttgc
    26341 gtgctgcccg gcctgctgct gtatccgctg aacgcgtggt ggatgttcca agcccccggc
    26401 gtgagcttgc gcggctttgg ctcgctgtgg agcgtggtgt ggtacacctg gcgcgaccaa
    26461 tggcacctga ttgccaacgc cccgcaaacc tccggctttt tgctggtgat gtgcctgacg
    26521 gtgatcccgt ggggcatcct tttcctgatg cgctcgaaga agccggcatg gcggcattcc
    26581 gcgtggcagg ttttcttgcg cgtgctcgtg ctggcggtgg ccgtggccac gctttacgat
    26641 gccgccgtct cgccgtggca cttcttcggc atgcagtacc tgatggccac gccgtacgtg
    26701 attttggccg cttgcgcggg ttatgtggcc ggcgagtttt gggtgatggg gcagacccgg
    26761 gagcaccgca atgcgggcat cggccagccg ctgcgccggg tgatggccgg cgtgggcctg
    26821 ctgctgccgg tggccgccgt ggccgccttg gtgctcaacc agccggtggc ggatggccgc
    26881 tttggctccc gcctggaaag gctggccgac gaaacgctgg acgcgctgga cgggcgcgat
    26941 gtgctgcttt ccgacggcat catggacgac atgctgcgcc tgcgcgcttg ggaacgcggg
    27001 ctgccggtgc atgtggtgcc gctgcgcaac accgacaccc agctctaccg ccaatatctc
    27061 gcgctgactt tcaacagcga gcgccaacaa tcgctgctgc aaatcagttt tcccgccttc
    27121 atgcaggaat tcattgccgc cgatgaaaac ctgcgccggc tggcctccat tgactttacc
    27181 gacccgctgc gcgagtacgg ctatctggag cccgaccgca tgctctaccg cgccgaggtc
    27241 atggagcagg atattgatct gcccgcgctg gtggaacgcc aaaaaccgtt ctgggcggaa
    27301 atggaagagt gggccgcgct caacctggct gaccgcaatc cggcggccgg cttccggcaa
    27361 tatctgttgc ggctgacctc caaggtggcc aacaacatcg gctgcatgca ggtggaacgt
    27421 ggcgacaaaa ccggcgcgct ggccaccttc cagcaggccc gccggctctt cccggacaac
    27481 atcagcgcgt tgctcaatct gctgacgatt gcccaaacgg aaaagctgcc cgaagaagcg
    27541 gaatacaccg cgctctggga tgagttcaag gcgcggcaca tggatagccg tgtgatgtgg
    27601 gcgctctccg cgctctacgg ctatgtctac aacgctggct atctcgtgcg ccacggcatg
    27661 atgtgggcgg tttccggcaa gccacgcctg gccgaggccg aattgcgccg cgccgccggc
    27721 cgccaaggcg tgaatgccgc cgtgaaggcc ttcctcgggc gggcctattt gcagggccag
    27781 gacctgcaac gcagcacgga gtattaccgc gaggccatcc aggaaaatcc gcaagacgcc
    27841 cgctcgctgc tgatgctggc cgaaatcgcc ctgcgcgagg agcactacga cgaagcagag
    27901 gaatggctgc aaaaggtgga agccgccggc gtgcctgccg accggctgcg cttcgagcgg
    27961 acggccttga cgtatctgcg cggcgatacc gatcgcgcca tgcagcagtt gctggacctg
    28021 gtcaacaagg agaaagacaa cgtccgcgcg tgggcgctgt tggccatgct gaccagcgac
    28081 gcacctgacc ccacgttgca ccagcgcgca gtcaaggcgc tcaaggaatt gcagggcact
    28141 tcgaccgatg tgcgcttgct gctggccgag ctggacatca accagcaaaa ctgggctgcc
    28201 gcccgcgaca aactggaaca gttgacccgc accaacccgc gcaacgtccg tgcatgggaa
    28261 ttgctggtgg aagtggactt ccgcgagcgc aagcgcgact tggccgatga ccacgtgcgc
    28321 agcctgctta cgctgagccc gcaaaattat accggcaacc tcctgctggg ctcgctgcaa
    28381 tatgcccgcg gccaattctc gctggcggag tcgtcgtacc gcgcggcgct gcgcgtgcgg
    28441 cgcgaccccg ccgcgcttaa tgatttggcc tatctgctgt tgatcaacaa gccggccgcg
    28501 tggggcgagg cgcgggcgct ggtcgaagaa gcgcttgccg cgcaaccagc caacaagatt
    28561 gttttcctgt ccacccgcag cgagctgaac ctgcgcgaag gccggctgga tgaggccgag
    28621 gatgacctgc aacaggtgct caccgccatg cccaaccaag ccgcggccct gctgctggcg
    28681 gccgagctct acgccgcgcg cgcagccgag ctggcggcgg cggacgatgc cgccggcgcc
    28741 aagcgccagc aggcggcggc gctggatttg gccaactcgc tgaaagaccg gcaaaatgag
    28801 ttgtccttgg aacagcaaca gcacttgtat ggcttgctgg acgccaagcc gtaagttgag
    28861 caccacttgg cgcggggtgg gccggccggg ggtgggcgga gcccccggcg ttgggcgcgt
    28921 cacggaaccg caaccggagc catgagaaac aagccggaat ctgaatttta ttttttccgg
    28981 ccggacaagc gcctgctgcc gggtgatggc cgcgatccgc gcgtcccgtt gcagacggat
    29041 ccggtgatta acgaaacacc caccgacgtg cacttggcca ttgtgctctc ggtcatcatg
    29101 ttcgtgatgt atctggccag catgagttgg gagccgtttc ccgggccgcc gaccatcctg
    29161 ctgctgcgct gcctcgaccc cctccagccg ccggacaccc agaactttct ctggagctgg
    29221 atcatgaggg ggctggcgct gctgccgttt tcgaccgtgg ccattccggg tggcgtgttt
    29281 agcgcattgt gcggcgcgct gggcgtcggc attctcaccc ggttgatgat tcgcatcggc
    29341 tacgtcttgc gcaatgagcc cggccatttt tcttttctgc gcgaaatcca cgcccgacgg
    29401 ttgtccgggg tgaccgcggc actatttctt gggctgagca cacctttctg ggtggccgcc
    29461 acccgcaccc tgccggacac gtttgacctg ctgctgctgt tgctggttgc gtggtttatc
    29521 ggtgaatatc gccaccgggg gagaccgcgc gattgtctca aagccagctt gctgctgggc
    29581 gtgtgcctga cggaatatcc gagcgcgtgg gccttcctgc cggccatcct gttttttatg
    29641 tggcgcgaga tgtttcgttg gcgctcgctg cacaaatggt ggctgcatgc cttgattttt
    29701 ctggggctgc tggtggggct gctgctcctg ccgctcaatg cgtttttgct gttcaagcat
    29761 ttggcggtga tgcgcgccaa aatcacgccg gtcgaagcca tgttgcaggt gctcaaggac
    29821 cagtttttgc cgctggttta tttccgcttt agtccgggct tcctgccggt ggtggcgctg
    29881 gtgattgtgc cgtggctggc cattttcgtc atgtccacgc gctcgccatg gttctacgaa
    29941 tgggggcaag tgagcgtgcg cctgattttc atggccggct ttttcgcggt ggccttcaac
    30001 gcggcgtttg ccccgtggag cctcacggga atgtttttcc tgattgtggc gccctacgtg
    30061 ttgctggcgg tgtcagtggg ctacatcgcc ggcgaatttt ggatcatggg cgagccccat
    30121 gccctgctgg ataccgggtt cctcccccgc cttcggcggg gcgccatgag cctgattgcc
    30181 ttgctcttcc ctgtggtatt caccgccagc gcggtggtga actggcctgt ggtggatggc
    30241 cgctactcgc gtgatgccac ccggtttttc caatcggtgt tcacgcatct cggcgagcgc
    30301 gacatcctgt tttcctcggg ccaattggat gattccctgc gcctgctggc gctggagcag
    30361 catctgacca cgcgcattgt cagcgcgccg cataccggct cctccgtgta tctcaaccgg
    30421 ctggcgaatg ctttcgagga agaggaattg agcggcccgc tcgcgcaagg cgacttcggc
    30481 acattcctcg aaaacctgct gatgtccgag gatggcccca gccgcatcgg catcattgat
    30541 ttgcccgacg tgttccggga gtacggctat ctggtgccgg acaccatgct ctatcgcttg
    30601 gaagccaccc ccgatcgtgt ggactgggcc gcccttgccg ccagccagca gcccttctgg
    30661 gacaagatga tccgcatgac ccgcaacccg ctgccggaaa aaagcatcct gcgtgcgttt
    30721 gaaaattatc tgcgcgaaat ggtgtcgcgc gtggcaaata atctggccta tgtccaattg
    30781 gggcgcgggg atgatcaagc cgcgctggcc agcctgcgcg cagcgcatga aatctgggac
    30841 cacaatgtga gtgtgctgct gaacctgctg gaactggccc gcaagttaca cctgccagaa
    30901 caggaggatt gggaaaaaga attggacaag ctcaagccgg atttgggcga cgaacgctgg
    30961 gtgctcgcca tccgctacgg ctatgtctgg aatccgcgcg cgtggatgcg ccgcgggcat
    31021 gtgtgggcgc tttccggaat cccagccacg gaagaagccg cccgccgcaa gccggcggtg
    31081 tcggatgccg acgaagctgg caacgacaac cgcaaccggg tgctcgacca gtcctatctg
    31141 ctgtgggggc aggatgccgt ggatgaaacc ttcctgcgtg gccgactcgc caaaaatggg
    31201 cgcgacaccg atgcgctgat ggcgctgtgc cgcctcgcgc tgcgccggaa tgaccccgaa
    31261 gtggccgagg cctacatcat ggaggcgctg gattttggcc tcccggaggc caagacccag
    31321 tttgaccgcg ccatgctcgc gtttgtgcag ggcgataaaa cccgggcgat ccagatgctg
    31381 cgcgcgcaaa ccgtcagcac cccgcgcgac atgcgcgtgt ggacagcgct gttgctgctg
    31441 accgatgaaa ccgacccgat caacgtggaa gcgctcaaaa cactgaagaa ccaggcttac
    31501 gcacccgttg ggcaatatct ggcgctggct tccattcaca tgtcgcgcca gcagtgggcc
    31561 gatgcccagg aagagttgga ccgcgccatt gccttggatc cgcgcaatac gcaggcgtgg
    31621 gaaatgatgg tgcagttggc ccagcaacgc ggcaatcgcg ccctgttgca ggccggcctg
    31681 aaatgcctgc aagagcaaga ccccgaccac ttcctgcaat atcagaatgc cggagtggag
    31741 gcctaccaaa agggcaatct ggctctggcc gaggccgagt tccgcaaggg gctcatgcgc
    31801 cgacgcgacg cgttgctgtt gaacaatctg gcccacgtga tcatggagcg ccaaggcaac
    31861 atgaaagacg cgctcaagtt ggtggatgaa gcgctcctgc ggctccccgg ccaaccccag
    31921 atgttgagca cccgcgcgga aatctatatt aacctcggac gctatgcgga tgcgctccaa
    31981 gatttgcagg agtcattgcg caagcaaggt cgaaccaaca atctgctgct gctgctggcc
    32041 ctggcctatg aaggcttggg cgactggacg cgcgcggaaa ccatgagcaa aacactcgcc
    32101 ggcctgcaag cgcaactaac cccggcacag cgggaacgcc tggcgcaact gcaaggacga
    32161 ctggatgccc acaccgccgc caccgccgcc aaggccggcg aatatcagca ggaactcacg
    32221 cggttggaag ctgccctgcg cgcgcaaccg gacgatgccg ccttgcggac cgcgcgcgcg
    32281 accattctgc tgcaatgcgc gcgttacgat gatgcccggc tggatttgga agccgccgtg
    32341 cggagccggg gcgagacgcc ggagctgctg ttcctgttgg ttcaagcgtg ggaaggggcg
    32401 ggaaatccgc gccgcgcgct ggcctacgtc aaacggctgg ccagcatggc agaaacgctg
    32461 gatgatgccc agcgcgacca actccacgag ctgcggctgg ggctggaatt ggaactggag
    32521 ccctgatccc gctccggcct actggcgtgc agcagccgcc agcgccaaaa tgtcggcggg
    32581 ctgcaagctg gtgaaatccg gcacgaggcg gtccgcgccc ctcagctcgt cgcgcgtgtg
    32641 gctgctcaaa atcccgatgg ccttcatgcc ggcggcatgc gcggcggcaa ttcccgctgg
    32701 cgcgtcttca aacaccacgc attgcgcggg cggcacgccc agccccgctg ccgcctgcaa
    32761 gaagatatcc ggcgccggct tgccgcgttg gacatccgcg ccggaaatcg tgacgggaaa
    32821 ccatgcagta agtgcgaggg cctgcaagca cagatccaca ttctcgcgcg gcgcggagga
    32881 acccactgca caggggatgc ccagttggcg cgcagccgcc agaaaatcga gaacgccggg
    32941 gatcgggcgg atgccgtcgc ggcggatcca ttcgcggtag agttcttctt tttcgagacc
    33001 gaggcggtcg gcttcctctt cgctgaccgg ccagcgcagt aattcgcgga ttaccgcgcg
    33061 cgtacgcagc ccgcatttgc cgatgtactc cggatgcggg caggcaaacc cgtgccgttc
    33121 tgccacggct tgccaggagg ccacgtggag cccgttggag tccacaatga ctccatccag
    33181 atcgaagata aacgcgccgg cttgtgagtg ggccatgggt caggtcaatc cggccggcgt
    33241 aaagggcagc gggtagattt cgccgatggt cgtcacgctg atgtgccgga ttttgccctg
    33301 cttggtaagg ttgatcgcga taatccgcgc gtcggggccg gtggcaaatt cgcggatttt
    33361 ctggcggcac agaccgcagg ggagggcgta gccgctgctg gattgcaccg cgcaggccag
    33421 cgccttcaac tggaacgtgc cggtcttgat catgccgttg atggcatcca tctcggcatg
    33481 cgtcgtcagc gtgaagtcaa tcgtctcgat gttgcaaccc acgtggatgc ggttcttgat
    33541 gtcgcgcaat gccgcgccgc atttatactg cgaaaaaggc gcgtaggcct tctgacgaat
    33601 tgcgatggca gcctgcacca gccggcggtc gccggcggag agttcggaaa gggatacttt
    33661 tttcatggcg ggggactgta gcgcgccgcg gcaacgctgg caatctcaac gcacgaactg
    33721 ggcaaacact ttttccagcg cggcaatgtc cgtccagcca taggtctcga ggagcgcggt
    33781 gaggggatca tcgccgtcca gcagccggtt aaaaaactcg tcccatttgg ccatcggctg
    33841 cttgagttgc aggaagccca ccaaatattt gctcgtggca taaaattgcc ccacagcatc
    33901 caccttggga tcggtgttga gcgggtagct tgtggcgtgc agcaactcgg tgagcggcgt
    33961 ccaccgcgcc agcgggcgga acgcattgcg cttgccctgc cccatgccgc gcgccgcgcg
    34021 gtaggcaaac tcgccgtagt attccgccaa accttcgttg agccacagcg gcagccggat
    34081 cggcaaaaac cggttgaaaa ccagatgcgt catttcgtgg gcaagggtgc ccattttgtc
    34141 ggcggtgccg gtgccgattt cctgcaaata catcaccggg ccgcgcacga acgaggccgc
    34201 ccacgggtcc agccccggcg tgttggccac caccgccttc caactgcgcg cgtcgcggaa
    34261 aatgaaaata tgcgagcgca ccggcgagag ccggtctttc atttgcggta aatcggcgga
    34321 aatggcctga taaaattgtt caccgagccg cgccactttg gccgcaaaca ttttttggtc
    34381 gtggtgcagg atgaaatgtt cggtttgcaa gtgctgccac ttgtatgccg ggttgtcgag
    34441 caactcgata tcggccggca gaaggcccgt gcgttcgact tccgccgcgg tgatatcgcg
    34501 catggcgggc tggctctggc tgacatcgcg cgcgggcgcc gcgccgggta cggtgggcag
    34561 caccgggggc agggcccagc cggtctgcgt aaccaaaccc aacagcaacc cgcagaaaat
    34621 catccgcttt ttcatggcgt tcacctccag agcatcgtat atagtgggga acacgccatg
    34681 caagggaaaa cccaaaaagc accaacacct tgggctgtat ccggctcgct ggtggccttt
    34741 cccgccgatc gcgggcggca gacgcggctg ctggatggtt tctaccggcc cgggccgcgt
    34801 cgcggtgcgc cactgttggt gtttgttcac ggcatgggca gcaactttta ccgttccgcg
    34861 ctcaaaaaag ccttcctgca actcgctccc gaaaacgggt ttgccgtgct gtcctataac
    34921 aaccgtggtg cggagcgcgg caccgaagat gaacccttca gcgcctgctt ggctgatctg
    34981 gatgcggctg tcgagtttgg ccggcggcac gggcaccggc gactgttttt tgtcggccac
    35041 agcaccggct gccaaaaaat tgttttttgg caatcgcgtc gccgcgcgcg tgcggtgtcg
    35101 ggtctggtgt tgctggcgcc ggcggacgac tatgccatca cccggcaggc gctgggccgg
    35161 cagttcgatc gcaaagtggc ctgggcgcgc aaaatgctgg ccgccggcaa gggctcggcg
    35221 ctgattcagg cgctctatga gcggttcact gccaaacgat ttctgagcat cgccaatccg
    35281 cgcgccattg aagccaatat ttttcgctat gccggtccgc tgacccattt ccgccgcgtc
    35341 aaagt
//
LOCUS       DAOPNP010000009        33279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1735_length_33279_cov_8.811281, whole genome shotgun sequence.
ACCESSION   DAOPNP010000009 DAOPNP010000000
VERSION     DAOPNP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 33279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33279
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_33279_cov_8.811281"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKI64_01785"
     CDS             complement(<1..369)
                     /locus_tag="PKI64_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99769.1"
                     /translation="MRRWLKPSPCWPSPAAPLRRNAAQRAGGGGCKNQPFGALARLKL
                     GVEDFLRILAAPKPTGIFSALARRDRPLADTFEEFRVQSSGFRGGKKLAPAVPFVLFS
                     FHAPFLFSCYFLFSCLVRWLF"
     gene            complement(332..1447)
                     /gene="hisC"
                     /locus_tag="PKI64_01790"
     CDS             complement(332..1447)
                     /gene="hisC"
                     /locus_tag="PKI64_01790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019110162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOD99770.1"
                     /translation="MSLFTPIANSWIANQRPYQPGRPLEEVARELGLPSIEGIVKLAS
                     NENNLGPSPRAMAAMREAIPHMHLYPDGGAFYLRQAIAKKFGVADNMVMMGCGSNEHI
                     VLLAHAFMQEGDNLVCSARSFVVYKLVAALYRCQCIEAPMQGMTHDLDAMLRAITPRT
                     KLVIVANPNNPTGTLVDQAAVDRFIEQVPDHVVTVFDEAYMELVEPEMYVDTVKHVKA
                     GKKPVVTLRTFSKAYGLAGLRVGYGIAQPDGIDLLNKVRQPFNVTAMALAAALAALDD
                     DEYLAASRRMLKEGRAQLMAGMRRLGLDPVESVTNFILFPFAKAGELTAELTKRKVIV
                     RPMAGFGLPDYVRVSVGKAEENAQFLAALAEALAVLA"
     gene            complement(1444..2241)
                     /gene="scpB"
                     /locus_tag="PKI64_01795"
     CDS             complement(1444..2241)
                     /gene="scpB"
                     /locus_tag="PKI64_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOD99771.1"
                     /translation="MNDFVLPELKQIIGAMLFVRKEPLTVAEIRRVLQATAERRGGIT
                     SDFAKAGEALIREAVDELSRDLRDRKVGFHVTEVAGGWKLENDANCGPWLRVMLQKGR
                     GARLSLPALETLAIIAYRQPCVRSEIEAVRGVAVDAILRNLLEMQLVRVVGRSELPGR
                     PWMFGTTQKFMEHFGLKSLADLPGTDELRRMEAAQARARAPQAAAEPPPEDPNQLKLP
                     AIEAEAPETPSADEAIGTVSDEEEDTFARDDMSADEIIGGEDEETEA"
     gene            complement(2246..3031)
                     /locus_tag="PKI64_01800"
     CDS             complement(2246..3031)
                     /locus_tag="PKI64_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOD99772.1"
                     /translation="MTQEEYKVRLDVFEGPLDLLLYLIKKDELDIYNIPIERITTQYV
                     QYLDLMKMLDLNIAGEFLVMAATLMMIKSRMLLPVEERPELEEEEEDPRWDLVRQLVE
                     YKKFKDAALHLESLEARREDIFWRDGAEAVLTKEADLALRDVGLFDLISAFNDALKKI
                     QNEELREIFAEHFTVAEKIEQLSDRLARERSFSLSDMFAAMRSRQEIACTFLALLELI
                     RLSQARAVQRETYGEIFIERAEGVVELVPPVVDQAAPEPRGKE"
     gene            complement(3088..4050)
                     /gene="trpS"
                     /locus_tag="PKI64_01805"
     CDS             complement(3088..4050)
                     /gene="trpS"
                     /locus_tag="PKI64_01805"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOD99773.1"
                     /translation="MRILSGIQPSGKLHIGNYFGMMRPAIELQQRGEAFLFIADYHAL
                     TTVADAAAMREGVRDVALDFLACGLDPERTAFYRQSDLPEVHELAWLLSNVTPLALLE
                     RCHSYKDKLAKGIAPNHGLLAYPVLMAADILIVQANVVPVGRDQKQHVEVTRDLAIKF
                     NLAFGDTFTIPEPQIADAVAVVPGLDGQKMSKSYGNTIEIFGDPKATRKRIMSIVTDS
                     KTLEEPKDPDTCNVFRLYKLFATEAQQADLAARYRAGGMGYGTAKKELADLLETFFAP
                     MRAKRAELAANPRYVEDVLQAGAAKARAVARDTLQRARRAVGLE"
     gene            complement(4175..4627)
                     /locus_tag="PKI64_01810"
     CDS             complement(4175..4627)
                     /locus_tag="PKI64_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOD99774.1"
                     /translation="MSDAADLYQELILQHSRTPRHFGPLPQATHTAAGDNPLCGDRYQ
                     IRVQVDENGIIRDGAFEGVGCAISKASGSLLLDAVIGHSRDEFERLFQDFHAVVRGDE
                     QSPAAIARLGKLAAFAGIWKYPARVKCAILCWHALRGALAGEHSATTE"
     assembly_gap    4642..4741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4742..5686)
                     /locus_tag="PKI64_01815"
     CDS             complement(<4742..5686)
                     /locus_tag="PKI64_01815"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOD99775.1"
                     /translation="MNKPAPQPLDPVAIRREFACYRQAGAPLVYLDSASSSLKPDCVI
                     DAVANHYRQTPANIHRGLYPMSEAATAAYENAREQVRRFINAAAREEVIFTSGTTDAI
                     NLVAHGFGNALQAGDEILITGLEHHANIVPWQLLRERRGIVLKVVPITPAGEVDPTDF
                     RAALGPRTRLAAFTAVSNALGTETPVAELVALARAAGAATLVDAAQAMACCPVDVQAW
                     GCDFLAFSGHKLFGPNGVGVLYGRRERLEAMNPFKGGGDMIRAVTYERTTYNDLPYKF
                     EAGTPPIAAAVGLGAAIQWVEELGLPRIATHEAALVAAA"
     gene            complement(5679..6962)
                     /gene="sufD"
                     /locus_tag="PKI64_01820"
     CDS             complement(5679..6962)
                     /gene="sufD"
                     /locus_tag="PKI64_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOD99776.1"
                     /translation="MKTRFVGLGEGYAALFNAQRGGVLDPPWLAGRRDDAFARLQQSG
                     LPTRHVEEWKYTDISFLAGQTPAAPVGDAVAVDPARLPMWHEDDLNIVLVDGRLQQEL
                     SPALESGLLEWARLGDLGKNPETTPVAAHPAALLHAAFVHDGVLVRIPANAQLARRVH
                     ILLLHASLTPHALLAPWVAIFAGANSQARVVITAYSLSDAACLHLPRVMVSADSESNL
                     AISQAQLLNTQSWQLATSRVELQRDAHVAHLEAGLGAALARHDLTAVLLESGARVELN
                     GLYALDGKRHADFHTLIEHRQPNCSSRQVYKGILAGKAAGVFNGAVRVFPGASGTDGY
                     QLNKALLMSREAQMFSKPELLIDNDDVKCSHGATIGQINPQELFYLQSRGIPEAQARH
                     MLARGFVEDIMFQQGDDRLRADLDGALDRYFAAHE"
     gene            complement(6959..7720)
                     /gene="sufC"
                     /locus_tag="PKI64_01825"
     CDS             complement(6959..7720)
                     /gene="sufC"
                     /locus_tag="PKI64_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOD99777.1"
                     /translation="MLRIKKLKARVKDGPEILHGITLTVNAGEIHAIMGPNGSGKSTL
                     ARILAGHPDYEVTGGDVHFQVNGEWQDLLGMEPHERAKAGVFLAFQYPVDVAGVSNVT
                     FLLAAYNAIRRARKEPEADAFEFRELLVEKAALIDLDPSYLDRAVNLDFSGGEKKRNE
                     ILQMAVLEPTLAVLDETDSGLDVDSLRIVAEGVNRLHAAHRAVLMITHYQRMLNYITP
                     QFVHIMMDGRLVRSGDAQLAHEVENRGYDWLKETA"
     gene            complement(7731..9182)
                     /gene="sufB"
                     /locus_tag="PKI64_01830"
     CDS             complement(7731..9182)
                     /gene="sufB"
                     /locus_tag="PKI64_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOD99778.1"
                     /translation="MANKIQTEQLQQRGREYKYGFETVIEQDTFPKGLNEDIVRAISQ
                     RKGEPDYLLEFRLKAYRWWAQQPAPTWAAVEFPPIDFQDIRYFSAPKSMARPVNEAGE
                     VDPILVRTFQRLGIPLREQERLTGVAVDAVFDSVSVGTTHQKMLAEHGIIFCSFSEAA
                     RNHPELVQKYLGSVVPPTDNFYAALNSAVFSDGSFVYIPKGVKCPIDLSTYFRINARE
                     TGQFERTLIIADVGAEVSYVEGCTAPARPENQLHAAIVELIALDDATIKYSTVQNWYG
                     GDAAGRGGIYNFVVKRGRCAGKRSKISWTQVEAGAAITWKYPSVVLLGDDSVGEFYSV
                     ALTNNQMQADTGTKMIHIGKNTRSTIVAKGISCDQSKNSYRGLVRIAPGAENARNYSQ
                     CDSMLVGDKAAANTFPYLEVSNPSAIVEHEATTSRISAEQLFYLQSRGLDTEAAVSLL
                     VNGFCKDVFKTLPLEYMVEAVNMLELKLEHSIG"
     gene            complement(9305..10624)
                     /locus_tag="PKI64_01835"
     CDS             complement(9305..10624)
                     /locus_tag="PKI64_01835"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOD99779.1"
                     /translation="MTPTNEAILNALRAVIDPDFEKDIVTLGFVKDLAIRDGVVSFKI
                     ELTTPACPIRDQFKEEAERVVKAVPGVTSVHVTMTAQERPMRKLAQGSGLAKTTCILA
                     VTSCKGGVGKSTVAAMLAVNLARRGFRTGLLDVDIFGPSAPTLFNLHEKGVETTDEDY
                     IVPREWENLKVMSFGFALGDEPAVMRGPMVSGLVQQFLHQVAWGELDYLIIDFPPGTG
                     DVQLTISQSVQLDAAVIVTTPHELSLADVRKGIQMFAKVNVPVLGVIENMAYFQCPGC
                     GERHEIFGKSGTDLLRERFGLDNLAVLPVRTALSGRLEDLAAAPEAAAATDAVMRALG
                     RQMMARPERPKPVATPTHIELHWSDGRVERVANRELRLACGCALCVDEMSRVPLLDPD
                     TVPEDIHAEEVWTIGNYALGIAWSDGHATGFFPFQTIHQLAEATPAE"
     gene            10746..11801
                     /gene="nadA"
                     /locus_tag="PKI64_01840"
     CDS             10746..11801
                     /gene="nadA"
                     /locus_tag="PKI64_01840"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOD99780.1"
                     /translation="MKTNPISQSTDVRAIEAWMMQHLRHLVPEIEIRAKAELAAQINR
                     LKTERDAVILGHNYMEPALYHAVADIRGDSLELCREAQKADRSVIVFCGVEFMAETAK
                     ILNPTRKVLIPSTKAGCSLAESITADDVRRLKAQFPGVPVVAYINTYAAVKAETDICC
                     TSSSAAKIVASLDTDTVIFIPDEYLARNVARETGKTVIFPEQAAAACPSANTFIGWHG
                     KCEVHDLFTVQDVENVRRQFPQAVILAHPECPPAVAAAADFSGSTSAMVRFVQEHPAP
                     QYFLMTECSMADNIAAENPNKEMIRLCSHRCPYMATITLEDTLAALEHLRYEVTVPED
                     IRLRAKKSIDRMLALTT"
     gene            complement(12196..15168)
                     /locus_tag="PKI64_01845"
     CDS             complement(12196..15168)
                     /locus_tag="PKI64_01845"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOD99781.1"
                     /translation="MKTWVTFIGCVLLAAAMIGLVATARWHNQCANEAVAECETEIRQ
                     YLVEELGIAALFTFEDPQARDLISVSAALCPGTVAVVQEGRPARQFDGREQTWLAWPL
                     PWDMLGSNFTLSAWIRLEEPERDQDIFGGAYATLQTGLRYENGQIFFDVPAKERQCVS
                     YPFTRTGEFVHVAATVDMASGRMSLYENGELKAEGPVKEILWPPLNLCAGKTSWYVVR
                     SPFSGMIAETALWNRPLSAAEIQKLSSGVGSLLATHVPRALRWRHRLAKRWLAVTQTF
                     CTVQNVFDSRLWRATEKSIAGVPAIRLVLSDKDRKHFLNAHEQSRRSGRRVLQAARPR
                     RVHALAEGAMLSAQLWLHGSDTAYADFIRPGFVLEYEDGCTFDGCSKILLQPPEASGF
                     LAPFVISRLERHFGVPQVRDGLCRLYINNTLAGIYRYSDYRQNGIMPGDYRRAAEPFI
                     RTPTTWNVMFNTQPFEPALRIKSRPWPGDQALLDTLFAEVEQECRPLLLRDFQSPLNT
                     REIGQQIRQNYYDLQEQMHPAWAAGPPAQHMADFLDEYLVLGSNRSPFYLEGDLDLSV
                     HQVADTSLAWESSAPQWISTTGKVHQPTSGAPKEVVLTAVIQDPQQTIRKDLRFRVMP
                     RETTLPAVFLTVTDILAKDRRVDAVVEFADPTSGIRRLIASQAHRAGVNHRGNTSYWG
                     KKKLLSIKTDSAHGVFGDPQDRVLKTINARQDPSFLNNGLAYGLFREFSAPGAPRYAP
                     LVRPSEVFVNGHYYGLYELCTRIDERLVGETPWDGEETPDIFVYRHESAPPRAHPFRV
                     SRPRGAEHSWHEPIDQLLNDFTKAEWLPRISARHDISSLIDLQILINLMQNRNGYPFP
                     FAMHDALVYDATRGQLFHVPWDFDHPNTRPWEWLANNMMRRMEQENPDYMPTLAARWF
                     ALRQTVLSDHALERRIQALRDPIAAYVRWDYERWNYSHGVPHRRHVQHTRNVLRNSTA
                     AMDAALKKWLPHYPATKR"
     gene            complement(15525..16823)
                     /locus_tag="PKI64_01850"
     CDS             complement(15525..16823)
                     /locus_tag="PKI64_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOD99782.1"
                     /translation="MKTSKGWNHMRMMLAQPRGAGLQTVRRYVWRLLLLGALLAGPAR
                     AQLITSNDWAVGYSLPTQSAEVFLKGEYVIRDMLLAGINRLSNGNSAAIATFTFSGDS
                     TETGAAGPILQALDHALDRGAKVHFVVDSAVKRFKEYTPGLSLTRLSKRRRNPLVLSV
                     SPRNSLMHHKVAIFDYGPSNRWVLVASGNFTGAANMNQWNIALLARNEELYGAFAAEM
                     AEFKARRFGAQKQQQHDRTNFRLQDSWGTCWVRFGPYPKGTNGAVNAETDIRRLIHDA
                     EEEIYFSMHRFNRVPLRRDLVAAANRGIKVVGVIPESDRGTNLFATSKATARYFADPA
                     NYTGTNRVVLLPARASAISDAWDSGEPDLVHTKYAIIDPNGRRPFVVHGAANWTISGL
                     VAPDGNDENVLILRHRDIAQAFLAHFQRVTATTPPPKPAE"
     gene            17015..18562
                     /locus_tag="PKI64_01855"
     CDS             17015..18562
                     /locus_tag="PKI64_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99783.1"
                     /translation="MSNDKQALPGHETQSHEMNCINCGRFVGAVSKCPYCGAKVEKRM
                     SLVAVRWAAVLLATIGLFLLYLMAKYREIPVVKLGDVTPTMNFAQIRIEGQAQSDART
                     FRNGGMGFTVSDGTGVIMVFMSPKQAKDLAASDRVPKAGDKVNFAGGLSVAEDEASVR
                     LISTKDFQITRAPAAAVRLANITDAMVGKSIVIAGKVVDVFPPPANTKRPYALKIQDD
                     SGEQTVNFWQSEYDQIQGKDVLNGAYVRLRVSVDTYQDKIQLKLAAGGDLEILDGPPT
                     EAYSSPGKKAAATYAKKKATAATPARDFSRGRSVQSNSLSVSEVTTALDGKTVRVRGR
                     VDSVKPPQEGTKQPFAVMLKDKDAALRVTYWSAVDEVIPVKPTPGTLFEMEGVVEVYQ
                     DTPQLKVKSGYKVKLVDDAPASAPAVDVSRAVPVSSITAANKGETRVVQGTLGVPRAL
                     RGGVAYTLTDDSGSIDLVLWESLVPTDVLGALAEGVKVAVSGEVGEYEGQLQLKADRG
                     YSAMVIP"
     gene            18577..19911
                     /locus_tag="PKI64_01860"
     CDS             18577..19911
                     /locus_tag="PKI64_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOD99784.1"
                     /translation="MSVFWIALFATLAGTVIASLLACLPGLHIYNVMGALVLAIIALA
                     DTQWAIAPEIYLPMMAAMIVGWSMVNTIPAVLLGTPDESAMFTVLPGQKYLMDGRGFE
                     GTMITAVGGLAGAFFLILVVAPFAPRYLPVSQEVLKGHMHWVLWIIITFMLMSEWPKG
                     GNRGPAGWAKFYDAWKTLLAGLATFFLAGLLGFIILTRSPVSHEVAFQNIMPAFVGLF
                     AIPWCLLNIISAADMPKQTVTKSLAVNGDIILRSVWSGSLGGGLAAYFPIVTGGVGGM
                     LAGHATAQRDERIFIMGQGVSKFIYFVGSFILMFVPGLHLTRGGGAWMAKTLYTPSGI
                     SDYYLILGSIAVASAVAYLLMEPLTKLTLKFISRYNYRHVSSVALAIIILMVWGITGW
                     IGVFIMAVGTGIGLLPVLFGSRRLNCLGILLFPIACNMSGFGETITGWLGLT"
     gene            19927..20958
                     /locus_tag="PKI64_01865"
     CDS             19927..20958
                     /locus_tag="PKI64_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOD99785.1"
                     /translation="MKGFSHFMSGVAVASFGPWAIEAAQQGNPIFFILGGACGILPDT
                     IDFKFYRFFYEHDVYITPDPLNPDPQYVANEFARAVALAVDEKRYVRVKLVSVRLGAD
                     FWQQYSVKIDNEKLEVQVKFGPVVNTGQVPVKGTEDRYPTVATAKLKAKVIQTYDAAL
                     KVDIFDGPTIGFKPMDNGDLDLEFLPWHREWSHSLTVGAILGVLLGAVAWWASGWTMA
                     WQVFVTIAACYGVHVVEDQLGHMGSNIFYPLTKNRTPGLHWMHSGDGLPNFLAVWISC
                     LFIFWNLYRGVPKPTYHFSFIHLMMVGLVIPGLIFWGLRKLLTLGQNVQMKKGDDADD
                     EWNESKAAG"
     gene            21103..23580
                     /locus_tag="PKI64_01870"
     CDS             21103..23580
                     /locus_tag="PKI64_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOD99786.1"
                     /translation="MRFQRAIFCRLCLMAGLWAGGATLTVHAGGGAFNTLVVVNTNSA
                     ESVELGEYYASQHGIPTHQICSVGIATNTATLTPTNFFTLLRDPILAHIAAENLTGQI
                     DYVVLCGDLPTRVRDVEGVTASLFYGFQNAPSVWEVGACNLPLYTTNNYYRAERAFRS
                     VAGWNNTNGFVAFHLLGTNLANAKLVADRGVAAQSTFPAGTIYLYNLGDGPRGWREQL
                     FGDAQFSFTALPGLPAECFIAAPWATLNNKTNVMGYHDGCAYLTAGAKSTNNTWLAGA
                     YADHMTSYGGRLLDTNHNQNTVLDWFGIGATASYGTVAEPCSYIEKYPDPFLGFLYAR
                     GFTIGEAYAMAVFAPYQGLFAGDPLAAPYAAPPVITVSQPTNNQIVTGTVTVAISATA
                     HARGVPAAGFDLFVDNRLRTNLVTLAPTPGNQLAVAVAGRTNSVTVAANDTLFDVVNA
                     LADAVNNDATQTVYATAFGDRLELIHRQFDHAGDNAAVSATVAQGTADSLTLGVGLAA
                     TNIAPSPYHARKMVWLATRTGSGANANDKLTCIITLTNGVAITNTLVATQGEAVTNIL
                     ERLRQAINTNSTLQATNGVSYAHNSQYPVNYGYLTARTPGPDGYGIVVDYRVTAVSNT
                     TGLATNSDMTTFLDDNARDTRARASILFHVRPTNGTLAAEYALDTTALADGHHTLDII
                     ARDGSAVAAQSRQTLTIFVANGVTSQGTPHWWLAQHGWTNDFENAALADTDNDGVPAW
                     QEYLADTDPTDADSFPWLEFLDVLQDPPVLTWPASPARAYQIHYADDLFNGPWVTQQL
                     LQGASEWADTNPPPATNRFYRLFPHLP"
     gene            complement(23603..25507)
                     /locus_tag="PKI64_01875"
     CDS             complement(23603..25507)
                     /locus_tag="PKI64_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOD99787.1"
                     /translation="MDLLTVVLVILYLMLTGYLGYLGYIQTKNATDYLVAGRSAHPAV
                     MALSYGATFISTSAIVGFGGVAANFGMGLLWLTVLNIGVGIFVAFVFLGNPTRRMGHH
                     VDAHTFPELIAKRYDSRFLQVACALIIFCFMPLYAMAVMVGGAEFIGPIFHISYDSAL
                     YIFALIVAAYVIAGGLKGVMMTDALQGGIMLVGMIILLVATYGRLGGVTPAHQALGAM
                     HDQVPPFLKAIGHQGWTAMPKFGFAAPQTPMPGAVEYHLWWILVSTIVMGVGIGVLAQ
                     PQLIVRFMTVKSKQALNRAVVAGGVFILFMTGVAFTVGALSNVYFAQQEAIRCRIVEE
                     AAWLDPGADGKGKLVVLPATADEETLARAKRFVSFRLPDESDEAPLNYVLWTPQMEIE
                     RGADGALDVVRPHLVAALRSAGPGTTLQGNADAIIPAYVRHAMPRWFGILFLLTLLSA
                     AMSTMSSQFHTMGTALGRDVFAKYSQKRGSQSIWVTQLGVVAGIVVALVMGKYVRGNI
                     IALATSIFFGLCASSFLPMLVFGLFWRRMTKPAAVASLLVGFLTSGFWLLFVNGKTAA
                     GLGLCKAWFGKDHLVPTHWSLTWTVVDPLVVALPLSVLTAVVVALVTKPMDPAYVRYV
                     FGGPRPGEDD"
     gene            complement(25523..25687)
                     /locus_tag="PKI64_01880"
     CDS             complement(25523..25687)
                     /locus_tag="PKI64_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99788.1"
                     /translation="MVLGIEDPGVWLAYVLSLACAALCVGYGIIHWNKGLEQPAKPDD
                     DDDEPTGVDL"
     gene            26137..28776
                     /gene="alaS"
                     /locus_tag="PKI64_01885"
     CDS             26137..28776
                     /gene="alaS"
                     /locus_tag="PKI64_01885"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOD99789.1"
                     /translation="MMTAAQIRQSFLDFFASKDHEIVPSSPVVLPTDPTLLFANAGMN
                     QFKEIFLGARKSSWKRVANTQKCIRVSGKHNDLEEVGRDTYHHTFFEMLGNWSFGDYY
                     KREAITWAWELLTVVWKLPKNRLWATVFTTDDEAADIWRTCTDIDPSHILRFGEKDNF
                     WEMGETGPCGPCSEIHFDLTPDGCTPDMINAGSPLVIEIWNNVFMQYNRKSDGSLDEL
                     PAKCVDTGMGFERVVAVIQGKTSNYDTDVFMPLIEAVAALSGKAYTEGENAVAMRVIA
                     DHLRALSFAIADGVMPGNEGRGYVLRRLLRRAARYGRNLGLTKPFLCELFPVLEKQMG
                     GFFPELTARRNEIVRALRAEEESFAATLDRGIAHFNEVIEKLGSAGNKLFPGSEAFKL
                     YDTYGFPLDLTVLMAQEKGFQIDAPAFERFMAEQRARARAARKDNAAADNDLIAHFVE
                     QGLQTTFTGYTSLTTGTSLVALLADGQIVDTLATGQTADLLLAQTPFYAEAGGQVGDT
                     GVITTCQGARFEVTDTQKPAQGIILHRGKLVAGTLAKGAAVVATVDEERRAAIRRNHT
                     ATHLLNAALRKVLANDSLKQAGSYVDAERLRFDFTSFEALTPAQLAAIERQVTEFVIA
                     NRAVKTYEIPLKDVPGSGIIAVFDEKYGDIVRVVETEGISRELCGGTHVERSGDIGPF
                     RILAETSIAAGIRRIEAQAGLPACDHTAHEHGILSDLAHRLSVAPADLPPRLEALQQH
                     AHQLEKELKALAQKNALEKGAALAAKFAPIGGIPVLIESVGELEAEPLRALVESLRKQ
                     TPESVILLAATAEDKVTFILNAGPAAQAKGVHAGKLVGAIAKLTGGGGGGKPDKAQAG
                     GKDAAKLPAALAHARELIAQALG"
     gene            28880..29716
                     /locus_tag="PKI64_01890"
     CDS             28880..29716
                     /locus_tag="PKI64_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOD99790.1"
                     /translation="MPTPIIAVANQKGGVGKTTTAVNLAACLAERRKKILLVDLDPQA
                     NATSGLGLAKTEGASIYDSLLGDRPLQEIIQPSADSRLDVVASEVDLAGAEIEVARAE
                     HHLLRLAMALAPLAAQNHYDFIFVDCPPSLGILTMNALHAADGLLIPMQCEYYALEGI
                     SVILNLVHKLHDSGAAPRLAVEGIVMTMYDMRTNLSQQVVGEIRTHFPDKIYDSLIPR
                     SVRLAEAPSHGLPIIAYDPNCTGAAAYRQLAREFLKRRKTAVEPAGADAPPAPAPDAP
                     PA"
     gene            29716..30543
                     /locus_tag="PKI64_01895"
     CDS             29716..30543
                     /locus_tag="PKI64_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99791.1"
                     /translation="MEAAPTIMAATGHLRVCVLASGSSGNCIYVESPTTAVLIDAGLS
                     ARETLRRLTAIGREPARIQAICITHDHSDHVAGVPRLHKLHGWELYANGDTARAVDPK
                     QQLPWRLFTAGSRFQIGDIELLPFSLPHDAYDPVGFVLTHGRSRLGIATDLGMATQLA
                     RHHLRGCHLLVLEANHDEVMVKNSFRPWELKRRILSRQGHLSNETAAELLVDVAGDTL
                     QQVFLAHLSSECNNVDLALHTVRTRLTAAGFGHVHVRPTHPDRPSEFGELFFDNPAG"
     gene            30545..32266
                     /gene="argS"
                     /locus_tag="PKI64_01900"
     CDS             30545..32266
                     /gene="argS"
                     /locus_tag="PKI64_01900"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOD99792.1"
                     /translation="MQTIQDLLSEQVRAAFAAAFPGADLAGLPLAATPAGDEAFGDYQ
                     SNGAMAAAKVLRQPPRQVAEQVVRHLPTGDLLAKVEIAGPGFINMTLADDALARHVAA
                     MQDDPHVGLPQSGAGRRLILDYSAPNVAKPMHIAHIRSTVIGDALKRIYRALGYEVLG
                     DNHLGDWGTQFGILIMGYRHFLDKAALAASPVDELQRVYVLSHQRCEENPAWQDQARA
                     ELVKLQAGDPENRALWQTFIDLSLEEFNRIYARLGVSFELIRGESYYNDALPGVVQKL
                     QDLGLVRESEGALVAFLDDEKLPPCIVRKSDGGYNYATTDLATVFSREQEFQPAAILY
                     VTDERQQLHFRQFFAIARKAGVTTPLRHIWFGLMRLPEGLLSTRKGTAIKLDLLLDEA
                     EKRALELVRQASPDMPEAQQQAVARAVGIGAVKYADLSQNPQSAVSFTWEKALAMDGN
                     SAPYLQYAYARIASVLDKHAERFPGHDWCAAPLVLDSPHARRLALRLARYSGAVCAAA
                     AQEKPSLLADALYDLSQAYSSFYQNVPFLKAEAGLRESRVRLCALTAKVLRHGLGLLG
                     IETPDRI"
     gene            complement(32638..33108)
                     /gene="rplQ"
                     /locus_tag="PKI64_01905"
     CDS             complement(32638..33108)
                     /gene="rplQ"
                     /locus_tag="PKI64_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOD99793.1"
                     /translation="MRHRKNTVKLGRTTAQRSALMASLVCNLILAKRIKTTLPKARAA
                     KRLADKMVTLGKKGTLAARRQAIATLRLEKAVQELFSAVAPAMAERKGGYTRVIKLGK
                     RMSDSSEMCLLEWVDFVPPEAKAKGKKDAKEPKKDDKKEAKKDSKKTSKKDDKK"
     gene            complement(33128..>33279)
                     /locus_tag="PKI64_01910"
     CDS             complement(33128..>33279)
                     /locus_tag="PKI64_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOD99794.1"
                     /translation="QKTENEMLKYRNFGKKSLNEIKQKLAELGLSLGMTFDAELLNMT
                     QPAEE"
BASE COUNT         6247 a        10736 c         9998 g         6198 t
ORIGIN      
        1 aaacagccac cgaaccaagc aagaaaacag gaaataacag gaaaacagga aaggggcgtg
       61 gaaagaaaat aggacgaacg ggacggctgg ggctaatttt ttgccgcccc tgaaccctga
      121 actctgaacc ctgaactctt caaaagtgtc cgcgaggggt cggtcgcggc gcgccagcgc
      181 ggaaaagatg ccggttggtt ttggcgccgc caaaatccgc aagaaatcct ccaccccaag
      241 cttcagccgt gccaaggccc caaaaggttg atttttgcag ccaccgcccc ctgccctctg
      301 cgccgcgttg cggcgcagcg gggcggcggg gctaggccag cacggcgagg gcttcagcca
      361 gcgccgcaag aaactgcgcg ttctcctccg ccttgcccac gctgacgcgc acatagtccg
      421 gcaggccaaa gccggccatc gggcgcacaa tcactttgcg cttggtgagt tccgccgtca
      481 gctcgcccgc cttggcaaag gggaacaaaa taaagttggt gacggattcc accgggtcca
      541 accccaaccg acgcatgccg gccatcagtt gggcgcggcc ttctttcaac atccggcgcg
      601 aagccgcaag atactcgtca tcgtccagcg ccgccagcgc ggcggcgagg gccatggcgg
      661 tgacgttgaa tggctggcgc accttgttga gcaggtcaat gccgtcgggc tgcgcgatgc
      721 cgtagcccac gcgtagcccg gcaagaccgt acgccttgga aaacgtgcgc agggtgacca
      781 ccggtttttt gccggctttg acgtgcttga cggtgtccac atacatttcc ggctcgacca
      841 attccatgta ggcttcgtca aagaccgtga cgacatggtc cggcacctgc tcgatgaacc
      901 ggtccacggc cgcctgatcc accagcgtgc cggtggggtt gttgggattg gccacaatga
      961 cgagcttggt ccgcggggtg atggcgcgga gcatggcgtc gaggtcgtgc gtcatgccct
     1021 gcatgggcgc ttcgatgcac tggcagcggt agagcgcggc cacgagctta tagaccacga
     1081 acgagcgggc agaacacaca aggttgtcac cttcctgcat gaaggcgtgg gccagcagaa
     1141 caatgtgctc gttggagcca cagcccatca tgaccatgtt gtcggccacg ccgaactttt
     1201 tggcgatggc ctgccgcaaa taaaacgcgc cgccgtcggg atacagatgc atgtgcggga
     1261 tggcttcacg catggcagcc atggcccgcg gcgaggggcc cagattattt tcgttggagg
     1321 ccagcttgac gatgccctcg atgctgggca accccagctc gcgtgccact tcttccagtg
     1381 gacggcccgg ctggtacggg cgttggttgg cgatccagga attggcaatg ggggtgaaca
     1441 ggctcatgct tcggtctcct cgtcttcgcc gccaatgatt tcatcggcgg acatatcgtc
     1501 gcgcgcgaag gtgtcttctt cctcgtcgga gaccgtgcct atggcttcat cggcgctggg
     1561 ggtctccggc gcttcggctt cgatggccgg caacttcaac tggttggggt cttccggcgg
     1621 cggctccgcg gcggcctgcg gcgcacgggc gcgcgcctgg gcggcttcca tccggcgcag
     1681 ctcatcggtg ccgggcagat ccgcgaggct tttcaggccg aagtgctcca tgaacttctg
     1741 ggtggtgccg aacatccacg ggcggccggg caattcgctg cggcccacca cgcgcaccaa
     1801 ttgcatttcc agcaggttgc gcaaaatggc atccaccgcc acgccgcgca cggcttcgat
     1861 ttccgagcgc acgcagggct ggcggtaggc aatgatggcc agcgtttcca gcgccggcag
     1921 cgagagccgc gcgccgcgcc ctttttgcag catcacgcgc aaccatgggc cgcagttggc
     1981 atcattttcc agcttccagc cgccggccac ttccgtcacg tggaaaccca ccttgcggtc
     2041 gcgcaggtcg cgggagagct cgtccacagc ctcgcggatg agggcctcgc cggccttggc
     2101 gaagtcgctg gtgatcccgc cgcgccgttc cgcggtggct tgcagcacgc ggcggatttc
     2161 cgccacggtc agcggctcct tgcgcacaaa aagcatggcg ccgatgattt gtttcagttc
     2221 gggcaacaca aaatcgttca tccgttcatt ccttcccacg cggctccggc gcggcctggt
     2281 ccaccaccgg cggtaccaac tccaccacgc cttcggctcg ctcgatgaaa atctcaccgt
     2341 aggtctcgcg ctgcacggcg cgcgcctggc tcaggcggat cagctccagc agcgccaaaa
     2401 aggtgcaggc aatctcctgc cgcgagcgca tggcggcaaa catgtccgag agcgaaaagc
     2461 tccgttctcg cgccagccgg tcgctcaact gctcgatctt ctcggccacg gtgaagtgct
     2521 cggcaaaaat ctcgcgcagc tcctcgttct ggattttctt gagcgcgtca ttgaaggcgc
     2581 tgatcaaatc aaagaggccc acatcgcgca gggcgaggtc ggcctccttg gtgagcacgg
     2641 cttcggcgcc gtcgcgccag aaaatatcct cgcgccgcgc ctccagcgat tccagatgca
     2701 gcgctgcgtc cttgaacttt ttatattcca ccaactggcg cacgaggtcc cagcgcgggt
     2761 cttcctcttc ctcttccagt tcggggcgct cctccactgg cagcagcatg cggctcttga
     2821 tcatcatcag cgtggcagcc atcaccagaa actctccggc gatgttcaaa tccagcatct
     2881 tcatcaggtc cagatactgg acatattgcg tggtgatgcg ctcgatgggg atgttgtaga
     2941 tgtccagctc gtcctttttg atcaggtaga gcagcaggtc cagcggccct tcaaacacgt
     3001 cgaggcggac tttgtattct tcctgcgtca tgcgaatccg cctgcctgct ccgcgttccc
     3061 aatcattcca tccggcggct gccgggctta ctccagcccc accgcgcggc gcgcccgctg
     3121 caaggtatcg cgcgccaccg cgcgcgcctt ggccgccccc gcttgcagca catcctcgac
     3181 atagcgcggg ttggccgcca gctccgcgcg cttggcgcgc atgggggcga aaaaggtctc
     3241 cagcaaatcg gccagctctt tcttggcggt gccgtagccc atgccgccgg cgcgatagcg
     3301 cgcggccagg tcggcctgct gcgcttccgt ggcaaacagc ttgtagagcc ggaagacgtt
     3361 gcaggtgtcc ggatccttgg gctcctccag cgttttgctg tcggtgacga tgctcatgat
     3421 ccgcttgcgc gtggcttttg ggtcgccaaa aatctcgatg gtgttgccgt agctcttgga
     3481 cattttctgg ccgtcgaggc ccggcaccac ggccacggca tcggcaatct gcggttcggg
     3541 gatggtgaag gtgtcgccaa aggcgagatt gaacttgatg gcgagatcgc gcgtgacttc
     3601 cacgtgctgt ttttggtcgc gccccaccgg caccacgttg gcctgcacaa tcaggatgtc
     3661 cgccgccatc agcaccgggt aggccagcag gccgtggttg ggcgcgatgc ccttggccag
     3721 tttgtcctta tacgaatggc accgctccag cagcgccagc ggggtcacgt tcgagagcag
     3781 ccaggccagc tcgtgcacct cgggcaaatc gctttggcgg taaaatgcgg tgcgctcggg
     3841 atcgaggccg cacgccagaa aatccagcgc cacatcgcgt acgccctcgc gcatggccgc
     3901 ggcatcggcc acggtggtca gcgcgtggta gtcggcaatg aacaaaaacg cctcgccgcg
     3961 ctgctgcaat tcgatggccg gccgcatcat gccgaaatag ttgccgatat gcagtttgcc
     4021 cgacggttga atgcccgata gaattctcat cacgccactc tggttcgtac ctcaaaaaat
     4081 cccgcgcatc ctaccccccg ccccgccccg cgcaaagcca aaagaacacc ccgcgcagca
     4141 tcgcgcgggg gctgtctggc cccgtctgcg gcagctattc ggtggtggcg gaatgctcgc
     4201 ccgcgagcgc gccgcgcagg gcgtgccagc agagaatcgc acatttgacc cgcgctggat
     4261 atttccagat gcctgcgaac gccgccagtt tgcccaaccg cgcaatggcc gcgggtgact
     4321 gttcgtcgcc acgcaccacg gcatggaaat cttggaacag ccgctcaaac tcgtcgcgcg
     4381 agtggccgat cacggcatcc agcagcagcg agccggaagc cttggaaatg gcgcagccca
     4441 cgccctcgaa cgcgccgtcg cggatgatgc cgttttcatc cacctgcacg cggatttggt
     4501 agcggtcgcc gcagaggggg ttatcgcccg cggcggtgtg cgtggcctgc ggcaacggcc
     4561 cgaaatgccg gggcgtacgg ctgtgctgca agatcagctc ctgatacaga tcggcggcgt
     4621 cgctcatccg agaatctcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nggcggcggc caccagcgcc gcttcgtggg tggcaatgcg tggcagtccc agctcctcca
     4801 cccactggat ggccgcgccc agccccaccg cggcggcaat gggcggcgtg ccggcctcga
     4861 acttgtaggg caaatcattg taggtggtgc gctcatacgt caccgcgcgg atcatatcgc
     4921 cgccgccctt gaaggggttc atggcttcca gccgctcgcg acgaccatac agcaccccca
     4981 ccccatttgg cccaaacaac ttgtgccccg aaaacgccag aaaatcgcag ccccacgcct
     5041 gcacatccac cgggcagcac gccatggcct gcgcggcatc caccagcgtg gcggcgcccg
     5101 cggcgcgggc caaggccacc aattccgcca ccggcgtttc cgtgcccagc gcattcgata
     5161 ccgccgtgaa tgcggccagc cgcgtgcgcg ggcccaacgc tgcgcggaag tccgtggggt
     5221 ccacctcgcc cgccggcgtg atgggcacca ccttcagcac gatgccgcgc cgctcgcgca
     5281 gcaattgcca cggcacaatg ttggcgtgat gctccaagcc ggtgatcaag atttcgtcgc
     5341 ccgcttgcag cgcgttgccg aacccgtgcg ccaccagatt gatggcatcc gtggtgccgc
     5401 tggtgaaaat gacttcctcg cgcgccgccg cattgatgaa acgccgcacc tgctcgcgcg
     5461 cgttttcata ggcggcggtg gcggcttcgc tcatggggta cagcccgcgg tgaatgttgg
     5521 cgggtgtctg gcggtaatgg ttggctacgg cgtcaatcac gcagtcgggt ttcagcgagg
     5581 acgacgcgct gtccagatag accagcggcg cgccggcctg ccggtagcag gcaaactcgc
     5641 ggcggatggc caccgggtcc agcggttgtg gagcgggctt attcatgggc ggcaaaatac
     5701 cggtcgaggg ctccatccaa atcggcgcgc aggcggtcgt cgccctgctg gaacataatg
     5761 tcctccacaa agccgcgcgc gagcatgtgg cgcgcctgcg cctcgggtat gccgcggctt
     5821 tgcagataaa acagctcctg cgggttgatc tggccgatgg ttgcgccgtg cgaacatttg
     5881 acatcgtcgt tgtctatcag cagctccggc ttgctgaaca tttgcgcctc gcggctcatc
     5941 agcagcgcct tgttgagctg gtagccatcg gtgccggacg cgccgggaaa cacccgcacc
     6001 gcgccgttga acacaccggc tgctttcccc gccagaatac ccttgtacac ctgccggcta
     6061 ctgcagttgg gctggcggtg ctcgatcagc gtatggaaat ccgcgtgccg cttgccatcc
     6121 aatgcgtaaa gcccgttcag ctcgacccgc gcgccggatt ccagcagcac ggcggtcagg
     6181 tcgtggcgtg ccaacgccgc gcccagcccg gcctccagat gggccacgtg ggcatcgcgc
     6241 tgcagttcca cgcggctggt ggccaactgc cacgactgtg tgttcagcag ttgcgcctgg
     6301 ctaatggcga gattcgattc ggaatcagcg ctgaccatca cccgcggcaa gtgcaggcag
     6361 gcggcatcgc tcaacgagta ggccgtgatg accacccgcg cctgcgagtt ggcgcccgca
     6421 aaaatggcca cccacggcgc cagcagcgcg tgcggggtca ggctggcatg gagcagcagg
     6481 atgtgcaccc gtcgcgccag ttgcgcattg gcggggatcc gcaccaacac gccgtcgtgt
     6541 acaaacgccg cgtgcaacag cgccgccgga tgcgccgcaa ccggcgtggt ttcggggttc
     6601 ttgcccaaat cgcccaagcg cgcccactcg agcaagccgg actccaacgc cggcgaaagc
     6661 tcttgctgca atcggccatc caccagcaca atgttcagat cgtcctcgtg ccacattggc
     6721 aggcgcgcag ggtccacggc cacagcgtcg cccaccggcg cagccggtgt ctggccggcc
     6781 aggaaggaaa tatccgtgta tttccactcc tcgacatggc gcgtgggcaa gccactttgc
     6841 tgcagccggg caaaggcatc gtcacggcgg cccgccagcc agggcgggtc cagcacgccg
     6901 ccgcgctgcg cattgaacag cgccgcgtag ccttcgccca gcccgacgaa acgggtcttc
     6961 atgcggtctc cttcagccag tcatagccgc ggttttccac ctcgtgcgcc aactgggcat
     7021 cgccggagcg cacgaggcgt ccatccatca taatgtgcac aaactgcggc gtgatgtagt
     7081 tcagcatgcg ctggtagtgc gtgatcatca gcaccgcgcg gtgcgccgcg tggagccggt
     7141 tgacgccctc cgccacgatg cgcagcgaat ccacatccag cccggagtcc gtttcgtcca
     7201 gcaccgccag cgtgggctcc agcaccgcca tttgcaggat ttcgttgcgc ttcttttcgc
     7261 cgccggagaa atcgagattc accgcgcggt cgagatagga cgggtccaaa tcaatcagcg
     7321 ccgctttttc caccagcaat tcgcggaact cgaacgcatc ggcctccggc tccttgcggg
     7381 cgcggcggat ggcgttatag gccgccagca ggaaggtgac gttgctgacg cccgccacat
     7441 ccaccgggta ctgaaacgcg aggaacacgc ccgccttggc gcgctcgtgc ggctccatcc
     7501 cgagcaaatc ctgccattcg ccgttcacct ggaaatgcac atcgccaccg gtgacttcgt
     7561 agtccgggtg acccgccaga atccgcgcca gcgtactttt gccggagccg ttcggcccca
     7621 tgatggcgtg gatttcgccg gcgttcaccg tcagcgtgat gccgtgcaag atttccgggc
     7681 cgtccttgac gcgggccttc aactttttga tgcgcaacat gtcctgctcc ttatccgatg
     7741 ctgtgttcca gtttcagctc cagcatattc acggcctcca ccatgtattc cagcggcagc
     7801 gtcttgaaca cgtccttgca aaagccattg accagcagcg acaccgcggc ctccgtgtcc
     7861 agcccgcgcg actgcaaata aaaaagctgc tccgcgctga tccgggaggt ggtcgcctcg
     7921 tgctccacga tggcgctcgg gttgctcacc tccaaatacg ggaaggtgtt ggccgccgcc
     7981 ttgtcgccca ccagcatgga gtcgcactgc gaatagttgc gcgcgttctc cgcgcccggc
     8041 gcaatccgca ccaacccgcg gtagctgttc ttcgactggt cgcaggaaat ccccttggcc
     8101 acgatggtgc tgcgcgtgtt tttgccgatg tgaatcatct tcgtgccggt gtccgcctgc
     8161 atctggttgt ttgtcagtgc caccgaataa aactcgccca cggaatcgtc gcccagcaac
     8221 accacgctgg ggtatttcca ggtgatggca gcgcccgcct ccacctgcgt ccaggaaatc
     8281 ttggagcgtt tgccggcgca ccgcccgcgc ttgaccacaa agttgtagat gccgccgcgt
     8341 cccgccgcat cgcccccata ccagttttgc accgtggaat atttgatggt ggcgtcgtcc
     8401 agcgcaatca gctcgacaat cgcggcgtgg agctggtttt cgggccgcgc cggcgccgtg
     8461 cagccttcca cgtaggacac ctccgcgccg acatcggcaa tgattagcgt gcgctcgaac
     8521 tgccccgttt cgcgcgcgtt gatgcggaaa taggtcgaga ggtcaatcgg gcatttgacg
     8581 cccttgggga tgtacacaaa cgagccgtcg gaaaacaccg ccgagttgag cgcggcgtaa
     8641 aagttgtcgg tcggcggcac aacgctgccc agatatttct gcaccagttc cggatggttg
     8701 cgcgccgcct cggagaacga gcagaaaatg atgccgtgct ccgccagcat tttctggtgg
     8761 gtggtgccga cggatacgct gtcaaacacc gcatccaccg ccacgcccgt cagccgctct
     8821 tgttcgcgca gcggaatgcc cagccgctgg aatgtacgca ccagaatcgg gtccacctcg
     8881 ccggcctcat tgaccggccg cgccatggat ttgggcgcgg aaaagtagcg gatgtcctga
     8941 aaatcaattg gcgggaactc caccgccgcc catgtgggcg cgggctgttg cgcccaccaa
     9001 cggtaggcct tgaggcggaa ctcgagcaga taatccggct cgcccttgcg ctgggaaatc
     9061 gcccggacaa tgtcttcgtt caggcccttg gggaaggtgt cctgctcaat gaccgtctcg
     9121 aagccgtatt tatactcgcg gccgcgctgc tggagctgct cggtttgtat cttgttcgcc
     9181 atggttttag ttccggtggt tcacgccaat gtaggcccgc ggaggccgcc ccgcaaatca
     9241 cgggccgcat ttttctggcg atgacccggc aaaaacaggg gcgcggaagg ggggcgggcg
     9301 gcggttattc cgccggcgtg gcctcggcaa gctggtggat ggtctggaac gggaagaagc
     9361 cggtggcatg tccatccgac cacgcaatcc ccagcgcgta gttgccgatg gtccacactt
     9421 cctcggcgtg gatatcctcc ggcacggtgt ccgggtccag caacggcacg cggctcattt
     9481 cgtccacgca caacgcgcag ccgcaggcca agcgcagctc gcggttggcc acgcgctcca
     9541 cgcggccatc gctccagtgc agctcaatat gggtcggcgt ggcaactggc ttcggccgtt
     9601 cggggcgggc catcatttgc cggccgaggg cgcgcattac ggcatccgtg gcggcggcgg
     9661 cctcgggcgc agcggccaaa tcttccagcc gcccgctcaa cgcagtgcgc actggcagca
     9721 ccgccaagtt gtccaggccg aagcgctcgc gcagcaggtc cgtgcccgac ttgccaaaaa
     9781 tctcgtgccg ctcgccgcag cccggacact ggaaataggc catgttctca atgacgccca
     9841 gcaccggcac gttcaccttg gcgaacatct ggatgccctt gcgcacgtcc gccagcgaaa
     9901 gctcatgcgg tgtggtcaca atcaccgccg catccaactg gaccgactgc gaaatagtca
     9961 actgcacatc ccccgtgccc ggcgggaaat caatgatcag gtaatccaac tcgccccagg
    10021 ccacctgatg caaaaattgc tgcacaaggc cggagaccat cggcccgcgc atcaccgccg
    10081 gctcatcgcc tagcgcaaag ccgaacgaca tcaccttcag gttttcccat tcgcgcggca
    10141 caatgtagtc ctcgtccgtg gtttccacgc ctttctcatg caagttgaac aacgtgggcg
    10201 ccgaaggccc gaaaatatca acatccaaca ggcccgtgcg gaagccgcgc cgcgccaaat
    10261 tcaccgccag catggccgcc accgtggact tgcccacccc ccccttgcag gaggtcactg
    10321 ccaaaataca cgtggttttg gccaggccac tgccctgcgc cagcttgcgc atcgggcgct
    10381 cctgcgccgt catggtcacg tgtacgctcg tcacgcccgg cacagctttg accacgcgct
    10441 ccgcttcttc cttgaattga tcgcggatgg ggcacgccgg cgtggtcagt tcaatcttga
    10501 acgacaccac cccatcgcga atggctaaat ccttcacaaa gcccagcgtc acgatgtcct
    10561 tttcaaaatc gggatcaatc accgcgcgca gcgcgttcaa aatggcttca ttggttggtg
    10621 tcatggtggt ccttactgct tggccgcgcc tccgcgggcg cgttcgtcac aacctagccg
    10681 gatgaacatg atttgtcaaa aaccacactc aaatgtagag tgggcgggat aggtggaaaa
    10741 aggccatgaa aactaatccc atttcgcaaa gcacggatgt ccgcgccatc gaggcgtgga
    10801 tgatgcagca cttgcgccac ctcgtccccg aaattgaaat ccgggccaag gcggaactgg
    10861 ccgctcaaat caaccggcta aaaactgaac gcgatgctgt gattttgggc cacaactaca
    10921 tggaacccgc gctttatcac gccgttgccg acatccgtgg cgactcgctt gaactttgtc
    10981 gcgaggccca aaaggccgat cgcagcgtca tcgtgttttg cggggtggaa ttcatggcgg
    11041 aaaccgccaa gattctcaac cccacgcgca aagtcctcat cccctccacc aaggcgggct
    11101 gctcccttgc tgaaagcatc actgccgatg acgtccgccg cctcaaagcc caatttcccg
    11161 gcgtcccggt cgtcgcctac atcaacactt acgctgccgt taaggccgag accgatatct
    11221 gctgtacgtc cagtagcgcc gccaaaatcg ttgccagcct cgacacggac acggtgattt
    11281 ttattccgga tgaatacctc gctcgcaacg tcgcgcgcga aaccggcaaa accgtgatct
    11341 tccccgaaca ggccgccgcc gcctgcccca gcgccaacac cttcatcggc tggcatggca
    11401 aatgcgaagt ccacgacctg ttcaccgtgc aggatgttga aaatgtccgc cggcaattcc
    11461 cgcaagccgt cattctcgcc caccctgaat gcccccccgc cgtggcggca gccgccgact
    11521 tttccggcag cacctccgcc atggtgcgtt ttgtgcaaga gcaccctgcg ccgcaatact
    11581 tcctgatgac cgaatgctcg atggccgaca acatcgccgc cgaaaatccc aacaaggaga
    11641 tgatccggct atgcagccac cgctgcccct acatggccac catcaccctc gaggacaccc
    11701 tcgccgccct cgaacacctg cgctacgagg tcaccgtccc cgaggacatc cgcctgcgcg
    11761 ccaagaaatc cattgaccgc atgctcgccc tcaccaccta gcgccactgc gcgccatttg
    11821 cgagttcatc gttgttttca gtggtcggat agtgccgcct agggtgagga aataaccgtc
    11881 gtcaaaccaa cctcttggtt ttgtcatttg aatcatgcca tccagagttt ataaactctg
    11941 ggcatatgtt gtaatatccc catcccattt atacacaatt tattacatct attttttagg
    12001 caattttaaa ttggacattt tgagggtact gtccacagtt tataaagtct ggacatattt
    12061 ttggtgagtg atttttgcgt ttttatccga gtggttccgc atgggaggcg ccaaacccaa
    12121 gacccctact aaaaatcagc gaaggttcca tttgcggcgt tggcggcgct tgcgtttact
    12181 ggtcttcggc tgctgctatc tttttgtcgc gggatagtgg ggcagccatt ttttcaaggc
    12241 ggcatccatg gcagcggtgc tgttgcgcag cacgtttcgg gtgtgttgca cgtgccggcg
    12301 gtgcgggacg ccatggctgt agttccatcg ctcgtaatcc cagcgcacat acgctgcgat
    12361 tggatcgcgc aacgcttgga tgcgacgctc aagcgcatgg tcggacaaca ccgtttgtcg
    12421 cagcgcaaac catcgtgcgg ccaaggtggg catgtagtca ggattctctt gctccatgcg
    12481 acgcatcatg ttgttggcca gccactccca aggccgggtg ttgggatggt caaaatccca
    12541 tggcacatgg aataattgcc cgcgggtggc gtcgtacacc aacgcatcgt gcatggcgaa
    12601 tgggaatgga tagccgtttc ggttttgcat gaggttgatg agaatctgca agtcaatgag
    12661 gctgctaatg tcgtgccgcg ccgaaatacg cggcaaccat tccgctttcg tgaagtcatt
    12721 cagcagttga tcaatcggct catgccagga atgttcggcg cccctgggtc gggacactcg
    12781 gaaagggtgc gcgcgcggcg gcgcgctttc gtggcggtac acgaagatat cgggagtctc
    12841 ctctccatcc cacggtgtct cacccacaag gcgctcatca atccgagtgc ataattcgta
    12901 aaggccgtaa tagtggccgt tcacaaacac ctcgctaggt cggacgagcg gcgcgtagcg
    12961 cggcgcgcca ggcgcagaaa attcgcggaa caggccatag gccaacccat tgttcaaaaa
    13021 cgaaggatcc tgccgcgcgt tgatggtttt tagaacccgg tcctgcgggt caccaaaaac
    13081 gccatgggcg gaatcggttt tgatggaaag taatttcttt ttgccccaat aactggtgtt
    13141 gccgcggtga ttgaccccgg cacggtgggc ctggctggcg atcaggcggc ggataccgga
    13201 tgtgggatcg gcgaactcca ccacggcatc cacccggcga tccttcgcca ggatatccgt
    13261 gacggtcagg aaaaccgccg gcaaggtggt ttcgcggggc atcacccgga aacgcaggtc
    13321 cttccggatg gtttgttggg gatcctgaat gacggcggtc agaaccactt ctttgggcgc
    13381 gccggatgtg ggctgatgaa ccttgcccgt cgtggagatc cattgcggag cggaactttc
    13441 ccacgccagt gatgtatccg caacttgatg gaccgacaaa tccaagtcgc cttccagata
    13501 gaacggcgag cggttggagc cgagtactag atactcatcc agaaaatccg ccatatgctg
    13561 cgccgggggg cctgcggccc aagcggggtg catttgttcc tgcaagtcgt agtaattctg
    13621 ccggatctgc tggccgatct cgcgggtgtt caaaggactt tgaaaatcgc gcaacaacag
    13681 cggccgacac tcttgctcaa cttctgcaaa aagagtgtcc aacaacgcct gatcgccggg
    13741 ccatgggcgt gacttgatgc gtagcgcggg ttcgaatggt tgcgtgttga acatcacatt
    13801 ccacgtggtt ggagttcgga tgaatggttc ggcagcccga cggtagtcgc cgggcataat
    13861 gccgttttgc cgataatccg agtaacggta aattccggcc aacgtgttgt tgatgtaaag
    13921 ccggcagagg ccgtccctaa cctgtggcac gccgaagtgg cgttccaatc gggaaataac
    13981 aaaaggcgcg agaaaaccac ttgcctcggg cggttgcagc aggattttgg agcagccgtc
    14041 gaaggtacag ccgtcctcat attccagcac aaacccaggg cggatgaagt ccgcgtaggc
    14101 ggtgtcgctg ccgtgcaacc aaagctgggc ggaaagcatg gcaccctccg ccagcgcgtg
    14161 aacacggcgc ggccgggcag cttgcagcac gcgtctgccg gaacgacggc tttgctcgtg
    14221 ggcgttgagg aaatgtttac gatccttgtc gctcaacacc agccggatgg ccggcacccc
    14281 ggctatgctt ttttcggtgg cacgccacag gcgcgaatca aaaacattct gaaccgtgca
    14341 gaatgtctgc gttactgcca gccaccgttt ggccagccga tgacgccacc ggagggcgcg
    14401 ggggacgtga gtcgcaagca ggctccccac cccgctgctc agcttttgaa tttccgccgc
    14461 ggatagcggg cggttccaga gcgcggtttc cgcaatcatg ccgctaaacg gcgaccgcac
    14521 cacgtaccaa gaggttttgc ccgcacacaa gttgaggggc ggccagagaa tctctttgac
    14581 gggcccttcc gctttcaatt cgccgttttc atacaagctc atgcggccgg acgccatgtc
    14641 cacagtggcc gcaacatgca caaattcgcc cgttcgggtg aacgggtagc tgacgcactg
    14701 gcgctctttg gccggcacat cgaaaaatat ctgcccgttt tcataacgca gccccgtttg
    14761 caacgtggcg tatgcgccgc cgaaaatgtc ctgatcccgc tccggctctt ccagacgaat
    14821 ccaagcggag agagtaaaat tggaccccag catatcccat ggcaagggcc aggccagcca
    14881 ggtctgttca cggccatcga actgacgtgc aggccggcct tcttggacaa cagccacagt
    14941 tcccgggcac agagcggcgc tgactgaaat gaggtcccgg gcttggggat cctcaaatgt
    15001 aaacagcgcg gcgatgccga gttcttcgac aagatattgc ctgatctccg tttcgcactc
    15061 tgccacggcc tcgttggcgc attggttgtg ccatcgcgca gtggcaacca acccgatcat
    15121 tgccgcggca agcagaacac aaccgatgaa tgtcacccag gttttcaccc acgccgccgg
    15181 gttgatcaac ctgctacgag tggggagggc tcttgcgcgc cgggcgaagg aatcaaaaaa
    15241 gctttggggc cggctggaac gagcgtattc atgcgtggca tggtaggcgg cacggaatgc
    15301 gaccgtcaat gccgaacgtc tcacagacct tccagccttt cccggcaaga aggtttcgtc
    15361 ccgcaacaag tcccgtccta caaaagagtc ccaatgtgaa gttcagaaag cagggccatt
    15421 tttcaaggtc atggattgtg accttgatca gaaggatggg ctaataattg gttcctgagt
    15481 aaacctcaat cgaattgccg tggtgcgatt gggaggctga ggttttactc ggcgggtttg
    15541 ggcgggggtg tggtggcggt cacgcgctga aagtgggcaa ggaaggcttg ggcgatgtcg
    15601 cgatggcgca ggatcaggac gttttcatcg ttgccgtcgg gcgcgacgag gccggagatg
    15661 gtccagttgg cggcgccatg caccacgaac gggcggcgtc cgttggggtc aatgatggcg
    15721 tatttggtgt ggaccagatc gggttcgccg gaatcccagg catcgctgat ggcgctggcg
    15781 cgggcgggca gcagcacgac gcggttggtg ccggtgtagt tagcggggtc ggcaaagtag
    15841 cgggcggtgg ccttggaggt ggcaaacagg ttggtaccgc gatcgctttc ggggataacg
    15901 ccaacaacct tgatgccgcg gttggcggca gcgaccagat cgcggcgcag gggcacgcgg
    15961 ttgaagcggt gcatggaaaa atagatttct tcttcggcgt cgtgaatcag gcggcggatg
    16021 tcggtttcgg cgttgacggc gccgttggtg cctttggggt agggaccgaa acgcacccag
    16081 caggtgcccc aactgtcttg cagccggaag ttggtgcggt cgtgctgctg ctgtttttga
    16141 gcgccaaagc ggcgggcctt gaattcggcc atttcagcgg cgaaggcgcc gtagagttcc
    16201 tcgttgcggg ccagcagggc gatattccat tggttcatgt tggctgcgcc ggtaaagttg
    16261 ccggaagcca ccagcaccca gcggttgctt gggccgtagt cgaagatggc cactttgtgg
    16321 tgcatgagcg agttacgggg cgagacggac aacacgagcg ggttgcggcg gcgtttggag
    16381 aggcgtgtga gggagagccc cggcgtgtat tccttgaacc gcttgacggc gctatccacc
    16441 acaaaatgga cttttgcgcc gcggtccaag gcgtggtcca acgcttgcag gataggcccg
    16501 gcggcgccgg tttcggtgga gtcgccggaa aacgtgaagg tggcaatggc cgcgctgttg
    16561 ccattggaga ggcggttgat gccggcgagg agcatatcgc ggatgacgta ttcgcctttg
    16621 aggaaaactt cagccgattg ggtgggcagg ctatagccga cggcccaatc gttgctggta
    16681 atcaactggg cccgggccgg cccggccagg agcgccccca gcaggagcag gcgccagacg
    16741 taccgccgga ctgtttggag gccggcgcca cggggttgtg caagcatcat ccgcatgtga
    16801 ttccaccctt tgcttgtttt catcagcgca ccggtgattc tccacggaac cgcgcgggat
    16861 ggaaaggatg aaaatggcgc ggcgggggtc ccggcgcaaa aacgttccac ccgccggatg
    16921 cttttgtttt ttgcggtttg ctgtcgaaca ggctaggttg acagatgccg tttttttggt
    16981 tccccttgcg gcaggggttt ggaggtaatg gattatgtcg aacgacaaac aggcgctccc
    17041 cggccacgaa acccaatccc atgaaatgaa ttgtatcaac tgcggccgct ttgtcggcgc
    17101 ggtgagcaag tgcccgtatt gcggcgccaa ggtcgaaaag cgcatgagcc tggtggccgt
    17161 gcgctgggcg gcggtgctgc tggccaccat cggccttttc ctgctctatc tcatggccaa
    17221 gtaccgcgag attccggtgg tcaagctggg cgatgtgacg ccgaccatga actttgcgca
    17281 aatccgcatt gaggggcagg cgcaaagcga tgcccgcacc ttccgcaacg gcggcatggg
    17341 ctttacggtg tccgacggca ccggcgtgat catggtgttc atgagcccca agcaggccaa
    17401 ggatttggcg gccagcgacc gcgtgcccaa ggcgggcgac aaggtcaact tcgccggcgg
    17461 gttgagcgtg gcggaggatg aagcctccgt gcggctgatc agcaccaagg atttccaaat
    17521 tacgcgagcg ccggctgcgg cggtgcgcct ggccaacatc actgatgcca tggtgggcaa
    17581 atccattgtg attgcgggca aagtggtgga tgtgttcccg ccgccggcca acaccaagcg
    17641 cccctacgcg ctcaagattc aggatgacag cggcgagcaa accgtgaatt tctggcagag
    17701 cgaatatgac cagattcagg gcaaggacgt gctcaacggc gcgtatgtgc ggctgcgcgt
    17761 ttcggtggac acctaccagg acaagattca gctcaaacta gccgcggggg gggatctgga
    17821 aatcctcgat ggcccgccga ccgaggccta tagctcgccg ggcaaaaagg cggcagccac
    17881 ctacgccaag aaaaaggcca ccgctgccac tccggcgcgc gatttctccc gcgggcgctc
    17941 ggtgcagtcg aacagtttgt cggtcagcga agtgaccacc gcgctcgatg gcaagaccgt
    18001 ccgcgtgcgc gggcgggtgg actccgtgaa gccgccgcag gagggcacca agcaaccgtt
    18061 cgcggtaatg ctcaaggaca aggatgccgc gctgcgcgtg acgtactggt ccgccgtgga
    18121 cgaagtcatt cccgtcaagc ccacgccggg cacgctgttt gaaatggaag gcgtcgtgga
    18181 agtctatcag gacacgccgc agctcaaagt gaagagcggc tacaaggtca aactggtgga
    18241 cgatgccccc gcctccgccc ccgcggtgga tgtctcccgc gcggtgccgg tgagttccat
    18301 caccgccgcc aacaagggcg aaacccgcgt ggtgcagggc acactgggcg tgccgcgcgc
    18361 gctgcgcggc ggcgtggcct acaccctcac cgacgactcc ggcagcattg atctggtgct
    18421 ctgggaatcg ctggtgccca ccgatgtcct cggcgcgctg gccgagggcg tcaaggtggc
    18481 cgtcagcggc gaagtcgggg aatatgaagg ccagctgcaa ctcaaggcgg accgcggcta
    18541 ttccgccatg gtgatcccct aaccggaggc ccgatcatgt ccgttttttg gattgcgctt
    18601 tttgccacgc tcgccggcac ggtgattgcc agtttactgg cctgcctgcc gggcctgcac
    18661 atttacaacg tgatgggcgc gctggtgctg gccatcattg ccttggctga tacccagtgg
    18721 gccatcgccc cggaaatcta tttgccaatg atggctgcaa tgatcgtggg ctggtccatg
    18781 gtcaacacca tcccggcggt gttgctgggc acgccggacg aatcggccat gttcaccgtg
    18841 ctgccgggcc agaaatactt gatggatggc cgcggctttg aaggcaccat gatcaccgcc
    18901 gtcggtggct tggccggcgc gttcttcctg atcctggtgg tggcgccgtt tgcgccgcgc
    18961 tatctgcccg tttcacaaga ggtgctcaag gggcacatgc actgggtgct gtggatcatc
    19021 atcaccttca tgctgatgag cgaatggccc aagggcggca accgcgggcc cgcgggttgg
    19081 gccaagttct acgatgcgtg gaaaaccctg ctggccgggc tggccacatt ctttctggcg
    19141 gggctgctgg gcttcatcat tctgacccgc tcgccggtca gccacgaagt ggccttccag
    19201 aacatcatgc ctgcgttcgt gggcctgttt gcgattccgt ggtgcctgct caatatcatt
    19261 tccgcagccg acatgccgaa gcaaaccgtc accaagagcc tcgccgtcaa tggcgacatc
    19321 attttgcgtt cggtttggtc gggcagcctg ggcggcgggc tggcggccta cttcccgatc
    19381 gtcaccggcg gcgtgggcgg gatgctggcg ggccacgcca cggcgcagcg cgatgagcgc
    19441 atcttcatca tgggccaggg cgtctccaag ttcatttact tcgtgggctc cttcatcctg
    19501 atgttcgtgc cgggcctgca cctgacgcgc ggcggcggcg cctggatggc caagaccctt
    19561 tacaccccca gcggcatcag cgactactat ctcattctgg gcagcattgc cgtggcctcc
    19621 gccgtggcct atctgctgat ggagccgctg accaagctca cgctaaagtt catcagccgc
    19681 tacaactacc gccacgtatc cagcgtggcc ctcgccatca tcattctgat ggtgtggggc
    19741 attaccggct ggatcggcgt attcatcatg gccgtcggca cgggcatcgg cctgttgccg
    19801 gtgctgttcg gctcgcggcg gctcaactgc cttggcattt tgctgttccc cattgcttgc
    19861 aacatgtccg gttttggcga gaccatcacc ggttggctcg ggctcaccta accccaggag
    19921 aacgcgatga aaggattctc acacttcatg tccggcgtgg cggtcgcctc gttcggtccg
    19981 tgggccattg aggccgcaca gcaaggcaac ccgatcttct tcatcttggg cggcgcgtgc
    20041 ggtattttgc ccgacaccat tgacttcaag ttctaccgct tcttttacga acatgatgtc
    20101 tacatcaccc ccgacccgct caaccctgac ccgcaatacg tggccaacga gttcgcccgc
    20161 gcggtggcgc tggccgtgga cgagaagcgc tacgtccgcg tcaagctggt gtccgtgcgc
    20221 ctcggcgccg acttctggca gcagtattcg gtcaaaattg acaacgaaaa actggaagtg
    20281 caagtcaagt tcggcccggt ggtcaacacc ggccaggtac ccgtgaaggg cacggaagac
    20341 cgctacccca cggtggccac ggccaagctc aaggccaagg tcatccagac ctatgatgcc
    20401 gcgctgaagg tggacatctt cgacggcccg accatcggct tcaagcccat ggacaacggc
    20461 gacctggatt tggaattcct gccatggcac cgcgagtggt cgcactcgct gacggttggc
    20521 gcgatccttg gcgtgctgct gggggccgtg gcgtggtggg cctccggctg gaccatggct
    20581 tggcaggtgt ttgtgaccat agccgcttgc tacggcgtgc acgtggtgga agaccagctc
    20641 ggccacatgg gctccaacat cttttatccc ctcacgaaga accgcacccc cggcctgcac
    20701 tggatgcact ccggcgatgg cctgcccaac ttcctcgccg tgtggatcag ttgcctgttc
    20761 atcttctgga acctgtatcg cggagttccc aagcccacct atcacttcag ctttatccat
    20821 ctgatgatgg tgggcctggt tattcccggg ctgatcttct ggggcctgcg caagctgctc
    20881 accctcggcc agaacgtcca gatgaaaaaa ggcgacgacg ccgacgacga gtggaacgag
    20941 tccaaggccg cgggctagag catccccccc ccgtttagcc gaacgatttt ggttggagtg
    21001 ttgggcggga atggtcgccg accttccagc ctcttccggc ggggaggtta cgggggtttg
    21061 actttggggg gcgcggcggt atggtttcca atggtattca ccatgcgttt tcagcgcgcc
    21121 attttttgcc gtttatgcct gatggccggc ctgtgggcgg gcggggctac gctgaccgtt
    21181 cacgcaggcg gcggggcctt caacacgctg gtggtggtga acaccaattc cgcggagtcg
    21241 gtggagctgg gcgaatatta cgccagccag cacggcatcc ccacgcacca gatttgttcg
    21301 gtgggcatcg ccacaaacac cgcgacgttg acccccacca attttttcac gttgctgcgc
    21361 gatccaatcc tggcgcacat cgccgcggaa aacctgacgg gccaaattga ttacgttgtg
    21421 ttgtgcggcg acctgcccac acgggtgcgc gacgtggaag gtgtcaccgc ttccctgttt
    21481 tatggcttcc aaaacgcgcc aagcgtctgg gaagttggtg cctgtaacct gccgctctac
    21541 accaccaaca attactaccg tgccgaacgc gcattccgtt ccgtcgccgg ctggaacaac
    21601 accaacggct ttgtggcgtt ccacctgctg ggcaccaacc tcgccaatgc caagctggtg
    21661 gcggatcgcg gcgtcgccgc acagtccacc ttccccgccg gcaccatcta tctctacaat
    21721 ctcggcgacg gcccgcgagg ttggcgggaa caactgtttg gcgatgcgca gttttccttt
    21781 actgcgctgc cgggtttgcc ggcggaatgt ttcatcgccg cgccttgggc cacgctgaac
    21841 aacaaaacca acgtgatggg ctatcatgac ggctgcgctt atctgaccgc cggcgccaaa
    21901 tccaccaaca acacttggct ggctggcgct tacgccgacc acatgacctc ctatggcggg
    21961 cggttgctgg ataccaatca caatcaaaac accgtgttgg actggttcgg cattggcgcc
    22021 acggcttcct acggcaccgt ggccgaaccg tgttcctaca tcgaaaaata tcccgacccc
    22081 ttcctgggct tcctgtatgc ccgcgggttc accattggcg aagcctacgc catggcggtc
    22141 ttcgcgccct accaagggct gttcgcgggc gatccgctgg ctgcgccgta tgccgcgccg
    22201 ccagtcatca ccgtcagcca gcccacgaac aaccaaatcg tgaccggcac cgtgacggtg
    22261 gcgatttcgg ccaccgcgca cgcgcggggc gtgccggcgg ccggctttga tctgtttgtg
    22321 gacaaccggc tgcgcaccaa cttggtcacg ctggccccca cccccggcaa ccaactggcg
    22381 gtggcggttg ccgggcgcac caacagcgtt acggtggccg ccaatgacac gctatttgat
    22441 gtcgtcaacg cgctcgccga tgccgtcaac aacgacgcca cccaaacggt ctatgccacc
    22501 gcttttggcg accggctgga attgattcac cgccaatttg atcacgccgg cgacaacgcc
    22561 gcggtgagcg ccaccgtggc gcaaggcacg gccgactcgc tcacgcttgg cgtgggcctc
    22621 gcggccacca acatcgcccc ctccccttat cacgcccgca aaatggtctg gctggccacg
    22681 cgcactggca gcggcgccaa cgccaacgac aagctcacct gcatcatcac gctgaccaac
    22741 ggcgtggcga tcaccaacac gctggtggcc acgcaaggcg aggcggtcac caacattctg
    22801 gagcgcttgc ggcaggccat caacaccaac tccacactgc aagccaccaa cggcgtgtcc
    22861 tacgcccaca actcacaata ccccgtcaac tacggctacc tcaccgcccg cacgccgggg
    22921 ccggacggct acgggatcgt ggtggattac cgcgtcactg ccgtcagtaa caccaccggc
    22981 ctcgccacca actctgacat gaccacattc ctcgacgaca acgcgcggga cacgcgggcg
    23041 cgggcgtcca ttttgttcca cgtgcggccg accaacggca cactggccgc agaatatgcg
    23101 ctggacacca ccgcgctggc cgacggccac cacacgctgg acatcatcgc gcgcgacggc
    23161 tccgccgtgg ccgcgcagtc gcgccagacg ctcaccattt ttgtggccaa cggcgtcact
    23221 tcgcagggca cgccccactg gtggctggcg cagcacggct ggaccaacga tttcgagaat
    23281 gccgcactgg ccgataccga caacgacggc gtgcccgcgt ggcaggaata tctggcggat
    23341 accgacccca ccgatgccga ttctttcccg tggctggagt ttctcgacgt gctgcaagac
    23401 ccgccggtgc tgacctggcc cgcctccccc gcgcgcgcct accaaatcca ttatgccgat
    23461 gatcttttca acggcccgtg ggtcacgcag caactgctgc aaggcgccag cgaatgggcg
    23521 gacaccaatc cgccccccgc caccaaccgc ttttaccgcc tgttcccgca cctgccctga
    23581 gcgggcaggc gccgcgcgcg actcaatcgt cttcgcccgg ccgcggcccg ccaaacacgt
    23641 agcgcacgta ggccgggtcc atgggcttgg tcaccaacgc caccaccacc gcggtcagca
    23701 cagatagcgg caacgccacc accagcgggt ccaccacggt ccacgtgagg ctccagtgcg
    23761 tcggcacgag gtgatccttg ccgaaccacg ccttgcacag ccctagtccc gccgcagttt
    23821 tgccgttaac aaacaacagc cagaaaccgc tggtcagaaa tcccaccaac aacgaggcca
    23881 ccgccgccgg cttggtcatg cgccgccaga acagtccgaa caccagcatc ggcaggaacg
    23941 atgacgcaca caggccgaag aaaatcgaag tggccagcgc aatgatgttg ccgcgcacat
    24001 atttgcccat caccagcgcc accacaatgc cggccaccac gcccagttgc gtgacccaaa
    24061 tggattgact gccgcgcttt tgcgaatact tggcgaagac atcgcgcccc agcgccgtgc
    24121 ccatggtgtg gaactggctg gacatggtgc tcatggccgc cgaaagcagc gtcagcagaa
    24181 acagaatgcc gaaccagcgc ggcatggcgt ggcgcacgta cgccgggatg atggcatcgg
    24241 cattcccttg cagcgtggtg ccaggccccg ccgagcgcag cgccgccacc aagtgcgggc
    24301 gcaccacatc cagcgcgcca tccgcgccgc gctcgatttc catctggggc gtccacagca
    24361 cgtagttgag cggtgcttcg tcgctttcat ccggcaggcg gaagctgacg aaccgcttgg
    24421 cccgcgcgag ggtttcctca tcggcggtgg ccggcaggac aaccagcttg cccttgccat
    24481 ccgcgccggg gtccagccat gccgcctcct ccacgatccg gcagcggata gcctcctgct
    24541 gcgcgaaata aacgttggag agcgcgccca cagtgaacgc cacgccggtc atgaacaaaa
    24601 taaacacgcc gccagccacc accgcacggt tcaatgcctg cttggatttg accgtcatga
    24661 accgcacgat caattgcggc tgggccagca ccccgatgcc cacgcccatc acgatggtgc
    24721 tcaccagaat ccaccacagg tgatattcca ccgcgccggg catgggagtt tgcggcgcgg
    24781 cgaagccgaa cttgggcatg gccgtccagc cctggtggcc gatcgccttc aggaatggcg
    24841 gcacctggtc gtgcatggcg cccagcgcct ggtgcgccgg ggtcacgccg cccagccggc
    24901 cataggtggc caccagcagg atgatcatcc ccaccagcat gatgccgcct tggagggcat
    24961 cggtcatcat gacgcccttg aggccgccgg caatcacata ggccgccaca atcagcgcaa
    25021 aaatatagag cgcgctatcg tagctgatgt ggaagatggg gccgatgaat tctgcgccgc
    25081 cgaccatcac ggccatggcg tagagcggca tgaagcagaa gataatcagc gcgcacgcca
    25141 cctgcaaaaa gcggctgtcg tagcgcttgg caatcagctc ggggaacgtg tgcgcatcca
    25201 cgtggtggcc catgcggcgc gtcgggttgc ccagaaacac aaacgccacg aaaataccca
    25261 cgccgatatt cagcaccgtc agccacagca gccccatgcc gaagttggcc gccacgccgc
    25321 cgaaccccac aatggccgac gtgctgatga acgtggcccc gtacgacagc gccatcaccg
    25381 ccgggtgcgc gctacgcccc gccaccaaat aatccgtggc attcttggtc tggatatagc
    25441 ccagatagcc cagatagccc gtcagcatca gatacaaaat caccaggaca acggtcagca
    25501 aatccatggc cgcgctcctc ccttacaaat ccacgccggt gggctcgtcg tcgtcgtcat
    25561 ccggcttggc cggctgctcg aggcccttgt tccaatggat gatgccatag cccacgcaca
    25621 acgcggcgca ggccaaactc agcacatacg ccagccaaac tccgggatcc tcaatgccca
    25681 agaccatgtg aacctccttg gccaaaccgg ggcttggcgc caacccggcg cacaccccgc
    25741 tcaaccggac gccatcttgc taactccttc ccgcgcaacg caaccctttt tcccaactat
    25801 tttcactttt tggacaggcg ggtggcgcgc gcggcctgcc ccaccacgcg tggccgaaat
    25861 tggggcttcc cttttttcca cagtgtggaa aaatcggccc cactttttcc acggtgtgga
    25921 aaaataacgc caaagttttt ccattgcgtg gaaaaatcgc gcaaagtttt tccatggcgt
    25981 ggaaaaatcc gcgtggattt tccatggtgt ggaaaacttt tttccacagt gtggaaaaaa
    26041 agagcgtaat ttttccacgg cgtggaaaat cccgcccgcg cgggttgaac ttttttccac
    26101 agtgtggaaa acggagatga gccaggagcc tttgccatga tgacagccgc ccagattcgc
    26161 caatcgttcc tcgacttttt tgcgtcgaag gaccacgaga ttgtgccgtc gtcgcccgtg
    26221 gtgctgccga cggaccccac gctgctgttt gccaacgcgg gcatgaacca gttcaaggag
    26281 attttcctcg gcgcgcgcaa gtcgtcgtgg aagcgggtgg ccaacacgca aaagtgcatc
    26341 cgcgtgtccg gcaagcacaa cgacttggag gaagtgggcc gcgacaccta tcaccacacc
    26401 ttttttgaga tgctcggcaa ctggtcgttt ggcgactact acaagcgcga ggccatcacg
    26461 tgggcgtggg aactgctgac tgtcgtctgg aagctgccca aaaaccggct ctgggccacg
    26521 gtgttcacca ccgatgacga ggccgccgat atttggcgca cctgcaccga tattgatccc
    26581 agccacatcc tgcgcttcgg cgaaaaggac aacttctggg aaatgggcga gaccggcccc
    26641 tgcggcccct gctcggaaat ccattttgac ctgacccccg acggctgcac gccggacatg
    26701 atcaacgccg gctcgccgct ggtgatcgaa atctggaaca acgttttcat gcagtacaac
    26761 cgcaagtccg acggctcgct cgacgagctg ccggccaagt gcgtggatac cggcatgggt
    26821 ttcgagcgcg tggtggcggt catccagggc aagacctcca actacgacac ggatgtgttc
    26881 atgccgctga tcgaggccgt ggccgcgctt tccggcaagg cctacaccga aggcgaaaac
    26941 gccgtggcca tgcgcgtgat tgccgaccac ctgcgcgcgc tgtcctttgc catcgccgat
    27001 ggcgtcatgc ccggcaacga gggccgcggc tacgtgctgc gccggctgct gcgccgcgcc
    27061 gcgcgctacg gccgcaacct cggcctcacc aagccgttcc tgtgcgagct gttccccgtg
    27121 ctggaaaaac aaatgggcgg cttcttcccc gagctgaccg cgcgccgcaa cgagatcgtc
    27181 cgcgcgctgc gcgccgagga ggaatccttt gccgccacgc tggaccgcgg cattgcgcac
    27241 ttcaacgagg taatcgaaaa actcggctcc gctggcaaca agctgttccc cggctccgag
    27301 gcgttcaagc tctacgacac ctacggcttc ccgctcgacc tcaccgtgtt gatggcgcag
    27361 gaaaagggct tccaaattga tgcgcccgcc ttcgaacgct tcatggccga gcagcgcgcc
    27421 cgcgcccgcg ctgcgcgcaa ggacaacgcc gccgccgaca acgacctgat tgcccacttc
    27481 gtggagcaag ggctgcaaac cacctttacc ggttatactt cgctgaccac cggcacctcg
    27541 ctggtggcgc tgctggccga cgggcagatt gtggatacgc tggccaccgg ccaaaccgcc
    27601 gacctgctgc tggcgcaaac cccgttctat gccgaggccg gcggccaagt cggcgacacg
    27661 ggcgtgatca ccacctgcca gggcgcgcgg tttgaagtca ccgacaccca aaagcccgcg
    27721 cagggcatca tcctgcaccg cggcaagctg gtggccggca ccctcgccaa gggcgcagcc
    27781 gtggtggcca cggtggatga ggaacgtcgc gcggccatcc gccgcaacca caccgccacc
    27841 cacctgctca acgccgcgct gcgcaaggtg ctcgccaacg attcgctcaa gcaggccggc
    27901 tcctatgtgg atgccgagcg gctgcgcttc gacttcacct ctttcgaggc gctgaccccg
    27961 gcgcagttgg cagccatcga gcggcaggtc acggagttcg tcatcgccaa ccgcgcagtc
    28021 aaaacctacg aaatcccgct gaaggatgtg cccggctccg gaatcattgc cgtgttcgat
    28081 gaaaaatatg gcgacatcgt gcgcgtggtg gaaaccgaag gcatctcgcg cgagctgtgc
    28141 ggcggcaccc acgtcgaacg cagcggcgac attggcccct tccgtattct ggcggagacc
    28201 tccattgccg ccggcatccg gcgcatcgag gcgcaggccg gcctgcccgc ctgcgaccat
    28261 accgcgcacg agcacggcat cctgtccgat ttggcacacc gcctctccgt ggcccccgcc
    28321 gatctgccgc cgcgtctcga agccctgcag cagcacgccc accagttgga aaaagaattg
    28381 aaggccctcg cgcagaaaaa cgcgctggaa aaaggcgcgg cgctggccgc caagtttgcg
    28441 cccatcggcg gcattccggt gctgattgaa agtgtcggcg aactggaagc tgagccgctg
    28501 cgcgcgctgg tggaaagcct gcgcaagcaa acgccggaga gcgtgatcct gctggcagcc
    28561 accgccgagg acaaggtgac gttcatcctc aacgcgggcc ccgcggcgca ggccaagggt
    28621 gtccacgcgg gcaagctggt gggcgccatc gccaagctga ccggcggcgg cggcggcggc
    28681 aagccggaca aggcgcaggc cggcggcaag gacgcggcga agctgcccgc ggccctcgcc
    28741 cacgcccgcg aattgatcgc ccaagcgctg ggctgaacgc ccgcccccgc cgtacccccg
    28801 cccccggcgc gaaaagtgtt tgcgcgacgg cgcaggtggc tataatgagc ccgatcattt
    28861 tcaaggaagg accgcgcaca tgcccacacc cattattgcc gttgccaacc aaaaaggcgg
    28921 cgtaggcaag accacgacgg ccgtcaatct ggccgcctgc ctcgcggaac ggcgcaagaa
    28981 aatcctgctg gtggatttgg acccgcaagc caacgccacc agcggcctcg gcctggccaa
    29041 aaccgagggc gcgagcatct acgacagcct gctgggcgac cgcccgctgc aggagatcat
    29101 tcagccgtcc gccgattccc ggctggatgt ggtggcgtcc gaggtggatt tggccggcgc
    29161 ggaaatcgaa gtggcccgcg cggagcacca cctgctgcgg ctggccatgg cgctggcccc
    29221 cctggccgcg cagaatcact acgactttat ttttgtggat tgcccgccct cactgggcat
    29281 cctgacgatg aacgcgctgc acgcggcgga tggactgctc atccccatgc agtgcgaata
    29341 ttacgcgctg gaaggcatca gcgtgatttt gaacttggtc cacaagctgc acgatagcgg
    29401 cgcggcgcca cggttggccg tcgagggcat cgtgatgacg atgtatgaca tgcgcaccaa
    29461 cctctcgcag caggtggttg gcgaaatccg cacccacttc ccggacaaaa tttacgactc
    29521 gctcatcccg cgctcggtgc ggctggcgga agcgccgtcg cacgggctgc ccatcattgc
    29581 ctatgacccc aactgcacgg gcgcggcggc ctatcggcaa ctggcccgcg aattcctgaa
    29641 gcgccgcaaa actgccgtgg aacccgccgg cgcggacgcc ccccccgccc ccgccccgga
    29701 cgccccgccg gcgtaatgga ggccgccccc accatcatgg cggccacggg tcatctgcgg
    29761 gtgtgcgtgc tggcgtcggg cagcagcggc aactgcatct atgtggaaag ccccaccacc
    29821 gcggtgctga ttgatgccgg cctctccgca cgcgaaaccc tgcgccgcct caccgccatc
    29881 gggcgcgagc cggcgcggat tcaagccatt tgcatcacgc acgatcacag cgaccacgtg
    29941 gctggcgtgc cccgcctgca caaactgcac ggctgggaac tctacgccaa cggggacacc
    30001 gcccgcgccg tggatcccaa acagcaattg ccgtggcggc tgttcaccgc cggctcgcgc
    30061 ttccaaatcg gcgatattga gctgctgccg ttctcactgc cgcacgatgc ctacgacccc
    30121 gtgggctttg tgctgaccca cggccgcagc cggctgggca tcgccaccga tctcggcatg
    30181 gccacgcagc tcgcccgcca ccatctgcgc ggctgccacc tgctggtgct ggaggccaac
    30241 cacgatgaag tgatggtgaa aaactcgttc cgcccgtggg aactcaagcg gcgcattttg
    30301 agccggcagg ggcatctctc caacgaaacc gccgccgagc tgctggtgga cgtggccggt
    30361 gacaccttgc agcaggtgtt cctggcgcac ttgagcagcg agtgcaacaa cgttgacctg
    30421 gccctgcata ccgtgcgcac gcgtctgacc gccgccggct tcggccacgt gcacgtgcgt
    30481 cccacccacc ccgaccggcc cagtgaattt ggcgaactct tttttgacaa ccccgcaggc
    30541 tgatatgcaa accatccaag acctcctttc cgaacaagtc cgcgcggcgt ttgccgcggc
    30601 attccccggc gcggacctcg ccggtctgcc gctggccgcc acccccgccg gtgatgaagc
    30661 ttttggcgat taccaatcca acggcgcaat ggccgccgcc aaggtgctgc gccaaccgcc
    30721 gcgccaagta gccgaacagg tggtccgcca cctaccgacc ggcgacctgc tggccaaggt
    30781 ggagatcgcc ggccccggct tcatcaacat gacgctggcg gatgacgcac tggcgcggca
    30841 cgtggccgcc atgcaggatg atccgcacgt gggcctgccg caatccggcg ccggccggcg
    30901 gctcattctg gactattccg cccccaacgt cgccaagccg atgcacattg cgcacatccg
    30961 ctccacggtc attggcgatg cactcaaacg catctaccgc gcgcttggct acgaggtgct
    31021 cggcgacaac catttgggcg actggggtac gcagttcggc attctgatca tgggctaccg
    31081 ccactttttg gacaaagctg cactggcggc ctcgccggtg gatgagttgc aacgggtgta
    31141 tgtgctctcg caccagcgtt gcgaagagaa ccccgcgtgg caagaccaag cgcgcgcgga
    31201 attggtcaag ctacaagcgg gcgacccgga aaaccgcgcg ctgtggcaaa ccttcattga
    31261 tttgtcgctg gaggaattca accggattta cgcgcggctg ggcgtgtcgt ttgagttgat
    31321 ccgcggcgaa tcctattaca acgatgccct ccccggtgtg gtgcaaaagc tgcaagacct
    31381 tggtctcgtg cgcgagagcg aaggcgcgct ggtggcattt ctggacgacg aaaaattgcc
    31441 gccgtgcatc gtgcgcaaat ccgacggcgg ctacaactac gccacgacgg acctcgccac
    31501 cgtgttctcc cgcgagcagg aattccagcc cgccgccatt ttgtatgtga cggatgaacg
    31561 ccagcagttg cacttccggc agttttttgc catcgcccgc aaggcgggcg tgaccacgcc
    31621 gctgcggcat atctggttcg ggctgatgcg attgccggag ggtctgctct ccacccgcaa
    31681 aggcactgcc atcaagctgg acctgctgct ggacgaagcc gaaaagcgtg cgctggaact
    31741 ggtgcgacag gcctcgcccg acatgcccga agcccaacag caggccgtgg cgcgcgcggt
    31801 gggcatcggc gcggtgaaat atgcggacct ctcgcaaaac ccgcagtccg ccgtcagctt
    31861 cacgtgggaa aaagcgctgg ccatggacgg caattccgcg ccttatttgc aatatgccta
    31921 cgcgcgcatc gcctcggtgc tggacaagca cgcggagcgc tttccgggcc acgactggtg
    31981 tgccgcaccg ctggtgttgg actcgcccca cgcccgtcgg ctggcgctgc ggctggcgcg
    32041 atattccggt gcggtgtgcg ccgctgccgc gcaggaaaaa ccgtcgctgc tggcggatgc
    32101 actctacgat ttgtcgcagg cgtattcctc gttctaccag aacgtgccgt tcctcaaagc
    32161 ggaggctggc ctgcgcgaaa gccgcgtgcg gctctgcgca ctgaccgcca aggtgctgcg
    32221 tcacggcttg ggcttgctgg gcatcgagac gcccgaccgg atttgaatcg cggtagggcg
    32281 ggaacggttg aggaacgaaa ctttcccgcc gagaaagcgg ctggaagatc tgcaaccgtt
    32341 ctagccctac aaaccaaacc cgaacacaaa ccaaacccgg ctgcttttgt agggcgggaa
    32401 tggttgagga acgaaacctt cccgccgaga aagcggctgg aagatctgcg accgttccag
    32461 ctctacaaat caaacccgga cacaaaccaa acccggctgc ttttgtaggg cgggaatggt
    32521 tgaggaacga aaccttcccg ccggaaaagg ctggaaggtc tgcgaccatt ccagccctac
    32581 aaaccaaacg gcatccgttg ccggatgccg tcaggggaaa accgctggtt gggcggctta
    32641 ctttttgtcg tccttcttgg acgttttctt ggaatccttt ttggcttcct tcttgtcgtc
    32701 cttcttgggc tccttggcat cctttttgcc cttggccttg gcttccggcg gcacaaagtc
    32761 cacccactcc agcaggcaca tttcgctgct gtcgctcatg cgcttgccga gcttgatgac
    32821 gcgggtatag ccgcccttgc gctcagccat ggccggcgca acggcgctga acagttcctg
    32881 gacggctttt tcgaggcgca gggtggcgat cgcctgccgg cgagccgcca gcgtgccctt
    32941 cttgcccagc gtgaccatct tgtcggccag acgcttggca gcgcgggcct tgggcagggt
    33001 ggtcttgatg cgcttggcga ggatcagatt gcagaccagg ctggccatca gcgccgagcg
    33061 ctgcgccgtg gtccggccaa gttttacggt gttcttgcga tgacgcatgg cgagtctcct
    33121 gtctctctta ttcctcggcg ggctgggtca tgttcagcag ctcggcatcg aaggtcatgc
    33181 ccaaggacag acccagttcg gccagcttct gtttgatttc gttcagcgac ttcttgccga
    33241 agttgcggta cttgagcatt tcgttctcgg tcttctgcg
//
LOCUS       DAOPNP010000010        32402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1804_length_32402_cov_9.095434, whole genome shotgun sequence.
ACCESSION   DAOPNP010000010 DAOPNP010000000
VERSION     DAOPNP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 32402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32402
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1804_length_32402_cov_9.095434"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(524..784)
                     /locus_tag="PKI64_01915"
     CDS             complement(524..784)
                     /locus_tag="PKI64_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOD99795.1"
                     /translation="MAKYEVLLRQSVLKQDLLALPKADVQKIVKAIRGLGEEPRPRGV
                     QKLTGQEYYRIRQGRYRILYSIQDEEKTVWVVKVGHRREVYR"
     gene            complement(771..1007)
                     /locus_tag="PKI64_01920"
     CDS             complement(771..1007)
                     /locus_tag="PKI64_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOD99796.1"
                     /translation="MPTLPPKRATVYLDPHLHKALRIKSAETEYSISALVNEAVAISL
                     AEDAEDIAAFKERAREPLISFEDALKDLKRHGKI"
     gene            complement(1435..2148)
                     /locus_tag="PKI64_01925"
     CDS             complement(1435..2148)
                     /locus_tag="PKI64_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD99797.1"
                     /translation="MRILIIEDAEIDRLNLRTLLEDHGDVDIVGEAPTLDAAVELLDE
                     EQPDAVFLDIHLGRQKGFKALELAERRPLVVITTSHPHYAIKGYEVGAVDYLLKPVSE
                     EPLARALTRLRERLAAQEQGGGLDPDDMQSFKVGDDRHMIRVRELQAIVGERVYTRVL
                     VRDGREFLHNRPLREWRFMLPRKLFRALDRSTIVNVTEVKMVRLGDDGVSRLIFQSSG
                     HRLALGAVALKKLRTHINV"
     gene            complement(2164..5415)
                     /locus_tag="PKI64_01930"
     CDS             complement(2164..5415)
                     /locus_tag="PKI64_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD99798.1"
                     /translation="MSKWRQMVLVLLIAGLARVVTGQEPAVLTPETTSLKLRQQTAVW
                     RDGAGSATLAEAVAAWKASKFVPLGEQAAVQRFTQDAVWVHFVLQTQMPERATWYLRR
                     PDPSAEEVDWYVMRAGGDVTHVADGIRYARCGPGTVNRHNPVISVELAPAETVEVYVR
                     LRSDNQMDVRFSVFSPAAYLRWAEMEDAFVYFCLGAFAVLGALGILQAVFTRSWGFLT
                     YAASAVMVWLVLVGTSGHWRHWGWPGWRYVMYNGHLVFNGLAILALLAYLRWFFSLAK
                     ESPRVDRGLRWTMAGVVVLVVWLAAGPFPLRQAVMDVVSLVAGAGSLAIGMMAWKRGN
                     RTARFYVLAWWIFWVLVVLEFGQDWHWWPTVAPTNMLPTVGLLVGFFLFQAAMADRLR
                     QEQVNRALAQAQADALLREHTAELEQQVAERTAEVSAAMRELAEANLNLQENQQRLEA
                     MLSTIPDLIFRVDAAGVILEFHAATPNILYASPAMFLGKNVREALPPEAADVIVEALA
                     EAVAHGSHRGSVYSLPLSTGLGWFELAIAALPKDVGRTAEYLVVVRDITAQRLAQDAL
                     LAAEQKFRLLTENMKDVVWILDAETLRFLYVSPSVEKLRGYTPDEVMAEPLDAAMTPE
                     QAKHIRAETQRLVQILRSSGQPFSSFVTEEVLQPRKDGTFVHTEAIVRYWQNPNNGRV
                     EIHGVTRDISDRKRAEEALRESEQKYRFLTETMKDVVWILDTETFRYTYVSPAVINPR
                     GYTPEEIMAAPFEDSVIPQQVTRFRDTVNAVCREMREQGREFSGFFTEEATQPCKDGS
                     VIYTEVIARFWRNPKTDRIELHGVTRDITERKQAEEALRAKHEELLIAKEAAEKANYA
                     KGLFLANMSHEIRTPLSALVGLSQAMVRLGERRGLPEDFLRMLEQIRSGGRHLNLLLT
                     NVLDISATEGGQPRVHWRTVNLAEWAQQMRDILQPIAEAHQVELRWESGALAGKTRQS
                     DPVRLAQIVINLVHNAVKFTPAGRAVTVQFTHTPDLFACDVLDEGAGLPAEVGSTFEA
                     FTTGSPAVADLEHGAGLGLHVVQTNVRLLGGWVQAQNRPTGGAHFRVEWAGPS"
     gene            complement(5478..5879)
                     /locus_tag="PKI64_01935"
     CDS             complement(5478..5879)
                     /locus_tag="PKI64_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99799.1"
                     /translation="MYPIRGAMRSNTKNKNTIKQGPSPLAPVESLIRKLEKVLGKLRV
                     GSGAEKRVQEQLNALQYAQEWMTGAGSVGRQITNSERQEMMRVIMAMIRVTQKMKNKF
                     AVGTAQHTLLRNRLVGLRAAHAALRESAPKE"
     gene            5988..8519
                     /gene="hrpB"
                     /locus_tag="PKI64_01940"
     CDS             5988..8519
                     /gene="hrpB"
                     /locus_tag="PKI64_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOD99800.1"
                     /translation="MLPIYAIREELTATLKRSRRIILTAPTGSGKSTQVPQMLLDSGA
                     LGTGKVVVLQPRRLAARLLAKRVAEERRVPLGDEVGYQVRLESVCGPKTRIHFETEGI
                     LLRQMLSQPTLPGVAAILFDEFHERHVYGDVTLAQALRLQQTTRPDLLLMVMSATLDA
                     RLLQPYLEPCAHLHSDGRTFPVTIEHLAKPLHPDHDPPWDAAATAYERAARAGEAPGD
                     TLIFMPGAYEIQRTIEALRHTPAAKGALLLPLHGELPPHEQDAAVARHDCKKIVVATN
                     VAETSLTIDGIRLVIDSGLARVARFDPWRGINTLLIEHISRASADQRAGRAGRTAPGR
                     AIRLWTEQEHAGRAAHDVPEIKRVDPAEILLTLKALGVADVAAFPWLEPPEPRAQDRA
                     LTLLHDLGALDATGAPTAVGLRMLNFPVHPRYARMLLAAAERGAVRPVALMAAIAQSR
                     PLLVRNAGRQAVEQRRDILGEAGGSDLFLLMRAWNYARHNQFDLQRCRKLGIHAAAAR
                     QVGPVWDYFLRIAAAQQLPLESTPPEDADVAKCILLGFSDQVARRLDRGTLRCELVHK
                     RRGELARESIADEAPLLVANEINEIGRSDGEISVLLTQATAIREEWLRELYPDDFSAR
                     ITVQWDAAAKRVVARQEVVFRDLALDSRPSEPPPAEEAAQLLAKAVLSGECALKQWDR
                     TVDQWIARVNLLAAVCPDWQIPAYDEASRELLIQQICHGAVSHKEIKDRPVLSAVKSL
                     LSPAQIALVEKHMPERVTLANGRAVKVQYTDTAEPFISARIQELFGVESLPTLASGRI
                     PLVIHILAPSQRPVQITKDLPGFWRDHYPRIKQELRRKYPKHEWR"
     assembly_gap    8569..8668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8681..9352)
                     /locus_tag="PKI64_01945"
     CDS             complement(8681..9352)
                     /locus_tag="PKI64_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOD99801.1"
                     /translation="MKKIVHLGACLLLVFVAAYAQQMTDIHATTDPVFNQEPQASENL
                     STQEQDADTNRSAQVEALLQRITLRREFKAGHITEEEFLRQKASLDEIIHEPIAYSND
                     NPPEVASHMAEDPDLQARVNAFQKKLILRGRMEMRAITQEEYDREAAPLDDIIQYPGI
                     LTNRVITEQVMSPDLREQVDAFKRYITLRRKYQRGQVSAEEYDRQFKFLDEIMNRSVR
                     WTIIE"
     gene            9604..10875
                     /locus_tag="PKI64_01950"
     CDS             9604..10875
                     /locus_tag="PKI64_01950"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOD99802.1"
                     /translation="MTTPEKLFSELLGLGDAWVVERATFDEAKNALELHVKETPELWV
                     NERSPHDGSTGVVCHDHVRGLRWRHLNIFSKEAEIVCDLPRGRCPQCGKVWRVTPPWE
                     GRAKHLTKEFEAFALTLMREMPVKKAAEILGEQDTRMWRVVQAYVEEAHAAIDMSDVT
                     CVGVDEMTRAKGHRYLTVFADLVKKAVLFATPGKDSETWAEFVQALAIHNGHPHALTH
                     VSMDMSKAYAKGVRDYCRNAQIIYDKFHVIAHANQAVDLVRRAEAQSGSATARAVLVK
                     SRWLWLKNPENLTRKQRAPLRQIDQNMLQTGRAYQMRLVLQEIYALDSAAAAAERFGE
                     WCRWVRREARKASRRLLHHMARVADLIESHIAGILAHWEQGITNAFMEGLNSVFSAVR
                     RKARGYRSIRNMVAMLYFVAGKLPSPAILSH"
     gene            complement(10827..11438)
                     /locus_tag="PKI64_01955"
     CDS             complement(10827..11438)
                     /locus_tag="PKI64_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99803.1"
                     /translation="MNTTGGNRTNCRNFLNRLILILGLFWLHSSRAEPVARPAESWEE
                     YDLIAKVVVRSVDASQEVKTYDTAEAQMFAHPVEVEVVAPLRMPGTGVTWTVLAPGYG
                     IYRGKRLDVYYWTNGVYSTTNVLALACRFARDKATLAVDWVLTDEEWETYRRQKETGV
                     VERPLSQEDYEIQQRLDAANKLEVPRYFQWLSMAGEGSFPATK"
     gene            complement(11392..12015)
                     /locus_tag="PKI64_01960"
     CDS             complement(11392..12015)
                     /locus_tag="PKI64_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99804.1"
                     /translation="MKIINIIVLSIMTGALSGYGEMVAAPADLWTKYDLIAKVTILTV
                     HTNQEVLYFDSPRGKSYGYPMEVEVTEALRMPPEMEPGRIQLLAPVYSIHGDRRLEFD
                     FYSKGEYSAGNQPAVACSYQRRNGTWLVSEWVLSEAQWAEYRDQKERGVSSVDSPRSE
                     SRDMVENARELIRQWRGGEISMEEYNERMTPIREYYRWQSDKLQELP"
     gene            complement(12025..13206)
                     /locus_tag="PKI64_01965"
     CDS             complement(12025..13206)
                     /locus_tag="PKI64_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99805.1"
                     /translation="MSKLSQWIPVALVAALAWTGALKAEGETFTYEGDPDRPFPDAWG
                     DPDVDCSDPHDPGESDCWKCDSDSPPKPVPKFEWILTRECPTFSMSNTVQSNCYCVLE
                     GGEMTDSITAGYTAEDGRKYYEPSIEGCGGEPPEEEAITQTIEWNWTTAGLTTEPASG
                     QGETASFKYTVPKGPFSIDVFFSAKATPSDTDCAEITAGPEVVGQITGAGYEKYQPPE
                     PEKKNPTPQMVAWPLYHGDQKAWGKIQYRIKNCLTPAPVCEGSDCSLCRIEGYFYLID
                     VGILVVTNMVVTANNCDLDVGSSRARTQTRITETIDHEQLHLALLERFVAEWNAKIPD
                     LSLADSCDYAQIILDEFLTEYEAAKIKFEDNPHCPEFDGAATFKFDACNEVYSGKINC
                     N"
     gene            complement(13219..15744)
                     /locus_tag="PKI64_01970"
     CDS             complement(13219..15744)
                     /locus_tag="PKI64_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOD99806.1"
                     /translation="MHAQPIEYQETQIAFSPKGDALTLILGALQRARRQVDVAMAFLT
                     HDDLINALCLVAQRPGVTVRFITDDEMAKPAQRPILERMALAGIETYVVTVQSGKMHL
                     KAMVVDEDLVISGTANWTRQAFEANIEDTVSMRSAPLAQKYREQMNRLIAEATPIVAY
                     GGEDPRQLAKRYRNMRWPVPAAPGGNHRGLAGPRSRTFRPSRMDVLSRTGEGNMDAWF
                     TFEPGVGEALAASIRSATNRIDVGMYLLNQPELVQALADQAQNPQVRVRALFDIQMRE
                     GGFMGILHDLAEAGCEVGIYGGDRHSLHLKTLVVDDRQVWTGSANWTKAAMGMNIEDI
                     LCFHSSDMAAYYRTFLGQVAVHCMSFDPPVAAAGEVPAEGCGRLPPTGPRTNFTALAR
                     PPFEAFEFEAQARYLPDEQYLPALLDVIRTADQSLLMLMYAFPAPETAGEAQAALVRE
                     MSCAAARGVYVYLGLYTPPGGKDRLGDMHSAWAERLRATGVDVRLSIPNGAQHDKLVV
                     ADLRRVLIGSHNWSEGALSGKRVYEASVLLDLPQPDPRWADYVLGRRMVDDMRTRQQW
                     EAEIDLLRRLQSLPSHERLEQTALLEAEAVPAPSAASWTRVREEFMTESGDRILLRDG
                     RLLAGQWLVGQPLRHQWRFPDGQIRWIDKSAVQLLWRASASTPPARRPADANSGSTVK
                     TAFLPQTAFGADLLEGVKKARKSILIATYNMSEGLYGPLGEFYDVLRQKSQEGIEVAF
                     VTEFGPGTSAFLKRAVLNFASTLATGNVQVRFMQAYKVMHKKLVVVDGETVWLGSANL
                     TTAGLSISSEANVRIQDTTVAGAAEQDVRSLFEQAKPLSELKY"
     gene            complement(16139..17983)
                     /gene="dnaG"
                     /locus_tag="PKI64_01975"
     CDS             complement(16139..17983)
                     /gene="dnaG"
                     /locus_tag="PKI64_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD99807.1"
                     /translation="MAAMVSKDTIDQIRQALDIADVIGSYIQLKRTGHTAKALCPFHK
                     EKTPSFHVNPARQAFHCFGCGAGGDVFKFVMLYDHVDFPTAVRMLASRAGIPLQLEET
                     HGGAREGPPKDELFAANEAAAKRYLKELIQSADAAPARDYLNGRELGLDVWKEWGIGW
                     APEGYGFLADKAGPRAAIKREAMEAAGLLVSNESGHLYDRFRGRVMFTIRDELGRAVG
                     FSGRILQSDDKNVGKYVNTPETPVFRKSRILFGLDKAKRAILDARQALLVEGQIDCIR
                     CHLGGFTTAVAAQGTAFTSEHAAVLKRYADHILIVLDADEAGQKAALRTAELLIVEGL
                     AVSLAALPAGEDPDSLIRGQGSAAFQALLDKAQTAMGFLLDVLRAKEDVRTPEGLLRA
                     TRAAVDLATRAPSAVQAEQMLREAATGLGIGYDALRRDLRAAVRRKFRPASAAAESSA
                     TPTAAPPVDEVELAMLLGAQGSPEVAGLVRQWIPYPLLTDPTCRAVIQALAEEEPDLM
                     AAVADAGEAAQVLAAQVVNAPAKIRGGDADFGVEKAAQDLIVRIWQRHLDRRRQELSQ
                     RLRTLTGAEHEQAIRELAELLTDQGKLKLGWAQAQSVLTRHLDRVCGI"
     gene            complement(17990..18607)
                     /locus_tag="PKI64_01980"
     CDS             complement(17990..18607)
                     /locus_tag="PKI64_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOD99808.1"
                     /translation="MDWATWNWVDWLFVAVLLYGAVMGLIRGLSHELATLISIVVAVL
                     VTRLGYEPLAAWACARWGWNEEITRLVAVVALALATLLGMRLLRIALGSLMTFAFKGW
                     VERLGGLLVGAVRQGVIFLVILLAASFVPSAWVQREVMYNSATGRTTLPFLVEKYNEV
                     AARARMIQADIPGGVEEPHAILPPLPEPDPVVGLDEYAPLYPVEE"
     gene            complement(18623..19135)
                     /locus_tag="PKI64_01985"
     CDS             complement(18623..19135)
                     /locus_tag="PKI64_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOD99809.1"
                     /translation="MKFSEIDWAQWRPRETATLMFIRMDGMVLLIRKLQGMGAGLINA
                     PGGRVDPGETPRQGAIREAQEELKVTPYEPTEAGVLDFEFTNGYSLRCHVFTAATYDG
                     IPTETPEAIPLWIDEREMPYNQMWADDRVWMPLVLANRKFHGRFLFEDEQMLGCEIDV
                     APRAGVELAS"
     gene            complement(19220..19786)
                     /gene="yeiP"
                     /locus_tag="PKI64_01990"
     CDS             complement(19220..19786)
                     /gene="yeiP"
                     /locus_tag="PKI64_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HOD99810.1"
                     /translation="MIKACDLTKNSVVELNGDPHVVEQLRVQTPSARGAATLYKIRFR
                     NVRTRAKLDQTFRGDDPVKETDFDTRQVSFSYREGDRFTFMDVDDYTQFELMADEIED
                     AVPFLVDGMEGIKALTQEGKVLCIQLPDAVELTIVECAPAMRGASVTARTKTAKLETG
                     LELQVPEYMAQGERVRVDTRTGGFIQRA"
     gene            19903..>20249
                     /locus_tag="PKI64_01995"
     CDS             19903..>20249
                     /locus_tag="PKI64_01995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD99811.1"
                     /translation="MIAERSWRFPNRMPALTKVVREVSAWMDELPFSNRAKYSATLTV
                     EEMGSNIVKYAYDDSDEHEIHLHMVANQDVVEITFEDDGHPFDPTQQPPPDMEKIVAA
                     PKSGGLGIELVRR"
     assembly_gap    20250..20349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20350..21495
                     /locus_tag="PKI64_02000"
     CDS             <20350..21495
                     /locus_tag="PKI64_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOD99812.1"
                     /translation="ATSVTQAREAFSALGHLTLVPDREITRAHLVAADALIVRSKTTV
                     NAELLADTPVSFVATATAGADHFDIPWLNDADICWTASPGCNANAVAEYVTTVLTLLA
                     RRRAALLPGRTLGIIGCGHVGSLVAEKAGLLGLRVLRNDPPLALQTNSADYLPLAEVL
                     PHADIVTLHVPLENGGPFPTRRLADCRFFEILKPGAWFINSARGEVVDSDALQCALDH
                     HLIAACALDVWDGEPHLADALKNAVDFLTPHIAGYTLEGLLNGTLHCYRHLCQFLEVE
                     PTWTPDPRLLPPAPFIEIDAAHRADDDVLAELLATACPIPCDTRAWRTDMATPDPAEL
                     RRRFDRFRKNYTSRREFSAIRVHLAHATPALLETVAALGFQVCAHSP"
     assembly_gap    21744..21843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21844..22106)
                     /locus_tag="PKI64_02005"
     CDS             complement(<21844..22106)
                     /locus_tag="PKI64_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99813.1"
                     /translation="MAAQKPTDIFSALAHCARPLADMSPARVVRHAPSSENLPVIFAR
                     QPRQNPQEILRVHVLRTADNRIMSCGLLAASGSCFPVFPVFLLG"
     gene            22161..23099
                     /locus_tag="PKI64_02010"
     CDS             22161..23099
                     /locus_tag="PKI64_02010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD99814.1"
                     /translation="MTTDATPITFTVTARAPRLDVWLAAAHPAITRSRWQQLIKDGHI
                     TLNGTPLCKPNTSLAPGDTLLFTPPAPTPSPLAPADIPLAILYEDDDLLVINKPAGLV
                     VHPAPGHPGNTLVNALLHHCAHLQGIGGELRPGIVHRLDKDTTGVLVVAQNEAAHAAL
                     VAQFAGRTTRKEYLALVWGIPAKPRGEINQPIGRHPVHRKKMAVTAKGRAALTRYEVM
                     GTGPRAALLRLEIETGRTHQIRVHLAHLGHPVVGDATYGRARSLPPDAPPITRQMLHA
                     HQLHITHPRTHAPLVFTAPPPADFLALQHHLIPPPL"
     gene            23429..24664
                     /locus_tag="PKI64_02015"
     CDS             23429..24664
                     /locus_tag="PKI64_02015"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOD99815.1"
                     /translation="MKIVLAYSGGLDTSVILRWLKENYQAQIIAFAADVGQEEELKGL
                     RKKALATGASKIYIDDLQEEFARDFIFPMMRANAIYEGQYFLGTSIARPLIAKRMVEI
                     ARQEGATAIAHGATGKGNDQVRFELTAAALAPDLQVIAPWRNAAYREQFPGRSEMIAY
                     CAKHGINVEASAKKPYSMDRNLLHISYEAGILEDPWFDAFAPQNRGMFKLTVAPEDAP
                     DRAEIVELEFVRGNCVAVNGKKLSPLGVMRALNKLAGRHGVGRVDMVENRYVGMKSRG
                     VYETPGGTVLQFAHRQMESLTMDRETMHLRDGLIPRYAAMVYNGFWFAPERVALQALV
                     EETQRNVTGTVRVRLYKGNLYVAGRKSAVSLYNPQMATMDADPTKAYNQDDATGFIRL
                     NALRLKVAAKVQGKRKKSF"
     gene            24754..25944
                     /locus_tag="PKI64_02020"
     CDS             24754..25944
                     /locus_tag="PKI64_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HOD99816.1"
                     /translation="MKILIADKMSKNAITALEKLGHTVISNPDLKAEDLPGAIGDAEV
                     LIVRSTKVIAATIEAGKELSLIIRAGAGVNTIDLAKASEFGIHVTNCPGRNTEAVAEL
                     AIGLLIAADRRIADATADLRGGKWRKKEYGKAAGLKGRTLGILGVGSIGKAVVQRAKA
                     LEMNVMAWSRSLTPEKAKELGVGFCATPADVAKVADAISLHLAAKPETKKMINAEFIA
                     LLKPGAIIVNTARGEIVDWTALKDALAAKKIRVASDVFDAEPAGGEEPFADTSLAAAM
                     AACTPHIGASTDQASEAIAEEAVRIVKVFQETGNPPNVVNIRKKTSAVINLVVRHFNK
                     VGVMAHVLDCLRGAQINVEEMENLIFEGGLAACCTLKLDKKPEAALVEKINAGEHVIS
                     AALK"
     gene            25969..27489
                     /locus_tag="PKI64_02025"
     CDS             25969..27489
                     /locus_tag="PKI64_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99817.1"
                     /translation="MKRILLLLACAGWLMAGPVQAELSDPGQIQTEAYIQLVQADQSL
                     DAGRLDEALRQYQAARDYYVRLAREFPGWEPRVIQYRKTYCDNQIADIQRQQAGGSPR
                     EEWPELEPLPGEAPVTTPPAAAVVPEEPAPVADRSVEINYLKSRIASLEEELRTRDSQ
                     QAEYDTLAAQYKQCRQELANANQQVQDEKNAGQSALAALQAEADKKDEQLRDLRKELK
                     SKKQLDQALNDLEAKVNELQAQNQRLDDEIQALNRELDDAEIRADQAELKLQQAEASL
                     KAANKALEKAEKAAQARVQKDEPAPAKTQEAAAAKPSRKPAVADVPMPPVAPEKAPPA
                     EQPAPPALPPPVKVTSTAAPREVPAGMNAADFVRQLLQEGQNDAALATVLVARQSHPA
                     DMNLVLIEGITLIRLQRYSDAAILLVDLARNNPRNAEINATLGAAMMGAGFYEEARES
                     LLAAIRLDKHLPECLYNLAQLYALVDPKDLKQARKYYKQALALGVMPDAQLEAILQ"
     gene            27544..28251
                     /locus_tag="PKI64_02030"
     CDS             27544..28251
                     /locus_tag="PKI64_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99818.1"
                     /translation="MKLRLAKWLVGVLLLPLAVALTMTFGRVVFILAQSPARLPMLPA
                     AACVAGIAIWAIIWLFLPPLTRTYVLGHELTHALWTILFGGRASRLRVGENGGSVQVS
                     KTNVWVTLAPYFFPLYTFMVALLWLLAVWLFPAVQPYAPIFLFWIGLTWSFHITFTLR
                     FLGFSQPDVREHGRLFSYALIYVINLATIGAALVAASSWTYRGAAADVLDNLVWLGQA
                     ARTFYGWAITRLPDFAA"
     gene            28346..29221
                     /gene="rfbA"
                     /locus_tag="PKI64_02035"
     CDS             28346..29221
                     /gene="rfbA"
                     /locus_tag="PKI64_02035"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOD99819.1"
                     /translation="MKGIVLAGGSGTRLYPITRVVSKQLLPVHDKPMVYYPLSVLMLT
                     GIRDILIISTPEDLPRFRALLGDGSALGLRLSYAEQPKPEGLAQAFLIGREFIAGEPV
                     ALILGDNIFYGMGLSGILHRAGALQSGGLIFGYLVNDPERYGVVEFDAAGKVLGIEEK
                     PAKPKSHYAVPGLYFYDGTVCDMAAALKPSPRGELEITDLNRKYLEKGQLRVELLGRG
                     FAWLDTGTHESLLQASNFVQTIEERQGLKIACLEEIAFRAGWISREQLRAQGEVMAKN
                     SYGRYLLELADGRHG"
     gene            29232..29801
                     /gene="rfbC"
                     /locus_tag="PKI64_02040"
     CDS             29232..29801
                     /gene="rfbC"
                     /locus_tag="PKI64_02040"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOD99820.1"
                     /translation="MQCTPTALPGVLIFTPQLHGDARGFFLQTYHAREYAAAGLARTF
                     VQDNLSRSCRDTVRGLHYQLRHPQAKLVSVLSGAVLDVAVDIRRGSPTFGRHVTVELS
                     AENRRQLFIPEGFAHGFRVLTDTADFYYKCTDFYTPGDEYGIFWNDPALGIPWGTIAA
                     PVVSAKDAALPHLRDLPTAHLPAFPADAS"
     gene            complement(29891..31978)
                     /locus_tag="PKI64_02045"
     CDS             complement(29891..31978)
                     /locus_tag="PKI64_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD99821.1"
                     /translation="MRALKYGIGFVVLAVGLGANAEEKVVEMAPIVVTASRIPEPINE
                     TASSVEQITGEDLFERQDRTLPEALSIVPGVLVQKTSYGQGSPYIRGFTGFHTLLLVD
                     GIRLNSPVFRSGPNQYWNTVDSFSLSHLELLKGPGAVLYGSDAVGGTLQVFSTLPEFA
                     APGVKAWGGRLAGRAASAERSVIGRAETAYATEHYAAQVGVTAKSFGDLEGGRHVGRQ
                     KKTGYDELNFDAKLRLALPGDREMILAFFSVDQDDIWRTHRTPWGISWHGTTIGTEPI
                     HRFDQDHDLGYVRYIDREATPFYDTLELTFYGQRQEETKEVQKQSGTSEVDGFDVRTW
                     GVKADLLKDTSFGTWAYGAEYIRDKIDSFRRSYDEDGEFVSRGIQGPVADDSYYHMAA
                     IYLQDRLALTERLELTAGARATYAKADIGRYENFETTPHTPASFEDDWTDLSGHLRLA
                     CQVIDEHWLVYASLSQAYRAPGLYDLTSFNVNRSTDIAIPSPDLDPEKFLGGEIGSRV
                     ESRYVAWHIAYFYTDLRDQIISHHIGDNYFSRANSGEGYVHGVETELKLKYTDQWQSR
                     FGFTWMEGYTDYTSTSGEPVHEYIRTLPMSGFATLRWQEPSKRFWVEGVVRAVAKEDR
                     LTANDKRDTQRIPPGGTPGYAVADLRAGWKVNQRLSLVGGVDNVTDKDYRQHGSGSNE
                     AGRNFILSAEYQF"
     gene            32079..>32402
                     /locus_tag="PKI64_02050"
     CDS             32079..>32402
                     /locus_tag="PKI64_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99822.1"
                     /translation="MEKSLSRIGGIFHAMEKSTAKVPHRGNIFIPHQRDFPRCGKIHG
                     KSSTLWKNRRQKFHTVEKSAPRHPIFSTQWNNFESTFPHNGTTLGHFFHTLMRRHVKR
                     SVLTDT"
BASE COUNT         6296 a        10543 c         8798 g         6465 t
ORIGIN      
        1 cgacttgatc tgcccccata aatagcttcg ggtgtcccat tgttgaactc aggtctgcta
       61 atctggaaca atttatcggc ataatttgta caataggcaa gcttatgcca ataaaactac
      121 caaaaacttt ccgaaaccgc tgttttcctt tccgaaaaac ggttttctta atcaaatctt
      181 aacaatttaa cgccccccta taccggcccc gccgtggcca cttatagaaa accgcacgtg
      241 tgcctcgcac accgcttacg caatttgttt atagccgctt ggctgttccg tacccttgcc
      301 gacccgaatt gtaatcccca gcaagctgtc ctttgccccg ccccaacgaa tccggctacc
      361 atctgccatc cccattctgt ttctttgcag ccttggcggc ttggcgagaa ccgttcccca
      421 cctttcgctt gcgttctttt gcgtctctcg cgccgcttgc gttaacgccc ccggctgttt
      481 tcccctcccc aaccaagcac gaagcacaaa gaaccaagca ccactaccga taaacctccc
      541 gccgatgccc gaccttgacc acccacaccg tcttttcttc atcttggatc gaatacaaaa
      601 tccgatatcg cccttggcgg attcgatagt attcctggcc cgtaagcttt tggactccgc
      661 gcggtcgtgg ctcttccccc aacccccgta tggccttcac gattttttga acatccgcct
      721 tgggcagcgc caataagtcc tgcttcaaga ccgattgccg cagcaacact tcatattttg
      781 ccatgacgct tgaggtcctt gagggcgtct tcgaaggata tcagcggttc ccgcgcccgt
      841 tctttgaacg cggcgatgtc ttcggcatct tcagccaagc tgatggctac cgcctcgttg
      901 accaaagccg atatcgaata ctccgtctcg gcggatttga tccgcaacgc cttgtgcagg
      961 tgcgggtcaa ggtacacagt agctcttttc ggcggcaaag ttggcatctc atcctccatc
     1021 ttgggcgaca ctataacgtc ataacgtcat aacgtcaatc tcttcttcgt cctattcccg
     1081 ccgttttgtt ttccggcctg aattttccca atcccgccgc cggcacagcg gcggcccccc
     1141 ccctccgacc taacccatct actttccttt gtggtcttgg cgtcttggcg agaagctttc
     1201 cccgtctttc ggttgcgttc ttttgcgcct ctcgcgccgc ttgcgttgac gtctctggct
     1261 gttccacctt caccctgcca ccatctcccc tttgcgctct tggcgtcttg gcgagaacct
     1321 gtcactatct ttcgcctgcg ctcttttgcg tcgtttgcgc cgtttgcgtt aatgccccgg
     1381 ctgtttttcc ctctccaacc aagcacgaag cacaaagaac caagtaccgc gttgctacac
     1441 gttgatatgc gtgcgcaatt ttttcagcgc cacggcgcct agggccagac gatggccgct
     1501 ggactggaaa attagccggc tgacgccatc atcacccagc cgcaccatct tcacctcggt
     1561 cacattgacg atcgtggagc ggtccagcgc ccggaagagt ttgcgcggca gcatgaaccg
     1621 ccactcgcgc agcgggcggt tgtgcaggaa ttcgcggccg tcgcgcacca aaacgcgcgt
     1681 atagacccgc tcgcccacga tggcttgcaa ctcccgtacg cggatcatgt ggcgatcatc
     1741 ccccaccttg aagctctgca tgtcgtccgg atcaagcccg ccaccttgct cctgcgccgc
     1801 aaggcgctcc cgcagccggg tgagtgcccg cgccaagggt tcctcgctga caggcttgag
     1861 caggtaatcc accgcgccca cctcatagcc cttgatcgca tagtgcgggt gcgaggtggt
     1921 gatcacgacc agcgggcggc gttccgccag ttccagcgcc ttgaaaccct tttgccggcc
     1981 aaggtgaata tcgagaaaaa ccgcgtcggg ctgctcttcg tcgaggagtt ccaccgctgc
     2041 gtccaacgtg ggcgcctcgc ccacaatatc cacatcgccg tggtcctcca gcagcgttct
     2101 gagattcagc cggtcaattt ccgcatcttc gatgatgaga atacgcatag gcggtgcaca
     2161 ttatcagctc ggccccgccc attccacccg gaaatgtgcg ccgccagtgg gccggttttg
     2221 cgcctgcacc cacccgccca gcaaccgcac gttggtttgc accacatgga gccccagtcc
     2281 cgcgccgtgc tccaaatcgg ccacggctgg cgagcctgtg gtgaaggcct caaacgtgct
     2341 gcccacttcg gccggcaggc ccgcgccttc atccagcacg tcgcaggcga aaaggtccgg
     2401 tgtgtgggtg aactgcaccg tcaccgcgcg cccggccggg gtgaacttca ccgcgttatg
     2461 cacgaggttg atcacgattt gcgccagccg caccgggtcg ctctgccgcg tttttccggc
     2521 cagcgcgccg gactcccagc gcaactccac ctgatgcgcc tcggcaatcg gctgcaaaat
     2581 atcgcgcatt tgctgtgccc attcggcaag gttcaccgta cgccaatgca cccgcggctg
     2641 gccgccttcc gtggccgaaa tatccagtac gttggtcagc agcaggttca agtgccgccc
     2701 gccggaacgg atttgttcaa gcatccgcag aaaatcttcc ggcaggccgc gccgctcgcc
     2761 cagccgcacc atggcttgcg acaaccccac cagcgcgcta agcggcgtgc ggatttcgtg
     2821 gctcatgttg gccaggaaca gccccttggc atagttggct ttttccgcgg cctctttggc
     2881 aatcaagagt tcctcgtgtt tggctcgcag agcctcttca gcctgcttgc gttcggtgat
     2941 gtcgcgggtg acgccgtgga gttcgatgcg gtcggttttt ggattgcgcc agaatcgggc
     3001 aatgacttca gtgtagatga ctgagccatc tttgcatggt tgggtggctt cttcagtgaa
     3061 gaagccggag aattcccgtc cctgttcgcg catctcgcgg cagaccgcgt tgacggtatc
     3121 gcggaatctt gtgacctgct gggggatcac tgaatcctcg aagggggcgg ccatgatttc
     3181 ttcgggggtg tatccacggg gattgataac agcagggctg acgtaggtgt aacggaaggt
     3241 ttcggtgtcc aggatccaga cgacgtcttt catggtttcg gtgagaaaac gatatttctg
     3301 ctcgctttcg cgcaaggctt cttcggcccg cttgcggtcg ctgatgtcgc gtgtcacccc
     3361 gtggatttcc acccggccgt tgttcggatt ctgccagtag cgcacaatcg cctcggtgtg
     3421 cacaaaagtg ccatccttgc gcggctgcag cacttcctcc gtgacgaacg aactgaacgg
     3481 ctgcccgctg gaacgcagga tttggaccag ccgttgcgtt tcggcgcgga tatgtttggc
     3541 ttgctcgggc gtcatggccg catccagcgg ctcggccatt acttcatccg gcgtatagcc
     3601 acgcagtttt tcgacggatg ggctcacgta cagaaagcgc aaggtctccg catccaaaat
     3661 ccacaccaca tccttcatgt tttctgtcag caggcggaat ttctgctcgg cggccaaaag
     3721 agcgtcctgt gccaaacgct gcgcagtgat gtcgcggacc accaccagat attcagccgt
     3781 gcgccccaca tccttgggca acgccgcaat cgccaactca aaccagccca accccgtgga
     3841 caacggcagc gaatacacgc tgccgcggtg gctgccatgg gcgactgctt cggccaatgc
     3901 ttccacaatg acgtccgccg cctccggcgg caatgcttca cgcacatttt tgcccaaaaa
     3961 catggccggc gacgcataaa ggatattagg ggtggccgca tggaactcca agatgacgcc
     4021 cgcggcatcc acccggaaaa tcaagtcggg aatggtgctc aacatggctt ccagccgctg
     4081 ttggttttcc tgcagattca ggttggcctc ggccagctcg cgcatggccg cgctcacttc
     4141 cgcggtgcgc tctgccacct gctgttcgag ttcagcagtg tgttcccgca gcaatgcatc
     4201 ggcttgggct tgggccaatg ctcggttaac ctgctcttgc cgcaaccggt cggccatggc
     4261 cgcctgaaag aggaaaaatc ccaccaacaa cccgacagtc ggcaacatat tggttggcgc
     4321 caccgtaggc caccagtgcc aatcctggcc gaattccagc accaccagca cccagaatat
     4381 ccaccacgcc aaaacataaa aacgtgccgt acggttcccg cgcttccatg ccatcatccc
     4441 aatcgccagt gaccccgccc ctgccaccag cgacaccaca tccatgaccg cctgtcgcaa
     4501 cgggaatggc cccgccgcca gccaaacgac caacaccacc acccccgcca tcgtccaccg
     4561 caaaccgcgg tccacccgcg gggactcctt cgccagcgag aaaaaccacc gcagataggc
     4621 caacagcgcc aggatcgcca acccgttgaa caccaaatgt ccgttgtaca ttacgtagcg
     4681 ccatcccggc catccccagt gccgccaatg gcccgaggtg cccaccagca ccagccacac
     4741 catgaccgca ctggccgcat aggtcaaaaa gccccaacta cgcgtaaata ccgcctgcaa
     4801 aatccccagc gcgcccagca ccgcgaacgc cccaaggcaa aaatagacga acgcatcctc
     4861 catttccgcc cagcgcagat aggccgccgg tgaaaacacg ctgaaccgga catccatctg
     4921 attgtctgaa cgcagccgca cataaacttc cacggtttca gcaggcgcca gttccaccga
     4981 tatcactgga ttgtggcgat tcaccgttcc gggcccgcac cgcgcatagc ggatgccgtc
     5041 cgccacatgc gtcacatcgc cccccgcgcg catcacatac caatccacct cttccgcact
     5101 gggatctggc cgtcgcaagt accacgtggc ccgctccggc atctgcgtct gcaacacaaa
     5161 atgcacccac acggcatcct gggtgaaccg ctgcacagcc gcctgctccc ccagcggcac
     5221 aaatttactg gccttccacg ccgccacagc ctccgccagc gtggccgacc ccgcgccatc
     5281 ccgccacact gccgtctgtt gacgcagctt caacgatgtc gtctccggcg tcaacaccgc
     5341 cggttcctgg cccgtcacca ctcgcgccaa acccgcgatc agcaggacca acaccatttg
     5401 ccgccatttg ctcacgcgcg gtatcgtgcc cgaaaccttc cccccggcgc aagccccgcc
     5461 ttgcccgcaa accggtttca ttccttcggc gccgactccc gcaaggccgc gtgtgcagcg
     5521 cgcaatccga ccaagcggtt ccgcaacaat gtgtgctgtg ccgtgccgac agcaaattta
     5581 tttttcattt tttgagtcac gcgaatcatc gccataatca cgcgcatcat ctcttgccgc
     5641 tcgctattcg tgatttgccg tcccacgctc cccgctcctg tcatccattc ctgcgcgtac
     5701 tgcaaggcgt ttagctgttc ctgcacccgt ttttccgccc ccgatcccac ccgtaactta
     5761 cccaacacct tctctaactt ccggatcaat gactccactg gtgccagagg ggatggccct
     5821 tgctttatcg tatttttatt tttggtatta ctgcgcatcg cgccacgtat gggatacata
     5881 ccattttatt tcatggagga aaagtagctt cccatcacga caagatacag cgccaaccgt
     5941 aattcggtgg tcaaaaggga gggattggcc tatgttcacg ccttgccatg ttgccgattt
     6001 atgccattcg tgaggagctg accgctacgt tgaaacgttc gcggcggatc atcctgacgg
     6061 cccccacagg ctccggaaaa tccacccaag tgccgcaaat gctgttggat tcgggggcgc
     6121 tgggcaccgg caaggtcgtg gtgctccagc cgcgccgttt ggcggcacgc ctgctggcca
     6181 aacgcgtggc cgaagaacgc cgcgtgccgc tgggcgatga agtgggctat caggtgcggc
     6241 tggaatcggt gtgcggacca aaaacacgca ttcattttga aaccgaaggc attttgctgc
     6301 gccaaatgct ctcccagcct actctgccgg gggtggccgc catcttgttc gacgaattcc
     6361 acgagcgcca cgtctatggc gatgtcaccc tggcgcaagc gctgcgtttg cagcaaacca
     6421 cccgcccgga tttgctgctg atggtgatgt ccgccacgtt ggatgcgcgc ctgctgcaac
     6481 cttatctcga accctgcgcc cacttgcatt ctgacggtcg cacctttccg gtcacgattg
     6541 aacatttggc caagcccctg cacccggacc acgatccgcc atgggatgcc gcggccaccg
     6601 cctacgaacg tgccgcacgc gccggcgaag cccccggcga tacgctcatc ttcatgcccg
     6661 gggcctacga aatccagcgc accatcgaag ccctgcgcca tacgccggcc gccaagggcg
     6721 cgctgctgct gccgctgcat ggcgaattgc cgccgcatga gcaggatgcc gccgtggccc
     6781 gccacgattg caaaaaaatc gtcgtggcca ccaacgtggc ggaaacgtcg ctgaccattg
     6841 acggcatccg gctggtgata gattccggcc tggcccgtgt ggcgcgcttt gatccgtggc
     6901 gcggcatcaa tacgctgctg attgaacata tttcacgcgc ctcagccgac cagcgcgccg
     6961 gccgcgccgg gcgcaccgcg ccgggccgcg ccatccgcct ctggaccgaa caggagcacg
     7021 ccggccgcgc cgcccacgat gtgccggaaa tcaaacgcgt ggacccggcg gaaatcctgc
     7081 tgaccctcaa agcgctgggc gtggcggatg tggcggcctt cccatggctg gagccgcccg
     7141 agccgcgcgc gcaggaccgc gccctcacgc tgctgcacga tctcggcgca ttggatgcca
     7201 ccggcgcgcc gactgctgtg ggcctgcgaa tgctgaattt ccccgtgcac ccgcgctatg
     7261 cgcggatgct gctggcggcc gctgaacgcg gcgcggtgcg ccccgtggca ttgatggcgg
     7321 ccattgccca aagccgcccg ctgctggtgc gcaacgcggg ccggcaggcc gtcgagcagc
     7381 gccgcgatat tctcggcgag gccggcggct cggacctgtt tttgctgatg cgcgcgtgga
     7441 attatgcccg gcataatcaa ttcgatctgc agcgctgccg gaagctgggc atccacgcgg
     7501 ccgccgcacg gcaggtcggc cccgtctggg attatttttt gcgcatcgcg gcggcacaac
     7561 aactgccgtt ggaatccact ccacccgaag atgccgacgt ggccaagtgc atcctgctgg
     7621 gattctccga ccaagtggct cgacggctgg accggggcac gctgcgctgc gaactggtgc
     7681 ataaacgccg cggcgaattg gcccgcgaat ccatcgcaga cgaagcccca ctgctggtcg
     7741 ccaacgaaat caacgaaatc ggccgtagcg atggcgaaat cagtgtgcta ctcacccaag
     7801 ccaccgccat ccgggaggaa tggctgcggg aattgtatcc cgacgatttc agcgcccgca
     7861 tcacggtgca atgggatgcc gccgccaaac gcgtggtggc ccggcaggag gtggtgtttc
     7921 gagacttggc gttggattcc cgcccctccg agccgccgcc cgccgaagaa gccgcgcaac
     7981 ttctcgccaa ggccgtcctt tccggcgagt gcgccctcaa gcagtgggat cgcaccgtgg
     8041 accaatggat tgcccgcgtc aatctcctcg ccgcggtctg ccccgactgg cagattccgg
     8101 cctacgacga agcctcccgt gaactgctga tccagcaaat ctgccacggc gcggtttctc
     8161 acaaggaaat caaggaccgc cccgtcctgt ccgccgtcaa gtccctgctc tctcccgcgc
     8221 aaattgcact ggtggaaaaa cacatgcccg agcgcgtcac gctcgccaac ggccgcgccg
     8281 tcaaggttca gtataccgac accgcggagc cattcatctc cgcccgcatc caggaattgt
     8341 tcggagtgga gtcattgccc acgctcgcct ccggccgcat cccgctcgtg atccatatcc
     8401 tcgcccccag ccagcgcccg gtgcagatca ccaaggacct ccccggcttc tggcgcgacc
     8461 actacccccg catcaagcag gaactccgtc gcaaatatcc caaacacgaa tggcgctagc
     8521 cgaatcaggt tccagccacc ccatcagtcc gttttattaa tttataggnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt agttttacct ttactcaatg attgtccaac
     8701 ggaccgagcg gttcatgatt tcatcgagaa acttgaactg tcggtcgtat tcttcagccg
     8761 atacttgtcc acgctgatat ttccttcgaa gggtgatata tcgcttgaat gcatccactt
     8821 gctctcgtaa atctggactc attacttgct ccgtaataac acgattagtc aatataccgg
     8881 gatattgaat gatgtcatcc aatggtgcgg cttctcgatc atactcctct tgtgtaatgg
     8941 cccgcatttc catacgcccg cgaagaatta atttcttctg aaatgcattc acccgcgcct
     9001 gcaggtccgg atcctcggcc atatgcgacg ccacttcggg gggattatcg ttcgaatatg
     9061 ctatgggttc gtgaataatt tcatccagag atgctttttg ccgcagaaat tcttcttctg
     9121 tgatgtgtcc agccttaaat tccctgcgta aggttatccg ctgaagaagc gcttcgacct
     9181 gtgcactccg gtttgtatcg gcatcctgtt cttgagtgga aagattttcg cttgcctgtg
     9241 gctcctgatt aaaaaccggg tctgtagttg cgtgtatatc tgtcatttgt tgagcatacg
     9301 cagctacaaa tacaaggagc aagcatgccc caagatgtac gatttttttc ataaaacaga
     9361 tgtccatccc atatattgct caataatccc attctctcgc caagcaattt gccggtgatc
     9421 tggacaaact aaaacgttgt cacaactttc ggttgggtaa gaatctccat taaaggcgct
     9481 gattggcgtt cgtattcctc atgggatatt tcgccatttt ccatctgatt gattatatct
     9541 tccaatttgg ttcctcgctg attccagtgg ccagaatcag gaaaatagtt cagtttcagg
     9601 ggtatgacaa caccagaaaa attgtttagc gaactgttgg gattaggcga tgcatgggtg
     9661 gtcgagcggg cgacgttcga cgaggccaag aatgctttag aactgcatgt gaaggaaacg
     9721 ccggagctct gggttaacga gcgctctccg cacgacggga gtacgggcgt ggtttgccat
     9781 gatcatgtgc gggggctacg ctggcgccat ctaaacatct tcagcaagga ggccgagatc
     9841 gtctgcgacc ttccgcgggg ccggtgtccg cagtgcggca aagtctggcg cgtgaccccg
     9901 ccatgggaag gtcgagcgaa gcacctcaca aaggagttcg aagccttcgc gctgacctta
     9961 atgcgggaga tgccggtgaa gaaggcggct gaaatccttg gtgagcagga cacccggatg
    10021 tggcgagtag tccaggccta tgtcgaggag gcgcatgcgg ccatcgatat gtctgacgtg
    10081 acctgcgtgg gcgtcgatga gatgactcgg gcgaaaggcc atcgctatct aacggtcttc
    10141 gcggatctgg tgaagaaggc cgtgctgttt gccacgccgg gaaaggacag cgaaacgtgg
    10201 gcggagttcg tgcaggctct ggcgatacat aacggccacc cccacgccct gacgcatgtg
    10261 tccatggaca tgagcaaggc atatgccaag ggggttcgcg attactgccg gaacgcccag
    10321 atcatctacg acaagttcca tgtgatcgcc catgccaacc aggcggtcga cctggtgcgg
    10381 cgggctgaag cacaaagcgg ctcggccacg gcccgggcgg tactcgtcaa gagccgctgg
    10441 ctgtggctga agaatcccga gaacctcacc cgcaaacaac gggcacccct gcggcagatc
    10501 gaccagaaca tgctccagac aggccgggcc taccagatgc gtctggtgct gcaagaaatc
    10561 tatgcgcttg attcggcggc cgcagcggcg gaacgttttg gggagtggtg ccgctgggtg
    10621 cgccgggagg cccggaaggc aagtcgtagg ctgcttcacc acatggcccg tgtagccgac
    10681 ctgatcgagt cgcacatcgc gggtatccta gcgcattggg agcagggcat aaccaatgcg
    10741 ttcatggagg ggctcaacag cgtcttctcc gccgtccgcc gcaaggctcg tggctatcga
    10801 agcatccgaa acatggttgc catgctctac ttcgttgcag gaaaacttcc ctcacccgcc
    10861 atactcagcc actgaaaata gcgaggaacc tccaatttgt ttgccgcatc cagccgttgc
    10921 tggatttcat aatcttcctg tgaaagcggc ctctcaacca ccccggtctc cttctgccgc
    10981 cggtaggttt cccactcctc atccgtcaac acccaatcga ccgccagcgt ggctttgtcg
    11041 cgcgcgaacc ggcatgccag agccagcacg ttggtcgtgg aatatacgcc gtttgtccag
    11101 taatagacat ccagcctttt tccacgatag atcccataac ccggagccag cacagtccag
    11161 gtcactccag tccccggcat ccgcaacggc gccaccacct ccacttccac cggatgggca
    11221 aacatctgcg cctccgccgt gtcgtaggtc ttgacctcct gcgacgcgtc cacgctccga
    11281 accaccacct tggcgatcag gtcgtactcc tcccacgact ccgccggccg cgccaccggc
    11341 tccgcccggc tgctgtggag ccagaacagc cccaagatca gaatcagccg attaaggaag
    11401 ttcctgcagt ttgtccgatt gccaccggta gtattcacgg atgggggtca ttctttcgtt
    11461 atactcttcc atactgatct cgccgccgcg ccattgccgg atcaattctc gtgcattttc
    11521 taccatatcg cgggattcac tgcgcggtga atccacgctc gacaccccac gctctttttg
    11581 gtcgcgatac tccgcccatt gcgcctccga aagaacccat tccgagacca gccatgtccc
    11641 gtttctccgc tggtatgaac aagccacagc gggttgattc ccggccgaat attccccttt
    11701 ggaataaaaa tcgaattcaa gccgcctgtc tccatgaatg ctgtagaccg gcgccagcaa
    11761 ctggattctg cccggctcca tttccggcgg catccgcaag gcctccgtca cctccacctc
    11821 cattgggtaa ccatacgatt ttccgcgggg tgaatcaaag taaagcacct cttgattggt
    11881 atgaactgtc aagatggtta ccttggcaat taaatcgtat ttcgtccaca gatcagccgg
    11941 cgcagcaacc atctcgccat aaccggataa tgctcctgtc ataatcgaca gtacaataat
    12001 gttgatgatt ttcatagttg catcctaatt acaattaatc ttgcctgaat acacttcatt
    12061 acatgcatca aatttgaagg ttgcggcccc atcaaattcc ggacagtgtg ggttatcttc
    12121 aaatttgatt tttgctgctt catactcggt taaaaactca tcaagtatta tttgcgcgta
    12181 atcacaagag tccgcaaggc tcaaatctgg tattttggca ttccattcgg ctacgaaacg
    12241 ttccagcagc gccagatgaa gctgctcgtg atctatggtt tctgtgattc tggtctgtgt
    12301 gcgcgccctt gaactgccta catccagatc acaattgttt gcggttacaa ccatattggt
    12361 cacaaccagt atccctacgt caattaggta gaaataaccc tctattctgc acagagagca
    12421 gtcgctccct tcacacacgg gtgccggtgt taggcaattc ttaattcggt actggatttt
    12481 tccccatgct ttttgatcgc cgtgatatag tggccatgca accatctggg gtgttggatt
    12541 tttcttttct ggttctggcg gctggtattt ttcatacccc gcccccgtta tctggccgac
    12601 cacttccggt ccagcagtga tttccgcgca atcggtgtcg cttggagtgg ctttggccga
    12661 gaagaatacg tctatggaaa acggcccctt gggaacggtg tatttgaatg atgccgtctc
    12721 gccctggccg gaagccggct ccgtcgtcaa accggcggtc gtccaattcc attcgatcgt
    12781 ctgggtgatc gcctcctcct caggcggctc gccgccacag ccttcgatgc tcggctcata
    12841 gtatttccgc ccgtcctcgg ccgtgtagcc cgccgtaatg ctgtcggtca tctctccgcc
    12901 ctccaatacg caatagcagt tgctttgcac cgtgttgctc atgctgaacg tggggcattc
    12961 ccgcgtgaga atccactcga acttcggcac cggcttgggc gggctgtcgc tgtcgcattt
    13021 ccagcaatcg ctttcaccgg ggtcgtgggg gtcgctgcaa tccacatcgg gatcgcccca
    13081 ggcgtcggga aaggggcgat cgggatcgcc ttcgtaggta aaggtttcgc cctcggcttt
    13141 caacgccccg gtccacgcca acgctgcgac gagcgcaacc ggaatccatt ggctcagttt
    13201 gctcatgctg ccacctcttc agtatttcag ttcagacagg ggtttggctt gctcaaacag
    13261 gctgcggacg tcctgttccg ctgcgccggc cacggtggta tcctggatgc gaacgttggc
    13321 ttcgctgctg atggacagcc cggcggtggt caggttggcg ctgcccaacc acactgtttc
    13381 gccatccacc acgaccagtt tcttgtgcat gactttgtag gcctgcatga agcggacctg
    13441 gacattgccc gtcgccagcg tgctggcgaa attcagcacc gcacgcttga gaaacgcgct
    13501 ggtgcccggc ccgaattccg tgacgaaggc gacctcgatt ccctcctgcg acttttgccg
    13561 cagcacgtca tagaactccc ccaagggacc gtagagtcct tcggacatgt tgtaggtggc
    13621 gatcagtatg ctcttgcgcg ctttcttgac gccttcgagc agatcggcgc cgaacgcggt
    13681 ttgcggcagg aaggcggttt taaccgttga accggaattg gcatccgccg ggcgtcgagc
    13741 aggaggagtg ctcgctgatg cgcgccacag caattgcacc gcggatttgt cgatccagcg
    13801 gatttggccg tccggaaagc gccactggtg gcgcaagggc tggccaacca gccattgccc
    13861 ggccagaagc cggccatcgc gaagcaggat tcggtcgccg ctttcggtca tgaattcctc
    13921 cctcacgcgg gtccagctgg cggcggatgg cgccggaacg gcttcggctt caagcaaagc
    13981 ggtctgttcc aggcgctcgt gggacgggag ggattgcagg cggcgcagga gatctatttc
    14041 cgcctcccac tgctggcggg tacgcatgtc gtccaccatc cggcgaccga gcacgtagtc
    14101 cgcccagcgg ggatcgggct gcggcagatc gagcaggacg cttgcttcgt atacccgctt
    14161 gccggagagt gcgccttccg accagttgtg ggagccgatc agaacccgtc tcaggtcggc
    14221 cacgaccagc ttgtcgtgct gggcgccatt ggggatgctc aaccgcacgt cgaccccggt
    14281 ggcacgcagc cgttccgccc aggcggagtg catatctccc agccggtcct tgccgccggg
    14341 gggcgtatag agtccgaggt agacatagac cccgcgagca gcggcgcagc tcatttcccg
    14401 cacaagagcg gcttgcgctt ctcccgccgt ttccggtgcc gggaaggcat acatcagcat
    14461 gagcaggctc tggtcggcgg ttcggatcac gtccagaagg gcaggcagat attgctcgtc
    14521 gggcagatag cgagcttggg cctcgaattc aaaagcctcg aacggcggcc tcgccaaggc
    14581 ggtgaaattc gtgcggggac cggtcggcgg caaacggccg cagccttccg ccggcacctc
    14641 gccggcggcg gctacgggcg gatcgaaaga catgcaatgg accgccacct gacccagaaa
    14701 ggttcggtag tacgcggcca tgtcggaaga atggaagcaa aggatatctt cgatgttcat
    14761 tcccatggcc gctttggtcc aattggccga tccggtccag acctggcggt cgtccaccac
    14821 cagcgtttta agatgcaggc tgtggcggtc gcctccataa attccgactt cgcagcccgc
    14881 ctcggccaag tcatgcagga ttcccatgaa cccgccctcg cgcatctgga tgtcgaacaa
    14941 ggcccgcaca cgtacttgag gattttgtgc ctggtcggcg agtgcctgga ccagttccgg
    15001 ctggttcaac agatacatcc ccacatcgat gcggttggtg gcggatcgaa tggacgctgc
    15061 caaggcttcg ccaacacccg gttcaaaagt gaaccaagca tccatgttcc cttccccggt
    15121 gcgggacaag acatccatcc tcgatggccg gaacgtccgg gagcggggac ctgccaggcc
    15181 ccgatggttc ccccccggcg cggcaggtac cggccagcgc atgtttcggt accgttttgc
    15241 aagctgacgc ggatcttcgc cgccataagc cacgatcggc gtcgcctcgg caatcagccg
    15301 gttcatctgt tcccggtact tctgcgccag aggcgcggag cgcatggaca ccgtatcctc
    15361 aatgttggcc tcgaacgcct gccgcgtcca attggccgtg ccgctgatca cgagatcctc
    15421 gtccaccacc atcgccttca agtgcatttt cccggattga accgtgacca cataggtttc
    15481 aatcccggcc agagccatgc gttccagaat cggccgttgg gcgggcttgg ccatctcgtc
    15541 gtcagtgatg aatcgcaccg ttaccccggg gcgttgtgcc actaggcaga gtgcgttgat
    15601 aagatcatcg tgggtcagga acgccatggc cacgtccact tgccgccggg ctctttgcaa
    15661 ggcgcccaaa atcaacgtca gggcatcccc cttgggcgaa aaggcgattt gggtttcctg
    15721 atactcaatc ggttgcgcat gcaaagtgca tggcataagg aggaacagcc ctgcaatccc
    15781 caaggcccat gcccgttgct tgaatgacgc atacctgtga gaatcggctt gcttttcagc
    15841 cgccttgcgc tcggaagcta atggatatga tgtgcagcgt gcagtgaaca gctcgcggct
    15901 tggcgagagt cataactctt tgcatttgat atccccgagt tctcgcattg caaaaatact
    15961 tatacgagac agcacgaggg gcggtcaact atcaagtctt ggtttccgtt tggcactttt
    16021 ccgtattttt ccgctttgtt tttcagctgg catgctgacg tctatcggtg cacccgctgg
    16081 gccaaatcgg ccaccccatt ttgcccgccg tcaagtccct gctctccccc ttgaatcgct
    16141 agatgccgca gacgcggtcc aaatggcggg tcaaaaccga ttgggcctgc gcccagccta
    16201 gtttcagttt gccttggtcg gtcaacaact cggccaactc acggatggcc tgttcgtgct
    16261 ccgcgcccgt gagcgtccgc aggcgttgac tcagctcctg acgccggcgg tccagatggc
    16321 gttgccaaat gcgtacaatc aaatcttgcg cggctttttc cacaccaaag tcggcatcgc
    16381 ccccgcgaat tttagccggc gcgttgacca cctgcgcggc gagaacctgc gcggcctcgc
    16441 cggcatcggc cactgccgcc atgaggtcgg gctcttcttc ggccaacgct tgaatgaccg
    16501 cccggcacgt gggatcggtc aacagcgggt aggggatcca ttggcgcacg aggccggcca
    16561 cttccggtga gccctgtgcg cccagcagca tggccaactc gacttcgtcc accggcgggg
    16621 cagcggtagg ggttgccgat gattccgcgg cggcggaagc cgggcggaac tttcgccgga
    16681 cggccgcgcg caagtcgcgg cgcaatgcgt catagccgat gcccagcccc gtggcggctt
    16741 cgcgcagcat ctgttcagct tgcaccgcgc tcggcgcacg ggtggccaaa tccaccgcgg
    16801 cacgcgtggc ccgcagcagg ccctccgggg tgcgcacgtc ttctttcgcg cgcagcacat
    16861 ccagcaaaaa gcccatggcc gtttgcgcct tgtccaatag cgcctgaaag gccgctgacc
    16921 cctgcccgcg gatcagcgaa tcggggtcct cgccagccgg cagcgccgcc agcgacaccg
    16981 ccaagccttc cacaatcagc agctcggcgg tgcgcagcgc ggccttttgg ccggcttcgt
    17041 cggcatccag tacaatcaag atgtgatccg catagcgctt gagcactgcg gcatgctcgc
    17101 tggtgaatgc cgtgccctgc gcggcgacgg ccgtcgtgaa cccgcccaga tgacagcgga
    17161 tgcaatcaat ttgcccttcc accagcagcg cctgccgcgc atccagaatg gcgcgtttgg
    17221 ctttgtccaa gccgaacaaa atgcggcttt tgcgaaagac cggtgtttcc ggcgtgttga
    17281 cgtatttgcc gacgtttttg tcgtcgcttt gcaatatacg cccgctgaag ccaacagcgc
    17341 ggcccagttc gtcgcggatg gtgaacatca cgcgcccgcg aaagcggtcg tagagatggc
    17401 cggactcatt gctcaccagc aggccggcgg cttccatggc ctcccgtttg atggcggcgc
    17461 gcggcccggc cttatccgcc agaaaaccat acccttccgg cgcccaaccg ataccccact
    17521 ctttccacac atccaggccc agctcgcgcc cgttcaggta atcgcgcgcg ggcgcggcat
    17581 cggcgctttg aatcaattct ttcaggtagc gtttggccgc ggcttcattg gcggcaaaca
    17641 actcgtcctt gggcgggcct tcccgggcgc caccgtgcgt ttcctccagt tgcagcggaa
    17701 tgccggcacg gctcgccaac atccgcaccg ccgtgggaaa gtccacgtgg tcatagagca
    17761 tcacaaattt gaaaacatcg ccgccagccc cgcagccgaa acagtggaac gcctgccgcg
    17821 ccggattgac gtggaacgac ggcgtttttt ccttgtggaa cgggcacagc gctttggccg
    17881 tgtggcctgt gcgcttgagc tgaatgtagg aacctattac gtcggcgatg tccagcgctt
    17941 ggcggatttg gtcaatcgta tccttggaga ccatggccgc catggccgat tattcctcca
    18001 ccggatacaa cggcgcgtac tcgtcgaggc cgaccacagg gtcgggctcc ggcagcggtg
    18061 gcaggatcgc gtgtggttct tccacgccgc cgggaatatc cgcctgaatc atccgtgcgc
    18121 gtgcggccac ctcgttatat ttttccacta ggaacggcag cgtggtgcgg ccggtcgcgg
    18181 agttatacat aacttcgcgc tgcacccatg ccgacggcac aaaacttgcc gcaagcaaaa
    18241 tcaccaaaaa aattacgccc tgtcgcaccg cacccaccag cagcccgccc agccgctcca
    18301 cccacccttt gaaggcaaag gtcatcagcg agcccagcgc aatccgcagc aggcgcattc
    18361 ccagcagcgt ggccagcgcc aacgccacca ccgccaccag ccgggtgatt tcctcattcc
    18421 acccccagcg cgcgcaagcc catgccgcca gcggctcata acccagccgc gtcaccagca
    18481 cggccaccac aatactgatc agcgtggcca gctcatgcga gagtccgcgg atcagcccca
    18541 tcaccgcgcc atagagcagc acggccacaa acaaccaatc cacccaattc catgttgccc
    18601 agtccatgct cgcctccttg cgtcagcttg ccaactccac gcccgcccgt ggcgccacat
    18661 caatctcgca gccgagcatc tgctcatcct cgaacaaaaa gcggccatga aacttgcggt
    18721 tggccagcac cagcggcatc cacacgcggt catcagccca catttggttg tagggcattt
    18781 cacgttcatc aatccacagc gggatggcct ccggagtttc ggtgggaatg ccgtcatagg
    18841 tggccgcagt gaacacgtgg caccgcaggg agtagccgtt ggtgaattca aagtccagca
    18901 cgccggcttc cgtcggctcg tagggcgtga ctttcagctc ctcctgcgct tcgcggatgg
    18961 cgccctgccg tggggtttcg cccgggtcca cgcgcccgcc cggcgcgttg atcaaccccg
    19021 cgcccatgcc ctgcaacttg cgaattagca gcaccattcc atccatgcgg atgaacatca
    19081 acgtcgccgt ttcccgcggg cgccactgcg cccaatcaat ctccgaaaat ttcacacgac
    19141 tccttccacc agcggccacg ttaccacacc cctgccccag cgggaaagcc gcatggtccg
    19201 cgcgctccgg cgcgttttac tacgcgcgtt gaatgaaccc gcccgtgcgg gtatccaccc
    19261 gcacgcgctc gccctgcgcc atatattccg gcacttgcaa ctccaggccg gtttccagct
    19321 tggccgtttt ggtgcgcgcg gtcacactgg cgccgcgcat cgcgggcgcg cactccacaa
    19381 tcgtcaactc cacggcatcg ggcaactgga tgcacagcac cttgccttcc tgagtgaggg
    19441 ccttgatgcc ctccatgccg tccaccagaa atggcaccgc gtcttcgatt tcgtcggcca
    19501 tcagctcgaa ctgcgtgtag tcatccacat ccatgaacgt gaagcggtcg ccctcgcgat
    19561 agctgaacga cacctgccgc gtgtcgaaat ccgtttcttt caccgggtcg tcgccgcgga
    19621 aggtctggtc cagcttggcc cgcgtacgca cgttgcggaa ccggatttta tacaaggtgg
    19681 ccgccccccg cgccgagggg gtttgcaccc gcagttgctc caccacgtgg gggtcgccat
    19741 tcagctccac cacgctgttt ttcgtcaaat cacacgcctt gatcatgcac ccgttctccc
    19801 ggcaaaaatt tcaaatttga ggctttttat cctttatcaa attagatttt tctgtaacag
    19861 atagtcaatg gcaagcttgc tttacgaagt atcatgcccc aaatgattgc agaacggagt
    19921 tggcggttcc ccaaccggat gcctgcgctg accaaggtgg tgcgggaggt ttccgcgtgg
    19981 atggatgagc tgccgttctc caatcgcgcc aagtattccg ccacgctgac cgtcgaggaa
    20041 atgggctcca acatcgtcaa gtatgcctat gacgacagcg acgaacacga aatccatttg
    20101 cacatggtgg ccaatcagga cgtcgttgaa atcacgtttg aagatgatgg ccatcccttc
    20161 gaccccactc aacagccgcc gcccgacatg gaaaaaattg tcgccgcgcc caaatccggc
    20221 ggcctcggca ttgagctggt ccggcgcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnng ccaccagcgt cacgcaagcc cgcgaagcct ttagcgccct cggccacctc
    20401 accctggttc ccgaccgcga aatcacccgc gcccatctcg tcgcggccga tgcgctcatc
    20461 gtccggtcca aaaccaccgt caacgccgaa ttgcttgccg acacccccgt cagctttgtc
    20521 gccaccgcca ccgccggcgc cgatcacttc gacatcccct ggctcaacga tgccgacatt
    20581 tgctggaccg cctccccagg ctgcaacgcc aacgccgtcg ccgaatatgt caccaccgtg
    20641 ttgacgctgc tggcgcgccg ccgcgccgca ctgctccccg gccgcaccct cggcatcatt
    20701 ggctgcggcc acgtcggcag cctcgttgct gaaaaagccg gcctgcttgg cttgcgcgta
    20761 ctgcgcaacg acccgccgtt ggccttgcaa accaacagcg ccgattacct cccgctggcc
    20821 gaagttctgc cccatgccga tatcgtcacc ctgcatgtcc cgcttgaaaa cggcggcccc
    20881 ttccccaccc gccgtcttgc tgattgccgc ttcttcgaga tactcaaacc cggcgcctgg
    20941 ttcatcaact ccgcccgcgg cgaagtcgtg gattccgacg ccctccaatg cgccctcgac
    21001 catcatctca tcgccgcctg cgcgctcgat gtctgggatg gtgaacccca tctggccgac
    21061 gccctcaaaa acgccgtgga tttcctcacc ccccacatcg ctggctatac cctcgagggc
    21121 ctgctcaacg gcaccctcca ctgctatcgc cacctttgcc agttcctcga agtggaaccc
    21181 acttggaccc ccgacccacg cctactccct cccgccccct tcatcgaaat tgatgccgct
    21241 caccgcgcgg atgacgacgt tctcgccgag ctactcgcca ccgcctgccc gattccttgc
    21301 gatacccgcg cttggcgcac cgacatggcc actcccgatc ccgccgaact tcgccggcgt
    21361 tttgaccgct tccgtaagaa ctacacttcg cgccgcgagt tttctgccat ccgcgtgcat
    21421 ctcgcccacg ccactcccgc tcttctcgaa accgtcgccg ccctcggctt ccaagtctgc
    21481 gcccactcgc catgaccgcc ccggtccagg ccctgaaact cgcgcttcaa ccgcccccgc
    21541 cctacaagac gaagccgtgg actgaaagcc ggcagaacca agcaggaaaa caggaaacac
    21601 aggaaaacag gaaccgaaaa cagccaacaa tccagatggt acggaagctg tctctccggc
    21661 tccaagctct aggtttcaaa attccattgt ctgctgtccg caggacgtgg atgcggagga
    21721 tttcttgcgg attttggcgg ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnccaagca ggaaaacagg aaacacagga aaacaggaac cggaagcagc caatagaccg
    21901 caggacatga ttcggttgtc cgctgtccgc aggacgtgga cgcggaggat ttcttgcgga
    21961 ttttggcggg gctgccgcgc aaaaataacc ggtagatttt ccgatgatgg cgcatggcgg
    22021 acgacccgag caggactcat gtccgcgagc ggtcgggcgc aatgcgccag cgcggaaaag
    22081 atgtcggttg gtttttgcgc cgccaaaata cacaagaaat cctcctacca actccctccc
    22141 cacccgctcc ctcctaccgc atgaccactg acgccacccc catcaccttc accgtcaccg
    22201 cccgcgctcc acgccttgac gtttggctcg ccgccgccca ccccgccatt acgcgctcgc
    22261 gctggcagca actaatcaaa gacggtcaca tcacgctcaa cggcacgccg ctgtgcaaac
    22321 ccaacacctc tctcgccccc ggcgacacac tcctcttcac cccccccgcc cccaccccct
    22381 ccccccttgc ccccgccgat atcccgctgg ctatccttta cgaagacgac gacctcctcg
    22441 tcatcaacaa acccgccggg ctcgtcgtcc acccagcccc cggccacccc ggcaacacct
    22501 tggtcaacgc cctcctgcac cactgcgccc acctgcaggg catcggcggc gagctgcgcc
    22561 ccggaatcgt ccaccgcctc gacaaagaca ccaccggcgt tttggtcgtg gcccagaacg
    22621 aagccgccca cgccgcgctc gtcgcgcaat tcgccggccg caccacccgc aaggaatatc
    22681 tcgcattggt gtggggcatc cctgccaagc ctcgcggcga aatcaaccaa cccatcggcc
    22741 gccatcccgt ccaccgcaaa aaaatggcgg tgaccgccaa aggtcgtgcc gccctcacgc
    22801 gttacgaagt catgggcacc ggcccacgcg ccgcgctgct gcggttggaa atcgaaaccg
    22861 gtcgtaccca tcaaatccgc gtgcatcttg cccatttggg ccatcccgta gtcggcgatg
    22921 ccacctatgg ccgcgcccgc tcgctccccc ccgacgcacc gcccattacg cgccagatgc
    22981 tccatgccca tcaactccac atcacccacc cccgcaccca cgcccccctc gtcttcaccg
    23041 ccccgccccc cgccgacttc ctcgcccttc agcaccacct cattcccccg ccactgtagc
    23101 cctccctccc gtaatcggcc agcttcccaa aatccaccaa aataacggtc tgatttcatt
    23161 ttttattata tgtgggaagg cctcatgata attatgtata tcccattgtc tatgttttaa
    23221 ttataaataa tatttttctt cataaatatc gtgtttttaa gtgccgattt cacttataat
    23281 tataagtgaa atcggcctca aattaatgcc ttggcccggc ggggaaaaag cccaaaacag
    23341 ggcggatggg gggcgggtct tgatttctga cgggaagacc ctagtattcc ggtttcgcgc
    23401 ttcgggcgcg gcaagaagga aaaagaccat gaaaattgta ttggcgtact cgggtggttt
    23461 ggatacgtcg gtgatcctgc gctggctgaa ggaaaattat caggcgcaga tcatcgcatt
    23521 cgcggcggat gtgggacagg aggaagagct gaagggcctg cgcaaaaagg cgctggcgac
    23581 gggggcgagc aaaatctaca ttgacgatct gcaagaagag ttcgcgcgcg atttcatttt
    23641 cccgatgatg cgcgccaacg cgatttatga ggggcagtac ttcctcggca cgtccatcgc
    23701 gcgaccgctg atcgcaaagc ggatggtgga aattgcgcgg caggagggcg ccacggccat
    23761 cgcccacggc gccaccggca agggcaatga ccaggtgcgt ttcgagttga cggcggcggc
    23821 gctcgcgccc gatttgcagg tgattgcgcc gtggcgcaac gcggcctatc gcgagcagtt
    23881 tccgggccgc agcgaaatga tcgcatattg cgcgaagcac ggcatcaatg tggaagcgtc
    23941 ggctaaaaag ccgtactcca tggaccgcaa tctgctgcat atttcctacg aggcaggcat
    24001 tctggaggat ccgtggttcg acgcattcgc gccgcaaaac cgcggcatgt tcaagttgac
    24061 cgtcgcgccg gaggatgcgc cggatcgcgc ggaaattgtg gagctggaat ttgtgcgtgg
    24121 gaattgcgtg gcggtgaacg gcaaaaagtt gtcgccgctg ggggtcatgc gcgcgctgaa
    24181 caagctggcg gggcggcacg gcgtgggccg cgtggacatg gtcgaaaacc gctacgtggg
    24241 catgaagagc cgcggcgtat atgaaacgcc cggcggcacc gtgctgcagt ttgcccaccg
    24301 gcaaatggaa tcgctgacca tggaccgcga aaccatgcac ctgcgtgacg ggctgatccc
    24361 ccgctatgcc gccatggtct acaacggctt ctggttcgcg ccggaacgcg tggcgctgca
    24421 agcgctggtg gaagaaaccc agcgcaacgt caccggcacg gtgcgcgtgc ggctctacaa
    24481 gggcaacctc tatgtggccg gccgcaagag cgccgtctcg ctgtataacc cgcaaatggc
    24541 gaccatggat gctgacccga ccaaggccta caaccaggat gatgccaccg gcttcatccg
    24601 gctgaacgca ttgcgcctga aggtggcggc caaggtgcag ggcaagcgta agaaatcgtt
    24661 ttaacccgcg cctgttttag gggttcaccg cgtggggcat cctcgggtag gatgccctac
    24721 cgcgataagt aataacaagt ataaggagcc aacatgaaaa tcctgattgc cgacaagatg
    24781 tccaagaacg cgattaccgc gctggaaaaa ctgggccaca ccgtgatctc caaccccgac
    24841 ctcaaggccg aggatctgcc gggcgcgatc ggcgatgccg aagtgctgat tgtgcgttcc
    24901 accaaggtca ttgccgccac catcgaggcc gggaaggagc tctcgctgat tatccgcgcc
    24961 ggcgccggcg tgaacacgat tgatctggcc aaggcgtcgg agtttggcat tcacgtgacc
    25021 aactgccccg gccggaatac cgaggccgtg gccgaattgg cgattggttt gctgattgcc
    25081 gccgaccgcc ggattgccga tgccaccgcc gacctgcgcg gcggcaagtg gcgcaaaaaa
    25141 gaatatggca aggcggcggg cctcaagggc cgcacgctgg gcatcctggg cgtgggctcc
    25201 atcggcaagg cggtggtgca gcgcgccaag gcgctcgaaa tgaatgtgat ggcgtggtcg
    25261 cgctcgctca cgccggaaaa ggccaaggaa ctgggcgtgg gcttctgcgc cacgccggcg
    25321 gatgtggcca aggtggcgga tgccatcagc ctgcacctcg cggccaagcc cgaaaccaag
    25381 aagatgatca acgccgagtt catcgcgttg ctcaagcccg gcgctatcat cgtaaatacc
    25441 gcgcgcggcg aaattgtgga ttggaccgcg ctgaaggatg cgctggcggc caagaaaatc
    25501 cgcgtggcca gcgatgtgtt tgatgccgag ccggccggtg gcgaggagcc cttcgccgat
    25561 acgtctctgg ccgcggccat ggcggcctgc accccgcaca tcggtgcctc caccgaccag
    25621 gcctccgagg ccatcgccga ggaagccgtg cgcatcgtca aggtattcca ggaaaccggc
    25681 aacccgccca acgtggtgaa tatccgcaag aagacctccg cggtgatcaa cctcgtggtg
    25741 cggcatttca acaaggtggg cgtgatggcc cacgtgctgg attgcctgcg tggggcgcaa
    25801 atcaacgtgg aagaaatgga aaacctcatc ttcgagggcg gcttggccgc gtgctgtacg
    25861 ctcaagctcg acaagaagcc cgaggccgcc ctcgtggaaa aaatcaacgc gggcgaacac
    25921 gtgatttctg ccgcgttgaa ataacccccg cccgaaagcg ccttgcccat gaaacgcatc
    25981 ctgctgctgc tggcgtgcgc cggctggctg atggccggcc cggtgcaagc cgaactgtcc
    26041 gaccccggtc aaattcaaac cgaggcctat atccagttgg ttcaggccga ccaaagtttg
    26101 gatgcgggcc gcttggacga ggcgctgcgc caatatcagg ccgcgcgcga ttactacgtg
    26161 cgcctggccc gggaattccc gggctgggag ccgcgcgtga tccaatatcg caagacctat
    26221 tgcgataatc agatcgccga tattcagcgc caacaggccg gcgggtcgcc gcgggaggag
    26281 tggccggaat tggaacccct ccccggcgag gcgccggtga cgacgccgcc cgcggcggcg
    26341 gttgtccccg aagagcccgc gcccgtggca gaccgctcgg tggaaatcaa ctacctcaag
    26401 agccgcattg ccagcctcga ggaagagctg cgcacgcgcg attcccagca agcagaatac
    26461 gacacgctcg ccgcccagta caagcagtgc cggcaggaat tggctaacgc caaccagcag
    26521 gtgcaggacg aaaaaaacgc cggccagtcc gcgctggccg cgctgcaagc cgaggccgac
    26581 aagaaggacg agcaactccg cgatctgcgc aaggagctca aatccaagaa acaattggat
    26641 caggccctga acgatctgga agccaaggtc aacgaactgc aagcgcagaa ccagcggctg
    26701 gatgatgaaa ttcaggcgct caatcgcgag ttggatgatg ccgaaatccg cgctgatcag
    26761 gccgagctga aattgcaaca agccgaagct tcgctcaagg cagccaacaa ggctttggaa
    26821 aaagcggaga aagccgccca ggctcgcgtg caaaaagatg agccggctcc cgccaaaact
    26881 caagaggccg ctgccgcaaa gccatcgcgg aagccggcgg tggcggatgt gcctatgccg
    26941 ccggtcgccc ctgaaaaagc gccgcccgca gaacagccgg cgccccccgc cttgccgccg
    27001 cccgtcaagg tgaccagcac cgccgcgccc cgcgaagtcc ccgcgggaat gaacgcggcc
    27061 gacttcgtgc gccagttgtt gcaggaaggc caaaacgacg ccgctctcgc caccgtgctg
    27121 gtagcgcggc aaagccaccc ggcagacatg aatctggtac taatcgaggg catcaccctg
    27181 atccgcctgc aacgctattc cgatgccgcc atcctgctcg tggatttggc gcggaacaat
    27241 ccgcgtaatg ccgaaatcaa cgccacgctg ggcgcggcca tgatgggcgc gggcttctat
    27301 gaggaagcgc gcgaaagcct gctggccgct atccggctgg ataaacacct gccggagtgc
    27361 ctctacaatc tggcgcaact gtacgccttg gtggacccca aggatctcaa gcaggcccgc
    27421 aagtattaca agcaggcgct ggcgcttggc gtcatgcccg atgcccaatt ggaagccatt
    27481 ctccaatagg cgcggcgacg ggttgcccgc ccaagatcag gccgcgccgg aagactcccg
    27541 ccgatgaaac tgcggttggc gaaatggctg gtgggcgtgc tgctgctgcc gctggccgtg
    27601 gcgttgacca tgacgttcgg ccgcgtggtg ttcatcctgg cgcaatcccc cgctcgcctg
    27661 ccgatgctgc ccgccgccgc ctgtgtggcg ggcatcgcca tctgggccat catctggctg
    27721 tttctgccgc cgttgacgcg cacctatgtt ctcggccatg agctgaccca cgcgctgtgg
    27781 accatcctct tcggcggccg ggcttcgcgc ttgcgcgtgg gcgaaaacgg cggcagcgtg
    27841 caggtcagca aaaccaacgt gtgggtgaca ctggcgccct atttttttcc gctttacacc
    27901 tttatggtag cgctactgtg gctgctggcg gtgtggctgt tccccgccgt gcaaccctat
    27961 gcccccattt ttttgttttg gatcggcctc acgtggtcgt tccacatcac cttcaccctg
    28021 cgctttttgg gtttcagcca gccggatgtc cgggagcacg gccggctgtt ttcctatgcg
    28081 ctgatctatg tcatcaacct ggctaccatc ggcgcagcgc tggtggcggc cagctcgtgg
    28141 acgtaccggg gcgcggcggc cgatgtgctc gataatctcg tgtggctggg ccaggctgca
    28201 cgcactttct acggctgggc catcacgcgt ctgccggact ttgcggcatg acgcgcacgc
    28261 cagcgccgcc cggcgcaaaa cggcttgaag cctgcaccgc gttgggccat gcttccgtcc
    28321 ggttgtcacg gaaaggattc aacacatgaa gggcatcgtt ttggctggtg ggtcgggcac
    28381 gcgcctctat cccattacgc gggtcgtgag caaacagctc ttgcccgtac acgataaacc
    28441 catggtgtat tatccgctct ccgtgctgat gttgaccggc atccgcgaca tcctgatcat
    28501 cagcactccc gaagatttgc cgcgtttccg cgcgctcttg ggcgacggct ccgcgctggg
    28561 cctccggctc tcctacgccg agcaacccaa gccggaaggc ctcgcgcagg catttttgat
    28621 cggccgtgaa ttcatcgccg gcgagcccgt ggcgctgatt cttggcgaca acatttttta
    28681 cggcatgggc ctgtcgggca ttttgcaccg cgccggcgcg ttgcaatccg gcggcctgat
    28741 tttcggctat ctcgtcaacg atcccgaacg ctacggcgtg gttgagtttg atgccgccgg
    28801 caaggtgctg ggcatcgagg aaaaacccgc caagcccaaa tcccactacg cggtgccggg
    28861 cctctatttt tatgacggca cggtgtgcga catggccgcc gcattgaaac cgtccccccg
    28921 cggcgagttg gaaatcaccg acctgaaccg gaagtatctc gaaaaaggcc aactgcgcgt
    28981 ggaattgctc ggccgcggtt ttgcctggct ggataccggc acgcatgagt cgttgctgca
    29041 agcctccaac tttgtgcaaa ccatcgagga gcggcagggc ttgaagattg cctgcttgga
    29101 agaaattgcc ttccgcgccg gctggatttc ccgggagcag ttgcgcgcgc agggcgaagt
    29161 catggccaag aattcgtatg gccgctacct gctcgaattg gccgatggcc gccacgggtg
    29221 aagccatggc cttgcaatgc acccccaccg ccctgccggg cgtcctgatt tttactccgc
    29281 agttgcacgg tgacgcgcgc ggattctttc tgcaaacata ccacgcccgc gagtatgccg
    29341 ccgccggcct cgcccgcact ttcgtgcagg acaacctgtc gcgttcctgc cgcgataccg
    29401 tccgcgggct gcactaccaa ctccggcatc cgcaggccaa actcgtcagc gtcctgagcg
    29461 gtgcggttct ggatgttgct gtggatatcc gccgcggctc gcccaccttc ggccggcacg
    29521 tcacggtgga actctccgcg gagaaccgcc gacaactgtt catccccgaa ggcttcgccc
    29581 acggcttccg cgtgctgacc gataccgctg acttctatta caagtgcacg gatttttata
    29641 cccccggcga cgaatacggc attttctgga acgaccccgc cctcggcatt ccatggggca
    29701 caatcgccgc gcccgtggtc tccgccaaag atgccgcctt gccccacctt cgcgacctcc
    29761 ccaccgccca cctgcccgcg ttcccggccg acgcttcata acgccaccct ccctccggca
    29821 agttgtcccg ccaaaaaacg ggggcggtgt gcatccctgc gccaccgccc gccgtgctgt
    29881 cgccgttgcg ttagaactgg tattcggcag aaagaatgaa gttccggccg gcttcgttgc
    29941 tgcctgagcc gtgctggcgg taatccttat cggtcacgtt gtccactccg cccaccagcg
    30001 agagccgctg gttgaccttc cagcccgcgc gcagatcagc cacggcatag cccggcgtcc
    30061 cacccggcgg aatgcgctgc gtatcccgct tgtcgttggc cgtcagccgg tcttctttgg
    30121 caaccgcgcg gaccacgccc tccacccaaa accgcttgga tggctcctgc cagcgcagcg
    30181 tggcaaaacc gctcatcggc agcgtgcgga tatattcgtg gacgggctcg ccggaggtgg
    30241 atgtgtagtc ggtgtatcct tccatccacg tgaacccgaa gcgggattgc cattggtcgg
    30301 tatatttcag cttgagctcc gtttccacgc cgtggacgta gccttcgccg ctgttggcgc
    30361 ggctgaaata gttgtcgcca atgtggtgcg aaatgatttg atcgcgcaaa tcggtataga
    30421 aatacgcgat atgccacgcc acataccggc tctccacccg cgaaccaatt tcgccaccga
    30481 ggaacttctc ggggtccaga tccggcgaag gaatggcgat gtccgtgctg cgattgacgt
    30541 tgaagctggt cagatcgtag agcccgggcg cgcggtaggc ctgcgacaac gaagcataca
    30601 ccagccagtg ttcatcaatc acttggcacg ccagccgcag atgcccggaa aggtcggtcc
    30661 agtcgtcttc aaacgatgct ggcgtgtgcg gcgtggtttc aaagttttcg taacgcccga
    30721 tgtcggcctt ggcgtatgtt gcccgcgcgc cggcggtcaa ttccaaccgc tccgtcaggg
    30781 ccaggcggtc ttgcaaataa atggccgcca tgtggtaata cgagtcgtca gccaccggcc
    30841 cctgaatgcc gcgcgaaaca aattcaccat cctcatcgta tgagcggcgg aagctgtcaa
    30901 ttttgtcgcg aatgtattcc gcgccgtagg cccaagtgcc gaagctggtg tccttgagca
    30961 agtccgcttt gacgccccac gtacgcacat caaagccgtc cacttcgctc gtgccgctct
    31021 gcttttggac ttccttggtt tcctcttgcc gctggccgta gaacgtcagc tccaaggtgt
    31081 cataaaacgg cgtggcctcc cggtcaatgt aacggacata ccccagatca tgatcctgat
    31141 caaagcggtg aatcggctcg gtgccgatgg tggtgccatg ccacgaaatc ccccacggcg
    31201 tgcggtgcgt gcgccaaata tcgtcttgat ccaccgagaa gaatgccaaa atcatttccc
    31261 ggtcgccggg caacgccaag cgcaacttgg cgtcaaaatt aagctcgtca tagcccgttt
    31321 tcttttgccg gcccacatgc cgcccgcctt ccagatcacc aaacgatttc gcggtcacgc
    31381 cgacttgcgc ggcgtagtgt tccgtggcat acgccgtttc cgcccggcca attactgaac
    31441 gctcggcgga tgccgcccgc ccagcaaggc ggccacccca tgcctttacg ccgggcgccg
    31501 caaactccgg cagcgtggaa aatacctgca acgtgccgcc gaccgcatcc gagccataca
    31561 acaccgcgcc gggacccttg agcagttcca aatggctcag cgaaaagctg tccaccgtgt
    31621 tccaatactg gttcgggccg ctgcggaaaa ctggcgagtt caggcgaatc ccgtccacca
    31681 gcaaaagggt atggaacccc gtgaaaccgc gaatgtaggg cgagccctgc ccgtaggatg
    31741 ttttttgcac cagcacgccg ggcacaatcg aaagcgcttc cggcagtgtg cggtcttggc
    31801 gctcgaaaag atcctcgccg gtgatttgct ccaccgaact cgcggtctca ttgatcggtt
    31861 caggaatccg cgacgccgtc accacgatcg gcgccatctc cacaactttt tcttccgcat
    31921 tcgcgcccag cccaaccgcc agaacgacaa acccaatgcc atacttcagc gctctcatca
    31981 gacccaacct ccttggattt atgattttag agatataatc gccgtccccg cgcaaaaagt
    32041 tcaccacccc ccataatttt tttcaagttt tccacaccat ggaaaaaagt ttatcccgca
    32101 tcggcgggat tttccacgct atggaaaaat ccacagcaaa agttccacac cgtggaaata
    32161 tatttatccc gcatcagcgg gattttccac gctgtggaaa aatccacggc aaaagttcca
    32221 cactgtggaa aaatcggcga caaaagttcc acactgtgga aaaatccgcg ccccggcacc
    32281 cgattttttc cacacaatgg aacaactttg agtccacttt tccacacaat ggaacaactt
    32341 tgggccattt tttccacact cttatgagaa ggcatgtcaa gaggtccgtt ttgacggaca
    32401 cc
//
LOCUS       DAOPNP010000011        32201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1818_length_32201_cov_8.627947, whole genome shotgun sequence.
ACCESSION   DAOPNP010000011 DAOPNP010000000
VERSION     DAOPNP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 32201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32201
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_32201_cov_8.627947"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..3853
                     /gene="smc"
                     /locus_tag="PKI64_02055"
     CDS             272..3853
                     /gene="smc"
                     /locus_tag="PKI64_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOD99823.1"
                     /translation="MYLKAVEMIGFKSFAERTKMMFEPGMMAIVGPNGCGKSNVSDAI
                     RWVLGEQSPKALRGGTMTDVIFNGTDQAKPLAMAEVSLTLTDCEAVLGVEYNEVTVTR
                     RVFRSGEGQYFINKTPCRLKDIQRLFMDTGLGTDSYSVMEQGRIDQILSSRPDDRREV
                     FEEASGISKYKADKREALRKLEHTEANLLRLSDIIKEVKRQIISLQRQVGKARRYKNL
                     QEQLRASDVWLAKGRLSELDLALATLEKELGQHQATEDGLRQKVEGAEAAAGALRANM
                     AAVEGRIAAALDASSEASGALARTQELIRVNGERAQELETLSQRDSRESASASASLAS
                     HQAELDVLKKDLIAATDAHETARLELTKRQAALTAHDQQTDAARKRLQDLRTEALELE
                     SRLARLQNEWNEIEARERTDLIRRERCAAEKAEAERAVESYAERLALMAAQRAQKQTA
                     AEQAQQQVAALRASQADRATQLAQVQQELSRLASASAAREAQVALLTRSREEARGFPA
                     GAKKLLEGATVPGVNPKDILGALAQKVRAPKEDQLALEAVLRSWLDAVLVRDAAAAVA
                     IARQVQAAAGGAVRLLALEAGEVPDTPPGAGEPLLPRVKAAADLAGLLRRLLGAVRVV
                     PTAADMPAAIPAGLTYVTRDGLVARGDGSVEFWAPGENESNPLAREHQLAEWQEDLAR
                     LRQEVENHNAQAAALQQDEQAARESMAAAQTAWEAQQREMALAEGEIRMVEREAKQAG
                     ERAETIGWELAALLKKTDAGTQRRSEMAGEMEDIRQRQAAVRTQTTADSETLRKLEDE
                     RNARLEEATECRVALAGYKQQVDGLRARGEPLEARLRELTALISERAAGIDSYRERIA
                     ALAAATANAQQQLAPLNDEVARCQAELQEARALRDRQNAELTQSDAALHSLRAELDTI
                     REQRNTMEVQLARHRLHRQNLVERMSAEYSLQPAEILKAKEPEWEEDAKPADREALET
                     MVAEIRTKLQSMGPVNLVAIEEHQELEERYAFLNQQNDDLLSAKQQLLDLIRKINETT
                     TVLFTDTFNKVNDNFQELFKQLFGGGNAKLVLVDEENVLESGIEIIARPPGKKLQTIS
                     LLSGGERTLTAVALLFALFKVKPSAFCLTDELDAALDDSNISRYLEMLKGFLKDTQFI
                     VITHNRQTIGSADALYGVTMEKQGISKVVSVKFRGKEKPPAADDTPA"
     gene            complement(3871..3947)
                     /locus_tag="PKI64_02060"
     tRNA            complement(3871..3947)
                     /locus_tag="PKI64_02060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3911..3913),aa:Val,seq:tac)
     gene            4233..5504
                     /locus_tag="PKI64_02065"
     CDS             4233..5504
                     /locus_tag="PKI64_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HOD99824.1"
                     /translation="MKRSLYPYSGRQTILGIQMLFVAFGALVLVPLLTGLDPSIALFT
                     AGVGTLLFHAITGGSVPIFLASSFAFIAPIQFAMKEFGTGATLGAIFFAGLVYLIFSL
                     MARIGGREMLNRYLPPIVTGPVIMVIGLKLAPVAVGMTRNFDGSAALDGRALFLATCS
                     LLTAVAAVMFGKKLLSLIPILCGIVVGYLVAIAMGVVDFSIIREASWFQLPWTLAREN
                     GSFAMPRFDPAAILYMLPVALAPAIEHIGDILAISSVTGRNYLTNPGLHRTLLGDGLA
                     TSLAGLLGGPPNTTYSEVTGAVALTKAYDPVLMRIAAVVAMILAFAGKLGAILRTIPG
                     PVMGGIMVLLFGMIAAIGINSLVKARVDMSKSRNLVIASVILTSGIGDMTFTCGRFCL
                     SGIGLAGLVGVLLNLLLPGPREAHEEQPENI"
     gene            5627..6544
                     /locus_tag="PKI64_02070"
     CDS             5627..6544
                     /locus_tag="PKI64_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOD99825.1"
                     /translation="MKRVSVATGLDYRQRVCRAMDYIHAHLNRELSLAEIAQAASFSA
                     FHFHRIFSAVTGETVFGFMRRLRLERAANQLLGHPQSDITSIALANGFSSSQNFAKAF
                     RAHFGCAPSAFRRRKDGHTKRKEREVFSLRLAQDARQVKRTSPQPERKWTMQKVEVKT
                     MPAWHVAYVRRLGPYGKATCEAAFQELMRWAGPRQLLGKGPMLSIYWDNPEVTAPANC
                     RVDACLVVPAGTPVEPPVALQDVLGGPYLVCAFNLPPTDFTAAWTEAFQYLVQKGYEC
                     ADRPCYELYYDDCTGATCRFDICIPLLQK"
     gene            complement(6666..7070)
                     /locus_tag="PKI64_02075"
     CDS             complement(6666..7070)
                     /locus_tag="PKI64_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99826.1"
                     /translation="MSAGKTRLERWEARLKKVFDEIDTELELRYGHRARRHPNRPPHG
                     ATANREHDGLFDLGAAFTVGIGSQYGPGYVVQARIATLTAIPPAIQQELEDMVARRLR
                     EKLPAAFPGHTLHVDRDGPIYKIHGDLSLGTL"
     gene            complement(7067..8536)
                     /locus_tag="PKI64_02080"
     CDS             complement(7067..8536)
                     /locus_tag="PKI64_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOD99827.1"
                     /translation="MSEPLQPSHGDYLRDESRRTGHAEQIVFPRSEEDVLAALAEARA
                     NRWPVTVQGARTGVAGGAVPEGGLILNLSRLTAIEAAGDTLRVEPGATLNAIRAATPP
                     GYFFAPDPTETTASIGGMIACNSSGALSFLHGPTRNHVLGLRVALASGEVLDLRRGRD
                     QARGRAATLGAWQGELPALPQPAVKNAAGYFTAPDMDLCDLFIGAEGTLGIVTAATLR
                     LLPAPAAIVGLMMFLPSTAAVVDLVNALRAGRTASPAPLAALEYFDSRCLRFLQDRAA
                     ILASKGIAVPALPPGAACVYAEWHAPGAAAAEAAMLAAAEQLPDLGANPDACILADNP
                     HELEKLKLFRHAVPELVNAEIDERRRQHPGIIKLGTDMSVPDDHLADVCALYERDLAA
                     TGLDHLIFGHIGANHLHVNILPRNADDYAAGRELYARWAAQIIAWGGSISAEHGIGKL
                     KRDLFRQMAGEKAIADMRKLKQHLDPTGLLNPGTLLPAP"
     gene            8608..10287
                     /locus_tag="PKI64_02085"
     CDS             8608..10287
                     /locus_tag="PKI64_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOD99828.1"
                     /translation="MTKRMVKVKFEHGPEQDVEAGIALSQILAQFPAVKEGLEFIPLG
                     ALVNNEFVSVEYRIETDSTVRLLGYRDSYGQRIYRYTIAFLLAKVAHELFPGCDFAVE
                     HSLSKGLYCSFRLGDVPGISAEQLAKLDAALRKLLDDKAPIDRIKITYDEAIAHFEAA
                     GAKDKLNLLRFKNSSKVTVYQCGRFMDLANQPLANNAAALVNYQLIPYQQGFVMMGPD
                     RMDPTTFPPFEPAHYIYDVFKGHKDWGRIVRVRTVGDLNERIARKKIDDFIDVNEAYQ
                     EKRIALLAEQIVQRRGQVKWILIAGPSSSGKTTFSKRLSLQLKASGMNAKPISLDNYF
                     VGRDKTPVDENGEVDFEHIEALDLDLVHEQLAKLDSGERLELPYFNFHTGQREWKGEM
                     MHIGPDDVVVLEGIHGLNPRLTSSIPVEHKFRIYISALTQLNIDSNNRISTTDNRLIR
                     RMVRDNNFRSHNALRTLQMWPKVRRGEKTWIFPFQKEADVAFNSALDYELAVLRPFAE
                     PLLAEIKPSMPEYAEAVRIQQFLSNFLVISPDRVPPTSVIREFIGKSFFQY"
     gene            10387..11763
                     /gene="der"
                     /locus_tag="PKI64_02090"
     CDS             10387..11763
                     /gene="der"
                     /locus_tag="PKI64_02090"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOD99829.1"
                     /translation="MTTPTPTNSPARMVAIVGRPNVGKSALFNRLVRRRLAIVHSEEG
                     VTRDRLLARATWLGQTFQVVDTGGLALMDRAKTGDDIQRATAEQVMAALADSAAAILV
                     VDITAGIQPLDVEVARLLRQAGRPVVLAANKADLPEWDDHTDDFARLGFPVVAISAAH
                     NRGIEALLEEVLPHLPPVGGEAEPPRLKVAVVGRPNVGKSSYINKLTGQQRVIVSDIP
                     GTTRDTIDVPFTLRTATGEQHYTLVDTAGMRRRGRVHQSVEKFSLFRTEQCIAEADIV
                     VLMLDAAEGPGAQEKKIAAMIREHHRACVVLVNKWDLATGSGVKAEDYAARMRADLFF
                     LDDIPILFVSAASGYHIRRSFEVIATVAARLSTKMPTGVLNRVLRDAFMRSQPPFTRG
                     RQLKFYYATQTGLNPPRVTLFVNDPTLNTPTHQAYLTARLRAAFDLAGVPLVLRYRSS
                     HGAPEEPT"
     gene            11854..13212
                     /locus_tag="PKI64_02095"
     CDS             11854..13212
                     /locus_tag="PKI64_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD99830.1"
                     /translation="MWRVAAPIIISMASFTLMQFCDRIFLARYSSVTLRAALPGGILA
                     FTLTAFFQALAGYAGTFVAQYHGAGRREECGRATAQGIWLGLLTWPLCIALIPFGDWL
                     LSVAGHPADVLAAEQTYLGILMLGGGIHAITNALAGFFSGRGDTRTPMLASLLANLAN
                     IGLDYAMIFGHWGFPEWGIAGGAIATVIASALGMAMLLVLYYQERFRREYGTWRGRRL
                     DWQRMGPLLRFGVPAAINTVQDVGAFAFFVVLLGRLPPVEMAASNIAFSINNVAFMPL
                     LGMGMAATIVVGQHQGARNPATAARAGWTALKLSWFYMGAIALTFLLLPRMYFALFTG
                     EGPDVLHMDEVIGLGRWLLVLMALWGMLDAINLVVAGSLKGAGDTRFVLLYSAVVTWG
                     VWIPGELVLVLWCHAGLLATWLWMSVYVFLLAGGLWLRFLRGKWKHIDMIRQPHPELP
                     AE"
     gene            13261..14556
                     /locus_tag="PKI64_02100"
     CDS             13261..14556
                     /locus_tag="PKI64_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOD99831.1"
                     /translation="MEKETGPNVWDDAIAQDVNAGIRAWRARVLQVLLIFSSVLLFVP
                     LVAVWAGWGMELTRPLRVLFALVYLLLVVTALWRRGPFAWRLGVLLGLLALVGFLRLL
                     IGRLDGSGRITLLLLPLLALVLAGPRVGWAMVGVAVAEYVAVPLLLQSGWLAGRGLAW
                     TATDRSPAFWGLQGIFGLVVLLTLMFLFTRFHARLFGAMRAEYQARRQLEVETADRQR
                     LEAAIVRVDEEERRRLGSDLHDGLCQHLTATLLNCSLLEKRWAEAGQPDAVALGQVRQ
                     SIADSIGMAHDVAQGLCPMDLAPDALLPALEHLCRETREHHGIQCRLQAPTAVAIRRP
                     EDAMHWYRIAREAVANAIKHARCTRLDLTLEHDGDDLVLRICDDGPHTPPDTVPVAGL
                     GLNILKYRAQLLGGVLTVAGGPAGGMCVTCRAPQGGKSA"
     gene            14553..15191
                     /locus_tag="PKI64_02105"
     CDS             14553..15191
                     /locus_tag="PKI64_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD99832.1"
                     /translation="MTKAEPIRIFLVDDHPLVRAALEQLLAGHGHAVSGQAGSPAEVL
                     AHPALTRSHLVVVDLALDKENGMSLIPALRARRTPVLVYSMHESAHIIRQALAAGALG
                     YVTKREAADSLLAAIHAVRQGETYLSPRAAAALRTESPLAGLSGQQLQIYRRLGQGLA
                     NEEIARQLGISVRTLESYCVRIMDKLGVQGAKELRQHAIRAAIATGPDQPEP"
     gene            15587..16564
                     /locus_tag="PKI64_02110"
     CDS             15587..16564
                     /locus_tag="PKI64_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99833.1"
                     /translation="MRYKDKTMWDTIKLTRIQSLPGKKFWKSLSPTFLHFGEQEELCA
                     AHEAVLKSEFQHSWQQATESIICGLAGRDQCKKYTRSRLKKLGRRRKDKAQSWEYILP
                     QSVLLHAWLAFRMAEAAAKQTYTSNPIQIDGGDNHQMLNLVSQRYTPENRSNVDACLD
                     NDSKAIGLWMVDYVRTVLIPRINELARRYFGKAHADIPEYFPLKYKIYYTEPSNYWHN
                     PDKKGCIRLLESWLSKTIRGPSSDLECVSAIDVFLNYVSSVNHVYSHFTFVKELYAFF
                     NEDSEIYRTLENGVCPGAFHAVRQRILNISIGDISKPLFLRQKLSPPRK"
     gene            16675..17346
                     /locus_tag="PKI64_02115"
     CDS             16675..17346
                     /locus_tag="PKI64_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99834.1"
                     /translation="MTESEAKFEAYCSVNAIPYTRIAVGEDKTPDYEIILEDQRTVVE
                     IKEIRLNDDEIKVKRRMDETGFASWGISKFGERIRYKIDRCKRQLETMTAKQYPGMLI
                     LYDTRPQPLCGISPTEIAVAMYGALTIEFRLPKEMGKPLVFERELFGEGKKLRYDTHN
                     YISALAVLHEQGIENTLHLDIYHNIYASRPLPLDRLVKRKDMTLFTIDGRKADQFQNW
                     QRITE"
     gene            17630..19321
                     /locus_tag="PKI64_02120"
     CDS             17630..19321
                     /locus_tag="PKI64_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOD99835.1"
                     /translation="MRRQTKWVLGVVLFTFIVPLLLCAAPPGMLNSGNAEISGKVTDL
                     KGTPLKDITVEVRQQSGQAWPVVKTNNTDAAGLYTVAGLGDGSYIVAFDDPAGVYARE
                     IYENTFLVYNAHTVTVTAASPATDINASLALAGKISGTFTNSAGSPLQFAYLVSFRKN
                     GVGWQPMESTLSDAAGNYALAGLPAGIYRIFYTYEGQEGYYDNAPDLLTATDISIAAG
                     QAVSNINFTVGERSNVGAISGNVTEEDGVTPLDHIYVTIFRWADIWWQRYTEGVTDAE
                     GKYLIENLPPGKYRVYFEASHYGEGKYYDNAPDIFSATDITVSAGETTTNINAAMSPG
                     AATVRGESGAIAGTVTQQDGTTPIAQVHVTAFRWVDVWWQRYAEGITDTEGNYSITNL
                     PPGTYRIQFEAWHGGFGEFYDNVPDIFHATDITVEEGQTTANINAALAGQGCPDYETW
                     SASIDWQGAPNGPADDADGDGFSNDLERIAGTNPVAANDFFTVSSAQLTPAGNGLILG
                     TALFGRAYTARYSTDIRLPWREWTPIELSFSDGLIIAPVIPNQPNVIYRLIVDLE"
     gene            complement(19714..21321)
                     /locus_tag="PKI64_02125"
     CDS             complement(19714..21321)
                     /locus_tag="PKI64_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD99836.1"
                     /translation="MRIEPYSPCPCGSGKKYKFCCYRKKPDTASFSATFHVAGEPVGT
                     APLPPSAADAGLRTAMDLNVKGNELLHRLELDEAIKIFQQAYAACADYTPALNNLALC
                     YFQKYEIEKAIETQQKALAVPRSNPFGQASLALYCRYLPHPKEQKQLAEDALRRALEM
                     HSVSFDSVLRTCKSLALYGWHQEILTLLETSDFGRNESLAFYGGVAAANLGEYERAAK
                     FLKIAQRAGEPANALAKRYRRMLRDHAVPNTIRGNWPYFTPDELIPLPVYGMSQQALR
                     DEVFTHSIAAVDLAESLINAGEDMADHGLTLLGESQHPAARELLEIIARSVTGPDARR
                     TRAAMLLKGDAPGSGKPQDLWFKGKQRSVITRRHTLNPDYRFCPDPPAKWAKLFKDSV
                     ELMQQNPANAPIAAEGFKKVFTNAPEVFPARFNYATTLLAMGRFAEAETILRDILREH
                     PTYLFAASALLRIMTRQGRMDEARDWLRTYEPPEETHPDAWLSWLFAQLAYHVKQGDR
                     DAVRPFRDMIMRLAPDHPLLDKPEHQL"
     gene            21510..22196
                     /locus_tag="PKI64_02130"
     CDS             21510..22196
                     /locus_tag="PKI64_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD99837.1"
                     /translation="MALGVWAVWIATAGWAAETPSFYAGISGGLAMPMNMLEEWSWPE
                     RGQQADLKEEIHNGFVIGAKAGWIPAVLGGRFAAELEYSYRRAEFDRIMSPGFAAGPY
                     TIAGFYSPATDSHIEFQSLFVNFLIRYPDGRVHPYIGFGPGVTRCTVSFNEAELTTEG
                     FGFAESGDDTTFCWQALAGVDFDISARVMVGAGYRYFAAQPSFTWANGTQSDYDPVYH
                     DVAVDLKFRF"
     gene            22219..23913
                     /locus_tag="PKI64_02135"
     CDS             22219..23913
                     /locus_tag="PKI64_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99838.1"
                     /translation="MQKKRMMGWVARIALVGLLAAGVASGQPEITAFNGNGQISWVDP
                     GGTGTHYAVQWSTSGLSNWMSWQDAEATLAGLGPTGSAAVPMFYRVVAQDPTYKPTES
                     YTYFQKLPLFDPLTPLATNEMRITFMGSMIPLPVRLAQAEMSIFVEVGWSNDPNDKVY
                     GGRARDQFIFDAGAGISANYAAAKVGFRRMDKVFINHLHGDHMNDLTHIYCFGPASDR
                     KSPLYVWGSKSSGVACPSNDWDIYNDDGVVYDDGTSNFCASLRSAWRWHSESFSFQGT
                     SYTNYVPPTKESWGLPHDPVPVGNDDPRDAYAVVPIELDWSVVGGVAYSNADTGATIT
                     HFPVIHCRRGSMGYKLDWLTPEGATLSMIYTSDTKPETNCIEQAKNGGNGVDVFIHEM
                     VVPPEVWAYKNMGLDAPPQPGDPNYAEFQATVQGLTFVQNSSHTPQGAFGYILSQINP
                     RPKLTVATHFPVADDTVASAFESVRAHCPDVEMGRDIVWSFDLMVLRVFPDHIEQARA
                     DVSRYSFNPPVQLPTGMRPPKYNDGMGKGDPFYQIDLSSEIPPVSGGVTNYRVDGY"
     gene            24063..25178
                     /locus_tag="PKI64_02140"
     CDS             24063..25178
                     /locus_tag="PKI64_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD99839.1"
                     /translation="MRYSILLSVIVLAVGCREKMPAPPPGPVPVVAADVIVRDQPIYY
                     EAMGQTLGSQDVEIRARVEGILESVHFREGAMVEKGALLYTIDPSTVEANLAQARGAL
                     AQAEAADDKARRDVRRLTPLWEKNAISRQMLDDALAAQHSASGAVVTARGAVDNMEIL
                     LGYTKIHAPIAGLIGKTEVKPGNLVGRGQNTLLTTLSVLDPIAVRFSISEKEFLTLQH
                     VIPEDQQALASAAIFELVLADGSSLPDKGKVVFVDRSVDPATGTMLLEVAFPNPGLLV
                     RPGQFGRVRAPIRTVAGAILVPQRAVQELQGTHSVFVVGEDGRAEFRPVRVGPRVDNL
                     YVIESGLHAGEKVVVEGGQKLQNGTAIALQPAAPASE"
     gene            25182..28301
                     /locus_tag="PKI64_02145"
     CDS             25182..28301
                     /locus_tag="PKI64_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOD99840.1"
                     /translation="MAQFFIRRPILAIVLAILMVLIGVQTLLTLPIEQYPQLSPPNIQ
                     VTAVYPGAGAEAVEQSVATPIEQQVNGVERMLYMKSLNSSDGRMQLSVTFDVGSDMDT
                     ANMLTQNRVAQAESRLPAEAVAQGLTVKKINPSILMVVSLYSPNGTYDSLFLNNYAVL
                     NVRDALLRVPGLSQVDMAGGAEYGMRIWLQPDKLAKLGLTPADVMSAIREQNAQAPAG
                     QVGAAPAPAGQEFTLTVRAPGRLTTPEEFGEILVRSTEDGRQVRLKDLARIELGAENY
                     KSFGRYNGREAGLLMLYLLPGANQIECAKEVYRTLDELKRYFPDDMDYAITYDSTPAV
                     SASIEEIVHTLFEAIVLVVLVVFIFLQNFRATLIPLAAIPVSLVGTFIFFPLMGFTIN
                     TLTMFGLVLAIGIVVDDAIVVVEAVMSHIERGKSPREATAQAMREVTGPVIGTSAIMV
                     AVFVPVAFIGGLTGRMYAPFALTIAVSVILSTFNALSLSPALASLMLRPAHIEQSPLR
                     WFYRGFNRVFTRTTGGYLRWATALGRRAAWSLLIIGGVALLGGGLSSVVPAGFVPIED
                     QGILLANLQLPNAASLERTSEAARQVEEVLRTTPGVESYNVIGGMSLLSGTYTPNTGT
                     FFIRLKPWAERTTPETSLIGLVLGLRQRLAAIPEAVAFPFVPPSLPGFGAAGGFDMLL
                     QDRSGNLSVEELHERAQAFLAAARQRPELTGLFSAFDPTVPQVELTVDREKARSLGVP
                     ISDVFATLQASLGGAYVNDFNRFGRLYRVFVQADSDFRRRPEDIGQFYVRSRTTNAMI
                     PLSTLVKVTPAAGAELTIRYNLMRSVQITGQAAEGYSSGQAMAALEAVARDVLPREMG
                     IAFTGLSYQEKNAPSSVPTMILAVVFVFLLLAALYESWALPWSVMLITPTVLLGAFLP
                     VWLLGFDNNIYVQIGLIMLIGLAAKNAILIVEFAKMQRDQGEAPLAAAMAAAKLRFRP
                     ILMTAFSFILGVLPLVLASGSGANARKMMGIAVLVGMLTATLLGVFLTPAFFIAIERL
                     SHRRQGGAA"
     gene            28298..29725
                     /locus_tag="PKI64_02150"
     CDS             28298..29725
                     /locus_tag="PKI64_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOD99841.1"
                     /translation="MIRHRHAALILLPLLLAGCATGPDYQRPDLALPEAWRGGPATSE
                     SLADLAYADFYRDPVLVALITTALTNSPDVRAAAARIEQAAAALRAQRADYWPAVNGT
                     ANYTRARKGNLPPVPGVEGDQFDVFGVLSYEVDVWGRIRRLNEAARAQYLATAEARRT
                     VEIGLVAGVASAYFNLRALDSQLAIARATLASRRDMLELTQIKFDDGNGIVSELDVAQ
                     AQTQVAAAQSAMANLQRAIAATENALNWLLGGYPGDIPRGLDIVQQWQPEDVPAGLPA
                     DLLLRRPDVCAAEQKLIAANANIGVARAAYFPALSLTGALGLQSDDLADLFDTGLAKA
                     WTFSPNLAAPIFNAGKIRAGVRVAAAQRQEALANYEKAVQTAFREVADALTGQEYLRQ
                     QLAADEETLRAETRRLDLAWARYEGGVASYSDVLDAQRYQFSAELTAVQTRNELLQSL
                     VQLYKALGGGWGSSAASPTQAKGNP"
     gene            29722..31113
                     /locus_tag="PKI64_02155"
     CDS             29722..31113
                     /locus_tag="PKI64_02155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD99842.1"
                     /translation="MKIYIAGAAGGEVTGSAFYVQTDQACVLVDCGQFQGSRQAEALN
                     RWRPPARIKLNAVLITHAHLDHTGRLPLVAQKGHKVPVYATEATLELTALILRDSAHI
                     LAADAMHKSRRLARAGKPPVTPLYSLEDVENALRMMRPVPYQEPVQVAPGIQAIWTEA
                     GHMLGSASIQLIVEEGGRQKRIVFSGDLGPRSAPILRSFEPFHEADMVFLESTYGSRD
                     HRPFVNTVEEFVGIVNEAIAAGGKMLVPTFAVGRAQLIAGLLGWMFRNQKAQPFPIYM
                     DSPMAIQASAIYTRHHELFDDATLKFIEQGTLRDELRTMKLCVTADDSRQINQAAGPC
                     LVMAGAGMCNAGRILHHFKANLWKPETHVLIVGYQAAGTLGRQLVDGAEYVKIFGEKI
                     AVKANVHTMGGFSAHAGQTDLLAWLEAIAPSRPRVVLVHGETESRQGLAVQIQRRFDL
                     PAELPAMNEVLEL"
     gene            31144..31887
                     /locus_tag="PKI64_02160"
     CDS             31144..31887
                     /locus_tag="PKI64_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99843.1"
                     /translation="MNKKIFWGLTAGLTLVVMVAVAEPVPPTFEFVPSLWVAGLSGSG
                     TLHGQDVKYDRSTGDLWEHLEAGGSLAGKVVWNGWALGGQLDGYQLSTDALTVNGTPL
                     AGQMDTDLIFGELAAGRVFPGWKEGQTFTLALGFRYARIANDLDAHGTGSFHHRQDRW
                     DPMIILWPSLPLLASKVQGLSLTPAFSIGGGGDSKLVYELFPQVRYQVTPHLVARAGY
                     RAIGYRAKHSADELKFTLAGFIVGLGVTF"
     gene            31949..32194
                     /locus_tag="PKI64_02165"
     CDS             31949..32194
                     /locus_tag="PKI64_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99844.1"
                     /translation="MRDFPVEVVACFVGLVLIALFAHILFRKLRFPCTVGLVFVGMLL
                     FKLGGVFEPLNVLQRVHLTPNFIPKNAFGHRGCVITR"
BASE COUNT         6130 a         9426 c        10364 g         6281 t
ORIGIN      
        1 gtgaaaaaaa caaaaatatg attgacggac atcctcagag gtctgcgttc ctgcttggtt
       61 cggtggctgc cggttcggtg gtcttcacct gaactctgcc ctctgccctc tgaactcttc
      121 tcgccctcac ctgccctctg aaccctgaac cctgatccct cgccgtgttg cggcggcgcg
      181 ggtaaaacta gggttgcgtc cgcggcccat ttttgagtga atgcgcggcc aaatgtgcgg
      241 gccgcaaggt gcggcggcac gaggagtttt tgtgtatctc aaagcagtcg aaatgatcgg
      301 gttcaagagc ttcgccgagc ggacgaagat gatgtttgaa cccgggatga tggcaatcgt
      361 gggccccaac ggctgcggca agagcaatgt gtcggacgcc atccgctggg tgctgggcga
      421 gcaaagcccc aaggcgctac gcggtggcac gatgaccgat gtgatcttca acggcaccga
      481 ccaggccaag ccgctggcca tggccgaggt cagcctgacc ctgaccgatt gcgaggcggt
      541 gctgggcgtg gagtataacg aggtgacggt gacgcgccgc gtgttccgct ctggcgaagg
      601 acagtatttt atcaataaga cgccgtgccg cctgaaggac atccagcggc tgttcatgga
      661 taccgggctg ggcactgatt cgtatagcgt gatggagcag gggcgcattg accagattct
      721 cagctcgcgc ccggatgacc gccgcgaggt gttcgaagag gccagcggca tcagcaagta
      781 caaggccgac aagcgcgaag cgctgcgcaa gttggaacac accgaggcca acctgctgcg
      841 cctgagcgac atcatcaagg aagtgaagcg ccagatcatc tcgctgcaac ggcaggtggg
      901 caaggcgcgg cgctacaaaa atttgcagga gcaactgcgc gccagcgatg tctggctggc
      961 caagggccgc ctgagcgagc tggacctcgc cctcgccacg ctggagaagg agctgggcca
     1021 gcaccaagcc accgaagacg gcctgcgcca aaaggtggag ggcgccgagg ctgcggcggg
     1081 cgcgttgcgc gccaatatgg cggcagtgga aggccggatt gccgccgcgc tggatgcgtc
     1141 gtccgaagcc agcggcgcgc tggctcgcac gcaggagttg atccgggtca atggtgaacg
     1201 cgcgcaggaa ctcgagacgc tttcccagcg cgactcgcgc gagtccgcca gcgcctccgc
     1261 cagtttggcc agccatcagg ccgagctgga cgtgctgaaa aaagatttga ttgccgccac
     1321 cgatgcccac gaaaccgccc gccttgaatt gaccaaacgg caggccgcgc tgaccgccca
     1381 cgaccagcaa accgatgccg cccgcaagcg gttgcaggat ttgcgcacgg aggcgctgga
     1441 gctggagtcg cgcttggctc ggctgcaaaa cgaatggaac gaaatcgaag cccgcgagcg
     1501 gacggatctg atccgccgcg agcgctgcgc cgccgaaaag gccgaggccg agcgcgccgt
     1561 ggagtcctac gccgagcgcc tcgccctgat ggctgctcaa cgcgcacaaa aacaaaccgc
     1621 cgcggagcag gcccagcaac aggtggcggc gctgcgcgcg tcgcaggcgg accgcgccac
     1681 tcagttggcc caggtgcagc aggagctgtc gcggctggct tccgccagcg ccgcgcgcga
     1741 ggcgcaagtg gccctgttga cgcgcagccg cgaagaggcg cgcggcttcc ccgccggcgc
     1801 caagaagctg ctcgaaggcg ccacagtgcc gggcgtgaat cccaaagaca tcctgggcgc
     1861 gctggcccaa aaagtgcgcg cgcccaaaga ggatcaactg gcgctcgagg cggtgttgcg
     1921 ctcgtggctg gatgccgtac tggtgcgcga tgccgccgcg gccgtagcca ttgcccgcca
     1981 agtgcaggcc gccgccggcg gtgcggtgcg gctgctggcg ctggaagccg gcgaagttcc
     2041 cgatactccg ccgggcgcgg gcgaaccgct cctgccgcgc gtgaaggccg cggccgatct
     2101 ggcggggctc ctgcgccggt tgctcggcgc ggtgcgcgtg gtgcccaccg ccgccgatat
     2161 gccggcggca atccccgcgg ggttgaccta tgtcacgcgc gatggcctcg tggcccgcgg
     2221 cgatggcagc gttgagttct gggcccccgg cgaaaacgaa agcaacccgc ttgcccgcga
     2281 gcaccaactg gcggaatggc aggaagattt ggctcgccta cggcaggagg tcgaaaacca
     2341 taacgcgcag gccgcggcgc tccagcagga tgagcaggcc gcgcgcgagt ccatggccgc
     2401 cgcacaaacc gcgtgggaag cccagcagcg cgagatggca ctggccgaag gcgaaatccg
     2461 catggtcgag cgtgaggcca aacaggccgg cgaacgcgcc gaaaccatcg gctgggaatt
     2521 ggccgcgctg ctgaaaaaga ccgatgccgg cacccagcgt cgcagcgaaa tggcgggcga
     2581 gatggaggac atccgccagc ggcaggccgc cgtgcgcacg cagaccaccg ccgattccga
     2641 aaccctccgc aaactggaag atgaacgcaa cgcgcggctg gaagaagcca ccgaatgtcg
     2701 cgtggcgctg gccggctata aacagcaggt ggatggcctg cgcgcccgcg gcgagccgct
     2761 ggaagcgcgc ctgcgcgagc tgaccgcctt gatcagcgag cgtgccgccg gcattgattc
     2821 ctaccgcgag cgcatcgccg cgctggccgc cgccaccgcc aacgcgcagc agcaactggc
     2881 gccgctcaac gacgaagtgg cccgctgcca ggccgagctc caggaagctc gcgccctgcg
     2941 cgaccgccaa aatgccgagc tgacccaaag cgatgccgcc ctccattccc tgcgcgccga
     3001 gttggatacc atccgcgaac agcgcaacac catggaagtc caattggccc gccaccgcct
     3061 gcaccgccag aaccttgtgg aacgcatgag cgccgaatac agcctgcaac ccgccgagat
     3121 tctcaaggcc aaggaaccgg agtgggaaga ggatgccaag cccgccgacc gcgaagcgct
     3181 tgaaaccatg gttgcggaaa tccgtaccaa gctccaaagc atggggcccg tcaatctggt
     3241 ggccatcgag gaacatcagg agttagaaga acgctacgcc ttcctgaacc agcagaacga
     3301 tgacctgctc agcgccaagc agcagttgct ggacctgatc cgcaagatca acgaaaccac
     3361 caccgtgctc ttcaccgaca ccttcaacaa ggtgaacgac aacttccagg agctgttcaa
     3421 gcagctcttt ggcggcggca acgccaagct ggtgctggtg gatgaagaaa atgtgctgga
     3481 gtccggcatc gagattattg cccgcccgcc cggcaaaaaa ttgcagacca tttcgctgct
     3541 ctccggcggc gagcgcacat tgactgccgt ggccttgctt ttcgcgctat tcaaggtcaa
     3601 acccagcgcc ttctgcctta ccgacgagtt ggatgccgcg ctcgacgaca gcaacatctc
     3661 ccgttatctg gaaatgctca aggggttcct caaggacacc caattcattg tcatcacgca
     3721 caaccggcaa actatcggct cggccgacgc cctctacggc gtgaccatgg aaaagcaagg
     3781 catctccaag gtggtgtccg tgaagttccg cggcaaggaa aagccgcctg cggctgacga
     3841 tacccccgcg taaggatgcc cggcgcggtc tggtgggcgg tgagggattt gaacccccga
     3901 cattcacggt gtaaacgtga cgctctgacc aactgagcta accgcccaaa accgtgcaca
     3961 ccctacggca ccccgttccc cagcgcaatc ccgttatcat ccggcctccg ccataatact
     4021 ccactcacga gcgcgttcgc cagcttgacg tgaaaccggg cctatgttga taatgacaat
     4081 atggcaaggg ggcgcaaaca gggtgtgccg ggcgcggttc gtcgcgtgat gaaccggagc
     4141 catcggcaat ttactttgcc caatgcgggc gaggggacgt ggggggaaat gttgggacag
     4201 gtaaaacaaa acgcatcagg gaggatcaac gcatgaaacg gagtctctat ccttattcgg
     4261 gccggcagac gattctgggc atccaaatgc tgtttgtggc ttttggcgcg ctggtgctgg
     4321 tgccgttgct gacgggcttg gatccgtcca tcgccctgtt cacggcaggc gtcggcacgc
     4381 tgctgttcca cgccatcacc ggcggttccg tgccgatctt tctggccagt tcgttcgctt
     4441 tcattgcccc cattcaattc gccatgaaag aattcggcac tggcgccacg ctgggcgcga
     4501 ttttctttgc cgggttggtc tatctgatct tcagcttgat ggcgcggatc ggtggccgcg
     4561 aaatgctcaa tcgctatctg ccgcctatcg tcaccggccc cgtcatcatg gtgatcggct
     4621 tgaaactcgc gcccgtggcg gtgggcatga cgcgcaattt cgacggctcc gccgcgcttg
     4681 atggtcgggc gctgttcttg gccacctgct cattgctgac ggctgtggcc gcggtgatgt
     4741 tcggcaaaaa gctgttgagc ctgattccca ttctgtgcgg gattgtcgtt ggctatctcg
     4801 tggccatcgc catgggcgtg gtggattttt ccatcattcg cgaagcgtct tggttccaac
     4861 tgccatggac gctcgcgcgc gaaaacggct cgttcgccat gccgcgcttt gatcccgcag
     4921 ccatcctcta tatgctgccg gttgcgctgg cgccagccat cgagcacatc ggtgatatcc
     4981 tggccatttc ttctgtcacc ggccggaact atctcaccaa tcccggcctg caccgcaccc
     5041 tgctgggcga tggcctcgcc acctcgctgg cgggcctgct cggcgggccg cccaacacca
     5101 cctattcgga agtcaccggc gccgtggcgc tcaccaaggc ctatgatccg gtgctgatgc
     5161 gcattgccgc cgtggtggcc atgatcctcg cgtttgcggg caaactcggc gccatcctac
     5221 gtaccatccc cgggcccgtg atgggcggca tcatggtgct gctcttcggg atgattgccg
     5281 ccatcggcat caactccctc gtcaaggcgc gcgtggatat gtccaaatcc cgaaaccttg
     5341 tgattgcttc cgtcatcttg acctccggca tcggcgacat gacctttacc tgcgggcgct
     5401 tctgcttgag cggcatcggc ctcgccggcc tcgtcggcgt gctgctcaat ctcctcctcc
     5461 ccggcccccg cgaggcccac gaagagcagc ctgaaaatat ttaacaggct aaacacgccc
     5521 tccaaaaaac agggcaacag tttctcttcc atggcatggg gtgcgcgagt ccgggcacag
     5581 gttatcgggt tgtcagcggg ttgatgccgg gggtaggatg cccgccatga aacgggtgag
     5641 cgtggcgacg gggttggatt accgccagcg agtgtgccgg gcgatggatt atatccatgc
     5701 ccacctgaac cgcgagctgt cgctggcgga aatcgcgcag gcggccagct tctccgcgtt
     5761 tcattttcac cggatttttt ctgccgtgac gggggaaacc gtattcgggt ttatgcggcg
     5821 cctgcgcttg gagcgggcgg ccaatcagtt gttggggcat ccgcaaagcg acatcacgag
     5881 cattgcgctg gccaacggat tttccagttc gcagaatttt gccaaggcct ttcgggcgca
     5941 ttttggctgc gctccatccg cgtttcgccg gcgcaaggat ggacacacaa agcgcaagga
     6001 gcgagaagtc ttctcgctgc gtttggcgca ggatgcgcgg caggtgaaga ggacttcacc
     6061 acaacccgaa aggaaatgga cgatgcaaaa agtagaagtg aaaaccatgc cggcgtggca
     6121 tgtggcgtat gtgcggcggt tggggccgta tggcaaagca acctgcgagg ccgcattcca
     6181 agagctgatg cgctgggcag ggccgcggca gttgctgggc aaggggccga tgctgagcat
     6241 ctactgggat aatcccgagg tgactgcgcc ggccaactgc cgggtggatg cttgcttggt
     6301 ggtgcccgct ggtacgccgg tcgaaccgcc ggtggcgctc caagatgtgc tgggtgggcc
     6361 gtatctggtg tgtgcattca acctgccgcc gaccgacttc accgccgcgt ggaccgaggc
     6421 gtttcaatac ttggtccaaa aaggttatga atgcgcggac cggccctgct atgagttgta
     6481 ttacgatgat tgtaccggcg cgacatgccg gttcgatatc tgtatcccac tcctccaaaa
     6541 gtaagcagcg ccatactccg gcggcccggc ttttccgggc cgcctttttc ctcttccgag
     6601 gcaatcactg cgcgccaaat aaacgatggg cagaatgtca attgttaagg gctgacccca
     6661 aacggttaga gggtgccgag ggaaaggtcg ccgtggattt tgtagatggg gccgtcgcgg
     6721 tccacgtgca gagtgtggcc cggaaatgcg gcgggaagtt tttcccggag gcggcgggcc
     6781 accatgtcct ccagctcttg ctgaatggcc ggtgggatgg ccgtcagggt ggcgatgcgc
     6841 gcttggacca catagccggg gccatactgc gaaccgatgc cgactgtgaa cgcggcgcca
     6901 aggtcgaaga gtccatcgtg ttctcggttc gcggtggcgc catggggcgg gcggttggga
     6961 tgccgccggg cgcggtggcc ataacggagt tcgagttcgg tatcaatttc gtcgaacacc
     7021 tttttgagcc gggcttccca gcgttccaaa cgggttttgc cggcgctcat ggggcgggca
     7081 ggagcgtgcc ggggttgagc agcccggtgg ggtcaaggtg ctgtttcagc ttgcgcatat
     7141 cggcgatggc tttctcgccg gccatctggc gaaagaggtc acgcttgagt ttgccaatgc
     7201 cgtgctcggc ggaaatgctg ccgccccacg cgatgatttg cgcggcccag cgggcataca
     7261 gttcgcggcc ggcggcgtag tcgtcggcat tacggggcag gatgttgacg tgcaggtggt
     7321 tggcgccgat gtggccgaag atgaggtggt cgaggcccgt ggcggctaag tcgcgttcat
     7381 agagcgcgca gacatcggcg aggtggtcat cgggcacgga catgtcggtg ccaagtttga
     7441 tgatgccggg gtgttggcgg cggcgttcat caatctcggc gttgacgagt tccgggacag
     7501 cgtggcggaa gagtttgagt ttttccagtt cgtgcgggtt atcggccagg atgcaggcat
     7561 cggggttggc gccgaggtcg ggaagctgtt cggcggcggc cagcatggcg gcttcggcgg
     7621 cagcggcgcc cggggcatgc cactcggcat agacgcacgc ggcgcccggc ggcagggccg
     7681 gcactgcaat gcccttcgag gccagaatgg ccgcgcggtc ttgcaggaaa cgcaagcagc
     7741 gggaatcaaa atattccagc gcggccagcg gcgcggggct ggccgtgcgg ccggcccgca
     7801 aggcgttgac caagtccacc acggctgcgg tggaagggag gaacatcatc aggcccacga
     7861 tggcggcggg cgcggggagc aggcgcaggg tggcggcggt cacgatgccc aaagtgcctt
     7921 ccgcgccgat aaataaatcg cacaggtcca tgtcgggggc ggtgaagtag ccggcggcat
     7981 ttttgacggc cggctgcggc agcgcaggga gttcgccctg ccacgcgccg agagtggcgg
     8041 cgcggccgcg ggcttggtcg cgcccgcggc gcaggtcaag gacttcgcca ctagccagcg
     8101 cgacgcgtag gcctaggacg tggttgcgcg tggggccatg gagaaaggag agcgcgccgg
     8161 acgagttgca ggcaatcatg ccgccgatgc tggcggtggt ttcggtggga tcgggcgcga
     8221 agaaatagcc ggggggcgtg gcggcgcgga tggcgttgag ggtggcgccc ggctcaacgc
     8281 gcagcgtgtc gccggcagcc tcgatggcgg tcaggcgcga cagattcagg atgagcccac
     8341 cctccggaac ggcgccgccg gctacgccgg tgcgcgcgcc ttggacagtg acgggccagc
     8401 ggttggcgcg ggcttcggcc aaggcggcca gtacgtcctc ttcgctgcgg ggaaagacga
     8461 tttgttcggc gtggccggtg cggcgcgatt cgtcgcgcag ataatcgcca tgactgggtt
     8521 gaagcggttc actcatgcgc ccaatataac ttgcttcacg tcttgctgca cagataaact
     8581 acccgattca agtaatagga gaaagcaatg accaaacgga tggttaaagt aaagttcgag
     8641 catgggcccg aacaagatgt ggaagccgga attgcgttga gccagatttt ggcccagttc
     8701 ccggcggtga aggaaggcct ggagtttatc ccgctgggcg cgttggtgaa caacgagttt
     8761 gtctcggtgg aataccggat tgaaaccgac agcacggtgc ggttgctggg ctaccgggat
     8821 tcctatgggc agcggattta ccgctacacg atcgcctttt tgctggcgaa ggtggcgcat
     8881 gagctgttcc cgggctgtga tttcgcggtg gagcactcgc tctccaaggg gctatattgc
     8941 agcttccggc tgggcgatgt gccgggcatc agcgctgaac aactggccaa gctggatgcc
     9001 gccctgcgca aactgctgga tgacaaggcg ccgattgacc ggatcaagat cacctacgac
     9061 gaggccatcg cccactttga ggcggccggc gcgaaggaca agctcaacct gctgcggttc
     9121 aaaaacagct ccaaggtcac ggtgtatcag tgcggccggt tcatggactt ggccaaccag
     9181 ccgctggcca acaacgccgc ggcactggtg aactaccagt tgatcccgta tcagcagggt
     9241 ttcgtgatga tgggcccgga ccggatggac ccgacaacct tcccgccgtt tgagcctgcg
     9301 cactacattt atgacgtttt caagggccac aaggactggg gccggattgt gcgcgtgcgt
     9361 acggtgggcg acctgaacga acgcatcgcc cgcaagaaga ttgacgactt cattgacgtg
     9421 aacgaggcct atcaggaaaa gcgcattgcg ctactggcgg agcagattgt gcaacgccgc
     9481 gggcaggtga agtggattct gattgccggg ccgtcatcgt cgggcaagac cacgttctcc
     9541 aagcgcctct cgctgcaatt gaaggccagc ggcatgaacg ccaagccaat ctcgctggac
     9601 aactacttcg tgggccgcga caagacgccg gtggacgaaa acggcgaggt ggacttcgag
     9661 cacatcgagg cgttggactt ggatctggtc cacgagcaac tggccaagct ggattccggc
     9721 gagcggctgg aactgccgta tttcaacttc cacaccggcc agcgcgagtg gaagggcgaa
     9781 atgatgcaca tcggcccgga tgacgtggtg gtgctggagg gcatccacgg actgaacccg
     9841 cggctgactt cctccatccc cgtggagcac aagttccgca tctacatcag cgcgttgacg
     9901 cagttgaaca ttgacagcaa caaccgcatt tccaccacgg acaaccggtt gatccgccgc
     9961 atggtgcgcg acaacaattt ccgcagccac aacgcgctgc gcacgctgca aatgtggccc
    10021 aaggtccgcc gcggcgaaaa aacctggatt ttcccattcc agaaggaagc cgacgtggcc
    10081 ttcaactccg cgctggacta cgagctggcc gtgctgcggc cgttcgcgga acccctgctg
    10141 gcggagatca agccgtccat gccggaatat gcggaggcgg tgcgcatcca gcagttcctg
    10201 tcgaacttcc tcgtgatttc gccggaccgg gtgccgccca ccagcgtgat ccgcgaattc
    10261 atcggcaaga gcttcttcca gtactaaacg cgaaccggaa aaccccgcca agggcgcttg
    10321 gcccgcaccc gccggcaacc aaccggcgtt tgcgctgccg gcgtcctttg cgttatcttg
    10381 gctgccatga ccacaccgac tccaaccaac tcgcccgccc ggatggtcgc cattgtgggc
    10441 cggcccaacg tgggcaagtc ggcgctgttc aaccgcctcg tgcggcggcg gctggccatt
    10501 gtgcattccg aagaaggcgt gacccgcgac cgcctgctgg cgcgggcgac gtggctgggc
    10561 cagacctttc aggtggtgga taccggcggg ctggccttga tggatcgcgc caagacgggc
    10621 gacgacatcc aacgcgccac ggcggagcaa gtcatggccg cgctggccga ttccgccgcg
    10681 gcgattctgg tggtggacat caccgcgggc atccagccgt tggatgtgga agtggcccgc
    10741 ctgctccggc aggccgggcg gccggtggtg ttggccgcca acaaggcgga cttgccggaa
    10801 tgggatgacc acaccgacga tttcgcgcgg ctgggattcc cggtggtggc gatttccgcc
    10861 gcgcacaatc gcggcatcga ggcgctgctg gaagaggtcc tgccgcacct gccgccggtc
    10921 ggtggcgaag ccgagccccc gcgcctgaag gtggcggtgg tggggcggcc gaatgtcggc
    10981 aaatcatcct acatcaacaa actgaccggc cagcaacggg tgattgtgtc cgacattccc
    11041 ggcaccacgc gcgacacgat tgacgtgccg ttcacgctgc gcaccgcaac cggcgagcag
    11101 cactacacgc tggtggacac cgccggcatg cgccgccggg ggcgggtgca tcaatcggtc
    11161 gaaaagttca gcctcttccg cacggaacaa tgcattgccg aggccgacat tgtggtgttg
    11221 atgctggatg ccgccgaggg gcccggcgcg caggaaaaga aaatcgcggc gatgatccgc
    11281 gagcatcacc gcgcctgcgt ggtgctggtg aacaagtggg acttggccac gggctccggc
    11341 gtcaaggccg aggactacgc ggcacggatg cgcgccgatc tgttttttct ggatgacatt
    11401 cccattttgt ttgtgtcggc ggccagcggc taccacattc gccggagttt tgaggtcatt
    11461 gccacggtgg ccgcgcggct gtccaccaaa atgcccacgg gcgtgttgaa tcgcgtgcta
    11521 cgcgacgcgt tcatgcgctc gcaaccgccc ttcacccgcg gccggcaact gaagttttat
    11581 tacgccacgc aaaccgggct gaacccgccg cgcgtcacgt tgtttgtcaa cgatcccacg
    11641 ctgaacaccc ccacgcacca agcctatttg accgcacgcc tgcgcgcggc gttcgatttg
    11701 gccggcgtgc cgctggtcct gcgctaccgc tccagccacg gcgcgcccga ggaacctacc
    11761 tgagccgggc cgcgcgcccc ttggagagcc gccatctttt cgcgtccgtc caactggaag
    11821 cccggccagc catatcccgg cggctaccgc gaggtgtggc gcgtcgcggc gccgatcatc
    11881 atctcgatgg cgtcgttcac cctgatgcag ttttgcgacc gcatcttttt ggcgcgctac
    11941 agttcggtca cactgcgcgc cgcgttgccg ggcggcattt tggcgttcac gctcacggct
    12001 tttttccagg cgctggccgg ctacgcgggc acgtttgtgg cgcaatatca tggcgcgggc
    12061 cgccgcgagg aatgcggccg cgccacggcg caaggcattt ggctggggct gttgacctgg
    12121 ccgctgtgta tcgcgctgat tccgtttggc gattggctgc tgtccgtggc ggggcacccg
    12181 gcggatgtgc tggcggcgga gcaaacctac ttgggtattt tgatgcttgg cggcggaatt
    12241 catgccatta cgaacgcgct ggcgggtttc ttttcggggc ggggcgacac ccgcacgccg
    12301 atgctcgcca gcctgttggc caacctcgcc aacatcgggc tggattacgc gatgattttt
    12361 ggccactggg gttttccgga gtggggcatc gcggggggcg ccatcgccac agtgatcgcg
    12421 tcggcgctcg gcatggcgat gctgctggtg ctgtattatc aggaaaggtt ccggcgcgaa
    12481 tatggcacat ggcgtggacg gcggttggat tggcagcgca tgggcccgct gctgcgcttc
    12541 ggcgttccgg cggccatcaa tacggtgcag gatgtgggcg catttgcgtt ttttgtggta
    12601 ttgctgggtc gcctgccgcc ggtggaaatg gccgccagca atatcgcgtt cagcatcaat
    12661 aatgtggcgt tcatgccgct gctgggcatg gggatggcgg ccacgattgt ggtggggcag
    12721 caccagggcg cgcgcaaccc cgccaccgcg gcacgggcgg ggtggacggc attgaagctg
    12781 tcgtggttct acatgggcgc catcgcgctg accttcctgc tgctgccgcg gatgtatttt
    12841 gcgctgttca ccggcgaagg cccggatgtg ctgcacatgg atgaggtgat cggcctcggc
    12901 cgctggttgt tggtgctgat ggcgctgtgg ggaatgctgg acgccatcaa cctcgtggtg
    12961 gccggctcac tcaagggcgc gggcgatacc cggtttgtgc tgctgtattc cgccgttgtg
    13021 acgtgggggg tgtggattcc cggcgagctg gtgctggtgt tgtggtgcca cgccgggctg
    13081 ctggccacct ggctctggat gtcggtgtat gtctttctgc tggccggtgg cctgtggctg
    13141 cgcttcttgc gcggcaaatg gaaacacatt gacatgatcc gccagccgca ccccgagctg
    13201 ccggcggaat aactttttga aaatatattt accgcggggc ggggcggggt gtaggttttc
    13261 gtggagaaag aaacggggcc gaacgtgtgg gatgacgcca tcgcgcagga tgtgaacgcc
    13321 gggattcggg cctggcgcgc gcgcgttttg caagtgctgc tgatctttag ttccgtgctg
    13381 ctgttcgtgc cgctcgtggc ggtttgggcg gggtggggga tggaactgac ccggccgctg
    13441 cgggttctgt ttgcgctggt ttacctgctg ctggtggtta cggcgctctg gcggcgcggg
    13501 ccttttgcgt ggcggctggg ggtgcttttg ggcctgctgg cgctggtggg atttttgcgc
    13561 ttgttgatcg gccggttgga cggcagcgga cggatcaccc tgctgctgtt gccactgttg
    13621 gcgctggtgc tggcggggcc tcgcgtgggg tgggccatgg ttggcgtggc ggtggcggaa
    13681 tatgtggccg taccgctgct gttgcaatcc ggctggctgg cgggacgggg cttggcgtgg
    13741 accgccaccg accgctcgcc ggccttttgg gggttgcagg gaatcttcgg cttggtggtg
    13801 ctgctgacgt tgatgttcct ctttacccgt tttcatgcgc ggctttttgg cgccatgcgc
    13861 gccgaatacc aggctcggcg ccagttagag gtggaaaccg ccgaccgcca gcgactcgaa
    13921 gccgccatcg tgcgcgtgga tgaagaagaa cgccgccgcc tcggctcgga tttgcatgac
    13981 ggcctttgcc agcatttgac cgccacgctg ctcaactgtt cgctcttgga aaaacgctgg
    14041 gccgaggctg gccagccgga tgccgtggcg ctgggccaag tgcgccaaag cattgcggac
    14101 tccatcggca tggcccacga tgtggcccaa gggctctgcc ccatggacct cgcgcccgac
    14161 gccctgctgc ctgcgttgga acatctctgc cgcgaaactc gcgaacacca cggcatccag
    14221 tgccggttgc aggcgccgac ggcggtggcc atccgccggc cggaggatgc catgcactgg
    14281 tatcgcatcg cgcgcgaagc ggtggccaac gccatcaaac acgcccgctg cacgcgcctt
    14341 gatctcacgc tggaacacga tggcgacgac ctcgtgctgc gcatttgcga tgacggcccc
    14401 cacacgccgc cggataccgt cccagtagcc ggcctcgggc tcaatattct gaaataccgc
    14461 gcgcaattgc tgggcggggt cttgaccgtg gccggcgggc ccgccggcgg aatgtgcgtg
    14521 acctgccgcg cgccgcaggg aggaaaatcg gcatgaccaa ggcggaaccc atccggattt
    14581 ttctggtgga tgaccatccg ctggtgcgcg ccgcgttgga gcaactgctc gcgggccacg
    14641 gccacgcggt cagcggccaa gccgggagcc ccgcggaagt gttggctcac ccggcgctga
    14701 cccgcagcca cttggtggtg gtggacctgg cgctggacaa ggaaaacggc atgagcctga
    14761 tccctgcgct gcgcgcgcgg cgcacgccgg tgctggtgta ttcgatgcac gaaagcgccc
    14821 acatcatccg gcaggcgctg gccgccggtg cgttgggtta tgtcaccaag cgcgaagcgg
    14881 cggattcgct cctggcggcc atccacgctg tgcggcaagg agaaacctat ttgagtccgc
    14941 gcgccgctgc cgcgttgcgc acggaaagcc cgctggctgg cttgagcggt cagcaattgc
    15001 aaatctaccg gcggttgggg caggggttgg ccaacgagga aatcgcccga cagttgggca
    15061 tcagcgtccg cacattggaa tcctactgcg tgcgcatcat ggataaactc ggggtgcagg
    15121 gcgccaagga acttcgccag cacgccatcc gcgccgccat cgccaccggc ccggatcagc
    15181 ccgaaccgta accaatacca tcattcaaaa atcggggtcc tgtggagtgg ggtatatggg
    15241 ggcggtttac cacgtgatat accaatggaa ccgctgcagg gatgtttcgc tcccctttgc
    15301 atagcgggat ccgattgcaa tcggatgatc aaaaacagcc cggagaaata cttcgtgatc
    15361 tagcccgtgc agcggcatac aggtgtcggc aatggataga tagtgatggg cgacccattt
    15421 gcgttttcat aaagccgccc agacaacccc aatttctccg ttgccggcaa aatgctttcc
    15481 cgttatttta ggatcaaaca gttgggatat tccagccttt cttgggtaga caaaactgtt
    15541 ggcaaaacag acgtgtatta atcggccgtg catgttggtg tcacaaatga gatacaaaga
    15601 taagacaatg tgggacacca ttaaactgac aaggattcag agcctgcccg gtaaaaagtt
    15661 ctggaaatcg ctatcgccca catttcttca ctttggcgag caggaagaat tatgtgcagc
    15721 tcatgaagca gtgctgaaat ctgaatttca gcacagttgg caacaggcta ccgaaagtat
    15781 tatctgtgga ttagctggta gagatcagtg caaaaaatac acacgttcac gtctaaaaaa
    15841 acttggtaga cgcaggaaag acaaggccca gtcatgggag tatattttac cacaatcggt
    15901 acttcttcat gcgtggctag ccttccgaat ggccgaagct gctgcaaaac aaacatacac
    15961 gtccaatcct atccagattg atggtggaga taaccatcaa atgctcaacc tagtgtctca
    16021 acgttatacg cccgagaaca ggtctaatgt tgatgcatgt ctcgacaatg actcaaaagc
    16081 aattggacta tggatggtcg attatgttcg aacggttcta atcccacgga ttaacgaact
    16141 tgctagaaga tatttcggca aagctcatgc tgatattccc gaatattttc cactcaaata
    16201 taaaatttat tatacggaac caagtaacta ctggcataac cctgataaaa aagggtgtat
    16261 acgcttattg gaaagttggc tgtcaaaaac aatccgtggt cccagctctg acttggaatg
    16321 tgtgagtgca atcgatgtat ttctgaatta cgtttcaagc gttaatcatg tgtattcaca
    16381 cttcacattc gttaaagaac tgtatgcatt ttttaatgag gattcagaga tatatcgtac
    16441 tcttgagaat ggagtttgtc ctggtgcatt ccatgccgtt aggcaacgta ttctaaacat
    16501 atccataggt gatatatcaa aacccctctt tcttcggcaa aaactctccc ccccccgcaa
    16561 gtagtattcg tatgatccca acgccttatt taggggagta ttagaccacc aaggtactcc
    16621 agtactacgc gccacaagca gcgtgagggt gaacgctgac atccatcagt aacgatgacc
    16681 gaatctgaag ctaaatttga ggcctattgc agtgttaatg caattccgta tacccgaata
    16741 gcagtaggag aagacaagac acctgactat gagattattc ttgaggatca gcgtactgtc
    16801 gtggaaatca aagaaatacg actcaacgat gatgaaataa aggttaaacg caggatggat
    16861 gaaaccggct tcgcgtcttg gggtataagt aaatttggtg agagaattcg ctataagatt
    16921 gatcggtgta aacggcagtt ggaaactatg accgctaagc aatacccggg catgctgatt
    16981 ctttatgaca ctagacccca accattatgc ggaatatctc ccactgaaat cgcggttgcc
    17041 atgtatggag cacttactat tgaattcagg ttgcccaaag aaatgggtaa gccactcgtt
    17101 ttcgagcggg agttatttgg tgaggggaag aagctgcgct atgatacgca taattatata
    17161 agcgcacttg cggtcctcca tgaacagggg atcgagaaca cgcttcacct cgacatttat
    17221 cacaatatct atgcttcgcg accgctccct ttggaccggc tcgtaaagcg aaaagatatg
    17281 acactgttca caatcgatgg gcggaaagct gatcaattcc agaattggca gcgtattaca
    17341 gaatgaaaac agggcgttcc gctgagatga tgttttgaat ttgggcggat agagggttgt
    17401 ggagtgaacg ctctatgcaa agtaatccag agtggtatat gcggggtggt gtgccacgtg
    17461 atgtgcctgg gggtgcggca gcacgcggcc ggcatcagta atggctgaat caagatgcgg
    17521 ctacggatga ggcttcgggg ggtatttgac gacggaggaa tcttgacggt ttcatcccga
    17581 aattgttatg gacaacgtga ggtagcgaca tcgaaacggg gagaaggcca tgcggcgaca
    17641 aacgaagtgg gttttgggtg ttgttctttt cactttcatt gtgccgctgc tcctgtgtgc
    17701 tgcgccaccg ggaatgctta attcgggcaa cgccgaaatt tccggcaagg tgactgatct
    17761 caaaggcacc cccctcaagg atatcacggt cgaagtccgg cagcagagcg gccaggcgtg
    17821 gcccgtagta aagacaaaca acacggatgc ggccggtctt tacaccgtcg ccggccttgg
    17881 cgacggcagc tatattgttg ctttcgatga ccccgccgga gtctacgccc gcgaaatcta
    17941 tgaaaacacc ttcttggtgt acaacgccca cacggttact gtgaccgcgg caagcccggc
    18001 caccgacatc aatgcctcgt tggcgctggc gggaaaaatc tccgggacct tcaccaattc
    18061 ggcgggctct cccttgcaat tcgcttatct cgtgagtttc cgcaaaaacg gggtcggctg
    18121 gcagccaatg gagagcactc tttctgatgc cgccggcaac tatgccctgg ccggcctgcc
    18181 tgccggaatc tatcgaattt tttacacgta cgaaggccag gagggttatt acgacaacgc
    18241 ccccgatctc ctcaccgcca ctgatatttc catcgctgcc gggcaagcgg tctccaatat
    18301 caacttcacc gtgggtgaac gctccaatgt tggcgccatc tccggcaacg tcaccgagga
    18361 agacggcgtc acccccctcg accatattta cgtcaccatc ttccgttggg cggatatttg
    18421 gtggcagcgg tatacggaag gagtcaccga tgccgagggc aagtacctta ttgaaaatct
    18481 tccacccggg aaataccgcg tctatttcga ggcctcccac tatggcgagg gcaagtatta
    18541 cgataacgcc ccggacattt tcagcgccac cgatatcacc gtcagtgccg gcgaaaccac
    18601 cacaaacatc aatgctgcca tgtcccccgg tgctgccacc gtacggggcg aatccggcgc
    18661 catcgccgga accgttacgc agcaggacgg caccaccccc atcgcgcaag tccatgtgac
    18721 ggcatttcgc tgggtggatg tgtggtggca gcgttatgcc gagggcatca ccgacaccga
    18781 aggcaactac tccatcacca acctcccccc cggcacatac cgcattcaat ttgaagcatg
    18841 gcacggcggt ttcggtgaat tctatgacaa cgtccccgac attttccacg ccaccgatat
    18901 taccgtcgag gaaggacaaa ccaccgccaa cataaacgcc gcactggcgg ggcagggctg
    18961 ccccgattac gaaacctggt ccgcatccat tgactggcag ggggcaccca acgggccggc
    19021 tgatgatgcc gatggcgacg gcttctccaa cgatctggaa cgcatagccg gaaccaaccc
    19081 cgttgcggcg aacgacttct tcaccgtaag ttccgctcaa ctcactcccg ccggcaacgg
    19141 tttgatcctc ggcaccgccc tgtttggccg cgcgtatacc gcccgctact ctaccgacat
    19201 cagactgccg tggcgcgaat ggacgcccat cgagttgtcc ttcagcgacg gcctcatcat
    19261 tgcccccgtc atccccaacc agcccaacgt catttaccgc ctcatcgttg acttggaata
    19321 gggtgggaag aacatgtgtt tgtttttctg aattacgcgc gcctcgaaaa tcctgttgtc
    19381 attatcctgc ttggattgta gggccgaaac ggtcgcggac cttccggcgc tcccggcggg
    19441 aaggtttcgt gcctcaacca ttcccgctca acaaaagcat ccgggctgga ttgtggaaat
    19501 cagtaacagc cgccggcctc agcgagaccg gctacagcag tcggaatggg ttggactgta
    19561 ggcgggggcg gtgaccccgc tgccatgcga ttttttaatc gaaaaaaaac cgatctcaac
    19621 ggattgtcgg gctgcaacgg ctgcaggtct tccatatgga aacatttctc cagcggcgcg
    19681 tttgcctgtg ggcgatgaag cccagctcgg cgtttatagc tgatgttcgg gtttgtcgag
    19741 cagtgggtga tccggcgcca ggcgcattat catgtcgcga aacggtcgga cggcatcgcg
    19801 gtcaccctgc ttcacgtggt aggcgagctg ggcaaagagc caacttagcc aggcatcggg
    19861 gtgggtctct tccggcggtt cgtaagtgcg gagccagtcg cgggcttcgt ccatgcgtcc
    19921 ttgacgagtc ataatgcgca agagggctga agcggcgaag agatacgtgg gatgttcgcg
    19981 gagaatgtca cggaggatag tttcggcttc ggcgaatcgg cccatcgcga gcagcgtggt
    20041 agcataattg aagcgggcgg gaaaaacttc cggggcgttg gtgaacacct ttttgaaacc
    20101 ttcggcggca atgggggcat tggctgggtt ctgctgcatc agctccaccg agtccttaaa
    20161 gagcttggcc cacttggccg ggggatcggg acaaaatcgg taatcgggat tcagcgtgtg
    20221 gcgtcgagtg atgacgctcc gctgtttgcc tttaaaccac aaatcttgtg gcttgccact
    20281 gccaggggca tcgcccttga ggagcatcgc ggcgcgcgtc cgccgggcgt cgggaccggt
    20341 gacgcttcgg gcgatgatct caagcagctc acgggcggcg ggatgctgtg attcgcccaa
    20401 aagagtcaat ccgtgatcgg ccatatcctc gccggcattg atgaggcttt cagccaaatc
    20461 caccgcggcg atggagtgag tgaatacttc gtctcgcagg gcctgttggc tcatgccgta
    20521 caccggcaaa ggaatgagtt catcgggagt gaaatagggc cagttgcccc ggatagtgtt
    20581 ggggacggca tggtcgcgca gcatgcggcg gtagcgcttg gccaaggcat tggcgggttc
    20641 gcccgcacgc tgggcaattt tcaggaactt ggccgctcgt tcatattcac ccagatttgc
    20701 cgccgccacg ccgccgtaaa aagccagtga ttcattgcgt ccgaaatcgc tggtttccag
    20761 cagggtcaaa atctcctgat gccagccata aagagccaaa gacttgcacg tgcgcaggac
    20821 gctgtcaaag ctgaccgagt gcatctcgag cgcccggcgc aatgcgtcct ccgcgagttg
    20881 cttttgttct ttggggtggg gcaaatagcg gcagtacagc gcgaggctgg cctggccgaa
    20941 cggattcgaa cgcggcacgg ccaatgcctt ctgctgggtc tcaatggcct tttcaatttc
    21001 gtatttctga aagtaacaca gcgccaggtt attgagcgcc ggcgtgtaat ccgcgcaggc
    21061 cgcgtaggcc tgttgaaaga ttttgatggc ttcgtccaac tcaagccgat gcagcagctc
    21121 gttgcccttg acgttgagat ccatggcggt gcgcaggcct gcatcggctg ccgacggtgg
    21181 caggggggcg gtgccgactg gctcgccggc gacgtggaat gtcgcggaaa acgaagcggt
    21241 atccggtttt ttgcggtagc aacaaaactt gtattttttg ccgctgccgc aggggcaagg
    21301 actataaggc tcgattctca tgtcgggttg gggcgaaggt ctgattcaat tttgaatcag
    21361 gctagtacat gcccacgcaa ttgaaaaatg aaatctgcgg gagatggtgc ttatgagagg
    21421 aaaaaccctc tcccgcggcg ggaggggagg gagggtagga tggggcgcgt aatggctggc
    21481 ctgtaactga ggagaaaccg atgaacaaaa tggcgttggg tgtttgggcg gtttggatcg
    21541 caacggccgg gtgggcggca gagacaccgt cgttctatgc ggggatttcg ggtggcttgg
    21601 cgatgcccat gaatatgctg gaggaatgga gctggccgga acgcggtcaa caggccgatc
    21661 tgaaggaaga gatacacaac ggcttcgtga tcggcgcgaa agcgggctgg ataccggcgg
    21721 tgctgggggg gcggttcgcg gcggagttgg aatacagcta ccggcgggcc gagttcgaca
    21781 ggatcatgtc gccgggcttc gcggcggggc cgtacaccat cgcgggcttc tattccccgg
    21841 cgacggattc acatattgag tttcagtcgc tattcgtgaa ttttctgatt cgctatccgg
    21901 acggcagggt gcatccctac atcggcttcg ggccgggtgt cacgcgttgc acggtgtcgt
    21961 tcaacgaagc cgagttgaca accgagggtt ttggattcgc ggagtccggg gacgacacaa
    22021 ccttttgctg gcaggcattg gccggggtgg acttcgacat ctccgcgcgc gtgatggtgg
    22081 gcgcgggcta ccgctacttt gccgcacaac cgtcgttcac ttgggcgaac ggcacacaga
    22141 gcgattacga cccggtctat cacgacgtgg cggtggacct aaaattccgg ttttaaaaag
    22201 tggcaggaga gaacaaagat gcaaaagaaa cgcatgatgg gctgggtggc tagaatcgcg
    22261 ctggtggggc tgctggcggc cggggtggcg agcgggcagc cggagatcac cgcgttcaac
    22321 ggtaacgggc agattagttg ggttgacccg ggcggcacgg gcacgcatta cgcggtgcag
    22381 tggtccacgt cgggcttgag caattggatg tcgtggcagg atgcggaggc cactttggcg
    22441 gggctggggc cgacgggctc ggcggcggtg ccgatgttct accgcgtggt ggcgcaagac
    22501 cccacctaca agcccaccga gagttatacc tatttccaaa aattgccgtt gtttgatcca
    22561 ttgaccccgc tggccaccaa cgaaatgcgg atcacgttca tgggctcgat gattcccttg
    22621 cccgtgcggt tggcgcaggc agaaatgagt atttttgtgg aggtggggtg gagcaacgat
    22681 cccaacgaca aggtgtatgg cgggcgggca cgcgaccaat tcatcttcga cgccggcgcg
    22741 ggcatttccg ccaactacgc cgcggccaaa gtcggattcc ggcgaatgga caaggtgttt
    22801 atcaaccatc tgcacggcga ccacatgaac gacttgacgc acatttattg tttcggccca
    22861 gccagcgatc gcaagtcacc gctgtatgtg tggggctcga agtcctccgg cgtggcgtgt
    22921 ccctccaacg attgggatat ctataacgac gatggcgtgg tgtacgacga cggtacgagc
    22981 aacttctgcg cgagcctacg cagcgcgtgg cgctggcact cggagagttt cagtttccag
    23041 ggtaccagct acacgaacta tgtgccgccc accaaggaaa gctgggggtt gccacacgat
    23101 cccgtgcccg tgggcaatga cgacccacgc gatgcctacg ccgtggtgcc gattgaactc
    23161 gattggtcgg tggtgggtgg cgtggcctat agcaacgcgg acacgggcgc aactatcacg
    23221 catttcccgg tgatccattg tcgccgcggt tccatgggct acaagctgga ttggctcacg
    23281 ccggagggcg cgaccttgag catgatctac accagcgata ccaagcccga aaccaactgt
    23341 atcgagcaag ccaagaacgg cggaaacggg gtggatgtgt tcatccacga aatggtcgtg
    23401 ccgcccgagg tctgggccta caaaaacatg ggcctcgacg cgccaccgca acccggcgat
    23461 cccaactacg ccgagtttca agccacggtg caagggctga cgtttgtgca aaacagctcg
    23521 cacacgccgc agggcgcatt cgggtacatt ttgagccaga ttaatccgcg gcccaagctg
    23581 accgtggcca cccatttccc cgtggcggat gacacagtgg ccagcgcgtt tgaaagcgtg
    23641 cgggcgcatt gcccggatgt cgagatgggg cgcgatatcg tgtggtcgtt cgacctcatg
    23701 gtgctgcggg tgttccccga ccacattgaa caggcacgcg cggatgtgtc gcggtacagc
    23761 ttcaacccac cggtccagtt gccgaccggc atgcgcccgc cgaagtacaa cgatggcatg
    23821 ggcaagggcg atcccttcta ccagattgac ctcagctcgg aaatcccgcc ggtctccggc
    23881 ggcgtgacca actaccgcgt ggatggatac tgagtttagt gtgtcggaaa aatgccgggc
    23941 gtaaaaacgc gaatacattg cgcccgcgcg ggttgggttg gccgcgcttg gattcggcct
    24001 ttgacatggc tcaccccatc ggtctagact tttcagattg tgaatgtggg aaaggacctc
    24061 ctatgcgtta ctccatcctc ttaagcgtga ttgtcttggc ggtggggtgc cgcgaaaaaa
    24121 tgcccgcccc cccgccaggc ccggttcccg tggtggccgc cgatgtcatc gtgcgcgacc
    24181 agccgatcta ttacgaggcg atggggcaaa ccctgggctc gcaggatgtg gaaattcgcg
    24241 cgcgggtgga gggtattttg gagtccgtcc acttccgcga aggcgccatg gtggaaaaag
    24301 gcgcactgct ctacaccatt gatcccagca cggtggaagc caacctggcg caggcgcgcg
    24361 gcgcgctggc gcaagcagaa gccgccgatg acaaggcccg tcgcgatgtc cgccgactga
    24421 cgccgctctg ggagaaaaat gcgatctccc gccaaatgct ggatgatgcc cttgccgcgc
    24481 aacattcggc atcgggcgcg gtggtgaccg cgcgcggcgc ggtggataac atggagattc
    24541 tgcttggcta cacgaaaatc catgcgccga ttgccgggct catcggcaaa accgaagtca
    24601 aacccggcaa cctggttggc cgcggccaga atacgctgct gaccacgctt tcggtgttgg
    24661 accccatcgc cgtgcggttc agcatcagcg aaaaagaatt tctgacgttg cagcacgtga
    24721 ttccggaaga ccagcaggcc ttggccagcg cggcgatttt tgagctggtg ctggccgatg
    24781 gctcctccct gcccgataaa gggaaagtgg tgtttgtgga ccggagcgtg gacccggcca
    24841 ccggcaccat gctgctggaa gtggcatttc caaatccagg gctgttggtg cggccggggc
    24901 aattcgggag ggtgcgcgcg ccgatccgga cggttgcggg tgcaatcctg gtaccgcaac
    24961 gcgccgtgca ggaattgcag gggacccact cggtttttgt ggtcggcgag gatggccgcg
    25021 cggagttccg cccggtgcgg gtggggccgc gggtggataa cctttatgtg attgaatccg
    25081 gcctgcacgc gggcgaaaaa gtggtggtgg agggcgggca gaagctgcaa aatggcacgg
    25141 cgattgccct tcagccggcc gccccggcca gcgagtaagc catggcgcag ttcttcatcc
    25201 gccggccaat tctggccatc gtgctggcga ttctgatggt gctgatcggg gtgcagaccc
    25261 tgctcacgct gcccatcgag cagtatccgc aactgtcgcc gccgaacatc caggtcaccg
    25321 cggtatatcc cggcgcgggc gccgaggcgg tggagcagtc cgtggccacg ccgattgagc
    25381 agcaggtcaa cggcgtggag cgcatgctgt acatgaagtc gctcaactcc agcgacggcc
    25441 ggatgcaatt gagtgtcacg tttgatgtcg gcagcgacat ggacacggcc aacatgctca
    25501 cccagaaccg agtggcgcag gcggaatcgc gattgccggc ggaggccgtg gcgcaggggc
    25561 tgacggtcaa gaagatcaat cccagtatcc tgatggtggt gagtctgtat tcgccgaatg
    25621 gaacctacga cagcctcttc ctgaacaact acgccgtgct caatgtccgc gacgccctgt
    25681 tgcgggtgcc gggcctctcc caggtggaca tggccggcgg cgcggaatac ggcatgcgca
    25741 tctggctgca accggacaag ctggccaagc tgggcctgac gccggccgat gtgatgagcg
    25801 ccatccgcga acaaaacgcg caggcgccgg cggggcaggt gggggccgcg cccgcgcccg
    25861 ccgggcagga gttcaccctg accgtgcgcg cgccgggccg cctgaccacg ccggaggaat
    25921 tcggcgagat tttggtgcgc tccaccgagg atggccggca ggtgcggctc aaggatttgg
    25981 cgcgcatcga gctgggcgcg gaaaattaca aatcgtttgg tcgctacaac ggccgcgagg
    26041 cgggcctgct gatgctctat ctgctgccgg gcgcgaacca gatcgagtgc gccaaagaag
    26101 tgtaccgcac gctggacgaa ttgaagcgct atttccccga tgatatggat tacgccatca
    26161 cctacgactc cacgccggcg gtctcggcct ccattgagga aatcgtccac accctgtttg
    26221 aggccattgt gctggtggtg ctcgtggtgt tcatcttctt gcaaaacttc cgcgccacgc
    26281 tgatcccgtt ggcggccatt ccggtttcgc tggtcggcac gttcattttc ttcccgctga
    26341 tgggcttcac gatcaacacg ctgaccatgt tcgggctggt gctggccatt ggcatcgtgg
    26401 tggacgatgc cattgtggtg gtggaggcgg tgatgtcgca catcgagcgc ggcaaatccc
    26461 cgcgcgaagc caccgcgcag gccatgcgcg aggtgaccgg gccggtcatc ggcacctcgg
    26521 cgatcatggt tgccgtcttt gtgccggtgg ccttcattgg cgggctgacg gggcgcatgt
    26581 atgcgccgtt tgcgctcacc atcgcggtgt cggtgatttt gtccacgttc aacgcgcttt
    26641 ccctgagccc ggcgctggcg agcctgatgc tgcggcccgc acatattgag caatcgccgc
    26701 tgcggtggtt ttaccgcggt ttcaaccggg tgttcacccg caccaccggc ggctatctgc
    26761 ggtgggccac cgcgctgggg cggcgcgcgg cgtggagcct gctgatcatc ggcggcgtgg
    26821 cgttgctggg cggggggctc tcgtcagtgg tgccggcggg gtttgtgccc attgaggacc
    26881 aaggcatttt gctggccaac ttgcagttgc cgaacgcggc ctcgctggag cgcaccagcg
    26941 aagccgcccg gcaagtggag gaagtgctgc gcaccacgcc gggcgtcgag tcctataacg
    27001 tcatcggcgg catgtcgctt ttgagcggca cctacacgcc caacaccggg acgtttttca
    27061 tccggctgaa accgtgggcc gagcgcacca cgccggagac cagcttaatc gggctggtgt
    27121 tggggctgcg ccaacggctg gcggcgattc ccgaagcggt ggcgttcccg tttgtgccgc
    27181 cctcgctgcc gggattcggt gcggccggcg gcttcgacat gctgctgcag gaccgcagcg
    27241 gcaacctgtc ggtggaggaa ctgcacgagc gcgcccaggc cttcctggcg gccgcgcggc
    27301 aacggcccga attgaccggg ctcttttcgg ctttcgaccc caccgtgccg caggtggagc
    27361 tgacggtgga ccgcgaaaaa gcgcgctcgt tgggcgtgcc gatttcggat gtgttcgcga
    27421 cgttgcaggc ctcgctgggc ggggcctatg tgaacgactt caaccgcttt ggccggctct
    27481 accgcgtctt tgtgcaggcc gactcggact tccgccggcg gccggaggac atcggccaat
    27541 tttatgtgcg cagccggacc accaacgcca tgattccgct ctccacgctg gtcaaggtga
    27601 cgcctgccgc cggcgcggag ctgaccattc gctacaacct gatgcgctcg gtgcaaatca
    27661 ccgggcaggc ggccgaagga tatagctcgg gccaagccat ggccgcgctg gaagccgtgg
    27721 cccgcgacgt gttgccgcgc gaaatgggca ttgctttcac cggactttcg tatcaggaga
    27781 aaaacgcccc cagctcggtg cccaccatga ttctggcggt ggtgtttgtc ttcctgctgc
    27841 tggcggcgct ctatgaaagc tgggcgctgc cgtggagcgt catgcttatc acgccgaccg
    27901 ttctgctggg cgcgtttctc ccggtgtggc tgctgggctt cgacaacaac atctatgtgc
    27961 aaatcggatt gattatgctg atcgggctgg ctgcgaaaaa cgccattctg attgtggagt
    28021 tcgccaagat gcagcgagat caaggagaag cgccgctggc cgccgcgatg gcggccgcca
    28081 agctgcgctt ccgccccatt ttgatgacgg ccttttcctt catcttgggg gtgttgccgt
    28141 tggtgctggc cagcggctcc ggcgccaacg cccgcaagat gatgggcatt gcggtactgg
    28201 ttggcatgct gacggccacg ctgctcggcg tgttcctgac gcccgcattc ttcattgcca
    28261 tcgagcgcct gagtcatcgc cggcagggag gtgccgcatg atccgtcatc gccatgccgc
    28321 cttgattttg ctgcccctgc tgctggctgg ttgcgccacc ggtccggact accagcgccc
    28381 agacttggcg ttgccggaag catggcgcgg cggcccggcc accagcgagt cgctcgccga
    28441 tctggcctac gcggattttt accgcgaccc ggtgctggtt gctctgatca ccaccgcgct
    28501 gacgaacagc ccggacgtgc gcgccgccgc cgcgcgcatc gaacaagccg ccgcggcgtt
    28561 gcgcgcccag cgtgccgact actggccggc cgtcaacggc accgccaact acacccgcgc
    28621 gcgaaaagga aatctgccgc cggtgccggg cgtggagggc gatcagtttg atgtctttgg
    28681 cgtgttgtcc tacgaagtgg atgtctgggg ccgcatccgc cggctcaacg aagccgcgcg
    28741 cgcccaatat ctggcgacgg ccgaagcccg ccgcaccgtg gagatcgggc tggtggcggg
    28801 cgtggcctcg gcctacttca atttgcgcgc gctggacagt cagttggcca tcgcccgcgc
    28861 aacactggcc tcgcgccgtg acatgctgga gctgacgcaa atcaaattcg acgacggcaa
    28921 cgggatcgtt tcggaattgg atgtggcgca ggcgcagacg caagtcgccg ccgcgcaatc
    28981 cgccatggcc aacttgcaac gggcgattgc ggcgacagaa aacgcgctca actggttgct
    29041 gggtggttat ccgggcgaca tcccgcgcgg attggacatc gtgcagcagt ggcagccgga
    29101 agacgtgccg gcaggcttgc ccgccgattt gctcctgcgc cgccccgacg tgtgcgcggc
    29161 ggaacaaaag cttattgctg ccaatgccaa cattggcgtg gcgcgcgcgg cctacttccc
    29221 ggcgctgtcg cttaccggcg cgctcggatt gcaaagcgat gatttggccg atttgttcga
    29281 taccggcttg gccaaggcgt ggacgttcag ccccaatctg gcggcgccga ttttcaatgc
    29341 cggcaagatt cgcgccggcg tgcgcgttgc cgcggcgcaa cgccaggaag cgctggccaa
    29401 ctatgaaaag gccgtgcaaa cggcgttccg cgaggtggcg gacgcgctga cggggcaaga
    29461 gtatctccgg caacaactcg cggctgatga agaaaccctg cgggcggaaa cccgccggct
    29521 ggatttggcc tgggcgcgct acgaaggcgg cgtggccagc tatagtgacg tgctcgacgc
    29581 gcaacgctac cagttcagcg ccgagctgac cgccgtgcaa acccgcaacg agctgctgca
    29641 atccctcgtg cagttgtaca aggcattggg cggcggctgg ggatcgtcgg cagccagccc
    29701 gacacaagcg aaaggaaatc catgaaaatt tatattgctg gcgccgcggg cggggaggtc
    29761 acgggttccg ctttttatgt gcaaaccgac caagcctgcg tgctggtgga ctgcggccag
    29821 tttcaaggct cccgccaagc ggaggcgctc aaccgctggc gcccgcccgc gcgcatcaag
    29881 ctgaatgcgg tcttgattac tcacgcgcac ttggaccata cgggccgcct gccgctggtg
    29941 gcacaaaaag gccacaaggt gccggtctat gccacggaag ccaccttgga gctgactgcc
    30001 ctgatcctgc gcgattccgc gcatattctc gccgcggatg ccatgcacaa aagccgccgg
    30061 ctggcacggg cgggcaagcc gccggtcacg ccgctctata gcttggagga tgtggaaaac
    30121 gccctccgca tgatgcgccc cgtgccgtat caggagccgg tgcaggtcgc gcccggcatt
    30181 caggccatct ggacggaagc cggtcacatg ctggggtcgg cctccatcca gttgattgtc
    30241 gaggaaggcg ggcggcaaaa gcgcatcgtg ttctcgggcg acctcggccc gcgcagcgcg
    30301 ccgattttgc gctcctttga gccgttccac gaggccgaca tggtgttcct agagtccacc
    30361 tatggctcgc gcgaccaccg gccgtttgtc aacaccgtcg aggagtttgt gggcatcgtc
    30421 aatgaagcca ttgcggcggg cggcaaaatg ctggtgccca ccttcgccgt cgggcgtgcc
    30481 cagctcattg ccggtttgct gggctggatg ttccgcaacc aaaaggcgca gccgttcccc
    30541 atttatatgg atagcccgat ggccattcag gcgtcggcca tctatacccg gcatcatgag
    30601 ctgttcgacg atgccacctt gaagttcatt gagcagggta cgctgcgcga tgagttgcgc
    30661 accatgaaac tgtgcgtgac ggcggacgac tctcggcaga tcaatcaggc ggccgggcct
    30721 tgtctggtca tggccggcgc ggggatgtgc aatgccggcc ggattctgca ccacttcaaa
    30781 gccaatctct ggaaaccgga aacgcatgtg ctgattgtgg gttatcaggc cgccggcacg
    30841 ctgggccggc agttggtgga cggcgcggag tacgtcaaaa tctttggcga aaagattgcg
    30901 gtcaaagcga atgtgcacac catgggcggc ttcagcgcgc atgcggggca gaccgatttg
    30961 ctcgcgtggc tggaggccat cgcgccttcg cgcccgcgcg tggtgctggt gcatggcgaa
    31021 actgaatcgc gccaggggtt ggcggtccag attcaacgcc gcttcgactt gcccgcggaa
    31081 ttgccggcga tgaacgaggt gcttgaattg taatcagaag cggcgcgcaa cgggccggaa
    31141 aatatgaata aaaaaatctt ttggggactc accgccggtt tgacgctggt cgtcatggtc
    31201 gcggtcgccg aaccggtgcc gccaacgttt gagtttgtgc ccagcttgtg ggtggccggc
    31261 ttgtccggct ccggcacgct ccacgggcag gacgtgaaat atgaccgctc cacgggcgat
    31321 ttgtgggaac acctcgaagc cggcggctca ctggcgggca aggtggtgtg gaacgggtgg
    31381 gcattgggcg gccaactcga cggctaccag ttatccaccg acgccctgac cgtgaatggc
    31441 acgcccctcg ccggacaaat ggatacggac ctgatttttg gggagctggc cgcgggtcgt
    31501 gtatttccgg gttggaagga aggccagacg ttcacgctgg cgctgggctt ccgttatgca
    31561 cgcatcgcga atgacttgga tgcccacggc accggctcct ttcatcatcg gcaggatcgt
    31621 tgggacccga tgatcatcct gtggcccagc ttgccgctgc tggccagcaa agttcaaggg
    31681 ttgagcctca cgcctgcgtt ttccatcggc ggtggtggcg attccaaact ggtgtatgaa
    31741 ctgttcccgc aagtgcgcta ccaagtgact ccgcacctcg tggcgcgggc gggctatcgc
    31801 gccatcggct accgggccaa gcactccgcg gacgaactga aattcacgct ggcgggcttt
    31861 attgtcgggc tgggcgtgac cttctgaacg gcgcggccct ttttgcggtt tgacattccg
    31921 cgggcgggac cggcaccatg cacaagccat gagagatttt ccggttgaag ttgtggcctg
    31981 ttttgtcggg ctggtactga ttgcgttgtt cgcgcatatc ttgttccgca agttgcggtt
    32041 tccctgcaca gtcggcttgg tgtttgtggg catgctgcta ttcaagctgg gcggcgtgtt
    32101 tgagccgctg aacgtcctgc aacgcgtcca cctgacacct aattttatcc cgaaaaatgc
    32161 atttgggcat aggggttgcg tcatcacccg atgaacactg g
//
LOCUS       DAOPNP010000012        29959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_1988_length_29959_cov_8.158407, whole genome shotgun sequence.
ACCESSION   DAOPNP010000012 DAOPNP010000000
VERSION     DAOPNP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 29959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29959
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_29959_cov_8.158407"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6365)
                     /locus_tag="PKI64_02170"
     CDS             complement(<1..6365)
                     /locus_tag="PKI64_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD99845.1"
                     /translation="MKSGQQLGRKWWRRIIGVGALGLLLPGMAYGEAMLQYFNTSWRE
                     MTDKIPELAEAGYSSIWIPPPTKGSGGLSVGYDLWDPFDLGGRHQRSTTATRYGTEAE
                     LLNLIETAHRFGLRVYADNIMNHRAFDIPGYNETTPVDVYPGMCPEDFHLRRTEDGFY
                     RKWDNCRDWNDAWQVMHLGLSDLIDIAHETPNQNHGLNEGDWNPKPSYVRHPHNPEFY
                     DRMPNTNVPPAQWNVWDWSGNTNANIYIGFGTNNGLTTNIIAQYPDFFKEDVGAYLLR
                     AVRWEMAMTKLDGLRLDAVKHVPDYFYGEFDKNDSNLGYCGNIQWQFNMTRGFSDWDN
                     HRDSNFNTEIPRDDALIFGEHLGQPPGYGGYWSAGMRLVDNDLRNQLNDRLGSPWNGL
                     EGFDNPGWGGFSPNLGVMHAQSHDNDYAARRELQHAFYFLREGLGLLYTDGNFHAETL
                     GESGGAFPRWANTSFLGQWGDNRVPNLLYCHEQFARGYQRGVWSDGDFVAWERLDWRQ
                     AGSNAAQHVTMLVMLNDDYSNGQARDIRSSISYPHQAGGPYGSDAYLFNYSDYYYGFY
                     TYASALNTVVVPPGGYFIFGYKNPDPSGLWSSFGGKPITIYENGKIAGSVRVTRKDGP
                     DGDPAFNPYGLPDTNTTDFSYSIDIPRVTNASSVSFATRVDGSAGNVLMKLDGGMNLN
                     NTNHIGGDPRDNPPALANDVFLGYEQATFVKRIWPEKFAAADSNRCKLGSAGAETYVA
                     TIGQANWTINPSSGSNDWSDDQMVSWIYHNPQAMQDGPRVGNAQFWPPLTNAAGSTLY
                     IGIKTPKVGGNRVHLYYTTNGVAWPEGAGGAAGNRDTLVVECNWVGDGTNSTDWWEAQ
                     VPALPAGTVFRYKTGSAREQGYDGNGWEIVWPGSATDITRKADMMGVWQVTNLNLGTI
                     TNRPHNDYGETRTGLKEGFHLISARAFLNRDDGSAVYNTFKQTFYLDAQRPQGYVQWP
                     AANGDTLGGTEYGVVVRTDPTVREVWYRIEDSDASNDDSATSKSNGNGNVYAAWQKAA
                     RVTPSDMNRDYPQAWRFTYANLPTGGTATIRVRLREWSSAEKTAWTNAGLTEAQGHYT
                     ELIRTVNTSGDAYRLYFDWPGQDGDYVEAGWEIRVKYSWNFAENLDDAEALALFTIKL
                     NSAANGGDPAEGVILSPDDINLQHQWDYPNENTIKFTMPNVYNGHPDWLHSFEIVGTR
                     AGKPTLRATRQVRTMGELLPSIIITEPPEFGSDGKPHVIILKDVPPAEIATNPALRTT
                     RIQLATDTNAVATGIYFTSPTGYTGQVALVGSSNIGSTLFWDYAWSNVTAGTYRFTAW
                     VRDPDNKTNSASRTARLQLRQVVDMTSTNDLDHDDDGLEDAWENNVVPLPNGFPDTDP
                     RYKPNPEFWTNGDIHQHDAFGRSLPESPDSDGDGLPDGLELGWRTPSAHTDTNADTNA
                     DGWPNFRADLDPPFYNTLDNFGKVPGVDSQSAGGDRSKRLWGSTTDPNNPDSDYDGIP
                     DGVEDANRNGWVDGDGDSIAPGQDPSTRVNWPNGRMDSWETWLETDPNNPDTDGDGLS
                     DGYGEDKNFNGRIDGDSNSNRVWDAGEIWLETDPLNPDTDGDGLPDGWEVRYGLDPLD
                     CGIVGHTNMGTGLVIGSTEHGANGDPDGDGFTNIQEYLNGTNPRHYDDPGSPPPAGAI
                     TIGPGAAIGSVNGKTNYVEFTDWTLDDLIALDPYNTGGSQAVDIYRRWDGFDSSRDMV
                     AFYMRDGGAADDKLYFRVDFHDLQAHAEESALNIYVVLDFNSPGAGEAALPDEVDTRT
                     DMKWEAVVAVYDSQNGNLYVDTDPAHNTVGAWDSLSANGVQIAANGFQGAYFNSELDA
                     CEFAIRRSALTDIGWNGDASRLNFQVFTTKDGTCNSCNGGKPGAGDIGGRSDITDSIR
                     NDWICSDYWRDQDWIAQNGVLTQWIGRNADNDMGKSAKIALLAHGNQAIQPANIIHDI
                     VNNGAGAGYQRPIKSHNIFKKPLNLHITPTLASALQWAEVKDGGPEWRSGPGLNDMIR
                     QAVANSNVVLMASTYSDHALPYFTPAFNQDNVNLASATLNRIYGANINSNSVFWAPER
                     LLDADVFAKITNMGFRYTLVDQNTHVFNWFGRQVALGDDGYRINRIN"
     gene            complement(6524..7792)
                     /gene="umuC"
                     /locus_tag="PKI64_02175"
     CDS             complement(6524..7792)
                     /gene="umuC"
                     /locus_tag="PKI64_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HOD99846.1"
                     /translation="MFALVDCNNFYASCERVFQPALEGRPIVVLSNNDGCVVARSAEA
                     KALGIPMGKPWFKVAPAVRGAGVVVFSSNYALYGEFSRRVMQTLTQFSPRLEVYSIDE
                     CFLDVGGHRGDLTALGQAIARTVRRWTGIPVSVGIAPTKTLAKVANRLAKKGQAGQGP
                     VCHWGEVAAPREALAALAVEDIWGIAARWGGRLRQAGVATALALAEADPSWLRRLGGV
                     VLERIGRELAGCSCLPLELVPPAKKQIMVSRSFGERLSSLTDLQGAVAAFAARAGVKL
                     RRERLRAQAVTVFVQTSPFADAEPWYANGVTLVLERPTQDSGRLVQCAQEGLTRIFRP
                     GLRYQKAGVLLMDLTPAGAEQGLLFASQTPDSPATMRLMTCVDTLNREPARRVVCHAR
                     ELVSTRWQMRQQRKSVNRPTDWAHLPVVHA"
     gene            complement(7801..8181)
                     /gene="umuD"
                     /locus_tag="PKI64_02180"
     CDS             complement(7801..8181)
                     /gene="umuD"
                     /locus_tag="PKI64_02180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOD99847.1"
                     /translation="MLRLFSSRIAAGFPSPADDHLEGPLDLNAYLAPHPAATFVVRVT
                     GDSMVRAGIRDGDLLVVDRAREAASGAIVVAVVDGELTVKRLRVGREGIRLEAENPAY
                     PPIVVHEHTELVIWGVVAHAIRSY"
     gene            complement(8321..9085)
                     /locus_tag="PKI64_02185"
     CDS             complement(8321..9085)
                     /locus_tag="PKI64_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOD99848.1"
                     /translation="MAELFDKHGGFRRLHSFTLATIVQLETLRFCRRFLTADPRERET
                     KFYDPKGRQYDQMVQAARGGRQNIIEGSERAATSKDTEMKLTDVARASLSELRGDYEV
                     FILDRGELPWSVHSPEAKAINAVSLDAPAFQDDMVHESAKHAREQRKKYARWLDADDP
                     VVVANAMLMMIGRALNMLKSLIESQGKAFEKTGGFSERLSTKRREIRDQQKDAAAPAC
                     PQCGQPMRRRQSAKGDFWGCSAYPDCKGTRPGSIAP"
     gene            9265..10788
                     /locus_tag="PKI64_02190"
     CDS             9265..10788
                     /locus_tag="PKI64_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD99849.1"
                     /translation="MKTKTSWLSSAVIYQVNLRSLAAREPRNAVEALTEKPQPLSPLA
                     YLTKSLPRLRRLGFNVVYLLPPYPIGLLGRKGIGSPYASRDFRAVEPEYGTMEDIQAF
                     IRRAHELKLKVIFDITPNHTARDHVWMTAHPEYYVKAPDGSAFFDADWSDTAKLDYTN
                     HDLRAAMIEVYDFWLSCLGPDEHGAPDGVDGFRLDMAHFINDKSFWDQALPALRARHP
                     SRDLLFMAECYGFQNNLDLFARGMDAAYDDDFYKLCQYAYAVDEAGNSRVRLAADAQH
                     NHDFHPILNQWQDGGLAAAAAKVLRQYEEAAARFPCRHYTARYTDNHDEGRGLYRFGP
                     GAFRAMNILAFLAPRTLPFLLTGQESGALNRPSIHARCQPCDKGRRILTADGREVRQE
                     GVELEGNLFDRGYAARQQWQQFFKELIALRKKHRPLREGSCDILDLGEDAGPRDRTVL
                     AFDRKLGQQLLRCVINLGDQPRHLARANALLNGTPLYGTLPAAGVLPPFSVVVVSIA"
     gene            complement(10859..11239)
                     /locus_tag="PKI64_02195"
     CDS             complement(10859..11239)
                     /locus_tag="PKI64_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD99850.1"
                     /translation="MTTATEKRQDAPVPANEPVYIPRVDIREDAERFTVLADMPGADE
                     KTAEATVHNRVLTIEGWCQTGGCPTPGEELGCEFRAGRYRRDFTLPETVDAERIQARI
                     KNGTLTVTLPKREEVKARKIQVTT"
     gene            complement(11253..11768)
                     /locus_tag="PKI64_02200"
     CDS             complement(11253..11768)
                     /locus_tag="PKI64_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD99851.1"
                     /translation="MGSALYKVDKQLNRTKKGVLAMLELMIGPRSPWTIFDELESLQD
                     NMNRLLAGQEPRVERRAGAAYPPLNVYSTADGLTLVAEMPGVDPQDVEISVDGDELAL
                     RGKVNSAAPAAGETVLRRERPAGEFQRTLQLPFRAHAAGVKATFKNGLLNIAVPRSPE
                     EKPRKIAITAE"
     assembly_gap    11922..11931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11939..12406)
                     /locus_tag="PKI64_02205"
     CDS             complement(11939..12406)
                     /locus_tag="PKI64_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD99852.1"
                     /translation="MVDKLWQQQYRKMLFCTDFSANADRAFVFAVRAAQRNPGCELHL
                     LHVIPEPPAQFWKGYIYEVDEDVDARAKREIDAKLAEAYQPHVPPEVKLIIAMRIGEA
                     RQKILEYAQEQGIDLIILGRQGAGAVGKILFGNVTERVVRKAECPVLVIPPAK"
     gene            complement(12426..13727)
                     /locus_tag="PKI64_02210"
     CDS             complement(12426..13727)
                     /locus_tag="PKI64_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOD99853.1"
                     /translation="MTDLQVGLCGIALLFLLLATSMPVAFAMTLVGVVGYAVMVNGNA
                     ALYILVTDYYDTFSNYGLTVIPLFVLMGQVAFHTGISQRLFAAAQSWFGWLRGGLAMA
                     TVGACTAFGAICGSGPATAATMAAVALPEMKRYKYHAGFAAGTVAAGGSIGMMIPPSI
                     VFIVYGVMTEQSIGKLFIAGIVPGLLVAALFMLTIMLLCRWRPALAPPATAELWRARF
                     RALSGVVEILALFALVMGGMFAGWFTPTEAAAIGAAGSLVIAALARKLTWRMLATALN
                     ETLRTSCMVLVIVAGATVFGHFLQITNVPTSLAQWMANLPLPGWGTMLLIIGFYLVAG
                     AFLDALALVMLTIPIFYPVALKLGFDPIWFGVVIVLVTQMGVISPPVGVNVYVVSGMD
                     RAVPLASVFKGALPFLLALIVGALILLVFPQLVTVPVTWVR"
     gene            complement(13731..14237)
                     /locus_tag="PKI64_02215"
     CDS             complement(13731..14237)
                     /locus_tag="PKI64_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOD99854.1"
                     /translation="MSPLSGASARTGFLRRVIHGMAVVGGLAVLGIILVTVADVIGRR
                     CGLPVRGAYDLVRVLGAIAMVCALPLTKAVKGHIAIEYFFLKLRPRGRAVTDTLMRLV
                     LLLLFSLLAWQFVLQGNAFFESGEVTTTLHMPMFWVPWLAAAACVVTVGVTLWHLLHP
                     GRSLLRPR"
     gene            complement(14240..15289)
                     /locus_tag="PKI64_02220"
     CDS             complement(14240..15289)
                     /locus_tag="PKI64_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD99855.1"
                     /translation="MKKYLGLAGVGFVALGLALSGCGKRDAAGGDAAADKTYKLTYSV
                     FFPPMHIQAQTATEWAREVEKRSGGRLQITVYPGGTLTKADQCWQGVLNGISDLGMSC
                     FAYTPGRFPLLEALDLPLGWPDGLTATRMATELAAQFAPAEIQGAKLLYVHAHGPGIL
                     ATKKPVRTLADLKGMKIRGTGLSAGIATALGATAVGMPQPETYDALQKGVVEGTFCPV
                     ETLKGWKQGEVIAYITDTKAIGYTTAMFVAMNQKSWDALPTDLQEIITTVSAEWVDKH
                     GQAWNDADEEGWAFVRDLGREVITLPPEEVAGWKAAMEPILADYVQRATAKGLPGAEM
                     LAAIQTRLAAAESQP"
     gene            15451..16686
                     /gene="trpB"
                     /locus_tag="PKI64_02225"
     CDS             15451..16686
                     /gene="trpB"
                     /locus_tag="PKI64_02225"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOD99856.1"
                     /translation="MNVADYSNYFNLHPDKEGRFGKYGGAYLPPELEKIMEEIRDAYD
                     TISRSARFIAELRSIRKHFQGRPTPVYHAERLSRKLGAAQIYLKREDLNHTGAHKLNH
                     CMGEGLLATYLGKKKLIAETGAGQHGVALATAAAYFGLECDIHMGEVDIVKQAPNVAR
                     MKLLGANVIPVTFGLRTLKEAVDSCFSAYLKDPVTQLYCIGSVVGPHPFPMMVRDFQR
                     VVGIEAREQFHDMTGDIPDIVTACVGGGSNAMGLFSGFLADPCEIHGVEPLGRGPKVG
                     DHAATMTYGKEGVLHGFNSYLLQDEKGEPLPVYSIASGLDYPSVGPEHSFLKDIGRVQ
                     YHTITDREAVEAFFMLSRVEGIIPALESCHAVAHAIRLSRTAPKHSRSILVNLSGRGD
                     KDMDYVIEHYGFGENFKLD"
     gene            16801..16947
                     /locus_tag="PKI64_02230"
     CDS             16801..16947
                     /locus_tag="PKI64_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99857.1"
                     /translation="MRDETFPPEKAGRSATVPAFNPTRIATLTKDMDYVIEHYGFGEN
                     FKLN"
     gene            complement(16970..17734)
                     /gene="nuoB"
                     /locus_tag="PKI64_02235"
     CDS             complement(16970..17734)
                     /gene="nuoB"
                     /locus_tag="PKI64_02235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOD99858.1"
                     /translation="MNRLILERWRQKHRTVAYPAQPPALPDRLRGAPELNPDKCRADC
                     TQCVAACPTGAITRVNGVLQLDMGRCLFCGDCQAACPTGAIRLTHDHRMAVRRREDLV
                     VTGRERPLAVALDAKIKSLFGRSLKLRQVSAGGCNACEADTNVLTTVVFDLSRFGIRF
                     VASPRHADGLLITGPATENMALALQKTYDALAEPRLVIAVGACAISGGPFAGHAEVRN
                     GATALLPVDLFIPGCPPHPITILDGLLRLLGRLEDI"
     gene            complement(17731..19239)
                     /locus_tag="PKI64_02240"
     CDS             complement(17731..19239)
                     /locus_tag="PKI64_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOD99859.1"
                     /translation="MSLLTRHLAPIANDRIPLLPMKVFRQEILENIAAGQRLSVLAGT
                     PATAGNVRLVAALADDRSGGLRLLAALAGESYPALTPDCPQAAGFEREIAEQWAVIPE
                     GHPWLKPLRFHRAYRPGRDAWGRGDAPLAPSVMEFFRVAGEGIHEVAVGPVHAGIIEP
                     GHFRFQCDGEEVLHLEISLGYQHRGIERALLDGPHPLTIHHMETLAGDTTIGHAVAYC
                     QAVEALAGCVVPARARALRAIALELERLANHTGDLGALAGDVGFLPTASYCGRLRGDY
                     LNMTALLCGSRFGRGLVRPGGVMFDVDQARLDTLLTRLATTFRDTAGAVNLLWDSASV
                     MARFENTGAVAKPLAEELGLVGPAARASGCARDVRRDFPTGAYQGMTITPALGEDGDV
                     LARAQVRWQEIQQSVALVRTLATTLPPGPCHEPCGALQPEATVVSLQEGWRGEIAHMA
                     TTDSAGHFARYKVVDPSFHNWLGLAMALRNQQISDFPLCNKSFNLSYCGHDL"
     gene            complement(19236..20717)
                     /locus_tag="PKI64_02245"
     CDS             complement(19236..20717)
                     /locus_tag="PKI64_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOD99860.1"
                     /translation="MIRLLVLLPLAAAVAAMLLRAPGQRHALLVATAALHAALSVLAF
                     IRPPACAADAWLGLDPAGLLFLGLTSFLFLAVAVYAVHSLRQTRGSQGRQVEVDTGTV
                     FVNAPEAVFIACLLVFLSTMTLVTVSRHLGVLWVAVEATTLATAPLIYFHRQPSSLEA
                     TWKYLLVCSVGIALALLGNFLVVIAAAAADGTGGTLLVPELLARSVGLQPAWLKAAFV
                     FLLVGYGTKMGLVPMHTWLPDAHSEAPSPVSALLSGALLNCAFLGIWRAHAICAAAGL
                     AEFSGALLTGFGLLSMGLAAALIVNQSDFKRMLAYSSVEHMGLLAFGTGVGGVAMYGA
                     LLHALNHSLVKAALFLVAGNLVALYQTKSIAKVSGVGRLLPVSGALWLAGLMAISGMP
                     PFGTFAGEFTMLRAAFAQGQGGWALAALVALGVAFVGMSRAFLNMVYGDAPAGLAPAR
                     EARRAYGPSLMLLALSLALGLWLPNGLHRLLAQASLWAGGGVP"
     gene            complement(20714..21364)
                     /locus_tag="PKI64_02250"
     CDS             complement(20714..21364)
                     /locus_tag="PKI64_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOD99861.1"
                     /translation="MNAIETLLIALVLTNLVLLGASRLHNAIRIVALQGIGLGVLPIL
                     AHHGGMLWAAVLLGAGSALIKGLLFPAMLRRALRETHGHREMEPFIGFNLSLLCGVGA
                     LALSFWLGARLPLPWPPVADLLVPAGLFTVWTGLFMIVARRKAITQVLGFIVLENGIF
                     TLGIVLVEAMPLLVELGVLLDIFVAVFVMGIIVFNINREFDHINTDQLAALREDAP"
     gene            complement(21369..22268)
                     /locus_tag="PKI64_02255"
     CDS             complement(21369..22268)
                     /locus_tag="PKI64_02255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOD99862.1"
                     /translation="MNAAAILHPLAALLLAPLLPGVINRVKARMAGREGPPLLQLYYD
                     LWKLLRKGAVYSRTTTWVFRAGPRLNFVCLLVALLILPLGRVPAAVSFAGDLVLLAYL
                     LGLARFATVAAALDTGSSFEGMGASREVQFALFTEPALLIGMAALAHASGDLSLTGIY
                     ADWPAALMPAMALLGSGFFILFLAENCRIPVDDPATHLELTMIHEVMVLDHGNVDYAF
                     ILWGSALKLWVVGALLVGLFPAAATGCPLLDLAVGFLWLVVLAILTGLVESVMARVRL
                     LRVPQLLVGAGVLSTLGLLLTFW"
     gene            complement(22265..24244)
                     /locus_tag="PKI64_02260"
     CDS             complement(22265..24244)
                     /locus_tag="PKI64_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOD99863.1"
                     /translation="MNHDATILLFVLLWAIGILPITGAAALWYGRWAERRAAAAGQLG
                     AVLACVVGFFVALAGLTRAVEGHIVLPWRTPAGELALGLDPLSSFFLLPLFLLCGLAA
                     IYAGGYFKTWGGRNPGRFWFFYNALVASMALVILARNGVLFLVAWEIMSLASFFLVVM
                     DDTASSREAGWIYLVATHLGAAFLIALFALLGRAEGSQDFATWRAGGAAGLLFTLALL
                     GFGAKVGFFPLHVWLPEAHPAAPSPVSAVMSGVLIKTGIYGLVRMLLILGEVPAWGAW
                     VLLGIGAVSGIPGILFALAQRDLKRLLAYSSGENMGIVALGLGLGLLGLHYQSPAVAA
                     LGFAGALLHVLNHALFKGLLFMGAGAVAQATGTRNMEQLGGLLKRMPLTGLAFFAGAA
                     AICALPPLNGFVSEFLIFVASFQALLATGGVALGGLVVVVSLALIGGLALACFTKAFG
                     VVFQGEPRTPAAAGATEAAASLTVPILLLAGLCLALGVGAVHVVRGLVPVVTQLAGTD
                     AALLAATRVPAVLSGLFLVLIAGALAMAWVRRRLLAARSVRETVTWDCGYAAPAPSMQ
                     YTGASFVQPLTHGAQAILQAQSRQQDPVGIFPAAVSRTAEVPDVARRVFFDPLFRWVG
                     AGLGWFRWVQEGRVHFYVLYIVVALLALLGWALWS"
     gene            complement(24361..25062)
                     /gene="trmD"
                     /locus_tag="PKI64_02265"
     CDS             complement(24361..25062)
                     /gene="trmD"
                     /locus_tag="PKI64_02265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOD99864.1"
                     /translation="MAAAPLHIDIITIFPRMLDGILGESILKRAQEAGLVTIRCVNLR
                     DFATGAHLTTDDRPYGGGPGMVMKPEPVFKAVDHLRRPESKVILMTPQGEPFKQATAQ
                     ALAQAAHLIFICGHYEGVDERIRTALADMEISIGDYVLTNGVLAAAVVVDAVVRLLPG
                     ALGGEGAADDESFTTGLLEYPQYTRPPVYRGLGIPEILTSGNHAEIAKWRREQAIART
                     RARRPDLPPPSADAP"
     gene            complement(25072..25302)
                     /locus_tag="PKI64_02270"
     CDS             complement(25072..25302)
                     /locus_tag="PKI64_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOD99865.1"
                     /translation="MKELIEYVVKALVDHPDELRLTEIEGERTIVFELRCHPEDVGKV
                     IGKNGKTVGAIRTLLSTVASRQNRRAMLEVVE"
     gene            complement(25366..25614)
                     /gene="rpsP"
                     /locus_tag="PKI64_02275"
     CDS             complement(25366..25614)
                     /gene="rpsP"
                     /locus_tag="PKI64_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOD99866.1"
                     /translation="MVKIRLRRMGANKKPFFRIVATDVRSANAGNFLEELGWYDPAKK
                     SNNYQLDLAKAEAWLAKGAQPSETVAALIKKSRKAAAQ"
     gene            complement(25705..27042)
                     /gene="ffh"
                     /locus_tag="PKI64_02280"
     CDS             complement(25705..27042)
                     /gene="ffh"
                     /locus_tag="PKI64_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOD99867.1"
                     /translation="MFDRLTSRLQTAFRNLRGVGQLSEKNVQDALREVRLALLEADVH
                     YATAKEFIERVKAKSLGATVLAGVAPGQQIIKAIHDELVALLGGEHRDFNLNAIPAEV
                     MLLGLHGSGKTTTSGKLARLWKQKGKKVLLVACDIRRPAAVDQLEVLAKQAGAGIVKP
                     QPGESVPDLGRRAFQFARQNYYDIVLYDTGGRFQIDEELVAELQALRAATEPQNVVLV
                     LDAAIGQESVNVAKTFHDALGLTGLILTKLDGDTRGGAALSVVAVTGCPILRVGTGER
                     PEDLEVFHPDRMASRILGMGDVVSLVEKVQETMDVAEAEKIQAKFKSKEGLDLNDFLA
                     QMRQIKKMGSLGKLMEMLPGAGAIAGDIRNRIENDSGDQMKKFEAIVLSMTPRERRHP
                     DLLNGSRRKRVAKGAGVHVSDVNTLLRNFRDARQMAKRMQRLQKRLPRGGLFR"
     gene            complement(27050..27529)
                     /locus_tag="PKI64_02285"
     CDS             complement(27050..27529)
                     /locus_tag="PKI64_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99868.1"
                     /translation="MNTPRLPPAQFKYRNVYIVLMAGVVIAGFIWQLKWMQQVRRTGA
                     ENIAPNSFMNQLGNWMLETRGGAAAGGANLAPGVETLECNNCLGTGSVLSETGERAIC
                     PICLGVGSRLIRRQDDLDRICPLCAGMGRTTLPDTDEVGTCPRCGGRGLVRPAEPAP"
     gene            complement(27526..28386)
                     /gene="prmC"
                     /locus_tag="PKI64_02290"
     CDS             complement(27526..28386)
                     /gene="prmC"
                     /locus_tag="PKI64_02290"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOD99869.1"
                     /translation="MTGPQPWSALLAAAERRLRAAAVEEADTKLRWVAAHLLGCGLAD
                     VFRHAAEIPTPEHAAAFAAAVERLATHEPVQYVIGETDFMGLRIRCDRRALIPRPETE
                     VLTDHAEDFLRELPAPRVVDVCTGSGCIACALAKRVPHARVWGTDISADALDLARENA
                     RALGAAVEFRQTDLLTGFADDSMDMVIANPPYVSTADCARLARTVRDFEPHLALDGGV
                     DGLRLISRLVSEAARVLTSGGKLLLEIAEDQAVVVPELLCAQTQLSLLSRKSDYAGNW
                     RVVIAARNPR"
     gene            complement(28383..29480)
                     /gene="prfA"
                     /locus_tag="PKI64_02295"
     CDS             complement(28383..29480)
                     /gene="prfA"
                     /locus_tag="PKI64_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOD99870.1"
                     /translation="MELDPLYFKQVATRLEELEQAMAAPGAASHPQTFKELVREHQHL
                     RELNAAAEEYRRLERLLADNAALLAEAAGDPELAEIAQAEKEELTAALSRQERKLMAL
                     MLPPDPDDSRNTIMEIRAGTGGEEAALFAGDLFRMYSKFADSQGWKTSVMDAAHSDLG
                     GFKEIIFSVEGTDVYKTLRYESGGHRVQRVPETEAQGRVHTSAATVAVLPAVEEINEV
                     EIKPEDLRIDLFCASGPGGQKVNKTESAIRITHLPTGLVVACQDERSQNRNRDKAMRV
                     LAARLLDLKRQQEHDKRTSERKTQIGSGDRSERIRTYNFPQNRLTDHRINLTLYSLDQ
                     IMEGKLEELIGALQARDVELRLAEQINGAKG"
     gene            complement(29565..29783)
                     /gene="rpmE"
                     /locus_tag="PKI64_02300"
     CDS             complement(29565..29783)
                     /gene="rpmE"
                     /locus_tag="PKI64_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOD99871.1"
                     /translation="MKKDIHPKYDEATISCACGYVLKTRSTVKSMNINTCANCHPYFT
                     GQAKFIDTEGRVDRFNKRYQRKPATAKK"
BASE COUNT         5716 a        10149 c         8817 g         5267 t
ORIGIN      
        1 ttattgatcc ggttgatgcg gtagccatca tcgcccagcg ccacctgccg gccaaaccag
       61 ttgaagacat gggtgttttg atccaccagc gtatagcgga agcccatgtt ggtgattttg
      121 gcgaacacat cggcatccag cagacgctcc ggcgcccaga acacggagtt gctgttgatg
      181 ttggcgccgt agattcggtt gagagtggca gaagccagat tcacgttatc ctgattgaac
      241 gcaggcgtga aatacggcag cgcgtgatcc gaataagtac tggccatcag caccacgttg
      301 ctgttggcca cggcctgccg gatcatgtca ttgagtcccg ggccggaacg ccactccgga
      361 ccgccgtcct tgacttcggc ccactgcaac gccgaggcca acgtcggcgt gatgtgcagg
      421 ttgagcggct tcttgaagat gttatggctc ttgatgggcc gctgataacc cgcgccggca
      481 ccattgttga cgatgtcatg gatgatgttg gccggctgga tggcctgatt gccatgagct
      541 agcagcgcaa tcttggcgga cttgcccata tcgttgtcgg cattccggcc gatccattgg
      601 gttaggacgc cattctgggc aatccaatcc tgatcacgcc aatagtcgga gcaaatccaa
      661 tcgttacgga tgctgtcggt gatgtccgat cgcccgccga tatcgcccgc gccgggcttt
      721 ccgccgttgc aggaattgca ggtgccatcc ttggtggtga acacttggaa gttgagtcgg
      781 ctggcatcgc cgttccagcc gatatccgtc aatgcactgc ggcggatagc aaactcacaa
      841 gcatcgagtt cggaattgaa ataggcccct tggaagccgt tggcggcaat ctgcacgccg
      901 ttggcgctca ggctgtccca cgcgcccacg gtgttgtggg cgggatcggt atccacgtag
      961 agattgccgt tttggctgtc atagacagcc acgacggcct cccacttcat atcggtgcgg
     1021 gtatccacct catccggcag cgcagcctcg ccggcgcccg ggctgttgaa gtccagcacc
     1081 acataaatat tcaacgcact ctcttcggcg tgggcctgca aatcatggaa gtccacgcgg
     1141 aaatacagct tgtcatcggc cgcgccacca tcgcgcatat aaaatgccac catgtcgcgc
     1201 gaactgtcga aaccgtccca gcggcgataa atatccaccg cctggctgcc gcccgtgttg
     1261 tacgggtcca acgcgatcag atcatccagc gtccaatcgg tgaattccac gtaattggtc
     1321 ttgccgttga cgctgccaat ggccgcgccg gggccaatcg tgatggcgcc cgccggtggc
     1381 ggcgagccgg gatcatcgta atgccgcgga ttggtgccgt tgagatattc ctggatattg
     1441 gtgaacccat cgccatccgg gtcgccattg gcgccgtgct ccgtgctgcc gatgacaagt
     1501 ccggtgccca tgttggtatg gcccacaatg ccgcaatcca gcggatcaag gccatagcgc
     1561 acttcccaac catcgggaag gccatcgccg tcggtgtccg gattcagcgg gtcggtttcc
     1621 agccagattt cgccggcatc ccagacgcgg ttggagttgc tgtcgccatc aatccgccca
     1681 ttaaagtttt tgtcttcgcc atagccatca ctcaagccat cgccgtcggt atcgggattg
     1741 ttgggatcgg tttccagcca agtttcccag gagtccatgc gaccgttggg ccaattgaca
     1801 cgcgtacttg ggtcttgtcc gggtgcgatg gagtcgccgt cgccatccac ccacccgtta
     1861 cggttggcat cttccacgcc gtcggggatg ccatcgtaat cgctgtcggg gttgttggga
     1921 tcggtggtgc tgccccagag acgttttgaa cggtcgccgc cggcactttg gctatccacg
     1981 ccgggcactt tgccaaagtt gtcgagcgta ttgtagaacg gcgggtcaag gtcggcgcgg
     2041 aagttcggcc agccgtcggc gttggtgtcg gcgttggtgt ccgtgtgcgc ggagggcgtg
     2101 cgccaaccca attccaatcc gtcgggcaga ccgtcgccat cgctgtcggg gctttcgggc
     2161 aggctccgcc caaaagcatc gtgctgatga atatcgccat tggtccagaa ttccggattg
     2221 ggcttatagc gcggatcggt atcggggaag ccgttgggca gcgggacaac attgttttcc
     2281 cacgcgtctt ccagcccgtc atcatcatga tcgagatcgt tggtgctggt catgtccacc
     2341 acttgcctga gttgcaggcg cgcggtgcgc gatgcgctat tggtcttgtt gtccgggtcc
     2401 ctgacccacg cggtgaaacg gtaagttcca gccgtcacgt tgctccacgc ataatcccag
     2461 aacaaggtgc tgccgatgtt cgaactgccc accagcgcca cctgccccgt gtagccggtc
     2521 ggcgacgtga aataaatacc ggtggccacc gcgttggtgt ccgtggccaa ctgaatgcgg
     2581 gtggtgcgca gcgccggatt ggtggctatc tctgccggcg gcacgtcctt gaggatgatc
     2641 acatgcggct tgccatcgct gccgaactcc ggcggctcgg taatgatgat ggacggcagc
     2701 aactcgccca tggtccgcac ttggcgcgtg gcgcgcagcg tcggcttgcc cgcgcgggtt
     2761 cccacaatct cgaagctgtg cagccagtcc gggtgcccgt tgtacacatt gggcatcgtg
     2821 aacttgatgg tgttctcgtt gggataatcc cactgatgct gcaggttgat gtcatcgggc
     2881 gagaggatca caccttcagc cggatcgccg ccattcgcag cactgttgag cttgatggtg
     2941 aagagcgcca gcgcttccgc gtcgtccaga ttttccgcaa agttccacga atacttcacg
     3001 cggatttccc agccggcctc gacataatcg ccgtcctggc ccggccaatc gaagtagagc
     3061 cggtaggcat cgccgctcgt gttgacggtg cggatcaatt ccgtgtagtg gccttgggct
     3121 tcggtcaggc ccgcgttggt ccacgccgtt ttttccgcgc tggaccactc gcgcaggcgc
     3181 acgcggatgg tggccgtccc gccagtcggc aggttggcat aggtgaagcg ccaagcctgc
     3241 ggataatcgc ggttcatgtc gctgggcgtg acacgcgcgg ccttctgcca tgcggcatag
     3301 acgttgccat tgccattgga tttgctagtg gcgctgtcgt cgttgctggc gtcgctgtct
     3361 tcaatgcggt accagacctc gcgcacggtt gggtccgtac gcaccaccac gccgtattcc
     3421 gtgccgccga gcgtgtcgcc gttggcggcg ggccactgga catacccctg cgggcgctgg
     3481 gcatccagat agaacgtctg cttgaaggtg ttatataccg cgctgccatc atcccgattg
     3541 aggaatgcgc gcgcggaaat caagtggaag ccctccttga ggccggtgcg cgtctcgcca
     3601 tagtcgttgt gtgggcgatt ggtaatggtg cccagattca gattggtcac ctgccaaacg
     3661 cccatcatgt cggctttgcg cgtaatatcg gtggcgctgc ccggccagac gatctcccag
     3721 ccgttgccat catagccctg ctcgcgcgcg gaacctgttt tgtagcggaa cacggtgccc
     3781 gccggcaggg ccggaacttg cgcctcccac cagtcggtgc tgttggtgcc atcgccgacc
     3841 caattgcatt ccaccacgag cgtatcgcgg ttgcccgccg cgccgcccgc cccctccggc
     3901 caggccacgc cattggtggt gtaatagagg tgcacacgat tgccacccac cttcggcgtc
     3961 ttgatgccga tatagagcgt cgaacccgcg gcattggtca acggcggcca gaattgggca
     4021 tttcccacac gcgggccatc ctgcatggct tgaggattgt ggtaaatcca tgaaaccatc
     4081 tgatcgtcgc tccaatcatt ggagccggag gagggattga tggtccaatt ggcttggcca
     4141 atagtggcca cataggtctc cgcgcccgcc gagcccaatt tgcagcggtt ggaatcggcc
     4201 gcggcaaact tttcgggcca gatgcgcttc acaaaggtgg cctgttcgta gcccagaaat
     4261 acatcgttgg ccagcgccgg tggattgtcg cgcgggtcgc cgccgatgtg attggtgttg
     4321 ttgaggttca tgccgccatc cagcttcatc agcacgttgc cggcggaacc atccacccgc
     4381 gtcgcaaagc tcacgctgct ggcgttggtc acccgcggga tgtcaatcga gtaggaaaaa
     4441 tcggtggtgt tggtatctgg caggccgtag ggattgaacg ccggatcgcc gtcggggcca
     4501 tccttgcgcg tgaccctgac actgcccgct atcttgccgt tttcataaat cgtgatgggc
     4561 ttgccgccga agctcgacca cagcccgctg ggatccggat tcttgtagcc gaaaataaag
     4621 taaccacccg ggggaaccac cactgtattc agcgcggaag cgtaggtgta aaaaccatag
     4681 tagtaatcgg agtagttgaa gaggtaggca tcggaaccgt aaggcccgcc ggcctgatgc
     4741 ggatagctga tgctgctgcg gatgtcgcgt gcctgcccgt tggagtagtc atcgttcagc
     4801 atcacgagca tggtcacgtg ttgcgcggca ttggaaccgg cctgccgcca atccaaacgc
     4861 tcccacgcca caaagtcgcc atcgctccac accccgcgct gatagccgcg ggcaaattgc
     4921 tcgtggcaat agagcaggtt cggcacacgg ttgtcgcccc actgcccgag gaacgatgtg
     4981 ttggcccagc gcgggaatgc cccgccactt tcaccgagcg tttcggcatg gaagttgccg
     5041 tccgtataga gcaggcccag tccctcgcgc aggaagtaga acgcgtgttg cagctcgcgg
     5101 cgcgcggcgt aatcgttgtc atggctttgc gcgtgcatca cgcccaaatt gggcgagaac
     5161 ccgccccacc ccggattatc aaaaccctcc aagccgttcc acggactgcc gaggcgatcg
     5221 ttgagctggt tgcgcaggtc gttgtccacc agccgcattc ccgccgacca atagccgccg
     5281 tagcccggtg gctgtccgag gtgctcgcca aaaatcagcg cgtcatcgcg cggaatttca
     5341 gtattgaaat tgctgtcgcg gtggttgtcc cagtcgctga agccgcgcgt catgttgaat
     5401 tgccactgga tgttgccgca gtagccgagg ttgctgtcgt tcttgtcgaa ctcgccataa
     5461 aaatagtccg gcacgtgctt caccgcgtcc aggcgcaggc cgtcgagctt ggtcatggcc
     5521 atttcccagc gcaccgcgcg cagcagatac gcgccaacgt cctctttgaa gaagtccgga
     5581 tattgcgcga tgatgttggt ggtcagtccg ttgttggtgc caaaaccaat gtagatattg
     5641 gcgttggtgt tgcccgacca atcccacaca ttccattgcg cgggcggcac gttggtgttg
     5701 ggcatccggt catagaattc cggattgtgc gggtggcgca catagctcgg cttgggattc
     5761 cagtcgccct cgttgaggcc gtggttttgg ttgggcgttt cgtgggcaat gtcaatcagg
     5821 tccgacaacc ccaaatgcat cacctgccac gcgtcgttcc aatcgcggca gttgtcccac
     5881 ttgcggtaaa acccgtcttc cgtgcggcgc aggtgaaaat cttccgggca catgccggga
     5941 tacacatcca ccggtgtcgt ctcgttatag ccggggatgt cgaacgcgcg gtggttcatg
     6001 atgttgtcag catagacccg caggccgaac cggtgcgcgg tttcgatcag gttgagcagc
     6061 tccgcttcgg tgccgtagcg cgttgccgtg gtgctgcgct ggtgccgccc gcccaagtcg
     6121 aacggatccc acaagtcata gcccacggaa aggccgccac tacccttggt cggcggtggg
     6181 atccaaatcg aggaatatcc ggcttcggcc agctccggga ttttgtccgt catttcccgc
     6241 cagcttgtgt tgaagtactg cagcatggcc tcgccgtagg ccattcccgg caggagcaat
     6301 cccagcgcgc ccacgccgat gattctccgc caccatttgc gccccaactg ttgtcctgat
     6361 ttcatccctg cactcctcaa cgaactgcaa ccggccattt gcccctacag acaaacaaag
     6421 gcaatcgtta cattaccgac cgactctacc ccaccgtgct cggcaactca acgcaaaaac
     6481 gcttcaccag accataaaaa taagcccgcc ccttcccccc tcctcaagcg tggaccaccg
     6541 gcagatgcgc ccagtccgtc ggccggttca cggatttacg ttgctgccgc atttgccacc
     6601 gggtgctgac caattcgcgg gcgtggcaca ccacgcgccg cgccggctcc cggttcagtg
     6661 tgtccacgca cgtcatcaag cgcatggttg cggggctgtc cggcgtttgc gaggcaaaca
     6721 gcagcccctg ttctgctccc gccggcgtca aatccatgag caacaccccg gccttctgat
     6781 agcgcaatcc cggccggaaa atccgcgtca gcccctcctg cgcgcactgg accaaccggc
     6841 cggaatcctg cgtgggccgc tccaaaacca gcgtgactcc attggcatac cacggttccg
     6901 cgtcggcaaa cggactcgtc tgcacaaaaa ccgtcaccgc ctgcgcccgc aaccgctccc
     6961 gacgcaactt cacccccgcc cgcgccgcga acgccgccac cgcgccttgc aaatctgtca
     7021 gcgaactcaa ccgctcgcca aaactgcgcg acaccatgat ctgttttttg gcgggcggga
     7081 ccaactccaa cggcaggcac gagcaccccg ccagctcgcg gccaatccgc tccaacacca
     7141 cgccgcccaa ccgccgcagc caactgggat ccgcctcggc cagcgccagc gccgtcgcca
     7201 cccccgcctg ccgcagtcgc cccccccagc gcgcagcgat tccccaaatg tcctccaccg
     7261 ccagcgcggc cagcgcctcc cgcggcgcgg ccacctcccc ccagtggcac accggcccct
     7321 gcccagcctg cccttttttg gcgaggcggt tcgccacttt cgccagcgtt ttggtcggcg
     7381 caattcccac cgaaactgga atgcccgtcc agcgccgaac cgtacgcgca atcgcctgcc
     7441 ccagcgccgt cagatcgcca cgatgaccgc ccacatccag aaaacattcg tcaatggagt
     7501 acacctccag ccgcggactg aactgcgtaa gggtttgcat gacgcgccgc gaaaactcgc
     7561 catagagcgc gtaattcgaa gaaaacacca ccacccctgc cccgcgcacc gccggcgcca
     7621 ctttgaacca cggcttgccc atgggaatgc ccagcgcctt ggcttccgcg ctgcgcgcca
     7681 ccacgcagcc atcgttgttc gacagcacca caatcggccg cccctccagc gccggttgga
     7741 acacccgctc gcaggacgcg taaaaattat tgcaatccac cagcgcgaac acgccccacc
     7801 tcaatacgac cggatggcgt gcgccaccac gccccagatc accagctccg tgtgctcatg
     7861 caccacaatt ggcggatacg ccggattctc cgcctccaac cggattccct cgcgccccac
     7921 ccgcagccgc ttcactgtca attcgccatc caccaccgcc accacaatcg cccccgacgc
     7981 tgcttccctt gcacggtcca ccaccagcaa atcaccatcg cggatacccg cccgcaccat
     8041 ggagtcgccc gtcacccgca ccacgaaagt cgccgccgga tgtggcgcca gataggcatt
     8101 caaatccagc ggcccctcca gatggtcgtc cgccggcgag ggaaatcccg ccgcgatccg
     8161 cgaggaaaac aaccgcagca tccgccgcgg ctgtcccacc gccacccgcc acaccgtctc
     8221 cgcaattccc aaggttgttg tacccgcttt catactgaac aattgttcag tatcatacac
     8281 aaccccaccc ccttggcaag cccgggaatg ggggggggag ttagggtgcg attgagcccg
     8341 gccgggtacc tttgcagtca ggataagccg aacagcccca gaaatcgccc ttggccgact
     8401 ggcggcggcg catcggttgt ccgcactgcg ggcaggcagg cgccgccgca tctttttgtt
     8461 ggtcccgaat ctcgcggcgc ttggtggaca gccgctcgct aaagccaccc gttttctcga
     8521 aggccttgcc ctgcgactca atcaagcttt tcaacatatt caacgcccgg ccaatcatca
     8581 tcagcatggc gttggccacc accaccggat catcggcatc cagccaccgc gcatatttct
     8641 tgcgctgttc ccgcgcgtgc ttggcggact catggaccat gtcatcctgg aatgcggggg
     8701 catccagcga aaccgcgttg atggccttgg cctccggcga atgcaccgac cacggcaatt
     8761 ccccacggtc gagaatgaaa acctcatagt cgccgcgcaa ttcgctcaaa ctggcgcgag
     8821 ccacatccgt cagtttcatt tcggtgtctt tggaggtggc cgcccgttcc gagccttcaa
     8881 taatgttctg ccgtccgccg cgggcggctt gaaccatttg atcatattgc cgacccttgg
     8941 ggtcatagaa cttggtttcc cgttcgcgcg ggtcggccgt caggaagcgc cggcaaaacc
     9001 gcagcgtttc caactgcaca atggtggcca gcgtgaacga gtgcaaccgc cgaaacccac
     9061 catgcttgtc aaacagttcc gccatggcac cacctatgct gtcgtgacct cattgaccac
     9121 attgtccggc atcctacacc aaacaatacg gtcaataaca acaatcttgt ccatgtccgt
     9181 ccatttccgt ccccaccgcc gcccccgccc cccctccggg gcgtggcttg cgtggcgggc
     9241 ggggggcggc tacaatccgc gcccatgaaa actaaaactt cctggctgtc gtccgcggtg
     9301 atttaccaag tgaacctgcg ctcgttggcg gcgcgcgagc cgcgcaacgc cgtcgaggcg
     9361 ctgaccgaaa agccgcaacc tctctccccc ctggcctacc tgaccaaatc gttgccgcgg
     9421 ctgcggcggc tgggattcaa cgtggtctat ttgctgccgc cctaccccat cgggctcctc
     9481 ggccgcaagg gaatcggctc gccgtatgcc tcgcgcgatt tccgcgctgt cgaaccggaa
     9541 tacggcacca tggaggacat ccaggcattc atccgccgcg cgcacgagct gaagctgaaa
     9601 gtgattttcg acatcacgcc caaccacact gcgcgcgacc acgtctggat gaccgctcac
     9661 cccgagtatt acgtcaaggc cccggacggt tccgccttct ttgatgccga ttggtcggac
     9721 accgccaagc tggactacac caatcacgac ctgcgcgcgg cgatgatcga ggtgtacgac
     9781 ttctggcttt cctgcctcgg cccggatgag cacggcgcgc cggatggcgt ggacggcttc
     9841 cgcctcgaca tggcgcactt catcaatgac aagtcgttct gggaccaagc gctgcccgcg
     9901 ctgcgcgcgc ggcacccttc ccgcgacttg cttttcatgg ccgaatgcta cggcttccag
     9961 aacaacctcg atcttttcgc ccgcggcatg gatgcggcct atgacgacga tttctacaag
    10021 ctgtgccagt atgcctacgc cgtggatgaa gccggcaact cgcgcgtgcg tcttgccgcg
    10081 gatgcccagc acaaccacga tttccatccc atcctcaacc agtggcagga tggcggccta
    10141 gcggcggcgg cagccaaagt gttgcgccaa tacgaggagg ccgcagcccg ttttccctgc
    10201 cggcattaca ccgcgcgcta caccgacaac cacgatgagg gccgcggcct gtaccgcttt
    10261 ggccccggcg cgttccgggc catgaacatt ctcgcgtttc tggcgccgcg cacgctgccc
    10321 ttcctgctga caggccagga atccggtgcg ctcaaccgcc cctccatcca cgcgcgctgc
    10381 caaccctgcg acaaaggccg gcgcatcctc actgccgatg gccgcgaagt ccggcaggaa
    10441 ggcgtggaat tggaaggcaa cctgttcgat cgcggctacg ccgcgcgcca gcagtggcag
    10501 cagtttttta aggagctgat cgccctgcgc aaaaaacacc gtcccctgcg cgaaggctcg
    10561 tgcgacatcc tcgatcttgg cgaggatgcc ggcccccgcg accgcaccgt gctggcgttc
    10621 gaccgcaaac tcggccagca actgctgcgc tgtgtgatca atcttggtga ccagccacgc
    10681 cacttggccc gcgccaacgc cttgctcaac ggcacgccgc tctatggcac actgccggcg
    10741 gcgggcgtgc tgccgccgtt ttcggtggtg gtggtcagca tcgcgtaacg ctgcgccggc
    10801 gcaaaaaaca ggccgcggcg cccatcgggc ccgcggcctg ccgtgccaag caaccggatc
    10861 aggtggtcac ttggattttg cgcgccttga cttcctcgcg cttgggcagc gtgacggtca
    10921 aggttccgtt tttgatgcgc gcctggatgc gttcggcgtc caccgtctcg ggcagggtga
    10981 aatcgcggcg gtagcggcca gcacggaact cgcacccgag ctcttcgccg ggcgtcgggc
    11041 aaccgcccgt ctggcaccag ccttcaatag tcaacacccg gttatgcacc gtggcctcgg
    11101 cggtcttttc gtcggcgccg ggcatatcag ccaacaccgt gaagcgttcg gcatcttcgc
    11161 ggatatccac gcgcgggata tacaccggct cattggccgg cacgggggca tcttggcgtt
    11221 tttctgtggc agtcgtcatg gcttcactcc tttcattccg ccgtgatggc gattttacgc
    11281 ggtttttcct ctggcgagcg gggcacggca atgttcaaca agccgttctt gaaggtggcc
    11341 ttcaccccgg ctgcatgggc acggaagggc agttgcaacg tgcgctggaa ctcacccgcg
    11401 ggccgttccc ggcgcaagac ggtttccccg gcggccggcg ccgcgctgtt gaccttgccg
    11461 cgcaaggcca gctcgtcgcc gtccacggag atttctacat cctgcggatc aacaccgggc
    11521 atttcagcca ccagcgtcaa tccatcggcc gtcgaataca cattcaacgg cggatacgcc
    11581 gcgccagcgc gccgctccac ccggggttct tgtcccgcca acaggcggtt catgttgtcc
    11641 tggagcgatt ccaattcgtc gaaaatagtc cagggactac ggggtccaat catcagttct
    11701 aacatggcga gcactccttt cttggttcgg ttgagttgct tgtccacctt gtacagagca
    11761 gaccccatgc caatttttct ctagtgcaat ttaattaatg caacaaactg caaatgaata
    11821 agaactgact ttttaatcag acggatattt caagcttgcc agataaaaaa gcgccatttt
    11881 gtcgctcatc acgcgacaca atggcactac cccccccccc cnnnnnnnnn nttgcgactt
    11941 atttcgctgg gggaatgacc agcaccgggc attcggcttt gcgcacaact cgctcggtga
    12001 cgttgccaaa cagaatcttg ccgaccgccc ccgccccctg ccgacccaga ataatcaggt
    12061 caatgccttg ctcttgggca tactcaagaa ttttttgccg ggcctcgcca atgcgcatgg
    12121 cgatgatcag cttgacctcc ggcggcacgt gcggctgata ggcctcggcc agtttggcat
    12181 caatctcgcg cttggcccgc gcgtccacgt cctcgtccac ttcgtagata tagcccttcc
    12241 aaaactgggc gggcggttcg gggatcacgt ggagcagatg cagctcacag ccgggattgc
    12301 gctgggcggc gcgtaccgca aacacgaacg cgcggtcggc attcgcggaa aaatcggtgc
    12361 aaaacagcat cttgcgatat tgctgctgcc aaagtttgtc caccatggtc gcctccttgc
    12421 ctgccctacc ggacccatgt gacgggcacc gtcaccaact gcggaaacac caacaagatg
    12481 agcgcgccca caatcaatgc cagcaaaaac ggcagcgcgc ccttgaagac actggccagc
    12541 ggcacggccc ggtccatgcc gctcaccaca tacacattca cgcccaccgg cggcgaaatt
    12601 acgcccatct gggtcaccag cacgatcacc acaccgaacc aaatcggatc aaatcccagc
    12661 ttgagcgcca ccggataaaa aatgggaatg gtcaacatga ccagcgccag cgcgtcgaga
    12721 aacgcgcccg ccaccagata aaacccgatg atcagcagca tggtccccca ccccggcagc
    12781 ggcaggttgg ccatccactg agccaacgat gtcggcacat tggtgatttg caaaaagtgg
    12841 ccgaataccg tcgccccggc cacaatcacc agcaccatgc aggaggtgcg cagggtttcg
    12901 ttgagcgcgg tggccagcat tcgccacgtc agtttgcgcg ccagtgcggc aatcacgaga
    12961 ctgcccgcgg cgccgatggc cgcagcctcg gtgggcgtga accagccggc aaacattccg
    13021 cccatcacca gcgcaaagag cgccaggatt tccaccacgc cggacagcgc gcggaagcgg
    13081 gcgcgccaca gctccgcggt ggccggcggc gccagcgcag gccgccagcg gcacaggagc
    13141 atgatggtca gcatgaacag cgcggccacc agcaaccccg gcacgatgcc ggcaatgaac
    13201 aacttgccga tgctttgctc cgtcatcacg ccatacacga tgaagacaat gctcggcgga
    13261 atcatcatgc cgatgctccc gccggccgcc acggtgcccg ccgcaaaacc agcgtggtac
    13321 ttgtagcgct tcatttccgg cagcgccacg gcggccatgg tggcggcggt ggccgggccg
    13381 gagccgcaaa tggcgccgaa tgccgtgcac gcgcccaccg tggccatggc cagcccgccg
    13441 cgcagccaac caaaccagct ctgggccgcc gcaaacaggc gctggctgat tcccgtgtgg
    13501 aacgccacct gccccatcag cacaaacagc ggaataaccg tcaggccgta gttggaaaac
    13561 gtgtcgtaat aatccgtgac caggatatag agcgcggcgt tgccgttgac catcacggcg
    13621 tagcccacca cgcccacaag cgtcatggca aaagccaccg gcatgctggt ggccagcagc
    13681 aaaaagagca gcgctattcc gcacaaaccg acttgcaggt ccgtcatggc ttacctcggc
    13741 cgcaacaggg aacgccccgg atggagcaga tgccagagcg tcacgcccac cgtcaccaca
    13801 caggccgccg ccgcgagcca cggcacccag aacatcggca tgtgcagcgt ggtggtcacc
    13861 tcgccgctct cgaaaaacgc gttgccctgc agcacaaact gccacgccag caggctgaag
    13921 agcagcagca gcaccagccg catcagcgta tccgtcacgg cgcggccgcg cgggcgcaac
    13981 ttcaggaaaa aatattcgat ggcaatgtgg cccttgacgg ctttggtcag cggcagggcg
    14041 cacaccatgg cgatggcacc caagacgcgc accagatcat acgcgccacg cactggcaac
    14101 ccgcagcgcc gaccaatgac gtcggccacg gtcaccagaa taattcccag cacggcgagc
    14161 ccgcccacca cggccatgcc gtggatgacc cgccgcagaa aaccggtgcg cgcgctggcg
    14221 ccggacaacg gggacatgat tacggctggc tctccgccgc cgcgagccgg gtttggatgg
    14281 ccgccagcat ttccgcgccg ggcaggccct tggcggttgc ccgctgcaca tagtcagcca
    14341 ggatcggttc catggccgct ttccagccgg ccacttcctc cggcggcagc gtaatcactt
    14401 cgcggccgag atcgcgcaca aacgcccagc cttcctcatc ggcatcgttc caagcctgcc
    14461 catgtttgtc cacccattcg gcgcttaccg tggtgatgat ctcctgcaaa tcggtgggca
    14521 gcgcatccca cgacttctgg ttcatggcca cgaacatcgc cgtggtgtag ccgatggcct
    14581 tggtgtcggt gatgtacgca atcacttcgc cctgcttcca ccccttcagc gtctccaccg
    14641 ggcagaaggt gccctccacc acgccctttt gcagcgcgtc atacgtttcc ggctgcggca
    14701 tgcccaccgc agtggcgccc agcgccgtgg caatgccggc ggaaagaccc gtgccgcgga
    14761 ttttcatgcc cttcaaatcg gccagagtcc gcaccggttt tttggtggcc aggatgccgg
    14821 ggccgtgcgc gtggacgtag agcaacttgg cgccttggat ttccgccgga gcaaactgcg
    14881 cggccagctc ggtggccatg cgcgtggcgg tcaggccatc gggccaaccc agcggcaagt
    14941 ccagcgcctc cagcagcgga aagcgcccgg gggtataggc aaagcagctc atgccgagat
    15001 cggaaatgcc gttcagcacg ccctgccagc actgatctgc cttggtcagc gtgccgccgg
    15061 gatacaccgt gatttgcaac cggccgcccg agcgtttttc cacttccctg gcccattcgg
    15121 tggcggtttg cgcctggatg tgcatcggcg ggaaaaacac gctgtaggtc agcttgtagg
    15181 tcttgtccgc tgccgcatcc ccgcccgccg catcgcgctt gccgcagccg ctcagcgcca
    15241 ggcccagcgc cacgaatccc actcccgcga gtcccagata ttttttcatg cccactcctt
    15301 tgttcgccat ttccagcggg tcgtcatcct atcgccaatc cgcagcgccg cgcaactctt
    15361 tccgccggcg cgcgacaaaa tcggttgacg acgcccgcgc gctcttgtat ctacatacag
    15421 aaacatcgca acgaacccct tggaggcatc atgaacgtcg ctgattattc caactacttc
    15481 aacctgcacc ccgacaagga aggccgcttc ggcaaatatg gcggcgccta cctgccgccg
    15541 gagctggaaa aaatcatgga ggaaatccgt gatgcctacg acaccatttc gcggtcggcg
    15601 cggttcatcg cggagctgcg ctccatccgc aagcatttcc aagggcggcc cacaccggtc
    15661 tatcacgcgg aacgactttc ccgcaaactc ggcgcggcgc aaatttacct caagcgcgaa
    15721 gacctcaacc acaccggcgc gcacaagctc aaccactgca tgggcgaagg cctcctggcc
    15781 acctatctgg gcaaaaagaa actgatcgcc gagaccggcg cgggacaaca cggcgtggca
    15841 ttggccaccg ccgcggccta ctttggcctc gagtgcgaca tccacatggg cgaggtggac
    15901 atagtcaagc aagcccccaa cgtcgcgcgc atgaagctgc tcggcgccaa tgtcatcccg
    15961 gtgaccttcg gcctgcgcac gctcaaggaa gcggtggatt cctgctttag cgcgtatctg
    16021 aaggaccccg tcacccagct ctattgcatc ggctccgtgg tcggccccca ccccttcccg
    16081 atgatggtcc gcgacttcca gcgcgtggtc ggcattgagg cccgcgagca attccacgac
    16141 atgaccggcg acatcccgga catcgtcacc gcctgcgtgg gcggcggctc caacgccatg
    16201 ggcttgttct ccggcttcct cgccgacccg tgcgaaatcc acggcgtgga acccctcggc
    16261 cgtggcccca aggtgggcga tcatgccgcc accatgacct acggcaagga aggcgttctg
    16321 cacggtttca attcctactt gctgcaagac gagaagggcg agcccttgcc ggtgtactcc
    16381 attgcgtcgg gattggatta tccgtcggtg ggtccggaac actcgttcct gaaagatatc
    16441 ggccgcgtgc aataccacac catcactgac cgcgaagcgg tggaggcatt cttcatgctt
    16501 tcgcgtgtgg agggcatcat ccctgcgctg gaaagctgcc acgccgtggc gcacgccatc
    16561 cggctttcgc gcaccgcgcc gaagcactcg cgcagcatcc tcgtcaactt gtccggccgc
    16621 ggcgacaagg acatggacta cgtgatcgag cactacggct tcggcgaaaa cttcaagctg
    16681 gactgatccc gccgcgcgcg ggcggccaat caaggctctt caaggcggcc cacgttgggt
    16741 ccgcattggg acacccaaca atttcgttga ttttgtcggg tttttgtagg gcgggaatgg
    16801 ttgagggacg aaaccttccc gccggaaaag gctggaaggt ctgcgaccgt tccagccttc
    16861 aatccaaccc gaatcgcgac actcacgaag gacatggact acgtgatcga gcactacggc
    16921 ttcggcgaaa acttcaagct gaactgatcc cgccgcgcgc gggcagccat cagatatctt
    16981 cgaggcggcc cagcagccgt agcaatccat ccagaatggt gatggggtgc ggcgggcagc
    17041 ccgggatgaa caaatccacc ggcaacaggg cggttgcgcc attgcgcact tccgcatggc
    17101 cggcgaatgg gccgcccgaa atggcgcacg cgcccacggc aatcacaagg cgcggctcgg
    17161 ccagcgcgtc gtaggttttt tgcagcgcca gcgccatgtt ttcggtggcg ggcccggtga
    17221 tgagcaggcc atcggcatgg cgcggcgatg ccacaaagcg gatgccgaag cggcttaaat
    17281 cgaacaccac cgtagtcagc acgttggtgt cggcctcgca agcgttgcag ccgccggcgc
    17341 taacctgccg caacttgagc gagcgcccga acaggctttt tatcttggcg tcgagggcga
    17401 cggccaacgg gcgttcacgc ccggtcacca ccaaatcttc gcgtcggcgc acggccatcc
    17461 gatggtcgtg cgtcaaccgg atggcgccgg tggggcacgc ggcttggcag tcgccgcaaa
    17521 acaggcaacg gcccatgtcc agttgcagca cgccattgac gcgggtaatg gcgccggtgg
    17581 ggcacgccgc gacgcattgc gtgcagtcgg cgcggcattt gtccgggttg agttccggcg
    17641 cgccacgcag ccggtcgggc aacgcggggg gctgcgccgg gtaggccacg gtgcgatgtt
    17701 tttgccgcca gcgttcgaga atcagccggt tcataggtcg tgcccgcagt aggaaaggtt
    17761 gaagctcttg ttgcagaggg ggaaatcgga aatttgctgg ttgcgcagcg ccatggccag
    17821 ccccagccag ttgtggaacg aggggtccac cactttgtag cgggcaaaat ggccggcgga
    17881 atccgtggtg gccatgtgcg cgatttcgcc acgccagcct tcttgcagcg aaaccaccgt
    17941 ggcttctggt tgcagcgcgc cgcagggttc atggcacggg ccgggcggca aagtggtggc
    18001 cagcgtccga accagcgcca cggattgttg aatttcctgc cagcgtactt gggcgcgagc
    18061 gaggacatcg ccatcttcgc ccagtgcagg ggtgatcgtc atgccttggt aggcgcctgt
    18121 ggggaaatcg cgccgcacgt cgcgcgcgca accgctggcg cgagcagccg gccccaccaa
    18181 gcccagttct tcggccagcg gcttggccac ggcgccggtg ttctcgaagc gcgccatgac
    18241 ggaggcggaa tcccagagca ggttgaccgc gccggcggtg tcgcggaacg tggtcgccag
    18301 ccgcgtcagc aaggtgtcca agcgcgcctg atccacgtca aacatcacgc cgccgggacg
    18361 caccaggccg cgcccgaagc ggctgccgca cagcagcgcc gtcatgttca gataatcgcc
    18421 gcgcaagcgc ccgcagtagg acgccgtggg cagaaaaccg acatcgccgg ccagcgcgcc
    18481 gaggtcgccg gtgtggttgg ccaagcgttc cagttccagc gcgatggcgc gcaatgcacg
    18541 ggcccgcgcg ggcaccacgc agccggccag cgcctccacc gcctggcagt acgccacggc
    18601 atggccgatg gtggtatcgc ccgcgagcgt ttccatgtgg tggatggtca gcggatgcgg
    18661 cccgtccagc agcgcgcgct cgatgccgcg gtgctgatag cccaacgaaa tttccagatg
    18721 cagcacttcc tcgccgtcgc actggaagcg gaagtggccc ggctcgatga ttcccgcatg
    18781 cacggggcca acggctactt catgaatgcc ctcgcccgcc acacggaaaa actccatgac
    18841 tgatggcgcc agcggcgcat cgccgcgccc ccaggcatcg cgtccggggc gataggcgcg
    18901 gtgaaaacgc agcggcttca accacgggtg cccctcggga atcacggccc attgctccgc
    18961 gatttcgcgc tcgaagccgg ccgcttgtgg gcaatccggc gtcagcgcgg gataggattc
    19021 gcccgcgaga gccgccagca ggcgcaaacc gccgctgcgg tcatccgcca gcgcggccac
    19081 caggcgcaca ttgccggcag tggccggcgt gccggccagc acgctcaacc gctggccggc
    19141 ggcaatgttt tccaggattt cctgccggaa aactttcatc ggcaacaggg gaatccggtc
    19201 gttggcgatg ggtgccagat ggcgcgtgag caagctcatg gcacccctcc ccccgcccag
    19261 aggctggcct gcgccagcaa gcggtgcaag ccgttgggca gccagaggcc caacgccagc
    19321 gacaacgcca acagcatcag cgatggccca taggcgcgcc gcgcctcgcg cgcgggggca
    19381 agcccggccg gcgcgtcgcc atacaccatg ttcaggaaag cgcgcgacat tccaacaaac
    19441 gccacgccca gtgccaccag cgcggccagc gcccacccac cctgcccctg cgcgaaggca
    19501 gcgcgcagca tggtgaactc gcccgcgaac gtgccgaagg gcggcatgcc gctaatggcc
    19561 atcaatccgg ccagccacag cgcaccgctc actggcagca accgccccac gcccgacacc
    19621 ttggcgatgc tcttggtttg gtagagcgca accaggttgc ccgccacaag gaagagcgca
    19681 gctttcacca gcgagtggtt caacgcgtgg agcagcgcgc catacatggc cacgccgccc
    19741 acgccggtgc cgaatgccag cagccccatg tgctccacac tggaataagc cagcatccgc
    19801 ttgaagtcgc tttggttcac gatcagcgcg gcggcgaggc ccatcgagag caggccgaag
    19861 ccggtcaaca gcgcgccgct aaattcggcg agccccgccg cggcgcagat ggcgtgcgcg
    19921 cgccaaatgc cgaggaacgc gcaattgagc agcgcaccgg acagcagcgc ggataccggc
    19981 gagggcgcct cgctgtgcgc gtccggcagc catgtgtgca tcggcaccag gcccattttg
    20041 gtgccgtagc ccaccagcag gaacacaaac gccgccttca gccatgccgg ttgcagaccc
    20101 acgctgcgcg ccagcagttc cggcaccagc aacgtgccgc cggtgccatc ggccgccgcc
    20161 gcggcaatca ccacgaggaa gttgcccagc aacgccagcg caatgccaac tgaacacacc
    20221 agcagatatt tccacgtggc ctccagcgaa ctgggctgcc ggtggaaata aatcagcggc
    20281 gcggtagcca gcgtggtggc ttccaccgcc acccacagca cgcccaagtg gcggctgacc
    20341 gtcaccagcg tcatcgtgga caaaaatacc agcaggcacg cgatgaatac cgcctccggc
    20401 gcattcacga aaacggtgcc ggtatccact tccacctgcc gcccctggct gccgcgtgtt
    20461 tggcgcaggc tgtgcaccgc atagaccgcc accgccaaaa acagaaagct tgtcagcccc
    20521 aaaaacagca gcccagccgg atccagcccg agccaggcgt cagcggcgca ggccggcgga
    20581 cggatgaagg ccagcacgct caacgcggca tggagggcag ccgtggccac cagcagcgcg
    20641 tggcgctgcc ccggcgcgcg cagcagcatg gcggccaccg ccgccgccaa cggcaaaagc
    20701 accagcaatc ggatcatggc gcatcctccc gcagcgcggc caactgatcc gtgttgatgt
    20761 ggtcaaactc gcgattgatg ttgaacacga tgatgcccat gacgaacacc gccacaaaaa
    20821 tatccagcag cacgcccagt tcgaccagca gcggcatggc ctccaccagc acgatgccca
    20881 gcgtgaaaat gccgttttcc agcacgatga accccagcac ctgcgtgatg gctttgcgcc
    20941 gcgccacaat catgaacagc cccgtccaga cggtgaacag gcccgccggc acgagtagat
    21001 cagccaccgg cggccacggc agcggcaggc gcgcgccaag ccagaacgac aacgccaacg
    21061 cccccacccc gcacagcagc gatagattga agccgatgaa cggctccatc tcgcggtggc
    21121 cgtgggtttc gcgcagcgcg cgccgcaaca tggccggaaa aagcagcccc ttgatcagcg
    21181 cgctgcctgc gccgagcagt accgccgccc acagcatccc gccatgatgc gccagtatcg
    21241 gcagcacacc cagcccaatc ccctgcaacg cgacaatccg gatggcgttg tgcagccggc
    21301 tcgcgccaag caacacgagg ttggtcagca cgagggcaat caaaagggtt tcaatggcat
    21361 tcaccgcatc accagaacgt caacaaaagg cccaaggtgg aaagcacccc cgcgccaacc
    21421 agcaactgcg gcacccgcag cagccgtacg cgcgccatca ccgactccac caatcccgtc
    21481 aggattgcca acaccaccaa ccacaggaag ccgacagcca aatccagcaa cgggcagccg
    21541 gtggctgccg ccgggaacag acccaccagc agcgcgccca ccacccacag cttgagcgcc
    21601 gagccccaca ggataaacgc gtagtccacg ttgccgtgat ccagaaccat gacctcgtgg
    21661 atcatggtca actcgaggtg cgtcgccgga tcgtccaccg gaatgcggca gttttcggcc
    21721 aaaaataaaa taaaaaagcc ggagccgagg agcgccatgg cgggcatgag cgcggctggc
    21781 cagtcggcat agatgccggt cagcgagaga tcgccggagg cgtgggccag cgcggccatg
    21841 ccgatcaaca gcgccggttc ggtgaacagg gcaaactgga cctcgcggct ggcacccatg
    21901 ccctcgaagc tcgaaccggt atccagcgcg gcggccacgg tggcaaagcg cgccagtccc
    21961 agcaggtagg ccagcagcac caaatcgccg gcgaacgaca ccgccgctgg cacgcggccc
    22021 agcggcagga tcaacagcgc caccagcaga cacacgaaat tgagccgcgg cccggcgcgg
    22081 aacacccacg tggtggtgcg gctatagacc gcgcccttgc gcagcaattt ccacaagtcg
    22141 taatacaact ggagcagcgg cgggccttcg cggcccgcca tgcgcgcctt gacgcgattg
    22201 attacgccgg gcagcaacgg cgccagcagc aacgccgcca acggatgcaa gatggccgcc
    22261 gcgttcatga ccacagcgcc catcccaaca gcgccagcag cgccaccacg atgtacaaca
    22321 cataaaagtg cacccgcccc tcctgcaccc aacggaacca gcccagtccc gcgcccaccc
    22381 agcggaacag cggatcaaaa aagacgcggc gggccacatc gggaacttcc gccgtccgac
    22441 tgaccgcggc cgggaaaatc ccgacgggat cctgctgccg gctttgcgcc tgcaagatgg
    22501 cctgcgcgcc gtgagtcaag ggctgcacaa aactggcgcc ggtatattgc atgctgggcg
    22561 cgggcgcggc gtagccgcaa tcccatgtga cagtctcgcg gacgctgcgc gcggccaaca
    22621 gccgccgccg cacccaagcc atcgccagcg cgccggcgat caagacgagg aaaagcccgc
    22681 tcaataccgc cggcacacgg gtcgcggcca gcagtgcggc atcggtgccc gccagttgcg
    22741 tcaccaccgg cacgaggccg cgcaccacat gcaccgcgcc cacgcccagc gccaggcaaa
    22801 gccccgccag cagcaggatg ggcacggtca gcgaagccgc ggcctcggtg gcgccggccg
    22861 ccgccggcgt gcgcggctct ccttgaaaga ccacgccaaa cgccttggta aaacacgcca
    22921 gcgccaaacc gccgatcaac gccaaactca ccaccaccac caaaccgccc aatgccacgc
    22981 cgccggtggc cagcagcgct tgaaatgagg ccacaaaaat caggaattcg ctgacaaacc
    23041 catttaaagg cggcagcgcg cagatggccg ccgcgccggc aaagaaggcc agccccgtca
    23101 acggcatccg tttcagcagg ccgccaagtt gttccatgtt gcgcgtgccg gtggcctgcg
    23161 ccaccgcccc cgcgcccatg aacagcaggc ccttgaacag cgcgtggttc agcacgtgca
    23221 gcagggcgcc cgcaaaaccc aaggccgcca ccgccggcga ctgataatgc agcccgagca
    23281 gccccaaccc cagccccagc gccacaatcc ccatgttttc gccgctgcta tacgccagca
    23341 gccgtttcaa atcgcgctgc gccagcgcga acaaaatccc cgggatgccc gacaccgcgc
    23401 caatgcccag caaaacccac gcgccccacg ccggcacctc gcccagaatc agcagcatcc
    23461 gcacgaggcc atagatgccg gttttgatca gcacgccgga catcaccgcg gataccgggc
    23521 tgggcgctgc cgggtgcgcc tccggcagcc acacgtgcag cgggaaaaaa ccgaccttcg
    23581 cgccgaagcc caacaacgcc agcgtgaaca aaagccccgc cgcgccgccc gcgcgccacg
    23641 tggcaaaatc ctgcgagcct tccgcgcgcc ccagcaaggc gaatagcgca atcaaaaatg
    23701 ccgcgcccag atgcgtggcc accagataaa tccagccggc ctcgcgggag ctggcggtgt
    23761 cgtccatgac caccaggaaa aacgaggcca ggctcatgat ttcccacgcc accagaaaca
    23821 gcacgccgtt gcgcgccaga atcaccaatg ccatgctggc gacaagcgcg ttgtagaaaa
    23881 accagaatcg ccccggattg cgcccacccc acgtcttgaa atagccgccg gcataaatcg
    23941 ccgccaagcc gcagagcagg aaaagcggca gcaggaaaaa tgaactcagc ggatcaagcc
    24001 ccaacgccag ctcgccggcc ggtgtccgcc agggcagtac gatgtggcct tcgaccgcgc
    24061 gcgtcaaacc ggccagcgcc acaaagaaac ccaccacgca cgccagcacc gcgccgagtt
    24121 gcccagccgc cgccgcgcgg cgctccgccc accggccata ccacaacgcc gcggcgccag
    24181 taatcggcag gatgccgatg gcccacagca agacaaaaag caggatggta gcgtcgtgat
    24241 tcatcagtaa gggacaaaag aagccggaaa caagacactc atggttgtac ccggttgggc
    24301 ggacgagtca agctccaaca cgtagcgcgc caaggtgaaa ggccaaccgg aatgcaccgg
    24361 tcagggggcg tcggcggagg gcgggggcaa atcgggccgg cgcgcccggg tgcgggcgat
    24421 ggcctgctcg cgccgccact tggcaatctc cgcgtggttg ccgctggtca ggatttccgg
    24481 aatgcccagc ccgcgataaa ccggcgggcg ggtgtattgc ggatattcca acaggccagt
    24541 cgtgaacgat tcatcatcgg ccgcgccctc accgcccagc gcgccgggca ggagccgcac
    24601 cacggcatcc accaccacgg ccgcggcgag cacgccgttg gtcagcacgt agtcgccgat
    24661 ggaaatttcc atgtcggcca gcgcggtgcg gatgcgctca tccacgccct cataatgacc
    24721 gcaaataaaa atcaagtgcg cggcctgggc caacgcctgc gccgtggcct gcttgaaagg
    24781 ctcgccctgc ggcgtcatca gaatgacttt ggattccggc cgccgcaaat ggtccaccgc
    24841 cttgaacacg ggctcgggct tcatcaccat gccggggccg ccgccgtaag gacggtcatc
    24901 ggtggtcagg tgcgcgccag tggcaaaatc gcgcaggttc acgcagcgga tggtcaccaa
    24961 gccggcttcc tgtgcccgct tcaaaatact ttcccccagt atgccgtcga gcatccgggg
    25021 gaaaatggtg atgatatcaa tgtgcagcgg agccgccgcc atgggggaag cctattcgac
    25081 cacttccagc atggcgcgcc ggttctgccg cgacgccacc gtgctgagta gggtgcggat
    25141 ggcgccaacg gttttgccgt ttttgccgat cactttgccg acatcctcgg gatggcagcg
    25201 tagctcaaac acaatcgtgc gctcgccttc gatttcggtc aggcgcagct cgtcgggatg
    25261 atccaccagc gctttaacga cgtattcaat caattctttc atggggaacc tcggggatgg
    25321 agatgaggaa gaggaatggg agggggaaaa ccgcggcgcg cgcgcttatt gggcggcggc
    25381 cttgcggctc ttcttgatca gcgcggcgac agtttcgctg ggctgtgcgc ccttggccaa
    25441 ccaggcttcg gccttggcga ggtccaactg gtagttgttg ctcttcttgg cggggtcgta
    25501 ccagccgagt tcttcgagga aattacccgc gttggcgctc cggacatccg tggccacaat
    25561 gcggaaaaac ggcttcttgt tggcgcccat ccggcggaga cgaatcttaa ccatgttgtg
    25621 tatccttgtt ctttcgagtt gtgccgctcc cgggcgcggc caatccattt tgaggacccg
    25681 gggttgttca aatcggcgtg ggttctacct gaaaagccct ccgcgaggca atcttttttg
    25741 caggcgttgc atccgcttgg ccatttgccg ggcatcgcgg aagttgcgca gaagggtatt
    25801 gacatcgctg acgtgcacgc cggcgccctt ggccacgcgc ttgcgccggc tgccgttgag
    25861 caggtccggg tgccgccgtt cgcgcggggt catgctcagc acaatggcct caaacttttt
    25921 catctggtca ccggaatcat tctcaatgcg attgcggatg tcgcccgcga tggcccccgc
    25981 ccccggtagc atctccatca atttgcccag tgagcccatc tttttgatct gccgcatctg
    26041 ggccaaaaag tcgttcaggt cgaggccttc cttgcttttg aacttggcct ggattttttc
    26101 ggcctcggcc acgtccatgg tttcctggac tttttccacc aggctgacca cgtcgcccat
    26161 gcccagaatc cgcgaggcca tgcggtcggg gtggaacacc tcgaggtcct cggggcgctc
    26221 gccggtgccc acgcgcaaaa tcgggcagcc ggtgacggcc accaccgaga gcgccgcgcc
    26281 gccacgcgta tcgccatcga gcttggtcaa aattaatccc gtcaggccga gggcgtcgtg
    26341 gaaggtcttg gccacattga cggactcctg cccgatggcc gcatccagca ccagcaccac
    26401 attctgcggc tcggtggctg cgcgcagcgc ttgcagttcc gccaccagtt cttcatcaat
    26461 ttggaaccgg ccgccggtgt cgtagagcac aatgtcgtaa taattttgcc gggcaaattg
    26521 gaaagcgcgg cggcccaggt ccggcacgct ctcgccaggc tgcggcttga cgattcccgc
    26581 acccgcctgc ttggccagca cttccagttg gtccactgcc gcgggccgac gaatatcgca
    26641 agccaccagc agcaccttct tgcccttttg cttccacagg cgcgccagct tgccgctggt
    26701 ggtggtcttg ccggagccgt gcaggcctag gagcatcact tccgccggga tggcgttcag
    26761 gttgaaatcg cgatgctcgc cgcccagcag cgccaccagt tcatcgtgga tggccttgat
    26821 gatctgctgc cccggcgcca cccccgccag caccgtggcg cccaggctct tggccttgac
    26881 ccgctcaatg aattccttgg ccgtggcgta atggacatcc gcttccagca gcgccagccg
    26941 cacttcgcgc agggcgtcct gcacgttttt ttcggacaac tgccccaccc cgcgcagatt
    27001 gcggaaagcc gtttgcaggc gactggtcag gcgatcgaac atgatcgaat cagggcgcag
    27061 gttccgccgg gcgcaccaag ccgcgaccgc cacagcgggg gcaggtgccc acctcgtcgg
    27121 tatcgggcag cgtggtgcga cccatgccgg cgcagagcgg gcaaatccgg tccaaatcat
    27181 cctgccggcg aatcagccgc gagcccacgc cgagacaaat cgggcaaatc gcgcgctcgc
    27241 cggtttcgct cagcacgctg ccggtgccga ggcagttgtt gcactccagc gtctcgacgc
    27301 cgggcgccag gttggcgccg cccgccgccg cgccgccgcg cgtttccagc atccagttgc
    27361 ccagttggtt catgaacgaa ttgggcgcaa tgttctccgc ccccgtccgc cgaacttgct
    27421 gcatccattt gagctgccag ataaacccgg caatcaccac ccccgccatc agcacaatgt
    27481 acacattgcg atacttgaac tgcgccggtg gcagccgcgg cgtattcacc gcgggtttct
    27541 cgctgcgatc acgacgcgcc aatttccggc gtagtcggat ttgcggctta acaaactgag
    27601 ttgggtctgt gcgcacagta gctccggaac aaccaccgcc tgatcttccg cgatttccag
    27661 cagcagcttt ccgccggatg taagcacacg cgcggcctcc gagacaagcc gtgaaatcag
    27721 ccgcaaaccg tccacgccgc catccagcgc cagatgcggc tcaaagtcgc gcacggtgcg
    27781 cgcaaggcgc gcgcaatcgg cggtgctcac atagggcggg ttggcaatca ccatgtccat
    27841 gctgtcgtcg gcaaagccgg tcagtaaatc cgtttgccgg aactccaccg ccgcgccgag
    27901 ggcgcgggcg ttttcgcgcg ccagatcgag cgcatcggcg ctgatatcgg tgccccacac
    27961 ccgcgcatgc ggcacgcgct tggccagcgc gcaggcaatg cagccgctgc cggtgcacac
    28021 atccaccacg cgcggcgcgg gcaattcgcg caagaaatct tcggcatggt cggttagcac
    28081 ctcggtttcg ggccggggaa tcagcgcgcg ccggtcgcag cggatgcgca ggcccatgaa
    28141 gtcggtttcg ccgatcacat attgcaccgg ctcgtgcgtg gccaaccgct ccaccgccgc
    28201 ggcgaaggct gccgcgtgtt cgggcgtggg gatttccgcg gcatgacgga acacatccgc
    28261 caacccgcag ccgagcaggt gcgccgccac ccagcgcaat ttggtgtccg cctcctccac
    28321 ggcggccgcg cgcaaccgac gttccgccgc cgcgagcaga gccgaccacg gctgcggtcc
    28381 cgtcatccct tggccccgtt gatttgctcg gccagccgca gctccacatc gcgggcttgc
    28441 agcgcgccga tcagctcttc caacttgcct tccatgatct gatccagcga gtacagcgtg
    28501 aggttgatgc ggtggtcggt cagccggttt tgcggaaaat tatacgtgcg gatgcgctcg
    28561 ctgcggtcgc cggaaccgat ctgcgttttg cgctcgctgg tgcgtttgtc gtgctcctgc
    28621 tgccgcttca ggtccagcag ccgcgcggcc agcacgcgca tggccttgtc gcgattgcgg
    28681 ttttggctgc gctcgtcctg gcacgccacc accagccccg taggcaggtg ggtaatgcgg
    28741 atggccgatt cggttttgtt gaccttctgg ccgccgggcc ccgacgcgca aaacagatca
    28801 atccgcaggt cctcgggctt gatttcgacc tcgttgattt cttccaccgc cggcagcaca
    28861 gccacggtcg ccgccgacgt atgcacccgt ccctgcgcct cggtttccgg cacgcgctgc
    28921 acgcggtgcc cgccgctctc gtagcgcagc gttttataga cgtccgtgcc ctcgacagaa
    28981 aaaataattt ccttgaagcc gcccaaatcc gagtgcgccg catccatcac gctggttttc
    29041 caaccttggc tgtcggcaaa cttggaatac atgcggaaca agtcgccggc aaacagcgcg
    29101 gcctcttcgc cgccggtgcc cgcgcggatt tccatgatgg tgttgcggct gtcgtccgga
    29161 tccggcggca gcatgagcgc catcaacttg cgctcctggc gggacagcgc ggcggtcagc
    29221 tcctcttttt cggcctgcgc gatttccgcc agctcgggat cgccagcggc ttcggccagc
    29281 agcgcggcgt tgtccgccag caaccgttcg agacgccggt attcctccgc cgccgcattc
    29341 agctcgcgca ggtgctgatg ctcgcgcacc agctccttga aggtctgggg atgcgacgcc
    29401 gcgccggggg ccgccatcgc ctgttcgagt tcctcgaggc gcgtggccac ttgcttgaaa
    29461 tagagcggat ccaattccat gagcgggcag gtcaaagccg ggacagaaaa cgggccggac
    29521 tcgttcccgt cctccgggaa ccatccggcc cgcttcgtcg ccagctactt cttggctgtc
    29581 gccggcttgc gctgataacg cttgttgaac cgatccacgc ggccttcagt gtcaatgaac
    29641 ttggcctgcc ccgtgaaata cggatggcag ttcgcgcagg tgttgatgtt catgctcttg
    29701 acggtggagc gggtcttcag cacatacccg cacgcgcagg aaatggtcgc ttcgtcatac
    29761 ttgggatgga tatctttttt catggtcttt tccggggttc cgggctcacg cacccggatg
    29821 ttgccggatg caaaacgcgc ggaacctatc accgccccac ccccttggca agcgacaatt
    29881 tcgccacgcc gcgccgattc cgcttggatt gttggccgga acggtcgctg accttccagc
    29941 ttcggttcga ttgtagggc
//
LOCUS       DAOPNP010000013        28980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2087_length_28980_cov_10.157753, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000013 DAOPNP010000000
VERSION     DAOPNP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28980
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2087_length_28980_cov_10.157753"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PKI64_02305"
     CDS             complement(<1..1560)
                     /locus_tag="PKI64_02305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOD99872.1"
                     /translation="MTPQAILKYPVVFLSGLLVALVLAPLWRRWAPGWGFLDRPDDRK
                     IHHAPVPRGGGIVIFIGFHVACAVLFLAPWKPFAGQLTAIWWLRFLPLSAGVILLGLL
                     DDRFNLKPVTKLAGQILLAMAAYASGIHLQNIFGYAFPAWVDAVATVIWFVALMNAFN
                     LIDGVDGLATGIALIAAAGIGLSLVFRSAPGDVLIFLGLVGACLGFLRYNFYPASLFL
                     GDAGSLFLGFTLAALSIPTNAKASFMAGLGVPMLAVGVPLFDTLLAIWRRSVRRLLTP
                     TTGAGPLPRLDAADTDHLHHRLLRQGRGQGQVAGLLYLATLLLVVIGLLATVFNDRAL
                     GILALAFLLAAYTIVRHLAGIELRESGRVVLQGLARPVRRNRTLILYIAGDLLILNAA
                     LIIGHLLLSAYAGQATPDLKTTWLRHFPTDVVLPFLVLLLFRSYSRVWYLARVSEYAA
                     TGFAVVLGYAAAAGLHLLTKQTHAGILALGLHYLLLAGMAAPAVVFTRAALRVVQDLM
                     QRRPRDTAGTQP"
     gene            complement(1557..2750)
                     /locus_tag="PKI64_02310"
     CDS             complement(1557..2750)
                     /locus_tag="PKI64_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD99873.1"
                     /translation="MKVILLNQFFPPAQAPTGILLRDAAVALAQRGHTVVVLTSAAGY
                     GATGAAADLPENVSVVRLGTAGQHGSRPRGKLRDYRDFYRAAAGELQRQQPADALVCL
                     TTPPFIGLLGARWRQKNGLPYILWCMDLYPEVLAAHGWLRPWNPLQFILRRWARRERR
                     GAAAVVALGPDMAARLRNTGAARVEMIPVWASTRLNPQVEAAARALRKTRGWADDEIV
                     LLYSGNMGRAHSATEFAALAEHLRQVSPRCRFVFAGDGPARAAWARRWSDRFEFMPAV
                     PGDAAAHLRAADVHLVSQKPAWQGIVVPSKFPAACAVGRPVVFAGPPQSAIGQWLGAA
                     DAGWLLNEPAPADIVAVARALCDPAQRTAKGQNALRLHQARFTPEDNCARLVALVEET
                     AQKNP"
     gene            complement(2747..3577)
                     /locus_tag="PKI64_02315"
     CDS             complement(2747..3577)
                     /locus_tag="PKI64_02315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD99874.1"
                     /translation="MNDPLPISVVVLSHNEAANLPRCLRALRGCAVVVVVDDGSTDDS
                     QRIAAELGARVVVHPFASFADQRNWALAEAGLRHEWTLHLDADEVVTPELLDEIRRRI
                     PTLVPGNVGQLARKIMLGERWLKHSADYPVYVPRLIHRQGPRFVMRGHGETIAADPAA
                     SVYFTEPLLHYSFSKGWAEWRARHQRYALAEATRIRSGAAEVSWRALVSRDTATRRAA
                     ARALSYRLPCRPMLRFVYAYILRLGFLDGAAGWRFCLEMAGYERMINAALRQPVPPPP
                     "
     gene            complement(3594..3965)
                     /locus_tag="PKI64_02320"
     CDS             complement(3594..3965)
                     /locus_tag="PKI64_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99875.1"
                     /translation="MPSAQIWAPWNLGVGDYATISHGVDVYNVARIEIGAHATVSQRA
                     FLCAATHEVDHPNMPLKTAPIRVGAGAWICAEAYLHPGITVGVDAVVGVRAVALHDVP
                     PGQIVGGNPARFLRQRNVAAG"
     gene            complement(4159..5238)
                     /locus_tag="PKI64_02325"
     CDS             complement(4159..5238)
                     /locus_tag="PKI64_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOD99876.1"
                     /translation="MKCVVWVRTAPPDQAGSMAAYADLVQRSLAPLAADWTFATCDLF
                     DPRGGASMWRHHVWRLRHARRALTAHPADLYHWLDGSMAAFIPPALRARSVVTVHDLI
                     PLLQARGELPRRPSWPARWLIQRGVTALRECALLCADSAATKADLARLAKITARVKVV
                     PVPLRPLPPPRPVALPGLPPGARFILHIGHNASYKNRRGVVDVFLRLRDSADLHLVLA
                     GPSPSPELVARTNTLPMVHFVGPVDDAELHDLYRRAALFLFPSLYEGYGMPILEAMAA
                     GCPVVCSTAPSLLEVAGDAALSAPATDPDALADACRQLLSDAALRQTMIERGRARAAG
                     FDIASFGNLLLKCYEQALTPKEPHD"
     gene            complement(5235..6584)
                     /locus_tag="PKI64_02330"
     CDS             complement(5235..6584)
                     /locus_tag="PKI64_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99877.1"
                     /translation="MLLVALATLVALVNWRWGIGAAIVLGLLQDPLRKIIPGTPAYLV
                     LAGMPIWLGVICSAAFSRQLPINRFLSQFPRLGLWIRTFAIYLVIPAAISATYGHNSW
                     LITLLGIFIYSTIFGIFVAGWRYPHRSEDIARLLTLYAAVTALMLMGGPLDAFKLPYL
                     QAFVGTDMFGAIWVTHRTGEALHMLSGFFRSPDVMGWHAALLFMVATVLALRSKGVVR
                     WLWVAVAIWGIFNIWLCGRRKMLSMIPIFLGTYTLLVFRFKNARRIFSLVGMAAMIMG
                     LGWYVIASVVHNEALERFYVSTLAEMGERVETHGLLSVAGTIAQSGFWGYGLGMSQQG
                     VHNIPNVETPRLWQESGPSKIVAEFGVPGMMLYLMVGVVLFITAYHVVRFGRERDSFY
                     LTAGIFAILVANVASAVVSAQIFGDPLVAFLLAFIAGLLLSGARLAAQHAPRTEGAP"
     gene            complement(6593..7816)
                     /locus_tag="PKI64_02335"
     CDS             complement(6593..7816)
                     /locus_tag="PKI64_02335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD99878.1"
                     /translation="MTAPASGQAMGYGRAPHRLVIVETHPIQYKAPLFRKLAAVPELD
                     VTVLYAMEPSPVEQGAGFGASFAWDVPLRTGYRSELLENRARHPSVTTFRGCDTPGIA
                     RRLRELRPDAVLVNGWVAKTCLQALRACRRLGIPCLVRGEANLLRPRAAWKHLLHRLL
                     LRQYAGYLAIGSANREFYRFHGCPEERILWAPYVVDNDFFAAQAAARTARRAEIRAAL
                     GVAPQATVFLFCGKLQPKKRPLDLLRAVGQLPADCRARAQVLVAGDGELRAECERVAR
                     ELGVATAFAGFINQSRLPEVYAAADVLVLPSDAGETWGLVVNEAMASGRPAVVSRAVG
                     CAADLVLPGQTGEVFELGDTAALAGILTRYLREPALAARQGTAASRHIQSFSYQQVIA
                     GILTAVQTCARGARC"
     gene            complement(7813..8748)
                     /locus_tag="PKI64_02340"
     CDS             complement(7813..8748)
                     /locus_tag="PKI64_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD99879.1"
                     /translation="MSAQTSPVPFSIAIPTYGRDEVLVRTVAQLLAQQPPAAEILVLD
                     QTVAHEPATNRQLQQWHTAGAIRWVRLTEPSQPAALNHGIREATQEIILCLDDDIEVD
                     PSFVGAHAQCYADVSVVGVAGQVLQPGQTPRKNYRQATTADPFGDLHFDFSSATATWV
                     RNGMSGNLSFRREAALAIGGFDENFLPPVSYRFDAEFCARLCRAGGRIRFEPTASIRH
                     LRAARGGTRASGSHLTSASPVHGVGDYYFALRSGRGWPRLRYLLRRPFREVCTKFHLA
                     HPWWIPVKFIGELRALVLALRLHRQGPRLLGDTAP"
     gene            complement(8745..9632)
                     /locus_tag="PKI64_02345"
     CDS             complement(8745..9632)
                     /locus_tag="PKI64_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOD99880.1"
                     /translation="MAWRWSWLRLVSWVYRFRTLPRAERAFAGAPSPCILRRQLFGFS
                     MAVDVSRAAVQRLLFLEGERFIGERFLLSRLVSKGDTAVDVGANIGYYLLLLARLVGS
                     EGKVIAVEPSRENLPELKRNIAENKLSNVELHEVAAGSERGTVGFMSGINGRITAATA
                     AVDQVPLVPLDDIISTRVDFLKMDVEGAEGRALAGAQELVRRWHPVIFVEIHPHMLGA
                     FGDTTRGVMKWLRAEYDSVEIYENVESRLSAWGKIAVRYACHDPVHRIENPDEFLATM
                     DVNPPACTFWAVAQNDKKR"
     gene            complement(9636..10190)
                     /locus_tag="PKI64_02350"
     CDS             complement(9636..10190)
                     /locus_tag="PKI64_02350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOD99881.1"
                     /translation="MIARWIYRWREFIRIALARGRVYLYRWQGADIHPKCLLGRGTRL
                     EHPWTIHLGARCMLQPNVWLNVGDPNASLQIGEYTFIGRGTEIEVSLRVTIGKGALIA
                     PGVFITDHNHSLKRGAPMFQQRGEEAPVTIGDDVWIGANAVILCGVTIGEGAVVAAGA
                     VVNRDVAPYSIVGGVPAKPIGQRT"
     gene            complement(10187..11371)
                     /locus_tag="PKI64_02355"
     CDS             complement(10187..11371)
                     /locus_tag="PKI64_02355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD99882.1"
                     /translation="MASPVALRILFAADLVRNPDSGAAGTEWQTLAALRRRGHHIDEI
                     WGDDLPHRIRHWNLHYIFELPRAYEAAIAAAYRRQPYDVVHINQTHCWLAAREHLRRR
                     RPGVVVQRSHGWEPRAEEVIRRWLRASGRRTRRGWRSIPGWWVDHRLGQHARWALRHA
                     AGTIVSSTLDRDYIMSTYGTPGEKVACIAQAPAPIFQEAPPAPWNPARLNRLLHVGGY
                     SAAKDCAAVSQAASTLLELDETATFTWVLPAADCAAALATLPSAVRPRAQAIPWMPQE
                     ELVRIYDEHGVFLFPSLFEGFGKVFLEAMARGLCVVGTPTGGMRDLIRPGENGALVPF
                     HSPADIVSAVRHWWANPATAACISAAAVATATAYSWDSVAAETELFYTQLLSQSAGRR
                     GS"
     gene            complement(11364..12365)
                     /locus_tag="PKI64_02360"
     CDS             complement(11364..12365)
                     /locus_tag="PKI64_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOD99883.1"
                     /translation="MSTPAISVVMGVFNEAAVLAPTLDSILAQTERDFELIVVNDGST
                     DPRVGEILAEYARRDARLRVLTQANAGLTRALIAGCAAARGTYIARMDAGDVMTPDRL
                     ARQKAVLAAHPEVVLVTCWTEFCGPAWEPLFVERGTQSSATAGDWIAQATTTAAEGYL
                     VNGPSSHPSVMFRTAAYRAAGGYRWQFYYGQDGDLWVRLADHGAFAGIAAVLCRSRIF
                     PDGISMRQGKKQRRLHDCLFGTIQARRSGASEEPYLQAAAQIRPQPSGSQTTTSRRKA
                     AGWYFIGETLRRNGDPRAVTYLREACCRQPWQVKHWLRLWQSVRLANRPLPAGERHG"
     gene            complement(12362..13384)
                     /locus_tag="PKI64_02365"
     CDS             complement(12362..13384)
                     /locus_tag="PKI64_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99884.1"
                     /translation="MIWLVVPTAWEGLPGAGQWKDLHGIRLVLTRLGNEWREFRFHED
                     NISELIARIGSANDTVIWYYTFWPEAMEELRRCCPQVKIVLRTVNAEARQHWTRAEKQ
                     WWRLRGLPRDIYGFLRLLSRDRRCCRVADVLAGISPWDDRHYWARLAGTTPLVAAPYF
                     CPWPALRPDVTLQPWTQRKTDALCLAGTRDPIGRGHVAGFMALARQPAWAGRGMAASA
                     GLLDAPPDDWPGNITALGRVAEPWPLFGASKALAVLSPLGSGCKTTVTDALAAGCYAL
                     VHPRQHDRLPPNERELAILLEPASAVDAARALAAITQPPAYAAGEIQQAQFNRAVNAW
                     REVLAS"
     gene            complement(13381..14385)
                     /locus_tag="PKI64_02370"
     CDS             complement(13381..14385)
                     /locus_tag="PKI64_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HOD99885.1"
                     /translation="MNPPTLKQKLGCWWHERWRLELVLRAPDLARRAGLEWLDLCNRH
                     ERLAKSPDSHGRICLWSDSSLLTVCRIFPRTGARLLRHVWSTDHEPPTAPLSIILPVR
                     GIERAPVVAFVMETVRRQIGADSEILLCEHDTAPRYAHLAVAEIRHVFVPAVADEPFN
                     KSKAMNAGARAARHCTLVLLDADGVLPPTFLARAMDCLVRGWEAVRPLRFLFLLDEPA
                     SHQFVHTGRVDGVRQVAAVQQNTPGMVTVIRRDSYAALGGHDERFAGWGGEDLEFLDR
                     LQTRKLYPGSFLPAIHLWHSPAPQKASGHRNHELMLRIMAEPTANRVAQARQRWERGS
                     "
     gene            complement(14382..15344)
                     /locus_tag="PKI64_02375"
     CDS             complement(14382..15344)
                     /locus_tag="PKI64_02375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD99886.1"
                     /translation="MKTPTAMPSPAPPLPPLPANPLISVVVPSYNQGAFIRQTIESCL
                     AQDYRPFEIIVMDGASTDNTVQVLQSFGQRPELQWVSEKDDGVVDAVNKGLRRARGEI
                     CAIQSSDDYYLPGAFSHAAATFARQPAAGLVFGNVDRVDVAGQLLFSPRQPAYSLARL
                     LAREVFVYQAAAFFRRDLALQLGGWNPRIPHVPDTDLWYRLAFHAEVVQLRSALAACR
                     THPGQRDTHLVAIYDEYLQMLAESRELVTAPRVLRRAAAAGAALLKFRYGGPWSDAEL
                     TRAAWRALCYRPSLLWSPALPKHRLLPGYFALTRLRRRLLGRPP"
     gene            complement(15341..16351)
                     /locus_tag="PKI64_02380"
     CDS             complement(15341..16351)
                     /locus_tag="PKI64_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HOD99887.1"
                     /translation="MTAPALIAVITTIQPPTPAVRELNRWLGEVGGRLVIAGDKKGPA
                     AYDLPHTDFLSLAAQQASGFALAELLPTGHYARKNIGYLHAIRAGAPCIYETDDDNAP
                     LPAWAPRTETVAAPRTVAGAGWINAYRYFSADRNIWPRGFPLEQLAADPAPASATAAS
                     FRAPVQQGLVNGSPDVDAVWRLTQNRPFDFDPAAPLALTAGQWCPFNTQSTWWWPAAY
                     PLLYVPSHCSFRMCDIWKSFVAQRCLWALGAGVVFHAPEVVQERNPHDYLRDFHDEVP
                     GYLQNTRIADILTGLDLAAGEAAVADNLLACYDALIREKIFPPAEWPLVQAWIKDCAA
                     PA"
     gene            complement(16348..17607)
                     /locus_tag="PKI64_02385"
     CDS             complement(16348..17607)
                     /locus_tag="PKI64_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99888.1"
                     /translation="MSTPVIEVAQLGKTFQLGEIHRDWTRRAADRLRGHRSPPAPILQ
                     ALREVSFTVPQGEVFGIIGANGAGKSTLLKILAGITDPTTGKATLRGRIASLLEVGTG
                     FHPELTGRENVFLNGTLLGLRRHEIVEEYDRIVEFAGLETFMDTPVKRYSSGMQVRLA
                     FAVAAHLRPAILLIDEVLAVGDVTFQRKCLGAMKEVAGGGRTVLFVSHNMAAVSSLCQ
                     RALMLRQGQVAAIGAADEVIQTYIHSTEEQQTRDALGFVLLPERKTPRPPEPGLICAA
                     RVVSAAGQPAASVRTGDSLRVDVRVAGLDRFPAAVVGLTVRSVFDQKVFHFNSAMAGG
                     FRAVKRQPHEIVRLEVADVPLMSGAYSVDVLVFEPGVRLLDRVDGALPLDVIDSDIYG
                     FGYRGEAREGLIAVRGACAIAPGPESP"
     gene            complement(17604..18440)
                     /locus_tag="PKI64_02390"
     CDS             complement(17604..18440)
                     /locus_tag="PKI64_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99889.1"
                     /translation="MAAREVMYVRRPRTGWFDLALDELWAYRDLLFTLMWRNLTVRYK
                     QSAIGIAWVILKPVLQMLVFSVLFGRLANLPSDGVPYPIFLYAGLLPWTYFATTITSG
                     TGSLLAGSNMISKVYFPRMILPLSYLFTSLIDLALSLSVLLGMMVWYRADMVFGPQMA
                     LFPLFLVPAIALAGGLSLWLSAIAVRYRDIHHLVPFLLQIGMFVTPVVYAVNLLPESY
                     QWLLQLNPMTGVVEGFRWALLGKSVSGWGIMGAGFGLAFLILLTGMIFFRRVERTVAD
                     VI"
     gene            complement(18445..19758)
                     /locus_tag="PKI64_02395"
     CDS             complement(18445..19758)
                     /locus_tag="PKI64_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD99890.1"
                     /translation="MKRAASIVLLPLLVLLLTAVAWPAAQPAPWSTALVPALTLLLLL
                     ACVITQPPPLRLWAAGVGLLALLYAYVWNPSHSWSSTAGLVPQDYHTGWPAAAYPPGA
                     RAALLFAGTVWTAFVAGVSLSRRQLSWLPALAALATAGMALVVISQRLEPNAPRIREF
                     TGIFVNENHYAVFANLLLPVVLAGAARARFRAVQAGLPSSPAGLFYLAAALSAAGVVM
                     SKSRAGVAVMALLVGAHVLLHWRTVRRYPFAGVPLSPVFKALGVAAFLTAVAFAVRAF
                     WAEWQQVSAIASEWTYRSGILRHSFTAWLESPRWGYGPGSFAIIFPYYQAAEYARHTI
                     LHAHCEPMQFLVEYGWAGAAILLALTTLAFSARCAPPATDPAEVPAFADCERCAFGLA
                     LLAGALHCLVDFPLRIPLLALLIALWAGVWVAARPCSDPNPRPEA"
     gene            complement(19755..21899)
                     /locus_tag="PKI64_02400"
     CDS             complement(19755..21899)
                     /locus_tag="PKI64_02400"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOD99891.1"
                     /translation="MQKYPYLADVADNANETSRFPDWREAVAILVERAWLGGLIFAVI
                     LLFFIFQAKRQTPYYRSTATLMVEAQVPRILNYQDVLAINTRSLEYFNTHITALHSRK
                     VLEAALEQGGFFDRPGFFSNLPRQAVVDAASGLVEIAPIERSRLINIKVEHSVPEIAS
                     DLANAIAKAYIQQDLDSRMQTTIEAVEWLRERSEEYRERLEKGLLELQAYREQTGSVS
                     LENDQNIVIEKLKALNSALTMAQTERIQAETQWHAVKSQMDKKVEPAKIATVLHDESL
                     VLVSQKLQEQQRVVAALQQRYREQHPELQQALELEQKLQGQFTQAYSNAIYALKNNYQ
                     TLVQREQNLRAALAAQEKESFELDRKLVRYNELKRNVEADQEIHQAVIARMKETSISE
                     SLPVDIIRLAEEARPAKAPFRPQVRQAIFRGIVFGLIGGVGAIFLLYYADHRFRRNEE
                     VERLLGLPVLATIPLIRDKTVEERGMVAHLKQTGEVAEAFRTLRASLLLQDELKQAPV
                     LMFTSAGPGEGKSLTSTNLAITLAQDGRRTLLIGADLRRPLLARIVGSSRSALGLADA
                     LRGDCVWTDVLLKQVAPNLDVLLSGRIPTHPAELLGNPRMVEILNEARQTYQHVLLDA
                     PPVLGISDALVLLPHVDGVLFVVRYGVTHSLGASHALRRIQSGGTTCLGAIMNGVNLQ
                     SLATYYYYRRYGGYAYQQYQSQPAEPPAPVAE"
     gene            complement(21916..22644)
                     /locus_tag="PKI64_02405"
     CDS             complement(21916..22644)
                     /locus_tag="PKI64_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOD99892.1"
                     /translation="MKTRTWQRGCRWLLLAGLVASGVAGCKSPPTTSPPPGALVLPAP
                     APKKSGKAAKVAKTPKATEAPAADTTTAPVPEHVLQPGDEIDVQIYREPDLSGTFRLS
                     PAGEIRHSLAGTIPLAGKTITEAEGELTRRLNEDFLVNPRVIIKLISTQSSQIVLLGE
                     VKKPGVYPLAYGETITLLQAVAGAGGFTELASPDRVRIVRRGPDGQQVTIPVRVSDLL
                     KGKRGQRDIPLEPNDVIMVPEVLF"
     gene            complement(22699..23982)
                     /locus_tag="PKI64_02410"
     CDS             complement(22699..23982)
                     /locus_tag="PKI64_02410"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD99893.1"
                     /translation="MIRLRLVSSLLLAAAGFAPLARAAGDALANGFYTRGVEMAAYMD
                     QGSPVGDPFYIPAPTGALLPRVTLTVTHEDNVFLDPGEKTEGTSVRLVPGVLGLWGRP
                     ADNHVYVDYGVSIPVYDSAPDVDDNPAHMLKLGVVYRTGKSQVQGQVGYHRQEDLDVV
                     AGARLAKQDYLADLSLEHRISGKSSLGLVGRAERHDFEADRYLDYDRYYGAGRLYHRA
                     TAKSEVFAQGGIGRDDPLRDSDARNGADFYDLSLGVRGKQSPKFHTTGRVGYMWRAYD
                     DPERADAGDWIAALTAESSPFGLATFRGELYADIRPAIDAHGVDTVDQGGTLSVERRL
                     FIERLRGNASLTAGRVEYSGSPHADENEWVRDGRTDNYWGFSVGVDWWTRQRFSVGLA
                     YSYMRRDGSRGAGAEAEDATSYDYGRWTLRASWNY"
     gene            complement(23979..25898)
                     /locus_tag="PKI64_02415"
     CDS             complement(23979..25898)
                     /locus_tag="PKI64_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOD99894.1"
                     /translation="MMVQQKVAQARRKTRLVFRVAGWAAALGWLWGVPAAPAAPASGE
                     AIVAAMTGHAEARVFTAPVHEGRTDLRQLFEGAALHENSRVITGKDGRLCMVMSPGAI
                     VCVAPQSQFQIEQLRHTGDGLPRTAEDIIRRIHLNLERGRIRVHAGAPLPTLDIRVVT
                     PAGVIEAHGGEFVVAQGESGQWLVFTHAGELTLTPRGGEPVTLGAGNNARLTLAGERA
                     ELQTTGVPNDPARESFELCNVFFSDLEPFIEHPLDFDREGLGNYLGLNAPFQDVDDGA
                     LVTDASPTIRPAVAGAQPTRLPRAGENAPGARWGERRIWDWYNRLGVVKGVNYIPRTA
                     VNSVEMWQEATFDPDTIDQELGWAHDVGYTSIRVQLQFAVWQADPDGFLKRVDKLLEL
                     AAKHGLRVVPVLFDDLDVAGRAPVSGPQPEPVPGEYNSQWVPSPAHAMVTDRQQWPAL
                     EKYVKDVMGRFKRDGRVLYWDLYNTAGNSGLWEESLPLLDQTFNWARSVEATQPLAVA
                     AWKEFGSAMAARKLERSDLITFQSYDTKEAVEARLMLLQQYKRPIICSGWLMRQSGNQ
                     FDKILPLFAMGHVGWFNQGLVAGRTQLEVQQTGFRSESDPDLWQQNVLREDGTPYDRR
                     EVELIQGFQFVETPE"
     gene            25897..26358
                     /locus_tag="PKI64_02420"
     CDS             25897..26358
                     /locus_tag="PKI64_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOD99895.1"
                     /translation="MALSSFHSPTIIAELLRQQTPVTFRARGPSMKPTIRDGSQLHCL
                     PAPARLPRGSIVLYTFCGRLCAHRLLRHDRHTRRMYVVGDAALTGGRWLPAESVLGVV
                     VAARDERRERPLATRAARWRGLLRHVLRPARRWVHRWRQRLHALTSAKRPS"
     gene            complement(26325..27221)
                     /locus_tag="PKI64_02425"
     CDS             complement(26325..27221)
                     /locus_tag="PKI64_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99896.1"
                     /translation="MKDVGQLWIGPVSFRLEAAPELNVRYSRWAYAGFYVPAPAGAAP
                     VDALTVRIRRGALPPPESEPLWRGGLNWAAWESGAELILCAGFAGRNHARWHCRVDRA
                     LTRAELTVACGAEVCEAPLEYPLDQILTWGLLARAGGVLLHAAVAVHDGRGLVLAGRS
                     GAGKSTLANLCHEAGWQILNDDRVLVYPTADGWRVAGTPWHGSGRFAVADTVPLAGVC
                     LLQQAATEWLEEVPPSMARLALLDVAAVPWFLDDWAQAALDGLDNMLGSVPVRRFHFT
                     KQPAAVAALAELQDGRLAEVSA"
     gene            complement(27218..27400)
                     /locus_tag="PKI64_02430"
     CDS             complement(27218..27400)
                     /locus_tag="PKI64_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99897.1"
                     /translation="MTPNPEPLPVATDLQERRPYTKPEIKRVDLALAETLSTGCKFEN
                     DSSCVGPPITAWEAGS"
     gene            complement(27397..28488)
                     /locus_tag="PKI64_02435"
     CDS             complement(27397..28488)
                     /locus_tag="PKI64_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD99898.1"
                     /translation="MSCPADFTQDWLAAFRARAARTRTPVAVLFELTRRCNLRCVHCY
                     LGDQIAQHRRQAEELSTPAIKEAMAEWVAAGCLTMTLTGGEPLLHPDFAEIYRHAREL
                     GLVVTVFTNGTLVTPETVSLFQEFPPRKVEISLYGATAATHDRVTGQPGSHARAWAGI
                     RRLLAGGVRVELKSVLLTLNRHELDALEAQARECGVPFRHDAAIFPRLADGSPEPLTL
                     RVPPAEAVAADLATPERRSRWREKINRTAELPESDRLYPCTAGQTSFHADPFGGVSPC
                     LLATDFYCAPSARSFARVWAEDLGAIRQCRRTRPDGSLVGPARGACTHCPAVNRLETG
                     APEVESDYARATTRLRHAAAQQAETGDAP"
     gene            complement(28485..28778)
                     /locus_tag="PKI64_02440"
     CDS             complement(28485..28778)
                     /locus_tag="PKI64_02440"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOD99899.1"
                     /translation="MSAGTVYRPVGRVVVRRIGADRLLVPVSGLAAQGNAVFPINETG
                     EFLWTRLAQGLTPAQAADELAAEFAVAPADAQADAEEFAAQLVAAQLLAEADT"
     gene            complement(28775..>28980)
                     /locus_tag="PKI64_02445"
     CDS             complement(28775..>28980)
                     /locus_tag="PKI64_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99900.1"
                     /translation="FPRNGKTLRDFSTQWKKFDGFFHTMENLWGDFSTQWKMFDDFFH
                     TVEKIFPHCGKVSRGRVAAGRAA"
BASE COUNT         5926 a         9628 c         8800 g         4626 t
ORIGIN      
        1 gggttgcgtg ccggccgtat cgcgcgggcg gcgttgcatc aaatcctgca ccacgcgcaa
       61 cgcggcgcgg gtgaacacca ccgcgggcgc ggccattccg gccagcagca ggtagtgcaa
      121 ccccagggcc aagattcccg catgggtttg cttggtcagc aaatgcaggc cggctgcggc
      181 ggcgtagccc agcactacgg caaagccggt cgcggcatac tccgacacgc gcgcgagata
      241 ccacacgcgt gaataggacc gaaacagcag cagcaccaga aacggcagca ccacatccgt
      301 cggaaaatgc cgcagccatg ttgttttgag atccggcgtc gcttggccgg cataagctga
      361 taacaacagg tgaccgatga tcaacgccgc attgagaatc aacaagtcgc cggcgatata
      421 caaaatcagc gtccggttgc ggcgcaccgg gcgcgcgagg ccttgcagga caacccggcc
      481 actttcgcgt aattcaatcc ccgccaagtg gcgcacgatg gtgtaggccg ccagcagaaa
      541 ggccagcgcc aagatgccca aggcgcgatc attgaacacc gtggccaaca agccgatgac
      601 caccaacagc agcgtggcca gatagagcag gccggctact tggccctgcc ccctgccctg
      661 ccgcaacaag cggtggtgca gatgatcggt atcggctgca tcaagccgag gcagcgggcc
      721 ggcgccggtt gttggtgtca acaaacgtcg tacgctgcgc cgccaaatcg ccagcagcgt
      781 gtcaaacagc ggcacgccca ccgccagcat gggcacaccc aaaccggcca tgaaggaagc
      841 cttggcgttg gtggggatgg acagcgcggc cagcgtaaag ccgaggaaca ggctgcccgc
      901 atcgcccaga aaaagactgg ccggataaaa gttataccgc aggaaaccga ggcaggcccc
      961 caccaatccc aagaaaatca gcacatcgcc gggtgcgctg cgaaagacca gcgaaagacc
     1021 gataccggca gcggcaatca aggcaatgcc ggtggccaaa ccatccacgc catcaatcaa
     1081 gttgaaggca ttcatcaggg ccacaaacca gatcacggtg gccacggcat ccacccacgc
     1141 cggaaaagcg taaccaaaaa tgttttgcag atggatgccg gatgcgtaag cggccatggc
     1201 cagcagaatt tgtccggcca gtttcgtgac cggcttaaga ttgaaacggt catccagcaa
     1261 gcccagcagg atcacgcccg cgcttagcgg aagaaaacgc aaccaccaga tggcggtgag
     1321 ctggccggca aacggtttcc acggggccag aaacagcacg gcgcaggcca cgtgaaagcc
     1381 gataaaaatc acgatccccc cgccgcgcgg cacgggggca tggtgaatct tgcgatcatc
     1441 cggccggtcc agaaaacccc agccgggcgc ccagcgccgc cagagtggcg ccaacaccag
     1501 cgcaaccaac aacccggaaa gaaacacaac gggatatttc aggatggctt gcggggtcat
     1561 ggatttttct gggcggtttc ttccaccaag gcgaccagcc tagcacaatt gtcttccggc
     1621 gtgaagcgcg cctgatgcag ccgtaatgcg ttttgccctt tggccgtccg ctgcgcggga
     1681 tcacacagtg cgcgggctac ggcgacgata tcggcgggag ccggctcgtt gagcagccaa
     1741 ccggcatcgg ccgcgcccag ccactggccg atagcgcttt gcggcggccc cgcaaacacc
     1801 accggccgtc cgaccgcgca tgccgccgga aacttgctgg gcaccacgat tccctgccac
     1861 gcgggtttct gggaaaccaa atggacatcc gctgcgcgca aatgcgcggc cgcatcgccg
     1921 ggcaccgccg gcatgaattc gaatcggtcg ctccagcgcc gcgcccacgc cgcgcgtgcg
     1981 gggccatcgc cggcaaacac gaagcggcat cgtggtgata cctgacgcaa gtgttcggcc
     2041 aaagcagcaa actcggtggc cgaatgcgcc cgccccatgt tgccgctata cagcaggaca
     2101 atctcgtcat cggcccaacc gcgggttttc cgcaaggcgc gggcagcggc ctccacttgg
     2161 ggattcaacc gcgtggaggc ccacaccgga atcatctcta cgcgcgcggc ccccgtgttg
     2221 cgcagtcgcg ccgccatgtc gggacccaac gcaacgacag ccgccgcacc gcggcgctcc
     2281 cggcgggccc agcggcgcaa aatgaattgt aaaggattcc aagggcgcaa ccaaccgtgc
     2341 gcggccaaca cttcgggata gaggtccatg caccagagaa tgtagggcaa cccgtttttt
     2401 tgacgccacc gcgcgcccaa caaaccgatg aatggcggtg tggtcagaca gaccaaggca
     2461 tccgccggtt gctgccgttg caactctccc gcggcggcgc ggtaaaaatc gcggtaatct
     2521 cgcagcttgc cgcgcggacg actcccatgc tggccggctg tgcccaaccg caccacgctc
     2581 acattttccg gcaaatcagc cgcggcgcca gtcgcgccgt aaccggcagc ggaggtgagc
     2641 acaaccaccg tatggccgcg ttgcgccagc gcaactgcag catcacgcaa caaaattccg
     2701 gttggcgcct gtgccggcgg aaaaaattga ttgagcagaa tgaccttcat ggcggtggtg
     2761 ggactggttg gcgcagggcg gcattgatca tgcgttcata gccggccatc tcgaggcaaa
     2821 aacgccaccc ggcggcgcca tcaagaaagc cgaggcgcag aatataggca tagacaaagc
     2881 gcaacattgg ccggcagggc aaccggtagg acagcgcgcg agcggcggcg cggcgggtgg
     2941 cggtgtccct cgagaccaat gcgcgccacg acacctcggc ggcgccggag cgaatccgcg
     3001 tcgcttctgc gagggcgtag cgctgatgtc gcgcccgcca ttccgcccaa cctttggaaa
     3061 aactgtagtg cagcaacggt tcagtaaaat aaacagaagc cgcggggtcg gcggcgatgg
     3121 tctcaccgtg gccgcgcatg acgaagcgcg gcccctgccg gtgaatgagg cgcgggacat
     3181 agacgggata gtccgcgcta tgtttcaacc aacgctctcc cagcatgatc ttgcgggcca
     3241 actggccgac attgccgggc accagcgtcg ggatgcggcg acggatttcg tccaggagtt
     3301 ccggcgtgac aacttcgtcg gcatccagat gcaatgtcca ctcatgacgc aagccggctt
     3361 cagccagcgc ccaattgcgc tggtcggcaa aagaagcaaa tggatgcacc accactcgcg
     3421 cgccaagctc ggcagcaata cgttgactat catcggtgct gccatcgtcc accaccacca
     3481 ccacggcaca cccgcgcagc gcgcgcaaac aacggggcag gttggcggcc tcgttgtgcg
     3541 acagcacgac aaccgaaatg ggcaacggat cgttcatgcc actggtcaag ccgtcaccca
     3601 gccgccacgt ttcgttgccg caagaaacgc gcgggattgc caccgacaat ctggccgggg
     3661 ggcacatcgt gcaaggcgac cgcgcgcacg cccacgaccg catccacgcc gaccgtgata
     3721 ccggggtgga gataggcttc agcgcaaatc caagcccccg cgcccacgcg gatcggcgcg
     3781 gttttcaacg gcatattggg atggtccact tcgtgtgttg ccgcgcacaa aaaagcgcgc
     3841 tggctcaccg tggcgtgcgc gccgatttca atccgcgcca cgttatacac gtccacgccg
     3901 tggctaatgg tggcgtagtc gcccaccccg agattccacg gggcccagat ttgcgcagag
     3961 ggcatgatct gcaccccgcg gcccactttg gcgccaaata gccggagcaa aacgcgacgg
     4021 ggcgcatgcc agaaccatgg cgagggacgg aagaaaagca gacaggccaa ttgccaaccc
     4081 aaccgcgcca acttgttcgc ggctgaatgc ggactgggac acgcctcgcg atccacccga
     4141 atgcgagatg atggcacgtc agtcatgcgg ctcctttggc gtcaacgcct gctcgtaaca
     4201 tttcaacaat aagttcccga atgacgcaat gtcaaaaccc gcggcgcggg cgcggccgcg
     4261 ttcgatcatg gtttgccgca gggcggcatc cgacaataat tgccggcagg cgtcggccag
     4321 cgcatctgga tccgttgctg gggcgctgag cgcggcatca ccggccactt ccaacaaaga
     4381 tggcgcggtg gagcaaacaa ccggacaacc ggcggccatg gcttccaaaa tgggcattcc
     4441 gtagccctca tacaaactgg ggaacaagaa aagagcggca cggcgataga gatcgtgcaa
     4501 ttcggcatcg tccaccggcc ctacaaaatg aaccatcggc agcgtattgg ttctggcgac
     4561 cagctctggt gaaggcgacg ggccagcgag gaccaaatgc agatccgcgg aatcgcgcaa
     4621 ccgtaaaaat acgtccacca ccccgcggcg gtttttatag gaagcgttgt ggccaatgtg
     4681 caagatgaac cgtgcacccg gcggcaggcc cggcaacgca accggacgcg gcggcggcag
     4741 tggacgtaac ggcacaggca ccactttcac gcgcgccgtg atctttgcca gccgggcgag
     4801 atcggctttg gtggcggcgg aatcggcgca tagcagggca cattcacgca gggcggtgac
     4861 tccacgctgg atcaaccatc tggccggcca actgggccgg cgcggcaatt cgccgcgagc
     4921 ttgcagaagt ggtatcaaat catgaaccgt caccacgctc cgggcgcgaa gcgccggcgg
     4981 aataaacgcg gccatggagc catccagcca gtggtacaga tcagccggat gcgccgtcag
     5041 agcccggcgg gcatgacgca gccgccagac gtggtgccgc cacatggacg cgccgccgcg
     5101 cggatcaaag agatcgcagg tggcgaacgt ccaatccgcg gccagtggcg cgagcgaacg
     5161 ctgcacaagg tcggcatagg ccgccatgct gcctgcctga tcggggggcg cggttcgcac
     5221 ccaaaccaca cacttcatgg cgcaccttcc gtgcgcgggg catgctgcgc cgccaaccgg
     5281 gcaccggaca acaacaaccc ggcaataaag gctaacagaa atgcgaccag cggatcgccg
     5341 aagatttggg cggacactac ggcagaggcg acgttggcca ccaaaatggc gaagatgccc
     5401 gcggtcagat aaaacgagtc gcgctcccgc ccgaaacgca ccacatgata agcggtaatg
     5461 aacaggacaa cccccaccat cagatataac atcatgcccg gcacaccgaa ctccgcgaca
     5521 attttgctgg gcccgctttc ctgccacagg cgcggagttt cgacgttggg gatattgtgc
     5581 accccctgct gggacatgcc cagcccatag ccccagaagc cggattgcgc gatggtgccg
     5641 gccacggaga gcaagccgtg agtttcgaca cgctcgccca tttcggccag cgtgctgaca
     5701 tagaagcgtt cgagcgcctc gttgtgtacg accgaagcaa tcacatacca gcccaacccc
     5761 atgatcatcg cggccatccc cacgagggaa aaaatccggc gggcattttt gaaacggaaa
     5821 accagcagcg tataggtgcc gagaaagatg ggaatcatgg acagcatttt gcgccgcccg
     5881 cacagccaaa tgttgaagat tccccagatg gccaccgcaa cccacagcca acggaccacc
     5941 cctttactgc gcagggccaa taccgtagcc accatgaaca acaaagcggc atgccaaccc
     6001 atcacgtcgg gactacggaa aaacccggac agcatgtgga gcgcctcgcc ggtacggtga
     6061 gtcacccaaa tcgcgccaaa catatccgtg ccaacgaacg cttgcagata cggcaatttg
     6121 aacgcatcga gcggcccgcc catcaacatc aacgccgtaa ccgccgcgta gagcgtgaga
     6181 agtcgggcga tatcctcgga gcgatgtgga tagcgccacc cagcgacaaa gatcccaaag
     6241 atggtggaat agataaagat tcccaacagg gtaatcagcc aggaattatg gccataggtg
     6301 gcggaaatgg ccgcgggaat gaccaagtag atcgcgaagg tgcgaatcca caggccgagg
     6361 cggggaaatt ggctcaagaa gcggttgata ggcaattgac gggaaaacgc cgcagagcag
     6421 atgacaccca gccagatcgg catcccggcc aagaccagat aggccggagt gccgggaata
     6481 atcttgcgca aagggtcttg caacaagccc agcacaatgg cggcgccgat gccccagcgc
     6541 caattaacca gggcaaccag cgtcgccaaa gccaccaata gcatgaagac cattagcatc
     6601 gcgcaccccg cgcgcaggtt tgcactgcgg tcagaatacc cgcaatcacc tgctggtagc
     6661 tgaaagattg aatatggcgg ctcgcggctg tgccctgtcg cgccgccagc gcgggctccc
     6721 gcaagtagcg cgtcaggata ccggccaatg ctgcggtgtc gcccagctca aagacttctc
     6781 cggtttgacc gggcaagacc aaatcagccg cgcagcccac ggctcgggaa acaactgccg
     6841 ggcgaccgga ggccattgct tcattgacca ccaagcccca tgtttcgccg gcatccgacg
     6901 gcaacaccag cacatccgct gcggcataca cttccggcaa gcgcgattgg ttgataaatc
     6961 ccgcaaaggc ggtcgccacg cccaactccc gcgcgacccg ttcacattcc gcgcgcaatt
     7021 cgccatcgcc cgccacaaga acctgcgcgc gggcacggca atccgccggc aactggccaa
     7081 ccgcccgcag caagtccagc ggacgttttt tcggctggag tttgccgcag aacaaaaaca
     7141 cggttgcttg cggcgccacc cccaacgcgg cgcgaatctc tgcgcgacgt gcggttcgcg
     7201 ccgccgcttg cgccgcgaag aaatcgttgt ccaccacgta gggcgcccat aaaattcgtt
     7261 cttccgggca accatggaag cggtaaaact ctcgatttgc cgaaccaatc gccaaatatc
     7321 ccgcgtactg acgaagcagg aggcggtgca gaagatgctt ccatgctgcg cgaggtcgca
     7381 gcaaattagc ctcgccgcgc acgagacacg ggatgcccaa gcgccggcag gcccggagcg
     7441 cttgcaggca ggttttggcc acccacccat tcaccagcac agcgtccggc cgcaactctc
     7501 gcaggcggcg ggcaatgccc ggggtatcgc aaccgcggaa tgtggttact gatgggtgcc
     7561 gcgcacggtt ctccagcagc tcgctgcgat acccggtgcg caacggtaca tcccacgcaa
     7621 acgacgcacc aaaacccgcg ccctgttcaa cagggctcgg ttccatcgca taaagtaccg
     7681 tcacatccag ttccggcacc gccgccaatt tccggaataa cggcgccttg tactggatgg
     7741 gatgagtttc aacaatgacg aggcgatgcg gtgcacggcc ataccccatt gcttgcccgg
     7801 aagcgggggc ggtcatggcg cggtgtctcc cagcaaacgc ggaccctggc gatgcaaacg
     7861 caaagccaac accagcgcac gcagctcacc gatgaatttg acgggaatcc accacgggtg
     7921 ggccagatga aacttggtgc atacctcgcg gaaaggtcgg cgcagaagat accgcaagcg
     7981 tggccatccc cgtccgctgc ggagcgcaaa gtagtagtcc cccacgccat gaaccggcga
     8041 tgccgaggtc aggtggctgc cgctggcgcg ggtgccgccg cgcgccgcgc gcaagtggcg
     8101 gatcgaggcc gtcggctcaa accggatgcg ccccccggcg cggcatagcc gcgcgcaaaa
     8161 ctccgcgtca aagcgatagg aaaccggcgg cagaaagttt tcatcaaaac ccccgatggc
     8221 cagcgccgcc tcgcgacgaa aagacaaatt acccgacatc ccattgcgca cccaggtggc
     8281 agtggccgaa ctgaaatcaa agtgcagatc gccaaacggg tcggctgttg tcgcctggcg
     8341 gtaatttttc cgtggtgtct ggcccggctg caaaacttgc ccagccacgc ccaccaccga
     8401 gacatctgca tagcattgag catgcgcgcc gacaaaggac gggtccactt caatatcatc
     8461 atcgaggcag agaataattt cttgggtcgc ttctcgaatg ccgtgattca gggcggcggg
     8521 ctgggacggc tctgtgagcc gtacccaacg gatagcgcca gcggtgtgcc attgttgcaa
     8581 ctggcgattc gtcgctggct cgtgcgcgac ggtttggtcc agcacaagga tttctgccgc
     8641 gggaggctgc tgtgccagca actgtgccac cgtacgcaca agaacctcat cgcgaccata
     8701 ggtgggtatc gcgatgctaa acggaactgg cgaagtctgg gcgctcatct ttttttgtcg
     8761 ttttgggcca ccgcccagaa agtgcaggct ggcgggttga catccattgt ggccagaaat
     8821 tcatcaggat tttcaatccg gtgcacaggg tcatggcaag cataacgcac ggctattttg
     8881 ccccacgcgg aaagccggga ttcaacattt tcgtaaattt caacagaatc atattccgcc
     8941 cgcagccatt tcatcacgcc gcgtgtggta tccccgaaag cgcccaacat gtggggatga
     9001 atttctacaa agatcacggg atgccaacgg cgaaccagtt cctgcgcacc ggccagcgcg
     9061 cgaccttcgg cgccctccac atccattttg aggaaatcaa cgcgcgtgct gatgatgtca
     9121 tccagtggca cgagcggcac ctgatccaca gcggctgttg ccgccgtgat tcggccattg
     9181 attcccgaca taaagccgac ggttcctcgt tccgaaccgg ccgccacttc gtgcaactcg
     9241 acattcgaca atttgttttc ggcaatattt cgcttcaatt ccggcaaatt ttcccgggaa
     9301 ggctcaacgg caatcacctt gccctctgat cccaccaagc gtgccaacaa taaaaggtag
     9361 tagccgatat tggcgccaac atccacggcc gtgtccccct tggacaccag gcgcgacagc
     9421 aaaaaacgct cgccgataaa ccgctccccc tccagaaaca acagccgttg aaccgcggcc
     9481 cgtgatacat ccaccgccat actgaagcca aaaagctggc ggcgcaaaat acagggcgaa
     9541 ggtgcgccgg caaacgcacg ttccgcgcgc ggcagggttc ggaagcgata cacccatgac
     9601 acgagccgta gccatgacca gcgccacgcc atcggtcaag tcctctgccc aatgggtttg
     9661 gccggaacgc cccccactat ggagtatgga gccacatccc gattgaccac ggcccccgcg
     9721 gccaccaccg cgccttcgcc gatggtcacg ccgcaaagga tgacggcgtt ggcgccaatc
     9781 caaacatcat cgccgatggt cacgggggct tcctcccccc gctgctgaaa cataggcgcg
     9841 ccgcgtttca gcgaatggtt atgatccgtg ataaacaccc cgggcgcgat cagcgcccct
     9901 ttgccgatgg tcacccggag actcacttca atttccgtgc cacggccgat aaatgtatat
     9961 tcgccaatct gcagcgacgc attggggtcg ccgacattca accacacatt cggctgaagc
    10021 atgcaccggg cgccgaggtg aatggtccac ggatgctcca agcgcgtacc ccgtcccagc
    10081 agacatttgg ggtgaatatc cgcgccctgc cagcggtaga gataaacgcg cccgcgtgcc
    10141 agcgcaatgc ggataaattc ccgccaccga taaatccaac gcgcgatcat gatcctctcc
    10201 tgccggctga ttgtgacaac aattgggtat aaaacaattc ggtttccgcg gccacgctat
    10261 cccaagaata ggccgttgcc gtggccactg ctgctgccga gatgcaggcc gctgtggcag
    10321 ggttcgccca ccaatgccgt accgcagaaa caatgtcggc cggtgaatgg aagggcacca
    10381 acgcgccatt ttcacccggt cgaatcaaat cgcgcattcc gccggtcggg gtgccgacca
    10441 cacacaaccc gcgggccatc gcctccagaa ataccttgcc gaatccctca aacagcgagg
    10501 ggaacaaaaa tacgccatgt tcgtcatata tacgcacaag ctcctcttgc ggcatccaag
    10561 ggatagcctg tgcccgtggc cgcactgccg acggcagcgt ggccaaagcc gccgcgcaat
    10621 ctgcggccgg caaaacccaa gtgaacgtgg ccgtctcatc caactccagc agggtggagg
    10681 cggcttggct gaccgcggca caatctttgg cggcactata accaccgaca tggagcaacc
    10741 gattaagacg ggcgggattc caaggcgccg gaggcgcctc ttggaaaatc ggcgccggcg
    10801 cctgcgcgat acacgccact ttctccccgg gcgtgccata cgtggacata atataatcac
    10861 gatccaacgt actgctcacg atggtgcccg ccgcatggcg caatgcccag cgggcatgtt
    10921 gccccaaacg atgatccacc caccagccgg gaatgctgcg ccagccacgt cgtgtccggc
    10981 gacccgacgc acgaagccag cggcgaatta cttcttcagc gcggggttcc cagccatggc
    11041 tgcgctgcac gaccacgccc ggccgccgac gccggagatg ttcacgcgca gccaaccagc
    11101 aatgcgtttg attgatgtgg accacatcat acggctgccg gcggtacgcc gcggcgatgg
    11161 cagcttcata ggcacggggc agttcaaaaa tataatgaag attccagtgg cgaatccggt
    11221 gcggcaggtc atcgccccag atttcgtcaa tgtgatgccc ccggcggcgc aacgcggcca
    11281 gggtctgcca ttccgtgccg gccgcgcctg aatcgggatt gcgcacgagg tcggccgcaa
    11341 acaagatacg caaggcgacc gggctagcca tggcgttctc ctgccggcaa tgggcggttc
    11401 gccaaccgga cgctttgcca aagccgcaac caatgcttca cctgccacgg ctggcggcag
    11461 caggcttccc gcaaataggt cacggcccgc gggtcaccgt tgcgccgcaa ggtttcgcca
    11521 atgaaatacc atcccgccgc cttgcggcgg ctcgtcgttg tctggctgcc gcttggctgc
    11581 ggccggattt gcgcggctgc ctgcaagtag ggttcttcgc tggcgcctga acggcgcgct
    11641 tgaatcgtgc cgaaaaggca gtcgtgcaag cgccgctgtt tttttccttg gcgcatggaa
    11701 atgccatccg gaaaaatccg actgcggcac agtaccgcgg caatcccggc gaatgcgccg
    11761 tggtcggcga ggcgcaccca caagtcaccg tcttgtccgt aatagaattg ccaccggtag
    11821 ccgccggcag cgcggtaggc cgccgtccgg aacatgacgg atggatggga agaaggccca
    11881 ttcaccagat agccctcggc cgccgttgtc gtggcctgtg cgatccaatc gcccgcagtg
    11941 gcactgcttt gagttccgcg ttccacgaac agcggttccc aggcggggcc gcaaaattcg
    12001 gtccagcacg tcaccagtac aacttccgga tgcgcagcca aaaccgcctt ttggcgcgcc
    12061 agccgatctg gggtcatgac atcgcccgca tccatgcggg cgatgtaggt accccgcgcc
    12121 gccgcacacc ccgcgatcaa cgcgcgggtc agccccgcat tcgcctgggt caacacacgc
    12181 aaacgcgcat cccggcgggc gtactccgcg aggatttcac ccacgcgcgg atcggtggag
    12241 ccatcgttga cgactatcaa ctcgaagtcg cgttccgtct gcgccaatat gctgtccaag
    12301 gtgggcgcca acactgcggc ctcgttgaat acccccatca ccacggagat ggccggcgtg
    12361 ctcatgatgc cagcacctcc cgccaagcat tcacggcgcg gttgaattgc gcctgctgaa
    12421 tttcaccagc ggcataggct ggcggttggg taattgcggc aagggcgcgt gccgcgtcca
    12481 cggccgatgc tggctccagc agaattgcca actctcgttc attcggcggc aagcggtcat
    12541 gttgccgagg atgaaccagc gcgtagcaac ctgcggccag ggcgtcggtg accgtggttt
    12601 tgcagccact acccagcggg gatagcacgg ccaaggcttt cgacgcgccg aacaggggcc
    12661 acggctccgc cacgcgccca agcgccgtga tattgcccgg ccaatcatcc ggcggcgcat
    12721 cgagcagacc ggcgctggcc gccatgcccc gtccggccca agcgggctgt cgagccaacg
    12781 ccataaaccc ggcaacatgg ccccggccga tgggatcacg cgtgcccgcc aaacagagcg
    12841 catcggtttt ccgttgcgtc catggttgca aagtcacatc cgggcgcaac gccggccacg
    12901 gacaaaaata cggtgcggca accaacggag tcgtacccgc caaacgcgcc caataatgcc
    12961 gatcatccca cgggctgatg cccgccaaaa cgtcggcgac gcggcagcac cgccggtcac
    13021 ggctgaggag gcgcaagaac ccataaatat cgcgcggcaa accgcgcaac cgccaccatt
    13081 gtttttcggc gcgcgtccaa tgctgccggg cttcggcatt gacggtccgt agcacgattt
    13141 tcacctgtgg acagcaacgc cgcagttcct ccatggcttc cggccaaaag gtgtagtacc
    13201 agatgaccgt gtcattcgcg gagccgatcc gggcgatcaa ctcgctgatg ttgtcttcat
    13261 ggaaacgaaa ctcccgccac tcatttccga gacgggtcaa cacaaggcga ataccgtgca
    13321 aatccttcca ttggccagcg ccgggcaagc cttcccaggc cgtgggcaca accaaccaga
    13381 tcatgaccca cgctcccacc gttgccgggc ttgcgccaca cggtttgcgg tcggctccgc
    13441 catgatgcgc agcatgagtt catggttgcg gtgaccgctt gctttttgcg gcgcggggga
    13501 gtgccacagg tgaatcgccg gcagaaatga accgggatac aattttcggg tttgcaaacg
    13561 gtcaagaaat tcgaggtcct ccccgcccca ccccgcgaag cgctcatcgt gaccgccgag
    13621 ggccgcatag ctgtcgcgtc ggatcaccgt gaccatgccg ggcgtattct gttggacggc
    13681 ggcaacctgc cgcacaccgt ccacccggcc ggtgtggaca aactggtggc tggctggttc
    13741 atccaacagg aacaaaaacc gcagcgggcg gaccgcttcc cagccgcgga ccaaacaatc
    13801 catggcgcgc gccagaaacg tggggggcaa gacaccatcg gcatccagca gaaccaaggt
    13861 gcaatgccgg gctgcgcgcg cgccggcatt catcgctttg cttttattga aaggttcgtc
    13921 ggccacggcc ggcacgaaga cgtggcggat ttctgcaacg gcaaggtggg cataacgcgg
    13981 cgccgtgtca tgctcgcaga gcaagatttc agaatccgcc ccgatttgcc ggcgtaccgt
    14041 ttccatgacg aacgccacta ctggcgcgcg ctcgatcccg cgcaccggca ggattatgga
    14101 caacggtgca gtgggcggtt cgtggtcagt tgaccacaca tggcgcagca aacgtgcacc
    14161 cgtacgggga aatatccgac acaccgtcaa caaggaggag tcggaccaga ggcaaattcg
    14221 gccatggcta tcgggtgact tggccagccg ttcgtgacgg ttgcacagat ccagccattc
    14281 caagcccgcg cgccgagcca aatccggcgc ccgcaggacc aattccagtc gccagcgttc
    14341 atgccaccag caacccagct tctgcttgag agttggcgga ttcatggtgg gcggcccagc
    14401 aagcgacggc gcagccgggt cagcgcgaaa tagcccggca gcaagcgatg ttttggcaac
    14461 gcaggcgacc acagcagcga aggccgatag cacagcgcgc gccacgcggc gcgcgtcagc
    14521 tcagcatcgc tccaaggtcc cccgtagcga aacttgagca gggcggcgcc ggctgcggcg
    14581 gcgcggcgta acacccgcgg cgcggtgacc aattcgcgcg attcagccag catttgcaga
    14641 tattcatcat agattgcgac caagtgggtg tcgcgctgac ccggatgtgt gcggcacgct
    14701 gccaacgctg aacggagttg aacaacctcg gcatggaatg ccagccggta ccataaatcg
    14761 gtatcgggca catgggggat gcggggattc caaccgccaa gctgcaacgc caagtcccgg
    14821 cggaaaaatg ccgcggcttg atacacaaac acctcgcgcg ctagtagccg ggccagcgaa
    14881 tacgccggct gccgcggcga aaaaagaagt tggccggcca cgtccacgcg atccacattg
    14941 ccaaacacca gtccggccgc cggctgccgg gcgaacgtgg cggcggcatg ggaaaacgcg
    15001 ccgggcagat aataatcgtc ggaactttgg atggcgcaaa tctcgccgcg ggcgcgacgc
    15061 aggcctttgt tcacggcatc caccacgccg tcatcttttt cggaaaccca ctgcaactcg
    15121 ggccgctggc caaacgattg cagcacctgt accgtgttgt cggtggacgc gccatccatc
    15181 acgatgatct caaacgggcg ataatcctgc gcgaggcagg actcgatggt ttgccgaatg
    15241 aacgcgccct gattatagct gggcaccacc acggaaatca gcgggttggc tggcagcggg
    15301 ggcagtggtg gcgcggggga aggcatggcg gtgggtgtct tcacgcgggg gcggcgcaat
    15361 ccttgatcca cgcctgaacg agcggccatt cggcgggcgg gaaaatcttt tcgcggatga
    15421 gcgcgtcgta gcaggccaaa agattgtcgg ccaccgccgc ttcgcccgcg gccagatcga
    15481 ggccagtcag aatgtcggcg attcgcgtat tctgcaaata gccggggact tcgtcgtgga
    15541 aatcgcgcag gtagtcatgc gggttgcgtt cttgcaccac ctccggcgcg tggaacacca
    15601 cgccggcgcc gagcgcccaa aggcagcgct gagccacgaa gcttttccag atgtcgcaca
    15661 tccgaaatga acaatggctg ggcacataca atagcggata ggccgcgggc caccaccatg
    15721 tgctttgcgt attgaacggg caccattggc cggcggtcag cgccaatggc gcggccggat
    15781 caaaatcaaa ggggcgattt tgggttagcc gccacaccgc atccacatcg ggcgatccgt
    15841 tgaccagccc ctgttgcacg ggtgcgcgga acgaagcggc tgttgccgaa gcgggcgcgg
    15901 gatcagccgc caactgttcc aaggggaaac cgcgcggcca gatattccgg tcggcggaaa
    15961 aatagcgata ggcattgatc cagcccgcgc ccgccacggt gcgtggcgcg gctacggttt
    16021 ccgtgcgcgg cgcccaagcg ggcaacggcg cgttgtcgtc atcggtttca taaatgcacg
    16081 gtgcgccggc gcggatggcg tgcagatagc cgatattctt gcgtgcgtaa tggccggtgg
    16141 gcagtagctc ggccaaagca aaaccgctgg cctgctgggc agccaacgaa aggaaatctg
    16201 tgtgtggcag gtcgtaggca gcgggacctt ttttatcgcc ggcaattacg agacggccgc
    16261 cgacctcgcc cagccatcgg ttcaactccc gtaccgccgg ggtgggcggc tgaatggtgg
    16321 tgatgacggc gatgagagca ggcgccgtca tggggactcc gggccgggag cgatggcgca
    16381 cgcgccgcgc acggcaatca ggccttcgcg ggcttcgcca cgatagccaa aaccgtagat
    16441 atccgaatca atcacatcca gcggcaaggc gccatccacg cgatccagca gccgcacgcc
    16501 gggctcgaac acgaggacat ccacggaata ggcgccgctc atcagcggca catcggccac
    16561 ttcgaggcgc acaatctcgt ggggctggcg tttgacggcg cggaagccgc ccgccatcgc
    16621 ggaattgaag tggaatactt tttggtcaaa aaccgagcgc acggtcaggc cgaccactgc
    16681 ggcggggaac cggtccaagc cggccacgcg cacatccacc cgcagcgagt cgcccgtgcg
    16741 cacggaggcg gcgggctggc cggccgcgct gaccacgcgc gcggcgcaaa tcaagccggg
    16801 ctccggcggg cggggggtct tgcgttcggg gagcaacaca aagcccagcg cgtcgcgcgt
    16861 ctgctgttct tccgtcgaat gaatgtatgt ctggatgacc tcatcggcgg cgccgatggc
    16921 cgccacctgc ccctgccgca gcataagcgc gcgctggcaa agactgctga cggcggccat
    16981 gttgtgactg acgaagagca cggtgcgtcc accgccggcc acctctttca ttgcgcccag
    17041 acatttgcgc tggaaggtga catcgcccac cgcgagtact tcatcaatca gcaaaatggc
    17101 cgggcgcagg tgggccgcca cggcgaacgc caagcgcacc tgcattccgg agctataccg
    17161 cttgacgggc gtatccatga acgtttccag cccggcaaac tccacaatgc ggtcatattc
    17221 ctccacgatt tcgtggcggc gcaagcccag caaggtgcca ttgagaaaca cattttcgcg
    17281 gccagtcaat tcgggatgaa agccggtgcc gacctccagc agtgaggcga tgcgcccgcg
    17341 cagcgtggcc ttgcccgtgg tgggatcggt gattcccgcc agaatcttga gcagagtgct
    17401 tttgcccgcg ccattggcgc caatgatgcc aaagacttcg ccttgcggca cggtgaagct
    17461 gacctcgcgc agcgcctgca agattggcgc ggggggcgag cgatgaccgc gcagccggtc
    17521 ggctgcgcgc cgggtccaat cgcgatgaat ctcgcccagt tggaatgtct tgcccagttg
    17581 ggcaacttcg atgacaggcg tgctcatatc acatccgcca cagtgcgctc cacgcgccga
    17641 aaaaagatca tgccggtcag caaaatcagg aaggccaatc cgaagccagc gcccatgatc
    17701 ccccacccgc tgacgctctt gcccaacagc gcccagcgga agccctccac cacgccggtc
    17761 atgggattca actgcaacag ccactggtat gactccggca gcaggttcac ggcatagacc
    17821 accggggtga cgaacatccc gatttgcagc aggaacggca cgaggtgatg gatgtcgcga
    17881 tagcgcaccg cgatggcact caaccacagg gagagcccgc cggccaacgc gatggccggc
    17941 actaggaaaa gcgggaacag cgccatttgc gggccaaaca ccatgtccgc gcggtaccac
    18001 accatcatgc cgagcagcac gctcagcgag agcgccagat caatcaagct ggtaaacagg
    18061 taggacaacg gcaggatcat ccgcgggaaa tacaccttgc tgatcatgtt gctgccggcc
    18121 agcaggctgc cggtgccgct agtgatggtg gtggcaaagt aggtccaagg caacagaccc
    18181 gcatatagaa agatcggata cggtacgccg tcggacggca gattggcgag gcggccaaac
    18241 agcacgctga acaccagcat ctgcagcacc ggcttgagaa tgacccacgc aatgccgatg
    18301 gcgctttgct tatagcgcac ggtcaggttg cgccacatca gggtgaagag caaatcgcga
    18361 taagcccaca gctcgtccag tgccagatcg aaccagcccg tccgggggcg gcgcacatac
    18421 atgacctccc gcgcggccac gggttcaggc ctccggccgg gggttggggt ctgagcacgg
    18481 gcgcgcggcc acccacacgc cggcccacag cgcaatcagc agcgccagca gcgggatgcg
    18541 cagcggaaaa tccacgaggc aatgcaacgc accggccagc agcgcgagcc caaacgcgca
    18601 gcgttcgcag tcggcaaaag ccggcacctc cgccggatcg gtcgcgggcg gcgcacaccg
    18661 cgcggaaaaa gccagcgtcg tcagcgccag taaaatcgct gcgccggccc aaccgtattc
    18721 cacgagaaac tgcatcggct cgcagtgggc gtgcaggatg gtgtgtcggg catattcggc
    18781 ggcctgataa tacgggaaaa tgatggcgaa cgagccgggg ccgtagcccc agcgcggcga
    18841 ctccagccac gccgtgaacg agtggcgcag gatgccgctg cggtatgtcc attcgctggc
    18901 gatggcgctc acctgctgcc actcggccca gaacgcgcgc acggcgaacg cgaccgccgt
    18961 caaaaatgcg gccaccccca gcgccttgaa cacgggcgag agcggcacgc cggcaaaggg
    19021 atagcgccgc acggtgcgcc aatgcagcag gacgtgcgcc cccaccagca gcgccatgac
    19081 cgccaccccc gcgcgtgatt tactcatcac tacaccggcg gccgacaagg ccgccgccaa
    19141 ataaaacagg cccgccggac tggaaggcaa tcccgcctgc accgcgcgga agcgcgcgcg
    19201 cgccgcgccg gccaacacca ccggcagcaa cagattggca aaaaccgcgt aatgattttc
    19261 attcacgaag atgccggtaa attcacggat gcgcggcgca ttgggttcca aacgctggct
    19321 aatcaccacc aacgccattc ccgccgtggc cagtgccgcc aatgccggca gccaacttaa
    19381 ttgccgccgc gagagactga cgcccgccac aaacgcggtc cacaccgtgc cggcaaagag
    19441 tagcgcagcc cgcgcgccgg gcggataggc agccgccggc caaccggtgt gatagtcctg
    19501 cggcaccaat cccgcggtgg agctccaact gtgcgatgga ttccatacat aggcatacaa
    19561 cagcgccagc aggcccacgc ctgccgccca caggcgcagc ggcggcggct gcgtgatgac
    19621 gcacgccagc agcaacagca acgtcaacgc gggcaccagc gccgtgctcc acggggcggg
    19681 ctgcgccgcc ggccacgcca ctgccgtcag cagcagcacg agcaggggca ggagaacgat
    19741 ggatgccgcg cgtttcattc agcgacgggc gccggcggct ctgccggctg gctttggtac
    19801 tgctggtagg catagccgcc gtagcgccgg tagtagtaat acgtggcgag cgattgcaga
    19861 ttcacgccat tcatgatggc gcccaagcac gtggtgccgc cggactgaat ccggcgcagc
    19921 gcgtgcgagg cgccgaggct gtgcgtgacg ccatagcgca ccacaaacaa tacgccatcc
    19981 acgtgcggca acagcaccag ggcatcgcta atgcccagca ccggcggcgc gtccagcaaa
    20041 acgtgttgat acgtctggcg ggcttcgttc aaaatttcaa ccatgcgcgg attgccgaga
    20101 agttccgcgg ggtgcgtggg aatccggccg gacagcagca catccagatt gggcgccacc
    20161 tgcttaagca gcacatcggt ccagacgcaa tcgccgcgca gggcatcggc cagccccagc
    20221 gcgctgcggc tgctgcccac aatccgggcc agcagcgggc ggcgcaggtc ggcgccgatc
    20281 agcaacgtgc gccgcccatc ctgcgccagc gtgatggcca aattggtgga agtcaacgac
    20341 ttgccttcgc cagggccggc gctggtgaac atcaagacgg gcgcctgctt caattcatct
    20401 tggagcaaca aactggcgcg gagcgtgcgg aacgcttcgg ccacctcccc cgtttgtttg
    20461 aggtgcgcca ccatgccgcg ctcctccacg gttttgtcgc ggatgagtgg aatggtggcc
    20521 agcacgggca ggcccagcag ccgttcaacc tcctcattcc ggcggaagcg gtggtcggca
    20581 taatataaaa gaaaaatcgc gcccaccccg ccgatcaagc cgaacacaat cccgcggaag
    20641 atggcttgcc gcacctgcgg gcggaacggc gccttggccg ggcgcgcctc ctccgccagg
    20701 cgaatgatat ccaccggcag cgattccgag atgctggtct ccttcatccg ggcgatgacg
    20761 gcctgatgga tttcctgatc ggcctccacg ttgcgcttaa gctcgttata gcgaaccaat
    20821 ttgcggtcca gctcgaacga ttctttttct tgtgcggcca gcgccgcgcg caggttttgc
    20881 tcacgctgca cgagcgtctg ataattgttt ttcagcgcat agatggcatt gctgtaggcc
    20941 tgggtgaact ggccttgcag tttttgttcc agttccagcg cctgctgcaa ttccggatgc
    21001 tgctcgcggt agcgctgctg caacgccgcc accacgcgct gctgctcctg caatttctgg
    21061 gaaaccagca ccaggctctc atcgtgcagc acggtggcga ttttggccgg ctcgaccttt
    21121 ttatccatct ggctcttgac cgcgtgccac tgggtttccg cctgaatgcg ttcggtctgc
    21181 gccatggtca gcgcgctgtt gagcgccttg agtttttcga tgacaatatt ctggtcgttt
    21241 tcgagcgaga cggagccggt ctgttcgcga taggcctgca attcgagcaa ccccttttcc
    21301 agccgctcgc ggtattcctc ggagcgttcg cgcagccatt ccacggcttc gatggtggtt
    21361 tgcatccgcg aatccagatc ctgctggatg taggctttgg cgatggcgtt ggcgagatcg
    21421 ctcgcgatct ccggcacgga gtgctccacc ttgatgttga tcaatcggct gcgctcaatc
    21481 ggcgcaatct ccaccaaacc gctggcggca tccaccacgg cctgccgcgg cagattggag
    21541 aagaaaccgg gtcggtcgaa gaagccgccc tgctccaacg cggcttccaa taccttgcgg
    21601 ctgtgcagcg cggtgatgtg cgtgttgaag tattccaggc tacgggtgtt gatggccagc
    21661 acgtcctgat agttcaggat gcgcggcacc tgcgcctcca ccatcagcgt cgcggtgctg
    21721 cggtaatacg gcgtctggcg tttggcctga aaaataaaaa acagcaaaat gaccgcaaag
    21781 atcagcccgc ccagccaggc acgctcgacg aggatggcga cggcctcacg ccaatccggg
    21841 aagcgggaag tttcgttcgc gttgtccgcg acatcggcca gataaggata tttctgcatg
    21901 cggcgggcct atcttttaga acaacacttc cggcaccatg atgacatcgt tgggctccag
    21961 cggaatgtcc cgctgaccac gcttgccctt gagcagatcg gaaacgcgca ccgggatggt
    22021 cacttgctgg ccgtcgggac cgcgccgcac aatgcgcacg cggtcgggac tggccagctc
    22081 ggtgaagcca ccggcgccag cgaccgcttg cagcagggtg atggtttcgc cataggccag
    22141 cgggtagacg ccgggttttt tcacttcgcc cagcagcaca atctgggagc tttgcgtgga
    22201 aatcagtttg ataatcacgc gcgggttgac gagaaaatcc tcgttgagcc ggcgggtcaa
    22261 ttcaccttcc gcttcggtaa tcgttttgcc ggccaaggga attgtgccgg ccagcgagtg
    22321 acgaatctca ccggcggggg acagccggaa cgtccccgac aaatccggct cgcggtaaat
    22381 ctgcacatca atttcgtcgc ccggctggag cacatgctcc ggcaccgggg cggtggtggt
    22441 atctgccgcc ggcgcttccg tggctttggg ggtcttagcc accttggcag ctttgccgga
    22501 ttttttcggg gccggcgccg gcaacaccaa agcgcccggc ggcggcgatg tggtcggcgg
    22561 gctcttgcac cccgccactc cgctagccac caagccggcc aacagcagcc accggcatcc
    22621 gcgttgccat gttcgcgtct tcatgactcc gtttgtacct caaccgcggg cacttgaaaa
    22681 ggacggaacc ccgcccgttt agtagttcca actggcgcgc agcgtccagc gaccataatc
    22741 gtaggatgtg gcgtcctcgg cctcggcgcc cgcgccccgg ctgccatcgc gccgcatata
    22801 cgaataggcc aatcccacgg aaaagcgctg ccgggtccac caatccacgc ccacggaaaa
    22861 gccccaataa ttgtcggtgc ggccatcgcg cacccattcg ttttcatcgg cgtgcgggct
    22921 gccggaatac tccacgcggc cggcggtcag cgaagcattg ccgcgcagtc gctcgatgaa
    22981 caggcggcgc tccacgctca gggtgccgcc ttggtccacg gtgtccacgc catgcgcgtc
    23041 aatcgcggga cggatgtcgg catacagttc gccgcggaac gtggcgaggc cgaagggcga
    23101 actttcggcg gtcagcgcgg cgatccagtc gccggcatcg gcgcgttccg ggtcgtcgta
    23161 ggcgcgccac atatacccca ctcgcccggt ggtgtggaac ttgggcgact gcttgccgcg
    23221 cacgcccagc gagaggtcat aaaaatccgc gccgttgcgc gcatccgagt cgcgcagcgg
    23281 gtcatcacgc ccgatgccgc cctgcgcgaa cacctcgctc ttggcggtgg cgcggtgata
    23341 cagccggccc gcgccgtagt agcggtcata atccagatag cggtcggcct cgaagtcgtg
    23401 ccgctccgcg cggcccacca acccgaggct ggacttgccg gaaatgcgat gctccagcga
    23461 caagtcggcc agatagtcct gcttggcgag acgcgcgccg gccaccacgt cgaggtcttc
    23521 ctgccggtgg tagcccacct gcccctgtac ctggctcttg ccggtgcgat agaccacgcc
    23581 cagcttgagc atatgcgccg ggttgtcgtc cacatccggc gcgctgtcat acaccgggat
    23641 gctcacgccg taatccacat acacgtggtt gtccgccggg cggccccaga ggcccaacac
    23701 gccgggcaca aggcgaacgc tcgtgccctc ggttttctcg ccgggatcga gaaaaacatt
    23761 gtcctcgtgc gtgacggtca gcgtcacgcg cggcagcagc gcgccggtcg gcgccggaat
    23821 ataaaacgga tcgccgacgg ggctgccctg atccatatac gccgccattt cgaccccgcg
    23881 agtataaaac ccgttggcca aggcatcgcc cgccgcccgc gccagcgggg cgaatcccgc
    23941 ggcggccagc aacagactgg aaactagccg gagtctgatc actcaggcgt ctccacaaac
    24001 tggaagccct gaatcaactc gacctcgcgg cggtcgtagg gagttccatc ttcccgcaaa
    24061 acattttgct gccacagatc gggatcgctc tccgagcgga agccggtctg ttgcacttcc
    24121 aactgcgtcc ggccggccac cagcccctgg ttgaaccagc ccacgtggcc catggcgaac
    24181 agcggcagaa tcttgtcgaa ttggttgccg ctttggcgca tcagccagcc ggagcaaatg
    24241 atgggccgct tgtattgctg cagcagcatc aggcgggcct cgacggcttc cttcgtgtcg
    24301 tagctctgga acgtgatcaa atcggagcgc tccagcttgc gcgcggccat ggcgctgccg
    24361 aactctttcc atgccgccac cgccagcggt tgcgttgcct ccacgctgcg cgcccagttg
    24421 aacgtctgat ccaacagcgg cagcgactcc tcccacaggc cgctgttgcc ggcagtgtta
    24481 tacaaatccc aatacagcac acgcccgtcg cgcttgaacc ggcccatcac atccttgacg
    24541 tatttttcga gcgcgggcca ctgctggcga tccgtcacca tggcgtgcgc cggactgggc
    24601 acccactggc tgttgtactc tcccggcacc ggctccggct gcgggccgga caccggcgcg
    24661 cgccccgcca catcgagatc atcgaacagc accggcacca cgcgcaggcc gtgcttggcg
    24721 gccagctcga gtagcttgtc cacgcgcttg aggaagccgt ccgggtccgc ctgccacacc
    24781 gcgaactgca attgcacacg gatactcgta taaccaacat cgtgcgccca ccccagttcc
    24841 tgatcaatcg tgtccggatc gaacgtggcc tcctgccaca tctccacgga attgacggcg
    24901 gtgcgtggaa tataattcac gcccttcacg acgcccagcc ggttgtacca atcccaaatg
    24961 cggcgctcgc cccagcgagc gcccggcgcg ttctcgcccg cgcgcggcag ccgagtcggc
    25021 tgcgcgcccg cgacggcggg gcgaatggtc gggctggcgt cggtgaccag cgcgccatcg
    25081 tccacatcct ggaacggcgc gttcaggcca agataattgc cgagcccttc gcggtcgaaa
    25141 tccagcgggt gctcaatgaa cggctcaagg tcgctgaaaa agacattgca caactcgaac
    25201 gattcgcgtg cgggatcatt cggcacgcca gtggtctgca actcggcgcg ctcaccggcc
    25261 agcgtcaggc gggcgttatt gcccgcgccc agcgtgacgg gctcgccgcc gcggggcgtc
    25321 aatgtgagct cgcccgcgtg ggtgaacacc agccactggc cggactcgcc ctgggccacc
    25381 acgaactcgc cgccgtgcgc ctcaatcacg cctgccggcg tgaccacgcg gatatccagc
    25441 gtgggcagcg gcgcgccggc atgcacgcgg atgcgccccc gctccagatt gaggtgaatg
    25501 cggcggataa tgtcctccgc ggtgcgcggc aggccatcgc ccgtatgccg cagttgctcg
    25561 atctgaaact ggctttgcgg cgccacgcac acaatcgcgc ccggcgacat caccatgcac
    25621 agccgcccgt ccttgccagt gatgacgcgg ctgttttcgt gcagtgcggc gccctcgaac
    25681 agttggcgca aatcggtgcg gccttcgtga acgggcgcgg tgaatacgcg cgcctcggcg
    25741 tggccagtca tggcggccac gatggcctcg ccgctggccg gcgcggcggg cgccgccgga
    25801 acgccccaca gccaacccag cgccgccgcc caccccgcga cgcggaaaac gagccttgtc
    25861 ttgcggcgcg cttgcgctac cttttgctgc accatcatgg cgttatcctc attccattcg
    25921 ccaaccatca ttgcggaact gctgcgccag caaacgcccg tgacgtttcg cgcgcgcggc
    25981 ccgagcatga aaccgaccat tcgcgacggc agccaactgc attgcctgcc cgcgcccgcg
    26041 cgcctgccgc gcggcagcat tgtgctctac acattctgcg gccggctctg cgcccaccgt
    26101 ctgctgcgcc acgaccgcca cacgcgccgc atgtacgtcg tgggcgatgc cgcgctgacc
    26161 ggcggccgct ggctgccggc ggaatccgtt ttgggcgtgg tggtcgccgc gcgcgacgaa
    26221 cgtcgcgaac gcccactggc cacgcgcgcc gcgcgctggc gcggcctgct gcgccacgtg
    26281 ctgcggcccg cccgccgctg ggtgcatcgc tggcggcagc ggcttcacgc gctcacctcc
    26341 gccaagcggc catcttgaag ctccgccagc gcggccaccg ccgccggctg cttcgtgaaa
    26401 tggaagcgcc gcaccggcac gctcccgagc atgttgtcca aaccatccaa cgcggcttgc
    26461 gcccagtcat ccaggaacca cggcacggcg gccacatcca acagcgcgag gcgcgccatg
    26521 ctgggcggca cttcttccag ccattccgta gcggcctgct gcagcaggca aacgcccgcc
    26581 agcggcacag tgtccgccac ggcgaagcgg cccgaaccat gccacggcgt acccgccacg
    26641 cgccacccgt ccgccgttgg atacaccagc acgcggtcat cgttcagaat ttgccagccc
    26701 gcctcgtggc acaaatttgc cagcgtggac ttgcccgcgc cgctacgccc ggccagcaca
    26761 aggccgcgcc cgtcgtgcac ggccacggcg gcatgcagca gcacgccgcc cgcccgcgcc
    26821 agcaggcccc acgtcaaaat ttgatccagc ggatattcca gcggcgcctc gcacacttcc
    26881 gcgccgcaag ccacggtcag ctcggcgcgg gtcaacgcgc ggtccacccg gcaatgccag
    26941 cgcgcgtggt tgcgcccggc aaacccggcg cacaaaatca attccgcgcc gctctcccat
    27001 gcggcccaat tcaagccgcc gcgccacagc ggttcactct ccggcggcgg cagcgcgccg
    27061 cggcggatgc gcacagtcaa cgcgtcaacg ggcgcggcgc ccgccggcgc cgggacataa
    27121 aacccggcgt aggcccagcg ggagtagcgc acgttcagct ccggcgcggc ttccagccgg
    27181 aagctcaccg ggccaatcca cagttgaccg acatccttca cgaaccagct tcccacgcgg
    27241 taatcggcgg ccccacgcaa ctgctatcgt tctcaaactt gcagccggtg gacaacgtct
    27301 cggccagcgc caaatccacg cgcttgattt cgggtttggt gtacggcctg cgttcctgca
    27361 ggtcggtcgc gaccggcaac ggttccggat tcggggtcat ggggcatctc ctgtttcggc
    27421 ttgctgcgct gccgcgtggc gcaagcgggt ggtggcgcgc gcgtaatccg attccacttc
    27481 gggcgcgccg gtttcgaggc ggttgaccgc cgggcaatgg gtgcacgcgc cgcgcgccgg
    27541 gcccaccagc gagccatccg gccgggtgcg gcggcattgg cgaatcgcgc cgaggtcctc
    27601 cgcccacacg cgggcgaatg accgcgcgga aggcgcgcag taaaagtcgg tggccagcag
    27661 gcacggcgaa accccgccaa atggatcggc atgaaacgag gtttgccccg cggtgcacgg
    27721 atagagccgg tcggattccg gcaactcagc ggtgcggttg attttttcgc gccagcgcga
    27781 gcggcgctcc ggcgtggcca aatcggcggc gacagcttcg gccggcggca cgcgcagcgt
    27841 cagcggctcc ggcgaaccat ccgccagccg cgggaaaatg gcggcatcat gccggaacgg
    27901 cacgccgcac tcgcgcgcct gcgcttccag cgcgtccagc tcgtggcggt tcagcgtcag
    27961 caaaacggat ttgagctcca cgcgcacgcc gcccgccagc agccgacgga tgccggccca
    28021 cgcccgcgcg tgcgagccgg gctggccggt cacgcggtcg tgcgtggccg ccgtcgcgcc
    28081 atacaaacta atctccacct tgcgcggcgg aaactcctgg aacaagctca cggtctccgg
    28141 cgtgacgagc gtgccgttgg tgaacaccgt caccaccagg cccagctcgc gcgcgtggcg
    28201 ataaatctcc gcgaaatccg gatgcagcag cggctcgccg cccgtcagcg tcatggtcag
    28261 gcagcccgcg gccacccatt cggccatggc ctccttgatg gcgggcgtgg acagctcctc
    28321 cgcctgccgc cggtgctgcg cgatctggtc gccgagatag caatgcacgc agcgcaaatt
    28381 gcagcgccgg gtcagctcga acagcacagc caccggcgtg cgcgtccgcg ccgcccgcgc
    28441 ccggaatgcg gccagccagt cttgcgtgaa atccgccgga cagctcatgt gtccgcctcc
    28501 gccagcaatt gcgccgcgac cagttgggcg gcaaactcct cggcatccgc ctgcgcatcc
    28561 gccggcgcca cggcgaactc cgccgccagc tcgtccgcgg cctgcgcggg cgtgagcccc
    28621 tgcgcgaggc gcgtccacaa aaattcgccc gtctcgttga tcgggaacac ggcgttgccc
    28681 tgcgcggcga ggccggacac cggcaccagc agccggtcgg cgccgatgcg ccgcaccacc
    28741 acgcgcccca cgggccgata aaccgtcccc gcgctcatgc cgccctcccc gccgcaaccc
    28801 ggcctcgcga tacttttcca cagtgtggaa atattttttc cacagtgtgg aaaaaatcat
    28861 caaacatttt ccattgcgtg gaaaagtcac cccaaaggtt ttccattgtg tggaaaaatc
    28921 catcaaactt tttccattgt gtggaaaaat cgcgcaaagt ttttccattg cgtggaaaaa
//
LOCUS       DAOPNP010000014        28954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2090_length_28954_cov_7.909789, whole genome shotgun sequence.
ACCESSION   DAOPNP010000014 DAOPNP010000000
VERSION     DAOPNP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28954
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2090_length_28954_cov_7.909789"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PKI64_02450"
     CDS             complement(<1..925)
                     /locus_tag="PKI64_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD99901.1"
                     /translation="MNLLIQRARLDNQTRDIFIRDGKFAQIEQSLDPAALGTPLQVLD
                     AAGLAILPSFANIHCHAAMVLLRGLGDGLPLQAWLNDVIWPIERRLTPDQIYWGTLFG
                     CLEMIRAGITAVFDMYWQTPQCLRAFYESGLRVNFSHPNIIPPGQDALAGLRRDLEEH
                     LALAKSYPNRVELSVGIHALYTTTPEMRAVCRDFADTNDLLVQAHLSETRQGHEECLQ
                     ATGKTPTEVFAAEGLLNDRFVGAHGVWLTDSDRTLLATHGAAIAHCPSSNTKLASGAF
                     NEPAARRANLRFGLGTDGAATNNRYDMFSEMR"
     gene            1354..2097
                     /locus_tag="PKI64_02455"
     CDS             1354..2097
                     /locus_tag="PKI64_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOD99902.1"
                     /translation="MKQSGQLAMLKKSGRSGGLLLAAVALWAMLVLPAQAVLVVVDIG
                     WNYYGNSDIDNNGLMANFGLQEGSIVQVIAYNSGVGSAPGTDADDNFDIFGEYSGSLP
                     GEPNYPPNNQAPTDQTVYDPESVPPDHEIVYSTHIGPAIGGNANGYDWYNIYTSFFIS
                     GYDSIYIRVFGATDFPNGVVIASYWGISTVKTNDGSIGTWYVTYDDVTATNHVNYFEV
                     IPEPGTFALLAVGAGGLWLSKRRRKTGVA"
     gene            2213..3115
                     /locus_tag="PKI64_02460"
     CDS             2213..3115
                     /locus_tag="PKI64_02460"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD99903.1"
                     /translation="MKTSLRLTAMMALVWGIGWFPSSAPAQIISVSVGNDVPVKDQIG
                     RVLPGVNNFSNSSSLVEIRQTTPLGVIIAPDPDTGAGHPANPLMANSYIGKDAIFPGF
                     FSETFTNRLCPTNLYFARVFDNPQADKAIYYADTAPFEGTSNDVANIIVTFGAWKLRS
                     TGEPDIDSDGDGIPDEMENNITGTSPNDPDHDGDGFSDLFEVLFSDYLNPTEADARLE
                     LAIHAPAELSGDPHTVSWDTIAVPNMTYRLEFTDELDFENPYIEEIWSGTATETHLVV
                     DVEDWVTNSLHKGFFRVVVPYDVP"
     gene            3163..17844
                     /locus_tag="PKI64_02465"
     CDS             3163..17844
                     /locus_tag="PKI64_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOD99904.1"
                     /translation="MNAKNISLWTRMSRLALGVLVVAGSLLPASGQEQFSWKGDAPDG
                     YFKRGADGGRWYKHSNGDSYWDAYDDHGIIFFANDRFPNMTNNAGNPKITHQIYFSNN
                     TARTISGDGFRFYVYGGSSYPKIQNDSSGTHTFNSAITGDGDASDYMELNPVSGNLVF
                     NGAVNNNGSPIRVWGSNTLYINGVHSGGGQLQVKSAATVVLTNANTYSGGTWIEKGKV
                     EINTRSNALGTGAVNVGTNATLDIKHAMNLQPLALTLYNGTVTKTTTGSATWYGGLTT
                     YNSSTVSVSSGNLYFRGGFDIASGSTLVFSNVNAGGMDADTMTGAGTLVKYGGGTFRL
                     RPGTHSGNIELKQGNIWQYTGTMNSGGTLTMDGGTEYSADSTAARTLTKAVVIKGGVG
                     LAQNSTGGLEITNTVSLDNGTRIITNKNQVLISGKISSGGLGKAGDGTMILTGDNDYA
                     GGTVISGGTLQIGNNGTAGVVTGAITNNAALVWYRSDSVTNTGVISGTGTLTKQGAGT
                     LTLTGSSTMSGGTTVSAGTLLVSGALGSSAMTVNSGATLAGAGTVGPISSLAGTISPG
                     NSTGTAGTLTVNGAAALGGGTYTCDITGLGSTDCDKIAASGAVGASSALTINLPASAP
                     GGFNANSSYSWTIMSGSSASAANMTIGTKWAAAGTFGVSASGNTIVITYTAPAPAKPA
                     GLTASDGTSTAHVALSWTDGSGETGYVIWRHTANSFGSATAIYTNAANTTTYNDSSAN
                     PGQSYYYWVTATNASGSSAESDPDTGYRRLAAPTGVAATDGTSTSLITVTWDAVTGAT
                     GYHVFRDTDSDPAGATALGAKASGFTDAVTPGQIYYYWVVASNSTSSSTSDWSSADSG
                     YVKLTAPGDVAATENQSDKVTVSWSDVTGETGYTIYRHESNNSGAASIIGTAAANATS
                     YNDASATAGTTYYYWVRATNSTSQSMSDFSDPDTGMKTLTEPSKAASNILFSALANTS
                     YTVSWERGDGDYVLVVAHQGSAPTDPTDTTTYNANAAFGSGDTTAAGSYVVYKGTGTN
                     VSVTALSAETVYYFAVYEFNGAATPNYRTHDEPVANRTTLAAEPGTQASNIGISGPAE
                     VSMSGFTWTDGNGAGRILVAKAGSPVDAFPADGTAYTAHTNFGSGNQIGTGNYVVWVG
                     SGQPARISSLERDTVYHFRLFEYNGSAATINYNTNTAAGNPYSQTTMAANPSSAASDL
                     TVTSIGADTLTVTWTKGTAGTNTLIVIRSGADPAAPTDMVTYSANAAFGSGDTTAAGS
                     YVVYTNTGTSVTVTGLSPATQYRIWAYAFNGKDGSQNYRTSDPAKVNATTLMPEPSQA
                     TSISFDNLADTSYRVSFTAGNGLSRLVVAKAGGAVSWLPTDGTAYAGPNNNIGSAADL
                     GDGNRLVHVGTSPFTLSGLTAETVYHIRIFEYQGTNVTLNYNTNAASGNPGSRYTLSE
                     EPTAYGTLSATALSDTQIKLEWTAATGADGYVIVRKSGSAPSGAPADGSSYSQGNAIG
                     DGTVVHVATSGAAGSVTDNYSTAAGTTYYYLIFPYAYDGTPAHATYNYRTSGAVPGAN
                     ATTGPGEPASSSTLTSFLPVSGSSAKIIWDNAGSADGTIILVKADSAVSSNPTDWTNY
                     TASTTFGSGSQIGTGNYVVYAAAGKSGDVTVTGLSAGTTYHVAIYPYNGSGAMLNYRS
                     TSPATGSVVLLPDPSAASATVDGKTMVRLAWTKHASYDVMIVYKSGSASSAPTQGAGY
                     SVGSACGGGTVIYKGSGSTLEHVVASGTAHYYAFYSYSGGNYYSAGLAKSGATTAFAA
                     SEVVETFSYTNSTGIAGWNGEQGWDGAWYGADAGAFTNVSGSFSEQTNYPAPSGNKLW
                     VNPPDDTGKAVFRPLGQTYKEGRIYFGYILNYQYEGANKYAGLSLCWSNNEEKLFIGE
                     IYGQDKQLGIDNTGSAYTLSNGSGNDYIIVGYYDWSAGEAKAKAYKIGSQAVPTDEPA
                     SWDVTVSKASNTVGWVNTIRLAAGAGPSSGTPGNVYFDEVRIATNWAGLIGVVPSKPD
                     DPASASLTVDGHEMLRLAWAKNGASSDVMILHKTSAISTDPTDGTGYSVGNTIDGATV
                     IYKGSATALEHVVAAGSTNYYKFYSYNGANYYSDGLSKDQTMGSYAAHEVVNPFSYTN
                     STVLGAANKGGRGFGANAWSTGDSGTWTSAVNPASATDVAPRFFNMDNYPPMAGNLVR
                     LTGLSNGESGYADRQLDTPYENGTMYVAFLMSYRYDGANKWAGLSLFDNATEKAFFGK
                     GAGANYHTLAIGDGSSTWWASEDLRGTGANDGRGDTGRVYLVVGKYDFSAKRLQASVW
                     PQLANTFPATEPDTWQVSQDGVNISYINKIRLHAGSGDGGNTIDAAQFDEIRIGSSWA
                     HIVPTVCATWVGSNTLSGVAWTAAASEWLGNKLNFEFQSYPVGLGQVATLDFDWAQNG
                     SFATTNAMSWLKNENNNSYWSNRVQMTAAGVFTSRFTAAGSSCAAKTTNNPALTVQNL
                     NPPTGASATRDGTHTNSQINLEWTRGVSGVAKDTVIVRQTADASWTAPVNGTTYNAGD
                     PLGNGTVVYRGSATTFEDTGLAPDTTYYYRFYSENWSYYSVSYAAANASTAPGGQAIT
                     IDGNPSDWLGTPSAIYNNAASSVQQYIWTDKRTEERSDHADHANADIKEFRVFADATW
                     VYFLVRLTDITDASKPFIAVGVDTRRNDASTALTWLGDDSSTFIGSGYSNDGIAAAHY
                     PEYQLNVHHVDAASGARIEMWAHDSAYWYAPPTGGNTEVVISAANDAIEFRVARADLN
                     LTGTKTARFTVASFINTGAWNNDGDGTTHIADNTANAVDSITIPPWNMEDNDAKMSAW
                     LEDISDGDIDFWFDVKFGDSALVDNQRPTTPVLVAPTNNAGISASPTFQWQAASDNDG
                     QITGYLLEVSTNDQFNGISGTENGTVDLRIHLPATQTNYTFTTIASQYWWRVRARDNA
                     GALSVATTHTFRVVGKLDTEGPQPTLLYIGTNVAGYLAGAYDTHIAKYGPIQSVLDSE
                     LRDTNNVFGFVLRWDDPSGVYATNRNRDTGEFAFNIVSTDGRVSPNWDLLESNTVSGA
                     TTDWGIDQSFYASNTIAAGNTGQIVTNYVMAAFTITNYDPSIRYYLTVSAEDGCTDGG
                     SWVEYGSWNSFKAEDGGKFFSGWCADGPNTARNITTNYLIEILVTDDDIVPPSASLGL
                     GWKNNDTNAALVISNNVGRLDYVSGEGQDVLYQITDGALIGRPLAFSFNAYDSYYKGI
                     ALGTTATFVDKSRTLTNTSFVAAYWQTNWANYDAARSSVADTRSSNTMLTWYWPSITT
                     QDVTKLWGPDSLSGPLGVTNLIQLDLYDVDNDRVGDQASARVNFGRVVLVDDDATDPT
                     IVAASLQATGTGLAREYALTNLVEWTFDGTGAGRLTSTNVAAGVTSSPISLDSGTIQG
                     SDVITVQAGFAGGGSRSWLFNLTSAETSKTFKAESISFDSRVSSVNGPDTWEVWGKMP
                     GGGEVLWASGTIDLSDEENPVGTNWNSYSAGLAMPYAATNTVEFRIKAYVADTNHLTG
                     TSGTATWSIDNLVVSGYILGPAGGTQITDHDLATGNVSFRLQAADEYSGIDGTIGVTG
                     RAPRVDFWNTSSNVVPITNAFITNGWSADTNTTLTITGKSPAGVDKKKIVLGAGGGQL
                     TYYTRFTVTDADVDRDGDWRSVSSFITNVVYDNDTSRPARGYLFGGPLGVLVDGALTK
                     VVGSGNNREYRINDEQLQTMAATSLTMKVNLYDYSGWTVPVLSVSNSVAGLLSTNSWL
                     TGVHTADVDTTNKPDAAMEWQISQAQATTFFNDYENVTNEIRIVSVWDKDDDRQDASG
                     NNVDALELTNSRLGYMTFVDNDVGLPNVQSNWSVARTNWSIPKMYLGLPGDGTRSNLL
                     INGATVLANTNAGAVLANLTNRVYDSQLAKVTTAAPLSIVLPLFDTGGGGQGRTIKGV
                     QRGTTATESSNNGGAHDITNTSFSVGTVAVSNTANYRGDLSSPLAHTKIAAQFPTSVW
                     AFTSFSYAQVGNWLAAELSATNHGLSVAIYDADDNRPNDQRNRTVAVGTLQVLDNDTV
                     APSKPADVKVNGQEVAGPLSRDTAAWTNKPEFRVTFTPSVDGDPIGTDLEKTGIGEYR
                     TATQTSDIGPDSGTPMSVPAEGALADYGFELNGTGWTLAGGAAASTNYAVEGARSLRM
                     AIGATATQIISLANTNGLVPRITVIGAAYRGATDGTLSVIGQDANSNNVNGSSFVITN
                     TGVAGQWVIGSKAATTFDTTVDRIKVVLASSSDTYWDDIYIKVELLDNGDPVDEVATI
                     FIATEQGLTTNYLFAVDRDNNRPGDRMASSKSDDAVIPSFGTAYDITPPTPVPMPANA
                     ASTETVDDPTTQFDLQWITAGVGPDDEADDNYPHARFSGIDVLSPWRSYKIYYGTFDP
                     LNVPEDDPGHGNGNAYIYTNFIATGAYTNWPSVTASNTISDPSAAGTNYLALTNLSQG
                     TIRLYDLDYDQDYAVIIVGLDKAGNEGPASVSSWATNNTIRFAVTQGVYRSFATAPSF
                     PGAGGTNTFGVWTGQDKRASVLYWLAAGPTNEYGQYERVNKDYDLIYYDAASFQENSN
                     WTWKLAGTVRTNWFVETNSQYLAHGQMRFFRASYMDRWRRTNIVSGLPQRPLASEEVY
                     AMHNIVLSEGRNFVALHGIPYTNTFAAVFGTDTNIWPAGPSVAAGSTKVEFFSAGTNA
                     IASEVYFFGSDANWYKSGNATPVTTNLQATNFFTRGFSITLPTNLTGRGYATTNALDW
                     DVSKTNPIPAMVWHPILQVPTNGFTHTIHCGQDPRGTNEIRVYNVLALNLPVSVGPAA
                     LNLPTNFTRGTANTGDHIYVLESTTKNPRTDAIYCDVSNVWRFVNNNDPVGGSHFKPN
                     DMIVIVSRNGGTNNTWTWTYHPTNFYRLPTRWMGQ"
     gene            17998..18097
                     /gene="ffs"
                     /locus_tag="PKI64_02470"
     ncRNA           17998..18097
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI64_02470"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            18151..19770
                     /gene="dnaX"
                     /locus_tag="PKI64_02475"
     CDS             18151..19770
                     /gene="dnaX"
                     /locus_tag="PKI64_02475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOD99905.1"
                     /translation="MSYEVLARKWRPQQFAQVVGQDHVVQTLLRAIQTGRVAHAYLFV
                     GPRGTGKTTTARLLAKALNCQRRGANADPCDQCDSCREIVAGNNLDVIEIDGASNTKV
                     DQARELRDNVRYAPARGPFKVYIIDEVHMLSTGAFNALLKTLEEPPPHVKFLLATTDV
                     HKVPITILSRCQRFDLRRISVPEIVGRLREIAQAEKWQVSEDALLAIARGAEGGLRDA
                     ESALDQLIAFKGETIEEADVLGVFGLVSWHALAQLATAILQGKMDDVLATIAELDNNG
                     RDLQRLVQDLLGHFRNLLVWKHAPRLGQSFDLTEAQLAELRQQADLADGEKLLRVVEI
                     LTAAANQVRFALSCRVVVEVALLKASRAASVVSIDELIAQLQSSSADGGAPAPSAVVP
                     PAPVPATPPAPPAVAKRADKDDTAHLKTRWRSLLGVIAENAQLAAPYLKEAEPLRFEG
                     DHLILGFDPEFSGHLKQVDNSRTRYAIEQAVGRELKRPVGVKFTLEERAPAAKRPAPT
                     TPPAARRNLMDDPLVTKTVEMFAGKVYDIRE"
     gene            19780..20097
                     /locus_tag="PKI64_02480"
     CDS             19780..20097
                     /locus_tag="PKI64_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOD99906.1"
                     /translation="MANFMKMMKQAADMQKNMAKAQEALAALELEHSAGGGAVRVTIT
                     GDGNITAIRLDPALVQGGDVEMLEDLLLTAVRGAQDQARETAATEMKKLTAGLNLPPG
                     FGL"
     gene            20107..20697
                     /gene="recR"
                     /locus_tag="PKI64_02485"
     CDS             20107..20697
                     /gene="recR"
                     /locus_tag="PKI64_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOD99907.1"
                     /translation="MDALHQLAAALAKLPGIGRRTAERLAVHLARNPTSVAHDVAAAL
                     DTARRELTACQQCGSVTPKTENPCRLCADARRDNTILCVVEDPADIALIERSGEYHGR
                     YFALMGKISPLRGEGLGDLRLPALLERAATMQEVLLALNSDVESDATASYLRHVLAQK
                     YPQLKITRLALGIPAGSAIAFSDPVTLGRAIRGRTS"
     gene            complement(20907..21746)
                     /locus_tag="PKI64_02490"
     CDS             complement(20907..21746)
                     /locus_tag="PKI64_02490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD99908.1"
                     /translation="MNTNQFIDFLDSAQMFDLTMRLSVHTPPWPSYMPLGLQYFKRLA
                     GAHMGQGANGQIMTTSNHVGTHMDGEIHFYPAGRSIGNVPLREWVGPGVVADISKEVS
                     DYSIYTPKMITDKVEVKKGDILIINTGYHRYSWDQKTGDEIRYFVMHPGPSPDFDDWC
                     TKMKIKWIGVDCGSADHPMNTIIRTWHPGRFQECDAKLKKLYGKSFDELYPIKQYYQA
                     MHLKLFPKGIVHAENLAGDIAKLGSTRCWIGCFPLRGIELESSMCRIVAWLPPKAKKA
                     RKK"
     gene            complement(21798..22283)
                     /locus_tag="PKI64_02495"
     CDS             complement(21798..22283)
                     /locus_tag="PKI64_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOD99909.1"
                     /translation="MSEYRKLEDLLVYQKLCQLHIEMAMLSQHWPVEEKFELGSQIRR
                     SSNSAAAQLAEKYDDRHIRNKIEGVNRSRGEAAETVHHLYMACLKGYETQATYESYRL
                     RYQECIRMLNGMERTLERQLPETARRWPTVKEQTDDYYPTGCIDLSSTEPRTLNPQSE
                     P"
     gene            complement(22382..23785)
                     /locus_tag="PKI64_02500"
     CDS             complement(22382..23785)
                     /locus_tag="PKI64_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOD99910.1"
                     /translation="MSIQTLFSSPLRVVNVGIESFKEACVQAGAEAVQVDWRPPVDVA
                     PDAESILAKRQARIEKANQKVLDIIQAGTPKLVGLDIARNVIPGMTDNTILHAGPPIT
                     WDRMCGPMRGGIMAGLVYEGRASTIEEAEALAASGKIKYAPCHEHGAVGPMAGIITPS
                     MPVMIIHNEKFGNRAFCTLNEGLGKVLRYGAFGAEVTKRLKWMENTLYPVLKAALAHT
                     GPIDLKNMIAQALHMGDEVHNRNRAGTSLFYRAIAPAIVTTCENKEDATAVLNFINGN
                     DHFFLNLSMPACKAVLDAARDVPGSSIAVVMCRNGTDFGVQLAGTGNLWFTAPAEVPD
                     ALYFAGFSKDDANPDIGDSAITETGGLGGFAIAAAPAIVQFVGGSAADALRYTHQMYE
                     ITAGESRMYQIPALDFRGTPTGIDVRKVVEKNIVPVIDTGVAHKQAGVGQVGAGIVNA
                     PMGPFVKGYEALAKMLK"
     gene            complement(23802..25340)
                     /gene="fdrA"
                     /locus_tag="PKI64_02505"
     CDS             complement(23802..25340)
                     /gene="fdrA"
                     /locus_tag="PKI64_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HOD99911.1"
                     /translation="MMKIVGSVKQGFYFDSVTLMRVGRELTGLPGVAEASVVMGTEAN
                     KSILKMSDLLLPEFKKAGDQDLLVAIRAKDAKSAEAALQKAAELLTNKKKPATAGATV
                     PAPRDMASAVAALAGANLALISVAGRYAAREARKALEQGLHVMLFSDNVSVEDEVSLK
                     KLAHKKGLLVMGPDCGTAILNGVPLAFANVVPRGPIGVVGASGTGMQEVTCIIANEGS
                     GITQAIGTGGRDVKDAVGGISFLDAMAALAECPHTKVILLVAKPPQPGVLKKVYSLIR
                     RIPKPVVALFLGEGDRPATLEEAALLAVAHANGQDPAQALAHLTRRDAVLAAEARQLV
                     ANRRKPQGRYLRGLFSGGTFCAEAQVVLDGILRNVTSNAPLGDSRKLKNSWHPEKNAV
                     IDLGEDEFTVGRAHPMIDFTLRNKMILEQAADPETAVILLDVVLGHGANPDPAAELAP
                     VIRQAAKKVFVVCGVTGTIGDPQNRARVVETLRAAGAHVQLTNAAACKLAGLLVAEMA
                     RKPR"
     gene            26098..28005
                     /locus_tag="PKI64_02510"
     CDS             26098..28005
                     /locus_tag="PKI64_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HOD99912.1"
                     /translation="MKKISRRRGSQQVLVVIFISVLVAGLPALSNGVELGTRQQTIMP
                     RMVPPATPPVAPSNVAAYLTSPYGQWDWGPGTNEGRQLTLMPAEYVAAPRAARLLSFF
                     AMADVHMTDKESPAQTPFLGWRANFGEAGIGQLNPSAYTPVILATPQRLDAAVRTINA
                     LHRRAAFDFGIVLGDMCNSGQYNELRWFVDVMDGKLITPSSGAHVGADSIGYQMPFQA
                     AGLDRAIPWYAVLGNHDQFWMGIGYPSAKIQNAMVGDTVMNISPNGPLIPPGAEGTGM
                     YVGVVDGSTPEGAVVKWGETNRFATPPTVAADPDRRMLSTSLDSPTNFVNAFFASTSQ
                     PVGHGFNAGVTGNLAACYSFVPRADVPLKVIVLDNTCKQNLPGQNAMFYGGGWVDAAR
                     LNWLTNELQAGQNAEQLMILACHIPIMPQTTAADTNQTSTFYDRTAESNLVATLHNYP
                     NLLLVMAGHRHVNVVTPFPSPDAAHPERGFWQVETASLRDFPQQLCAWEILRNRDNSI
                     SIVTTAVDPALETNSPAWDSRTYGIAAERIFGRVALTDTSSHTYNVELVKQLTPAMQE
                     KIAGVGLPPGHSMHISLTESGVAIDFLGTLQSADAPVGSNIWDDVPTASNSSHTVLTS
                     DSRKFYRAVEP"
     gene            complement(28041..28361)
                     /gene="sugE"
                     /locus_tag="PKI64_02515"
     CDS             complement(28041..28361)
                     /gene="sugE"
                     /locus_tag="PKI64_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HOD99913.1"
                     /translation="MAWVILAIAGLFETGWAVGLKYSAGFTRPWATVWTVVSMIVSLG
                     LLGIALKHLPVGSAYAVWVGIGAVGTVILGIFLFGESANPARLLSALLIIAGIIGLKL
                     TTPI"
     gene            28388..28621
                     /locus_tag="PKI64_02520"
     CDS             28388..28621
                     /locus_tag="PKI64_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99914.1"
                     /translation="MAARTTSSNPQSTPAIYWLPLKEGAAGDINASAATAAKARKRGH
                     DVGRRCTGGEIEKKAASRQDAKDAKEDQWQVAG"
BASE COUNT         6062 a         8675 c         8672 g         5545 t
ORIGIN      
        1 tgcgcatctc ggagaacatg tcgtagcggt tgttggtggc cgcgccatcg gtgccgaggc
       61 caaaacgcag gttggcacgg cgggcggcgg gttcattgaa agctccggaa gccagcttgg
      121 tgttggagga agggcaatgg gcaatggccg cgccgtgggt ggccaacagg gtccggtcgg
      181 agtctgtcaa ccatactccg tgcgcgccga caaagcggtc gttcaggaga ccttcggcgg
      241 caaaaacctc ggtgggggtt ttgccagtgg cttgcaggca ttcttcatgt ccttggcgcg
      301 tttccgacag atgggcctga accagcaggt cattggtgtc ggcaaagtcg cggcaaacgg
      361 cgcgcatttc aggagtggtg gtgtatagcg cgtgaatgcc gaccgataac tccacccggt
      421 tcggatatga cttggcgagc gcgagatgct cttcaaggtc acggcgcagg ccggccagcg
      481 cgtcttgacc gggcgggatg atattgggat gcgaaaagtt gacgcgcagc ccgctttcat
      541 aaaaagcccg taggcattgc ggggtttgcc aatacatgtc gaacacggcg gtgataccgg
      601 cgcggatcat ttccagacag ccaaacagcg taccccaata gatttggtcg ggagtcaacc
      661 ggcgttcgat tggccaaatc acatcgttga gccaggcttg gaggggcagg ccatcgccga
      721 ggccgcgcaa aagcaccatt gccgcgtggc aatgtatatt ggcaaacgac ggcaaaatgg
      781 cgaggccagc cgcatcgagc acctgcaacg gtgtgcccaa ggcggccggg tccagactct
      841 gctcaatctg tgcgaatttg ccatcgcgga tgaaaatatc ccgcgtttga ttatccaaac
      901 gagcgcgttg aatgagcaga ttcatggtgc aggctcctgt tgcgagatga atccctgaaa
      961 aatatgggaa tcccggccaa cagaccagaa aaaagtggtt gtgaacaaaa aaattctcga
     1021 tttttttaga gaataagcac aaaacctatg ataaatacgg aaagcccggc tgatattcct
     1081 tgaaaacaaa ggctaaataa tggctaaaac ataaatccta aaatatattc aattagcgat
     1141 ttgtttttct gtgtctttgt tgcgggtgtt cgttgactca atggctggca agaaaaatag
     1201 cacgagcaat ctttatacaa aaatgtcgaa aaacaagctt gcaacattcc ctggttgggc
     1261 gtagttttgc ttgtgtaaaa ggatgggtgt atttcgacaa tgcaaaagca ttggcgtgtg
     1321 cgttgtatca ccttacactc catgcgagaa gagatgaaac aaagtggtca gcttgcgatg
     1381 ctgaaaaagt cggggcggtc tggtggcctg ctgctggctg cagttgcatt gtgggcaatg
     1441 cttgttttgc cggcgcaggc tgttttggta gtggttgaca ttggctggaa ttactacgga
     1501 aattccgaca tagataacaa cggcctgatg gccaactttg gccttcaaga aggctcgatt
     1561 gttcaggtga tcgcctacaa ttcgggggtg gggtcggcgc ctggcacgga tgcggacgac
     1621 aactttgata tcttcggaga atatagcggt tctttgccgg gtgaaccgaa ttatccgccg
     1681 aataatcaag cgccaacgga tcagaccgtg tatgatcccg aatccgtgcc gcccgaccat
     1741 gaaatcgtgt attccacgca catcgggccg gcaatcggcg gcaatgccaa cggctatgac
     1801 tggtacaaca tctacacctc cttttttatt tcggggtatg acagtattta catccgtgtt
     1861 ttcggcgcga cagatttccc caatggcgtt gtgatcgcca gttattgggg gatcagcacg
     1921 gtcaagacca acgatggctc cattggcaca tggtatgtga cctatgacga tgttacggcg
     1981 accaatcacg tcaactattt tgaggtgatt ccggagccgg ggacgtttgc tttgttggct
     2041 gtgggagccg gggggctctg gttaagcaaa cggcggcgga aaaccggcgt ggcctagtgg
     2101 gggggggcgg caaccgcaaa tttgcggtgc attatttgcg tatagtggga aagcgttgta
     2161 cggtttgcca cgggctgatt ttttgaggct gatgaattaa ggacggcaaa aaatgaagac
     2221 aagcctgcga ttgacagcga tgatggctct ggtgtggggc atcggatggt ttccgagttc
     2281 agcgccggca caaatcatct cggtttcggt tggtaatgac gtaccggtga aggaccaaat
     2341 cggccgcgtg ttgcccgggg ttaacaactt ttccaatagc tcttccttgg tggagatccg
     2401 ccaaacgacg ccacttggcg tcatcattgc tccggatccc gatacggggg cggggcaccc
     2461 cgccaatccg ctgatggcca actcctacat tggtaaagat gccattttcc caggcttttt
     2521 ttcggagacg tttaccaacc ggctgtgccc gaccaatttg tatttcgcgc gggtttttga
     2581 caatccgcag gcggacaagg ctatttatta cgcagacaca gctccgtttg aaggcaccag
     2641 taacgacgtc gccaacatca tcgtgacatt tggcgcatgg aaactgcgca gcacaggcga
     2701 gccggatatt gattctgacg gcgatggcat tccagatgaa atggaaaaca acatcacggg
     2761 caccagccct aacgaccctg atcacgacgg cgatggattc agcgacctgt tcgaagtgct
     2821 ctttagcgat tacttgaatc cgacggaagc ggacgcgcgg ttggagttgg cgattcatgc
     2881 gccggcagaa ctgtccgggg atccccacac cgtgtcttgg gacacgatcg cggtgcccaa
     2941 catgacctat cggttggagt tcacggacga gcttgatttt gaaaacccct acatcgagga
     3001 aatttggagc ggtacggcca cggagaccca cttggtggtg gatgtggaag actgggtgac
     3061 aaacagcttg cacaagggat ttttccgagt ggtcgtgcct tatgatgtcc cgtgatgaag
     3121 catggcggtc aggaatcggt ttaacctgag ggtggtagtg caatgaacgc aaaaaatatt
     3181 tcactttgga cccggatgag ccgtctggcg ctgggcgtgc tggtggtagc cggtagcctg
     3241 ttgccggcga gcggccagga gcagtttagc tggaaaggtg acgcccctga cggatatttc
     3301 aaacggggag ctgatggcgg gcgttggtac aagcatagca acggggatag ttactgggat
     3361 gcctacgatg accatggcat cattttcttt gcgaacgatc gcttccccaa catgaccaac
     3421 aacgcgggca atcccaagat cacgcaccag atttattttt ccaacaacac ggcgcgcacg
     3481 attagcggtg atggcttccg gttctatgtg tacggcggct ccagctatcc gaaaattcaa
     3541 aatgactcat ccggcacaca cacgttcaac agtgccatca cgggggatgg agacgcgtcg
     3601 gattacatgg aactgaatcc ggtatcgggc aatctggtat tcaatggcgc ggtgaacaac
     3661 aacgggtcac cgatacgagt ttggggcagc aacacgctgt acattaacgg tgtgcactct
     3721 ggcggggggc aactgcaggt gaaatcggca gcgacggtgg tgctgacgaa tgccaacacg
     3781 tacagcggcg gcacgtggat tgagaagggc aaggtggaga ttaacacccg gagcaatgcg
     3841 ttggggaccg gggcggtgaa tgttggaacg aacgcgacgt tggatattaa gcacgcaatg
     3901 aatctgcagc cgcttgcctt gactctgtat aacgggacag tgaccaagac aacgaccgga
     3961 tcagcgacat ggtacggggg actgactact tataacagct cgacggtgtc ggtttcgtcc
     4021 gggaacctat atttccgcgg cggctttgat attgccagcg gtagtaccct tgtcttttcc
     4081 aatgtgaatg ccggcggcat ggatgccgac accatgacgg gggcggggac gctggtcaaa
     4141 tacggcggcg ggaccttccg cttgcgaccg ggaacccata gcggcaatat tgagctgaaa
     4201 caggggaaca tttggcaata tacgggtacc atgaacagcg ggggcacgct gacgatggac
     4261 ggcgggacgg aatacagcgc tgatagtact gccgctcgca cacttaccaa ggcagtggtg
     4321 atcaaaggtg gcgttgggct ggcacaaaat agtaccgggg ggttggaaat cacgaacacg
     4381 gtgagtttgg acaatggcac gcgaatcatc acgaacaaga accaagtgct gatttccggc
     4441 aaaatttcga gcggggggct ggggaaggcg ggggatggca cgatgatttt gacgggggac
     4501 aatgactatg ccggtggcac ggtgatcagt ggcgggacgt tgcagattgg caacaatggc
     4561 acagcggggg tcgtgaccgg cgccatcacg aataatgcgg cgttggtgtg gtatcgttcc
     4621 gacagcgtga cgaacacggg ggtgatcagc ggcacaggca cgctgaccaa gcaaggcgcg
     4681 ggcacattga cgctgacggg tagcagtacc atgagtggtg ggacgaccgt gagcgcgggt
     4741 acgctactgg tgagcggggc gctgggcagt tcggcgatga ccgtgaacag cggcgccacg
     4801 ctggcggggg cgggcactgt ggggccgatt tcatcgctgg caggcaccat cagtccgggc
     4861 aactcgacgg gcaccgcggg cacgctgacc gtgaacggcg cggcggcgct ggggggcggc
     4921 acctatacct gcgacatcac cgggctgggt agcacggatt gcgacaagat cgcggcttcc
     4981 ggggcggtgg gggcatcctc ggccctgacc attaatttgc cggccagtgc accgggcgga
     5041 ttcaacgcga acagctcgta ctcgtggacg atcatgagcg gtagctcggc cagtgcggcc
     5101 aacatgacca ttggcaccaa gtgggcggcg gcgggcacgt ttggcgtatc ggccagcggc
     5161 aacacaattg taatcacata caccgcgcca gcgcccgcca agccggccgg tctgacggct
     5221 tccgacggca ccagcacggc gcacgtggcg ttgagttgga ccgatggttc cggtgaaacc
     5281 ggttatgtga tttggcggca cacggccaac tctttcgggt cggccacggc catctacaca
     5341 aacgcggcga ataccacaac ctacaacgat agctcggcca atcccggtca gtcatactac
     5401 tactgggtga cggcgaccaa cgcatccggt tcgagtgcgg agagcgatcc ggacaccggc
     5461 taccggcggc tggccgcgcc cacgggggtg gcggcaacgg atggcacgtc aaccagcttg
     5521 atcacggtga cgtgggatgc ggtgacgggg gcgacgggtt accatgtgtt ccgcgatact
     5581 gattccgacc ctgccggcgc gacggcgctg ggcgcgaaag ccagtggctt cacggatgcg
     5641 gtgactccgg gccagattta ttactactgg gtggtggcat ccaacagcac cagcagtagc
     5701 accagcgatt ggagctcggc ggatagcggc tatgtgaaat tgacagcgcc cggcgacgtg
     5761 gccgccacgg aaaatcaatc ggacaaggtc acggtgtcct ggtcggacgt gaccggcgag
     5821 acgggatata caatttatcg ccatgaatcc aacaattccg gcgcggccag catcatcggt
     5881 acggcggcag ccaatgccac cagctacaac gatgcgtctg caacggcagg cacgacctat
     5941 tattattggg tgcgggcgac caacagcacc agccagagca tgagcgattt cagcgatccg
     6001 gataccggca tgaaaacgct gaccgagcca agcaaggcgg cctccaacat tctgttcagc
     6061 gcgctggcca atacgtccta cacggtgagt tgggagcgcg gcgatggcga ctatgtgctg
     6121 gtggtcgcgc accaaggcag cgcgccaacg gacccgacgg ataccacgac ctacaacgcc
     6181 aacgcggcat tcggttcggg tgacacgacg gctgcgggca gctatgtggt gtacaagggc
     6241 acgggcacca acgtgtccgt caccgcacta tccgcggaaa ccgtttacta cttcgcggtg
     6301 tacgagttca acggcgcggc cacaccgaac taccggacgc atgatgagcc ggtggccaac
     6361 cgcaccacgc tcgcggctga gccggggacg caggcctcca atatcggaat cagtgggccg
     6421 gcagaagtca gcatgagcgg gttcacgtgg acggacggca atggagccgg acggattctg
     6481 gtggccaagg ctggcagtcc ggtggatgcc ttcccggcgg atggcacggc ctataccgcc
     6541 cacaccaact tcggatcggg gaaccaaatc ggtacgggca actatgtggt atgggtgggt
     6601 tccggccagc cggcacgcat cagcagcttg gagcgcgaca cggtctatca cttccgccta
     6661 ttcgaatata acggcagcgc ggcaaccatc aactacaaca cgaacacggc ggcggggaat
     6721 ccgtacagcc agacgaccat ggcggcgaat ccgtcatcgg cggccagtga cctgacggtc
     6781 acctcgattg gtgcggacac gttgactgtg acctggacca aaggcacagc gggaacaaat
     6841 acgctgattg tgattcgcag cggggccgac cccgcggcac cgacagacat ggtcacctat
     6901 tcggccaacg ccgcattcgg ttcaggtgac acgacggcag cgggcagcta tgtggtgtac
     6961 accaacacgg gcaccagcgt gaccgtgacg ggtttgtcac cggcgacgca gtatcggatt
     7021 tgggcttatg ccttcaacgg gaaggacggc tcccagaatt atcgcacgag cgatccggcg
     7081 aaagtgaatg caaccacatt gatgcccgaa ccgtcacagg ccacaagcat cagtttcgat
     7141 aatctggccg acaccagcta ccgggtgtcg ttcaccgcgg ggaacgggtt gagccgcctc
     7201 gtggtggcca aggccggcgg tgctgtgtcg tggctcccga cagatggcac ggcgtacgcc
     7261 ggaccaaaca acaacattgg ttcggccgcg gatctgggcg atggcaacag gctcgtccac
     7321 gtgggtacga gcccgtttac gctttcgggg ctgacagcag agacggtgta tcacatccgg
     7381 attttcgagt accaaggcac gaacgtgacg ctgaactaca acacgaacgc ggcaagcggg
     7441 aatccgggga gccggtacac actttcggag gaacctacgg cgtatgggac attgtcggcg
     7501 acggcgttgt cggacacgca aatcaagttg gaatggacgg cggccacggg cgcggatggc
     7561 tacgtcattg tgcgcaagtc gggatcggcg ccctcgggcg cgccggcgga tggttcatcc
     7621 tattcgcagg ggaacgccat tggcgacggg acggtggtgc acgtggccac ctccggcgct
     7681 gcggggtcgg taacagacaa ctacagcacc gcggccggca cgacgtatta ctacctgatt
     7741 ttcccgtacg cctatgatgg cacaccggcc cacgcaacgt ataactaccg gacatcgggc
     7801 gccgttccgg gcgcgaatgc cacgacgggc cctggtgaac cggcgagcag ttccacgctg
     7861 acttcgttcc tgccggtgag cgggtccagc gccaagatca tctgggacaa cgcgggttcc
     7921 gccgatggca ctatcattct ggtcaaggct gatagcgcgg tcagctcgaa tccgaccgat
     7981 tggaccaact ataccgccag cacgaccttt ggtagcggaa gccagatcgg caccggcaac
     8041 tatgtggtct atgcggcggc cggtaaatcc ggcgacgtga cggtgacggg actttccgcg
     8101 ggcaccacgt atcatgtcgc catttatcct tataatggca gcggcgcgat gctgaattac
     8161 cgctccacat cgccggcgac ggggagcgtg gtgttgctgc cggatccgtc tgcagcctcg
     8221 gccacggtgg atggcaaaac gatggtgcgg ctggcgtgga ccaagcacgc aagctatgac
     8281 gtgatgattg tgtacaagtc gggcagcgcg tcgagcgcgc cgacgcaggg agcgggctat
     8341 agcgtgggga gcgcctgtgg cggcggcacg gtgatttaca agggctccgg ctcgacgctg
     8401 gagcatgtgg ttgcgtccgg caccgcgcat tattacgcgt tctactccta cagcggtggc
     8461 aactactatt cagcggggtt ggcgaagtcc ggcgcgacca ctgcgtttgc tgccagtgag
     8521 gtggtggaaa ccttctccta taccaacagc accggcatcg ccggctggaa tggtgaacag
     8581 ggttgggacg gagcatggta tggagctgat gccggagcgt ttaccaacgt gtcgggcagc
     8641 ttctcggaac aaacgaatta tccggcgccg agtggcaaca agctgtgggt gaaccctccg
     8701 gacgataccg gcaaagccgt attccgccca ctggggcaga cctacaagga aggtcgcatt
     8761 tattttggct atatcctcaa ttaccagtac gaaggcgcca acaaatacgc gggattgtct
     8821 ctgtgctgga gcaacaacga agaaaagttg tttatcggtg aaatctatgg tcaggataaa
     8881 caattgggca ttgataatac cggttcagcc tatacgttaa gtaatggatc ggggaatgat
     8941 tacatcatcg ttggctacta cgattggagc gccggcgaag ccaaggccaa ggcatacaaa
     9001 attggatcgc aagcagtgcc gacggatgag ccggcatcgt gggatgtaac ggtttccaaa
     9061 gccagcaata ccgtaggttg ggtgaatacc atccggctgg ccgcgggtgc gggtccgagc
     9121 tccggcacgc cgggcaatgt gtactttgat gaagtgcgca tcgccaccaa ctgggcgggc
     9181 ctgattggcg tggtgcccag caagccggat gatccggcct cggcgtcgct gacagtggac
     9241 ggccatgaaa tgctgcgctt ggcctgggcc aagaacggcg ctagcagcga cgtgatgatt
     9301 ctgcacaaga cgagcgccat ttcgaccgat cccaccgatg gcaccggcta tagcgtgggc
     9361 aatacgattg acggcgcgac ggtaatctac aagggcagcg cgaccgcact ggagcatgtg
     9421 gtggcagcgg gctccaccaa ctactacaag ttttattcct ataacggcgc caattactat
     9481 tccgacgggc taagcaagga tcagaccatg ggcagctatg cggcccacga ggtggtcaat
     9541 ccgttctcct acaccaactc cacggtgctg ggcgcggcca acaagggcgg acggggcttc
     9601 ggcgccaacg catggagcac gggcgattcg ggcacttgga ccagcgcggt caatccggcg
     9661 tcggcaacgg acgtagcgcc gcgtttcttc aacatggaca attatccgcc gatggcgggg
     9721 aatctggttc ggttgacggg gctgagtaac ggcgagagcg gctacgccga ccgccagttg
     9781 gacacgccct atgaaaacgg cacgatgtat gtggcgttcc tgatgtctta ccgctacgac
     9841 ggcgcgaaca agtgggcggg cttgagcctg tttgacaacg cgacggaaaa ggcattcttc
     9901 ggcaaggggg caggcgccaa ctaccacacc ctggccattg gcgatggatc gagcacgtgg
     9961 tgggccagcg aggacttgcg cgggaccggc gccaatgacg gccgcggtga caccggccga
    10021 gtgtatctgg tggtgggcaa atacgacttc agcgcaaagc ggctgcaagc gagcgtgtgg
    10081 ccccaactgg ccaacacgtt cccggctacc gagcccgata cctggcaagt cagccaggac
    10141 ggcgtgaaca tttcctatat caacaagatt cgcttgcacg caggcagtgg cgatggcggc
    10201 aacaccatcg acgcggcgca gtttgatgaa atccgcatcg gctccagttg ggcgcacatt
    10261 gtgccgacgg tctgcgccac gtgggtcggc agcaatacgc tgagcggcgt ggcatggacg
    10321 gccgccgcca gcgagtggct gggcaacaag ctgaactttg agttccaatc ctaccccgta
    10381 ggcctggggc aggtggccac tctcgacttc gactgggccc aaaacggcag ttttgccacc
    10441 acgaacgcga tgagttggtt gaagaatgaa aataacaaca gctattggtc caaccgggta
    10501 cagatgacgg cggcaggtgt cttcaccagc cggttcaccg ccgcgggcag ttcctgcgcg
    10561 gccaaaacca ccaacaaccc ggcgctgacg gtgcaaaatc tgaacccgcc gacgggcgct
    10621 tcggcgacgc gcgacggcac ccacaccaac agccaaatca atctcgaatg gacgcgcggc
    10681 gtgtccggcg tggccaagga tacggtcatt gtgcggcaaa ccgccgatgc cagttggacc
    10741 gcaccggtca acggcaccac ctataatgcc ggcgacccgc tgggcaacgg cacggtggtc
    10801 tatcgcggct ctgccaccac atttgaagac accggcctcg cgcccgacac gacgtattat
    10861 taccgattct attcggagaa ctggtcgtat tactcggtga gctatgccgc ggccaacgcc
    10921 agtacggccc cgggcggtca agccatcacg attgatggca accccagtga ctggctgggc
    10981 acgccctcgg caatctacaa caacgcggcg agttcggtgc agcaatatat ctggaccgac
    11041 aaacgcaccg aagaacgcag cgaccatgcc gaccacgcca atgcagacat caaggagttc
    11101 cgtgtgtttg cggatgccac gtgggtctac ttcctcgtgc ggctgacgga catcacggat
    11161 gccagcaaac cgttcattgc cgtgggcgtg gacacgcgcc ggaatgacgc cagcaccgcc
    11221 ctgacgtggc tgggcgatga ctccagcacc ttcatcggca gcggctattc caacgatggc
    11281 attgcggccg cgcattatcc ggaatatcaa ctcaacgttc accatgtgga cgcggccagc
    11341 ggtgcgcgca ttgagatgtg ggcgcatgac agcgcgtatt ggtatgcccc gcccaccggc
    11401 ggcaacacgg aggtggtcat cagtgcggcg aatgacgcca tcgaattccg cgtggcgcgc
    11461 gccgatctga acctgacggg caccaagacc gcgcgcttta cggtggcgtc gttcatcaac
    11521 actggcgcat ggaacaacga tggcgacggc accacgcaca ttgccgacaa taccgccaat
    11581 gcggtagact ccatcaccat tccgccatgg aacatggaag acaacgatgc caagatgtct
    11641 gcctggctgg aagacatcag cgatggcgat attgatttct ggtttgatgt gaaattcggg
    11701 gattccgcct tggtggataa ccagcggccc accacgccgg tgctggtggc gcccaccaac
    11761 aacgcgggca tctcggccag ccccaccttc caatggcagg cggcatcgga caacgacggt
    11821 caaatcaccg gctacctgct ggaagtcagc acgaacgatc agttcaacgg catttccggc
    11881 acggagaacg gcactgtgga tttacgtatt catctgccgg ccacgcagac gaattacaca
    11941 ttcaccacga ttgcgtcaca atactggtgg cgggtacgcg cccgcgacaa tgccggagcg
    12001 ctttccgtgg ccaccacgca cacattccgg gtggtgggca aactggatac cgaaggcccg
    12061 cagccgaccc tgctctacat tggcaccaat gtggccggct atctggcggg tgcctacgac
    12121 acccacattg ccaaatatgg cccgatccag agcgtcctcg atagtgaatt gcgcgacacc
    12181 aacaacgtgt tcggctttgt cctgcggtgg gacgatccca gtggcgtcta cgccaccaac
    12241 cgtaaccggg atacgggcga gtttgcgttc aatatcgtca gcacggatgg ccgcgtctct
    12301 cccaactggg acttgctgga atccaatacc gtcagcggcg cgacaaccga ttggggcatt
    12361 gaccaatcct tctatgcctc caatacgatt gccgcgggca ataccgggca gattgtcacc
    12421 aactacgtga tggcggcctt caccatcacc aattatgacc cgagcatccg ctactatctg
    12481 accgtcagcg cggaagacgg ctgcacggac ggcggcagtt gggtggaata tgggtcgtgg
    12541 aacagcttca aggcggagga cggcggcaaa ttcttcagcg gctggtgcgc ggacggtcca
    12601 aacaccgcgc gcaacatcac gaccaactat ctgattgaaa tcctagtcac cgatgacgac
    12661 atcgtgccgc cgtcggccag cttggggctg gggtggaaga acaacgacac caacgccgca
    12721 ctggtgattt cgaacaatgt gggccgtctt gattacgtca gtggcgaagg gcaggatgtg
    12781 ttgtatcaaa tcacggatgg cgcactgatc ggcaggccgc tggcgttctc gtttaatgcc
    12841 tatgattcct attacaaggg aatcgcgctg gggacgaccg ccacctttgt tgacaagagc
    12901 cgcacactga ccaacacatc gtttgtggcc gcctattggc agaccaactg ggccaactac
    12961 gatgctgcgc gcagctccgt ggccgatacc cgcagttcaa acaccatgct gacgtggtac
    13021 tggccgagca tcaccacgca ggacgtcacc aaactgtggg gtccggacag cctctccggc
    13081 ccgctgggcg tgaccaacct gattcagttg gatctctatg acgtggataa cgaccgcgtt
    13141 ggcgatcagg ccagcgcccg cgtgaacttc ggccgcgtgg tgctggtgga tgacgatgcc
    13201 accgacccga cgatcgtagc ggccagcctg caggcgaccg gcaccggtct cgcccgtgaa
    13261 tatgccttga ccaatttggt ggaatggaca tttgacggca ccggcgccgg acggctcact
    13321 tcgaccaatg ttgccgccgg cgtgacctcc agtcccatca gtctggatag cggtactatt
    13381 cagggttcag atgtcattac ggtgcaggcg gggtttgccg gtgggggatc acgttcgtgg
    13441 ttattcaacc tgacttcggc ggaaactagc aagacattca aggcggaaag catcagcttt
    13501 gatagccggg tgagcagcgt caatggaccg gacacttggg aagtttgggg caagatgccg
    13561 ggcggcggtg aggtgctgtg ggcctccgga accattgatc tctccgacga ggaaaacccc
    13621 gtgggaacca actggaacag ttattccgcc ggtttggcga tgccctacgc cgccacgaat
    13681 accgtggagt tccgcatcaa ggcctatgtg gccgatacca accacctgac gggaaccagc
    13741 ggtaccgcaa cgtggtccat tgataacctg gttgtttccg gctacatcct tggtccggcg
    13801 ggcggaactc aaatcacgga ccacgacttg gccacgggca acgtgtcttt ccgtctgcaa
    13861 gcggcggatg aatatagcgg cattgatggc accattggcg tcaccgggcg tgccccacgc
    13921 gtggacttct ggaacacgtc gtcgaacgtg gtgcccatca ccaacgcgtt catcaccaac
    13981 ggctggtctg ccgataccaa caccacgctg acgatcacag gcaagtcgcc ggccggggtg
    14041 gacaagaaga aaattgtctt gggcgcgggc ggcggccaat tgacctatta cacgcgcttc
    14101 accgtcaccg atgccgatgt ggaccgcgat ggcgactggc gctccgtcag cagtttcatc
    14161 acgaatgtcg tctatgacaa cgacaccagc cgtccggcgc gcggttacct attcggcggt
    14221 ccgctgggtg tgttggttga tggcgcgctg accaaggtgg tcggcagcgg taataatcgc
    14281 gaataccgca tcaatgatga acaattgcag accatggccg ccacctcgct gacgatgaag
    14341 gtgaatctct atgactacag tggctggacc gtgccggtac tatctgtctc gaactccgtg
    14401 gccggtctcc tcagcaccaa tagctggttg accggggtac acaccgccga tgtggatacc
    14461 accaataagc cggatgccgc catggagtgg cagatttcgc aggcgcaggc caccacgttc
    14521 ttcaatgact acgaaaacgt caccaacgaa attcgcattg tttccgtttg ggacaaggat
    14581 gacgaccggc aggacgccag cggcaacaac gtggacgccc tcgagttgac gaactcccgc
    14641 ctcggctaca tgacctttgt ggataatgat gttggcctgc ccaatgtgca gagcaactgg
    14701 agcgttgcgc gtaccaactg gagcattccg aagatgtatc tcggcctgcc cggcgatggt
    14761 acgcgtagca acttgctcat caacggggca acggttttgg ccaacaccaa cgctggcgcc
    14821 gtgctcgcca acctgaccaa ccgggtgtat gacagccaac tggccaaggt gacgacggcc
    14881 gccccattga gcattgtgct gccgctgttc gataccggcg gcgggggcca gggccgtacc
    14941 atcaagggtg tgcagcgcgg cacaaccgcc accgaatcct ccaataacgg cggcgcgcac
    15001 gatatcacca acacctcgtt cagtgtcggc acggtggccg tcagcaacac ggccaattat
    15061 cgcggagact tgagctcgcc gttggcgcat accaagatcg ccgctcaatt tccgaccagt
    15121 gtgtgggcgt ttacctcctt ctcctatgcg caggtgggca actggctggc ggcggaattg
    15181 agcgccacca accacggcct gtcggtcgcc atctatgacg ccgacgacaa ccgccccaac
    15241 gaccagcgca accggaccgt tgccgtgggt acgctgcaag tgctggacaa cgacactgtc
    15301 gcgcccagta agccagcgga tgtcaaggtc aatgggcaag aagtggccgg gccgctctcg
    15361 cgtgataccg cggcttggac caacaagccg gaattccgcg tcaccttcac gccgtccgtg
    15421 gatggcgatc ccatcggcac cgacttggag aagaccggga ttggcgaata ccgcacagcc
    15481 acccaaacca gcgacatcgg gccggatagc ggcacgccca tgtcggtgcc cgctgaaggc
    15541 gcactggcgg attatgggtt tgagctgaat gggaccggct ggaccttggc cggcggcgcg
    15601 gcggcttcga ccaactatgc ggtggaaggc gcccgctcgc tacggatggc catcggcgct
    15661 accgccacgc agatcatttc gctggccaat accaacgggc tggtgccgcg catcacggtg
    15721 ataggcgcgg cctaccgtgg cgccaccgat ggcacgctgt cggtgatcgg gcaggatgcc
    15781 aacagcaaca atgtcaacgg ttcctcgttt gtgatcacga ataccggcgt ggccgggcaa
    15841 tgggtgatcg gctccaaggc cgccaccacc tttgacacca ccgtggaccg catcaaggtg
    15901 gtcctcgctt ccagcagcga tacctactgg gacgacattt atatcaaggt cgaactgctt
    15961 gataacggag atccggttga tgaagtggct actatcttca tcgccaccga gcaaggcctg
    16021 accaccaact atctgttcgc ggtggatcgc gacaacaacc ggccgggcga ccgcatggcc
    16081 agcagcaagt cggatgatgc ggtcattccg tccttcggca cggcctacga cattacaccg
    16141 cccacgccgg tacccatgcc ggccaacgcg gcctcgaccg aaaccgtgga cgatccgacc
    16201 acccagttcg atctgcagtg gatcaccgcc ggcgtcggtc ccgatgatga ggcggatgac
    16261 aactacccgc atgcgcggtt ctccggcatt gacgtgctct cgccgtggcg cagctacaag
    16321 atttattacg gcacctttga tccgttgaat gttccggagg atgatccggg ccacggcaac
    16381 ggcaatgcct atatctacac gaacttcatc gcaaccggcg cctacaccaa ttggccctcc
    16441 gtcacagcgt ccaacaccat ctcggatccg tcggccgcgg gcaccaatta tctggcgctc
    16501 accaacctgt cgcagggcac cattcgcctc tacgacttgg attacgatca ggactacgcg
    16561 gtgatcattg tcggtctcga caaggccggc aacgaaggtc cggcttcggt ttccagttgg
    16621 gccacgaaca acacgattcg ctttgccgtg acgcaaggcg tgtaccggtc gttcgccacc
    16681 gcgccgtctt tccccggtgc tggcggcacc aacaccttcg gcgtgtggac tggccaggac
    16741 aaacgcgctt cagtgctcta ttggctggcc gccggcccaa ccaacgagta cggacaatac
    16801 gagcgggtca acaaggacta tgacctgatt tattacgatg ccgcgtcctt ccaggaaaac
    16861 tccaactgga cgtggaagct ggccggcacc gtgcgcacca actggttcgt ggaaaccaac
    16921 tctcagtatc tggcgcacgg ccaaatgcgc ttcttccgtg cgtcctacat ggaccgctgg
    16981 cgccgcacca acatcgtaag cggcctgccg cagcgcccgc tcgcgagcga agaagtgtat
    17041 gccatgcaca acatcgtcct ctccgagggc cgcaacttcg tggcgctgca tggcattccc
    17101 tacaccaaca cgtttgccgc agtcttcggc accgacacca atatctggcc cgccggcccc
    17161 tccgtggccg ccggttcgac caaggtggag ttcttctctg ccggcactaa cgcaatcgcc
    17221 agtgaggtgt acttcttcgg ttccgatgcc aactggtaca agtcaggcaa cgcaacgccg
    17281 gtgacgacca acctgcaagc caccaacttc ttcacgcgcg gtttctccat cacgttgccg
    17341 accaacttga ctggccgcgg atacgccacg accaatgccc tcgattggga tgtgtcgaag
    17401 accaacccga tcccagccat ggtctggcac ccgattctgc aagtgcccac caacggcttt
    17461 acgcacacca tccattgtgg acaggatcct cgtggcacca atgagatccg ggtgtataac
    17521 gtcctggcat tgaacctgcc ggtgtctgtg gggccggctg ctctcaacct gcctaccaac
    17581 ttcactcgtg ggacggcgaa tacgggcgac cacatctacg tcctcgagag caccaccaag
    17641 aatccgcgca ccgacgccat ctattgtgat gtttcaaacg tgtggcggtt cgtaaacaat
    17701 aacgacccag tgggtggcag ccacttcaag cccaacgaca tgatcgtgat tgtctcgcgc
    17761 aacggcggca ccaacaatac gtggacgtgg acctatcacc ccacgaactt ctaccgcctg
    17821 cccacgcgct ggatggggca ataacgcccc tcctgtcacc catccggcgg gggcggcgct
    17881 tgccgctgcc ccactggtgc gaatgattgc attcgtccgc cggcaaggct atggtgctgg
    17941 gtgttcgccg gtacaccccc ggcgggccgg acgggctctc cccgcgccgg ttgccatggg
    18001 ttgggcccat gcgacggatg aggtcaaaac cccgccaggg ccggaaggca gcaacggtat
    18061 ggccgttctt ccgggtgccg tggttttctg gctcatggcg accggctcgg cgagagcgcg
    18121 accgcggtaa ccttcacgga gaagtgcagc atgtcatacg aagttttggc gcgaaaatgg
    18181 cgcccgcagc agtttgccca agtggtgggg caggaccacg tggtgcaaac cctcctgcgc
    18241 gccatccaaa ccggacgcgt ggcccacgcc tatctgttcg tcggcccgcg cggtaccggc
    18301 aaaactacta cggcccgcct gctggccaag gcgctgaatt gccagcggcg cggcgccaat
    18361 gccgacccct gcgaccagtg tgactcctgc cgtgaaatcg tcgccggcaa caatcttgat
    18421 gtcatcgaaa ttgatggcgc ctccaacacc aaggtggatc aggcgcgcga gctgcgcgac
    18481 aacgtccgct acgcgcccgc acgaggcccc ttcaaggtgt acatcattga cgaagtccac
    18541 atgctgagca ccggcgcctt caacgccctg ctcaagacgc tggaagaacc accgccccac
    18601 gtgaagttcc tcctcgccac caccgacgtc cacaaagtgc cgattaccat cctttcccgc
    18661 tgccagcgct tcgatttgcg ccgcatcagc gttccagaaa ttgtcggccg cttgcgcgaa
    18721 atcgctcagg ccgaaaaatg gcaagtcagc gaggatgcgc tcctcgccat cgcccgcggc
    18781 gcggaaggcg gcctgcgcga tgccgaatcc gcgctggatc agctcatcgc cttcaagggc
    18841 gaaaccatcg aagaagccga tgtgctcggt gtgttcggcc tcgtttcgtg gcatgcgctc
    18901 gcgcagctcg ccacggccat cttgcaaggc aagatggatg acgtgcttgc caccatcgcc
    18961 gagttggata acaacgggcg cgatctgcaa cgcctcgtgc aggatttact cggccacttc
    19021 cgcaacctgc tggtctggaa gcacgcgccg cggttgggcc agtcgttcga cctcaccgag
    19081 gcccaactgg ctgaattgcg gcaacaggcc gacctcgccg atggcgaaaa attattgcgt
    19141 gtggtcgaaa tcctgaccgc ggccgccaat caggtgcgct ttgcgctctc ctgccgcgtg
    19201 gtggtggagg tggccctgct caaggccagc cgcgccgcct ccgtggtcag cattgatgag
    19261 ctgattgccc aactgcagtc tagttcggcg gatggtggtg caccggcgcc atccgccgtg
    19321 gttccacctg ctccggtgcc cgcaacccca ccggcgcccc ccgccgtcgc caagcgcgcg
    19381 gataaagatg acactgccca cctcaaaacc cgctggcgtt ccctgctggg cgtgatcgct
    19441 gaaaatgcgc agcttgccgc accctacctg aaggaagccg agccactgcg cttcgagggc
    19501 gaccacctga ttttgggatt cgacccggaa ttttccggcc atctcaagca ggtggataac
    19561 tcacggacgc gctacgccat tgagcaggct gtcggccgtg aactcaagcg ccccgtcggc
    19621 gtgaagttca cgcttgagga acgcgcccct gccgccaagc ggcccgcgcc gaccacgccc
    19681 cccgcggcgc ggcgcaacct gatggatgac ccgctggtga ccaaaaccgt cgaaatgttt
    19741 gccggcaaag tgtacgacat acgtgaatag gagaccctca tggccaattt tatgaagatg
    19801 atgaagcagg ctgcggacat gcagaaaaac atggccaaag cgcaggaggc gctcgccgcg
    19861 ttggaactgg aacatagcgc gggcggcgga gcggtgcgcg tcaccatcac tggcgatggc
    19921 aacatcaccg ccatccggct ggaccccgcc cttgtgcaag gcggcgatgt ggaaatgctg
    19981 gaagatttgc tcctgactgc ggtgcgtggt gcgcaggatc aagcgcgcga aaccgccgcc
    20041 accgaaatga aaaaactgac cgctggcctc aatttgccgc cgggctttgg cctctaacca
    20101 acgcacatgg atgctctcca ccagttggcc gccgccttgg ccaagctgcc cggcatcggg
    20161 cggcgcacgg ccgagcggct ggctgtgcac ctcgcgcgca atcccaccag cgtggctcat
    20221 gatgtcgccg ccgcgctgga caccgcgcgt cgcgagctaa ccgcctgcca gcaatgcggc
    20281 agcgtcacgc ccaaaacgga aaacccctgc cggttgtgcg ccgatgcccg ccgcgacaac
    20341 accatcctgt gcgttgtgga agacccagcg gatatcgcat tgatcgaacg cagcggcgaa
    20401 taccacggac gctacttcgc gctcatgggc aagatttctc ccctgcgcgg cgaaggactt
    20461 ggtgatttgc gactgcccgc cctgctcgaa cgagctgcaa ccatgcagga agtgctcctc
    20521 gcgctgaact ccgatgtgga aagcgatgcc accgcctcct atttacgcca cgtactagcg
    20581 caaaaatatc cgcagctcaa aatcacccga ctggcgctcg gtattccggc cggcagcgcg
    20641 attgctttct ccgaccccgt caccctgggc cgtgccatcc gcggccgcac ctcgtagcct
    20701 ccccccgcgc ggctgttggt tcgacggatt ttttgtagcc gtgctcgccg agcacgaggt
    20761 tcaagcgttt ttgggggtgg gggagaggtc agaattcagg gttcaggatt tggatatttg
    20821 gagaaacaac tgtcatcctg tccggactgc tgtttcgaca gccttctcct gaaccctgaa
    20881 ccccgaaccc tcgctctgcc ctctggctac ttcttccggg cctttttggc cttgggcggg
    20941 agccacgcca cgatgcggca catgctggat tccagctcaa tgccgcgcag cgggaagcag
    21001 ccgatccagc agcgggtgga gcccagtttg gcgatgtcgc ccgcgaggtt ttccgcgtgc
    21061 acaatgccct tggggaagag cttcaggtgc atggcctgat agtactgctt gatcggatac
    21121 agctcgtcga acgatttgcc atagagcttc ttcagcttgg catcgcattc ctgaaagcga
    21181 cccggatgcc acgtgcggat gatggtgttc atcggatggt ccgcgctgcc acagtccacg
    21241 ccaatccact tgattttcat cttggtgcac cagtcgtcga agtccgggct cgggccggga
    21301 tgcatcacga agtagcgaat ttcatcgccg gtcttttggt cccacgaata acggtgataa
    21361 ccggtattga taatcagaat gtcgcccttc ttcacctcga ccttgtcggt gatcatcttc
    21421 ggcgtgtaga tggaatagtc ggagacctcc ttggagatgt ccgccaccac gccggggccg
    21481 acccactcgc gcagcggcac gttgccgatg gagcgccccg cggggtagaa gtggatttcg
    21541 ccatccatgt gggtgcccac gtggttgctg gtggtcatga tctggccatt ggcgccttgg
    21601 cccatgtgcg cgccggccag ccgcttgaag tattgcaggc ccagcggcat gtaggacggc
    21661 cacggcggcg tgtgcacgct caggcgcatc gtcagatcga acatctgcgc actgtcgagg
    21721 aagtcaatga attggttggt attcatgggg ttgtcctttc gttgttcgtt ttttttaaag
    21781 ggttcagggt tcggggttca gggttcagat tgagggttca gggttcgggg ttcagttgag
    21841 gatagatcta tacagccggt tggatagtaa tcgtcggttt gttccttcac agttggccaa
    21901 cgtcgagcgg tttcaggaag ctgccgctcc aaagtacgtt ccatgccatt cagcatacgg
    21961 atgcattcct gatagcgcaa acgatatgat tcgtatgttg cctgggtttc atatcctttc
    22021 aaacaggcca tatagagatg gtgcacggtt tcagcggcct cgccgcggct gcggttgacg
    22081 ccctcgatct tgttgcggat gtgtctgtca tcatattttt ccgccaattg ggctgccgcg
    22141 ctgttcgaag aacgtcggat ttgggaaccc agctcgaatt tttcttcgac gggccaatgt
    22201 tgggagagca ttgccatctc gatatggagc tggcagagct tttggtaaac gagcaaatcc
    22261 tctagtttcc gatattcgct cattccgcct gaaccctgaa ccctgaaccc taaaactctt
    22321 gctcctgaac cctgaaccct gaaattcttg ttcctgaacc ctgaatcccg aaccctgaac
    22381 cctacttcaa catcttggcc aacgcctcgt agcccttgac gaacgggccc atcggcgcgt
    22441 tgacgattcc cgcgcccacc tggcccacgc cggcctgttt gtgcgccacg ccggtatcaa
    22501 tcaccggcac gatgtttttc tccaccacct tgcgcacgtc aattcccgtc ggcgtgccgc
    22561 gaaaatccag cgcgggaatc tggtacatcc ggctttcgcc ggcggtgatt tcatacatct
    22621 ggtgcgtgta gcgcaatgca tccgccgcgc tgccgccgac aaactgcaca atggccggcg
    22681 ccgcggcaat cgcaaaaccg cccagtccac cggtttccgt gatggcgcta tcgccgatgt
    22741 ccgggttggc gtcatccttg gagaacccgg caaagtagag cgcgtccggc acttcggcgg
    22801 gggcggtgaa ccagaggttg ccggttcccg ccagttgcac accaaaatcg gtgccgttgc
    22861 ggcacatgac caccgcgatg gaactgcccg gcacgtcgcg cgcggcatcc agcaccgcct
    22921 tgcatgccgg catggagagg ttcaggaaga aatggtcgtt gccattgatg aaattcaaca
    22981 ccgcggtggc atcttccttg ttttcgcagg tggtcacaat ggccggcgca atggcgcggt
    23041 agaacaggga agtccccgcg cggttgcggt tgtgcacctc atcgcccata tgcagggctt
    23101 gggcaatcat gtttttcagg tcaattggcc cggtgtgggc gagggcggcc ttcaacaccg
    23161 gataaagcgt attctccatc cacttgagcc gcttggtcac ctccgcgcca aatgcgccgt
    23221 agcgcagcac tttgccaagg ccctcgttca gcgtgcaaaa agcgcggttc ccaaactttt
    23281 cgttgtggat gatcatgacc ggcatggatg gcgtgatgat gcccgccatg gggcccactg
    23341 cgccgtgttc gtggcacggg gcatacttga tcttgccgga ggccgcgagc gcctcggctt
    23401 cttcgatggt ggacgcacgc ccctcataca cgaggcccgc catgattccg ccgcgcatcg
    23461 gcccgcacat tcggtcccac gtgatgggcg gcccggcgtg caggatggtg ttgtcggtca
    23521 tgccggggat gacgttgcgc gcaatgtcca gccccaccag cttgggcgtg ccagcctgaa
    23581 taatatccag caccttctga ttggcttttt caatgcgcgc ctgtcgcttg gccagaatag
    23641 actccgcatc cggcgcgaca tccaccggtg gccgccagtc tacctgaacg gcctcggcac
    23701 cggcctggac acaggcttcc ttaaaagact caatgcccac attgaccacg cgcaggggag
    23761 aagaaaacag tgtttgaatg ctcatggtga gttccctttc atcaacgggg tttacgggcc
    23821 atttcggcca ccagcaatcc ggccagtttg caagccgccg cgttggtcag ttgcacgtgc
    23881 gcgccggcgg cgcgcagcgt ctcgaccacc ctggcgcggt tctgcggatc tccgatggtg
    23941 ccggtgacgc cgcaaaccac aaaaaccttt ttggccgcct gccgaatcac cggcgccagt
    24001 tccgcggccg ggtccgggtt ggccccgtgg cccagcacca catccagcag aatcaccgcg
    24061 gtttcagggt ctgccgcctg ctccagaatc attttgttgc gcaacgtaaa atcaatcatg
    24121 ggatgggcgc ggcccaccgt gaactcgtct tcgccgaggt caatcaccgc attcttttcg
    24181 gggtgccagg aattcttgag cttgcgcgag tcacccagcg gcgcgttgga agtcacattg
    24241 cgcaaaatgc catccaacac cacctgggcc tcagcgcaga atgtgccgcc gctgaacagg
    24301 ccgcgtagat agcgcccctg cggtttgcgc cggttggcca ccagttggcg agcctcagcc
    24361 gccagcacgg catcgcgccg ggtcaggtgt gccagcgcct gcgcggggtc ctgcccattg
    24421 gcgtgagcca ccgccagcag tgcggcctct tccagtgtcg ccggccggtc gccttcgcca
    24481 aggaagagcg ccaccaccgg cttgggaatg cgccggatca acgaatatac ctttttgagc
    24541 acgcccggtt gcggcggctt ggccaccagc aaaatcacct tggtgtgcgg acactccgcc
    24601 aacgccgcca tggcatccaa aaacgaaatc ccgcccaccg cgtccttcac atcgcgcccg
    24661 ccggtgccga tggcctgcgt aatgcccgag ccctcgttgg caatgatgca cgtcacttcc
    24721 tgcatcccgg taccggaagc ccccaccacg ccgatgggac cgcgcggcac cacgttggca
    24781 aacgccagcg gcacgccatt caggatggcc gttccgcagt cggggcccat caccaacagc
    24841 cccttcttgt gcgccagttt tttcagcgac acttcgtctt ccacgctgac gttgtcggag
    24901 aacagcatga cgtgcagccc ctgttccagc gccttgcgcg cctcgcgcgc ggcgtagcgc
    24961 cccgccacgg aaatcagcgc cagattcgcg ccggccagcg cggccaccgc cgaggccatg
    25021 tcccgcggcg cgggcactgt cgcccccgcc gtcgccggct ttttcttatt cgtcagtaat
    25081 tccgccgcct tttgcagtgc ggcttccgcc gacttggcat ccttggcccg gatggccacc
    25141 agcaaatcct gatcccccgc cttcttgaac tccggcagca gcagatcgga catcttcaaa
    25201 atggatttat tggcctcggt ccccatcacc acggacgcct ccgccacacc cggcagcccg
    25261 gtcaattcgc ggcccacgcg catcagggtg acggaatcaa agtagaagcc ttgtttgaca
    25321 gaacccacaa ttttcatcat cgctcgtctc ctggtggggc ggggcaagaa ccaaccgccg
    25381 aacaattcac catagcgtat ttgccaaaaa tggcgcgaga aaaattgtga cccggcgcgg
    25441 ggccgcttag ccggagacat caacgcaagc gccgcaaccg ccgcaagaga acgcaaacgt
    25501 ggtcatgatg tcggccgccg ttgtcccggc ggcgggattg cggaaaaagc tgcctctcgc
    25561 caagacgcca agcacgcaaa gacagataac gctgtgcggt gttggctgtt tctgtagccg
    25621 gcctcgctga ggccgggcaa aaacagcccg ccggggatta caaccccggc ctacaaatgc
    25681 ggcatgcagt tttgatgtag gccgccgttc taccggcggc ggaatggcat cccctttggc
    25741 ggacgttatt gcgaggctgt gaatggccca gtttattaat ttataggacc gaccccttcg
    25801 tacgacccct tcgctggatt ttgccggcta gtcgtctcca tccagccgcc tctagtttca
    25861 ccccaaaaaa ttggggttgc gaggagactt gcttgattaa tcatttcctg acacaatgcc
    25921 ccaatgaaat tggagcagtg ggacacgcct tgtcacccgg ccgcggtgtg gcatcgttcc
    25981 atttgggggc ctggctagct ttgccaggag atacttcggc cacgtcgaag cttttgtccg
    26041 cacaatcgca catcggggat ttggggcttt ggatgcaaaa aatagaaggc aaaaaatatg
    26101 aagaagattt cccggcggcg tggctcccaa caggttctag tcgttatttt catcagtgtt
    26161 ttggtggccg gactgccggc gctcagcaac ggggttgagc tgggcacgcg ccagcaaacc
    26221 atcatgcccc gcatggtgcc tccggcaacg ccgccggtcg ctccatccaa cgtggcggcc
    26281 tacctgacct cgccctacgg ccagtgggat tggggcccgg gcaccaacga ggggcgacag
    26341 ttgacgctga tgcctgcgga atatgtggcc gcaccccggg ccgcgcggct gctatcgttc
    26401 tttgcgatgg ccgacgttca catgaccgac aaggaatcgc cggcccagac cccgttcctg
    26461 ggttggcgcg cgaactttgg cgaagccggc atcggccaac tgaacccctc ggcctacacg
    26521 ccggtgattt tggccacccc ccagcgcctc gatgccgccg ttcgcaccat caatgcccta
    26581 caccggcgcg ccgccttcga tttcggcatt gtgctcgggg atatgtgcaa ctccgggcaa
    26641 tataatgaac ttcgctggtt tgttgacgta atggacggaa aactcatcac cccgagttca
    26701 ggcgcccacg ttggcgccga tagtatcggc tatcagatgc cgttccaagc cgccgggctc
    26761 gatcgcgcca ttccctggta cgcagtattg ggcaatcatg atcagttttg gatgggcatt
    26821 ggctatccca gcgccaaaat ccagaatgcc atggtggggg acaccgtaat gaacatctcg
    26881 cccaacgggc cgctgattcc gccgggcgcg gagggcaccg gaatgtatgt gggggtggtg
    26941 gatggcagca cccccgaggg cgccgtggtg aagtggggcg aaacaaaccg gttcgccacg
    27001 cccccaaccg tcgcggcgga tccggatcgc cgcatgcttt caacctcgct cgattcgccc
    27061 acgaatttcg tgaatgcgtt ttttgccagc acctcgcaac cggtgggcca cggatttaat
    27121 gccggcgtca ccggcaacct cgccgcatgc tattcgttcg tgccccgcgc cgatgtgccg
    27181 ctcaaggtga ttgtgctgga taacacctgc aaacagaatc tccccggcca gaacgccatg
    27241 ttctacggcg gcggctgggt ggatgccgcg cgcctgaact ggctgaccaa cgagttgcaa
    27301 gccgggcaaa atgccgaaca gctcatgatc ctcgcctgcc atattcccat tatgccgcaa
    27361 accaccgccg ccgacaccaa ccaaacctcg accttctatg accgcaccgc cgaaagcaat
    27421 ctggtggcca cgctgcataa ttatccgaac ctccttctcg tgatggcggg ccatcgccac
    27481 gtgaatgtgg tgaccccgtt tccctcgccg gatgccgctc atccggagcg cggcttttgg
    27541 caggtcgaaa ctgcttcgtt gcgagacttc ccgcagcagc tctgcgcatg ggagattctg
    27601 cgcaaccgcg ataacagcat ctccatcgtc accaccgccg ttgatccggc gctggaaacc
    27661 aattcgccgg cgtgggactc gcggacctat ggcatcgccg ccgagcgaat ctttggccga
    27721 gtcgccctca cggatacgtc gtcacacaca tataatgtgg aattggtcaa acaactgacc
    27781 ccagccatgc aggaaaaaat tgccggtgtg ggcctcccgc cgggacactc catgcacatc
    27841 agcctcacgg aatccggcgt ggccattgat ttcctcggga cgttgcagtc cgccgacgca
    27901 ccagtgggca gcaatatctg ggacgatgtt cccaccgcca gcaacagctc ccatactgtg
    27961 ttgacttcgg atagccgcaa attttatcgc gccgtcgagc cctagcgcgg ccggggaggc
    28021 tacggcctgc cgctcgtcgc ttagatgggc gtcgtcagtt tcaggccgat gatgccggcg
    28081 atgatcaaca acgcgctgag caagcgcgcg gggttggccg actccccgaa aaggaaaatg
    28141 cccagaatga ccgtgcccac ggcgccaatg cccacccaca cggcataggc gctgcccacc
    28201 ggcagatgct tcagcgcgat tcccaacagc ccaaggctca cgatcatgga aaccacggtc
    28261 cacaccgtcg cccatggccg ggtgaacccc gcgctatact tcaacccgac ggcccacccg
    28321 gtttcaaaaa gacctgcaat cgccaaaatc acccacgcca tgatgttctc caaatttccc
    28381 gcgcagtatg gcggcgcgca ccaccagcag caatccccaa tccaccccgg cgatttactg
    28441 gctgccgctt aaagaagggg cagccggaga catcaacgca agcgccgcaa ccgccgcaaa
    28501 agcgcgcaaa cgtggtcatg atgtcggccg ccgttgtacc ggcggcgaga ttgagaaaaa
    28561 agctgcctct cgccaagacg ccaaggacgc caaggaggac cagtggcagg tggcaggttg
    28621 aagctggaac agtcggagac atcaacgcaa tcgccgcaac cgccgcaaga aaacgcaaac
    28681 gtggtcatgc tgtcggccgc cgttgtaccg gcggcgggat tgaggaaaat gatgactctc
    28741 gccaagacgc caagcgcgcc aagacagatg atgctgtgcg gtgttggctg tttctgtagc
    28801 cggcctcgct gaggccggac aaaaacagcc cgccggggac ctcctcttgc gcaccttttc
    28861 cacactgtgg aaaatcctgc ccacgcggga tgaaataatt tccacggcgt ggaacttttt
    28921 gcgcaaagtt gttccattgt gtggaaaagt gggg
//
LOCUS       DAOPNP010000015        28119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2176_length_28119_cov_7.570304, whole genome shotgun sequence.
ACCESSION   DAOPNP010000015 DAOPNP010000000
VERSION     DAOPNP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28119
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2176_length_28119_cov_7.570304"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..945
                     /locus_tag="PKI64_02525"
     CDS             19..945
                     /locus_tag="PKI64_02525"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOD99915.1"
                     /translation="MSGVEKKWNQVAVLLGGPSSEREVSLRSGAAVAKALRARGYDAR
                     EVEVDKECRFVLPAGTEVAFVAMHGRFGEDGGVQAVLRAQGVPHTGSSPEACARAFDK
                     SQSKPIMVQAGIPTPPYELLRAGEPRTLPLPVVVKPVRQGSSVGVSRVFKEEEWPAAI
                     ALAHRLDEIALVEEFIDGRELTVGVVGDVALPVVEIIAPDGNFNYRAKYTLGVSKHVV
                     PAPLPAELAAACRDAALALFRALGCRGMARVDFRLRSDGRFYVLELNNIPGLTEISLL
                     PDAARAHGWSFEELCEIILNMVDCSATTEKGA"
     gene            953..1564
                     /locus_tag="PKI64_02530"
     CDS             953..1564
                     /locus_tag="PKI64_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD99916.1"
                     /translation="MNVLRVIANPKPVEQSASLKIEAAFMAALQQKHPDAQVTTINVF
                     QDDVPQLDALLLPAFFGAPPANAEIERRRDRQRELLAMFLAADLVVIASPVWNFNGPP
                     QLKAFIDAIMVAGKTFKYTANGPIGLVPEKKVVVCYASGMLYDHGQPPQQTLTPMLTT
                     QLGFMGITNVTLIGAQGQGAGREKAAATLAAAIEKAQAAAAQF"
     gene            2179..3072
                     /locus_tag="PKI64_02535"
     CDS             2179..3072
                     /locus_tag="PKI64_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOD99917.1"
                     /translation="MVRFTLAALKSKRRGNQSRESILRSKARSSARRQAVRRGVTTAV
                     VALLGVAAIGAALWWGGQAAWQILFTENEFFLIRDIEVTTDGALTMQEILDAAQVSKG
                     QNLFAVQPDTIREALLGKAMIASAQVGRRLPDTLLIEVGERVAVARLGRSDAGSPLAV
                     DLTGHVLGPSSVRASLPVILGVRDKGLKPGDVVQDPMLAEALQVLEICNLVAMRQELT
                     VATIEVGHPDRLTVGLTTGETVLLSRNKLREKLDRLRAMRIEARRRGLDLAVYDMTVD
                     RNFVGRPATGAPPAAAAAPAW"
     gene            3101..4297
                     /gene="ftsA"
                     /locus_tag="PKI64_02540"
     CDS             3101..4297
                     /gene="ftsA"
                     /locus_tag="PKI64_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD99918.1"
                     /translation="MKLEPIVALEIGTSRVRVFVGEPREDEGLIITAVGDCASSGMRK
                     GEVVDFNSLHPCVEAALRQAEEASNVSIHDVYLALSGSQIEAMPNHAEIQVDGEITAE
                     HTARVRELAKAVTLPADREILHSIIGQYTLDGLRGVTPEGMAGRTLEVNVLIVYGVHS
                     RMFNLVRVIQELKLSLAGTLFSGLCAAQAVLTPEQKMQGTLLIDLGGGTTDYVVYAQA
                     QIADAGVLAVGGDHVTNDIARAFQVTTADAEALKIKYADAMVSGADRTRWEPLPAAAG
                     GPPRQFKRMDMQSIVHLRMEETFGLIRERLKARTQLSFLSGGVVLTGGGAHLKNVTTL
                     AEKVFGLPCTIGVPRNVSGLTTNLALPEYATGIGAVLQGHRNALNAAQQAGGVWKKIG
                     RWFGVG"
     gene            4314..5507
                     /locus_tag="PKI64_02545"
     CDS             4314..5507
                     /locus_tag="PKI64_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD99919.1"
                     /translation="MTAQPENPQAQAPAPRCRILVVGVGGGACNVVSRALAGWNDAPL
                     VAGINTDAPALAQSDVALKLAIGKNVTKGMGTGGDVEVGRLAASEDLDALRNLVAGHD
                     LLLLVAGLGGGTATGAAPVLARVAREEGLLVLAYVTLPFAFEGARRQEQAQEGLVTLK
                     RNADAVVCLPNQRLHSLLPPDTSLPDAFAFVDRMMVAGIRGLWTLLAQNNMLNLDFSD
                     VQTLVENSGNECCFGYGEGAGAGKTEQALQSLLAGPMLDKGRVLANSGAMILGVIGGP
                     DLSLAELSTIHERFTAASRPGARISLGAAVLDDWPGRLALIVLAVEHWLPPPKPGALV
                     IEAGHTGPSAEPAGRRRAGRGQSELPLAPAPDRGRFQNVSPTLHDGADLDIPTYIRRN
                     IRIPT"
     gene            5595..6137
                     /locus_tag="PKI64_02550"
     CDS             5595..6137
                     /locus_tag="PKI64_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99920.1"
                     /translation="MKKMTWLSLLATLLAVSPLAFGAALVTKGANELGLEGDLDFATA
                     VGTRLEASAKYAYFFWDRISLGVQGVVFNNDAMHQFGLGVNAEYNFTLSSNYKPLFGT
                     DLVPFLGAAISYRHAKLFDEKESAVVFGGEGGVKFFLTDTTAITLSLVGELATEDIYD
                     DDLEATNKDLSLRLGMRFYY"
     gene            6266..6356
                     /locus_tag="PKI64_02555"
     tRNA            6266..6356
                     /locus_tag="PKI64_02555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6300..6302,aa:Ser,seq:cga)
     gene            6407..6781
                     /locus_tag="PKI64_02560"
     CDS             6407..6781
                     /locus_tag="PKI64_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99921.1"
                     /translation="MKNKIVIGGVVAFLCGLILNAHAQFGNAQPRVGDPNWTTDAHGR
                     VVPRIGNPGWTTDSHGRAVPRIGNREWTTDSRGNPVPRIGNPGWTTDSRGRAVPRVGN
                     PSWTTDSHGNPTPRIGTGYWDF"
     gene            6873..8168
                     /locus_tag="PKI64_02565"
     CDS             6873..8168
                     /locus_tag="PKI64_02565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD99922.1"
                     /translation="MVLGTASCHWPGGHTIAQEYPDAVASDPARLGWMVGAPPPPDRM
                     VRFDDGSYFRFPALRWSVANFRQLMPTVNVSRGLGAPVLLPQALRDDLDDVAFSPLGG
                     DGTMTWAESLPANYTDGIIVLHEGRVVYERYFGVLRPEGQHAAMSVTKTFVGTLAALL
                     VAEGQLDPERKVATYVPELQASAFGDATVRQVMDMTTGIRFSEDYADPKAEVWAHAVA
                     GNPLPKPADYTGPRSCYEFLQTVQPEGTHGEAFHYRTANTDALGWILARVSGQSVAQL
                     LETRIWRKLGAEQDAYMTVDATGTPFAGGGLNVGLRDLARFGEMIRNDGYYNGQQIVA
                     REAIADIRRGGNREAFAKVGYDLLQGWSYRSMWWVMHNEDGAFMARGVHGQALYINPR
                     AQVVIVRLASHPVAANAANDPITLPAFQAMARHLMQEKE"
     gene            8165..8776
                     /locus_tag="PKI64_02570"
     CDS             8165..8776
                     /locus_tag="PKI64_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOD99923.1"
                     /translation="MNLMLLSGKAWFWVVLVVLAGGTYALATRANLNFRHRPGDVLDR
                     LEGVAIYYNGGIHMTHGRNLAPDGYNLGIRYQCVEFVKSYLYERHGHRMPDAYGHAKD
                     FFDPRLADGQWNARRGMTQFANGSATKPATGDLLVMRPWLFNRYGHVAIVAAVTGNSV
                     EIAQQNPGPWGSTRAIYPLAIREGRWFIGQRRVLGWLRLPTAE"
     gene            complement(8968..9465)
                     /locus_tag="PKI64_02575"
     CDS             complement(8968..9465)
                     /locus_tag="PKI64_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99924.1"
                     /translation="MKKAPVAGWVLVTSRQPVYIPETTGDGIAEIIWKDVPAWKDPKT
                     GEIFLSGEARELLESTKARFLGILSPAQIRALRRSIKATQKGMAELLQLGEKTWTRWE
                     SGAERPSRSMNVLLCAIRDGRIGVDYLRSLVPPVVARHHPALAVPEPQIAEPAAKYRT
                     RSKRR"
     gene            complement(9462..9806)
                     /locus_tag="PKI64_02580"
     CDS             complement(9462..9806)
                     /locus_tag="PKI64_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99925.1"
                     /translation="MSKLLLIPAAWRNAVIAILESGDKNRIISTETADRDWNAAFPHA
                     FMFARHQAMAQALRADIHGRHIADMRPACDAYEFWFFFDQSRVLGKIGLLPNGKIIII
                     FSSHIPRKGDSL"
     gene            complement(9914..10456)
                     /locus_tag="PKI64_02585"
     CDS             complement(9914..10456)
                     /locus_tag="PKI64_02585"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOD99926.1"
                     /translation="MTLHRLPAIAIATLALVLAATASESLVVRGSGIRTKPILGALYE
                     LTLYVPESLQAADGPAILGADQPMAFHLTLKSSLITRKRFVETTTEGFAKAAASGYTS
                     PNTPAFLDQFANTEFKKGDTVIMRYADGALTTLYRTAGGTETPLATIPGLDLKQALFA
                     IWLGATPAQESLKTSLLATP"
     gene            complement(10486..13011)
                     /locus_tag="PKI64_02590"
     CDS             complement(10486..13011)
                     /locus_tag="PKI64_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD99927.1"
                     /translation="MNAPTFQLPAFALLRPLPKPAILWKDTAISYADLLTHSAALASL
                     YFSSPGERVVIFSENRPEWVSALLSIWRNRCVVVPVDAFSPAAELAYILGQTEPVAIF
                     CSAKTTPVVQEALGTLPALNARMVCFDNEDFPAIPSGCVADTPLMAGAEDTLAAILFT
                     SGTTGAPKGVMLTFGNIKVNLESVCDRVPIFTPQTRTFALLPAHHILPLVGCLLAPLY
                     SGGTIILAHSLDPADMVGTMRRHRATLLIGVPRLYALFRKAILDNLFKSPVGRLLYRL
                     SARINRLGVSRVLLYPVQKKFGGRLRQMVSGGAPLDRDVARDMTIMGFQVLEGYGMTE
                     CAPMITFPRPSAVRLGSCGQPCLPDSVRIEEGEVLVRGPHVFPGYWRNPAATAEAIQD
                     GWLHTGDLGYLDNENYLFITGRKKEIIVLPNGKKVNPAELEEKLLALSPDIKDVAVTL
                     QDDLLHALIQPVPGFLEGVPSQQAEHFRWQLLQPYNRLAPPAKKITQLTIVTADLPKT
                     RLGKLKRHELPPLATGTFSNGSAPAPKPEDLGPAYEAFDHFLRTELECPRVAPTAHWE
                     MDLALDSLARLSVLVFIEKTFGIKLPETAFQDYPTVLALARHADEQRLFFRQGGGGWD
                     ALLKAQPDEKPLELPRSRWIHPFLKNLFGTLLRLLFRVRAEGRQNIPAQGSCILVANH
                     QSYIDGLFVSMFLTNRFLRRTFYYAKRKHVKKGLLTWLARNSNVIVVDVGRDVQISLQ
                     MMAQALRSGGNLLIFPEGTRSPDGRLGDFKTTFAALAKEVQVPVIPVAINGAHQALAR
                     GTRLPRLGTRVTVRFLPPISPENFDTHQLTETTRTAIAQNLLI"
     gene            13272..>14677
                     /locus_tag="PKI64_02595"
     CDS             13272..>14677
                     /locus_tag="PKI64_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD99928.1"
                     /translation="MKWMKLLGVVGAMAVLSWTTAGANEEIASTWYGDQPDVYGLKSR
                     DTAGNNSYAYTPSPEQWRDKNIYQLFTDRFATDGVDRLAGYKPSWTCDGKPYPYNRNY
                     HHGGNWKGLQGQLDYLAGMGVTAVWISGVQQNDQGKDPNYTPYHQYHVENFFKCDPAM
                     GTFQDLKDLIDDCHARGMYVILDVAPNHMCDKNGINTGNENDDKQYWPNGHGSFGWWN
                     ESNKHALPFNNLNYFHPNGTINCWDCFPEQINGQFKGTDDLNTEHPDVQSILAKAFKN
                     LIDATDCDGFRVDAIKHVPYNWCRTWAQNIRDHAAWRGKNNFLMFGELFSYDNGALAS
                     WCADGYGFNSALHFPLMQAMKNVFGYGAGSDQLGWEMGAINQYGQGKENVIAFLDNHD
                     VDRFACTFGGGDAATAKKIMEPAMTFLYMAPPVPLIYYGTEHMFNQGGHPNGSNRTWD
                     NPDDGDWQRECMFDKGFQP"
     assembly_gap    14678..14777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14778..20705
                     /locus_tag="PKI64_02600"
     CDS             <14778..20705
                     /locus_tag="PKI64_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99929.1"
                     /translation="PTSFTSAGNGNIAFGSTKIPGKGSKIFIANFVDNSNDVFGGGGG
                     TPVGLWARNTYGYPTETATTADTIYINTEAGPASVTNKKVYLVYGFNNPSGAWPKVEM
                     ELNEEWDSNGGNWFHYDLGNLTTGRLDYVIAVETVEDGVTNTFWDNNGGGNYTMQIYP
                     APEPVNFSFHSVAANPVNPEPEGSVTVTVILRTAPGTNISALAARVGYDIFDPKDGPA
                     TNWPTYAMTRGNTITNDGTNVLSTFTYTVNNGLEAGYVMKYYIAASNGASILYANNGG
                     QDYSVVIKDWPAVPADIEISTSNHTTQASSATVAGTAGANIADGLYWTNSLTGDTAQF
                     ELTGHWSAAIPLNVGTNVITVYGYEAGTTTTNATDKASNYATWTHNSNQGSGFGNWSL
                     VGVEDYAGHFIAVGNETCNLTKPAFGMWAHSDNTSEVYRPFSQALGAGDTFSISIDNG
                     SIDSGHSTGIGLQSASGENLWEFYFVGGDFTYTMTGGATDIGWTSGGLTIEFTLTSAS
                     TYSAVITPLGGSARTYTGSFSGTIARFHAWHYSANGGEDHNFYFNDLKITSASLGTKK
                     QDSVTITREDDGRPRIEATGSRSISIGENGTAFTLGLSLTNATAASWSASLTALNGSH
                     VQSWSNVAGATWNWTWTAYAAGTWRLRVTAHAAGGAAITNTTLVLTATEGMSYTLGNA
                     WHCPTNNEPFEAKYMRHPVTPGVGETIEIYVGNYHPQGDMNGGSLHYRWGTSGAWSST
                     ALSWTNNTNGDDNRYWVGTQVVPGDKKGGVFQYYLEVTYTDGSPTNTFVAMDPDQSAM
                     YKTFKREAAAQATPFEVGIRDDAGKEPGYIWHGGDITVAGEDSVQIGAKIGYIKDGAK
                     WADTAVVRYRVAEDAKGAKQARGLKRILKSGRSPRSWNALTNQVSMAYEGEIPDSSPN
                     GNAMRWVATITDSNLLNERAVLIYEIYAKGSNGVWRQAEYNAGGGSSTFEYRPYSEGA
                     DDLQVRGNTRDYDSGWLAADYTTTKYFINEADAQDTVTITVRYKAPANAQKVELFTNL
                     GRRDFADADMNNDGWPDGIIPPAGNTITAENTYNVGAGYWQAIPMTAVGGAWQKTLTV
                     NKCGAYRITARFLAAGAAEGEWTWYSEGPDNSLRRDHAVVISPRKALEQTMYELNVFT
                     IKATNTAQSGRGTFEKLSEKLNAGSGAYDEFSLAYLNKLNINCLWFQPIHPSGGDRVE
                     NDPATSNRYWPGSPYGTKNYFSVNPDMSDSATESAAVAAFTNFVRLCDRARGETMPEN
                     ERTVNTVNVMLDGVMNHTSWDAIYGEGLALATNGLGAAAIAELTNAYGNIAALAPSER
                     IATTGKLGINWYSAKTNYAKPATYYNTAHDNDIGVAPERVDFGKWDDVAELLYGNYST
                     MVRWDDRVPPDYNLTEETSRMYNEDDRYYYNEMLPATKLLWKYMASYPEYWLKKTGHS
                     GTNQPGQKDGNGVLLDDYGIDGLRCDYAQGLPSQFWEYLINRTRSKKWNFLFMAESLD
                     GGLVGKRSNRHFDILNENMVFRFTQDKVSEPAPFQAALEDRRTLYGNGIILLNLSSHD
                     EPLPFGDPWATASRYAMVSAIDGVPMILLGQEHAISPYGGPDGDTIDKWKGFYFFEQN
                     FGKWIPHFKKWNQMYVWDDPCYPSGDFRESRKMAELYGRINKARLMSPALRSKNRWFL
                     NDNPRMMTVAKWETPGANPNVRDAVIASVLFMNEGDGGLVGNAQVYDLSPFADKLGLE
                     NRADRLYNIRNLAASDPAALIWPEAKAGSEIITNFYIGLDGGMDGTNANPAKVWANGA
                     VVQYLQFVDVTAYAPPVIQVGTIPTNKTVGQSVSISVTATGDGEPIVTLTNAPAGLLN
                     SYTGTTLAFTPTNAGGHTFYFIAQNQRNDAFATTNFTVTILGGGGEVEPIEIVAINSI
                     VSGGGQVSFTAEIADLPEGVTSLPIYAADQVVNGKWNWSFVTNVVVGSTTVNATLVLD
                     EVPTRVITLGKPPVGMD"
     gene            20981..22099
                     /locus_tag="PKI64_02605"
     CDS             20981..22099
                     /locus_tag="PKI64_02605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD99930.1"
                     /translation="MKVPLLDLKPQYAQIQAEVEPEILAICKSQAFILGPKVQECEAA
                     VAAYCGAAHGVGMTSGSDALIVALMAEGIGPGDEVITTPYSFFATVGAIARLGAQTRF
                     VDIDPVTFNIDGRKLADAVTPRTKAIIPVHLYGQMADMDAVMAVARQHKLVVIEDAAQ
                     AIGAEWQGRRAGSIGDYGCFSFFPSKNLGCFGDGGMLTMQDPAKAHRATILRNHGSEP
                     KYYHKLVGGNFRLDALQAAVVTIKLKYLDGWTAGRQANAARYQKLFAQTGLLTSGKVQ
                     LPAAVTNRHIYNQFVIRAQNRDGLIAHLRSRDIGTEIYYPVPLHLQECFRDLGYKAGD
                     FPESERAAKETLALPIYPELSDAMAARVVEVIDEFYRG"
     gene            22177..22722
                     /locus_tag="PKI64_02610"
     CDS             22177..22722
                     /locus_tag="PKI64_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99931.1"
                     /translation="MKKVAMMALAVVFASAMSSQAWWIFGKKAAADQPAVEQQVDQKA
                     CAEKAKADCAAKKADCKAQKADCKAKKADCKAKGECKCTKDNANCKNPNCPMKQADGK
                     APKADCKAKKADCAAQKADCKAKQADCAAKKAEKQAECKGKKAEKKADAPVKKAERKG
                     KKAAEKAAEAVEAPAAVEAAE"
     gene            22804..23622
                     /locus_tag="PKI64_02615"
     CDS             22804..23622
                     /locus_tag="PKI64_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOD99932.1"
                     /translation="MSKDISRLVDGWPYEPGEVRARWIEADDGSRKLQLRLDLGIFQM
                     EPTGRPDGTCPRGAISLRDFYIEEEMRSPDEKLPYKLDNDACAELQLEAMQYYYRIMA
                     FYALGEHKSVVKDCEHNLDIIDIVSDYAADDETAWQFNQLFPYMRMMLARAVAEVAMS
                     RDRFDRAARAVKDGIRQIEQFFAENYEPQNADGTPAPPPAEVISLRELLADIERRRPK
                     SEAENLRAELARAVELENYEKAAFLRDQLKRLQGLATRPPDKKRSRSESKENRS"
     gene            23619..25319
                     /locus_tag="PKI64_02620"
     CDS             23619..25319
                     /locus_tag="PKI64_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99933.1"
                     /translation="MNARLAIGWALAIGLVIPVAHGAPALYQGEGSWGAITGASADIA
                     PPALGWVFQDGAWLGRLPANADSLSVGAERLAIADARLAPPPTVVLPKERNAFGPCAV
                     RSLSMRYDEAARSRRWTIIFSDDPALEPAFFLPSLVVRPVLELEDIQTGQRWPLQPTR
                     RGREGGRFPTRHDPRYYGGTLDDDDIEWSMMVIPLENGRRILQGRIMLQKSDTRLFRL
                     RVGLVTGTPGKAVLQDENPPAVVSVSDGQAVGLFPDLAEPRRSRVVAIPGQGPAIEMD
                     LAVTKATGNFPRRVTFSFEAETWPSTDVAAATQEAVAKLARTGGAVPVPDAVLRAGPD
                     ALPAYDLICHRLTFPGGFANAQDAFQFLQLNMSSLFADSADSASAYLSVAHTAAGEPL
                     VELIPDGAQVAVNPDPDLEALLELGQNRGQTLLASINRQRAGAVWIRLGPFADDLDCS
                     ARGLYMADYPAAWPAGVAKPGVVLGHAAAEWVSSLSCLLRKANTCLLVEDTSTLAPYT
                     TVYADALVCTSADPAEMRRQHALAGPRPVLWAARQSTAAANALAHDLGFVTWRQINDN
                     "
     gene            25323..26363
                     /locus_tag="PKI64_02625"
     CDS             25323..26363
                     /locus_tag="PKI64_02625"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOD99934.1"
                     /translation="MLENPVGRLLSGAAATLEKSARAWQRQAVDWDMLAEAPPPLPPR
                     MQTTTEREVTVRGRGTFFGKSIRTLTLCPTDMEGWWFERTDLPDCLPVRCSIRNVWTT
                     GNVVSNIVLRSGSPHNYIRMAEHMIALRMGLGIDNLLIKIDSGDPPLFDRGSTELVQA
                     LRSAGSRSTTRSQPLVTVREPVTVVSGSGSFLTLAPADPACPRLNIDAAVDFKTAIGK
                     QRIRFAVDPERFAFGATARTNSSALKMLYCRTIGLLFADIRNLGYSLDNLLVAGRFCY
                     WNKPLLKHNGKSLEAVWHRATLDLLAAIALIDEGLFVGDIISYKSGHGLDVEMVQRLY
                     QEGLLQPLAAAK"
     gene            26389..27093
                     /locus_tag="PKI64_02630"
     CDS             26389..27093
                     /locus_tag="PKI64_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOD99935.1"
                     /translation="MILLLLGLATMVHTAAGQATLPTSYTGPWYLAFPPDGWTFSGLG
                     GPDYIPGYDPAQDGAAKFTSTNSAITIHFAQPPAALSYWICGNMYTGGIFRVEQSVDG
                     TAWTTLQDYTALPNASNHVTHAVDPTARYIRFHYLDKFTGNVGVDGIAITRLESLIPR
                     FSRFVYENGTITAHLDVSFAECGYVLESTPGLTTNTLDWYFEDERYGTDGPLVMPANP
                     YHSGPTRFYRVRQVCP"
     gene            complement(27137..27388)
                     /locus_tag="PKI64_02635"
     CDS             complement(27137..27388)
                     /locus_tag="PKI64_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99936.1"
                     /translation="MTAEQENKKPRKAGKIIVWIVASLSAGMANTFPSLSFACFASLR
                     DSSSFPAQAQSTKNQVPPAFFLLGFSDPAADTTAAYIKS"
     gene            complement(27659..27958)
                     /locus_tag="PKI64_02640"
     CDS             complement(27659..27958)
                     /locus_tag="PKI64_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99937.1"
                     /translation="MKKLLMMLGLAVLMLGLTGCEDYDKQPLEFVNASTYEVTVRSLS
                     TEWGDFILPVGASKKMTGIRDVDYTYYPETRVKLGFASTDRYIVFVNESADRKSW"
BASE COUNT         5592 a         8170 c         9018 g         5239 t
ORIGIN      
        1 ggagcaacca aggacaacat gagtggcgta gagaaaaaat ggaatcaggt ggcggtgctg
       61 ctgggcgggc cgtcgtcgga gcgggaagtc tcgctgaggt cgggcgcggc ggtggcgaag
      121 gcactgcgtg cgcggggcta tgacgcgcgc gaagtggagg tggataagga atgccgcttt
      181 gtgctgccgg cgggcaccga ggtggcgttt gtggccatgc acgggcgctt cggcgaagat
      241 ggcggcgttc aggccgtgct gcgggcgcag ggcgtgccgc ataccggttc cagccccgag
      301 gcgtgcgcgc gcgcgttcga caagtcgcaa agcaagccca tcatggtgca ggcgggcatt
      361 ccgacgccgc cgtacgagtt gctgcgcgcg ggggagccgc ggacgctgcc gctgccggtg
      421 gtggtcaagc cggtgcggca aggttccagc gtgggggtgt cgcgcgtgtt caaggaagag
      481 gagtggccgg cagccatcgc gctggcacac cgcctcgacg agattgcgct ggtggaggag
      541 ttcattgacg gccgcgagct gacggtgggc gtggtgggcg atgtcgcgct gccggtggtg
      601 gagatcatcg caccggatgg taattttaat taccgtgcca aatatacgct gggagtgtcc
      661 aagcacgtgg tgcccgcgcc gctgccggcg gagctggccg ccgcgtgtcg cgacgccgcg
      721 ctggcgctgt tccgtgcgct aggatgccgc ggcatggcgc gcgtggattt tcgcctgcgc
      781 tccgatggcc gtttctatgt gcttgaactc aacaacattc ctggcctcac cgagatcagc
      841 ctgctgcccg atgccgcgcg cgcccacggc tggagttttg aagaactttg cgaaatcatc
      901 ctgaatatgg tagactgctc cgccacaaca gaaaaaggag cataaataga ttatgaatgt
      961 attacgtgtg atcgccaacc cgaagccggt ggagcaatcg gccagcttga aaatcgaagc
     1021 cgccttcatg gcggccctgc agcaaaagca tcccgatgcg caggtcacca ccatcaatgt
     1081 ttttcaggat gatgtgccgc agttggatgc cctcctgctg ccggcattct ttggcgcgcc
     1141 gccagccaat gccgagatcg agcgccggcg cgaccgccag cgcgagctgc tcgcgatgtt
     1201 tctcgccgcc gatctggtgg tgattgcctc gccggtgtgg aacttcaacg ggccgccgca
     1261 gttgaaggcg tttattgacg ccatcatggt ggctggcaag accttcaaat ataccgccaa
     1321 cgggcccatc gggctcgtgc cggaaaagaa ggtcgtggtg tgctacgcca gcggaatgct
     1381 ctacgaccac ggccagccgc cgcagcaaac cctgacgccc atgctgacca cccagctcgg
     1441 cttcatgggc atcacgaatg tgacgctgat tggcgcgcaa gggcagggcg cgggccgcga
     1501 aaaggctgcc gccaccctcg ccgcggccat cgaaaaagcg caagccgccg ccgcgcaatt
     1561 ctaacgccgc ccgctcgtgc gcaggccgcc tccccggcgg cctttttctt tcggttgttt
     1621 tgcccgcact ggggaggatc gtgatggatc agaagccccc tcttgtatgt gacccttttt
     1681 tgcggtcaaa ccgtggggtt gcagccagca tcaaatcaac ccattttatt ttgcggattc
     1741 tgacatgcca tccagagttt ataaactctg gatggtttcc gtattagtct tatctcattc
     1801 tcatacatta taatatgtat aaaatattgg ctatttataa tcggctgttt tgcaggtgcc
     1861 atccagagtt tataaactct ggatggatcc gacgtgaggt gttttgtgtt tttatccgct
     1921 cggtgcggac ccgcatgtag gtcgttgaaa ggtgccagaa accatcggcg cgatgattct
     1981 gaaaaccaca tcatttatgg tcaagttctg ctggcgcttc gttggcaatg catggcagtc
     2041 ctcggctcag gcacctctac ctgatcccga gcaccgtcac cccaatcggc gaagaaactt
     2101 tttggccgta gcgcgcgcaa agaaaaagtt ttcgcggggg tgggggtgtg gcatagtagg
     2161 cgcctgtttg tttggtaaat ggtgaggttt acgttggcgg cattgaagtc caagcggcgg
     2221 ggcaaccagt cgcgcgaaag catactgcgg agcaaagcgc gttccagcgc ccggcggcaa
     2281 gccgtgcgcc ggggcgtgac caccgccgtg gtcgcgctgc tgggcgtggc tgccatcggt
     2341 gccgcgctgt ggtggggcgg gcaggcggcg tggcaaatcc tgttcaccga aaacgagttt
     2401 tttctgatcc gcgatatcga agtgacgacc gatggcgcgc tgaccatgca ggagattctg
     2461 gatgccgcgc aggtgagcaa gggccagaac ctgtttgcgg tgcagccgga caccatccgc
     2521 gaggcgctgc tgggcaaggc catgatcgcc agcgcgcagg tgggccggcg gctgccggat
     2581 acgctgctga tcgaagtggg cgagcgcgtg gccgtggccc ggctggggcg ctcggatgcc
     2641 ggcagcccgc tggcggtgga cctgaccggc catgtgctgg ggcccagcag cgtgcgcgcc
     2701 agtttgcccg tgattctcgg cgtgcgcgat aagggcctca agcccggcga tgtggtgcaa
     2761 gaccccatgc tggccgaggc gctgcaagtg ctcgagattt gcaatctggt ggccatgcgg
     2821 caggaactca ccgtggccac catcgaagtg ggccaccccg accgcctgac cgtgggtctg
     2881 accaccggcg aaaccgtgtt gctctcgcgg aacaagctgc gtgaaaaatt ggaccggctg
     2941 cgcgccatgc gcatcgaagc gcggcggcgc gggctggatt tggcagtgta tgacatgacc
     3001 gtggaccgca attttgtggg ccgcccggcc acgggcgcgc cgccggccgc ggccgcggcg
     3061 ccggcgtggt aggggcgcgc ggagaaagga acgtggaaga atgaagttgg aaccgatcgt
     3121 ggcgctggag attggcacga gccgcgtgcg cgtgtttgtg ggcgagccgc gcgaggatga
     3181 aggcctcatt attaccgccg tgggcgattg cgccagcagc ggcatgcgca agggcgaagt
     3241 ggtggacttc aacagcctgc acccctgcgt ggaagccgcg ctgcgccagg ccgaggaggc
     3301 cagcaatgtt tccatccacg atgtgtatct ggcgctgtcc ggttcgcaaa tcgaggccat
     3361 gcccaaccat gccgaaatcc aggtggatgg cgaaatcacc gcggagcata ccgcacgcgt
     3421 gcgcgaactg gccaaggccg tcactctgcc ggcggaccgc gaaatcctgc acagcatcat
     3481 cgggcagtac acgctcgacg gtttgcgcgg cgtcacgccc gagggcatgg cggggcgcac
     3541 gctggaagtg aatgtgctca ttgtctatgg cgtccattcg cggatgttca acttggtgcg
     3601 cgtgattcaa gagctcaaac tctccctcgc gggcacgctg ttcagcggcc tgtgcgccgc
     3661 ccaggccgtg ctgacccccg aacaaaaaat gcagggcacg ctgctgattg acctcggcgg
     3721 cggcacgacc gattacgtgg tctatgccca ggcccaaatc gccgacgccg gcgtgctggc
     3781 cgtcggcggc gaccacgtga cgaacgatat cgcccgcgcg ttccaagtga ccaccgccga
     3841 tgccgaggcg ctgaaaatca agtatgccga tgccatggtc agcggcgccg accgcacccg
     3901 ctgggagccg ctgccggctg cagccggcgg cccgccgcgc caattcaagc gcatggatat
     3961 gcagtccatt gtgcacctgc gcatggagga aacctttggc ctgatccgcg agcgcctcaa
     4021 ggcccgcaca cagttgtcgt ttttgagcgg cggcgtggtg ctgaccggcg gcggcgcgca
     4081 ccttaaaaat gtgacgaccc tcgccgagaa ggtgttcggc ctgccttgca ccatcggcgt
     4141 gccgcgcaat gtgagcggac tgaccaccaa ccttgccctg ccggaatatg ccaccggcat
     4201 cggcgcggtg ctgcaaggcc accgcaatgc gctcaatgcc gcgcaacagg ccggcggcgt
     4261 gtggaagaaa atcggccgct ggttcggcgt gggctgaaga gaggatgacg atcatgaccg
     4321 cacaaccgga gaatccgcaa gcccaggccc ccgcgccccg ctgccgtatt ctggtggtgg
     4381 gcgtcggcgg tggcgcgtgc aatgtggtca gccgtgcatt ggccggctgg aacgacgcgc
     4441 cgctggtggc gggcatcaat accgacgcgc ctgcgttggc gcaaagcgat gttgccctca
     4501 aactggccat cggcaaaaac gttaccaagg gcatgggcac cggcggcgat gtggaagtgg
     4561 gccgcttggc cgccagcgaa gacctcgatg cgctgcgcaa cctggtggcg gggcacgacc
     4621 tgctgctgct ggtggccggg ctgggcggcg gcaccgccac cggcgcggcg ccggtgctgg
     4681 cgcgcgtcgc gcgtgaggaa ggcctgttgg tgctggccta tgtcaccctg ccgtttgcct
     4741 ttgaaggcgc gcggcgccaa gagcaggcgc aggagggcct ggtcacgctc aagcgcaacg
     4801 cggatgccgt ggtgtgcctg cccaaccagc gcctccattc cctgctgccg cccgatacct
     4861 ccctgcccga tgccttcgcc tttgtggacc gcatgatggt ggcgggcatc cgcgggttgt
     4921 ggaccctgct ggcccagaac aacatgctca accttgattt ctccgacgtg caaacgctgg
     4981 tggaaaacag cggcaacgaa tgctgctttg gctatggcga gggcgccggc gccggcaaaa
     5041 ccgaacaggc cctgcaaagc ctgctggccg gtcccatgct ggataaaggc cgcgtgctgg
     5101 ccaactccgg cgccatgatc ctaggcgtga tcggcggccc ggacctctcg ctggcggagt
     5161 tgagcaccat ccatgaacgc ttcaccgccg cgtcgcggcc cggcgcccgc atctcgctgg
     5221 gcgcggccgt gctggatgac tggcccgggc ggctcgcgtt gattgtgctg gccgttgaac
     5281 attggctgcc gccgcccaaa cccggcgcat tggtgattga agccggccat accggcccgt
     5341 cggccgagcc cgccggacgg cgccgcgcgg gccgggggca atccgagttg ccgctggcgc
     5401 ccgccccgga ccgcggccgc ttccagaatg tctcgcccac cttgcacgat ggcgcggatc
     5461 tcgacatccc cacctatatc cgccgcaaca tccgcatccc cacctgaccg cgcgccttcg
     5521 ccgggaaagg cttgctttta tttgccggaa cggcaagagt gccccggctt gaaacacagg
     5581 agaaaaaaat agatatgaag aagatgacat ggttgagtct gttggccacg ttgctggccg
     5641 tttccccgct ggcctttggc gcggccctcg tcaccaaggg cgccaacgaa ttgggtttgg
     5701 aaggcgattt ggattttgcc accgccgtcg gcacccggct ggaagccagc gccaaatatg
     5761 cgtatttctt ctgggaccgg atttcgctgg gcgtgcaggg cgtggtgttc aacaacgacg
     5821 ccatgcacca gttcgggctg ggcgtgaacg cggaatacaa tttcacgctg tcgagcaact
     5881 acaagccgct gttcggcacg gatttggtgc ccttcctcgg cgccgccatc agctaccgcc
     5941 acgccaagct gttcgatgaa aaggaaagtg cggtggtctt tggcggcgaa ggtggcgtga
     6001 agttcttcct gaccgacacc acggccatca cgttgtcgct cgtgggcgaa ctcgccaccg
     6061 aagacatcta tgatgacgac ctcgaagcca ccaacaagga cctgagcctg cggctcggca
     6121 tgcgcttcta ttactaaccg ccacggcggc tcggcgcgga cgacctccgc gcctttttta
     6181 tgcccggcgg attcggttga aatccgcggg cgggtatagt attcatttgc ctgttcgcat
     6241 ccggttgcgc cggaggcaac accgcggagg gatggctgag cggcttaagg cacccgactc
     6301 gaaatcggac gggcgtgatg agcgtcccgt gggttcaaat cccactccct ccgccatttt
     6361 tattttgttt ttttatgttg gccatgccga cggcaggaga ctgataatga aaaataaaat
     6421 agttatcggc ggggttgttg cgttcttgtg cggcctgata ttaaatgccc atgctcagtt
     6481 tggcaatgcc cagccgcggg tgggggatcc caactggaca actgatgccc atggtcgcgt
     6541 cgtgccccgc atcggcaatc ctggctggac tacggattcc cacgggcggg cggtgccccg
     6601 cattggcaac cgcgaatgga caaccgatag ccggggtaat cccgttccgc gcattggcaa
     6661 tcccggctgg accaccgatt cccgtggccg cgccgtgccc cgcgtcggca accccagttg
     6721 gaccaccgac agccacggca accccacccc ccgcatcggc accggctatt gggatttcta
     6781 atctgcagcg tgcggtattt gttgatttgc gtcgctcggc gcgttgtgtg gaggggtgaa
     6841 tgagacgagg gtattgttgg attatagtgg gcatggtttt gggcacggcg tcttgtcatt
     6901 ggccgggcgg tcacaccatc gcgcaagagt atcctgatgc cgtggccagc gatccggcgc
     6961 gcttgggatg gatggttggc gcgccgccgc cgcccgaccg gatggtgcgc tttgatgatg
     7021 gcagttattt tcgcttcccc gcgctccggt ggagcgtcgc caactttcgc caactgatgc
     7081 caaccgtcaa tgtgtcgcgg gggctgggtg cgccggtgtt gttgccgcaa gcgctccgtg
     7141 atgatctgga tgatgtggcc ttcagcccgc ttggcgggga cggcacgatg acgtgggcag
     7201 aatcgctgcc ggccaactac acggatggca tcatcgtgtt gcacgaaggc cgggtggttt
     7261 atgaacgata tttcggcgta ttacgcccgg aagggcagca tgcggccatg tcggtcacca
     7321 agacttttgt ggggacgctg gcggcgttgc tggtggccga gggccagttg gatccggagc
     7381 ggaaagtcgc cacttatgtg ccggagctgc aagcatccgc gttcggtgat gccaccgtgc
     7441 gccaagtcat ggatatgacg acgggcatcc ggttcagcga ggactacgcg gatcccaagg
     7501 cggaagtctg ggcgcatgcg gtcgccggca atccgttgcc gaaaccggcg gattataccg
     7561 gtccaaggtc atgctacgag tttttgcaaa cggtgcagcc ggaagggacg catggcgagg
     7621 catttcatta ccggaccgcg aacacggatg cgttgggctg gattctcgcg cgggtgagcg
     7681 ggcagtccgt tgcccaactt ttggagacgc ggatttggcg caaattgggg gcggagcagg
     7741 atgcgtatat gaccgtggat gccaccggta ccccgtttgc cggcggcggg ctgaacgtgg
     7801 gattgcgcga tttggcgcgc tttggcgaaa tgatccgcaa cgacggctat tacaacgggc
     7861 agcagattgt ggcgcgggag gccatcgcgg acatccggcg tggtggcaac cgggaagcgt
     7921 tcgcaaaggt tggctatgat ttgctgcagg gttggagtta tcgctccatg tggtgggtga
     7981 tgcacaatga agatggcgcg ttcatggccc ggggcgttca cggccaagcg ctttacatca
     8041 acccgcgcgc gcaagtggtc attgtacgtc tggccagcca cccggttgcc gccaatgcgg
     8101 cgaacgatcc catcacactg ccagcctttc aggccatggc ccgccacctg atgcaggaga
     8161 aagaatgaac ctgatgttgc tgtcaggcaa agcatggttt tgggtggtgc tggtggtctt
     8221 agccggtgga acgtatgcac tggcgacccg ggccaatctg aattttcgtc accggccggg
     8281 ggatgtgctg gatcgtttgg agggtgtggc catttattac aatggcggga ttcacatgac
     8341 gcatggccgg aatctggccc cggatggata taatctgggc atccgctatc aatgcgttga
     8401 gttcgtgaag agctacttgt acgagcggca cgggcaccgg atgccagatg catatggtca
     8461 tgccaaagat tttttcgacc cgcggctggc tgatggccaa tggaacgcgc ggcgcggcat
     8521 gacgcagttt gccaatggca gcgccaccaa acccgcgact ggtgatttgt tggtgatgcg
     8581 gccatggctg ttcaatcgct atggccacgt ggccattgtg gcggcggtga caggcaacag
     8641 cgtggagatc gcccagcaga atccgggtcc atggggcagc acccgcgcga tctatccctt
     8701 ggctatccgc gaggggcgtt ggtttattgg acaacgccgg gtcctcggtt ggctgcgctt
     8761 gccgacagcc gaatagcaaa acgaaatgca acgcaaacgg ccgccagcgc ggtcatgatg
     8821 tcagccgccg ttatatcggc ggcgggatcg aaacaaaagc ccaacaggaa cgtaggaaac
     8881 tcagaaaagc aggagagaag aatttttaac gctaacgacg ccagcgacgc caaaggccgc
     8941 aagcatggcc acaaggcggg aaggggatta tctacgtttg gagcgggtgc gatatttggc
     9001 cgcaggttcc gcaatttggg gttcaggaac cgcgagtgct gggtggtggc gcgcaacaac
     9061 aggtggcacc aaagagcgca ggtagtccac tccaatccgg ccgtcgcgaa tggcgcagag
     9121 caggacattc atcgagcggg aggggcgttc ggcgcccgat tcccagcgcg tccatgtttt
     9181 ttcgccaagt tgcagaagtt cggccattcc tttttgggtg gccttgattg agcggcgcaa
     9241 ggcgcgaatt tgtgcagggg acaggatgcc tagaaagcgc gccttggtgg attccagcag
     9301 ttcgcgagcc tcaccggaaa gaaaaatctc gccggtttta ggatccttcc atgcagggac
     9361 atctttccag ataatttccg cgatgccatc gcctgtggtt tcagggatat agaccggctg
     9421 gcgggaagtg accagtaccc agccggcgac aggtgctttt ttcataagga atctccttta
     9481 cgtgggatgt ggcttgagaa gatgatgatg attttaccat tggggagcag gccgatttta
     9541 cccagcactc gactctggtc gaaaaagaac caaaactcgt atgcatcgca cgctggccgc
     9601 atatcagcaa tgtgccggcc gtgaatatcg gcccggagag cctgcgccat ggcttgatgg
     9661 cgcgcgaaca tgaacgcatg aggaaacgcg gcgttccaat cccggtcagc ggtctctgtg
     9721 ctgatgattc gatttttatc gcccgattcc agaatcgcaa tcacagcgtt acgccaagca
     9781 gcgggaatga ggagtaactt actcatgccg tgaatgtatc aattgataca ttgaaagcaa
     9841 catttttcaa cgccggcggt acacggctga aagcatggaa aaggggagga aggtgggctg
     9901 ttttggtgtg aagctacggg gtggcgagaa gagaggtttt gagggattct tgggcggggg
     9961 tggcgccgag ccagatggca aagagggctt gcttgaggtc gaggccgggg atggtggcga
    10021 gcggggtttc ggtgccgcca gcagtgcggt aaagggtggt cagggcgcca tcggcgtagc
    10081 gcatgatgac ggtatcgccc ttcttgaatt cggtattcgc gaattggtca aggaaggcgg
    10141 gggtgttggg cgaggtgtaa ccgctggcgg cggctttggc gaagccttcg gtggtggttt
    10201 ccacaaagcg cttgcgggta atgagcgagc ttttgagggt gaggtggaag gccatgggtt
    10261 ggtcggcgcc gaggatggcg gggccatcgg cggcttgaag ggattcgggg acgtagaggg
    10321 tgagttcgta tagcgcgccc aggatgggtt tggtgcggat gccggagccg cggaccacga
    10381 gggattcgct ggcggtggcg gcgaggacga gggcgagggt ggcgatggca atggcaggca
    10441 ggcggtggag tgtcatggga tccttccgtg ggttctaggg tttggttaga tgagcaggtt
    10501 ttgggcgatg gcggtgcgag tggtttcggt gagttggtgg gtgtcgaagt tttccggcga
    10561 gatgggcggg aggaagcgga cggtgacccg ggtgccaagg cgggggaggc gggtgccgcg
    10621 ggcgagggct tggtgggcgc cattgatagc aacggggata accgggactt gcacttcctt
    10681 ggcgagggcg gcgaaggtgg tcttgaagtc gcccaaacgg ccatccgggg agcgggtgcc
    10741 ttcggggaaa atgagcaagt tgccgccgct gcggagggcc tgagccatca tttgcagcga
    10801 gatttggaca tcgcgcccga catccaccac gatgacgttg ctgttgcggg caagccaagt
    10861 gagcagtcct tttttgacgt gcttgcgctt ggcgtaatag aaggtgcggc gcaggaagcg
    10921 gttggtgagg aacatggaga cgaacagacc gtcaatatag gactggtggt tggccaccag
    10981 gatgcaggag ccctgcgcgg ggatgttctg gcgtccttcg gcgcggacgc ggaacagcag
    11041 gcgcaggagg gtgccgaaga ggtttttcag gaaggggtgg atccagcggg agcggggcag
    11101 ctcaagtggt ttttcgtcgg gctgggcctt gaggagggca tcccagccgc cgccgccttg
    11161 acggaagaac agacgctgtt cgtcggcatg gcgcgcgagg gccagcaccg tgggataatc
    11221 ctgaaaagcg gtttcgggga gtttgatgcc gaaggttttt tcgatgaaca ccagcacgga
    11281 gaggcgggcg agggagtcga gggcgaggtc catttcccaa tgggcggtgg gggccacgcg
    11341 ggggcattcg agctcggtgc gcaggaagtg gtcaaaggct tcgtaggcgg ggccgaggtc
    11401 ttcgggcttg ggggcggggg cggagccatt gctgaaggtg ccggtggcga ggggggggag
    11461 ctcgtggcgc ttgagtttgc cgaggcgggt tttagggagg tcggcggtga cgatggtgag
    11521 ctgggtgatt ttcttggcgg ggggggcaag gcggttatag ggctgcaaaa gctgccagcg
    11581 gaagtgttcg gcctgctggc tggggacgcc ttcgaggaaa ccggggacag gctggatgag
    11641 cgcgtggaga agatcatctt gcaacgtgac ggcgacatcc ttgatgtcgg gcgagagggc
    11701 gaggagtttt tcttccagtt cggcggggtt gaccttttta ccattgggca acacgatgat
    11761 ttcttttttg cggccggtga taaagaggta gttctcgttg tcgaggtagc cgaggtcgcc
    11821 ggtgtggagc cagccatcct gaatggcttc ggcagtcgcg gcggggttgc gccaatagcc
    11881 ggggaagacg tgagggccac ggacgagaac ttcgccttcc tcgatgcgga cggaatcggg
    11941 caggcagggc tggccgcagg aaccaaggcg cacggctgag gggcgcggga aggtgatcat
    12001 gggggcgcat tcggtcatgc cgtagccttc gagaacctga aaacccatga tggtcatatc
    12061 gcgggcgaca tcgcggtcga ggggggcgcc gccggagacc atttggcgga ggcgtccgcc
    12121 aaacttcttt tggacgggat agaggaggac gcgggagacg cccagacggt taatgcgggc
    12181 ggaaaggcgg tagagcaggc ggccgacggg ggatttgaac aggttgtcga gaatggcctt
    12241 gcggaaaagg gcatagaggc ggggtacgcc gatcagcagg gtggcgcggt gccggcgcat
    12301 ggtgccgacc atgtcggcgg ggtcgaggga gtgagcgagg atgatggtgc cgccggagta
    12361 gaggggggcg agcaggcagc cgacgagcgg caaaatatga tgcgcgggga gcagggcaaa
    12421 ggtgcgggtt tgcggggtaa agatggggac gcggtcgcaa acggattcga ggttcacctt
    12481 gatgttgccg aaggtcagca tgacgccctt gggcgcgccg gtggtgccgg aggtgaagag
    12541 gatggccgcg agagtgtcct ccgcgccagc catgaggggg gtgtcggcga cgcagccgga
    12601 gggaatggcg ggaaagtctt cgttatcgaa gcaaaccatg cgcgcgttga gggccgggag
    12661 ggtgccgagg gcttcctgca ccacgggggt ggttttggcg gaacagaaga tggccacggg
    12721 ctcggtttgg ccgaggatat acgccagctc agcggcaggg ctgaaggcgt ccaccggcac
    12781 caccacgcag cgattacgcc agatggacag cagggcggag acccattcgg ggcggttttc
    12841 ggagaaaatg accacgcgct caccgggcga ggaaaaatag agcgaggcca gcgcggctga
    12901 gtgggtcagc aagtcggcat aggagatggc ggtatctttc caaagaatgg ccggcttggg
    12961 tagggggcgg agcaaggcga aggcgggcag ttggaaggtg ggggcattca ttcggtcggg
    13021 accatggggt tgggtgcttg gtgtccttgc tgttctgact ctaggggggc gactttaccc
    13081 cagccgggcg agggaaacaa ccaacaacac gtctgggatg ggcatttttg cggaatgggg
    13141 gggcgggtag cgggttgttg ttgtatcttt gcgcttgtgt tcgggcgcat gggaagtata
    13201 acgttttgcg cgtcacgctt tgccgggtga cgccgaattc aagaccgctt aaaaaaggcg
    13261 aggagcgagc catgaaatgg atgaagttgt tgggtgtggt gggtgcgatg gcggtgctgt
    13321 cctggacgac agccggggcg aacgaggaga ttgcctcgac atggtacggc gaccagccgg
    13381 acgtgtatgg gctcaagtcg cgcgacacgg cggggaataa ttcctatgcc tatacgccga
    13441 gcccggagca atggcgcgac aaaaacatct accagttgtt caccgaccgc ttcgcgacgg
    13501 atggcgtgga ccgcctggcg ggctacaagc cgagttggac gtgtgacggc aagccgtatc
    13561 cctacaaccg caactaccac cacggcggca actggaaggg tttgcagggc caactggatt
    13621 atctggccgg catgggcgtg acggcggtct ggatttccgg cgtgcagcaa aatgaccagg
    13681 gcaaggatcc caattatacg ccctaccacc agtatcacgt tgaaaacttt ttcaagtgcg
    13741 acccggccat gggcaccttc caggacctca aggacttgat tgacgactgt cacgcgcgcg
    13801 ggatgtatgt gattttggat gtggcgccga accacatgtg cgacaaaaac ggcatcaaca
    13861 ccggcaacga aaatgatgac aagcaatact ggcccaacgg ccacggttcc ttcggctggt
    13921 ggaacgaaag caacaagcac gcgctgccct tcaacaacct gaactatttc cacccgaacg
    13981 gcacgatcaa ctgctgggac tgcttccccg aacaaatcaa cggccaattc aaaggcaccg
    14041 atgacctgaa caccgagcat cccgatgtgc agtctatttt ggccaaggcg ttcaaaaacc
    14101 tgattgatgc cacggactgc gacggcttcc gcgtggatgc catcaagcac gtgccctaca
    14161 actggtgtcg gacgtgggcg cagaacattc gcgaccatgc ggcgtggcgc ggcaaaaaca
    14221 acttcctgat gttcggcgaa ctgttcagct atgacaacgg cgcgctggcg agctggtgcg
    14281 cggatggcta cggcttcaac tcggccctgc atttcccgct gatgcaggcc atgaaaaacg
    14341 tcttcggcta cggcgcgggc tcggatcagc ttggctggga aatgggcgca atcaaccagt
    14401 acggccaagg caaggaaaac gtgattgcgt ttttggacaa ccacgacgtt gaccgctttg
    14461 cctgcacatt cggcggaggc gatgccgcca ccgccaagaa gattatggag ccagccatga
    14521 cgttcctcta catggcgccg ccagtgccgc tgatttatta cggcacggag cacatgttca
    14581 accagggcgg ccaccccaat ggcagcaacc gcacgtggga caaccccgat gacggcgact
    14641 ggcagcgcga gtgcatgttc gacaaaggct tccagccnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnccc accagtttca cttccgccgg caacggcaac attgcctttg
    14821 gctcgaccaa aatcccgggc aagggcagca agattttcat cgccaatttc gtggacaaca
    14881 gcaacgacgt attcggcggc ggcggtggca cgccggtggg cctttgggcg cgcaacacct
    14941 atggttaccc gaccgaaact gccacgacgg ccgacaccat atacattaac acggaagccg
    15001 gccccgccag cgtcaccaac aagaaggtgt acttggtcta tggcttcaac aatcccagcg
    15061 gcgcttggcc gaaggtagag atggagctca atgaagaatg ggattccaac ggcggcaact
    15121 ggttccacta cgacctcggc aacctgacga ccggccgact cgattatgtg attgccgtcg
    15181 agacggtgga agacggcgtg accaacacct tctgggataa caatggcggc ggcaactaca
    15241 ccatgcagat ttatcccgca ccggaacccg tcaatttctc gttccacagc gtggccgcga
    15301 acccggtgaa tccggaaccg gagggcagcg tgacggtgac cgtgattctg cgcaccgcgc
    15361 cgggaaccaa catcagcgca ctggcggcac gggtcggcta tgacatcttt gacccgaaag
    15421 acggcccggc gacgaattgg ccgacctacg ccatgacgcg gggcaacacc atcacgaacg
    15481 acggtaccaa cgtgttgagt accttcacct atacggtgaa caacggcctc gaggccggtt
    15541 atgtgatgaa gtactacatc gcggcgagca atggcgcgtc catcctctac gccaacaatg
    15601 gcggacagga ttactccgtg gtcatcaagg actggccggc tgtgccggcg gacatcgaga
    15661 tcagcacctc gaaccacacc acgcaggcca gcagtgccac tgtggccggc accgccggtg
    15721 cgaacatcgc cgatggcctc tattggacaa actccttaac cggcgacacc gcacaatttg
    15781 agctgactgg ccactggagt gcggccatcc cgttgaatgt cggaaccaac gtcatcacgg
    15841 tctatggcta cgaggcgggg accaccacga ccaacgccac cgacaaggcc tcaaattatg
    15901 cgacttggac gcacaattcg aaccaaggct ccggatttgg gaactggagt cttgtcggtg
    15961 tcgaagacta tgcgggccac ttcattgccg ttgggaatga aacatgcaac ctcaccaaac
    16021 ccgctttcgg gatgtgggcg cattcggaca acacatcgga agtgtaccgc ccgttcagcc
    16081 aggcgcttgg agcaggagac accttctcca tctccattga caacggcagc attgatagcg
    16141 gtcacagcac cggcatcggc ctacaatcag cctcagggga aaatctctgg gagttttact
    16201 ttgtcggcgg cgacttcacc tacaccatga ccggtggcgc gacggatatt ggctggacca
    16261 gcggcggact gacgattgaa ttcacgttga cttccgccag tacctacagc gctgtcatca
    16321 cgcctctggg cggttcagcg cgcacgtaca ccggttcctt ctcaggcact atcgcgcgtt
    16381 ttcatgcatg gcattactcg gctaacggtg gcgaagacca caacttctac ttcaacgacc
    16441 tgaagatcac ttccgcgagc ctgggcacaa agaagcagga tagtgtgacg atcacccgcg
    16501 aggatgacgg ccggccacgg atcgaagcca ccggcagccg ttccatcagc atcggcgaaa
    16561 acggcacggc gtttacgctt gggctttcgc tgacgaacgc cacggcggcc agttggagcg
    16621 cgtcattgac ggcgctaaat ggttcgcacg tgcagtcgtg gtcgaatgtg gcgggcgcga
    16681 cttggaattg gacgtggacc gcgtatgccg cgggcacgtg gcggctgcgc gtcaccgccc
    16741 atgccgccgg tggcgcagcc atcaccaata ccaccctggt gctgacggcc accgagggta
    16801 tgagctacac gctgggtaac gcctggcact gcccgaccaa taacgagccg tttgaggcca
    16861 aatatatgcg ccatccggtg accccgggcg tgggcgagac catcgaaatc tatgtcggca
    16921 attaccatcc gcagggcgac atgaacggtg gctcgctgca ctaccgctgg ggcaccagcg
    16981 gcgcgtggag cagcacggca ttgagttgga ccaacaacac caatggcgat gacaaccgct
    17041 actgggtcgg tacgcaagtg gtgccgggcg acaagaaggg cggcgtcttc cagtattatc
    17101 tggaggtcac ctataccgac ggctcgccaa ccaatacgtt tgtggcgatg gaccccgacc
    17161 agtccgccat gtacaagacc ttcaagcgcg aggcagccgc gcaggcgact ccgtttgaag
    17221 tgggcatccg ggatgatgcc ggcaaggaac cgggctatat ctggcatgga ggcgatatca
    17281 ccgtggccgg cgaggacagc gtgcagattg gcgccaagat cggctacatc aaggacggcg
    17341 cgaagtgggc cgataccgcc gtggtgcgct accgcgtagc cgaagatgcc aagggcgcca
    17401 agcaggcgcg cggcctcaag cgcatcctga agtccggccg cagcccgcgc tcgtggaatg
    17461 cgctgaccaa tcaggtcagc atggcctacg aaggcgaaat cccggattcc agccccaatg
    17521 gcaacgccat gcgctgggtg gctaccatca cggatagcaa cctgctcaac gagcgggctg
    17581 tgctgatcta tgaaatctac gccaagggtt ccaatggcgt ctggcggcag gcggaataca
    17641 acgccggcgg cggttcctcc acctttgaat accgccccta ttccgaaggc gcggatgacc
    17701 tgcaggtgcg cggcaacacc cgcgattatg acagcggctg gctggcggcg gattacacca
    17761 ccaccaagta cttcatcaat gaagccgacg cgcaggacac cgtcaccatc accgtgcgtt
    17821 acaaggcgcc cgccaacgcc cagaaggtgg agttgttcac caatctcggc cgccgagact
    17881 ttgccgatgc cgacatgaac aacgacggct ggccggatgg catcatcccg cccgccggca
    17941 acaccatcac ggcggaaaac acctataacg ttggcgccgg ctactggcag gcgatcccga
    18001 tgacggcggt gggtggcgcg tggcagaaga ctctcaccgt gaacaagtgc ggagcctatc
    18061 gcattaccgc gcgcttcctg gccgccggcg ccgccgaagg cgaatggacg tggtactccg
    18121 aagggcctga caactccctg cggcgcgatc acgctgtggt gatttcaccg cgcaaggcgc
    18181 ttgagcaaac gatgtacgaa ctcaatgtgt tcaccatcaa ggctacgaat accgcgcagt
    18241 cggggcgggg tacctttgag aagctttcag aaaagctcaa tgccggcagc ggcgcttacg
    18301 acgaatttag cttggcctat ctcaacaagc tgaacatcaa ctgcctctgg ttccagccga
    18361 tccacccctc cggtggcgac cgggtggaaa acgaccccgc cacttcgaat cgctactggc
    18421 cgggcagccc ctacggcacc aagaattatt tctcggtcaa tccggacatg tccgacagcg
    18481 cgaccgaatc ggcggcggtg gcggcgttca ccaactttgt gcgcctgtgc gaccgcgccc
    18541 gcggcgaaac catgcccgaa aatgaacgca cggtgaacac cgtcaacgtc atgctcgatg
    18601 gcgtgatgaa ccacaccagt tgggatgcga tctatggcga aggtctcgcg ttggccacca
    18661 acggcctcgg cgcagcggca atcgccgagc tgaccaatgc ctacggcaac atcgcggcgc
    18721 tcgctccgag cgagcgcatc gccaccaccg gcaagttggg catcaactgg tattccgcca
    18781 agaccaacta tgccaagccc gccacctatt acaacaccgc gcatgacaac gatatcggcg
    18841 tggcgccgga acgcgtggac ttcggcaagt gggatgacgt ggcggagctg ctctatggca
    18901 attactccac catggtccgt tgggatgacc gtgtgccgcc ggattacaac ctcaccgagg
    18961 aaaccagccg gatgtataac gaggacgacc ggtactatta caacgagatg ctgccggcca
    19021 ccaagctgct gtggaagtac atggcttcgt atccggaata ctggttaaag aagaccggcc
    19081 actccggcac caaccagccc ggccagaagg atggcaacgg cgtcctgctg gatgactacg
    19141 gcattgacgg cttgcgctgc gactacgccc aaggcctgcc cagccagttc tgggaatacc
    19201 tcatcaaccg cacccgttcc aagaagtgga acttcctctt catggcggaa tcactcgatg
    19261 gcggcctggt cggcaagcgc tcgaaccgcc atttcgatat cctgaacgaa aacatggtct
    19321 tccgcttcac gcaggacaag gtgagcgagc cggcgccatt ccaagccgcg ctcgaagacc
    19381 gccgcacgct gtacggcaac ggcatcatcc tgctgaacct gtccagccac gacgagccgc
    19441 tgccgttcgg tgatccatgg gccaccgcct cccgctacgc catggtttcc gccattgacg
    19501 gcgtgccgat gatcctgctc ggccaggaac acgccatcag tccctatggc ggccccgatg
    19561 gcgacaccat cgacaagtgg aaaggcttct acttcttcga acagaacttc gggaagtgga
    19621 tcccgcactt caagaagtgg aaccagatgt atgtgtggga tgacccgtgc tatccgtccg
    19681 gcgacttccg tgaatcacgc aagatggcgg aactctatgg ccgcatcaac aaggcacgct
    19741 tgatgagtcc cgcgttgcgc agcaagaacc gctggttcct caacgataat ccgcgcatga
    19801 tgaccgtggc caagtgggag accccgggcg ccaaccccaa cgtgcgtgat gccgtcattg
    19861 ccagcgtgct gttcatgaat gaaggcgatg gcggtttggt cggcaatgca caggtgtatg
    19921 atctctcccc atttgccgac aagctcggac tcgaaaaccg cgccgaccgg ctctataaca
    19981 tccgcaattt ggcggcatcg gatccggcag cgctgatttg gccggaggcg aaggccggtt
    20041 cggagatcat caccaacttc tacatcggcc tcgatggtgg catggacggc accaatgcca
    20101 atcctgccaa ggtctgggcc aatggcgccg tggtgcaata tctgcaattt gtggatgtga
    20161 cggcctacgc cccgccggtc attcaagttg gtaccattcc cacgaataag acggtggggc
    20221 agtccgtcag catttccgtc acggccaccg gcgatggcga gcccattgtc acgctcacca
    20281 atgcgccggc cggcctgctg aacagctata ccggcaccac actggccttc accccgacga
    20341 atgccggcgg ccacacgttc tacttcatcg cccagaacca gcggaacgat gcctttgcca
    20401 ccaccaactt cactgtcacg atcctcggtg gcggcggtga ggtggagccc atcgagatcg
    20461 tggccatcaa cagcatcgtc agtggcggcg gccaggtgag tttcactgcc gaaattgccg
    20521 acctgcccga aggcgtgacc agtctgccga tttatgcggc ggatcaggtc gtgaacggca
    20581 agtggaattg gtcctttgtc acgaatgtgg tggtgggcag cacgacggtc aatgcgactc
    20641 ttgtgctgga tgaggtgccg acgcgtgtca tcaccctcgg caaaccgcca gtcggcatgg
    20701 actaactccc cctgccaact gactactcaa acggcccggc cccaccgggc cgtttgcttt
    20761 accttgcacc accacattgc cagcggccta tgtgttgagc atgcctgcgg gccgcggtgt
    20821 ctcttgcggc gtttgcgtta aaattcattt ttttgttttc ttgctctgcg gttcactttc
    20881 ttcctgcttc cgcgttatgt ccgcgccgcg gtttttgcgt gatttgcggg gtggggcgtg
    20941 gcatagtggc tggccagtat tcacaggcta gggagaaacc atgaaagtac cgctgctaga
    21001 tctgaaaccc caatacgcgc aaatccaagc cgaggtggaa ccggaaatcc tcgcgatatg
    21061 caaatcgcag gcctttatcc tcggccccaa ggtgcaggag tgcgaggccg cggtggcagc
    21121 gtattgcggc gcggcgcacg gggtggggat gacctcgggg tccgacgcgc tgatcgtggc
    21181 gctgatggcg gaggggatcg ggcccggcga tgaggtgatt actacgccgt attccttttt
    21241 tgcgacagtc ggggccattg cccgccttgg ggcgcagacc cgctttgtgg atattgaccc
    21301 cgtgacgttc aacattgacg gccgcaaact ggcggatgcg gtgactccgc ggaccaaggc
    21361 gatcatcccc gtccacctct acggccaaat ggcggacatg gatgcggtga tggccgtggc
    21421 gcggcagcac aagctggtgg tgattgaaga tgcggcgcag gccatcggcg cggaatggca
    21481 ggggcggcgg gcgggcagca tcggcgatta cggttgcttc tcgttcttcc cctccaagaa
    21541 tctcggctgc tttggcgatg gcgggatgtt gaccatgcaa gacccggcca aggcgcaccg
    21601 ggcaaccatc ctgcgcaacc atggctcaga gccgaagtat taccacaagc tggtgggcgg
    21661 caatttccgg ctggatgccc tgcaagcggc agtggtgacg atcaagctga agtatctgga
    21721 tggctggacg gccggacggc aggcgaacgc cgcgcgctac cagaagttgt tcgcgcaaac
    21781 cgggttgctg acctccggca aggtgcaact gccggcggca gtgaccaacc gacacatcta
    21841 caaccaattc gtcatccgcg cgcaaaatcg cgatggccta attgcccacc tgcgcagccg
    21901 cgacatcggc accgaaatct attaccccgt gccgctgcac ttgcaggaat gcttccgcga
    21961 cctgggctac aaggccggcg atttcccgga aagcgagcgg gcggccaagg aaaccctcgc
    22021 gctgccaatt tacccggaat tgagcgacgc catggccgcg cgcgtggtgg aggttattga
    22081 cgaattttat cgcggctgac cagttgcgat cggcccaaaa cggggtatat tcggcgggat
    22141 tagtcataat cacccaaacc aataggagta ctagaaatga agaaagtagc aatgatggca
    22201 ttggccgtgg tcttcgcgtc ggccatgagc tcgcaggcgt ggtggatttt cggcaaaaag
    22261 gctgccgccg atcagcccgc cgtggagcag caggttgacc agaaggcttg cgccgaaaag
    22321 gccaaggccg actgcgctgc caagaaggcc gattgcaagg ctcaaaaagc cgattgcaag
    22381 gccaagaagg cggactgcaa ggccaagggc gagtgcaagt gcaccaagga caatgccaac
    22441 tgcaagaacc cgaattgccc catgaagcag gcggatggca aggccccgaa ggctgattgc
    22501 aaagccaaga aggctgattg cgcggcgcag aaggccgact gcaaggccaa gcaggcggat
    22561 tgcgcggcca aaaaggccga aaagcaggcc gagtgcaagg gcaaaaaggc tgaaaagaaa
    22621 gccgacgctc cggtgaaaaa ggctgagcgc aaaggcaaga aggccgcgga aaaagcggct
    22681 gaggccgtcg aagccccggc ggcggtagaa gctgctgaat aagcattctt ccccaagccg
    22741 tcaggcatcc ccggtcggat ttttctggcc ggggagtttt tcaatatatt attggttgag
    22801 agcatgagca aagacatctc cagattggtg gacggctggc cctacgagcc gggcgaagta
    22861 cgcgcccggt ggatcgaggc cgatgacggc tcgcgcaagc tgcaattgcg gctggatctg
    22921 gggattttcc agatggagcc caccgggcgg ccggacggca cctgcccgcg cggcgccatc
    22981 tccctgcgcg atttctacat cgaagaggaa atgcgttcgc ccgacgaaaa gctcccctac
    23041 aaactggaca acgacgcctg cgccgaactg caattggagg ccatgcagta ttattatcgc
    23101 atcatggcgt tttatgccct cggcgaacac aaaagcgtcg tcaaagattg cgagcacaat
    23161 ctcgatatta ttgacatcgt gagcgattac gccgccgacg acgaaaccgc ctggcagttc
    23221 aaccagttgt ttccctatat gcgcatgatg ctggcgcggg cggtggcgga agtggccatg
    23281 tcccgcgacc gtttcgaccg cgcggcccgc gcggtgaaag atggcatccg ccaaattgaa
    23341 caattttttg cggaaaacta tgagccgcaa aatgccgatg gcactccggc cccgcccccg
    23401 gcggaagtaa tctcgctgcg cgagttgctg gccgatatcg agcgccgccg ccccaagagc
    23461 gaggccgaaa atctgcgcgc cgaactggcc cgcgccgtgg aactggaaaa ctacgaaaaa
    23521 gccgccttcc tgcgcgatca actcaagcgt ttgcaggggc tggccacccg cccgcccgac
    23581 aaaaaacgca gccgctcgga gagcaaggag aaccggagtt gaacgcccgg ctggccatcg
    23641 gatgggcgct ggcgattggc ctcgtgattc cggtggcgca tggcgcgcct gccctttatc
    23701 agggcgaggg gagttgggga gccattacgg gcgcgtcggc cgacatcgcg ccgccagccc
    23761 tcggctgggt ctttcaagat ggcgcatggt tgggccgttt gcccgccaac gccgattccc
    23821 tgtccgtcgg tgccgaacgg ctggccatcg ctgatgcccg cctcgcgccg ccgcccaccg
    23881 tcgtactgcc gaaagaacgc aacgcctttg gcccttgcgc cgtgcggtcg ctatccatgc
    23941 gctatgatga agccgctcgc tcccggcgct ggaccattat tttttccgac gatcccgcgc
    24001 tggagccggc attttttttg ccgtcgctgg ttgtccgccc agtgctggag ctcgaagaca
    24061 tccagaccgg ccaacgctgg cccttgcagc cgacccgccg cggccgggaa ggggggcgtt
    24121 tccccacgcg ccatgatccc cgatattacg gtggaactct ggatgacgac gacattgaat
    24181 ggagcatgat ggtcattccg ctcgaaaacg ggcgacgcat tctgcagggc cggatcatgc
    24241 tgcaaaaaag cgatacccgc ttgttccgtc tgcgcgtggg cttggtgacc ggcacgccgg
    24301 gcaaggcggt gctgcaagat gagaacccgc ccgccgtggt gagcgtcagc gacggccaag
    24361 ctgtcgggct gttcccggat ttggctgaac cgcgccgctc acgcgtcgtg gccattccgg
    24421 ggcaggggcc cgccatcgag atggacttgg ccgtcaccaa ggccaccggt aattttccgc
    24481 gccgggtcac cttctcgttt gaggcggaaa cttggcccag cacggatgtc gccgccgcga
    24541 cgcaggaggc cgtggccaaa ctggctcgca cgggcggcgc ggtgccggta cccgatgccg
    24601 tcttgcgggc cggccccgat gcgctgccgg cctatgatct tatctgccat cgcctgacct
    24661 ttcccggtgg cttcgccaac gcacaggatg ctttccagtt tctccaactg aatatgtcga
    24721 gcttgtttgc cgattccgcc gattccgcca gcgcttatct ctccgtggcg catacggccg
    24781 cgggcgagcc gctagttgag ctgattcccg acggcgcgca agtcgccgtg aacccggacc
    24841 cggatttgga agccctgctg gagttagggc agaatcgcgg acaaacgctg ttggccagca
    24901 tcaaccgcca acgggctggc gcggtatgga ttcgcctcgg gccgtttgcc gatgaccttg
    24961 attgcagcgc ccgcggtctt tatatggccg actatcccgc cgcctggccc gctggcgtcg
    25021 ccaagccggg cgtggtgctg ggccacgcag ccgcggaatg ggtgtcttcg ctgtcctgcc
    25081 tattgcgcaa ggccaacacc tgcctgctgg tggaagacac cagcacgctt gcgccctaca
    25141 ccacagtgta tgccgacgcg ctcgtgtgca ccagtgcgga tcccgctgaa atgcgtcgtc
    25201 aacatgcgtt ggccggtccg cgccccgtcc tttgggccgc ccgccaatcc acggccgccg
    25261 ccaatgccct cgcgcacgat ttagggtttg tcacgtggcg acaaataaac gataactagt
    25321 tcatgctcga aaaccccgtt ggccgattac tcagtggcgc ggccgccacc ttggaaaaaa
    25381 gtgcgcgcgc ttggcaacgc caagccgtgg attgggatat gctggccgaa gccccgcccc
    25441 cgttgccgcc ccggatgcag accaccactg aacgcgaagt caccgtgcgc ggccgcggca
    25501 cgtttttcgg caagagcatc cgcaccctca ccctttgccc caccgacatg gaaggctggt
    25561 ggttcgagcg taccgatttg cccgattgcc tccccgtccg ctgctccatc cgcaacgtct
    25621 ggaccaccgg caacgtcgtc agcaacatcg tgctgcgcag cggctccccc cacaactata
    25681 tccgcatggc cgaacacatg attgccctgc gcatggggct cggcattgat aatctgctca
    25741 tcaaaattga ttccggcgac ccgccgctgt ttgatcgcgg cagcacggag ctggtgcaag
    25801 ccctgcgcag cgccggttct cgctccacaa ctcgctcgca accgctggtg accgtgcgcg
    25861 aacccgtcac ggtggtcagc ggcagcggca gtttcctcac gctggccccc gccgatccgg
    25921 cctgcccccg gctgaacatt gatgccgccg tggacttcaa gaccgccatc ggcaaacagc
    25981 gcatccgttt tgcggtggac ccggagcggt ttgcctttgg cgcgaccgcc cgcaccaatt
    26041 cttccgcgct caaaatgctc tattgccgca ccatcggcct gctttttgcc gacatccgca
    26101 acctcggcta ttcgctggac aacctgctgg tcgccgggcg cttctgctat tggaacaagc
    26161 ccctgctcaa gcataacggc aaatcgctgg aagccgtctg gcaccgcgcc acgcttgatc
    26221 tgctggcggc catcgccttg attgacgaag ggctttttgt cggcgacatc atctcctaca
    26281 aatccgggca tggccttgat gtggaaatgg tgcaacgcct ctatcaggag ggcttgctgc
    26341 agccgctggc tgctgcaaaa tagggcaggg gagatgaagc cggccgcgat gatccttctg
    26401 ttgctgggac tcgcgaccat ggttcacacc gctgccgggc aagccacgct gcccacgtca
    26461 tacaccggcc cctggtatct cgcattcccg cctgacggct ggacgttcag tgggctgggg
    26521 ggcccggact acatccccgg ctacgatccc gcccaagacg gcgcagccaa gttcaccagc
    26581 acgaacagcg ccatcaccat ccactttgcg cagccccccg ccgcgctcag ctattggatt
    26641 tgcggcaata tgtacaccgg cggcatcttc cgggtggagc aatccgtgga tggcacggct
    26701 tggaccacgc tgcaagacta caccgcattg cccaacgcct cgaaccacgt cacccacgcg
    26761 gtggacccca ccgcgcgtta tatccgtttc cattacctgg acaaattcac cggcaacgtt
    26821 ggggtggatg gcatcgccat tacccggttg gaatccctga ttccccggtt ttctcggttc
    26881 gtgtacgaaa acggcacgat caccgctcat ctggatgtgt cgttcgccga gtgtggttat
    26941 gtccttgaat ccacccccgg gctgaccacc aacactctgg attggtattt cgaggacgaa
    27001 cgctatggca ccgatggccc gctcgtcatg ccggccaacc cataccattc cggacccacc
    27061 cggttctacc gcgtccgcca agtctgccca taaaaaaatg cccgccggga atgcaaccct
    27121 ggcctcaaat acgacgtcag gatttgatgt aggccgccgt tgtatcggcg gcgggatcgg
    27181 aaaaaccaag caggaaaaaa gccggaggta cttggttctt ggtgctttgt gcttgggctg
    27241 ggaatgaaga tgaatctcgc agagatgcaa agcacgcgaa ggagagagat ggaaaggtgt
    27301 tggccattcc ggccgacaaa gacgcaacaa tccagacaat gatttttcct gctttcctgg
    27361 gttttttgtt ttcctgttcg gctgtcatgg ctgtttctgt agccgcgggc ggtgaccgcg
    27421 gtaggagtgg tcagggttca gaggtcaggt caaaaccgtc aaaccaagca gcccaacaat
    27481 ccaaccggct gcttttgtag ggcgggaatg gttgaagaat gaaaccttcc cgccgggcat
    27541 ggctggaagg tctgcgaccg ttccagccca gcaatccaac cggaaacaac tagagtgcgc
    27601 gaagttcaaa aaaaacgccc gccggcaagc ggcgggcgca ggattctctc aatggcgttt
    27661 accaactctt ccggtcggcg ctctcgttca cgaacacaat gtaccgatcc gtcgaggcga
    27721 accccagttt cacgcgggtt tccggataat aggtgtaatc cacatcgcgg atgcccgtca
    27781 tctttttgct ggctcccacc ggcaggataa aatcgcccca ttccgtggac aaggaacgca
    27841 ccgtgacctc ataggtggaa gcgttgacga actccagcgg ctgcttgtcg taatcctcgc
    27901 accccgtcag gcccaacatc aacaccgcca agcccaacat catcaacagt tttttcatga
    27961 acgacctcct gccatggtcc ggcaacctgc ccaaaactat gcgccagcgc cccggcccgg
    28021 tcaagcgaaa ctcgccacaa ccccatcttt tctcacgccg cgccgctgtc gtggtgaaag
    28081 caaaccttca cactttatgt agttagcaaa gccgcactt
//
LOCUS       DAOPNP010000016        27389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2241_length_27389_cov_7.184056, whole genome shotgun sequence.
ACCESSION   DAOPNP010000016 DAOPNP010000000
VERSION     DAOPNP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27389
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_27389_cov_7.184056"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKI64_02645"
     CDS             complement(<1..198)
                     /locus_tag="PKI64_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99938.1"
                     /translation="MWKIMGIVFHSVEKVAKRFPCRGKIGGLFSTVWKKSFHSVENGS
                     IRAGLSSTMGHPKLFMGADQVV"
     gene            complement(232..1044)
                     /locus_tag="PKI64_02650"
     CDS             complement(232..1044)
                     /locus_tag="PKI64_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD99939.1"
                     /translation="MIEVRDLRKTFFAGTANEVRALRSVSLDIPEGCFVAVIGTNGSG
                     KSTLLNAIAGTFAPDAGAVRIDGRDITRWSESRRARLIGRVFQNPFAGTAAGMTVAEN
                     LAIAMRRGLSRGLGFALPRRVRDEMIERVRTLQMGLETRLDNPIGTLSGGQRQALTLL
                     MATWLKPRLLLLDEHTAALDPKSAAQVAELTRQIIRRDKLTALMVTHSMQQAVELPDV
                     IVMMHGGQIVRQVAGPERERVRVPDLLAAFDHVRRREQLDEGVAGVLRAQYR"
     gene            complement(1037..1996)
                     /locus_tag="PKI64_02655"
     CDS             complement(1037..1996)
                     /locus_tag="PKI64_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD99940.1"
                     /translation="MTLVMGAFTIGLILSLLTIGVWIAFRVLRFTDITVDGSLTLGAA
                     VAAVLLVRGTPPWAATLAGAAAGGVAGFTTGMLHTRFKIQELLSGILVMTALYSINLR
                     VMGRSNVSLLSTKSIADPANRLLTAWAGGDSLLLLGWRVPVSEAAMLLTVLVIMVAIG
                     LLLRVFLVTHFGTALRAAGDNAQMIRAQGVDNRNMQVFGLCCANALVGLAGALLAQYQ
                     GFADVQMGIGMIVWGLASVIIGEALVSRVTPGLALAGAVMGTILFRLLIAIALRWGLN
                     PNDLKLVTALFVFAALVLPGQAKAWFGRARLPAGDRQGGRRHD"
     gene            complement(1993..3234)
                     /locus_tag="PKI64_02660"
     CDS             complement(1993..3234)
                     /locus_tag="PKI64_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD99941.1"
                     /translation="MQIELSQQGGWAVLRLEGRLDATWSEHVAARAREVLRSGRHHLL
                     LDAAGVSFLSSAGIRILIQLRRDVADVQGRFLVARPSEFVEKTLQLAGLESLLATEEL
                     LASVEAAGATPAGEGDGMALEVHRLDEAGEMTLNIAACWRPWDTVDAGDIRKISLPRG
                     TVALGIGAPDEDTTQAAGRMGEFMAVAGCMVHQPSDGQLHPPDYIAQRGGLVPELQAV
                     QALVAEGRFSHLLRFHPAAESARMDLAGLATAGLKAAGADAIVLVVLAECDGLVGARL
                     ARSPVAISNQEPPGDFPNVRDWIHFCGERLHAGKSALVVALVARSGGAGHLPAYLGAM
                     PSAPGLLVHAHAAAFTFRPLPAGIILAEEQIPALFEARDPLDLLHLLEDDRPLTGLGR
                     SQFVRGAAWCAPLRTPGEGGA"
     gene            complement(3224..5269)
                     /locus_tag="PKI64_02665"
     CDS             complement(3224..5269)
                     /locus_tag="PKI64_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD99942.1"
                     /translation="MNTIRQLWLGLVLIVAASMVLLVSDWGRREKNARTAATTVGVEK
                     AVPERLVVTGDVSALDFAEAKRAGMEYPLATLLQADRYLNVRELRGRLARVALVNLIY
                     ARSLELGEAGVRRGLLEAGLVEGEDFTLRKFNAQGDIAQLPALLDAAANWHPDVLVTV
                     TTPAMIAAVNRIKDIPVVFTIASSPIALGIFTKETVPANLTGVHDEPALGDLLDMARR
                     HVPGLKSVGIAYDASQANSVLTVTQLRAECAKRNLVLHEVTVSSMTDLGPAVQSLVQR
                     GMGALIVSTDNLINSGFAVVAKTAAQAGVPIFTTDADFIEEGATGAIGDNYELWGVQS
                     GELVAKVLAGVPVSALPLGPTRTHRVIPPKGAEGGPPLVPPTVSRPYEIRVIRYNDAA
                     FAEETSRGVEQGFIAADWQPGRHFRLKTYNAQGDMTTLNSIMASCRADAPDVVIPIST
                     PALQGALRQAGELPIVYGCVASGVQAGAGQSAVEHLPNVTGVDTLAPYDAMARLIRQM
                     LPGARAVGTLYSPGEVNAEYNREVFMAALKQEGLRLVSVPIQGTAATAESVNTLLHAD
                     IQVICQIVDNAARPGFAHIAKRATQAGLPFFCFDPSALPEGVALAMGRNYYDSGRETA
                     ELAMRVLRGESPGGIPFVRTDSRELVVNEAALNQFGLALPAEWREGAKVWTGEPHAN"
     gene            complement(5266..5964)
                     /locus_tag="PKI64_02670"
     CDS             complement(5266..5964)
                     /locus_tag="PKI64_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOD99943.1"
                     /translation="METARQGGAKVRGNAASGSKNARLLPPHGGYKNLLSYQKAEVVY
                     DATVRFCEDFFQKRDRTVDQMVQAARSGKQNTVEGSMASGTSREMELKLTNVARASLE
                     ELLTDYRDFLRSRGAVEWEKNSKEALYVRRLAAGKIAPPGGRGTTGTEGTKGRVVTYE
                     DFRCFEDRPAEVRANIVISLIKQTNYLLDQQIRKLEEIFLQEGGLRERMTRARLAARD
                     NPSPLTTSRHGGRP"
     gene            6221..6568
                     /locus_tag="PKI64_02675"
     CDS             6221..6568
                     /locus_tag="PKI64_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD99944.1"
                     /translation="MQLTHDTQNGIAILALAGRLDTRGAPELESHGRALFRDGARQLL
                     VDMAGVDYISSAGLRVLLVLAKEAAACGGKLALCCLTPAVNEVMTNSGFDTIIPLAAD
                     QQSALNTLQNPTA"
     gene            6586..7614
                     /locus_tag="PKI64_02680"
     CDS             6586..7614
                     /locus_tag="PKI64_02680"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD99945.1"
                     /translation="MNPPSIKTKPAPPACRLGLLFAFAFLSAFSASSQNDSPAAAPLT
                     SVRIALQWLPQSQFAGFYMARDHGFYRDAGLDVTLIHTGPGPSSLDFLSRGDADFATT
                     FLSDAIVAAEQFELVNVAQLSQRSSLMLVGLKEMGIHQPADLDGKPVSYWQTAFSASF
                     EAFFRQQHVRPVAVPQHYSINLFLRRGVAACAAMQYNEYHRIYQAGIDYDEVTVFRMG
                     DYGLGFPEDGIYSTAGFARQHPEVCRALRRATLAGWEYARAHPDEVVDTVLRESKQAD
                     VPANRPHTRWMLTHLLASIFPPDDPAPTGALKPHDYQAAARALKKAGLISRIQPFARF
                     APLEGDSP"
     gene            7611..9965
                     /locus_tag="PKI64_02685"
     CDS             7611..9965
                     /locus_tag="PKI64_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD99946.1"
                     /translation="MIRCGTIRARIMTWVLAVTSLALIAVIVWSYVTSRHRLEAEMEA
                     KARFLADGAARQIDAQLGTLQGIVHGLALALETRGFNLSFAEARALQDECVAGNPGIY
                     GCCIALEDSAAPPGWDDHAPWAFRNGGSLAYEGLSGASRAHRREDWYALPQVLGKPVW
                     SEPYSWNGVLMATYSVPLYTGTGTNRTFAGVVTCDLTLDWLEQMIARLPLGKDGYGML
                     ISRNATYVSHPHGDFVLNETFFSVAEERNDPELRAIGRRMVATDSGLQLFRSIVTHDV
                     SWLAHSSLRSGDWIMVAVISHDEMKASILRLARQQAGIGIAGLLVLVLATTLISRSIS
                     RPVSRLASAAETLSAGDLDAVLPAPRGNDEVSHLTRAFNRMRDSLRRHIADLRETTAA
                     RERMHSELRIARDIQMGLIPKTFPPFPDRTDLDLHAVLEPAREVGGDFYDFFLLDSNR
                     IVLAIGDVSGKGVPAALFMAVTRSFLRSAFRAETDPAAALTRINHDLIEGNDSCMFVT
                     LFCAVLDLGTGELRYANAGHNPPVIRQPDGRIEWIEQPHGPIAGVTADARYTTGTHSL
                     PADAALVLYTDGVTEAMNPGGNLYGETRLADHLAQQPLAADCRTTTDSLLRSIHQFAD
                     GAEQSDDITLLLIRRRQPADAPPTDEMCLTITNTLADQQRAMDELDTFLDAHPVPPKQ
                     QYAIRLALEELLTNVVKYAYTDNVPHPIHIHLRLATPPTLTITDDGQPFNPLQDAPPP
                     TLDGPAEDRPIGGLGLHLIQSLGMTLHYRRENSRNILTVLFPPA"
     gene            complement(10005..11243)
                     /locus_tag="PKI64_02690"
     CDS             complement(10005..11243)
                     /locus_tag="PKI64_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD99947.1"
                     /translation="MLRSVTKILIASTLLGLSASVVFGDVFDFGERPLGTEENRRLAA
                     YLAEQAQNMGYAAESLPFTCVRWERGASFLERGGVRVPLYPSPFSPAFDQTLDAAVAS
                     TTQQLKRVRGDGRLVFLRDELAKEPLTPRDYPFYFSALHREIYSLVDERRAAAIVAVT
                     GRHPMYDESPFPLFNDANFKTPSGYISQESYAALLDAPRSKIRTVIKSKVYPETGEQW
                     IFRKAAATTPAPGKILIFAHMDTAYNTPGALDNAAGVATLLLVMARLRDYAGPYDLEF
                     VPFNGEDSPMARGEVTYLDRYRKEMPDIRLGINLDALGFRGAKVAVSTYNLDAVDQEY
                     LDQQLSPFANLERGPEWVESDHSIFVFGGVPCLALTSADLANTVMTVAHTPRDTSANV
                     DRALLAEAANFIAQLVTALK"
     gene            complement(11251..11418)
                     /locus_tag="PKI64_02695"
     CDS             complement(11251..11418)
                     /locus_tag="PKI64_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99948.1"
                     /translation="MTLAGWIIMVLACGGLTGLLLWCVRRVVRTADAPEHLHAPLGTD
                     THDHPPRGTKC"
     gene            complement(11415..12995)
                     /locus_tag="PKI64_02700"
     CDS             complement(11415..12995)
                     /locus_tag="PKI64_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOD99949.1"
                     /translation="MAKRKRERWGSQLGVILAVTGSAVGLGNFLRFPGQAAKYGGGAF
                     LIPYLVAFLIVGLPMAWAEWALGRYGGACGHNSSPGIFRVLLGRKGSYAGILGTLMPL
                     MILTYYMFVEAWCLAYAWDYLTGVFARLGTDLGAIENHFMSFVGAGENGSLFKTAVGA
                     RTLFFFGLCFLANMVLIYRGISRGIERFCNWAMPLLVLCAVVVLVRVLTLPANPLHPE
                     QTVINGLGYLWNPVKPGETLWSVLSRPEVWLAATGQIFFSLSVGFGLILTYASYLRPE
                     DDVALSGLTACAGNEFCEVVLGGMIAIPAAFVFLGPAVVQDPPGTFGMGFVTLPNVFN
                     HMPLGSMFGFLFFFLLFLAAITSSLSMMQPSIALLEEGLNIGRKASVLILTSILMLGG
                     LFVTYFSKGFTALDTLDFWMANFFIFIFATFQSIVFAWGLGIRRGMEELERGAHIRLP
                     RILPFVLKYVSPVYLLAVFALWAWRNLPERWNAIIHPPADGPPVVLLSVLFILGVGAF
                     FVWVVSRANRIWDKEEIS"
     gene            13107..14090
                     /locus_tag="PKI64_02705"
     CDS             13107..14090
                     /locus_tag="PKI64_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOD99950.1"
                     /translation="MSETSSHLERARAVFDIEIAALQQTRNRLDGGFTAAVTLLQATL
                     ANGGKIIVTGVGKSFHIGQKIAATFTSTGAPANVLHPSEAMHGDLGLLQPADVVLALS
                     YSGESDELVNLLPIIKRTGVRIIALTGVLDATLARHSDVVLDGSVTSEACPFNLVPTA
                     STTVALAIGDALAIALLEARGMTREKYAQFHPGGAIGRALLLKVDDIMRTGARLAAVP
                     HTASVRDAVIAMTKAQAGAVAVVDENRHVVGLFTDGDLRRYLAGGATDVQSPVANLMT
                     HRPSTVNAGRLAADALAIFQQHHFDDLVVVHDDGTLAGMIDIQDLPKFKIL"
     gene            14167..15240
                     /locus_tag="PKI64_02710"
     CDS             14167..15240
                     /locus_tag="PKI64_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOD99951.1"
                     /translation="MKKPAFLKQLMMLRVLYALVPVTLAAIYLFGWRILALVAVSCLA
                     AFVVEWIMVPAKPGKISQAVFVTGALLGLSLPPTTPYWIAAVGAAFGILFGKMVFGGF
                     GKNIFNPAVVGRAFIFVCFPVEMTGGFVPAFRGLPGGFAQWSWETVKQLPDDLVAKGL
                     QVADGLTAATPMWGWRDYQLETSLWDLFCGQIGGAFEYLGHFRLLAAGSAGEVSALAI
                     LLGAIYLLWTKTANWRLMAGSLIGAVAMNVLLRNVLHITSVPPLLFSLLSGAFLFATV
                     FMVTDPISAPRIPLAMWIYAIFIGAMIIFIRWKAVFAGGVGFAILLGNMIAPSLDLWI
                     KRYRDSKKAAAPATPATVKGPAA"
     gene            15237..15926
                     /locus_tag="PKI64_02715"
     CDS             15237..15926
                     /locus_tag="PKI64_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOD99952.1"
                     /translation="MNKKSPAYVLAFMAAICLVFGAAIAVVNYATLGLLAKNEALHRN
                     TVWCRAFLLPVAGNAAEDYQRAVDENLVPLTLQDAAGEIKGYRRITPGHEAVGFLVGG
                     LGFWDRIEAAIVLTPDLREVINLQFMDQKETPGLGARVTEEWFTDQFKGRIVDWDGPA
                     DARLIVGPAPNPQAANRVDGITGATQTSMALARFLNSELNRIRALLPAALATAAAAPA
                     TAGESTLPQEP"
     gene            15941..16549
                     /gene="rsxE"
                     /locus_tag="PKI64_02720"
     CDS             15941..16549
                     /gene="rsxE"
                     /locus_tag="PKI64_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOD99953.1"
                     /translation="MASSYGKILKDGLVTENPIFRQVLGICSALAVTNLVTNTLYMGF
                     GVTFTTALTSFTIALLRNYIPVRIRMIVQVIIIAVYVMVVEILIKALAPDVHRFIGPY
                     VGLIITNCIIMGRAEAFASQNKPLPSLVDGLANGLGYTSVLVAIAVIREIMSFGTVLG
                     YKLPALDLWWHPWTIMVMPPGAFFMLAILTWMARARLVKEVK"
     gene            16549..17154
                     /locus_tag="PKI64_02725"
     CDS             16549..17154
                     /locus_tag="PKI64_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOD99954.1"
                     /translation="MELSQMSLFASCVLIFVSATFTDNILMARFLGMCSCLGVSKKVD
                     TSLGLGLAVMFVTAVTAALNYLVYQYMLVPLHLEYLKLIIFIVVIAAFTQLVEMIIER
                     TSEKLYNSLGIFLPLITVNCAILGISLFMLNKPYSFWQAFAFGLGGGLGWFLAIALIG
                     GIREKINESALPKGLEGPGITLIIIGIMALGFVGFSGMIKI"
     gene            17227..18327
                     /locus_tag="PKI64_02730"
     CDS             17227..18327
                     /locus_tag="PKI64_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOD99955.1"
                     /translation="MLTSILISVGFLLACGLILAALLVLAEKKILNYGTCHIHINNGK
                     KTLDVEGGSSLLNSLAEQNIFIPSACGGRGTCAYCKLKVNEGGGSISPVEEPYLSPEE
                     RQEHVRLSCQVKVRRDLSIEIPDDLFSVRRFHAKVARKRALTHDIVELRLALVEPTTI
                     EFEAGQYIQLESAEYKGHEAVMRGYSISSVPSDKTHLELIIRLVPDGICSTWVVQHLK
                     EGANITLSGPYGHFHLSDTTAPIICIAGGSGMAPIWSILRDMKEKGLNRPATYFFGAL
                     TQRDLFFLDEFKQLAKECPWFTFVPALSKEPADSSWQGERGLITEVVDRHFPDTSQHE
                     AYLCGSPGMIDAAIAVLKKNGMPEANIFYDKF"
     gene            18348..18911
                     /locus_tag="PKI64_02735"
     CDS             18348..18911
                     /locus_tag="PKI64_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99956.1"
                     /translation="MTTFHGSNDHANPPPADDSVPAMYTDQTAHPAPVHPKQAPEAKA
                     LEERLGASRFSGEGFLGTDQRTVDQIIAADVRTIEQLGITKAALLDALRAAFEKARAA
                     FGGEVEIRPGLTAVAHESMGRIPSPFRGDGVFEKGDVVLTSATGEELVLTALGLALIE
                     KHDFFQGHGSRYRIDPARAAHLLGILA"
     gene            complement(19637..21442)
                     /locus_tag="PKI64_02740"
     CDS             complement(19637..21442)
                     /locus_tag="PKI64_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99957.1"
                     /translation="MSAERQEQRPSRAESRRLMRWLLGYSWPYRWSMLGILLLTVVTG
                     LTMNYPPVLIRNAVDRWLAVSGISVDARLLGLLRTATLYFAISAGSLLLRYAQTLVTA
                     WLGQRIIRDIRRDVFDKALKLPLRFYDRTPVGRLLTRVTSDVDSVQSFVSDGVVGAIA
                     DVFQMVGVFVFMLVISPVLLAALVSIIPFLVVTFLFSHTRVRAANREIRRAQSALNTQ
                     AQEAISGMSTIQLFGREQTIRSRFSRLNRLMRDACFLEVRWFSFYFPVLELAQAISTV
                     LVLGVGGFCVLHGWGGVTLGVVIAFLTYVRDFFRPLDGLSNRAGMLQQALAACERIYQ
                     LFQEEEEISDPLQPALLPPTPQNIAFERVDFAYLADTPVLYEVSFTIAPGEIVAVVGA
                     TGSGKTTLVNLLMRFYAPTAGGIRLGAHDLRDLRQEELRRHFGTVRQEPFIFSATVAE
                     NIALMNPATSREDIIAAAQQANADAFIRRLEQGYDTKLRERGAGLSAGQKQLLALARI
                     MLQREARVLILDEATANVDSETEGWIQDALKTVMVGRTGIIIAHRLSTIRHADRILVF
                     QQGRLVAQGRHEDLLRQGGYYRRLYEYLAMTENSG"
     gene            complement(21439..23124)
                     /locus_tag="PKI64_02745"
     CDS             complement(21439..23124)
                     /locus_tag="PKI64_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD99958.1"
                     /translation="MIALGTGISLLTVGFNIAIPFVSRYVIDGLAARELGRGDLLRWL
                     GLYVVATAFATALSYWMRWLPLRVGHQVETDIRCDLFQHLTGLEREFFREHHTGDLLN
                     RLSSDVRAVGFGFGQGLMQNTRMIFVLFLGFGVMFSLNVKLAALVLALVPAMVGSFTW
                     FSQRIKRGHLLVQEELSEMASFNQESFAGIQTVKSFALEDRWRAQFAERNRALMQKNL
                     RLVRVHELTRPLAGFWFAVSSVLVLLVGGRLVIAEELSLGALVQFTQYLLYMQWPLLS
                     VGWVLNLIQRGWASWGRIDSLLQRAPGITDPPEVHAGENSAALGITFDRVGLKVGDTV
                     LLEDINLIIAPGMRVGITGPTGSGKTTLLSLLPRLMDVTSGAVRVGGRDVREWPLAEL
                     RGRIGMAEQEPVLFSDTLANNIRFGAPEVSDEAMLKAADLAHLHADVAQLPQQFDTLM
                     GERGVTLSGGQRQRTSLGRALSRDPRILILDDVLAAVDTETEAAILAKLRGVFASRTT
                     LLASHRVRTLAEMDWIVVLENGRITQRGTHQELIQQPGYYRRLHERQQLAAAI"
     gene            23474..24670
                     /locus_tag="PKI64_02750"
     CDS             23474..24670
                     /locus_tag="PKI64_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOD99959.1"
                     /translation="MKMLNIICGNLAKLTTMGRFSSHGAKRVRGAGSITKMIGVLTAP
                     NIGARWGQSKPSSKASLWSLAAIFAAMMLVSGCSQRPKHPLLLGHTMGTTFSVRIAHF
                     SLNLEQKRQLLGEIQGVLDEINRQMSVWDENSEISRFNKAPNGEFIEISLDFQAVVRR
                     ALEIAAATGGAFDPTVGPLVNLWGFGPKNGPQRGKPTAEQLAAVQASIGWQNVEINAD
                     GRLGKRIPGLQLDFGAIAKGYAVDRVTNLLLERGFSDFLVEIGGETRAFGQNPEYQAW
                     KIGILRPDGTQNIQNTVFLTNGRAIATSGDYRNFYRAENGEIETHIMDPRTGEPTKHK
                     TASVSVLADDCMTADALATALFVLGPDEGLPLLAEKFPGVEALFILREDDGSLREVAS
                     PNFFSR"
     gene            complement(24916..25752)
                     /locus_tag="PKI64_02755"
     CDS             complement(24916..25752)
                     /locus_tag="PKI64_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD99960.1"
                     /translation="MWCAGCASFPPRGGHSAWVPLHNAAAEGNLVGTMQLLQRGVDVN
                     ARDPQGYTALIAAAINGRLSVSRWLLLQGADVNAASADGMNALMGAAFTGNPDVIRLL
                     VEKGADINAQTPGDGATALLLAVWNNQTAAVAVLAELGADLNQRNAIGRSALDYAMQR
                     DAVPMVEQLLALGADPDARNAQGMTPLMVAAAQNQVEILKVLLNHGAEVNAQTAKGAT
                     ALMGAAFNGHLDAVRVLVDAKADVAHKNQAGLTALAAAERRGHQDVVDFLAKPKGKGK
                     SK"
     gene            25981..26691
                     /locus_tag="PKI64_02760"
     CDS             25981..26691
                     /locus_tag="PKI64_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOD99961.1"
                     /translation="MKLFDQEGGRMKRIFAILAGLMTMVLLSGCATTYAKRTSGSPLI
                     RSGAGVDLEEVRPERMLIWKANLSIEVWNISNAVNQASSMAERAGGFIEQKSDRGEES
                     SSLTLRIPAKAFKTALLDLETIGTVTYRNVTGEDITEDYIDVDARLKNKIVLRDRLKQ
                     LLEKATEVKDILSIETELNRVQADIDSMEGRIKSLKGQVDYATIRLSLNRKTILGPLG
                     YLFKGLWWGVEKLFVIRE"
BASE COUNT         5494 a         9564 c         7233 g         5098 t
ORIGIN      
        1 tacgacttga tctgccccca taaatagctt cgggtgtccc attgttgaac tcaggcctgc
       61 ccggattgat ccgttttcca cactgtggaa actttttttc cacactgtgg aaaaaagacc
      121 gccgattttt ccacggcatg gaaatctttt cgcaactttt tccacgctat ggaaaactat
      181 tcccatgatt ttccacactg tggaaacttt ttttccacgc tgtggaaaac tctaccggta
      241 ctgggcgcgc agcacaccgg cgacgccttc gtcgagctgt tcgcggcggc gaacgtggtc
      301 gaaggctgcc agcaggtccg gcactcggac tcgctcgcgt tcgggtcccg ccacctgccg
      361 gacgatctgt ccgccgtgca tcataacaat gacgtccggc agttcgaccg cctgctgcat
      421 cgagtgcgtc accattagcg ccgtgagctt gtcccgacgg atgatctgtc gcgtcagctc
      481 cgccacctgc gccgcgctct tcgggtccag cgccgccgta tgctcgtcta gcagcagcag
      541 gcgtggcttg agccaggtcg ccatcagcag ggtgagcgcc tgccgctgac cgcccgacag
      601 cgtgccgatg ggattatcga gccgcgtctc cagccccatc tgcagcgtgc gcacccgctc
      661 aatcatctcg tcgcgcacgc ggcgcggcag cgcgaaaccc aagccgcgcg acagcccgcg
      721 gcgcatggcg atggccagat tctccgccac cgtcattccc gccgccgtgc cggcaaacgg
      781 attctggaag acccgcccga tcagccgcgc ccgccggctc tcggaccagc gcgtgatgtc
      841 gcgcccgtca atccgcaccg cccccgcatc cggcgcaaac gtgccggcga tggcattgag
      901 cagcgtgctc ttgcccgagc cgttggtgcc gatgaccgcc acaaagcagc cctccggaat
      961 atccagactc acgctgcgca gcgcgcgcac ctcgttggcg gtgccggcaa aaaaggtttt
     1021 gcggaggtcg cggacttcaa tcatggcggc gccccccttg ccggtcgccc gcaggtaacc
     1081 gcgcgcgccc gaaccacgcc ttcgcctgac ccggcagcac cagcgccgca aacacgaata
     1141 gggcagtgac gagcttgagg tcgttgggat tcaggcccca gcgcagcgcg atggcgatca
     1201 gcagccggaa caaaattgtc cccatcacgg caccggccag cgcgaggccg ggtgtcactc
     1261 ggctgaccag cgcctcgcca ataatcacgc tggccagccc ccacacaatc atgccgatgc
     1321 ccatctgcac gtcggcgaat ccctgatact gtgccaatag cgcccccgcg aggccaacca
     1381 gcgcgttcgc gcaacacaag ccgaacacct gcatgttgcg gttgtccacg ccctgcgccc
     1441 ggatcatctg cgcgttgtcg cccgcggccc gcagcgccgt gccgaaatgc gtcaccagaa
     1501 acacccgcag cagcaggccg atggccacca tgatgaccag cactgtcagc agcatggcgg
     1561 cttcgctcac cggcacgcgc cagcccaaca gcagcaggct gtcacccccc gcccaggccg
     1621 tcagcagccg gttggcggga tccgcgatgc tcttcgtgct gagcagcgac acgttgctgc
     1681 gccccatcac ccgcaggttg atcgagtaga gcgcggtcat caccagaatc cccgagagca
     1741 gctcctgaat cttgaaccgc gtgtgcagca tccccgtggt gaaccccgcc acgccgcccg
     1801 ccgccgcgcc cgccagcgtt gccgcccagg gcggcgtgcc ccgcaccagc agaacggcgg
     1861 ccaccgccgc ccccagcgtc agcgagccgt ccaccgtaat gtcggtgaac cgcagcacgc
     1921 gaaacgcaat ccagacgcca atcgtcagca gcgacaaaat cagcccgatc gtgaaggctc
     1981 ccatcaccag cgtcatgccc cgccctcccc tggcgtccgc aacggcgcgc accaggcggc
     2041 gccgcgcaca aactggctgc ggccgaggcc cgtcagggga cggtcatcct cgagcagatg
     2101 cagcaagtcg agcgggtcgc gggcttcgaa caacgccggg atttgctcct ccgccagtat
     2161 gatgcctgcc ggcagcggcc ggaacgtgaa ggccgccgca tgcgcgtgaa caagcagccc
     2221 gggcgccgat ggcatcgctc ccaaatacgc cggcaagtga cccgcaccgc cgcttctggc
     2281 caccaatgcc accaccagcg cgcttttgcc ggcatgcagc cgctcgccgc aaaaatgaat
     2341 ccagtcgcgg acgttcggga aatcgccggg cggttcttgg ttggaaattg caaccgggct
     2401 ccgcgccaac cgcgcgccca ccagcccgtc gcactccgcc agcaccacga gtacgatggc
     2461 atccgcaccc gccgccttca atccagcagt ggccagcccc gccaaatcca tccgcgccga
     2521 ttccgccgcc ggatggaaac gcagcaggtg ggaaaaacgc ccctcggcga ccagcgcctg
     2581 taccgcctgg agctccggca ccagcccgcc ccgctgcgcg atatagtccg gcggatgcaa
     2641 ttgtccgtcc gatggctgat gcaccatgca gcccgccaca gccatgaact cgcccatgcg
     2701 gcccgctgcc tgcgtggtat cctcgtccgg tgcgccaatt cccaaggcca ccgtgccgcg
     2761 cggcagcgat attttacgga tgtccccggc gtccaccgtg tcccagggcc gccagcacgc
     2821 cgcgatgttc agcgtcatct cccccgcttc atccagccgg tgcacctcca gcgccatgcc
     2881 gtcgccttct cccgccggcg tggctccggc agcctccacg gatgccagca gttcctccgt
     2941 cgccagcaac gactccagac cggcgagctg cagcgtcttc tctacaaatt ccgatggccg
     3001 cgccaccaga aagcgcccct gtacgtccgc cacatcgcgg cgcaactgga tcaaaatgcg
     3061 gatgcccgcc gaactcaaaa acgatacgcc cgccgcatcc agcagcaggt gatgccgccc
     3121 gctccgcagc acctcccgcg cccgtgccgc gacatgctcc gaccacgtgg cgtccagccg
     3181 cccctcgagg cgtagcaccg cccagccgcc ctgttgcgac agctcaattt gcatggggct
     3241 ctcctgtcca caccttcgcg ccctcccgcc attccgccgg cagcgccaat ccaaattgat
     3301 tcagcgccgc ctcattcacc accagctccc gcgaatccgt ccgcacaaac ggaatgccgc
     3361 ccggactctc gccgcgcagt acgcgcatgg ccagttccgc cgtctcccgg cctgaatcgt
     3421 aatagttgcg ccccatcgcc agcgcgacgc cctccggcag tgccgaggga tcaaagcaga
     3481 aaaacggcag cccagcctgc gtcgcccgtt tcgcaatgtg cgcgaaaccc ggacgcgccg
     3541 cgttgtccac gatttggcaa atcacctgga tgtcggcgtg caacagcgta ttcaccgact
     3601 ccgccgttgc cgccgtcccc tgaatcggca ccgacaccaa ccgcaggcct tcctgcttca
     3661 gcgccgccat gaacacctcg cggttgtatt ccgcgttgac ctcccccgga ctgtagagcg
     3721 tccccactgc gcgcgcgccg ggcagcatct ggcggatcag ccgcgccata gcgtcgtagg
     3781 gcgccagcgt gtccactccc gtcacattcg gcaaatgctc aaccgccgat tggccggcgc
     3841 cagcctgcac gcccgacgcc acgcagccat acacaatcgg caattcgccc gcctggcgca
     3901 gtgcgccttg cagcgcgggc gttgaaatgg gaatcaccac gtccggcgca tccgcccggc
     3961 aggacgccat gatgctgttc agcgtcgtca tgtcaccttg ggcgttatag gttttcaaac
     4021 ggaaatgccg ccccggctgc cagtctgccg cgataaagcc ctgctccacc ccgcgcgacg
     4081 tttcctcggc aaaggcggca tcgttgtagc ggatcacccg gatctcatac ggccgcgaga
     4141 ccgtcggcgg caccaacggc ggcccccctt ccgcgccctt cggtggaatc acccggtgcg
     4201 tccgtgtcgg gcccagcggc agcgccgaca ccggcacgcc cgccaacacc ttcgccacca
     4261 gctcgccgga ctgaacgccc cacagctcgt agttgtcgcc aatggcgccc gtcgccccct
     4321 cctcgatgaa gtcggcatcc gtggtaaaaa taggcacccc cgcctgcgca gccgtcttgg
     4381 ccaccaccgc aaaaccggaa ttgatcaagt tgtcggtgga cacaatcaac gcacccatac
     4441 cgcgctgcac cagcgactgc accgccgggc ccaaatccgt catgctcgac actgtcacct
     4501 cgtgcagcac cagattccgt ttggcgcact cggcgcgcag ttgagtcacc gtcagcactg
     4561 aattggcctg cgacgcatca taagcaatcc ccaccgactt cagccccggc acatgccgcc
     4621 gcgccatgtc cagcaaatcc cccagcgccg gctcatcatg cacgcccgtc agattcgccg
     4681 ggactgtttc cttcgtaaaa atccccagcg caatcgggct gctggcgatg gtgaacacaa
     4741 cgggaatatc cttgatgcgg ttgacggcgg cgatcatggc cggcgtggtc accgtcacca
     4801 gcacatccgg atgccaattt gctgccgcgt ccagcagcgc tggcaactgg gcgatgtctc
     4861 cctgcgcatt gaacttgcgt agcgtaaaat cctccccctc caccagaccc gcctcgagca
     4921 gtccgcgccg cacccccgcc tcgcccaact ccaacgaccg cgcataaatc agattcacca
     4981 gcgccacccg cgccaaccgc ccgcgcagct cccgcacgtt caaatagcgg tccgcttgca
     5041 acaacgtcgc cagcggatac tccattcccg cccgcttcgc ctcggcaaaa tccagcgccg
     5101 acacatcccc ggtcacgacc aggcgctccg gcacagcctt ctccacgccg accgtcgtag
     5161 ccgccgtcct cgcattcttc tcccgccgtc cccaatccga caccagcaaa accatcgatg
     5221 ccgccacaat cagaaccaaa cccagccaaa gttgccggat ggtattcaag gacgcccccc
     5281 atgcctggat gtcgttaaag gagacgggtt gtcccgcgcc gcaagccgcg cccgcgtcat
     5341 ccgttcacgc agcccacctt cctgcaaaaa gatttcttcc agcttccgaa tctgctgatc
     5401 caacagatag ttggtctgct tgatcaaact gatcacaata ttcgcccgca cctccgccgg
     5461 acgatcctca aaacaccgaa aatcctcata ggtcaccaca cggcccttcg tcccctccgt
     5521 ccccgtcgtc ccccgccccc ccggcggcgc aattttcccc gccgccaacc gccgcacata
     5581 cagcgcctct ttgctgtttt tctcccactc caccgctccc cggctgcgca aaaaatcgcg
     5641 gtagtccgtc aacagctcct ccaggctcgc gcgcgccaca ttcgtcagtt tcaactccat
     5701 ttcgcgcgag gtaccggacg ccatgctgcc ctcgacagta ttctgcttcc cgctgcgcgc
     5761 agcctgaacc atctggtcca ccgtgcgatc tcgcttctga aaaaagtctt cgcaaaaccg
     5821 tacggtggca tcatagacca cttccgcctt ttggtaggac agcaggttct tgtatccccc
     5881 gtgcggcggc aacaagcgcg cgttcttgct gcccgatgcg gcgttgcccc gcactttcgc
     5941 gccgccctgt cttgctgttt ccatgcatag ccattgtgca atttcccgtt ccgacagagc
     6001 aagggcaaac caaccttttt cataaatcca ggggacccag gcgaccaagg cgacataggc
     6061 gccgccccat cacctatgtc ccctatgtcc tctatatcct ctttgtcccc agccccaatc
     6121 cccatccata attgctttct gacgcgggtt gtctacaatg gaaactgaat gagaacagca
     6181 cccaatctac tatgctggcg aaaaacccaa ggagaatccc gtgcaactga cgcatgacac
     6241 ccaaaacgga atcgccatcc ttgcgctggc cggccgcctc gatacccggg gcgcccccga
     6301 gttggaatcc cacggacgcg ccctcttccg cgatggcgcc cgccaactgc tggtggatat
     6361 ggcgggcgtg gactatatca gcagcgccgg cctgcgcgtg ctgctcgttc tcgccaaaga
     6421 ggccgcagcc tgtggcggca agttggccct atgctgcctc acccctgcgg tcaacgaagt
     6481 catgaccaac tccgggtttg acaccattat tccgctcgcg gccgaccagc aatccgcgct
     6541 caatactctg caaaacccga ccgcctgaca cacggagtcg cacgggtgaa cccgccctca
     6601 attaaaacca agccggcacc ccccgcctgc cgcctcggcc tcctgttcgc ctttgcgttt
     6661 ctctccgcct tttccgcctc gtcgcaaaac gacagcccag ccgccgcccc gctcacctct
     6721 gtacgcatcg ctctccagtg gctgccccaa agccagttcg ccggattcta catggcccgc
     6781 gaccacggct tctaccgcga tgccgggctc gatgtcacgc tcatccacac cggccccggc
     6841 ccgtcctcgc tggactttct ttcccggggt gacgcggact tcgccaccac atttctcagc
     6901 gatgccatcg tggccgctga gcaattcgag ctcgtcaacg ttgcacagct ctcgcagcgc
     6961 tcctccctca tgctggtcgg cctcaaggaa atgggcatcc accagcctgc cgatctcgac
     7021 ggcaagcccg tgagctattg gcagaccgct ttctcggcct ccttcgaagc cttctttcgg
     7081 cagcagcacg tccggcctgt tgcggttccc cagcactatt ccatcaacct ctttctgcgc
     7141 cgcggggtcg ccgcttgcgc cgccatgcaa tacaacgaat accaccgcat ctatcaggcc
     7201 ggcattgatt acgacgaggt caccgtcttc cgcatgggcg actacggcct cggcttcccc
     7261 gaagacggca tctattccac cgccggtttc gcccggcagc accccgaagt ctgccgagcc
     7321 ctccgccgag ccacgctcgc cggctgggaa tacgcccgcg cccatcccga tgaagtcgtg
     7381 gacaccgtcc tgcgtgaatc caaacaggcg gatgtccccg ccaaccggcc ccacacccgc
     7441 tggatgctga cccacctgct cgcctccatc ttcccgcccg atgaccccgc ccccaccggc
     7501 gccctcaaac cgcacgatta ccaggccgcc gcccgcgcct tgaagaaagc cggtctcatt
     7561 tcccgcattc agccattcgc ccgctttgcc cccctcgaag gagactcccc atgatccgct
     7621 gcggcaccat tcgcgcgcgc atcatgactt gggtgcttgc cgtcaccagc cttgcgctca
     7681 tcgccgtcat cgtctggagc tatgtcacct cccgccatcg cctcgaagcc gaaatggagg
     7741 ccaaagcccg ctttctcgcc gatggcgccg cccgccaaat tgacgcccag ctcggcaccc
     7801 tgcaggggat tgtccacggc ctcgccctcg cgctggaaac ccgcggattc aatctctcct
     7861 tcgccgaggc ccgtgccctg caggacgaat gcgttgccgg caatcccggc atctacggct
     7921 gctgcatcgc cctcgaagac tccgccgccc cccccggctg ggatgaccac gcgccgtggg
     7981 cattccgcaa cggcggctcg ctcgcctacg aaggcctcag cggcgccagc cgcgcccacc
     8041 gccgcgaaga ttggtacgcc cttccccaag ttctcggcaa gcccgtctgg agcgagccct
     8101 actcatggaa tggcgtcctc atggcaacct attccgtccc gctctacacc ggcaccggca
     8161 ccaaccgtac ctttgccggc gtggtcacct gcgatctcac cctcgactgg ctcgagcaaa
     8221 tgatcgcccg cctccctctc ggcaaggacg gctacggaat gctcatcagc cgcaacgcca
     8281 cctatgtttc ccacccccat ggcgatttcg tcctcaatga aactttcttc agcgtggccg
     8341 aagaacgcaa cgatcccgaa ctgcgtgcca tcggccgccg catggtcgcc accgattccg
     8401 gtctccagct ctttcgcagc atcgtcaccc acgatgtctc gtggctcgcc cattcttccc
     8461 tgcgttccgg tgactggatc atggtggccg tcatctccca cgatgaaatg aaagcctcca
     8521 tcctgcgcct tgcccgccag caggccggca tcggcatcgc cggcctcctc gtgcttgtcc
     8581 tcgccaccac cctcatctcg cgatccatct ctcgccccgt cagccgcctc gccagcgccg
     8641 ccgaaaccct ctctgccggc gacctcgacg ccgttctccc cgctccccgc ggcaacgacg
     8701 aggtatccca cctcacccgc gccttcaacc gcatgcgcga cagcctccgc cggcacatcg
     8761 ccgatctccg cgagaccacc gccgcccgcg aacgcatgca cagcgagctg cgcattgcac
     8821 gcgacatcca gatgggcctt atccccaaaa ccttccctcc cttccccgac cgcaccgacc
     8881 tcgaccttca cgccgtcctc gaaccggccc gcgaagtcgg cggcgacttc tacgatttct
     8941 tcctcctcga ttccaaccgc atcgtcctgg ccattggcga tgtctccggc aaaggggtcc
     9001 ccgccgccct cttcatggcc gtcacccgca gcttcctgcg ctccgccttc cgcgccgaaa
     9061 ccgatcccgc cgccgccctc acccgcatca accacgatct catcgaaggc aacgactcct
     9121 gcatgttcgt caccctcttc tgcgccgtgc tcgatctcgg caccggcgaa ctccgctacg
     9181 ccaacgccgg ccacaatccg cccgtcatcc ggcaacccga cggccggatc gaatggatcg
     9241 aacagcccca cggccccatc gccggcgtca ccgcggacgc ccgctacacc accggcaccc
     9301 acagcctccc cgccgacgcc gccctcgtcc tctacaccga cggcgtcacc gaagccatga
     9361 atcccggtgg caacctctat ggcgagaccc gcctcgccga ccacctcgcc cagcagcctc
     9421 tcgccgccga ctgccgcaca accaccgact ccctcctccg tagcatccac cagttcgccg
     9481 acggtgccga acaatccgac gacatcaccc tcctcctcat ccgccgccgc caacccgccg
     9541 atgccccccc caccgacgaa atgtgcctca ccatcaccaa caccctcgct gaccagcagc
     9601 gcgccatgga cgagctcgac accttcctcg acgcccaccc cgtaccgccc aaacagcaat
     9661 acgccatccg cctcgctctc gaagaactcc tcaccaacgt cgtcaaatac gcctacaccg
     9721 acaacgtccc ccatcccatc cacatccacc tccgactcgc caccccgcct accctcacca
     9781 tcaccgacga cggccaaccc ttcaaccccc tccaggacgc ccccccaccc acgctcgacg
     9841 gccccgccga agaccgcccc atcggcggcc tcggcctcca cctcatccaa tccctcggca
     9901 tgaccctcca ctaccgccgc gaaaactcgc gcaacattct caccgtcctc ttcccccccg
     9961 cctgatcccg ccccgcccaa ccatttgggg tcgcctattc aggcttactt cagcgcagtc
    10021 accagttggg cgatgaagtt ggcggcttcg gccagcaagg cgcggtccac gttggcgctg
    10081 gtgtcgcgcg gggtgtgggc aaccgtcatc accgtgttgg ccaaatcggc cgaggtgagg
    10141 gcaaggcaag gcacgccgcc gaaaacaaaa atggaatgat cgctttccac ccactccggg
    10201 ccgcgttcca gattggcaaa cggcgacaac tgttgatcga gatactcttg atccaccgca
    10261 tccagattat aggtcgaaac ggccactttg gcgccgcgaa aacccagagc atccaagttg
    10321 atccccaggc gaatatcagg catttctttc cgatagcgat ccagataggt cacctcgcca
    10381 cgcgccatgg gtgaatcttc gccattgaac ggcacgaact ccagatcgta agggccggca
    10441 tagtcgcgca ggcgcgccat gaccagcagc agcgtagcga cgccggccgc gttgtccaac
    10501 gcgccgggcg tgttataagc cgtgtccata tgggcaaaaa tcagaatctt gcccggcgcc
    10561 ggggtagtcg cagccgcctt gcggaaaatc cattgctcgc cggtttccgg atagaccttg
    10621 gacttgatga ccgtgcggat tttgctgcgc ggggcatcca gcaatgcggc gtaactttcc
    10681 tgagaaatat aaccgctggg cgttttgaag ttggcatcgt tgaacagcgg gaacggcgac
    10741 tcatcataca tggggtgccg gcccgtcacg gcaacgatgg ccgcggcccg ccgctcatcc
    10801 accaaggaat aaatttcccg gtggagcgcc gaaaaataga atgggtaatc acgcggagtc
    10861 agcggctcct tggccagctc atcgcgcaaa aagaccagcc gcccgtcgcc gcgcacccgt
    10921 ttgagttgct gggtggtgct tgccaccgcg gcatccagcg tttggtcgaa tgcgggcgaa
    10981 aacggactgg gatacagcgg cacgcgcacg ccaccgcgct ccagaaacga agcgcccctc
    11041 tcccaccgca cacaggtgaa cggcagcgat tccgccgcgt agcccatatt ttgggcttgc
    11101 tccgccaaat aagccgccag ccgtcggttt tcctccgtgc cgagtgggcg ctccccgaag
    11161 tcaaacacat caccaaatac cactgacgcc gacaggccaa gcagggtgga ggcaataaga
    11221 atcttggtta cggatctcag catttgctgc ctagcatttt gtgccgcgcg gcgggtggtc
    11281 gtgagtgtca gtgccgaggg gggcgtggag gtgttcgggg gcatcggcgg tgcgcacaac
    11341 gcgacgcacg caccacaaca gaaggccggt caagccgccg cacgccagca ccatgatgat
    11401 ccaaccggcc agagtcatga tatttcctcc ttatcccaaa tgcggttggc gcgcgacacc
    11461 acccaaacga agaaagcgcc cacgcccaag atgaacagca ccgagagcag caccacgggt
    11521 gggccatccg ccggcgggtg aatgatggcg ttccatcgct ccggcaggtt gcgccacgcc
    11581 cacagtgcga acaccgccag cagatagacg ggcgacacat acttcaacac aaacggcagt
    11641 atgcgcggca accggatgtg cgccccgcgc tccagctctt ccatcccgcg ccggataccc
    11701 aacccccatg caaacacgat ggattggaac gtggcaaaaa taaaaataaa aaagttggcc
    11761 atccagaagt ccagcgtatc gagcgcggtg aaccctttgg aaaaataggt cacgaacagg
    11821 ccgccaagca ttaggatgct tgtcaggatc aacaccgagg ctttgcgccc gatgtttaac
    11881 ccttcttcca gcagggcaat cgagggctgc atcatggaga gcgaactggt gatggccgcc
    11941 aaaaacaaca ggaagaaaaa gaggaagccg aacatcgaac ccagcggcat gtggttgaat
    12001 acgttgggca aggtgacaaa acccatgccg aaggtgcccg gcgggtcctg caccacggcc
    12061 ggccccaaga acacaaatgc cgccggaatg gcgatcatgc cgcccaagac cacctcgcaa
    12121 aattcattgc cggcgcaggc ggtcaggccg ctcagtgcca cgtcatcttc cgggcgcaga
    12181 taactggcat aggtcaggat caatccgaat cccaccgaca ggctgaagaa gatttgcccg
    12241 gtggccgcca gccacacttc ggggcgggac aacaccgacc acagcgtttc gccgggtttg
    12301 acggggttcc atagataacc caatccgttg atcacggttt gctccggatg cagcgggttg
    12361 gccggcaatg tcagcacgcg caccagcacc accaccgcgc agagcaccag caacggcatg
    12421 gcccagttac aaaaccgctc gataccgcgg ctgatccccc ggtaaatcag caccatgttc
    12481 gccaaaaaac acaatccaaa gaaaaacagc gtgcgcgcgc caaccgccgt tttgaacagg
    12541 ctgccgtttt cgcccgcgcc gacaaaactc atgaaatggt tttcaatggc ccccaagtcc
    12601 gtgcccaacc gcgcgaagac cccggtcaga taatcccacg cgtaggccag acaccacgcc
    12661 tcgacaaaca tgtagtaggt caggatcatc agcggcatga gggtgccgag aatcccggcg
    12721 taggaccctt tgcgccctag cagcacccgg aaaatccccg gcgaagaatt atgcccgcac
    12781 gcccccccgt agcgccccag ggcccattcc gcccacgcca tcggcaaacc cacgatcagg
    12841 aaagccacca aatacggaat caggaaggcg cccccgccat atttggcggc ctgcccggga
    12901 aagcgcaaaa agttgcccag ccctaccgcg ctgccggtca cggcgagaat cacgccgagc
    12961 tgacttcccc accgttcacg tttgcgtttg gccatgcgcg cgatctccaa tccttcgatg
    13021 taatatgccc cggcccgccg ccctctgtca aaactcgatc acgcggattc ttgctctccg
    13081 cccgcctttt aggctacctt gcccccatga gcgaaacctc ttcccatctg gaacgcgcgc
    13141 gagcggtgtt cgatatcgag atcgccgcgt tgcaacaaac ccgcaaccgg ctcgatggcg
    13201 ggttcaccgc cgcggtgacc ctgttgcaag ccacgctggc caacggcggc aaaattattg
    13261 tgactggcgt cggcaaatcg tttcatatcg gccagaaaat tgcggccacc ttcaccagca
    13321 ccggtgcgcc ggccaacgtg ctgcatccct ccgaggccat gcacggcgat ctcggcctgc
    13381 tgcagcccgc cgatgtggtg ctggcgcttt cctattccgg tgaatcagat gaactcgtca
    13441 atttgctgcc gattatcaaa cgcaccggcg tgcggattat cgccctgacc ggcgtactgg
    13501 atgccacgtt ggcccgccat tctgatgtgg tgctggatgg ctcagtgacc agcgaggctt
    13561 gcccgtttaa ccttgtgccc accgcttcca ccacggtggc gctggccatc ggcgatgccc
    13621 tcgccatcgc cctgctggag gcccgcggca tgacccgcga aaaatatgcc cagttccatc
    13681 ccggcggggc catcggccgc gcgctgctgc tcaaagtgga cgacatcatg cgcaccggcg
    13741 cgcgcttggc cgccgtgccc cacaccgcca gtgtacgcga tgccgtcatt gccatgacca
    13801 aggcgcaggc cggcgccgtg gccgtggtgg atgaaaaccg ccacgtggtg gggctgttca
    13861 ccgatggcga tttgcgccgc tacctcgccg gcggggccac cgacgtccaa agccccgtgg
    13921 ccaacctcat gacgcacaga ccatccacgg tgaatgccgg gcggctggcg gcggatgcct
    13981 tggccatttt ccagcagcac cactttgacg atttggtcgt ggttcatgat gacggaacac
    14041 ttgccggcat gattgacatt caagacctgc cgaagttcaa gattttgtga ttatgtgatt
    14101 tctcgccaat tgttatcgcg ccgcttgaaa tccaatttgc cctaggctac cttccccgca
    14161 attgccatga aaaaaccagc ttttctcaag cagctcatga tgctgcgtgt gttgtacgct
    14221 ttggtccccg tcactctggc tgccatctat cttttcggct ggcgcatcct ggctctggtg
    14281 gccgtttcct gtttggcggc gtttgttgtg gaatggatca tggtgcccgc gaagcccgga
    14341 aaaatctcac aggccgtgtt tgtcaccggt gcgttgctcg gcctctctct gcctcccacc
    14401 acgccgtact ggattgcggc cgtgggcgca gcgttcggca ttctcttcgg taaaatggtg
    14461 tttggtggtt tcggcaaaaa tattttcaat cccgccgtgg tggggcgcgc attcatcttt
    14521 gtgtgcttcc cggtcgaaat gactggtggg ttcgtgccgg ccttccgcgg cctccccggc
    14581 ggcttcgcgc aatggagttg ggaaacggtc aagcaactgc ccgatgatct cgtggcaaaa
    14641 ggattgcaag tggcggatgg tctcactgcc gccacgccca tgtggggctg gcgcgattat
    14701 cagttggaaa ccagcctctg ggaccttttc tgcggccaaa tcggcggcgc gtttgaatat
    14761 ctgggccact tccgcctgct tgcggccggc tccgcgggcg aagtttccgc gctggccatt
    14821 ctgctgggcg caatttacct gctttggacc aagaccgcca actggcgctt gatggccggc
    14881 tcgctcattg gcgcggtggc catgaatgtg ctgttgcgga acgtgctgca catcaccagc
    14941 gtgccgccgc tgctgttcag cctattgtcc ggcgcattcc tgttcgccac cgtgttcatg
    15001 gtcaccgacc cgatttccgc gccgcgcatc cctttggcca tgtggatcta cgccatcttc
    15061 atcggcgcca tgattatttt tatccgctgg aaagccgtct tcgccggggg cgttggcttc
    15121 gccatcttgt tgggcaacat gatcgcccct tcgctggacc tttggatcaa gcgctaccgc
    15181 gatagcaaaa aagccgccgc gccggccacc cccgccaccg tgaaagggcc cgccgcatga
    15241 ataaaaaatc gcctgcctat gtgttggctt tcatggcggc catctgcctc gttttcggcg
    15301 cggccatcgc cgtcgtcaat tacgccacgc tcggcctgct ggccaagaat gaggcccttc
    15361 accgcaacac ggtctggtgc cgcgcgttct tgctgccggt tgcgggcaat gccgcggagg
    15421 attatcagcg cgccgtggat gaaaatctgg tgccgctcac actccaagat gccgccggcg
    15481 aaatcaaagg ctatcgccgc atcactcccg gccatgaagc cgtggggttt ctggtcggcg
    15541 gcctcggttt ttgggatcgt atcgaagccg ccattgtcct taccccggat ttgcgcgaag
    15601 tgatcaacct gcaattcatg gatcaaaaag aaacccccgg cctcggcgcg cgcgtcacgg
    15661 aagaatggtt caccgaccaa ttcaaaggcc gcatcgtgga ttgggacggc ccggcggatg
    15721 cccgcctgat tgtcggcccc gcgcccaatc cgcaagccgc caaccgggtg gatggcatta
    15781 ctggcgccac gcaaacctcg atggccctcg cccgtttcct aaacagtgaa ctcaaccgga
    15841 ttcgcgccct gctgcctgct gccctggcca ctgccgccgc ggctcccgcc accgccgggg
    15901 aatccacctt gccacaagaa ccctgacccc cacccacgct atggcttcct cttacggcaa
    15961 aatcctcaaa gacggcctcg tcaccgagaa cccgattttc cgccaggtgc tgggcatctg
    16021 ctcggcgctg gccgtcacga atctggtgac caacaccctc tacatgggct ttggcgtcac
    16081 cttcaccacc gcgctcacca gcttcacgat tgccctgctg cgcaactata tcccggtgcg
    16141 catccgcatg attgtgcagg tgatcatcat cgcggtgtac gtcatggtgg tggaaatcct
    16201 gatcaaggcg ctcgcgcctg atgtccaccg ctttatcggc ccctatgtcg gcctgatcat
    16261 caccaactgc atcatcatgg ggcgcgcgga agccttcgcc agccagaata agccgctgcc
    16321 gtccctggtg gacgggctgg ccaacggtct cggctacacc tcggtgctgg tggcgattgc
    16381 cgtcatccgc gaaatcatga gcttcggcac cgtgctgggc tataagctgc ccgcccttga
    16441 cctctggtgg cacccgtgga ccatcatggt catgcccccc ggcgctttct tcatgctggc
    16501 cattctcacg tggatggccc gggcccgcct cgtcaaggag gtcaagtaat ggaactctcc
    16561 caaatgagcc tcttcgcttc gtgcgtgctg atttttgtat cggccacatt caccgataac
    16621 atcctcatgg cgcgttttct gggcatgtgt tcctgcctgg gtgtgtccaa aaaggtggat
    16681 accagtctcg gtctcggcct cgccgtgatg ttcgtcaccg cggtcaccgc cgcgctgaac
    16741 tatctggtgt atcaatacat gctcgtgccg ctgcacttgg agtatctcaa gctgatcatc
    16801 ttcattgtgg tcatcgccgc cttcacccaa ctggtggaaa tgatcatcga gcgcacctcg
    16861 gaaaaactct acaactcgct tggcattttc ctgccgctga tcaccgtcaa ctgcgccatc
    16921 ctcggcattt cgctgttcat gctgaacaag ccctacagct tctggcaggc gtttgcattc
    16981 ggcctcggcg gcggcttggg ctggttcctc gccattgccc tgatcggcgg catccgcgag
    17041 aaaatcaacg agtccgcgct gcccaagggg ctcgaaggcc ccggcatcac gctcattatc
    17101 atcggcatca tggccctcgg ctttgtcggc ttctccggca tgatcaaaat ctaaacccat
    17161 gaacacaccc acgctcaaca cacacgcgcc cgccgccctg ccgcggcgcc tgcttgcggg
    17221 atagccatgc tcacgtccat cctcatttcc gtcggattcc tcctcgcctg cggcctgatc
    17281 ctagccgcac tgctggtgct ggccgaaaag aaaatcctca actacggcac ctgtcacatc
    17341 cacatcaaca acggcaagaa gaccctcgat gtcgaaggcg gctcctcgct gctcaactcg
    17401 ctcgccgagc aaaacatctt tatcccctcc gcttgcggcg ggcgcggcac ctgcgcctat
    17461 tgcaagctca aggtcaacga aggcggtggc tccatcagcc ccgtggagga accctacctc
    17521 tcccccgagg agcggcagga gcacgtccgc ctctcctgcc aagtcaaagt gcggcgcgac
    17581 ctttccatcg aaatccccga tgacctcttc tccgtccgcc gcttccacgc caaggtcgcc
    17641 cgcaaacgcg ccctgaccca cgacatcgtc gagctgcgcc tggcactcgt cgaacccacc
    17701 accatcgagt tcgaagccgg ccaatacatc cagcttgaat ctgccgaata caagggccac
    17761 gaggccgtca tgcgcggcta ctccatttcc tccgtgccct ccgacaaaac tcatctggaa
    17821 ctaatcattc gcctcgttcc cgacggcatc tgctccacat gggtggtgca gcacctcaag
    17881 gaaggcgcca atatcaccct ctccggcccc tacggccact tccacctctc ggacaccacc
    17941 gcgcccatca tctgcatcgc cggcggctcc ggcatggctc ccatttggtc catcctgcgc
    18001 gacatgaaag aaaagggcct caaccgcccc gccacctatt tcttcggcgc ccttacccag
    18061 cgcgacctgt tcttcctcga tgaattcaag caactcgcca aagagtgccc ttggttcacc
    18121 tttgtccccg ccctctccaa ggagcctgcc gattcttcct ggcaaggcga gcgcggcctg
    18181 atcaccgagg tggttgaccg ccatttcccg gatacctccc agcacgaggc ctatctctgc
    18241 ggctctcccg gcatgattga cgccgccatc gccgtgctca agaaaaatgg catgcccgaa
    18301 gccaatattt tctacgataa gttctaacca actggaggag gcaagcgatg actacgtttc
    18361 acggctcaaa cgaccacgcc aatccccccc ccgccgatga ttccgtcccg gccatgtaca
    18421 ccgatcaaac ggcgcacccc gcccccgtcc atcccaagca agcgcctgaa gccaaagcct
    18481 tggaagaacg cttgggcgcc tcgcgcttct ccggcgaagg tttcctcggc accgatcagc
    18541 gcaccgtgga ccaaatcatc gccgccgatg tacgcaccat cgagcaactt ggcattacca
    18601 aggccgccct cctcgacgcc ctccgcgccg cgtttgaaaa ggcccgcgcc gcatttggcg
    18661 gcgaggtgga aatccgcccc ggcttgaccg ccgttgccca cgaatccatg ggccgcatcc
    18721 cctccccctt ccgcggcgat ggcgtttttg aaaaaggcga tgtcgttctg acctccgcca
    18781 ccggcgaaga actcgtgctg accgccctcg gcctcgcgtt gatcgaaaaa cacgactttt
    18841 tccaaggcca cggctcgcgc tatcgcattg atccggcccg cgctgcccac ctgctcggca
    18901 tcctcgcgta agccaccccc acccgcgtcg taccccgcgc actccccgtt aattgttggg
    18961 ccggaacggt cgcagacctt ccagccgctc ccggcgagaa gctttcatcc ctccaccatt
    19021 cccgcccaac aaaagcccca cgagaacacc cacggccgaa tccggtttgt aggcggggtt
    19081 gcacccccgg cgggctgtta tttccgggcc aaggattgac ggatcggttt gtagggctgg
    19141 aacggtcgaa gaccttccag ccgctcccag cgtcagccgc cgttttcggt gaaaggatcg
    19201 tctcgctggg agtgttgaaa ccgctgttgt cacgctgcct tcgtttttct catctgaacc
    19261 atgctgtcca gagtttataa actctgggca gatgtagtaa tatttttatc tcaatcatac
    19321 acaatatatt atgtctattt tgtaggctgt ttttaatcag ccattttgat ggcactgtcc
    19381 acactttata aactctggac aaattttggg tgagtgattt ttgcgttttt atccgcgcgg
    19441 ttcggtcccg catggaaggc gccgatccca aaatccccac cacaatcagc gaaattctgg
    19501 ggtgtcctaa tgcgaaattc aggaaggtag cctgacgcaa ctggccgagg aagtagctgc
    19561 gactcgtgga agtgtcgcgc acccggatcg gattgttggg ccggaacggt cgcagacctt
    19621 ccagccgtta ccggcgtcag ccgctgtttt cggtcatagc caagtattcg tacaggcgcc
    19681 ggtaatagcc accctgccgc agcaagtctt catgcctccc ttgggccacc aaacggcctt
    19741 gttgaaacac caaaatgcgg tcggcgtggc ggatcgtgga taaccggtgg gcaatgataa
    19801 tgcccgtgcg ccccaccatg accgtcttca gcgcatcctg aatccagccc tcggtttcgc
    19861 tatccacatt ggccgtggct tcgtccaaaa tcagcacgcg ggcctcgcgc tggagcatga
    19921 tgcgcgccaa cgccaagagt tgcttctgcc cggcggacag acccgcgccg cgctcgcgca
    19981 atttggtgtc atatccctgc tctaggcggc ggataaatgc gtctgcattg gcttgctggg
    20041 cggcggcgat gatgtcctca cgggatgtgg ccggattcat cagggcaatg ttctccgcca
    20101 cggtggccga aaaaataaat ggctcttggc gaacggtgcc gaaatgccgg cgcaattcct
    20161 cttgccggag gtcgcgcaga tcgtgggcgc ccaatctgat gccgcccgcc gtgggcgcgt
    20221 aaaagcgcat cagcagattc accagcgtgg ttttgccact gcctgtggcc cccaccaccg
    20281 ccacaatctc tcccggggcg atcgtgaatg acacttcata caataccggc gtgtcggcaa
    20341 gataggcgaa gtccacccgc tcaaacgcaa tgttttgtgg cgtgggcggc aacaaggccg
    20401 gttggagcgg gtcgctgatt tcttcttcct cctggaacaa ctgataaatc cgttcgcacg
    20461 cggctaaagc ctgttgcagc atccctgcgc ggttggagag cccgtccagt ggtcggaaaa
    20521 agtcccgcac ataggtcagg aacgcaatca ccacgcccag cgtcaccccg ccccagccat
    20581 gcagtacgca gaaaccaccg acacccagca ccagcacggt cgaaatggcc tgcgccagtt
    20641 ccagcacggg gaaatagaag ctgaaccagc gcacctcgag gaagcaggcg tcgcgcatta
    20701 gccgattcaa gcgtgaaaag cggctgcgga tggtttgctc gcggccaaac aactggatgg
    20761 tgctcatccc gctgatggcc tcttgcgcct gggtgttcag cgcggactgc gcccgccgaa
    20821 tttcccggtt ggcggcccgc acccgggtgt gcgaaaacag aaacgtcacc accaggaagg
    20881 gaataatgct caccagcgcc gccagcagca ccgggctgat caccagcatg aagacgaaaa
    20941 cgccgaccat ttggaacacg tcggcaatcg ccccgaccac accatccgag acaaaactct
    21001 ggacgctgtc cacgtcggat gtcacccgcg tcagcaaacg ccccaccggc gtgcggtcat
    21061 aaaaacgcaa cggcaacttc agcgccttgt cgaacacgtc ccggcggata tcgcggataa
    21121 tccgctggcc cagccaagcc gtgacgagtg tttgggcata gcgcagcaac aagctgccgg
    21181 cgctaatggc aaaatacagc gtggccgtgc gcagcaaacc cagcaagcgg gcgtccacgc
    21241 tgataccact cacggccagc cagcgatcca ccgcgttgcg gatgaggacc ggcggataat
    21301 tcatcgtcag cccggtcacc accgtcagca gcagaatccc caacatgctc cagcggtacg
    21361 gccacgaata gcccagcaac cagcgcatca ggcggcgcga ctccgcccgg ctgggccgtt
    21421 gttcctgccg ctccgcactc atatcgccgc cgccaactgt tgccgctcgt gcagccgccg
    21481 gtaatacccc ggctgctgga tcaattcttg gtgggtgccg cgctgggtaa tccggccatt
    21541 ttccaacacc acaatccaat ccatctcggc caatgtgcgc acccgatggc tcgccagcaa
    21601 tgtggtccgc gaggcaaata ccccgcgcaa tttggccaga atggccgcct cggtttccgt
    21661 gtccaccgcg gccagcacgt cgtccaagat gagaatgcgc ggatcccgcg acaacgcgcg
    21721 acccaacgac gtgcgctgcc gctggccacc ggaaagcgtc acgccgcgct cacccatcag
    21781 cgtgtcgaat tgttggggca actgagccac atccgcgtgc aaatgggcca agtcggcggc
    21841 ctttaacatg gcctcgtcgc tgacctccgg cgcgccgaag cggatattgt tggccaacgt
    21901 atcggaaaac agcaccggct cctgctccgc catgccgatt cggccgcgca attccgccag
    21961 cggccattcg cggacatcgc gcccccccac ccgcaccgcg ccgctcgtca catccatcaa
    22021 ccgcggtaac agcgagagca gagtcgtttt cccgctgccg gtcggcccgg tgatccccac
    22081 ccgcatcccc ggcgcaatga tcaggttgat atcctcaagc aaaaccgtat cgccgacttt
    22141 taggcccacg cggtcaaacg tgatgcccaa cgccgcgcta ttttctcccg cgtggacttc
    22201 tggcgggtcc gtgatgcccg gggcgcgttg caacaggctg tcaatgcggc cccaactggc
    22261 ccacccgcgt tgaatcagat tcaacaccca ccccacggaa agcaacggcc actgcatata
    22321 cagcaggtat tgggtgaact gcaccaaggc gccgagcgaa agttcttcgg caatcaccag
    22381 ccgcccaccc accaacagca ccagcacgga gctgaccgca aaccaaaacc ccgccagcgg
    22441 gcgggtcaat tcatgcaccc gcaccaaacg cagatttttc tgcatcagcg cgcggttgcg
    22501 ctcggcaaat tgcgcgcgcc agcggtcttc caacgcaaat gatttcaccg tctggatgcc
    22561 ggcaaagctt tcctgattga agctcgccat ttccgacagt tcttcctgca ccaacaaatg
    22621 gccgcgcttg atccgctgcg aaaaccacgt gaagctcccg accatggccg gcaccaacgc
    22681 cagcaccagc gccgccaatt tcacattcag gctgaacatc acgccaaacc ccaggaacag
    22741 cacaaaaatc atgcgcgtgt tctgcatcag cccttggccg aacccgaacc ccaccgcccg
    22801 cacatccgac gacagccgat tcagcaaatc gcccgtatgg tgctcgcgaa aaaactctcg
    22861 ctccagcccg gtcaggtgtt ggaacaaatc gcagcggata tccgtttcca cctgatgccc
    22921 cacccgcagc ggcagccaac gcatccagta cgaaagcgcc gtcgcgaatg ccgtagccac
    22981 cacatacagc cccagccagc gcagcaaatc gccccggccc agctccctcg ccgccagccc
    23041 gtcaatgaca taccgcgata caaacggaat cgcgatattg aacccgaccg tcagcagcga
    23101 aatccccgtc cctaacgcaa tcatgcgcca gtacgggaca atataccgcc ataaacgccg
    23161 gttggctgtc tttcctgcac ccataaaacc cacccacgat acaccccgcc gcaccaaacc
    23221 tcacgatcgt ttttaccccc gccctaaaca cctcaatcgg ttggttgatg ctaccttgga
    23281 tgccaaggta ccttggcacc caaggtagtc agcactgaca gcgagagaag ttgacgatgc
    23341 ggggtggtcg gaatctattt gaggcaggtg attcgtcggc agaacaccca aaacaatcct
    23401 tggagaaggg caaccagctt ggcaggatag atatggcgaa atggtgcagg cttgctgcgc
    23461 gcaagccgaa cgaatgaaaa tgctcaacat aatttgcggg aaccttgcca agctgacgac
    23521 gatgggccgc ttttcctctc atggtgccaa gcgcgtgcgt ggggcaggtt ctatcaccaa
    23581 aatgattggc gttttaaccg cgccaaacat tggcgcgagg tggggccaat caaagccgag
    23641 cagcaaggcc agtttgtgga gtttggcagc catttttgcg gccatgatgc tggtgagcgg
    23701 gtgttcccag agaccgaagc atccactttt gctcggacac acgatgggga cgacattttc
    23761 ggtgcgaatt gcccattttt cgctgaattt agagcaaaaa aggcaacttt taggcgaaat
    23821 tcagggcgtt ttggacgaaa ttaaccgcca aatgtcggtt tgggacgaaa atagcgaaat
    23881 ttctcgcttt aataaggccc caaacggtga attcattgaa atttcgctag attttcaggc
    23941 cgttgtgcgg cgcgcgctgg aaatcgcggc agcaaccggg ggggcatttg acccaacagt
    24001 tgggccattg gtcaatttgt ggggttttgg ccccaaaaat gggccgcagc gaggaaaacc
    24061 cacggccgag caactagcgg cagtgcaggc gtccattggc tggcaaaacg ttgaaatcaa
    24121 cgccgatggg cggctaggca agcggattcc gggcttacag cttgattttg gggccattgc
    24181 caaggggtat gcggtggatc gggtcacaaa tttgctgttg gaacgcggtt tctctgactt
    24241 tttggtcgaa attggcggcg aaacccgcgc ttttggccaa aatccggagt atcaggcctg
    24301 gaaaatcggc attttacggc ctgatggcac ccaaaacatc caaaatacgg tgtttttgac
    24361 caatgggcgg gccattgcga cttccggtga ttatcgcaac ttttaccggg ccgaaaatgg
    24421 cgaaatcgaa acgcacatca tggatccaag gaccggcgaa ccgacgaagc acaaaactgc
    24481 cagtgtgtcc gtattggcgg acgactgcat gactgccgat gccttggcca ccgcgttgtt
    24541 tgtcttgggg ccggatgaag gattgccgtt gctggcagaa aaatttccgg gcgtggaggc
    24601 gctatttatc ctgcgcgaag acgatggctc attgcgcgaa gtcgcaagcc ccaacttttt
    24661 ctctcgctga attgcaaaaa aatcaagccc ccccgctatt tttgcagggt tttgtagggc
    24721 gggaatggtt gaggaacgaa accttcccgc cgccataggc tggaaggtct gcgcccgttc
    24781 cagccctaca atccgacccg tcaatccttg acccggaaat aacagcccgc cggggatgca
    24841 accccagcct acaaaccgga ttcgcccccc ggccttgttt cataaattgg cggggttttg
    24901 gcgaatgaat acgttctatt tgctctttcc cttgcccttg ggcttggcca agaaatccac
    24961 cacatcctga tggccacggc gctcagcggc ggcaagggcc gtcagtcccg cctgattctt
    25021 atgggcgacg tcagccttgg cgtccaccaa gacccgcacg gcatcaagat ggccattgaa
    25081 cgccgcgccc atgagggctg tcgcgccttt tgcggtctgc gcattcacct ccgccccgtg
    25141 gttgagcagt accttgagaa tctccacttg gttttgcgcg gcagccacca taagcggggt
    25201 catgccctgc gcattgcggg cgtctgggtc cgcgccgagc gccagcaact gctcaaccat
    25261 cgggacggca tcgcgctgca tcgcgtaatc caacgcggaa cggccgattg cattccgttg
    25321 gttcaggtcc gcacccaatt cggccaatac cgccacggcg gctgtttggt tgttccaaac
    25381 cgccaagagt agcgcggtgg cgccatcgcc cggagtttgt gcgttgatat ccgccccctt
    25441 ctctacaagc aggcggataa catccggatt gcccgtaaat gccgcgccca tcaacgcatt
    25501 catgccgtcc gcgctggccg cgttgacgtc tgccccctgc aagagcaacc aacggctgac
    25561 gctcaatcgt ccgttgatgg ctgccgcaat cagcgccgta tagccctgcg gatcccgcgc
    25621 attgacatcc accccgcgtt gtaacagttg catagtgcca accaaattgc cttcggctgc
    25681 ggcgttgtgc aatggcaccc aggccgaatg accgccccgc ggcgggaagc tggcacaccc
    25741 cgcgcaccac atcatcccca ccagcaaaat tcccgtccat cgccgcatgc tcatcgtcct
    25801 ccgtcctgac cggcatccca gtgcatttag taacactggc acagttgcga acaaaagaaa
    25861 atcccgaatc ctacggcaac cacacggcat ttttagcttt tccaattgac cttgctgcag
    25921 aatgccaact aggatttcac cagatgattc gctgtggctt tagcaattcc gcattcgtcc
    25981 atgaaactgt tcgatcaaga aggaggaagg atgaaaagaa tattcgcaat ccttgccggt
    26041 ctaatgacga tggttcttct ctctggctgt gccactacct acgcgaagcg gacaagcggc
    26101 tcgccgctca tacgttctgg cgctggcgtc gatttagagg aagtgcgacc tgagagaatg
    26161 ttgatctgga aggcaaacct gagtatcgaa gtatggaaca tcagcaatgc agtaaaccaa
    26221 gccagttcaa tggccgaacg agccggcggg tttattgagc aaaaatccga tcgcggagag
    26281 gaatcctcca gcctgacact tcgtatccca gccaaggcat ttaagacggc gttgttggac
    26341 ttggagacga tcggcaccgt aacttaccgg aatgtgacgg gagaggatat cacggaagat
    26401 tacattgacg ttgatgccag gctgaagaac aagatcgtgc tacgcgacag actgaagcag
    26461 ctccttgaaa aagctacgga ggtcaaagat atcctgtcca tcgagacgga actgaacagg
    26521 gtgcaggccg acattgactc aatggagggc cggatcaagt ccctcaaggg gcaagtcgat
    26581 tacgcaacga ttagattgag tctgaacagg aaaaccattc tcgggccgct cggctactta
    26641 ttcaaaggac tgtggtgggg tgttgagaaa ctgtttgtga tccgggagta gggcggatcg
    26701 aacagggcgc tccaccgttc ggcgcacaag gcaccgccgg tgatcgctga ctttagcgcg
    26761 aaaaataatg agaacattga cgattccaat agttgcagtt ctcctcgctg gtctccttgg
    26821 cacagctcgc gcggaaataa accgtaacga tatcgtaaat cctgagattc ttgctccagc
    26881 ttcggtatcc caaggccaat ttgctgccct cctctgccaa tacctgaagc aagccacaaa
    26941 cgaggcgaca tacagagaat tgaaggatgc ctccgagcct gtggtctttg cgccacaaga
    27001 cgtggctgca gcagtcgagt atctatccga gatcggactg gttccctacg gcggatggca
    27061 accaactgaa gcgttgcgga ggcatcatat cacaagtctc atcgtacgag cctacggggc
    27121 ccatgacaaa ctggacattg gggatcaaga cgcttgcttc ggattcgtcg agtccaaagg
    27181 ctgcgacatt tccacaatca cgcgcgctat tgattgcatc tggaggcaga taagaagctc
    27241 caaccagggg cttgagggtt ctcccgactc gccagtccgc ggtgctttgc cgtaggattg
    27301 aactgcgcca aggttgtggc atactccccc ctgcgcttgg caccccatgc ccgggtggcg
    27361 aaatggcaga cgcaagggac ttaaaatcc
//
LOCUS       DAOPNP010000017        27202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2262_length_27202_cov_7.291008, whole genome shotgun sequence.
ACCESSION   DAOPNP010000017 DAOPNP010000000
VERSION     DAOPNP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27202
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2262_length_27202_cov_7.291008"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..795)
                     /locus_tag="PKI64_02765"
     CDS             complement(199..795)
                     /locus_tag="PKI64_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOD99962.1"
                     /translation="MTSSTFPDKLFNVTLVGVGGQGILLTSDILAMAAMHAGYDVKKS
                     EVHGMSQRGGSVISQVRFGTRVHSPIIPTGETDILVSFERLEALRWAHLARPGAKILV
                     NNMDRIPVTVSSGLQPAAQNVDERLAAYPGLILLAANELAVQAGNLRTANVVVMGALA
                     KLVPFGEDHWQAAMRERIPAQLLDVNLRAFALGYAAIA"
     gene            complement(806..2575)
                     /locus_tag="PKI64_02770"
     CDS             complement(806..2575)
                     /locus_tag="PKI64_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD99963.1"
                     /translation="MKKLLSANQAIAWAAYRAGCRVAAAYPGTPSTEIMEHIAEFAGA
                     INCEWSVNEKVAMEIGIGASLAGTRALVAMKHVGLNVAMDPLMTFTYIGAIGGMVIIN
                     ADDPSLHSSQNEQDNRSLAKFAKCLLFEPADSQEAYDMVCAGFELSEKYQVPVIVRTT
                     TRTAHTSTVVDIGSGERSETPVIPYVKNAQRNVAVPAHGRLMRFAVEKRSQALRALTE
                     ESPFNRADSGTGDIGFVASGVAYQYVKEVFPTAPVFKVGLSYPPPLEKIRAFAAGVQR
                     LVVIEESDPVLAEQIRAAGIPVVEPKTELHMMELDPVRLGALRAELLGTPPPPTAQPE
                     TGLPVRPPVLCSGCSHRGAFHILAKLKATVCGDIGCYTLGSAPPLSAMDTCVCMGAAI
                     GTAIGMRKAGLPNQRIAAVMGDSTFFHSGMTGLLDVAYNNIPITVMVFDNRITAMTGH
                     QENPGSGHTLLGVESPAVNIADIARTFGIQRVHEVEPYDLANLEKVFKECLDCGEPAV
                     VVVRGPCVLHVKWAGKYINTVDEVKCTACGSCFRIGCPTIVRGAPKDANGRLFKAKID
                     PQFCTGCNLCAQVCKFGAISPVK"
     gene            2741..3379
                     /locus_tag="PKI64_02775"
     CDS             2741..3379
                     /locus_tag="PKI64_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99964.1"
                     /translation="MKKLILFGCAALLAAGVSFGGVVSEDPGELSVVGQVFFPDSADC
                     DLFKIGYGAQVAYREWFSFPWGAGLTLGVSQWQVDSKSDAFKYDMLSNYDGDVLVIPI
                     GLALYFNVIDWDNWNLLLETGLQYAIVNSSATVFNNEPEAQKKEDVDIEGAILWNLGA
                     EFEYMVAENMYLQVAAGYQLDLMKGDTEYAGRSVRDASFAAGYARIGAKFLF"
     gene            3461..4201
                     /locus_tag="PKI64_02780"
     CDS             3461..4201
                     /locus_tag="PKI64_02780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOD99965.1"
                     /translation="MNRCFLPADRWGQPEAELSAEESHHLAGVLRAQAGQRIGLLDGA
                     GRTGEAEVVAPHKKHTRIRILSRQQAPANVPRRILAQALVREQQMDWLIQKAVELGVH
                     EIWPLQTDHAVVKVRAEDGGKKVARWQSVALAACKQSGNPWLPTIRPVQKLAGALAGL
                     PPAPAAACFGALQSGAMPLPAYLTGLRQAVCSTITMFIGPEGDFSQAEVAAMLAAGVQ
                     PVTFGPLIFRVETAALFILSAVHYAWMK"
     gene            4214..4741
                     /gene="tpx"
                     /locus_tag="PKI64_02785"
     CDS             4214..4741
                     /gene="tpx"
                     /locus_tag="PKI64_02785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOD99966.1"
                     /translation="MQELAPQPFTIQGEPAFTFTALPAIGSVAPDFRLADQHFRDWAL
                     ADFAGKKKILNIVPSLDTPICQLSAQKFNEAVAKRDDAVVLTISADLPFAQARFCANW
                     KTPNGQFLSTFRSPEFGMNYGTLIVEHALMGLQARAVIVLDEHNVVRHVQLVPELKQE
                     PDYTAALAALESVDA"
     gene            4738..6198
                     /locus_tag="PKI64_02790"
     CDS             4738..6198
                     /locus_tag="PKI64_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD99967.1"
                     /translation="MNTIVQSLAWGLSRLAGPVAYPPETDAPYAPGEAYPELAGQTRP
                     GGQPNPIFAAVREAMHKMGLDADHFGTPAWNPLGMLVKPGGNIVVKPNWVLHKNLGQG
                     GMECMITHPSVLRAVLEYVFLTKPALVVLGDAPLQGCDFERLLDYAGLRAVIAEFQNR
                     GLPLVIKDFRRTILREEGPLKKVAEELRAEDQYELVDLGADSLLEAISADWKKFRVTM
                     YDPRLMWRHHRPGRHRYLVAKEILNADLVVNVPKLKAHKKAGVTCCLKNLIGINGNKE
                     FLPHHRKGAVSAGKGDNYPNRHWLVTLAEHLLDVINCLRGWPRLYRQGERLVWWFLHF
                     AERKDATTQIEGSWHGNDTIWRTCLDLNRALLFADCAGQMTDTLQRQEISIVDAVITG
                     QGEGPLAPDPLETGAILAAHNPAIGDLVATRLLGFVPEKIALLRHACDDMKWRICAAP
                     PVVPEFTPPFPPARAPKGWAGHIEATGALVPPNSDS"
     gene            6195..7013
                     /locus_tag="PKI64_02795"
     CDS             6195..7013
                     /locus_tag="PKI64_02795"
                     /inference="COORDINATES: protein motif:HMM:NF020514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HOD99968.1"
                     /translation="MNVGRSLRWHNARVNGARKIANLLWRLRYNGNEFSIISNNCWGG
                     AIYQSLHRPYLSPFAGIFLMAPCYLRLLADLRHYLSRPPRQTTKSQYASVHRVYGPTL
                     PYPLGILDDNIEIHFLHYAAWEEALDKWQRRTERINWDRLFIKFGDQNDSSLGAVQQF
                     LQLSYERKLCLTNRQEVHGPAVVAMKGIGPTTRFQEDVEFRRYMDVPDWLGEGRARGG
                     AGLRRLSHWADGHYVQAYQERMAALLATGESLPMNLDAQLAAMDQSPPVRPPQL"
     gene            7016..8596
                     /locus_tag="PKI64_02800"
     CDS             7016..8596
                     /locus_tag="PKI64_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOD99969.1"
                     /translation="MSLRIKSFKQAAFWSTAINAFSQGLALLFSILMAKYFGAQESTD
                     ILYFCLGIFVLLGGLLQVVNVSVLIPETMRRRHQVSEQDAMAFINRFFAVFVLMIGAV
                     TVLSLSNPVLALSAISRFDIAAVERNMRLAQWLLLSFPLQMTAQLLLDILVSYKFLML
                     PALLSCVNRLINILFVVVFYRRFGVVSVAMGMVVGFSLQVALNAFLLRRKIHWKLSAW
                     RTRITRQTYWNILWVELGTLSGVLASYLPIYLFSGFAAGVVTILNYARRMSLMPVELL
                     TVQVSSVIAVKFNEQAAMRNFAGMGKSFGWLQRQQIFFLTPLAFLLGIAGYPIISLLY
                     GHGAFDANAVAQTARLFSVLMLMLPFLSVDNAVARLFVAHQEVALGTRYQVMGSILNA
                     VLIFIFVKWKGTMGFAVAMLLTRLLYLLILSAIFSKRFRPITLWPTTRSILLTFAYCG
                     VLACGALWLASSPFFVTLAASWRGVLAAGIFIAGYLASLVVCPPDRLARNEAITLGVE
                     LWARIRHIHSKWTETVPS"
     gene            8593..9660
                     /locus_tag="PKI64_02805"
     CDS             8593..9660
                     /locus_tag="PKI64_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99970.1"
                     /translation="MNQDLQIGIQKPAGLNFAERWAVALEKSGCRVRWLDLLGTDPLS
                     QIQGCDGIMWHWFHYPHEIRLAALPILRVIEEQLRIPVFPDMATAWHYDDKIAQSYLL
                     KALNIPHPQTWVFWRKADALRWAETAQYPVVAKLSGGAGSMNVRLLRSAKEARQYVNA
                     CFSGAGIVGRPALPRGALSATLARLKRALKRVAQAGPYVLANRFPALPDQTYWMPQKN
                     YALFQEFLPDNAHDTRVTVIGNRAFAFRRFNRPNDFRASGSGNLCVDPNGIDMRCIAA
                     AFDAAAKLKSQSMAFDYLFRGAEREPVVGEISYCYADWAVESCPGHWDSNLTWHAGRM
                     WPQEAHVEDFVARIVGARTLP"
     gene            9657..10910
                     /locus_tag="PKI64_02810"
     CDS             9657..10910
                     /locus_tag="PKI64_02810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD99971.1"
                     /translation="MKILMVVAHFMPVHRGGAEMQCWKQAKALAQRGHKVTVLTPWWS
                     LSSKRSEWVDGVRIRRMGFLLPLTWLARRWHARLKPPPAEGGGEVVSATPKARKKRFR
                     WMSLTERPGLFSFILEVACAIRFRRLRPDVIHVHESHWIAGFAHWMAERLDVPVFCKE
                     GLLPVLDFGGAQHVPWRERWRERRTKCRFIAMTDAIQDALIKAGIAPDQIACVPNGVE
                     IPERKAQPQLYGDALYVGNLSQGAAHKGFDVLLKAMGEVVKEAPEIKLRICGAGVATG
                     WQAMADELGCGTAVEFVGRVEDLTEYHHHSGFLVLPSRKEGISNALLEAQAAGLPAIV
                     TDIPGNTAVIQHGFNGLVVPVGDAVALARAIVEMHRSTELRERLGHAARQQAEARFAI
                     SRVAEQLEELYHTALVATTPDGFQD"
     gene            10951..12492
                     /locus_tag="PKI64_02815"
     CDS             10951..12492
                     /locus_tag="PKI64_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99972.1"
                     /translation="MSTAPSNFPTSQRTTYQFPDYRWIGWLTVAGFALGLTVMFFIAG
                     MFGIPAPLGWAFLVILFALGALLLDRPKLLLSFMIFYFLFMPSNRMLGLVSLPLPDFL
                     DELFFLPFIAVIAMNWIQRRQLTEATFFPVAFIGVAIMSSIVNDRPSVFTALQVTLIM
                     LKSYILWYFCRLTCTFSNTRQLMRWVWFYVIYVAFQFLYNILWQHGPWPHYHPDRSGG
                     VFGPDGNGAAHLVGYLSVFALMLLTGWWVSSAHRSTIRQRIAVVLLTLVIAYNLIFMT
                     DTKHALLMMPLAFIPFLLHPKFPVRLRVWLLLAGAVFGLATMVYFNVAVGQVNIRRYL
                     NGMRDSPKGEMFYAVTKDFRFLVRYPLLGAGPGCFGSPQSVDARTPLARRYIIPYRDE
                     IRRLDYFGKGLGGTRAAASVLGMPQSDLFTLMGEFGWLGALVYYSFLGWVVLRFWQKS
                     AALPLRHPLSGLYMACCCCVIFLSFVTVLVPAATIPLLVFPLWMLIARMWDMPGNEME
                     MADGT"
     gene            12482..13633
                     /locus_tag="PKI64_02820"
     CDS             12482..13633
                     /locus_tag="PKI64_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD99973.1"
                     /translation="MAPDGPPAETLYSAAPWANRTGAPICALEHLIALRDYFGPICLI
                     LPEHGPLEKLAAAANVSVWVSPLVYKGLRQGGFWRFWCGIGPVLRSRWQYLRRLIRQL
                     RSQPGILHIHSRSTHLPYALLAGRLARIPMVVTIHEPWAGGVEAWLDVLLIHWLADRV
                     ILVSQNMADTYPRFLCRRAVISYYFSSVPALSARPESRSPLRIGMLARMGHRKGADIF
                     LATCRLLRERGIPCEAWVAGGWNSEAEQKVAADYMAAHQLQSVVRDLGLVEDMPALYR
                     QLDVLFLPARRDPLPRVVMEAMCHGLPVVASRVDGIPEMVVDGETGILVEPENVEGFA
                     DALMRLLADPELRQRMGAAAHARAQKIFTVEAYRGAMLKAYKGLLPTKP"
     gene            13804..14766
                     /locus_tag="PKI64_02825"
     CDS             13804..14766
                     /locus_tag="PKI64_02825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD99974.1"
                     /translation="MNAQSPKVGIALLNWNQYDDTALCLESLRESKFRPAIILVYDNG
                     STDGSAERLKANFPEIQLVVGGQNFGFSEGNNRAVQNLLEAGMDLVWVLNNDTKVSPD
                     CLGWLVHSLDEQPEVGAVSGKIWFMEETRPLCYAGGIFNRWNFNTTFLGFREPDSGQY
                     DIGGDTTIFSGCCMLIRAEVIRKIGLFNRGFFAYAEDIDWSLRAQASGIRLYYEPRAT
                     LWHKMFGSTVKTKGGAKKSSEFVEFLSARNRFLLVRLHTRPWNVRRCFALSWHLWVRG
                     IPRAIGLMLLPSRRAVGWARLKGLWAGLWMHPDPANCMLNSPAS"
     gene            14940..15704
                     /locus_tag="PKI64_02830"
     CDS             14940..15704
                     /locus_tag="PKI64_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD99975.1"
                     /translation="MSSIPPTIHYCWFGGGPKPALAERCLASWRRYLPEHELREWNEG
                     SFDISNPPYVREAYRLKKYAFVADYVRLHALQTHGGIYLDTDVELLRNPDTVLTAPAV
                     LGFETPERVCSAFLAFAPGHELIHEWLETYRNRSFLRDGKPDETTNVQVLTKLLRSRG
                     LVLDGTEQRLRGGIVVYPQDVFSPYTYGERKPRTTARTVAIHWLEASWATGSTRAKLV
                     GIQAIKRLFGAGFYHWLRRIWSASRRRRANRGDRLA"
     gene            complement(15900..16640)
                     /locus_tag="PKI64_02835"
     CDS             complement(15900..16640)
                     /locus_tag="PKI64_02835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD99976.1"
                     /translation="MNIFQSIWGALTKPFRTLHSILAYQLQPPPLNEFTDYDNYWEKR
                     GKVPVIYRRWEVAAETFELNSTVLDVGCGNGEFLSYLREKRPDLILKGCDTSAIGVER
                     AKSNGLDVFVHDLASGPPPGIYDYLTCLDVLEHIAYSERAFRNLKSSFRKKIVISLPN
                     IGCLRCRIRLAIFGKFPLTVCIFHVNEHLRHWTLKDFRYWMKQEDMIIERMEGQYGLR
                     GFYRWFPSLFAHGLVYVMRRNESSRSGL"
     gene            16791..17180
                     /locus_tag="PKI64_02840"
     CDS             16791..17180
                     /locus_tag="PKI64_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017218415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOD99977.1"
                     /translation="MKRGITFGTFDVFHYGHLRILERARELCDYLIVGVSSDELNNSK
                     KGRMPYYSLQERMGILGALRVVDEVFVEESLELKGEYIRKYRADVLIMGDDWSGKFDE
                     FKALCEVVYLPRTPGISTTALIEQIRL"
     gene            17198..18322
                     /locus_tag="PKI64_02845"
     CDS             17198..18322
                     /locus_tag="PKI64_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOD99978.1"
                     /translation="MYALLALMPKRQTAVVYTWPAFEDTALALIPELEKTSLKSIYYL
                     AAAEVGGPMPQWGAKVRVLPKRSLRGLVAFLTSRWVFTTHQCYTSVFPKRVESVNLWH
                     GMPIKRIGWMAVKPGIVPRYKVDLATSAFWHPIIRACMRPWGNVLLNGLPRYDRFGAL
                     DSVEVRERLGLGAARSAKLVVWLPTYRRAVLGKPVRDGRNFDNVAQFSGFNSITFEQW
                     LRQHNFCCVIKPHPLSPIQERKASDRLLIADNGDLAARGLSLYPLLWAADVLLTDVSS
                     VYVDFLLLDRPVIHAFADRAEYESSRGFTFDWSDEFLAGPWVENMEQLQMELIRLKEG
                     HDAFSARRATLKKLFHEATHESASRSLLGQLGLLESAKSD"
     gene            complement(18506..19459)
                     /locus_tag="PKI64_02850"
     CDS             complement(18506..19459)
                     /locus_tag="PKI64_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD99979.1"
                     /translation="MAFLLYDSMQLAATTPAQPYPLPSRHHWFYFVSGLIFLALAKTK
                     NRLRGYVTPKPFNVTETERSIAYDLRIVDQWLARLQQATGSSNIEGKHILELGPGSDL
                     GIGLYLLAKGAASYSACDVHPLVYNTPDYFYDQLLQAIHIRVPSANMDSLRQAWLDWK
                     NGQPSKLRYVVRKDFDFCTAFGESSMDLVFSQAAFEHFDDIPAVANQLFRICKSGARI
                     VAEIDLKAHSRWIRDHDPNNIYRYPNWLYRWFYFPGIPNRLRPLHYRAAFEQAGWNSI
                     EIAPINYRPAHTQAAAGMHRAFIAPENQMHWLSVALTAQRP"
     gene            19633..19998
                     /locus_tag="PKI64_02855"
     CDS             19633..19998
                     /locus_tag="PKI64_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD99980.1"
                     /translation="MNVAIIGASNKPERYSHQAVLLLAAKGHAVYPIHPALTEIEGHP
                     VYKRLADIPVPVHTITMYVGPALSAGMDETLIAAKPQRVIFNPGTENPELESKLAAAG
                     IQVVQACTLVLLRTNQFDA"
     gene            20073..20876
                     /locus_tag="PKI64_02860"
     CDS             20073..20876
                     /locus_tag="PKI64_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOD99981.1"
                     /translation="MAAFTEWLKTLAPVNQALVGTLFTWLMTALGAAAIFLTRQPSRK
                     FQDGMLGFAAGVMIAASFWSLLAPAIEMSGGSWVPAVVGFLVGGVFLRLVDIALPHLH
                     VYAEDPEGRRSHWKRSILLVTAITLHNIPEGLAVGVAFGAAAIGESTYSVAGAIALTI
                     GIGIQNLPEGLAVAMPLQRSGLTRWKGFMYGQFSGMVEPLAGVLGAVAVIYAQPILPY
                     TLAFAAGAMIFVVVEDAIPEAQRSGNNDIATLGAMVGFALMMTLDVALG"
     gene            complement(20904..21980)
                     /locus_tag="PKI64_02865"
     CDS             complement(20904..21980)
                     /locus_tag="PKI64_02865"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOD99982.1"
                     /translation="MSNRHDITPGIENLPLEWLAGRRVGLVAHPASVVRDGRHSAAFL
                     RDQGINLTALFGPEHGFFGRGGAGETIADTAHADWNIPIFSLYGKTRAPTTPMLELVD
                     VIVFDIQTIACRAYTYVSTLHLLLETCARAGKTVIVADRPDPLMLTPPDGPLLDPACA
                     SFVGMIPTPFCYQMTPAETALFLRDELQLAALDLRIAPCRNLSRSLPLAAIYPNWQTT
                     SPAIIALANALCFPLTVFFEALPIMDHARGTPLAFQRLGSAHADLARLQLPPLPGLRV
                     TPAEYPDKNGHILSGLAFEVADPTAYRPAQAAFTLLHHLEEYLGPALWRFPGSRPDFY
                     AQLWGTRTPAPPWPDYSLRHLLYP"
     gene            22077..22874
                     /locus_tag="PKI64_02870"
     CDS             22077..22874
                     /locus_tag="PKI64_02870"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOD99983.1"
                     /translation="MTTALIRQHSALLQQAAEQIATARHLTAFTGAGISVESGIPPFR
                     GPGGIWNKYDPEVLDLHFFQRDPEQSWAAIREMFTCFLGTADQTPVLPNAAHQVLGEW
                     EAAGVLKVVITQNIDGLHAAAGSRNLVEYHGHCRSMTCLDCAKELPLDHTTTRETVPR
                     CDCGGLLKPDFIFFGEGIPSDMVERSEAAARQTDCMLLVGTSGAVYPAAFLPVLAKRL
                     GAFIIEINPEPTEFTSGVTDLHLPLPAGAALMELNTQVNRFLHNNKS"
     gene            22988..24010
                     /gene="gap"
                     /locus_tag="PKI64_02875"
     CDS             22988..24010
                     /gene="gap"
                     /locus_tag="PKI64_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD99984.1"
                     /translation="MAIKVGINGFGRIGRLVFRAAMENPQIEVVGINDLFDSKQLAYM
                     LKYDSIHGAFKGTVEAADNALVVNGKNIRLTAEKDPAALKWGDVGAEYICESTGFFLE
                     KAKAEGHIKAGAKRVVMSAPSKDDTPMFVMGVNHKSYAGEAFVSNASCTTNCLAPVAK
                     VLHDNWGIAEGLMTTVHATTATQKTVDGPGGKKDFRGGRAAAGNIIPSSTGAAKAVGK
                     VIPALKGKLTGMAFRVPTLNVSVVDLTVRLEKAAGADAESAYAAIKAAMKKASEGEMK
                     GILGYTTEDVVSTDFNHDARTSIFDAGAGIMLNPTFAKVVSWYDNEWAYSNKLLDLIV
                     HMNSVK"
     gene            24084..25328
                     /locus_tag="PKI64_02880"
     CDS             24084..25328
                     /locus_tag="PKI64_02880"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOD99985.1"
                     /translation="MNKKTLQDVELSGKRVVMRVDFNVPLKNGVISDDTRIQAALPSI
                     RHILGQGARLVLMSHLGRPKGKGYEAEFSLKPVAEHLAKVLGQPVAFAADCLAADAEV
                     AALQPGQVLLLENTRFYKEEEAKVKKTDDISEEDYKAQKAALKEKQKAMAKKLASYGD
                     VFVNDAFGAAHRAHASTAVICKYIPVSVSGFLLEKEIRYLGNAVNHPERPFVAILGGA
                     KVSDKLAVVNNLLDKVDTLIIGGGMAYTFLKAQGHDVGQSLCELDQLDYARDMLAKAK
                     ANGKKLLLPVDNVAADKFAADAATQVVGNDIPAGWMALDIGPKTIAAYAAAIQGAKTV
                     VWNGPMGCFEMAPFAAGTFGVCEAVAASGATSIIGGGDSVSAVNKSGLAAKMTHISTG
                     GGASLEYLEGKELPGVAALSDK"
     gene            25365..26120
                     /gene="tpiA"
                     /locus_tag="PKI64_02885"
     CDS             25365..26120
                     /gene="tpiA"
                     /locus_tag="PKI64_02885"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOD99986.1"
                     /translation="MRKKIVAGNWKMNMTTAEAAALVNTLKADLANFREVEVVVCPPF
                     TALSTVSELVHNTHIDLGAQNMHWEKNGAFTGEICASMLRELYCHYVILGHSERRQYF
                     GETDAIVNRKTKAALAASLHPIVCVGETLQEREAGKVEEVITTQVKGSLAGLSAKEVL
                     ESVIAYEPVWAIGTGKTATSDQAQEVHALIRNVLTGMFDATVAQSVRIQYGGSVKPAN
                     AKELFSMPDIDGGLIGGAALDAKSFIDIVRAAV"
     gene            26156..26755
                     /gene="secG"
                     /locus_tag="PKI64_02890"
     CDS             26156..26755
                     /gene="secG"
                     /locus_tag="PKI64_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOD99987.1"
                     /translation="MSILITLLLLVEVITAFLLIVVILAQKSKDQGLGMAFGSGMGES
                     LFGSRAGNVLTRMTVTLIIVFTITTVVLGILFSGGREASRSGSVMDTVDMSRQVPAAP
                     VAVPAPSAPLLPEEQPLVATEEVPSAAQTLQVDAEGNLQPVEGASGEAVVPETVVAVE
                     ETAAEAGSPAEVEVEAVVVETPAEAPTAPAEVEEAAPQD"
     gene            26770..>27202
                     /locus_tag="PKI64_02895"
     CDS             26770..>27202
                     /locus_tag="PKI64_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD99988.1"
                     /translation="MTTSPRTACAGILFALLLLAAGGCGSAHQTEQATDTLHLTTMRI
                     RGFDPAHSVDEASVRAAGLVYEGLLQYSYWTRPYRVEPLLAATMPELSADGKTWRFLL
                     RSGIYFADDPCFVASGGKGRELMAADVAYSIRRIADTKVGSS"
BASE COUNT         5562 a         7054 c         8411 g         6175 t
ORIGIN      
        1 acatcaccat gatgcatgaa cgaaacccca tagcatctac aatccaaccc ggatgttttt
       61 gtagggcggg aatggttgag gaacgaaacc ttcccgccgc aaaaacggct ggaaggtctg
      121 cgaccattcc agccctacaa tccaaccaca acgagttacc acacgaattt cggggcgcgc
      181 gcaactcagg gcgcgcggtc aggcaatggc cgcatagccc aacgcaaaag cgcgcaagtt
      241 gacgtccagc aattgggccg ggatacgttc gcgcatggcg gcctgccagt ggtcttcgcc
      301 aaatgggacg agcttggcca gcgcacccat caccacgacg ttcgctgtgc gcagattccc
      361 tgcttgcacg gccagctcgt tggcggccag caggatcaag ccggggtagg cggcaagccg
      421 ctcatccaca ttttgcgcag cgggttgcaa gccgctggat accgtgaccg gaatgcggtc
      481 catgttgttg accagaatct tggcgcccgg acgggccaga tgagcccagc gcagggcttc
      541 cagccgctca aatgaaacga gaatatccgt ctccccggtg ggaatgatgg gcgaatggac
      601 gcgtgtgcca aaccggactt ggctgatcac ggaaccgccg cgctgcgaca tgccatggac
      661 ttccgatttt tttacatcat agcccgcgtg catcgcggcc atggccagaa tgtcggacgt
      721 cagcaaaatg ccctgcccgc cgacgcccac cagcgtgacg ttgaacaact tgtcgggaaa
      781 tgtggaagag gtcatggcga gtcctttatt tgaccgggga gatggcgcca aacttgcaaa
      841 cctgcgcgca gaggttgcag ccggtgcaga actgggggtc aatcttggct ttgaacaagc
      901 gcccgttggc atccttgggc gcgccgcgga cgatggttgg gcagccgatg cggaaacaag
      961 agccgcatgc agtgcatttg acctcgtcca ccgtgttgat gtatttgccg gcccatttga
     1021 cgtgcaacac gcaggggccg cggaccacca ccacggccgg ttcaccgcag tcgagacatt
     1081 ctttgaagac tttttccagg ttggccagat cgtagggttc cacttcgtgg acgcgctgga
     1141 ttccgaaggt gcgagcgatg tcggcaatat tgactgccgg tgattccacg cccaacaacg
     1201 tgtgcccgct gcccgggttt tcctggtggc ccgtcatggc cgtgatgcgg ttatcgaaca
     1261 ccatgaccgt gatcgggatg ttgttatagg ccacatccag cagtccagtc atgccggagt
     1321 ggaagaaggt ggagtcgccc atcaccgccg cgatgcgctg gttgggcagg ccggccttgc
     1381 gcatgccgat ggctgtgcca atcgccgcgc ccatgcagac gcaggtgtcc atggcggaaa
     1441 gcggtggggc cgagcccaac gtatagcagc cgatatcgcc acagaccgtg gccttgagtt
     1501 tggcgaggat atgaaatgcc ccgcggtgcg agcagccgga gcaaagcacc ggcgggcgca
     1561 caggcaagcc ggtttccggt tgcgcggtgg ggggcggggg cgtgccgagc agctcggcgc
     1621 gcagggcgcc caagcgcacg gggtccagct ccatcatgtg caattcggtt ttaggttcca
     1681 ccaccggaat gcccgccgcg cggatttgct cggcaagaac ggggtcggat tcttcaatca
     1741 ccaccagccg ttgcacgccg gcggcaaatg cccggatttt ttccagcggc ggcggatagg
     1801 agaggcccac cttgaaaact ggcgcggtgg ggaagacttc cttcacatac tgataggcca
     1861 cgccggaggc gacaaaaccg atatcgcccg tgccggaatc cgcacgattg aagggcgatt
     1921 cttccgtcag cgcccgcagc gcttgggaac gtttttcgac cgcaaagcgc atcaaccgcc
     1981 cgtgcgccgg caccgcgaca ttgcgctggg cattttttac gtaagggatg accggcgttt
     2041 cgctgcgctc cccggagccg atatccacca ctgtggaggt gtgggcggtg cgggtggtgg
     2101 tgcggacaat gaccggcacc tgatatttct cgctcagctc aaaaccggcg caaaccatgt
     2161 cgtaggcctc ttgcgaatca gccggctcaa agagcaggca tttggcaaat ttggccaacg
     2221 aacggttatc ctgctcgttt tgggaagaat gcaggctagg atcatcggca ttgatgatca
     2281 ccatgccacc gatggcgccg atataggtga acgtcatcaa cgggtccatg gcaacattga
     2341 ggcccacgtg cttcatggcc accagcgcgc gggtgccggc cagcgaagcg ccaatgccga
     2401 tttccatggc caccttctca ttgaccgacc actcgcaatt gatggcgccg gcaaattcgg
     2461 cgatatgctc catgatttca gtggaggggg tgccaggata tgcggcggcc acgcggcagc
     2521 cggcgcgata ggcagcccag gcgatggctt ggttggcgga aagcagtttt ttcatacagg
     2581 gtccggggtc ggggttcgca aaacaagggg ccgactgtag cacccgtgcg ccgccccgca
     2641 aagcaggaaa agccgtgcgc atttccggcg ctttacaaac cccggccaaa tggactagat
     2701 tgcccccgtt ttcaatcccc ataaccaaga ggaaaaaaga atgaaaaaac tgattttgtt
     2761 tggctgtgcg gccttgctgg cggcgggcgt gtcgtttggc ggcgtggtga gcgaggatcc
     2821 cggcgaactg tccgtggtcg gccaagtgtt tttcccggat tccgcggact gcgatttgtt
     2881 caagatcggt tacggcgcgc aagtggccta ccgcgagtgg ttcagtttcc cgtggggcgc
     2941 gggcctcacg ctgggcgttt cgcagtggca ggtggacagc aagtccgatg ctttcaagta
     3001 tgatatgctg agcaattacg atggcgatgt gctggtgatc cccatcgggc tggcgttgta
     3061 ttttaacgtg attgattggg acaactggaa cctgcttctg gaaaccggcc tgcagtacgc
     3121 cattgtcaat tccagcgcca cggtgttcaa caacgagccc gaagcccaga agaaagaaga
     3181 tgtggatatt gaaggcgcca tcctctggaa tctcggcgcg gagtttgaat acatggtggc
     3241 cgagaacatg tatctgcaag tggccgccgg ctatcaactc gacctgatga agggcgacac
     3301 cgaatatgcc ggtcggtccg tgcgtgatgc ttcgtttgct gccggttacg cccggatcgg
     3361 cgcgaagttc ttgttctaag ccgggaccag gttgaagaaa cctggccaac gcaaggcacg
     3421 tccgctccgg tggacgtgcc tttgtcgtga atgagcttac atgaatcgtt gctttctacc
     3481 tgcagaccgc tggggccagc ctgaggctga attatcggcc gaggagtccc accatctggc
     3541 cggagttctg cgtgcccaag ccggtcagcg catcgggttg ctggatggcg cgggccgcac
     3601 gggcgaagcc gaggtggtgg caccacacaa aaaacacacc cgcatccgta ttctttcgcg
     3661 gcagcaggcg ccggcaaacg tcccacgccg gatattggcc caagccttgg tgcgcgaaca
     3721 acagatggat tggttgatcc agaaagctgt cgagttgggc gtacatgaaa tctggccgtt
     3781 gcaaaccgat catgctgtgg tcaaggtgcg ggccgaagac ggcggaaaaa aggtggcacg
     3841 ttggcaatct gtcgctttgg cggcttgcaa gcaaagcggc aacccatggc tgcccacgat
     3901 ccggccggtg cagaaattgg caggcgcatt ggccggtttg ccgccagcgc cggcagccgc
     3961 ctgctttggc gcgttgcagt ccggggccat gcccctgcca gcttatttga ccggattgcg
     4021 ccaagccgtg tgctcgacca ttaccatgtt catcggcccc gaaggcgatt tcagccaggc
     4081 ggaggtggct gccatgttgg ccgccggagt gcaaccggtc acctttggcc cgttgatctt
     4141 tcgggtggaa accgcggcac ttttcatttt gtccgccgtg cattatgctt ggatgaagta
     4201 gcccaaggtc gttatgcagg aattggcacc acaacctttc accattcaag gcgagccggc
     4261 gttcacgttc accgccttgc ccgcgatagg gagtgttgcg cccgattttc gcttggccga
     4321 tcagcatttt cgcgactggg cattggccga ctttgccggc aagaaaaaga ttctcaacat
     4381 cgtgccctcg ctcgacactc cgatttgtca gctctccgcg cagaagttca acgaagcggt
     4441 ggccaagcgc gatgacgctg tggtgttgac gatttcggct gacttgccat tcgcccaagc
     4501 ccgtttttgc gccaattgga aaactcccaa tggccagttc ctgtccactt tccgttcccc
     4561 ggaatttggg atgaattatg gcacgttgat cgtggagcac gccctgatgg ggctgcaagc
     4621 gcgcgccgtg attgtgttgg atgaacataa tgtggtgcgc catgtccagt tggtgccgga
     4681 attaaagcag gagcccgatt atacggctgc gttggccgcc ttggagtcgg tggacgcatg
     4741 aataccatcg tccaatcgct ggcgtgggga ttgagccggt tggctgggcc cgtggcctat
     4801 cctcctgaaa ccgatgcccc gtatgctccg ggggaagcct atcccgagtt ggccggccag
     4861 actcgccccg gcggccagcc caatcccatc tttgccgccg tgcgggaggc aatgcacaaa
     4921 atgggcttgg atgccgacca ctttgggacg cccgcatgga atccgcttgg catgctggtc
     4981 aaacccggcg gcaacatcgt ggtcaaaccc aactgggtat tgcacaaaaa tctggggcag
     5041 ggcggcatgg agtgcatgat tacgcatccg tccgttttgc gtgcggtgct ggaatatgtg
     5101 tttttgacga agcccgcgct ggtggtcctg ggagatgcgc ccttgcaggg ctgtgatttt
     5161 gagcggctgc tggactatgc cggcctgcgt gccgtgattg cggaattcca gaaccggggc
     5221 ttgccgctgg tgatcaaaga ttttcgacgc acgattctgc gcgaggaagg cccgctcaaa
     5281 aaggtggctg aagagttgcg cgcggaagac cagtacgaat tggtggattt gggcgcggat
     5341 agcctcttgg aagcgatttc cgccgattgg aaaaagtttc gggtcaccat gtatgacccg
     5401 cgcctgatgt ggcggcatca tcgcccgggc cggcaccggt acttggtggc caaagaaatc
     5461 ttgaatgccg atttggtcgt caacgtgcca aagctcaaag cccacaaaaa ggctggggtc
     5521 acttgttgcc ttaaaaacct gatcggcatc aacggcaaca aggaattcct gccgcaccat
     5581 cgcaagggtg cagtgtccgc cggcaagggc gataattatc caaaccgcca ttggctggtc
     5641 acgttggcgg agcatctgtt ggatgtgatc aactgtctgc ggggatggcc gcgattgtat
     5701 cggcagggtg aacggcttgt ctggtggttc ttgcactttg ccgagagaaa agatgccact
     5761 acgcaaattg aaggcagttg gcatggcaac gataccatct ggcgcacctg ccttgatttg
     5821 aaccgggcgc tcctttttgc tgattgcgcc gggcagatga ccgacaccct gcaacgccaa
     5881 gaaatctcca tcgtggatgc tgtcatcacg gggcaggggg aggggccgct ggcgccggat
     5941 ccgttggaaa ctggcgccat cttggcggcg cataatccgg ccatcggcga cttggtggcc
     6001 acccgcctgc tcggttttgt gccggaaaag attgcgctgc tgcgccacgc ctgcgacgac
     6061 atgaaatggc gtatttgcgc agccccccct gtggttcccg agttcacgcc acccttccca
     6121 ccagcccgtg cccccaaagg ctgggctggc catattgaag caacgggggc cttggttccg
     6181 ccgaattcag actcatgaat gtcggccggt cattacgctg gcacaacgcg cgggtgaatg
     6241 gggccagaaa aatcgccaat ctgttatggc ggctgcgcta taacggcaat gaattcagta
     6301 tcattagcaa taactgctgg ggcggggcca tttaccaaag tctgcatcgg ccctatttgt
     6361 cgccgtttgc gggcatcttt cttatggccc cgtgttattt gcggttattg gctgatttgc
     6421 gtcattactt gagccgaccg ccacgccaaa ccaccaaatc gcaatatgcg tccgtgcatc
     6481 gcgtgtatgg cccaacactg ccttatccgc tgggaatcct tgacgataat attgagattc
     6541 acttcctcca ttacgctgcg tgggaagaag cgctggacaa atggcaacgc cgcaccgaac
     6601 gaatcaactg ggaccggctg tttatcaaat ttggcgacca aaacgatagt tcacttggtg
     6661 ccgtacagca gtttctccaa ttgtcgtacg aacgaaaact gtgcctgacc aatcgccaag
     6721 aggtccatgg ccccgctgtg gtggccatga agggtatagg accgaccact cgctttcagg
     6781 aagacgtgga atttcgccgg tacatggatg tcccggactg gttgggtgaa gggcgggcac
     6841 ggggtggggc gggactacgg cggctgagcc attgggcgga tggccattat gttcaggcct
     6901 atcaagagcg aatggccgca cttctggcca cgggtgaatc cctcccgatg aatctggatg
     6961 cgcaattggc ggctatggat caatctccac cagttcgccc acctcaattg taaacatgag
     7021 cctgcgcatc aagtccttca agcaagcggc cttttggtct acggcaatca acgcgttcag
     7081 tcaggggttg gcgcttctct tcagcatttt gatggcgaag tattttggtg cgcaagaaag
     7141 caccgatatt ctgtatttct gcttgggcat ctttgttttg ctcggcggtc tgttgcaggt
     7201 ggtaaatgtc agcgtactga ttcccgaaac catgcgccgc cggcaccaag tgagcgaaca
     7261 ggacgccatg gcatttatta accgtttttt tgcggtattt gtgctgatga ttggggccgt
     7321 gaccgtgctg tcgctatcga atccggtgct cgctctatcg gccatatctc gtttcgatat
     7381 tgcggccgta gagaggaata tgcgtttggc ccagtggctg ttgttgagct tcccattgca
     7441 aatgacggca caattgctgc tggatattct ggtttcttac aaatttttga tgctgccggc
     7501 acttttgtcg tgtgtaaacc ggctaatcaa catccttttt gtcgtggtgt tttaccggcg
     7561 ctttggagtg gtatcggttg ccatgggaat ggttgtcggc ttcagcttgc aagtggcgct
     7621 gaacgcgttt ttactgcggc gtaagataca ctggaagttg agcgcttggc ggacccgtat
     7681 tacgcgccaa acctattgga atattctttg ggtggaattg ggtactctgt ctggagtatt
     7741 agcgagctat ctgccgattt atctattttc cggctttgct gcgggagtag tgacgattct
     7801 caattacgcg cgtcgaatga gcctgatgcc cgtggaactc ctgacggtcc aggtatcatc
     7861 cgtgattgcc gtcaaattca acgagcaagc tgcgatgcgc aactttgcag gcatggggaa
     7921 aagttttggc tggttgcagc gccagcaaat attttttctc acccctttgg cctttttact
     7981 gggcattgcc ggttatccga ttatttcctt gttatacggc catggtgcat ttgatgccaa
     8041 tgcggtggcg cagaccgccc gcttgttttc ggtgttgatg cttatgctgc cgtttttatc
     8101 ggtggacaat gcggttgccc gtctgtttgt agcgcatcag gaagttgcgt tgggcacccg
     8161 ttaccaggtg atgggcagca ttctcaatgc ggttttgatt tttattttcg tcaagtggaa
     8221 aggaaccatg gggtttgcgg tggccatgct gttgacacgg ctactgtatt tgttgatctt
     8281 gtctgccata ttttccaaac gcttcagacc gatcacactg tggccgacta ctcgttcgat
     8341 tctactaacc tttgcctatt gcggcgtatt ggcatgcggt gcgttgtggc tggcctcttc
     8401 gccatttttc gtgacgttgg cggcaagttg gcgcggagta ctagctgccg gtattttcat
     8461 tgctggatat ttggcatcac ttgtggtgtg tccaccagac cgtctggcaa ggaatgaagc
     8521 gattacgctt ggggtggagt tgtgggcgcg aattcggcat attcactcga aatggacgga
     8581 aacggtgccc tcatgaatca agatttacaa attggaatac aaaaacccgc agggctgaat
     8641 tttgccgaga ggtgggcggt ggcgcttgaa aaaagcgggt gtcgtgttcg ttggctggat
     8701 ttgttgggaa ccgatccgtt atctcagata caaggctgcg acggcatcat gtggcattgg
     8761 ttccattatc cgcatgaaat ccgtttggct gcactgccga ttttgcgagt gatcgaagag
     8821 cagttacgga ttcccgtatt tcccgacatg gccaccgcgt ggcattatga tgataaaatc
     8881 gcccagagct atttgttgaa ggctttgaat atccctcatc cccaaacgtg ggtgttttgg
     8941 cgtaaggccg atgcgctgag atgggcggaa actgctcaat accccgtggt ggccaagttg
     9001 agcgggggag caggcagtat gaatgtccgg ctattgcgtt ctgcgaaaga agcgcggcaa
     9061 tatgtgaacg cttgtttctc cggcgccgga attgtggggc gacccgcgct tccgcgtgga
     9121 gccttgtcgg cgacattggc ccgattgaag cgggcattga aacgcgtggc acaagccgga
     9181 ccatacgttt tggcgaatcg gtttccggcg ctgcccgacc aaacctattg gatgccacaa
     9241 aagaattatg ccctgtttca ggaattcctt ccggacaacg cgcacgacac gagggttaca
     9301 gtcattggta accgggcgtt cgcatttcgc cgttttaacc gccctaatga tttccgggca
     9361 tcgggtagtg gcaacttgtg tgttgatcca aacggcatag acatgcggtg catagcggcg
     9421 gcgtttgatg ccgccgctaa acttaaatcc caatccatgg cctttgatta tcttttccgg
     9481 ggggctgaac gcgaaccggt agtgggcgaa ataagctatt gttatgcgga ctgggctgta
     9541 gaatcctgtc ccggtcactg ggattccaat ctgacttggc atgcaggccg gatgtggccg
     9601 caagaggcgc atgtcgaaga ttttgtcgcc cgaattgtcg gagcacgcac actcccatga
     9661 agattttgat ggtcgtggct cattttatgc cggtgcaccg cggcggtgcc gagatgcaat
     9721 gttggaaaca ggcaaaagcc ttggcgcaac gcgggcataa agtgacggtg ttgacgccgt
     9781 ggtggagcct ctcaagtaag cgaagcgagt gggtggatgg ggtgcggatt cgccgtatgg
     9841 gttttttgct accactgact tggctggcgc ggcgatggca cgcccgctta aagccacctc
     9901 ccgcggaggg ggggggggag gttgtatctg ccacacccaa agcgcgcaag aagcgctttc
     9961 gctggatgtc gctgacggaa cgtccggggc tgttttcatt tattttggaa gtggcatgtg
    10021 cgattcgatt cagacgcctt cgcccagacg ttattcacgt acatgaaagt cattggatcg
    10081 ccggattcgc tcattggatg gcggagaggt tggatgttcc ggtcttttgc aaagagggac
    10141 tattgccggt gttggatttt ggcggtgctc aacatgttcc gtggcgggag cggtggcggg
    10201 agcggcgcac gaaatgtcga tttattgcaa tgaccgatgc tattcaagac gcgttaataa
    10261 aggctgggat tgctcctgat cagatcgcct gcgtgccgaa tggggtggaa atacccgaga
    10321 ggaaagcaca accgcagcta tatggtgatg ctctctatgt ggggaatttg tcgcaagggg
    10381 cagcacacaa gggctttgat gtgttgctca aggcaatggg agaggttgtg aaagaagcgc
    10441 ccgaaataaa actgcgaatt tgcggtgcgg gtgtggccac aggctggcaa gcaatggcgg
    10501 atgagttagg atgtggtacc gcggtggagt ttgtggggcg cgtggaagat ttgacagaat
    10561 accaccacca ttcgggcttt ctggtattgc cctctcggaa ggaaggtatc tccaatgcgt
    10621 tgttggaagc tcaagcagcc ggtttgccag cgattgtcac ggatattccc ggcaatacgg
    10681 ccgtgataca gcacggtttc aatggtttag tggtaccggt cggtgatgcc gtggctttgg
    10741 cacgggccat cgtggaaatg catcgttcga cggaattgcg cgaacgcctt ggtcacgcag
    10801 cccggcagca ggcagaagca cgttttgcca taagccgtgt ggctgagcag ttggaagagc
    10861 tgtatcacac tgctcttgtg gcaaccacgc ccgacggctt ccaggattga ttttccagca
    10921 ccggctccgc atattgctcc caagattgtc atgagtaccg ccccgtccaa ctttccaacc
    10981 agccagcgca cgacctatca gtttccggac tatcgctgga tcggctggtt gacggttgcg
    11041 ggatttgcgc ttggcctgac cgtcatgttt tttattgccg gaatgtttgg aataccggcg
    11101 ccattgggtt gggctttttt ggtcatcctg tttgcgttgg gagccctgct tctggatcgg
    11161 cccaagctgt tgctttcctt tatgattttt tattttttat ttatgccgag taaccggatg
    11221 cttggtttag tcagcttacc cttgcccgat tttcttgatg aacttttctt tttgccattc
    11281 attgccgtga ttgcgatgaa ctggatccaa cggcggcaac taactgaagc tacctttttc
    11341 cctgtcgcct tcattggggt ggcgatcatg agttcgattg tcaatgatcg tccttcggtc
    11401 tttacagccc tccaggtcac gttgattatg ctgaaatcct atattttatg gtatttctgc
    11461 cggttgacgt gcacctttag caacacgcgc caactcatgc gctgggtgtg gttttatgtg
    11521 atttatgtcg cgtttcagtt tttgtacaac atactatggc agcatggccc ttggccgcat
    11581 taccatccgg atcgcagcgg tggggtcttc gggccagatg gcaatggcgc cgcccacttg
    11641 gtgggttact tgagtgtatt cgccttgatg ttgttgacgg gctggtgggt cagcagcgcc
    11701 caccgtagca ccattcgcca acgaattgcc gtggtcttgc tgacgctggt gatcgcctac
    11761 aacttgatct tcatgaccga tacgaagcat gcgctcctga tgatgccgct tgcatttatt
    11821 ccattcctgc tgcaccctaa atttccagtc cgattgcgtg tgtggttgct tctggccggt
    11881 gcggtgtttg gtcttgccac catggtttat ttcaatgttg ccgttggcca ggtcaatatt
    11941 cgccgatacc tcaatgggat gcgcgattcg ccgaaaggcg aaatgttcta tgcggtcacc
    12001 aaggattttc gttttttagt gcgctatcct ttattggggg ccggaccggg ttgctttggc
    12061 tccccccaat cggttgatgc ccgaaccccc ttggcccgcc gttatattat tccctaccgc
    12121 gatgaaatac ggcgtttgga ttactttggc aaaggacttg gcggcacccg tgcggcggcc
    12181 tcggttctgg gaatgcccca gtcggattta tttacactga tgggcgagtt cggttggctg
    12241 ggggctcttg tatattacag ttttttgggg tgggtggtgt tacggttctg gcaaaaatct
    12301 gcggccttgc cgctgcgaca ccccttatcc gggctatata tggcttgttg ttgttgtgtt
    12361 atctttttgt cattcgtgac ggtccttgtt cccgcagcca ctattccact gctggtgttc
    12421 ccgttgtgga tgctaattgc gcgaatgtgg gatatgccgg ggaatgaaat ggagatggcg
    12481 gatggcacct gatggtcccc cggccgaaac cctctattct gctgcgccgt gggccaatcg
    12541 gacgggcgcc cccatttgtg cactagaaca tttgatcgca ctgcgtgatt attttggtcc
    12601 gatttgtctt atcttgcctg agcatggccc gcttgaaaaa ttagccgcgg cagcaaatgt
    12661 ctccgtgtgg gtttccccat tggtctataa aggattgcgt cagggaggat tttggcggtt
    12721 ctggtgcggc atcggccctg tgttacggtc ccgatggcaa tatctccgca gactgattcg
    12781 tcaattacgc agccaaccgg ggattctcca tattcatagc cggagcactc atttaccata
    12841 cgcgttattg gctggacggc tggcccgcat tccgatggta gtgactatcc acgagccatg
    12901 ggcaggaggc gtcgaggcgt ggttggatgt tctattgatt cattggcttg ccgaccgggt
    12961 gattcttgtc tcccagaaca tggccgatac ttacccacgt tttttgtgtc gtcgtgcagt
    13021 aatttcatac tatttcagct cggtaccggc actttcggct cgtcctgagt cacgctcgcc
    13081 attgcgaata ggaatgttgg cccggatggg gcaccgtaag ggcgctgata tttttctcgc
    13141 cacctgccgg ctcctgcggg aacgcggcat cccttgtgaa gcctgggtgg cgggtggctg
    13201 gaattcagag gccgagcaaa aggtggccgc cgattatatg gcggcccacc aattgcaatc
    13261 ggtcgttcgc gatcttggtt tggtggagga tatgccggcg ctctatcggc agttggatgt
    13321 tttgtttttg ccggcgcgcc gcgacccttt gccacgggtg gtgatggagg ccatgtgcca
    13381 cggcttgccg gtggttgcca gccgggtgga cggaatccca gagatggtgg tggatggcga
    13441 aaccggcatc ctggttgagc cggagaatgt tgagggtttt gctgatgccc tgatgcggct
    13501 gctggcggat ccggaattac gccaacgaat gggagccgcg gcccatgcgc gcgcgcagaa
    13561 aatcttcact gtcgaggctt atcgcggcgc aatgttaaaa gcatataagg gtctactgcc
    13621 gaccaaacca taattgggct tgtggggcag tcgccggatg ggatagtttc gcgcgaaaga
    13681 atgggcagca ggcaatgagg gtactcgtaa caggtgacgc aggctttatt ggttcccatg
    13741 tggaaagagt attgctgaag cagggcacaa gcgatcgtgt tgggtgacct gcagaactaa
    13801 ccgatgaacg cccaatcacc caaagtaggc attgccctct taaactggaa ccaatacgac
    13861 gatacagccc tttgccttga atcgttgcgc gaatcaaaat ttcgtcctgc catcatactc
    13921 gtgtatgaca acggttcgac cgatgggtca gcggagcgac taaaggcgaa cttccccgag
    13981 atacaactgg ttgttggtgg tcaaaatttc gggttttcgg aaggcaacaa tcgcgctgtc
    14041 cagaatctgc tagaagcggg tatggacttg gtctgggtgc tcaacaatga cacaaaagtg
    14101 tcacctgatt gcctgggatg gcttgttcac tcgctcgatg aacagccaga agtgggtgcg
    14161 gttagcggca aaatttggtt catggaagag actcggcctc tttgctatgc cggtggcatt
    14221 ttcaaccgct ggaatttcaa cacaaccttt ctcggctttc gcgaacctga cagcggacag
    14281 tatgatatcg gaggagacac cactattttt tcggggtgct gcatgttgat ccgtgccgag
    14341 gtgatccgga aaattggact cttcaaccgc ggattttttg cctatgccga ggacatcgac
    14401 tggagcttgc gcgcgcaggc ctccggcatc cggctgtact acgaacctcg cgccactctc
    14461 tggcataaaa tgtttggctc aacggtgaag acgaagggtg gcgccaaaaa atcatccgaa
    14521 ttcgtcgagt tcctttcagc gcggaatcgc tttttattgg tgcgcttgca cacgcgtccg
    14581 tggaacgtac gacgttgctt cgctttgtcg tggcatctct gggttcgcgg cattcctcgc
    14641 gcaattggtc taatgttgtt gccgagccgt cgtgctgtcg ggtgggcacg tctcaaaggg
    14701 ttatgggcgg ggttgtggat gcaccccgat ccggcaaact gtatgttaaa ttcgcctgca
    14761 tcatgagagt ttactgccag agcccttatg catttgttag tgaaaagtgc gttgaagagc
    14821 gtgttacagg ccgcagaaat tgaacacaat tatttgtgtg taaatcgggt agcgggatat
    14881 attatgggtc ttgtcgtttt cattaaggac caattgggtt ccgtttttca ggatacggca
    14941 tgagctcgat acccccgact atacactatt gctggtttgg aggtggccca aagccagcgc
    15001 tggcggagcg gtgtctggct tcatggagac gttatctccc ggagcatgaa cttcgcgaat
    15061 ggaacgaggg ttcttttgat atctcgaacc ctccttatgt gcgcgaagcg tatcgcttga
    15121 aaaaatatgc atttgtcgcg gattatgttc gcctgcacgc tttgcagacc cacggtggta
    15181 tctatctgga cacggatgtt gaactcttgc ggaatccaga tacggtgttg acggcgcccg
    15241 ccgttttggg ttttgagacg cctgaacgcg tatgtagtgc gtttctggcg tttgcacccg
    15301 gacacgagtt aattcacgaa tggctggaaa cctaccggaa tcggtcattt ctccgggacg
    15361 gaaagccgga tgaaaccaca aacgtgcagg tgttgacgaa gttgttgcga tcccgggggc
    15421 tggttttgga tgggacggag cagcggttga ggggcgggat cgtcgtgtat ccgcaagacg
    15481 tgttcagccc ctatacctat ggtgaacgca agccgcgcac gacggcgcgc acagtggcaa
    15541 tccattggtt ggaggctagt tgggcgaccg ggagcacgcg cgcgaaactg gtgggtattc
    15601 aggcgatcaa gcgcctgttt ggtgcgggat tctaccattg gctgcgccgc atctggtcgg
    15661 cgtcaagacg ccgcagagct aacagaggcg atcggctcgc ctagtcggag ttccccgccg
    15721 aggaggagcc tagtctcccg gaactggggc cctgaacggg cggatgtcgc cgagtgcatg
    15781 aaatgcagcg tgtgcgagac gccttgtact cagtatcagc tctttgtttt ttgcctcaaa
    15841 accggcggca tttaaaaata gcgcaaagcg ggggcgttcg ggcgccgggt gcacgatact
    15901 tacaagccag agcgtgaaga ctcgttacgt ctcatgacat agaccaagcc gtgggcaaag
    15961 agacttggga accagcgata aaacccgcgc agcccgtact gtccttccat gcgttcgatg
    16021 atcatatctt cttgtttcat ccaatagcga aaatccttca gggtccagtg gcgcagatgt
    16081 tcgttgacat gaaaaatgca gacagtgagc gggaattttc caaatattgc caggcgtatg
    16141 cgacagcgca aacaaccgat attgggcagg gatatcacaa tcttttttcg gaagctggac
    16201 tttaaattgc gaaaagcccg ctcggagtag gcgatatgtt ccaagacatc caaacaagtg
    16261 agatagtcgt aaatccccgg aggagggccg ctggccaaat catgaacaaa cacatccagt
    16321 ccattgcttt tggcccgttc aaccccaatc gcagaagtgt cgcacccctt aagaatcaga
    16381 tcaggtcgtt tttcgcgaag atagcttaaa aattcaccat tgccgcatcc aacatccaag
    16441 acggttgaat tcagctcaaa cgtttcggca gcgacttccc accggcggta aatgacggga
    16501 actttccccc ttttttccca gtagttgtcg taatcggtga attcgtttag aggaggcggc
    16561 tgcaactgat aagcaagaat cgaatgcaga gtgcggaaag gcttggttaa agctccccag
    16621 atggattgaa atatattcat tgctgaacaa aaccatataa caagtgtgga ggtcgagcgg
    16681 atatttcacc gtttacatcg ctttcccatg cgccgagacg atattgctct ttctcccgtt
    16741 gaaggtttgt gacaggatga ctatttatca ggacggcaaa ggaaaacacg atgaaacgag
    16801 gcattacatt tggaacgttt gatgtgtttc attatgggca tttgcggata ttggagcggg
    16861 cgcgagagct gtgcgactac ttgatcgttg gggtatcgtc ggatgagttg aacaactcga
    16921 aaaaggggcg tatgccctat tattcgctgc aggagcggat gggcatcctg ggcgcattgc
    16981 gggtggtaga cgaggtgttt gtggaggaat cgctagagtt gaagggcgag tatatccgaa
    17041 agtatcgggc ggatgtgctg attatgggcg acgactggtc tggcaaattt gatgagttca
    17101 aagccctgtg cgaggtggtc tatctgccgc gtacgccggg tatttcaacc acggccttga
    17161 ttgagcaaat tcgcctatga atttacgctc ccacctgttg tatgcgctgc tcgcgttgat
    17221 gcccaaacgc caaacggcgg tggtgtacac ttggccggcg ttcgaggaca cggcgctggc
    17281 attgattccc gagttggaga aaacttcgct gaaatcaatc tattatctgg ccgctgcaga
    17341 ggtggggggg cctatgccac aatggggcgc caaggtacgg gttctgccga agcggtcgtt
    17401 acgtggactg gtcgcatttt tgacgagccg ctgggtgttt accacgcacc agtgttacac
    17461 aagcgtgttt ccgaagcgtg tggaatcggt gaatttgtgg cacgggatgc ccatcaaaag
    17521 aattggctgg atggcggtga aaccgggcat tgtgccgcga tataaggttg atttggcgac
    17581 atcggctttt tggcatccga ttattcgcgc gtgcatgagg ccgtggggca atgtgttact
    17641 gaacgggctg ccgcgttatg accgttttgg cgcgctggac tccgtggagg ttcgtgaacg
    17701 cttggggttg ggagcggcga gatcggcaaa acttgttgtg tggttgccga cctaccgccg
    17761 tgcggttctt ggtaaaccgg ttcgtgacgg gcggaacttt gacaatgttg cgcagttttc
    17821 agggtttaat tctatcacgt ttgaacaatg gctgcggcaa cataattttt gctgcgtaat
    17881 caagcctcat cctttatcgc caattcagga gcggaaagcc agcgaccgcc tactgattgc
    17941 ggataacggg gatttggcgg cacgcggttt gtcgctctac ccactgttgt gggcggcgga
    18001 cgtcctgttg accgatgtct ccagcgttta tgttgatttt ctcctgctgg atcgaccggt
    18061 gattcacgcg tttgcagacc gggcggagta tgagtcgagt cggggattca cttttgattg
    18121 gagcgacgaa tttttggcgg gcccgtgggt ggagaatatg gaacaactgc aaatggagct
    18181 gatccggttg aaagagggac acgacgcttt ttcagcgcgg agagctacac taaagaagct
    18241 gttccacgag gcgacgcatg agtctgcatc gcgcagtttg ctggggcagc ttgggttgct
    18301 ggaatccgcg aaatcagatt agtttgactg ctgcatagtt tgtgcaataa gagcgatgca
    18361 gtgccggacg atggcttcag ggcgggcaca aaaacaatct tgaaaaacgg ttgctgctcc
    18421 tggttctgcg ctgaacaaac gagcaatggc ctcctgaatg gtggtcgcca gagtagcggg
    18481 ggcatttgag gcacaaacgc ggcggttatg gcctctgggc ggtgagcgcc acggatagcc
    18541 aatgcatttg gttttcaggg gcgataaagg ctcggtgcat tccggcggcg gcttgcgtgt
    18601 gcgcggggcg atagttgatc ggggcgatct caatagagtt ccaaccggct tgctcgaaag
    18661 cagcccggta atgcagtggg cggaggcggt tgggaatgcc cggaaagtaa aaccagcggt
    18721 acagccaatt cggataacgg taaatattgt tgggatcatg gtcgcggatc cagcgggaat
    18781 gggctttcag gtcgatttcg gcgacgattc gtgccccgct tttgcatata cgaaaaagtt
    18841 gatttgcgac ggccggaatg tcgtcaaagt gttcaaaggc cgcttggctg aataccaagt
    18901 ccattgagga ttctccgaag gcggtgcaga agtcaaaatc cttacggacg acatagcgca
    18961 gtttcgaggg ctggccattt ttccaatcaa gccatgcttg ccgaagagaa tccatattgg
    19021 cgcttgggac tcgaatatgg atggcctgga gcagttggtc gtagaaataa tccggggtgt
    19081 tatagacgag gggatggaca tcgcacgcgc tgtaacttgc tgcccccttg gcaagcaaat
    19141 agaggccaat ccccaaatct gaaccagggc ccagttccaa gatgtgtttc ccttcaatat
    19201 tgctgctgcc ggttgcttgt tgtaaccttg ccaaccattg gtccacaatg cgcagatcgt
    19261 aggcgatgct tctttcggtt tctgtgacgt tgaagggttt tggggtcacg tatccgcgca
    19321 ggcggttttt ggttttcgct agggccagaa aaattaggcc cgatacaaaa taaaaccagt
    19381 ggtgccggct ggggagggga tacggctgtg ctggggttgt tgcggctagc tgcatactgt
    19441 catacaacag gaaagccatt ttcgagtcga gcgtgaatcc gctaagcagc actggcccga
    19501 aggctcagtc cgtctgggct tctgggtggt tagtgtagtg gcggcgtaga gtgggtttgc
    19561 ggtggaaagc ggcttgcggt ggcggggcat tggcattagg ttggtcactc gaactaggac
    19621 gaggaggtca acgtgaatgt tgccattatc ggagccagca acaaacccga gcgttacagc
    19681 caccaagccg tgctgctgtt ggcggccaaa ggccacgccg tgtatcccat ccatccggca
    19741 ttgacggaga ttgagggaca ccccgtttat aagcgactgg ccgacattcc ggtgccggtg
    19801 cataccatca ccatgtatgt gggccccgcg ctctctgccg gaatggacga aaccttgatt
    19861 gccgccaagc cgcagcgggt gatttttaac cccggcacgg aaaatcccga gctggaaagc
    19921 aagttggcgg cggcgggcat ccaagtggtg caagcctgca cactggtgtt gttgcggacc
    19981 aatcagtttg acgcataatc agcgcccata aacgcgagct tgcctctgtt ggctcgcgtg
    20041 cttatggtgc gctctcctca acaaggaaag atatggctgc ttttacagaa tggctcaaaa
    20101 ccctggcgcc ggtaaatcaa gcgctggtgg gcactctttt cacgtggcta atgaccgcgc
    20161 ttggcgcggc ggccatcttt ttgacgcgtc agcccagccg aaagtttcag gatggcatgt
    20221 tgggctttgc ggccggcgta atgatcgcgg cgagcttctg gtcgctgctg gcaccggcca
    20281 ttgaaatgtc gggcgggagc tgggtgccgg cggtcgtggg atttttggtg ggcggcgtat
    20341 ttttgcggct ggtggatatt gcgttgccgc acttgcacgt gtatgcggag gacccagagg
    20401 gacgacgcag ccattggaag cggagcattc tgctggtgac ggccatcacg ctgcacaata
    20461 ttccggaagg gttggcggtg ggggtggcct ttggcgcggc ggccatcggc gaaagcacct
    20521 atagcgtggc gggcgcgatc gcgctgacga tcggcatcgg catccaaaac ctgccggagg
    20581 gtttggcggt ggccatgccg ctgcaacgca gcgggctaac acgttggaaa gggttcatgt
    20641 atggtcagtt ttcgggcatg gtggagccgc tggctggcgt gctgggcgca gtggccgtca
    20701 tttatgccca gcccattttg ccttatacgc tcgcctttgc ggccggggcc atgatctttg
    20761 tggtggtgga agatgccatc cctgaagcgc agcgtagcgg caacaatgat atcgccacgc
    20821 tcggcgcgat ggtcggattc gcgctgatga tgacgctgga cgtggcgctg ggttgaacag
    20881 gacgattttt tgcgccgtcc cgtttacggg tagagcaaat gccggaggga atagtccggc
    20941 cagggggggg cgggggtgcg ggtgccccat agttgggcat agaaatccgg gcgcgagccg
    21001 ggaaaacgcc agagcgccgg gccgagatat tcttccaaat gatggagcag cgtgaaggct
    21061 gcttgtgccg ggcggtacgc ggtgggatca gcaacttcaa acgctaggcc tgacagaatg
    21121 tgaccgtttt tgtcaggata ttcggctggc gtcacacgga ggccggggag tgggggcaat
    21181 tgcaggcggg cgaggtcggc gtgggcggag ccaagtcgtt ggaaggccag gggggtgccg
    21241 cgcgcgtggt ccattatggg cagcgcctcg aaaaacaccg tcaacggaaa gcacagcgcg
    21301 ttggccagcg cgatgatggc cggcgaggtg gtctgccagt tgggataaat ggccgccagc
    21361 ggcagcgacc gcgacagatt ccggcaaggt gcgatacgca aatcaagcgc ggcaagctgc
    21421 aactcatcgc gtaaaaagag cgccgtttcg gcgggtgtca tttggtagca aaacggggtg
    21481 gggatcatgc cgacaaacga agcacaggcg ggatcgagca ggggcccgtc gggcggcgtg
    21541 agcatcaagg ggtccgggcg gtcggccaca atgactgttt tgccggcgcg cgcgcaggtt
    21601 tccagcagca ggtgcagcgt ggagacatag gtataggctc ggcaggcgat ggtctgaatg
    21661 tcgaacacta taacatccac caactccagc atgggcgtcg tgggcgcgcg cgttttgcca
    21721 tagagcgaaa aaatcggaat gttccaatcg gcatgagccg tatcggcaat ggtttcgccg
    21781 gcgcccccac ggccaaagaa accgtgctcc ggaccaaaga gcgcggtgag gttgatccct
    21841 tggtcgcgca aaaatgccgc ggagtggcgg ccatcgcgca ctacgctggc cgggtgggcc
    21901 acgaggccca cgcgccgacc cgccagccat tccagcggca ggttttcaat gcccggagta
    21961 atgtcgtggc gattgctcat ggcgcgtacc gtagcaaagc cgcgccgcgc cgccgatgca
    22021 atttttcggc ccgccgacgg gtggacagag cgcgatggtt tggtcataat gaacccatga
    22081 caaccgcgct tattcgtcaa cattccgctt tgctccagca agctgcggag cagattgcca
    22141 ccgcgcgcca tctgaccgca ttcacggggg cggggatttc tgtggaaagc ggcatcccgc
    22201 cattccgcgg cccgggcggt atttggaaca agtatgatcc ggaagtgttg gacttgcatt
    22261 ttttccaacg cgatccggag caatcgtggg cagccattcg ggaaatgttc acttgctttc
    22321 tgggcacagc ggaccaaacg cctgtgttgc ccaatgccgc gcatcaggtg ttgggcgaat
    22381 gggaggcggc aggggtgttg aaagtggtca tcactcagaa tattgacggg ctccacgcag
    22441 cggctggcag ccgcaatttg gtggaatatc acggccattg tcgcagcatg acttgcctcg
    22501 attgtgccaa ggaactgccc cttgatcaca ccaccacacg tgagactgtg ccgcgctgcg
    22561 actgcggtgg tctgctcaag ccggacttca tctttttcgg cgagggaatt cccagtgaca
    22621 tggtggagcg gtccgaggcc gcggcccgtc aaaccgactg catgttgctg gtgggcacgt
    22681 ccggggcagt gtatccggca gcgtttctgc cggtgctggc caagcggttg ggcgcattca
    22741 tcatcgaaat caatccggag cccaccgagt tcaccagcgg tgtgacggac ctgcacctgc
    22801 cgctgccggc aggggcggcg ctgatggagc tcaatacgca ggtcaaccgt tttttacaca
    22861 ataataaatc gtaatccatg attgacgcgc gggggacggg ggcgtattgt tctgcgcaat
    22921 tccgccgcca tccgggcatg agagtcccgg ccgcggcagg tcaaacataa tacacggaga
    22981 tacgaaaatg gcgatcaagg ttggcatcaa tggattcggc cgcattggcc ggttggtttt
    23041 tcgcgcggcg atggaaaacc cgcaaattga agtggtgggc atcaacgatt tgtttgattc
    23101 caagcaactg gcgtacatgc tgaagtacga cagcatccac ggggcgttca aaggcacggt
    23161 ggaggccgct gataacgcgc tggtggtcaa tggcaagaac atccgcctga ccgcggagaa
    23221 ggacccggcg gcgctcaagt ggggcgatgt cggcgcggaa tacatttgcg aatccacggg
    23281 cttcttcctc gagaaggcca aggcggaagg gcacatcaag gccggcgcca agcgcgtggt
    23341 catgtcggcg ccttccaagg acgacacccc gatgttcgtt atgggcgtca accacaagag
    23401 ctacgccggc gaagcgtttg tctccaacgc ctcctgcacc acgaactgcc tggcgccagt
    23461 cgccaaggtg ctgcatgaca actggggcat cgccgaaggg ttgatgacca ccgtgcacgc
    23521 gaccactgcc acccaaaaga cagtggacgg cccgggcggc aagaaggact tccgcggtgg
    23581 gcgcgcggcg gcgggcaaca ttattccgtc gtccacgggc gcggcgaagg ccgtgggcaa
    23641 agtgattccg gcgttgaagg gcaagctgac cggcatggcc ttccgcgtgc cgacgctgaa
    23701 cgtctcggtg gtggacctga ccgtgcgcct ggagaaggcc gcgggcgcgg atgcggaatc
    23761 cgcctatgcc gccatcaagg ctgccatgaa gaaggcttcc gaaggtgaaa tgaagggcat
    23821 cctcggctac acgaccgaag atgtggtctc cacggacttc aaccatgacg cgcgcacatc
    23881 catctttgac gcgggcgcgg gcatcatgct caaccccacg tttgccaagg tggtttcgtg
    23941 gtacgacaac gagtgggctt attccaacaa gctcctcgac ctgatcgtgc acatgaacag
    24001 cgtcaagtag gcaatcccga ttgatgatga accacgcgga gagcggcaac cccgctctcc
    24061 gccgtcataa acaggagcgg caaatgaaca agaagacctt gcaggatgtg gagttgagcg
    24121 gcaagcgcgt ggtgatgcgc gtggacttca acgtgccttt gaagaatggc gtgattagcg
    24181 atgacacccg cattcaggcg gcgttgcctt cgatccggca cattctgggg cagggggcgc
    24241 ggctggtgct gatgagccac ctcggccggc ccaaaggcaa gggttacgag gcggagttca
    24301 gcctcaagcc tgtggccgag catttggcca aggtgctggg ccagccggtg gccttcgccg
    24361 cggattgcct tgccgccgat gctgaagtgg ctgcgttgca gccggggcag gtcctcctgc
    24421 tggagaatac ccgcttctac aaagaagaag aagccaaggt taaaaagacc gacgacatct
    24481 ccgaagagga ctacaaagcc caaaaggcgg cactgaaaga aaagcaaaag gccatggcga
    24541 aaaaactggc ctcctatggc gatgttttcg tgaacgatgc cttcggcgcg gcgcatcgcg
    24601 cgcacgcttc cacagcggtg atctgcaaat atattccggt cagtgtgagc gggttcctgc
    24661 tggaaaagga aattcgctat ctcggcaatg cggtgaatca tccggagcgt ccgtttgtgg
    24721 ccattttggg cggcgccaag gtcagcgaca aactggctgt ggtgaacaac ctgctggaca
    24781 aggtggacac gctgatcatt ggcggcggga tggcctatac cttcttgaag gcgcagggcc
    24841 acgatgttgg ccaatcgctg tgcgaattgg atcaactgga ttacgcccgc gacatgctgg
    24901 ccaaggccaa agccaacggc aaaaagctgc tgttgcccgt ggacaacgtg gcggccgaca
    24961 agtttgccgc cgatgccgca acccaggtgg tgggcaatga tattcccgcc ggctggatgg
    25021 cgctggatat cggccccaag accatcgcgg cctacgcggc ggcgatccaa ggcgccaaga
    25081 cggtggtttg gaacggcccg atgggctgct ttgaaatggc tccgttcgcc gccggcacgt
    25141 tcggcgtttg cgaggcggtg gccgccagcg gcgccaccag catcatcggc ggtggcgata
    25201 gtgtcagcgc cgtcaacaag agcggcttgg ccgccaagat gactcacatc agcactggcg
    25261 gcggcgccag cttggaatat ctcgaaggca aggaattgcc cggcgtagcc gccttgagcg
    25321 ataagtaacc cccaacccga acccgcaaag acaagaggac tcccatgcgt aaaaagattg
    25381 ttgccggcaa ctggaagatg aacatgacca cggcggaagc cgcggcgttg gttaacacgc
    25441 tcaaagccga tttggccaat ttccgcgagg tggaagtggt ggtgtgcccg ccctttaccg
    25501 cgctgagcac cgtgagcgaa ttggtacaca acacccacat tgatctgggc gcgcaaaaca
    25561 tgcactggga aaagaacggc gcgttcacgg gcgaaatctg tgccagcatg ctgcgcgaac
    25621 tgtattgcca ttatgtgatc ttgggtcaca gcgagcggcg gcagtatttc ggcgaaaccg
    25681 atgccatcgt caaccgcaag accaaggccg cgctggccgc cagtctgcac ccgattgtgt
    25741 gcgtgggcga aaccttgcag gaacgcgaag ccggcaaagt ggaagaagtc attaccaccc
    25801 aggtcaaggg cagcctcgcg ggtttgtcgg ccaaggaagt gttggagtcc gtgatcgcct
    25861 acgagccggt gtgggccatc ggcaccggca aaacggccac cagcgaccaa gcgcaggagg
    25921 tccatgcgtt gatccgcaac gtgctgacgg ggatgttcga tgccacggtg gcccagagcg
    25981 tgcggattca gtatggcggc agcgtcaagc ccgccaacgc caaggaactc ttcagcatgc
    26041 cggacattga tggtggcctg attggcggcg ccgcgctgga tgccaagtca ttcattgaca
    26101 ttgtgcgcgc ggcggtctaa atagtcgggt ctttcaggtt gcaaggagtt tccccatgag
    26161 cattctcatt actctgctgt tgttagtcga agtcatcacg gcgtttttgc tgatcgtggt
    26221 gattttggcc caaaagtcca aggatcaagg cttgggcatg gcgtttggga gtggcatggg
    26281 cgagagcctt ttcggcagcc gggcgggcaa cgtactcacg cggatgaccg tcaccctgat
    26341 cattgtcttc accattacga cggttgtgct ggggattctg ttctccggtg gacgtgaggc
    26401 atcgcgcagc ggttcggtga tggataccgt ggacatgagc cggcaggtgc cggcggcgcc
    26461 tgtggcggtg ccagctccga gtgccccgtt gttgccggaa gagcagccgt tggttgccac
    26521 ggaggaagtc ccttccgccg cgcaaacgct gcaagtggat gccgagggca acctgcaacc
    26581 ggtggaaggt gcttcaggtg aagctgtcgt cccggagacg gtggtagcgg ttgaggaaac
    26641 ggctgccgaa gcaggatctc cggcagaagt tgaagttgaa gccgttgtgg tggagacacc
    26701 tgctgaagct ccgacggcac ccgccgaagt cgaggaagcc gctccccaag actaatgaag
    26761 tccggctggt tgaccacatc cccgcgcacg gcctgcgcgg ggattttatt tgccctgctg
    26821 ctgctggctg ccggcggttg cggcagcgcg caccagaccg aacaagccac agacaccctg
    26881 cacctcacca ctatgcgcat tcgcggtttc gatccggccc actccgtgga tgaggcttcg
    26941 gtgcgcgcgg cgggcttggt ttacgagggc cttttgcaat atagctattg gacccgccca
    27001 taccgtgtgg agccactgct ggccgccacc atgccagagc tgtccgctga cggcaaaaca
    27061 tggcgcttcc tgctgcgatc cggcatctat tttgccgatg acccttgctt tgtagccagc
    27121 ggcggcaaag ggcgcgagct gatggccgcg gatgtggcgt attccattcg ccggatcgcc
    27181 gacaccaagg tcggctccag cg
//
LOCUS       DAOPNP010000018        26195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2386_length_26195_cov_8.117177, whole genome shotgun sequence.
ACCESSION   DAOPNP010000018 DAOPNP010000000
VERSION     DAOPNP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 26195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26195
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2386_length_26195_cov_8.117177"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1348)
                     /gene="rlmN"
                     /locus_tag="PKI64_02900"
     CDS             complement(308..1348)
                     /gene="rlmN"
                     /locus_tag="PKI64_02900"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOD99989.1"
                     /translation="MKTALQGLWPEELQALALAAGQPAFRAKQLWQWVQVRGATRWEQ
                     MGNLPAAWKSALAATHTPEPIRIIKETADAGGTRKWLGGLDDGECVETVLIPARDWTT
                     VCLSTQVGCRMGCAFCASAKGGFARSLSAGEIVGQFQLVMAATGRRPDNVVYMGMGEP
                     FDNYDEVLKSIRILNHPDGLNIGARRITISTSGVIPGIRRLAGEGLQVELSVSLHAVN
                     HELRRQLMPIENKYPLTELLAACRDYTAATKRFVTFEYTLIRGVNDSARDAEELAKHL
                     RPFSCRVNLIPLSPVPEFAGEAPTRVAIEQFQQTLAARGVSVTLRESKGKDVNAACGQ
                     LRRQHRLPQPSD"
     gene            complement(1470..2609)
                     /locus_tag="PKI64_02905"
     CDS             complement(1470..2609)
                     /locus_tag="PKI64_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOD99990.1"
                     /translation="MASPALLKRLARQHGTPLFVVDHAILRKNLATFRQQLPRVQPYF
                     AVKANSLPEIVRTFYRAGASFDVASIAEFRTVVDNIKDWPPKKQQDFIWDKIIYANPI
                     KDKRTLEELNPYKPLVTYDNLAEVHKIRQYAPDAGLILRLSVPNTGSVVELASKFGAP
                     PGEAVNLIEAAAAAGLTVEGLSFHVGSQCTNFQNYIQALALADSVMKEAWSRGHRQVR
                     ILDIGGGFPAPYDAHVRPFRDLARTLRAEFTRLFPPDMEILAEPGRFLVATACTLVAE
                     VIGKADRNGKRCYYINDGVYHTFSGILFDHCKYPVKAFRKGPTQLCAVFGPTCDALDT
                     ISLAEPLPELELGDLVYSPNIGAYSHASSTWFNGFPPAQVVHINQ"
     gene            complement(2614..3786)
                     /gene="speY"
                     /locus_tag="PKI64_02910"
     CDS             complement(2614..3786)
                     /gene="speY"
                     /locus_tag="PKI64_02910"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOD99991.1"
                     /translation="MSTIKLPKKFVSQYPHQFAAGKKRFLSGKRILPSPITGRESLPD
                     LINHTFLAYNAARLQEGCRLFTEKMLARDDVTVGMSLAGALTPAGLGSAAVVPLIKAG
                     FVDWIVSTGANLYHDMHHAMNLPLWKGSAAVRDPELRRLGVVRIYDIFLDYNDVLMRT
                     DDVLRQMLVQPEFQKEMGTAELHYLLGRYCAQFEEQTGRQDASILAAAYRAGVPCFCS
                     SPGDSTIGMNIAGVELCGNKLRINPSIDVNESTAIVLDAKQRRKKTGVVLVGGGSPKN
                     FTLQTEPQIQEVLRIPEVGHDYFLQFTDARPDTGGLSGATPSEAVSWGKVDPNQLPDA
                     VVAYLDSTVGLPLLTHYALAQRKPRKLKRLYDQRGAMMKRLTRVFFANNPDAPRQG"
     gene            complement(3809..4732)
                     /gene="speB"
                     /locus_tag="PKI64_02915"
     CDS             complement(3809..4732)
                     /gene="speB"
                     /locus_tag="PKI64_02915"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOD99992.1"
                     /translation="MTDLSRFQSPWNFLGLPAEATTPERARAWVLPIPYEGTTSYGAG
                     TREGPAAIIAASRQVEWFDREFNTEPALAIGIHTLPPLGLDHRSPEAMVNAIDEAVAG
                     ILTGSPAPELLVALGGEHSISGGIARGMVRAGIRDFVTVHVDAHADLREEYEGSRYSH
                     ACAARRIAEVSPLFQIGIRNLSAGEEHFRRSTDRVHTVFAEEALADRGWLNDLAAYVR
                     GRTVYLTVDLDGLDPSIMPAVGTPEPGGLSWADIMAVVRVVCREAASLPVCDVVELAP
                     LPGMKGPDFLAAKLVHNIIVQALYQRPSRQH"
     gene            complement(4822..6747)
                     /gene="speA"
                     /locus_tag="PKI64_02920"
     CDS             complement(4822..6747)
                     /gene="speA"
                     /locus_tag="PKI64_02920"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOD99993.1"
                     /translation="MSRGLTEALDGWDNIRSSELYGVEAWGNGYFHITEDGFAAVKLQ
                     NQQGPVSVKFHDIVRGLYQRGFSLPVLLRFSDLLAARIRAINEAFAKAIADSGYAGSY
                     RGVYPIKVNQQHQTVADVVRLGAPFHHGLEAGSKAELIAALGYLHDPAAYIICNGYKD
                     AEFIDLALFSLKMGLQTILVLEMPGELDLLLERAAKQNIRPRIGIRVKLASSVGGKWA
                     ESGGEGSVFGLTPPQVIAAVDRLRELGMLDCLELVHYHLGSQVPNIREIREAVREAAQ
                     YYTGLVKEGAKMGILDIGGGLAIDYDGSRCNCASSANYDVAEYAADVVEGVMDICDKS
                     GVPHPVLISESGRALVGYYSVLLVNVLNTRAIEVPDPPKPLPKPIPEPIRNLMEVAAN
                     LRTRNLGEFYNDAIFYREEIQKAFKHGDITLRQHAVADRLFWHILTRIKAEVPKLRFV
                     PDDMRDLEELMADIYYCNFSIFQSLPDSWAIDQLFPVMPIQRLNEKPTRLAVLSDITC
                     DCDGKIDHFVSMPDNRGALPVHGVGPDEDYILAIFLVGAYQETLGDLHNLFGDTNVAS
                     IHVDDAGEITYAEEFDGDSVGDVLSYVEYNPQGLLARFRELAEEAVRQKRISATERRE
                     ILDAYENGLRGYTYFET"
     gene            7000..7911
                     /locus_tag="PKI64_02925"
     CDS             7000..7911
                     /locus_tag="PKI64_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD99994.1"
                     /translation="MVDRPGAAAERTARMNSPAPAWPGIGELRQQIEAELEATLSNCP
                     QTEVMRAARYVTQGGGQRWRGALAVAAGAIFQPDALAATLPLAAALEIMHAASLVVDD
                     LPSMDHAQTRRGKPCVHLVFPPWVVDMLPAFLVNLAYHVAAQNTRVPTENRLRCLLLL
                     GEMGMNLAQGQELDLALTGQEATLAQVVNCHTLKSGSLFAAATAGGALLCGASDDDAA
                     TLRNAGMKLGLTYQIMDDLADSDNEQADVAGKSGIWTCCTPQEARALAAEQLAAALSL
                     LTPFGPPATALRALTAEIFSGPRHLPA"
     gene            complement(8203..8619)
                     /locus_tag="PKI64_02930"
     CDS             complement(8203..8619)
                     /locus_tag="PKI64_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99995.1"
                     /translation="MGRKTCMRTVFSIAALYDGLLGGSFLVAGERLFAAYKVAPPNHW
                     GYIQFPAALLLVFALMFAAIARRPWQNRNLIPYGMLLKVSYCSVIGWHWWHANIPGMW
                     VPFAVIDFVFLILFIAAWFATAHSNDACAPASPPTA"
     gene            complement(8633..9943)
                     /locus_tag="PKI64_02935"
     CDS             complement(8633..9943)
                     /locus_tag="PKI64_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD99996.1"
                     /translation="MNIGLFAMSGIRVCDAELLKLGLTLPGFVERSKTIASLPSLGLL
                     TLAGMTPRQHHVSYYEVPDVHQLEALPAGLDLAAISSYSAQMDEAYELARRLRAAGVA
                     VVLGGPHVTALPEEATACGATAVVGEGEATWPRVLADAARGALQPIYRAAPDDFNMAD
                     APLPAFELLAIEKYNRLTVQTSRGCPHRCEFCASSVILTERYKQKPAAKVLAEIARIK
                     EIWPQPFIEFADDNSFVHHAYWLDLLPALKGQGLRWFAETDLAVAQNPELLTLMRETG
                     CAQVLVGLESPVADGLQGLEQHSNWKARRLPQYREAIQTIQEHGITVNGCFVIGLDGH
                     GPAIFDQVFDFVDSSGLYEVQVTIQTPFPGTPLYARLAREGRLLEPRNWRKCTLFDIN
                     YEPANMTRQELHDGFHKLVVRLYGEEFTRRRRERFRQLRRGRKT"
     gene            complement(9940..10269)
                     /locus_tag="PKI64_02940"
     CDS             complement(9940..10269)
                     /locus_tag="PKI64_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99997.1"
                     /translation="MAGAVIGLFFHDPNWLGGYASWPRRMLRLGHIALFGIGILNLFF
                     GLTVRALGLTDGLTWISHLLLVAAVGMPAVCFLAAWKKPFRHLFFIPALAAMVGLIGF
                     AWRLVAG"
     gene            complement(10465..11223)
                     /locus_tag="PKI64_02945"
     CDS             complement(10465..11223)
                     /locus_tag="PKI64_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOD99998.1"
                     /translation="MMPPGINKASEPAAESPTAALSPVYGILQNASLVDYPGQLAAVF
                     FLTGCNFTCGFCHNAQLMGRADARGLPWAEVEQLSRRHRDNWVTGAVITGGEPTLHPG
                     VFRLVEQLREWGFLRKLDTNGSRPEVLARLLPLVDYVAMDVKFAPADYPARTGFQNIA
                     ALTESIRLIQAQARNYEFRTTVIEAWHSPEHIHAIGAWVQGAQRHVLQAFIPREELPD
                     PACRAMPETSAATLRAYAEILRGYVAQVDIRGEF"
     gene            complement(11237..11635)
                     /locus_tag="PKI64_02950"
     CDS             complement(11237..11635)
                     /locus_tag="PKI64_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD99999.1"
                     /translation="MAKTYQVHVFGKPACDKCHTLNGRLDDLLQEAEWADFEKVYHDL
                     ETENGLVEFCEAECLNPQRVPGFYVSKANPVTGVQEPLPNPTPGAMTPPVGASALYTW
                     VGIQTDYSATGRGVISPKMIEAVLRQAKSL"
     gene            complement(11639..14383)
                     /locus_tag="PKI64_02955"
     CDS             complement(11639..14383)
                     /locus_tag="PKI64_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOE00001.1"
                     /translation="MNKTEAIVFDGFRKRDGMVVPFRRDKIVLAIFRAADEVRRRDHV
                     EVGETMPEQMADLVIQQLSDPRSEYHVSPDEHGRRIPHIEDMQDLIEIVLAEQGYAPI
                     VAAYKRFRKHRQLARERIRVRSQQKAASGKPVDLTDASMLLVESVVHGHTLPWDRKEL
                     VDSLLRHTDLNEEVALNIAKTVENQVIASGMGSVDSTLVRELLNNELALRGYRHQLRD
                     LSVYGVSRQFVDRLMYAKSQENSNIVNNNPEAVQLALAELVLKQWALDTIFSADLKNA
                     HNTGALHLHDLGYPHRVYCSSHSVEYIKKYGLRGLNNLNNESAPASSAHVLTGHLHTF
                     VASMQANYAGALGLAYINIMFAPYLRGKSAKEIHQVAQGLIFGGAQNAFSRGGQTIFL
                     DFNIHTGVPAYLKDVPAVGPHGRYMLQRADGTIARLEEVIRDELDANGNRLMELYVTD
                     DDGTRRCVLREEPDPRHGQRYAADVAEALAARGERLLTYGDYEEEARELTRALLNVWE
                     AGDANGHVFEFPKCDFHVSAETFTDPAQLAIFQRACEVASRNGSVYFIFDRDEVTLSA
                     CCRLRTTIQDNYMLRHPESMRFCGFQNVTINIPQCMYRAARAGERTFEGLVAEVDKMM
                     DLCVKAHEQKRNRIAEMISGPGFPLWQIGKPACDGKPYVELDKATYIIGLIGVNDAVR
                     FLTGQEFHESEEAMRLGEKLVAHMFLRCKKLSVKHKMKLTLEESPAESAARRLAKADL
                     VYYRDEALQVYKGGSEDVAYYTNSVHLSADAPVSLVERIRKQARFHSLIESGAITHAF
                     IGEERPSPQAIETLMRETFFRTQSAQVTLSPEFTYCLDCGHNMRGLKERCTACGSAHV
                     EGETRVVGYFSKINNWNKSKREGELPARQRGIYAVEAASPVASQTMPAACPAPQPVEA
                     "
     gene            complement(14937..16247)
                     /gene="hisD"
                     /locus_tag="PKI64_02960"
     CDS             complement(14937..16247)
                     /gene="hisD"
                     /locus_tag="PKI64_02960"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOE00002.1"
                     /translation="MSLTIPIVKFSPHKELPELEKFLLRPAFDPKAEETARRVLADIR
                     ATGDRAVQKYTKEFNGLQLPVAKFAVPREEVLAASAEVDLVFKRSAAEIFTRIVRFGK
                     AGMRKDWSMASPKGGVLGERFTPLARVGAYIPGGAAPLVSTALMSLSLAKVAGVPEIV
                     ACTPCDKQGKVNPYLLHALEVAGATEIYRIGGIQAIGAMAYGTNTIRKVQKIVGPGGP
                     YVTAAKKLVYGDVDLDMVAGPSEIAVLADETAVPAHVAADLISQLEHGTGHEKALLVT
                     PYMSMASKVLAEMQRQIESLSRRAAIEVAAAAGAMFVVADTLDTGMDLINRFAPEHLE
                     IMTKEPRRWLQKVKNAGAIFVGPWTPESAGDFVAGPSHVLPTGGTAAFFSGLSVDSFR
                     KRSSVIAFTRADLLETLPLIEIMGQVEGLDGHARAGKIRFTPPS"
     gene            complement(16261..17205)
                     /locus_tag="PKI64_02965"
     CDS             complement(16261..17205)
                     /locus_tag="PKI64_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00003.1"
                     /translation="MPKLRSHSAAARRWQVFCRTGWRWAGVFVAFWLGGMALLFRLAP
                     TPAGPERPAPTCMTWWRATPPAAWDIRTLYTPAAFALSTPAGFTHALRREKVELLPPV
                     HMERPAYAYLLPEPVVESSADEWGWQLPRADDGAAAGGVLTKRRATRESARLLFSPGW
                     ESRLFAGIDLNFDDWTNAAWRARVELQFDERGTPRAVLGSAAAAFGQRVAAAGTQCPA
                     NGHGGVGAARPRHGSGAMIPLPLPDWLAVIFVSHLALIFLITLYYSVRRYRHVPRHRV
                     APFVFRCTGCGHVYLDHRNIPMAECEKCGTMNESTRSF"
     gene            complement(17216..17677)
                     /locus_tag="PKI64_02970"
     CDS             complement(17216..17677)
                     /locus_tag="PKI64_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE00004.1"
                     /translation="MRIPDQPIQLQFRSVRRRFKGRFRRAVGPIEVTALLNVMALVMM
                     FMFLSSRFILQPGVRVELPTAPFTGGAPYGARVLTLAQGGLIFYNDEKMELADLGAAL
                     AKSSDEPADSALLVEADEGTPYGTLMQVYALAAEAGIQNVVLATRPPPVAP"
     gene            complement(17674..18306)
                     /locus_tag="PKI64_02975"
     CDS             complement(17674..18306)
                     /locus_tag="PKI64_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE00005.1"
                     /translation="MWNVLVNGGLVIGLLLACGVFALLVFFERLFNLHRAQIPADDFL
                     KGIYNVLNRDNIVEAAALCEDAPGPVARLALAAVLNHDDSLEKIGRAIEDAGLEEIPR
                     MERGLGWLATLARITPLIGLLGTVLGMMQMLLAAKEAAPLVQSADLTGGLWYALAATA
                     LSLIIAILAYAGYNLLVSRVESILLDMERSASALLTFMRQWKRNREAPPA"
     gene            complement(18381..19238)
                     /locus_tag="PKI64_02980"
     CDS             complement(18381..19238)
                     /locus_tag="PKI64_02980"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOE00006.1"
                     /translation="MKTIGQLELVLPNVDAELFCDWVREVWGTEPVAFEKPGGAQTVV
                     KIFFATPAKARTHRRKIPARFAKSAAQAMPCHEKDWTTFWRHHFKIQDVGQRLRTVPI
                     WEKVPDQKRLNIRIEPGLSFGTGDHFTTRFCLEEVERACVEIKPRSMLDAGTGSGILA
                     IAAAKLGVPKVAGFDFDPVCVTQSRENAVLNRLPKGRIRFYQADILQTGWCRQPADLV
                     CANILTSVLLTAAADLWQATKKRLILTGIREFEGDSVADAFMRLGAREIRRDGDGEWC
                     GIVLARPIL"
     gene            19373..20017
                     /locus_tag="PKI64_02985"
     CDS             19373..20017
                     /locus_tag="PKI64_02985"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOE00007.1"
                     /translation="MKPIKSSRIWIVWVAILAFPLGGTAFRPAPPARLMTTAPAESAP
                     EMAAAETVEALDLAMAAADEELALRNAQLLVDAIIQIESNGNPRMVGRHGERGLMQIR
                     AGTWRDMTTRLFGRPLPFDRAFEPELNRRVGTAYLAYLQEMLLPQQAAWKSDERSLLL
                     AAYNAGPGALRQTHYNLAQMPRQTRDYVARASALHDVYTSDIAQADTRELAAAL"
     gene            complement(20036..20419)
                     /locus_tag="PKI64_02990"
     CDS             complement(20036..20419)
                     /locus_tag="PKI64_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00008.1"
                     /translation="MKWQNRILNKWLGVLALAAVCALGNGCHDDDDYDHDPPAGQGTI
                     VVDNISGGRVQIFVEGKYLDSIRSGHIRYFDLPPGLYRVVLDAEDTRRAWAADVDVLD
                     GRLTILQVRWCNGSFDCLDVAMYFD"
     gene            20615..21487
                     /gene="hisG"
                     /locus_tag="PKI64_02995"
     CDS             20615..21487
                     /gene="hisG"
                     /locus_tag="PKI64_02995"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOE00009.1"
                     /translation="MEQIKLGLPKGSLQESTFALMKKAGWTFKVGARSYMPSVNDPAI
                     SARLIRPQEIPRYVELGLLDAGLTGYDWIYENGADVVEVAELCYSKATSNPVRWVVAV
                     PNDSPIQTVKDLQGKRIATEAVGLTRRYLSEHGVTAEIEFSWGATEVKAPELVDAIVE
                     LTETGSSLRANQLRIVDTVLTSTTRLIANKDTWADAEKRAKIEQMAMLLTGALNAENK
                     VLLKMNAKKGDVPAVCGVLPALHAPTVNSLGDPSWVAIETVVDEAIVREIIPRLKMAG
                     ASGIIELALNKVVP"
     gene            21518..21607
                     /locus_tag="PKI64_03000"
     CDS             21518..21607
                     /locus_tag="PKI64_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOE00010.1"
                     /translation="MGSVKKKRRLKMAKHKRRKRLRADRHKKK"
     gene            21621..23564
                     /locus_tag="PKI64_03005"
     CDS             21621..23564
                     /locus_tag="PKI64_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE00011.1"
                     /translation="MRAASIVGICRSRFALGCLAIGLAVGCRSPQGPPPEPGGGLIAY
                     RDSLSPEQERTAKIHALFAQAVHHTHADEFAEAAELYRQALALSPTNEVLVVRLADTM
                     RVLHQNDEALALVEHFVEQNPAAETAMFWLALFYKQANETSRGIELLRRLTRLHPTLP
                     AGWLQLASLTARENDTNAVESILREGITKAKPPTPLRRQLLRLELIRYVGAKDDGERK
                     ASRERAVELLNQIIAAVPGDFEALAALGELLTEANDYKAASEIFETLTRRQPAVTENW
                     EKLAGLYLLLGERERAIATLEQVAHKLDLPPDAHYSLGNLYRDAKDYTNAAVQYKQAA
                     EASPHTPQMWVMWALMYSESEPDKTFEILNRALELNPKSAQIMEITAALYQQNAKFAE
                     AAKWLQKLHELVESEQATEPPSQYFYFNFALVCTHLRRTEEAAQWLQQAVEQDPAFLP
                     AYVQESFGATASYRQRALQVLRALAKRPTTFSAEAHLHLGNMYAFLERNKAAVAEYDK
                     VLAIVAADPLQEGILNAQFYFWYGVVLDETGQTERAIPMFERCLEKDSSFANAHNYLA
                     YTWAVRNERLDEALRHIQIALAMEPENAAFLDTLGWVYFKQGQYADALKLLEKANELR
                     PHDPEIEDHLTQTREKLAAPAGK"
     gene            23576..>25014
                     /locus_tag="PKI64_03010"
     CDS             23576..>25014
                     /locus_tag="PKI64_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOE00012.1"
                     /translation="MRAPRKAFILAAGLGTRLRPLTLTTPKPMLPLWDRPMLARTLAM
                     LADWGVQQVLINLHHAPGPILNYLASQPFPQLRITAVFEPTILGTGGALRNARWFLDE
                     PCWLLNADIVAHVSPRPLLRVWHKERPLAACWLTDAAGPKTVRCADGWIQDFHRPQDG
                     QATFCGLQLLDQRILPFIAPEGFDTIIAAYQRAQQQGHRIAGVCVPGSFWADIGTPAQ
                     YVQAHRDTAGLFGRAGEPFNLPAAATFSSPEQKCLRITVDHPVTVLPPRGSNREFFKV
                     PDLPGISGPALVIRWSPERPENKLYAGHTVFLQKLGIPVPRLLLNAPRARLLAMEFIG
                     PDTLESRLPRLTPTQALRLYRQVLTITLRLHTRGLAVARRIRRPLMPGFTPQLYAWEH
                     DYFIQHVMRELLRASASRCAKVADELRTVANLLAASPQALIHRDWQSSNILLRGNGPV
                     MIDYQGMRRGPIAYDLASLLCDPYANLPAA"
     assembly_gap    25015..25114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25115..>26195)
                     /locus_tag="PKI64_03015"
     CDS             complement(<25115..>26195)
                     /locus_tag="PKI64_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE00013.1"
                     /translation="LRLMLCAPTGRAAKRMTELSGMEAKTIHRMLAYEPGSGRFRYNA
                     KSPLETDVLIVDETSMIDIQLAVHLFRAVPRHGLLILVGDVDQLPSVGPGCVLRDIIE
                     SNTIPVFRLTEIFRQSDGSQIVRNAHRVNSGQMPVLPGKDEALAGHSDFYFVSAETPA
                     HGVELIRKLVCEAIPRRFRLQPLRDVQILTPMQKGELGARNLNAAMQAALNPQGREVE
                     RYGWKYRERDRVMQTENNYDKDVFNGDVGTIVKVDAAEQEVLVRYDDRQVAYDFQELD
                     ELTPAYAVTVHKSQGSEYPCVVLPLHTQHYVMLQRNLLYTGITRGKQLVVLVGTTKAV
                     GLAVRNAEAGRRNTTLRRRLREAQR"
BASE COUNT         5197 a         8258 c         7673 g         4967 t
ORIGIN      
        1 tcccgcccta caaaagcagc cggttggatt gtagggctgg aacggtcgca gaccttccag
       61 ccgttcccgg cgggaaggtt tcgtccctca accattcccg ccctacaaaa gcagccggtt
      121 ggattgtagg gctggaacgg tcgcagacct gccagccgct cccggcggga agggttcatc
      181 cctcaaccat tcccgcccta caaaagcccg acaaaatcag cgaaattgtt gggtgtccca
      241 acgcggaact caggtccacc cgcacgccat agcacggcgc aaaagcgcga cgggtttcgg
      301 ctgccgatca gtcggatggt tgcggcaaac ggtgctgccg gcgaagttgc ccgcaggcgg
      361 cattcacatc tttgcccttg gattcacgca gcgtgacgga taccccgcgg gcggccaagg
      421 tctgctgaaa ttgctcaatg gccacgcgcg ttggcgcttc gccggcaaac tccggaaccg
      481 ggctcaatgg gatcaaattc acgcggcagg aaaacggtcg caaatgcttg gccaattcct
      541 ccgcgtcgcg cgcggagtcg ttgactccgc gaatcaacgt atattcaaat gtgacgaagc
      601 gtttggttgc cgcggtgtag tcccggcagg cggccagcag ctcagtcaac ggatatttat
      661 tttcgatggg catcagttgc cggcgcagtt catggttgac cgcgtggagc gagactgaca
      721 actccacttg caacccctcg cccgccagcc gccgaatgcc cggaatcacg ccgctggtgc
      781 tgatggtgat gcggcgcgcg ccgatgttca gcccgtccgg gtgattcaaa atgcgaatgg
      841 atttgagtac ttcgtcatag ttgtcgaagg gttcgcccat gcccatgtac accacattgt
      901 ccggccggcg gcccgtagct gccatgacca gttgaaactg gccgacgatt tcgcctgctg
      961 acagactgcg cgcaaagccg cccttggcgc tggcgcaaaa cgcgcagccc atccggcagc
     1021 ccacctgcgt ggaaaggcaa acggtagtcc aatcgcgggc gggaatcagc acggtttcga
     1081 cgcattcgcc atcgtccagc ccgcccagcc atttgcgcgt gccgccggca tccgccgttt
     1141 ccttgatgat gcggatcggt tcgggcgtgt gcgtggccgc cagcgcggat ttccaagccg
     1201 ccggcaagtt acccatctgc tcccaccggg tggccccgcg cacctggacc cattgccaaa
     1261 gctgcttggc ccgaaacgcc ggctggcccg ccgccagtgc cagcgcttgc agctcttcgg
     1321 gccataatcc ttgcagggcg gttttcattt ggcgggccag catagcgcat ccgcacccgc
     1381 cgcgccattt ttcttcccgt gctgaagatc acggcaggcg cggcgatgga gccggatgat
     1441 ctccccgcca ccaagtttcc gcaaatgcgc tactgattga tgtgcacgac ctgcgcggga
     1501 gggaagccgt tgaaccaggt ggatgaagca tggctgtagg cgccgatgtt cgggctgtac
     1561 accagatcgc ccaattccag ttccggcagc ggctcggcga ggctgatcgt gtccaacgcg
     1621 tcgcaggtgg gcccgaaaac cgcgcacaat tgcgtgggtc ccttccggaa ggccttcacc
     1681 gggtacttgc aatggtcgaa gaggatgccc gaaaacgtat ggtacacgcc atcattgatg
     1741 tagtagcagc gtttgccgtt gcggtcggcc ttgccgatga cttccgccac cagagtgcaa
     1801 gcggtggcca ccaggaagcg gcccggctcg gccaggattt ccatgtcggg cgggaacagc
     1861 cgcgtgaact ccgcgcgcag cgtccgggcc aagtcccgga atggccgcac atgcgcatcg
     1921 tatggcgccg ggaacccgcc gccaatgtcg agaatccgca cctgccggtg cccacgcgac
     1981 catgcctctt tcatcacgct atcggcgagg gcaagggctt ggatgtagtt ttggaaattc
     2041 gtgcattggc tgccgacatg aaagctaagg ccttccactg tcaggccggc agcggccgcc
     2101 gcctcgatga ggttgacagc ctcgcccggg ggggcgccga atttcgaggc cagttccacc
     2161 accgagccgg tattgggcac gctgaggcgc agaatcaagc cggcgtcggg agcgtattgg
     2221 cggattttgt gcacctccgc caaattatcg tacgtcacca atggtttgta cgggttcagc
     2281 tcctcgagcg tgcgcttgtc cttgattgga ttggcataaa tgattttgtc ccagatgaag
     2341 tcctgctgtt ttttgggcgg ccagtccttg atgttgtcca ccacggtgcg gaattccgca
     2401 atggaggcga catcgaagct cgcgccggcg cgatagaacg tgcggacgat ctcgggcaag
     2461 gaattcgcct tgacggcgaa ataaggctgt acgcgcggca gttgttggcg aaacgtcgcc
     2521 agattcttgc gcaagatggc gtgatccacc acaaagagcg gcgtgccgtg ctgccgggcc
     2581 aggcgtttga gcaaagcggg cgaggccatg ggttcaacct tggcgcgggg catcgggatt
     2641 attggcaaaa aagacgcgcg tcagccgctt catcatggcg ccccgctggt cgtagagccg
     2701 tttcagcttg cgcggcttcc gttgcgccag cgcgtaatgc gtcagcaagg gcaggccgac
     2761 ggtggagtcg agataggcca cgacggcatc gggtagttgg ttcgggtcca ccttgcccca
     2821 actgacggcc tcggaaggcg tcgccccgga gaggccgccg gtatcgggcc gcgcgtcggt
     2881 gaattgcaga aaatagtcat gaccgacctc gggaatgcgg agcacttcct ggatttgcgg
     2941 ttcggtctgg agcgtaaagt tcttgggcga gccgccgccc accaacacca cgccggtttt
     3001 tttgcggcgc tgcttggcat ccagcacgat ggccgtgctt tcgttgacgt caatgctggg
     3061 gttgatccgc agtttgttgc cgcacaactc cacgccggcg atgttcatgc cgatggtcga
     3121 atcgccgggc gatgaacaaa agcaaggcac gccggcgcgg taggccgcgg ccaggatgga
     3181 agcgtcctgc cggccggtct gctcctcgaa ttgcgcgcaa tagcggccaa gcagatagtg
     3241 caattccgct gtgcccatct ctttttgaaa ttccggctgc acaagcatct ggcgtagcac
     3301 gtcgtcggtg cgcatcaaca cgtcgttata gtcgaggaag atgtcgtaaa tgcgcacaac
     3361 gcccaagcgg cgcagttcgg gatcccggac ggcggccgaa cccttccaga ggggcaggtt
     3421 catcgcgtgg tgcatgtcgt ggtagagatt ggcgccagtg gatacgatcc aatccacgaa
     3481 gccggcctta atcagcggca ccacggccgc cgaacccaga ccagccggag tcagcgcgcc
     3541 ggccaaactc atgccgaccg tcacgtcatc gcgggccagc atcttctcgg tgaacaggcg
     3601 gcagccttcc tgcagtcgcg cggcattata ggccagaaac gtgtggttga tcaggtcggg
     3661 cagcgactcc cggccggtaa tcggcgaagg cagaatgcgc ttgccgctta ggaatcgctt
     3721 cttgcccgcc gcaaactggt gcgggtattg gctgacgaat tttttgggca atttgatcgt
     3781 gctcattttg ggttctcctt ggcaggtttc aatgttggcg ggacggtcgc tgatataagg
     3841 cttggacgat gatgttgtgc accaatttgg cggcgaggaa atccggcccc ttcatacccg
     3901 ggagcggcgc cagttccacc acatcgcaga ccggcaaaga tgcggcctcg cggcaaacca
     3961 cgcgcaccac ggccatgata tccgcccaag acaatccccc aggctcgggc gtgccgactg
     4021 ccggcatgat gctcgggtca aggccatcca agtccaccgt cagatagacc gtccgcccgc
     4081 gcacgtaagc ggccagatca ttcaaccagc cgcggtccgc caacgcctcc tccgcgaaga
     4141 cggtatgcac gcggtcggtg ctgcggcgga aatgttcttc gccggccgac aaattacgaa
     4201 tgccgatctg gaaaagcggg gagacttccg cgatgcgccg cgcggcgcag gcgtggctgt
     4261 agcggctgcc ttcgtattcc tcccgcaaat cggcatgcgc atccacatgc accgtcacaa
     4321 aatcccggat gccggcccgg accatgccgc gggcgatgcc gcctgaaatc gagtgttcgc
     4381 cacccagcgc caccaataac tccggcgccg gggagccggt gagaattccc gccacggctt
     4441 cgtcaatggc attcaccatg gcttccggcg aacgatggtc cagccccagc ggtggcagcg
     4501 tatggatgcc aatggccagt gccggctcgg tgttgaattc gcggtcgaac cactccactt
     4561 ggcgggaggc cgcgatgatg gcggccggcc cctcgcgcgt cccggcgccg taggaggtgg
     4621 tgccctcgta gggaatgggc aaaacccagg cgcgggcccg ctcgggggta gtcgcctcgg
     4681 ccggcaatcc caggaaattc catggcgatt ggaaacggct caaatccgtc atgtcggcgc
     4741 tccgtggttt gggtggtggg ggttcatggt ttggcgaacc ggcccgcgcg ccggcgaaaa
     4801 ccgccggcgc cggcaaccgg ttcaggtttc aaaataggtg tagccgcgca atccattttc
     4861 ataggcatcg agaatttccc gccgttccgt cgcggaaatc cgtttttggc gaacggcttc
     4921 ctcagccagc tcgcggaacc gcgccaacaa cccctgcgga ttatattcca catagctgag
     4981 tacatcgccg acgctatccc catcgaattc ctccgcgtag gtaatctcgc cggcgtcatc
     5041 cacatggatg ctggccacat tggtatcgcc aaacaagttg tgcaagtcac ccagggtctc
     5101 ctgataggca cccaccagga aaatcgccag aatgtagtct tcatccgggc caacgccatg
     5161 caccggcagc gcgccgcggt tgtcgggcat gctgacaaag tggtcaatct tgccgtcaca
     5221 gtcgcaggta atgtccgaga gcaccgccag ccgggtgggt ttttcgttaa gccgctggat
     5281 gggcatcacc gggaaaagct ggtcaatggc ccaactgtca ggcagcgatt ggaagatact
     5341 gaagttgcaa tagtagatgt cggccataag ttcttcgaga tcgcgcatgt catcgggcac
     5401 aaagcgcaac ttgggcacct cggctttgat gcgcgtgaga atgtgccaga aaagacggtc
     5461 ggccaccgcg tgctggcgca aggtgatgtc gccatgtttg aaggccttct ggatttcctc
     5521 gcggtaaaaa atggcgtcgt tgtagaattc cccgaggttg cgcgtgcgca gattggcggc
     5581 cacttccatc aggttgcgga tgggttccgg aatcggtttg ggcaacggct ttgggggatc
     5641 gggcacttca atggcacggg tgttgagcac gttgaccagc agcaccgaat aatagccaac
     5701 caacgcgcgg ccggattcgc tgatcaagac cggatgcggc acgccggact tgtcgcaaat
     5761 gtccatcacg ccttcgacga catcggccgc atattcggcc acatcgtagt tggcactgct
     5821 ggcgcaattg caccgggaac cgtcataatc aatggcgagg ccgccgccga tatcgagaat
     5881 gcccatcttg gcgccttcct tcaccaaacc ggtgtagtat tgggcggctt cgcgcacggc
     5941 ctcgcgaatt tcccggatgt tgggcacctg cgagccgaga tggtaatgca ccaattcgag
     6001 gcaatcgagc attcccaact cgcgcagccg gtccaccgcg gcgatcactt gcggcggcgt
     6061 caggccgaag acgctcccct cgccccctga ttccgcccac ttgccgccaa cggaactagc
     6121 cagcttgacg cgaatgccaa tgcggggccg gatgttttgt ttggccgcgc gctccaacag
     6181 cagatccaat tcccccggca tctccagcac cagaatcgtc tgcaaaccca tcttcagcga
     6241 gaacagcgcc agatcaatga attccgcgtc cttatagccg ttgcaaatga tgtaggccgc
     6301 gggatcatgc aagtaaccca gcgcggcgat cagttccgcc ttcgatcctg cttccaagcc
     6361 atggtgaaat ggcgcgccca gccggaccac atccgccact gtttgatgtt gttgattgac
     6421 cttgatggga tacacgccgc gataactccc ggcataaccg gagtcggcaa tcgctttggc
     6481 aaaggcctcg ttgatggcac gaatgcgcgc cgccagcaaa tcactgaatc gcagcaacac
     6541 cggcagactg aacccacgtt ggtacagccc ccgcacaatg tcatgaaact tgacgctgac
     6601 cggtccctgt tggttttgca gtttgaccgc cgcaaaacca tcctcggtaa tgtgaaaata
     6661 gccgttgccc cacgcttcta cgccatacaa ttcgctgctg cgaatgttgt cccagccatc
     6721 caacgcttcc gtcaatccgc gcgacatctt gttccgttct cctcgatcac ggtttatttg
     6781 gtcagattcc acaggccctg tggttcatgt ctgggcgaaa aagtgcggca aacatttcgc
     6841 caacgcaata gcaaatcaat gccaaacgta ttttttttta gggcggagtt gtcgggcctg
     6901 aggggttgca gtacctttcg ctggtgacac cggctacaaa gcacactata tttccttcat
     6961 tcgcaacatg atgagcctac tacacgcatg gaatcggtca tggttgaccg ccccggagcc
     7021 gcagccgaac ggacggcccg catgaacagc cctgcgcccg cctggcccgg cattggtgaa
     7081 ctccggcagc agatcgaagc tgagttggaa gccaccttgt ccaactgccc gcaaacggaa
     7141 gtcatgcgtg cggcgcgtta cgtgacgcag ggcggcggcc aacgctggcg gggggcgctg
     7201 gcggtggccg cgggcgcaat ctttcaaccc gatgccctcg ccgccacgtt gccattggcc
     7261 gccgcgctgg aaatcatgca cgccgcctcg ctggtggtgg acgatttgcc ttccatggat
     7321 catgcccaaa cccgtcgcgg caagccctgc gtgcatttgg tgtttccgcc gtgggtcgtg
     7381 gatatgctcc ccgcgtttct ggtaaacttg gcctatcatg tcgccgccca aaacacccgg
     7441 gttcctacgg agaaccggct gcgctgcctc ctcctactgg gcgaaatggg aatgaacttg
     7501 gcacagggac aggaactcga tctcgcactg accggccaag aggccacact cgcgcaagtg
     7561 gtcaactgcc ataccctaaa gagcggttcg ctctttgccg cagccaccgc cggcggagca
     7621 ttgctatgtg gcgccagcga tgatgatgcc gcaacattac gtaatgccgg aatgaaattg
     7681 ggcctcacgt accaaatcat ggacgacctc gcggatagcg ataacgaaca agcggatgtc
     7741 gccggcaaaa gcggcatttg gacctgctgc accccgcagg aagcccgcgc actggccgcc
     7801 gagcaactgg ctgcggcgct atccctgctg actccttttg ggccgcccgc gacagcgcta
     7861 cgcgccctga ccgccgaaat cttttccggc ccccgccacc tccccgccta gcccctgccg
     7921 aagaagcaga gttcagaggt caaaggtcag acgggaacaa aaataaaaat attccaaacc
     7981 caaaaaatct ccccgcccgt tgaatatttt ttcacagtgt ggaaaagttt tttccacagc
     8041 gtggaacaat cgggccggct cattgccgat tgaagtagtg ttttttgggc aacaactatc
     8101 atgcgggacg gcactgaacg gtaaaaccca ataattatca atcagattac gaagtaaaaa
     8161 agtgagtggt tcactgcgtc tttttaccct tccgtggcga ggtcaggcgg tgggcgggga
     8221 tgcgggtgca caggcatcgt tgctatgggc ggtggcaaac caagcggcaa tgaaaagaat
     8281 caggaagaca aaatcaatga ctgcgaaggg cacccacatt ccgggaatgt tggcgtgcca
     8341 ccaatgccag ccaataacgc tgcaatagga cactttcaac aacatcccat acgggatcaa
     8401 gttgcggttc tgccagggcc ggcgcgcaat ggcggcaaac atcagcgcaa acaccagcag
     8461 cagcgcggcc gggaattgga tgtagcccca gtgattgggc ggcgcgactt tgtaggccgc
     8521 aaacaggcgc tccccagcca ccaggaatga gccgcccaac aagccgtcat acaacgctgc
     8581 gatgctgaac accgtgcgca tacaggtttt tctgcccatg attttcctcc atttaggttt
     8641 tgcgcccgcg ccgcagttgg cggaaccgct cccggcggcg acgggtaaac tcttcaccgt
     8701 ataaacgtac caccaacttg tggaaaccgt catgcaattc ttggcgggtc atgttggcgg
     8761 gttcgtaatt gatgtcgaac aacgtgcact tgcgccagtt gcgcggctcc agcaggcgac
     8821 cctcgcgcgc gaggcgggcg taaagcgggg tgccgggaaa tggtgtttgg atggtcactt
     8881 gcacctcgta gagcccggac gagtccacga aatcaaaaac ttggtcgaaa atagccggcc
     8941 cgtggccatc gaggccaatc acgaagcagc cattgaccgt aatgccgtgc tcctgaatgg
     9001 tctgaatggc ttcgcgatac tgcggcaggc ggcgcgcttt ccagttggag tgctgttcaa
     9061 gaccttgcag gccgtcggcc acgggacttt ccaagccaac cagcacttgc gcgcagccgg
     9121 tttcgcgcat cagcgtaagc aactccggat tctgggccac ggccaaatcg gtttctgcga
     9181 accagcggag cccctgcccc ttcaatgccg gcaacaagtc cagccagtag gcgtgatgga
     9241 caaagctgtt gtcatccgcg aactcaatga acggctgcgg ccagatttcc ttgattcgcg
     9301 caatctctgc cagcaccttg gccgcaggtt tttgtttgta gcgctcggtc aggatcaccg
     9361 agctggcgca aaactcgcag cggtgtgggc aaccgcggct ggtttgcacc gtcagacggt
     9421 tatatttttc gatggccagc aattcaaacg cgggcagcgg cgcatcggcc atgttgaaat
     9481 cgtccggcgc ggcgcgataa atcggttgca gcgcgccgcg tgcggcgtcc gccagcacgc
     9541 gcggccatgt ggcttcgccc tcgcccacca cggcggtggc gccgcaggcc gtagcttctt
     9601 cggggagcgc cgtcacgtgc ggcccgccca gcactacggc cacccccgcc gcacgcaacc
     9661 gccgcgccaa ttcataggcc tcgtccattt gcgcgctata cgaagaaatc gccgccaaat
     9721 ccaagcccgc gggcaaggcc tccagttggt gaacatccgg cacttcgtaa tagctgacat
     9781 ggtgctgtcg gggcgtcatc ccggccagtg tcagcagtcc caaactgggc aatgaagcga
     9841 tggtcttgct gcgctccaca aaaccgggca gcgtaagccc cagtttgagc aactccgcgt
     9901 cgcagacgcg gatgccactc atggcaaaca ggccgatatt catcctgcca ccaaccgcca
     9961 cgcgaaacca atcaggccga ccattgcggc cagcgccgga ataaagaata aatggcgaaa
    10021 aggctttttc catgccgcca gaaagcacac cgccggcatg ccgaccgccg ccaccaacag
    10081 caaatgagaa atccacgtaa gaccatccgt caggcccaat gcccgaacag tcagaccaaa
    10141 gaacaaatta agtattccaa taccaaataa ggcgatgtgc cccagccgca acatgcggcg
    10201 tggccatgag gcgtagccac ccagccagtt tggatcatgg aaaaagagac cgatgaccgc
    10261 ccccgccacg cagcccagcg caaatcctac ccacccggcc tgaatattga tcatcatggc
    10321 gctgcgctgc ctcctgttca gcttgtgggg cgagtatagc ccgctggcgg gtgatgtcaa
    10381 agcatggaac ggggcacgcc tgagttccgc attcgtacgc ccggttattt cactgatttt
    10441 gtgtggccca accggaagcg caggtcagaa ctcgccgcgg atatccacct gggcgacgta
    10501 gccgcgcaaa atctcggcat aggcccgcag agtggcggcg ctggtttccg gcatggcgcg
    10561 gcaagccgga tcgggtagtt cctcccgcgg gatgaacgct tgcagcacgt ggcgctgcgc
    10621 gccctgcacc cacgcgccaa tagcgtgtat atgttcgggg ctgtgccaag cttcgatcac
    10681 cgtggtgcgg aattcataat tcctggcctg cgcctgaatc aggcggatgg attccgttag
    10741 cgcggcgatg ttctgaaagc cggtgcgcgc gggatagtcc gccggcgcga atttgacatc
    10801 catcgccaca taatccacca gcggcagcag ccgggccaag acctctggtc gcgaaccgtt
    10861 ggtgtccaac ttgcgcagga agccccattc acgtagctgc tccaccagcc ggaaaactcc
    10921 gggatgcagc gtcggctccc cacccgtgat gacggcgccg gtcacccagt tatcccgatg
    10981 tctgcggctg agttgttcca cttcggccca cggcagcccg cgcgcatccg cgcgacccat
    11041 caattgagcg ttatggcaaa aaccgcaggt gaaattgcaa cccgtcagaa aaaaaacagc
    11101 ggccagttgg ccgggataat ccaccagcga ggcgttttgt aaaataccat agacgggtga
    11161 caacgcggcg gtcggtgatt cggcggcggg ctcagatgcc ttgttgattc ccggcggcat
    11221 catggcgggc aacggctcaa agactcttgg cctgccgcaa gaccgcttcg atcatcttgg
    11281 gcgaaatcac gccgcgaccg gtcgcggaat aatcggtttg aatccccacc cacgtgtaaa
    11341 gcgcgctggc gccaacaggc ggcgtcatag cgccgggcgt cggattgggc aatggctctt
    11401 ggacacccgt caccggattg gccttggaca cgtaaaaacc cgggacacgc tggggattca
    11461 agcactcggc ttcgcagaac tccacgaggc cattttcggt ttcgaggtcg tgatagactt
    11521 tttcaaaatc cgcccattcg gcttcttgga gcaaatcatc caagcggcca ttaagcgtgt
    11581 ggcacttgtc gcaagccggt ttgccaaaca catgcacttg gtaggtcttg gccatggttc
    11641 aagcctccac cggttgcggc gcggggcaag ccgcgggcat tgtttgggac gcgacgggcg
    11701 acgcggcttc caccgcatag atgccgcgtt gtcgtgcggg cagttcgccc tcgcgcttgc
    11761 tcttgttcca gttgttgatc ttgctgaagt agcccaccac gcgggtttcg ccttccacgt
    11821 gcgcggagcc gcacgcggtg cagcgctcct tcaggccgcg catgttgtgg ccgcaatcga
    11881 ggcaataggt gaactccggc gagagcgtca cttgggctga ctgcgtgcgg aagaaagtct
    11941 cgcgcatcag ggtctcgatg gcctgcggcg aaggccgttc ttcgccaatg aacgcgtgcg
    12001 tgatggcgcc gctttcaatg aggctgtgga aacgcgcctg cttgcggatg cgctccacca
    12061 gcgataccgg ggcatcggcg gacagatgca cactgttggt gtagtacgcc acatcttcgc
    12121 tgccaccttt atatacttgc agagcctcgt cgcggtaata caccaggtcg gccttggcca
    12181 gccggcgcgc cgcgctctcg gcgggcgact cttccaacgt cagcttcatc ttgtgtttca
    12241 cggaaagttt cttgcagcgc aggaacatgt gggccacgag cttttcgccc aaacgcatgg
    12301 cttcttcgct ttcgtggaac tcctggcccg tcaggaagcg cacggcatca ttcacgccaa
    12361 tgaggccgat gatgtaggtg gccttgtcga gctccacata cggcttgcca tcgcaagccg
    12421 gcttgccgat ttgccacagc gggaagcccg ggccgctgat catttccgcg atgcggttgc
    12481 gcttttgctc gtgcgccttc acgcagaggt ccatcatctt gtccacctcg gccaccagcc
    12541 cctcaaacgt gcgctcgccg gcgcgtgccg cgcggtacat gcactgcggg atgttgatgg
    12601 tcacattctg gaatccgcaa aaccgcatgc tttcggggtg ccgcagcatg tagttgtcct
    12661 ggatggtcgt gcgcagccga caacaggcgg acagtgtcac ctcgtcgcgg tcgaaaatga
    12721 aatagaccga gccattgcgg ctggccactt cacaagcgcg ctggaagatg gccaactgcg
    12781 ccgggtcggt gaaggtttcg gcgctgacgt ggaaatcgca cttggggaac tcaaatacgt
    12841 ggccgttggc gtcgccggcc tcccagacat tgaggagggc gcgggtcagt tcgcgggcct
    12901 cttcttcgta atcgccatag gtcagcaggc gttcgccacg ggcggcaagc gcctcggcca
    12961 catcggcggc atagcgctgg ccgtggcggg gatcgggctc ttcgcgcagg acgcagcggc
    13021 gcgtgccgtc gtcatccgtc acatacagct ccatcaaccg gttgccgttg gcatccagct
    13081 cgtcgcgaat gacctcctcc agccgcgcga tggtgccgtc ggcgcgttgc agcatgtagc
    13141 gcccgtgcgg gccaaccgcc ggcacatcct tcaagtaagc cggcacgccg gtgtgaatgt
    13201 tgaagtcgag gaagatggtc tgcccaccgc gtgagaacgc attttgcgcc ccgccaaaaa
    13261 tcaggccctg cgccacttga tggatttcct tggcgctctt gccgcgcagg tacggcgcaa
    13321 acatgatgtt gatgtaagcc agcccgaggg cgcccgcata gttcgcctgc atggaagcca
    13381 caaacgtatg aaggtggcca gtcagcacgt gcgcgctgga ggccggtgcg ctttcgttgt
    13441 tcaggttgtt cagcccgcgt aggccgtact tcttgatgta ttccaccgag tgcgacgagc
    13501 aatacacccg gtgcggataa cccagatcgt gcaagtgcag tgcgcccgtg ttgtgcgcgt
    13561 ttttcaggtc ggcggaaaag atagtgtcca gcgcccactg cttcaacacc agctccgcaa
    13621 gggccaactg cactgcctcg gggttgttgt tgacgatgtt gctgttttcc tggctcttgg
    13681 catacatcag ccggtccaca aactgccgcg aaacgccata gaccgagagg tcgcgcaact
    13741 gatgccggta accgcgcagg gcaagctcgt tgttgagcaa ctcgcgcacc agcgtgctat
    13801 ccacgctgcc cataccactg gcgatcactt ggttctcaac ggtcttggca atgttcagcg
    13861 ccacctcttc attcaaatcg gtgtgccgca gcaacgagtc caccagctcc ttgcgatccc
    13921 acggcagcgt gtggccgtgc accaccgact ccaccagcaa catactggcg tcagtcaaat
    13981 ccacgggctt gccactggcg gccttttgct ggctgcgcac ccggatgcgc tcgcgtgcca
    14041 attggcgatg cttgcggaag cgtttatagg ccgcaacaat cggcgcatag ccttgctcgg
    14101 ccagcacaat ctcgatcaga tcctgcatat cttcgatgtg cggaatgcgg cgaccatgtt
    14161 catcgggcga cacatgatat tcgctgcgcg gatcggacaa ctgttgaatc accagatcag
    14221 ccatttgctc gggcatggtt tcgcccactt ccacgtggtc gcggcggcgc acctcatcag
    14281 cagcgcggaa gattgccaac acgatcttgt cccggcggaa cggcaccacc atgccatcgc
    14341 gcttgcggaa cccgtcgaat acgatcgctt cagtcttgtt catgctcctg cgctcctttt
    14401 caccgctcca caaaatcata acggaacagg caacccgcag ttgccttgct ccgtttgatt
    14461 ttctgtgtag cccaagggcc atgcgtcaac aacccataaa gcccccatcc agccaaataa
    14521 tcattatagc acaacaactt gtggttgtta gttgtcaagg caactatatt ctgttatttt
    14581 gtggccgtaa tcgtttagtt atcaacaggt tacgggccgc aagtccgggg cttttgcccc
    14641 ttgggcaaat ccactccaaa accggccgtt ttctgcccca aaaacaagct cgggcaaggc
    14701 tgcaaaaacc cggaccgccc accggaatta tccccacgat cagctgtcga aaaccttcta
    14761 gccgttagcg gcgagatggt ggtgttccgc aatcattctc gcccttcatg agccaccgga
    14821 ttggattgga ggggtggagt tttcgggcta tccgcgtctg gccggatcga accagctcac
    14881 ttttctttgt gcggggacgt tcagcttccc cacactgcat tgttttagct gtttcgttag
    14941 ctgggcgggg tgaagcggat tttgccggcg cgagcgtggc cgtcgaggcc ttccacctgc
    15001 cccatgattt cgatgagcgg cagggtctcc aagagatcag cgcgggtgaa agcaatgacg
    15061 ctggagcgct tgcggaaaga atccacggag agccccgaga aaaacgcagc ggtgccaccg
    15121 gtgggcagca cgtggcttgg gccggccaca aaatcgccgg cactttccgg tgtccaaggg
    15181 ccaacgaaaa tcgcgccggc atttttgact ttctgcagcc agcggcgcgg ctccttggtc
    15241 atgatttcca gatgctccgg cgcgaaccgg ttgatgaggt ccatgccggt atccagcgta
    15301 tcggctacca cgaacattgc gccggcggca gcagctactt cgatggctgc gcgacgcgag
    15361 agcgactcaa tctgtcgttg catttccgcc agcaccttgg aggccatgct catgtagggc
    15421 gtcaccagca gggccttttc gtggccggtg ccgtgttcca attgcgaaat cagatcggcc
    15481 gcgacgtgcg ccggcacggc ggtttcgtcg gccagcacgg cgatttcgct ggggccggcc
    15541 accatgtcga ggtccacatc gccatacacc agctttttgg cggcggtgac atacgggccg
    15601 ccggggccaa caattttctg taccttgcgg atggtgttgg tgccgtaggc catcgcaccg
    15661 atggcctgga tgccgccaat acggtaaatc tcggtggcgc cggccacttc gagcgcgtgc
    15721 agcagataag ggttgacctt gccttgcttg tcgcacgggg tgcaggccac gatctccggc
    15781 acgccggcca ccttggccag cgagaggctc atcagcgcgg tggagaccag cggcgcggcg
    15841 ccgccgggga tataggcgcc gacgcgcgcc agcggcgtga accgctcgcc gagcacgccg
    15901 cccttgggcg aggccatgct ccaatccttg cgcatgccgg ccttgccaaa ccgcacgatg
    15961 cgcgtgaaaa tttccgccgc gctgcgcttg aacacaaggt ccacctcggc agaggcggca
    16021 aggacttcct cgcgcggcac cgcaaacttg gccactggca actgcaagcc gttgaattcc
    16081 ttggtgtact tctgcaccgc ccggtcgccg gtggcgcgga tgtccgccag cacgcggcgc
    16141 gccgtttcct cggccttggg atcaaacgca ggccgcaaca aaaacttctc cagctccggc
    16201 agctctttgt ggggtgaaaa ttttacaatg gggatggtca ggctcatggt caacctcgct
    16261 tcaaaaactt cgggtgcttt cgttcatcgt gccacatttt tcgcattcgg ccatcggaat
    16321 gttccggtgg tccagataga catgcccgca ccctgtgcaa cggaacacga agggcgccac
    16381 gcggtggcgc gggacatgcc ggtacctgcg cacggaatag tatagcgtga tcaagaagat
    16441 cagcgccaga tggctgacga aaataacggc gagccaatcg ggcagtggga gcggaatcat
    16501 ggctccgctc ccgtggcggg gccgggccgc acccacgcca ccgtgcccat tcgcggggca
    16561 ttgggttcca gcagccgcca cgcgctgacc gaacgcagca gccgctgatc ccaggacggc
    16621 acgcggcgtg ccgcgttcat cgaactgcaa ttccacccgc gcgcgccatg cggcgttggt
    16681 ccagtcgtca aagttcaggt caatgcccgc aaacaaccgc gactcccatc ccggtgaaaa
    16741 aaggaggcgc gcggactcgc gcgtggcgcg gcgcttggtc aacactccgc cggcggcggc
    16801 gccatcatcc gcgcgcggca actgccagcc ccattcgtcg gcgctggatt ccacaaccgg
    16861 ctccggcaac agataggcat aggccgggcg ctccatgtgc accgggggca gcagctccac
    16921 cttttcccgg cgcaaggcat gcgtgaaacc ggcgggcgtg gacaacgcaa acgctgccgg
    16981 ggtgtagagc gtacggatat cccatgcggc gggcggcgtg gcgcgccacc acgtcatgca
    17041 cgttggcgcg gggcgttcgg gtcccgccgg cgtcggcgcc aagcgaaaca gcagcgccat
    17101 cccgcccagc caaaacgcca caaaaacgcc ggcccagcgc cagccggtgc ggcaaaacac
    17161 ctgccagcgg cgggcggcgg cggaatggga gcgcagcttc ggcatgcccc atgccctatg
    17221 gcgccaccgg cggcggacgc gtggccagaa ccacattctg gatgccggct tcggcagcca
    17281 gggcatacac ctgcatcaag gtgccatacg gggtgccctc gtccgcctca accagcagcg
    17341 ccgaatcggc cggctcatcg ctgctcttgg ccaacgccgc gcccaagtcg gccaactcca
    17401 tcttctcgtc gttgtaaaag atcaggccgc cttgcgccag cgtcagcacc cgcgcgccat
    17461 agggcgcgcc gccggtgaat ggcgcggtgg gcagctccac ccgcaccccc ggttgcaaaa
    17521 tgaagcgcga cgacaaaaac atgaacatca tcaccagcgc catcacgttg agcagcgcag
    17581 tgacttcgat gggccccacg gcgcgccgga agcgcccttt aaagcgccga cgcacgctgc
    17641 gaaattgcag ttggatgggc tggtccggga ttctcatgcc ggcggggcct cccggttgcg
    17701 cttccactgg cgcataaacg tgagcaacgc cgaggccgag cgctccatat ccagcaaaat
    17761 ggactccaca cgcgacacca ggagattgta acccgcgtac gcgaggatgg cgataatcaa
    17821 gctcaacgcg gtggcggcca gcgcgtacca gagcccgccc gtcaggtcgg ccgactgcac
    17881 cagcggcgcg gcttccttgg ccgccaacag catttgcatc atgcccaaga ccgtgcccag
    17941 aagcccgatc agcggggtaa tgcgcgccag cgtggccagc cagcccaacc cgcgttccat
    18001 gcgcgggatt tcctccaaac cggcatcctc gatggcgcgg ccgattttct ccaagctgtc
    18061 gtcatggttg agcacggcgg ccagcgcgag gcgggccacc ggcccggggg catcctcgca
    18121 aagcgcggcc gcctccacga tgttatcgcg gttgaggacg ttgtaaatcc ccttgaggaa
    18181 gtcgtccgcc gggatttgcg cacggtgcag attgaacagg cgttcaaaga acaccagcag
    18241 ggcaaaaacg ccgcatgcca gcagcaaccc gatcaccaag ccaccgttca ccaatacatt
    18301 ccacatggtc tgtttctccg tccgttgctc ccaatgggaa gtggtgggcc agtctatgcc
    18361 gccccccccc gcccgctcaa tcacaaaatc ggccgcgcga gcacgatgcc gcaccattcg
    18421 ccatcgccat cgcgccggat ttcccgggcg cccaaccgca tgaaagcatc cgccacggaa
    18481 tcgccctcga actcgcgaat cccggtcagg atcagccgct ttttggtggc ctgccagagg
    18541 tcggccgccg ctgttagcaa aacactcgtc aaaatattgg cgcacaccaa gtccgccggc
    18601 tgccggcacc agcccgtttg caggatatcg gcctgataaa accggatgcg gcccttgggc
    18661 aagcggttca gaacggcatt ttcccggctt tgggtcacgc agaccggatc aaaatcaaaa
    18721 cccgccacct tggggacgcc caatttggcg gcggcaatgg ccaaaatacc gctgccggtc
    18781 ccggcatcca gcatggagcg cggcttgatc tcgacgcagg cccgctcgac ctcctccaaa
    18841 cagaagcggg tggtgaaatg gtcgccggtg ccgaagctca accccggctc tatccttata
    18901 ttgagtcgct tctggtcggg aaccttctcc caaatcggta cggttcgcag ccgttggcca
    18961 acatcctgaa tcttgaaatg atgccgccaa aatgtggtcc aatccttctc atggcagggc
    19021 atggcttggg cggcggattt ggcaaatcgg gccgggattt tccgccgatg ggtccgggct
    19081 ttggcggggg tagcaaaaaa tattttcacc acggtttggg ccccgccggg cttttcaaag
    19141 gccacgggtt cggttcccca tacctctcgg acccagtcgc aaaataattc tgcatctaca
    19201 ttgggcagaa caagttccaa ctgtccgatg gttttcattc gccagccagt atgccttgtg
    19261 gcccggcccc tgacaagtcc ggatcaagga tgggaacaat gttaggcggg cgggaaaatt
    19321 gacaatttga cttgcgcgga gaggctttac gtgtatcgtt ttacctgacg atatgaaacc
    19381 gatcaagtcc agccgtatat ggatcgtttg ggtggccatt ttggcgtttc ctctgggggg
    19441 caccgccttc cggcccgccc cacccgcccg cctgatgacc accgctccgg cggaatccgc
    19501 ccccgaaatg gccgccgcgg agaccgtgga ggctttagac ctcgccatgg cggcggcgga
    19561 tgaagaactg gccctgcgaa acgcccagtt actggtggat gccatcattc aaattgaatc
    19621 caatggcaat ccgcggatgg tgggccggca tggggagcgc ggcttgatgc aaatccgcgc
    19681 tggcacatgg cgggatatga ccacccggct ttttggccgc cccctcccct ttgaccgcgc
    19741 cttcgaaccc gagctgaacc ggcgcgtggg gacagcctat ctggcctatt tgcaggaaat
    19801 gctcctgcca cagcaggcag cgtggaaatc ggatgagcgc tcgctgctgc ttgcggccta
    19861 taatgccggt ccgggagcgc tgcggcaaac ccactacaac ttggcccaga tgccccgcca
    19921 aacccgtgac tacgtggccc gcgccagcgc cttgcacgat gtctatacct ccgacatcgc
    19981 ccaagcagat acccgcgaac tcgccgcggc cctgtagccc ccccgccccg cagggtcagt
    20041 caaaatacat cgcgacgtca aggcagtcga acgagccgtt gcaccagcgc acttgcagga
    20101 tggtcagccg gccatccagc acatccacat ccgccgccca agcgcgacgg gtgtcctcgg
    20161 catccagcac aacgcggtaa agccccggcg gcaagtcgaa gtagcggatg tgcccgctgc
    20221 gaatgctgtc cagatatttt ccttccacaa aaatctgcac gcgcccgccg gagatgttgt
    20281 ccaccacaat ggtcccctgc ccggccggcg ggtcgtggtc atagtcgtca tcatcgtgac
    20341 aaccattgcc caacgcgcac accgccgcca gcgccaacac gcccagccac ttgttcaaaa
    20401 tccggttttg ccatttcatg acgcgctccc tgtttagcag ccgaagccag catacaccct
    20461 gccgcgcggc ttgccaatcc cgcttcgcgc gcgaaacggg gcgccatcgc cattttctat
    20521 tattgcccgc cacgcggctg ttggcataga atcccgcccg aattatggaa gtggcggacc
    20581 aagggcccgc agcaaaacgg aaggatacat tcccatggaa cagattaaat taggtctgcc
    20641 aaaaggtagt ttgcaagaaa gcacgtttgc gttgatgaag aaagccggct ggacgttcaa
    20701 agtcggcgcg cggtcataca tgccgtcggt caatgatccg gccatcagcg cgcggctgat
    20761 ccggccgcag gaaattccgc ggtatgtcga gttggggctg ctggatgccg ggctgacggg
    20821 ctacgactgg atttacgaaa acggcgcgga tgtggtggaa gtggcggagc tgtgctactc
    20881 caaggccacc agcaacccgg tgcgttgggt ggtggcggtg ccaaacgatt cgcccatcca
    20941 aaccgtcaag gacctgcaag gcaagcgcat cgctaccgag gcggtcggcc tcacccgccg
    21001 ctatctctcc gaacacggcg tgacggcgga gatcgagttc tcctggggcg ccaccgaagt
    21061 gaaggcgcca gagctggtgg atgccattgt ggaactgact gaaacaggtt cctcgctgcg
    21121 tgccaaccaa ctgcggattg tggacaccgt gctcacgtcc accacgcggc tcattgccaa
    21181 caaggacacc tgggccgacg ctgaaaagcg cgccaagatt gagcaaatgg ccatgctgct
    21241 gaccggcgcg ctcaacgccg aaaacaaggt gctgctcaag atgaacgcca agaagggcga
    21301 cgttcccgcc gtgtgcggag tgctgccggc gctgcacgcg cccacggtca attcgctggg
    21361 cgatccaagc tgggtggcca tcgaaaccgt ggtggatgaa gccatcgtgc gcgaaatcat
    21421 cccgcgcctg aaaatggccg gcgcctccgg gattattgaa cttgctttga acaaagtcgt
    21481 gccgtaaata caaccggcaa tctaacaagg agtaaccatg ggttcggtca aaaagaaacg
    21541 ccgcctcaag atggccaagc acaagcgccg caaacggctg cgtgcggatc gccacaagaa
    21601 gaagtagtgc gggatacccc atgcgggcgg ccagtatagt tggcatttgc cgcagtcgct
    21661 tcgcactcgg gtgtctcgcc atcggcttgg cggtcggttg ccggtcgccg caaggccctc
    21721 ctcccgaacc ggggggaggg cttattgcgt accgtgattc cctctctccg gagcaggagc
    21781 gcaccgcgaa aatccatgcc cttttcgcgc aggcggtcca ccacacccac gccgacgagt
    21841 ttgccgaggc cgcggaattg taccgtcagg cccttgcgct ttcgcccacc aacgaagtac
    21901 tggtggtgcg cctggcggat acgatgcgcg tgctgcatca gaacgacgag gcgctcgccc
    21961 tcgtggaaca tttcgtggag caaaaccccg cggcggaaac tgccatgttc tggttggcgc
    22021 tgttttacaa gcaggccaac gaaacctcac gcggcatcga actgctgcgc cgcttgacgc
    22081 gcctgcatcc caccctgccg gcgggctggc tccaattggc ctcgctcacc gcgcgcgaaa
    22141 acgataccaa cgccgtggaa agtatcctgc gggaaggcat caccaaagcc aaaccgccca
    22201 cccccctccg ccggcaattg ttgcgcctcg aactaatccg ctacgtcggc gccaaggatg
    22261 atggcgaacg caaagcctcc cgcgagcgcg cggtggagtt gctcaaccag atcatcgcgg
    22321 ccgtgcccgg cgacttcgag gcattggccg ccctcggcga gctgctgacg gaagccaacg
    22381 actacaaggc cgccagcgaa atcttcgaaa ccctcacccg ccgtcagccc gccgtcaccg
    22441 agaattggga aaagctcgcc gggttgtatt tgctgctggg cgaacgggaa cgcgccatcg
    22501 ccacgctgga acaggtggcg cacaaattgg acctcccgcc tgacgcgcac tattcgctcg
    22561 gcaatttata ccgggatgcc aaggattata cgaatgccgc tgtccaatat aaacaggctg
    22621 cggaagcctc gccacacacg ccgcaaatgt gggtcatgtg ggcgctcatg tatagcgagt
    22681 ccgagccgga caagaccttc gaaatcctca accgggcgct ggagttgaat ccgaagagcg
    22741 cccaaatcat ggaaattact gcggcgcttt atcagcaaaa cgcaaaattt gccgaagccg
    22801 ccaaatggct gcaaaagctc catgagcttg ttgaaagcga acaggccacc gagcccccca
    22861 gccagtattt ttactttaat tttgcgttgg tctgcaccca tctgcgtcgc accgaggaag
    22921 cggcccaatg gctgcaacag gcggtcgagc aggaccccgc ttttctcccg gcctatgtgc
    22981 aagaaagttt tggcgccact gccagctatc ggcagcgcgc gctccaagtg ttgcgtgcgc
    23041 tggccaaacg gccgaccacc ttttccgccg aggcgcactt gcacctgggc aatatgtatg
    23101 cattccttga gcgcaacaaa gcggcagtgg ccgaatatga caaagtcctc gccattgttg
    23161 ccgccgatcc gctgcaagaa ggaatcctca acgcgcagtt ttatttttgg tacggcgtgg
    23221 tgctggacga gaccggccag accgagcgcg ccatccccat gtttgagcgt tgcctggaaa
    23281 aagattcctc attcgccaac gcgcacaatt acctcgctta tacgtgggcc gtgcgcaatg
    23341 agcgcttgga tgaagccttg cgccatatcc aaatcgcgct ggccatggaa ccggagaatg
    23401 ccgccttttt ggatacactg ggctgggtct atttcaagca aggccaatat gccgacgcgc
    23461 tcaaactctt ggaaaaagcc aacgagttgc gcccccacga cccggaaatc gaggaccacc
    23521 tcacccaaac acgggaaaag ttggcggccc ccgccggcaa ataactttcc acctcgtgcg
    23581 cgcgccccgc aaagcgttta ttttggccgc cggcctgggt acgcgtttac gcccgctgac
    23641 ccttacaacg cccaagccca tgctgccgct ttgggatcgc cccatgctcg cgcgaacgct
    23701 cgcgatgctc gccgattggg gcgtccagca ggtgctcatc aatttgcatc atgcgcccgg
    23761 ccccatcctg aactatctcg ccagccaacc gttcccgcaa ctgcgcatta ctgcagtgtt
    23821 cgagcccacc atcttgggca ccggcggcgc gctgcgcaac gcgcgctggt ttttggatga
    23881 accgtgctgg ctgctcaacg cggatattgt ggcccacgtc tccccccggc cgctgctccg
    23941 cgtttggcac aaggaacgcc cgctggccgc gtgctggctc acggatgccg ccggtcccaa
    24001 aacggtgcgc tgcgcggatg gctggattca ggattttcat cgcccgcaag atggtcaggc
    24061 cacgttttgc ggcctgcaac tgctggatca acgcatcttg cccttcattg cgccggaagg
    24121 tttcgatacc atcattgccg cctaccaacg cgcgcagcaa caaggccacc gtattgcggg
    24181 ggtttgcgtg cccggttcct tctgggccga tatcggcacc cccgcgcaat acgtccaagc
    24241 ccaccgcgat accgccggct tgtttggccg cgccggtgag cccttcaacc tgcccgcagc
    24301 cgcaacgttt tcctcgccgg agcaaaagtg cctgcgcatc actgtggacc acccggtgac
    24361 ggtcttgccg ccgcgcggtt ccaaccgcga gtttttcaag gttccggatt tgccgggaat
    24421 tagcggcccc gcgttggtga ttcgttggtc gcctgagcgc cccgaaaaca agctgtatgc
    24481 cggccacacc gtgtttttgc agaaactggg cattcccgtg ccgcgcctgt tgctgaatgc
    24541 gccgcgcgca cgcctgctgg cgatggaatt catcggcccc gacacgcttg aatcccgttt
    24601 accccgcctg acgcccacgc aagcgctccg tttgtatcgc caagtgctga ccatcacctt
    24661 gcgcttgcac acgcgcgggc tggccgtggc gcggcgcatc cggcgcccgc tcatgcccgg
    24721 ctttactccc cagctctacg catgggaaca cgattatttc attcaacacg tcatgcgcga
    24781 gctgctgcgc gcatcggctt cacgctgcgc gaaggttgcc gatgaactgc ggaccgtcgc
    24841 caacttgttg gcggcctcgc cgcaagcgtt gatccatcgc gattggcaat ccagcaatat
    24901 cctcctgcgc gggaatggcc cagtcatgat tgactatcaa ggaatgcgcc gcgggcccat
    24961 cgcctatgac ctcgccagcc tgctttgcga cccctacgcc aatttgcccg ccgcnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgacgc tgcgcctcgc gcaagcggcg
    25141 gcgcagcgtg gtgttgcgtc gaccggcttc cgcgttacgc accgccaagc ccacggcttt
    25201 ggtggtgccc accagcacca ccaactgctt gccgcgcgtg atgccggtat agagcaaatt
    25261 gcgttgcagc atcacgtagt gctgggtatg caagggcagc accacgcacg gatactcgct
    25321 gccctggctc ttgtgcaccg tcacggcgta cgccggggtc agttcatcca actcctgaaa
    25381 atcgtaggcc acctgccggt cgtcataccg taccagcacc tcttgctcgg cggcatccac
    25441 tttcacgatg gtgccaacat cgccgttgaa tacgtccttg tcgtaattgt tttccgtctg
    25501 catgacccga tcgcgctcgc ggtatttcca cccgtagcgc tccacttccc ggccctgcgg
    25561 attgagcgcg gcctgcatgg ccgcgttcaa attgcgcgcc ccaagctcgc ctttttgcat
    25621 cggcgtcaaa atctgcacgt cgcgcaacgg ttgcagccga aaccgccgtg gaatggcctc
    25681 gcaaaccaat ttgcggatca gctccacccc gtgcgccggc gtctcagcgc tgacaaaata
    25741 aaagtcgcta tgcccggcca acgcctcatc cttgcccggc agcaccggca tctggccgga
    25801 attgacgcgg tgcgcgttgc gcacaatttg gctgccatcg ctttgtcgga aaatttccgt
    25861 caaccggaac acggggatgg tgttggactc gatgatatcg cgtagcacgc agccggggcc
    25921 gacggatggc agttggtcca catcgcccac cagaatcagc aggccgtggc gcggcacggc
    25981 gcggaacaaa tgcaccgcca gttggatgtc aatcatgctc gtttcatcca cgatcaacac
    26041 atcggtttcc aagggcgact tggcgttata gcgaaagcgc ccgctgccgg gttcataggc
    26101 cagcatccgg tggatggtct tggcttccat cccggaaagt tccgtcatcc gcttggcggc
    26161 ccgccccgtc ggcgcgcaga gcatcagccg tagtt
//
LOCUS       DAOPNP010000019        25439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2461_length_25439_cov_9.208202, whole genome shotgun sequence.
ACCESSION   DAOPNP010000019 DAOPNP010000000
VERSION     DAOPNP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25439
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_25439_cov_9.208202"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..566)
                     /gene="crcB"
                     /locus_tag="PKI64_03020"
     CDS             complement(195..566)
                     /gene="crcB"
                     /locus_tag="PKI64_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOE00014.1"
                     /translation="MSAVLWVGLGGALGSIGRYLLSGWVTQQVTGRFPAGTLTVNVLG
                     CLGIGILAGLAVKEEYFSHAARLFLFTGVAGGFTTFSAFGLETFYLLQRAQWGLAGGY
                     VAASVAGCLLALAAGFAIVPR"
     gene            1178..3898
                     /locus_tag="PKI64_03025"
     CDS             1178..3898
                     /locus_tag="PKI64_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE00015.1"
                     /translation="MSIQLEDLEYQREAIDAVVKVLDGQMRNTFDNSNLLGIHTNTST
                     LTSEQVMANKKAVLAKTGVSEADAALTSEQEICIEMETGTGKTLVYFRTMYELCKEYG
                     LTKFIVLVPSIAIKEGTLAAFEDFREQLNNRYGLTPACFEYDSAKLSRLRAFLEDTHP
                     QIMLMTIQSIIGEDCIINRPGRDDSYGGETYLAALSKCRPIIVMDEPQEGMDTDAAKK
                     RIAQLNPLAILRYSATHKTVKNRLYRLTPFDAYQLGMVKKIEVLSVAEKNDEATHKIE
                     LADIQTHATRPPKAKLNLWRAQSGGFKWKVTNWLKVGDDLESASGNLSYRGYVIERIF
                     KSLHDGQWRLKFTNGVELLARERAADFSGLFRQQLHWLIRRHFQKKLGLDERNRPVGP
                     GLPARGIKPLSLIFIDRVDNYIKDDGIIKTLFREEYRALHSEYLKETPTDEQIAAVQG
                     YYFAKTGGGEYTDSENAMLKNREIFDLILRDKESLLRLDNPVEFIFSHSALGVGWDNP
                     NIFNIATLNQSYSEIKKRQEIGRGLRICRNQDGARVYDAEGTPEGREINLLTVIPNET
                     YETFATQYQAQILEDYGSTSASARLRQNDRGRPVKRTVRKTKLADDPAFKAFWDRLAR
                     KTDYTVQFREDEVVRRAIEALDRIVISAQEIETSLTRIQEIQAEGLQSTIIGTETNAS
                     RATFNPLDLVEEISENTALSYPAVFKIVQRFQNRAAVVKNPPRFLAHALDAIRRIELD
                     EMLRSLSYHPTGDAISLSELLAEVETSLDLEPTPNRGIYDHAICHSQPEHDFAHDADR
                     DNEVVCFLKLPEIYEIPTPIGSYHPDFGLVLRRRALRDGTENDYYFVIETKSTNNLDD
                     TRALTESERIKIKCALKHFQALQIEAKLTYLPYKAPVKEYKGDFKETLPT"
     gene            3975..4799
                     /gene="dinD"
                     /locus_tag="PKI64_03030"
     CDS             3975..4799
                     /gene="dinD"
                     /locus_tag="PKI64_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HOE00016.1"
                     /translation="MTATPSPFELIRRTNPAGNEFWSSRDFAKVLGYTDYRNFEAVIE
                     KARTACFNSGQRPEDHFVDITEMVEIGSGAQRALQAVMMSRYACYLVIQNADPAKEIV
                     ATGQTYFAVQTRRQEMTDQQLEEERRLLLRAEMKQHNVQLADAAKQAGVVEPIDYAIF
                     QNHGYAGLYGGLVAKDIHARKGLKTRQQILDHMGSTELAANLFRATQTEEKLRRDRII
                     GKDKANRTHRDVGAKVRQTIKELGGTMPEDLPPAESILKLEAKQRKALKPGKKKNT"
     gene            4796..6691
                     /locus_tag="PKI64_03035"
     CDS             4796..6691
                     /locus_tag="PKI64_03035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE00017.1"
                     /translation="MSPSPKNPLAAVSSPDLNRERLATLKKLFPDLFSNEGRLNLDEL
                     KKVVDPALVTETERYDFRWYGKTRSKRNAFTPSRAALVHDSTRSVNPDKANGNLIIEG
                     ENLEVLKLLTSAYRERIKCIYIDPPYNTGNDFVYSDNFAEGQKPYWEQTGVTQNGVKV
                     DTNTSSDGRFHSNWLSMMQSRLLVARYLLRPDGAIFVSIDDNELSNLRRIMDEVFGEE
                     NFLGCVIWQHSIQPKGYSGKFSVHHNFILCYQRSDVFELANLERTEEHNQNYSNPDND
                     PNGPWRTGDVRNALYRPNLIYDIDTPSGKKISPPQNGWRWSRETVAQKIASGEIVFSH
                     DESRIIRKIYLKNVEGRTPETIWFGKTVGTTRDANEELKQLFDSVVPFDTPKPSKLIQ
                     HMLQLSTDTESDNVVLDFFGGSGTTAQAVMELNKADGGNRKFILVQLPELTDEKSAAH
                     KAGFKKISDITIERVKRVIARIQDEASDKLPGDPQREFADSLGFKVYTLAKSHFPRVE
                     FAPDPAKTEAENIALLEQYIREKENTFDIQFNKDTVLDEVLLKNGFMLDYTLTPCPEF
                     TKNEVFRAKDAHKEALVCLDRQIAPETVDHFKGDKQSFFICLELALDTTKKWNLKHHL
                     SDRLNAM"
     gene            6697..7539
                     /locus_tag="PKI64_03040"
     CDS             6697..7539
                     /locus_tag="PKI64_03040"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HOE00018.1"
                     /translation="MLTTIQISSWPDFLEAIQHTQNQYGTFKRGDFVQHPTILYRGQA
                     NAEWTLATTLERISQSQWSLERYARLAYRCAPQIEAMTGRSWDMPKWETFEKALSNIY
                     DNHWVRMPLYNFCVYLRHHGFPSPLLDWTTSPYIAAYFALESNVEADAAIYVFIERPQ
                     GGKLLDGASCAVHTQGPYISTHKRHFLQQCFYTIASKCDPEQKTVWIMPHESILKLKR
                     PDQDIFIKYVLPKKLRFEALEYLQAHNITHYSLFQSDEALMRTLAFQEIERHEKEAEK
                     RPVL"
     gene            8223..8402
                     /locus_tag="PKI64_03045"
     CDS             8223..8402
                     /locus_tag="PKI64_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00019.1"
                     /translation="MSKSMIVGLVILAVFILLLLKADGNVVVQLGSWGRKISAAYALL
                     AAAGVGVVVGALLRK"
     gene            8399..9568
                     /locus_tag="PKI64_03050"
     CDS             8399..9568
                     /locus_tag="PKI64_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOE00020.1"
                     /translation="MKPLEELLLRGVRISAPGTVDIGDDVVPEQIAPGVVIHPGCRIY
                     GAKTSIGPGCVLGREAPLTLEDCQLGSGVQLKGGFCHGAVFLDGAVLGSAAQVRPGCI
                     LEEGVELAHAVGLKQTIMLPFVVGGSLLNFCDVLMAGGRNRREHSEIGSSFIHFNFTP
                     YGDKATASLVGDVPRGVMLDQTPIFLGGQGGMTGPLRLEYGTVLAAGHVLRRDVLTGG
                     QLVVPPPIPATERPFRRNGRAVGRVLKNNAIFVGNLAALAAWYDVARRPLLDRTPYGV
                     ACLTGALRQLAGARQERLKRLDDVTAWLAADEAATEADKRFCAAWPTAREQLAALGDQ
                     PGPEREAFVAAWHAAPEGDYLARIQKLPAEAKAAGTAWLQGIVDAAAGIMAAATA"
     gene            9711..10298
                     /locus_tag="PKI64_03055"
     CDS             9711..10298
                     /locus_tag="PKI64_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOE00021.1"
                     /translation="MTRMKVLMAAVALALAASVGWAAELRVATVDLDKVFNAHPRTKA
                     AEAELKKQEDEVQAEMERIMAEGQALRDEVTKLRAAAKNQLLTEEARREKSIAADDKL
                     NELQDFELRARRTQETKIKQMREQVLKSRQAIVDELMAAVTEFATAGEYDLVLDRSGQ
                     TMNAIPLVLYADPALDVTAQLIVFLEAKSGEVSKK"
     gene            10313..11362
                     /gene="lpxD"
                     /locus_tag="PKI64_03060"
     CDS             10313..11362
                     /gene="lpxD"
                     /locus_tag="PKI64_03060"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOE00022.1"
                     /translation="MFTLTTGELAAKLGATLEGAADRPLTGVADLRGARAGHVSFAGN
                     PKYLGQVADSAASAVIVPQDAVIESPQPSLLRVADADAAFAAACALFAPPPVVLPRGV
                     HPQAWVSPEAKLGANVAVGAFAVVEAGAEIGDGTTLYPQTYVGHAARVGRDCLLYPFA
                     GVRERCVLGDRVILHSGAVVGSDGFGYTVDAEGVRTKIPQTGIVVLEDDVEIGANTAI
                     DRARFGETRVCRGTKVDNLVQIAHNCIIGEHSVLCGQMGMAGSTTLGKRVICAGQVGL
                     AGHLKIGDGAVIGAQAGVAGDLPGGQMYLGAPAVPRLEFGKSLAHVAGLPKLKEQLKS
                     LAARVAALEKSAPGP"
     gene            11433..14948
                     /locus_tag="PKI64_03065"
     CDS             11433..14948
                     /locus_tag="PKI64_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOE00023.1"
                     /translation="MVLTKATEKQVAQLATQAQEGFLDNLTGAFFRRIDWAAFWVVLV
                     ATLGVYVYTLAPTLTLEDSGELAVASDYLGVPHPPGYPIWTLVTWFFQWIFHWVKYNG
                     HPNPAWSVGLASAVAGAGACGLLALVISRSGADLLRSIPALTEKIGFRMENLLCLVAG
                     ISGGLLLAFSPVLWSQSVIVEVYSLNALFQMAILMLIYMWMCRPKQDNLLYVVAFLFG
                     LGLTNHQTLLFLGLALAVAVLLKDADLFRDFAIAAVLIGIVFIFAAKGYSLIASAAEK
                     AAPDFDANLWKWSRGPTTAPFWIYNVLFLAIPIVLAFVLPRGRTVCATILLAEIGVAF
                     YMYLPFASEQNPPMNWGYPRTWEGFMHAVTRGQYEAIVPTDIFSEKFIQQLGAYLSDL
                     RGQFSLPVAVLGFLPFCAWGLRLGGQRRSVFPVTLGLVLASSVMIVLETLTGWGWLER
                     LYRLMIAPILLVAGWGFLTLLALYLKKQVADMRGYTLSTTIMIWGFLAVVAIGVLFVD
                     VMSLRAVFHSFGVKEISEPGYLSGVGRLFYLGLVVGPPLAVAVAWALNRSPLELDFEL
                     GVSQQRWLLTSVVGFISVSVIFLIFQNPSLDIQNLFIGRVQFIQSHAFYALWLGYGSL
                     LSVAWIERLLGRRALMPGLLAVLLLPGILLWQNAYDEEQIRIVGGAEQNGHSYGWQFG
                     NWGLEGIKGIKEDFQYWFPKAADFEREWGALTNALGMAEPDYPPPMTPNAIFYGGTDP
                     GRFVPTYMIYSANVRPDVYLITQNALADNTFMNITRDLYGDTIYIPSLEDSNEAFRKY
                     VDDVNSGRIQAGADIKIENGKVSVQGVGGVMTINGILAELIFKENKAKHDFYVEESYV
                     IQWMYPYLTPNGIMLKLNRDPVMELDPKLVENDHKFWGWYTQWLLDQSKFKRDICARK
                     SFSKLRSAIAGIYDYRRMYTEAEYAFKQSIDLYPLSPEANFRLAQMYMNMMRYDDAEQ
                     LIRGFANADPLNESAKGFLRQIESAKRMLNRRREIEKRQQETGTLTLDEALELTSIYR
                     ASGMQNAYQNLAMRLVADPNLPLQHVLALANFFAQDQRVNALIYALEQYTQRDPNNLD
                     AWLDLAAGYAFVRRDADAIRATRQSIQLGGDQARDAILHDPRFQPLATNREFRALFTR
                     PGSGGMRPLAPLPGGALPNLPGLAR"
     gene            complement(14973..15695)
                     /locus_tag="PKI64_03070"
     CDS             complement(14973..15695)
                     /locus_tag="PKI64_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOE00024.1"
                     /translation="MCGRYVTPDKEAVLLRWPKIRCDVQTTWQPGTNVAPTRTVPILI
                     QAPDGGLAVQAARWGFIPPWWSKPKPPGLTFNARVEEAATKPMWCDGFRGQRCLLPAR
                     GWYEWNNAERILDPAGRDTGQPYFIYQPAEPIMVIAGLWSVRADTGQPSYTSAMLTRA
                     AVPDIAFIHPRMPVVLPMESIAEWLDPATPATRVNEILAMPGAGFAAHPVSPRVNQVR
                     NEGADLMQKVQTPPASLFGPDD"
     gene            complement(15770..16339)
                     /locus_tag="PKI64_03075"
     CDS             complement(15770..16339)
                     /locus_tag="PKI64_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00025.1"
                     /translation="MNHIKFVSWLLILHIGISAMGGILGIALGIWHSPLSSYVAGAGM
                     LGPVALTIALWIAKQKTQPRTPVFVSAVIICGIWVGLLFLVMFPAPPEGKFIFKPDID
                     TRFADGYSDAAFATVAIGMNVSDVLALLGEPISTYESANWSFPGDAKTLWWYASDGAC
                     AWGDFAWRAPIIGIRDGVVVSKWTQWCYD"
     gene            complement(16398..17186)
                     /locus_tag="PKI64_03080"
     CDS             complement(16398..17186)
                     /locus_tag="PKI64_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE00026.1"
                     /translation="MKEQETEALVKAANATDTVAIEDLVNKGVPIDSPNKTGWTALMA
                     VANKGHLELVQFLTERGANVNARNQNGWTPLMWATLKGYTAIVQFLADHGAELDTRGE
                     DGNPPIHLAASGGFVEIVDFLLARGIAVNTENKNGQLPLMAAARNGRLEVVQTLLAHG
                     AGTNDGGHENNPPLVLASIGGHLEIIQLLLEKDADVNAKGRTGRSPLMAAAQRGDLDI
                     VRFLLERGADINARNDYGKTACRLAAADHRDEVVAFLREQGGEM"
     gene            complement(17513..18349)
                     /locus_tag="PKI64_03085"
     CDS             complement(17513..18349)
                     /locus_tag="PKI64_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00027.1"
                     /translation="MTPDKRFIQLPKSFWALVRLIGQECGYAAGGQITVASKAEVQAA
                     LAKLKLDPKSLDTALPDGRPVWKTLVAYFTHRRDTLHGQVEPNLMDSKQAKIEFTKLK
                     RQLKPTCPLPMNKQKGMKKAPAYLTGMVNMLVEANAGEHQCDYDPRALATIATNGLPL
                     RTFARRMDGAFPSVINPIAVWEVKEYYYTTTFGSRVADGVYETLLDGMEIEELLLTEK
                     MNVLHYLIIDAHYTWWDCGKSYLCRIVDMLHMGYVDEVLVGKEVISRIPELVKTWVKK
                     MA"
     gene            complement(18339..18584)
                     /locus_tag="PKI64_03090"
     CDS             complement(18339..18584)
                     /locus_tag="PKI64_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE00028.1"
                     /translation="MKHNRYDKNEESLRRLLRQKRTEMGLTQEALAAILDKPQSFVSK
                     YESGERLLSFVETVTVCRALGIPPHALLKEYLPNHDT"
     gene            18641..19534
                     /locus_tag="PKI64_03095"
     CDS             18641..19534
                     /locus_tag="PKI64_03095"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOE00029.1"
                     /translation="MKVKEDTLDLFYGTEFQHVAPFRTQLLKWIGNKQRFAHNIASFF
                     PADIQTYFEPFLGSGAVLGALQPPRAIASDACGPLMEIWQTLNNDPDLLVRWYRARHK
                     EYEQHGKPDGYERIKARYNASPNGADLLFICRSCYGGVVRFRKRDGYISTPCGIHDPI
                     SPTAFAERVQIWRRRTAGARFLNVDFEESMAMAQSGDLVYCDPPYVCSQTILYRGQDF
                     SLGRLMNTIADCKTRGVRVALSIDGKKKSGRVECDLDIPDGLFEREVYVDCGRSMLRR
                     FQREGESLEDEVVHDRLLLTY"
     gene            complement(19696..22035)
                     /locus_tag="PKI64_03100"
     CDS             complement(19696..22035)
                     /locus_tag="PKI64_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE00030.1"
                     /translation="MSLEKTKNFKQVNHSVTKVDAKQLALGKPSYVADFVPRDALIVK
                     MLWSPHPHARIVSMDVSAAEKMPGVKAVLWHGNVPRIPHTTAGQGFPEPSPYDTFMFD
                     TKVRFVGDRVAAVAAETEEQAKAALAKIKVVYEKLKPVFSLDEAMAEGAPIIHDEPEA
                     HVPIPIPYEPKKNLVARVGCDFGDVEKGLKEADFTFDEVFETPYAQHCPIEPYVSLAQ
                     VDSAGRIVITTSTQVPFHCRRIVAQTLDIPVQKIRVIKPRIGGGFGSKQEVLLEDVVA
                     MFALRTGRPCFWQLTREENFRYGRTRHPIRFRLRTGVKKDGTITAISMDCWNNTGAYG
                     GHGLTVVSCCGSKVLPLYRCENVHFLGKVYYTNLPCGGAYRGFGATQAFFAMESVIDK
                     MAEAIGMDPLKFRQLNHIKTGEGSPVFEALGEGKEGVAMTVGSCALGECITQGAAAIG
                     WERRTDWRGKTGRYRRGLGMAALMQGSSIPHIDMGGASIKMNEDGSFNLLVGATDLGT
                     GSDTVLAQIAAEELCTTTDKMIVYSSDTDMTPFDVGAYASSTTYLSGEAARKAAADAK
                     RQILHTGAEMLNLPEDQVICKDAHVQSKDGKQQVSYGDICCYALYQKNQYQIIGTASH
                     ITQKSPPPFAAHFAEIEVDTWTGNIKVLNYVATVDCGTPINPALCEGQTEGGTLNGIS
                     YALTEQYLFNKNGRMTNASFADYHIFSMRDKPPIKSILVPSYEDTGPFGAKSVSEICI
                     NGPAPAIGNALYNATGVRLHEFPFTPERVLNALRAAGNP"
     gene            complement(22077..22577)
                     /locus_tag="PKI64_03105"
     CDS             complement(22077..22577)
                     /locus_tag="PKI64_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOE00031.1"
                     /translation="MIKHPETVTFTLNGELKTFITRADEKLLDLLRREGYKGTKYGCG
                     QGTCGACTVLLDGRAVYACLLYSMQAENREVRTIESVGTHDQPSEFQQELIKAGAVQC
                     GFCIPGMIMSAEALMKAEDSYPPDVVREYMDGNLCRCTGYEKIWDALQKVLDRKQAGK
                     GSAKSS"
     gene            complement(22577..23422)
                     /locus_tag="PKI64_03110"
     CDS             complement(22577..23422)
                     /locus_tag="PKI64_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOE00032.1"
                     /translation="MKLCDFIRPASLAAARDNLRELGPKGMILAGGTALHFMQDHTPV
                     TAVDITRLGLSFIKQEGNYYNIGATTPLADIQRYRAPRWAMHEICRRISTHQIRNIST
                     IGGNICRVFPWADLPVILLAMDARMVIYSGQEEEFEADEYFKTQPAKRFLDGNILTSV
                     KVPVLHAHHGFGSIKVTRTDAAFAHVTIAAFLELDGNQIKSARMAAGSAIPFPRRLPT
                     VEAALVGREATPAVFKEAASKAGEDVTWRGREGMTADYSRHLAEVTILDALERAASFA
                     RGRTE"
     gene            complement(23525..24979)
                     /locus_tag="PKI64_03115"
     CDS             complement(23525..24979)
                     /locus_tag="PKI64_03115"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOE00033.1"
                     /translation="MDLVYVITPAILIFVVLAAVWLDRWSVPVILVALGGGMFFGSDI
                     LQWVHFDNATLANQVANFALVFILFQGGFGIKRSDFQAVALPAGGLAVWGVLLTAGAT
                     FLVLWKLLAWPFSHACLIAAIISSTDAAATLSILHRFALPKKLSTALVLESAANDPMA
                     VLLTTVVVNALVVGHGQGVFLLLKFAWKFAAAPVFGWLMARAALWLFNVLRPQDRGHY
                     YVLFLGVVLLTYGVTETLRASGMLAVFIAGYVMGNDAFVHKRGVATFSSAFASIANIC
                     TFVLLGLLVFPRQWGDIWLKGLILFLVITFISRPIAVFLGTPKMGLGFKNRLFMTWAG
                     LRGSVPIILATYPAAAGLPESQEIFNLVFFAVLLSVLVQGSTLGVCARALGLLTARTR
                     QEPLYSLELITMAPSDMDLLVVDLPESIGTDGPRIQDLRLPPDTVITLISRGEEVISP
                     LGATQLKGGDQITVLAHAKNSAGVRAALLGGARG"
     gene            complement(25047..>25439)
                     /locus_tag="PKI64_03120"
     CDS             complement(25047..>25439)
                     /locus_tag="PKI64_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE00034.1"
                     /translation="GTDGAATNNRYDMFSEMRTAALLAKVNAMDPTVCAAADIYRTAT
                     ADGFNIFRLDAGEIAVGKAADCILVDLNHTGLVPGHDLISDLVYAATPECVDTTICNG
                     KVLMRRRQIPGEDEIRTAFRAAAASCAP"
BASE COUNT         5314 a         7380 c         7698 g         5047 t
ORIGIN      
        1 aagaaatcct ccgcatgcag gtgctgcgca cagcggacaa ggcccggcaa ggcctcttta
       61 tgattgcgcc acgtaccgcc gccgtcaggg ctgttggtca ttcctgtagc cggcctcggt
      121 gaggccgggg ctgttggctc ggcggttttt acctgacctc tgaatcctta cctctgacct
      181 ctctttgggg ccgttcagcg cgggactatt gcaaaccctg ccgccaacgc cagcaaacaa
      241 cccgccacgc tggcggccac atagcccccc gccaaccccc actgcgcgcg ttgcagcaaa
      301 tagaaagtct ccagcccgaa cgccgaaaac gtagtgaaac cacccgccac tcccgtgaac
      361 aaaaagagcc gcgctgcgtg cgagaaatat tcctctttca ccgccagccc cgcgagaatc
      421 ccaatcccca agcaacccag cacattcacc gtcagtgtcc ccgccggaaa acgccccgtc
      481 acctgctgcg ttacccagcc cgaaagcaaa tagcgcccga tggatcccaa cgctccgccc
      541 agaccgaccc acagcactgc actcatgaaa ccgcaacctc tctttcgttg cgccgcaccc
      601 taccgcccgc ccctgtaccc cggcaagatt ctttgtcggc cgttgttgca atctccggcg
      661 gcctgttggc ccgggttgtc ccatttcttt ctgcgtttag ccaggaggat ttcttgcgga
      721 ttttggcgac gccaaaacca accggcgtct ttcccgcgct ggcgccgccg cctcgcggaa
      781 cgctcggtgg cagaggtcag ggttcagggt tcagagttca gaggtttttc tgtagccgtg
      841 ctcgctgagc acggggctgt tgggtcgtcg gctgtttcgg ctgttggcta ttcctgtagc
      901 cggcctcggt gaggccgggg ctgttggctc ggcatttttt cccctgacat ctgaaccctg
      961 ccccctgaac ccttcgccgc cttgcggcgg cgcaacagtc ccgccaaaat ccgcaagaaa
     1021 tcctccacag gcacgtgctg cgcacagcgg acaggagatg ggatcaggaa aagttcgggc
     1081 ggagtgtaag gtgtggcaat cctatcttcg gagtgctttt actggttatt tccttgggct
     1141 cctgctacgg tctatcccat aacctgtcgt tcggaagatg agcattcaac ttgaggattt
     1201 ggaatatcag cgcgaagcca ttgacgccgt ggtgaaagtg ctcgatggcc aaatgcgcaa
     1261 cacctttgac aactccaatc tactcggtat ccacaccaac acatccacac tgacctccga
     1321 acaggtcatg gccaacaaaa aggccgtgct cgccaaaact ggggtctccg aggccgacgc
     1381 tgcgctcacg tccgagcagg aaatctgcat cgagatggaa acgggcacgg gcaaaacgct
     1441 cgtctatttc cgcacaatgt atgagctttg caaagaatat ggcctgacca agttcattgt
     1501 tttggtgccc tccatcgcca tcaaggaagg aacgctggcc gccttcgagg atttccgaga
     1561 acaactaaat aaccgctatg gtttgactcc cgcttgtttc gagtatgaca gcgccaaact
     1621 ctcgcggctg cgcgccttcc tcgaagacac ccacccgcaa atcatgctga tgaccattca
     1681 atccatcatc ggggaggatt gcatcatcaa tcgtcccggt cgtgatgatt cctatggcgg
     1741 cgagacatat ctcgcggcgc tgagcaaatg ccgaccgatt atcgtgatgg atgagccgca
     1801 agagggcatg gacacggacg cggcgaaaaa acgaatcgcc cagctcaatc cgctggccat
     1861 actgcgctat tcggccactc acaagaccgt caagaatcgg ctataccgcc tgaccccgtt
     1921 cgatgcctac cagcttggaa tggtcaaaaa aattgaggtt ttgtcggtgg ccgaaaaaaa
     1981 tgatgaggct acccacaaga tcgaattggc cgacatccaa acccacgcca cccgcccgcc
     2041 caaggccaag ctgaatcttt ggcgcgcgca aagcggcggc ttcaagtgga aagtcaccaa
     2101 ctggctcaaa gtcggcgacg acctggaaag cgcctccggc aacctgtcct atcgcggcta
     2161 cgtcattgag cgaatcttca agagcctgca cgatggccaa tggcgcctga agttcacgaa
     2221 tggcgtggag ctgctggcaa gggaacgcgc cgccgacttc tcgggcctgt tccgccaaca
     2281 actccattgg cttatccgcc gccactttca gaaaaaattg ggtctcgacg aacgcaacag
     2341 gccggtcggg cccggcctgc ccgcgcgcgg catcaagccg ctttccctga tctttattga
     2401 tcgggtggac aactacatca aggatgacgg catcatcaag accctgttcc gcgaggaata
     2461 ccgcgccctg cacagcgaat acctcaagga aacaccgacg gacgagcaaa ttgccgctgt
     2521 gcaaggctac tacttcgcca aaacaggcgg cggcgaatac accgacagcg aaaatgccat
     2581 gctcaaaaac cgcgagatat tcgacctgat cttgcgcgac aaagagagcc tgctccgcct
     2641 tgataatccc gtggaattca tcttctcgca ctccgccctt ggcgttgggt gggacaaccc
     2701 caacatcttt aacattgcca ccctcaacca gtcctacagc gaaatcaaaa agcgccagga
     2761 aatcgggcgc ggcctgcgca tctgccgcaa tcaggacggt gcccgcgttt atgatgccga
     2821 aggcaccccc gaaggccgcg aaatcaacct gctcaccgtc atccccaacg aaacctacga
     2881 aacctttgcc acccaatatc aggcgcagat tctcgaagac tacggcagca cttccgcctc
     2941 cgcccgcctg cggcaaaatg acagggggcg ccccgtcaag cgcaccgtcc gcaaaaccaa
     3001 gctcgccgat gatcccgcat tcaaggcgtt ttgggaccgc ctcgcccgca agaccgacta
     3061 cacggttcaa ttccgcgagg acgaagtcgt ccgtcgcgct attgaagccc tcgatcgcat
     3121 cgttatttcc gcccaggaaa tcgaaacctc attgacgcgt attcaggaaa tccaagccga
     3181 aggcctgcaa agcacaatca tcggcaccga aaccaacgcc agccgggcca ctttcaaccc
     3241 actcgatctg gtcgaagaaa tcagcgaaaa caccgccctt tcgtatcccg ccgttttcaa
     3301 gattgtccag cgcttccaaa accgtgccgc cgttgtcaag aacccgcccc gtttcctcgc
     3361 ccatgcgctc gacgccatcc ggcgcattga gctcgatgaa atgctccgca gcctgtccta
     3421 ccatcccacc ggtgacgcca tttccctgtc cgaactactg gcggaggtgg aaacttcgct
     3481 cgatctcgaa cccaccccca accgcggcat ctacgaccac gccatttgcc acagtcagcc
     3541 ggaacacgac tttgcccacg atgccgaccg ggataacgaa gttgtctgct tcctcaaact
     3601 ccccgaaatt tacgaaatcc cgacccccat tggcagttac caccccgact tcggcctcgt
     3661 cctgcgccgc cgcgccctcc gcgatggcac tgaaaacgac tactatttcg tcatcgaaac
     3721 caaatccacc aacaacctcg acgacacccg cgctcttacc gaatccgaac gcatcaaaat
     3781 caagtgcgcc ctcaaacact ttcaagccct tcaaatcgaa gccaagctga cttatctgcc
     3841 ctacaaagcc cccgtcaagg aatacaaagg ggacttcaag gagacactgc ccacatgagc
     3901 acggatcata tcgtcggata tttccccgcg cctttgcttt tcggcgccac aacgccaaag
     3961 aaagaccttc catcatgacc gcaaccccgt cgccttttga actcatccgc cgcaccaatc
     4021 ccgccggcaa cgaattctgg tccagccgcg acttcgccaa ggtcctcggc tacacggact
     4081 accgcaattt cgaggccgtg attgagaaag cccgcaccgc ctgtttcaac agcggccaac
     4141 ggcccgaaga ccatttcgtt gacatcaccg aaatggtcga aatcggcagc ggtgctcagc
     4201 gcgcactcca agccgtcatg atgtcccgct acgcctgcta tctggtcatt caaaacgccg
     4261 accccgccaa ggaaatcgta gcgacggggc aaacctattt tgccgtccag acccggcggc
     4321 aggaaatgac cgaccagcag ctcgaagaag agcgccgcct gctgctccgg gcggaaatga
     4381 agcagcacaa tgtccagttg gctgatgccg ccaagcaggc cggcgtcgtg gagcccattg
     4441 actacgccat cttccagaac cacggctatg ccggacttta cggtggactc gtcgccaagg
     4501 acatccacgc ccgcaaggga ctcaaaacgc gccagcagat actcgaccac atgggcagca
     4561 ccgaattggc cgccaatctt ttccgcgcca cccagaccga agaaaagctc cgccgtgacc
     4621 gcatcatcgg caaggacaag gccaaccgaa cccaccgcga tgtcggcgcc aaagttcgcc
     4681 aaaccatcaa ggaactcggc ggcaccatgc ccgaagacct cccgcccgcc gaaagcattc
     4741 tcaagctaga agccaaacag cgcaaggccc tcaaacccgg caaaaagaaa aacacatgag
     4801 cccatccccg aaaaatccat tggccgccgt ttcgtcgccg gacctcaacc gtgaacggct
     4861 cgccactctc aaaaagctgt tccccgacct gttctccaac gaaggccgcc tcaacctcga
     4921 cgaactgaaa aaagtcgttg atcccgccct cgtcaccgaa accgaacgct acgacttccg
     4981 ctggtacggc aaaacccgct ccaaacgcaa cgccttcacc cccagccgcg ccgctctcgt
     5041 ccatgattcc actcgctccg tcaaccccga caaggccaat ggcaacctca tcatcgaagg
     5101 cgaaaacctc gaggtcctca aactcctgac ttccgcctac cgcgaacgca tcaaatgcat
     5161 ctatattgac ccgccctaca ataccggaaa cgatttcgtc tattccgaca acttcgccga
     5221 gggccaaaag ccctattggg agcaaaccgg cgtcacccaa aacggcgtca aggtggatac
     5281 caatacgagc agcgacggcc gcttccattc caactggctc tccatgatgc aaagcaggct
     5341 gctggtcgcg cggtatttat tgcggcccga cggggctatc tttgtaagta ttgacgacaa
     5401 tgaattaagc aatttgcggc gaataatgga tgaagtcttt ggcgaagaaa atttccttgg
     5461 ctgcgtaata tggcagcaca gcatacagcc caaaggttac agcggaaaat tctctgttca
     5521 ccataatttc attttgtgtt accagcggtc cgatgtgttt gaattggcca atctcgaaag
     5581 aaccgaagag cacaaccaga attattcaaa tcccgacaac gatcctaatg ggccatggcg
     5641 caccggagat gtccgcaacg cgctttaccg tccaaatctc atttatgaca tcgatacgcc
     5701 ctccggtaaa aagataagtc cgcctcaaaa tggttggcga tggagcaggg aaaccgttgc
     5761 tcagaaaatt gcatcgggcg aaatagtctt ttcgcatgat gaatctcgaa tcattcgcaa
     5821 gatatatcta aaaaatgtag agggccgtac ccctgaaaca atatggtttg gcaaaacagt
     5881 tgggacgacg cgagacgcga acgaagaact caagcaacta tttgacagcg ttgtcccttt
     5941 tgacaccccc aagccatcaa aattgattca gcatatgctt cagttgtcca cagacacaga
     6001 aagcgacaac gtagtattgg actttttcgg cggcagcgga acaacggcgc aagcggtgat
     6061 ggagctaaac aaggccgatg gcggcaaccg gaaattcatt ctcgtccaac tccctgaatt
     6121 gaccgacgag aaaagcgctg cccataaagc cggcttcaaa aagatcagcg acatcacaat
     6181 tgagcgcgtc aaacgggtga ttgcccgcat tcaggatgaa gccagcgaca agttgcccgg
     6241 cgatccgcaa cgggaatttg ccgactcgct tgggtttaag gtttataccc tcgccaagtc
     6301 gcatttcccg cgcgtggaat tcgcgccgga tcctgccaag accgaggctg aaaacatcgc
     6361 cctgctcgaa cagtacatcc gcgagaagga aaatactttc gacatccagt tcaacaagga
     6421 cacggtgctc gacgaagtgc tgctaaaaaa cggcttcatg cttgattaca cgctgacgcc
     6481 ttgccccgag ttcacgaaaa atgaagtttt ccgcgccaag gacgcgcaca aagaggcgct
     6541 ggtctgtctg gaccgccaaa tcgcccccga gaccgtggac cacttcaaag gcgacaaaca
     6601 gagctttttc atctgcctcg aactcgccct cgacaccacc aaaaagtgga acctcaaaca
     6661 ccacctctcc gaccgcctga acgcgatgta aaacccatgc tgaccaccat ccagatttca
     6721 tcgtggcctg attttctgga ggccattcag cacacgcaaa accaatatgg cacttttaaa
     6781 cgaggagatt tcgttcagca tcccaccata ctctatcggg ggcaggcgaa tgcagaatgg
     6841 acactggcaa ctaccctgga aagaattagc caaagccaat ggtcgctgga aaggtatgcc
     6901 agactggcct acagatgtgc tccccaaatt gaagccatga caggccgcag ttgggatatg
     6961 ccgaaatggg agacatttga aaaagcacta tcaaatattt acgataacca ttgggttaga
     7021 atgcctctct ataatttttg tgtttaccta aggcaccatg gtttcccatc cccactgctg
     7081 gactggacga cctcgcctta cattgctgcc tattttgcct tggaatcaaa cgttgaagct
     7141 gatgcggcaa tatatgtgtt tattgaaagg ccacaggggg ggaaactact cgacggggcc
     7201 tcttgcgcgg tgcataccca aggcccttat atttcaaccc ataaacgcca tttccttcaa
     7261 cagtgtttct acacgatcgc gtcaaaatgt gatcctgagc agaagaccgt gtggatcatg
     7321 ccgcacgaaa gcattctgaa actcaaacgg cccgatcagg atatatttat aaaatatgtt
     7381 ctcccaaaaa agctcaggtt tgaggccttg gaatatctgc aagcccataa tattacgcat
     7441 tacagcctgt ttcagtcgga tgaggctttg atgcgcactt tggccttcca agagattgaa
     7501 aggcatgaga aagaggccga aaagagaccg gtcctataat cctgattttt gggagcaggc
     7561 caaatagccc gccggggatg ccccagccta caaaagcgtt gcgtgttgcg ccgtgcgcaa
     7621 gacgcttggc gcatcgtgtt tagtaatggg ggcgatgata tggcgtgcca aagctttttc
     7681 ctgctttcct gggtttcttg ttttcatgct gggttcgatg gttttccacg ctgtggaaaa
     7741 tagacgagtg ccgagcccaa gtccgcagga cgccgggtga acgcgagttc tatagcggcg
     7801 gcgcagccgt ccgagcaagc gagccatagc gagcgcagcg gcaaatcggc ggcaaaagtt
     7861 ccacaccgtg gaaattattt ttccacactg tggaaaaatt cacgcgtcac ggccctgttt
     7921 tttccacgca atggaaaata ggcgagtgcc gagcccgagc ccgaagggcg atgggcgaac
     7981 gcgagttcta tagcggccgg cgtagccggg cgagccagcg agccatagcg agcgcagcgg
     8041 caacgttttg gcgatttttc cacacaatgg aacaactttg ggccattatt tccacaccgt
     8101 ggaaaacccc gccacgccat ggcggggcgc gtgggcaaag tgtggggcgg ggcgcaaaaa
     8161 atgctgaaaa gttttgcggg gtgtggcaga ctgcggcggt caatcgaaga ggtgcgtggc
     8221 agatgagcaa gagcatgatc gtggggctgg tgattctggc ggtgtttatc ctgttgctgc
     8281 tcaaggcgga tggcaacgtg gtggtgcagt tgggatcgtg gggccggaag atttccgcgg
     8341 cgtacgcgct gttggcggcg gcgggggtgg gcgtggtggt gggggcgctg ctgcgcaaat
     8401 gaagccgctg gaagaattgc tgctgcgcgg cgtgcggata tcggcgccgg gaacggtgga
     8461 tattggcgat gacgtggtgc cggagcagat cgcgccgggg gtggtgattc atccggggtg
     8521 ccggatttat ggcgccaaga cgagcatcgg ccccggttgc gtgctgggac gcgaggcgcc
     8581 gctgacgctg gaggattgtc agttgggttc gggggtgcag ttgaagggcg gcttttgcca
     8641 cggggcggta tttctggatg gcgcggtgct ggggagcgcg gcgcaggtgc ggccggggtg
     8701 catcctcgaa gagggcgtgg agctggcgca tgcggtgggc ttgaagcaga cgatcatgct
     8761 gccgtttgtg gtggggggca gcttgctcaa tttttgcgat gtgctgatgg cgggcgggcg
     8821 caatcggcgg gagcatagcg agattgggtc gtcgttcatc catttcaatt tcacgcccta
     8881 tggcgacaag gcaacggcgt cgctggtggg ggatgtgccg cgcggggtga tgctggacca
     8941 aacgccaatt tttctcggcg ggcagggggg gatgacgggg ccgttgcgat tggagtatgg
     9001 cacggtgctg gcggcgggcc atgtgttgcg gcgcgatgtg ctgacgggtg ggcaactggt
     9061 ggtgccgccg cccattccgg caaccgagcg tccgttccgg cgcaatggtc gtgcggtggg
     9121 gcgcgtgtta aagaacaatg cgatttttgt ggggaatttg gcggcgttgg cggcgtggta
     9181 tgacgtggcg cggcggccgc tgctggatcg cacgccctac ggcgtggcgt gcctgaccgg
     9241 ggcgctgcgg caattggcgg gggctcggca ggagcgcctg aaacggttgg atgatgttac
     9301 ggcttggctg gcggcggatg aagcggccac cgaggctgac aaacgatttt gtgcggcgtg
     9361 gccgacggcg cgcgagcaac tggcggcgct gggcgatcag cccggccccg agcgcgaggc
     9421 gttcgtggcg gcgtggcacg ccgcgccgga aggcgattat ctggcgcgaa tccaaaagct
     9481 gccggccgaa gccaaggcgg cggggacggc gtggttgcag ggcatcgtgg atgcggcggc
     9541 ggggatcatg gcggcggcca ccgcctgaga ccagccaacg gcaaccaatc gcggcgggga
     9601 caacgcgtgg attgcgcggc gccttgccgc gtttttcggc ttttcaagcg cgggggggtg
     9661 ggggagaatg ccgcgcgagt cattttccag acaaagaaag ggaagaccac atgacacgga
     9721 tgaaggtttt gatggcggcg gtggcgctgg ctttggcggc gtcggtgggt tgggcggcgg
     9781 agctgcgggt ggcgacggtg gacctcgaca aggtcttcaa tgcgcacccc cggaccaagg
     9841 cggccgaggc cgaattgaag aagcaggaag atgaagtgca ggccgagatg gaacgcatca
     9901 tggccgaagg ccaggcgctg cgcgacgaag tcaccaagct gcgcgcggcg gccaaaaacc
     9961 agttgctgac cgaggaagcg cgccgcgaaa agagcatcgc ggccgacgac aagctcaacg
    10021 agctgcaaga ttttgagctg cgcgcgcggc ggacgcagga aaccaagatc aagcaaatgc
    10081 gcgagcaggt gttgaagtcg cgccaggcca tcgtggatga gctgatggcg gcggtgacgg
    10141 aattcgctac ggcgggcgaa tatgacctgg tgctggaccg ttccggccag accatgaatg
    10201 ccatcccgct ggtgctgtat gccgacccgg cgctggatgt cactgcccaa ctgatcgtct
    10261 ttctggaggc caagtcgggc gaggtttcca agaagtagcc ggaggtggcg gggtgttcac
    10321 gttgacgact ggtgagttgg ccgccaagct gggcgccacc ctcgaagggg cggcggaccg
    10381 gccgttgacg ggggtggcgg atttgcgcgg ggcgagggcg ggccatgttt cgtttgcggg
    10441 gaatcccaaa tatctggggc aggtggcgga cagcgcggca tcggcggtga ttgtgccgca
    10501 ggacgccgtg attgagtcgc cgcagccgtc gttgctgcgc gtggcggatg ccgatgcggc
    10561 gttcgcggcg gcatgcgcgc tgtttgcccc gccgccggtg gtgctgccgc gcggggtgca
    10621 cccgcaggcg tgggtttcac cggaggccaa gctgggcgcc aatgtggcgg tgggggcatt
    10681 cgcggtggtg gaggctgggg cggaaattgg cgacggcacc acgctgtatc cgcagaccta
    10741 tgtgggccat gcggcgcgcg tcgggcgcga ttgcctgctc tatccgtttg ccggcgtgcg
    10801 cgagcgctgt gtgctgggcg accgcgtgat tttgcatagc ggcgcggtgg tcggcagcga
    10861 tggctttggc tatactgtgg atgccgaggg cgtgcgcacc aaaattccgc agacgggaat
    10921 cgtggtgctg gaagacgatg tggaaatcgg cgcgaatacg gcgattgacc gcgcgcgctt
    10981 cggcgagacg cgggtgtgcc gcgggaccaa ggtggataac ctggtgcaga tcgcccataa
    11041 ctgcatcatt ggcgagcact ccgtgttgtg cggccagatg ggcatggcgg gctccaccac
    11101 gctgggcaag cgcgtgattt gcgccgggca ggtgggcttg gccgggcatc tcaagattgg
    11161 cgatggcgca gtcatcggcg cgcaggccgg cgtggcgggc gatctgcccg gcggccagat
    11221 gtaccttggc gcgcccgcgg tgccgcgatt ggaatttggc aagtcgctcg cccatgtggc
    11281 ggggctgccc aaactgaagg aacaactcaa gagcctcgcg gcgcgcgtcg cggctctgga
    11341 aaaatccgcg cccggcccgt gacggattga ggcgtggagc agggtagatg gacaggcggc
    11401 ccgcccgggc cccgcaacta cggatggatg acatggtatt gacgaaagcc acggaaaaac
    11461 aagttgccca actggccacg caggcgcagg aggggtttct cgacaatctg acgggcgcat
    11521 ttttccggcg cattgactgg gcggcgttct gggtggtgct ggtggccacg ctgggcgtat
    11581 atgtctatac gctggcgccc acgctgacgc tggaagactc cggcgagttg gccgtggcgt
    11641 cagattatct gggcgtaccg cacccgccgg gctatccgat ctggacgctg gtgacgtggt
    11701 tcttccagtg gattttccat tgggtgaagt ataacggcca tccgaatccg gcgtggtcgg
    11761 tggggctggc ctcggccgtg gcgggggcgg gggcgtgcgg cttgctggcg ctggtgatta
    11821 gtcgttccgg tgcggacttg ttgcgcagca ttccggcgct gacggagaag atcggttttc
    11881 gcatggaaaa cctgctgtgc ctcgtggcgg ggatttccgg cggcctgctg ttggcgttca
    11941 gcccggtgct gtggtcgcag agcgtgatcg tggaggtgta tagcctcaac gccttgttcc
    12001 aaatggccat cctgatgttg atctacatgt ggatgtgccg ccccaagcag gacaacctgc
    12061 tctacgtggt ggcgttcctg ttcgggctgg gcctgaccaa ccaccagacg ctgctgttcc
    12121 tcggcctcgc gctggccgtg gccgtcctct tgaaggatgc cgacctgttc cgtgatttcg
    12181 cgattgccgc cgtgctgatc ggcattgtat tcatctttgc cgccaagggc tacagcctga
    12241 ttgcctccgc cgccgaaaag gccgcgccgg atttcgacgc caacctgtgg aaatggtcgc
    12301 gcgggcccac caccgcgcca ttctggattt acaacgtgct gttcctggcc atcccgattg
    12361 tgctggcgtt tgtgctgccg cgcgggcgga cggtttgcgc cacgatcctg ttggcggaaa
    12421 tcggcgtggc gttctacatg tatctgccgt ttgcctcgga acaaaacccg ccgatgaatt
    12481 ggggctatcc gcggacatgg gagggtttca tgcacgccgt tacccgcggc cagtatgaag
    12541 ccattgtgcc cactgatatt ttcagcgaga aattcatcca gcagttgggc gcgtatttgt
    12601 ccgacttgcg cggccagttc tcgctgccag tggcggtgct gggcttcctg ccgttctgcg
    12661 cgtgggggtt gcggctcggc ggccagcggc gctcggtgtt cccggtcacg ctggggctgg
    12721 tgttggcgtc gtccgtgatg attgtgctgg aaacgctgac gggctggggc tggctggagc
    12781 ggttgtaccg gctgatgatc gcgcccatcc tgctggtggc cggctgggga ttcctgacgc
    12841 tgttggcgtt gtacctcaag aagcaagtgg ccgacatgcg cggttacacg ttgagcacga
    12901 ccatcatgat ctggggcttc ctcgcggtgg tggccatcgg tgtgctgttt gtggacgtga
    12961 tgagcctgcg cgcggtgttc cactcgtttg gcgtgaagga gatttccgaa cccggttatc
    13021 tctccggcgt gggtcggttg ttctatttgg gcctcgtggt tggtccgccg ctggcggtgg
    13081 ctgtggcgtg ggcgctcaac cgctcgccgc tggagttgga ctttgaactg ggcgtatcgc
    13141 agcagcgttg gctgctgacc tccgtggtgg gattcatttc cgtaagcgtg atcttcctga
    13201 ttttccaaaa tccgtcgttg gacattcaga acctgtttat cggccgggtg caattcatcc
    13261 aaagccacgc gttctacgcg ttgtggctgg gttatggcag cctgctttcg gtggcgtgga
    13321 tcgagcgact gctggggcgg cgcgcattga tgccgggact gctggccgtg ctgctgctgc
    13381 cgggcatcct gctgtggcaa aacgcgtatg acgaagagca aatccgcatt gtcggcggcg
    13441 cggagcaaaa cggccacagc tacggctggc aattcggcaa ctgggggctc gaaggcatca
    13501 agggcatcaa ggaagatttc caatactggt tccccaaggc ggccgatttt gaacgcgaat
    13561 ggggcgcgct gaccaacgcg ctgggcatgg ccgagccgga ttacccgccg ccgatgacgc
    13621 ccaacgccat cttctacggc ggcaccgatc cggggcgttt tgtgccgacc tatatgatct
    13681 attccgccaa cgttcggccg gatgtctatc tcatcaccca gaacgcgctg gccgacaaca
    13741 ccttcatgaa catcacccgc gacctctatg gcgacaccat ttacattccg tcgctggagg
    13801 acagcaacga ggcgttccgc aagtacgtgg acgatgtcaa ctccggccgc attcaggccg
    13861 gcgccgacat caagatcgaa aacggcaagg tcagcgtgca gggtgttggt ggcgttatga
    13921 ccatcaacgg cattctggcc gagttgatct tcaaggaaaa caaggccaag cacgatttct
    13981 acgtggaaga aagctacgtg atccagtgga tgtatcccta cctcacgccg aacggcatca
    14041 tgctcaagct gaaccgggat ccggtgatgg agttggaccc caagctggtc gaaaacgacc
    14101 acaagttctg gggctggtac acgcagtggc tgttggacca atccaagttc aagcgcgata
    14161 tttgcgcgcg caaatcgttc tccaagctgc gcagcgccat cgccggcatc tatgactacc
    14221 gccggatgta taccgaggcg gaatatgcct tcaagcagtc cattgatctc tatccgctct
    14281 cgcccgaagc caacttccgc ctcgcgcaga tgtacatgaa catgatgcgc tacgacgacg
    14341 ccgaacaatt gatccgcggg tttgccaacg cggatccgct caatgaaagc gccaaaggat
    14401 tcttacgcca gatcgaaagc gcgaagcgca tgctgaaccg ccggcgcgaa attgaaaaac
    14461 gtcagcagga aacgggcact ctgacactgg atgaggcgct ggagctgacc tcgatctatc
    14521 gcgcctccgg catgcagaat gcctaccaga accttgctat gcgtctcgtg gcagatccca
    14581 atctgccgct gcagcacgtg ttggcgctgg ccaacttctt tgcgcaagac cagcgggtga
    14641 atgcgttgat ttatgcgctg gaacaatata cccagcgcga ccccaacaac ctggatgcct
    14701 ggttggattt ggccgcgggc tacgcttttg tgcggcgcga tgctgacgcc atccgcgcca
    14761 cacgccagag catccagttg ggcggcgatc aggcgcgcga tgccattctg cacgacccgc
    14821 gcttccaacc gctggccacc aatcgcgaat tccgcgcgct cttcacgcgg ccgggcagcg
    14881 gcggaatgcg gccgctggca ccgctccccg gcggcgcgct gcccaacctg cccggtttgg
    14941 cgcgctgacg ccccgcctgc gcccgcggga catcaatcgt cggggccgaa caggctggcc
    15001 ggcggggttt ggactttctg catcaggtcc gcgccctcgt tacgcacttg gttcactcgt
    15061 ggcgaaaccg ggtgcgccgc gaatccggcg ccgggcatgg cgaggatttc gttcacgcgc
    15121 gtggcgggag tggcggggtc cagccattct gcgatgcttt ccatcggtag cacgaccggc
    15181 atccgcggat gaatgaacgc aatgtctggc acggcggcgc gggtcagcat ggcgctggtg
    15241 tacgacggtt gccccgtgtc cgcacgaacc gaccacagcc ccgcaatcac catgatcggt
    15301 tccgcgggct gataaatgaa gtagggttgg ccggtgtcgc gtccggcggg atcaagaatt
    15361 cgctctgcgt tgttccattc ataccagccg cgggcgggca gcaggcagcg ttgcccgcgg
    15421 aaaccatcgc accacattgg tttggtcgct gcctcttcca cccgcgcgtt gaacgtcagg
    15481 cccggcggtt tgggtttgct ccaccacggc gggataaatc cccaccgcgc cgcctgcacc
    15541 gccagaccgc catccggcgc ctgaatgagg atgggcaccg tccgggtggg cgccacgttg
    15601 gtgccgggtt gccaagtggt ttggacgtcg caccggattt tgggccagcg cagcagcacg
    15661 gcctctttgt ccggagtgac gtagcgtcca cacatgggct caacgatagc gatgaggctg
    15721 aggtggttta ccgccaatag cctgaagcgc ctttttcgac acctgcattt cagtcatagc
    15781 accactgcgt ccatttactg acaaccacgc cgtctcgaat gccaataatg ggtgcacgcc
    15841 acgcgaaatc gccccacgcg caggcaccgt cggacgcata ccaccagagg gtttttgcat
    15901 cgcccggaaa agaccagttg gcgctctcgt aagtggatat gggttctccg agcagcgcca
    15961 agacatccga cacgttcatc ccgattgcaa cagtggcaaa ggcagcatcg ctgtagccat
    16021 ccgcaaaacg ggtatcaatg tccggtttga aaataaattt gccctcaggt ggtgcgggga
    16081 acatcaccag aaagagaagg ccaacccaga tgccgcaaat tatcaccgct gagacgaaaa
    16141 ctggtgtcct tggttgagtc ttttgctttg ctatccacaa cgcgattgtc agggcaacag
    16201 gtccgagcat tccggcccct gccacgtaac ttgataatgg gctatgccag atgccaagtg
    16261 caatgcccag aataccgccc atagcggaaa tcccaatatg caggatcaga agccatgaaa
    16321 cgaattttat gtgattcatc tgttttgttg aaccataggc agccggcccc gccggcgcaa
    16381 tgccgaaagg gtggaactta catttcgccg ccctgctcgc gcaggaaggc cacgacttcg
    16441 tcgcgatggt ccgctgccgc aagccggcag gcggttttgc cgtaatcgtt gcgggcgttg
    16501 atgtcggccc cgcgctcgag caaaaagcgc acaatatcga ggtcgccgcg ttgggcggca
    16561 gccatcagcg gcgagcgccc agtacggcct ttggcattca catccgcatc tttttccagc
    16621 aacagttgga taatctccag gtgcccgcca atgctcgcca acacgagcgg tggattattt
    16681 tcatggccgc cgtcattcgt tccagcaccg tgcgccagca gggtttgcac cacctccaac
    16741 cggccattac gcgcggcggc catcaacggc aattgtccgt ttttgttttc ggtgttcacc
    16801 gcaatgccgc gggcgagcag aaaatccaca atttccacga agccgccgct ggccgccaaa
    16861 tggatggggg gattgccgtc ttcgccgcgc gtgtccagct ccgcgccgtg atcggcgaga
    16921 aactggacga tagccgtgta acccttgagc gtggcccaca tgagcggggt ccaaccgttt
    16981 tgattccggg cattgacgtt ggccccgcgc tccgtcagga actgcaccag ctccaagtgg
    17041 cccttgttgg ccactgccat gagcgccgtc cacccggttt tgttggggga atcaatcggc
    17101 acccccttgt tcaccaaatc ctcgatggcc accgtatccg tggcattggc ggccttgacc
    17161 aaggcctcgg tttcctgctc cttcatggtg accgcatagt accaaacccc gccccccacg
    17221 caagaaaacc ctcgttcaaa ccctcaatgc aggcggtact gaagaaagac tagagacgac
    17281 agacgacaga ctacagacta tagacaagag accagggaag gactctcgcc aagacgccaa
    17341 gagcgcaaag gaagacagag gacgccaaac tgtagccggc ctcgctgagg ccggtattgc
    17401 gaagaaggaa tatcgtgttc accgcgcaca gccgcagaaa gggctacagt ctaacgccct
    17461 aactttccgg tgcgacaccc ccgcaagggc cgggtacatg aggagccgat tgttaggcca
    17521 tctttttaac ccatgtcttc acgagctctg gtattcgaga aattacttcc ttgccgacga
    17581 gcacctcatc gacgtagccc atgtgaagca tgtctacaat tctgcaaaga tacgacttcc
    17641 cgcagtccca ccacgtgtag tgtgcgtcga tgatcaaata atggaggacg ttcatctttt
    17701 ctgtcaaaag aagttcctca atttccatgc catcgaggag agtctcgtac acgccatcgg
    17761 cgactcggct cccaaaggtg gttgtgtagt agtactcctt cacctcccac acggcgattg
    17821 ggttgatgac ggatgggaat gcgccatcca tgcgtcgggc aaaggtgcgc aggggtaatc
    17881 catttgtagc gattgtggca agtgctcggg gatcgtagtc acattggtgc tcgcccgcgt
    17941 tggcttcgac aagcatgttg accatgccag tgagataggc gggcgccttc ttcatcccct
    18001 tttgcttatt catggggagt gggcatgttg gcttgagttg gcgcttgagc ttggtgaact
    18061 cgatcttggc ctgtttcgaa tccatcaggt tcggttctac ctgcccatga agggtatctc
    18121 gtcgatgggt gaaataggca acaagtgttt tccagacggg tcgtccgtcg gggagcgcgg
    18181 tatccagcga ttttgggtcc agtttgagtt ttgcaagtgc agcctgaact tctgctttgc
    18241 tcgcgacagt gatttggccg ccagctgcgt agccgcattc ttgaccgatt agccgcacaa
    18301 gagcccagaa gctcttgggc agttggatga atctcttgtc aggtgtcatg attgggcaga
    18361 tactccttca gtagggcgtg tggcggaata cccagcgcgc gacatacagt gacggtttcg
    18421 acaaaagaga gcagccgttc gccagactcg tatttactga cgaatgactg gggcttatcg
    18481 agaattgctg caagggcttc ttgtgtaagc cccatttcgg tgcgcttttg gcgtaacagt
    18541 cggcgcaatg actcctcgtt cttatcatat cggttatgtt tcatgtatcc gaatatagga
    18601 tactcttgac gcgaatccca aaacgggata ttttgtggat atgaaggtga aagaagatac
    18661 gctggaccta ttttacggaa ctgagttcca gcacgttgcc ccatttagga cccagctact
    18721 gaaatggatc ggaaacaagc agcgtttcgc ccacaatatt gcatccttct tccctgccga
    18781 tattcagact tattttgagc ccttcttggg tagtggagcg gtccttgggg cgcttcaacc
    18841 gcctcgggcc attgcctctg acgcttgcgg gccattgatg gagatatggc aaaccctgaa
    18901 caatgatcct gacctgctgg ttcggtggta tcgtgccaga cataaagagt atgagcagca
    18961 cggcaagcct gatggctacg agcgtatcaa ggcccgatat aatgcgtctc ccaacggtgc
    19021 ggatctgctt ttcatctgcc gcagttgtta tgggggcgtt gtacgtttcc gtaaaaggga
    19081 tgggtatatt tcaacgccct gcgggattca tgaccccatt tcgccgaccg cattcgctga
    19141 aagagttcag atatggcgcc ggcgaacagc cggggcacgc tttctcaacg tggactttga
    19201 agaatcgatg gcgatggcgc agtcgggtga cttggtttac tgtgatccgc cgtacgtctg
    19261 tagtcagaca atcctgtatc gcgggcaaga tttctcgtta ggccgtctga tgaatacgat
    19321 tgccgactgc aagacgcggg gtgttcgtgt tgcgctcagt attgacggca agaagaaatc
    19381 gggtcgtgtc gaatgcgatc tagatattcc agacggactc tttgagcgcg aggtgtatgt
    19441 ggattgcggg cgcagtatgc ttcgacgttt ccagcgcgag ggcgagtcac ttgaggacga
    19501 ggtggtccat gaccgcctgc tgttgacgta ctgatttttc ccactagggt taacggtcag
    19561 aacggccaaa cagcccgccg gggattacaa ccccggccta caaaatccgg tttgtggaat
    19621 tgtaggccgc cgctgtgtcg gcggcgggca taaaaaagcc cccaccggga gggtgggggc
    19681 gggggagggg ggcgcttagg ggttgccggc ggcgcgcagg gcgttgagga cccgttcggg
    19741 agtgaacggg aactcgtgga gacgcacgcc agtggcgtta taaagcgcat tgccaatggc
    19801 gggcgcgggg ccgttgatgc aaatttccga aacggacttg gcgccgaagg ggccggtatc
    19861 ctcatacgat ggcacgagga tggatttgat cggcggcttg tcgcgcatcg aaaagatgtg
    19921 gtagtcggcg aaggaggcgt tggtcatgcg tccgttcttg ttgaacaggt actgctccgt
    19981 cagggcatag gaaatgccgt tgagggtgcc gccttcggtc tggccttcgc agagggccgg
    20041 attgatgggc gtgccgcagt ccaccgtggc gacgtaattg agcaccttga tgttgccggt
    20101 ccacgtgtcc acttcgattt ccgcaaagtg cgcggcgaag ggcgggggcg acttctgagt
    20161 gatgtgcgac gcggtgccga tgatctggta ctggtttttc tggtagagcg catagcagca
    20221 gatgtcgcca taggagactt gctgtttgcc gtccttgctc tggacgtggg catctttgca
    20281 gatgacctgg tcttcgggca ggttgagcat ctcggcgccg gtgtgcagga tttggcgctt
    20341 ggcatcggcg gcggccttgc gggcggcttc gccggagaga tacgtggtgg acgaagcgta
    20401 ggcgccgaca tcgaaggggg tcatatcggt gtcggaggaa tacacgatca tcttgtcggt
    20461 ggtcgtacag agttcctccg cggcaatctg ggccagcacg gtatcggaac cggtaccgag
    20521 gtccgtcgcg ccgacgagaa ggttgaagga accgtcctcg ttcatcttga tggacgcgcc
    20581 gcccatgtca atgtgcggga tggaggagcc ctgcatgagc gcggccatgc ccaggccgcg
    20641 gcggtagcgc ccggtcttac cgcgccaatc ggtgcggcgt tcccaaccga tggcggcagc
    20701 gccttgcgtg atacactcgc cgagggcgca ggagccgacg gtcatggcga cgccttcctt
    20761 gccttcgccg agggcttcaa acacggggga accttcaccg gtcttgatat ggttgagttg
    20821 gcggaactta agcggatcca tgccgatggc ttcggccatt ttatcaatga cggactccat
    20881 ggcgaagaac gcctgcgtgg cgccgaagcc gcgataggcc ccgccgcacg gcaggttagt
    20941 gtagtagacc ttgccgagga agtgcacgtt ttcgcagcgg tacagcggca gcaccttgga
    21001 gccacagcag gagaccacgg tcaggccgtg gccgccataa gcgccggtgt tgttccagca
    21061 atccatgctg atagcggtga tggtgccatc ctttttgacg ccggtgcgca ggcggaaacg
    21121 gatggggtgg cgggtgcgac cgtagcggaa gttttcctcg cgggtgagct gccagaagca
    21181 gggccgcccg gtacgcagcg cgaacatcgc cacgacatcc tccaacagga cttcctgctt
    21241 ggagccgaag ccgccaccga tgcgcggctt gatgacgcgg attttctgga cgggaatatc
    21301 caacgtttgc gccacgatgc gccggcagtg gaagggcacc tgcgtggagg tggtaatgac
    21361 gatgcgcccg gcggaatcca cctgcgccaa ggagacatag ggctcgatgg ggcaatgctg
    21421 cgcgtaagga gtttcaaaca cttcatcaaa ggtgaagtcg gcttctttca ggcctttttc
    21481 gacatcgcca aagtcgcagc caacgcgagc cacgaggttt ttcttgggct cgtagggaat
    21541 cggaatgggc acatgggctt cgggttcgtc gtggatgatg ggggcgcctt cggccatggc
    21601 ttcatcgagg gagaagacgg gcttgagctt ttcatacacc actttgattt tggccagcgc
    21661 ggccttggcc tgctcctcgg tttccgcggc cacggcggcc acgcggtccc cgacaaagcg
    21721 caccttggtg tcaaacatga aggtgtcgta gggcgaaggt tccgggaaac cctggccggc
    21781 agtggtatgc gggatgcgcg gcacgttgcc atgccacagc accgccttca cgccgggcat
    21841 tttttcggcg gcggaaacat ccatggagac gatccgcgca tgggggtgtg ggctccagag
    21901 cattttgacg atcagggcat cgcgcggcac gaagtcggcc acatacgacg gcttgcccag
    21961 cgccaactgc ttggcatcca ccttggtgac ggagtggttg acctgcttga aatttttcgt
    22021 tttttccagg ctcataataa cctcaggggt tcaggggtga tttcgctggt gaggggttag
    22081 gacgacttgg cggaaccctt gccggcttgt ttgcggtcga ggaccttttg cagggcatcc
    22141 cagatttttt cgtagccggt gcagcggcag aggttgccat ccatgtattc gcggacgaca
    22201 tccggcggat aagagtcctc ggccttcatc agcgcctcgg cggacatgat catgccgggg
    22261 atgcagaacc cgcattggac cgcgccggcc ttgatgagct cctgttggaa ctcggaaggc
    22321 tggtcgtggg tgccgacgct ctcaatggtg cggacttcgc ggttttcagc ctgcatggag
    22381 taaagcaggc aggcatagac cgcgcggcca tccagcagga cggtgcaggc gccgcaggtg
    22441 ccttggccgc agccgtattt ggtgcctttg tagccctcgc ggcggagcag atcgagcagc
    22501 ttctcgtccg cgcgggtgat gaaggttttg agttcgccgt tgagggtgaa cgtgacggtt
    22561 tcggggtgct tgatcattat tcagtcctcc cgcgggcaaa gctggcggcc cgctccaacg
    22621 catccaaaat ggtgacttca gccaagtggc gggaatagtc ggcggtcatg ccttcacgtc
    22681 cgcgccaagt gacatcttcg ccggccttgc tggcggcttc cttgaatacc gctggggtgg
    22741 cttcgcggcc gaccaaggcg gcttccaccg tcggcaaacg gcgggggaag ggaatagcgg
    22801 aacccgcggc catgcgggcc gacttgatct ggttgccatc cagttccagg aacgcggcga
    22861 tagtcacgtg agcaaaggcg gcatcggtgc gggtgacctt gatggaccca aacccgtggt
    22921 gcgcgtgcag cacgggaacc ttcaccgagg tcaggatgtt gccatccaaa aagcgcttgg
    22981 ccggctgggt cttgaaatat tcgtcggcct cgaattcttc ctcttggccg gaatagatca
    23041 ccatgcgcgc atccatggcc agcaggatca ccggcaggtc cgcccaaggg aacacgcggc
    23101 agatgttgcc gccgatggtg gaaatgttgc ggatttggtg ggtggaaatg cgccggcaaa
    23161 tttcgtgcat ggcccagcgc ggggcgcggt agcgctggat atcggccagc ggcgtggtgg
    23221 cgccgatgtt gtagtagttg ccttcctgtt tgatgaagga aagcccgagg cgggtaatat
    23281 ccaccgcggt cacgggggtg tggtcttgca tgaagtgcaa cgccgtgcca ccggcgagaa
    23341 tcatgccctt gggtcccagt tcccgcagat tgtcgcgggc ggcggccagt gaagctgggc
    23401 ggatgaagtc acataatttc atggatggtc tcctaaacgc agaggacaaa acaagcggcc
    23461 attatggagc agaagcgagt ggcccgaaag cacaaaaatg gcccaccggg ctgtatcgtt
    23521 ttactcagcc gcgggcgccg ccgagcaacg ccgcgcgcac cccggcgctg ttcttggcgt
    23581 gagccagcac ggtgatttgg tcgccgccct tcaattgcgt ggcgccgagc gggctgatga
    23641 cttcctcgcc gcgcgaaatc agcgtgatga ccgtatcggg gggcaggcgc aaatcctgga
    23701 tgcgcgggcc atccgtaccg atggattcgg gcaaatccac caccagcaaa tccatatcgc
    23761 tgggggccat ggtgatcagt tccaagctgt acagcggctc ttggcgggtg cgcgcggtca
    23821 gcaggcccag cgcgcgggcg cacaccccca gcgtggagcc ctgcaccagc accgagagca
    23881 gtaccgcgaa aaacaccaga ttgaaaattt cctggctttc cggcaggccg gcagcggcgg
    23941 gataggttgc cagaataatc ggcacggagc cccgcaggcc ggcccacgtc atgaacaacc
    24001 ggttcttgaa gcccaaaccc attttgggcg tgcccaagaa cacggcgatg ggccgcgaaa
    24061 taaacgtgat caccaaaaac aggatgaggc ccttgagcca aatatcgccc cattgccggg
    24121 gaaacaccaa caggcccagc agcacgaagg tgcagatatt ggcgatgctg gcaaaggcgg
    24181 aagaaaacgt cgccacgcca cgcttgtgga caaacgcatc gttgcccatg acatagcccg
    24241 cgataaacac cgcgagcatt ccactggccc gcaaggtttc cgtgacgccg taggtcaaca
    24301 gcaccacgcc gaggaaaagc acgtagtagt ggccgcggtc ctgcgggcgc agcacattga
    24361 acagccacag cgccgcccgc gccattagcc aaccgaatac cggtgccgcc gcaaacttcc
    24421 acgcaaactt gaggagcagg aacacaccct gcccatggcc caccaccaac gcattcacca
    24481 ccaccgtggt caacagcacc gccatcgggt cgttggccgc gctctccaac accagcgcgg
    24541 tggacagctt tttcggcaac gcaaagcgat gcaggatgga cagcgtggcc gcggcatcgg
    24601 tggaggaaat aatcgcggcg atcaaacagg cgtgcgagaa cggccaggcc agcagtttcc
    24661 acaagacgag gaatgtcgcg ccggcggtca gcagcacacc ccacacggcc agcccgccag
    24721 ccggcagcgc caccgcctgg aaatcgctgc gtttgatgcc aaagccgcct tggaacaaaa
    24781 tgaaaaccag ggcaaagttg gccacttgat tggccagcgt ggcattgtca aaatggaccc
    24841 attgcaggat gtcgctgccg aaaaacattc cgccgcccaa cgccaccaga atcaccggca
    24901 cgctccagcg gtccagccac actgcggcca gcaccacaaa aatcagaata gcgggggtta
    24961 tgacgtacac cagatccatg atggctccac caaaatcacg gcctcgcacc ctactgaccg
    25021 ccccggatct tggcaacgca aaatggctat ggcgcgcagg aagccgcagc ggcgcggaag
    25081 gccgtgcgga tttcgtcctc gccggggatt tggcggcggc gcatcaggac tttgccgttg
    25141 cagatggtgg tgtccacgca ttcgggggtg gcggcgtaga caagatcgct gatcaggtca
    25201 tgccctggga cgagcccagt gtgattcaaa tccaccagaa tgcaatccgc cgccttgccc
    25261 acggcgattt cgccggcatc cagccggaaa atgttgaagc cgtccgcggt ggcggtgcgg
    25321 tagatatcgg ccgcggcaca aacggtgggg tccatggcgt tgactttggc cagcagagcg
    25381 gcggtgcgca tctcggagaa catgtcgtag cggttgttgg tggccgcgcc atcggtgcc
//
LOCUS       DAOPNP010000020        24820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2542_length_24820_cov_8.609691, whole genome shotgun sequence.
ACCESSION   DAOPNP010000020 DAOPNP010000000
VERSION     DAOPNP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24820
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_24820_cov_8.609691"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKI64_03125"
     CDS             complement(<1..516)
                     /locus_tag="PKI64_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE00035.1"
                     /translation="MAHCLAQESRPPAPLISADSMAIYRGMDVGTAKPEAADRAAGPY
                     FGLDLVNPDQPFSVGDYLSAIHASTPEIVAAGGVPIVVGGTGLYVKALLEGLDPPASH
                     PAHRAAAEAILAAEGLPALQAAARALNPAEFAKLRDPENPRRVIRAYELLAAGHPPPP
                     PPPPRPPPPPPP"
     gene            complement(642..1100)
                     /gene="lspA"
                     /locus_tag="PKI64_03130"
     CDS             complement(642..1100)
                     /gene="lspA"
                     /locus_tag="PKI64_03130"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOE00036.1"
                     /translation="MTPLLVALLVIVLDQASKEWVRSVFSLHDTWPVIPGFFSLTYIR
                     NTGAAWGMFSGQNYALALLALVMLAVLVIFRRQILPPGRLHRVALGLLCGGIVGNLFD
                     RLRLDYVTDFLDFFYRGWHFPAFNIADAAICIGVGIYLLGALLAKPPPAA"
     gene            complement(1116..1685)
                     /locus_tag="PKI64_03135"
     CDS             complement(1116..1685)
                     /locus_tag="PKI64_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOE00037.1"
                     /translation="MAKKPVAKKAAPKKASSASRHLPPPPPPPPPARKAAKVRPFRKE
                     ELTYFRAQLHKELLRVQGNLEALAKDNLKRSPQESSGDISAHSTHMADQGTDNFDREL
                     ALNLVSGRQESLYEIEAAIRRVDEGTYGVCDTCGNGIERPRLKALPFAKKCVACQNAA
                     ERGRTKYRPFGGGLPLQQLAAEDSAEPVE"
     gene            1723..1995
                     /locus_tag="PKI64_03140"
     CDS             1723..1995
                     /locus_tag="PKI64_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00038.1"
                     /translation="MAFLIIGFFATAFFTTTFFVGAFFAAGFLTATFLATAFFGAGFL
                     ATIFLAAGFFTADFLADFLGAGRAGRALAGAFFAAFFLVAMVHLTR"
     gene            2144..2923
                     /locus_tag="PKI64_03145"
     CDS             2144..2923
                     /locus_tag="PKI64_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOE00039.1"
                     /translation="MKNRWRATLWIVLAGGLALAPARGADLAAEQQMALVRRSMPDVP
                     LELEAALQVVNPNGKTIKTVRAEARLVPQGDGRTAHYTLLDAFGGTLAEMTVALADGR
                     AEFAYAVGDPPQPAELPDLFGPIEGSVISWMELSFSYFWWPNPKIVGTEKVARRWDCQ
                     IIEIPCPPEYGAGWSYVRLWVAPAYNAVVRGEAWLDGRACKRFEVKSVKKLRQIYMIG
                     DLEVKDLRSGARARLKVGRMKMVSPDYSPEELEEFNAPISW"
     gene            2928..4397
                     /gene="xseA"
                     /locus_tag="PKI64_03150"
     CDS             2928..4397
                     /gene="xseA"
                     /locus_tag="PKI64_03150"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOE00040.1"
                     /translation="MSDLFAASPPAMPGEQPARQVWKVSELTRRIKGTLEGAFGSVWV
                     EGEISNLRRPASGHAYFTLKDATSQLRAVMFRSALAGVSLPLKDGLQVRGYGQITVYE
                     AAGDYQIVLRKLEAAGEGELMLRLEALKKKLAAEGLFALERKRPLPALPQHVGVVTSP
                     TGAAIRDILQVLKRRFGNLHVVVAPVRVQGAGAAEEIAAAIDLLNERGGLDVLIVGRG
                     GGSLEDLWCFNEEIVVRAIVRSRIPVISAVGHEIDWTLSDLAADVRAPTPSAAAEMVV
                     KSKAELERRVADAARRLGLGAQAAVLAWRNRLDRAARTPILRDPLQVVRQRQQTVDYL
                     GVRLHNALAGLPQLVRQRAETSAHRMELAMRLRLAESSRALQHAQAQLRLLNPKAVLT
                     RGYSLTRLPDGRLLRAASEVQPGMILKTELAAGAVLSTVTATEGETHDGKNSQNTKLR
                     GRTGAPGKNRGGDGKRQTRSGENGGGVRGGPEAGQTLLF"
     gene            4315..4488
                     /gene="xseB"
                     /locus_tag="PKI64_03155"
     CDS             4315..4488
                     /gene="xseB"
                     /locus_tag="PKI64_03155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOE00041.1"
                     /translation="MESGKLGLEKMVAAFEEGQKLVKLCSSKLNEVERKIELLVKKAD
                     GTVAAEPFPESES"
     gene            4584..5195
                     /locus_tag="PKI64_03160"
     CDS             4584..5195
                     /locus_tag="PKI64_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOE00042.1"
                     /translation="MAEELQHLIERIRKEGVESGAQAADALVAEAKQKAATIVAEAQK
                     QAKDLVTKAEQDAAAFAERGRATLQQAARDLLISIGGAVGDVVGGIVDAKVGAALSPE
                     LMAQMLLKLAEAYAQNGGEGGIVAMLGEADAAAVRAYFAKEYQDKLAAGIQIESSKEV
                     FKGFRVGAKGGQVFHDFTKEAIAESLAHFLRPELGQIVKKAAG"
     gene            5211..5765
                     /locus_tag="PKI64_03165"
     CDS             5211..5765
                     /locus_tag="PKI64_03165"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOE00043.1"
                     /translation="MYHYLVASLPTLTLGTAPPFSPADFRFKCQGVLSAPDLAELDLL
                     LAGQAAQGRTAFARAWAGGDAQIRNTAAKMRGGKLGVEAKSFLKSHPGYAVWLDKEVA
                     DALAKPSPLAREMGLDLARWKLADELVGADAYGLPAVLAFGVKIMLTARWAAMTDEAG
                     RERLEQLVSTLEENAAQSGAASFK"
     gene            5781..7514
                     /locus_tag="PKI64_03170"
     CDS             5781..7514
                     /locus_tag="PKI64_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOE00044.1"
                     /translation="MSENVGKIVGINGNLITVQFDQPVTQSEVGYARTGDIMLKSEVI
                     RIRGANAELQVFEDTTGLKVGDPVEFSGNLLAVELGPGLLAQVYDGLQNPLPKLAEQC
                     GFFLQRGTYLKALDRETKWDYTPQARRGDEVQAGDTLGTVPEGIFQHRIMVPFRLTGK
                     WKVVDTTPAGSYPVTHVVAKLKGERGEEVAVTMMQEWPVKVPIRAYAERLRPVEPLVT
                     KVRVIDTFFPVARGGVYCIPGPFGAGKTVLQQITSRHADVDIVIVAACGERAGEVVET
                     LREFPELTDPRTGRSLMERTLIICNTSSMPVAAREASVYTAVTIAEYYRQMGLNVLLL
                     ADSTSRWAQALREMSGRLEEIPGEEAFPAYLESVIAAFYERGGVVKLRDGQTGSVTIG
                     GTVSPAGGNFDEPVTQATLKVVGAFHGLNRERSDARRYPAIHPLDSWSKYPGVVDTQQ
                     VAQARRLLRQGNEVNQMMKVVGEEGTSIEDFVVYLKSEFLDAAYLQQNSFHDVDAATG
                     ADRQQYVFAKIHAILEAKVFFPDKDAARKWFLQLTSVTKDWNLVTMDSAEFKQLEQRI
                     DAMLAEVSDNA"
     gene            7507..8808
                     /locus_tag="PKI64_03175"
     CDS             7507..8808
                     /locus_tag="PKI64_03175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOE00045.1"
                     /translation="MHKIYHRIEQIAGNVITVRAEGVANGELAQVVGTLGTSLAQVIR
                     LDHDKVYLQVFAGSRGIATDGQVRFLGHKMDVSFTDALLGRVFNGSGQPRDNGPELDE
                     NRIAIGGPSVNPAKRIIPRKMVRTGIPMIDVFNSLVESQKLPIFAAAGEPYNELLARI
                     ALQAEVDIIILGGMGLKHDDYLQFRDTLEAHGALSRSILFIHTAADPTVECQMVPDIS
                     LAVAEQFALQNKRVLVLLTDMTSFADALKEIAITMEQIPSNRGYPGDLYSQLAARYEK
                     AVDFEGAGSITILAVTTMPGDDVTHPVPDNTGYITEGQFYLRNGRIEPFGSLSRLKQQ
                     VNGRTREDHRTVMNTMIQLYASFKETLEKQSMGFRMSAWDNRLLKYGERFEREMMDLS
                     VNIPLEAALDLGWQIMADCFEPEETGISAKLIAQFWPKTTA"
     gene            8818..9453
                     /locus_tag="PKI64_03180"
     CDS             8818..9453
                     /locus_tag="PKI64_03180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOE00046.1"
                     /translation="MGKVKNTKTELKAQRDALKRYRRYLPTLLLKKQQLQMEIRQVEQ
                     RVTEKRAEEEAARGALADWVKLYSDPLDYLSWLKVEAIDVEPGNIAGVAVRTLREIRF
                     QRTTPDLWATPAWLDEGLATLEQLFRLRIERRLLEEQLRLLAEELRTTSQRVNLFEKV
                     KIPECAENIRRIRIFLGDENTSAVVRSKIAKDKSIKKAAAPAAAVAGGSAA"
     gene            9462..11264
                     /locus_tag="PKI64_03185"
     CDS             9462..11264
                     /locus_tag="PKI64_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00047.1"
                     /translation="MKKLTVLCVAHDRKATLDALRELGVLHVTPVVPPAGSGLDVARE
                     KATGLRRLLEALPMAKDAKPTGHTFGEVVAEIGYLLDIRKDCQERLEYLQAEVARLEP
                     FGNFDPLQIKRLAAQNVWLKLYRAPAKGAPPMPEGVTAQKMGIRKNDAYWALFGCKPF
                     EWAGADEQRLPEDSLTELRAEIGRLQGALEATAKEMQALAGDRPLLERLLNEAEADVQ
                     LQEVRAGMGSAESVAYVQGFIPAEDVPQVKAVAATRGWGMLVVDPGPQDDVPVKLKPP
                     RWAAPIKAVFQGINILPAYNEADVSTVFMLFFSLFFAMIIGDAGYGLIFLGLTLWARN
                     KLPRNAVHLLVVTSVCTIIWGLITGTIFGMSPEILAKAGLNKIQVPFLTDPDKSAQNI
                     MGLCFLIGTIQLTIGHLWNVWELARSRQFKALEQFGWTLTTWFMFFLADDMVLKGHML
                     GYLGLSAETTAMLWRVLVYSLVAGIVLIVLFMMPPREIKDGWFNLALLPLSLVSNFTD
                     VVSYVRLYAVGTAGFAVANAFNNMIFGGDKPWWGLLLGAVLAFLAHTLNILLSTMGVL
                     VHGIRLNTLEFSNHKGISWSGSPYKPFAKPEMAT"
     gene            11317..11781
                     /locus_tag="PKI64_03190"
     CDS             11317..11781
                     /locus_tag="PKI64_03190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752258.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOE00048.1"
                     /translation="MDAATIEALGKAGGAAALGLAAVGSSIGAGLAGASAIGAWKKCY
                     AQSKAAPFILITFIGAPLSQTIYGMILMGWMVEVAKSATAAQNWATMLIGGIFGGLGI
                     GVSAWFQGYAGAAGADALAETGQGFGNYLMTLGVIETVALFVLVFIRSSFMG"
     gene            11805..12191
                     /locus_tag="PKI64_03195"
     CDS             11805..12191
                     /locus_tag="PKI64_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOE00049.1"
                     /translation="MNTNKRAGFTLVELLTVIAIIALLAALILGLAGNAQKKAARDRA
                     NAEITQLSSFVGDYLAQYGQVPQNLATLSNALKAANHSLTNLQDPWGMSYKYEASSKV
                     TFYLWSLGGDLSATGTNRAMWIGSAP"
     gene            complement(12202..12882)
                     /locus_tag="PKI64_03200"
     CDS             complement(12202..12882)
                     /locus_tag="PKI64_03200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE00050.1"
                     /translation="MKGLRGWFSMLLAVVLAGLLIGCGGGDDKISNPDPGSNDLNVIV
                     AFGDSITKGSECPCAAYPPRLAALTGKVVHNAGIGGSRATGGVGRTKQVINKYHPAFM
                     LILYGVNDVIHSYSRDAIIGALDRMVTICQENHVVPVLATYPMPIHGHRFFAGSTVRL
                     NQAIRDLAAARNIRCVDLEREFATGYDHSDPNHTRPIPDPSLYMPDGLHPNDAGTQVM
                     AMAFADLF"
     gene            13126..14259
                     /gene="alr"
                     /locus_tag="PKI64_03205"
     CDS             13126..14259
                     /gene="alr"
                     /locus_tag="PKI64_03205"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOE00051.1"
                     /translation="MARMINCDGSWVEVDLRVVRDNVAAIRAALGPTQLMAVIKSDAY
                     GHGLTPLAAALAGCGVRHFAVAYVAEAEAVRAVAPNAEMIVVLGATWPADVPRLLRSR
                     ITPAVVSQAHGEALAAAAVAAGGELPVHLKLDTGMGRLGFICPDELPTAAALTQLPGL
                     SVQGVCTHFAMVEPERKPTAARNQVEKFKQAVPMLEAAVGRRLYRHMSSSRAALLLPE
                     CDHDAVRIGIALYGYSASEAGRRFQTRPILSWKSRVMQVKRVPAGFPVGYYSSYRTSA
                     PTDLAVISCGYTDGYQRHLGNRGHVLIGGQRRPVVGRVSMNWVTADLGANSGVQPGDE
                     VVLMGIQGDQAIWADELAKHCSTIPYEILTGISRQIERRYIGG"
     gene            14275..14886
                     /gene="rdgB"
                     /locus_tag="PKI64_03210"
     CDS             14275..14886
                     /gene="rdgB"
                     /locus_tag="PKI64_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOE00052.1"
                     /translation="MKILIASHNAHKIQEIREIFDLPHVEWVSTAAFPELGEVVEDGD
                     TFEANATKKARELALATGLWALADDSGLEVAALDNRPGVYSARYAGEPGNHARNNARL
                     LQELAGVKNRAARFRCVAALSDPTGRVETVAGACPGRIIEELRGAQGFGYDPLFIPDG
                     HDRTFAEMGNEEKNKLSHRGRAMTLAKEKWGAIIAAELPRFPE"
     gene            complement(14904..15719)
                     /locus_tag="PKI64_03215"
     CDS             complement(14904..15719)
                     /locus_tag="PKI64_03215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOE00053.1"
                     /translation="MPPLDDLRLFLPIATALIALLVLGVAALFYLADGRRRATQSWSD
                     ALAHYAAVSPWRGRDVLLVILAMAAAQVIRHFLPERVWLDVICFQGVGVAVVLALAAR
                     KKQPFGAPITCGTALWQAALRWLALLPILWFSAFMWQGLLQLLGHATELQTAVQIFLE
                     THDPRQQLLFFALGVGLAPLAEELFFRGLLLPLLVRRWGPLPGLLVSALLFAVLHADL
                     GSLPALAIFAVGLALACARTGSLWVAVLMHALFNAANMALLLALARAGVLNSP"
     gene            complement(15719..16708)
                     /locus_tag="PKI64_03220"
     CDS             complement(15719..16708)
                     /locus_tag="PKI64_03220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE00054.1"
                     /translation="MTTTPFSLSVVIPVYNEEPNVAVLAKKVHDALSAFGRTYEIILV
                     DDGSRDASWERLCEAARTYPHFRLIRFRRNFGQTAAMSAGIDAAKHDVIVTLDADLQN
                     DPADIPLLLAEMERNDYAVVSGWRKDRKDPFINRRLPSILANGLISRITGVHLHDYGC
                     TLKAYRRNVLQGVRLYGEMHRFIPALCSWVGGEIAEVVVSHHPRVAGKSKYGIGRTFR
                     VILDLFTVKFLLRFTGGPMQLFGKIAFLFGGGAAIMLLIVLLERLFGSGGAEHLYLIK
                     RPFWVITPFMLLAFCMQFISMGLLAEVQIRTYHESQDKRIYAIRETFESPPAA"
     gene            complement(16760..18253)
                     /locus_tag="PKI64_03225"
     CDS             complement(16760..18253)
                     /locus_tag="PKI64_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE00055.1"
                     /translation="MQTNENFSAAARARNLAQMGTGNFDVLVVGGGIQGATIAWATAR
                     RGLRVALVEAADWGAGASANSLKIIHGGLRYLQHANLVRMRESIRARRRIIQLAPATV
                     EPLACIMPGRGWGLRNPLALLAALTANAILSADRNRGAPANRRLPAGRLISRRALEAA
                     FPLGIFRDESAGMVWYDAIALDIDRLTLAFVEAAANFGAVTANYAKATQWLRDGDKVV
                     GATVEDTLTGQRHEVRATHVVCSAGPGTLALSPNAGAPRWVKAINLVLKKPLVQDYAL
                     AIESKCEYRDSNALIQRGHRNFFVVPWQGGTMVGTLYVPYDPAKDSLPATAGEILELM
                     KQVNETGFLPPIQPGDICRVHSGAQAAGKQDTEPATQTQVLRAAPGLWVVQGVKYTTA
                     LETADDVADRLARGRRRPADEPLPWREPPQDLVALVAQAVDSEMAVRLEDVIMRRSHL
                     GFFGLPDEATMASCADAMARRLGWDVARREAEIAALRQAHSLPPLGE"
     gene            18473..19642
                     /locus_tag="PKI64_03230"
     CDS             18473..19642
                     /locus_tag="PKI64_03230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00056.1"
                     /translation="MKILLLAPQPFYEERGTPIAVNLLLRVLSARGATVDVLTYPVGA
                     DREHPGVTIHRTRRVPGIRKIPPGLSFAKLVCDAFMFFQAWGMVRRQRYDVVHAVEES
                     AFIARFLQRWYRVPFIYDMDSSLSRQIVDKHPWLGWLGRAMRWAEGRMIRAATAVIAV
                     CPALVEEAQSHGARQVYLLTDISLLQEVEPTPAEQALFPPTGAGCRFVYVGNLESYQG
                     IDLLVAAFHRLVTDPTSSPAQLIVAGGVPGDIARYEAMCRQLGVADRVRFIGPQPVDR
                     LGAMLALADVLVSPRTQGNNTPMKIYSYMASGKPLLATALPTHTQVLNDETACLAAPE
                     PAAFAEGMRRLAVDPELRARLGAAAQARAEAAHSWPAFQRTVNQIYDDLAARQGQ"
     gene            complement(19655..20641)
                     /locus_tag="PKI64_03235"
     CDS             complement(19655..20641)
                     /locus_tag="PKI64_03235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE00057.1"
                     /translation="MKILVTGGTGFTGKALVRRLLDDGHQVVALDYKEGLKTEELRQW
                     GAEVVIGTVTDRDCVRRCMQGVEIVQHLAAAFRELNVPESYYDHVNVDGTRICLEEAH
                     AAGVKKFIYCSTCGIHGNVKNPPTDEDHNIGPADYYQSTKYKAEPVCLDFMKKTGMPV
                     TILRPAAIYGPGDPERFQMIFKRVNRGRFPMFGSGKTLYHPLYIDNLTDAFVLAMDLH
                     KGNGEAYLIADEEYVEIKDLVQRVGKALNVDVQIPHYPIWPVIIAGHICEKVCKPLRI
                     TPPIFPRRVDWFRQNRAFKIDKAKRDLGYAPKVGLDEGLRRTAEWYRAEGLI"
     gene            complement(20734..21783)
                     /locus_tag="PKI64_03240"
     CDS             complement(20734..21783)
                     /locus_tag="PKI64_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HOE00058.1"
                     /translation="MNRTQIDIPSLVRVKTGAVGRVGIYARRHGFMEVALLHSADLHE
                     NLMNALCAGLQAENIAVLQRWPIREASFEQAREYFPQISARTRAIIGFGGGKALDVAK
                     YVAFLTRLPYLAVPTSLSNDGFCSPQSSLTLEGRRKSLPSAMPFGVVIDTEVCLAAPD
                     ILWHAGVGDLVAKLTAVADWKLAFHAVGTLVDDFAALLSDASVFQFIARPTHDLEGVR
                     LLGTALMLNGIAMAVCGSSRPASGSEHLISHALDSLSERPRMHGLQVGVATYLISLLQ
                     KQNSTTIADLFSKTGFWRTVAADPFRRDEWLEAIRRAPSIKQDFYTVLSSRDCLPEAE
                     AYLHNDPHLRACFVD"
     gene            complement(21780..22439)
                     /locus_tag="PKI64_03245"
     CDS             complement(21780..22439)
                     /locus_tag="PKI64_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOE00059.1"
                     /translation="MRTSPPKILVSDFDGTMTKYDFFDLALHDLPAASDHDYWQDFVA
                     GRLTHFAALQKIFANIRTDWAGVNDVIQRMELDPSLKDSVARLHAAGWEIIIASAGSE
                     WYIRRLLAQAGVELEVHANPGVFSPETGLVLSPPVDSPYYSPHTGIDKSAVMRAALAR
                     DPRAVFAGDGRPDLAPAQLVQADRLYACGWLADHLRQSNKVFHPFGTWSEIADHLLEQ
                     T"
     gene            complement(22490..22708)
                     /locus_tag="PKI64_03250"
     CDS             complement(22490..22708)
                     /locus_tag="PKI64_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE00060.1"
                     /translation="MMRNSSSMKIDPHANFGTRECPNCAVEVPANENRCPICGYEFPV
                     RGPLQRNLLWIVALLLLLLLWPLLRSLR"
     gene            22771..23844
                     /locus_tag="PKI64_03255"
     CDS             22771..23844
                     /locus_tag="PKI64_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE00061.1"
                     /translation="MSTKHLRNHLLGTCLLALATQLLAWGAPAGAGEAVTPVVNRDYL
                     PTLLGLIHGATQSIEFIQLECNDDRIMAQVEAALAAAVQRGVAVRGLLDDGVDFNAAA
                     VARLHTLGVDVQLDTPAKMTHAKVVIVDGRVVLLGSTNWTGNSMYNNNESNVRVADPA
                     IADYFARYLALLWADSAAEPELSPLVSGAIQTVINRQYFSAVLALFQTATQRIHVVMY
                     GINYSPKYAGSKVNQLVEALAAAAERGVAVSVTMDLSDYNHTLNNVNRAAKDYLMQAG
                     VTVFDDPLKTTTHAKVIVADDAVVIGSVNWGRDALEKRNEAAVLVRDRSVADFFADYA
                     QRPEVSAARRQLAAKKSPPEGGD"
     gene            complement(23907..24746)
                     /gene="tsf"
                     /locus_tag="PKI64_03260"
     CDS             complement(23907..24746)
                     /gene="tsf"
                     /locus_tag="PKI64_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOE00062.1"
                     /translation="MAEISAALVKELREETGVGMMECKKALVEANGDKAAAIKLLRES
                     GMAIAAKKASRTAKQGIIAAEIAADAQSGRMIEINCETDFVAKNDNFKAFVVEMLAAA
                     AGVPDNALAETAKAQVEAKIAEIGENIIARRNLTYQVQGTGAVVGYVHLGGKLGVLLE
                     VGFQKAASLANPAVKTLLSDLALHVTAVNPVCITRDQVPAETVQAEKEIYAKQVEGKP
                     ANIVDKIVAGKLEKFYGQICLVEQPFVKEQDKTVATILAETGKAVGDEFVIRRFARWQ
                     LGA"
BASE COUNT         4822 a         7181 c         8157 g         4660 t
ORIGIN      
        1 gggggggggg gggggggggg gccggggggg gggggggggg gggggggggt ggccagcggc
       61 cagcaactcg taggcgcgga tgacgcggcg cgggttttcg gggtcgcgca gtttagcgaa
      121 ttcggcagga ttcaacgcgc gggcggcggc ttgcagcgcg ggaagacctt cggcagccaa
      181 gatggcttcg gctgcggcgc ggtgggccgg gtggctggcg gggggatcca gaccttcgag
      241 cagcgccttc acgtagaggc ccgtgccgcc aaccacgatg ggcacaccac cggccgccac
      301 gatttccggg gtggaggcat gaatggccga gagataatcg ccgacagaaa aaggttgatc
      361 cggattaacg aggtccaacc cgaagtaagg cccggcggcg cgatcggcgg cttccggttt
      421 ggcagtgccg acatccatgc cgcggtagat ggccatggag tcggcagaaa tcaacggcgc
      481 gggtgggcgg gattcctggg ccaggcaatg ggccacggcg ctcttgcccg aggccgttgg
      541 gcccaccaaa aaataggccg ccagcggagt tttccacgct gtggaaaaat cggtggaatt
      601 ttttccccac tgtggaaaac ttttttccat agcgtggaaa atcaggcggc gggcgggggc
      661 ttggcgagga gtgcgccgag cagataaatg ccgacgccga tgcaaatggc ggcatcggcg
      721 atgttgaagg caggaaaatg ccagccgcgg taaaagaagt cgaggaagtc ggtgacgtag
      781 tcgaggcgga ggcggtcgaa caagttgccg acgattccgc cgcacagcag gccgagagcc
      841 acgcggtgca ggcggcccgg cggcaaaatc tggcggcgga aaatcaccag caccgcgagc
      901 atgaccagcg ccagcagggc gagcgcgtag ttttgaccgc tgaacattcc ccacgccgcg
      961 ccggtattgc ggatataggt caggctaaaa aagccgggaa tgaccggcca cgtgtcgtgc
     1021 aacgaaaaga cgctacgcac ccattccttg gacgcctggt ccagcacaat gaccagcagg
     1081 gcaaccagca acggggtcat ggggcggcgg gccgattatt ccaccggttc ggcggaatcc
     1141 tcggcggcga gttgctgcag gggtagaccg ccgccaaacg ggcggtattt ggtgcggccc
     1201 cgctcggcgg cgttctgaca ggccacgcac tttttggcga aaggcagggc cttgaggcgc
     1261 gggcgttcga tgccgttgcc gcaagtgtcg caaacaccgt aggtgccttc atccacgcgg
     1321 cggatggcag cttcgatttc gtagagcgat tcctgtcggc cagagacgag gttgagggcc
     1381 agttcgcggt cgaaattgtc ggtgccttgg tcggccatgt gggtggagtg ggcgctgata
     1441 tcgccggacg attcctgcgg tgagcgcttg aggttatcct tggccaatgc ctcgaggttg
     1501 ccttgtacgc gcaaaagttc tttgtggagc tgcgcgcgga aataggtcag ctcctctttc
     1561 cggaaggggc gcactttggc cgccttgcgg gcgggggggg gcgggggagg cggggggggg
     1621 aggtgccggc tggcggatga ggctttcttg ggggcggctt ttttcgcgac gggctttttg
     1681 gccatcgctt tttttgccac cggttttttg gcgacggttt gcttggcctt tttgatcatt
     1741 ggctttttcg ccacggcttt tttcacgacc acttttttcg tgggtgcttt cttcgccgcg
     1801 ggctttttga ccgcgacttt cttggctact gctttcttcg gtgctggctt cttggccaca
     1861 atcttcttgg ctgcgggttt tttcacagcg gattttttgg cggacttttt gggtgcgggg
     1921 cgcgcggggc gcgctttagc gggggccttt tttgctgctt tctttttggt cgccatggtg
     1981 cacctcacac gttagctgtt catcttgctt gcttccgaag acgcctccgg agctacacaa
     2041 aggttgcgct gtttatcatg gtgcgcgcgg gaaagtcaat ggccgaggcg cgcgtgggcg
     2101 aaaacatttg cggcaggcgg ggcgctcgcc taggatgggg cgcatgaaaa accgttggcg
     2161 ggcaacttta tggatcgtgt tggccggggg gctggcgctg gcgccggcgc ggggggccga
     2221 tctggcggcg gagcaacaaa tggcgctggt ccggcgctcg atgcccgatg tgccgctgga
     2281 attggaagcg gcgcttcagg tggtgaaccc caacggcaag accatcaaaa ccgtgcgggc
     2341 ggaagcgcgg ctggtgccgc agggcgatgg gcggacggcg cattatactt tattagatgc
     2401 cttcggcgga acgctggcgg agatgaccgt ggcgctggcc gacggccggg cggaatttgc
     2461 ctacgccgtg ggcgacccgc cgcagccggc ggagctgcca gacttgtttg ggccgattga
     2521 gggcagcgtc atcagttgga tggagttgag tttctcgtac ttctggtggc cgaaccccaa
     2581 gattgtcggc accgaaaaag tggcgcggcg ttgggattgt caaatcattg agattccctg
     2641 tccgccggaa tacggcgcag ggtggtcgta cgtccggcta tgggtagcgc cggcctataa
     2701 cgcggtggtg cgtggcgagg cttggctgga tggacgcgcg tgtaaacggt ttgaggtcaa
     2761 gtcggtcaaa aaactccgcc agatttacat gattggggat ctggaagtga aagacctgcg
     2821 gagtggcgca agggcgcggc tgaaggtggg ccggatgaag atggtttccc cggattattc
     2881 accggaggaa ttggaagagt tcaacgcgcc gatttcgtgg tgaagggatg agcgatttgt
     2941 ttgcagcatc accgccggcc atgccgggcg agcagcccgc gcggcaagtt tggaaggtgt
     3001 ccgagctgac gcggcgcatc aagggcacgt tggagggcgc atttggcagc gtgtgggtgg
     3061 agggcgagat ttccaatttg cggcgaccgg cgtcggggca tgcgtacttc acgctcaagg
     3121 atgccacgag ccaattgcgc gcagtgatgt tccgcagcgc cttggcgggg gtgagcctac
     3181 cgctgaagga tggcctgcaa gtgcgcggct acggacagat cacggtgtat gaggccgccg
     3241 gcgattacca gattgtgttg cgcaaactgg aggctgccgg cgaaggtgag ctgatgctgc
     3301 gtcttgaagc gctgaagaaa aaactcgcag cggagggctt gtttgccttg gagcgtaaac
     3361 gcccgctgcc cgcgctgccg caacatgtag gcgtggtaac ttcgcccacc ggcgcggcta
     3421 tccgcgatat tctgcaagtg ttgaaacgtc gtttcggcaa tctgcacgtg gtggtggcgc
     3481 cggtgcgtgt gcaaggggcg ggggccgcgg aagaaatcgc ggcggcaatt gatttgctga
     3541 atgaacgcgg cgggctggat gtgctgattg tggggcgggg gggcggcagc ctcgaagatt
     3601 tatggtgctt taatgaggaa atcgtggtgc gggcaatcgt tcgctcgcgg attccagtga
     3661 tttcggcggt cgggcacgag attgactgga cgttaagcga cttggccgcg gatgtccggg
     3721 cgccgacacc cagcgccgcc gccgaaatgg tggtgaagag caaggcggag ctggagcggc
     3781 gcgtggcgga tgccgcgcgg cggctcggcc tcggcgcgca ggcggccgtg ttggcatggc
     3841 gcaaccgcct cgaccgcgcg gcgcggacac ccatcctgcg cgatccgctg caagtggtgc
     3901 gccagcggca acagacggtg gactatctgg gcgtacggct gcacaacgcg ctggccggcc
     3961 tgccacaact ggttcgccaa cgagccgaga ccagcgcgca ccggatggaa ttggccatgc
     4021 ggttgcgcct cgcggaaagc agccgcgcgc tgcagcacgc gcaggcgcaa ttgcgcctgc
     4081 tgaaccccaa ggcggtgctg acccgcggct atagcctgac ccggctgccg gatggccgat
     4141 tgctgcgcgc ggccagcgaa gtccaaccgg gcatgatctt gaaaacagag ctggcagccg
     4201 gcgcggtgtt gtccaccgtg acggccaccg aaggagaaac ccatgacgga aaaaacagcc
     4261 aaaacaccaa gcttcgagga cgcactggcg cgcctggaaa aaatcgcggc ggagatggaa
     4321 agcggcaaac taggtctgga gaaaatggtg gcggcgttcg aggagggcca gaagctggtc
     4381 aaactttgct cttctaaact gaatgaggtg gaacgcaaga tcgagttatt ggtcaaaaag
     4441 gctgacggta cggtggcggc ggagccgttc ccggagagcg aatcgtaagc gtttcggcgc
     4501 ggcggcggtt ttgagattga aacgagcggc gggggtagtt atcctgccca tgcgtttagt
     4561 attttgtgga gcaaaggaaa accatggctg aagaactgca acatctgatc gaacgtatcc
     4621 gcaaggaagg ggtggagtcc ggagcccaag ccgccgacgc gctggtggcg gaagccaagc
     4681 agaaggcggc gaccattgtg gccgaggccc aaaagcaggc caaggatttg gtgacgaaag
     4741 cggagcagga tgccgcagcc tttgccgagc gcggccgggc cacgctgcag caggcggcgc
     4801 gcgatttgct gatcagcatc ggcggcgcgg tgggcgatgt ggtggggggc attgtggatg
     4861 ccaaggtcgg cgcggcgctc tcgccggagc taatggcgca aatgctgttg aaactggccg
     4921 aggcgtatgc gcagaacggc ggcgaggggg gcatcgtggc gatgctgggc gaggcggatg
     4981 ccgctgcggt gcgggcctac tttgccaagg aatatcagga caagctggcg gccggcattc
     5041 agatcgaaag cagcaaggaa gtgttcaagg gattccgcgt gggcgccaag ggcgggcagg
     5101 ttttccacga tttcacgaaa gaggccattg cggaatcgct ggcccatttc ctgcggccgg
     5161 agctggggca gattgtcaag aaggcggcgg gctaggactc accgatgggg atgtaccact
     5221 atttggtggc ctcgctgccg acgctgacgc tgggcacggc gccgcctttt tcgccggcgg
     5281 atttccgctt caaatgccag ggcgtgttga gcgcgccgga cttggcggaa ctggatttgc
     5341 tgctggctgg gcaggcggcg caagggcgca cggcattcgc gcgcgcgtgg gcgggcgggg
     5401 acgcgcaaat ccgcaatacg gcggccaaaa tgcgcggcgg caaactcggc gtggaggcca
     5461 agtcgttcct caagagccac ccgggctacg cggtgtggct ggacaaggaa gtggccgatg
     5521 ccctcgccaa gcccagcccg ctcgcccggg aaatggggct ggatttggcg cgctggaaat
     5581 tggccgacga gttggtcggc gcggatgcct acgggctgcc ggcagtgttg gcgttcggcg
     5641 tcaaaatcat gctgacggca cgatgggcgg ccatgactga cgaggccggc cgggaacggc
     5701 tggaacaact ggtttctacc ttggaggaaa atgcggccca aagtggcgcg gcaagtttca
     5761 aatagcgaag gcggtcgaag atgagcgaaa acgtgggtaa aattgtagga atcaacggca
     5821 acctgatcac ggtgcaattt gaccagccgg tgacgcagag cgaagtgggc tacgcgcgca
     5881 cgggcgacat catgttgaag tccgaggtca tccgcatccg cggcgccaac gcggaactgc
     5941 aagtgtttga ggacaccacc gggctgaagg tgggcgatcc cgtggagttc agcggcaacc
     6001 tgctggcggt ggagttgggc cccggcctgc tggcgcaggt gtacgacggt ctccaaaatc
     6061 cgctgccgaa gctggcggag cagtgcgggt ttttcttgca gcgcggcacc tacctgaagg
     6121 cgctggaccg cgaaaccaag tgggactata ccccccaggc gcgccgcggc gatgaagtgc
     6181 aggcgggcga tactctcggc accgtgccgg aaggcatctt ccagcaccgc atcatggtgc
     6241 cgttccggct gacaggaaaa tggaaagtgg tggataccac gccggcgggt tcctaccccg
     6301 tcacgcacgt ggtggccaag ctgaaagggg agcgcggcga agaagtggcc gtgaccatga
     6361 tgcaggaatg gcccgtcaag gtgcccatcc gggcctacgc cgagcgcctg cgcccagtgg
     6421 aaccgctggt gaccaaggtg cgcgtgattg acacgttttt cccggtggcc cgcggcgggg
     6481 tgtattgcat cccggggccg ttcggcgcgg gcaaaaccgt actgcagcaa atcaccagcc
     6541 gccatgcgga tgtggatatt gtgattgtgg cagcgtgcgg cgagcgcgcc ggcgaagtgg
     6601 tggaaacact gcgcgaattt cccgagctga ccgacccgcg cacggggcgc agcctgatgg
     6661 agcgcacgct gatcatttgc aacacgtcat ccatgcctgt ggccgcgcgc gaagcatcgg
     6721 tttatacggc ggtgacgatc gcggaatact accggcagat ggggctgaac gtcctgctgc
     6781 tggctgactc caccagtcgc tgggcgcagg cgctgcgcga gatgagcggc cgccttgagg
     6841 aaattcccgg cgaagaagcc ttcccggcct atctcgaatc ggtgattgcc gcgttctacg
     6901 agcgcggggg tgtcgtcaaa ctgcgcgatg gccaaaccgg ttcggtgacc atcggcggga
     6961 cggtctcgcc ggcgggcggc aactttgatg aacccgtcac gcaggccact ctgaaagtgg
     7021 tgggcgcctt ccacggcctg aaccgcgagc gctccgacgc gcggcgttat ccggccatcc
     7081 atccgttgga cagttggagc aagtatcccg gtgtggtgga tacgcagcag gtagcgcagg
     7141 cgcgccggct gttgcggcag ggcaacgaag tcaaccagat gatgaaggtc gtcggcgaag
     7201 aaggcaccag catcgaggat tttgtggtgt atctgaagag cgagttcctt gacgcggcct
     7261 acctgcagca gaacagcttc cacgacgtgg atgcggccac gggcgccgac cggcagcaat
     7321 atgtgttcgc caaaatccac gccatcctgg aggccaaggt gttcttcccg gacaaggatg
     7381 cagcgcgcaa gtggttcctg caattgacgt cggtgaccaa ggactggaac ctggtgacga
     7441 tggattcggc ggagttcaaa cagttggaac agcggattga cgcgatgttg gcggaggttt
     7501 cggacaatgc ataagattta tcatcgcatc gagcaaattg cgggcaacgt catcaccgtg
     7561 cgcgccgagg gcgtggccaa cggtgaactg gcacaggtgg ttggcacgct gggaacttcg
     7621 ctggcgcagg tgatccggct ggatcacgac aaggtgtatt tgcaggtgtt cgcgggcagc
     7681 cgcggcattg ccaccgatgg ccaagtgcgc tttctggggc acaagatgga tgtatccttc
     7741 acggatgcgc tgctggggcg cgtgttcaac ggctccggcc agccgcgcga caacggtccg
     7801 gaactcgacg aaaaccgcat tgccatcggc gggccgtcgg tgaaccccgc caagcgcatc
     7861 atcccgcgca aaatggtgcg cacgggcatc ccgatgattg acgtattcaa ctcgctggtg
     7921 gaatcgcaaa aactgccgat ttttgccgcg gcgggcgagc cctacaacga gctgctggcg
     7981 cgcattgccc tgcaagccga ggtggatatc atcatcttgg gcggtatggg gctgaagcac
     8041 gacgactacc tccaattccg tgacacgctg gaagcgcacg gcgcgctctc gcgctcgatt
     8101 ctgtttattc acaccgcggc ggacccgacg gtggaatgcc agatggtgcc ggatatctca
     8161 ctggcggtgg ccgagcagtt cgcgctgcaa aacaagcgcg tgctcgtgct gctgacggac
     8221 atgaccagct tcgccgacgc gctcaaggaa atcgccatca ccatggagca gattccctcc
     8281 aaccgcgggt atcccggcga cctctattcg cagttggccg cccgctacga aaaggcggtg
     8341 gatttcgagg gcgcgggctc cattaccatt ttggccgtca ccaccatgcc gggcgatgac
     8401 gtgacccacc cggttcccga taataccggc tacatcaccg agggccagtt ctatctgcgc
     8461 aacggccgca tcgagccgtt cgggtcgctc tcgcgcctga aacagcaggt caacggccgc
     8521 acgcgtgaag accaccgcac cgtgatgaac accatgattc agttgtacgc ctcgttcaaa
     8581 gagaccttgg aaaagcagtc catgggcttc cgcatgagcg cgtgggacaa ccgcctgttg
     8641 aaatatggcg aacgcttcga gcgcgagatg atggacctgt ccgtcaacat cccgttggaa
     8701 gccgcgctgg acctcggctg gcagatcatg gccgactgtt tcgagccgga agaaaccggc
     8761 atttccgcca agttgattgc gcaattctgg ccaaagacga cggcctagga cgcgggcatg
     8821 ggcaaggtca aaaataccaa gacggaattg aaggcgcagc gtgacgcgct caagcgctat
     8881 cgccgctatt tgccgacgtt gctgctcaag aagcagcaac tgcaaatgga aatccggcag
     8941 gtggaacagc gcgtgaccga aaagcgggcc gaagaagaag ccgcccgcgg cgcactggcc
     9001 gactgggtca agctctactc cgatccgctg gactacctgt cgtggttgaa ggtcgaagcc
     9061 attgatgtcg agccgggcaa cattgccggg gtggccgtgc gcaccctgcg cgaaatccgc
     9121 ttccagcgca ccacgccgga cctatgggcc acccccgcct ggctggatga ggggctggcg
     9181 accctggaac aactcttccg cctgcgtatt gaacgccgcc tgctggaaga acaattacgt
     9241 ctcttggccg aagaactgcg caccaccagc cagcgcgtca atttgttcga gaaggtcaag
     9301 attccggaat gcgcggaaaa tatccggcgc atccggattt tcctcggcga tgaaaacacc
     9361 agcgcggtgg tgcgttcgaa gattgccaaa gacaaatcca tcaagaaggc ggccgccccg
     9421 gcggcggccg tggccggagg atcagccgca tgatcgtccc aatgaaaaaa ttgacggtgt
     9481 tgtgcgtggc gcacgaccgc aaggccacat tggatgccct ccgcgaactt ggcgtgctgc
     9541 atgtgacgcc ggtggtgccg ccagcaggat cggggctgga tgtggcgcgc gagaaagcaa
     9601 ccggcttgcg ccgtttgctc gaagcgttgc ccatggccaa agatgccaag cccaccgggc
     9661 atacttttgg cgaagtcgtg gcagaaatcg gctacttgct ggatattcgc aaggattgcc
     9721 aggagcggct ggaatatctg caagcggaag tagcgcggct ggagccgttc ggcaatttcg
     9781 atccgctgca aatcaagcga ttggccgcgc aaaatgtgtg gctcaagttg taccgtgcgc
     9841 cggccaaggg cgccccgccc atgccggaag gcgtcaccgc gcaaaaaatg ggcatccgca
     9901 agaacgatgc atattgggcg ttgttcggat gcaaaccatt tgaatgggct ggcgccgatg
     9961 aacaacgttt gccggaagat tcgctcaccg aattgcgggc ggaaatcggc cgcttgcagg
    10021 gcgcgctcga ggccaccgcc aaggaaatgc aggcgctcgc cggtgaccga ccgttgctgg
    10081 agcggctgct gaacgaagcc gaagccgatg tgcagctcca agaagtgcgc gcgggcatgg
    10141 gcagcgcgga atccgtggcg tatgtccaag gatttattcc ggcggaagat gtgccgcagg
    10201 tgaaagccgt ggccgcgacg cgggggtggg ggatgctggt ggtggacccc ggcccgcagg
    10261 atgatgttcc ggtcaaactc aagccgccgc gctgggccgc gccgatcaag gctgtcttcc
    10321 agggcatcaa cattttgccg gcctataacg aggcggatgt cagcacggtc ttcatgctgt
    10381 ttttctcgct gttcttcgcc atgattattg gcgacgcggg ctacgggctg atcttccttg
    10441 gcctgacgct ctgggcgcgg aataagttac cgcgcaatgc cgttcacctg ttggtcgtca
    10501 ccagtgtgtg cacaataatc tggggcctga ttaccggcac cattttcgga atgtcgccgg
    10561 aaatcctggc caaagcgggg ctgaacaaga tccaagtgcc gttcctgacg gatcccgata
    10621 agtccgcgca gaacatcatg gggctgtgct tcctgatcgg gaccattcaa ctgaccattg
    10681 gccacttgtg gaacgtgtgg gagttggcgc gcagccgcca attcaaggcg ttggagcagt
    10741 tcggctggac gttgaccacg tggtttatgt ttttcctggc cgatgacatg gtgctcaagg
    10801 gccacatgct gggttacctg ggcctgtctg ccgagaccac ggccatgctg tggcgcgtgt
    10861 tggtctattc tcttgtggcc ggcattgtgc tgatcgtttt gttcatgatg ccgccgcgtg
    10921 aaatcaagga tggctggttc aacttggcgc tgctgccctt gagtctggtg agcaatttta
    10981 ccgatgtggt gtcgtatgtc cggttgtatg cggtgggcac ggcggggttc gcggtggcca
    11041 atgcattcaa caacatgatt tttggcggcg acaagccttg gtggggcctg ctgctcggtg
    11101 cggtactggc gttcctggcg catacgctca acatcctgtt gagcaccatg ggcgtactgg
    11161 tgcacggcat ccggttgaat acgctggagt tttccaatca caagggtatt tcgtggagcg
    11221 gctcgcccta caagccgttc gcgaaaccgg agatggcaac ctagacaaaa gaatgtaaaa
    11281 aatgggcggc aacgcccgga agcgaggaga gcaaacatgg atgcagcaac aattgaagca
    11341 ttgggcaagg caggcggcgc agccgcgttg ggtttggcgg cggtggggtc gtccatcggc
    11401 gccgggttgg ctggcgcttc ggccattggg gcgtggaaaa aatgctacgc ccagagcaaa
    11461 gccgcgccgt tcattttgat taccttcatc ggtgcgccgc tgtcgcagac gatttacggc
    11521 atgatcctga tgggctggat ggtggaagtg gccaagagcg ccaccgcggc ccagaattgg
    11581 gccaccatgc tcatcggcgg catcttcggc gggctgggca taggcgtttc cgcgtggttc
    11641 caaggctatg ccggcgcggc gggcgcggat gccctcgcgg aaaccggtca gggcttcggc
    11701 aactacctga tgacgctcgg cgtcattgaa accgtggcgc tgttcgtgct ggtattcatc
    11761 cgctcgtcct tcatgggcta gccgctgctg ccgccggaga caccatgaac acaaataaac
    11821 gcgccggctt tacgctggtg gaactgctga cggtgattgc catcatcgct ctgctggccg
    11881 cgctgatttt gggcttggcc ggcaatgcgc agaagaaagc cgcccgcgac agagccaatg
    11941 ccgagattac ccaactctcc tcgtttgttg gggattatct cgcgcagtat ggtcaggtgc
    12001 cacaaaatct ggccacgctg tccaatgcct tgaaggcggc caaccactcg ctgacgaatc
    12061 tgcaggatcc gtggggcatg agctataaat atgaagccag ctccaaagtg accttctatt
    12121 tgtggtcgct gggcggcgac ttgagcgcca ccgggaccaa ccgggcgatg tggatcggct
    12181 ccgcgccgta ggcgcggcgc gttagaacag gtctgcgaac gccatcgcca tgacctgcgt
    12241 accggcatcg ttcggatgca ggccgtccgg catgtagagc gacgggtccg gaatcgggcg
    12301 ggtgtgattc gggtcgctat ggtcatagcc cgtggcaaac tcccgctcca gatccacgca
    12361 ccgaatgttc cgcgccgccg ccaaatcgcg aatagcttga ttcagtcgca ccgtactgcc
    12421 ggcaaaaaac cggtggccat gaatcggcat gggataggtg gccagcactg gcaccacgtg
    12481 gttttcctgg caaatagtga ccatacggtc cagcgcacca ataattgcgt cgcggctgta
    12541 gctgtggatc acgtcattga ccccgtagag gatcagcata aacgccgggt gatacttgtt
    12601 gatgacctgt ttggtgcgtc cgacgccacc ggtggcgcgg ctgccgccga tacccgcgtt
    12661 gtgcaccact ttgccagtca aggccgccaa acggggcgga taggcagcgc aggggcattc
    12721 gctgccctta gtgatgctgt ctccaaatgc aacaatgacg ttgagatcgt tgctgccagg
    12781 atcgggattg gaaatcttgt catccccgcc accacaacca atcaacagcc cagccaacac
    12841 cacggccaga agcatcgaga accaaccacg caagcctttc atgcccactc cataatctct
    12901 actggatatc ttctttgatg aaaaagttta ccgcaggtta agtgcctgtc cagcgtaaag
    12961 atgccgcaat tggccttttc aagctgaaag gtgactgcac tggttgagtt tgcgggaaaa
    13021 gatgcgtgaa gtggctaata ctcatgatgt ggtgagcggc ttttgtaatc ccaacggatg
    13081 ggcggcggct gaaaaaggtg gcgcggggct agcggggtgt gtttaatggc gcgcatgata
    13141 aattgtgatg gttcctgggt tgaagtggat ttgcgtgtcg tgcgcgacaa tgtggctgcc
    13201 attcgcgccg ccttggggcc gacgcagttg atggcggtga tcaaatcgga tgcctacggg
    13261 cacgggctga cgccgctggc ggcggcgctg gcgggctgcg gcgtacggca ttttgcggtg
    13321 gcctatgtgg cggaagctga agccgtgcgc gcggttgcac cgaatgccga aatgattgtg
    13381 gtgctgggtg cgacttggcc tgccgatgtg ccacggctgt tgcgctcccg gatcacgcca
    13441 gcggtggtgt cccaggccca cggcgaggcg ctggctgcgg ctgccgtagc agcgggcggt
    13501 gagttgccgg tgcatctcaa actggatacc ggcatggggc gcttgggatt tatctgcccc
    13561 gatgaactgc cgacggcggc ggcgttgacg cagctccccg ggctttccgt gcagggagtc
    13621 tgtacccact ttgcgatggt ggaaccggaa cgcaagccaa ccgcggcccg caaccaggtg
    13681 gaaaaattca aacaggcggt gcccatgctg gaggcagcgg tggggcgacg gctttatcgc
    13741 cacatgtcga gcagccgcgc ggcgctgctt ttgccggaat gcgatcatga tgccgtgcgc
    13801 attggtattg cgctctatgg ctacagcgcg tcggaagcgg gccgacgttt tcaaacccgc
    13861 ccgattttga gctggaaatc ccgcgtgatg caggtcaagc gagtgccagc gggatttccg
    13921 gtgggttatt acagcagcta tcgcaccagt gcgcccaccg atttggcggt gatcagttgc
    13981 ggctataccg atggctatca gcggcatctg ggcaaccggg ggcacgtgct gattggcggt
    14041 caacgccgcc ccgttgtggg gcgtgtcagc atgaactggg ttaccgccga cttgggcgcg
    14101 aatagcggtg tgcaacccgg cgatgaagtg gtcttgatgg gcattcaggg agaccaagcc
    14161 atctgggcgg atgaactggc caaacattgc agcactattc cgtatgaaat cctgacgggc
    14221 atttcccgcc agatcgaacg ccggtacatc ggcggctaaa ataaggaact ccccgtgaaa
    14281 atcttgattg cctcgcacaa cgcccacaaa atccaggaaa tccgcgaaat ctttgatctg
    14341 ccgcacgtgg agtgggtatc cactgccgct ttccccgagt tgggcgaagt ggtcgaggat
    14401 ggcgacacct tcgaggccaa cgccaccaag aaagcgcgtg aactggccct ggctaccggt
    14461 ctctgggcgt tggcggatga ttccggtttg gaagtggccg cgctcgacaa ccggccggga
    14521 gtgtattccg cgcgctatgc cggcgaacct ggcaatcatg cccgcaacaa cgctcggctg
    14581 ctgcaagaat tggccggcgt gaaaaaccgc gcggcgcgtt tccgttgcgt ggcggcgttg
    14641 agcgacccca ccggtcgcgt ggaaaccgtc gcgggtgcct gtccggggcg gatcatcgaa
    14701 gaactgcggg gggcgcaggg gttcggctat gacccgctat tcattcccga tggccacgac
    14761 cggacctttg ccgagatggg caacgaagaa aaaaacaaat taagccatcg tggtcgcgcc
    14821 atgacgctgg ccaaagaaaa gtggggcgcg atcattgcgg ctgagttgcc gcgtttccca
    14881 gaataacact tcgtcggtgg gcgtcatggg ctgttcaaaa cgccggcgcg cgcgagcgcc
    14941 agcagcagcg ccatattggc cgcgttgaac agcgcgtgca tcagcacggc tacccacaac
    15001 gaccctgtgc gcgcgcaagc cagtgccagc cccaccgcaa aaatagccag cgccggcagc
    15061 gagccgaggt ctgcgtgcaa caccgcgaag agtagcgcgc tcaccagcaa gccgggcagg
    15121 gggccccacc ggcgtaccag cagcggcaac agcaggccgc ggaaaaataa ttcttccgcc
    15181 agcggcgcca aacccacccc aagggcaaaa aagagcagtt gttggcgcgg gtcatgggtt
    15241 tctaaaaata tctgaacggc ggtttgcaac tccgtggcgt gacccagcag ttggagcaac
    15301 ccctgccaca tgaaagcgct gaaccacaga atgggcagca gggccagcca gcgcagtgcg
    15361 gcctgccaaa gcgccgtacc gcacgtgatg ggtgcgccga atggttgctt tttacgcgcg
    15421 gccaaggcca aaacaactgc cacgcccacg ccttggaaac agatgacatc cagccagact
    15481 cgctccggca aaaaatggcg aatgacctgc gctgcggcca tcgccagaat gacgagcagc
    15541 acatcccgcc cacgccacgg gctgacggcg gcgtaatggg ccagggcatc gctccaagat
    15601 tgcgtggcgc gtctacggcc atctgccaaa taaaaaagtg cggccacgcc cagtactaga
    15661 agggcaatca gcgccgtcgc gatggggagg aacaggcgca gatcgtcaag cggcggcatc
    15721 aggcagcggg cggcgactca aacgtctcac ggatcgcgta aatacgcttg tcctgcgatt
    15781 catgataggt gcgaatctgg acttccgcca gcaggcccat cgagatgaat tgcatgcaaa
    15841 aggccagcag catgaaaggc gtgatgaccc agaacggccg cttgatcagg tagagatgtt
    15901 ccgcgccgcc actgccgaac aggcgctcca gcagcacaat caggagcatg atggccgcgc
    15961 cgccgccgaa caggaaggcg atcttcccga ataactgcat ggggccaccg gtgaagcgca
    16021 gcaggaactt cacggtgaac aaatccaaaa tcacccggaa ggtgcgcccg atgccgtatt
    16081 tggatttgcc agccacgcgc ggatggtggc tgaccaccac ttcggcaatc tcaccgccca
    16141 cccacgaaca cagcgcaggg ataaaccggt gcatttcgcc atagagccgg acgccttgca
    16201 gcacattgcg gcggtaggcc ttcaacgtgc agccgtaatc gtgcaaatgc acgccggtga
    16261 tgcgcgaaat cagcccgttg gccaaaatgg acggcagccg tcggttgatg aaggggtcct
    16321 tgcggtcttt gcgccagcca gataccacgg cgtagtcatt gcgctccatt tccgccagca
    16381 gcaggggaat gtcggccggg tcgttttgca ggtcggcatc caaggtgaca atgacatcgt
    16441 gcttggcggc atcaatgccg gcggacatcg ccgcggtttg gccgaaattg cgccggaacc
    16501 ggatcagccg gaagtgggga tacgtccggg cggcttcgca taaccgctcc cacgaggcat
    16561 cgcgcgaccc gtcatccacc agaatgatct cgtaggtgcg gccaaacgcg gaaagtgcat
    16621 cgtgcacttt tttggcgagg acggccacgt tgggttcctc attataaacc ggaatgacaa
    16681 ccgacaggct gaacggagtt gtagtcatgc gcgcagtatg aaacagcccc cggggtattt
    16741 caagcgggct ttggccgggt tattcaccga ggggcggcag gctatgggct tggcgcaggg
    16801 ctgcgatttc cgcttcgcgg cgcgcaacat cccagcccaa acgccgagcc atggcatccg
    16861 cgcacgaagc catcgtagcc tcatccggca ggccaaagaa ccccagatgc gagcgccgca
    16921 tgatgacatc ttcgagccgt acggccattt cggaatccac cgcttgggcc accagggcca
    16981 ccaaatcctg cggcggctcc cgccacggca gcggttcatc cgccggccga cggcgaccgc
    17041 gcgcgaggcg gtcggccacg tcatcggcgg tttcgagcgc agtggtatat ttcactccct
    17101 gcaccaccca gaggccgggt gccgcgcgca acacctgggt ttgagtggcg ggttcggtgt
    17161 cctgcttgcc ggcggcttgc gccccagaat gcacccggca aatatcgccc ggctgaatcg
    17221 gcggcaggaa gccggtttca ttgacctgtt tcatcaactc caggatttct ccggcggtgg
    17281 cgggcaaaga atctttggcg gggtcatacg gcacatacaa cgtgccgacc atcgtgccgc
    17341 cctgccacgg caccacaaaa aagttgcggt gcccgcgctg gatcagcgcg ttggagtcgc
    17401 gatattcaca cttgctttcg atggccagcg catagtcctg aaccaagggc tttttcagca
    17461 cgaggttgat ggccttgacc cagcgcggcg cgccggcgtt gggcgagagc gccaacgtgc
    17521 ccggcccggc ggaacacacc acgtgagtgg cgcgcacctc gtgccgctgg cctgtcagcg
    17581 tgtcttccac ggtcgcgccg acgaccttgt cgccatcgcg cagccattga gtggctttgg
    17641 cataattggc ggtgacggcg ccaaagtttg ccgcagcctc gacaaacgcc agcgtcaggc
    17701 ggtcaatgtc cagcgcaatc gcgtcatacc agaccatgcc tgccgactca tcgcgaaaaa
    17761 tgccgagcgg gaatgctgct tccagcgcgc ggcgcgagat cagccgcccc gccggcaaac
    17821 ggcgattggc cggcgcacct cggttacggt ccgcgctcaa tatggcgttg gctgtcagcg
    17881 cggccagcag cgccagcgga ttgcgcaggc cccagccgcg ccccggcatg atgcaggcga
    17941 ggggctcgac ggtggctggc gccagttgga tgattcgtcg ccgcgcgcgg atggattcgc
    18001 gcattctcac caagttggcg tgctgcagat agcgcaggcc accgtggatg attttcaaac
    18061 tgttggcgct ggctcccgcg ccccaatcgg ccgcttccac caaagccacg cgcaggcccc
    18121 gccgggccgt ggcccacgcg atggtggcgc cttgaatgcc gccacccacc acaagaacat
    18181 caaagtttcc cgtgcccatt tgcgccaagt ttcgggcgcg ggccgccgcc gaaaagttct
    18241 cgtttgtttg catcgtttgc ttcatccatg ttttcgggcc ggcgcgcggg ttatgcttac
    18301 gcgggcggcc cgatttctac ttgctttcaa gtgcctgaac ggtaacttga acggcattga
    18361 ttagccacca aaaaatccat gatgcccggc ctcgggatgg ccgcccgccg acttccccgg
    18421 cggtgcgcgg tcgcgccggg cgacgcgggc ccccgccagc cgggaaagaa taatgaagat
    18481 actcctgctt gctcctcaac ctttttacga agaacgcggc acgcccatcg cggtgaatct
    18541 gttgctgcgc gtgctctccg cccgcggcgc gaccgtggat gtgctgacgt atcccgtggg
    18601 ggccgaccgc gagcaccccg gcgtgacgat tcaccggacg cggcgcgtgc cggggatccg
    18661 gaagattccc ccgggcctct cgttcgccaa gttggtgtgc gatgccttca tgtttttcca
    18721 ggcgtggggc atggtgcgcc gccagcggta tgatgtggtc cacgccgtgg aagaatccgc
    18781 attcatcgcg cgctttttgc aacggtggta tcgcgtgccg ttcatttatg acatggattc
    18841 atcgctctcg cgccagattg tggacaagca tccgtggctg ggctggctgg gccgcgccat
    18901 gcggtgggcg gagggccgaa tgatccgcgc cgccacggcg gtgatcgcgg tctgcccggc
    18961 cttggtggaa gaagcccaaa gccatggtgc gcgccaagtc tatttgctga cggatatttc
    19021 gctactgcaa gaagtcgagc ccacgcccgc ggagcaagcg ctgtttccgc cgaccggcgc
    19081 cggttgccgt ttcgtctatg tgggcaatct ggaatcttat caaggcattg atttactggt
    19141 ggccgcattc cacaggttgg tgaccgatcc gaccagctcg ccggcgcagt tgatcgtggc
    19201 cgggggtgtc ccaggagata ttgcccgcta cgaagccatg tgccgccagt tgggggtggc
    19261 cgaccgggtg cgcttcatcg gtccgcaacc ggtggaccgc cttggcgcca tgctggcgct
    19321 ggccgatgtg ctcgtttccc cgcgcacgca aggcaacaac acgcccatga agatctattc
    19381 ctacatggct tccggcaaac cgctgctggc cacggcgctg cccacgcata cccaggtgct
    19441 gaacgacgaa accgcctgcc ttgccgcgcc ggaacccgcc gcgttcgcag aaggaatgcg
    19501 ccgtttggca gtggaccctg aattgcgcgc ccggctgggc gccgcggcgc aagcgcgcgc
    19561 cgaagctgca cattcctggc cggccttcca gcgcacggtc aaccaaatct atgacgattt
    19621 ggccgcgcgc caaggccagt agggggcggc ccgtttagat caggccttcc gcgcgatacc
    19681 actcggcggt gcggcgcagg ccttcatcca gccccacctt gggcgcgtag cccaaatccc
    19741 gcttggcctt gtcaattttg aaggcgcggt tctggcggaa ccagtccacg cggcgcggga
    19801 aaatcggcgg cgtgatgcgc aggggcttgc acactttttc gcaaatatgc ccggcaataa
    19861 tcacgggcca aatcgggtag tgcggaatct ggacatccac attcaacgcc ttgccgacgc
    19921 gctgtaccaa gtccttgatt tccacgtatt cttcatccgc gatcagatag gcctcgccgt
    19981 tgcccttatg caaatccatg gccagcacga aggcatcggt cagattgtct atgtagagcg
    20041 ggtggtagag cgtcttgccg ctgccgaaca tggggaagcg gccgcggttg acgcgcttga
    20101 aaatcatttg gaagcgctcc gggtcgccgg ggccatagat ggccgccggg cgcaggatgg
    20161 tcaccggcat tccggttttc ttcatgaagt cgaggcacac cggctcggct ttgtacttgg
    20221 tgctctggta ataatcggcg gggccgatgt tgtgatcttc gtccgtgggc gggtttttca
    20281 cattgccgtg aatgccgcac gtggagcagt agatgaactt tttgacgccg gcagcgtggg
    20341 cctcttccag gcagatgcgt gtgccgtcca cgttgacgtg gtcataatag ctttccggaa
    20401 cgttcagttc gcgaaatgcg gcggccaagt gctgcacaat ttccacgccc tgcatgcagc
    20461 ggcgcacgca gtcgcggtcg gtcacggtgc caatgaccac ttccgcgccc cattggcgca
    20521 actcttcggt tttgaggcct tccttataat ccagcgcgac cacctgatgc ccgtcatcca
    20581 gcagccgtcg taccaacgct ttgcccgtaa atcccgtgcc acccgtcacc aaaatcttca
    20641 tttcagcctc tcttgttgcc tttgcgaata atgggccgac tctagcgaac ccgccacggg
    20701 caacgcaagg ctgcattccg gccgggcgcc ggtttaatcc acgaaacagg cccgcaaatg
    20761 cggatcgtta tgcagatagg cttccgcttc gggcaggcag tcgcgcgaac tcaagacggt
    20821 ataaaaatcc tgcttgatgc ttggcgcgcg gcggatggct tccaaccatt cgtcgcggcg
    20881 gaaggggtct gccgccacgg tgcgccagaa gccagttttg ctgaacaagt cggcaatggt
    20941 ggttgaattc tgcttttgga gcaggctgat gaggtaggtc gccacgccga cttgcaggcc
    21001 gtgcatccgc ggacgttcac tcaatgaatc cagcgcgtgc gaaattaaat gttcgctgcc
    21061 gctggccggc cgcgaggagc cgcacaccgc catcgcgatg ccgttcagca taagcgccgt
    21121 gcccagcaaa cgcacgcctt cgagatcgtg cgtgggccgc gcaataaact gaaacaccga
    21181 ggcatcggaa agcagcgcgg caaaatcatc caccagcgtg cccaccgcat ggaacgccaa
    21241 cttccaatcc gccacggcgg tcagtttggc taccagatcg cccacgcccg cgtgccacaa
    21301 gatgtccggc gccgccagac aaacttccgt atcaatcacc acgccgaacg gcatggccga
    21361 aggcagcgac ttgcgccggc cttccagcgt caaactcgat tgcgggctgc aaaagccgtc
    21421 gttggacagc gaggtcggca ccgccaaata cggcagccgg gtcaggaagg ccacatactt
    21481 ggccacatcc agcgccttgc cgccgccgaa accgataatg gcgcgggtgc gcgcggagat
    21541 ttgtggaaaa tattcccgcg cctgctcgaa ggaagcctcg cggatgggcc agcgttgtaa
    21601 cacagcgata ttctccgcct gcaaaccggc gcacagggca ttcatcaggt tttcgtgcaa
    21661 atcggcgcta tgcagcagcg ccacctccat gaagccatgc cgccgcgcgt aaatccccac
    21721 gcggccgaca gccccggtct tcacgcgcac caacgatgga atatcaattt gcgtccggtt
    21781 catgtttgct ccagaaggtg gtcggcaatc tccgaccatg tgccaaacgg atgaaagacc
    21841 ttgttgcttt gccgcaaatg atccgccagc cacccgcagg catacaagcg gtccgcctgc
    21901 accagttggg cgggggccag atcggggcgg ccatcgccgg cgaacacggc gcgcgggtcg
    21961 cgcgccaatg ccgcgcgcat cacggcggat ttatcaatgc cggtgtgggg gctgtaatag
    22021 ggcgaatcca cgggcggcga aagcaccagc cccgtctccg gtgaaaacac gccgggattc
    22081 gcgtggactt ccaattccac gcccgcctgc gccagcagcc ggcggatgta ccactcgctg
    22141 ccggcggagg cgatgatgat ctcccagccc gccgcgtgca gccgcgccac cgagtccttc
    22201 agcgagggat ccaactccat gcgttgaatg acgtcattca cgcccgccca atcggtgcgg
    22261 atgttcgcga agattttttg caacgcagca aagtgggtga gtcgcccggc aacaaaatcc
    22321 tgccagtagt catgatcgct ggcagccggc aggtcgtgca gggccaaatc aaagaagtca
    22381 tatttggtca tcgtgccgtc gaaatccgac accagtattt tgggagggct tgtccgcatt
    22441 ggggcagtat atcacaaaac gtgggtctgt gccgcggcgg catcgcggat cagcgcaacg
    22501 acctcagcaa tggccacagc agcaggagca acagcagagc cacgatccac agcaaattgc
    22561 gctgcaaagg gccacggacg ggaaactcat agccgcaaat ggggcagcgg ttttcattgg
    22621 ctggcacttc caccgcgcaa ttggggcact cgcgcgttcc gaagttggcg tgcggatcaa
    22681 tcttcatgct ggaactattg cgcatcattt gccggatagc aagcagcggg cagcttgtgc
    22741 ggttgcgcca atgtgcgcta cgctccggcc atgagcacca aacatctacg aaaccatttg
    22801 cttggcacgt gcctgctggc gttggcgacg cagctgctgg cctggggcgc acccgctgga
    22861 gcgggggagg cggtcacgcc agtggtgaac cgcgactatc tgccgaccct gttgggcttg
    22921 atccatggcg cgacgcaatc cattgagttc atccaattgg aatgcaacga tgaccgcatc
    22981 atggcgcaag tggaggctgc cttggcggct gctgtgcagc gcggcgtggc ggtgcgtggt
    23041 ttgttggatg acggcgtgga ctttaacgcc gccgccgtgg cccgcttgca cacgctgggt
    23101 gtggacgtcc aactcgacac ccccgccaaa atgacgcacg ccaaggtggt gattgtggat
    23161 ggccgcgtgg tgttgttggg ctcgaccaac tggacgggca actccatgta taacaacaat
    23221 gaatccaacg tgcgggtggc ggacccggcc attgccgatt attttgcgcg ctacctggct
    23281 ctcttgtggg ccgacagcgc cgccgaaccg gaactatccc cactggtttc cggcgccatc
    23341 cagaccgtca tcaaccgcca atatttctcc gcagtcctgg cgttgttcca gacagccacg
    23401 cagcgcattc acgtggtgat gtacggtatt aattattcgc cgaaatacgc cggcagcaaa
    23461 gtcaaccaac tggtcgaggc cttggcggcc gccgccgaac gcggcgtggc ggtcagcgtc
    23521 accatggact tgagcgacta caaccacacg ctgaacaacg tcaaccgcgc agccaaggac
    23581 tatctgatgc aagcgggcgt gacggtcttt gatgatccgc tcaaaaccac cacccacgcc
    23641 aaagtgattg tggcggacga cgccgtggtg atcggctccg tgaactgggg gcgcgatgcc
    23701 ctcgaaaaac gcaacgaagc cgctgtgctg gtgcgcgacc gctccgtcgc ggattttttt
    23761 gctgattacg cgcagcgccc tgaagtcagc gccgcccggc ggcaactggc ggcaaaaaaa
    23821 tccccgcccg aaggcgggga ctaattgagt tttccacggt gtggaaaaaa gttttccaca
    23881 ctgtggaaaa gtgcggcgcg cggcgtttag gcaccgagct gccagcgggc aaagcggcgg
    23941 atcacaaact catcgccgac ggccttgccg gtttcggcaa ggatggtggc gacggtcttg
    24001 tcctgctcct tcacgaaggg ttgctcgacg aggcaaatct ggccatagaa cttctccagt
    24061 ttgccggcga cgattttatc cacgatgttc gcgggcttgc cttcgacctg cttggcgtag
    24121 atttcctttt cggcctgcac ggtttcggcg ggaacctggt cacgtgtgat gcacaccggg
    24181 ttgaccgcgg tgacgtgcaa cgcgaggtcg gaaagcagcg tcttgacggc gggattggcc
    24241 agcgaagcgg ctttttggaa gccgacttcc agcagcacgc cgagcttgcc gcccaagtgg
    24301 acgtagccga cgaccgcgcc cgtgccttgc acctggtaag tcaggttgcg gcgggcaatg
    24361 atgttctcgc cgatttccgc gatcttggct tcgacttgcg ccttggcggt ttcggccaac
    24421 gcgttgtcgg gcacgccagc ggcggccgcg agcatttcca ccacgaacgc cttgaagttg
    24481 tcgttcttgg ccacgaaatc agtttcgcag ttgatttcga tcatgcggcc ggactgggca
    24541 tcggccgcga tttcggcggc gataatgccc tgcttggcgg tgcgggaggc tttcttggcg
    24601 gcaatggcca tgccggattc gcgcagcagt ttgatggccg cggctttgtc gccgttggct
    24661 tccaccaatg cctttttgca ttccatcatg ccgacgccgg tttcttcgcg tagttctttg
    24721 accagggctg cagaaatttc tgccatgggg tattcctttc tattgggggg gggggagggg
    24781 gagcggggcc ggccggacac acaccggtgc gtccgtctga
//
LOCUS       DAOPNP010000021        24801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2547_length_24801_cov_9.197850, whole genome shotgun sequence.
ACCESSION   DAOPNP010000021 DAOPNP010000000
VERSION     DAOPNP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24801
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_24801_cov_9.197850"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKI64_03265"
     CDS             <1..240
                     /locus_tag="PKI64_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00063.1"
                     /translation="FLMVFIPSLGSYVIPDIVGGPTSEMIGNKIAQRTFVDRNLPHAA
                     GLSALLTLAVLIPMITITTLRRKESPKDALREEGV"
     gene            245..1030
                     /locus_tag="PKI64_03270"
     CDS             245..1030
                     /locus_tag="PKI64_03270"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00064.1"
                     /translation="MKPSRVPMIVTWAVILFFYLPIVILVANSFNSSRFGGSWESTSL
                     KWYIRLMQEDDIWHALQNTLFIALSATLVSMVLGTSAAFALHRFAGSRLQKLHYSIIY
                     TPLVLPEILMGLSMLLFFVALKVQLGLVTIAIAHVTFCLSYVAMTVLARLQDFDFAIV
                     EAAQDLGAGWWTTVWRVLLPMLAPGILAGGLLAFTLSIDDFVISFFVAGPGSTTLPIK
                     IYSMIKYGAPPLINALSTILLVITFTCVIVSQYLTQQEPKAAK"
     gene            1054..2094
                     /locus_tag="PKI64_03275"
     CDS             1054..2094
                     /locus_tag="PKI64_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE00065.1"
                     /translation="MILKKMLLAAGCAALLAALCGCADKKPVLHVYTWSDYVKPSLVK
                     EFERTHGCKVVVDTYDSNEAMYAKLKAGAKGYDLLFPSSYMVKIMNEQGMLQKLNHDL
                     LPNLGNVDPKYLHMAFDKEMDHSVPYTLTVTCLGYRASRVPDFEPSWNMLDRTEYKGR
                     MTMLNDQREAIGAALKFLGYSLNTTNDDELAAAKDVLVRWKKNIAKFDNEQYKNGLAS
                     GEFLITHGYSGDLMLVQTEEEDIAIAVPREGSVLSFDDMVIPADATQVELAHAFINFI
                     LEPRVAAELTEFIYFLCPNVPSYELLSEEVREDPILFPPDEVVAKLEMIADLGADNIK
                     YTHLWDAIKAAE"
     gene            2221..3720
                     /gene="glgA"
                     /locus_tag="PKI64_03280"
     CDS             2221..3720
                     /gene="glgA"
                     /locus_tag="PKI64_03280"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOE00066.1"
                     /translation="MDINSVAAGSPPLKVLFVASECAPFAKSGGLGDVVGSLPKALRR
                     LGADVRVALPLYDSIAREKYRLVEEPSCRVVCGGGEVNGCGVWRGMNDGEVPVWFIEH
                     NRYYARPGIYDDHGAEYGDNAFRFGLLCMAAAQVCHDRHWLPDVVHVHDWPTAVMAVL
                     LDAWRKAGVPLGRAAGVLTIHNQGYQGYYHPSALGYLGLGSEYFTPDGLEAWGKINLL
                     KGGINYADAITTVSPTYAREILGEPGGNGLSGYLQRRGDDFVGILNGADYDVWNPETD
                     RLIPANFRATSLIGKGQCKRELQAQMGLAQDAAVPLFGMVTRLAAQKGTGLLREALPA
                     ALNEMQLQLVVLGSGDHDDENFFRWLARSYPGRVAVEIGFSEELSHRIQAGSDFFLMP
                     SLFEPCGLSQLYALRYGTLPVVNATGGLEDTIIQYNQGEGQGTGFKFYTPEANALRAT
                     IGWAVSTWYDRPSHVTLMREQAMQQRFEWEASARKYLDVYRRAVAKRGG"
     gene            3724..4302
                     /locus_tag="PKI64_03285"
     CDS             3724..4302
                     /locus_tag="PKI64_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE00067.1"
                     /translation="MVEQTVGIVLAAGASQRMGMPKALLRGADGIPLAVRQTAVLAAG
                     GCAPVAIVLGAESERIRQELPATLRVVENTRWAQGRATSVQAGIVAFPEATGFLFMPV
                     DAVGVKPETVRAILAAAAREPAAIWRPVYQAAKGNLLWVSAAAAHELLNLPPDARVDD
                     WARPQARTLAVDDPAILRNINTPAEWAAFAAQ"
     gene            complement(4347..5363)
                     /locus_tag="PKI64_03290"
     CDS             complement(4347..5363)
                     /locus_tag="PKI64_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00068.1"
                     /translation="MIRWLPALLCLLALAVTAVAQDDLDALLAGLGDNEVVAAPVAAE
                     PAAAPEEAAVADPFADLLNAAEPVVEEASAAVEEVIETAPDVADPFATAPAADDPFAD
                     LLAAPAEEPVTEVVEDVVAEPIAEEAAAVEDPFADLLGEAPLAEEVPVAEEVVEDVIE
                     APVAEEAIAEEDPFADLLSEEPVVEESPIVEEVVEDVIAEPAVEEAVADEDPFADLLG
                     DEPVVEAAPVAEEVVEEAVVEEVESPDAPATQQDVDEAISMLKGKPKADKKAEPKKAK
                     DKKAEEPKKAKADDKKADEPKAKEKKADEPKAKPVEEKEAAKPVLKPRVADKAPDWNE
                     PIYW"
     gene            complement(5511..6362)
                     /locus_tag="PKI64_03295"
     CDS             complement(5511..6362)
                     /locus_tag="PKI64_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOE00069.1"
                     /translation="MKNIEKIAFQFPTTLAAALRLQANEKTRGKLLAGGTDLMVQWAS
                     GVPTPERVTSLWNLPEISAIRVLADHVEIGAGVTHAFLVDCQPLRTHVPALVAAAGTV
                     GAKQIQARGTLGGNVANASPAGDTAPALLVTDGTVVLASQAGEREVSLSQFWTGYRQI
                     AARPDEIIVAFRLPKRGKARERFRKIGTRRAQAISKVMAASRIRVEKGVIVSAAIALG
                     SVAPTPVRLTQVEQFLAGKKLTPALIAEAEKLAQTTVQPIADIRSTAEYRRWASGRLV
                     RDALEHL"
     gene            complement(6371..6856)
                     /locus_tag="PKI64_03300"
     CDS             complement(6371..6856)
                     /locus_tag="PKI64_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOE00070.1"
                     /translation="MSNVTRTWKLNGKSVTVRAPAGRRLLDVLRDDLGLTGSKESCGE
                     GECGACTVLVDDEPMVSCLIPVGQLPNGTEILTIEGIARKAAGRVIQQAYIECGAVQC
                     GFCIPGMVVSSYALLQRNPKPSTRAIRDALAGNLCRCTGYAKIIQAVKLAATRLAKRT
                     R"
     gene            complement(6853..9183)
                     /locus_tag="PKI64_03305"
     CDS             complement(6853..9183)
                     /locus_tag="PKI64_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOE00071.1"
                     /translation="MTKARKNARQLIGTGMHRVDAAGKVSGETRYLNDMPLKGILHGV
                     VVRSPVPRGILKAVEPDPAYNWTGITVATAKDIPGENVVHMHDRTMPLLAEVGAEVRY
                     RGEPVAVVAAKTLAQASAAAAHIRVEVDPLPPLLTLAEAVAVFKSTPDKFDSMKSQDI
                     VKGDIAKGFAEADDILEGEYEAGHQEQLYLENQGVVVYPQKDGSIFADGSLQCPYFPV
                     HELNDPLQLPPEKIRVRQTPIGGAFGGKEEYPTMLIGYAALPALKSGKPVKITYERNE
                     DILFTPKRHPVWVRYKTGFKKDGTLTAIECDYILDGGAYLTISDVVMFRGILHCALAY
                     RCDNVVIRGKVARTHSMPCGAFRGFGAPQAIWGIESHIDAVAAHLGRAPHEYRQQIHI
                     RPGDSTPTGQILSAENGSTTCLKQALQRADFARKWRQSSQGKLRKGQQKLYGVGLAFL
                     GHGSAFTGDGEAKFKAKGRVELATLANGKPGVNIRVSSTEMGQGVHTIFTQIAADALS
                     LAPEHIRFPLADTAFVPNSGPTVASRTTMVVGNVVYRAAQDLKQQIEAFAAQHFFRGA
                     AATLKDSQLVAKGARPVPFAKVAQAMLKEIGGASGSFQFNLPDSIRWDQTTFKGDSYP
                     GYSWMANIAEVEIDPLTYELAVTRVTAVIDIGRVINPVTAGGQVEGGLTQALGYAVME
                     KMGTGKNGLYDAARLQTYIIPTALDTPEFDIHFVEYPYTFAPPGAKGVGELPMDGLAP
                     AIANAVAAATGRRLRQIPITPEAIYEAVHGGREGTP"
     gene            9323..9877
                     /locus_tag="PKI64_03310"
     CDS             9323..9877
                     /locus_tag="PKI64_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOE00072.1"
                     /translation="MSMIQTNCDEGPRLPHPKIMLTGAIGAGKSTVVQAVLRELGWTT
                     PAGFRTHWGGQERGSAELFLEAWGGIPQVIARRDNTAAGFPYRLDHAAFISHARASLL
                     PCHQPVVLDELGVVELACDDFVTAIAQLFRAERPVLAVIQQRALDRWLERIGRDAVTE
                     LLTVDNATREHLPRHIAAVLCGSV"
     gene            complement(9886..11196)
                     /locus_tag="PKI64_03315"
     CDS             complement(9886..11196)
                     /locus_tag="PKI64_03315"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOE00073.1"
                     /translation="MVPQWIIEKKRDGHILSAEEIRFFINGFTQGSIPDYQMAAMAMA
                     IYLKGMTPEETAILTDSMMRSGAMVDTSSIKLPLVDKHSTGGIGDKVSLILAPLAACA
                     GLAVPMISGRGLGITGGTLDKLESIPGFNVNLTEKQFIETIHKCGFSMIGQTGEIAPA
                     DKKLYALRDVTGTVASIPLITASIMCKKMAEGIDSLVLDVKWGTGAFMKTIEDATTLA
                     KSMVRVGKAMNKGMVALLTDMNQPLGRTAGNALEIIESMECLQGQHAGEDLMNITLAL
                     TAEMLVLGKKAENTVAALEILRGHIVSGAAFAKFKEMAVLHGADVSTLDDLSKLPKAR
                     IIKPYLAPSAGYLAKVDAESIGRAVLVLGGGRQKTDDRIDYGVGTSGLAKIGDKVEKG
                     QPLVTVHANSEEKLATAMGYIEQAFGLTDTPVAPPKPIVDRITG"
     gene            complement(11335..11946)
                     /locus_tag="PKI64_03320"
     CDS             complement(11335..11946)
                     /locus_tag="PKI64_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOE00074.1"
                     /translation="MKKAILMIALVLLATTSMAQVIGLPIATTASSQPLGTVAASGGI
                     VLGDDLNLYGGRISFSPVDILAIFGDLGLIDPDEGKTGFAAQGGGLLTLPIRDLPVDV
                     GLRATAAYAAFDIPGGDLTMNTLNAGVLVSRDLNHISPYVFLGLNFADVTAKSHGYKA
                     TEDETDLALAAGLIVKLTTDFALYGEMAHVDNLFVAVGARWIH"
     gene            12190..13380
                     /locus_tag="PKI64_03325"
     CDS             12190..13380
                     /locus_tag="PKI64_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE00075.1"
                     /translation="MALVWASQFLSIMGFSFALPFAPYFLQQELGVITHAALQMWVAL
                     FSSSAAVTMAIAAPLWGVLADRYGKRIMLIRANLAGAVVMSLMGTVHTPLMLIVLRLM
                     QGALTGTMTAAQAFLAGEMPRERRGLAVGGLSAAVFSGSMAGAFCGGFVAHWAGYRAA
                     FGLSGILLLLAGLLVLCCTRETVVPAARAGRLAGQQPAAPRRAPLPAMLYGVLGMIGA
                     IAFVRQFDLAFVPLLVQDIHGSLEGVSLWSGALNACGSVAGLLAGLATGWLADRVNPL
                     RVVIVAAMVAAVFSGWQMVVHSFVVLFPIRFGNVFFAGALEPALNAWLAKHVPESRQG
                     VIFGLASTTRSIGWTIGPLVAGSVALVSLRGVFGFSALGFLVLALLFAVGLRRRDETA
                     TKAP"
     gene            13377..14117
                     /locus_tag="PKI64_03330"
     CDS             13377..14117
                     /locus_tag="PKI64_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009787547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE00076.1"
                     /translation="MSVTDPDCPFPETADIHTSADEYAARFAGPVGEWMLAVQERITR
                     RQLQVAAGGTVLDVGGGHGQLAVPLCRDGWRVTVLGSAESCRHRVREVVDSGACEFVV
                     GNVLALPFPDKSFDVVLCFRLLTHCERWPELVRELCRVARRAVIVDYPTGQSLNAIAP
                     ALFGAKQKYERNTRHWMLFRHRQIRDEFARHGYALTAQTKQFFLPMVLHRMLKSRALS
                     RGAEAVCRALGLTRLWGSPVIARFSPRD"
     gene            complement(14122..14904)
                     /gene="hypB"
                     /locus_tag="PKI64_03335"
     CDS             complement(14122..14904)
                     /gene="hypB"
                     /locus_tag="PKI64_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOE00077.1"
                     /translation="MCDTCGCGDPHLHPHDHAHDHAHDHTHHHEHGHGHSHDHVHDHA
                     HGHAHPPARTIEVQKPALALNQRYADQNRGWFRAKGLTVFNLLSSPGSGKTTLLEHTL
                     RAVPGCAAVVGDLQTENDANRLRATGAPAVQITTGATCHLDAHMVAHALPKLNLDGVT
                     RLFIENVGNLVCPASYDLGENRRVVLLSVTEGEDKPQKYPVIFHNADLVLITKCDLAE
                     AVGFDRDKALTIIRQVAPRAQILEVSARTGQGMAEWQAWLSS"
     gene            complement(14897..15238)
                     /locus_tag="PKI64_03340"
     CDS             complement(14897..15238)
                     /locus_tag="PKI64_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOE00078.1"
                     /translation="MHELSLMSQLLADATAAAEGAPIHALKVRVGPLSGVVPEALRFA
                     FESLIPGTPAANARLDLEETPLRFHCLRCHADYATPLGLYNCPQCGATDSELRGGNEL
                     ELISIEVPDHV"
     gene            15546..16025
                     /locus_tag="PKI64_03345"
     CDS             15546..16025
                     /locus_tag="PKI64_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00079.1"
                     /translation="MERRVTTEFDELLNAGAFVGSYTHSLDGKKRVTIPADWRDAAGN
                     PVLFVLPGVGFKCLYVVTAREMAQRMEKARASSVANVQAQKFMRDFFARADRVTLDAQ
                     GRIRVKDELLEYAGIVNQMVLIGTGIRFELWSPESWQEQSSQLDQSKFAEAAQYIGF"
     gene            16121..16531
                     /locus_tag="PKI64_03350"
     CDS             16121..16531
                     /locus_tag="PKI64_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00080.1"
                     /translation="MTLGRQAEMFDEAGQVTIAAKPAPAVKPAASRAIVSVQSRSRTT
                     ARVVATTRVQAATRVMAAPQAAARGEVARHLFMSKSLSSGKEGSRWFSVPQRVLLPRY
                     QLFEGADAVIEERPGQWRIGSPSLRQQRGQVWFV"
     gene            16550..17479
                     /gene="rsmH"
                     /locus_tag="PKI64_03355"
     CDS             16550..17479
                     /gene="rsmH"
                     /locus_tag="PKI64_03355"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOE00081.1"
                     /translation="MLHMPVLLAETVAHVVTEPGGWYVDATVGAGGHATALLAAAGLD
                     ARLLALDRDAEALELARAALAPFGARVVLEQANFADLAPVAARHGIRDVRGIVMDLGV
                     SSMQLDRPERGFSFLRDAPLDMRMDARQELTAAQLLAQAPEVELADILRRYGEETMAR
                     RIARALVQAREEGRLPATTTQLAELVAQAKGGRRGRTHPATQVFQALRIAVNGELSAL
                     ARALPAALDLLAPGGRLAVISFHSLEDRMVKQFMVAHEGRMEALAKGGARWQGQLPRA
                     RRVVRKAVAAAAEEVARNPRARSAKLRVLERMA"
     gene            17481..17837
                     /locus_tag="PKI64_03360"
     CDS             17481..17837
                     /locus_tag="PKI64_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00082.1"
                     /translation="MARRNRKNEQRGNRFAVWGIGIITILAVMMGLYLRLCSTCEQLG
                     RKIKEQEYLRAEMHKQVVNEQRNWSMACSIINMERMLAAQGIAMTFPEQKNIIRLKPL
                     EPADPLQYAQRNSARD"
     gene            17830..19569
                     /locus_tag="PKI64_03365"
     CDS             17830..19569
                     /locus_tag="PKI64_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE00083.1"
                     /translation="MIDRQYIWRTTLVVVLLLAAWSGLAVRLGMLHLNDNVKLRDRIQ
                     RMRIVKEELRVGRGRVLDRNENILALDLPVKDIRVDPVVVLSNGQARVISQVLARTLD
                     LPYKQVYDRVNRPGRRDEPIMRFATEEVADRVARLKMPGVFFRSQTMRFYPHTTLACH
                     VLGFSNMEGVGSAGIEQRLDSYLKGRSGFRESLRDGKRHELPDRRKIVIAAEEGADVH
                     LTLDMNVQYYLEKALDAAMTNFNPEAAWAIVQDVRTGFILGMASRPAYDLNIFNETGP
                     DIRLNRAIGVNYEPGSVFKIGVVAAAINEGLIATNDLFDCENGMWFYAGRPLKDFHPY
                     GILDVTGILRKSSNIGAAKIAVMLGPERLYRYLKEYGLGRTTGIELPGEETGILNPVG
                     KWAKVDITRIAMGHTVAVTALQMLNMICCIGNDGFLMKNHIVRKVVDKDGVVLFENKP
                     EAVARPITERTAALMRQMLMEVTREGGTGTRAAIPGFNVAGKTGTAEKIQDGRYVHNA
                     NVSSFMGIFPAERPEIGIVVVLDNPQPLRTGGVTAGPVFAQIAEPVARYLDIQPVNAT
                     EMGYYQRMLEDEP"
     gene            19566..21080
                     /locus_tag="PKI64_03370"
     CDS             19566..21080
                     /locus_tag="PKI64_03370"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOE00084.1"
                     /translation="MNRPGNGKAARPVKLNRLLAASPEAKLQAGAASTEITGLAYDSR
                     AVQPGFLFLALRGEKTNGALFAEDAIRRGAVAVLHDQDLRLPRGVAAIQVPDARLALA
                     DLAAEFYAHPSRQLRVLGVTGTNGKTTTTFMIRDILQAAGQSCGLLGTVQYEYGGRRL
                     AAGRTTPEALDLQRMLAEALHAGCQAVAMEVSSQGLAAERLRGVRFAAAVFTNLSVDH
                     LDFHHTMEAYFLAKKRLFDTIAAQGGAPAVINADDPHGRQLAADPALAGHVVTFGGDP
                     AAQVRAVDIQLTETSSTFRVITPWGEAAIATGFAGRFNVYNALAAIATCGTLGVPLPV
                     MAATLAAMPAVPGRMERIADPHGRHIFVDYAHTEDALRNVLQTLRENAPGHLLCVFGC
                     GGNRDRGKRPRMGAAVAEYADLAIVTSDNPRHEEPQAIIDDILAGMDPAKPHLVEPDR
                     AAAIATALRAARAGDTILIAGKGHEPYQEIAGRMAHFDDRETVRALLATIPPAT"
     gene            complement(21098..21976)
                     /locus_tag="PKI64_03375"
     CDS             complement(21098..21976)
                     /locus_tag="PKI64_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00085.1"
                     /translation="MRRICGWIVMALVVLGPLVGVAAPEAEALGLVRRFVEEELHGDF
                     AALATYDLSTLEGHALFGAPGRTFDTDDCDLVRAIFAVLYADALPGLNLETLGTGRAY
                     RGDTLNSFNTLFGRPIPDQPGRFAGLERYAPTDDLRARAAEFHHTYHTLGNLAPLPNL
                     SLERMTFNTYRGTHPGWRDAFPIFLQNLRLALLDDPAADPTLCRLVARNAAAFSEFRG
                     PDGLAEFAQRLDLDDYLDAATGLPLPLYSPNAHFATQSREDYLAAAEHYLTVATELIQ
                     RRAARMMARLQELLAE"
     gene            22065..22283
                     /locus_tag="PKI64_03380"
     CDS             22065..22283
                     /locus_tag="PKI64_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00086.1"
                     /translation="MDAEARRRVIAKFLSLTEEQQQKLCDAMGDSLAKLKADWEAKPE
                     NKGKAVTYTELWNEHTTHPPHNPDARGE"
     gene            complement(22522..24360)
                     /locus_tag="PKI64_03385"
     CDS             complement(22522..24360)
                     /locus_tag="PKI64_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOE00087.1"
                     /translation="MNEIHGLAKNIRALLSGCKYEIDYYQREYKWQTKHVAELLNDLT
                     DKFLDSYDSKHDRAKVEGYGHYFLGSIIISDKNGRKFIIDGQQRLTSLTLLLIYILRN
                     LHDAEQKSQLAPLIFSGKYGTKSFNLNVPEREVCMNAIFNGQIPHENGQPESIANIIG
                     RYRDIQEQFSKEVDAEAMPYFADWLIENVHLVEITAHTDEDAYTIFETMNDRGLSLTP
                     TDMLKGYLLANITNDNQRNQAGDTWKKRIAELNALGKEEDADAIKSWLRSQYAETIRE
                     RKRGASPQDFDLIGTEFHRWVRDNETRLGLEGSNTFAKFIDRDFSFYTGHYLMIRNAA
                     ETLTPGLECIHYNHQNDFTLQYPLLLAPLRIGDSNKDIQRKLRIVAAYIDILITRRIW
                     NFKAIEYSTMQYAMFLVMREIRAKSASELANLLAKRLEDKTSAPPFSNTDNFRLHGMN
                     GRQIHRILARMTDYVETQSGLASRYSEYIQRSGKKGYEVEHIWADHPELHKDEFDHPN
                     DFDEYRNRIGGLLLLPKTFNASYRDMPYKKKRTHYLKQNLLAQSLHENAYERSPRFKR
                     FVNRSGLNFQPMPVFKKAEMDARQQLYLRLAEQIWDPDRLFEEADA"
     gene            complement(24357..24569)
                     /locus_tag="PKI64_03390"
     CDS             complement(24357..24569)
                     /locus_tag="PKI64_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00088.1"
                     /translation="MNQITHNKMVSFIWSIADDVLRDLFKRSKFTRHFYKPQPLRILD
                     EIRADILAVQKESEGVLDGLLKEKTP"
BASE COUNT         4532 a         7417 c         7890 g         4962 t
ORIGIN      
        1 tttctgatgg tgttcattcc gtcgctgggg tcgtacgtca ttccggatat cgtcggcggt
       61 cccaccagcg aaatgatcgg caacaagatt gcccagcgca cgtttgtgga ccgcaatctg
      121 ccgcacgcgg caggactctc cgcactgctg acgctggcgg tgctgatccc catgatcacc
      181 atcaccacgc tgcgccggaa agaaagcccc aaagacgcct tgcgcgagga gggggtctaa
      241 ggccatgaaa cccagccgcg ttcccatgat cgtcacctgg gcggtgatct tgttctttta
      301 tctgcccatt gtgattctgg tggccaactc gttcaattcg tcgcgcttcg gcggttcgtg
      361 ggaatccacg tcgctcaagt ggtacatccg gctcatgcag gaggatgata tttggcacgc
      421 gctgcagaat acgctgttta ttgcgttgag cgccacactg gtttcgatgg tgctggggac
      481 ctccgcagcg tttgcgctgc accggtttgc cggcagccgc ctgcaaaagc tgcactacag
      541 catcatctat accccgctgg tgctgccgga aattctcatg ggcttgagca tgctgctgtt
      601 tttcgtggcg ctgaaggtgc aactggggct ggttaccatt gccatcgcgc acgtgacgtt
      661 ctgcttgtcc tatgtggcca tgaccgtgct ggcccgcttg caggattttg attttgcgat
      721 tgtggaggcc gcgcaggacc tcggcgccgg ctggtggacc acggtgtggc gcgtgctgct
      781 gccgatgctg gcgcccggca tcctcgccgg cggtctgttg gcgttcacgc tgtccattga
      841 tgactttgtc atttcgttct ttgtggcggg gccgggctcg accacgctgc ccatcaagat
      901 ttacagcatg atcaagtatg gcgcgccccc gctgatcaac gcgctgtcca ccatcctgct
      961 ggtaatcacg ttcacgtgcg tgattgtcag ccagtatctg acccaacaag agcccaaagc
     1021 ggctaaataa ccacaacccc aaggagaccc cccatgatac tgaaaaaaat gctgctcgcg
     1081 gccggctgtg ccgcgctcct cgccgcgctg tgcggttgtg ccgacaagaa gccggtgctg
     1141 cacgtctaca cgtggtcgga ttacgtcaag ccgtcgctcg tcaaagagtt tgaacggaca
     1201 catggctgca aggtggtcgt ggacacctac gactccaacg aggccatgta cgccaagctg
     1261 aaagccggcg ccaagggcta tgacctgctg ttccccagca gctacatggt caaaatcatg
     1321 aacgagcagg gcatgctgca gaaactcaat cacgatctgc tgcccaattt gggcaacgtg
     1381 gatccgaaat atctgcatat ggcgtttgac aaggagatgg accacagcgt gccgtacacc
     1441 ctcaccgtca cgtgcctagg ctaccgcgcg agccgtgtgc cggactttga accgtcgtgg
     1501 aacatgctcg accgcacgga gtacaagggc cgcatgacca tgctcaacga ccagcgcgaa
     1561 gccattggcg ccgcgctcaa gttccttggc tacagcctca acaccacgaa cgatgacgag
     1621 ctggccgccg ccaaagacgt gctcgtgcgc tggaagaaga acatcgccaa gtttgacaat
     1681 gaacagtaca aaaatggcct cgcgtccggg gaattcctca tcacgcacgg gtatagcggc
     1741 gatctcatgc tggtgcagac cgaggaagaa gatatcgcca tcgccgtgcc ccgcgaaggc
     1801 agcgtgctga gttttgatga catggtgatt cccgccgatg ccacacaggt ggaactggcc
     1861 catgcgttca tcaatttcat tcttgagccg cgcgtggctg ccgagctgac cgagttcatc
     1921 tacttcctgt gccccaacgt gcccagctat gaactcctca gcgaagaagt gcgcgaagac
     1981 cccatcctgt tcccgccgga cgaggtggtg gccaagctgg aaatgattgc cgaccttggc
     2041 gcggacaata tcaaatacac ccacctgtgg gacgccatca aggccgccga ataacccgtt
     2101 acggccctga ccggccccgc gtttgctgaa cgcggggcct ttttactgcc cggtggttgc
     2161 cacgaacttg gcttgtcttt gccattgtgc ggccgctggc cacgggccta tactgcgcgc
     2221 atggacatca attcagttgc cgccggctca cccccgctga aggttttgtt tgttgcttcg
     2281 gaatgcgcgc cgtttgccaa aagcggcggg ctgggcgatg tggttggcag cttgcccaag
     2341 gcgctgcgcc ggctgggcgc ggatgtacgt gtggcgctgc cactctatga ctccattgcg
     2401 cgcgagaagt accggctggt ggaagaaccc tcctgtcggg tggtctgcgg cggcggcgag
     2461 gtgaacggtt gcggcgtctg gcgcggcatg aacgatggcg aggtgccggt gtggttcatc
     2521 gagcacaacc gctactacgc gcggcccgga atctatgacg accacggcgc agaatatggc
     2581 gacaacgcgt ttcgcttcgg cctgctgtgc atggcggcgg cgcaggtgtg ccatgatcgc
     2641 cattggttgc cggatgttgt gcatgtacat gattggccca cggcagtcat ggccgtgctg
     2701 ttggatgcgt ggcgcaaggc cggtgtcccc ctcgggcggg cggccggcgt gctgaccatt
     2761 cataatcaag gatatcaggg ctattatcat ccatcggcgc tgggctactt gggcctgggc
     2821 agcgagtact tcacgccgga tggcctggag gcatggggca aaatcaatct gctaaaaggc
     2881 ggcatcaatt atgccgatgc catcaccacg gtctcgccca cctatgcgcg ggaaattctt
     2941 ggcgagccgg gcggcaacgg tctctccggc tatttgcaac ggcgtggcga tgactttgtc
     3001 gggatcctca acggcgctga ttatgatgtg tggaatccgg agaccgaccg gctgatcccc
     3061 gcaaacttcc gcgcgacatc gttgatcggc aaggggcagt gcaagcgcga gctgcaagcc
     3121 cagatgggct tggcgcagga tgccgccgtg ccgctctttg gcatggtgac acggctggcc
     3181 gcccagaaag gcaccggctt gctgcgcgag gctttgcctg ccgcactcaa cgaaatgcag
     3241 ttgcaactgg tggtgctcgg ctccggcgat cacgatgacg aaaacttctt ccgttggctt
     3301 gcccgttcat atcccggacg cgtggccgtg gaaattgggt tttccgagga gttgtcgcac
     3361 cgcattcagg ccggaagcga tttctttttg atgccgtcgc tgtttgaacc ctgcggcctg
     3421 agccagttgt atgcgctgcg ctatggcacg ttgcccgtgg tgaatgccac cggcgggctg
     3481 gaggacacca tcatccagta caaccagggc gaagggcagg ggaccggttt caagttttac
     3541 acgccggaag ccaatgcgct gcgggcaacc attggctggg cggtcagcac gtggtatgac
     3601 cggccgagcc acgtcacgct tatgcgtgag caggccatgc agcagcgttt tgagtgggag
     3661 gcctccgccc ggaaatatct ggatgtgtac cggcgcgcgg tggccaagcg cggaggctga
     3721 agcgtggtgg aacaaaccgt gggcattgtc ctggcggcgg gggcgtcgca acgcatgggg
     3781 atgcccaaag cgctgctgcg cggggccgac ggcataccgt tggccgttcg ccaaaccgca
     3841 gtgctggccg ccggcggctg cgctccagtg gccattgtgc tgggcgctga atccgaacgc
     3901 atccgccaag agttgccggc aacattgcgt gtggtagaaa atacccgttg ggcacaaggg
     3961 cgggccactt ccgtgcaagc cgggatcgtc gcgttccccg aggccacggg ctttcttttc
     4021 atgccggtgg atgcggttgg cgtgaagcct gaaaccgtgc gggccattct ggccgcggca
     4081 gcgcgggaac cggccgccat ttggcgaccg gtctatcaag cagcgaaagg caacctgcta
     4141 tgggtgtccg ccgccgcggc gcacgagttg ctgaacctgc cgccggatgc ccgcgtggac
     4201 gactgggcgc gcccgcaggc ccgaacgctg gcggtggatg atcccgccat tttgcgcaac
     4261 atcaataccc ccgccgagtg ggcggcattc gccgcgcaat aaaaaagccg gcggaaaatt
     4321 ccgccggctt gcaggccgca attgccttac caataaatcg gctcgttcca gtcgggagcc
     4381 ttgtcggcca cgcgcggctt caagaccggc ttggccgctt ctttttcctc aaccggcttc
     4441 gctttgggct catcggcttt cttttccttg gccttgggct cgtcggcttt cttgtcatcg
     4501 gccttggctt tcttgggctc ttccgccttc ttgtccttgg ccttcttggg ctcagccttc
     4561 ttgtcggcct tcggcttgcc cttcagcatg gaaatggcct catccacatc ctgctgagtg
     4621 gcgggggcat caggcgactc gacttcttcg acaaccgctt cttccacgac ttcttccgcc
     4681 acgggagctg cttcgaccac gggctcatcg ccaagcaggt cggcaaatgg atcttcatct
     4741 gcgacagcct cttccacggc cggctcagcg atcacgtctt cgaccacttc ttccactatc
     4801 ggcgattctt ccaccacggg ttcctcacta agcaggtcgg caaacgggtc ttcctccgca
     4861 atcgcctctt cggctacagg ggcttcgatc acgtcttcaa cgacttcttc ggccacgggg
     4921 acttcttcag ccaagggggc ctcgccgagc aggtcggcaa atgggtcttc cactgcggcg
     4981 gcttcttccg cgattggttc agcaaccacg tcttcgacga cttctgtcac cggttcctct
     5041 gcgggcgcag ccaacagatc ggcaaacggg tcatcggccg ccggcgcggt ggcaaacggg
     5101 tctgcaacgt cgggggcggt ttcaatcacc tcttccacgg cagccgaggc ttcttccact
     5161 accggttccg cggcgttcag cagatccgca aaaggatcgg caacagcggc ttcttcggga
     5221 gccgccgcgg gttctgcggc aacaggggcg gcaacgacct cgttatcgcc aaggccggcg
     5281 aggagggcat ccaaatcatc ttgggcaacg gccgtgacgg ccaaggccaa caagcacaac
     5341 agggcgggca accaacgtat catcgcatga ctccttgttt gagtggtcgc ggcgtaccat
     5401 acccgaatca ggcaggccgg tgcaatggat ttatgcgcag tttttcggcc aaacctgccg
     5461 gattgtatac cccgtaatcc gtgccgcgcg gcggaagcgc cgcctccagt ttagaggtgt
     5521 tcgagcgcgt cgcggaccag ccggccggag gcccagcggc ggtattccgc cgtggagcgg
     5581 atatccgcaa tgggctggac agtggtttgc gccagttttt cggcttcggc aatgagggcg
     5641 ggggtcagct ttttgccggc caggaattgt tccacctgtg tcaggcggac gggggtgggg
     5701 gccacggagc ccaacgcaat ggccgccgat acaatcacgc ctttttccac acgaatgcgc
     5761 gaggcggcca tgactttgct gatggcttgc gcgcgacggg tgccaatctt gcggaagcgc
     5821 tcgcgggctt tgccgcgctt gggcaggcgg aaggccacga tgatttcatc cggccgtgcg
     5881 gcaatctggc gatagccggt ccagaattgg ctcagcgaca cttcgcgttc gccggcttgc
     5941 gaagccagca cgacggtgcc gtctgtcacc agcagggcgg gggcggtatc gccggcgggc
     6001 gaagcattgg ccacgttgcc gccgagggtg ccacgtgctt ggatttgctt ggccccaacc
     6061 gtcccggcgg ccgccaccaa cgcgggcacg tgcgtgcgta gcggttgaca atccaccaga
     6121 aaggcgtggg tcacgcccgc gccgatttcc acatggtccg ccaaaacccg gatggcggaa
     6181 atttccggca ggttccacag cgaagtcacg cgctcgggcg tgggcacacc ggaagcccat
     6241 tgcaccatca ggtccgtgcc gccggcgagc aacttgccgc gagttttctc attggcttgc
     6301 aggcgtagcg ccgccgccag cgtggtcgga aattgaaatg cgattttttc aatatttttc
     6361 atggcgggcc tcaacgggtg cgtttggcga ggcgagtggc ggccagtttc acggcctgaa
     6421 taattttggc gtagcccgtg caacggcaga ggttgccggc cagcgcgtcg cggatggcgc
     6481 gggtggaggg cttgggattg cgctggagca gcgcatagga actgaccacc atgccgggaa
     6541 tgcaaaaccc gcattgcacc gcgccgcatt cgatataggc ttgctggatc acccggcccg
     6601 cagctttgcg cgcgatgcct tcgatggtca aaatttccgt accgttgggc agttggccca
     6661 caggaatcag gcaagagacc atgggctcat cgtccaccag caccgtgcac gcgccgcatt
     6721 ctccttcgcc acagctttcc ttggagccgg tcaggccaag atcatcgcgc agcacatcca
     6781 gcagtcggcg cccggcgggc gcgcggactg tgacggattt gccgttcagt ttccaagtgc
     6841 gggtgacgtt gctcatgggg tgccttcccg gccgccgtgg acggcctcat aaatggcttc
     6901 aggggtgatg gggatttgcc gcaggcgacg cccggttgcc gcggcaaccg cgttggcgat
     6961 ggccggcgca aggccgtcca tgggcagttc gcccacgcct ttggcgcccg gcggcgcgaa
     7021 ggtgtagggg tattccacaa agtggatgtc gaactccggc gtgtcaagcg cggtggggat
     7081 gatgtaggtc tggagccgcg ctgcgtcgta gagtccattt ttgcccgtgc ccatcttttc
     7141 catgacggcg tagcccagcg cctgggtcag gccgccttcg acctgcccgc cggccgtcac
     7201 cggattgatg acgcggccaa tatcaatgac cgccgtcacg cgcgtgacgg ccaactcgta
     7261 ggtcagcgga tcaatctcca cttcggcgat gttggccatc caggaatagc ccggatagga
     7321 gtcgcccttg aaggtggtct ggtcccagcg gatcgaatcg ggcaagttga actggaacga
     7381 gccgctcgcg ccgccgattt ccttcaacat cgcctgcgcc accttggcga atggcaccgg
     7441 tcgcgcaccc ttggcgacca gttgcgaatc tttcagcgtg gccgccgcgc cccggaagaa
     7501 atgttgggcg gcgaaggctt caatctgctg cttcaaatcc tgcgcggcgc gataaaccac
     7561 attgcccacc accatcgtcg tgcgcgaggc taccgtgggg cccgagttcg gcacgaaggc
     7621 ggtatccgcc agcggaaagc ggatatgttc cggtgccagc gaaagcgcgt ccgccgcaat
     7681 ctgcgtgaag atggtatgga cgccttggcc catctcggtg gaagacacgc ggatgttcac
     7741 cccgggcttg ccgttggcaa gcgtggccaa ctccacccgg cccttggcct tgaacttggc
     7801 ttcgccatcg ccggtgaacg ccgaaccgtg gccgagaaag gcgaggccaa cgccgtagag
     7861 tttctgctgc cccttgcgca gcttgccctg cgaggactgc cgccacttgc gcgcgaaatc
     7921 agcgcgttgc agcgcctgct tgaggcaggt ggtggagcca ttttccgcgg agagaatctg
     7981 gccggtgggg gtgctgtcgc cggggcgaat atggatttgc tggcgatact catgcggggc
     8041 gcggccgagg tgcgccgcca cggcatcaat gtgcgattca atgccccaga tggcttgcgg
     8101 cgcgccgaag ccgcggaacg cgccgcacgg catggaatgg gtgcgcgcca ccttgccgcg
     8161 gatcaccaca ttgtcgcagc gataggccag cgcgcaatgc aaaatgccgc ggaacatcac
     8221 cacgtcggaa atggtcaggt aggccccgcc atccagaatg taatcgcatt caatcgcggt
     8281 gagtgtgcca tcttttttga aaccggtttt atagcgcacc cataccggat ggcgcttggg
     8341 cgtgaagaga atgtcctcgt tgcgctcata agtgattttg accggcttgc cggatttcag
     8401 cgcgggcagc gccgcatagc cgatcaacat ggtgggatat tcctccttgc cgccgaacgc
     8461 gccgccgatg ggcgtttggc gcacgcggat tttttccggc gggagttgga gcgggtcatt
     8521 cagttcgtgc accgggaaat aggggcactg caacgagcca tctgcgaaga tggagccgtc
     8581 tttttgcgga taaaccacca cgccctggtt ttccaagtaa agctgttcct gatggccggc
     8641 ttcgtattcg ccttccagaa tgtcgtccgc ctcggcaaac cccttggcga tgtcgccctt
     8701 gacgatgtcc tggcttttca tcgagtcaaa cttgtcgggc gtggacttga aaacggccac
     8761 ggcttcggcc agcgtcaaga gcgggggcag ggggtccacc tccacgcgga tatgtgccgc
     8821 ggcggcgctg gcttgggcca gagttttggc ggccaccacg gccaccggtt caccgcggta
     8881 gcgtacttcc gcgccgactt ccgccagcag cggcatggtg cggtcgtgca tgtgcaccac
     8941 gttttcgccc gggatgtcct tggccgtcgc caccgtgatt ccggtccagt tgtaggccgg
     9001 atccggctcg accgccttga gaatgccgcg cggcacaggc gaacgcacca ccacgccatg
     9061 cagaatcccc ttgagcggca tgtcgttaag gtagcgggtc tccccgctaa ccttgccggc
     9121 cgcatccacg cggtgcatgc cggttccgat cagttggcgg gcattctttc gtgctttggt
     9181 catcagtcgc tcctgcgcgt gaatatccct aaaaacataa aatacctagc cccctgacgc
     9241 aaacaggaaa gcaggattca tcaaagcgtt ttatcggttg tggagttggc cgttggcgcg
     9301 caaccgggta tgctgctggc ccatgagcat gattcagaca aattgtgacg agggcccccg
     9361 actgcctcac cccaagatta tgctcaccgg cgccattggc gcgggcaaga gcaccgtggt
     9421 gcaagcggta ctgcgcgaat tgggctggac gacccctgcg ggctttcgca cgcattgggg
     9481 cgggcaagaa cgcggcagcg cggaattatt tctcgaagcg tggggcggaa tcccgcaagt
     9541 gattgcccgg cgtgacaaca cagccgcggg gttcccgtat cggttggatc acgcggcatt
     9601 catctcccac gcccgcgcca gcctgctgcc atgccaccag cctgtagtgc tggacgaact
     9661 cggcgtggtg gagttggcct gcgatgattt tgtcacggcc attgcgcaac tatttcgcgc
     9721 ggaacggcct gtgctggcgg tgattcaaca acgcgcgctg gatcgctggc tggagcggat
     9781 cggacgcgat gccgtcacag aactattgac ggtggacaac gccacccgcg agcatttgcc
     9841 ccgacacatt gccgcggtgc tgtgcggctc ggtgtaagcg cccgcctaac cggtgatgcg
     9901 gtccacgatc ggcttgggcg gcgcaacagg cgtatcggtg agaccaaacg cctgctcaat
     9961 gtagcccatg gcagtggcca gcttttcttc cgaattggcg tgcaccgtga ccagcggctg
    10021 gcccttctcc accttgtcgc cgatcttggc gaggccggaa gtgcccacgc cgtaatcaat
    10081 gcggtcgtca gtcttttggc gaccgccgcc cagcaccaat accgcgcggc cgatggactc
    10141 cgcatccacc ttggccagat agccggccga cggcgcgaga tacggcttga tgatgcgggc
    10201 cttgggcagc ttgctcaaat cgtcgagagt gctgacatcc gcgccatgca gcacggccat
    10261 ttccttgaac ttggcgaatg ccgccccgga cacaatgtgc ccgcgcagaa tctccagcgc
    10321 ggccacggta ttttccgctt tcttgcccag caccagcatt tccgcggtga gcgccagcgt
    10381 aatattcatc aggtcttcgc cggcgtgctg gccttgcagg cattccatgg attcaatgat
    10441 ctccagcgcg ttgccggccg tacggcccag cggctggttc atatccgtca gcaaagccac
    10501 catccccttg ttcatggcct tgcccacgcg caccatggac ttggccaggg tggtggcgtc
    10561 ctcgatggtc ttcatgaacg cgcccgtgcc ccacttgacg tccaacacca gcgagtcaat
    10621 cccttcggcc atctttttgc acatgatcga agcggtgatc agcggaatgg aagccaccgt
    10681 gccggtgacg tcccgcagcg cgtacaactt tttgtcggcg ggcgcaatct cacccgtttg
    10741 gccgatcatg gagaatccac acttgtggat ggtttcaatg aattgctttt ccgtcagatt
    10801 aacgttgaac cccgggatgg attccaactt gtcgagcgtg ccgccggtga tgcccagccc
    10861 gcggccggaa atcatcggca cggccaaccc cgcgcacgcg gccagcggcg ccagaatcag
    10921 tgagactttg tcgccgatgc ccccggtgga atgcttgtcc accagcggca gcttgatgga
    10981 agaagtgtct accatcgcgc ccgagcgcat catggagtcg gtcaggatcg ccgtctcttc
    11041 gggagtcatt cccttgagat aaatcgccat ggccatggct gccatctggt aatcgggaat
    11101 ggagccttgg gtgaatccat tgatgaaaaa ccggatttct tcggcgctca aaatgtgtcc
    11161 gtcgcgcttc ttttcaataa tccattgggg aaccatggca actccttatg acttgatttg
    11221 gccgcagcat ggccagcaac catttccggc gcaagccatt tttcggggaa accaagccgg
    11281 ccgcggcgga ttgcgcacgg ggccggtggg gtgttgacgc ggcgtggccg caggtcagtg
    11341 gatccagcgg gcgcccaccg ccacaaagag gttatccacg tgggccattt cgccgtagag
    11401 ggcaaagtcc gtggtgagct tcacaatgag accagccgcc agggccaagt ccgtctcatc
    11461 ttccgtggcc ttgtagccgt gggatttcgc agtcacatcc gcaaaattga ggcccaggaa
    11521 aacatagggg ctgatgtggt tcaaatcgcg gctgaccaga acacccgcat tgagagtgtt
    11581 catcgtgaga tcgccaccgg ggatatcaaa cgccgcgtag gccgccgttg cgcgcagccc
    11641 gacatccacg gggaggtcgc ggatcggcaa ggtcagcagg ccgccgcctt gcgcggcaaa
    11701 tccggttttg ccctcgtcgg gatcaatcag gccgaggtcg ccaaaaatcg ccagaatgtc
    11761 caccggcgaa aacgaaatgc gtccgccata taagttgaga tcatcgccca gcacaatacc
    11821 gccggaagcc gccaccgtgc ccagcggctg ggaactggcc gtggtcgcaa tcggcaggcc
    11881 gatcacttgc gccatgctgg ttgtggccaa caacaccagt gcaatcatca gaatcgcttt
    11941 tttcattttg tccctttcgc ttggtggagg ttctgctttc gctttttctt cgtgcaagct
    12001 ggcattctgt ttctttcacc ctcgaaaaga aagccttttg tgaccgccgc cggtcgcgtg
    12061 aaagcgacgc accggccacc gcgattgtgg gcttgcggcg ttgtcgtgcc gtccaaaaat
    12121 gctggctgga aagcggggtg ggggtggggc atagtcgtgg gcctcatgac ggattcgtgg
    12181 aaaaagcaaa tggccttggt gtgggcatcg caattcttgt ccatcatggg attttcgttt
    12241 gcgctgccgt ttgcgccgta ctttttgcag caggaactgg gggtgattac ccacgcggcc
    12301 ttgcagatgt gggtggcgtt gttttcgtcg tctgcggcgg tgacaatggc gattgccgcg
    12361 ccgttgtggg gggtgctggc ggatcgctac ggcaagcgca tcatgctgat tcgcgccaat
    12421 ctggcggggg cggtggtaat gtcgctaatg ggcacggtgc acacgccatt gatgctgatt
    12481 gtactgcggc tgatgcaggg cgcgctgacc ggcaccatga cggcggcgca ggcgttcctg
    12541 gccggcgaaa tgccgcgcga gcggcgcggc ctggcggtag gcggactgag cgcggcggtg
    12601 ttttcgggga gcatggcggg ggccttttgc ggcggttttg tggcgcattg ggccggttac
    12661 cgggccgcgt ttgggttatc gggaatcttg ctgttgctgg cgggtttgct ggtgctgtgc
    12721 tgcacgcgtg aaaccgtggt gcctgcggcg cgcgccggca gactggcagg gcaacaaccc
    12781 gctgcgccgc gccgcgcgcc gctgccggcc atgctctacg gtgtcttggg catgattggc
    12841 gcaatcgcgt ttgtgcggca gttcgatttg gcctttgtgc cactcctcgt gcaggacatc
    12901 cacggctcgc tggagggggt gtccctgtgg agcggggcat tgaatgcctg cggcagtgtg
    12961 gccgggctgc tggccggcct cgccaccggc tggctggccg accgggtaaa cccgctgcgg
    13021 gtggtcatcg tggccgccat ggtggcggcg gtgtttagcg gctggcaaat ggtggtgcat
    13081 tcattcgtgg tgttgtttcc gatccgcttc ggcaacgtct tttttgcggg cgcgttggag
    13141 ccggcgctaa acgcgtggct ggccaagcac gtgccggaat cgcggcaggg cgtgatcttc
    13201 ggtttggcca gcaccacgcg ctcgattggg tggaccatcg gcccgctggt ggcgggcagc
    13261 gtggcgctgg tgagcctgcg cggcgtgttt ggtttttcgg cactggggtt tctggtcctc
    13321 gcgctgctgt tcgcggtggg attgcgccgg cgcgacgaaa ccgctacaaa ggccccatga
    13381 gcgtgacgga tccagattgc ccgtttcccg agacggcgga cattcatacc tcggcggatg
    13441 aatacgcggc gcgttttgcc gggccggtgg gggagtggat gcttgcggtg caggagcgca
    13501 ttacccggcg gcaattgcag gtggcggcag gcggcacggt gttggatgtc ggcggcgggc
    13561 atgggcagtt ggcggtgccg ctgtgtcgtg atggctggcg cgtgacggtg ctggggagcg
    13621 cggaatcgtg tcggcaccgc gtgcgggagg tggtggattc cggcgcctgc gagtttgtgg
    13681 tgggcaatgt gctggcgttg ccatttccag ataagtcatt cgatgtggtg ctgtgcttcc
    13741 ggctgttgac gcattgcgaa cggtggcccg agctggtgcg cgagctatgc cgcgtggcac
    13801 gccgcgcggt gattgtggat taccccacgg gccagagctt gaacgccatc gcgccggcgc
    13861 tctttggcgc caagcaaaaa tatgaacgca acacgcgcca ttggatgctg ttccgccacc
    13921 gccagattcg ggatgaattc gcccggcacg gttatgccct caccgcgcaa accaagcagt
    13981 tcttcctgcc gatggtgctg caccggatgc tgaaaagccg cgcgctgtcg cgcggggcgg
    14041 aagcggtttg ccgtgcgttg gggctgacgc ggctgtgggg ctcgccggtg attgcgcgtt
    14101 tttcgccgcg cgactaaacc gttaggaaga taaccacgcc tgccactcgg ccatgccttg
    14161 gccggtacgg gcagacactt ccagaatctg cgcccgtggc gccacctgtc ggatgatggt
    14221 tagcgctttg tcgcgatcaa agcccacggc ctcggccaag tcgcacttgg tgatcagcac
    14281 gagatcggcg ttgtggaaaa tgaccggata tttttgcggc ttgtcctcgc cctcggtcac
    14341 ggagagcagg accacgcggc ggttttcgcc caagtcatag gatgccgggc aaaccaagtt
    14401 gccaacgttt tcgatgaata gccgggtcac gccgtcgagg ttcaacttgg gcagggcgtg
    14461 ggccaccatg tgggcatcga gatggcaggt ggcgccggtg gtgatctgca cggcgggcgc
    14521 gccggtggcg cgcaggcggt tggcgtcatt ctccgtttgc aagtcgccca ccacggccgc
    14581 gcagccgggc acggcgcgca gcgtgtgctc caagagcgtg gttttgccag agccggggga
    14641 ggagagcagg ttgaacacgg tgagcccctt ggcgcggaac cagccacggt tttggtcggc
    14701 atagcgctga ttcagggcca gcgcgggttt ttgcacttcg atggtgcggg ccggggggtg
    14761 tgcgtggcca tgggcatgat catggacgtg gtcatgggaa tggccgtgcc catgctcatg
    14821 gtggtgggtg tggtcatgcg catggtcgtg cgcgtggtcg tggggatgaa gatgggggtc
    14881 gccgcagccg caagtgtcac acatggtcag gtacctcgat ggagatgagt tccagttcgt
    14941 tgccgccacg cagctcgcta tcggtcgcgc cgcactgggg gcaattatac aggccaaggg
    15001 gtgtagcgta atccgcgtgg caacgcaagc aatggaagcg gaggggcgtt tcttccaaat
    15061 ccagccgcgc gttggcggcc ggggtgccgg ggatcagcga ttcaaacgca aagcgcaacg
    15121 cttccggcac cacgccggag agcggcccca cgcgcacctt cagcgcatgg atcggcgcgc
    15181 cttcggccgc cgccgtggca tcggccagca gttgggacat gagactcaat tcatgcatgg
    15241 ccgggcactc taccacaccg cgtcaaccgg cgctcgaaaa acctgcgaaa aatcccaatt
    15301 ttgagcccgc ctgcgcgcgc cattcacagc ccaaaatcgt gcgattctcc gcgaaaaatc
    15361 tggagattat gagcagcgaa aaaatcggct gtggataaca tgtggatatt ccacgaaaac
    15421 ccggcggcaa tcgtagttct tactgcttgc aggagagcaa gataaataaa gataaaaaat
    15481 atcacatttt tctgttgacg gttggtgggt caaagtggga taaagtgcat cgaaagaaca
    15541 gccaaatgga gcgtcgtgtc acaaccgaat ttgacgagct gctgaacgcc ggggcgttcg
    15601 tgggcagcta tacccactcg ctcgatggca aaaagcgcgt gaccatcccc gccgattggc
    15661 gtgacgccgc aggcaatccc gtgctgttcg ttctgccggg ggtgggcttc aagtgcctct
    15721 acgtggtgac cgcgcgcgag atggcccagc gcatggaaaa agcgcgtgca tcctccgtgg
    15781 ccaatgtgca ggcgcaaaag ttcatgcgcg actttttcgc gcgcgcggac cgcgtgacgc
    15841 tggacgccca gggccgcatc cgcgtcaaag atgaattgtt ggaatacgct ggaatcgtca
    15901 accagatggt gctgatcgga accggtatcc ggttcgaatt atggagtccg gaatcatggc
    15961 aggagcagag ttcgcaactg gatcaatcga agttcgccga agcagcccaa tacatcggct
    16021 tctaaacttc ctcggcgcct tcttcctcgg catcgggccg gctgccgcgc cgacgcgttc
    16081 caccgagaat tggtgggagc gcgaacccac cgatacgccc atgacgctgg gccggcaggc
    16141 ggagatgttc gacgaggccg ggcaggtgac cattgccgcc aagcccgcgc cggccgtcaa
    16201 gcctgccgcc agccgcgcga ttgtctcggt gcagtcacgt tcgcgcacca cggcgcgcgt
    16261 ggtggccacc acgcgcgtac aggccgccac ccgcgtcatg gctgcgccgc aagccgcggc
    16321 gcgcggcgaa gtggctcgcc acctgttcat gagcaaatca ctttccagcg gcaaggaggg
    16381 atcgcgttgg ttctccgttc cccaacgcgt cctcctgccg cgttatcagt tgttcgaagg
    16441 cgcggatgcg gtgatcgaag agcggccggg ccaatggcgc attgggtcgc ccagcctgcg
    16501 ccagcagcgc ggccaagtct ggtttgtcta acgcacaatg aggtgaggca tgctccatat
    16561 gccggttttg cttgcggaaa ccgttgccca cgtggtgacg gaacccggcg gctggtacgt
    16621 ggatgccacc gtgggggcag gggggcacgc cactgcgctc cttgccgccg ccgggctgga
    16681 tgcccgcctg ctggcgctgg atcgtgatgc cgaggctctg gaactggcgc gcgcggcgtt
    16741 ggcgccgttc ggtgcgcggg tggtgctgga gcaggccaac ttcgcggacc tcgcgccggt
    16801 ggccgcccgc cacggcatcc gcgatgtccg cggcatcgtg atggatttgg gtgtgtcctc
    16861 catgcagttg gaccggccgg aacgcggctt cagcttcctg cgcgacgccc cgctggatat
    16921 gcggatggat gcgcggcagg agttgaccgc cgcgcaattg ctggcccaag cgccggaagt
    16981 cgagctggcg gacatcctgc gacgctatgg cgaggaaacc atggcgcggc ggattgcgcg
    17041 cgcgctggtg caggcgcggg aggagggccg gctgccggcc accaccacgc aactggccga
    17101 gctggtggcg caggccaagg gtggccggcg cggccggacg catcccgcca cgcaggtgtt
    17161 ccaggcgctg cggatcgcgg tgaatggcga gttgtccgcg ctggcgcggg cgctgccggc
    17221 ggcgctggat ttgctggcgc ccggcggccg gctggcggtg attagttttc acagtttgga
    17281 agatcgaatg gtcaaacagt tcatggtggc tcacgaaggc cgcatggagg cgctcgccaa
    17341 gggcggcgcg cgctggcagg ggcaactgcc gcgcgcgcgc cgggtggtgc gcaaggccgt
    17401 ggcggccgcg gcggaggaag tggcgcgcaa cccgcgcgcg cggtcggcca aattgcgggt
    17461 gttggaaagg atggcgtgac atggcaaggc gtaatcgcaa aaacgagcaa cggggcaacc
    17521 ggttcgcggt gtggggcatt ggcatcatta cgatcctggc cgtcatgatg ggcctctacc
    17581 tgcggctgtg cagcacgtgc gagcaactgg ggcggaaaat caaggagcag gaatacctgc
    17641 gcgccgaaat gcacaagcaa gtggtgaacg agcagcgcaa ttggtccatg gcctgttcca
    17701 tcatcaacat ggagcgcatg ctggccgcgc agggcatcgc catgactttt cccgaacaga
    17761 aaaacatcat ccgcctgaag ccgttggagc cggcggatcc gttgcaatac gcccagcgca
    17821 acagcgcgcg tgattgaccg tcaatacatc tggcgaacga cgctcgtcgt agtgctgctg
    17881 ctggcggcct ggagcggcct ggccgtgcgg ctgggcatgt tgcacctcaa cgataacgtc
    17941 aagctgcgcg accgcattca acgaatgcgc atcgtgaagg aagaactgcg cgtgggtcgc
    18001 ggacgcgtgc tggaccgcaa cgagaacatc ctggcgctgg acttgccggt caaggatatc
    18061 cgtgtggacc ccgtcgtggt gctcagtaac gggcaggcgc gcgtcatcag ccaggtgctc
    18121 gcccgtacgc tggacctgcc ctataagcag gtttatgacc gcgtcaaccg ccccggccgc
    18181 cgcgatgagc ccattatgcg cttcgccacc gaggaagtcg ccgaccgtgt tgcccgcctc
    18241 aaaatgcccg gtgtgttttt ccggtcgcaa accatgcggt tttatccgca caccaccctg
    18301 gcctgccatg tgctgggttt ttccaacatg gaaggcgtgg gcagcgccgg catcgagcag
    18361 cggctggatt cctacctcaa agggcgcagc ggattccgcg aaagcttgcg cgacggcaaa
    18421 cgccacgagc tgcccgatcg gcgcaagatc gtcatcgccg ccgaggaggg ggccgatgtt
    18481 catctgacat tggacatgaa tgtccagtat tacctcgaaa aggcgctgga tgccgccatg
    18541 accaacttca accccgaggc cgcctgggcc atcgtgcaag atgtgcgcac cggcttcatt
    18601 ttgggcatgg cctcgcgccc ggcctacgac ctcaatatct tcaatgaaac cgggccggat
    18661 atccggctca accgcgccat tggcgtgaac tatgagccgg gctctgtgtt caaaattggc
    18721 gtggtggccg cggccatcaa tgaaggcctc attgccacca acgacctctt cgattgcgaa
    18781 aacggcatgt ggttctatgc cggccgcccg ctgaaggatt ttcaccccta cggcattttg
    18841 gatgtgaccg gcatcttgcg caaatccagc aatatcggcg cggccaaaat cgccgtgatg
    18901 ctcggtccgg agcggctgta ccgctacttg aaagagtacg gccttggccg caccaccggc
    18961 attgaattgc ccggcgagga aactggcatc ctcaaccccg tgggcaaatg ggccaaggtg
    19021 gacatcacgc gcatcgccat ggggcacacg gtggcggtca ccgcgctgca aatgctcaac
    19081 atgatttgct gcatcgggaa tgatggtttt ctgatgaaaa accacattgt gcgcaaagtg
    19141 gtggacaagg atggcgtggt gctgttcgag aacaagcccg aagccgtggc gcggcccatc
    19201 accgagcgca ccgcggcgct catgcggcaa atgttgatgg aagtcacccg cgagggcggc
    19261 accggcaccc gcgcggccat tcccggcttc aatgtcgccg gtaaaaccgg caccgccgaa
    19321 aaaatccagg acgggcgcta cgtccataat gccaatgtat cctcgttcat gggcattttc
    19381 cctgccgagc gcccggaaat cgggattgtc gtcgtgctgg ataatccgca gccgctgcgc
    19441 accggcggcg tgacggccgg cccggtcttc gcgcaaatcg ctgaacccgt cgcccgttac
    19501 ttggatatcc agcccgtcaa tgccaccgaa atgggctatt accagcgcat gctggaggat
    19561 gaaccttgaa ccgccccggt aatggcaagg ccgcgcgccc cgtcaaactg aaccgactgc
    19621 tggcggcgag tcccgaggcc aaattgcagg ccggtgccgc ctccacggaa atcaccgggc
    19681 tggcttacga ttcccgcgcg gtgcaaccgg gcttcttgtt cctggccctg cgcggcgaga
    19741 aaaccaatgg cgcattgttc gccgaagacg ccatccggcg cggcgccgtc gccgtgttgc
    19801 acgatcagga tttgcgcctg ccgcgtggag ttgccgccat ccaagtgccc gatgcccgcc
    19861 ttgcgctggc cgatctggct gcggagttct atgcccaccc ctcgcggcaa ctgcgtgtgc
    19921 tgggcgtcac cggcaccaac ggcaaaacca ccaccacctt tatgattcgc gatatcctgc
    19981 aagccgccgg gcaatcctgc gggctgctgg gcaccgtgca atatgagtat ggcgggcggc
    20041 gtctcgctgc cgggcgcacc acgccggaag ccctcgacct gcaacgaatg ctggccgaag
    20101 ccctgcatgc cggttgccag gccgtggcca tggaagtgtc gtcgcaaggc ctggccgcgg
    20161 aacgcctgcg cggagtccgc tttgccgctg ccgtgttcac caacttgagc gttgaccacc
    20221 tcgacttcca tcacaccatg gaggcctatt ttctcgccaa aaagcggctg ttcgacacca
    20281 tcgccgctca aggcggtgcg cccgccgtca tcaatgccga tgacccccac ggacggcaac
    20341 tggccgccga tcccgcgttg gccgggcacg tcgtcacgtt tggcggcgat ccggccgcgc
    20401 aggtccgcgc ggtggatatt cagttgacgg aaacgtcttc cacgtttcgc gttatcacgc
    20461 cgtggggcga ggcggccatt gcaaccggtt tcgccggccg cttcaatgtg tataatgcgc
    20521 tagcggccat tgccacctgc ggcacgttgg gcgtgccgct gcccgttatg gccgccaccc
    20581 tcgccgccat gccggcggtg cccggtcgca tggagcgcat cgccgatccc catggtcgcc
    20641 acattttcgt ggactatgcc cacaccgagg atgccctgcg caacgtgttg caaaccctgc
    20701 gcgaaaatgc gcccggccac ctgttgtgtg tctttggctg cgggggcaac cgcgaccgcg
    20761 gcaaacgccc gcgcatgggc gctgccgtgg cggaatatgc cgacctcgcc atcgtcacca
    20821 gcgacaaccc ccgccacgag gaaccgcaag ccatcattga cgatatcctc gccggcatgg
    20881 accccgccaa gccgcatctg gttgagcccg accgggccgc cgccatcgcc accgcactgc
    20941 gtgccgcgcg cgcgggcgac accattctca tcgccggcaa aggccacgag ccctatcagg
    21001 aaatcgccgg gcgcatggcg cacttcgatg accgcgaaac cgtgcgcgcc ctgctcgcaa
    21061 ccattccgcc ggccacctga tccctaccga ttgattttta ctccgccaac agttcctgca
    21121 accgggccat catgcgcgcg gcccggcgtt gaatcagctc cgtagccacg gtcagataat
    21181 gctccgccgc cgccaaataa tcctcgcggc tttgcgtagc aaaatgcgcg ttgggggaat
    21241 aaagcggcag cggcaggcct gtagcagcat ccaggtaatc atccaaatcc agccgctgcg
    21301 cgaattccgc caaaccatcc ggcccgcgga actcgctgaa tgcagccgca ttccgcgcca
    21361 ccaaccggca caatgtcggg tccgccgcgg ggtcgtccag caaggccagc cgcaagtttt
    21421 gcaaaaagat gggaaacgcg tcgcgccaac ccggatgggt tccgcggtac gtgttgaacg
    21481 tcattcgctc cagcgacaaa ttggggaggg gagccagatt gcccagcgtg tgataggtat
    21541 ggtgaaactc cgccgcccgc gcacgcagat catcggtggg ggcatagcgc tccaagccgg
    21601 caaaccggcc gggctgatcc ggaatgggcc ggccaaacag cgtgttgaaa ctgttgagtg
    21661 tgtcgccccg ataggcgcgc cctgtgccga gcgtttccaa attcaagccc ggcagcgcat
    21721 cggcgtacag caccgcgaaa attgcccgca ccagatcaca gtcatcggtg tcgaacgtgc
    21781 gccccggtgc gccgaagagc gcatgcccct ccaacgtgct caaatcgtac gtcgccagcg
    21841 ctgcgaagtc tccgtggagt tcctcctcca cgaaccggcg caccagcccc aacgcctccg
    21901 cctccggcgc ggcaaccccc accaacggcc ccaacaccac cagcgccatc acgatccagc
    21961 cacatatccg tctcatgccg cccacactac caaccttccc attcttccgg caatcattcg
    22021 gaaatcattg atcctgatag ggggatctgt ggtatggctt gggcatggac gcggaagcac
    22081 gccggcgggt gatcgcgaag tttctgtcgc tgaccgaaga gcagcagcag aaactgtgcg
    22141 acgcgatggg cgatagtctg gccaaactca aggccgattg ggaagccaag ccggaaaaca
    22201 agggcaaggc cgtcacctac accgagctgt ggaatgagca caccacacac ccgccgcaca
    22261 accctgacgc cagaggggaa tagtctcctc aacccaagca aggggcattc gcgcgtcaag
    22321 cgcaggcgat ctggtacacc atatcggtca caaagtggcg gaaggggttg ttctcgacaa
    22381 actgtttata gacctgagta tcgtcgcgca gaaggggcag catgactcag gctagggcct
    22441 tgtcgtgctc gatacgaggc accttgccaa gccacggcag ccctgagtct tagtatcagg
    22501 gaaagggttg gagtcggctg atcatgcgtc cgcctcttcg aacaggcgat ccgggtccca
    22561 gatttgctcg gctaggcgca gatagagttg ctgacgtgca tccatttcgg ctttcttgaa
    22621 aaccggcatt ggctggaagt tcagtccgct gcggtttacg aaccgcttga accgcgggct
    22681 gcgttcgtag gcattttcgt gcaggctctg ggccagcagg ttttgcttca ggtagtgcgt
    22741 ccgcttcttc ttgtagggca tgtcgcgata gctggcgttg aacgttttag gcagcagaag
    22801 cagccccccg atacggttgc ggtattcgtc gaagtcgttt ggatggtcaa attcatcctt
    22861 gtgcaactcg ggatgatccg cccagatgtg ctcaacctcg tagccttttt tcccgctgcg
    22921 ctggatatac tccgaatagc gcgatgccaa cccagattgg gtttccacgt agtcggtcat
    22981 gcgggccaga atgcgatgga tctgccgacc gttcatgcca tggaggcgga aattatccgt
    23041 gtttgagaag ggtggagctg aagttttatc ttcgagacgc ttggctagga ggttagccaa
    23101 ttccgaggcg cttttggccc ggatttccct catcacaagg aacatggcgt attgcatggt
    23161 ggagtactca atggccttga aattccagat gcgccgggtg atcagaatat cgatgtaggc
    23221 agcgacgata cgcagcttgc gctggatgtc cttgttggag tcgccgatac gcagcggggc
    23281 cagcagcaga gggtactgga gggtgaagtc gttctgatgg ttgtaatgga tgcactctag
    23341 ccccggggtc aacgtttcgg ccgcgttgcg aatcatcaaa taatgaccgg tgtagaacga
    23401 aaaatcacga tcaatgaact tggcgaaagt gttgctgcct tccagtccca gtcgcgtttc
    23461 gttgtcccgg acccaccggt ggaattctgt tccaatcaag tcaaagtctt gcggcgaagc
    23521 cccgcgcttg cgttcgcgga ttgtttcggc gtactggctg cggagccaag acttgatggc
    23581 gtcggcgtcc tcctccttgc caagagcatt gagttcagcg atgcgctttt tccaggtgtc
    23641 gccagcctgg ttgcgctggt tgtcgttggt gatgttggcc agaagatagc ccttgagcat
    23701 gtcggtgggg gtgagagata gcccgcggtc gttcatggtt tcaaagatgg tgtaggcgtc
    23761 ctcgtccgtg tgcgcggtga tttccacgag atgaacattc tcaatgagcc agtccgcgaa
    23821 atacggcatg gcctcggcat ccacttcctt tgagaactgc tcctgaatgt cacggtaacg
    23881 gccgatgatg ttggcgatgg attcaggctg gccgttttcg tggggtatct ggccgttgaa
    23941 aatggcattc atgcagactt cgcgctcagg cacgttgaga ttgaaggatt ttgtcccgta
    24001 tttcccggag aaaatgaggg gcgccagttg gcttttctgc tcggcatcat gcaggttgcg
    24061 gaggatgtag atcagcagga gggttaggga cgtgaggcgt tgttgaccgt caatgatgaa
    24121 cttgcggccg ttcttgtcgc tgatgatgat ggagccaaga aaatagtgcc catagccttc
    24181 caccttggct cgatcatgct tagagtcgta gctgtcgagg aacttgtcgg tcaggtcgtt
    24241 gagcagttcg gccacatgct tggtttgcca cttgtattcg cgctggtagt agtcgatctc
    24301 gtacttgcag ccactcaaca gggcgcggat gttcttggcg aggccgtgta tctcattcat
    24361 ggagtcttct cctttagcaa cccatccaga actccttccg attctttttg gacggcgagg
    24421 atgtcggcgc gaatttcgtc gagaatgcgg aggggctggg gtttgtaaaa atgacgggtg
    24481 aacttgctac gtttaaacag gtcgcggagc acgtcgtcgg caatgctcca gatgaaagaa
    24541 accattttat tgtgggtgat ttggttcatg gggttaccac gtcaaatgtt ggaacgggtt
    24601 gggacgcttg taaatcataa gcggacggta gcagaagagt taggcatggg gcaagaacgg
    24661 gaatgctgcc gcctgttgcc tgacaaggcc catttgcgca ctattcctcg aaggcacaaa
    24721 aaagccctga aaaccggtgt tttcagggga tcaagatggt agaggcggag gggctcgaac
    24781 ccacgaccca ctgattaaga g
//
LOCUS       DAOPNP010000022        24573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2574_length_24573_cov_9.073619, whole genome shotgun sequence.
ACCESSION   DAOPNP010000022 DAOPNP010000000
VERSION     DAOPNP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24573
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2574_length_24573_cov_9.073619"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKI64_03395"
     CDS             <1..285
                     /locus_tag="PKI64_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00089.1"
                     /translation="ALHETIRDTGCTLKAFRAEWTAELPMQFRGMHRFLPALMAMAGA
                     RIAEIPVNHRPRAAGQSKYTNWGRLKETLWDLWAVRWMQKRYRSRALASD"
     gene            414..668
                     /locus_tag="PKI64_03400"
     CDS             414..668
                     /locus_tag="PKI64_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOE00090.1"
                     /translation="MGLLGMAGQVLFFSRFLVQWIASEKKGRSTVPLSFWYLSIGGGL
                     LLLVYAIWRKDPVITIGQAVGLFVYIRNLMLIYQHRGRVG"
     gene            673..>1826
                     /locus_tag="PKI64_03405"
     CDS             673..>1826
                     /locus_tag="PKI64_03405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE00091.1"
                     /translation="MNTPRLWQLFLLALLLWGGETLRRDLWNPDEVRYAYIAREMAQD
                     QHWLVPFRSGEYYAHKPPYLFWAINLVSHATRMPIGRIPVRLPGLLAGVFVLWTTARL
                     ATRWAGARAAWPAVLTLCANYMFWHEIGFARMDGPLLGWTMAALTLLFLNDDQPTLWR
                     PAVAWLCMGMGVLTKGPVGLLTPAGAYIAARLAAGEGRLLKKSHWLWGPFLGLALPGL
                     WLALAWRHGAPPGYFSELLFSQNIQRAAGILGHRQSFAYFFGVAPAHFLPWTLCLPAA
                     ILVLRRTPEQRQLLRRLLGWIGFIFVFFSLMTSKRTIYILGLFPALAILVGGTWTELA
                     TATDRWARLARGAFLWLLTLAGPAVLIASFLPLPIPGRVLWPTGLAALAGA"
     assembly_gap    1827..1926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1927..2525)
                     /locus_tag="PKI64_03410"
     CDS             complement(<1927..2525)
                     /locus_tag="PKI64_03410"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOE00092.1"
                     /translation="MRTRYEKLRIPFSRGLAVVAVFFLLFSSSRWEETSPLITTMLFT
                     VGMLLVAVASLGRMWCSVYIAGYKDRTLITAGPYSMSRNPLYFFSMLGALGVGCCTEA
                     FTFPVLILLAMALYYPLVIRKEERRLQEYFGSAFSDYVQRVPVFFPKLSLFREPDTYT
                     VNPRAYRRHMFSALWFVWLVGLIELAEGLKEIGWLRSFW"
     gene            complement(2618..6190)
                     /gene="dnaE"
                     /locus_tag="PKI64_03415"
     CDS             complement(2618..6190)
                     /gene="dnaE"
                     /locus_tag="PKI64_03415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE00093.1"
                     /translation="MEPTFTHLHLHTEYSLLDGLCALDRGEKHRSPLMERAVEQKQVA
                     MAITDHGNLYGAIHFYKAARKHGIKPIMGCEAYVTPGNHREKVLVNGRQANHLVLLAE
                     NNRGFENLMKLVSRAHLDGYYYKPRIDRALLAEYSEGLIALSACLKGEVAEALVDGLD
                     DKAEQLAGEYAEIMGPGRFYLELQEHGLPEQGPVNRKLVALSRKLGLPIVATNDVHYL
                     SRDHVEPHEMLLCLQTQAKWSETDRMKYGSNQFYLKNEAEMRELFRELPEAIENTWKI
                     AERCNVTLALDGQKNPHFPNYQCPGGLSHKQYLTQIATEGVRRLYGVDDPEHPRDDRE
                     RTIVERMNHEIAVIEKMGFLNYYLVVWDFIHAAKQMGVPVGPGRGSGAGSLVAYAMGI
                     TGIDPQRYGLIFERFLNPERISPPDFDIDFCQTRRGEVLDYVRRKYGADSVVQIITYG
                     TLGAKTLIRDLGRALEFPLGECDRLAKMVPEVPGTTLEKALNTSVDFKTACRTEPYAI
                     EIMKYAPHLEDMPRQTGTHAAGVVIGEKPLFELVPLTRDKDGNVISQWEAGPLEDLGL
                     LKMDFLGLKTLTVVREACDNIRQTRGIEINPDDLPLDDAETYQLLARGDTVGVFQVES
                     DGMRDLLRQLQISKIEELIAMIALYRPGPMSTIPSYIARKHGRETISYAHPLLEDVLK
                     ETYGIMVYQEQVQQAAGLLAGFSMGQGDILRRAMGKKQAEQMRQQRQAFVDGCLKKGT
                     CNAAKAGEIFDQIAAFAEYGFNKSHSAAYSMVTFQTAYLKAHYPVEFMAALLSSEIGN
                     TDKLPVLVAEAQKMGIPVLPPDVNESGARFTPLQDKIRYGLAGVKGVGQGAVAALVKE
                     REANGPFLGFVDFCERMDSSEVNKKTLEALIKCGAFDFTQLMRGRLYGGIEAAVSYAA
                     TRRADRAAGQASLFEMAGADSSGAHMQDDDLPILPPWSQKDMLAYEKELIGFYISGHP
                     LLAHTWTLAKFGKCDANSFAALPESTPTRIGGLVINAQKRFTKPTKQRPTPQAMMSFQ
                     LDTLEGTVSAVAFPEAFEKYGGLLTPDAAVMLCGTVRKDNGSSANVLAVDEIHPLDMV
                     PEKFALSATVHIPVGIWEDAKMKALQELLRRHPGTTAVNLSLIFPDSLKVYIATGKTF
                     AVRVSEAFAKECEKLGLNLYVETRREACLQPPPAKRGWSRNRGG"
     gene            complement(6375..7211)
                     /locus_tag="PKI64_03420"
     CDS             complement(6375..7211)
                     /locus_tag="PKI64_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOE00094.1"
                     /translation="MIDLHCHSTFSDGSLTPEQLADEAVKIGLKALALTDHDTVAGLP
                     RFLAAAEGRSFRAVPGVEISVDCAFGVMHMLGYWMDTAHPELVRQMEWIRNGRHMRNT
                     TMLEKLNALGFAMTWDEVQAVAGEDVVGRPHFAQVMLAKGYAKDKNEVFDKWLGDGKP
                     GYADRPRLTAEAAVALIRQAGGVAVLAHPFTLRISRDDMLALWRQLAAAGLAGVECYY
                     SEHSADLTKDYLAMAREVDLVPTGGSDFHGEVSPGIRLGVGFGGLNVPDECLAQLEAR
                     RG"
     gene            complement(7290..7868)
                     /locus_tag="PKI64_03425"
     CDS             complement(7290..7868)
                     /locus_tag="PKI64_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00095.1"
                     /translation="MKTKQLSCCLVLALLAVSAWADPYHHEATGIIFPDVLAGLDKDA
                     EVVDYEARQAGLGISVRYGSPASKVDVYLYTMGRDSIPGDLHAPLVKEHFMQASEEII
                     GVAEQGLYENFKVLSEGELASKEAGVLPVWRAIYLFSRAGQDYRSYLYVATFHNHFLK
                     VRYTILAESADDHDRDADDFWAALCHLLPTDQ"
     gene            complement(7992..9689)
                     /gene="rpsA"
                     /locus_tag="PKI64_03430"
     CDS             complement(7992..9689)
                     /gene="rpsA"
                     /locus_tag="PKI64_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOE00096.1"
                     /translation="MMAMYAETFKTIAEGAIVKGHVVEVRPQEVLVNIGYKSEGLLAA
                     SEFKDLSEVHVGDEVEVYLERLEDEHGMVVLSKTRAEQQRNWDNVTASCEEGKVVTGL
                     VKGKVKGGLLVDVGGVDAFLPGSQIDVVPARNLDDYLGQSLECKVIKINKERRNIVLS
                     RRDLIEETRREQKKKLLQEIQPGQTRRGVVKNITEFGAFVDLNGMDGLLHITDMSWGR
                     INHPSEVVQVGDELEVVVLDVNLDKERISLGLKQKQQNPWEEIELKYPKGTRIRGKVV
                     NLVPYGAFVQLEDGVEGLVHVSEISWTKRVARAADALSVGQEVDAVVLNVNKEEQKIS
                     LGIRQTEVNPWDDAAAKYPIGSRVKGKVRNFTNYGAFVELEEGVDGMIHVSDMSWARK
                     VSHPNEVLKKGDEVEAIVLEVSPENQRISLGLKQAQEDPWAGISNRYRIGQLIKGKVS
                     KLASFGAFVELEEGVDGLVHISQISDDHVTKIRDVLQPGQEVEARVIKIDPIDRRIGL
                     SIKAAKLPEEDFKVDDSMLEGLRAGEDLVDLAGAFDAALSSSDKNPEEWSPGASQKE"
     gene            10315..12735
                     /locus_tag="PKI64_03435"
     CDS             10315..12735
                     /locus_tag="PKI64_03435"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOE00097.1"
                     /translation="MTWRQILVVALGCLLVSLVVFHVVLPPGSILMTTDDNIGHNTTA
                     KNILPMGFRAWWDAQALWGTSGISVFRPMYVLLYLLPVEVYQNWAHGLALAAASFWLV
                     VYLRRRGCSWAACVLGGLTAFWTGNNLTLTYAGHTNKFATMAFAAAAIALLDRAATDR
                     RWGWGALAGMAVGFMFFEQADVALFSAVFIAAFGLYRAVQAADGWKPWPMVRLLLPAA
                     VIGGVMVLGAVGPYQKVATEGITVMQQDPQVQYDFLTQWSWPPEESLDFIAPGFTGWR
                     SGEESGPYWGRMGRSAGWETTRQGFMNFKLENHYMGALPVLLALFAFGGAGAHWLRKK
                     RDARTADTLFWGLVCAGALVLAFGKFTPIYRLVAMLPGFSGMRNPNKFLHIFQMALGI
                     VAAFGWDGLLNGRLGERPGRWMFTVAGGLLLLSGLGVWVDHAASVQKLIQFGWPAKLA
                     ETIQSNMTFALFYGAGMCAVGYVACLGLERGWLRKSLPWIVPALVLVDAVFLLAPHYL
                     QRMPRSFIEKNILSDFLKGDLSDNRMTFQPQDGIYNHWLTYLFPYQSIATINVTQMSR
                     MPEDYSRFFQQAGRDPVRMWELAAVSHVVGPAGLLGEFERNPAWHGKFEKALDFNVYN
                     DGLGGFSIRPAAADRPGQHAILRFKDRPPRASLIHQWAVLEDEAALQRLFASDFQPGR
                     MAVLSPQAKDLWPDMPESADLTAGTVKIVNSRPGRFELEVESPGPALVRVSEHFDSGW
                     KAWVNGKAAPVLRTDYLFLGVPVNAGHQTIRLEYLPAAWPFWLQMVWVPGLLAALISL
                     GLQRKGGA"
     gene            12732..13337
                     /locus_tag="PKI64_03440"
     CDS             12732..13337
                     /locus_tag="PKI64_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE00098.1"
                     /translation="MNPLFAGIYRMLHARPLVDVAYFTAGTLQPVKDRYDDWVATRDP
                     VPWEELKPWLGLNPSVFFVVTPSSICNARCVFCAYPQLVAKGYRGAVMPMETFQRAVW
                     EWESIGGRSLNLTPTVGEVLTDPGFFDKLAFARSTRIPDIRFYTNGLNLHLNENWRKM
                     VDGRPDAILISLGGCDPVSYRRSFGVDGYANLIKGISLFLD"
     gene            13359..13844
                     /locus_tag="PKI64_03445"
     CDS             13359..13844
                     /locus_tag="PKI64_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOE00099.1"
                     /translation="MDVTLAFRPVQSGWRLTDTEDFRRIIRPHLGPRVRYTIKAGYDN
                     WGGVIAQANLPRPMRLRPARRPLSLPCINLFQPAVLPDGTLRCCACRIRDTNRDELEV
                     GNIHESTLADLMKSARFQALLQRFYAGNRPAICQGCSLYRPINRPWFNAFQAAANSPA
                     S"
     gene            complement(13873..14826)
                     /locus_tag="PKI64_03450"
     CDS             complement(13873..14826)
                     /locus_tag="PKI64_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOE00100.1"
                     /translation="MKNLILSTMLVGALLAPEMALAWGPEGHTVVGDIAEHYLTPRAK
                     RQVHKILQENRLGDFEISSWPDNIRRTPEYAERYPGNGSWHYIDFDTWLRYDENFELK
                     PPPDGQNVVDQITRWQKALMSKDLNAEQRLDALRFLVHFVGDLHQPLHCAFRLGDMGG
                     NMIPVNSFTGRNYSFGPETPMDYPPSIHSTWDEYLVLEMMGRQRRPTFAKTLINDISP
                     EQLHWWSQGAPLDWATDSYWRARKQAYRWANGENLPYTWSRPGMDLTSENYIDARVPI
                     AREQLQKAGVRLAHLLNEAFDPGYAAPAKPASKVVKEAAAP"
     gene            14947..16113
                     /gene="lpxK"
                     /locus_tag="PKI64_03455"
     CDS             14947..16113
                     /gene="lpxK"
                     /locus_tag="PKI64_03455"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOE00101.1"
                     /translation="MARSSRERLEIFFLAVISDRLHGAWPSLLRFLLKLLSRVYSLIM
                     NVRLALYEHRIIRSHTLGCQIISIGNLTVGGTGKTPVVEVFARALQQNGRKVAILSRG
                     YKKAQTPFAERFKNWITFKTPAQTPPRVVSDGQRLLLDSVMGGDEPYMLATNLPEVCV
                     LVDKDRVKSGRYAIQKLGCDTLVLDDGFQYLRLKHRMDIVLVDRTNPFDNGYVLPRGL
                     LRESVKHLSRASFIFITKSTGDGSPELRARIRELNAHAEITECRHSPRYLQDVFTGAR
                     HPLEFLQGKKIAALSGIAAPRGFEETLERLGGELVYRKRFADHHRYRPQEILDVLNAA
                     RDRGADLVLTTEKDAVRLPLVDRRDLPILYLRVQIELLSGAEDFDALISRICFK"
     gene            complement(16120..17190)
                     /locus_tag="PKI64_03460"
     CDS             complement(16120..17190)
                     /locus_tag="PKI64_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOE00102.1"
                     /translation="MAALLLYLIGARAAGKTAVFDALVQTPEGPHFATKGGHRLGTVK
                     VPDARLAALRDIFQPQKYIPAEVTFVDVALPANAPGSGFGPLNAFLGEADAFALVVQA
                     FGETDGAGKPLDSVAQMESVLLDLAMADLEKVERRIEKIEQEVKRGLKQGLPELAALQ
                     KCRQALENGQALREVVLSAAEEKMISSFCFLSQKRVLVVANTGESDPHGESCGALRAA
                     CAVRGLDLLAFCAPLEAEIATLPAGEQDSFLQNYGLREPARQRLLQAAYATLNLISFF
                     TVGPDEVRAWTLRRGDNAVTAAGKIHTDLARGFIRAETVAADILLAKGSLAACRESGQ
                     LRLEGKGYVVQDGDVIEIRANA"
     gene            17337..18773
                     /locus_tag="PKI64_03465"
     CDS             17337..18773
                     /locus_tag="PKI64_03465"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOE00103.1"
                     /translation="MQPPRDNFDAFCSILAGLADAAAAFLGVMVAVWIRFDSGWIPLH
                     DQEILPPRIMYLAAAAVLAPLLVIIFRQVGLYIRPQLGPFGDKLPRIVRGVSITLGTA
                     LMLSFIVRPADWPPYSRTVALMSFFSVLLLVLLERFALFRIELHWARRTAPIRRILII
                     GVDDLAARLRRALEREPRLRSRLAGYVLPFEPGTRPITLAAAITPKLVKGHIGELESL
                     IEEENIDDVILADMSISHKRLTEILFHCERNLIPFRMVPDLYRVLTSRVRVNHIGDIP
                     LLGMAAWPLDAFWNRVLKRLEDIAGALVGLVLSAPILLLAAPLIKRSSPGPVFYRQVR
                     CGIGGKEFTIFKLRTMPVDAENATGPVWATPDDPRRTRIGEFLRKWNLDELPQFWNVL
                     VGDMSLVGPRPERPHFVEQFKNDVGHYMTRHVSRPGMTGWAQVNGLRGDTSIADRVQH
                     DLYYLENWSLSFDLKILIQTFFNRQNAY"
     gene            18783..19487
                     /locus_tag="PKI64_03470"
     CDS             18783..19487
                     /locus_tag="PKI64_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE00104.1"
                     /translation="MTKPPLKACVLVPAYNEARNIGAVVREIRDYCPDVIVIDDGSAD
                     ETGQAAAAAGATVLSHVRNQGKGMALQTGFDHAREHGYDLAITMDADGQHAPSDIPAF
                     LKAYERTHSPVLVGNRMGDVARMPRLRRFVNRFMSALLSRVMGQHVPDTQCGFRIYHR
                     SVFPTGPYDATSQRYAAESEILLRIALQGRKIGAVTIQTIYGSEKSKINPFLDTIRFF
                     RMLHRFKAIKKKALAP"
     gene            19484..20188
                     /locus_tag="PKI64_03475"
     CDS             19484..20188
                     /locus_tag="PKI64_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOE00105.1"
                     /translation="MKKFLAALLLASLTPVLATAADAHRSTGQKIAEKLREQGLSKDA
                     AIVAISTLPIVELRGAIPVGHVLFPDTDKTTRLGRDDLQRAGRIFVWAVVGNMLPVPF
                     ILLLLGPVSRLCMKVPVGKRFFDWLFTRTRRKTAEIEKYEFWGLAIFVAIPLPATGAW
                     TGAAAGWLMGIAFWRSMLSILFGVLGAGVIMTALALLGWIGAVIAGIVLTLFFGGIIV
                     QALRKTPAPRGEGSAL"
     gene            20631..21137
                     /locus_tag="PKI64_03480"
     CDS             20631..21137
                     /locus_tag="PKI64_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00106.1"
                     /translation="MIEHRALQRLNGRHAPRSHGYLEAQADGIKSVWLIKDFIPGRIV
                     EAFTPQEIPVVARLMAQLHHHGVAPNDANIANFVRTPAGEILYLDFGRAILWRRAPLR
                     SILYIGHDLAKFRREIFRENLRQYQEFLHHHLAALATPPVRRRAIRLAARYHYFHRSI
                     RKSVISNR"
     gene            21413..22687
                     /gene="nusA"
                     /locus_tag="PKI64_03485"
     CDS             21413..22687
                     /gene="nusA"
                     /locus_tag="PKI64_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOE00107.1"
                     /translation="MNNELLAVFEYMERERKLDRETLAQLVESALQVAGRKSIGRVRD
                     LRVEINRKTLDIKAFASIQVVDFVRNRDEEISLDDALAKYPGKPWKLGDLIEIEVTPR
                     NFGRIAAQTAKQAIIQKLRMAEKDKVYAIYKDHIGSIVTGTVRRFDRSDVFVDLGDAE
                     GVMPGKERVPSEEYQMGDRIRFLLLNLDAQALGTQLTLSRASPEFIKKLFELEVAEIA
                     DGTVEIKGIAREAGFRTKIAVHSRDDKVDPVGACVGLRGQRVKNIVRELFGEKVDIVK
                     WSPDIKTFVTNALAPAKLLRLDVDELENTVKVIVEGEHLSLAIGKKGQNARLTAKLTG
                     WKVDVQKDESDISFEEKIARATDQLAAVDGIGRDHAANLVAAGFLTLEGILAAEIQDL
                     VDVEGFDAEIAQQVRIAAEAAFERENGKAPES"
     gene            22684..>24573
                     /gene="infB"
                     /locus_tag="PKI64_03490"
     CDS             22684..>24573
                     /gene="infB"
                     /locus_tag="PKI64_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOE00108.1"
                     /translation="MRICDLAKELMTTGKELLALLQARGCTAKSTQANLTPELEKYLR
                     DEIQRIYYGGDKAAEAAPAAEPAAEPAPETPAATAPEAPAPATAPAPEPAAEPPAQAE
                     PEAPKVIAIKGHVVVKEFAELLGIKPNILIAKLMKQGIFASIGMKIDFKTAEPIAQEL
                     GFVLEHEKKAPPPQPVKAEPKPEVGPDTKDDLVPRPPVVTFMGHVDHGKTSLLDYIRK
                     SRVAAGEDGGITQHIGAYQVDYQGRLITFMDTPGHEAFTAMRARGANLTDIAVIVVAA
                     DDGIMPQTKEAIQHAQAAKVAMIVAINKCDLRTANPDRVKRQLQQMGLAPEDWGGELI
                     CCQVSATKGDGMDNLLEMILLQADILELKANPRRKAQGYVIESRLEAGMGPTANVLIT
                     NGTLQLNDAVVSGSSWGRIKAMINDQGVRVRTAGPSAAVKLLGLTSVPEPGSEFHVYK
                     NDREARQVAEERAASERLKALTANNEPRAPLTLDELFQEKIGSDKRTLNVILKTDVQG
                     SLEAIKSSLEGIHSDKVELAIIGMGAGNITVNDVLLASASKAVILGFHVGKDAGVGPE
                     AKNKGVELRLYSIIYEMLDDVKNLMTGLLEPIIKEHINGQAVIRQIFDMGKRGKVAGC
                     MCLKGK"
BASE COUNT         4484 a         7774 c         7283 g         4932 t
ORIGIN      
        1 gcgctgcacg aaaccatccg cgacaccggc tgcacgttga aggcgttccg cgcggaatgg
       61 acggcggagt tgccgatgca gttccgcggg atgcaccgtt ttctgcccgc cttgatggcc
      121 atggccggtg cgcgcatcgc ggaaatcccg gtgaaccacc gcccgcgcgc tgccggccaa
      181 agcaaatata ccaactgggg gcggctcaaa gaaaccttgt gggacctgtg ggcggtgcgc
      241 tggatgcaaa agcgctaccg ttcgcgcgcc ctggccagcg attgatccgc gccccgcgcc
      301 cgcgccgagg ggggtgcatt gcttttttgc gcggggcatg gtagggatgg gcacgatttt
      361 ttcccgcaac catggaaacc ttgcgacatt ttctcgatgt cttggccagc ccgatgggcc
      421 tattgggcat ggcagggcaa gtgttgtttt tttcgcgctt tctggtgcag tggatcgcct
      481 cggaaaaaaa gggccgttcc acggtgccgc tctcgttttg gtatctctcc atcggcggcg
      541 gactgctgct gctggtgtat gccatttggc gcaaggatcc ggtcatcacc atcgggcagg
      601 ccgtgggcct attcgtgtat atccgcaatc tgatgctgat ttaccagcac cggggccggg
      661 tcggctgacc ccatgaatac ccccagactg tggcagcttt ttttgctggc gctgttgttg
      721 tggggcggcg aaacgctgcg ccgcgacctg tggaaccccg acgaagtgcg ctatgcctac
      781 atcgcccgcg agatggcgca ggaccaacac tggctggtgc cctttcgcag cggcgaatac
      841 tacgcgcaca agccgccgta tctgttttgg gccatcaatc tggtttcgca cgcaacccgt
      901 atgcccatcg gccggattcc cgtgcgcctg ccggggctgc tggccggcgt gttcgtgctg
      961 tggaccacgg cgcggctggc gacgcgctgg gccggcgcgc gcgcggcgtg gcccgcggtg
     1021 ctgacgctct gcgccaatta catgttctgg cacgaaatcg gctttgcccg catggatggc
     1081 ccgttgctcg gctggaccat ggcagcgctg acgctgctgt ttttgaatga cgaccagccc
     1141 acgctctggc gccccgccgt ggcgtggctc tgcatgggca tgggcgtttt gaccaagggc
     1201 cccgtgggac tgctgacgcc ggccggcgcc tacatcgccg cgcgcctcgc ggccggcgag
     1261 ggccgcctgc tgaaaaaatc gcattggctc tgggggccgt tcctggggct ggcgctgccc
     1321 ggcctctggc tggcgctggc atggcggcat ggcgcaccgc cgggctattt ttcggaattg
     1381 ctgttttcgc aaaacatcca gcgagccgcg ggcattctcg gccaccggca atcgttcgcg
     1441 tatttcttcg gcgtggcccc cgcgcacttt ttgccgtgga cgttgtgcct gccggcggcg
     1501 atcctcgtgc tgcgccgcac accggagcaa cgccaactgc tgcgccggct gctgggctgg
     1561 atcggtttta tttttgtttt tttctcgctc atgacgtcca agcgcaccat ctacattctg
     1621 gggctgttcc cggcgctggc gattttggtg ggcggcactt ggacagaatt ggccacggcc
     1681 acggaccggt gggcgcgctt ggctcgcggc gcgttcttgt ggctgctgac gttggcggga
     1741 ccggcggtgc tcatcgcgag cttcctgccg ctgccaattc cgggccgcgt gttgtggccg
     1801 acaggtctcg cggcgctggc cggcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnntgcc agaaggaacg cagccagccg atttctttca ggccctcggc cagctcaatc
     1981 aggcccacca gccacacgaa ccataacgcg ctgaacatgt gccggcggta ggcgcgcgga
     2041 ttaacggtgt aggtgtcggg ctcgcggaac agcgacaact tgggaaaaaa caccggcacc
     2101 cgttgcacgt agtcgctgaa cgcgctgccg aaatattcct gcaagcggcg ctcttccttg
     2161 cggatgacca acggataata cagcgccatg gcgagcagga tcagcaccgg aaacgtgaag
     2221 gcctcggtgc agcagcccac gcccaacgca cccagcatgc tgaaaaaata gagcggattg
     2281 cggctcatgg aatacggccc ggcggtgatc agcgtgcggt ctttgtagcc ggcaatgtag
     2341 accgagcacc acatgcgccc gagcgaggcc acggccacca gcagcattcc gacggtgaac
     2401 aacatcgtgg tgatcagggg gctggtttcc tcccaccggc tgctggaaaa cagcaggaaa
     2461 aacacggcca cgaccgccag gccgcgcgaa aaaggaatgc ggagtttttc gtatcgagtt
     2521 ctcatgatgg ttccgggggt gaccaattca aacgcgcaag gaatccagca gcagcttcgc
     2581 aaaccgccgc cccggcggca aatctattcg gcaggcttca cccgccgcgg ttgcgggacc
     2641 agccgcgctt ggcggggggc ggctgcaggc aggcctcgcg ccgtgtttcc acatagaggt
     2701 tcaggcccag tttttcgcat tccttggcaa aggcttcgct cacgcgcacg gcaaaggttt
     2761 taccggtggc gatatacact ttcaggctgt ccgggaaaat caggctcagg ttcaccgcgg
     2821 tggtccccgg atggcgccgg agcaattcct gcaacgcctt cattttggca tcctcccaaa
     2881 tgcccacggg aatatgcacc gtggccgaga gcgcaaactt ttcgggcacc atgtcgagcg
     2941 ggtggatttc gtccacggcc agcacattgg cgctgctgcc gttgtccttg cgcacggttc
     3001 cgcaaagcat gaccgcagca tcgggcgtca gcaaaccgcc atatttttca aatgcctccg
     3061 ggaaggccac ggcggaaacg gtgccttcca gcgtgtccaa ctggaacgac atcatggcct
     3121 gcggcgtggg ccgctgcttg gtgggcttgg tgaatcgctt ctgggcgttg atgaccaacc
     3181 caccgatgcg cgtgggtgtg ctttccggca gcgcggcgaa actgttggca tcgcatttgc
     3241 caaacttggc cagcgtccag gtgtgcgcca gcagcggatg gccggaaata taaaaaccaa
     3301 tcagttcttt ttcgtaagcc agcatgtcct tctgcgacca cgggggcagg atgggcaggt
     3361 cgtcatcctg catgtgggcg ccggagctgt ccgcgccggc catttcgaaa agcgaggcct
     3421 gcccggcggc gcggtccgcg cggcgcgtgg cggcatagct cacggcggct tcaatgccgc
     3481 catacagccg gccgcgcatg agctgggtga agtcaaacgc gccgcacttg atcagcgctt
     3541 ccagcgtttt tttgttcact tcgctgctgt ccatgcgctc gcaaaaatcc acaaacccga
     3601 ggaacgggcc gttggcctcg cgctccttca ccagcgcggc aacagccccc tgccccacgc
     3661 ccttcacgcc ggccagcccg taacggatct tgtcttgcag cggggtgaag cgcgcgccac
     3721 tttcgttgac atcgggcggc agtacgggga tgcccatctt ttgcgcctcc gccaccagca
     3781 ccggcagctt gtccgtgttg ccgatttccg acgacagcag cgcggccatg aattccaccg
     3841 gatagtgggc cttcagatag gccgtctgga acgtcaccat gctgtaggcc gcggagtgcg
     3901 atttgttgaa gccgtattcc gcgaaggcgg caatctggtc gaaaatctca ccggctttgg
     3961 ccgcgttgca ggtgcctttc ttgaggcagc catccacgaa cgcctgccgt tgctggcgca
     4021 tctgttcggc ctgctttttg cccatggcgc ggcgcaaaat gtcgccctgc cccatgctga
     4081 aaccggccag cagcccggcc gcctgctgca cctgttcctg atagaccatg atgccgtagg
     4141 tttccttcag gacatcctcc agcaacgggt gggcatagct gatggtctcg cgcccgtgct
     4201 tgcgcgcgat gtacgacgga atggtgctca tcggccccgg ccggtagagc gcgatcatgg
     4261 cgatcaattc ctcgattttg ctgatttgca actgccgcag caggtcgcgc atgccatcgg
     4321 attccacctg aaacacgccc acggtgtcgc cgcgcgcgag gagctggtag gtttcggcat
     4381 cgtccagcgg caggtcgtcg gggttgattt cgatgccgcg agtttgacga atgttgtcgc
     4441 aggcctcgcg caccacggtc agcgtcttga ggccgaggaa gtccatcttc agcaggccca
     4501 agtcttccag cgggccggcc tcccactgcg agatgacatt gccgtcttta tcgcgcgtca
     4561 gcggcaccag ctcaaagagc ggcttttcgc cgatgaccac cccggcggcg tgggtaccgg
     4621 tttgccgggg catgtcttcc agatgcggcg cgtacttcat gatctcgatg gcgtacggtt
     4681 ccgtccggca cgccgtcttg aaatccacgc tggtgttgag cgccttctcc agcgtggtgc
     4741 cgggcacttc cggcaccatc ttggcgaggc ggtcgcattc gcccagcggg aactccaacg
     4801 cgcgacccaa atcgcgaatc aaggtcttgg cgccaagcgt gccgtaggtg atgatttgca
     4861 ccacgctgtc cgcgccgtat ttgcggcgca cgtaatcgag cacttcgccg cgccgcgtct
     4921 ggcaaaaatc aatatcgaag tccggcggcg agatgcgttc gggattgagg aagcgctcga
     4981 agatcaggcc gtagcgctgc ggatcaatgc cggtgatgcc catggcgtag gccacaaggc
     5041 tgcccgcgcc ggaaccgcgc cccggcccca ctggcacgcc catctgtttg gcggcatgga
     5101 tgaagtccca caccacgagg tagtaattca aaaagcccat cttttcgatc acggcaattt
     5161 cgtgattcat gcgctccacg atggtccgtt cgcggtcgtc gcgggggtgc tccggatcat
     5221 ccacgccgta gagccggcgc acgccttccg tggcgatttg cgtcaggtat tgcttgtggc
     5281 tcaggccgcc ggggcattga taattcggga aatgcggatt tttctggcca tccagcgcga
     5341 gggtcacgtt gcagcgctcg gcaattttcc acgtgttctc gatggcttcc ggcagctcgc
     5401 ggaacagctc gcgcatttcg gcttcgttct tcaagtagaa ctggttggag ccgtacttca
     5461 tgcggtcggt ttcgctccac ttggcctgcg tttgcaggca cagcagcatt tcatgcggct
     5521 cgacatggtc gcggctgaga tagtgcacgt cattcgtggc cacaatcggc aaccccagct
     5581 tgcggctcag cgcgaccagc ttgcggttca ccggcccctg ctcaggcaag ccgtgctctt
     5641 gcagttccag ataaaagcga cccggcccca tgatttcagc atattcgccg gcgagttgct
     5701 cggccttgtc gtcaaggcca tccaccaacg cctcggccac ttcccctttc aggcaggccg
     5761 aaagcgcaat gaggccctcg ctgtattccg ccagcagcgc gcggtcaatg cgcggcttgt
     5821 aatagtagcc gtcgagatgc gcgcggctga ccagtttcat caggttttca aatccgcgat
     5881 tattctccgc cagcagcacc agatggttcg cctgccgccc gttcaccagc actttttcgc
     5941 ggtggttgcc gggggtcaca taggcctcgc agcccatgat gggtttgatg ccgtgcttgc
     6001 gcgcggcctt gtaaaaatgg atggcgccat acaggttgcc gtggtcggtg atggccatgg
     6061 ccacctgctt ttgttcgact gcccgctcca tgagggggct gcggtgcttt tcgccccggt
     6121 ccagggcgca cagcccatcc agcaggctgt attcggtgtg caaatgcaaa tgggtaaagg
     6181 ttggttccaa agtggtctct cggggctaaa agtgtatcta aactttctac cccaacccac
     6241 cggcaaatcc aagccttttt caggcagatg aaatattttt tattttatta ttccccaata
     6301 agttatgact tggagggccg gcctgtctat gtttccgtca ccatccccgc caaaccccgc
     6361 caaaacaggc cttttcagcc gcgccgcgcc tcgagctggg ccaggcattc atccggcacg
     6421 ttcaggccgc caaaccccac gcccagccgg atgcccggcg atacctcgcc atggaaatcc
     6481 gagccgccgg taggcaccaa atccacctcg cgcgccatgg ccagatagtc cttggtcagg
     6541 tcggcggaat gttcggaata atagcactcc acgccggcga ggcccgccgc cgcgagttgc
     6601 cgccacagag ccagcatgtc gtcgcgcgaa atccgcaacg tgaacggatg cgccagcact
     6661 gccacgccgc cggcctgacg gatcagcgcc acggccgctt ccgcggtcag tcgcggccga
     6721 tcggcatatc ccggcttgcc gtcaccgagc catttgtcga acacttcgtt tttgtccttg
     6781 gcatagccct tggccagcat cacctgcgcg aagtgcggac gcccaacaac atcctcgcca
     6841 gcgaccgcct gcacttcgtc ccacgtcatg gcaaaaccca acgcattgag tttttccagc
     6901 atggtggtgt tgcgcatgtg gcggccgttg cggatccact ccatctgccg caccaactcg
     6961 gggtgtgcgg tatccatcca gtagcccagc atgtgcatca cgccgaacgc gcaatccacg
     7021 ctgatctcca cgcccggcac cgcgcggaag gagcgcccct cggccgccgc caaaaagcgc
     7081 ggcaggccgg ccacggtgtc gtggtcggtc agggcgaggg ccttcagccc aattttgacg
     7141 gcttcatccg ccagttgctc cggcgtaagg gagccatcgg aaaacgtgga gtggcaatgc
     7201 aggtctatca tgaggcacag ggggtataag tttggttgga tatgggaagg tacaaccggc
     7261 aagcgcgcgc cgccagtccg ccgccgcaat tattggtcgg tcgggagcag gtggcacaac
     7321 gcggcccaga agtcatcagc atcgcggtcg tggtcgtcgg ccgactccgc gaggatcgtg
     7381 tagcgcactt tcaggaaatg gttgtgaaaa gtcgccacat ataaatagga acgatagtcc
     7441 tgcccagcgc gcgagaacag gtagatggcg cgccacacgg gcaggacgcc ggcttccttg
     7501 ctcgccagtt cgccctccga cagaaccttg aagttttcgt agagcccctg ctccgccacc
     7561 ccaataattt cctccgatgc ttgcatgaag tgctctttca ccagcggcgc atgcagatcg
     7621 ccgggaatgg agtcccggcc catcgtgtag agatacacat ccaccttgct ggccggagag
     7681 ccgtagcgca cgctaatgcc gagcccggcc tgccgggctt cgtaatccac cacttccgcg
     7741 tccttgtcca ggccggcgag gacgtccggg aatatgatcc cggttgcctc gtggtggtaa
     7801 ggatcggccc acgcggaaac cgccagtaac gccagcacca ggcagcaact caactgtttt
     7861 gtcttcattg ttttctttcc gaccggccat caggaccggc actcaaaatg aagcagggtt
     7921 caaggacccg gcgttggcct gaatcctgaa ccctgcctcg gcatgatcgg cgccggagcg
     7981 ccggctctgc attattcctt ctggctggcg ccgggggacc attcctccgg attcttgtcg
     8041 ctgctgctca aggcggcatc gaacgcgccg gccaaatcca cgaggtcttc gcccgcgcgc
     8101 aggccttcga gcatgctgtc atccaccttg aagtcctctt ccggcaactt ggcggccttg
     8161 atgctcagcc cgatgcgccg gtcaatcgga tcaatcttga tgacgcgcgc ctcgacttcc
     8221 tggcccggct gcaacacatc gcgaatcttc gtgacgtggt catcgctgat ttggctgatg
     8281 tgcacaagcc cgtccacgcc ttcttccagt tccacgaacg cgccgaagct ggccagcttg
     8341 ctgaccttgc ccttgatgag ctggccaatg cggtagcggt tggaaatgcc cgcccacggg
     8401 tcttcctgcg cctgcttcag gcccaggctg atgcgctgat tttccggcga gacttcgagg
     8461 acgatggcct cgacttcgtc gcccttcttg agcacctcgt tcgggtggct gaccttgcgc
     8521 gcccagctca tgtccgacac gtgaatcatg ccgtccacac cttcttccag ctctacgaac
     8581 gcgccgtagt tggtgaaatt gcgcaccttg cccttcacgc ggctgccaat cggatacttc
     8641 gccgccgcat cgtcccacgg attgacttcc gtctgccgga tgcccagcga aatcttctgc
     8701 tcttccttgt tgacgttgag caccacggca tccacttcct gccccacact caacgcatcc
     8761 gcggcgcgcg ccacgcgctt cgtccacgaa atttcgctga cgtgcacgag gccttccacg
     8821 ccgtcttcca actgcacgaa cgcgccgtag ggcaccaggt tgaccacctt gccgcgaatg
     8881 cgcgtcccct tcgggtactt cagctcgatc tcttcccacg ggttctgctg cttttgcttc
     8941 aagcccagcg aaatccgttc cttgtcgagg ttgacatcca gcacgaccac ttccagctcg
     9001 tcgcccacct gcaccacctc gctcgggtgg ttgatgcggc cccagctcat atccgtaatg
     9061 tgcagcagtc cgtccatgcc gttgaggtcc acgaacgcgc cgaattcggt gatgttcttg
     9121 accacgcccc ggcgggtctg gcccggctgg atttcctgca gcagtttctt cttctgctca
     9181 cggcgggttt cttcaatcag atcgcggcgc gacaacacga tgttgcgacg ctccttgttg
     9241 atcttgatga ccttgcactc cagcgactgg cccagataat catccaagtt ccgcgccggc
     9301 accacgtcaa tctgcgagcc cggcaggaac gcatccacac cgcccacatc caccagcagg
     9361 ccgcccttga ccttgccctt gaccagaccc gtaaccacct tgccttcttc gcacgaggcg
     9421 gtgacgttgt cccagttgcg ttgctgttct gcacgcgtct tgctcagcac gaccatgccg
     9481 tgctcgtctt ccaaacgttc caaatacact tcgacttcgt cgccgacgtg gacttcgctc
     9541 agatccttga attcggaagc ggccaacagg ccttccgact tgtagccgat gttcaccagc
     9601 acttcttgcg ggcgaacttc gaccacgtgc cccttcacaa tcgcgccttc ggcgatcgtc
     9661 ttgaaggttt ctgcatacat cgccatcatt ttggcctttt cggcatccat ttcgacggca
     9721 gggttcgttg ttgatttctt agccatgact cattgagagg ttacgggctc cgggctttct
     9781 tttttccagg tccgcggtcg aaagcccacc gaagcgccag cgaacaggtc ggcccggaat
     9841 tgctccggag ccttgaaaac ggcgcatctg aacacaccgc gcccacgcaa cacaagccca
     9901 attttcagat ttttttcagc tatttatatt ttccgcggcg tgattagtcc ccctttggaa
     9961 gagaaattaa ccgatgtcaa aacgacctct tcgttttttg tcatttgaac cacaatgtcc
    10021 agagtttata aagtctggac attttataaa atatgtgtat ctcatttata tacacattat
    10081 tatatatatt tttacgctga tttttaatcg gttattttga gggggttgtc cacactttat
    10141 aaactctggg cagattttag gtgagcgatt tgtgcctttt tctgtgtgcg gtgtgacccg
    10201 cagggcagtt gccgcacccc aaaccagtac tacaattggc gaagtgccca aaatacttga
    10261 aatggtttgc accgatacga tgaatatgtt tttttgcagg gcatgagcga gcgaatgact
    10321 tggcggcaaa tacttgttgt agcgctgggg tgcctgctgg tcagcttggt ggttttccat
    10381 gtggttctgc cacccggctc gatcctgatg accaccgatg acaacatcgg ccacaacacc
    10441 accgccaaga atatcctgcc gatgggattc cgcgcatggt gggatgccca agccctgtgg
    10501 ggcaccagcg ggatttccgt ttttcggccg atgtacgtgc tgctctattt gctgcccgtg
    10561 gaggtttacc aaaactgggc gcatggcttg gcgctggcgg cggcgtcatt ctggctggtg
    10621 gtgtatctgc ggcgtcgggg ctgctcctgg gccgcgtgcg tcttgggcgg actgacggca
    10681 ttctggaccg gaaacaacct gacgctgacg tatgccgggc acaccaacaa gttcgcaacc
    10741 atggcctttg ccgcggcggc gatcgccttg ctggacaggg cagcgactga tcgtcgctgg
    10801 ggatggggtg cgctggccgg catggcggtg ggcttcatgt tcttcgagca ggcggacgtg
    10861 gctctgttct cggccgtgtt catcgcggcg ttcgggcttt accgcgcggt gcaggcggcg
    10921 gatggctgga aaccttggcc aatggttcgc ctgctgctgc cggcggcggt gattggcgga
    10981 gtcatggtgc tgggggccgt ggggccgtac cagaaagtgg cgaccgaagg catcacggtc
    11041 atgcagcagg acccccaggt gcagtatgac ttcctgacgc agtggagctg gccaccggag
    11101 gagagccttg atttcattgc gccgggtttc accggctggc ggagcgggga ggaaagcggc
    11161 ccgtactggg gccggatggg ccggtcggcc ggctgggaaa ccacccggca gggcttcatg
    11221 aatttcaagc tggaaaacca ttacatgggc gccctgccgg tcctgctggc attgttcgcc
    11281 tttggtggcg cgggagcgca ctggctccgg aaaaagcggg acgcgcgcac tgccgacacc
    11341 ctgttttggg gcttggtttg cgcgggcgcg ctggtgctgg cgttcgggaa attcacgcct
    11401 atttaccggc tagtcgcgat gctgccggga ttttccggga tgcgcaatcc caacaaattc
    11461 ctgcacatat tccagatggc cctggggatc gttgcggcgt ttgggtggga tggtctgctc
    11521 aacggacggc tgggcgaacg gccgggccgc tggatgttca cggtggccgg cggcttgctg
    11581 ctgctgtctg gtctgggggt gtgggtagat catgcggcaa gcgtgcaaaa actgatccag
    11641 ttcggctggc cggcaaaact ggcggaaacc atccagagca acatgacctt tgctctgttc
    11701 tacggcgcgg ggatgtgtgc agtgggatat gtggcctgcc tcgggttgga gcgcggctgg
    11761 ttgcggaaat ccctgccatg gatcgtgccg gctctggtgc tggtggacgc agtatttttg
    11821 ctggcgcccc actacctcca gcgcatgccg cgcagtttca tcgaaaaaaa catcctgtcc
    11881 gattttctca aaggcgacct gagcgacaat cgcatgacat tccagccgca ggacgggatt
    11941 tataatcact ggctgaccta tcttttccct taccagagca tcgccacgat caacgtgacg
    12001 cagatgtcgc gcatgccgga agactattcc cggtttttcc aacaggccgg gcgtgatccg
    12061 gtgcgcatgt gggagctggc ggcggtctcg cacgtggtcg gccccgcggg acttctgggc
    12121 gagttcgagc gcaatccggc ctggcacggc aaattcgaga aggcactgga cttcaatgtc
    12181 tataacgacg gactgggagg attttcgatc cggcccgcag cggcggatcg gccgggtcag
    12241 catgccatct tgcgcttcaa agaccgcccg ccccgtgctt cgctgattca tcaatgggcg
    12301 gtattggagg acgaggctgc gctgcaacgc ttgtttgcgt ccgatttcca gcccggccgc
    12361 atggcggtcc tttcgccgca ggccaaggac ttgtggccgg acatgccgga gtctgcagat
    12421 ttgaccgccg gaacggtgaa aattgtgaat agtcggccgg ggcggttcga gctggaagtg
    12481 gagtcgcccg gcccggccct ggtgcgggtt tcggagcatt tcgattccgg ttggaaggca
    12541 tgggtcaatg gcaaggcggc accggtgctg cggacggact acctcttcct gggtgtgccg
    12601 gtgaacgctg gccaccagac gatacggctt gaatacctgc cggcggcgtg gcccttctgg
    12661 ctgcagatgg tctgggttcc cggactgctg gccgcattga tttcgctggg gctccaacgc
    12721 aagggcggag catgaacccc cttttcgccg gcatctatcg aatgctgcat gcccggccgc
    12781 tggttgacgt ggcctacttt actgccggga cattgcaacc ggtcaaagat cgctatgacg
    12841 actgggtggc cactcgtgat ccggttccct gggaggaatt gaagccttgg ctggggctca
    12901 atccctcggt attttttgtg gtgaccccca gcagtatttg caacgcccgt tgcgttttct
    12961 gcgcctatcc ccagctcgtg gccaagggct accggggcgc ggtcatgccc atggagacgt
    13021 tccagcgggc ggtgtgggag tgggaaagca tcggcgggcg ctcgctgaac ttgacgccca
    13081 ccgtcggcga agtgctgacg gaccccggct tctttgacaa acttgctttt gcccggagca
    13141 ctcgcattcc ggacatccgc ttctacacaa atgggctgaa cctgcacctg aacgagaact
    13201 ggcgcaaaat ggtggatggc cgccccgatg ccatattgat cagcctcggc gggtgcgacc
    13261 ccgtctccta ccggcgcagt ttcggcgtgg acggctatgc caacctgatc aagggcattt
    13321 ccctttttct ggactaagac aagcaacagg gctaccccgt ggacgtgacg ctggcctttc
    13381 gcccggttca gtccggctgg cgcctgaccg acaccgagga ttttcggcgc atcatccgtc
    13441 cacacttggg gccgcgggtc cgctacacga tcaaggcagg ctatgacaac tggggcggtg
    13501 tgattgctca agccaacctg ccgcgcccca tgcgcttgcg gcccgcgcgc cgccccctgt
    13561 cactgccgtg catcaacctg ttccagccgg cggtactgcc ggacggcacg ttgcggtgct
    13621 gtgcctgccg gatacgcgac accaaccggg acgagctgga ggtgggcaat atccacgaga
    13681 gcacgcttgc cgacttgatg aaatcggcac ggtttcaggc ccttctccag cgcttctacg
    13741 caggaaaccg gccggccatc tgccaaggtt gcagcctgta ccggccgatc aatcgcccct
    13801 ggttcaacgc ttttcaggcg gcggcgaatt ctcctgcgtc gtgatccgcg gtgcgacggc
    13861 gcgccgacgg cctcaaggcg cggcggcttc cttcacgacc ttggatgccg gcttggccgg
    13921 cgcggcatag cccggatcga acgcctcgtt cagcaggtga gccaagcgca cgccggcttt
    13981 ctgcagttgc tcgcgggcga tgggcacgcg ggcatcaata taattttcgc tcgtcaggtc
    14041 catgccgggg cgcgaccaag tatagggcag gttttcgccg ttggcccagc gataggcctg
    14101 cttgcgcgcg cgccagtagc tgtcggtggc ccaatccagc ggcgcgccct ggctccacca
    14161 atgcagttgc tcgggtgaaa tatcgttgat gagcgtcttg gcaaaagtgg ggcggcgttg
    14221 gcgacccatc atttccagca ccagatattc atcccatgtg ctgtggatgc tgggcgggta
    14281 gtccatgggc gtttccggcc cgaacgaata attccgcccg gtgaaggaat tgaccgggat
    14341 catattgccg cccatgtcac ccagacggaa tgcgcagtgc aacggttggt gaaggtcgcc
    14401 cacgaagtgc acgaggaagc gcagggcgtc caaccgctgc tcggcgttca ggtctttgct
    14461 catcagcgcc ttttgccagc gggtgatttg gtccaccaca ttctggccgt ctggcggcgg
    14521 cttcaactcg aagttttcgt cgtagcgcag ccaagtgtcg aaatcaatgt agtgccacga
    14581 gccgttgccc ggatagcgct ccgcatattc cggcgtccgg cgaatgttgt ccggccagct
    14641 cgaaatctca aagtcgccca gccggttctc ctggagaatc ttgtgcacct gccgcttggc
    14701 gcgcggggtc agataatgct cggcaatatc gcccaccacg gtatgccctt cggggcccca
    14761 cgccagcgcc atttccggcg ccagaagcgc gccaaccagc atggtggata gaatcaggtt
    14821 tttcatgggg aactcctttc gggaaacacc cacggtacgc caggagaacc ccggcgtcaa
    14881 accgtgcgcc cactttttgt gtggcagcgc ttgacccacc ccccgcccca tcgccatagt
    14941 gcggccatgg ctcggtcatc ccgcgaacgg ctcgaaatct tttttttggc ggtcatcagc
    15001 gaccgcctcc acggcgcatg gccgtcgctg ctgcggtttc tgctcaagct gctgtcgcgg
    15061 gtatatagcc tgatcatgaa cgtgcgcctc gcgctctacg agcaccggat catccgctcc
    15121 cacaccctcg gctgccagat catcagcatc ggcaacctga cggtgggcgg caccggcaag
    15181 acgccggtgg tggaagtctt tgcccgcgcc ctgcagcaaa acgggcgcaa agtggccatc
    15241 ctgagccgcg gttacaaaaa agcccaaacg cccttcgccg aacgcttcaa gaattggatc
    15301 accttcaaga cccccgccca gaccccgccg cgcgtggtat ccgacggcca gcgcctcctg
    15361 ctcgattccg tcatgggtgg tgatgaaccc tacatgctcg ccaccaacct gcccgaggtc
    15421 tgcgtgctgg tggacaagga ccgcgtcaaa tccggccgct acgctattca aaagctcggc
    15481 tgcgacacgc tcgtgctcga cgacggcttt caatatctgc gcctcaagca ccggatggat
    15541 atcgtgctgg tggaccgcac caacccgttc gacaacggct atgtgctgcc ccgtggtctg
    15601 ctccgcgaat ccgtcaagca cctcagccgc gcctcgttca tcttcatcac caaatccacc
    15661 ggcgatggct cccccgagct gcgcgcgcgc atccgggagc tgaacgccca cgcggaaatc
    15721 accgagtgcc gccactcgcc gcgctacttg caggatgtct ttaccggcgc gcgccacccg
    15781 ttggagttcc tccaaggcaa aaaaatcgcc gcgctttccg gcattgccgc gccgcgcggc
    15841 tttgaagaaa cgctggagcg cctcggcggc gaactggttt accgcaagcg cttcgccgac
    15901 caccaccgct accgcccgca ggaaatcctc gatgtcctca acgccgcgcg cgaccgcgga
    15961 gcggacttgg tcctcaccac cgaaaaagat gccgtgcgcc tgccgctggt ggaccggcgt
    16021 gacctgccga tcctctatct gcgcgtccag atcgaactgc tcagcggcgc ggaagatttc
    16081 gatgcgctga tctcgcgcat ctgcttcaag tagaccgcgt caagcgttgg cacggatttc
    16141 gatcacgtcg ccgtcctgca ccacatagcc cttgccctcc agccgcaact ggccgctctc
    16201 gcggcaggcg gccagcgacc ccttggccag caggatgtcc gccgccaccg tttccgcgcg
    16261 gatgaacccg cgcgccaaat cagtatgaat cttgcccgct gccgtcacgg cgttgtcgcc
    16321 gcgccgcagc gtccaggcgc ggacttcatc gggccccacc gtaaaaaagc tgattaggtt
    16381 caacgtcgcg taggccgcct ggagcaaccg ctgccgcgcg ggctcgcgca ggccgtagtt
    16441 ctgcaaaaag ctgtcttgct cgcccgctgg tagcgtggca atttcggcct ccagcggcgc
    16501 gcaaaacgcc aaaaggtcca atccccgcac agcacaagcc gcgcgcagcg ccccgcagga
    16561 ttcgccgtgt ggatcgcttt cgcccgtgtt cgccaccacc agcacacgct tctgcgataa
    16621 aaaacaaaaa ctgctgatca ttttttcttc ggcggcgctc aacaccactt cgcgcaaggc
    16681 ttgcccgttc tccagggcct gccggcattt ttgcagtgcg gccagctccg gcaacccctg
    16741 cttcaacccg cgcttgacct cctgctcaat cttttcaatg cgccgctcca ctttttccag
    16801 gtcggccatc gccaaatcca gcagcaccga ttccatttgc gccacggagt ccagcggttt
    16861 gcccgcgcca tccgtttcgc caaaagcctg caccaccagc gcgaaggcgt cggcctcgcc
    16921 cagaaacgca ttcagcggcc caaagccact gccgggcgcg ttggccggca gcgccacgtc
    16981 aacaaatgtc acttccgcgg gaatgtattt ttgcggctga aaaatatcgc gcagcgcggc
    17041 gaggcgcgcg tccggcactt tcaccgtgcc caaccggtgc ccacccttcg tggcaaaatg
    17101 cggaccctcc ggggtctgca ccaacgcgtc aaacaccgcc gtcttgcccg ccgcgcgggc
    17161 gccgatcaaa tataaaagca aagctgccat ggaatccttg aatcgtgccg atgaaccccg
    17221 tggcgcgtcc acgcgccggg ttcgtcacgg cgcgccagta gaacacaagc cgccgtcgct
    17281 ggcaattctc cggcttgtcg gcaaccggcc gatttcgtat ggtgccgcga tagtctatgc
    17341 aacctccgcg cgataatttt gacgcttttt gcagcattct ggccggtttg gccgatgccg
    17401 ccgcggcgtt tctcggcgtg atggtggcgg tgtggatccg gtttgattcc ggttggattc
    17461 cactgcacga tcaagaaatc ctgccgccgc ggatcatgta cctcgccgct gccgccgtgc
    17521 tcgcgccgct gctggtgatt attttccggc aggtggggct ctatatccgc ccgcagcttg
    17581 gcccctttgg cgacaaactg ccccggatcg tgcgcggggt ctccatcacg ctggggacgg
    17641 ccttgatgct ctcgttcatc gtgcgcccgg cggattggcc accctattcg cgtaccgtgg
    17701 cgctgatgtc atttttctcg gtgctactgc tggtgttgct ggagcgcttt gcgctgttcc
    17761 gcattgagct gcactgggcg cgccgcactg cccccatccg gcgtatcctg atcattggcg
    17821 tggatgatct ggccgcccgc ctgcgccgcg cgctggaacg cgagccgcgc ctgcggtcgc
    17881 gcctggccgg ctatgtgctg cccttcgagc ccggcacccg ccccatcacg ctggccgcgg
    17941 ccatcacgcc caagctggtc aaggggcaca tcggcgaact ggaatcgctc atcgaggagg
    18001 aaaacattga tgacgtgatt ctggcggaca tgagcatctc ccacaagcgc ctgaccgaaa
    18061 tcctgttcca ctgcgagcgc aacctgattc ccttccgcat ggtacccgac ctgtaccgcg
    18121 tgctaacttc gcgcgtccgc gtgaaccaca ttggcgacat ccccctgctc ggtatggccg
    18181 cgtggccgct tgacgcgttc tggaaccgag tgctgaagcg gctggaggac atcgccggcg
    18241 cgctggtggg gctggtgctc tccgcgccca ttctcctgtt ggccgcgccg ctcatcaagc
    18301 gcagctcgcc cggccccgtg ttttatcgcc aagtccgctg cggcatcggc ggcaaggagt
    18361 tcaccatctt caagctgcgc accatgcccg tggatgccga aaacgccacc ggccctgtct
    18421 gggccacccc cgacgacccc cgccgcaccc gcatcggcga attcctccgc aagtggaacc
    18481 tcgatgagct gccccagttc tggaatgtgc tggtgggcga tatgtccctc gtgggcccgc
    18541 gcccggaacg tccccacttt gtcgagcaat tcaaaaacga tgtcggccac tacatgaccc
    18601 gccacgtctc gcgccccggc atgaccggct gggcgcaggt caacggcctg cgcggcgata
    18661 cctccattgc cgaccgcgtc cagcatgatc tctactatct ggaaaactgg tcgctctcct
    18721 tcgaccttaa aatcctgatc cagacattct tcaaccggca gaatgcctac taatccgcac
    18781 gcatgaccaa gccccccctg aaagcatgcg tgttggtgcc cgcctataat gaagcgcgca
    18841 acattggcgc cgtcgtccgc gaaatccgcg actactgccc cgacgtgatc gtaattgatg
    18901 acggctccgc cgacgaaacc ggccaggccg ccgccgccgc cggcgccacc gttctgtcgc
    18961 acgtgcgcaa ccaaggcaag ggtatggccc tgcaaaccgg ttttgaccac gcgcgcgaac
    19021 acggctacga cctcgccatc accatggatg ccgacggcca gcacgccccc tccgacatcc
    19081 ccgcatttct caaggcctac gaacgcaccc acagccccgt gctcgtcggc aaccgcatgg
    19141 gcgatgtggc ccggatgccg cgcctgcgcc gctttgtgaa ccgcttcatg tccgcgctgc
    19201 tctcgcgggt gatgggccag cacgtgcccg acacccagtg cggcttccgc atctatcacc
    19261 gctccgtctt ccccaccggc ccctatgacg ctacttccca gcgctacgcc gccgaatccg
    19321 aaattctgct gcgcatcgcc ctgcaaggcc gcaaaatcgg cgccgtgacc atccagacca
    19381 tctacggtag cgaaaaaagc aaaatcaatc cgttcctcga caccatccgc tttttccgga
    19441 tgctccaccg cttcaaagcc atcaaaaaaa aggcccttgc cccatgaaga aattcctcgc
    19501 cgccctgttg ctggccagcc tgacccccgt gctggccaca gccgccgacg ctcaccgctc
    19561 caccggccag aaaatcgcgg aaaaactgcg cgaacagggt ctttccaagg atgccgccat
    19621 tgtggccatt tccaccctgc ccattgtgga gctgcgcggc gccatccccg tgggccatgt
    19681 gctgttcccc gacaccgaca aaaccacccg cctcgggcgc gacgatctgc aacgcgccgg
    19741 gcgcatcttt gtgtgggccg tggtgggcaa catgctgccc gtgccgttca ttctgctgct
    19801 gctgggcccc gtctcgcgcc tgtgcatgaa agtccccgtg ggcaaaagat ttttcgattg
    19861 gctcttcacc cgcacccgcc gcaaaaccgc ggaaattgaa aaatatgaat tctggggcct
    19921 tgccattttc gtcgccatcc cgctgcccgc caccggcgcc tggaccggcg ccgccgccgg
    19981 ctggctgatg ggcattgcgt tttggcgcag catgctatcc attttgttcg gcgtgctggg
    20041 cgccggcgtc atcatgaccg ccctcgccct actcggctgg atcggcgcag tcatcgccgg
    20101 catcgtgctc accctcttct tcggcggcat cattgtccag gccctccgca aaacccctgc
    20161 cccacgcggt gaggggtcgg ccctatgatt taatgttttg gggttgcgcg agaactcgcc
    20221 gatcttttgc gttgtttcaa tatattgacg ttggccggta ttttcctata aaaacaaaca
    20281 gcagtgaggc tgttttcgag ccggaaaatt gtacggttac aggtttggat ggattacgcg
    20341 gctgtcgata cttttcagca cagtgtggcg tttgctggca ggtgcgggcg ggcgcgtttt
    20401 agtatctcat gattgcgccg catcaaactg aatcaaccaa acatgcgaca gcccctcacg
    20461 cgccaccctt tcataaaatt cagcaccttc tagccacgcg gtcttcctgg cagatcctcc
    20521 atcaccgaca agccaaagaa acccggcgca ccgttttcgt cgcacaagac cgctacatca
    20581 tcaagcagtt tgaaatcccc gaacgctgcc gacattaccg gcggccatgg ttgatcgagc
    20641 accgggccct ccagcggctc aatggtcgtc atgcgccacg gtcgcacggc tatttggaag
    20701 cccaggcgga cggtatcaaa tcggtttggc tcatcaagga cttcattccc ggtcgcattg
    20761 tggaggcctt cacgccgcag gaaattccgg tggtcgcccg cctcatggcc caactgcatc
    20821 atcacggcgt agcccccaac gacgccaaca ttgccaactt tgtccgcacg cccgcaggag
    20881 aaatcctgta ccttgatttc ggccgggcca ttctttggcg tcgcgcaccg ctacgttcca
    20941 tcctgtacat tggtcacgat ctcgccaaat tccgccgcga aatattccgc gaaaatctac
    21001 ggcagtacca agagttcctt caccaccacc ttgccgcact cgccaccccg ccggtgcggc
    21061 gacgcgccat tcgcctggcc gcgcgctatc attatttcca ccgttcaatc cgtaagtccg
    21121 ttatatccaa ccgatagcga ctcagagggg cagtccattg atttaatgtt ttaggctgtg
    21181 cgcgggcggc ggcaacggga cgcggccggg ataaccggcg gaggccgggc gggttgtggc
    21241 caaaaccgca aaattatgga gattggagat tgacggcggg gggggggatg gctagagtcc
    21301 ccggcgttga tacaactctg gcttgtggaa gagtgggttg cccccactct tttgcgtatc
    21361 cggggtgtgg tgtgtttgtt cttttccccc gtgaggggag gaggtttgaa atatgaacaa
    21421 tgaattgctg gcagtttttg aatatatgga gcgggagcgc aagctggacc gcgaaacctt
    21481 ggcgcaactg gtggagtctg cgctgcaagt ggctggacgc aaaagtatcg gccgggtacg
    21541 cgatttgcgg gtcgagatca accgcaagac cctcgacatc aaggcatttg ccagcattca
    21601 ggtggtggac tttgtacgca accgcgacga ggaaatctcg ctggatgacg ccctcgccaa
    21661 atatcccggc aagccctgga aactgggcga tctgatcgaa atcgaagtca cgccgcgcaa
    21721 cttcgggcgg attgccgccc agacggccaa gcaggccatc atccaaaaat tgcggatggc
    21781 cgaaaaagac aaggtgtatg cgatctacaa ggaccacatc ggctccatcg tgacggggac
    21841 ggtgcggcgc tttgaccgca gcgacgtgtt tgtggacctt ggcgatgccg aaggggtcat
    21901 gcccggcaaa gagcgtgtac cgagcgaaga atatcaaatg ggcgaccgga ttcgcttcct
    21961 cctgctgaat ctggacgcgc aggcgctggg cacccagttg acgctgtcgc gcgcttcgcc
    22021 ggagttcatc aagaagctgt ttgaactcga ggtggccgaa attgccgacg gcacggtgga
    22081 aatcaagggt attgcccggg aagcgggctt ccgcaccaag attgcggtgc actcgcgcga
    22141 cgacaaggtg gatccggtgg gcgcatgcgt cggtttgcgc ggccagcggg tcaagaacat
    22201 cgtgcgcgag ctgttcggcg agaaggtgga catcgtcaag tggagcccgg acatcaagac
    22261 gttcgtcacc aacgcgctgg cgccggccaa gctgttgcgg ctggatgtgg atgaactgga
    22321 aaacacggtc aaggtgatcg tggagggcga gcacctctcg ctggccattg gcaagaaggg
    22381 ccaaaacgcc cgcctgacgg ccaagttgac cggctggaaa gtggatgtgc aaaaggacga
    22441 aagcgatatc tccttcgagg agaaaatcgc ccgggcaacc gaccaattgg cggcggtgga
    22501 cggcattggc cgcgaccatg cggccaatct ggtggcggcc ggcttcctga cgttggaagg
    22561 cattctggcc gcggaaatcc aggacttggt ggacgtggaa ggcttcgatg ccgaaatcgc
    22621 ccagcaggtg cgcatcgcgg cggaggccgc cttcgagcgc gaaaacggaa aggctcctga
    22681 atcatgagaa tctgcgatct agccaaagaa ttaatgacca ccggcaagga gctgctggcg
    22741 ctgctccagg cccgtggttg tacggcgaaa agtacgcagg ccaacctgac gccggagttg
    22801 gagaaatatc tgcgcgacga aattcagcgc atttattacg gcggcgataa agccgctgaa
    22861 gcagcgcccg cggccgagcc ggccgcggaa ccagcgcctg aaacgccggc ggcaaccgcg
    22921 ccggaggccc ccgcccccgc cacggctcct gctccagaac cggcagccga gccgccggca
    22981 caggcggaac cggaagcgcc caaggtcatt gccatcaagg gccacgtagt ggtgaaggag
    23041 tttgcggagt tgctgggcat caagcccaat attctcatcg ccaagctgat gaagcagggc
    23101 attttcgcct ccatcggcat gaagattgat ttcaaaacgg cggaacccat cgcgcaagag
    23161 ctcggctttg tgctggagca cgaaaagaaa gcgcccccac cccagcccgt caaggccgag
    23221 cccaagccgg aagtgggacc ggacaccaag gacgacttgg tgccgcgacc gccggtggtg
    23281 acgttcatgg gccacgtgga ccacggcaaa acatcgctgc tggattacat ccgcaagtcg
    23341 cgcgtggcgg cgggcgaaga tggcggcatc acgcagcaca tcggtgcgta tcaggtggac
    23401 taccaagggc gcctgatcac cttcatggac acgcccggcc atgaggcctt cacggcgatg
    23461 cgcgcgcgtg gcgccaacct gacggacatc gccgtgattg tggtggcggc ggatgacggc
    23521 atcatgccgc aaaccaaaga agccatccag cacgcgcagg cggccaaggt ggcgatgatt
    23581 gtcgccatca acaagtgcga tctgcgcacc gccaaccccg accgggtgaa acgccagctc
    23641 cagcaaatgg gtctggcgcc cgaagactgg ggcggcgaat tgatttgctg ccaggtcagc
    23701 gccaccaagg gcgacggcat ggacaacctg ctggaaatga tcctgctgca agcggacatt
    23761 ctcgagctga aggccaatcc gcggcgcaag gcgcagggct acgtcattga gtcacgcctc
    23821 gaagcgggca tgggaccgac ggccaacgtc ctgatcacca acggtacgct gcaactgaac
    23881 gacgccgtgg tttccggttc ctcgtggggt cgcatcaaag ccatgatcaa tgatcagggc
    23941 gtgcgcgtgc gcacggccgg accatccgct gccgtcaagc tgctcggcct gaccagcgtt
    24001 ccggagcccg gcagcgagtt ccacgtgtac aagaacgacc gcgaagcacg gcaggtggcc
    24061 gaagaacgcg ccgccagcga acggctgaaa gctctcaccg ccaacaatga gccgcgtgcg
    24121 ccgctcaccc tcgatgaact cttccaggaa aaaatcggca gcgacaagcg cacgctcaac
    24181 gtgattctca aaaccgatgt gcaaggctcg ctcgaagcca tcaagtcgtc gttggaaggc
    24241 atccatagcg acaaggtcga gttggccatc atcggcatgg gcgcgggcaa catcaccgtg
    24301 aacgacgtcc tgctggcctc ggcttccaag gcggtcattc tgggtttcca cgtgggcaag
    24361 gatgccggcg tgggtcccga agccaagaac aagggcgtgg agctgcggtt gtactcgatc
    24421 atttatgaga tgctcgacga cgtgaaaaac ctgatgaccg gcctgttgga accgatcatc
    24481 aaggagcaca tcaacgggca ggccgtgatc cggcaaatct tcgacatggg caagcgcggc
    24541 aaggtggccg gctgcatgtg cctgaagggc aag
//
LOCUS       DAOPNP010000023        24562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2577_length_24562_cov_8.802832, whole genome shotgun sequence.
ACCESSION   DAOPNP010000023 DAOPNP010000000
VERSION     DAOPNP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24562
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2577_length_24562_cov_8.802832"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..164
                     /locus_tag="PKI64_03495"
                     /pseudo
     CDS             54..164
                     /locus_tag="PKI64_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
     gene            complement(315..1793)
                     /locus_tag="PKI64_03500"
     CDS             complement(315..1793)
                     /locus_tag="PKI64_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOE00109.1"
                     /translation="MNVSHNALYKNFMEWVAGPTGSITLHVLAIVLLFLFASQTTKEK
                     TSEIEVQVVEIDPLKLDTLVPQDPRTELPPETVPTVTPPEVSIDMPPPPEISDFAQAP
                     ADTLTELYIDNAISPIILRGLPPGAMGNRIGESRIGALGNYAGPWGERAEIAVLRALE
                     WLRIHQNADGSWGNNDKEAYTGLAILTYLAHGETTASEKYGATVERAIRFLVARQNEQ
                     GEFLKTEGSGGAYGHAICTYALSEAYGMTRIPSLRQVMEKAVQVILDGQRNHGGWVMY
                     SFGKTGDAARWDVSLSGFCMQALKAAYIAGADNQGLKASMDKASEFLVQRQQQDGSFP
                     YSAANSGRQPNMTAVSVLCLQLLGYGNHSATKSGLSFLKDADCNWKKPEQAPIYSWYY
                     VSQAKFHQGGAHWTAWNNKFAPTLIHNQSSDGSWTSPGHALTGDGVTREFRGESINSQ
                     VYATTLAALTLQVYYRFLPTYQPIETKAIDQKSAEDVRVEIL"
     gene            complement(1915..3063)
                     /locus_tag="PKI64_03505"
     CDS             complement(1915..3063)
                     /locus_tag="PKI64_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00110.1"
                     /translation="MKVLFVNRMACLERGGGETFDLEISRHLARLGVEITLLSGAPLC
                     GPPLLPLPGAKVLHTPWLKRFPWDKVKGGWRIRQLEFEWFEWRAARWIARRAAEWDII
                     QICELPNLVYWLKKRGVATPLVMRLTAPNFYDPRSGAPLADALIASGTTIEKLRARGG
                     ADSASPLPAPTNIPNAVDADLFRPQPSAFRAQHGICRDEFVALYVARFQAFKNHALLL
                     DAFAEFAKTHPAARLVLVGSGPLRERTEQQAATLGISKQVLFLGEVPFAELPAVYAAA
                     DIKVIASDYESFCFAAIEGMSAGLPVLTTDCAWVPRLVADGAGIVVPVGDATALAAGL
                     TKLADNPALRQRLGAAGRDLVLARHTWPASAQKLLDLYQRLLAGAAAP"
     gene            complement(3220..4701)
                     /locus_tag="PKI64_03510"
     CDS             complement(3220..4701)
                     /locus_tag="PKI64_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOE00111.1"
                     /translation="MNVSHRALYKNFMEWAAGPTGSITLHVVAIVLLFLFASQATKEK
                     TSEIEVQMVEIDQQELDDLLEDRPPEELPDMVDTVTPPDVNMDMTPPPDIQDFAAAPV
                     ADTVTELDIASDAISPIIMKGLAPGQMSNRSGAGRNAAIGNYGGQWGQYAEAAVLRAL
                     EWLRINQNPDGSWGTNDKEAYAGLAILTYLAHGETTASEKYGPTVERAIRYLVARQNE
                     QGEFLKTEGSGGVYGHAICTYALSEAYGMTRIPSLRQVMEKAVQVIIDGQRNHGGWVM
                     YSFGKSGDVARWDVSLSGFCMQALKAAYIAGADNPGLKDSMDKAADFLIKRQRQDGGF
                     PYSAANPGRQPNITAVSVLCLQLLGYGNHNATKAGLSFLKDADCNWNKPEQAPIYSWY
                     YVSQAKFHQGGAHWSSWNNKFAPTLIRNQSPDGSWTSPGHALTGDGVTREFRGEGINS
                     QVYATTLAALTLQVYYRFLPTYQPIETKTIDQKSSEDVQIEIL"
     gene            complement(4736..5149)
                     /locus_tag="PKI64_03515"
     CDS             complement(4736..5149)
                     /locus_tag="PKI64_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE00112.1"
                     /translation="MARKRKRPDVTEPALSMTPMIDVVFQLLIYFIVTTKPIDVMTNL
                     DVFRPAPDKTAKEDQKPPNLVRVGVYQDGYTVNDIPVTLDKLDAALGKVASIDTGQTI
                     MITVSAVSKHGMLVKVLDLCAKNNLRSLSVVSASN"
     gene            complement(5169..5582)
                     /locus_tag="PKI64_03520"
     CDS             complement(5169..5582)
                     /locus_tag="PKI64_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE00113.1"
                     /translation="MKKQYPDAELNMTPMIDVVFQLIIFFVTTADLQAKAMDTAVRMA
                     MAPHGPVEEVKDPRTVVVDVDKNGKISIMKTNLSEAQLLNVLVKARNTTGQSTPVVIR
                     GDGETQHDAIKRVMDVCGKAGLWKLRFAALKEKAG"
     gene            complement(5610..6404)
                     /locus_tag="PKI64_03525"
     CDS             complement(5610..6404)
                     /locus_tag="PKI64_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE00114.1"
                     /translation="MSKKLQLTLGILVLAALVLVPLATLAQEEAAAPAGELVMEGSGG
                     GEAAAPSSGLWQVIVGSGWLGVVLWLGLLATSIAAAWLVIDFFITVSPKRIIPEALVN
                     DVREAMEQGDVIKAMQRCEQDQGPLSAILLAGFRNVKEGFEVIQEQVGVAADLESEKM
                     MQRVSYLNMNGNIAPMLGLLGTVQGMIMAFANLANTQAGAAQQAMLALNIAQALWTTA
                     AGLVVAIPAVSFYTYFKNRLSRIILNMEGLTMDLIKALRNVEVVEE"
     gene            complement(6435..7223)
                     /locus_tag="PKI64_03530"
     CDS             complement(6435..7223)
                     /locus_tag="PKI64_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00115.1"
                     /translation="MKTMRILGICVALAMLAGTVWAAPYVVLNDGKQITGTAIRAQPN
                     GDITLMTDMGSRTFPKGSYQRAVADKPAEIDQAAAAIKAQKYDAAKSLLEGVIAKYRH
                     LGWDVEAAKLLGQALLAKGDAEGAVKAYEQLFQLSPAEKQNADSAWGMRTAMLKAKQY
                     PALLRQLDAVAASGSRTDAARAQTMRGDIQLDQNNVELAALDYLRTAILFADVRDPMI
                     MGEATYKAAAALEQLRDPRAKDMYKKVVNEYGSSPYAAQARGKM"
     gene            complement(7241..10072)
                     /locus_tag="PKI64_03535"
     CDS             complement(7241..10072)
                     /locus_tag="PKI64_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE00116.1"
                     /translation="MKKSKWVGTVVMATVMLAGWGARADVDEDFKFASGLVNYQPPFA
                     DFAQKVVDAVLAKDPSQKDRSRIIQAEILIQRRQYADAEAIINEMGMSNPKAQAISLA
                     LARNYFGIGEYDKARALYDAFFKLYENQKPTDADVARFYRDAAYQYAQMLQMISQFEE
                     AAKSFKRVEEVADGKEMQRSMQVHRAQALVRAAENKSGDDKNKLLDEARKLCETIQWG
                     GMDLQFVDSIVVLANIELAKGNPAGAKKVLMEYMDVIKPIDENLKEMGIPLKESPMAG
                     TRSLLGRLFREEADKLAGQAGKEEEAIKAYSGALGEYYNVFIKYGDSEWGPSAGMAAK
                     EIKEILETKYGKQVKIDLPTTLANKAAGTEFMMADNMFRSKQYADAAAEYLQVLSRFP
                     ESGELSIAAVANLLQCYQNLDDSLYAKVVANYLGERFAAKSDIPAKGLVSAAQLYDRA
                     GNAEMSKYLFDRYLAYCPQDSRAGQILFYLASKAEQAGDQDTANNYLAKIITDYPNDQ
                     NYPKALSKRAWKAYADQDYAGAIDGMKLYLESSQPSPTRAQAMFALGDCYRRTDQLAE
                     AARQFNALIAAISPAGNPYGNSAADIQRNATLLEQARFYFAYSLSRLPNDATRKAAIQ
                     KLDEFLGFYPKSDLAAKALNLKGSLQMALKDPGANDTYARLAAEYPNTDEGKNAQYAR
                     ISGALELAQYAQAKDALTAMLASPGSYKVEEFARVGQAMLEHKQWAEAAGAFGQVVGK
                     TEERAVLERALYGMGAAKYELGDYADSVAALNDLMTRWPNSALFYQAKFTLARANLKL
                     GSLPAAKTALNDIFKYAREPEVVNDASLLYAQILEQEKDLVGALATYKRMEYFGSQSM
                     KTERERQQVATAILAAIDVANELDRSADVLESCDRYLQLYPTSPKINEVRQKRTTASF
                     KVNAEVAAPAAVEPAAPAEPTAPAAETEM"
     gene            10131..11291
                     /locus_tag="PKI64_03540"
     CDS             10131..11291
                     /locus_tag="PKI64_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE00117.1"
                     /translation="MTCLYLFNRTIVNPEDIFFAEPTMLYKEYGSTGKRISVIGCGGM
                     RLPTPHDHAAGIAILHAARRAGINYFDTAPFYCDDQSEAIFGEALRTMPPSPHPLYLS
                     SKCSHANGADFRRGLEKSLRRLGVPRIDFFHIWCVMDAADWQARLRGGALRAALRAKE
                     EGLIGHICVSAHMSGPEIEDMIRQHPEIEGLTLGSNAINFPYRQQGVTAAHRHHRGVV
                     AMNPLGGGIIPQHPAAFDFIRSPDDPDVVTAALRFLISDPAITSALIGFHSLEEVAAA
                     VRAVEQFAPYPPTHLDAIRRRLTSDFNEMCTGCGYCLPCPAGIPIPRLMDIHNLMQLG
                     ATDRAIHDRFQNHWELTPAQAADCTACGQCESRCTQHLPIIQRLKKIAAFPL"
     gene            complement(11541..12851)
                     /locus_tag="PKI64_03545"
     CDS             complement(11541..12851)
                     /locus_tag="PKI64_03545"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOE00118.1"
                     /translation="MIWWLYNLLFPIAFAFLLPHFIWRMLRRGGYARDFTQRFAWYNA
                     ATAELLAAPGRPIWIQAVSVGELAVAFSFMDELRRRAPNLRFVLTTNTSTGHALALKK
                     VHAPDVTLYFPMDVPGVVTRALRRIRPSAVVLIENEMWPNLIRYSRKRGIPVLLVNGR
                     ISAHSFKGYRKLRFITRRLLPRLNWFCVQSEVDRDRLLALGAPPERVEIAGSAKYDIG
                     PTAPTAEARASALLAKLGVKPGHPVLVGGSTWAGEEAVLLDFYQTAKATHPGLLLVLV
                     PRHAERREEVLDEIRRRGLHYVQRSQFPDDAPVPEQRPDVLLVDTTGELRGFYAAADL
                     IFVGKSLTQTGGQNPIEPARDGKPVVVGPHMENFAVIADDFTAAGAWRQARDTADLHR
                     ILLELLDDPAARNRLGRAAADLVANRAGATRRMAERVLAERLPD"
     gene            complement(12848..14056)
                     /locus_tag="PKI64_03550"
     CDS             complement(12848..14056)
                     /locus_tag="PKI64_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE00119.1"
                     /translation="MTFEYKGFDAAGRAQSGLIEAADLKDARKRLTVRGILPESVQAA
                     RGTAARRGARATIFSSDVRTMFYRELAALLGSGVALVPALDVILQAPELDQARTLLAA
                     VRDMVREGRPLSAAMPAVSPQVTLFEQAVIEVGERTGGMPEALARLAAFLEEQEKLRE
                     KLMTALLYPLVVVSLALIIGGLVLTLLLPKVQSLFTEANLQLPALTRILLAGGRTLGL
                     LLLLLVAGAVAAFFVLRRRWRTDEVWRIRLDRRLFSLPIIGGGWRNLASLRFVRVFGL
                     LLERGVSLIESVPMAGRASGSAWLASCAARETEAMGQGKPLVAVIRSIQPLHPSLAAW
                     VQAGEAGGNLVGLLDNAAQRFQQSWDRLATRGMLLLEIGLTLVVGLFVTLIALAVLLP
                     ILQMNKGFVP"
     gene            complement(14081..15628)
                     /locus_tag="PKI64_03555"
     CDS             complement(14081..15628)
                     /locus_tag="PKI64_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE00120.1"
                     /translation="MSAAAPGRPALERIADEWLDPALIETVPVDWVRRHGLVPLRWNG
                     AVAIAGAENAALQAYEDLSLLLGVEAVWVTAPAAEIQRAIDRCYFRRAGQTDEAAEQA
                     GQAAVAGGAGTPAAREADDLLAGHADAPVANYVNLVLLEAVKRGASDIHIEPFANTLT
                     VRYRIDGLLYEQPRPPRHLEGALTARLKVMAKLDLAEKRLPQDGVARVRVGAREIDVR
                     VSSVPVAEGERIVLRLLHRESTRYSLNELGIAPAMLAQFRDILREPHGIVLVTGPTGS
                     GKTTTLYATLRELDTAHLNVLTIEDPIEYQLPNIGQVQVHPKIGLTFARGLRHLLRQD
                     PDVVLVGETRDLETAEIVVRASLTGHLVFTTLHTNDAVGAMTRMVDMGIPPYLLAAAS
                     RAMLAQRLVRRLCPNCRRETTLTATDAAVFGQPALAGRPAWEPSGAGCAECLGGYKGR
                     TGIYELLAVTPPLAEAIRKNVPPAELRRQAVAAGFRDMLDDGLDKILAGVTSVAEVLR
                     AAGTRRD"
     gene            complement(15625..17700)
                     /gene="gspD"
                     /locus_tag="PKI64_03560"
     CDS             complement(15625..17700)
                     /gene="gspD"
                     /locus_tag="PKI64_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOE00121.1"
                     /translation="MNKHKQGGAWARVAAGALLGAAWATAGWTQAPEPTLLAPAYEAA
                     GAATEPVAPVAPLSSAAKVQFSFEQADIRLVTQIVGKYINKKFVVPEGVTGRVSILVP
                     EPVPVSEIYPLYLNALEASGYTVVERAGVCQVTPLPAGPSLPASLDAADGLVTKLIPL
                     QHVSAVHVKKSFEGLVRGGREGGLEVFAPGNHLIVTDTAGNVARLEALVAQIDQPGTG
                     NVIEVVPLKYASVEAVAEQLRATIGATDSAVNKINRHFEQVAGGTGSLPTDFTVVPVP
                     QANSLILVGLPMQIAETKGIIQKMDVENPHGIGRLNAIFLNYLSAEEAAKSLNALLGK
                     APAADGLPAQRDIAIEPSIANNALLVDASPQNFEYVRRLVERLDVVPQQVLVEVLIAE
                     VNLEKGRDVSVEWFGLDQPTGKGTGGFGRSRYGTADAMFNLLTNFAFPQGFVGGIISG
                     TFTAPDGTVYNQIPLFLRAVAGEHNLKILSNIPLWAQNNLEASVSVVENIPILKSSVE
                     GSGSGRDYIQNIERQDVGIKLTLTPHVNPEREIQLDLNPSIESIVQQSDPALLYTPTI
                     AKREVKTSVTVPDRATVVISGLIREDNTRIVSKIPVLGDIPLLGALFRSSSISKRRTN
                     LLIFVTPRIVTDMHAAEAERERLERATSLQDATTALDAPDPETAPAPAPPPKRGQKQK
                     RAAPAGDQP"
     gene            complement(17697..18473)
                     /gene="lpxA"
                     /locus_tag="PKI64_03565"
     CDS             complement(17697..18473)
                     /gene="lpxA"
                     /locus_tag="PKI64_03565"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005383064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOE00122.1"
                     /translation="MGVSIHPLAVVHPNAELGADAVVGPFCMVGENVRLGDRVVLRSH
                     VSVEGHTTLGAGCEVWPFASVGGKTQDLKYKGGAPRLIVGEQTVIRECATLNCATFDG
                     GETRVGAKCLIMAYCHVAHDCVVGDRVIMANNATLAGHVIVEDEAIIGGLTGVHQFVR
                     VGRMSIVGGFTKVVKDVPPYMMADGDPLKVYGPNKVGLERHGIPLEVQQALKEAYKIV
                     YRSDLTVAAALEKIVATVATSAPIEHFVDFIRKSERGISR"
     gene            complement(18476..18910)
                     /gene="fabZ"
                     /locus_tag="PKI64_03570"
     CDS             complement(18476..18910)
                     /gene="fabZ"
                     /locus_tag="PKI64_03570"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOE00123.1"
                     /translation="MNTVLEAKDILNILPHRHPFLLVDRIVECNNKDWIVGIKNVAAN
                     EPCFQGHFPGLPIFPGVLQLEAMAQTAGILLNTVVGSAGKVSYYLGVDQAKFRRMVVP
                     GDQMRMEIKLLRLRMGMAKVAGRTLVDGELACEAEMMFGGGK"
     gene            19056..20357
                     /locus_tag="PKI64_03575"
     CDS             19056..20357
                     /locus_tag="PKI64_03575"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOE00124.1"
                     /translation="MSQHHRRWFLKEVPSLLADGVIDADTARRLEARYTTAEPGRNRA
                     LTVFSILGGTLVGLGIILLLAHNWAELSRATRTALAFAPLICAQALAVWLIRSGRDSA
                     AWREGVGLFWTFAIGAAIALVAQTYHIPGDAGRFAMTWMLLTLPVIYLLNASGPALVY
                     LAGMLFWAADTQDNGGAAWVFWPLAALLLPHTYPAVRQNPYGSRAAVLLWGGTVALCI
                     ATAITLEDLVRDRWVFVYAVLFAALYLAGENWLADATSNWQRPLRTIGGGGCVVLTFQ
                     LTYDWMWDDFTWLFWTNHLQNNYAAILSNLILALMAVAVIALLVRSARHGKTHALFIG
                     ALPIMVGLGNFIAHKFGGGAICLANLYAFGLGLDLLIRGFRTRRNGTVNLGLLLLFTL
                     ILLRFWDERFGFVLRGVIFVVLGLAFLSVNLFLARHKKATP"
     gene            20354..20905
                     /locus_tag="PKI64_03580"
     CDS             20354..20905
                     /locus_tag="PKI64_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HOE00125.1"
                     /translation="MKKSTLLTLLALVIGLQLAIPGGMILRREYVLSHGRAFKFRVGP
                     VDPHDPFRGRYVALRFAQNSVAIPPGLTIRRNQTVHVLLGEDDNGFAIFTGLQLTRPK
                     SDPYLTTRVNSAYSGNAILRLPFDRFYMDEKSAPAAEIVARNSLADTNRPTYAEVRIS
                     RGFAVLADLYVADRPIREVLAAP"
     gene            21038..21943
                     /locus_tag="PKI64_03585"
                     /pseudo
     CDS             21038..21943
                     /locus_tag="PKI64_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(22065..>24562)
                     /locus_tag="PKI64_03590"
     CDS             complement(22065..>24562)
                     /locus_tag="PKI64_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOE00126.1"
                     /translation="VHDRIRFKNPDYQRETKYGVKDQSVITSTVGRVFFNEIWPDNLG
                     FFNAPVPKKKLEELIRHCYQVAGHEETVRTLDRVKDLGFEHATLAGISIGLVDMIVPE
                     AKARTIEAARKSLAEVDAQYRKGVITDGERYNKIIDIWTHASDEITGQMFRAMEFNES
                     RNYVNPLFMMVDSGARGNRQQIRQLAGMRGLMAKPSGEIIERPILANFREGLSVLEYF
                     ISTHGARKGLADTALKTADSGYMTRKLVDVAQDVIVVEEDCGTLNGIEVHAIMQGDEE
                     LVPLPQRILGRTALDDIRDPRSNEMIIAAGNEIDERVAERIRRAELDSVRIRSVLTCE
                     SKRGICAKCYGRNLANGRPAVLGEATGIIAAQSIGEPGTQLTMRTFHIGGTASSTFKQ
                     PEIVLKRDGVLKYDELYTVVGPDNNHIVLNKTGMIVLYDEDGRELERHSVPLGSVLTV
                     ADGESVKKNVPIAKWDPYNVPILSEYAGKVELRDFIMGVTVRKEKDEATGLEDLVILE
                     HRDEIHPQVIIRDLDDPKKVLAAYTMPANARVDVEKGDVVYAGAVLAKTPRKQARTKD
                     ITGGLPRVSELFEARKPKDAADIAKIDGIVELGGKVRGRRQLLVRDKITGDTEEHLIP
                     LNKHIVVYAGDHVRKGQPLTEGPVVPQEILEVCGPQELQEYLVNEVQEVYRLQGVEIN
                     DKHIEIIVRQMLRKVRISEPGDTGFLWGEQVEKQTFNEVNRRVAEEGRKPAEASPLLL
                     GITKASLETESFISAASFQDTTRVLTDAATLARVDHLHGFKENVIMGHVIPAGTGFSM
                     YRNIKLVPLAEPIAVEELLGDQLPTTPTTQTEG"
BASE COUNT         4447 a         7931 c         7218 g         4966 t
ORIGIN      
        1 acgacttgat ctgcccccat aaatagcttc gggtgtccca ttgttgaact caggatgaaa
       61 ccctcgcacc ggtgttggag cccatcaccc gcccggcgct ggtggcgctg gcggtttggg
      121 tgggccagcc gcggctgatt gacaacaccg ttctgcgacc ttgatacttg gaaggtcggc
      181 gaccattcca gcccgacaat ccgacccggc tgcttttgta gggcgggaat ggttgaggga
      241 cgataccttc ccgccggaaa aggctggaag gtctgcgacc gttccagccc tacaatccaa
      301 tctggatgtg atgattacaa aatttcgact cggacgtcct cggcggactt ttggtcaatc
      361 gccttggttt cgatgggctg gtaggtgggc aggaagcggt aatacacttg cagcgtcaag
      421 gcggccaagg tggtggcata gacttggctg ttgatgcttt cgccgcggaa ctcacgcgtg
      481 acgccatcgc cggtgagcgc atggccgggg gaggtccaac tgccgtccga gctttgattg
      541 tggatcagcg tgggcgcaaa tttgttgttc catgccgtcc agtgggcgcc accttggtga
      601 aacttggcct ggctgacgta gtaccatgaa tagatgggcg cctgctccgg ttttttccag
      661 ttgcagtcgg catccttcag gaagctcaag ccggatttgg tcgcgctgtg gttgccgtag
      721 cccagtagtt gcaggcacaa caccgacacc gccgtcatgt tgggctggcg gcccgagttg
      781 gcggcggaat acgggaagct gccatcctgc tgttgccgtt gtacaagaaa ttccgacgct
      841 ttatccatgg aggccttgag cccttggttg tccgcaccgg cgatatacgc cgctttgagc
      901 gcctgcatgc agaaaccgga aagcgagacg tcccagcggg ccgcatcgcc ggtcttgccg
      961 aacgaataca tcacccagcc accatggttg cgctggccat ccaggataac ctgcaccgct
     1021 ttttccatga cttggcgcag cgaggggatg cgggtcatgc cataagcttc gctgagggca
     1081 taggtgcaaa tggcgtggcc ataggcgcca ccggagcctt cggttttcag gaattcgccc
     1141 tgctcattct gccgggccac caagaagcgg atggcgcgtt cgacggtggc cccatatttt
     1201 tcggaggccg tggtttcacc atgggccaga taggtcagga tggccaagcc ggtataggcc
     1261 tccttgtcgt tgttgcccca ggagccgtcc gcattctgat ggatgcgcag ccactcaagg
     1321 gcgcgcagca cggcgatttc ggcacgttcc ccccatgggc cagcgtagtt gccgagggcc
     1381 ccgatgcggc tctcgccgat gcggttgccc atcgcccccg gggggagccc ccgcaaaatg
     1441 atggggctga tggcattgtc aatgtacaac tccgtaaggg tgtcggccgg cgcctgggca
     1501 aaatcactaa tctccggcgg cgggggcatg tctatgctca cttccggcgg ggtaacggtc
     1561 ggcaccgtct ccggtggcaa ttctgtccgc ggatcctgcg ggaccaaggt atcgagtttc
     1621 agcgggtcaa tttcgaccac ctgcacttcg atttcgctgg ttttctcctt ggtggtctgg
     1681 ctggcaaaca ggaagagcag gacaatggcc agcacgtgca gtgtaatgga gccagtgggg
     1741 ccggcgaccc attccatgaa gttcttatat agcgcgttgt gcgatacgtt catgacttcc
     1801 tccagcttta tagtcgcagg tcatccttag acggcatctg gcgaacaggc accggtttct
     1861 atcctactta tacatctttt ttgcgcgtga gtcacgcaat tatttcaagc cggttcaggg
     1921 cgcggcagcg cccgccagaa gccgctgata aagatctaac agcttctggg cgctggcggg
     1981 ccatgtatgg cgggccaaca ccagatcgcg cccggccgcg ccaaggcgct ggcgcaaggc
     2041 cggattgtcc gccagcttgg tcaggcctgc cgccagtgcc gtcgcatcgc ccaccggcac
     2101 cacgatgccc gcgccatcgg ccaccagccg cggcacccag gcgcaatcgg tggtcaacac
     2161 cggcaggccg gcggacattc cctcgatggc cgcaaagcag aaggattcgt aatcgctggc
     2221 gatgaccttg atatcggcgg cggcatacac cgccggcagt tcggcaaatg gcacctcgcc
     2281 caggaacaac acctgcttgc tgatgcccag cgtcgcggcc tgctgctccg ttcgctcgcg
     2341 caacggcccg ctgcccacca gcaccagccg cgctgccgga tgggttttgg caaattcggc
     2401 aaacgcgtcc agcaacagcg catggttttt gaacgcttga aaacgcgcta catacaacgc
     2461 cacgaattca tcgcggcaaa tgccgtgttg ggcgcgaaaa gcggagggct gtgggcgaaa
     2521 caaatcggca tccaccgcgt tggggatatt ggtcggcgcc gggagcggag acgcggagtc
     2581 cgcgccgccg cgggcccgaa gtttttcgat ggtggtgccg ctggcaatga gcgcatcggc
     2641 caacggcgcg ccgctgcgcg ggtcataaaa gttgggcgcg gtgaggcgca tcaccagcgg
     2701 ggtggccacc ccccgttttt tcagccaata gaccagattg ggcagctcgc aaatttgaat
     2761 gatgtcccac tcggcagcgc gacgcgcaat ccagcgtgcg gcacgccatt caaaccattc
     2821 aaactccagt tggcggatac gccaaccgcc tttgaccttg tcccatggga atctcttgag
     2881 ccaaggggtg tgcagcacct tcgcgccggg cagcggcagc agcggcgggc cgcagagtgg
     2941 cgcgccactc aacaacgtaa tctccacgcc cagacgggcc agatgccgcg aaatttcgag
     3001 atcaaacgtc tcccccccgc cccgctccag acaggccatc cgattgacaa acaaaacttt
     3061 catgtccggc cggccatgtc acccagacaa gagggtggcc ccgaaacaat cgtatggcac
     3121 gcgcacatgc tgcctgcctg ctggatcggc attaccatgc cgcgcaattt gcccgaatcg
     3181 ccggttgaag gcgacagaaa tccagtccca ccgcgaggat tagaggattt cgatttggac
     3241 gtcctcggag gacttttggt caatggtctt ggtttcgatg ggctggtagg tgggcaggaa
     3301 gcggtaatac acctgcagcg tcagggcggc caacgtggtg gcataaacct ggctgttgat
     3361 gccttcgccg cggaactcgc gcgtgacgcc atcgccggtg agcgcgtggc cgggggaggt
     3421 ccaactacca tcggggcttt ggttgcggat cagcgtgggc gcgaacttgt tgttccacga
     3481 tgaccagtgg gcgccaccct ggtggaactt ggcttggctg acgtagtacc acgagtagat
     3541 gggcgcctgc tccggtttgt tccagttgca gtcggcatcc ttcaggaaac tcaggcctgc
     3601 tttggtggcg ttgtgattgc cgtagcccag cagttgcagg cacagcaccg acacggccgt
     3661 aatgttgggt tggcggcccg ggttggcagc cgaataaggg aagccgccat cctgccgttg
     3721 ccgcttgatc agaaagtcag cggccttatc catcgaatct ttgagccccg gattgtcggc
     3781 gccggcgata tacgccgctt tgagcgcctg catacagaaa ccggaaagcg agacgtccca
     3841 gcgggccaca tcgccggatt tgccaaagga gtacatcacc cagccgccat ggttgcgctg
     3901 gccatcaatg atgacctgta ccgctttttc catgacttgg cgcagcgaag gaatacgcgt
     3961 catgccgtag gcttcgctga gcgcataggt gcaaatggcg tgaccataga cgccgccgga
     4021 gccttcggtt ttcaggaatt cgccctgttc attctggcgg gccaccaaat agcggatggc
     4081 ccgttcgacg gtggggccgt atttttcgga ggccgtggtt tcgccatggg ccagataggt
     4141 caagatggcc aagccggcat acgcctcctt gtcattcgtg ccccaggagc cgtccggatt
     4201 ctgattgatg cgcagccact cgagggcgcg cagcacggcg gcttcggcat attgccccca
     4261 ctggccgcca tagttgccga tggccgcatt gcgaccggcg ccggaacggt tggacatctg
     4321 cccgggggcg aggcccttca tgatgatggg gctgatggca tcggaggcaa tatccaattc
     4381 tgtgaccgtg tcggccaccg gcgccgcggc aaaatcctga atgtccggcg gcggagtcat
     4441 gtccatgttc acgtccggcg gcgtgacggt gtccaccatg tccggcagtt cttcgggtgg
     4501 acggtcttcg agcaagtcgt ccagttcctg ctggtcaatt tcgaccatct gcacttcgat
     4561 ttcgctggtt ttctccttgg tggcctggct ggcaaacagg aagagcagaa caatggccac
     4621 cacgtgcagc gtgatggagc cggtgggacc ggccgcccat tccatgaagt tcttatatag
     4681 cgctctgtgc gatacgttca tgactttctc cagctatatc gtcgcaggtt ctcgttcagt
     4741 tggatgcgct gaccaccgac aggcttcgca aattgttttt ggcgcacagg tccagcacct
     4801 tcaccagcat gccgtgcttg gacaccgcag acaccgtgat catgatggtt tggccggtat
     4861 caatggaggc caccttgccc aacgccgcgt ccagcttgtc cagcgtgacg gggatgtcgt
     4921 tgacggtgta gccgtcctga tacacgccca cgcgtacaag gttgggcggc ttctgatctt
     4981 ccttggcggt tttgtccggc gcgggccgga agacgtccaa gttggtcatc acgtcaatcg
     5041 gcttggtggt gacgatgaag tagatcagga gctggaacac cacgtcaatc atgggggtca
     5101 ttgacagggc gggctccgtc acatccgggc gtttgcgttt tctggccatg gggtgcttcc
     5161 ttcctgtcct aacccgcctt ttccttgagc gcggcgaagc ggagcttcca cagcccggcc
     5221 ttgccgcaga catccatcac gcgcttgatg gcgtcgtgct gggtctcgcc atcgccgcgg
     5281 atcaccaccg gcgtgctttg cccggtagtg ttgcgcgcct tgaccaacac gttcagcagt
     5341 tgtgcctcgc tcaagttggt cttcatgatg gaaatcttgc cgttcttgtc cacgtccacc
     5401 accacggtgc gcgggtcctt gacctcctcg acggggccgt ggggggccat ggccatgcgg
     5461 acggcggtgt ccatggcctt ggcctgcaag tccgcggtgg taacgaagaa aatgatgagc
     5521 tggaacacca cgtcaatcat cggcgtcatg ttcagctcgg catccggata ttgctttttc
     5581 atgcttgtct ccccgggcgg ggtggcagct tattcttcca ccacttccac gttgcgcagc
     5641 gccttgatca ggtccatggt caagccttcc atgttcagga tgatccggga caagcggttc
     5701 ttgaagtagg tgtagaacga cacggccggg atcgccacca ccagaccggc ggcggtggtc
     5761 cacagcgctt gggcgatatt gagcgccagc atggcctgct gcgcggcgcc cgcctgcgtg
     5821 ttggccaagt tggcgaaggc catgatcatg ccttgcaccg tgccgagcag cccaagcatc
     5881 ggggcaatat tgccgttcat gttcaggtag gacacgcgct gcatcatctt ttcgctctcg
     5941 aggtcggcgg ccacgcccac ctgctcctga ataacttcaa agccttcctt cacgttgcgg
     6001 aagcccgcca gcaaaatggc ggaaagcggt ccttgatcct gttcgcagcg ctgcatggcc
     6061 ttgatgacat cgccctgctc catggcttcg cgaacatcgt tcaccaacgc ttcgggaatg
     6121 atgcgcttgg gactgacggt gatgaagaag tcaatgacca gccatgccgc ggcaatggaa
     6181 gtggccaaca ggcccaacca aagcaccacg cccagccagc cggagccaac aatcacctgc
     6241 cacaggccgg aagacggcgc agcggcttcg ccaccaccgg agccttccat caccaattcg
     6301 ccggcgggtg cggcggcttc ttcctgcgcc aaggtagcca gcggcaccag caccaaagcg
     6361 gccaaaacca gaatccccaa tgtcaactgc agtttcttgg acatgttcat tctccttcag
     6421 ttctttttac attgttacat tttgccccga gcctgcgcgg catagggcga agagccgtac
     6481 tcgttgacca ctttcttata catatccttt gcgcgcgggt cacgcaattg ttccaaggcg
     6541 gcggcggctt tgtatgtggc ctcgcccatg atcatcggat cgcgcacgtc ggcaaacaga
     6601 atggccgtgc gcagatagtc cagcgcagcc agctcgacgt tgttttgatc caactggata
     6661 tcgccgcgca tggtctgggc acgggccgca tcagtccggc tgccggacgc cgccacggca
     6721 tccaactggc gcagcagcgc cggatattgc ttggccttga gcatggccgt ccgcatgccc
     6781 cacgccgagt ccgcgttttg tttctcggcg ggcgacaact ggaacaactg ctcataggcc
     6841 ttcaccgcac cctcggcatc acccttggcc agcagggcct gcccgagcag cttggcggct
     6901 tccacatccc agcccaagtg ccggtatttg gcaatcacgc cttccagcag cgatttggcc
     6961 gcgtcatatt tctgggcctt gatggctgcc gcggcttggt caatttccgc cggcttgtcg
     7021 gccaccgcgc gctggtaaga gcccttgggg aacgtccggc tgcccatgtc ggtcatcagg
     7081 gtaatatcgc cattgggctg ggcgcggatg gcggtgccgg taatttgctt gccatcatta
     7141 agcaccacgt agggcgcggc ccagaccgtg ccagccagca tggccagcgc gacgcagatt
     7201 ccaaggattc tcatagtttt catgctctgg tctcccatgt ttacatttcg gtttcggcgg
     7261 caggcgcggt cggttcggcg ggcgcggcgg gttcaaccgc cgccggtgcg gccacttccg
     7321 catttacctt gaaggaagcc gtcgtccgtt tttgccgcac ttcgttgatc ttggggctgg
     7381 tcggatacag ttgcaaatag cggtcgcagc tttcgaggac atccgcgctg cggtcgagtt
     7441 cgttcgccac atcaatggcc gcgaggatgg cggtggccac ctgctggcgc tcgcgctccg
     7501 tcttcatgct ctggctgccg aagtattcca tgcgcttgta ggtggccagc gcgccgacca
     7561 ggtccttctc ctgttcgagg atctgggcgt agaggaggct ggcatcgttg accacttccg
     7621 gttcgcgcgc gtacttgaag atgtcgttga gcgcggtctt ggcagcgggc aggctgccca
     7681 gtttgaggtt cgcgcgcgcg agggtgaatt tggcctgata gaacagcgcc gaattgggcc
     7741 agcgagtcat caggtcgttg agcgcggcca cggagtccgc atagtcgccc agctcatact
     7801 tggcggcccc catcccataa agggcgcgct ccagcacggc gcgctcttcg gttttgccaa
     7861 ccacctggcc aaatgcgccg gcggcctcgg cccactgctt gtgctccagc atggcctggc
     7921 ccacgcgggc aaattcttcc actttgtagc tgccggggct ggccagcatg gcggtcaggg
     7981 cgtccttggc ctgcgcatat tgagccagct ccagtgcgcc gctaatgcgc gcatactgcg
     8041 cgttcttgcc ttcatcggtg ttgggatatt ccgcggccag ccgcgcatac gtatcgttgg
     8101 cgccggggtc cttcagcgcc atttgcaacg accccttcaa gttgagcgcc ttggccgcca
     8161 gatcggactt gggataaaaa ccgaggaatt catccagctt ttggatggcc gccttgcgag
     8221 tggcatcgtt cggcaggcgg ctgagactat aggcaaagta aaaccgcgcc tgctccagca
     8281 aggtggcatt gcgctggatg tccgccgccg agttgccata aggattgccc gccggcgaaa
     8341 tagcggcaat cagcgcgttg aactgccgcg cggcctcggc caactggtcc gtgcgccggt
     8401 agcaatcgcc cagcgcgaac atggcctgcg cccgcgtggg gctcggctgc gacgactcca
     8461 gatacagctt catgccgtca atcgcccccg cgtaatcttg atccgcgtag gccttccacg
     8521 cgcgcttgct cagcgccttc gggtagttct ggtcgttggg atagtcggtg atgatcttcg
     8581 ccagatagtt gttggcggtg tcctgatcgc ccgcctgctc ggccttgctg gccagataaa
     8641 acaggatttg tcccgcccgc gagtcctgcg gacaataggc caggtagcgg tcgaacaaat
     8701 acttcgacat ctcggcgttg ccggcgcggt catacaactg cgcggcggac accagcccct
     8761 tggcggggat gtcgctcttg gccgcgaacc gctcacccag atagttcgcc accaccttcg
     8821 catacaacga atcatccaga ttctggtagc actgcaacag gttggccacc gccgctatgg
     8881 agagctctcc cgactccggg aaccgcgaca acacttgcag atattccgcc gccgcatccg
     8941 catattgctt cgagcggaac atgttgtccg ccatcatgaa ctcggtgccc gccgccttgt
     9001 tggccaacgt ggtgggcagg tcaatcttaa cctgcttgcc gtacttggtt tcgaggattt
     9061 ccttgatctc cttggccgcc atgcccgcgc tgggacccca ctcgctgtcg ccgtatttga
     9121 tgaagacgtt gtaatactcg cccagcgcgc cgctatacgc cttgatggcc tcctcttcct
     9181 tgcctgcctg cccggccaac ttgtcggcct cctcgcggaa gagccggccc agcagcgacc
     9241 gcgtgccggc catcgggctt tccttgagcg ggatccccat ttccttgagg ttctcgtcaa
     9301 tgggcttgat gacatccatg tactccatca gcaccttctt cgcgcccgcc ggattgccct
     9361 tggccagctc aatgttcgcc agcaccacga tggaatccac aaactgcaag tccatgccgc
     9421 cccactggat cgtctcgcaa agcttgcggg cctcatccag caacttgttt ttgtcgtcac
     9481 cgctcttgtt ttcagctgcg cgcaccagcg cttgcgcgcg gtgcacctgc atcgagcgct
     9541 gcatttcctt gccatccgcc acttcctcga cgcgcttgaa gctcttggcc gcctcctcga
     9601 actggctgat catctgcaac atctgcgcgt actgatacgc cgcgtcgcgg taaaaccgcg
     9661 ccacatccgc atccgtcggc ttctggtttt cgtacaactt gaaaaaggca tcatagagcg
     9721 cccgcgcctt gtcgtattcg ccgatcccga aatagttgcg cgccaacgcc aggctgatcg
     9781 cctgcgcctt ggggttgctc atccccatct cgttgatgat cgcctccgcg tccgcgtatt
     9841 gccggcgctg aatcaggatt tccgcttgga tgatccggct gcggtctttt tgcgaagggt
     9901 ccttcgccag caccgcatcc accacttttt gcgcaaagtc cgcgaaggga ggctgatagt
     9961 tcaccagtcc ggaagcaaac ttgaagtcct cgtccacgtc cgcccgggcc ccccagcccg
    10021 ccagcattac agttgccatc accaccgtgc cgacccactt gctctttttc atggtcgcat
    10081 ccatctccta caaaatggcc tgtctgccag tcgaacgctg ttgcagattc atgacttgtt
    10141 tatacctgtt caaccggact atcgtcaacc cggaagacat cttttttgcg gaacccacca
    10201 tgctctacaa ggaatacggc tccaccggca agcgaatcag cgtcattggc tgcggcggca
    10261 tgcgcctgcc cacgccccat gaccacgccg ccggcatcgc catcctgcac gccgcccgcc
    10321 gcgctggcat caactacttc gacaccgccc ccttctactg cgacgaccaa agcgaggcca
    10381 tcttcggcga ggccctccgc accatgcccc cctcccccca ccccctctac ctctcctcca
    10441 aatgctctca cgccaacggc gctgatttcc gccgcggcct cgaaaaatcc ctgcgccgcc
    10501 tcggcgtccc ccgcattgac ttcttccaca tctggtgcgt catggatgcc gccgactggc
    10561 aggcccgact tcgtggcggc gccctccgcg ccgccctacg cgccaaggaa gaaggcctta
    10621 ttggccacat ctgcgtctcc gcccacatga gcggccctga aatcgaggac atgatccgcc
    10681 agcacccgga aatcgaaggc ctcacccttg gcagcaacgc catcaacttc ccctaccgcc
    10741 aacaaggcgt caccgctgcc caccgccatc accgcggcgt agtggccatg aacccgctgg
    10801 gcggcggcat catcccccaa caccccgccg cttttgattt catccgcagc ccggacgacc
    10861 ccgacgtcgt caccgccgcc ctgcggttcc tcatctccga ccccgccatc acctcggcgc
    10921 ttatcggatt ccacagcctc gaagaagtgg ccgccgctgt ccgcgccgtc gaacaattcg
    10981 ccccctaccc gcccacccat ctagacgcca tccgccgccg cctcaccagc gacttcaacg
    11041 aaatgtgcac cggctgcggc tattgcctcc cctgccccgc tggaatcccc atcccgcgcc
    11101 tcatggacat ccacaacctc atgcaactcg gcgccaccga tcgcgccatc cacgaccgct
    11161 tccaaaacca ctgggaactc acccccgccc aagccgccga ctgcaccgct tgcggccaat
    11221 gcgaatcccg ctgcacccag cacctcccca tcatccagcg cctcaaaaaa atcgccgcct
    11281 tccccctctg aagcccctca tttttccaca ctgtggaaaa catatttcca tgctgtggaa
    11341 taaattacgc tttaggtacc caatttttcc acactgtgga aaacttattt ccacgccgtg
    11401 gaaaaccctc tccgccagcc tgccctcccg cccgaatcag gaccacaaaa gacccatttt
    11461 tatttcactt attgcaatat gtgaattcac ttattgtaat aagtgaaaca atacttggct
    11521 gtgttgctgc gcgttgaatt ttagtcggga agacgttcgg ccaagactcg ctcggccatc
    11581 cggcgggtgg cgccggctct gttggccacg aggtcggcag cggcgcggcc aagccggttg
    11641 cgcgcggcgg gatcgtccaa caattccagc aaaattcggt gcaaatcggc agtgtcacgg
    11701 gcttggcgcc acgcgccggc agcggtgaag tcgtcggcaa tcaccgcaaa gttttccatg
    11761 tggggcccca ccaccacggg cttgccgtca cgggcgggtt caattggatt ttgtccgccg
    11821 gtttgggtga ggctttttcc aacaaaaatc aaatcggcgg cggcataaaa tccgcggagt
    11881 tcgccggtgg tgtccaccag caggacgtcg gggcgttgtt cgggaacggg ggcgtcgtcg
    11941 gggaactgac tgcgctggac gtagtgcagg ccgcggcggc ggatttcgtc gaggacttcc
    12001 tcccgacgct cggcgtggcg gggcaccaga accagcaaca atcccggatg tgtggccttg
    12061 gcggtttgat agaaatcgag caggaccgct tcttcgccgg cccacgtgga gccgccaacc
    12121 agcacggggt ggccgggctt cacgcccagc ttggcgagga gcgcgctggc ccgggcttcc
    12181 gccgtaggcg cggtggggcc aatgtcatat ttggcgctgc cggcaatttc cacccgctcg
    12241 ggcggcgcac ccagagccag cagccggtcg cggtccactt cgctctgcac gcaaaaccag
    12301 ttcaggcgtg gcagcaggcg gcgggtaatg aaccggagct tgcggtagcc cttgaacgaa
    12361 tgggcggaaa tccggccgtt aaccagcaag acggggatgc cgcgcttgcg ggagtagcga
    12421 atcagattgg gccacatttc gttttcgatg agcaccacgg cgctgggccg gatgcgccgc
    12481 agggcgcggg tgaccacgcc gggcacatcc atcgggaaat agagcgtgac atcgggcgcg
    12541 tgaacttttt tcagcgcgag ggcatggccg gtggaggtgt tggtggtcag cacaaagcgc
    12601 aggtttggcg cacggcggcg caactcatcc ataaatgaaa aagccacggc cagctcgccg
    12661 acgctgacgg cctgaatcca gatgggccgg ccgggcgcgg caagcaattc ggcggtggcg
    12721 gcgttatacc acgcaaagcg ctgggtgaag tcgcgcgcgt agccgccgcg gcgcagcatc
    12781 cgccagatga aatgcggcag caaaaaggcg aacgcaatcg gaaacaacaa gttatataac
    12841 caccaaatca cggcacaaat cctttgttca tctgcaagat gggcagcagt acggccagcg
    12901 caatcaacgt tacaaacagg cccaccacca acgtcaaacc gatttccagc agcagcattc
    12961 cgcgggtggc caggcggtcc cagctttgtt ggaaacgctg cgcggcgtta tccagcaggc
    13021 caaccaaatt accaccggct tcgccggctt gcacccaggc cgccagcgag ggatgcagtg
    13081 gctgaatgct gcggatgaca gccaccagcg gcttcccctg ccccatggct tcggtttcgc
    13141 gcgccgcgca ggaagcgagc cacgcgctgc cggaggcccg cccggccatc ggcacgcttt
    13201 cgatcaggct cacgccgcgc tccaacagca acccgaacac cctcacaaaa cgcaggctgg
    13261 ccagattccg ccagccgccg ccgatgatcg gcaacgagaa caagcggcgg tccaggcgga
    13321 tgcgccacac ttcatcagtg cgccagcgcc gccgcagcac aaaaaatgcc gccaccgcgc
    13381 cggccaccag caggagcaac agcaggccga gcgtccgccc gcccgccagc aggatgcgcg
    13441 ttaacgccgg caactgcagg tttgcctcgg tgaagaggct ttgcaccttg ggcagcagca
    13501 gcgtgagcac gaggccaccg atgatcaacg ccagcgaaac cacaaccagc ggatataaca
    13561 gcgcggtcat cagcttttcg cgcaattttt cctgttcttc caaaaatgcg gccaaccgcg
    13621 ccagcgcttc gggcatgccg ccggtacgct cgcccacttc gatcacggcc tgctcgaaga
    13681 gtgtcacctg cggcgagacg gccggcatgg cggcgctcag cgggcggcct tcgcgcacca
    13741 tgtcgcgcac ggcggccagc agggtgcggg cctgatccag ctcgggcgct tgcagaatga
    13801 catcgagggc gggcaccaac gccacgccgg agccgagcag tgcggccagc tcgcgataaa
    13861 acatggtgcg cacgtctgag gaaaaaatgg tggcgcgtgc cccgcgccgg gcggcggtgc
    13921 cgcgcgcggc ctgtacgctt tccggtaaga taccgcgcac ggtgaggcgc ttgcgggcat
    13981 ccttgaggtc cgcggcttcg atcaatccgg actgcgcgcg gccggcggca tcaaagccct
    14041 tatattcaaa ggtcatcacg gcggtgtgcg ctcccggcct ttagtcccgc cgcgtgccgg
    14101 cggcgcgcag cacttcggcc acggaagtca caccggccaa aatcttgtcc aagccgtcat
    14161 cgagcatatc gcggaaaccc gccgcgacgg cctgccggcg caattccgcc ggcggcacat
    14221 ttttgcggat ggcttcggcc agcggcggcg tgacggccag caattcataa atgccggtgc
    14281 gccccttgta gccgccgagg cattccgcgc agccggcgcc cgacggctcc cacgcgggtc
    14341 gcccggccag cgccggttgg ccaaatacag cggcatcagt ggcggtgagt gtggtttcgc
    14401 gccggcagtt ggggcacaag cgtcgtacga gccgctgggc cagcatcgcg cgcgaggcgg
    14461 cggccagcag atacggcggg atgcccatgt ccaccatgcg cgtcatggcg cccaccgcgt
    14521 cgttggtgtg cagcgtggtg aagaccagat ggccggtcaa cgaggcgcgc accacgatct
    14581 ccgcggtttc caaatcgcgg gtttcgccga cgagcaccac atccgggtcc tgccgcagca
    14641 ggtgccgcag gccgcgcgcg aacgtcagcc cgattttggg atgcacctgc acttggccga
    14701 tgttgggcaa ctggtattcg atggggtctt cgatggtcag cacgttgaga tgcgcggtgt
    14761 ccagctcgcg caacgtggca tagagcgtgg tagttttgcc actgccggtt ggcccggtga
    14821 cgagcacaat gccgtgcggt tcgcgcagaa tatcgcggaa ctgggccagc atggcgggcg
    14881 cgatgcccag ctcattcaac gaatagcgcg tggattcgcg gtgcagcagg cgcagcacga
    14941 tgcgctcgcc ttcggcgacg ggcacgctgg acacgcgcac atcaatctcg cgcgccccca
    15001 cccgcacccg cgccacgccg tcctgcggca gacgcttttc ggccaagtcc aatttggcca
    15061 tcaccttgag gcgggcggtc agcgcgcctt ccaaatggcg tggcgggcgc ggctgttcgt
    15121 agagcaggcc gtcaatccgg tagcgcacgg tcagagtgtt ggcgaacggc tcaatatgaa
    15181 tatccgacgc gccccgtttg acggcctcca gcaacacgag gttcacgtag ttggccacag
    15241 gagcatccgc gtggccggcc agcaggtcgt ccgcctcgcg cgcggcaggc gtgcccgcgc
    15301 cgccggccac cgcggcttgg cccgcttgct cggcggcttc gtccgtttgg cccgcgcgcc
    15361 ggaaatagca ccgatcaatc gcgcgctgga tttcggcggc cggcgcggtg acccacacgg
    15421 cctccacgcc cagcagcagc gataaatctt cgtaggcttg cagcgcagca ttttccgcgc
    15481 cggcaatggc cacggcccca ttccagcgca gcggcacgag gccatgccgc cgcacccaat
    15541 ccacgggcac cgtttcgatc aacgccgggt cgagccattc gtcggcaatg cgttccagcg
    15601 cgggtcggcc tggcgccgcg gcgctcatgg ctggtcaccc gccggcgccg cgcgcttttg
    15661 cttctgaccg cgtttgggcg gcggcgcggg agcaggcgcg gtttccgggt ccggcgcgtc
    15721 caatgccgtg gtggcgtctt gcaggctggt ggcacgctcc aaccgctcgc gttccgcctc
    15781 ggccgcgtgc atatccgtca caatgcgcgg cgtgacgaaa atcaacaaat tggtccgccg
    15841 tttggaaatg ctggacgagc gaaacagggc gcccagcagg gggatatcgc ccagcaccgg
    15901 gattttgctg acgatgcgcg tgttgtcctc gcggatcagg ccgctaatca ccaccgtggc
    15961 gcggtcagga accgtcaccg aggttttgac ttcgcgcttg gcgatggtcg gggtgtagag
    16021 cagggccggg tcgctctgct gcacaatgga ttcaatactg gggttcaagt ccaactggat
    16081 ttcgcgctcc ggattcacat gcggggtcag cgtcaacttg atgcccacat cctgccgctc
    16141 gatgttttgg atgtagtcgc gcccactgcc ggagccctcg accgacgatt tcaggatggg
    16201 gatgttttcg accacgctca ccgaggcttc cagattgttt tgcgcccaga gcgggatgtt
    16261 cgaaagaatc ttcaagttgt gttcgccggc cactgcccgc aggaacagtg gaatctggtt
    16321 atacaccgtg ccatccggcg cggtgaaggt gccgctaatg atgccgccca caaagccctg
    16381 cgggaacgca aagttggtga gcaggttgaa catggcgtcg gcggtgccgt agcgcgaccg
    16441 cccgaagcca ccggtgccct tgcccgtggg ctgatccaag ccgaaccatt ccacgctgac
    16501 atcgcgccct ttttcgagat tcacctcggc aatcagcact tccaccagca cctgctgggg
    16561 caccacatcc agccgctcca ccaagcggcg gacatattca aagttttgcg gcgaagcatc
    16621 caccagcagc gcgttgttcg cgatgctcgg ctcgatagcg atatcccgct gcgcgggcag
    16681 gccatcggcc gccggcgcct tgccgagcag cgcgttgagg ctcttggccg cttcttccgc
    16741 cgatagataa ttcaggaaaa tagcgttgag tcgcccaatc ccgtgcggat tttccacatc
    16801 cattttttga atgatgccct tggtctcggc gatctgcatg ggcaggccca ccagaatcag
    16861 gctgttggcc tgcggcaccg gcaccacggt gaaatcggtc ggcaggctgc ccgtgccgcc
    16921 ggccacctgc tcaaagtggc ggttgatttt gttgacggcg ctatccgttg cgccaatggt
    16981 ggcgcgcagt tgttcggcca cggcctccac actggcatat ttcagcggca ccacctcgat
    17041 gacgttgccg gtgccgggct ggtcaatctg agccaccagc gcctccagcc gcgccacgtt
    17101 gccggcggtg tcggtcacaa tcaggtggtt gcccggggcg aacacttcca gcccgccctc
    17161 gcgcccgccg cgcacgagac cctcaaaact ctttttgacg tgcaccgcgc tgacgtgctg
    17221 caacgggatc agcttggtca ccaagccatc cgccgcatcc agactggccg gcagcgacgg
    17281 cccggccggc agcggcgtaa cctggcacac gccagcgcgc tccaccacgg tgtagccgct
    17341 ggcttccagt gcgttgagat acaagggata aatttctgac accggtacgg gctccggcac
    17401 cagaatgctc acccggcccg tgaccccctc cggcaccacg aactttttgt tgatgtattt
    17461 gccgacaatc tgcgtcacca gccggatgtc cgcctgctcg aaactgaact gcacttttgc
    17521 ggcggaactc agcggcgcca ccggtgccac gggctccgta gcggcgcccg cggcttcata
    17581 ggccggcgcc aggagcgtcg gctctggcgc ttgcgtccac ccggccgtgg cccacgccgc
    17641 gcccaacagc gcgcctgccg cgaccctcgc ccacgccccg ccctgcttgt gcttgttcat
    17701 ctggatatgc ctcgctcgct cttgcggatg aagtccacaa aatgctcgat gggcgccgat
    17761 gttgccaccg tcgccacaat tttctccaac gccgccgcca cggtcaggtc ggagcgatac
    17821 acaattttat aggcctcttt cagcgcctgc tgaacttcta gcgggatgcc gtggcgctcc
    17881 aaccccactt tgttcgggcc atagaccttc agcgggtcgc catcggccat catataaggc
    17941 ggtacatcct tcaccacctt ggtgaacccg cccacgatgc tcatccgccc cacccgcacg
    18001 aactggtgca cccccgtcag accgccgatg atggcttcat cctccacgat cacgtgcccc
    18061 gccaacgtgg cgttgttggc catgatgacg cggtcgccca cgacgcagtc gtgcgccaca
    18121 tggcaatagg ccatgatcag gcacttggcg cccacgcgcg tttcaccacc atcaaaggtg
    18181 gcacaattca acgtggcgca ttcgcgaatg acggtttgtt cgcccacgat caggcgcggc
    18241 gcgccgcctt tgtacttcag gtcctgcgtt ttgccgccca cgctggcaaa cggccaaacc
    18301 tcacagcctg cgcccaacgt ggtatgccct tccacactca cgtgcgaacg caacacaacg
    18361 cgatccccca accgcacgtt ctcgcccacc atgcagaacg gaccgacaac cgcatccgcc
    18421 cccaactccg cgttggggtg caccacggcc agcggatgga tgctcacccc catgcctatt
    18481 tgcctccgcc gaacatcatt tcggcctcgc acgccaactc gccatccacc agcgtccggc
    18541 cggctacttt tgccatgccc atgcgtagcc gcagcaattt gatttccatc cgcatctgat
    18601 cccctggcac caccattcgg cggaacttcg cctgatccac gcccagataa tacgacactt
    18661 tccctgccga ccccaccacc gtattcagca ggattcccgc cgtctgcgcc atcgcctcca
    18721 actgcaaaac gcccgggaaa atcggcaacc ccggaaagtg cccctggaag cacggttcat
    18781 tcgccgccac gtttttgatc cccacgatcc aatccttgtt gttgcattcc acaatgcggt
    18841 ccaccagtag gaacggatgc cggtgcggca aaatgttcaa gatatctttg gcttccagaa
    18901 cagtattcat cagacgtctc cttcacaaga atcggggcag cctagcattc tttgcatacc
    18961 gccgacaaat cccaaaacac gccgcggcgg ttcaattgcg cttgccccgc tgtgcccggc
    19021 cacgtatcgt ggccccatcg cgcgaaaggc tcaatatgtc gcaacaccac cgccgctggt
    19081 tcctcaagga agtaccctcc ctgctcgcgg acggcgtcat tgatgccgac accgcccgcc
    19141 gattggaagc gcgctacacc acggccgaac ccggccgcaa ccgggcgctg actgttttca
    19201 gcattttggg cggcaccttg gtaggcctgg gcatcatttt gttgctggcc cataactggg
    19261 cggagctgtc gcgcgcgacc cgcaccgcct tggccttcgc accgctgatc tgcgcgcaag
    19321 ccctcgccgt ctggctgatt cgctccggtc gcgactccgc cgcgtggcgc gaaggcgtcg
    19381 gcctcttctg gacgtttgcc atcggcgccg caattgccct cgtggcgcag acctaccata
    19441 tccccggcga tgccggacgt ttcgccatga cgtggatgct gctcacgctg ccggtgattt
    19501 atctgctgaa tgccagcggg cccgcgctgg tctatctggc cggcatgctc ttctgggccg
    19561 ccgataccca agacaacggt ggcgccgcat gggtcttttg gccgctggcc gccctcctgc
    19621 taccccacac ctatcccgcg gtacggcaaa atccttacgg ctcccgcgcc gcggtgctac
    19681 tctggggcgg tacggtggct ttgtgcattg ccaccgccat tacgctggaa gatttggtga
    19741 gggatcgctg ggtatttgtg tatgccgttc tttttgccgc actctatctg gccggagaaa
    19801 actggctggc cgatgccacc tccaactggc aacgcccact gcgaaccatt ggtggcggtg
    19861 gctgcgtggt gctgaccttc cagctcacct acgactggat gtgggacgat ttcacctggc
    19921 ttttctggac gaatcacctg caaaacaact acgccgccat tctatctaac ctcattctcg
    19981 cgcttatggc tgtggcggtc attgcgttgc tggtgcgtag tgcgcggcac ggcaaaaccc
    20041 acgccctctt catcggcgcg ctgcccatca tggtcggcct cggcaatttc atcgcgcaca
    20101 aattcggcgg cggggccatc tgcctggcga acctctacgc tttcggattg ggcctcgacc
    20161 tccttattcg tggcttccgg acccgccgca atggcaccgt caatttgggg ctgctgctcc
    20221 tctttaccct gattctgttg cggttttggg atgagcgctt cgggtttgtg cttcgcggcg
    20281 tgatcttcgt cgtgctgggc ctcgccttct tgtccgtcaa tctgttcttg gcccgccaca
    20341 aaaaggccac accatgaaaa agtccacgct attgactctg ctggccctcg tcatcggtct
    20401 gcaactggct atccccggcg gcatgatctt gcgccgcgaa tacgtgctga gccacggccg
    20461 cgccttcaag ttccgcgtgg gccccgtgga cccccacgac cccttccggg gccgctacgt
    20521 cgccttgcgc tttgcccaaa actccgtagc aatacccccc ggcctcacca ttcgccgcaa
    20581 ccagaccgtg catgtcttgc tgggcgaaga cgacaacggc ttcgccatct tcaccggcct
    20641 gcaactcacc cggcccaaat ccgatcccta cctgaccacc cgcgtgaatt cggcttatag
    20701 cggcaatgcc attttgcgcc tgccctttga ccgcttctac atggatgaaa aatccgcgcc
    20761 cgcggcggaa atcgttgccc gcaactcgct ggccgacacc aaccggccca cctacgcgga
    20821 agtccgcatc agccgcggct tcgccgtcct cgccgacctc tacgtcgccg accgccccat
    20881 ccgcgaagtc ctcgccgccc cataggattt tgcgctgttt cacacggtgc aagtgcattt
    20941 ggtaagcaaa atactgcgct tgtcccagcc ttctgtatac accataattc gctcacgttt
    21001 ggcgctgggt gttcagcatc cgtagaaagg aaagattatg gccattcctg attatcaatc
    21061 gtgcatgctc ccgctcttac gagtttttgc cgataagcgt gaacatgctt tccgggatac
    21121 tgtggaagcg ctggctcgtg aatttgatct ttccgaagat gagcgacgcg aaatgctgcc
    21181 cagcgggaac caagacgtat tcaccaaccg tgtcggctgg gcacgcacat acttgaaaaa
    21241 agccggcctg ctggaatcca cccggcgtgg cttcaataga attacccctc gcggactatc
    21301 agtgctcgca gaaaaaccaa gccgtattga tgtggcttat ttgggccaat tctcggaatt
    21361 tcaggctttt tgagccttac ggcacacgaa agccgaaggt acactcgagc ttgagtcgaa
    21421 cgccgaaacg ccagaagagg cattggaagg tgcctatctc aaattgcgcg cggatgtcgc
    21481 cgccgatttg ctccaacggc tgacgacatg ttcgccacaa ttttttgagc gattggtagt
    21541 cgaggttatt gtcaagatgg gttatggcgg cacacgccaa gatgccggca aagtgcttgg
    21601 caaaccgggc gacggcggta ttgacggtat tatcaaggaa gacaaattgg gattggatgc
    21661 gattgatatc caagccaagc gctgggaaag cacagttggt cggcccgaaa tacaaagatt
    21721 tgttggcgcg ctgaccgggc atcgagcgaa aaaaggcctt ttcatcacca cctcaaactt
    21781 ttcagccgaa gcccaagcat atgtcacgaa tattgatgcc aaagtcgttt tgattgatgg
    21841 cgagacattg gcgcaactca tgctggatca caatgtcggg gtttcgacta tcggcaccta
    21901 cgaagtcaaa aaggtggatt cagattattt ttcagaagag taaggcttca attattcgcc
    21961 attccctgat gaattgacct atttggggat aaaattgcag caagcaaaag gcccgggggg
    22021 tacccgggcc ttttgagtta cgcggcgcgt gaagcgcgcg tgatttagcc ttccgtctgg
    22081 gtggtcggcg tggtgggcaa ctggtcgccc agcagctctt ccaccgcgat cggctcggcc
    22141 aacggcacca gcttgatgtt gcgatacatc gagaagcccg taccggcggg gatgacgtgg
    22201 cccatgatca cgttttcctt gaacccgtgc aggtgatcca cgcgcgcgag ggtggcggca
    22261 tcggtcagca cgcgggtcgt gtcctggaac gaagcggcgg agatgaagct ttccgtttcc
    22321 agcgaggcct tggtgatgcc caacagcagc ggggaggcct cggcgggctt gcgcccttcc
    22381 tcggccacgc ggcggttgac ctcgttgaag gtttgctttt cgacctgctc gccccagagg
    22441 aagcccgtat cgccgggttc cgaaatccgg accttgcgca gcatttggcg gacgatgatt
    22501 tcaatgtgct tgtcattgat ttccacgccc tgcaaccggt agacctcctg cacttcgttc
    22561 acgaggtatt cctgcagttc ctgcggaccg caaacttcga ggatttcctg cggcactacg
    22621 gggccttcgg tcagcggctg gcccttgcgg acatgatcgc cggcgtacac cacgatgtgc
    22681 ttgttcagcg ggatcaggtg ttcttcggta tcgccggtga tcttgtcgcg gacgagtagt
    22741 tgccggcggc cacgcacctt gccgcccagc tcgacaatgc cgtcaatctt ggcgatgtca
    22801 gcggcatcct tcggcttgcg ggcctcgaac agttcggaca cgcgcggcag accgccggtg
    22861 atgtccttgg tgcgcgcctg cttgcgcggg gtcttggcca gcacggcgcc ggcgtaaacc
    22921 acgtcgccct tttcgacatc cacgcgcgcg ttggcgggca tggtgtaggc ggcgagcacc
    22981 ttcttggggt cgtccaaatc gcggatgatc acctgcggat gaatttcgtc gcggtgttcc
    23041 agaatgacca agtcttccag gccggtggct tcatcctttt ccttacggac ggtgacgccc
    23101 atgatgaagt cgcgcagctc cactttgccg gcgtattcgc tcaagatcgg cacgttgtac
    23161 ggatcccact tggcaatggg gacgttcttc ttgaccgatt cgccatcggc cacggtcagc
    23221 acggagccca acggcaccga gtggcgttcc agctcgcgcc catcttcgtc atacagcacg
    23281 atcataccgg tcttgttcag cacgatgtgg ttgttgtccg gaccgaccac ggtgtacagc
    23341 tcgtcatact tcaagacgcc gtcgcgcttc agcacgattt ccggctgttt gaaggtgctg
    23401 ctggccgtgc caccgatgtg gaaggtccgc atggtcaact gcgtgccggg ttcgccgatg
    23461 gattgcgcgg cgatgatgcc ggtggcctcg cccagcacgg ccgggcggcc gttggccagg
    23521 ttgcggccgt agcacttggc gcaaataccg cgcttggatt cgcacgtcag cacgctgcgg
    23581 atgcgcacgc tgtccagctc ggcgcggcgg atgcgttcgg ccacgcgttc gtcaatttcg
    23641 ttgccggcgg caatgatcat ctcgttcgag cgcgggtcgc ggatgtcgtc gagcgcggtg
    23701 cggcccagga tgcgctgggg cagcggcacg agttcttcat cgccctgcat gatggcgtgc
    23761 acttcaatgc cattgagcgt gccgcaatct tcctcgacca cgatgacatc ctgcgccacg
    23821 tccaccagct tgcgggtcat gtagcccgag tcggcggtct tcagcgcggt gtcggccaag
    23881 cccttgcggg cgccgtgtgt cgaaatgaag tattcgagca cgctcaaccc ctcgcggaag
    23941 ttggccaaaa tgggccgctc gatgatttcg ccggatggct tggccatcag gccgcgcatg
    24001 cccgccaact ggcggatctg ctgccggttg ccgcgggcgc cggagtccac catcatgaac
    24061 agcgggttca catagttgcg cgactcattg aactccatgg cccggaacat ttgcccggtg
    24121 atttcgtccg aggcgtgggt ccagatgtca atgatcttgt tgtagcgctc gccgtcggtg
    24181 atgacaccct tgcggtattg cgcatccact tcagccagcg acttgcgcgc ggcctcgatg
    24241 gtgcgggcct tggcttcggg cacaatcatg tccacgaggc cgatggaaat acccgccagc
    24301 gtggcgtgtt caaagccgag gtccttcacg cggtccaacg tgcggacggt ttcttcgtga
    24361 ccggccacct ggtagcagtg gcggatcagt tcctcgagtt tcttcttggg cacgggggcg
    24421 ttaaagaagc cgaggttgtc cggccagatt tcgttgaaga acacgcggcc caccgtggag
    24481 gtgatgaccg actgatcctt cacgccgtac ttggtttcgc gctgatagtc cggattcttg
    24541 aaacgaatgc ggtcgtgcac ct
//
LOCUS       DAOPNP010000024        24402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2592_length_24402_cov_8.545242, whole genome shotgun sequence.
ACCESSION   DAOPNP010000024 DAOPNP010000000
VERSION     DAOPNP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24402
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2592_length_24402_cov_8.545242"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..3934)
                     /locus_tag="PKI64_03595"
     CDS             complement(350..3934)
                     /locus_tag="PKI64_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOE00127.1"
                     /translation="MKIRGWLVVWAGLVAGSLAWGQSARSGIGAIPYADAGGTGVTFR
                     TWAPNATSVGVRGSFNSWGTTALVKDTPGGNWNGYWSVDVPKAKAGDQYKFKINSTDK
                     KDPRALRQVNSAGNSIVYDQKAFDWGGAANFNAIWRNDLVIYEMHVGTFNAESWLPST
                     FDQCIEKIPHLKKLGVSAVQLMPVMEFPGDRSWGYNASDPYAVESSFGGPDGLKRFVK
                     ACHENGIAVLMDVVHNHYGPSDLDLWQYDGWSQNGKGGIYFYNDWRADTDWGATRPDY
                     GRAEVRDYIHGQIKMLVEDFKIDGFRWDSVYNIIYAGGSDNAQGIQTLNEVNTWLANN
                     HPDVFRIAEDNAFDRTVNFEAEWDRYHFLTDIRGMMTTGSDSARDMSALAYHLSNGGF
                     NRVIYMDSHDTCGDLNNKHRLAYDIDSSNADSYWAKKRALLGNMIALVSPGIPMIFAG
                     SEMHEWYTFSNDQSLRWGRTNTYAGIVQAYSDLIHLRRNLHGVTAGLKQVGKANVHHV
                     NNADKVVGVVRWDQGGQTDDLVLVINASATARTGYSMPFPSAGTWYCLFNSDQTAYDA
                     SFGGVGPAVNGTITASGGTPAANLNLGAYSLQIYSKTPLPQDSAASFDPPNPSGCGST
                     LRITYSSGDGALSNAASVYAYIGRNNWQNPSNHPMVKSNGNWVLNYEIPELTFEVNLS
                     FTDGGSKWDNNDGLNWSVPVANCGDLPATVNWTPQAPNGCVPVTITYKPNGGPLMNAT
                     SIYFHVGHNGWRDVGEQALVKYADNDWRLTYAIPEDTWQLDFVFKDGVGDQGRVWDNN
                     DGKDWHVTVQNCLRLDEPFITITNPAPLTAVSNAVTNIQLRGAAGFLTGRLTWSNQLN
                     SLTGSVAYATNWAISSVPLAEGVNVIRVTGTNSSVNLNNGAWDSPTNSIYTALAPTWT
                     SGANGGQKFKAWEITGDGSGFLAVSNPYCQTGPYAWGLRAQDGKFVQAIRPFSAPLVP
                     GDKLTFFFENGGVDGGLGNSSVGFSFENRFNQRLTQVLFDGGTTNYVIYGSERINTGI
                     PWGNIPRSCIFELLTEQTYQITIGDQQFTGEFAEQSEQLVSRIRFWNWNAGSTDERAV
                     FIGALSITGAPLPVVTYAAEVAVTRTPTALREATSLVVSNGQIIVTVNQANGLHNNIW
                     WADTLLTNRWNWSLLPMEEYQINGQTITFTPAPAVLFRLYSFGKPQGQ"
     gene            complement(4640..5050)
                     /locus_tag="PKI64_03600"
     CDS             complement(4640..5050)
                     /locus_tag="PKI64_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00128.1"
                     /translation="MPDFSTAWKNTLKVVPLCGKSAESFSIAWNFWASFFHSMERIYP
                     IFPRCGKSYETQAAHRVRLSYCIVIGLNLQFGRDYKNQSANSPACRATARTAWKLSLQ
                     KAETADDAKVQTVAGGGDPGSGLNEPSYKNSQQP"
     gene            complement(5233..7257)
                     /locus_tag="PKI64_03605"
     CDS             complement(5233..7257)
                     /locus_tag="PKI64_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE00129.1"
                     /translation="MRPVASSFYFWAMVLAAWCGGWNMPAPAQGPTGGALPDPAPKGA
                     TVLGEGTTRHVVFRTWAPNATRVAVSGDFNGWKEHPMQRDPTLPGYWVLDSRSPKPDQ
                     GYRFIIDGLRRRDPRARAVDMRENKAIILDPRAYPWGPALSWKMPAKDDLVMYEMHVD
                     TFARDIPGSSPPFERAIKRLPYLRALGINCIQLMPVNEFDGERSWGYNPSDIFAIENA
                     YGGANGFKDFIKACHTNGIAVVLDIVHNHYGPSNIACWQFDQSGRADDGGIYFYTDPD
                     RRSTEWGPRPDFSKPEVRDFIVDSLKMFLQEYRLDGFRWDSVHNIRYYLGGAHSNPHG
                     DQLLAEANEWMRKNRPDALRIAEDHAFDGGGVGFDAQWNSAFQATLSAFVRAADSQRD
                     LLMFAEELKQLNGWQWVNFAECHDSAGDLNHHHRLPAYIDPATPDSPRARALALLAGG
                     IVLTVPGYPMLLQGFEMHDVKDFSDLTALPWARAQNTHAGFVRANADLIHLRRNLKGL
                     TPGLKGQALTILHCDNDAKVLAYSRAAAGAPKDNATVVVLNLSGAPCKQYGVRFPTSG
                     TWYCHYNSGSALYAKDFDNIGPKTTEGFILPAGQTTLPLDMSRHSLLIFSKTAPPNVT
                     ATTRAAPAAEIQPPTPEPETQPTREIEEFIEELIPPFPYMPVPLPIEWHP"
     gene            7523..7981
                     /locus_tag="PKI64_03610"
     CDS             7523..7981
                     /locus_tag="PKI64_03610"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOE00130.1"
                     /translation="MKRMAVWMMAGGLMVLAACAGPVEHQQLAERLVDALRVGPLCTE
                     SLHAIVGEAVAVEPRLAGKESELLTFLEEHVGWETLKPAAVALYKARFSEEDLREIVR
                     FYESDAGRRFMEGTMDVAGDIMDLVDRRVKAVSDDWEVLVAELLFQADAQ"
     gene            8000..13303
                     /locus_tag="PKI64_03615"
     CDS             8000..13303
                     /locus_tag="PKI64_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00131.1"
                     /translation="MTRWLDQRWRGAVWGARRLAPGMLGVLVWFGLAGEAAAQKTNRV
                     AITKPPPQYTEDIDTYRARYRYESDRLAAEYDTKASKYRRADGTVDTSDPGWQKLNNK
                     YKQEAAKLRNEYNSQHPSQTSINEVRKTPAGQSLEKTGSDVSSPFSDGDWTAGSDADA
                     AGVVEQLRQKGHRITYDEKLGMYHDETADTKIWEPENPSRSKARASNPEGYTRAGSLE
                     HEGIKSENAQYDPRGYVEDLYKKYEAAKAKGDTRTMNKVAAKMQEATGQRFDPRTQTM
                     RVDGNPLEAGEATLGESPEIRGRKAQAAQQRLDQVVENARTRAEIQSESLTHQRTDLA
                     KQEQGHGKLKSAEEYLEANRKIQRTEPKAGEPAKADAKAPKTEPKPGTKPDGKVPKTM
                     AESAPKGETWRNVLTGKELPVGTSGNQYVDKTAKALSNSKVQAGLQAVGAVMVAKDGY
                     DTVNAVNEAAERGDLDEANKIGSKGVGRLTGAWIGAEAGGKLGAAVGAGLGDGPGGIV
                     GGFLGALGGGILGGMVGDAVGEEVGPGAINKVRDAAVDMATRDVEGAPPGSAEWMRSK
                     GIAPEKVEEFSRRLAAVATSDTAEDNAKKIWQEIKDEVKQARATAVASDGTADGGAGA
                     SAGGDVDGGGGSGGAVDAGVGAGGVIAGAGGSGASVRGGGISTGGGGGAGWGVSGGVA
                     VGGGVPGGGGGGAGIGVGGTRGGADGAAGGDESAATEATASGADEGKAGVDGKDGGGK
                     SKTDKTTDKEHKGDSDGECTAPCDGGDSDPGSTGAAGATSGTAAGGDEGADAGETSAG
                     EESAEADSPPELPVAPPGGEIVDKGWIKGSDGTTTHVVETVDADGNVTKVSHVTYGPD
                     GKVVDVQNYPAGSPTDNADTEVADGQEEIPDDTAADEVEADDKEADDEEADDEEADGT
                     TEDDEWPIEVTLHCHRAASSASGAVEVHSGEYVSVTPGVKGGRPPYLFEWELPHNIVE
                     PDLPFDGNPERFTLESDKSYALLYKPKEGDYTWEGYFTMTARSSGNPANVASDKIKVI
                     VDGRRIELTLHCSRNADPKSGYVIIRKGETVTVSASVKNGRPPYRIEWRLPYNEGGGD
                     ISGNFDELSTVKYKPVGFAWIKNWGFNAIQACVWSSGNTEEWRDPSIGCDTISTFLEW
                     DESDEQSRSITPPPRPVPVPVAPPSVTLPKVTPPSTPPTIPTITGGAPPPPPPAEKEK
                     TPTKPAEPKQIEKKPEVEKPTPPKPSEEIAEPEEPPPPEELEPPEEEEEEPAEETPEC
                     SLSITGHGTSDGRNVFIGRTRDGNRVSISVVGDSQSASAEGVGEASVSLPYVAGDYQI
                     TVQDLDQPECVERQTINIPEPEAEPEPERDCHECLSIGGSTQQSASSLSLADGTSEST
                     MSVSQTYFVEGCPGDRVRISVKGSDGWTASAEAVDSPASVTRTFTTESGTDTITVENL
                     SIPNCSLTFERSFGSSAADLREAEAAAGEGGAVAKTRVVQDVVGSKNARADQRTGNSL
                     QGMAVQQEMQRAATSGDQRVREAQIAVDSAGRAAQATHEETQRRIKAEDTQAAQIMST
                     AIMEGIGTGLAAGGSRLGSGIGEHAAGKIFDHKSKQDKPAPSATAAGSAAPTESAPGK
                     AKSQPKTKPAASEKGDSGKPQTKSTPQPTSAPASGEPTILVDALCPICGEMYNPNQPH
                     QCSGAPKPPPTQALACEICGKPPATVVTTVDSGRKVMCNACQANHRCPRCGKVAMELT
                     GAGYSMGYILPDGTKTNRWASISGVCSQCLAKWKQDQGISP"
     gene            13336..13944
                     /locus_tag="PKI64_03620"
     CDS             13336..13944
                     /locus_tag="PKI64_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00132.1"
                     /translation="MKFNKTTLGILLGALLLAGCETMRPAKTTAPPDKTPTPEERAAM
                     QTQTFPAREGVVFAATIAVLQDLEWKLDKVDRAAGLIQASNPRRLEPLGPREETSTNF
                     EWRRAAIKRRTSEKSQWTRWDSMVIHLEKWPQRQTRCRAILSRNGTLPAMSYPAKVNK
                     QDVIINAPAKEESVELLLPEVYESFFKRLEAAVLDRTGVDKE"
     gene            13954..14541
                     /locus_tag="PKI64_03625"
     CDS             13954..14541
                     /locus_tag="PKI64_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOE00133.1"
                     /translation="MKSRILRNVLLVGLLSVLVAGCASPTGKYFKDVTTPGNVYVLPG
                     HGKIRKIAVMPFRAPTELVGSSVSDMMVTELLRCERYTLVERGQMAQVLNESELALAG
                     LSATKAVEIGTMLGADGVVIGTVDEYGTVAQRGRSYPVVGVSVRLIDCSSGRIVWSVD
                     MAQRAARPDVTLAQHARKVVHDLAGTLYNELRRTR"
     gene            14557..15480
                     /locus_tag="PKI64_03630"
     CDS             14557..15480
                     /locus_tag="PKI64_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00134.1"
                     /translation="MKHRLPVFLLAALVFGGLTACDTTQAPPYHPPSRGSMAAPAPAK
                     TGAPRARPSAQMPRVMILVDEQSLGTIPTAEVEAMAIRMLLDQGVQVVDQDMTQSNLA
                     KGQELFRMAGDNRGAAALGLQFGADVILIGEVVAKPSARRISDSNLRSYQAVATLRAV
                     RTDNSVTMASASEDATVIGLEDVVGSAKAIRAAGQKSLEQIIPAMLQSWVRSGGKSTP
                     QLLHVTLHVGGVDQLWKLRAIREQLKGMPREATDIVQRSYTTGAAVFELDSLVAIEEL
                     AEAIVLHPPADIQFQVLDIGASSIHLKIVSP"
     gene            15508..16467
                     /locus_tag="PKI64_03635"
     CDS             15508..16467
                     /locus_tag="PKI64_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00135.1"
                     /translation="MKKVCRPLVLLLALAGLLLGPQPLIADTPDPAAVLTVNYLRYLL
                     FNHVQAQGQQLVDGAPAMHQPGLQHQLDQWLDAYQETIREDLEAHFPEDARERFEDHI
                     QRFTQAEQDADPAYLAELGHAVGWPGDPAEGYGAFRRWGIEQWVADDMQAGVTFLATL
                     SGTIENLAAAAAPEASPAPARPVDPLRAAEAAPAPLTTGDSAAGAPLQQFSTLREQRR
                     QKALEDAHAGMAQIASERDAWEQEYAANKEAKAQAEAQALQAQAERLAATDQEALEQR
                     KNSFRSRAVNVLAGVASATVSGFTGAIAGRAADEAVHAIFDRK"
     gene            16537..16848
                     /locus_tag="PKI64_03640"
     CDS             16537..16848
                     /locus_tag="PKI64_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00136.1"
                     /translation="MELLPRIFPQCGKNDPKLFHCLEKSLKRCRCARYGSLARPATPV
                     AIELAFAQRPSGSGSALAYFPLCGKKSALRPIFFHGVENYFPHRGKMVGRAPGGLTTE
                     K"
     gene            16900..18156
                     /locus_tag="PKI64_03645"
     CDS             16900..18156
                     /locus_tag="PKI64_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOE00137.1"
                     /translation="MWILRGLLGIAVLVALCWVVSENRRRVQWRVVAWGLALQFLFAL
                     LLVKVPVCQQILAQLNRGVRVIERASHEGGQFVFGYLTGGAAPFLTADGSGAAPALFF
                     FGPLMMLIVVGALSAVLFHFGILQKVVAAFARLLRKTMGLGGAEGVVVAANVFLGQSD
                     APLLIKPYVGHLGRAGLMCVMTAGMATISGTLMVVYAAILEPVLPGIILGHLVVASII
                     SAPAAVMVARVMVPANDADTDHEIPDLKAETHGVVDAVLSGANDGAKVMVNVAIMLVA
                     IIALVALVNKLLGLVPLATPLSLERMAGWVMAPAMWLVGVPWAEAQAAGELMALKTVF
                     NEFVAYLALAKLPPDTLTVHSRLITIYSLCGFANFSSIGILAGTLTALCPEHKATVAS
                     LGLKAMVAGTIATLLTGAVIGILIPP"
     gene            complement(18187..18399)
                     /locus_tag="PKI64_03650"
     CDS             complement(18187..18399)
                     /locus_tag="PKI64_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00138.1"
                     /translation="MGILRILQKLGILRFGATAATYKNAKERPIELQMDGVFNAEKDL
                     INPSDRGGAKPKDEPPAKTPPPSAPV"
     gene            complement(18518..20236)
                     /locus_tag="PKI64_03655"
     CDS             complement(18518..20236)
                     /locus_tag="PKI64_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE00139.1"
                     /translation="MPHRPPDATHQPIGADADGFTTLTLGEQLEFWARTTPDHEFIVY
                     PDRNLRWTYREFDHRVNRLAKGLLAIGIGKGDHVGIWAKNVPDWLTFQFACAKTGAVL
                     VTVNTAYKADEMDYVLRQSDMKMLGLIDGFRDVDYLQIINQLVPELKQHPRGHLHSKA
                     YPHLRHVCYVGQEKHRGMYTTAELMLLGDHQPDRLLADARAAITCHDTVNMQYTSGTT
                     GFPKGVMLSHHNILNNGWSIGHRQRFTAADKLCLQVPLFHCFGVTLGVMAILSHGATL
                     VVVEVFDALLGLAAIHQEKCTVLYGVPTMFIAMYTHPMFKMFDMTSLRTGIMAGAVCP
                     IEVMKRVISDMHITEITSVYGLTEASPGITQSHGHDPVALRVSTVGTAFPGVEVAVLD
                     PATNTPLPPGAEGEICCRGYNVMKGYYKMPQETAAVIDSAGWLHSGDLGTCDADGYYR
                     ITGRIKDIIIRGGENISPKEIEDLLLAMPEVMDAQIVAAPDPKYGELPVAFVILNRGQ
                     SLTEEDVRDYVRQHLAAYKVPRYVFFVTEYPLTASGKIQKYKLREQAKELVAQRLAAT
                     ETAAEH"
     gene            complement(20263..20838)
                     /locus_tag="PKI64_03660"
     CDS             complement(20263..20838)
                     /locus_tag="PKI64_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOE00140.1"
                     /translation="MSQEPNLIGPRIRKLREHRELSVETLAQRTGCSVDMIQALENGE
                     LVPSLTPLLRLARGMGVRLGTLLDDQALDGPAIVRKNDANPAVHFSGNDPSRKRSTLA
                     FHPRAPRKAARNMEPFLIDVRPAEGEIVLNEHEGEEFIYVLSGRIEIRYGKESYQLDA
                     GDSIYYESVVPHHVHALDGAAQILAVVYAPA"
     gene            complement(20912..21550)
                     /locus_tag="PKI64_03665"
     CDS             complement(20912..21550)
                     /locus_tag="PKI64_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOE00141.1"
                     /translation="MNVLHVIANPRPAETSVSKKLAFEFFAALMEKNPDVVVNNVDLY
                     QNKPPYVSSEALNYFWGPVVNPAYIPSKAEETAANFSNNNAQALVDADVLVLTMPVWL
                     NSFPAILKAWLDQVLVPGKLFTFGPDGVIPIHHLRTVVLLVSSDSVYKEDDATDGLTP
                     AIRAIFSYIGVDDIQIAWADGQDASRHFDSEQRREMALEAAREIAEDLAEQV"
     gene            21704..22930
                     /locus_tag="PKI64_03670"
     CDS             21704..22930
                     /locus_tag="PKI64_03670"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOE00142.1"
                     /translation="MDIQNLFAERIGGAQFGQSTAIYKFEKIKRAKRAAQAAHPGVEM
                     LDFGVGEPDQMAPKAIRQALKKAVDDPANRGYADNGIAEFKVAAAKYMADFFGVTDLD
                     PATEINHTIGSKPALAMLPLCFVNPGDAVLATIPGYPVLATHTKYLGGTVIPVPLLAK
                     NKFFPDLKALEQTDLSRVKLFYVNYPNNPTGAAATVEFFDELIAFAQKHNILIVQDAA
                     YATLIYGRERLSILSRPGGKEAAIELHSMSKSYNMTGWRLGFVAGAARTVAAVAEVKD
                     NIDSGQFKAIQVAACAGIADQKLSEKIRAHYQRRLRGLVKALKGAGFHARMPGGTFYL
                     YVPAPKGTADGRTFANAEEASQFLITEHCISTVPWDDAGAYLRFGATFESAGRADDAR
                     VLAELARRLQAAQLVF"
     gene            22942..23367
                     /locus_tag="PKI64_03675"
     CDS             22942..23367
                     /locus_tag="PKI64_03675"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOE00143.1"
                     /translation="MSQAKIIFAKGTQLTVYIDNRKGTLAALTTFLAKHGVNIYGLTL
                     ADTEGHGYARLIVDDTEKARQLVEDSGELVAAREVLLIRVANEPGELARMLTTLAAHN
                     LNIEYGYSAGGPGDEKGLVLVPSDVDAALAVLKELNAGH"
     gene            23369..24091
                     /locus_tag="PKI64_03680"
     CDS             23369..24091
                     /locus_tag="PKI64_03680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00144.1"
                     /translation="MPPAPKVSIITSTYNRSAVLRRAIESVRAQRDFDDWEMCVVGDC
                     TPDDSAVVVASFGDPRLRFYNLPEKSPPRAHGAIAKNYGVFQMARAPYIAYLDDDDEY
                     HPDFLRVMMGVVARTPEQPVFYCRCQYRDKKTGRRIWGNPFQPWLHRWSRKKLLRYNF
                     INTANVVHKKSLIDEVGGWDPNDYFDDYDLWKRMSARCDFHYVNRALVISHVGDCPPF
                     FVRLFTKGWKILRYGRRLQDQG"
     gene            complement(24095..>24402)
                     /locus_tag="PKI64_03685"
     CDS             complement(24095..>24402)
                     /locus_tag="PKI64_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00145.1"
                     /translation="KAIRESLATLPPERDIEMVNIGNALAHERRIRLIRQLLQQPLPL
                     GELLMNSRIPIRPFYLHLQTLQAGGFIVILERQVQLITPPHPLAKALIRLIQQGMTC"
BASE COUNT         4850 a         6840 c         8035 g         4677 t
ORIGIN      
        1 gttgatccac atcaccatga tgcatgaacg aaaccccata gcatctacaa tccgaacgga
       61 ttttttgtag ggcgggaatg gttgaggaac gataccttcc cgccgggaac ggctggaagg
      121 tctacgaccg ttccagccct tcaatccgaa cggatttttt gtagggcggg aatggttgag
      181 gaacgaaacc ttcccgccgg gaaaggctgg aaggtctgcg accgttccag cccttcaatc
      241 cgaacggatt ttttgtaggg cgggaatggt tgaggaacga aaccttcccg ccgagagaag
      301 gctggaaggt ctacgaccgt tccagcccaa caagattgat gaagggaccc tattggccct
      361 gcggcttgcc gaaggaatac agccggaaca acacggccgg ggccggcgtg aaggtgatgg
      421 tctgtccgtt gatttgatat tcctccatgg gcagcaacga ccagttccat cggttggtga
      481 gcagggtgtc cgcccaccaa atgttgttgt gcaacccatt cgcctgattg accgtgacga
      541 tgatctgccc gttgctgacc acaagtgacg tggcctcgcg cagggcggtt ggtgtgcggg
      601 tgacggcgac ctcggcggcg taggtcacca ccggcaatgg cgcgccggtg atggaaaggg
      661 cgccaatgaa tacggcgcgt tcgtcggtgc tgcccgcgtt ccagttccag aagcggatgc
      721 gggagacgag ttgttcggac tgctcggcaa attcgccggt gaattgttgg tcgccgatgg
      781 tgatttggta ggtctgttcg gtaaggagtt caaagatgca actgcgcggg atgttacccc
      841 aagggatgcc ggtgttgatg cgttcggagc cgtagatgac gtaattggtg gtgccgccgt
      901 cgaagagcac ctgggtgagc cgctggttga agcgattctc gaaggagaag ccaacggagc
      961 tattgcccaa gccgccgtca acgccaccat tctcaaagaa gaaagtgagt ttgtcgccgg
     1021 ggacaagcgg ggcggagaag gggcggatgg cttggacgaa cttgccgtcc tgagcacgga
     1081 gaccccatgc ataaggaccg gtttgacagt acggattgga gacggcgagg aagccggagc
     1141 cgtcgccggt gatctcccac gccttgaact tctggccgcc gtttgcgccg ctggtccaag
     1201 tgggggcaag ggcggtgtag atggagttgg tgggggaatc ccacgcgcca ttgttgaggt
     1261 tgacggagga gttggtgccg gtgacgcgaa tgacgttgac tccttccgcc agcggcacgg
     1321 acgaaatggc ccagttggtg gcgtaggcca cgctgccggt gaggctgttg agctggttgc
     1381 tccacgtcag gcggccggtc aggaagccgg cggcaccacg gagttggatg ttggtgacgg
     1441 cattgctgac ggccgtaagc ggcgcggggt tggtgatggt gatgaacggc tcatccaagc
     1501 gcaggcagtt ttgtactgtc acgtgccaat ccttgccgtc gttgttgtcc cacacgcgcc
     1561 cttgatcgcc cacgccgtcc ttgaacacga aatcgagttg ccaggtgtct tcgggaatgg
     1621 cgtaggtgag ccgccagtcg ttatcggcat atttgaccaa cgcctgctcg ccgacatcgc
     1681 gccagccgtt gtgccccacg tgaaagtaga tgctggtggc attcatcaga gggccgccgt
     1741 tgggtttgta ggtaatggtg accggcacac agccattggg ggcttgcggc gtccagttga
     1801 ccgtggccgg caggtcgccg caattggcga ccggcacgct ccagttgaga ccatcgttgt
     1861 tgtcccactt gctgccgcca tcggtgaagg agaggttgac ttcaaaggtc agctcgggaa
     1921 tctcatagtt gagcacccaa ttgccgttgg atttgaccat ggggtggttg gagggatttt
     1981 gccagttgtt gcggccgata taggcataga cgctggccgc gttggagagc gcgccatcgc
     2041 cggaactgta ggtgatgcgc agggtggagc cgcacccgct ggggttgggc gggtcgaagc
     2101 tggcggcgga gtcctgtggg aggggggtct tggaatagat ttgcagcgag taggcgccaa
     2161 gattgaggtt ggccgctggc gtgccgccac tggcggtgat cgtgccattg acggcagggc
     2221 caacgccgcc aaaggaggcg tcgtaggcgg tctggtcgga attgaagagg caataccatg
     2281 tgccggcgga ggggaacggc atggagtagc cggtgcgggc agtggccgag gcgttgatga
     2341 ccagcacaag gtcgtcggtc tggccgccct ggtcccagcg gacgacgccg accaccttat
     2401 cggcgttgtt gacgtgatga acgttggctt tgcccacttg cttgaggccg gcggtgacgc
     2461 cgtggagatt gcgccgcagg tgaatgaggt cgctataggc ctgcacgatg ccggcatagg
     2521 tgttggtgcg gccccagcgg agggattggt cattggaaaa ggtgtaccac tcgtgcattt
     2581 cggagccggc aaaaatcatg gggatgccgg gggaaaccag tgcaatcatg ttgccgagca
     2641 gcgcgcgttt tttcgcccag tagctatcgg cattgctcga atcaatatca taggcgaggc
     2701 ggtgtttgtt gttgaggtcc ccgcaggtat cgtggctgtc catgtagatc acgcggttga
     2761 agccgccgtt gctgaggtgg taagcgaggg cgctcatgtc gcgggcggag tcgctgccgg
     2821 tggtcatcat gccgcggatg tcggtcaaga agtggtagcg gtcccactcg gcctcgaagt
     2881 tgacggtgcg gtcaaaggcg ttgtcttcgg cgatgcggaa gacatcgggg tggttgttgg
     2941 cgagccaggt gttgacttcg ttgagggttt gaatcccctg cgcgttgtcg gagccacccg
     3001 cgtagatgat gttatagacc gagtcccagc ggaacccatc aattttgaaa tcttcgacca
     3061 gcattttgat ttggccgtgg atgtagtcgc ggacctcggc gcggccgtaa tcagggcggg
     3121 tggcgcccca atcggtgtcg gcgcgccaat cgttatagaa atagatccca cccttgccgt
     3181 tttgcgacca gccgtcgtac tgccagaggt caaggtcgga ggggccgtag tggttgtgga
     3241 ccacatccat gagtacggcg atgccgtttt cgtggcaggc cttgacgaac cgtttaagac
     3301 cgtcggggcc gccaaacgag gattccacgg cgtaggggtc ggaggcgttg taaccccacg
     3361 aacggtcacc ggggaactcc atgaccggca tgagctggac ggcggaaaca cccaattttt
     3421 tcaggtgggg gattttctcg atgcactggt caaaggtgga cggcaaccag ctttcggcgt
     3481 tgaaggtgcc cacgtgcatt tcataaatca ccagatcatt gcgccagatg gcattgaagt
     3541 tggccgcgcc gccccagtcg aatgccttct ggtcatagac gatggaattg cccgccgagt
     3601 tgacttggcg caaagcacgc gggtcttttt tgtcggtgct gttgattttg aatttatact
     3661 ggtcgcccgc cttggccttg gggacatcca cggaccagta gccattccag ttgccgccgg
     3721 gggtatcttt caccagagcc gtggtgcccc aggagttgaa cgagccgcgc accccgacgg
     3781 atgtagcgtt gggcgcccac gtccggaagg tgacccccgt gccgcccgca tcggcgtacg
     3841 ggatggcgcc gatgccactg cgcgcggatt gcccccacgc cagagaaccc gccaccagtc
     3901 cagcccagac caccaaccaa ccgcgtattt tcataacata gacctaacag actgcgcaga
     3961 tatttgcaaa agctttgcgg gaaataattg tgcggttagc gtgcctgtcc gcagcggggc
     4021 atttcgtcgt ttctgggggc gctggcgcgc tgccgcctcg cggcgcgctc ggcggagagg
     4081 tcagaggtca gatttcatgg agcagccacc gacccaagca ggaaaacaga aaagagcatt
     4141 caacgcaagc gacgccaacg ccacaagcgt cgcaaacaag atttactgta gccgggctcg
     4201 ttgagcccgg ggctgttggt tcgctggtct tcaacttaac ctctgaaccc tgacctctga
     4261 accctccgcc gccttgcggc ggtgcggggg ctgttggcct tcctgtaggc cgccgttgta
     4321 ccggcggcgg gcggttggat tttctgtctt tattgtagcc ggcctcgctg aggccggtgt
     4381 ccggaagaaa atccccgcgg tcaccgcccg cggctacagt taacagccag gcttttaagg
     4441 ctgcggcggg ctgttggctg ctcccctgta acctgcaacc tttaacctgt aaccgtcttg
     4501 ctgtagccgg gctcgttgag cccggggctg tttggctggt tttgctcagc ggttcagagg
     4561 tcgcaaaaaa aacgacggcc ctgttttctt cccatgcctt ttcctgtctt cctgttgatt
     4621 cctgttttcc tgcttggttc tacggctgct ggctgttctt gtaggagggc tcgttgagcc
     4681 cgctgccggg gtcaccgccc ccggctacag tctgaacctt tgcatcgtcg gctgtttccg
     4741 ccttctggag ggacagcttc cacgccgtcc gcgcggtagc gcggcaagcc gggctgttgg
     4801 cgctttggtt tttgtaatcc ctgccgaact gaagattcag cccaattacg atgcagtaag
     4861 ataatcttac ccgatgtgct gcctgcgttt cgtatgattt tccacaccgt ggaaaaatcg
     4921 ggtaaattcg ttccatactg tggaaaaatg aggcccaaaa gttccacgca atggaaaaac
     4981 tttcggcgga ttttccacac aatggaacaa ctttgagggt gtttttccac gctgtggaaa
     5041 aatcgggcat aaaagttcca caccgtggaa aaatcccgcc cacgcgggat aaataatttc
     5101 cacgctatgg aaaaatccgg gggcgaccgc caattgtttt ccacaccgtg gaaaagtgag
     5161 gcgatttgga aaaaggcgag gttttatgaa gggaaaaagc cagccaacat cttatcgagt
     5221 gaatggctca agttaggggt gccactcgat ggggaggggg acgggcatgt atgggaaggg
     5281 ggggatgagt tcttcgatga actcctcgat ctcacgagtg ggctgggttt ccggttcggg
     5341 ggtggggggc tggatttcgg cagccggcgc ggcgcgggtg gtggcggtga cgttcggcgg
     5401 cgcggtcttg gaaaagatga gcagcgaatg gcggctcata tcgagcggca gggtggtttg
     5461 gccggcgggc aggatgaaac cttcggtggt cttggggccg atgttgtcaa aatccttggc
     5521 gtagagcgcg gagccggagt tgtaatggca gtaccacgtg ccggaagtgg gaaaacgcac
     5581 gccgtattgt ttgcacggcg cgccggacaa attgagaacc acgacggtag cgttgtcctt
     5641 tggtgcgccg gcggcggcgc gggaataggc gagcaccttg gcgtcgttgt cgcagtgtag
     5701 gatggtcagg gcttggcctt tgaggccggg ggtgaggccc ttgagattgc ggcgcaggtg
     5761 aatcagatcg gcattggccc gcacgaaccc ggcgtgggtg ttttgtgcac gggcccaggg
     5821 gagcgcggtg aggtcgctga agtctttgac atcgtgcatt tcgaagcctt ggagcagcat
     5881 ggggtagccg ggcacggtca ggacgatgcc gccggccagc agggcgaggg cgcgggcgcg
     5941 cgggctgtcg ggcgtggccg ggtcaatata ggcgggcagg cggtggtgat gattgaggtc
     6001 gcccgccgag tcgtggcatt cggcaaagtt gacccactgc cagccgttga gttgtttgag
     6061 ttcttcggca aacatgagca ggtcgcgctg cgaatccgcg gcgcggacaa aggcggagag
     6121 tgtggcctga aacgcggagt tccactgggc gtcgaagccc acgccaccgc catcgaaggc
     6181 gtggtcttcg gcaatccgca gggcatcggg ccggtttttg cgcatccatt cgttggcctc
     6241 ggccagcaac tggtccccgt ggggattgga gtgcgcgccg cccagatagt agcggatgtt
     6301 gtgcaccgag tcccagcgga agccgtccaa gcggtattcc tgcaggaaca ttttgaggga
     6361 atccacgatg aagtcgcgca cctcgggctt gctgaaatcc gggcgcgggc cccactccgt
     6421 ggagcggcgg tcggggtcgg tataaaaata gatgccgcca tcatcggcgc gcccgctttg
     6481 gtcaaactgc cagcaggcga tgttgctggg gccatagtgg ttgtggacga tgtccagcac
     6541 cacggcgatg ccgttggtgt ggcaggcctt gatgaaatcc ttgaagccgt tggcgccgcc
     6601 gtaggcgttt tcgatggcga aaatatcgct ggggttgtag ccccaggaac gctcgccatc
     6661 gaactcattg accggcatga gttggatgca gttgatgccg agcgcgcgca ggtaggggag
     6721 gcgtttgatg gcgcgttcga agggcgggct gctgcccgga atatcccgcg cgaacgtgtc
     6781 cacgtgcatt tcgtacatca ccaggtcatc cttggccggc attttccacg aaagcgccgg
     6841 gccccacggg taggcgcgcg ggtccagaat gatggccttg ttttcgcgca tgtccacggc
     6901 gcgcgcgcgc ggatcgcgcc gccgcaggcc gtcaatgatg aagcggtagc cctgatcggg
     6961 cttgggcgag cggctgtcca gcacccaata gcccggcagc gtggggtcgc gctgcatggg
     7021 gtgttccttc cagccattga aatcgccgct gacggccacg cgcgtggcgt tgggcgccca
     7081 tgtgcggaat accacgtgac gcgtggtgcc ttcgccgagg acggtggccc ccttgggcgc
     7141 gggatcaggc agcgccccgc ccgtaggtcc ctgcgccgga gccggcatgt tccacccgcc
     7201 gcaccacgcg gccagcacca tggcccagaa atagaatgaa gatgcaacgg gtctcatgcc
     7261 tgtccgaaca gactagcgga gggcgctagc ggtcggcaag cggggaaatc ggaaaatcag
     7321 ggttggccag cggggcggca ttggcgggcg gcgctggtac gtgcgagtag atttctgcgg
     7381 gccggttggg aacaagcgct aacacaattt taacatgata aagagtgctt tatgtaaaca
     7441 ggttgttaga atcaggttgg atcgccttgc tgataccaac agttgggtcg ttggcggggt
     7501 ggcgcggaat tggaggccgg agatgaaacg catggcagtg tggatgatgg cgggagggtt
     7561 gatggtgctg gcggcgtgcg cggggccggt ggagcatcag cagttggcgg agcggctggt
     7621 ggatgcgttg cgggttgggc cgctgtgcac ggagtcgctg catgccatcg tgggcgaggc
     7681 ggtggcggtt gaaccccggc tggcgggaaa ggaaagtgag ttgctgactt tcctcgaaga
     7741 gcatgtgggc tgggaaacct tgaagccggc ggcggtggcc ttgtacaagg cacgtttctc
     7801 ggaggaggac ctgcgcgaga ttgtccggtt ttatgaaagc gatgccgggc ggcgctttat
     7861 ggagggaacg atggatgtgg ctggcgacat catggacctg gtggatcgcc gggtgaaggc
     7921 ggtctcggat gactgggaag tcctggtggc ggagttgtta tttcaggcgg atgcgcagta
     7981 ggcaaagggg aggccggtga tgacaagatg gcttgatcaa aggtggcgtg gcgcggtgtg
     8041 gggtgcgcgc cgattggcgc cggggatgct gggggtgctg gtttggtttg ggctggcagg
     8101 ggaggcggcc gcccaaaaaa cgaaccgggt ggcaattacc aaaccgccgc cacagtacac
     8161 cgaggatatt gatacctacc gcgcgcgcta tcgctatgaa agcgatcggt tggcggcgga
     8221 gtatgacacg aaggccagca aatatcgccg cgccgatggc acggtggata cgtccgaccc
     8281 cggctggcag aagctcaaca acaagtacaa gcaggaagct gccaagctgc gtaatgaata
     8341 caacagccag cacccctcgc agacctcgat aaatgaagtg cgcaaaactc ccgcgggaca
     8401 aagtctggag aaaaccggca gcgatgtctc cagcccgttt tcggacggcg actggaccgc
     8461 cggcagcgat gccgatgccg ccggcgtggt ggagcagctc cgccagaagg ggcatcgcat
     8521 cacctacgat gaaaagctcg ggatgtatca tgatgagacc gccgacacca aaatctggga
     8581 gccggaaaat ccgtcgcgct ccaaggcgcg ggcgtccaac ccggaaggct acacgcgggc
     8641 cggctcgctg gagcatgagg ggatcaagtc ggagaatgcg cagtatgatc cgcgcggcta
     8701 tgtagaagac ctctacaaaa agtacgaagc ggccaaggcc aagggcgaca cgcgcacgat
     8761 gaacaaggtg gcggccaaga tgcaggaggc caccggccaa cgttttgacc cgcgcaccca
     8821 gaccatgcgc gtagatggca atcccctcga ggccggggaa gccacgctgg gcgagtcgcc
     8881 ggagattcgt ggccgcaagg cgcaggccgc gcaacagcgc ctggaccaag tggtggaaaa
     8941 cgcgcggacg cgcgcggaaa tccagtcgga atcactcacg caccagcgga cggatctggc
     9001 caagcaggag caggggcacg gcaaactaaa atcggccgag gaatatctgg aagccaaccg
     9061 aaaaatccaa cgcacggagc cgaaagcggg cgagcccgcc aaggcggatg ccaaagcacc
     9121 gaaaacggaa cccaagccgg gaaccaagcc ggatggcaaa gtgcccaaaa ccatggccga
     9181 gtccgctccc aagggcgaga catggaggaa tgtcctcacc ggaaaagagt tgccggtggg
     9241 cacctcgggc aaccagtacg tggacaagac cgcaaaagcg ctcagcaatt ccaaagtgca
     9301 agccggtttg caggcggttg gcgcggtgat ggtggccaag gatggctatg ataccgtaaa
     9361 cgccgtcaac gaggctgcgg aacggggcga cttggatgaa gccaacaaaa taggttccaa
     9421 gggcgtgggg cggctgacgg gtgcctggat tggcgcggaa gccggcggca aactgggggc
     9481 cgcagtcggt gccgggctgg gcgatggccc cggcgggata gtcggtggtt ttctcggggc
     9541 gctgggtggc ggcatcctcg ggggtatggt aggcgatgcc gtcggcgaag aagtgggccc
     9601 cggcgccatc aataaggtgc gcgatgctgc cgtggatatg gccacgcgcg atgtagaagg
     9661 cgcgcccccg ggtagcgcgg aatggatgcg cagcaagggt attgcgcccg aaaaggtgga
     9721 ggagttttcc cgaaggttgg ccgcggtggc cacgagcgat accgccgaag ataacgccaa
     9781 gaagatttgg caggagatca aagatgaagt caaacaggcg cgcgccacgg cggtcgcaag
     9841 cgatggcaca gcggatggcg gtgccggtgc cagtgccggg ggtgatgtag atggtggtgg
     9901 cggttcaggc ggtgctgtag atgctggggt cggtgcggga ggcgtgatcg cgggggcggg
     9961 gggaagcggt gcttcagtga ggggcggggg aatatccacc ggtgggggtg gcggagcggg
    10021 ttggggggtg tcgggcggcg tggcggtcgg tggtggtgta cccggcggcg gcggaggtgg
    10081 tgctgggatt ggcgtgggcg gcacacgagg gggggcggac ggtgccgcgg gaggcgatga
    10141 atccgcggcg actgaggcta ctgccagcgg cgcggacgaa ggcaaggccg gagtggacgg
    10201 caaagatggc ggcggaaaat ctaagaccga caagaccacg gacaaagaac acaaggggga
    10261 ttcggatggc gagtgcacgg cgccgtgtga tgggggcgac tccgatccgg gctcgacagg
    10321 agcggcaggc gcgacatcgg gaaccgccgc gggtggcgat gagggggctg acgccggtga
    10381 aacttcggca ggcgaagaat cggccgaggc ggattcgccg ccggaattgc cggtggcgcc
    10441 gcccggcgga gaaatcgttg ataaaggatg gatcaagggc agcgacggca ccacgaccca
    10501 cgtggtcgaa accgtggatg ccgatggcaa cgtgaccaag gtcagccatg tcacctatgg
    10561 ccccgacggc aaggtggtgg atgttcagaa ctatccggcg ggctcaccca cggataacgc
    10621 ggacacggaa gtcgctgatg ggcaggagga aataccggat gacaccgcgg cggacgaggt
    10681 agaggcggat gacaaagagg cggacgacga agaggcggac gacgaagagg cggacggaac
    10741 aaccgaggat gatgaatggc cgatcgaggt aactctgcat tgccatcgcg ccgcctcgtc
    10801 tgcatcaggt gccgtggaag ttcactcagg tgaatatgtg agcgtgactc cgggcgttaa
    10861 ggggggccgt cccccgtacc tgtttgaatg ggagttgccg cacaatatag ttgagccaga
    10921 tctgccgttc gatggaaacc cggagcggtt caccttggaa agcgataaat catacgctct
    10981 tttgtataag ccgaaggagg gggactacac ttgggaaggc tatttcacga tgactgcgcg
    11041 gagttcggga aacccagcca acgtcgcgtc cgataaaatc aaagttatcg tggacggaag
    11101 gcgaatcgag ttgactctgc attgtagccg caatgcagat ccgaaaagtg gttatgtgat
    11161 aattcgcaag ggggaaaccg taaccgtaag cgcatccgtc aagaatggcc gtcccccgta
    11221 tcggattgag tggcggttgc cgtacaatga aggggggggg gacatcagcg ggaattttga
    11281 tgaattgagc acggtcaaat acaagcctgt ggggtttgct tggattaaaa attggggatt
    11341 taacgcaatc caagcatgtg tatggagttc gggaaacaca gaagagtggc gtgaccctag
    11401 catcggctgc gatacgatct ctactttctt ggaatgggat gagtcggatg aacaaagtcg
    11461 gtcgataacg ccaccgccga gaccggtccc agttccggtt gctccgccat cggtgacttt
    11521 gcccaaggtg actcctcctt ccactccgcc gaccattccg accatcaccg gcggcgcacc
    11581 gcccccgccc ccgccggcgg aaaaggaaaa aacgcccacc aaacccgctg aacccaagca
    11641 gattgagaaa aagccggagg tagaaaagcc cacgcctccg aaaccatctg aggagatcgc
    11701 ggaaccggaa gaaccgccgc cgccggaaga gctggagcca ccagaggaag aggaggaaga
    11761 gccggcggag gaaacgcccg agtgctcgct ctccatcacc gggcatggca cctctgacgg
    11821 acgcaatgtg ttcatcggcc gcacccgcga cggcaaccgg gtctcgatct ccgtcgtggg
    11881 cgattctcaa tccgcttctg ccgaaggcgt cggcgaagcc tccgtctccc ttccttatgt
    11941 ggccggcgat tatcaaatca cggttcagga cctcgaccaa cctgagtgcg tcgaacgcca
    12001 gaccatcaac atccccgaac ccgaagcgga acccgaaccc gaacgcgatt gccacgaatg
    12061 cctctccatt ggcggctcca cgcagcagtc ggccagctcg ctttcgctcg ccgatggtac
    12121 ctccgaaagt actatgagcg tcagccaaac ctacttcgtt gaaggctgcc ccggcgaccg
    12181 cgtgcgcatc agcgtgaaag gctccgatgg ctggacggct tccgccgagg cggtggactc
    12241 tcccgccagc gttacccgca ccttcaccac cgaaagcggc actgacacca tcaccgtgga
    12301 aaacctgagc atccccaact gttcgctcac ctttgagcgc agtttcggtt cgtcggccgc
    12361 cgacttgcgc gaagcggaag ccgccgcggg cgaggggggg gcggtggcca aaacgcgcgt
    12421 ggtgcaggat gtggtgggca gcaagaacgc gcgcgccgat cagcgcaccg gcaacagcct
    12481 gcagggcatg gcagtgcaac aggagatgca gcgggccgca acctcgggcg accaacgcgt
    12541 gcgcgaggcc cagatcgccg tggatagcgc cgggcgcgcg gcgcaggcca cccacgagga
    12601 gacccagcgt cggatcaaag ccgaagacac ccaagccgcc caaatcatgt ccaccgcaat
    12661 catggagggt atcggcaccg ggttggcggc cggtgggtcg cgcttgggct cgggcattgg
    12721 cgagcatgct gccggcaaga tctttgacca caagagcaaa caggacaaac ccgcgccgtc
    12781 ggccacggcg gcaggcagcg ccgcgccgac cgagagtgcg ccgggcaagg ccaagtccca
    12841 gcccaaaacc aagcctgccg ccagtgaaaa aggcgattcc ggcaaaccgc aaaccaagtc
    12901 cacgccgcaa cccacatcgg cccccgccag cggtgagccg acaatactgg tggacgcgct
    12961 ctgcccgatc tgtggcgaga tgtataaccc gaatcaaccg caccagtgtt cgggtgcgcc
    13021 caaaccaccg ccgacgcaag cgctcgcttg cgagatttgc ggcaagccgc cggcgacagt
    13081 agttaccacg gtggatagtg gccgcaaggt catgtgcaac gcttgtcagg ccaaccaccg
    13141 ctgcccccgt tgcggcaaag tggcgatgga attgaccggc gccggctaca gcatgggcta
    13201 catcctgccc gacggcacaa aaaccaaccg ctgggccagt atcagcgggg tttgcagcca
    13261 atgtttggcc aagtggaaac aagatcaagg aatatcccca taacccatgg ccgcccccag
    13321 aaaaggagat cacgcatgaa attcaacaaa accactctcg gcatcctcct cggtgcgcta
    13381 ctgctggccg gctgcgaaac gatgcgtccg gccaaaacca ctgcgccacc ggataaaaca
    13441 cccacgccgg aagaacgcgc ggccatgcaa acacaaacat tccctgcgag ggagggcgtc
    13501 gtctttgccg caacaatcgc ggtgctgcaa gacctcgaat ggaagctgga caaagtggat
    13561 cgcgcggcag gcttgattca ggcctccaat ccgcggcgct tggagccgct gggcccgcgc
    13621 gaggaaacca gcaccaactt cgagtggcgg cgtgcggcca tcaagcggcg cacttccgaa
    13681 aaaagccagt ggacccggtg ggactccatg gtgattcatc tggaaaaatg gccgcagcgg
    13741 caaacccgct gtcgcgccat tctttcgcgt aacggcacgc tgccggcgat gtcctacccg
    13801 gccaaggtga acaagcagga tgtgatcatc aacgcgccgg ccaaggagga atccgtggag
    13861 ctactgttgc cggaagtata tgaaagtttt ttcaagcggt tggaagccgc cgtgctggat
    13921 cgcacgggcg tggataagga gtaggaggag ccgatgaaat caagaatcct gcgcaacgtt
    13981 ctgctggttg gtttgttgtc ggtgctggtg gcgggctgtg cgtcgcccac cggcaaatac
    14041 ttcaaggatg tcactactcc cggcaatgtc tatgtcctgc ccggccacgg caaaatccgt
    14101 aaaattgccg tcatgccgtt ccgcgcgccc acggaactgg tgggcagctc ggtgtccgac
    14161 atgatggtca cggagctgtt gcgctgcgag cgctataccc ttgtcgagcg cggccaaatg
    14221 gcgcaggtgc tcaacgaatc cgaattggcc cttgccggtc tctccgccac caaggccgtg
    14281 gaaatcggca ccatgttggg cgcggatggc gtggtgatcg gcacggtgga tgaatatggc
    14341 accgtggctc aacgcggccg ttcctatccc gtggtgggtg tttcggtgcg cctgattgac
    14401 tgctccagcg gacggatcgt ttggagtgtg gacatggcgc agcgtgccgc ccgcccggat
    14461 gtcacgctgg ctcagcatgc ccgcaaagtg gtgcatgatc ttgccggcac cctatacaac
    14521 gaattgcgcc gtacccgcta atccatggag tccgctatga aacaccgcct tcccgttttc
    14581 ttgctggctg cgctggtctt tggtggcctc accgcgtgcg ataccaccca agcgccgccg
    14641 tatcacccgc cgtcgcgcgg cagcatggcg gcgccagcgc cggccaaaac cggcgccccg
    14701 cgcgcccgcc cctccgccca aatgccgcgc gtgatgattc tggtggacga gcaaagtctg
    14761 ggcaccatcc ccaccgccga agtcgaagcc atggccatcc gcatgctgct ggaccaaggg
    14821 gtgcaggtgg tggatcagga tatgacccag tccaacctcg ccaagggaca ggaactgttc
    14881 cggatggccg gcgataatcg cggggcggcc gccctcggcc tccagttcgg cgccgatgtc
    14941 atcctcattg gcgaagtcgt ggccaagcct tccgcgcggc gcatcagcga ctccaacctg
    15001 cgctcctatc aggccgtcgc cacgctacgc gcggtgcgca cggataattc tgtcaccatg
    15061 gcgtctgcct cggaagatgc cacggtgatt ggcctcgaag acgtggttgg cagcgccaag
    15121 gccatccgcg ccgccgggca aaaatccctt gaacaaatca tccctgccat gctccagtcg
    15181 tgggtccgtt ccggtggcaa atccacgcca caactgctcc acgtcacgct gcatgtcggc
    15241 ggcgtggatc agctctggaa gctgcgcgcc atccgtgagc agctcaaagg aatgccccgc
    15301 gaggccaccg acattgtcca acgttcctac accaccggcg cggcggtctt cgagctggat
    15361 tcacttgtcg ccattgagga gttggccgaa gccatcgtcc tccatccgcc tgccgacatc
    15421 caattccaag tcctcgatat cggcgccagc agcattcacc tcaaaattgt gagtccctaa
    15481 ctgatcggca acccggagag tctcgccatg aaaaaagtct gccgccccct cgtgctcctg
    15541 cttgctcttg cgggactcct gcttggccca cagccgctga ttgcggacac gcctgatccc
    15601 gccgctgtcc tgaccgtcaa ctacctgcgc tatctgctct tcaaccacgt ccaagcccag
    15661 ggccaacagt tggtggatgg cgccccggcc atgcaccagc ccggcctcca gcatcagctc
    15721 gatcagtggt tggacgctta tcaggaaacc attcgcgagg atttggaggc gcactttcct
    15781 gaagatgccc gcgaacggtt tgaagaccat atccagcgct tcacccaagc cgagcaggat
    15841 gccgatccag cctacctcgc cgagttgggc cacgcggtcg gctggcccgg cgatcccgcc
    15901 gaaggctatg gcgccttccg ccgctggggc attgagcaat gggtggctga tgacatgcaa
    15961 gccggcgtca cctttctggc caccctcagc ggcaccattg agaatttggc ggccgccgcc
    16021 gcaccggagg cctcgcccgc gccggcccgc cctgttgatc ccctgcgcgc cgccgaagcc
    16081 gcgccggccc ccttgaccac aggcgattct gccgccggcg cccccctcca acaattttcg
    16141 accctgcgcg agcagcgccg tcaaaaagcg ttggaagatg cccacgccgg catggcgcaa
    16201 atcgcctccg aacgcgacgc gtgggaacag gaatacgccg ccaataagga agccaaggcg
    16261 caggccgaag cacaggccct ccaagcgcag gccgagcgct tggcagccac tgatcaggaa
    16321 gcgctggaac agcgcaaaaa ctccttccgg tcccgcgctg tcaatgtgct ggcgggcgtc
    16381 gcctcggcca ccgtcagcgg cttcaccggc gccatcgccg gccgcgccgc cgacgaagcc
    16441 gtccacgcca tcttcgaccg caaataactc cgccccgcac cagttttcca cagtgtggaa
    16501 aaaatttatc ccgcgtgggc gggatttttc cacagcgtgg aacttttgcc gcggattttt
    16561 ccacagtgtg gaaaaaatga cccaaagttg ttccattgtt tggaaaaatc gctgaaacgt
    16621 tgccgctgcg ctcgctatgg ctcgctggct cgcccggcta cgccggtcgc tatagagctc
    16681 gcgttcgccc agcgcccttc gggctcgggc tcggcactcg cctattttcc attgtgtgga
    16741 aaaaaatcag ccctgcgccc catatttttc cacggcgtgg aaaactattt tccacaccgt
    16801 ggaaaaatgg ttggccgcgc cccgggcggc ttgaccacag aaaagtgatt ggggaatact
    16861 ggttgccaac tgcaagggcg gcagcccggg agaacgaata tgtggatctt gcgcggattg
    16921 ttgggaatcg cggtgctggt ggcgctgtgc tgggtggtga gcgaaaaccg ccggcgagtg
    16981 caatggcgcg tggtggcgtg gggactggcg ttgcagtttt tatttgcgct gttgctggtc
    17041 aaggtgccgg tatgccagca gattctcgcg caactgaatc gcggtgtgcg cgtcattgaa
    17101 cgcgccagcc atgagggggg ccaatttgtc tttggttatc tgacgggcgg ggccgcgccg
    17161 tttctcaccg cagatggctc cggggccgcg ccggcgctct ttttcttcgg gccgctgatg
    17221 atgctgattg tggtgggggc gctgagcgcg gtgctgttcc attttggcat tctgcaaaaa
    17281 gtggtggcgg cgtttgcgcg gctgctgcgc aaaaccatgg gcttgggcgg cgcggagggc
    17341 gtggtagtgg cggccaatgt gttcctcgga caaagcgatg cgccattgtt gatcaagccc
    17401 tatgtgggcc atctgggccg cgccgggttg atgtgcgtga tgaccgccgg catggccacc
    17461 atctccggca cgctgatggt ggtgtacgcc gcgattttgg agccggtgtt gccgggcatt
    17521 attctgggcc acctggtggt ggcatccatc atcagcgcgc cggcggcggt gatggtggcg
    17581 cgggtgatgg tgccggccaa tgacgccgac actgaccacg aaattcccga tttgaaggcg
    17641 gagacccacg gcgtggtgga tgccgtattg agtggcgcga acgatggcgc caaagtgatg
    17701 gtcaatgtgg ccatcatgct ggtggccatt attgcactgg tggcgctggt caacaagttg
    17761 ctggggctgg tgccgctggc cacgccgctt tcgctggagc gcatggcggg gtgggtgatg
    17821 gcgccggcca tgtggctggt gggggtgccg tgggcagagg cgcaagccgc cggcgagctc
    17881 atggcgctca aaacggtctt caacgaattc gttgcctatt tggcgctggc caagctgcca
    17941 ccggatacgc tcacggtgca cagccggttg atcacgattt attcgctctg cggttttgcc
    18001 aatttcagca gcatcgggat tctggccggg acgttgacgg cgctttgccc ggagcacaag
    18061 gccacggtgg ccagcctggg cctcaaggcc atggttgcgg gcaccattgc cacgctgctg
    18121 accggcgcgg tgatcggcat tttgatccca ccgtaaccgc gagcctccgt aaaaaaactc
    18181 gcgcggtcaa accggcgctg atggtggcgg tgtctttgcc ggcggttcat ctttcggctt
    18241 ggccccgccg cgatccgacg ggttgatcaa atccttttcc gcattgaaca cgccatccat
    18301 ctggagttcg atcggccgct ccttggcgtt tttgtaggtg gcggcggtgg cgccaaagcg
    18361 gagaatcccc aatttctgca atatcctcaa gatacccata acgcctcccg tgtcacccgt
    18421 ggttgttcat ttggcctcaa aggaaataat cggcagccaa agagggagcg tcaaggtcaa
    18481 ttgccatggc gaccgtacgt tgttccggag ggtggattca gtgttccgcc gctgtttctg
    18541 tcgcggcgag ccgttgagcc acgagttcct tggcctgctc gcggagttta tatttttgga
    18601 ttttgccgct ggccgtcagg gggtactccg tcacgaaaaa gacataacgc ggcaccttgt
    18661 aggcggcgag gtgctgacgc acgtaatccc gcacgtcttc ttctgtgagg ctctggccgc
    18721 ggttcagaat gacgaaggcg accggcagtt cgccatattt ggggtcgggt gcggcgacga
    18781 tttgggcgtc catcacttcc ggcatggcaa gcaacaaatc ttcaatttcc ttgggcgaga
    18841 tgttttcgcc gccgcgaatg atgatgtctt tgatgcgacc ggtgatccgg taatagccgt
    18901 cggcatcgca agtgccgaga tcgccggagt gcagccagcc ggcggagtca atgacggcgg
    18961 cggtttcctg gggcattttg tagtagccct tcatgacgtt gtagcctcgg cagcagattt
    19021 caccctcggc gccggggggg aggggggtgt tggtggcggg gtccagcacg gccacttcca
    19081 cgccggggaa tgctgtgccc acggtgctta cccgcagcgc caccgggtcg tgcccgtgcg
    19141 attgcgtgat gcccggcgat gcttcggtga gtccatagac cgaggtgatt tccgtgatgt
    19201 gcatgtcgct gatgacgcgt ttcatgactt cgatggggca gacggcgccg gccatgatgc
    19261 cggtgcgcag gctggtcatg tcgaacatct tgaacatggg gtgagtgtac atggcgatga
    19321 acatcgtggg cacgccatag agcaccgtgc atttttcctg atggatggcg gcgaggccca
    19381 agagggcgtc gaagacttcc accaccacga gcgtggcgcc gtgggaaagg atggccatga
    19441 ccccgagcgt gacgccgaaa cagtggaaaa gcggcacctg caagcaaagc ttgtcggcgg
    19501 ccgtgaaacg ctgccggtgg ccgatgctcc agccattgtt gaggatgttg tggtgcgaga
    19561 gcatcacccc tttggggaag cccgtcgtgc cggaggtgta ctgcatgttg acggtgtcat
    19621 ggcaggtgat ggctgcgcgc gcgtcggcaa gcaggcggtc gggctggtga tcgcccagca
    19681 gcatcagctc agcagtggtg tacattccgc ggtgcttttc ctggccgacg tagcaaacgt
    19741 ggcgcaggtg gggataggcc ttgctgtgga ggtggccgcg ggggtgctgc ttcagctccg
    19801 gcacaagctg gttgatgatt tgcaggtaat ccacgtcgcg aaaaccgtca atgagcccga
    19861 gcattttcat gtcggactgg cgcagaacgt aatccatttc gtcggctttg taggcggtgt
    19921 tgaccgtcac cagcaccgcg ccggttttgg cgcaggcaaa ttggaaggtc agccaatccg
    19981 gcacgttttt ggcccaaatg ccgacgtgat cgcctttgcc gatgccaatg gccagcagcc
    20041 ccttggccag gcggttgacg cggtggtcaa actcgcgata ggtccagcgc aggttgcggt
    20101 cgggatacac aatgaactcg tggtccggcg tggtacgcgc ccagaactcg agctgctcgc
    20161 caagggtcag cgtggtgaag ccatcggcat ccgcgccgat gggctggtgg gtggcgtcgg
    20221 gggggcggtg gggcatgatg ctgttcctaa acggttggcc gctcaagccg gcgcgtagac
    20281 caccgcgagg atttgtgccg cgccatccag cgcgtggaca tggtgcggga caaccgattc
    20341 gtagtaaatg ctgtcgccgg cgtcgagttg ataggactct ttgccataac ggatttcaat
    20401 gcggccggag aggacataaa tgaactcctc gccttcgtgc tcgttgagga cgatttcccc
    20461 ttcggcgggg cgcacatcaa tcagaaacgg ttccatgttg cgcgcggctt tgcgcggggc
    20521 gcgcggatga aatgccaacg tgctgcgctt gcgggaaggg tcgttgcccg aaaaatggac
    20581 ggccggattg gcgtcgtttt tacgcacgat ggccgggcca tccaaagcct ggtcatccag
    20641 cagcgtgccc aatcgtaccc ccatgccgcg cgccagccgc aagagcgggg tcagcgacgg
    20701 caccaactcg ccgttctcca gcgcctgaat catgtccacg ctgcagccgg tgcgttgagc
    20761 cagggtttcc accgataatt cgcgatgctc gcgcagcttg cggatgcggg ggccaatgag
    20821 gttgggttct tgggacatgg cgttctccgt gggggggcgt tgcggcgtaa aaaaagctcc
    20881 gccggggcgg cggagcttgg cggggcgagg gttacacttg ctcggcgagg tcttcggcaa
    20941 tttcccgggc ggcttccaac gccatttcgc ggcgctgctc ggagtcgaag tgacgcgagg
    21001 catcctggcc atcggcccac gcaatttgga tgtcgtccac gccgatgtag gagaaaatgg
    21061 cgcggatggc gggggtcagg ccgtcggtgg cgtcgtcctc cttgtagaca ctatcggaag
    21121 aaaccagcag caccacggtg cgcaggtggt ggatggggat tacaccatcg gggccaaacg
    21181 tgaacagctt gccaggcacc agcacctggt ccagccaagc tttcaggatc gccgggaaac
    21241 tgttgagcca aaccggcatg gtcaacacca gcacgtcggc atccaccagc gcttgcgcgt
    21301 tgttgttgga gaaattggcc gcggtttctt cggctttgga ggggatatag gccgggttga
    21361 ccaccgggcc ccagaaatag ttcaacgcct cggaactcac gtagggcggc ttgttctgat
    21421 aaaggtccac gttgttgacc accacgtcgg gatttttctc catcaacgcg gcaaagaatt
    21481 caaacgccag cttcttggac accgaggtct ccgccgggcg cgggttggca ataacgtgca
    21541 aaacattcat gagttactcc tgttgggtaa aaatggacaa taggttgccg ttgatgccaa
    21601 aaagagccgc ggaacacaat gctaaagttt ggcgtcacga aatgcggtgg acaaagcccg
    21661 cccgccatga ataatcagca cgatttggaa agcgaggaga ccgatggata tccagaacct
    21721 gtttgccgag cggatcggcg gcgcccagtt cgggcaaagc acggccatct ataaatttga
    21781 aaagatcaaa cgcgccaaac gcgccgccca agccgcccat cccggggtgg aaatgctcga
    21841 ttttggcgtg ggcgagcccg accaaatggc gcccaaggca atccgccaag ccctgaaaaa
    21901 agcagtggat gatcccgcca accgcggcta tgccgataac ggcattgccg aattcaaggt
    21961 ggccgccgcc aaatatatgg ccgatttttt cggtgtgacc gatttggatc ccgccaccga
    22021 aattaaccac accatcggct ccaagccagc gctggccatg ctgccgctgt gttttgtgaa
    22081 ccccggcgat gccgtgttgg ccaccatccc cggctatccg gtgctggcca cgcacaccaa
    22141 atatttgggc ggcaccgtca tccccgtgcc gctgctggcc aagaacaagt ttttccctga
    22201 tcttaaagcg ctggaacaaa ccgatctttc gcgggtgaag ttgttttacg tcaattatcc
    22261 caacaacccc actggcgcgg cggccaccgt cgagtttttt gatgagttga ttgcttttgc
    22321 tcaaaagcac aacatcctga tcgtgcagga tgccgcctat gccacgctca tttacgggcg
    22381 cgagcggctt tccatcctgt cgcgaccggg cggtaaagag gcggccattg aattgcactc
    22441 catgagcaag agctacaata tgaccggttg gcgtctcggg tttgtcgccg gtgccgcccg
    22501 gaccgtggcc gcggtggccg aggtcaagga caacattgat agcggtcaat tcaaggcgat
    22561 ccaggtggcg gcctgcgcgg gaatcgcgga ccaaaagctg tcggaaaaaa tccgcgccca
    22621 ttatcagcgg cggttgcgcg gccttgtcaa agcgctgaaa ggcgccgggt tccatgcccg
    22681 gatgccaggc gggacatttt atctgtatgt gcccgcgccc aagggcaccg cggatggccg
    22741 tacctttgcc aatgccgagg aggcctcaca gtttttgatc acggagcatt gcatctccac
    22801 cgtgccttgg gatgatgccg gcgcatacct gcgtttcggc gccacgttcg agtcggccgg
    22861 acgggccgat gatgcccgtg tgctggcgga gttggcgcgt cgcctgcaag ccgcccagtt
    22921 ggtgttttga aggagcgcac catgagccaa gccaagatca tctttgccaa aggaacccaa
    22981 ctgacggtct acattgacaa ccgcaagggg acactggcgg cgctgaccac atttctggcc
    23041 aagcacgggg tcaatattta tgggctgaca ctcgccgata ccgaaggtca cggctacgcg
    23101 cggttgattg tggacgatac cgagaaggcc cgccaattgg ttgaggattc tggcgaactg
    23161 gtcgcggccc gcgaggtgct attgattcgg gtggccaacg agccgggcga attggcccgg
    23221 atgctcacga ctttggccgc ccacaatttg aacattgaat acggctactc tgccggcggc
    23281 cccggcgacg aaaaaggcct ggtacttgtg ccctccgatg tggatgccgc cttggcggtg
    23341 ttgaaggaac taaacgccgg tcattgacat gccccctgcg cccaaagtta gcatcatcac
    23401 cagcacgtat aaccgctccg cggtgctgcg gcgggcgatt gaatccgtgc gtgcccaacg
    23461 tgactttgac gactgggaga tgtgcgtggt aggcgattgc acccccgatg acagcgcagt
    23521 ggttgtcgct tcgtttggtg accctcgcct tcggttttat aatttgccgg aaaaatctcc
    23581 gccgcgcgcc catggcgcca tcgccaaaaa ttatggggtg ttccaaatgg cccgcgcacc
    23641 ctacatcgcc tatctcgacg acgacgacga ataccacccc gactttctgc gcgtgatgat
    23701 gggtgtagtt gcccgcaccc ccgaacagcc ggtattttat tgtcggtgcc aatatcgtga
    23761 caagaaaact ggccgccgaa tttggggtaa tcccttccag ccttggttgc atcgctggag
    23821 ccgcaaaaag ttgctgcggt ataacttcat caacaccgcc aatgtggtcc acaaaaagtc
    23881 gctgattgat gaagtcggcg gctgggaccc caacgactat tttgacgact acgacttgtg
    23941 gaaacgcatg agcgcccgct gtgattttca ctatgtgaat cgcgctctgg tcatcagcca
    24001 cgtcggcgat tgcccgccgt tctttgtgcg cttattcacc aaaggatgga aaatcctccg
    24061 ttacggccgt cggctgcaag accaaggttg ataactagca ggtcatccct tgctgaatta
    24121 ggcgtatgag cgctttcgcc agcgggtgtg ggggcgtaat caattgcact tggcgttcca
    24181 gtatcacaat aaagccgccg gcttgtagtg tttggagatg aagataaaac ggccggatgg
    24241 gaatgcggct gttcattagc agttcgccaa gaggcaatgg ttgctgcaga agttggcgta
    24301 tgaggcgtat tcgccgctca tgtgccaaag cattgccaat gttcaccatt tcaatatcgc
    24361 gttcaggcgg cagggtcgcc agtgactctc tgatggcttt ga
//
LOCUS       DAOPNP010000025        24096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2629_length_24096_cov_6.654923, whole genome shotgun sequence.
ACCESSION   DAOPNP010000025 DAOPNP010000000
VERSION     DAOPNP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24096
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_24096_cov_6.654923"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1827
                     /gene="ilvD"
                     /locus_tag="PKI64_03690"
     CDS             154..1827
                     /gene="ilvD"
                     /locus_tag="PKI64_03690"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE00146.1"
                     /translation="MATKSRISDQVLKGPERAPHRSLLYALGLSREEMNRPLIGVVSA
                     FNEIIPGHIHLDKIADAVKAGVRMAGGTPILVPAIGVCDGIAMAHPGMYYSLPSRELI
                     ADSVETMALAHCFDALVLIPNCDKIIPGMLMAAARINIPAIVISGGPMMAGDLDGRHL
                     SLSNMFEAVGACKAGKISQNELTDYEETACPGCGSCSGMFTANSMNCLSEAIGMALPG
                     NGTIPAVAAARYRLAKQAGMQIMQVLAKNLRPRDIMTSDAFTNALRVDMALGCSTNSV
                     LHLCAIAHEAGVPMSLDRINAISATTPNLCRLSPAGPDHIQDLHLAGGVPAVMKELAA
                     AKLLNTKLMTVTGQTVGRNIAGAANRNPSVLRPVKNPFSPTGGIAVLKGNIAPRGSVV
                     KRSAVAPEMLVKTCKARVFDSEEAAIASIYAGKIKKGDVVVIRYEGPKGGPGMREMLS
                     PTSALAGMGLDKDVALITDGRFSGATRGSAIGHVAPEAASGGPIALVKEGDKISIDIP
                     AGKLNLLVSAAELKKRAAKLKPFKPRITTGWLARYQRLVGPADIGAVLQ"
     gene            1993..4542
                     /locus_tag="PKI64_03695"
     CDS             1993..4542
                     /locus_tag="PKI64_03695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE00147.1"
                     /translation="MAQNPVDSLQAAQTGIRRVVLDNGLVVLLKQDRSAPLVAIQYWV
                     NAGAIHEGAQLGGGLSHYLEHMVFKGTAKRGPGQVSKEIADIGGEINAYTSNDRTVFH
                     VVLPSAHWLVGLDVLTDAVFHPSFPAEEWEREREVILREVDMGEDDPMRVFSRLVNET
                     AFRVHPYRVPVIGWRDILITMNRDHLVAYHQRHYSPDNMILAIVGDVALDDMEAAVRE
                     QMREIPRTPREPVLIPAEPPQLAEQYGRKTGPYNVTRVGWVFHGTDLANADAPALDVL
                     ASVIGSGRASILVKKFREEEQLVLDIEAWNYTPKEPGLFGITFECEPAQEAAALAALR
                     AEVASWHSTLMDPARIEQARREVLAGAIQTLTTMEGQAQSFASGEFYTGDPRFNETYL
                     NLVQQVTPADLQRVVQKYLRPENGSLAILAPASEEPTTQAPATSKDAAIQLHTLANGM
                     RLLVREDPRLPMAYVSVVIGGGVLGEQAGQAGISQLTAELLLRGTSQLSAAELASKLE
                     SRAVSLSAYSGRNTYGLNAAGLSEDLPLMLNTVAECLIDSQFPADEIEKQRAFQLADI
                     QRSLEKPMTHAQQMVRDAFFPGHPYQYSSLGNTESVSALTRDDIVAHHRRLLVGSNVV
                     VAVFGNINAAEVVAQVETGFAPLPPGVAPTWPKLPDTVTEPAQLEKGLPFKQTVIVRA
                     WRGIAAGDPREDALSVLADTLSGLSSDLFIEVRDKRGLAYYTGASQLTGPVGGLFMIY
                     SGTTTEGRTEVCRQIQVQTDRLSQSGPRPEEFARAVEQMVAGQARSLQNNSGLAQQCA
                     IDELLGLGWQHSFQMIERLRQLQPEDVRAIAQTIFGNAGDVTVVVLPADIP"
     gene            4539..5276
                     /locus_tag="PKI64_03700"
     CDS             4539..5276
                     /locus_tag="PKI64_03700"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE00148.1"
                     /translation="MTPGSQRKAAIAILALLGGGLLIWFWPDVRALLARVRWADIEQT
                     VREAGPWAPVLCIVLDAVFTVFSLPTTLVGVLIALLYPIVWGLLLCLIGLGLGMSATF
                     LLARYLLRDWLARRIGHTRLFDYLEANMRREGWKVVLFTRLLPINPFSFLNYAYGLTS
                     IPFHHYLLASVIGVIPNVLALLWTVRAAGQLARGQADWRVLAILFAGAGLFAIMAWLP
                     KLLRKNPSLAQLPLTHNQDLLADDPPE"
     regulatory      5346..5461
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5513..6730
                     /locus_tag="PKI64_03705"
     CDS             5513..6730
                     /locus_tag="PKI64_03705"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOE00149.1"
                     /translation="MVDLKQDPVFCTVEEALAIIRDGKMLLVVDDESRENEGDLVVAG
                     EKVTPEAINFMATYGRGLICVPMEEQRLCKLGLCRMVPLEQQDQFRTAFTISVDAREG
                     ITTGISAFDRAHTVKKLADEKSSACDFIRPGHIFPLQAVPGGVLRRTGHTEASVDLAR
                     LAGLKPIAVICEVMHDNGEMARLPDLVPFAQKHGLRILTVADLVAWRRARERLIRRDT
                     VVKLPTDFGEFMLYLYVDETSNEHHVALVYGEPAKQDAPLVRVHSECLTGDVFGSHRC
                     DCGDQLHAAMRQVVAAGHGAILYMRQEGRGIGLVAKMQAYALQEQGYDTIEANLKLGF
                     PADLRDYGVGAQMLLDLGITRLRLLTNNPRKIVGIQGYGIEIVERVPIAFPPNEDNAK
                     YLKTKKDKMGHMI"
     gene            6746..7216
                     /gene="ribH"
                     /locus_tag="PKI64_03710"
     CDS             6746..7216
                     /gene="ribH"
                     /locus_tag="PKI64_03710"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOE00150.1"
                     /translation="MNYKEYSGQLNAKGLKIGIVISRFNDLFTGQLLKGALDCLARHG
                     ADEKNVEVVWVPGAFEQPLAVKKLAQTKKFNAVLALGAIIQGATPHAGLIGTQVARAL
                     TQISLESGVPVVDGIVTAENLDQAIERCGSKSGNRGWLAAQTAIEMASLFQQLP"
     gene            7274..7774
                     /gene="nusB"
                     /locus_tag="PKI64_03715"
     CDS             7274..7774
                     /gene="nusB"
                     /locus_tag="PKI64_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE00151.1"
                     /translation="MGSRHEAREWALQFLFQSEFNREESLAEGFKLFWEHLNEPPAGT
                     GGQPAAKVDKQRTKAREFAEELARGVIAHHQEIDPLIAKYAENWEISRMGTVDRNAMR
                     IAVYEMLYRHDIPPVVSINEAVELAKAYSSVESGKFVNGILDRIRKGLDRPARDANRG
                     STPPQG"
     gene            7780..8679
                     /gene="ftsY"
                     /locus_tag="PKI64_03720"
     CDS             7780..8679
                     /gene="ftsY"
                     /locus_tag="PKI64_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOE00152.1"
                     /translation="MGSWFDSLRKTRARFADALAGIFSSTRADPASIEELTDLLITAD
                     VPMRLVTELTHELERTSSRKEPLLATFERVLVTALGENPQWSWSTQPKPVVILLVGIN
                     GSGKTTTAAKLARQAQQAHRNPLLGAADTFRAAGSHQLKLWADRVGCESVIGATGADA
                     AAVAYDAIDAAVARHCDTIIVDTAGRMHTKEPLMRELQKVRAAMAKRLPEAPHHTWVV
                     LDAMLGQNAVVQARQFHEITPLSGVIVTKLDGSSKAGFLFAVRQELNVPILFAGLGEG
                     MDDLASFDATQFVRALLQTEGAT"
     gene            8676..9767
                     /gene="ribD"
                     /locus_tag="PKI64_03725"
     CDS             8676..9767
                     /gene="ribD"
                     /locus_tag="PKI64_03725"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOE00153.1"
                     /translation="MNLASDQVWMQQALAMARRGLGLTRPNPPVGAVIVKNGRKIAQG
                     YHRRAGGPHAEIVALRQAGSAARGATLYVTLEPCSTHGRTPPCTEAILRAGIARVVAA
                     TTDPNPKHAGRGLRLLRKAGLEVRLGVCRVEANTLIAPFACHMLHHRPFFTLKLGLSV
                     DGRIADHTHSSRWITGPAARKEVQALRRAADAIMVGAGTVRHDNPSLWPRPDGQRNPW
                     RIIIAGKGPLPLDAQVFNDEHAKRTIVAAPRGWQPSCAQIIRGKGAAVLELPAKSLLA
                     SLARELGNLGIMHVLCEGGGILAGDLLQAGLVDELCIFFSPVLIGGPVGAMGLAQWKL
                     NVAPRFSLRESRRVGHDLFIRMTPATAER"
     gene            9768..10412
                     /locus_tag="PKI64_03730"
     CDS             9768..10412
                     /locus_tag="PKI64_03730"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOE00154.1"
                     /translation="MFTGLIQQIGRLTEITFQGDAGRLSLMAKFTAPLQIGESIAING
                     ACLTLVAQQEDLLTFDVLRETFEKTGLRGKPKGAPLNLERALRMGDPLGGHLVSGHVD
                     GIGLLQRITAAGRDKVLQVHAPALINEMVPKGSIAIDGVSLTLVEVRSADGVFTVHVI
                     PHTWDNTAFNSLRPGDQVNLETDMLGKYVRQALFNAAQNSAVSWDKLRSAGFLP"
     gene            complement(10422..11138)
                     /locus_tag="PKI64_03735"
     CDS             complement(10422..11138)
                     /locus_tag="PKI64_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00155.1"
                     /translation="MKLWFGLAILAGLIEMWQLKIGRSAEWSDWFYGLLGATGAIGTA
                     KLHSKLRVAVLLGLALIPPLWIGTLLAQEARAFPVLLDPQSIWARQGWTQQGGQVQPL
                     DFSKFCFRANSSGEEVEYPGLFRPVFHCDWRGITALRGSIYWPNAMPTICAIRVDDQR
                     GNPPYADRFQREMAITQGWNQIVIPSAELRMTPSGRELQMEQIQQWGIFLIAPPQFEN
                     FVLGCFALELGGGLNEEISL"
     gene            complement(11333..24052)
                     /locus_tag="PKI64_03740"
     CDS             complement(11333..24052)
                     /locus_tag="PKI64_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE00156.1"
                     /translation="MGGTGLPWATTNYLSTAQGVWRVCTTNSSVCGQTETLFAIKNDP
                     VAYYVNDNSTVGDVYCTGPGRATNSGLSAAAPLNSLATLLSRYKVEHGDTVYVDTGVY
                     PRTAPLTIAISAIAPTNRLIIQGSTNEAAGGTVFTNASGTILEILNSSNIELRDIRLH
                     GGSQGLVLNRSSSNRFVGVRSMNAAVNAFVISVQSGENEFIRCAALNFSQTGFYMAPI
                     MSQQIAPVTNFWSQGVLSPLPASNGIALTTGTLIGVESGCLYVSNSALIANSPAHEIF
                     RLGAGTVAGDYNLFHQPHANTIFAKTPRGGSVFGVQTIQFRDLTEWQEWTQGQSNSFM
                     ADPLFANLVTGNLYPRSQAGRYDPTTQDYVLDAETSPLIDSADPALPWAAEGEPNGSR
                     ANIGIYGGTPAASRSPTNGSFVLLTLNQGGTASGTQRLRWIARGAVTNPSHRVNVQLS
                     TNSGASWQNIGVSTGLVGFHEWDTTSLPPLTACRWRVQSQSQSTWVTASERDFMVRNA
                     PWTFYVNDAATNGDIYTTAIGAITNSGLHPSSPLPSLAAVLTTYDVRPGDLILIDTGI
                     YTNSTPVIIGYMDRGEIAEPVVIRGSTNAAGSVFTQNGLNLVNTRGLKLEHLRFENMM
                     AGAAMVSASEDISLQNVDILGNVGSGITISQSSNILVRNFAVTGAQTNGVVCEASYGT
                     TLEFGVVWSNVSAQVVVNNQLQQGGANSAYNDAYTTISNCIIGSFGIRVPAYLLNGTL
                     VSDYNNIYLGNGGLAALSYTGGFAREYDSVGSWVGTNGFYDSHSLSHSPLFVDAAAGD
                     FHLMSSAGRWVPAANQWTNDAVTSPLIDAGTPSLACTEPEPNGARVNIGRYGNTAQAS
                     KTPTNSALTLITFNDGGRASGNNVPIQWNARGQITNGTISIYYSADGGGTWSLLTNGL
                     AANSGVWNWDSTASEQSVQAKLKIEGSDGSSTQSDKVFSVRNAPFYFYVNDGSTIGDV
                     YCTAIGQGANSGLSSNAPMADLNALLAKYDLESGDVVYIDTGVYATGVDPWRITQADS
                     AGALNVPPVVFQGSTSSLMGGTVLQRNYNSVGLQADYVIGVVLRNIVVSNTTRTAFAL
                     NQARDVALEWCAVGGGDVGVRLNSGNNLRVEHCVVVDANTGVVVDNWDRSTNTVFPVI
                     NNNVLWNLGQSAIKISELHRATVMNNIMNITGENYIYDLADTANLVADYNALWLGTHG
                     RVARRMQGGDRSRYPLVYETVGAWATSSSNDLHSYDGDPLLVNPSNRIFHLKSRAGRW
                     NPIAGSWTNDAQTSPLIDMGEPSAKTWTNEPAPNGQRLNVGLYGGTSLASKSETNSAL
                     CLLTLNHGGVVTGPVALNWLATGMATGHTVRVEVSNDDGETWHLVVPSVSATLGGVKW
                     NSSSLASSPLGRWKVTDLDEPMVTAISARPFVIHHSAITYYVNDDHADGDVYCTELGN
                     STNTGISPDSPKRWVAEIIDSYNLEPGDTIYVDTGTYSMSQPTVFGDLDAGDFIQAAD
                     RQVNVIGSTNALAGGSLFVMTDPTVNAFELKNTYGIRLAHLGILNASNGIYAQDSRMI
                     AVEWLEMRNCANGISSVDSSSMQVRHSTFVGNQNAGVYVAAVEMGTVDIQSSVFWGNR
                     AGCYLAQGYAHVSNSIVGITRPNSYGIYWHADKPLTGYSGDYNNLFVGHPNAAIGALQ
                     TGASSSARTNVYAFLSKWAADLGADTHSLAQDPLLAEPGYDFHLKSAGGRYQPGTGWV
                     TDPVSSPMIDAGDPRSLGWTVEPLPNGTRVNIGLYGGTTEASKTPSNGILTCVFPTEG
                     GQVEKDIVLQWSAIGAATNHSVLLEYSSDAGVTWSNIVSGWPAESGSYKWNSVPYGRS
                     ARAWWRITSLDDYSIRDQAGPFMLKNGGMIPFYVNDTNMTGDVYCTAIGDDINDGLTP
                     ETPKASLQAIFDSYDLAASDVIYVDAGTYLAGSPPIKINEKDSGHSANGTNYFITVQG
                     STNPVAPTIFIAPSFSAPQVFALDYAVNIRLKDLTIRNAQTGISAYKTIGLELDGVRV
                     ENNRITGLTIDDCEETRMIRSVLWKNASPTGGIAIALNKGDLCVENSVLWGSYSGITV
                     GSGSLQITNSVLYASGPNGRIYNYGISSSIGGGGFRGDYNSYARWNGALIAEQALQVG
                     GSDYFNDLPTWSKRVGSDRHSMTATYSLEPAFADEITGDFHPKSTKGRFIPASQTWTN
                     DAVRSPLIDAGAPDYAADREPEPNGGMINIGAYGNTAQASMSQTNPPWVQAISYNAEG
                     ILSGEALLYWTFGGLPDGALVRLDYSPDYEVTWKPIASNVPAATREFLWDLSGLPLSL
                     GMQWRVMYQGNTNIWDVTDVPIPHNPPGTKTFYINDNSTNGDVWCSGPGLPFSAGTPY
                     GTNAAQPLNSLADLLANYPVGPGDIIYVDTGTYPLSKPILINERHQGTTSMPVRIYGS
                     TNISAGGAVFQGVENKLFAIQNTRNIRLQDIRVTQAQHGISINNADNVVLIGMELWDN
                     QTNGLYVSGSANITIQNARIWRNGGSAYASAGSKGGETIINSTIYGNRGGGISTDKGL
                     TLVNSIVVTTNASAIFVESAQSASIGGDFNLYFFPPNGVLCSNLSEKIDYKNLSEWQT
                     KNKDTHSVVIDPLFVNPAAGDFHLQSTGGYYSNGTWAISTNTSWAIDAGNPTSSFTNE
                     PAPNGGRINLGAYGGTAQASRSDTNHPALLPITLKDGGVAPYGQPLYWLSRGISPTNL
                     VQIEYSPDGGATWLFVANGIPVGSQPYMWYSADPPSPLALWRIFLMTDTNIIGTTTVP
                     FTFRPTPLIYYVNDTDTTGDIYTTAPGAKTNLGYRADSPRDTIQSILEQYRLVGGDSI
                     RVDTGSYTLTNQVFISPKASGSGTNQVLILGSTNLAAGGTLVQAGINMRAPAFEFYGA
                     HDVRLANIRIKGFTNGVSVAEQSSRCVLESLDIQESFGSGVVLNKSQDITLRRVSIRH
                     GTASAISLAQGKAMIESCVLWSNAASAVSLGSGADLGITNSVVSAFGLASYCYESPTN
                     ITIQANYNAYFLTNGAQMASIDGVQYESLPQWVRAKAPIDRYSLHTDPLFADAATGDF
                     HLKSSAGRYQLGVGWVQDPPVNGQPVFSPLIDMGAPTTGWTSEPAPNGTRRNIGLYGN
                     TAEASKSNTNRWLQAITAMSGGILFGDTVLTWGYSSLIPTNTKVQLEYSYDGNWLKIG
                     EATVSDGAFYWQSDLRNVDGTERWTSSPAEYWRIFLLNDTNVWDATDRPFGLRNSPFK
                     YYVNDLSTVNDVYTTAIGSDTNPGYYARAPKLTVNALLEDIDIEPGDMVLIDTGIHYM
                     TDTNRPIIWQVSDGGTTNTPVIVRGSTNPAGSWIVATNRFTAGAFMFMEANHIDIRDL
                     GFVGESVRFLGDSITASNLWVTNGTVTINGENAVFKKLQLDRGNLSFSGSNNRIERME
                     QRWGQASLVGTNITLLNSVVFTTNNLTTALVVNALGATVSNSTVVATRGTAVGKLGAG
                     TLRLGHNILVAGGSDSNSVLAWHDGGLISDWNNFVARDSAWVGIKEGRWETLGYWQAA
                     SGRDANSLAIEPLFQNEAAGDFHLNSKMGRWSPIFKDWDIDPQHSPAIDAGNPQIGTG
                     DEIGPHGYRPNLGAYGRTLEASKSITNFWLTAVTQNDGGVLKGSNVTLRWLAGNASGK
                     TVSLEYYDGSAWHTIATGVNATDESYPWDTTGFPDSFAAYWRVVAEDASGTSDQTDHP
                     FQLRNHTHKFFVNDSSLLGDLYCTAVGSDSNSGLSNSAPKATLQAILDAYDLEGGDTV
                     YVDTGTYASTSDVKVIWSRSGNQTADVVIRGNTNDPYATQLVRLGQTNYPAMGLDVKA
                     SHIRLTDFSISGVNRAIGLESNVGVRASGLVLHDVGIGIQALGTRNTDIRNSGFWNAE
                     MGISLSNTRTSILENLTFVNTSLAGIQLGNTEVVTLRNNIFIPAPGSYAYAIGSATSL
                     LAQAILDYNLYDFGDEESGSGFFAGATNDLRRWQLEMNRDFRSAITNADLANLDLGDF
                     HPLSEYGRWTDSGWTTDGITSWAVDHGNPAADYSQEPEDNGQRINIGMYGNTVQASKG
                     SIDIELYARTMNEQPLMVTQEDQVWPWVWSAHLLDTNEMVSVQWSGDCGETWTTLTNV
                     NAYQEYYIWRAGIEYQTAGGRWRVISQSNTNIVDMSDTCFLVRWLPPAYLKVYPSSGR
                     IRFDWQGGVQGRRYVVEYSDDFGQTWVTWDEKYNGPAQINRSNFVIPSGGSQLKYTFE
                     DRTSYLRRTRWYRIMVYEE"
BASE COUNT         5429 a         6830 c         6380 g         5457 t
ORIGIN      
        1 gcccaaccac cagaaaaaga aaaagtaagg cgcttgcccg gatgggcgtg gcggcatgga
       61 tttttcgcaa gcgccccaca aaacgcttga aaaatatggt cggctggcgg acagtcgcca
      121 cccggaactg catacaaatt aaggatatag attatggcaa cgaaatcccg catcagcgat
      181 caagttctca aggggcccga acgcgcaccg caccgctcgc ttctgtacgc gctgggtctt
      241 tcgcgcgaag aaatgaaccg cccgctcatc ggcgtggtgt ctgccttcaa cgagattatt
      301 cccggccaca tccatctgga taaaattgcc gatgccgtca aggcgggtgt ccgcatggcg
      361 gggggtacgc ccattctggt gccggcgatt ggcgtctgcg acggcatcgc catggcccac
      421 cccgggatgt attactccct cccctcccgc gaactgattg ccgattcagt cgagaccatg
      481 gctttggctc actgttttga cgccttggtg ctcatcccca attgtgacaa aatcatcccc
      541 ggaatgctca tggccgcagc gcgcatcaac atcccggcca ttgtcatcag tggcggcccc
      601 atgatggccg gcgatctcga tggtcgccat ctcagcttgt ccaacatgtt tgaggcggtt
      661 ggcgcatgca aggcgggcaa aatcagccaa aacgaactga ccgactacga agaaaccgct
      721 tgtcccggct gcgggtcctg ctccggcatg tttacggcca attccatgaa ttgcctgagc
      781 gaagccatcg gaatggccct gcccggcaat gggaccattc cggcggtggc cgccgcccgc
      841 taccgcctgg ccaagcaggc cggcatgcaa atcatgcagg tgctcgccaa gaatttgcgc
      901 ccgcgcgaca tcatgaccag tgacgctttt acgaacgcat tgcgtgtgga catggctcta
      961 ggctgctcaa caaatagcgt gctgcacttg tgcgccatcg ctcatgaagc gggcgtgccc
     1021 atgtcgctgg atcgcatcaa tgccatcagt gccaccaccc ccaacctctg ccgtttgagc
     1081 cccgctggcc ccgaccacat ccaagacctg catttggcag gcggcgtgcc ggcggtgatg
     1141 aaagaattgg ccgcagccaa attgctgaat accaagctga tgacggtgac cggccaaacg
     1201 gtcggccgga acatcgcggg tgcggccaac cgcaatccaa gtgtgctgcg cccagtgaaa
     1261 aatccgttca gccccaccgg cggtattgcc gtcttgaagg gcaacatcgc cccgcgcggc
     1321 agcgtggtga agcgctcggc ggtcgcaccg gaaatgctgg ttaaaacctg caaggcgcgt
     1381 gtcttcgaca gcgaggaagc cgccattgcc agcatctacg ccggcaaaat caaaaagggc
     1441 gatgtggtgg tcattcgcta cgaaggcccc aagggtggcc ccggaatgcg tgaaatgcta
     1501 tcgcccacct ccgcgctcgc cggtatgggg ctcgacaagg atgtcgcgtt gattactgac
     1561 ggccgtttta gcggcgccac gcgcggttcg gccatcggcc acgtggctcc ggaagccgcc
     1621 agcggcgggc ccatcgccct cgtgaaagaa ggggacaaaa tctctattga tattcccgcg
     1681 ggcaaattga acctcctggt ttccgccgcc gaactcaaaa aacgcgcggc caaactcaag
     1741 cccttcaagc cgcgtatcac caccggctgg ctcgcgcgct atcagcgctt ggtcggccca
     1801 gcggacattg gcgcggtttt gcagtaacgc agcccccccc agtcgccatc gcgtcttgcg
     1861 cgatggcggc tttgccttca cgccggcgga tgctttgctt tcgcccgtgc gcatggtatc
     1921 ttccggccca tgaatcgttg gcggcaaatc cttggccttt cgatggcctt gtttttcgtt
     1981 caggagtttt ccatggcaca gaatcccgtt gattccctgc aagcggcgca aaccggcatc
     2041 cgacgtgtgg tgttggacaa tggtctggtt gtgctcctga aacaggaccg cagcgcgcca
     2101 ctggtggcca ttcaatactg ggtcaatgcg ggtgcaatcc atgaaggtgc gcaactgggc
     2161 ggaggcctct cacattatct ggaacacatg gtgttcaagg gcacggccaa gcgcggcccc
     2221 ggccaggtgt ccaaggagat tgcggatatc ggcggcgaga tcaacgccta cacgtccaac
     2281 gaccgcacgg tatttcatgt ggtgctgccc tcggcgcact ggctggtggg cttggacgtt
     2341 ttgacggatg ccgtattcca tccatctttc cccgccgaag aatgggagcg cgagcgcgaa
     2401 gtcatcctgc gcgaagtgga catgggcgaa gacgacccca tgcgcgtgtt cagccggcta
     2461 gtcaatgaaa ccgcctttcg ggtgcatccc taccgcgtgc cggtgattgg ctggcgagat
     2521 atcctcatca ccatgaaccg cgatcacttg gtggcctacc atcaacgcca ctattccccg
     2581 gacaacatga tcttggccat cgtgggcgat gtggcgctgg atgatatgga agctgccgtc
     2641 cgcgaacaaa tgcgcgagat tccgcgcaca ccacgcgagc cggtattgat ccctgccgaa
     2701 ccgccgcaat tggccgagca atatggccgc aaaaccggcc cctataacgt cacccgcgtg
     2761 ggctgggttt tccacggcac cgatttggcc aatgccgacg ccccggccct cgatgtgttg
     2821 gcttcggtga tcggctccgg acgcgcctcc atcttggtca aaaagtttcg cgaagaggaa
     2881 cagttggtgc tggatatcga ggcatggaac tacacgccga aagaacccgg cctgtttggc
     2941 attaccttcg aatgtgagcc tgcacaggaa gcggcagcgc tggcggcctt gcgcgcggaa
     3001 gttgcttctt ggcacagcac gttgatggac cccgctcgta ttgaacaggc gcgccgggaa
     3061 gtcctcgccg gggccatcca aaccctgacc accatggagg gccaagccca gtcctttgct
     3121 tccggcgagt tttataccgg cgacccgcgc ttcaatgaaa cctacttgaa tctcgtccag
     3181 caggtcaccc cagccgattt gcagcgggtg gtccaaaaat atctgcgccc ggagaacgga
     3241 tcgctggcaa ttctcgcgcc tgcgtccgaa gaacccacca cccaagcccc ggcaacttcc
     3301 aaggatgccg ccatccagtt gcacaccctc gccaacggaa tgcgcttgct ggttcgcgaa
     3361 gacccccgct tgccgatggc gtatgtctcc gtggtcatcg gtggcggcgt tttgggcgaa
     3421 caggccggtc aagcaggtat ttctcaactg acggccgaat tgctgctccg cggcacatcg
     3481 caactttctg cggccgagtt ggccagcaag cttgaatcgc gcgcggtttc gctcagcgct
     3541 tattccggcc gcaacaccta cggcctcaat gctgcgggac tttccgaaga tttgccgctc
     3601 atgttgaaca ccgtcgcgga atgcctgata gactcgcagt tccccgccga tgaaatagaa
     3661 aagcaacgcg ccttccagtt ggctgatatt cagcgctcgc tggaaaaacc catgacccac
     3721 gcccaacaaa tggtgcgcga cgcctttttt cccggccacc cctaccagta ttccagcttg
     3781 ggcaacactg aaagtgtcag cgcattgacc cgtgatgaca ttgtcgcgca ccatcgccgc
     3841 ttgttggtcg gttccaatgt ggtggtggct gtttttggca atatcaacgc cgcggaagtg
     3901 gtggcacagg ttgaaacggg ttttgcgcca ctgccccccg gcgtcgcccc cacttggccc
     3961 aagttgccag atactgtgac cgaacctgcg caattggaaa aagggctgcc cttcaaacaa
     4021 accgtcattg tgcgggcatg gcgcggcatt gcggcgggcg atccccgcga agacgcactg
     4081 tcggttctgg ccgacaccct gagcggcctg tcctcggatt tgttcatcga agtgcgtgat
     4141 aaacgcggtc tggcctacta caccggggcg tcgcaattga ccggcccggt gggtggtctc
     4201 ttcatgattt attccggcac cacgacggaa ggccgcacgg aagtgtgccg gcaaatccag
     4261 gtacagacag accgattgag ccagtcgggt ccgcggccag aagagttcgc tcgtgcggtt
     4321 gaacaaatgg tcgccggcca agcgcgcagc ctgcagaata actccggctt ggcgcaacaa
     4381 tgcgccattg atgaactgct tggcttgggc tggcagcatt cgttccagat gattgaacgc
     4441 ctacgccaat tgcagccgga ggatgtgcgc gcgatcgcac aaaccatctt cggcaatgca
     4501 ggcgatgtta ctgttgtggt gttgccggca gacattccgt gacccccggt agccagcgca
     4561 aggcggcaat cgccattctt gccctgctgg gcggcggatt gcttatttgg ttttggccgg
     4621 acgtgcgcgc gctgctggca cgcgtgcggt gggccgatat tgagcagacc gtgcgcgagg
     4681 caggcccgtg ggcacctgtt ttgtgtattg tgctggacgc tgtattcacc gtgttcagtt
     4741 tgcccacaac actggtgggt gtcttgattg cgctgcttta tcctatcgtc tgggggctcc
     4801 tgctgtgcct catcgggctt gggctcggca tgagcgccac ctttctcctc gcccggtatt
     4861 tgctgcgcga ctggttggca cgccggattg gccatacccg cttgtttgat tacttggaag
     4921 ccaatatgcg gcgggaaggt tggaaggtcg tgctctttac acgcctgctc ccgatcaacc
     4981 ccttcagttt tctgaattat gcctacggcc tcaccagcat ccctttccac cattatttac
     5041 tggcttcggt aatcggtgtg atccccaacg tgctggcgtt attgtggacc gtgcgcgcgg
     5101 cggggcaact ggcccgcgga caagctgatt ggcgcgtcct ggccatcctg tttgctggcg
     5161 ccgggttgtt cgccattatg gcgtggttgc caaagctttt gcgaaaaaat ccctcgttgg
     5221 cacagcttcc gctcacccac aatcaggact tgctggcgga tgaccctccc gaatgagtcc
     5281 acttccgtac agggattttt tctgtttcca aggttgcaac aatccgggcg gcgtgatagg
     5341 ttgcggcttg tcttcagggc gtggtgaaat tcccgaccgg cggtacagcc cgcgagccaa
     5401 taggcagaca aggtgcgatt ccttggccga cagttgaaag tctggatgga agaagacgtt
     5461 gcgcgattgg ttcgcgtttg cgcccggaga cagaaaggtc ttcggttttt ttatggttga
     5521 tctcaagcaa gatccggtct tctgcacggt cgaagaagcg ttggcgatca tccgcgacgg
     5581 caaaatgctg ctggtggtgg atgacgaaag ccgcgaaaat gaaggcgatt tggtcgtcgc
     5641 cggcgaaaaa gttaccccgg aagccatcaa tttcatggcc acctatggtc gcggcttgat
     5701 ctgtgtgccc atggaggaac agcggctctg caaacttggc ctttgccgaa tggtgccatt
     5761 ggaacaacag gaccagtttc ggacggcatt caccatctcc gtggacgccc gcgaaggcat
     5821 taccaccgga attagcgcct tcgaccgtgc ccacaccgtg aaaaaactgg ccgatgaaaa
     5881 atcttcagct tgcgatttta tccgcccggg ccatattttc cccctgcaag cggtgcccgg
     5941 aggcgtcctg cggcgcaccg gccataccga ggcatcggtg gatttggcgc gtttggccgg
     6001 cctcaagccc atcgctgtca tttgcgaagt gatgcacgac aacggtgaaa tggcccggct
     6061 gcccgatctt gtaccctttg cccaaaagca tggcctgcgt attttgaccg tggccgattt
     6121 ggtggcgtgg cggcgcgcgc gcgagcggct gattaggcgc gacacagtcg tcaaactgcc
     6181 caccgacttt ggcgagttca tgctgtacct gtacgtggat gaaacctcca acgaacacca
     6241 cgtggcactt gtgtatggcg agcccgccaa acaagacgcc cccttggtgc gtgtgcattc
     6301 ggaatgcctc acgggggatg tttttgggtc gcaccgttgc gactgcggcg accaactgca
     6361 cgccgccatg cgccaagtgg ttgcggccgg ccatggcgcc atcctgtata tgcgccaaga
     6421 aggccgcggc attggcttgg tggccaaaat gcaggcctac gccctgcaag aacagggcta
     6481 tgacaccatc gaggcaaacc tgaagctggg gttcccggcg gacttgcgcg actacggcgt
     6541 gggcgcccaa atgttgcttg atttgggcat cactcgtcta cgcttgttga ccaacaaccc
     6601 gcgcaaaatt gtgggtatcc aaggctatgg aatcgaaatt gtcgagcggg tgcccatcgc
     6661 gttcccgccc aacgaggaca acgccaagta tctcaaaacc aagaaggata aaatgggtca
     6721 catgatctga cctggaaagg gagccatgaa ctataaggaa tatagcgggc aactcaatgc
     6781 caaaggcctg aaaatcggaa ttgtaattag ccggttcaat gacttattca ccggccaatt
     6841 gctcaagggc gccttggact gcctcgcgcg ccacggagcc gacgaaaaga atgtcgaggt
     6901 ggtgtgggtg cccggcgcgt tcgaacaacc gctcgcggta aaaaaactcg cgcaaaccaa
     6961 gaagtttaac gcggtactgg cattgggcgc catcattcag ggcgcgacgc cccacgccgg
     7021 cctgatcggc actcaagtgg ctcgcgcatt gacccaaatt agtctggaaa gcggcgtgcc
     7081 ggtggtggat ggcattgtga cggctgaaaa tctcgaccaa gccattgaac gctgcggcag
     7141 caagtcaggc aaccgcggct ggttggccgc ccagaccgcc attgaaatgg cgtcgttgtt
     7201 tcaacaattg ccctagtcag tcgcagtttt gtttgtcgtg ggtgcgtcac ccgcaaagta
     7261 gaaaggatgc ctcatgggaa gtcgccacga ggcccgtgaa tgggcattgc agttcctgtt
     7321 tcaatcggag ttcaaccggg aagaatcgct cgcggaaggg ttcaaactgt tctgggaaca
     7381 cttgaatgag cccccggcag gcacgggcgg ccaaccggcg gccaaagttg acaaacaacg
     7441 aaccaaggcg cgcgagttcg ccgaagaatt ggcgcgtggg gtcattgctc atcaccagga
     7501 aattgacccg ctgatcgcaa aatacgcgga gaactgggaa atctcccgga tgggaaccgt
     7561 ggatcgcaat gccatgcgca ttgcggttta tgaaatgctg tatcggcacg atatcccccc
     7621 ggtggtgtcc atcaacgagg ctgtcgaatt ggcgaaggcc tatagctccg ttgagtctgg
     7681 aaaatttgtg aacggcattt tggaccgtat ccgcaaaggc cttgaccgtc ccgcgcgcga
     7741 tgccaaccgt ggttcaacac ctccacaagg ttagccgaga tgggctcttg gttcgatagt
     7801 ttgcgaaaaa cccgcgcacg atttgccgat gcgctggccg gcattttctc ttccacacgc
     7861 gccgatcctg cctcaattga agaactgacc gatttactca tcaccgcgga tgttcccatg
     7921 cggttggtca cggaattgac ccacgagttg gaacgaacct cctcccgtaa agaacctctg
     7981 ctggcgacat ttgagcgggt gctagtcact gccttgggag agaatcccca gtggagttgg
     8041 tccacacaac ctaaacctgt tgtaatttta ttggttggca tcaatggttc cggcaagacc
     8101 acaacggctg ccaaacttgc ccgtcaagca caacaggctc atcgcaaccc attgctgggc
     8161 gccgcagata ccttccgggc ggcgggttct catcaactga agttgtgggc cgatcgtgtt
     8221 ggctgcgaat cggtaattgg cgctaccggc gccgatgctg ccgccgtggc ctatgatgcc
     8281 attgacgccg ccgtggcccg ccactgcgac acaattattg tggacaccgc aggacggatg
     8341 cacaccaaag agcccttgat gcgcgagttg caaaaagtgc gcgccgccat ggccaaacgc
     8401 ctgccggagg caccgcacca cacatgggtg gttctggatg ccatgctggg gcaaaatgcc
     8461 gtggtgcagg cccggcaatt tcatgaaatc acccctttgt ccggagtgat tgtgaccaag
     8521 ctggacggaa gttcaaaagc aggttttttg tttgctgtcc gccaagaatt gaatgtgccc
     8581 attctctttg ctggtctcgg cgaaggcatg gatgatttgg cgtcattcga tgccacacaa
     8641 ttcgttcgcg ctttgctgca aacagagggt gccacgtgaa cttggcctcc gatcaagttt
     8701 ggatgcaaca agcgctggcg atggcgcgcc ggggacttgg cttgacccgc cccaacccgc
     8761 cggtgggcgc ggtaatcgtc aaaaatggcc gcaagatcgc acagggttat catcgtcgcg
     8821 cgggggggcc gcacgcggaa atcgtggctt tgcgacaagc cggttcggct gcgcgtggcg
     8881 ccactcttta tgtgactctt gagccttgct caacccatgg gcgtaccccc ccctgcacgg
     8941 aggctatttt gcgggccggt attgcacgcg tagtggccgc caccactgac cctaatccca
     9001 aacacgcggg gcgtgggttg cgtttgctgc gcaaggctgg tctggaagtg cggcttggtg
     9061 tttgtcgtgt tgaagcaaac accctcattg ctccttttgc ctgtcacatg ctgcaccacc
     9121 gcccattttt caccctaaag ctgggccttt cagtagatgg acggatcgcc gaccacaccc
     9181 attcatcgcg ctggatcacc ggcccggcag cccgcaagga agttcaagcc ctgcgccgtg
     9241 cggctgatgc catcatggtg ggcgcgggaa ccgttcggca tgataatccc tctttgtggc
     9301 cacgtcccga tggccagcgc aacccatggc gaatcattat cgctggcaag ggccccctcc
     9361 cccttgacgc ccaggttttc aacgatgagc atgccaaacg tacgatagtt gctgctcccc
     9421 gcggttggca gccgtcatgc gcccaaataa ttcgcggtaa aggggccgct gttttagagt
     9481 tgccagccaa gagtcttctt gcctccttgg ctcgggaact cggcaatctt ggaatcatgc
     9541 atgtgttgtg cgaaggtggc gggattcttg ctggtgatct ccttcaggct ggcctggtgg
     9601 acgaactctg cattttcttt tcccccgtgc tcattggcgg ccccgttggc gcaatgggcc
     9661 tggctcaatg gaaattaaat gtggcccccc gcttttcgct ccgggaaagc cgacgcgttg
     9721 gccacgatct ttttatccga atgaccccag ccacagcgga gcgataaatg tttaccggac
     9781 ttattcaaca aatcggacgt ttgaccgaga taaccttcca aggcgatgcg gggcggcttt
     9841 cattaatggc caagtttaca gctcccttgc agattggcga gagcattgcc atcaatggcg
     9901 cttgcctgac tctggtggcg cagcaggaag acctcctcac ttttgatgta ttgcgtgaga
     9961 catttgaaaa gacgggccta cgcgggaagc ccaagggggc cccgctcaac ttggaacggg
    10021 cattgcgaat gggcgatcct ctcggtggcc atctggtgag cggccacgtt gacggcattg
    10081 gcctgcttca acgcatcacg gcagcgggtc gcgacaaggt tctgcaggtt cacgcacccg
    10141 ccttgatcaa tgagatggta cccaaaggct caattgccat tgatggagtc agtctcaccc
    10201 ttgtggaagt acgctcggct gatggcgttt ttacggttca cgtcattccc catacttggg
    10261 ataataccgc ctttaacagc ctgcgccccg gcgatcaggt gaacttggaa accgacatgc
    10321 tgggcaagta cgtccggcaa gcgctgttca acgccgccca aaattccgcg gtgtcatggg
    10381 ataaattgcg ttccgccggc tttctaccct gatcagccca tctacaagct gatttcttca
    10441 ttaagtcccc cgccaagctc caacgcaaaa cagccgagaa cgaagttttc aaactgtggg
    10501 ggcgcgataa ggaaaatccc ccattgttga atttgttcca tctgcaattc ccgcccgctc
    10561 ggcgtcattc gcaactctgc actggggatc acaatttggt tccaaccctg cgtgatggcc
    10621 atttctcgtt gaaatctgtc cgcatacggc ggattgccac gttgatcatc cacccgaata
    10681 gcgcaaatag tcggcatggc gtttggccag taaatgctgc cccgcaaggc ggtaatacct
    10741 cgccagtcac aatggaaaac tggccgaaat aacccggggt attcgacctc ttcccccgat
    10801 gagttggccc taaaacagaa ttttgagaaa tccagcggct gaacctggcc accttgttgt
    10861 gtccacccct gtctggccca gatagattga gggtccaaca aaacaggaaa tgcacgcgcc
    10921 tcttgggcca acagtgtgcc gatccataga ggggggataa gcgcaagtcc taggagtacc
    10981 gctacgcgca gcttcgagtg caacttcgct gtgccgatcg cgcctgttgc gcctaacagc
    11041 ccataaaacc aatcggacca ttccgcagat cggccaattt tcagttgcca catttcaatc
    11101 aggccagcca gaatcgcaag gccaaaccac aatttcaagc ccctaagtct tgcgggagcg
    11161 cattgggcaa gttgccacgc caagactgtg aacaaaatga catggctggt cccgcttacc
    11221 atacgccaca agggactgtc atccgaatgc ggcaacggca caacaagagg tgcaaccatg
    11281 accacgctga taagccatgg caaccacctg ccccccgggt gattgggcat ggttattcct
    11341 cgtaaaccat tattcgatac caccgggtgc ggcggagata ggacgtgcgg tcttcaaacg
    11401 tatacttcaa ttgcgaacca ccactgggga tcacaaagtt gctacggttg atttgtgccg
    11461 ggccattgta cttctcatcc caggtcaccc atgtctgccc gaaatcatca gaatattcga
    11521 ccacgtatcg tcgtccctgc accccaccct gccaatcaaa acgaatgcgg ccagaagatg
    11581 ggtagacttt caaatacgcc ggcggcaacc aacgcaccaa gaaacaggtg tcgctcatat
    11641 ccactatgtt ggtgttgctt tggctgatta cacgccagcg gcctccggcc gtctgatact
    11701 cgattcctgc ccgccagata taatattcct gataggcatt aacgttggtc agggtggtcc
    11761 aggtttctcc gcaatcgccc gaccattgga cggacaccat ttcattggta tcaagcaaat
    11821 gagcagacca gacccatggc cacacttgat cttcttgtgt taccatcagg ggttgctcat
    11881 tcatcgtccg ggcatacaat tcgatatcaa ttgaaccttt gctcgcctgt actgtatttc
    11941 catacattcc tatattgatg cgttgtccat tgtcctcagg ctcttggctg taatctgcgg
    12001 caggattgcc atgatccacg gcccaagaag ttatgccgtc ggttgtccag ccggagtccg
    12061 tccagcgacc atattccgac aatggatgga aatcgcctaa atccaaattg gccagatcgg
    12121 cattggtgat ggcgctgcgg aagtcgcgat tcatttccaa ctgccaccgg cgcagatcat
    12181 tggttgcgcc agcaaagaac ccgctgccgg attcttcgtc accaaagtca tacaagttgt
    12241 aatccaagat tgcttgtgcc agcagtgacg tagccgaacc gattgcatag gcataggatc
    12301 caggagcggg aataaagata ttattccgca gggtgacaac ttcggtgttg cccagttgaa
    12361 tccccgccag agacgtattg acgaatgtga ggttttccaa aatggatgtg cgggtgttgc
    12421 tcaagctaat gcccatttcg gcattccaaa aaccagaatt acgaatgtcc gtatttcttg
    12481 tccccagcgc ctgaatacca atgccaacat catgcaggac aaggccggat gccctgaccc
    12541 ccacattgga ttccaacccg atggccctgt tgacgccgct gattgaaaaa tctgtcagcc
    12601 ggatgtgcga tgccttcaca tccagaccca tcgctgggta gttcgtctgc cccaatcgca
    12661 cgagttgggt ggcatagggg tcattcgtgt tgccacggat cacaacatcg gcagtttggt
    12721 tgccactacg cgaccagatt accttcacat cggatgtgct ggcataggtt ccggtgtcaa
    12781 catagaccgt atcgccgcct tccaagtcat aggcatccag aatggcctgc aatgttgctt
    12841 tcggcgccga gttgctcaag ccgctgttgg aatcactgcc tactgccgtg cagtagagat
    12901 cacccaacaa gctggagtcg tttacaaaga acttatgagt gtgattacgc aactggaagg
    12961 gatgatcggt ttgatccgat gtcccggagg catcctcggc gacaacgcgc caataagcgg
    13021 caaagctatc cgggaaacca gtcgtgtccc acggatatga ttcatcagtt gcgtttaccc
    13081 ccgtagcaat ggtgtgccaa gcactgccat catagtattc caaagagacg gtcttgccac
    13141 tggcattgcc ggccaaccag cgcagggtca cgttgcttcc cttcaatact ccgccatcgt
    13201 tttgggtcac ggcagtcagc cagaaattcg taatactctt gctggcctca agagtacgtc
    13261 cataggcgcc aaggttgggg cggtagccat ggggaccgat ttcgtcgcct gttccgattt
    13321 gcggattgcc tgcgtcaatg gccggcgagt gctgtgggtc aatgtcccaa tccttaaaaa
    13381 tcgggctcca ccgacccatc ttggaattca agtggaagtc gccagcagct tcattttgga
    13441 atagtggctc aatggccaag ctgtttgcat cgcggcccga agcggcctgc caataaccca
    13501 gtgtttccca tcggccctcc ttgatcccca cccaggcaga atcgcgagcc acaaagttgt
    13561 tccagtctga aatcaatccg ccatcgtgcc aggccaacac gctattgctg tcacttccgc
    13621 cagcgacgag aatattgtgc cccaaccgaa gggttcctgc acccagttta cctacggccg
    13681 taccgcgagt cgcaaccact gtcgaattgg agacagtagc cccaagcgca tttacaacca
    13741 aggcagtggt caagttattt gtcgtgaaca cgaccgaatt gagcaaggta atattggtgc
    13801 ccaccagtga ggcttgtccc caacgctgct ccatgcgttc gattcggtta ttgcttcctg
    13861 aaaacgataa gttgccccga tcaagctgca gctttttgaa tacagcattt tcgccgttaa
    13921 tcgtcaccgt gccgttggtc acccagagat tactggcagt aatggagtca cccaaaaatc
    13981 gaaccgattc gccaacgaag cccaagtcgc ggatatctat gtgattggct tccatgaaca
    14041 tgaatgcccc agcagtaaat cgattggtgg ccacgatcca actccccgca ggattagtac
    14101 tcccgcgaac aatgacgggg gtattggtcg tgccaccgtc ggacacctgc caaataattg
    14161 gacgattggt gtcggtcatg taatggattc ccgtgtcaat caaaaccata tcccccggtt
    14221 cgatatcaat atcctccagc agcgcattaa cggtcagctt gggtgcccga gcgtaatatc
    14281 cgggattagt gtcgctgcca atcgcagtcg tatagacatc gttgaccgtg ctcaaatcat
    14341 tcacgtaata cttgaagggg ctgtttcgga ggccaaacgg ccggtcggtt gcgtcccaca
    14401 cattggtgtc attgagcagg aaaattcgcc aatactctgc cgggctcgac gtccagcgct
    14461 cggttccatc cacgttgcgg aggtcgctct gccaatagaa tgcaccatcg cttacggtag
    14521 cctcgccgat tttcaaccaa ttgccatcgt acgaatattc caactgaact ttggtattgg
    14581 tcggaattaa ggaactatag ccccaagtaa gtacagtatc accaaaaagg ataccgcccg
    14641 acattgctgt aatcgcttgc agccagcgat tagtattgct cttgctcgcc tctgccgtgt
    14701 tgccatagag gccgatattc cgcctggtgc cgttcggtgc cggctcgctc gtccagcccg
    14761 tagtcggtgc ccccatgtca atcaaaggag aaaatactgg ttggccatta actggcggat
    14821 cctgcaccca tcccacgcca agctgataac gaccggcaga gctcttaaga tggaagtcgc
    14881 cagttgccgc gtcggcaaac aaaggatcgg tgtgcaagga atagcggtca ataggcgcct
    14941 tggccctgac ccactgaggt aaagattcat actgcacgcc atcaatcgaa gccatttggg
    15001 ccccattggt gagaaaatag gcgttataat tagcctggat agtgatgttc gttggcgact
    15061 cataacaata gctcgccagt ccgaaagcac tcactacgga attggtgatc cccaaatcag
    15121 cgcccgagcc caaagagacg gcagatgctg cattggacca caaaacgcaa ctttcgatca
    15181 tcgccttgcc ttgagcgaga gaaatcgcac ttgccgtgcc atggcgaatg gatacacggc
    15241 gcagggtaat atcctgcgat ttattaagca ccacgccgct tccaaatgat tcttggatat
    15301 ccaaagactc aagaacacag cgcgaagatt gttccgcaac agatacgccg ttggtgaacc
    15361 ctttgattcg aatgttggcc aagcgtacat catgtgcccc atagaattca aacgccggag
    15421 cacgcatgtt aatcccggct tgcaccagag tcccgcctgc agccagattg gtgctgccaa
    15481 gtatcagcac ttgattggtg ccgctgccgc tagctttggg cgaaatgaat acttggttcg
    15541 ttagggtata cgaaccggta tctactcgaa tactgtcgcc acccaccagg cgatattgtt
    15601 cgagaatgct ttggatcgta tcgcgagggc tatccgcgcg gtaacccaaa tttgtcttgg
    15661 caccgggagc tgttgtgtaa atgtcacccg tcgtgtcggt atcgtttaca taataaatca
    15721 acggggtcgg tctgaaggta aagggcaccg ttgttgtccc gataatgttt gtatcagtca
    15781 tcaggaaaat ccgccacagg gccagtggcg acggcgggtc tgcgctatac cacatatatg
    15841 gttgtgatcc aaccggaata ccattggcca caaagagcca cgtcgctccg ccatcagggg
    15901 aatattcaat ctgcactaaa ttggtgggac taatccctcg tgacaaccaa tacaagggct
    15961 ggccgtatgg cgccacgccg ccgtccttca gggtgatggg taggagcgcg gggtggttgg
    16021 tgtcactccg tgacgcttgt gcggtgccac cgtaggcacc taaattgatt ctaccaccat
    16081 taggtgccgg ttcatttgta aagctggaag ttggattgcc cgcatcaata gcccaagaag
    16141 tgttcgtgga gattgcccaa gttccgttgc tataatatcc cccggtgctt tgcaggtgga
    16201 aatcaccagc agcaggattc acgaaaagag gatcaatcac cacgctgtgg gtgtctttgt
    16261 tttttgtctg ccactccgac aggtttttat aatctatttt ttcggataaa ttggaacaca
    16321 acaccccatt cggcgggaag aaatacagat taaaatcgcc gccgatgctg gctgactgtg
    16381 cgctctctac gaagatcgca ctagcattag ttgtaaccac aatactattt acaagagtca
    16441 aacctttatc agtgcttatt ccgcctcccc gattcccata aattgtcgaa ttgattatcg
    16501 tttcgccgcc cttgctccct gcgctggcat aggcactgcc accattccgc catattcgcg
    16561 cattttgtat cgtgatgttc gccgaaccac tgacataaag cccgttggtt tgattatccc
    16621 aaagttccat gccaatcagg acaacattgt cggcattatt aattgagatg ccgtgctgcg
    16681 cctgcgtgac gcggatatcc tgaaggcgga tattgcgtgt attttgaata gcgaatagct
    16741 tgttctcgac accttgaaaa actgccccgc ccgcactaat atttgtgctc ccataaattc
    16801 gtacaggcat ggaagtcgtc ccttgatggc gctcgttgat caaaatcggt ttcgataagg
    16861 gatatgtgcc ggtgtctaca taaattatgt cacctggtcc gactggatag ttcgccagta
    16921 aatcagccaa gctattcaag ggttgtgcgg catttgtgcc atacggtgtc cctgcactaa
    16981 atggcaagcc gggccctgag caccatacat cgccgtttgt agaattgtcg ttgatgtaaa
    17041 aggttttcgt tcctggcggg ttgtggggaa tgggcacatc cgtgacatcc cagatattgg
    17101 tgttgccttg gtacatgact cgccattgca ttcccaaact taggggcagc ccacttaaat
    17161 cccaaagaaa ctcgcgtgtg gcagcgggaa cattactggc gattggcttc cacgtaactt
    17221 catagtctgg agaataatcc aatcggacca gcgcgccgtc cggcaatcca ccaaatgtcc
    17281 agtacaacag agcttcaccc gataagattc cttctgcatt gtaggagatg gcctgaaccc
    17341 atggcggatt ggtttggctc atggatgcct gtgccgtatt gccgtaggcg ccaatgttaa
    17401 tcattccacc atttggctcc ggctcacgat ccgcagcata atctggcgcc ccagcatcaa
    17461 tcagtggtga acgaacggca tcattcgtcc aggtttgtga cgcggggata aagcgacctt
    17521 tggtgctctt ggggtggaaa tcaccggtta tttcgtcggc aaaggcaggt tccaagctgt
    17581 acgtggctgt catcgaatgg cgatcagagc cgactctctt gctccaagtt ggcagatcat
    17641 taaaatagtc actgccaccc acttgcaggg cttgttccgc aatgagcgcc ccattccacc
    17701 gggcatagct attgtaatcc cccctgaacc caccgccacc aatactggat gaaatcccgt
    17761 agttataaat acgcccattg ggtccggagg cataaagcac actattggta atctgcaatg
    17821 aaccgctacc gacagtaatc cccgaataac tgccccatag cacactgttt tcaacacaca
    17881 gatccccctt gttaagagca atagctatcc cccccgtggg gctggcattt ttccatagca
    17941 ccgagcgaat catccgagtc tcttcacagt cgtcaatagt caatccggtg atacggttat
    18001 tttcgacgcg cacaccatcc aattccagtc caatggtttt atatgcggat atcccagtct
    18061 gtgcattacg gatggtcagg tctttcaggc ggatattaac cgcgtagtcc aaagcaaaga
    18121 cctgcggagc tgaaaacgat ggcgcgataa agatggtcgg ggctacaggg ttggtactgc
    18181 cctggacggt aatgaaataa ttggttccat tcgcgctatg cccggagtct ttttcattaa
    18241 tctttatcgg agggcttcct gctaggtagg taccggcatc aacatagatg acatcggatg
    18301 ccgctaaatc ataagaatca aagatggcct gcaaactggc ttttggtgtt tctggtgtca
    18361 gcccatcgtt gatatcatcg ccaatggcgg tgcaatacac atcccccgtc atatttgtat
    18421 cgtttacgta aaatggaatc atgccgccat tcttgagcat gaaaggcccc gcttgatctc
    18481 gaatggaata gtcatccagt gacgtaatac gccaccacgc ccgtgcggaa cggccataag
    18541 gcacgctgtt ccacttatag gagccggatt ctgcgggcca accactgaca atattggacc
    18601 aagttacgcc tgcatcagat gaatactcca aaagcacgct gtggttggta gcagccccaa
    18661 ttgctgacca ttgtaaaacg atgtcttttt cgacttgtcc cccttctgtg gggaacacac
    18721 aggttaatat cccattggaa ggtgtcttac tagcttcggt ggtacccccg taaaggccga
    18781 tatttacccg ggtgccattg gggagaggct ccacggtcca acccaaggac cttgggtctc
    18841 cagcatcaat catcggtgat gataccggat cggtcaccca tcccgtcccg ggctggtagc
    18901 gaccgcccgc actctttaag tgaaaatcgt aacccggttc agccaataag gggtcttgcg
    18961 ccaatgaatg cgtgtcagca cccaaatccg cagcccattt cgagaggaag gcatagacat
    19021 tggtccgcgc gctgctgctc gcaccagtct gcaaagcgcc tatggcggca tttggatggc
    19081 caacaaacag gttgttgtag tcaccagaat agccggtcag tggtttatct gcgtgccaat
    19141 agataccata agaattgggc ctggttatcc cgacaatgct gttgctgaca tgcgcataac
    19201 cttgcgccaa atagcaccct gcccgattgc cccagaatac gctggattgt atatccaccg
    19261 ttcccatctc gactgctgcc acataaacac cggcgttttg attgccaaca aacgttgagt
    19321 gacggacctg catgcttgat gaatcaacgg acgaaatacc attcgcacaa ttacgcattt
    19381 ccagccattc gacagcaatc atccggctat cttgcgcgta tatcccgttc gatgcgttga
    19441 gaattccaag atgcgccaat cggatgccat aagtgttttt caactcaaac gcattgacgg
    19501 ttggatctgt catgacgaat agactgcccc cagccaaggc atttgtactg ccaatcacat
    19561 taacctgacg atcggcggcc tgaataaaat ctcctgcgtc caaatcgcca aacacggttg
    19621 gttgtgacat actatatgtg ccggtatcaa catatatggt atcacccggc tccaggttat
    19681 agctgtcaat tatctccgcc acccaccgtt tggggctatc cggagaaatg ccggtattcg
    19741 ttgagttgcc taattcggtg cagtagacat cgccatcagc gtgatcgtca ttaacgtaat
    19801 aggtaatagc gctgtgatga atcacaaaag gccgcgcact gattgccgtc accatcggct
    19861 catccagatc cgtaactttc caccggccaa gtggcgagga tgccaaactt gagctgttcc
    19921 atttaacacc gccgagtgtg gctgacacac tagggactac caaatgccaa gtctccccgt
    19981 cgtcatttga gacctccacc cgcaccgtgt gcccggtcgc catcccagta gctaaccaat
    20041 tcaaggcaac aggcccggta acaacaccac cgtgattaag cgtaagtaaa cataaagctg
    20101 aatttgtttc agactttgat gccaaagagg tcccgccata aaggccaaca ttaagtcgct
    20161 gtccattggg tgcgggctca ttggtccacg tttttgcgct tggctcaccc atatcaatca
    20221 gcggtgaagt ttgcgcatca ttggtccatg acccggcaat cggattccag cggccagccc
    20281 ggctctttaa atggaagatt cgattcgacg gattaacgag gagtggatcg ccatcatagg
    20341 agtgaaggtc attactagaa ctggtagccc aagcgccaac ggtctcatag accaaaggat
    20401 aacggctccg atcccctccc tgcatccgcc gcgccacccg tccatgtgtg ccgagccata
    20461 aagcgttata atctgccacc aaattggctg tatccgccaa atcgtatatg taattttccc
    20521 cggtaatgtt catgatgtta ttcattacag tggcccggtg tagttcagag attttaattg
    20581 cgctctggcc aagattccac aacacgttat tatttatgac cggaaatact gtattagtag
    20641 atctgtccca attgtctaca accactccag tgttggcatc caccactacg caatgttcca
    20701 cgcgcaagtt gtttcccgag ttcaagcgca caccgacgtc gccccccccc accgcgcacc
    20761 attccaaagc aacatcccgc gcctgattga gagcaaaggc ggttcgtgtg gtgttggaaa
    20821 ccacgatatt gcgtaaaacc accccaatga cataatctgc ctgcagcccc acactattgt
    20881 aatttcgctg cagtaccgtg ccgcccatca atgaagacgt acttccttgg aaaacaacgg
    20941 gagggacgtt taacgcaccc gcggagtcgg cctgggtgat tcgccacgga tccacacccg
    21001 tggcataaac tccggtgtca atgtaaacaa catcaccact ttccagatcg tatttggcca
    21061 agagtgcatt gagatcagcc attggcgcat tgctcgatag cccgctattt gcgccctggc
    21121 cgatggccgt acaatacaca tcaccgatcg tgctgccgtc attgacataa aaatagaacg
    21181 gtgcattgcg cacggagaat accttgtcac tttgggtact gctgccatca ctaccttcaa
    21241 ttttaagttt ggcctgaacc gattgttcgc tcgccgttga gtcccagttc cacacgccag
    21301 agttggccgc aagcccattg gttaacaatg accatgttcc ccctccatcc gctgaatagt
    21361 aaatggaaat ggtgccgtta gtgatctgac cccgggcatt ccattgaatc ggcacgttgt
    21421 tgccagaagc gcgcccgcca tcattgaacg taatgagtgt caaggcgcta ttggttgggg
    21481 ttttggaagc ttgtgccgtg ttgccataac gcccgatgtt aactcgtgcc ccattcggct
    21541 cgggttctgt gcaggcgagg ctcggcgtgc cggcatcaat caggggtgag gtcacagcat
    21601 catttgtcca ttgatttgcg gcggggaccc agcggccagc ggagctcatt aagtggaaat
    21661 cgccggcggc ggcatcaaca aacagtgggg agtgcgacaa agaatgtgaa tcataaaacc
    21721 cattggtgcc cacccaactc ccgacagaat catattcgcg agcaaagcca cctgtatagg
    21781 aaagtgccgc caaaccacca ttgcccaagt aaatattgtt gtagtcagaa accagggttc
    21841 catttaacag ataagccgga actcgaattc cgaaagaacc aataatacaa ttgctgatcg
    21901 ttgtgtaagc gtcattgtaa gctgagtttg ccccgccttg ttgcaactga ttatttacga
    21961 caacctgagc ggacacattg gaccacacga cgccaaattc caatgttgtc ccgtaagagg
    22021 cctcgcaaac cacgccattc gtttgcgccc cagtgacagc aaagttccgt accagaatat
    22081 tggagctttg tgagatcgta attccgctac ctacattgcc caatatatca acattctgca
    22141 ggctgatatc ctcagaggcg gaaaccattg cggcacccgc catcatgttt tcaaatcgta
    22201 aatgttccag cttaagtccg cgtgtattca ccaagttcag gccattttgg gtaaagacag
    22261 accccgcagc gttggtacta ccccgaatca ccacgggctc ggctatttca ccgcggtcca
    22321 tatatccaat gatgaccggg gtggagttgg tatatatccc cgtatcaatg agaatcaagt
    22381 ccccaggcct aacgtcgtaa gttgtcaaga ctgctgccaa ggaaggcaag ggtgatgacg
    22441 gatgcaaccc cgaattggtt atcgcaccaa tcgcggttgt atagatatcg ccattggttg
    22501 ccgcgtcatt gacatagaaa gtccaagggg cattgcgcac catgaaatct ctttctgatg
    22561 cggtcaccca cgtactttga ctttgacttt ggacacgcca acgacaggcc gtgagcggag
    22621 gcaagctggt cgtgtcccat tcatggaaac cgaccaaccc agtgctgacg ccgatattct
    22681 gccacgatgc gccactattg gttgaaagtt gtacattcac acgatggctg ggattggtta
    22741 ccgccccgcg ggcgatccag cgtagtcttt gagtgccgct ggccgtaccc ccttgattga
    22801 gcgttaagag cacaaaactc ccattggtag gcgaccgact tgccgcagga gtcccgccat
    22861 atatgccaat attggcccgg ctgccattgg gctctccttc ggcggcccaa ggcaaggcgg
    22921 gatctgcgga atcaatcagc ggcgacgttt ccgcatccag cacatagtcc tgagtcgtgg
    22981 gatcgtagcg tccagcctga ctgcgtggat acaaattgcc tgtcaccaaa ttagcaaata
    23041 acgggtcggc cataaatgag ttcgattggc cttgtgtcca ttcctgccat tcagtcagat
    23101 cacgaaactg aatagtttgc acgccaaata cgctccctcc ccgaggagtc ttggcaaaaa
    23161 tagtattggc atggggctga tgaaagagat tgtagtcacc cgccacggtt ccggcgccaa
    23221 gtcggaaaat ttcgtgagcg gggctgttgg caatcaaggc gctgttgcta acatataagc
    23281 agccggactc gactccgatc aacgtgcctg ttgtcaacgc aatcccattg ctggcaggca
    23341 acggtgacaa cacgccctga ctccagaagt ttgtgaccgg cgcaatttgc tgggacataa
    23401 tcggagccat ataaaacccg gtttgtgaaa aattcagcgc cgcacagcgg atgaattcgt
    23461 tctccccgga ctgtacactg atgacaaatg cattgaccgc cgcattcatg gaacggaccc
    23521 caacaaaccg atttgagctt gaccggttca gaaccaagcc ttgactcccg ccatgcaggc
    23581 ggatatcgcg cagttcgata ttgcttgaat tcaaaatttc caagattgtg ccggatgcat
    23641 ttgtgaagac agttcctccc gccgcctcgt ttgtactgcc ttgaatgatc aggcggttgg
    23701 ttggggctat cgccgagatg gcaatggtca atggtgcggt gcgtgggtac acaccagtat
    23761 caacatacac cgtgtcgcca tgttccactt tgtagcggct gagcaaggtt gccagcgagt
    23821 tcaacggcgc tgccgcgctt aaacccgaat tggtcgcacg tcccgggccg gtgcaataca
    23881 cgtccccgac agtgctgttg tcgttaacat aatacgctac cgggtcattt ttgatggcga
    23941 agagcgtctc ggtttgtcca cacacggaac tattggttgt gcatacgcgc caaacccctt
    24001 gcgcagtact taggtaattg gtggttgccc agggtaagcc ggttccaccc atgtcggcat
    24061 acacattggt ggtaataatg gaccacgtca ccccca
//
LOCUS       DAOPNP010000026        24049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2637_length_24049_cov_8.988289, whole genome shotgun sequence.
ACCESSION   DAOPNP010000026 DAOPNP010000000
VERSION     DAOPNP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24049
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_24049_cov_8.988289"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1170)
                     /gene="recA"
                     /locus_tag="PKI64_03745"
     CDS             complement(91..1170)
                     /gene="recA"
                     /locus_tag="PKI64_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOE00157.1"
                     /translation="MPPKTPAAAKKDANSNLPPALSAVLAQIQKEFGEGAIMRLGTEG
                     AVARIPAISTSALTLDIALGIGGVPRGRIIEIFGPESSGKTTLVLHIIAEAQKNGGMC
                     AFIDTEHALDPHYAQKIGVDIDNLLVSQPDSGEEALTIADQLIRSNALDVIVVDSVAA
                     LAPRAELEGQIGDSHVGLQARLMSQAMRKMTGILDKSKTCCIFTNQIREKIGVMFGNP
                     ETTPGGRALKFYSSVRLDIRRIAAIKAGDGKVVGNRTRVKVVKNKVAPPFTEAEFDIL
                     YAEGISKEGAVIDAANDLGLLDKRGTWLSYDGQQIGQGRDAARENLRNDPALFAKIRD
                     AVIAKHAEAGVKPTAKEKAAAKAEE"
     gene            complement(1325..2389)
                     /gene="rfbB"
                     /locus_tag="PKI64_03750"
     CDS             complement(1325..2389)
                     /gene="rfbB"
                     /locus_tag="PKI64_03750"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOE00158.1"
                     /translation="MPRRFENLLVTGGAGFIGVNFIRWLFTQPDCPRRIINVDKLTYA
                     GNPLSLLDLEKSVGERYVFRKADICDAAAMKEVIETYAVDAIAHFAAESHVDRSIVAP
                     DDFVRTNIIGTYTLLQAAREHAAQIKLFHHVSTDEVFGSLGPAGFFTEETPYAPNSPY
                     SASKAASDHLVRAYHETYGLPTTLSNCSNNYGPYQFPEKLIPLMILNASEGKPLPVYG
                     DGMNVRDWLFVTDHCAAIWQVMQHAAPGATYNVGGRNELPNLRVVEKICDLVDAKAPP
                     LPNGQPRRSLITFVKDRPGHDRRYAIDCAKIERDLGWQPAETWETGFARTVDWYLQNT
                     EWCAQVRSGEYQKWIATHYG"
     gene            complement(2392..3267)
                     /gene="rfbD"
                     /locus_tag="PKI64_03755"
     CDS             complement(2392..3267)
                     /gene="rfbD"
                     /locus_tag="PKI64_03755"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOE00159.1"
                     /translation="MKILVTGGMGQLGWDCREVFTGHEVLALDLPNLDITEAASVDGI
                     LSLWRPDVIVNCAAYTAVDRAETDVAKTWNVNREGPALLATRAKAYDIFLLHVSTDYV
                     FDGARPVPEPYLESDQPHPVSVYGKSKLAGEREIRERAPRHAILRTAWLYGAHGYNFL
                     KTILRRAVTDPRRGLRIVNDQFGTPTWSRRLAEQILAVIEADATGLFHASGEGHCSWY
                     DFAKEFISLMKVDCEVRPCTTADYPTPTVRPANSILENANLKKAGLNRMQDWRADLGE
                     YVQRYREALLNEVRA"
     gene            complement(3264..4172)
                     /locus_tag="PKI64_03760"
     CDS             complement(3264..4172)
                     /locus_tag="PKI64_03760"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOE00160.1"
                     /translation="MTWPTIREILAARRQPTLSLEFYPPKDPLSFGILGGSIERMRPV
                     RPDFVTCTYGAGGSSREFSFAAWELLARMGFQPVVAHLTCVGASRADLAAQVDRIHAA
                     GIRNIMALRGDPPRGETEFKPAPDGLAYAADLVALIKERHPDICCGVAGYPETHPEAD
                     SLVDDLRHLKTKVEAGADFITTQLFYDNAVYFQFVAAAREAGITVPILPGLMPVSSVA
                     QLERSLAFSRATAPAALRTALAAGSADDARAAGIAWLTEQIEGLVAGGVPGIHLYILN
                     QARTILDPRLAECLARWRRLPTGATT"
     gene            complement(4169..4951)
                     /locus_tag="PKI64_03765"
     CDS             complement(4169..4951)
                     /locus_tag="PKI64_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOE00161.1"
                     /translation="MSSALERVELLLGTDALERLAQARVMVAGLGAVGAMAAESLARS
                     GVGHLVLVDFDELRATNLNRHPFAAQSTLGQLKVHVAARFLRDIQPGLDLTPLPLFLD
                     ADTAARVLAEQPCDVLLDAIDSLHPKTELLLAARRAQVPFILTCLGAARRCEPARITS
                     ADLAASHTCPLGRLLRKRLAKHGGSAGIRCVFSTEKPTPPAAALPTADFYVRGRLRPP
                     LGSLHCVTATAGLRAAHEILTFLLAEKNGCRPPDASAILPEL"
     gene            complement(4983..5909)
                     /gene="era"
                     /locus_tag="PKI64_03770"
     CDS             complement(4983..5909)
                     /gene="era"
                     /locus_tag="PKI64_03770"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOE00162.1"
                     /translation="MNTSPFRAGLVAIVGRANTGKSSLLNALIGENVSVVSPVAQTTR
                     RPIRAIHNEDGLQIVFLDTPGIRKATHTLGSLLNRTARGLVTGADVILLLLDASRPPQ
                     EEDRGWMKKLATEMSPLFMALNKTDLGLNRAEHEAAWAETITAQQARDPAYNPPPVTW
                     FEVSAHTGAGLPALLAALREKMPAAEPLFPTDMLTDDPGPFFIADVIRAQINLRLKAE
                     LPHAIAVAVDHLAETDEGVTVQATIYVEKSSQRPIVIGARAKMIRDIRHAAQKALAEI
                     YGKPHKLELWVKVEPNWSRNYWLLKKFGYVGN"
     gene            complement(5906..7354)
                     /locus_tag="PKI64_03775"
     CDS             complement(5906..7354)
                     /locus_tag="PKI64_03775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE00163.1"
                     /translation="MNSAPTHSGGSYLKWHFLGGADTVTGSRHLLEYNGTRILFDCGL
                     FQGRRKESAEFNQTLCFDPEHVEAMVLSHAHIDHCGNIPTLVKAGYRRDIHATSATAD
                     LLPIMLRDSAHIQEADAAYLNQKTNRRGLPPIVPLYTMEDAEAALKLIRPHEYDDHLR
                     LPGDIVVTHVDAGHILGAALTLIQIPYNGGDFLRIALAFDLGRRNLPLLNDPVQLRDI
                     DILICESTYGDRLHDNALNAHEALRESIVRALARGGKVLIPTFALGRSQEIIYHLIQL
                     RERGLIPEVPVYVDSPMAHAISGVFEKHVAYLDNESQALRNHLGSIVSPPWLTFTQSV
                     DESKAITSSAQPAIVLAASGMCEHGRILHHLKHGIENERNLILLVGYQALNTLGRRIQ
                     NGEKRVRVFGDEFDVKAEVQTLNAFSAHADRLELLRYIRGAKPKHVFLVHGEPSQRAA
                     LAERVGRLPHTTVELPANGDVVDLSSYLRPTA"
     gene            complement(7403..8947)
                     /locus_tag="PKI64_03780"
     CDS             complement(7403..8947)
                     /locus_tag="PKI64_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOE00164.1"
                     /translation="MLAKVFSGAVYGVDAYEVEIEVNAGHGEPKVVVVGLPDAAVKES
                     SDRVWTALINSGFSPPMGRTTVNLAPADIKKEGPSFDLPIALGMLAAEEQMDGARLAQ
                     FCVTGELALSGEVRRVRGVLPIALQARAAGRQGILVPPENAEEAAVVEGLAVYPVATL
                     RAAADFLDGKKTIEPFRVDMQTAFGSGAAYDDDYADVKGQEFARRAIEVAVAGGHNIL
                     LIGPPGTGKSMLAKRVPTILPPLTLSEALETTKIHSIAGVLPAHQAVVSGRPFRSPHH
                     TISDAGLLGGGAHPLPGEVSLAHNGVLFLDELPEFHRNVLEVMRQPLEDGTVSIARAA
                     ASVTFPCRFMLVAAMNPCPCGHFGDATKNCRCTPIQMQRYRNRISGPLLDRIDIHVEV
                     PAMEYQQLSSLEKGESSAAIRERVVASRAIQRERFARSRGAHVIHCNAGMRARDVHKH
                     CVLDETAQGLLKAAMADLHLSARAYDRILKVARTIADLAAQEQIAADHIAEAIQYRSL
                     DRHYWT"
     gene            complement(8954..9160)
                     /locus_tag="PKI64_03785"
     CDS             complement(8954..9160)
                     /locus_tag="PKI64_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00165.1"
                     /translation="MYKTCPEKYALAWGVRPNTVDHNGWIKEQRYMDKAIDFAEFMKK
                     VTMAQRGEWGGQPFVKVKNIKTRD"
     gene            complement(9232..10050)
                     /locus_tag="PKI64_03790"
     CDS             complement(9232..10050)
                     /locus_tag="PKI64_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE00166.1"
                     /translation="MKKFLTTLLLVILLAAVGGGFLLLRFERQQLYHPRPDIHATPAH
                     FQQRFQEVQFTAADGTTLSGWWIPANHPRGTVVFCHGNGGNISDRAHLAPAFTKRGFN
                     LFLWDYRGYGQSAGSPSEPGLYRDARAAFDAAQPMSRNLPIIVYGHSLGAAVACQLAR
                     ERPVSGLIIEGGFASMADMARRRFPDWPLDRLLTVKYDAAATAAALPGIPKLFGHSPR
                     DEVVPFQSGRLLFAAAAAPKTFTILGGAHSDSSWLTPGQPGGEDLDNFLAQFKP"
     gene            complement(10056..11186)
                     /locus_tag="PKI64_03795"
     CDS             complement(10056..11186)
                     /locus_tag="PKI64_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOE00167.1"
                     /translation="MNKSTAPLIFVATGLDENDVRHATGMAASDPFGLLVEPGGRLHL
                     LVSPLEASRAKKTCPRAIIHTPASLASPGIPPPATLSQQFVALAAKLGVRRVTVTPHF
                     PIGVARALTAAGVAVEVQDAPPFPSRAIKTAREVAWITQAQEAAVAAMRAAIECIRAA
                     TIGPRKVLRHRGRILTAEHIKNVIAHALMEHQCADQGTIVAVGPQSARPHDTGSGPLR
                     AGVPIVLDIFPRHRETGYWGDITRTVVRGEATPQVRQMYLAVHTAQRDALAMLRPGVE
                     LAAVHQTVVDSFLRAGFITKLEPPGRECGFIHSTGHGVGLDIHEEPRLSRGPGQLLAG
                     HVVTVEPGLYYPGIGGVRIEDTVVITQRGYKLLATFPKKLEV"
     gene            11611..12984
                     /gene="dnaA"
                     /locus_tag="PKI64_03800"
     CDS             11611..12984
                     /gene="dnaA"
                     /locus_tag="PKI64_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOE00168.1"
                     /translation="MDEKKAKEVWAKACEHLAEAVSADIFSRWIGVIEAAGDDAPGQL
                     TLTVPNGFYQDWLEEHYLPMIRKALGAAGHEHIAIAFVVNDNGPAPAPATLVKSNEPA
                     PAKRRGPKIYNVGGTLNPRHTFENFVVGPANTFAHAAALAVVDAPARAYNPLFIHGGT
                     GLGKTHLIQAMGHALIKRGKGTVCYTTCEDFTNEFIQAVQQHELTSFRRKYRNIDLLL
                     IDDIHFLARKEGIQEEFFHTFNSLHQNQKQIVMTSDRPVSEIANLQNRLVSRFEWGMV
                     TEMEMVDLETRVAILRQKREELGIQLPDPTLFFIAEHVRSNIRKLEGSLLRVASYASL
                     TRKPLTTEALEYLLRDTIEQENQTAVTLEGIQRIVAEYYDIRLGDMTSKQRPNNIAFP
                     RQVAMYLCREMTEQSLPAIGNAFGRNHATVLHAHRSIGAKMKTDSSLRQTILSLQQRL
                     GGAAAPV"
     gene            13284..14378
                     /gene="dnaN"
                     /locus_tag="PKI64_03805"
     CDS             13284..14378
                     /gene="dnaN"
                     /locus_tag="PKI64_03805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOE00169.1"
                     /translation="MKIKVKKEAMLASLQKVQSVINPRNTLAVLANVLFRAQDGKLEL
                     TATDMALTLRTTMEAEVLQPGSTTIPAKRILGVFRELAVAEVELEVNDQNITLIHAGS
                     SHFKIHGISENEYPNLPELADAKTYTLDQGDFKNMLKLTSFAASLDTNRQLLNGVLLS
                     FKNGKLSVVATDSRRLAHVEQEVEFPADAALDLIVPLKTVDELLKTLGDSGPLKIMAT
                     PKQVGFDFNSVFIVSKLEEGKYPNYQQVIPQASNQRIDLEREVFLSAIRRTSQLVIDV
                     VSGVKLTFSKNQLEILAQAPEVGEARETMAVKYKGPEISISFNPHFLMDPLKVLTDDE
                     VQLHLTDDTSPGVIRSTVNFIYVLMPIRSS"
     gene            complement(14450..14523)
                     /locus_tag="PKI64_03810"
     tRNA            complement(14450..14523)
                     /locus_tag="PKI64_03810"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14489..14491),aa:Gln,seq:ctg)
     gene            14616..16913
                     /gene="ptsP"
                     /locus_tag="PKI64_03815"
     CDS             14616..16913
                     /gene="ptsP"
                     /locus_tag="PKI64_03815"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE00170.1"
                     /translation="MSDFQKDNVALMCDIGELVGIFDFEQGFDSFLQRAVSMVAWHMR
                     AAVCSLYLYKPATRELVLRANQGLNPQLIGTLRLKIGEGITGMALKEMRPICEARATD
                     NPNFKYIPGSHEENFVSFLAVPIIRRMNPVGVIVVQDPQWNYFTPNDIQALQVIASQL
                     AGVIENADLLSELRQKEQQDSPAPVFHGEQEFGPGTHCVRGVGASDVMGMGLSTRIGQ
                     YLTLDQEAATCPLDLTEDDFDRALLATERQLLDLQRRLEDKIHDAASMIFGAHVLMLK
                     DEAFSGEIRNQIRGGKNPWTAIVEEVRRYSRLFAHSPNPALREKVLDVEDLGHRLLQN
                     LSAQSMASTNPDYSGQIVIAEELLPSDILKISAEGAAGLILLGGGQHSHVAILAKAMQ
                     IPLVIATDRRLLSLPEKTPLLLDAKEGQIIVRPGPEAIQTMQNIIRSLADSLRLAELA
                     QPETRTQDGAEVHLFANMNLVSELDVALRVHAQGIGLYRTEFPFIVRSSFPTEEEQFV
                     VYSRVLERMQGAPVVFRTLDVGGDKVLSYFHAAPESNPFLGLRALRFSLKNPLIFKQQ
                     LRALLRAAHDYPQARIMFPMVASLDEFLEARALVEVCHRELQREGIPSRIPELGMMIE
                     LPSAVTMINELAAAADFLSIGSNDLIQYLLAVDRTNTRMAPWFAPHHPAVIRALHCIV
                     KTARQHNKVISLCGTLGADPRLMPFLLGIGLRHFSLDPLEIPAVQRRIAQINAAQAEQ
                     QAQHMLTLGRIGDVADYLRQINPPD"
     gene            16919..17455
                     /locus_tag="PKI64_03820"
     CDS             16919..17455
                     /locus_tag="PKI64_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00171.1"
                     /translation="MDTLVHGLIGTALCSRTGLPGGRRGPLDAHGPRRWWADWTLWAA
                     FGFGILPDALSLGIYFLSDLLMGRSLTWQGIPPWVFFLYNTTHSLLGMAVILGLVAWR
                     FPALRLPLLAWPLHVLMDVPTHGESVWGTPLLWPFSSWTFSGWNWWLHKEIFIGSWLL
                     AGALWLAVALLRLTGRKS"
     gene            17452..17994
                     /locus_tag="PKI64_03825"
     CDS             17452..17994
                     /locus_tag="PKI64_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE00172.1"
                     /translation="MTFLELVQQRQSVRRYQPGRRIPRDVLERCLEAARLAPSACNSQ
                     PWSFVVVDDPAAVRDLAEAACTHPPFGMNKFARDASALIAVVTEKMKLAARLGAQFRG
                     VQYSLVDLGIACEHLLLQATEEGLGSCWLGWFNERAVKKALEIPRAKKVDLLLCLGYP
                     ADPTPRPKNRHPLDHIRRYA"
     gene            18143..20440
                     /gene="cas3"
                     /locus_tag="PKI64_03830"
     CDS             18143..20440
                     /gene="cas3"
                     /locus_tag="PKI64_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOE00173.1"
                     /translation="MLAERPSVYYARDGQLLIEHLVGVARLAEQFAAPFGLSNAAGSA
                     AKLHDIGKFTKAFQDYLLSSVSGRQVTRGDVIHALQGAKFLASHSRDPMITDILANAI
                     ASHHGGLYDTLSDGIRMLDHKLQRRADELHYDEILDAYQPDISVNEIRNEIVQLCRVF
                     NEHQFPPRFMLHLATKAIFSCLVDADRCDAAGLVPNHTFPDWDVWITQLEKYLAGFMG
                     DGQLNRVRQKISADCQRAGTRAKGIYTLSVPTGSGKTMASLRFALHHARCNRLKRIIY
                     VIPYLSILDQMAQVLEKIFDAACRNQILEHHSNLEISDDSTSPNDEYEQHKLLTARWE
                     NPVILTTMVQFLETIYSNRAASLRKFHNMADAVLVFDEVQALPIKCTYLFNDAINYLH
                     YCGGSSVLLCTATQPQLDRVSRPIKYSPNHALVTLSDAERAVFKRVHIANQSLTPFSF
                     VDIARLAGKEIKAGKSTLVILNTKQSATEVYRLCKTSISNCRTFLLTTNLCPAHRKHQ
                     LAQITNRAKDERILCVSTQLIEAGVDLSFDCVIRAAAGLDSVVQAAGRCNRNGENSTP
                     QTVHVINAVEEKLTRLAEIADGKEVTQRITREAPGISLLQDEALEQFYRYYFFNQQDK
                     MGFPSGPTGQNLYTLLNDNPLASHAYQDQNGGMPYQGLPAAFQSAAEQFAVVDQGQVG
                     VVVQYGNSLHLIEAFEKEYDPLQKMRHLRNLQQFTVNIFPQTQKKLLDAGAICPIQET
                     FYFLSKYWYDSEEYGLLDTPKPAIE"
     gene            20448..21191
                     /gene="cas5c"
                     /locus_tag="PKI64_03835"
     CDS             20448..21191
                     /gene="cas5c"
                     /locus_tag="PKI64_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOE00174.1"
                     /translation="MKKERNTIEYKLYGRYALFSDPIMRVGGEKFSYQVPTYQALVGI
                     TESIYWKPSLFWIIDAVRVMKKIQTESKGVKPIKMSGGNELAYYTYLRDVEYQVRAHF
                     EWSKYRPELQADWDEHKHHNIAKRCVAKGGRRDIFLGTRECQGYVEPCTFGEGEGFYD
                     RYGQLDFGMMFHGFDYPAQTGKKEFGKRLWHAQMKDGVMEFPRPDDSALPRDVIRKMS
                     LKQYTINKDFLPVAHDLALQDLLSEEGGA"
     gene            21188..23071
                     /gene="cas8c"
                     /locus_tag="PKI64_03840"
     CDS             21188..23071
                     /gene="cas8c"
                     /locus_tag="PKI64_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOE00175.1"
                     /translation="MSIWRHLADSYTRNREMLKTTYPLSSTTISNMSDHIIVISVDEH
                     GRFLKAETIPKVSRAEGGVQRIPIPVSEDSMSRTSGAWKNPHPLFEQYGFFKKNDKRF
                     TGSGEVKGYLKQLQEFAHASDHPKIKAIAAYVGRKRLEDDVKGLPSSDKTMVLFKVHV
                     PDDPLTEVWRDPTIFEAWHQYYTQGRKTGLDAVTGEAQNIAIAHPKKISNTVANAKLI
                     SANDTTNFTFRGRFDSANESLSMGYESSQKAHQFLTFLVENRGMVCKEQVILSFSVGS
                     VEQDAPPPLGEEQSIWELIQSAPAAALADAHALLQAETGYDYAQALQAAMQGRAYRNA
                     LEKHPDTIVLALDAATTGRMAITFYRHLSRQDYLEKLLAWHQDCKWIHRFLNRETGRQ
                     VVYAGAPFTSRIVEAVHGKPRGGKDESFIKLKKSANERLLRCIFDGAPLPYDFVRAAI
                     HRASKPMAFSSGGKFNRFAHDELLSTTCALIRKDHKQRTQEEIPLSLDPKRNDRDYLY
                     GRLLGAADKLEEYALHKGQKDRTATAAIRYMQPFAQRPFTTWLTIHNCLRPYIQKVGD
                     SLARQEIQAAHTLFLPGDYENDAPLSGAYLLGYYVQREHIDRTAREISKSKKEKQMEI
                     EHE"
     gene            23064..23924
                     /gene="cas7c"
                     /locus_tag="PKI64_03845"
     CDS             23064..23924
                     /gene="cas7c"
                     /locus_tag="PKI64_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOE00176.1"
                     /translation="MSEALKNKVDFALIIAVNNANPNGDPLNGNRPRQTYDGFGEISD
                     VCLKRKLRNRLQDMGERIFVQSDDRRDDEHRSLNARYAAEKLKDPRVEGCAKWYDVRA
                     FGQLFAFKKKKTKKEEEDGGDADAVSIGIRGPVSIQSAFSVEPVIPETIQIVKSVNLE
                     TNEKEPDKKGSDTMGMKHRVGRAVYVAYGAINTQLAKETGFSAQDAEKLKKAMRSLFV
                     NDESTARPAGSMEVLKIIWWQHNHPNGQYSSAQVHRSLKVAKDGSYELTTLKGLKAEE
                     LSELSGTELD"
BASE COUNT         5168 a         6902 c         7157 g         4822 t
ORIGIN      
        1 atgcgcatcc ggcgcattta ctctgccaaa ggcgcgttta ctttgtccat tgaccttggc
       61 tcgcgcgatt acggcgctct ggtcagattt ttattcttcg gctttggcgg cggctttttc
      121 tttggcggtt ggcttgacgc cggcttcggc atgcttggcg atgaccgcgt cacggatttt
      181 ggcgaagagc gcggggtcgt tacgcagatt ttcgcgggcg gcatcgcggc cttggccgat
      241 ttgctggcca tcgtaagaca gccatgtgcc gcgcttgtcg agcaggccca gatcgttggc
      301 ggcatcaatg accgcgcctt ccttggaaat gccctcggca tatagaatgt caaattccgc
      361 ttcagtgaag gggggggcaa ccttgttttt caccactttg acgcgggtgc gattgccgac
      421 cactttgcca tcgccagctt tgatggcggc aatgcggcgg atatcaaggc ggacggatga
      481 atagaacttg agggcacggc cgccgggggt ggtttcggga ttgccgaaca tcacgccgat
      541 tttttcacga atctggttgg tgaagatgca gcaggttttg gatttgtcga gaatgccagt
      601 catcttgcgc atggcttggc tcatcaggcg agcttgcagg cccacgtggg aatcgccgat
      661 ctggccttcg agttcagcgc ggggggcgag ggcggccaca gagtccacca caatgacatc
      721 cagcgcgttg gagcggatga gctggtcggc aatggtcagg gcctcttcgc cggagtcagg
      781 ttgggagacg agcagattgt caatgtccac gccaattttt tgggcgtagt gcggatcgag
      841 ggcgtgttcg gtgtcaatga acgcgcacat gccgccattt ttctgggcct cggcgatgat
      901 atgcaggacg agggtggttt tgccggaaga ttcggggccg aaaatttcga tgatgcgtcc
      961 acggggaaca ccaccgatcc ccagcgcaat atcaagtgtc agcgcgctgg tggaaatggc
     1021 gggaatgcgg gcaactgcac cctcggtccc caagcgcatg atggcgccct cgccgaactc
     1081 tttttggatt tgggccagca ccgcgctaag ggcgggcggc aggttgctat tggcatcttt
     1141 tttggcggcg gcaggggtct tggggggcat gggttactcc tatatctgaa caattgttca
     1201 ttaacctatc atgggggcca acggctgtca acggcgtatc cttggcacaa atagccgtac
     1261 ggttcaagca cccacaaaat acggcaaatt ggccgcgaac agaaccggcc ggaggaggcg
     1321 gggctcagcc gtagtgggtg gcaatccact tttgatattc gccggagcgg acttgggcgc
     1381 accattcggt gttttgcaaa taccaatcca cggtgcgggc gaatccggtt tcccaggttt
     1441 cggccggctg ccagccgagg tcgcgctcaa ttttggcgca atctatggcg tagcggcggt
     1501 cgtgaccggg gcggtctttg acaaaggtga tcaggctgcg gcgcggctgc ccgttgggga
     1561 gggggggggc cttggcatcc acgagatcgc aaattttttc caccacgcgg agattgggca
     1621 gttcgttgcg gccaccgaca ttataggtgg cgcccggcgc ggcgtgttgc atcacctgcc
     1681 agatggcggc gcagtgatcg gtgacgaaca accaatcgcg cacattcatc ccatcgccat
     1741 aaacagggag gggtttgcct tcgctggcgt taagaatcat gagcgggatc agtttctcgg
     1801 gaaactggta cggcccgtaa ttgttggaac aattggagag ggtggtgggg aggccatagg
     1861 tttcgtgata ggcgcgcacg aggtgatcgc tggcggcctt cgacgcggag tagggtgagt
     1921 tgggcgcgta gggagtttcc tcggtgaaga agccggcagg gccaaggctg ccgaacactt
     1981 cgtcggtgga gacgtggtgg aaaagtttga tttgggccgc gtgctcgcgg gcggcttgca
     2041 gcagggtgta ggtgccgatg atgttggtgc ggacaaaatc atcgggcgcg acgatcgagc
     2101 ggtccacgtg gctctcggcc gcaaagtggg caatggcatc caccgcgtag gtttcaatga
     2161 cctccttcat ggcggcggca tcgcaaatgt ccgccttgcg aaacacgtaa cgctcgccga
     2221 ctgatttttc gagatccagc agcgagagcg gattgccggc ataggtcagc ttgtccacgt
     2281 tgatgatgcg ccgcgggcaa tccggctgcg taaaaagcca acggatgaaa ttgacgccga
     2341 taaatccggc gccgccggtc accagcaagt tctcgaaccg gcggggcatg gtcacgcccg
     2401 gacctcattc aagagggctt cgcggtagcg ctggacatat tcgccaaggt ccgcgcgcca
     2461 gtcctgcatc cggttgagac ccgccttttt gaggttggcg ttttccagaa tggagttggc
     2521 ggggcggacg gtgggggtgg ggtaatcggc ggtggtgcag gggcgcactt cgcaatccac
     2581 cttcatcagg gagatgaatt ccttggcgaa gtcataccac gagcagtggc cttcgcccga
     2641 ggcatggaac agcccggtgg cgtcggcttc gatcacggcc aggatttgct cggcgaggcg
     2701 gcgcgaccac gtcggcgtgc cgaattggtc attgacgatg cgcagcccgc ggcgcgggtc
     2761 ggtgacggcg cgccgcaaaa tggttttgag gaagttatag ccatgcgcgc catagagcca
     2821 ggcggtgcgc aggatggcgt ggcgcggcgc acgctcacga atttcgcgtt cgccggcgag
     2881 tttgcttttg ccatacacgc tgacggggtg gggctgatcg ctctcgagat agggctcggg
     2941 cacggggcgc gcgccatcga acacataatc ggtggaaacg tgcagcagga aaatgtcgta
     3001 agccttggcg cgggtggcca acagggcggg accttcgcgg ttgacgttcc aggttttggc
     3061 cacgtcagtt tcggcgcggt ccacggcggt ataagccgcg caattgacga tgacatcggg
     3121 ccgccagagg ctgaggatgc catccacgct ggcggcttcc gtgatgtcca agttgggcaa
     3181 gtcaagggcc agcacttcgt ggccggtgaa aacttcacgg cagtcccaac cgagttggcc
     3241 catgccgccg gtgacgagaa ttttcatgtt gttgctcctg tgggaagccg ccgccagcgc
     3301 gcgagacatt cggcgaggcg cggatccaaa atggttcggg cttgattgag gatatagagg
     3361 tggatgcccg gcaccccgcc ggccaccagg ccctcgattt gctcggtgag ccacgcgatg
     3421 cccgcggcgc gcgcgtcatc ggcgctaccc gccgccaacg cggtgcgcaa cgccgccggg
     3481 gcggtggcgc ggctaaacgc cagcgagcgc tccaattgcg ccacagacga caccggcatc
     3541 aggcccggca aaatcggcac agtgattcca gcttcacggg cggcggcgac aaattggaaa
     3601 tagaccgcat tatcatagaa aagctgcgtg gtgatgaaat ccgcgccggc ttccactttg
     3661 gttttgagat ggcgcagatc gtccaccagt gaatcggctt cgggatgggt ttccggatag
     3721 cccgccacgc cgcagcagat gtccgggtgg cgctctttga tcagggccac cagatcggcg
     3781 gcataggcca ggccatcggg cgcaggcttg aactcggttt caccacgcgg ggggtcgccg
     3841 cgcaaggcca tgatgttgcg aatgcccgcg gcatggatcc gatccacctg cgcggcgagg
     3901 tccgcgcggg atgcgccaac acacgtcaga tgcgccacca cgggctggaa gcccatccgt
     3961 gccagcagtt cccaggcagc aaaagaaaac tcgcgcgagg aaccgcccgc gccatacgtg
     4021 caggtgacaa aatccggtcg aaccggccgc atccgctcga tggagccgcc aagaataccg
     4081 aaactgagcg gatccttggg cggatagaat tccagcgaaa gcgtcggctg gcgccgcgcc
     4141 gccaaaattt cccggatggt cggccacgtc atagttcagg cagtatagca gacgcgtccg
     4201 gcgggcggca tccatttttt tcggccagca aaaaggtcag gatttcatgg gcagcacgca
     4261 agccggcggt ggccgtcaca caatgcaaac tgcccagtgg ggggcggagc ctgccgcgca
     4321 cataaaaatc ggcggtgggg agcgcggcgg ccgggggtgt cggtttttcg gtggaaaaca
     4381 cgcagcggat accggcgctg ccgccatgtt tggcgaggcg cttgcgcagc aagcgcccca
     4441 aggggcaggt gtgcgaggcc gccaaatcgg ccgaggtgat gcgggcgggc tcgcaacgtc
     4501 gagccgcgcc gagacaagtg aggatgaacg gcacttgggc gcgccgcgcg gccaatagca
     4561 attcggtttt gggatgcagc gagtcaatgg catccagcag cacatcgcac ggctgctcgg
     4621 ccagcacgcg tgcagcggtg tcggcatcca gaaacagagg cagcggcgtg aggtcgagcc
     4681 ccggctgaat atcccgcaaa aaacgcgcag ccacatggac cttgagctgc cccaaggtgg
     4741 actgcgcggc aaagggatgc cgatttagat tggtggcgcg gagttcgtca aaatccacca
     4801 gcaccagatg gcccacgccg gagcgggcga gggattccgc cgccatggcg cccaccgcgc
     4861 ccaagccggc aaccatgaca cgtgcctgcg ccagccgctc cagtgcgtcc gttcccaaca
     4921 gcaactccac acgctccaac gctgaactca tggcgacggc ccatccatgc ggacacccga
     4981 aatcagttgc cgacgtaccc gaactttttc aacagccaat aattgcgcga ccagttgggc
     5041 tccaccttga cccaaagctc cagcttgtgc ggtttgccgt agatttcagc cagcgctttc
     5101 tgggcggcgt ggcgaatatc gcgaatcatt ttggcgcgtg cgccgatgac aatcgggcgt
     5161 tgcgaggatt tctcgacata gatggtggcc tgcacggtca cgccctcatc ggtttccgcc
     5221 agatgatcca cggccacggc gatggcgtgt ggcaattcgg ctttgagccg caagttgatt
     5281 tgtgcccgga tgacatcggc aatgaaaaaa ggccccggat catccgtgag catatcggtg
     5341 ggaaacagcg gttcggcggc gggcattttt tcgcgcaggg cggccagcaa cgcgggcaag
     5401 ccggcacccg tgtgcgcgga aacctcaaac caggtgaccg gcggcggatt ataggccgga
     5461 tcacgggctt gctgggccgt gatggtttcc gcccacgcgg cttcatgctc cgcccgattc
     5521 aagcccaagt ccgttttgtt cagcgccatg aaaagcggcg acatttcggt ggccagtttt
     5581 ttcatccagc cgcggtcttc ctcttgcggc gggcgggaag catccagtag cagcaagatg
     5641 acatctgcgc cggtcaccaa cccacgcgcc gtgcggttga gcagcgaacc cagcgtgtgt
     5701 gtggccttcc ggatgccggg agtgtcgaga aatacgattt ggaggccatc ctcattatga
     5761 atggcgcgga tggggcggcg cgtggtttgc gccacgggcg acaccacgga gacgttttcg
     5821 ccaatgagcg cgttcagcag cgatgatttg ccagtgttgg cgcggccgac aatggccacc
     5881 agcccggccc ggaaagggga ggtattcatg cggttggccg taaatagctg gacaggtcca
     5941 ccacgtcgcc gttggcaggg agttccaccg tggtgtgggg caggcgcccg acgcgctcgg
     6001 ccaaggccgc gcgctgcgat ggttcgccat ggaccagaaa gacgtgcttg ggcttggcgc
     6061 cgcggatata gcgcaacaac tccagccggt cagcatgcgc gctgaaggca ttgagcgttt
     6121 gcacctcggc cttgacatcg aattcatcgc cgaagacacg cacgcgcttt tcgccattct
     6181 gaatacgccg gccgagtgtg ttcaacgcct ggtagcccac cagcaggatc aggttgcgct
     6241 cgttttcgat gccgtgcttg agatggtgga gtatgcggcc atgttcgcac atgccagagg
     6301 cagccagcac aatggccggc tgcgcggagg atgtgatggc tttggattca tccaccgact
     6361 gcgtgaaggt gagccacggc ggactgacga tggagcccag atggttgcgc agcgcttgcg
     6421 attcgttgtc gagataggcc acgtgttttt caaacacgcc ggaaatggcg tgggccatcg
     6481 ggctgtccac atacaccggc acttccggaa tgagaccgcg ctcgcgcagt tgaatcaggt
     6541 gatagatgat ctcttgcgac cggcccaacg cgaaggtcgg aatgagcact ttgccgccgc
     6601 gggccaacgc ccgcacgatg ctctcacgca gggcctcgtg ggcattgagc gcgttgtcgt
     6661 ggagccggtc gccatacgtg ctctcgcaaa tcaggatgtc aatgtcacgg agctgcacgg
     6721 gatcgttcaa cagcggcagg ttgcgacggc ccaaatcaaa cgccagcgca atgcgcagga
     6781 aatcaccgcc gttgtagggg atctgaatca gggtcagcgc cgcgcccaaa atgtggccgg
     6841 catccacgtg ggtgacgaca atatccccgg gcaggcgcag gtggtcgtcg tactcgtggg
     6901 ggcgaatcag tttgagcgcg gcttccgcat cttccatggt gtagagcggc acaatcggcg
     6961 gcaggccccg gcggttggtt ttctggttta ggtaagcggc atccgcttcc tgaatgtggg
     7021 cggagtcgcg cagcatgatc ggcaacaagt ccgccgtggc cgaggtggcg tggatgtcgc
     7081 gtcggtagcc cgctttgacc agcgtgggga tgttgccgca gtggtcaatg tgcgcgtggg
     7141 aaagcaccat ggcctccaca tgttcgggat cgaagcacag ggtctggttg aactccgccg
     7201 attccttgcg ccggccttgg aaaagaccac aatcaaacaa aatccgcgtg ccgttatatt
     7261 ccagcaaatg gcgcgagccg gtcacggtgt cggccccgcc caaaaaatgc cattttaaat
     7321 aggaaccgcc ggaatgggtg ggtgctgagt tcatggatcg aggatagggc aaggcgggtg
     7381 gtggtggcaa cgacaactca tttcacgtcc agtagtggcg atccagcgag cggtattgaa
     7441 tggcctcggc aatgtggtcc gccgcgattt gctcctgtgc ggccaaatcc gcgatggtgc
     7501 gcgccacttt gagaatccgg tcatacgccc gcgcgctcag gtgcagatcc gccatcgccg
     7561 ctttgagcag tccctgcgcg gtttcgtcca ggacgcagtg cttgtggaca tcgcgcgcgc
     7621 gcatcccggc gttgcagtga atgacgtggg ccccgcggct cctggcgaaa cgctcacgct
     7681 gaatcgcgcg cgacgccacc acacgctcgc gaatcgccgc cgagctttcg cctttctcca
     7741 gcgaagacaa ctgctggtat tccatggccg gcacttccac gtgaatgtca atgcggtcca
     7801 gcagcgggcc ggaaattcgg ttgcggtagc gctgcatttg gatgggggtg cagcggcagt
     7861 ttttggtggc atcgccaaaa tggccgcacg gacagggatt catggcggcc accaacatga
     7921 aacggcaggg gaaggtgacc gaagccgccg cgcgggcgat ggataccgtg ccatcttcca
     7981 gcggctggcg cattacctcc agcacgttgc gatggaattc cggcaactcg tcgaggaaca
     8041 gcacgccgtt atgcgccagg ctcacctcgc ccggcaacgg atgcgcgccg ccccccagca
     8101 agccggcatc ggaaatcgtg tggtgcggcg aacggaatgg cctaccgctc accactgcct
     8161 gatgcgcagg caacacgccg gcaatgctgt ggattttggt cgtctccagc gcttccgaca
     8221 acgtgagtgg cgggaggatg gtcggcaccc gcttggccaa catggatttg ccggtacccg
     8281 gcggcccgat cagcagaata ttgtgcccgc cggccaccgc cacctcgatg gcgcgccggg
     8341 cgaattcctg gcccttcaca tcggcatagt cgtcgtcgta ggccgcgccg ctgccgaagg
     8401 ccgtctgcat atccacgcgg aacggttcga ttgttttttt gccatccagg aaatccgccg
     8461 ccgcgcgcaa tgtggccacg ggatagacgg ccagcccttc caccacagcg gcctcttccg
     8521 cgttttccgg cggcaccaga atgccttggc gaccggcggc gcgggcttgc agggcaatgg
     8581 gcaacacgcc acgtacgcgg cgcacctcgc cgctcagcgc cagctcgccc gtcacgcaaa
     8641 actgggcaag gcgtgcccca tccatctgtt cttccgccgc cagcatgccg agggcaatcg
     8701 gcagatcaaa gctgggcccc tccttcttga tgtcggccgg cgccagattg accgtggttc
     8761 gccccatcgg tggtgaaaat ccggaattga ttagtgccgt ccaaacgcgg tcggaactct
     8821 ctttgaccgc cgcatccggc aaccccacca caaccacctt gggctctccg tgcccggcat
     8881 tgacctcgat ctcaacctcg tacgcatcca ccccgtagac tgcgcctgaa aacactttcg
     8941 ccagcataag gggttagtcc cgtgttttga tgtttttgac tttaacgaac ggctgcccgc
     9001 cccactcgcc gcgctgggcc atcgtcacct ttttcatgaa ctccgcaaag tctatcgcct
     9061 tgtccatata ccgctgctcc ttgatccagc cgttgtggtc caccgtattt ggtctcaccc
     9121 cccaagccaa ggcatatttt tcagggcagg tcttgtacat ggtcacccca gcagactccg
     9181 tcacgcgcca cacggccgcc cagccggcga tgattcgcca tgtttctgtg gtcagggctt
     9241 gaattgggcc agaaagttgt cgaggtcttc gccgccgggc tggccggggg taagccagga
     9301 ggagtcgctg tgggcgccgc caagaatggt gaatgtcttg ggcgcggcgg cagcggcaaa
     9361 gagaaggcgg ccgctttgga aggggaccac ttcatcgcgc gggctatggc caaacagctt
     9421 gggaatcccg ggtagcgcgg cggcagtggc ggcggcatca tatttcacgg tcaagaggcg
     9481 gtcgagaggc caatcgggaa agcggcggcg ggccatgtcg gccatggagg caaacccgcc
     9541 ttcaatgatg aggccgctga cgggtcgctc gcgggcgagc tggcaggcca ccgccgcgcc
     9601 caacgaatgg ccatagacga tgatggggag gttgcggctc atgggctggg cggcatcgaa
     9661 cgcggcgcgg gcatcgcggt agaggccggg ttcggaaggg ctcccagcgc tttggccata
     9721 gccgcggtaa tcccacaaaa acaggttgaa gccgcgtttg gtaaaggcgg gggccaaatg
     9781 ggcgcgatcg ctgatgttgc cgccgttgcc gtggcaaaag actactgtgc cgcgcgggtg
     9841 gttggccgga atccaccagc cggagagcgt ggtgccatca gcggcggtga actggacttc
     9901 ctgaaagcgc tgttgaaagt gggcgggggt ggcgtggatg tcggggcggg gatggtacaa
     9961 ctgctggcgc tcaaagcgta gcaggaggaa gccgccgccg accgcagcca gcaggatcac
    10021 caaaagcaac gtcgtcaaaa attttttcat gcggcctata cttccagttt tttcggaaag
    10081 gtagccagca gtttatagcc gcgttgggtg atgaccacag tatcttcgat gcgcacgccg
    10141 ccgataccgg gataatagag cccgggctcg acggtgacga cgtggccggc gagcaattgg
    10201 cccggccccc ggctcaagcg cggctcttca tgaatatcca gtcccacccc gtggccggtg
    10261 ctgtggatga agccgcattc gcgccccggt ggttccagct tggtgatgaa tcccgcgcgc
    10321 aaaaaagaat ccaccacggt ttgatggact gccgccaatt cgacgccggg gcgcagcatg
    10381 gcgagcgcgt cgcgttgcgc cgtgtgaacg gcaagataca tctgacgcac ttggggagtg
    10441 gcttcgcccc gtaccaccgt gcgggtgatg tcgccccaat atccggtttc ccggtggcgc
    10501 ggaaagatat cgaggacaat aggcacgccg gcgcgtagtg gcccgctgcc agtgtcgtgc
    10561 ggacgcgcgc tttggggtcc aacggcgaca atggttcctt gatcagcgca ctggtgttcc
    10621 atcaaggcgt gggcgatgac gttcttgatg tgctccgcgg tcagaatccg gccgcggtgg
    10681 cgcaacactt tgcgcgggcc aatggttgca gcgcgaatgc actcgatggc cgcgcgcatg
    10741 gcggccacag cggcttcctg ggcctgcgtg atccacgcca cctcacgcgc ggttttgatg
    10801 gcacgcgacg gaaatggcgg tgcgtcctga acctccacag ccacgccggc ggcagtcaat
    10861 gcgcgcgcca caccaatggg aaaatggggc gtgacggtca cgcggcgcac gcccaacttc
    10921 gcggccaaag caacaaactg ctgggacaat gtcgcgggcg gaggaatccc cggcgaggcc
    10981 aacgaagcgg gggtatggat gattgcccgc gggcatgttt tcttggcgcg ggaggcttcc
    11041 aaaggcgaca cgagcagatg taaacggccc ccgggttcca ccaacaggcc gaaggggtcg
    11101 ctggcagcca tccccgtggc atggcggaca tcgttttcat ccaagcctgt ggccacaaaa
    11161 atgagggggg ctgttgattt gttcatagtt tggctggttt tcatgaaaaa aggattgtca
    11221 atagcttgat tttcaatgga tttccggcgg ttttcaatcc aaaaatccgc ccggatcaaa
    11281 gtacgaggca attatttgat aagtaccgga tcaactaaat attgtgttat taaaactaca
    11341 agccactaca atttgttgtg tgatgaaata tcttggaaaa tggggggagg gggcttgtcg
    11401 gattgcaaag tggttattat ggtcacgact gtcttcgggc agcagatcga aaaacggctg
    11461 tgaataagct gtggataagt gcgccggagg gccattttag cgggctccgc gtggggtccg
    11521 gggaatgcga aaaacagggt gttttaggat tgagtcggcc gggccattcg tgcgagactg
    11581 gtggtccggg ctggaataag gatttttgtt gtggacgaaa agaaggccaa agaggtttgg
    11641 gcaaaggcgt gcgagcatct tgccgaggcg gtctcggcgg atattttcag ccggtggatc
    11701 ggggtgattg aagccgcagg ggatgatgca cccggccaac tgacgctgac ggttcccaac
    11761 gggttctacc aagactggct ggaagaacat tacctgccga tgatccgcaa ggcgctgggt
    11821 gctgcaggcc acgagcacat agccattgcg tttgtggtaa atgataacgg cccggcgccc
    11881 gccccggcca cgctggtcaa gagcaatgaa ccagctccag ccaaacgccg tgggccaaaa
    11941 atctacaacg tgggcgggac gctgaacccg cggcacacgt ttgaaaactt tgtggtgggc
    12001 ccggcgaaca cgtttgccca cgcggccgcg ttggccgtgg tggatgcacc cgcgcgcgct
    12061 tataatccac tgttcattca cggggggacg ggtctcggca aaacgcacct gatccaggcc
    12121 atgggccacg cgctcatcaa gcgcggcaaa gggaccgtct gctacaccac ctgcgaagat
    12181 ttcaccaacg agttcatcca agccgtacag caacacgagc tcaccagctt ccgccggaaa
    12241 taccgcaaca ttgatttgct gctgattgac gatatccatt tcctggcccg caaagagggc
    12301 atccaggaag aatttttcca tacgtttaat tcgctgcatc aaaaccaaaa acaaattgtg
    12361 atgaccagcg accgcccggt gagcgaaatt gccaacctgc agaaccggct cgtctcccgc
    12421 tttgaatggg ggatggtgac ggaaatggaa atggtggatt tggaaacccg ggtggccatc
    12481 ctgcggcaga agcgggagga gttgggcatc cagttgccgg atcccaccct gttcttcatt
    12541 gcggaacacg tgcgctccaa catccgcaag ttggaagggt cgctgctgcg ggtggcttcc
    12601 tacgcctccc tgacgcgcaa gccgctgacc accgaagcgc tggagtattt gctgcgcgac
    12661 accatcgagc aggagaacca aaccgccgtc acgctcgaag gaatccagcg gattgtcgcg
    12721 gaatattacg acatccggct gggcgacatg accagcaagc agcggcccaa caacatcgcg
    12781 ttcccacggc aggtggccat gtatttgtgc cgggaaatga cggagcaatc cctgccggcc
    12841 atcggcaacg catttgggcg caaccacgcc acggtgctgc acgcgcaccg gagcatcggc
    12901 gccaagatga aaacggactc ttcgcttcgc cagacgattt tgtcgttgca gcagcggctg
    12961 gggggcgccg cggcaccggt ttgacatgac ccggcgccac gcccattcgg gagcagacgc
    13021 acgggggacg acgcgacctc atggattgtg gacaaggttg tggatagcgc gggataagtt
    13081 gtggaaaaga acccaaagct gtgaatgggg ccggagtcat ccacaccatg cactttccga
    13141 acacaggttt tttgacaggc tgaattcgac ggaagtcctt ggaagaccca ttctttgctt
    13201 gctgtccaca caattcacag gacatactga taagacggtt tataaattta tatagatact
    13261 agtaaccgac accgggaggc gtcatgaaaa tcaaagtcaa aaaagaagcg atgttggcat
    13321 cgttgcaaaa agttcagtcg gtcatcaatc cgcgcaatac cctggccgtg ctggccaacg
    13381 tgttgttccg cgcccaggac ggcaagttgg aactgaccgc caccgatatg gcgctgacgt
    13441 tgcgcaccac catggaagcc gaagtcctcc agcccggctc caccaccatt cccgccaagc
    13501 ggattctcgg ggttttccgg gaattggccg tggccgaagt cgagctggaa gtcaacgacc
    13561 agaacatcac cctcatccac gccggtagct ctcacttcaa aatccatggg atttccgaaa
    13621 acgaatatcc caatctgccc gaactggcgg atgccaagac ctacaccttg gaccaaggcg
    13681 acttcaaaaa catgttgaag ctgacctcct ttgcagcctc cctggacacc aaccgccaat
    13741 tactgaacgg ggtgctgttg agcttcaaga acgggaagtt gagcgtggtc gccacggata
    13801 gccggcgcct ggcccatgtg gagcaggaag tcgagttccc ggcggatgcc gccttggact
    13861 tgattgtgcc cctcaagacc gtggatgaac tcctcaagac cctcggcgat tccggcccgc
    13921 tcaaaatcat ggccaccccg aagcaggtgg gctttgactt caactccgtc ttcatcgttt
    13981 ccaagctgga agaaggcaaa taccccaact accagcaggt gattccccaa gcgagcaacc
    14041 agcggattga tttggagcgc gaagtgtttc tcagcgccat tcgccgcacc tcacaactgg
    14101 tgattgacgt ggtctccggc gtaaagctga ccttcagcaa gaatcaattg gaaatccttg
    14161 cccaggcccc cgaagttggt gaagcccgcg aaaccatggc cgtgaagtac aaaggcccgg
    14221 aaatttccat ttccttcaac ccccacttcc tgatggaccc cctcaaggtg ctcaccgacg
    14281 acgaagtcca attgcacctg acggacgaca ccagccccgg ggtcattcgc tccaccgtca
    14341 acttcattta tgtgttgatg cccatccggt cgagctaact cccccttggg cttcgtgctg
    14401 atccccaaaa accagcacag ccgtcccatc cggggcggct gtgatcgttt ggttgcccga
    14461 ctaggatttg aacctagaca aacagatcca gagtctgctg tgctaccgtt acaccatcgg
    14521 gcaagttgaa aatgaaacgt ggagaaggta ctttttccag ttatgaagtc aagcacgggc
    14581 atcccaaccc ggaatccccc ttggactaat ttgccgtgag cgatttccaa aaagacaacg
    14641 tggcgctgat gtgcgacatc ggcgagctcg tcggcatctt tgacttcgag cagggatttg
    14701 attcgttcct ccagcgtgcg gtttcgatgg tggcttggca catgcgcgcc gcggtttgct
    14761 ccctctatct ctataagccg gccacccgcg agctggtcct gcgcgcgaat caggggctga
    14821 atccccagct cattggcacc ctgcgcctca agattggcga gggcatcacc ggcatggcgt
    14881 tgaaggaaat gcggcccatc tgcgaggccc gcgccaccga caaccccaac ttcaaatata
    14941 tccccggcag ccacgaagaa aactttgtct cgttcctagc cgtgccgatc attcgccgga
    15001 tgaacccggt gggggtgatc gtagttcagg acccccagtg gaattatttc acccccaacg
    15061 atattcaggc cttgcaggtc atcgcctccc agttggccgg cgtgattgaa aacgccgact
    15121 tgctcagcga gttgcgccaa aaagagcagc aagactcccc ggcccccgtg tttcatgggg
    15181 agcaggagtt tggccccggc acccactgcg tccgtggggt gggcgcctcg gatgtcatgg
    15241 gcatggggct ttcgacccgt attggccaat acctgacgct cgaccaggaa gccgccacct
    15301 gtcccctcga tctcaccgag gacgatttcg accgtgccct gctcgccacc gagcgccaac
    15361 tcctcgatct ccaacgccgc ctcgaagata aaatccatga cgcggcctcc atgatttttg
    15421 gcgctcacgt cctgatgctc aaggatgagg cgttttccgg cgaaatccgc aaccaaatcc
    15481 gcggcggaaa aaatccgtgg acggccatcg tggaggaagt ccgccgctac tcgcgcctgt
    15541 ttgcgcacag ccctaatccg gccctgcgcg aaaaagtgct cgatgttgaa gacctcggcc
    15601 accggctcct ccaaaacctc tcagcccaaa gcatggccag caccaacccg gattactccg
    15661 gccaaatcgt cattgccgaa gaattgctcc cctccgatat cctcaaaatc tccgccgaag
    15721 gcgcggcggg cctgattttg ctgggcggcg gccagcactc ccacgtggcc attctggcca
    15781 aggccatgca gattccactg gttatcgcca ctgaccgccg cctgctctcg ttgcccgaaa
    15841 aaacgcccct gctgctggat gccaaggaag gtcaaatcat cgttcgcccc ggtccggaag
    15901 ccatccagac catgcagaac attatccgct cgctggccga ctccctgcgc ctggccgagc
    15961 tggcccagcc ggaaacccgg acccaggatg gcgcggaagt tcacctgttc gccaacatga
    16021 acctcgtcag cgagctggat gtcgcattgc gggtccacgc gcagggcatc gggctctacc
    16081 gcaccgagtt tccgttcatc gtccgcagca gcttcccaac cgaggaagaa caatttgttg
    16141 tctattcccg cgtgctggag cggatgcagg gcgcgcccgt cgtcttccgc accctggatg
    16201 tcggcggcga caaagtcctt tcctacttcc acgccgcgcc cgaatccaac ccgttccttg
    16261 gcctgcgcgc cttgcggttt tcgctgaaaa atccgctgat tttcaaacag cagttgcgcg
    16321 cgctgctccg cgccgcgcac gattaccccc aagcccgcat catgttcccc atggtggcct
    16381 cgctggatga attcctcgaa gcccgcgcgc tggtcgaagt gtgccaccgc gaactccagc
    16441 gcgaaggcat ccccagccgt attcccgaac tcggcatgat gattgaactg ccctccgccg
    16501 tcaccatgat caacgaactc gccgccgccg ccgatttctt gagcatcggc agcaatgacc
    16561 tgattcaata tctgctggcc gtggaccgca ccaatacgcg gatggcgccg tggtttgccc
    16621 cgcaccaccc cgcggtgatt cgcgcgctgc actgcatcgt gaaaaccgcg cggcagcaca
    16681 acaaggtcat ttcgctttgc ggcaccctcg gcgcggaccc gcggctgatg cctttcctgc
    16741 tcgggatcgg ccttcggcac ttcagccttg atccgctgga aattccggcc gtgcaacggc
    16801 gcatcgccca aatcaacgcg gcccaagccg aacagcaggc ccaacacatg ctgaccctgg
    16861 gccgaatcgg cgatgtcgcc gactaccttc gccaaatcaa cccgccggac tgaacccggt
    16921 ggatacgctg gttcatggcc tgatcggcac ggccctgtgc agccgcaccg gattgcccgg
    16981 cggccggcgc ggcccgctgg atgctcacgg gccccgccgc tggtgggccg attggaccct
    17041 ctgggccgcc tttgggttcg gtattttgcc cgacgccctc tcgctgggga tttacttcct
    17101 gagcgacttg ctcatgggcc gcagcctcac ctggcagggc attccgccgt gggtgttttt
    17161 cctctataac accacccaca gcctgcttgg gatggccgtg attctcggcc tggtcgcgtg
    17221 gcggtttccc gccctgcgcc tgcccctgct ggcctggccg ctccacgtct tgatggatgt
    17281 gcccacccat ggcgaaagcg tttggggcac gccccttctc tggccttttt cctcgtggac
    17341 gttcagcggt tggaattggt ggctccacaa ggaaatcttc attggcagtt ggctgctggc
    17401 cggcgccctt tggcttgccg tggccctcct gcgccttacc gggagaaaat catgaccttt
    17461 ctggaactgg tgcaacagcg ccaaagcgtg cggcgctatc aacccggacg ccgcatcccg
    17521 cgtgacgtgc tggagcgttg cctcgaggcc gcgcgcctgg ccccctccgc ctgcaattcg
    17581 caaccgtggt ccttcgtggt ggtggatgac cccgccgccg tgcgcgacct cgccgaagcc
    17641 gcctgcaccc acccgccctt cggcatgaac aagtttgccc gcgacgcctc ggcattgatt
    17701 gccgtggtca ccgaaaaaat gaaacttgcc gcgcggctcg gcgcgcaatt ccgcggcgtg
    17761 caatacagcc tggtggacct gggaattgcc tgcgaacacc tgttgctgca agccacggaa
    17821 gagggccttg gcagttgctg gctggggtgg ttcaacgaac gcgccgttaa aaaggcgctg
    17881 gaaattccgc gcgcgaaaaa agtggatctg ctgctctgtc tcggctaccc cgccgacccc
    17941 actccgcgcc ccaaaaaccg ccaccccctc gaccacatcc gccgctacgc ctaatcccgt
    18001 ccacgggcgg ctccgacgct atggagccac gggattgatc cttgagtttg ccccggttcc
    18061 cggcctatta tcgagccgct atgaagtacg cgcatagcct caaagaacgc cccccggagc
    18121 agtggcagcc acggagggtg aaatgctagc tgaaaggcca tcggtgtatt atgcgcgtga
    18181 tggccaatta ctaattgaac acctggttgg tgttgctcga cttgccgaac aatttgcagc
    18241 cccctttggt ttgagcaacg ccgctggatc ggctgccaag ttgcatgata tcggcaagtt
    18301 tacaaaagcc tttcaagatt atctattgag cagtgttagc gggcggcaag tcacccgtgg
    18361 ggatgttatt catgcgctgc agggggccaa atttctggcg agtcacagtc gtgacccaat
    18421 gattaccgat atcttggcca atgctatcgc ttcacatcat ggtggtttat acgatacact
    18481 ttcagatgga atccgaatgc ttgatcataa actccaacgg cgtgcggacg aactccacta
    18541 tgacgagatt cttgatgcgt accaaccgga catttctgtg aatgaaattc gcaatgaaat
    18601 tgtccagtta tgtcgagttt tcaacgaaca tcagtttccg ccccggttca tgctgcacct
    18661 tgcaaccaag gcgatatttt cgtgtctggt agacgccgat cggtgcgacg ccgcgggact
    18721 cgtgccgaat catactttcc ccgattggga tgtctggata acgcaactgg aaaaatatct
    18781 tgccgggttt atgggggatg gccagttgaa cagggtgcgt cagaagattt cagcggattg
    18841 ccaacgggcc ggaacaagag ccaaaggcat ctatactttg tccgtaccaa caggctccgg
    18901 caaaacgatg gccagcttga ggtttgccct ccatcatgct cgatgcaatc gcctcaaacg
    18961 catcatttac gttattcctt acctatccat tttagatcag atggcacaag tgcttgagaa
    19021 aattttcgat gcagcatgtc gcaaccagat tcttgaacac cattccaatc tggaaatctc
    19081 agacgattct accagcccta atgatgagta tgagcaacat aaacttctca ccgcccggtg
    19141 ggagaatcca gtcattctga cgacgatggt ccagtttctt gagacgattt atagcaaccg
    19201 cgcggcaagt ttacggaaat tccacaacat ggctgatgct gttttggtat ttgatgaggt
    19261 gcaagcgtta cccatcaagt gcacctatct gtttaatgat gccatcaatt atttgcacta
    19321 ttgtggggga tccagcgttc tgctttgcac ggcgactcag cctcaattgg accgtgtgtc
    19381 ccgcccgata aaatattcac ccaaccacgc gcttgtgacc ctgtctgacg cggaacgggc
    19441 agtattcaaa cgtgttcaca tcgccaatca atctttgacg cctttttcat ttgtggatat
    19501 tgcccggttg gccggaaaag aaataaaagc cggaaaaagc accctggtca ttctcaacac
    19561 caaacaatcc gccaccgaag tataccggct ctgtaaaacg tcaatatcta actgtcgaac
    19621 tttcctgtta accaccaacc tatgcccagc gcatcgtaag catcagcttg ctcaaataac
    19681 aaatcgcgcc aaagatgagc gcattttatg tgtcagcact caattgatcg aagcaggagt
    19741 tgatttgtca tttgattgcg taattcgggc cgccgctgga ttggatagcg tagtgcaggc
    19801 cgcaggacgg tgcaaccgta atggagaaaa ttcgacgccc caaactgttc acgtcatcaa
    19861 tgccgtggaa gagaaactca cccggcttgc tgaaattgcc gatggcaagg aagtgaccca
    19921 acgaatcacc cgcgaagctc cggggatctc acttcttcaa gatgaagcac tcgaacagtt
    19981 ctaccgttac tactttttca accagcagga taaaatgggg ttccccagcg gcccaacagg
    20041 ccaaaatctt tacaccctcc tcaatgacaa tccgcttgcc agccatgcgt atcaagatca
    20101 gaacggcgga atgccatatc aaggcctacc ggccgcattc cagtcagccg cagagcagtt
    20161 tgcggtggtt gatcagggcc aggtgggtgt tgttgtccag tatgggaact ctctacatct
    20221 gattgaggcg tttgaaaaag aatatgaccc tttgcaaaag atgcggcacc tccgcaatct
    20281 gcagcagttt acggtcaata tctttcccca gactcaaaaa aagcttcttg atgccggggc
    20341 gatatgcccc atccaagaaa ccttttattt cctatccaag tattggtacg acagcgaaga
    20401 atacggcctg ctggacacac ctaaaccagc aatcgaatga ggcgtacatg aaaaaagaac
    20461 gaaacaccat cgaatacaaa ttgtacggac gctatgccct cttttcggat cccatcatgc
    20521 gcgtgggggg cgaaaagttt tcttatcagg ttcccaccta ccaagccttg gtgggaatta
    20581 cggaaagcat ttactggaaa ccgagtcttt tctggatcat cgatgcggta agagtaatga
    20641 agaaaatcca aaccgaatcg aagggggtta agccgattaa aatgtcaggg gggaacgaac
    20701 tcgcctatta cacctatctg cgggatgtgg aataccaggt gcgggcccat tttgaatgga
    20761 gcaaatacag gccagagctg caagccgact gggatgagca caagcaccac aacattgcca
    20821 agcgttgtgt ggccaagggg gggcgccggg acatctttct gggtacgaga gaatgccaag
    20881 gctatgtgga gccatgtaca ttcggcgagg gagagggttt ttacgaccgc tatgggcaac
    20941 tcgatttcgg catgatgttc cacggatttg attatcccgc ccagaccggg aaaaaagaat
    21001 ttggcaaacg gctgtggcat gcccaaatga aggacggcgt gatggagttt ccccgcccgg
    21061 acgactccgc cctgccgcgc gatgtcattc ggaagatgag cttgaaacaa tacaccatca
    21121 acaaggattt cctgccggtc gctcatgatc tcgcgctgca ggatttattg agcgaggagg
    21181 gcggggcatg agcatatggc ggcatttggc ggacagctac acccggaacc gggagatgct
    21241 taaaacaaca tatccgttgt cgagtacgac catcagcaat atgtcggacc acattattgt
    21301 gatatcggtt gatgaacacg gcagattcct gaaagcggaa accataccga aggtaagcag
    21361 agccgagggt ggggtccagc gaattcccat tccggtgtcc gaagatagca tgtcccgcac
    21421 ttcgggcgct tggaaaaacc cccacccgtt gttcgaacaa tacggttttt tcaaaaaaaa
    21481 cgacaagcgt tttaccggaa gcggggaagt gaaaggatac ttgaagcaac tccaggaatt
    21541 cgcccatgcg tccgaccatc cgaaaatcaa agccattgcg gcttatgtag gccggaaacg
    21601 gttggaagat gatgtcaaag gtcttccttc aagtgataag accatggtgc tgtttaaggt
    21661 gcacgttccg gacgatccgc tcacggaggt ttggcgcgat ccgacaattt tcgaggcttg
    21721 gcatcaatat tacacccaag gaaggaagac cggtttggat gcggttacgg gtgaggcgca
    21781 aaacatcgct atcgcacacc ccaaaaaaat atccaatacg gtggccaatg cgaaattgat
    21841 ttccgccaat gacacgacga actttacctt ccgggggaga tttgacagcg caaacgaatc
    21901 tttgagcatg ggctatgaat cctctcaaaa agcgcaccaa tttcttacgt ttctggtcga
    21961 aaatcgcggc atggtttgca aggaacaggt cattctttcc ttttccgtcg ggtcggtgga
    22021 acaagatgcc cccccgccgc tgggcgaaga acagagcatc tgggaactca tccagagcgc
    22081 gccggcagcg gcactggctg acgcgcacgc ccttttgcag gcggaaaccg gctatgacta
    22141 cgcacaggcg ctgcaggccg ccatgcaggg cagggcatac cggaacgcgc tggaaaagca
    22201 tcccgacacg attgtactgg cattggacgc cgccacaacg ggaagaatgg ccattacctt
    22261 ttacaggcac ctgtcccgcc aggactacct cgagaaattg ctggcgtggc accaagattg
    22321 caaatggatc caccgcttcc tgaatcgcga gacaggtcgg caggttgttt atgcaggcgc
    22381 accgtttaca agcaggattg tcgaggcggt tcacggcaag ccacgcggag ggaaggatga
    22441 aagcttcatc aagttgaaaa agtctgcgaa cgagcggctt ctgcgctgca tattcgacgg
    22501 agcgcctctg ccttatgatt ttgtgcgggc cgccatccat cgtgcctcca agccgatggc
    22561 tttctccagc gggggtaaat tcaatcgctt cgcgcacgac gaacttcttt caaccacttg
    22621 cgctttgatt agaaaagacc acaaacaacg aacacaggag gaaataccct tgagcctaga
    22681 cccgaagaga aatgatcgcg actacctcta tgggcgactg ctcggtgccg cggataaact
    22741 tgaggaatat gcacttcata agggccaaaa agaccggact gcaacagcgg ctatccgtta
    22801 tatgcagcct tttgcccaac gaccatttac tacatggtta acaattcaca attgcctgcg
    22861 cccctacatc cagaaggtgg gcgactcttt ggcccgccag gaaatacaag cggcccacac
    22921 gcttttcttg ccaggtgatt atgaaaatga tgcgccgttg tccggagctt acctgctcgg
    22981 ttactatgtt cagagggagc atattgacag aacagcgcgc gaaatcagca aatctaaaaa
    23041 agaaaaacaa atggagatag aacatgagtg aagccttaaa aaacaaagtg gactttgcgc
    23101 taatcattgc tgtaaacaat gccaatccca acggggatcc gctcaatggc aaccggccac
    23161 gccagacgta tgatggcttc ggcgaaatat ccgatgtctg tttgaagcgt aaactccgca
    23221 atcgactgca agatatgggc gaaaggattt ttgtccagtc ggatgaccgc cgggatgacg
    23281 aacaccgttc gctcaacgcc cgctatgcag cggaaaaact gaaagatccg cgggtggagg
    23341 ggtgcgccaa gtggtatgat gtccgcgctt tcggccagct gtttgccttc aagaaaaaga
    23401 agaccaaaaa agaggaagag gacggcggtg atgccgatgc cgtttcgatt ggcatccgcg
    23461 gcccggtcag catccaaagt gcatttagtg ttgaaccggt tattccggaa accattcaga
    23521 ttgtgaagag cgttaatctg gaaaccaacg aaaaagaacc ggacaaaaaa ggttcggaca
    23581 ccatgggaat gaagcatcgc gtcggccggg cggtatatgt cgcatacggc gccatcaaca
    23641 cccagctggc gaaggaaacc ggtttttctg cacaagatgc cgagaagctg aagaaagcga
    23701 tgcgatcatt gtttgtgaat gatgaatcaa ctgctcgccc tgccggcagc atggaggttc
    23761 tcaagattat ttggtggcag cacaatcacc cgaatgggca atactcctct gctcaagttc
    23821 atcgttccct gaaggtggcc aaagacggaa gctatgaact gactacattg aaagggttga
    23881 aggcggagga gctttctgaa ctttccggaa cggaactgga ttaaacaatt catatccttc
    23941 gcaacgctgg gcggagatga gtatatttct gtcgggcgtt gtgggtggct ttggaggaaa
    24001 ggagccaaac gatgtcgtct tcgcccattg ggctttcgca tggttaccg
//
LOCUS       DAOPNP010000027        23718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2682_length_23718_cov_8.918269, whole genome shotgun sequence.
ACCESSION   DAOPNP010000027 DAOPNP010000000
VERSION     DAOPNP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23718
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_23718_cov_8.918269"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKI64_03850"
     CDS             <1..488
                     /locus_tag="PKI64_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00177.1"
                     /translation="PDRGRLLAAVLTVLHELGIAAEEGPRGGVYVAGRKIGGTAAAAG
                     KGVLLFHGTLLVTTDLTALTAALAAHSPEYGGETGHAARAGVASVPSPVTTLAHLRPG
                     LDVATVEAALFAGLARAGAPQPPETFLDLSNIRFLATDYGRESWILNRTPPARQTRGV
                     P"
     gene            485..928
                     /locus_tag="PKI64_03855"
     CDS             485..928
                     /locus_tag="PKI64_03855"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE00178.1"
                     /translation="MKEKDPLRALRALLDTIRVGVLCTVSKDAQPRCRWMTVAALPRV
                     QGCFYCVTSKGSAKVKEIEANARVQWTFQTPTLDEIISVCGQAEVLQNPGLKAEVLEA
                     LGPNLTIFWRVNPDPAKLVVIETVVDNAKLFRPMECLYVEKEAGT"
     gene            925..1938
                     /gene="pdhA"
                     /locus_tag="PKI64_03860"
     CDS             925..1938
                     /gene="pdhA"
                     /locus_tag="PKI64_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOE00179.1"
                     /translation="MKELNGELLWELRNNMVLMRVFEEKAGQMYGLRKIGGFCHLYNG
                     QEAIAAGFGSVLDYSRDYVLTGYRDHGHALAAGMEPRAIMAELFGKVTGCSRGKGGSM
                     HLFDARRHFLGGNGIVGAQIPVAAGVAFAQKYLKTGGATVVFFGDGAIHQGAFHEAMN
                     LAKIWNLPLVSVCENNQWGMGTSWKRVSSVDDFSVMATAYGMRGELVDGMDVLEVRDA
                     AGRALAEAREGHPVLIEARTYRYKGHSMSDPQKYRTREEVEKYKREQDPILILETLMK
                     EAGHWDDARAAAQDRAAEEISKESVDFAERSAEPALHTLYEDIYAGEPFFMAPSNPAT
                     LVT"
     assembly_gap    1961..2060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2061..2819
                     /locus_tag="PKI64_03865"
     CDS             <2061..2819
                     /locus_tag="PKI64_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOE00180.1"
                     /translation="VEWMTHNFALLALDQVVNEAAKMRHMSGGQFHVPVVFRGPNGPA
                     EYLSSQHSQALASYWVHVPGIRVVAPAFPADAYGLLKSAIRDPDPVVVLEAEMLYGFK
                     AEVPDEEFLIPIGKANTLRNGNDITLVSFGKPVHLLLQAADELARDNIAAEVIDLRSL
                     RPLDEETLMASVARTGRAVVVDESWPAASVGSYVAHRIATACFDLLDAPVEFVASEDV
                     PMPYNHTLELAVQPSVKKIVAAARRALYLEVPHG"
     gene            2812..4125
                     /locus_tag="PKI64_03870"
     CDS             2812..4125
                     /locus_tag="PKI64_03870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOE00181.1"
                     /translation="MADPVLMIALSPTMNEGTIVQWFAQEGQEIKTGDALCEVETDKA
                     TMTYEAPSAGTLLKIIAGPGTSAVVGQLIAVVGKPGDNWEEIAAKAQAAAPAAPAQPA
                     APAAAPPAPPPPPAPAPAPAPAVAAPVVASVSGAAPQLAPGVPRSSPVARKLAQEKGL
                     DLRGIRGSGPGGRIIARDVEQAHAAPAPAMAAVAPRPAGSAPAAATPQSRGLRGDRVP
                     LSKMRSIIATRLHQSYSQAPHYFVRVAIEMDRLLDLRKQLNAGRDPALSLNAFIVKLA
                     AAALERNPGINASWEGDAIQYLPHADIGLAVALPGGLITPVIRACEAKGLEAIDQDVG
                     HLVAKARAGGLLPEEYTGASFTISNLGSYGVEEFTAIINPPGSAILALGAVTAEPVVR
                     DGAVVVRRILHATLSSDHRVIDGALAAAFLADFKALCEEPARALL"
     gene            4171..5592
                     /gene="lpdA"
                     /locus_tag="PKI64_03875"
     CDS             4171..5592
                     /gene="lpdA"
                     /locus_tag="PKI64_03875"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOE00182.1"
                     /translation="MSHDFDVAVLGAGPGGYVAAIRAAQLGLKAAIIEKDKPGGVCLN
                     WGCIPSKALIHQADLVRSIPALQDLGVAVDTRKLDFAHAYKKSRAAAERLSKGIQFLL
                     KKNSIEYIKGEGRLTAPNEIALDGERRVRARAIILATGSRPRELPGLSFDETTALSST
                     GALNLKKLPPQIVILGAGAIGLELGYVWNAFGAEVTIIEGMNRILPLEDAEAAALVRK
                     LFEKRGIKFITGAFATAGARGPKGLALDLKPAPDHNGAEIPARVEANAVLVAIGRVPN
                     SANLGLAELGITLERGFVPVGDYCQTAARGVYAIGDLIATPQLAHVASKEGEIAAEHI
                     AATLLGSPLPRERRVDPTLVPSAIYCSPEIASFGLQEEQAKAKGIAHQVFSFPYRGIG
                     KAVATETADGFMKVVAAPGTGEILGAVIVGERATDLIHEPLLARGAELTTTDLADLVH
                     AHPTVAEGIMEAARGLLNRAIHV"
     gene            complement(5660..6076)
                     /locus_tag="PKI64_03880"
     CDS             complement(5660..6076)
                     /locus_tag="PKI64_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00183.1"
                     /translation="MWLDIKYEIEKSQAPEIVKLSLNIVTKAISWFPWIFVVIGFFLP
                     GSYFTLWLGFFLLVEGGLILALIYMLVLLVHDTLTHPPTGPISPEERREKESLAQAAL
                     KGIIGNIAFYIFILVAFIVIKSMGITDKITGIFLNK"
     gene            complement(6172..6990)
                     /locus_tag="PKI64_03885"
     CDS             complement(6172..6990)
                     /locus_tag="PKI64_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00184.1"
                     /translation="MKKEKIKTSLDAFISRLDSIQMTADVADDHKVASEKLHRCAKDI
                     VVFLNSNGYPSDAREVEAVMMHIEVTYGVPQIFRIKNDIDYYRTKLTVLRDALDEPTH
                     SSVEQVTESSEPVQRIGNSLDMPEKITLKWFMEHTPISFWPWVLGIIGMLFVAGIYVG
                     QLSFVKELIGRGTQETVLAEHLNTKRIVYTNVVQTGFIKSVAGPYVDVYYLNPFTGTP
                     ELTLIQATNGPDPAMAFEIFEQRPDGFRLSHFRHSDADTNGMIWKASGIRSVEE"
     gene            complement(7014..7445)
                     /locus_tag="PKI64_03890"
     CDS             complement(7014..7445)
                     /locus_tag="PKI64_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOE00185.1"
                     /translation="MKVRQISIFLENRSGRLASVLREVGRAGVNIRALSLADTSDFGI
                     LRLIVDDVDKGVQALRETGHTVSLTEVLAVEVPDHPGGLAGILDKLSAANLNVEYMYA
                     FVCRATEKAVVVFRFEDVDHAVATLQSSGISVLRAETVYQL"
     gene            complement(7461..8765)
                     /locus_tag="PKI64_03895"
     CDS             complement(7461..8765)
                     /locus_tag="PKI64_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE00186.1"
                     /translation="MMFNPTAEAMPRADLRKLQGERLQQLVQRAYHNVPFYRAKLDAA
                     GVKPGDIRRVEDIVKLPFTMKAEFRDQYPFGMFAVPQKDIVRIHASSGTTGKPTVVGY
                     TKYDIEVWAECAARSLACGGATAEDIVQVAYGYGLFTGGLGMHYGAEKLGAATLPMSS
                     GNTEKQIMLMKDFGVTVLCCTPSYALQVAEVAAQMGEDLMKLPLKSGHFGAEPWTEAM
                     RHRIEELFPLKALDIYGLSEVAGPGVANECLEQNGLHVFEDHFLPEIINPDTGEPLPD
                     GEKGELVFTCLTKEGVPLIRYRTRDITMILNEGTCRCGRTSRKIARLMGRTDDMLIIR
                     GINVFPSQVEDVLVRIEGVHPQYLIVVDRQGNLDTIEIKVEVTEALFSDEVKKLEALR
                     DRIQQQIRSVLSISAKITLVEPKTIERTLGKAKRVQDRRKLT"
     gene            complement(8840..9763)
                     /locus_tag="PKI64_03900"
     CDS             complement(8840..9763)
                     /locus_tag="PKI64_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HOE00187.1"
                     /translation="MALLLVAGCGLKEETARQPSAPPVLAPPVISGEAALAAVREFVE
                     LGPKDSGTPGAEQAAQYIFQQLQARGIAAEIQEFQDSTPDGELTFRNVLGRIPGGAGP
                     IVLLGAHYDTKIGIEGFVGANDSGSGVGLLLELARAFAAAEPLPLELRFAFFDGEECR
                     VRYGPNDGFHGSRYLAERMAADGSLSNVTAMIVLDMVGDAHLTVTIPRNSTPWLVSRV
                     FAAARAEDARQHFLLYPYPISDDHEAFFRRGVPAVDLIDFEFGSRPGANDYWHTAADS
                     MDKLSARSLDIVGRVVARMIVDLAATPAPGK"
     gene            complement(9992..10189)
                     /locus_tag="PKI64_03905"
     CDS             complement(9992..10189)
                     /locus_tag="PKI64_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00188.1"
                     /translation="MTLPGGSPGPQANIPAPWRLIIIIAGVLAIVLLGIMIRAALHYA
                     DEEKNQSIEEWSIEWSRNHPE"
     gene            11259..11891
                     /locus_tag="PKI64_03910"
     CDS             11259..11891
                     /locus_tag="PKI64_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOE00189.1"
                     /translation="MINLLRRLLGHNDKFFDLLETSAGEAQASVQHLSRMLRETEDVH
                     TIEELVHSRRHNKRITGEISQALCRTFVTPFEREDIEALSFALYKIPKSIEKFGEKFL
                     LGQPRGSLPGLDQQMALLGEAVGTVLEMVKRLRRHENLEVIQGLNDQLHQLEAKADRL
                     MMDMTHELYSGRHEALRVIIALDLFRTLEKVVDRCRDAGNIVFQMVLKYS"
     gene            11902..12963
                     /locus_tag="PKI64_03915"
     CDS             11902..12963
                     /locus_tag="PKI64_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOE00190.1"
                     /translation="MTLIFLACLVALAFEFINGFHDTANAIATSVSTKVLTPRQAITL
                     AASTNLLGALVGTSVAATIGTGLVDAAYVTPQTIFCALLGAVAWNLFTWWLGLPASSS
                     HALIGGLCGATLASADGRWAVIRWSARDAAGRLEGLWPKVIAPMIASPLLGIMGGLLV
                     MLALFGLLYRQRPHLINRVFGRLQVASAAFMGFSHGTNDAQKTMGILTLILFAGTQGG
                     ALDGLPAALGFLRTPVFRVAFWVKLLCALTMAAGTAMGGWRIIRTLGHKMVRLQPIHG
                     FAAETTAAAIIQMGSHWGIPLSTTHVITAAIMGVGATKRWNSVKWGLVGQMAWAWLLT
                     LPVTGLLGYALERALLLAR"
     gene            13079..15577
                     /locus_tag="PKI64_03920"
     CDS             13079..15577
                     /locus_tag="PKI64_03920"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOE00191.1"
                     /translation="MSAIAKQNVPAMEVPCNGKRLNSVEAIKCSYLNHLKYSLAKDEF
                     SATDHDRYYALALAIRDHLVDGWIRTSVTYHKTGVKRAYYLSMEYLIGRAMGNNVINL
                     QLDENVRKAMDDLGLSWDFLRDLERDAGLGNGGLGRLAACFLDSLATLQLPGYGYGVR
                     YDYGIFRQTVKDGYQVELPDNWLRFGNPWEIERPESQFEVHFGGRVEMEERNGRLNAR
                     WVDYETVLGVPYDLPIPGYGNNTVNNLRLWTAKSTEEFNLTFFNTGEYMKAYEAKTLT
                     ENITKVLYPNDNIEQGRDLRFKQQYFFIACSLHDILRRFRADGNEIHSFPDKVAIQLN
                     DTHPALATAELMRLLLDEEHLEWDDAWSIVLRTFGYTNHTLMPEALERWSVELFERIL
                     PRHLQIIYEINRRFLREIATRYPGDHDRIVRMSLIQEGPMREVRMAYLAVVASHSVNG
                     VAALHSELLKSTLFKDFYELWPERFNNKTNGITPRRWLLKANPGLAALITEKIGDGWI
                     TDLTQLKRLEQFAADRDFQKRVMAVKRENKVRLAELIHRELNVRVNVDSLFDVQVKRL
                     HEYKRQLLNALDIMHQYFELKSNPQMAFVPRTYIFSGKAAPGYFLAKLIIKLLNDVAA
                     VVNHDPAMKNRLNVLFLPDYRVSLAERIIPAADLSEQISTAGTEASGTGNMKFQLNGA
                     LTIGTLDGANVEIRQEVGAENFFLFGQTVEEVEATRRAGYNPWALYNANPALRRVLDA
                     IKNNFFNLDQPGLYQPIWDTLLTHGDYFQVLADFDAYVACQAEVAKTFEDKERWARMC
                     IMNIANSGKFSTDRTIAEYASEIWDIHPCPVNGD"
     gene            complement(15659..16030)
                     /locus_tag="PKI64_03925"
     CDS             complement(15659..16030)
                     /locus_tag="PKI64_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00192.1"
                     /translation="MTMPRRFQRTIRGLWAAGALGAALFVAAGCKTIHEEVTPLRETT
                     LTVARSGGEVTLSWIGTQGMFYSVMFAEARGAKAQWKLLPDAVNIRGMVTGEPIVVHD
                     RLPAAQQRYYRLHQGAKPLVP"
     gene            complement(16045..16683)
                     /locus_tag="PKI64_03930"
     CDS             complement(16045..16683)
                     /locus_tag="PKI64_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00193.1"
                     /translation="MKKIFWMIAVVALALSSVAVMPAAAQADAVLLVAPARFPVMQVA
                     FDVARRFPTILVSYQGSPDKPALHAWNGFEWMPLSLADYQSGAFLQAYPGRTIFLGDD
                     ALLPASLRGISAWCEQSTQIPELDNVGLINAIGNYLPFTPADWRWFAGRYNLSLTNLK
                     AEEAEAARRETWYGDKGAVKDPAPSFFKYFTRSRRHSAPAPVRIEPVEAETP"
     gene            complement(16764..18998)
                     /gene="hypF"
                     /locus_tag="PKI64_03935"
     CDS             complement(16764..18998)
                     /gene="hypF"
                     /locus_tag="PKI64_03935"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOE00194.1"
                     /translation="MAEEESILLRLRGRVQGVGFRPVVFCLAHELGLRGWVRNDGDGA
                     EVALAGPAAAIAAFRAALPGRLPAAAAITAVTEHSAPDDWPAGFEIRSSATPHGARAA
                     GILPDLATCPDCLREILDPRDRRFRYPFANCTHCGPRFSMIEALPYDRANTTMRGFTL
                     CADCAAEYADPRNRRFHAQPNACPVCGPHLTWTAADGTVQTTHDAALLAAAAAVRAGR
                     IVAVKGLGGFHLMVAAHDAAAVQRLRARKRRDEKPFALMFPSLAAIQAVAEVSAAEQT
                     WLESAAAPIVLLRKRAATPGIAEEVAPRLPWLGAMLPYTPLHHLLLREIGFPVVATSG
                     NLSDEPICIDNEEARERLGDIADFFLLHNRPIARPMDDSVMAVVDEQPVVLRRGRGLA
                     PYSLPVAGAPDGLVAAGAQMKGALAVAVGGNAVMSPHLGDLDHDGAARLWARTARDLT
                     ALHGLRITRAAVDLHPDYAATRGAAEWGVPLTAVQHHHAHIAACLAENQLAGPALGIA
                     WDGTGLGPDGTIWGGEFLVCTRAEYRRAAWLRAFPLIGGDAAAREPRRVALGLLREMG
                     APLPELGFSAQEIAVLDGMLRENVNVARTSSAGRLFDAVAALLGICHVMAHEGQAAMR
                     LEALAGDLAAEPYPFGWRGTELDWAPMITALLAGGEPPATAAARFHETLVAMMVAVAQ
                     REGLADVCLSGGCFQNRRLLATATHALTRAGFRVWRHREIPPNDAGICLGQLVVASAQ
                     AAEH"
     gene            19094..19489
                     /locus_tag="PKI64_03940"
     CDS             19094..19489
                     /locus_tag="PKI64_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOE00195.1"
                     /translation="MRRRKPWHKGKWQISRERFQIADETPPLEDPGIQSIGAFIPAVL
                     NGLKLDDHAQDAAIVAAWPELVGPQLAANTRPANLANRVLTIFVSHPGWLMELRGPLS
                     AEILTRLQNKFGAHCIKSIRLAIDPKPRR"
     gene            19551..22412
                     /locus_tag="PKI64_03945"
     CDS             19551..22412
                     /locus_tag="PKI64_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE00196.1"
                     /translation="MEKTTGYFPQCGKFARHFSTVWKTFFHSVENPAAARKKIPRHFF
                     RAIASALLLLAAAAPASAGWIAQTSNQVTLNLTLFNLPDPSRTDPATRAELVVQREFL
                     RLAPDLLRARQAADPARYGALDLRNLAVQLHRFSGIQVEGIESTLLAIAGNVAPDVLY
                     VNFRQSETYISQGFLYPLDRPTDGYATALPPAEWARRVHPKIEPVIRRPGPDGAVHVW
                     AMPSGPPLGRVVLYRRDLFEAAGLPAPQPDWTWDDFYRVCRQVADPARGIYALGLSRG
                     KHESYLWLPFLWGAGGEALVFAEQENRWRAVFDSPAAATALDFYVRLTTEPWTDAEGK
                     RRRGYVIKDTAESNLKWRRGQLAMMFAYIDDKLLAGLNPDLTGIAPLPIGPVTRGTEI
                     NSRMMGIFAGVTHPLVRDAAWEYINFQYSPAAEAIRIQTLVAGGLGRFLPPEQLRAQG
                     YEHIVGTIPAEWRDSHAIAIAAGRPEPYGKNANVIYDILTTPIRQAEELSLAGRLATD
                     PAARQAQLRELLVQARRKADADMLGVVPPAELAKRRTAASALLLVILASVLLVLRHMA
                     RLFLPAPATRPAPGAAPPRPRATGGWLRRHWRALLLLFPAAATIAVWGYVPLVRGAAM
                     AFFDYRIFGDSTWVFLDNFADLLWDPDWWRALLTSARYAVLVITLTFIPPVLLAILLQ
                     EVPRGRIAFRLIFYLPAALTGLVVILLWKTFYEPSEAGLLNRLILPIPAGGWLLLSAG
                     ALLVALHLARRLLRHQLWRPALFTVAAGLLLAAAPLGPLREIMRTATTGHSFVSLFAT
                     LPEPVRWLDDSRTALLACVLPMLWAGMGPGCLIYLAALKGIPDELYEAADMDGASFTD
                     KILFVVIPTLRPLLIINFVGVFIAAWQAEANILAMTAGGANTEVAGLHIFYKAFIFLR
                     LGPATAAAWMLGCLLVGFTLYQLRILSRVEFRANREGTA"
     gene            22415..>23718
                     /locus_tag="PKI64_03950"
     CDS             22415..>23718
                     /locus_tag="PKI64_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00197.1"
                     /translation="MPILAKIGRKSWGTRLRIGAMYSALAIGALAMLYPLSLMLAGST
                     KSIADQRDNVLLPRMLVSDTALWQKHLEALFNESLDALNIAFDTDYPSFESIPLPAPN
                     AAPGADLVPLWREFLATGALTPESIALGHYWAPQAGAFPVQLREFRRHLRAKFGDLDA
                     LNTALGADFAAWYAVFIQPPAYLFPHTVPAATPLAAEFDSFKLNAPPWCQTVLSPAGF
                     FHKLYLKPRYSADIAAYNAAHGTAYAAYADIPLPRTCPADAPPLVQEDWRDFTQYSLA
                     PLWLVDGALDTPETRWHDWLARAHPSVIAPGSSVPPMPQLAHHVQLFREHRAAIRHEL
                     LWRNYRTVLDYILFHGRGILNTTIYCALAVLLALLVNPLAAYALSRFKPAVGPQILLF
                     LMLTMAFPHMVTQIPVFVLLRELGLLNTFAALLLPGMASGYS"
BASE COUNT         4319 a         7885 c         6938 g         4476 t
ORIGIN      
        1 tgcccgaccg cgggcggctg ttggcggcgg tattgacggt gctgcacgag ctgggcatcg
       61 cggcggaaga aggaccacgc ggcggggtgt atgtggccgg gcgcaaaatc ggcggcacag
      121 cggcggcggc gggaaaaggc gttttgctgt ttcatggcac gctgctcgtc actaccgatt
      181 tgacggcgct caccgcggca ttggcggcgc attcgccgga atatggcggc gaaacgggcc
      241 acgccgcgcg cgcgggcgtg gcttcggtgc cctcccccgt gaccacgctt gcccacctgc
      301 gccccggatt ggatgtggcg acggtggaag ccgcgctttt cgcagggctc gcgcgcgccg
      361 gcgccccgca gccgccggag acgtttctgg atttgagcaa cattcgtttt ttggccacgg
      421 actacggccg ggagtcctgg attttgaacc gtacgccgcc ggcccggcag acgagaggag
      481 tcccatgaaa gaaaaagacc ccctgcgagc cctccgggcc ttgttggata ccattcgggt
      541 gggcgtgctg tgcaccgtca gcaaggatgc ccagccgcgc tgccgctgga tgacagtggc
      601 cgccctgccg cgcgtgcagg gctgcttcta ctgcgtaacg agcaaaggct cggccaaggt
      661 gaaggaaatc gaagccaacg cgcgcgtgca atggacgttc caaacgccga cgctggatga
      721 aatcatttcc gtgtgcgggc aggcggaagt gctgcaaaat cccggcctga aagccgaggt
      781 tctcgaagcg ctcggcccca acctgaccat cttctggcgc gtgaatccgg acccggccaa
      841 gctggttgtc attgagacgg tggtggacaa cgccaagctg ttccggccca tggaatgcct
      901 ctatgtggag aaggaggccg gcacatgaag gagttgaatg gcgaactgct ttgggagctc
      961 cgcaacaaca tggtgttgat gcgggtcttc gaagagaagg ccgggcagat gtacggcctg
     1021 cgcaaaatcg ggggcttttg tcacttgtat aacgggcagg aggccatcgc cgccggtttc
     1081 ggcagcgtgc tggactacag ccgcgattat gtactgaccg gctaccgcga ccacggccac
     1141 gcgctggcgg cgggcatgga gccgcgcgcc atcatggccg agctgttcgg caaagtgacc
     1201 ggctgctcgc gcggcaaagg cggctcgatg caccttttcg atgcgcggcg ccatttcttg
     1261 ggcggcaacg gcattgtggg cgcgcagatt cctgtggccg ccggcgtggc gtttgcccag
     1321 aagtatttga aaaccggcgg cgcgaccgtc gttttttttg gcgatggcgc catccatcag
     1381 ggcgcgttcc acgaggccat gaacctcgcc aaaatttgga acctgccgct ggtgtcggtg
     1441 tgcgaaaaca accagtgggg catgggcacc agttggaagc gcgtgtcgtc ggtggatgat
     1501 ttttccgtca tggccacagc ctacggaatg cgcggcgaac tggtggatgg catggatgtg
     1561 ctggaggtgc gcgacgccgc cgggcgcgcg ctggccgaag cgcgcgaagg ccaccccgtg
     1621 ctgatcgagg cgcgcaccta ccgctataag ggccactcca tgagcgaccc gcaaaaatac
     1681 cgcacgcgcg aagaagttga aaaatacaag cgcgagcagg accccatcct gattctggaa
     1741 accctcatga aggaagccgg ccactgggat gacgcccgcg ccgccgcaca ggaccgcgcc
     1801 gccgaggaaa tctcgaagga gtccgtggac tttgccgagc gctccgccga gcctgcgctg
     1861 cacacgctct atgaggatat ttacgccggc gagccattct tcatggcgcc gtccaacccc
     1921 gccaccctcg tcacctgaac aggagcacgc catgtcgcaa nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn gtggaatgga tgacccacaa ctttgcgctg ctggcgctgg
     2101 accaggtggt gaacgaagcc gccaaaatgc ggcacatgtc cggcggccaa ttccacgtgc
     2161 ccgtggtgtt ccgcggcccg aatggcccgg cggaatatct ctccagccag cactcacagg
     2221 ccctcgccag ctactgggtg cacgtgcccg gtatccgcgt ggtagcgcct gcttttccgg
     2281 ccgatgccta cggcctgctc aagagcgcca tccgcgatcc cgaccccgtc gtggtcctcg
     2341 aggcggaaat gctctacggc ttcaaggccg aagtgcccga tgaagaattc ctgatcccta
     2401 tcggcaaggc caacacgttg cgcaacggca atgacatcac actggtgtcg ttcggcaagc
     2461 cggtgcacct gctgctgcaa gccgccgatg aactcgcccg cgacaacatt gctgccgagg
     2521 tgattgacct gcgcagcctg cgcccgctcg acgaagaaac cctcatggcc agcgtggccc
     2581 gcaccggccg tgctgtggtg gtggatgaat cgtggccggc cgccagcgtc ggctcctatg
     2641 tggcccaccg catcgccacc gcgtgcttcg acttgctcga cgcgcctgtg gaattcgtgg
     2701 ccagcgaaga cgtgcccatg ccctacaacc acaccctcga actggccgtt caacccagcg
     2761 tcaaaaaaat tgtggccgcc gcccggcgcg ccctctatct ggaggttcct catggctgat
     2821 cctgtcctga tgattgcgct gtccccgacc atgaacgagg ggaccattgt ccaatggttt
     2881 gcccaggagg ggcaggagat caaaaccggc gacgcgcttt gcgaggtgga aaccgacaag
     2941 gccaccatga cctatgaagc gccgtcggcc ggcacgttgt tgaagatcat cgcggggccg
     3001 ggtacttccg cggtggtggg ccaactgatt gcggtggtcg gcaagcccgg cgacaactgg
     3061 gaagaaattg ccgccaaggc acaagccgcc gctcccgccg cgccggcgca acccgcggcg
     3121 cctgctgcgg caccccccgc cccccctcct ccccccgccc ctgcgcccgc gcctgctccg
     3181 gcagtcgccg cgccggtggt ggccagtgtg tcgggtgccg ccccgcaact ggcgccgggc
     3241 gtgccgcgtt catcgccggt ggcccgcaaa ctggcacagg aaaaagggct cgatttgcgc
     3301 ggcatccgcg gcagcggccc cggcgggcgc atcatcgcgc gcgatgtcga acaagcccac
     3361 gcggcaccgg ctcccgccat ggcggcagtg gcaccgcggc ccgccggcag cgcgcccgcg
     3421 gcggccacgc cgcaatcccg cggcttgcgt ggcgaccgcg tgccgctttc caaaatgcgc
     3481 agcatcattg ccacgcgcct gcaccaaagc tattcgcaag ccccgcacta cttcgtgcgc
     3541 gtggccatcg agatggaccg cctgttggac ctgcgcaagc agctcaacgc gggccgcgac
     3601 ccggcgctct cgctcaacgc attcatcgtc aagctggccg ccgccgcgct ggaacgcaac
     3661 cccggcatca acgccagttg ggaaggcgat gccattcaat atctgccaca tgccgatatc
     3721 ggtttggccg tcgcgctgcc cggcgggctc atcacccccg tgattcgcgc ctgcgaagcc
     3781 aaagggctcg aggccattga tcaggacgta ggccacctgg tggccaaggc gcgcgcgggt
     3841 gggctgctgc cggaggaata taccggcgcc agcttcacca tcagcaacct cggctcttac
     3901 ggcgtggaag aattcaccgc catcatcaat ccgcccggtt cggccatcct cgcgctcggc
     3961 gcggtgaccg ccgagcccgt ggtgcgcgat ggcgcggtgg tcgtgcgccg catcctgcac
     4021 gccacgctgt cctccgacca ccgcgtgatt gatggcgccc tagccgccgc gttcctcgcc
     4081 gacttcaagg ccctgtgcga agagcccgcg cgggccttgc tataaggaag cccgcggcgc
     4141 acaatcaccc gcccatgaaa ggaatccgcc atgagtcatg attttgatgt tgccgtgttg
     4201 ggcgccggcc ccgggggcta tgtggccgcc atccgcgccg cgcagttggg cctcaaggcc
     4261 gctatcatcg aaaaagacaa gcccggcggg gtatgcctca actggggctg catcccgagc
     4321 aaggcgctga ttcaccaggc cgacctcgtg cgcagcattc cggccttgca ggacctcggc
     4381 gtggcggtgg atacgcgcaa gctggacttt gcccacgcct acaagaaaag ccgcgcggcg
     4441 gcggagcgtc tttccaaggg catccagttc ctgctgaaaa agaattccat tgaatacatc
     4501 aaaggcgaag gccggctgac cgctcccaac gagattgcgc tggatggcga gcggcgggtc
     4561 cgcgcccgcg ccattatttt ggccaccggc agccgcccgc gcgagttgcc cgggttgtcc
     4621 ttcgatgaaa ccaccgcgct ctccagcacg ggcgcgctga acctcaaaaa actgccgccg
     4681 cagattgtca ttcttggcgc aggggccatc ggcctggagc tcggctatgt ctggaatgcc
     4741 ttcggcgcgg aagtcaccat catcgagggc atgaaccgca tcctgccgct ggaagacgcg
     4801 gaagccgcgg cgctggtgcg caagctgttt gaaaagcgcg gcatcaaatt cattaccggc
     4861 gcgtttgcca ccgccggtgc gcgcgggccc aagggcctgg cgctcgacct gaaacccgcg
     4921 cccgaccaca acggcgcgga gattcccgcc cgagtggaag ccaacgcagt gctggtggcc
     4981 attggccgcg tgcccaacag cgccaacctc ggcctcgcag aactcggcat caccctcgaa
     5041 cgcggctttg tgcccgtggg cgattactgc caaaccgccg cgcgcggcgt gtacgccatc
     5101 ggcgacctga tcgccacccc gcaactggcg cacgtcgcca gcaaggaagg cgaaattgcc
     5161 gccgaacata ttgccgccac gctgctgggc tcgcccctgc cgcgcgaacg acgcgtggac
     5221 cccacgctgg tgccctccgc catctattgc tcaccggaaa ttgccagctt cggcctgcag
     5281 gaagagcagg cgaaagccaa gggtatcgcg catcaggtat tcagtttccc gtaccgcggc
     5341 attggcaagg cggtcgccac ggaaacggcc gatggcttca tgaaggtggt cgccgctccc
     5401 ggcacaggcg aaatcctcgg tgcggtgatc gtgggcgaac gcgcgacaga tttgatccac
     5461 gagccgctgc ttgcgcgcgg cgcggagctg accacaacgg acttggccga tctggtccac
     5521 gcccacccca ccgtggccga aggcatcatg gaagccgccc gcggcctcct caaccgcgcc
     5581 atccacgtgt aagaggccac gttacggctg agcggcggtg gtacgccgcg cgctcaagcc
     5641 gctgattggg tgctttctcc tatttgttca ggaagattcc ggtgatcttg tctgtgattc
     5701 ccatcgattt gatcacaatg aaggctacga gaatgaagat gtaaaatgct atgttcccga
     5761 taattccttt gagcgcggcc tgagcaaggc tttccttttc ccgtctctct tccggtgata
     5821 tcggcccagt gggcggatgt gtgagcgtgt catggacgag aagaacgagc atgtagatga
     5881 gggcgaggat gagtcctcct tcgacaagga ggaagaagcc cagccagaga gtgaagtagg
     5941 atccagggag gaagaagcct atgacgacga atatccaagg gaaccaggag atggccttgg
     6001 tgactatgtt gagggacagc ttgacgatct ctggggcctg cgacttctcg atttcgtact
     6061 ttatgtcgag ccacatgatt cacctctgtg ttcctgcacc caatagcacg ggccaagggg
     6121 tgacacatct tgtgcggtgt actccacaac cgattgttgg atcctgacgc ttcattcttc
     6181 aaccgatctg ataccagaag ccttccagat cataccattt gtatcagcat cactgtgcct
     6241 gaaatgactc aaacgaaaac catcggggcg ttgttcaaag atttcaaacg ccattgctgg
     6301 atccgggcca tttgtggctt ggatgagcgt tagctcaggg gttccagtaa aaggattgag
     6361 gtaatagacg tcgacatagg gtcccgctac agatttgata aacccggttt gtactacgtt
     6421 cgtatataca atgcgcttgg tattgaggtg ctcagccaaa acagtctctt gggtgcctct
     6481 accgattagt tccttcacaa acgagagttg cccgacgtaa attccagcca cgaatagcat
     6541 cccaatgatc ccgagcaccc acggccagaa tgaaatcgga gtatgttcca tgaaccattt
     6601 cagcgttatt ttctctggca tatccaggga gttacctatg cgttgaacag gctcagatga
     6661 ctctgtgact tgttcgactg aagagtgtgt aggttcatca agcgcatcac gcaaaaccgt
     6721 taatttcgtt cggtaataat ctatgtcatt cttgatgcga aagatttgag gtacgccata
     6781 ggttacttca atatgcatca ttacagcttc gacttccctc gcatcgcttg ggtatccatt
     6841 ggaattgagg aataccacta tatccttagc acaacggtgg agtttttccg atgcgacctt
     6901 gtggtcatca gcaacatcgg cagtcatctg tattgaatcc aaccgtgaaa tgaatgcgtc
     6961 gagggatgtt tttatttttt ctttcttcac attatgtagt gcccaacagc aaattagagc
     7021 tggtacaccg tctcggcgcg caggacgctg atgccggagc tttgcagcgt ggccacggcg
     7081 tggtccacat cctcaaaccg gaagaccaca accgcttttt ccgtggcgcg gcacacaaac
     7141 gcgtacatgt attccacgtt gaggttggcg gcggaaagct tgtcgagaat gccggccagc
     7201 ccgccgggat gatcgggcac ttccacggcc agcacttcgg tgagcgacac ggtgtggccc
     7261 gtctcgcgca gcgcctgcac gcctttgtcc acgtcatcca caatcagccg cagaatgccg
     7321 aagtcgctgg tgtcggccag cgagagggcg cgaatgttga ccccggcgcg gccgacctcc
     7381 cgcagcacgc tggcgagccg ccccgaccgg ttttccagaa aaatcgagat ttgccgaact
     7441 ttcatgttgc cgctccttgc ttaggttagt ttgcgccggt cctgcacccg cttggctttg
     7501 ccgagggtgc gctcaatggt cttgggttcc accaacgtaa tcttggcgct gatgctgagc
     7561 accgagcgaa tctgctgctg gatgcgatcg cgcaacgcct cgagtttttt gacttcgtcg
     7621 ctgaagagcg cctcggtgac ttccaccttg atttcgatgg tgtcgaggtt gccctgccgg
     7681 tccaccacaa tgaggtattg cggatgcacg ccctcgatgc gcaccagcac gtcttccacc
     7741 tgcgacggga atacgttgat gccgcggatg atgagcatgt cgtccgtgcg gcccatcagg
     7801 cgcgcgattt tgcggctggt gcggccgcag cggcaggtgc cttcgttgag gatcatagtg
     7861 atgtcgcgcg tgcggtagcg gatcaggggc acgccttcct tggtgaggca ggtgaaaacc
     7921 agctcacctt tttcgccatc gggcagcggc tcgccggtgt cggggttgat gatctccggc
     7981 aagaagtggt cctcgaacac gtgcaggccg ttttgctcca ggcactcgtt ggccacgcct
     8041 gggccggcca cttccgagag gccgtagata tccagcgcct tgagcgggaa gagttcttcg
     8101 atgcggtggc gcatggcctc ggtccacggt tccgcgccga agtgcccgct cttgagcggc
     8161 agcttcatca ggtcttcgcc catttgcgcg gccacctcgg ccacttgcag cgcgtagctc
     8221 ggcgtgcagc acagcaccgt cacgccaaaa tccttcatca gcatgatttg cttttcggtg
     8281 ttgccggagg acatcggcag cgtggccgcg ccgagttttt cggcgccgta gtgcatgccg
     8341 aggccgccgg tgaagaggcc gtagccgtag gccacctgca caatatcctc cgccgtggcc
     8401 ccgccgcacg ccagcgagcg cgccgcgcat tccgcccaca cttcaatgtc gtacttggtg
     8461 tagcccacga cagtcggctt gccggtggtg ccggagctgg cgtggatgcg cacaatgtct
     8521 ttttgcggca cggcgaacat gccgaaggga tactggtcgc ggaactccgc cttcatggtg
     8581 aagggcagct tgacaatgtc ctccacgcgg cggatatcgc cgggcttcac gccggcggca
     8641 tccagtttgg cgcgataaaa cggcacattg tggtaagccc gctgaaccaa ttgctggagc
     8701 cgttcgccct gcaatttccg caggtccgcg cgcggcatgg cttcggctgt tggattgaac
     8761 atcatggcgc tccttgatct tgcatgggcc atgctggcct tgcggccccg cgcctgtcca
     8821 gcccaaaccg cccgtggcgt tattttcccg gcgcgggcgt cgcggccaaa tccacgatca
     8881 tccgcgccac cacgcgaccc acgatgtcca gactgcgggc ggaaagttta tccatgctgt
     8941 ccgccgcggt gtgccagtag tcgttggcgc cggggcggct gccgaattca aaatcaatca
     9001 ggtccaccgc cggcacgccg cggcggaaaa acgcctcgtg gtcgtcgctg atgggataag
     9061 gatacaggag gaaatgctgc cgcgcgtctt ccgcccgcgc cgccgcgaac acccgcgaca
     9121 ccagccacgg cgtgctattg cgcggaatgg tcacggtgag gtgggcatcg cccaccatgt
     9181 ccagcacaat catggccgtc acgttcgaca aactgccatc cgccgccatg cgttcggcca
     9241 aataccggct gccgtggaag ccatcgttgg ggccataccg cacccggcat tcttcgccat
     9301 cgaagaacgc aaaacgcaac tccagcggca gcggttcggc cgcggcaaac gcgcgcgcca
     9361 gttccagcaa caggcccacg ccggagcccg aatcattcgc gccgacaaag ccctcaatgc
     9421 cgatcttggt gtcgtagtgc gcgcccagca gcacgatggg ccccgcgcca cccggaatcc
     9481 gccccagcac attgcgaaag gtcagttcgc catccggtgt tgagtcttgg aactcctgaa
     9541 tctccgccgc gatgcctcgt gcttgcagtt gctgaaaaat gtactgtgct gcctgttccg
     9601 cgcccggcgt gcccgaatcc ttggggccca attccacaaa ctcgcgcacc gccgccaagg
     9661 ccgcctcgcc ggagatgacc ggcggcgcca gcaccggggg cgctgaaggc tggcgtgccg
     9721 tttcttcctt gaggccgcag cctgccacca acagcaacgc cacccaccag caacaccacc
     9781 ggaacactcc gtccctgcgg cggacacaaa agcccgcacc cggccaccgc ttcggcctcc
     9841 cgttgctctg tatattgact aagtccgttt ccaccgtcat cggctcctgt caaatccgcg
     9901 accctcccct tctacgggaa ctccccgccg acctcaaggc tgcgttgcca ccttcggaaa
     9961 ctttgtggcg catgaacgcg gcgtgctccg ttcattccgg atgattccgg ctccattcga
    10021 tggaccactc ctcgatactt tggttcttct cctcatcggc gtaatgcaaa gcggcccgaa
    10081 tcatgatgcc caggagcact atggcgagca ctccagcgat gataatgatc aatcgccaag
    10141 gagcgggaat gttggcctgc gggccagggc tgccaccggg taaggtcata ttggttccct
    10201 atatccttaa aatgaccaca aaagtcgaga ctctggcggc atccgcctct acaatccaac
    10261 ccgaatgcgg ccatggctgt tcttgccgga attttgtagg gcgagaatgg ttgaggaacg
    10321 aaaccttccc gccgggaacg gctggaaggt ctgcgaccat tccatcccta caatccagcc
    10381 ggaatcggtt tttagggccg aggcaccaca aaatgaaatc gcccggttgc aacgcaacgc
    10441 gtgacaacaa gatttttgtg gagcgcggaa ctcaggccaa ttttgccatt tttatggcat
    10501 ttttggccgt ttttacgcga ttttagccaa atttggcctg ttttttgcgt ttttgtccgg
    10561 gtttcggcgg ttttttggcg atttgggccg attttttcca tttttttgat tgatcaaagg
    10621 gccaccttga atgcgccaat tttgcagaaa aactggtgag ccgcaactgt cctcaaaccg
    10681 acctcttttt ttgctatttt ggacgtgatg tccagagttt ataaactgtg gacacttttt
    10741 ataatgttta tatctctatt atatacaact tattatgtat attttttcgc atatttttaa
    10801 tcggcagttt tacaggcaat gtccagagtt tataaagtgt ggacagttca gaagtgaggt
    10861 tttttgtgtt ttcccccacg ctataagagg cgcgattgac aagaattttg acgccccggg
    10921 tcacctgcag ggatttcagg gcaaccgtcg ctgccatggc gttgttatgg tgttcctgag
    10981 gtcaatggcc accgccaatc cacaaaaccg acgcgacaat tggcgcttgg ccaaaattta
    11041 ctgtttttgg ggtgtttttg gccatttttt cgtgaatttg gccgattttg gccgattttt
    11101 tgcattttta gccagttttt ggcgattttt tggccaaaac cggggaatta tgcgcttggt
    11161 ggcgccggcc ccgacaaatg gataatcggc aaaacagcca ataaaaaacg ctttacttta
    11221 tacttgcgcc cggcagggct tctgctaggc tgccgggcat gatcaatctg ttgcgccgac
    11281 tgttgggtca taacgacaag ttcttcgacc tgctggaaac cagcgccggt gaggcgcagg
    11341 ccagcgtgca gcatctgtcc agaatgctgc gggaaacgga ggatgttcac accatcgagg
    11401 aactggtgca tagccgtcgc cataacaagc gcatcacggg cgaaatttcc caggctctgt
    11461 gccggacctt cgtcactccg ttcgagcgcg aggacatcga agcgctttcg tttgcgctct
    11521 acaaaatccc caaatccatc gaaaagttcg gcgaaaagtt cctgctgggc cagccgcgcg
    11581 gctccctgcc cggccttgac cagcaaatgg cgcttctggg cgaggctgtg ggcaccgtgc
    11641 tggagatggt gaaacggcta cgtcgccacg aaaatctgga agtcattcag gggctcaatg
    11701 accaactgca ccagctcgag gccaaagccg accggctgat gatggacatg acgcatgagc
    11761 tctacagcgg ccgccatgag gcgttgcggg tcattattgc cctcgatttg ttccgcacgc
    11821 tggagaaggt ggtggaccgc tgccgggatg cgggcaacat tgtttttcaa atggtgctga
    11881 aatactcctg accggccgcg catgacgctg atttttctgg cttgtctggt ggcgctggcc
    11941 tttgagttta tcaacggctt tcacgacacc gccaacgcca tcgcgacgag cgtttccacc
    12001 aaagtgctga cgccgcggca ggccatcacg ctggcggcga gcaccaatct gctgggggcg
    12061 ctggtgggca cgtccgtggc ggcgaccatc ggcacggggc tggtggatgc ggcctacgtc
    12121 acgccgcaaa ccattttttg cgcactgctg ggcgcggtgg cgtggaacct gttcacgtgg
    12181 tggctggggt tgccggccag ttcaagccat gcgctgattg gcgggctgtg cggcgccacg
    12241 ctggcttccg cggatggccg ctgggcggtg atccgctggt cggcgcgcga tgccgccggc
    12301 cggctggagg ggctctggcc caaggtcatc gcgccgatga tcgcctcgcc gctgctgggg
    12361 atcatgggcg ggctgctggt gatgctggcg ctgttcgggc tgctgtaccg ccaacgccca
    12421 cacctcatta accgcgtgtt cggccgcttg caggtggcca gcgccgcgtt catggggttt
    12481 agccatggaa ccaacgacgc ccaaaaaacc atgggcatcc tcacgctgat cctgtttgcg
    12541 ggcacgcagg gcggggcgct ggatggcctg ccggcggcgc tcggtttttt gcgcacgcca
    12601 gtttttcgcg tggcgttttg ggtcaagctg ctctgcgcgc tgaccatggc ggcggggacc
    12661 gccatgggcg ggtggcgcat catccgcacg ctggggcaca agatggtgcg cttgcaaccc
    12721 atccacggtt ttgcggcgga aaccacggcg gcggccatca tccagatggg cagccattgg
    12781 ggcattccgc tttcgaccac gcacgtcatc acggcggcga tcatgggcgt gggcgccacc
    12841 aagcgatgga actccgtcaa atggggactg gtggggcaaa tggcgtgggc atggctgctc
    12901 acgctgccgg tcaccggcct gctgggctac gcccttgaac gcgcgctgct gctggcccgc
    12961 tgatgccgcg cgcggggcgg ctttgtttcc ttgacctgcc gcaagtgaat ccctactttc
    13021 cccgaaacgg tttaccccgt ggtgtgggtt gccgcagttt gattgcagga gtttttggat
    13081 gagtgcgata gccaaacaga atgtgccggc catggaagtg ccgtgcaatg gcaagcggtt
    13141 gaatagcgtg gaagccatca agtgcagcta cctcaaccac ctgaagtatt ccctcgccaa
    13201 ggacgagttt tccgccacgg accacgaccg ctattacgcc ttggcgctgg ccatccgcga
    13261 ccacctcgtg gacggctgga tccgcacctc ggtcacctat cacaaaaccg gcgtcaagcg
    13321 cgcctattac ctctcgatgg agtacctcat cgggcgcgcc atgggcaaca acgtcatcaa
    13381 tttgcaattg gatgaaaacg tccgcaaggc catggacgac ctcggcctgt cgtgggactt
    13441 cctgcgcgac ctcgaacgcg atgccgggct gggcaacggg ggcctcggcc ggctggcggc
    13501 ctgtttcctc gactcgctcg ccacgctgca actgccgggc tacggctacg gcgtgcgcta
    13561 cgactacggc atcttccgcc agaccgtcaa agatggctat caggtggagt tgcccgacaa
    13621 ctggctgcgt ttcggcaacc cgtgggaaat cgagcgcccc gaatcgcaat tcgaggtcca
    13681 cttcggcggg cgcgtggaaa tggaagagcg caatgggcgg ctaaacgccc gctgggtgga
    13741 ttatgaaact gtgctgggcg ttccctacga cctgcccatc cccggctacg gcaacaacac
    13801 cgtcaacaac ctgcgcctgt ggacggccaa gtccaccgag gaattcaacc tcaccttctt
    13861 caacaccggc gagtacatga aggcctacga ggccaaaacc ctcaccgaaa acattaccaa
    13921 ggtgctgtat cccaacgaca acattgagca ggggcgcgac ctgcgcttca agcagcaata
    13981 cttcttcatc gcctgctcgc tccacgacat cctgcgccgc ttccgggccg acggcaacga
    14041 aatccattcc ttccccgaca aggtggccat ccagctcaac gatacccacc ccgccctcgc
    14101 cactgccgag ctgatgcgcc tgctgctgga tgaggagcat ctggagtggg acgacgcatg
    14161 gagcattgtc ctgcgcacct ttggctatac caaccacacc ctcatgcccg aggcgctgga
    14221 acgctggtcc gtcgagctct tcgaacgcat tttgccgcgc cacctgcaaa tcatctacga
    14281 aatcaaccgc cgcttcctgc gcgaaatcgc cacgcgctat ccgggcgacc acgaccgcat
    14341 tgtgcggatg tcgctcattc aggaaggccc catgcgcgaa gtccgcatgg cctatctcgc
    14401 cgtggtggcc tcgcactctg tcaacggcgt ggccgccctg cactccgagc tgctcaagtc
    14461 caccctcttc aaggatttct acgagctgtg gcccgagcgt ttcaacaaca agaccaacgg
    14521 catcaccccg cgccgctggc tgctcaaggc caaccccggc ctcgccgcgc tcatcaccga
    14581 aaaaatcggc gatggctgga tcaccgacct gacccagctc aagcgcctcg aacaatttgc
    14641 cgcggaccgc gacttccaaa agcgcgtcat ggcggtcaag cgcgagaaca aggtccggct
    14701 ggccgagttg attcaccgcg agctcaacgt gcgcgtgaac gtggactccc tcttcgatgt
    14761 ccaagtcaag cggctgcacg aatacaaacg gcagctgctc aacgcgctgg acatcatgca
    14821 ccagtacttc gagctgaaat ccaacccgca gatggcgttc gtgccgcgca cctatatctt
    14881 cagcggcaag gccgcaccgg gttacttcct cgccaagctg atcatcaagc tgctcaacga
    14941 tgttgccgcc gtggtcaacc acgacccggc catgaagaac cggctcaacg tcctcttcct
    15001 gcccgattac cgcgtctcgc tcgccgagcg catcatcccc gccgccgacc tgtccgagca
    15061 gatttccacg gccggcaccg aagccagcgg caccggcaac atgaagttcc agctcaatgg
    15121 tgcgctgacc atcggaacgc tcgatggcgc caacgtcgaa atccggcagg aagtcggcgc
    15181 ggagaacttc ttcctgttcg gccagaccgt ggaagaagtc gaagccaccc gccgcgcggg
    15241 ctacaacccg tgggcgcttt acaacgccaa ccccgcgctg cgccgcgtgc tcgacgccat
    15301 caaaaacaac ttcttcaacc tcgaccaacc cggtctgtac cagcccatct gggatacgct
    15361 gctcacccac ggcgactact tccaggtgct ggctgatttc gatgcctatg tcgcctgtca
    15421 ggcggaagtg gccaagacct tcgaagacaa ggaacgctgg gcgcggatgt gcatcatgaa
    15481 tattgccaac tccggcaagt tcagcaccga ccgcaccatc gctgaatacg ccagcgaaat
    15541 ttgggacatc cacccctgcc ccgtcaacgg cgactgagcc ccggcaaaac ttgcggcgta
    15601 aaacattcgg cgcgcggcgc gtgccttccc ccgtaaattc attggccgcg ggcgcggcct
    15661 acggcacgag cggcttggcg ccctgatgca ggcgatagta gcgctgctgc gccgccggca
    15721 gacgatcatg taccacgatc ggttcgccgg tcaccatgcc gcggatattg actgcatccg
    15781 gcagcaactt ccattgtgcc ttcgccccgc gcgcttcggc aaacatgacg gaataaaaca
    15841 tcccctgcgt gccgatccac gaaagcgtca cctcgccacc ggagcgcgcc accgtcagcg
    15901 tggtctcacg caacggggtc acttcttcat ggatggtctt gcaaccggcc gcaacgaaca
    15961 gcgccgcccc caacgcgccc gcggcccaca ggccccggat cgtgcgctgg aaacggcgcg
    16021 gcatggtcat gaaccgctgg agccttacgg cgtctcggcc tcaaccggct caatgcgtac
    16081 cggggccggc gccgagtggc ggcgcgaacg ggtgaaatac ttgaagaacg acggcgccgg
    16141 atccttgacc gcgcctttgt cgccgtacca cgtctcgcga cgggcggctt ccgcttcttc
    16201 cgccttcaag ttcgtcagcg aaagattgta gcggcccgca aaccagcgcc aatccgccgg
    16261 cgtgaagggc agatagttgc cgatggcgtt gatcaacccc acattgtcca attccggaat
    16321 ctgcgtgctc tgctcgcacc acgcgctgat gccgcgcagg ctggccggca gcaaagcgtc
    16381 atcgccgagg aagattgtgc ggcccggata cgcctgcaaa aacgcgccgc tctggtaatc
    16441 cgccaacgac agcggcatcc actcaaagcc gttccacgca tgcagcgccg gcttgtccgg
    16501 cgagccttgg tagctcacca aaatcgtggg gaagcgccgc gccacatcaa aagccacttg
    16561 catcacgggg aagcgcgccg gtgccaccag cagcaccgcg tccgcctgcg ccgccgccgg
    16621 catcaccgcc acgctgctca gcgccaaggc caccactgcg atcatccaga aaattttctt
    16681 catcttgatc tccttccgca ttcctcgaaa tcgcccgcat tacaccagcc gtgtgggtaa
    16741 aatgcaagcc tttgccaaac gggtcaatgc tccgctgctt gggcgctggc tacgaccaac
    16801 tggccaaggc aaattccggc atcgttgggc ggaatttccc ggtgccgcca cacccggaag
    16861 ccggcccggg tcagcgcgtg cgtggctgtc gccagcagcc ggcgattctg aaaacaccct
    16921 ccggaaaggc acacatctgc cagcccctcg cgctgcgcca cggccaccat cattgccacc
    16981 agcgtttcgt ggaaccgcgc cgcggccgtg gctggcggct ccccccccgc cagcaaagcc
    17041 gtaatcatcg gcgcccaatc cagttccgtc ccacgccagc cgaacggata cggttcggcg
    17101 gcaaggtcgc cagccagtgc ttcgaggcgc atcgccgcct gcccctcgtg tgccatcacg
    17161 tggcaaatcc cgagcaacgc ggccaccgca tcgaacaagc gccccgcgct actggtgcgc
    17221 gccacattca cattctcccg caacatgcca tccagcaccg cgatctcctg cgcgctaaaa
    17281 cccagctccg gcagcggcgc acccatttcg cgcagtagcc ccagcgccac gcggcgcggc
    17341 tcccgcgccg cggcatcgcc accgatcagc ggaaacgccc gcagccacgc cgcgcggcga
    17401 tactccgcgc gggtgcacac cagaaactcg ccgccccaga tcgtgccatc cggccccagc
    17461 cccgtcccat cccacgcaat tcccagcgcc gggccggcca attgattttc cgcaaggcac
    17521 gccgcaatgt gcgcatgatg atgctgcacc gctgtcaacg gcacgcccca ctccgccgcg
    17581 ccgcgcgtgg cggcataatc cggatgcagg tccactgccg cgcgcgtaat gcgcagcccg
    17641 tgcagcgcgg tcagatcgcg cgcggtgcgc gcccagaggc gcgccgcgcc gtcgtgatcc
    17701 aaatcgccga ggtgcgggct catcactgcg ttgccaccca ccgccacagc cagcgcgcct
    17761 ttcatctgcg cgcccgcagc caccaagcca tccggcgcgc cggccaccgg cagcgaatac
    17821 ggcgccagcc cacggccacg gcgcagcacc acgggctgtt catccacaac tgccattacg
    17881 gagtcatcca tcggccgggc gatcggccgg ttgtgcagca ggaagaaatc agcaatatcc
    17941 cccaaccgct cgcgcgcttc ctcattatca atgcaaatgg gctcatcgct caagttgccg
    18001 ctcgtggcca ccaccgggaa gccaatttcg cgcagcagca aatgatgcag cggggtgtag
    18061 ggcagcatcg cgcctagcca gggcaaccgc ggggccacct cctcagcaat gcccggcgtg
    18121 gccgcgcgct tgcgcagcag cacaatcggt gcggcggccg attccagcca cgtctgctcc
    18181 gccgccgata cttcggccac tgcttggatg gccgccagcg aggggaacat cagcgcgaag
    18241 ggtttttcgt cgcgtcgttt tcgcgcacgc aaccgctgca cggccgccgc gtcgtgcgcg
    18301 gccaccatca gatggaagcc gcccaaccct ttgaccgcca caatccgacc cgcgcgcact
    18361 gctgccgccg ccgccagcag tgcagcatcg tgcgtggtct gcaccgtgcc atctgccgcc
    18421 gtccacgtca aatgcggacc acacactggg cacgcatttg gctgcgcatg aaaacggcgg
    18481 ttgcgcgggt cggcatattc cgccgcgcaa tctgcgcata gggtaaagcc gcgcatcgtt
    18541 gtgttggcgc ggtcgtaggg caacgcctca atcatgctga accgcggtcc gcagtgcgtg
    18601 cagttggcaa agggatagcg gaaccgccgg tcgcgcggat ccaggatttc gcgcaggcag
    18661 tcggggcatg tcgccaagtc cggcagaatg cctgccgcgc gcgcgccgtg cggcgtggcg
    18721 ctgctgcgga tttcaaaacc ggccggccaa tcgtccggcg cggagtgctc ggtcaccgcg
    18781 gtaattgccg ccgccgccgg caggcgcccg ggcaacgccg cgcggaacgc tgcaatggcc
    18841 gccgcgggcc ccgccaacgc cacctccgcg ccatcgccat cgttgcgcac ccagccgcgc
    18901 aggcccagct cgtgcgccag gcaaaacacc accggccgaa accccacccc ctgcacccgt
    18961 ccgcgcaacc gcaataaaat ggattcctct tccgccatgc tccgcgcaat ctacatccca
    19021 ccctcgccaa cgtcgagcgc ccgcacacgg aaacccgatt cccttggccg ccgggctgtg
    19081 ctacggtttt tgcatgagaa ggcgcaaacc atggcataag ggcaagtggc aaatctcccg
    19141 cgagcgcttc caaatcgcgg atgagactcc gcccctggaa gaccccggca tccagtccat
    19201 cggcgcgttc attccggcgg tgctgaacgg actaaagctg gacgaccacg cgcaggatgc
    19261 tgccattgtt gccgcctggc cagaactggt tggcccacag cttgccgcca acacccgccc
    19321 cgccaacttg gccaaccgcg tcctgaccat cttcgtcagt catcccggct ggctgatgga
    19381 attgcgcggc cccctgagcg cggaaatcct cacgcggctg caaaacaagt tcggcgccca
    19441 ctgcatcaag agcatccgcc tggccattga ccccaaaccg cgccgctgac caacccgtgc
    19501 cgacttgggc gttgcgcggc tccgcgcgaa tgcctacagt tttccacgcc atggaaaaaa
    19561 caaccggata ttttccacag tgtggaaaat tcgcgcgcca tttttccaca gtgtggaaaa
    19621 cttttttcca cagtgtggaa aacccggcgg ccgcacgcaa aaaaatcccg cgtcattttt
    19681 tccgggcgat cgccagcgcc ctgcttttgc tcgccgcggc cgctcccgcc agcgcggggt
    19741 ggatcgcgca aacctcgaat caggtcaccc tgaacctgac gctcttcaac ttgccggacc
    19801 cctcccgcac cgatccggcg acccgcgcgg agttggtcgt gcagcgcgaa tttttgcggt
    19861 tggcgcccga cctcctgcgc gcgcgccaag ccgccgaccc cgcgcgctat ggcgcgctgg
    19921 acttgcgcaa cctcgcggtg cagctccacc gcttttccgg cattcaggtg gagggcatcg
    19981 aaagcacgct gctggccatc gccggcaatg tggcgcccga tgtgctctac gtcaatttcc
    20041 gccaatctga aacctatatt tcgcaggggt ttttgtatcc cctcgaccgc ccaacggacg
    20101 gctacgccac cgcgttgccg cccgccgagt gggcgcgccg ggtgcatccg aaaatcgagc
    20161 ccgtcatccg ccgcccgggg ccggatggcg cggtgcacgt gtgggccatg cccagcggcc
    20221 cgccactcgg ccgcgtggtg ttgtaccgcc gcgatttgtt cgaggccgcc ggcctccccg
    20281 cgccgcagcc cgattggacg tgggacgatt tctaccgcgt ctgccggcag gtggccgacc
    20341 ccgcgcgcgg catctatgcg ctgggactct cgcgcggcaa acacgaatcc tatttgtggc
    20401 tgcccttcct ctggggcgcg ggcggcgagg cgctcgtgtt cgctgaacaa gaaaaccgct
    20461 ggcgcgcggt gttcgattcc cccgccgcgg ccaccgcgct cgatttttat gtgcgcctga
    20521 ccaccgagcc gtggaccgat gccgagggca aacgtcgccg cggctacgtc atcaaggata
    20581 ccgccgagtc caacctgaaa tggcggcgcg gccagttggc catgatgttt gcttatattg
    20641 atgacaagct gctcgccggg ctgaatccgg acctgaccgg catcgccccc ctgcccatcg
    20701 gccccgtcac gcgcggcacc gaaatcaatt cgcgcatgat gggtattttt gccggagtga
    20761 cgcatccgct cgtgcgcgat gccgcgtggg aatacatcaa tttccagtat tcgccggccg
    20821 ccgaagccat ccggattcag acgctggtgg ccggcggctt gggccgcttc ctgccgccgg
    20881 agcagctccg cgcgcagggc tatgagcaca tcgtcggcac catccctgcc gaatggcgcg
    20941 atagccacgc cattgccatc gccgccggcc gccccgagcc ttacggcaaa aatgccaacg
    21001 tcatttacga catcctcacc acgcccatcc gccaagccga agaactgtcg ctggccggcc
    21061 ggctggccac ggaccccgcc gcgcgccaag cgcaattgcg cgagttgctc gttcaggcgc
    21121 ggcgcaaggc cgatgccgac atgctcggcg tggtgccgcc cgccgagttg gccaagcggc
    21181 gcaccgccgc cagcgccttg ctgctggtga ttctcgccag cgtgctgctg gtgctgcgcc
    21241 acatggcccg cctcttttta cccgcccccg caacgcggcc cgctcccggc gctgcccccc
    21301 cccgcccgcg cgcaaccggc ggctggctgc gccgccactg gcgtgcgctg ctgctgctct
    21361 tccccgccgc ggccaccatt gcggtctggg gctatgtgcc tttggtgcgc ggcgcggcca
    21421 tggcgttttt tgattaccgg atttttggcg attccacctg ggtctttctc gacaactttg
    21481 ccgacctgct ctgggacccc gattggtggc gcgcgctgct gacttccgcg cgctacgccg
    21541 tgctcgtcat cacgctcacg tttatcccgc ccgtgctgct ggccattctt ttgcaagaag
    21601 tgccgcgcgg acgcatcgcc ttccgcctga ttttctatct gcccgcagcg ctgaccggac
    21661 tggtggtcat cctgctgtgg aaaacttttt atgaaccctc ggaagccggc ttgctcaacc
    21721 ggctgatcct gcccattccc gccggcggct ggttgctgct gagcgccggg gcgctgctgg
    21781 tggcgctgca cttggcacgc cggttattgc gacaccagtt gtggcgaccg gcgctcttca
    21841 ccgtggcggc gggtctcctg ctggcagccg cgccgctggg ccctctgcgc gaaatcatgc
    21901 gcaccgccac cacgggccat agctttgtca gcttgttcgc cacgctgccc gaacccgtgc
    21961 gctggctgga tgattcccgc accgcgttgc tggcctgcgt actgcccatg ctgtgggccg
    22021 gcatggggcc gggctgcctg atctatctcg ccgcgctcaa aggaatcccg gatgaactgt
    22081 acgaagccgc cgatatggac ggcgcatcgt tcaccgataa aatcctgttt gtggtcattc
    22141 ccactttgcg cccgctgctc atcatcaact ttgtcggggt gttcattgcc gcttggcagg
    22201 ccgaagccaa catcctggcc atgaccgccg gcggcgccaa cacggaagtc gccggcctgc
    22261 atattttcta caaggccttc atcttcctgc gcctgggccc cgccaccgcc gccgcctgga
    22321 tgcttggctg cctgctggtg ggcttcacgc tttatcaact ccgcatcctc tcccgcgtcg
    22381 aattccgcgc caaccgcgaa ggaacggcct agccatgccc atcctcgcca aaatcggccg
    22441 caaatcgtgg ggcacgcgcc tgcgcattgg cgcgatgtat tccgccctcg ccatcggtgc
    22501 cctggccatg ctctatccgc tgagcctgat gttggccggc tcgaccaaat ccatcgcgga
    22561 ccaacgcgac aacgtcctcc tgccacgaat gctggtctcc gacaccgctc tctggcaaaa
    22621 acatctcgag gcgctgttca acgaatcgct cgatgcgctc aacattgcgt tcgataccga
    22681 ttatccctcg tttgaatcca tccccctgcc cgcacccaac gccgcgcccg gcgcggatct
    22741 ggtgccgctg tggcgtgagt ttttggcgac gggggcactc acgccggaat ccatcgcgct
    22801 cggccactac tgggcgccac aggccggcgc atttcccgtg cagctccggg agttccgccg
    22861 ccatctgcgc gccaagtttg gcgacctgga cgcgctgaat accgcgctcg gcgccgactt
    22921 tgccgcttgg tacgcggttt tcatccagcc gccggcttac ctcttcccgc atactgttcc
    22981 ggccgccacg ccgctcgccg cggagtttga ttccttcaag ctcaatgcgc cgccgtggtg
    23041 ccagaccgtc ctttcgccag ccggattttt ccacaagctc tacctcaaac cgcgttactc
    23101 ggcggacatc gccgcgtaca atgccgcaca tggcacggct tacgccgcgt atgctgatat
    23161 tcccctgccg cgcacctgcc ccgccgatgc cccgccgctg gtgcaggaag attggcgcga
    23221 cttcacccaa tactcgctcg cgccgctctg gctcgtggac ggtgccctcg atacgccgga
    23281 aacccgttgg cacgactggt tggcccgcgc ccacccttcg gtcatcgccc ccggctcctc
    23341 cgtccccccc atgcctcagt tggcgcacca tgtccaacta tttcgcgaac accgggccgc
    23401 catccgccat gaactgctgt ggcgcaacta ccgcaccgtg ctcgactata tcctcttcca
    23461 tggtcgcggc atcctgaaca ccacgatcta ctgcgcgctg gccgtgctgc tcgccctact
    23521 ggtcaacccg ctcgccgcct acgccctcag ccgcttcaag ccggccgtcg ggccgcaaat
    23581 cctgctgttc ctgatgctga ccatggcctt cccgcacatg gtcacgcaaa tccccgtgtt
    23641 tgtccttctg cgcgagttgg gcttgctcaa caccttcgcc gcgctgctcc tgcccggcat
    23701 ggccagcggc tactccat
//
LOCUS       DAOPNP010000028        23156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2776_length_23156_cov_7.464958, whole genome shotgun sequence.
ACCESSION   DAOPNP010000028 DAOPNP010000000
VERSION     DAOPNP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23156
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2776_length_23156_cov_7.464958"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..685)
                     /locus_tag="PKI64_03955"
     CDS             complement(143..685)
                     /locus_tag="PKI64_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOE00198.1"
                     /translation="MSHFLLSVIHALVRGLDRLERLILTVLVTGMVILAAVQILLRNI
                     WHTGFNWAEPLLGMALLWLTMLGALAATGQSRHISIDLAAAFLPKRGQAILRRLTGLF
                     AVVVCTMLAWAAGRYVGFQSEMETNILLGWPLWKYYLVVPVVLWLMAWRFLVHSLLPA
                     AWLNSEDTPAESAGQGRGGA"
     gene            complement(682..1677)
                     /gene="dctP"
                     /locus_tag="PKI64_03960"
     CDS             complement(682..1677)
                     /gene="dctP"
                     /locus_tag="PKI64_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOE00199.1"
                     /translation="MRTLRWMWIVGALGLGWAATGEAQTTLKIATMAPDGSIWMQALA
                     DAKTEVETATGGAVKLKIYPGGIMGSEKDVLFKIKTGQLQGGGFMGYAIGKICPDASA
                     LMFPMVFHSYAEVDQTLPKILPYLDDHARQNGFESMGWTEVGFSYAYSTKPIANLEEF
                     RATKVWGLDSPMLTELLVAAGVSFIPANITDVLTSLQTGLLETVFGPPAAAIAVQWHT
                     KVRYYNEMQLTYAIGGVFISSASWAAVPAQHRDTVKSIIAKHCQLLTPKVRKSDIEAL
                     EFMRKQGVQALAETPAARAAFDNVAETALGKIKGTVFSAEVWERLQNILRDIRGS"
     gene            complement(1692..2573)
                     /locus_tag="PKI64_03965"
     CDS             complement(1692..2573)
                     /locus_tag="PKI64_03965"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOE00200.1"
                     /translation="MKLLQPWMLPVLLTAGMLLNSGCVLVRKGAARMISPVATQLSDG
                     FMRENDVDLVRDGAPAFLLMLDALAEAHPDNPAVLLAAAEAQLAYAVGFVGQEQKERA
                     KTMLLKSKTFGLRALSKKNKHFAAAVDGSYDDFRQALTRFKQPDAEALLSTATAWVMW
                     IIANSDSPAALGDMPKVMELMQRVHVLDPDIRQGGVDQFYGIYYTVLPLGAGRDLDKA
                     REHFEHSIQVAGPDYLLNQVTFAEFYARYALDQELFERTLRNVLAAEPGVPEFTLMNA
                     VAKMRAQTLLSQMDDLF"
     gene            complement(2625..3413)
                     /locus_tag="PKI64_03970"
     CDS             complement(2625..3413)
                     /locus_tag="PKI64_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOE00201.1"
                     /translation="MLTTKRFDYVPFDHVHLHPSVANHRPLNEAKVAHYAEDILRNGL
                     LEPIVVWERNPGEYYLVGGFHRHNAIRRIRAQNPGYFDRVDVRVVQGELDEIQALNLK
                     LNADRLDARITDYFDAIIFMANANWDKNRIARFLDKNVAWIEDILRYAPSMDSRIRRL
                     LEQGEISWAKAKAICKAVLTAPPGQERATVDAALAALQAPAAEKPARALTFRKATKRL
                     AASKTTYQVTGADVAALLKVVRVQSASAEDVARVRAAFPDLLED"
     gene            3573..3962
                     /locus_tag="PKI64_03975"
     CDS             3573..3962
                     /locus_tag="PKI64_03975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019262188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE00202.1"
                     /translation="MFTKTITPRFGDADGLRHINNTRLPIWFELGRDPFFRFFNPEML
                     FEKWNLIMAHIDVDFVAPLLLGAEIEIRTYVAKVGNTSFTSYQEAWQNGQLCAKGNAV
                     VVHYDFKNRKPLPIPDDIRARLLEHRP"
     gene            3959..4276
                     /locus_tag="PKI64_03980"
     CDS             3959..4276
                     /locus_tag="PKI64_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00203.1"
                     /translation="MMGRAGMTTADLAILVAVVALIAAIGIPAARGNRQRSHAARCAM
                     NLDILAAAAQRFVADHGQAPGAVKELVPAYLETLPHCPAGGTYALGADGQPPTCTIPG
                     HHF"
     gene            4300..5019
                     /gene="pyrH"
                     /locus_tag="PKI64_03985"
     CDS             4300..5019
                     /gene="pyrH"
                     /locus_tag="PKI64_03985"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOE00204.1"
                     /translation="MKKAKYRRILLKLSGEVLRDNATGECLADSVLADMAKQIKAIRQ
                     LGVQVGVVIGGGNIFRGLKGQDRGFGRAHGDNMGMLATMINSLALQSALEAAGVPAKT
                     LAATAMPALCELFTFRRALELLADGCVVIMGGGTGNPFFTTDSAAALRAAEINAEVLL
                     KATKVDGIYDSDPVKNPQAVKFDRLTYSEALARRLKVMDAAAFSLCMDNQIPILVFDF
                     FKRGELLRVVTGQKAGTLVTA"
     gene            5044..5610
                     /gene="frr"
                     /locus_tag="PKI64_03990"
     CDS             5044..5610
                     /gene="frr"
                     /locus_tag="PKI64_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOE00205.1"
                     /translation="MYESTDDVLLETDDKMEKAIAHLQQGLSGLRTGKATPSLVDYLM
                     VECYGTQTRLNQMSLISTPEPRLIVIKPYDPSSLPAIEKAILAANIGVTPLNDGRIIR
                     VPIPELSEERRRDLTKVANRMGEEARVAIRAVRREANDAVKALEKAAKISEDDRDRTL
                     NEVQKYTDNSVKKVDDLVAAKEKDIMAV"
     gene            5825..6802
                     /locus_tag="PKI64_03995"
     CDS             5825..6802
                     /locus_tag="PKI64_03995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE00206.1"
                     /translation="MVPPTPQTNLLNPDAPGVLQPTGSGRLESAKFGSTRTGVRNGRA
                     YPAFHEGVDIAATMRDRKGVATDPVYAAAAGRVAFINSAAGNSSYGKYVVIEHPDPSL
                     GSIKLRDGSSVPATVYTLYAHLADIRFLVRPGHAVEAGDIIGTMGATSNTQPPIPRDR
                     SHLHWEIGVMLNARFETMAREQKLVPDFGNYNGGNLFGLDALDFYAAHARDPELTMAG
                     YLAQLRPACEVVLRGKYPDYFRRYPALWRGAAHDGGPIHLAMSESGAVISGRNATATE
                     VALLGNQRQAVSKVYEDVLGRNGRAYVAQTGGKWQFTEKGRQWANILFY"
     gene            6898..7764
                     /locus_tag="PKI64_04000"
     CDS             6898..7764
                     /locus_tag="PKI64_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00207.1"
                     /translation="MAKLLKPLVVIVLLLSIAALCIQGFVLFPKRTLIKDRTQKLEGG
                     VDRLVSTLKSSLPEETQRAVPFQRSNLLIGTTEDLPRLDGEINKAVQVANAVLQGWND
                     TKQDLENTRQDLENTRAELANTREQLEDARTQIVQLNDTIRAKDQELQEKSDLIASLE
                     EEKAALTSQVADLENQVASLQDQIAELEEDKALLETQLEKCDKELHKEITMKPGTTGT
                     IVYANAEWNFVVIDIGSNDGVQPTAEMIIHRGDNMIGKIKISAVRDNVSIAEVMSEYQ
                     TANVKEGDHVLF"
     gene            7751..7873
                     /locus_tag="PKI64_04005"
     CDS             7751..7873
                     /locus_tag="PKI64_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00208.1"
                     /translation="MFSSNGLSAMTLIAFLLLVAVIGLQVAEIHHYSAIPSLWP"
     gene            7870..8034
                     /locus_tag="PKI64_04010"
     CDS             7870..8034
                     /locus_tag="PKI64_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00209.1"
                     /translation="MRPSGLCLTLLLLALLVWSGAGCATPHDESDLPWNMPQSWESAP
                     SLPFGAAGGY"
     gene            complement(8054..9808)
                     /locus_tag="PKI64_04015"
     CDS             complement(8054..9808)
                     /locus_tag="PKI64_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE00210.1"
                     /translation="MSLQPISRRESRQPLVQAALFAAALVVSLALHAVLLIEFPAMSL
                     GRPADWPDKLKPHPLVLGEVRALPDLPAYTQPPKFRPENPENFATIEPLQKSLLEELQ
                     IAAEGVEIEAPPISEAIPPQAAPPAERSALDFRQDILQIEQRVAADELAALPRFTLPA
                     VERVETAPDITLPATAEAIAAAAAASQGRGDSSVQALAVLSQATPSASDMIAAANAGA
                     GTEADRLHEQGSVLDEKAADITDIQPIEQLLEVAVSTFKASDEDALYFELSIARAGVA
                     ALPVMPKDILLVQDCSESMTRTKLDFFKDGIESYLGTLTTADRVNVMRYSDSPTLCFP
                     DWQPVTSESLAAAMRFTDNMRARGQTDLFLPLQEVLKLPKQSGRPMIVVLMTDGRPTL
                     GTVDSSDIIARFSRTNKGEISVFTIGAGDRVNTFLLDLLGHNNRGDSWFRPLREDIPA
                     TVKRAARELSRPVLADLTYRFASDSGAEVYPATLTHLYLDRPLRLVGRCPANQKTAVL
                     QILGEAAGQQRDMVFALDLADATEGGEGLRREWVAQKIYKLINDHIATGRADTIREIR
                     ELSIRHNVPLPYGADFPL"
     gene            complement(9821..11656)
                     /gene="rho"
                     /locus_tag="PKI64_04020"
     CDS             complement(9821..11656)
                     /gene="rho"
                     /locus_tag="PKI64_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOE00211.1"
                     /translation="MATNPKQPRQPRAAGGARRGRPPRKKAGEDDQKRPAMPLPSSLL
                     ETTIADGEEFHPGDAPAAQPEATAPVAGTEPAAPEMAPPPAPFVPYESRESEPPAPPA
                     PDAAVSSGQQNGNGGHPAYPQHHRPHYQQGGGGGRHDRYFDRNRRHGGQPHHDNRPPR
                     PPLTPEEQAAREAQIAAQRAAAEAAAAERAKMARVLKLNELQQLTVPQLKELADSYQL
                     VDLGALKKHELIFEILKANARCNGTMFVRGVLEILPDNFGFLRSPNNDYLPCPDDIYV
                     SPSQVRRFALRTGDLVEGEIRSPKDKERFFALQKVSSINEGPPEGQRGKIPFENLTPL
                     FPNKRLVLETKSKNNINMRVMDLLTPIGKGQRGLIVAPPRTGKTVLMQMIANSISENN
                     PEVKLIILLIDERPEEVTDMERNTQAEVIASTFDEPPERHVQIAEIVIEMAKRQVESG
                     KHVVILLDSITRLARAYNTLQPHSGKILSGGVDANALHKPKRFFGAARNIENGGSLTI
                     IATALVDTGSRMDEVIFEEFKGTGNMEICLDRFLVDKRIFPAINIEKSGTRKEELLMH
                     PDELSRVWVLRKALNGVPAVEAMELLIGRLKKTKTNLEFLMTLQG"
     gene            complement(11659..12282)
                     /gene="coaE"
                     /locus_tag="PKI64_04025"
     CDS             complement(11659..12282)
                     /gene="coaE"
                     /locus_tag="PKI64_04025"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOE00212.1"
                     /translation="MVMKPQAPASLCIALTGGIACGKSTVAEIWRREGACILDTDELA
                     HQLIEPGGEYVESVLREFGAHLRTPQGGVDRQQLGAQVFADPAARARLNALLHPPIKA
                     RVRAWCESVRQQHRKGVAVIPLLFEAGMDDEWDAVVCVAATLETMRARLTARGLKPAE
                     ADARIASQMPIAEKKARATHIIENDGTLAELTAKSLALWKNLVEKGN"
     gene            complement(12276..14393)
                     /locus_tag="PKI64_04030"
     CDS             complement(12276..14393)
                     /locus_tag="PKI64_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00213.1"
                     /translation="MLRRPWLKIVLLGVLLLGLYAGGVATRRHVLNAQRELTSDGSIP
                     FTLESALAFRRIQMVYRDGALPAVDRGIQYPQGVVSREVDTIGVEKLYVRAAQLWPGA
                     FPLAEKIRWLHLLWFCLAIPGMYFWVRWLGGGGRGGLWAAAFYAVAIGAVARSTGQEL
                     SHENNALPLLIWSMAVLAWSRQALHWRSKMAGGWLAALLVGAALCAWDLIQFYLLLFL
                     AWHLVEAIRGRLGKTELWTVMIPLMAILLAAGLRNPYLRSHGFLFSPAMALGWGMLLA
                     GAPIAQRQHKATRVILGLLPLLLASLWAGLYLPAYSHFGSLLWAKLRFFNIRPTDPAL
                     LTFEQRILWAPALNSTSWLLLWQWFPALLVLTLAAIWGLLRIAFRTRCDQAGFFPLLF
                     YFLVSFFTFILFFRFHVWVAIFGCGLVGLWAGQISCGENPRWRGVMASVLVCGLLLEA
                     AQPWLGPLVREKKAFEAPEAKKWTGSLFWGRPNVYAAETDALMKHLRKYDAPEPVLAN
                     FGISAAIATYGGCPVVLHPKFESAAIRRKVREYGEALFKGDEAEFRDWMEAQGATIYV
                     HSMGEFAAIEPGLQMRYMVDALDPATNVAARLFEQRPEELQFFAPEFANRKYRVYRLK
                     QSPVAARLAETLAERARIALENGQLAPAELQAAHALRMDADNTEAQAVLRHADALRQS
                     GFRAEEEDWFEEEADPLAPAKPW"
     gene            complement(14393..15319)
                     /locus_tag="PKI64_04035"
     CDS             complement(14393..15319)
                     /locus_tag="PKI64_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOE00214.1"
                     /translation="MSSSSLIIDGTAVAYRAFYALQDLSTRAGQPTNALFGFIRMLQQ
                     LAAKWRPDKLVVVFDGGSPARRLEKLPEYKAQRAPMPDALRSQLPLLNEYLAAAGIPL
                     VLIKGQEADDVMATLAVRAAKQGGTVRLATSDKDLMQLVGENIRIVPPTKAEEELDAA
                     GVEAKTGVRPEQIVDWLALVGDSADNIPGVHGIGPKTAAKLLRQYGSLAQCAAQLDNI
                     EPGGVREKLRAELDVAKLNVELMTLDTAVPGVPEWPDIAPPAPDRQQLLDFFNKFEMH
                     RFAAELSTQPAVPPKTTLKPPATPGNTQLELF"
     gene            complement(15480..18509)
                     /locus_tag="PKI64_04040"
     CDS             complement(15480..18509)
                     /locus_tag="PKI64_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00215.1"
                     /translation="MKWCMVAAMTVALLFGSVALAQDDGFDLDALLGDADTATASVAD
                     ATADVAVAEAPAEEAEAVADEFVSETPVAEVADEFAAPAEEPVAEADPFADQAAEPAV
                     EAEEVAADDLDSSADLFADESAPLAEEELPADVVEAPAAAADDLLGDLFGEAPAAEPV
                     AEPADDFAGFDEPAVAATDDFAAPDYGDDDDATMAEFTPAGTVVTPSRAKATKPAEKL
                     DPKELKKLAKETAQQEELRRQAAEADALKASDAGFAALSKGEYAKAESALNSALINLP
                     DRPQNAAVRDQIMKGLAEAQYLRAHALVQKKERPEEARRLVDSALKNDPAHRGALALS
                     KKLDKIEAEIARPKLPAEQPATLQKQADIGKLYDEARQWYMLKDYDRAKAAFEQILKL
                     DPYHKSAMRYLRKLEEDKFKLATYERHARVQEMMTDVRRSWSPPTRLEVAQLDQAGEN
                     AGVNTKPASQRLQEKMKEIIIPAVEFRQANINDVVSFLVDASVAADPEKEGVNIILNL
                     GSGGGGAAPAAAAPAPAADEWGFGASDFDMAMPASSGAPGVRDVTLNLRRISMLDAIK
                     YITEVAGLKYRVEDAAVIITSVDTPVGNIITRMYPVQPSFMDVVFERQSASDDRQSSE
                     FVSMGDRSTATKSDIKEFFEKTGVKFPAGASVTYNATISQLIVANTADNLETFEQILQ
                     RLNVVPNQVEIEARFIEVNQDDLEELGLQWILNDNYEFATKNDGSGQRMQVNANKNGL
                     TQGNRFFNYSIDTYLTSPASPITRAAGQTPLGGILSASSVLTNPEVTVVLQALSQHGG
                     TDLLSAPRVTTRSGVNAQIQVVREIIYPTEFDLTQPTVDTDGHVTMAPIVTPGTFQTR
                     ETGVILNVTPTVGPDGYTIDLVMAPEVAELVDWIQYGSSITISGSDLYFFNMPMPVFT
                     SRNVTTSIVIWDGQTVVMGGLIREEMTTIKDKVPILGDIPVLGWLFRSEGHYSQKKNL
                     LIFVTARLVDPAGRPVHTESNMVMPGAGLDAGTASVE"
     gene            complement(18598..19665)
                     /locus_tag="PKI64_04045"
     CDS             complement(18598..19665)
                     /locus_tag="PKI64_04045"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00216.1"
                     /translation="MSQALRGKNDWLRDHYEKIIFLVALVALFISCILVSQWILADKD
                     HDKPNLARIGWKGEPVKIKDTLPFEEALDSARKEATVALTVPERSTISEVRVACIKCG
                     KPIPYSALSCPFCLAEQPPIINVDTVDTDGDGIPDKVELALGLNPQDPADAAGDLDND
                     GFTNLEEYLAKTDPRNPENFPDPIVKLRVAAIRPVPFYLRFVSTSLFGDGSTRFQLNL
                     QSLERTYFVKLEDDVLGYQVTKYNPTAPEGESIILVRKADKHAVRLVKGRPVTEQELA
                     ILFVNLIDRQPLRPPKRLNDVFAFRNKEYKVIDIQPASVVIQETTTGDKVTVPVVTSA
                     EKDALNPSAAAANLLTAAPGQ"
     gene            complement(19662..20669)
                     /locus_tag="PKI64_04050"
     CDS             complement(19662..20669)
                     /locus_tag="PKI64_04050"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOE00217.1"
                     /translation="MNRKYMPLVIGGVLVLVVVLGLGYLLFSAKSNYADRRTNLEREQ
                     LKLRNLTNRNPFPADTNVVILSKQKAVFETYLGDLLGVLKQGQTPVGDLTRDRFRQLA
                     EAKLRELTDEARKRNVVLPANFAFGFQRYAEGNLPPDDEMERLVDQFKSIAILCDVLF
                     KAGISELVAVERTVFERDAQPAPVEESYGRRTRGREEAEVQAPKLDLQVDPLGLYTKE
                     HYQFTYKAKDEAQWEVLNRLAKGMPFTVVTRVEITNLAKPVVTPPQTKPEEHAAAPVS
                     TAGWAAPGSLQPPPKPEIEILPRDLRVTAGRDLPTIVLDVDMYRFLEPAADPAQGEEK
                     S"
     gene            complement(20666..22351)
                     /gene="pilM"
                     /locus_tag="PKI64_04055"
     CDS             complement(20666..22351)
                     /gene="pilM"
                     /locus_tag="PKI64_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOE00218.1"
                     /translation="MAKKPILTLDIGSHSLKLAEFMDVKGGLEMTRYAVSDLGVDSQS
                     DADRSQYIITTINDLIRESGCKPGPVQVAISGHSVFSRFVKLPPVEPEKVYQIIQYEA
                     QQNVPFPMNEVVWDYQLISGATGDVDVMLAAIKADIINELVDCVRFTGLVPDLVDMAP
                     IALYNAVRYNYDNLPPCTLVVDIGARSTDLVFIEAGRIFSRSVPVAGNQITQAIMNEF
                     SISFDDAEAMKKAHAYVAFGGAYEGPQSETVDKVSKSVRSMMTRLHQELNRSINFYRS
                     QQGGQQPALLLLTGGSSVIPYTDTFLKEKLNIATDYFNPFNNVAVAEQIPAEQIGAHA
                     CEMGPLVGLALRQLHSCPIEINLLPPKAEEDKKFKRKLPIFAMAAGVLLLTLAVWVAF
                     YTRMANVADRTSELIAKRVGELSRVENQLRGVESSVGEVEKKIAALTTLESRRSTWRS
                     VLGAISTPLPPGMWLTRVHPLATLPGENQPATQNPQAADSGLSNQVAGIEIEGMAYQD
                     KIKSEAIGQYRDQLRESPWFSDKTEIIWQPPARGDSATLEFKILAVLKEPQVL"
     gene            complement(22372..22980)
                     /locus_tag="PKI64_04060"
     CDS             complement(22372..22980)
                     /locus_tag="PKI64_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00219.1"
                     /translation="MMQTLRVRTVCGVVLFVALWLVAGAAWAQMANALPAGSDILRIR
                     KMGPVANTKTPVFKTQVQSTSSAQQPDWWRHLVEYETAPDWLDELEFTYYVYMRDQSN
                     KGAEVMFRATVTYVNVPKGRHYSDIFLHPRTLARLGKVEQVAVVVKARGAVVATESTA
                     QTPNWWDRFSPVDGVLLNRSQTPFAYLDYDQFEAIKPVAPAR"
BASE COUNT         4726 a         6989 c         6680 g         4761 t
ORIGIN      
        1 atgggcatgc tggtaagccg ggtcaattcg ccaatcacaa tggaaaaatc catgccctcg
       61 gcatgatagg cggtcatggc cagcgcgcca aaaatggcga acatgggcac gcccagcagg
      121 gcgcagaaga gcacaaacca aatcatgctc cccccctccc ctgccccgcg gattccgcgg
      181 gtgtgtcttc gctgttcaac caggcggccg gcagcaggct atgcaccaaa aagcgccaag
      241 ccatcagcca cagcaccact ggaaccacga gataatattt ccagagcggc cacccgagca
      301 ggatgttagt ttccatttcg ctttggaagc cgacatagcg cccggccgcc caggccagca
      361 ttgtgcacac cacgacggca aaaagaccgg tgagccgccg gagaatggct tggccgcgct
      421 tgggcaaaaa cgcggcggcc agatcaatgg aaatgtgccg actctggccg gtggcggcca
      481 acgcgcccag catggtcagc cacaggagcg ccatgcccag caggggctcc gcccagttga
      541 atccggtatg ccaaatattg cgcagaagaa tctgcaccgc cgccagaatg accatgccgg
      601 tgaccagcac cgtcaggatc aagcgctcca gccgatccag cccgcggacc agcgcatgaa
      661 tgacgctaag caggaaatga ctcatgagcc gcggatatcg cgcaaaatat tttgtagccg
      721 ttcccaaacc tcggcggaaa atactgttcc cttgattttt cccaaggcgg tttcagccac
      781 gttatcgaag gcggcgcgcg ccgccggcgt ctcagccagt gcttgcacac cctgcttgcg
      841 cataaactcg agcgcctcga tatcgctttt gcgcactttc ggggtcaaca actggcagtg
      901 tttggcgata atggatttga cggtgtcgcg atgttgggcc ggcacagcag cccaactggc
      961 cgacgagata aatacgccgc cgatggcgta agtcagttgc atttcgttgt aatagcgcac
     1021 cttggtatgc cactgcacgg caatggctgc cgccggcggg ccaaagacag tttccagcaa
     1081 gccggtttgg agggaggtca gcacatcggt gatattggct ggaatgaacg agacccccgc
     1141 cgccaccagc agctccgtca gcatgggtga atccaagccc cagaccttgg tggcgcggaa
     1201 ctcctccaaa ttggcaatgg gtttggtgct gtaagcatag ctgaagccca cttccgtcca
     1261 gcccatgctt tcaaagccgt tttgccgagc gtggtcatcc agataaggca ggattttcgg
     1321 aagcgtttga tccacctccg cataactatg gaacaccatg ggaaacatta gcgcgctggc
     1381 atccgggcaa attttgccaa tggcatagcc catgaaaccg ccgccctgca gttggccggt
     1441 tttgattttg aacagcacat ctttttcgga gcccataatg ccgccgggat aaatcttcag
     1501 cttcacggcg ccgccggtgg cggtttccac ttccgttttg gcatcggcca gcgcctgcat
     1561 ccagatgctg ccatccggcg ccatggtggc aattttcagc gtggtttgcg cttcaccggt
     1621 agccgcccag cccagcccca acgcccccac aatccacatc caacgcaaag ttctcatgct
     1681 gcttccttat tttagaagag gtcgtccatc tggctcaaca gggtttgggc gcgcatcttg
     1741 gccacggcat tcatcagggt aaactccggc acgccgggtt cagccgccag cacattgcgc
     1801 agcgtgcgct caaacagttc ctgatcaagc gcgtagcggg cataaaactc ggcaaaggtc
     1861 acctggttga ggaggtaatc gggaccggcc acctggatgc tgtgttcaaa gtgctcgcgc
     1921 gccttatcga ggtcgcgccc tgcgcccagt ggcaacacgg tgtaataaat gccataaaac
     1981 tggtccaccc caccctgccg gatatctgga tccaacacgt gcacccgctg catcagttcc
     2041 atgaccttgg gcatgtcgcc caaagcggcc ggcgaatcgg agttggcgat gatccacatg
     2101 acccacgcag tggcggtgct caacagcgcc tcggcatccg gctgcttgaa gcgggtcagc
     2161 gcttgccgaa aatcgtcata ggagccatcc accgccgccg cgaaatgctt gttctttttg
     2221 ctgagcgccc gcaggccgaa cgtcttggat ttgagcagca tggtcttggc gcgttctttt
     2281 tgctcctgcc ccacgaagcc gaccgcgtag gccaactggg cttcggccgc cgccaacaaa
     2341 acggcgggat tatccggatg ggcctcggcg agggcgtcca gcatgagcaa aaatgccggg
     2401 gcgccatcac gcacaaggtc cacgtcgttt tcgcgcatga agccatcgga gagttgcgtg
     2461 gccaccggcg agatcatccg ggcggccccc ttgcggacca gcacacagcc actattcaac
     2521 aacattccgg ccgtcagcag caccggcaac atccatggtt gaagtagttt catgacttat
     2581 ccttctacgg tccccgaccc acgggaacaa gctcaaacgg caggtcaatc ctccagcaaa
     2641 tcgggaaatg ccgcacggac ccgcgcgaca tcttccgccg acgcactttg cacgcgcacc
     2701 accttgagca acgcagccac atctgcgccg gtgacctgat acgtggtttt gcttgctgcc
     2761 aaccgcttgg ttgctttgcg gaaagtcagc gcacgcgctg gtttttccgc ggcgggcgct
     2821 tgtagcgccg ccaacgcggc atccaccgtc gcgcgttctt ggccgggggg ggcggtcagc
     2881 accgccttgc agatggcctt ggccttggcc caactgatct cgccctgctc cagcaggcgg
     2941 cggatacgcg aatccatgct gggcgcatag cgcaaaatgt cctcgatcca cgccacattt
     3001 ttgtccaaaa agcgtgcgat gcggttttta tcccagttgg cgttggccat gaaaatgatg
     3061 gcgtcaaaat agtccgtgat gcgcgcatca agccggtcgg cattcagttt gaggttcagc
     3121 gcctggattt cgtccagttc gccctgcacc acgcgcacgt ccacgcggtc gaaatagccg
     3181 ggattctggg cgcgaatacg gcggatggca ttatgccggt ggaagccacc caccagataa
     3241 tattcgccgg ggttgcgttc ccaaaccacg atgggttcca agaggccgtt gcgcagaata
     3301 tcctcggcat aatgagccac cttggcctcg ttcaacggcc ggtgattggc caccgaagga
     3361 tgcaaatgca cgtggtcgaa aggcacataa tcaaatcgtt tagtcgtcag catggggttc
     3421 cttgtttgag cccgcacact ctacgcattg gtttccccgc ggtgaagtat ttttttctct
     3481 ccgcgagcgc cggaaagcgt gtgctttcat ctttactcca aggggcattc tccgtttaat
     3541 ccaccctgtc tgtttgaacc catttttttg acatgttcac caaaaccata accccgcgct
     3601 ttggcgatgc cgatggcttg cggcacatca acaacacccg cctgcccatc tggtttgagc
     3661 tgggacgcga tccgtttttt cgcttcttca atccggagat gctgtttgaa aagtggaatc
     3721 tgatcatggc gcatattgat gtggattttg ttgcgccgct gttgctgggc gcggaaattg
     3781 aaatccgcac ctacgtggcc aaggtcggca acacctcgtt cacttcctat caggaagcct
     3841 ggcaaaacgg acaactgtgc gccaaaggca acgcggtggt ggtgcactat gatttcaaga
     3901 accgcaagcc gctgcccatc cccgatgaca tccgcgcccg gctgctggag catcggccat
     3961 gatggggcgc gcgggcatga ccacggcgga cctcgcgatt ctggtggcgg tggtggcgtt
     4021 gattgccgcc attggcattc cggcggcgcg cggtaaccgg caacgctctc atgcggctcg
     4081 ctgcgccatg aacttggata ttctcgccgc ggccgcccag cggtttgttg ccgatcacgg
     4141 ccaagcgccc ggcgcggtca aggagcttgt gcccgcctac ttggaaaccc tgccgcactg
     4201 ccccgccggc ggcacctatg cgttgggggc cgatggccag ccgcccactt gcaccatccc
     4261 gggccaccat ttctaatgac gacacgacag gagtagcaca tgaaaaaagc caaatatcgc
     4321 cgcatccttt tgaagcttag tggagaggtc ctgcgcgaca acgccacggg cgaatgcctt
     4381 gccgacagcg tgctggccga catggccaag cagatcaaag ccatccgcca actgggcgtg
     4441 caggtggggg tggtgattgg cggcggcaat attttccgcg gcctgaaagg ccaggaccgc
     4501 ggcttcggcc gcgcccatgg cgacaacatg ggcatgctgg ccaccatgat caacagcttg
     4561 gcgttacagt cggcgctgga agccgccggg gtgccggcca agacattggc cgcaacggcc
     4621 atgccggccc tgtgtgagtt gttcacgttc cgccgcgcgc tggaactgct ggccgacggg
     4681 tgcgtggtga tcatgggcgg cggcaccggc aatccgtttt tcaccaccga cagcgcggcc
     4741 gcgctgcgcg ccgcggaaat caacgccgaa gtcctgctca aggccaccaa ggtggatggc
     4801 atctatgaca gcgacccggt gaaaaatccg caagccgtca aattcgaccg tctgacctac
     4861 tcggaggcat tggcgcgtcg gctgaaggtc atggatgccg ccgccttttc gctttgcatg
     4921 gacaaccaaa tccctatact ggtttttgac tttttcaaac gcggtgaatt gctgcgcgtg
     4981 gtcaccggcc agaaagccgg tacgcttgtc accgcctaac cctctgatcc gaaaggaaag
     5041 gccatgtacg aatccacaga tgatgtcctg cttgaaaccg acgacaaaat ggaaaaggcc
     5101 atcgcgcatt tgcagcaggg gctttccggc ctgcgcaccg gcaaggctac gccgagtctg
     5161 gtggattacc taatggtgga atgctacggc actcagaccc gtctcaacca gatgtcgttg
     5221 atcagcacgc ccgagccgcg tctgatcgtc atcaagccct atgacccttc ctcgctgccg
     5281 gcgattgaaa aagccattct ggcggccaat atcggcgtca ccccactcaa tgatggccgg
     5341 atcatccgtg tgccgattcc cgaattgagc gaagaacgcc gccgggattt gaccaaggtg
     5401 gccaaccgca tgggcgaaga agcccgcgtg gccatccgcg cggtgcgccg cgaggccaac
     5461 gatgccgtca aggccctcga aaaagcagcc aagatttcag aagacgatcg cgaccgcacc
     5521 ctcaacgaag tccaaaaata caccgacaat tctgtcaaaa aggtggacga cttggtggcg
     5581 gccaaagaaa aagacatcat ggccgtttga ccggccatgg gaccgcgcac gcgcaagtcg
     5641 cccctgcgcc atttgctttg gatcattccg ctggtggtgc tggatttttt tattttccgc
     5701 tggctggccc tgcggccttc cggttgccag atgccatcgg ccccaccccc gccggcggtg
     5761 gaggatgtga cgcccccccc ccccccccgc cccccccccc ccccccccgc ccgtttggca
     5821 ggccatggtg ccgccgactc cccaaaccaa tttgctaaac cccgacgccc ccggcgtgct
     5881 gcaacccacc ggatcagggc ggttggagtc ggccaaattt ggctccaccc gcaccggcgt
     5941 acgcaacggc cgcgcctacc cggccttcca tgaaggcgtg gatattgccg ctacgatgcg
     6001 cgaccgcaag ggcgtagcca cggacccagt ttatgcggca gcggctggcc gtgtggcctt
     6061 catcaattct gccgccggca attcgtccta cggcaaatat gtggtgattg agcatccgga
     6121 cccttcgctg ggctctatca agctgcgcga tggctcatcg gtgcctgcga ccgtgtacac
     6181 gctttatgcc cacctggcgg acattcgttt tcttgtgcgg ccgggccatg ccgtggaggc
     6241 tggcgatatc attggcacca tgggcgccac cagcaacacc cagccgccca ttccgcgcga
     6301 ccgcagccat ctccattggg aaatcggcgt gatgctgaac gcgcgctttg aaaccatggc
     6361 ccgcgaacaa aagctggttc ccgacttcgg caactataat ggcggtaatt tattcgggct
     6421 ggatgcgttg gatttttatg ccgcgcacgc gcgtgatcct gaactgacca tggcgggcta
     6481 tttggcccaa ttgcgcccgg cgtgcgaggt cgtgttgcgc ggcaaatatc cggattattt
     6541 ccggcgctat cccgcgctat ggcgcggcgc agcccacgac ggcgggccaa tccatctggc
     6601 catgtcggaa tccggcgcgg tgatctccgg tcgtaacgcc accgccacgg aagtcgcgct
     6661 gctcggaaac cagcggcagg cggtcagcaa agtatatgaa gatgttctcg gccgcaacgg
     6721 ccgcgcgtat gtggcgcaaa ccggcggcaa atggcaattc accgaaaaag gccgccaatg
     6781 ggccaatatt ttgttttatt aagatgaaac tgagacctgc cgacgaagtg ccccttttga
     6841 catttgacaa tgcggcttct ttgtcacaga atgccgacac aatccttgga ggaatttatg
     6901 gctaaattgt tgaagccgct ggttgttatc gttttgctgt tgagcattgc cgctttgtgc
     6961 attcagggct ttgtgctttt cccgaagcgc acccttatca aggaccgcac tcaaaaattg
     7021 gaaggcggcg ttgaccggct ggtttccacg ctcaagagtt cgttgccgga agaaacgcaa
     7081 cgggcggtcc ccttccagcg cagcaacctg ctgattggca caacggaaga cctcccgcgg
     7141 ctcgacggcg aaatcaacaa ggccgtccaa gtcgccaacg cggtattgca gggctggaac
     7201 gataccaagc aggatttgga aaacacccgt caagatttgg aaaacacccg cgcggaactg
     7261 gccaacaccc gcgaacagtt ggaagatgcc cggacgcaaa tcgtgcaact gaacgatacc
     7321 attcgcgcca aggatcagga actgcaggaa aagagcgatc tgattgccag cctcgaggaa
     7381 gaaaaggccg ccctgaccag ccaggtagcc gatctcgaga accaggtggc cagcctgcaa
     7441 gaccagattg ccgaactgga agaagacaag gccttgctcg aaacacagct cgaaaagtgc
     7501 gacaaggagc tgcataagga aatcacgatg aagcccggca ccaccggcac catcgtgtat
     7561 gccaatgctg aatggaattt cgtggtgatt gatatcggct ccaacgacgg cgtgcagccg
     7621 accgccgaaa tgatcatcca ccgcggtgac aacatgatcg gcaagatcaa gatcagcgcg
     7681 gtgcgcgaca acgtgtccat tgcggaagtg atgagcgagt accaaaccgc caacgtgaag
     7741 gagggcgacc atgttctctt ctaatggcct gtcggcgatg acattgatcg ccttcttgct
     7801 gctggtggcg gtgatcggct tgcaagtggc tgaaatccac cactacagcg ccatcccgtc
     7861 gctgtggccg tgaggccatc cggcctttgc ctgacgctgc ttctcctggc cttgctggtc
     7921 tggagcggcg cgggctgcgc gaccccccac gacgaatcgg acttgccgtg gaatatgccg
     7981 cagtcgtggg aaagcgcccc cagcctgccc tttggcgcgg ccggcggtta ttgacgctcc
     8041 ccccgccttc accttacagc gggaaatccg ctccgtaggg caatggcaca ttgtgccgga
     8101 tggataactc ccggatttcc cggatcgtat ccgcgcggcc agtggcgatg tggtcgttga
     8161 tcagtttgta gattttctgg gcaacccact cgcgccgcaa accctcgcca ccttccgtgg
     8221 catccgccaa atccagcgca aaaaccatgt cgcgctgctg gccggcggct tcgcccaaaa
     8281 tttgcaacac ggctgttttt tgattggccg ggcaacgccc aaccaagcgc aacgggcgat
     8341 ccagatacag atgggtcaat gtggccggat atacttccgc gccggagtcg ctggcaaaac
     8401 ggtaggtcaa atcagccagc accggccgcg agagttcgcg ggcggcgcgc ttgaccgtgg
     8461 cggggatatc ttcacgcagg ggcctgaacc aggaatcgcc gcgattattg tgacccagta
     8521 aatccagcaa gaaggtgttg acccggtcgc ccgcgccaat ggtaaacacg gaaatttccc
     8581 ctttattggt ccggctgaaa cgagcgatga tgtccgagct gtccactgtg ccgagcgtgg
     8641 ggcggccatc ggtcatcaac accacaatca ttggccggcc gctctgcttg ggcaacttca
     8701 acacttcctg caaaggcagg aataaatccg tttgtccacg ggcgcgcata ttgtcggtaa
     8761 atcgcatggc ggcggccagc gattcgctgg tgaccggctg ccaatctggg aagcaaagag
     8821 tcggtgagtc gctatagcgc atcacattga cccggtccgc cgtggtcagt gtgccgaggt
     8881 aggattcaat gccatccttg aagaaatcca gcttggtgcg ggtcatgctt tcggagcaat
     8941 cctgcaccag caggatatcc ttgggcatga cgggcaaggc ggccaccccc gcccgcgcga
     9001 tggacaactc aaaatagagc gcatcttcgt cggaagcctt gaaggtggac acggccactt
     9061 ccagcaattg ttcaatcggt tggatatcgg tgatatcggc cgccttttca tccaaaacgc
     9121 tgccctgctc atgcaggcga tcggcctcgg ttccggcgcc tgcattggca gcagcaatca
     9181 tgtctgacgc cgaaggggtg gcttgactca acacagccaa agcctgcact gaactgtccc
     9241 cacgtccttg ggatgctgct gcggcggcgg caatggcctc tgcggttgcc ggcagggtga
     9301 tatctggggc agtttccacc cgctccacgg ccggcagcgt gaagcgaggc aatgcggcga
     9361 gttcatccgc ggctacgcgc tgctcgattt gcaggatatc ctgccggaaa tcaagcgcac
     9421 tgcgttcggc cggcggcgcc gcttgtggcg gaatggcttc gctgatcggc ggagcctcaa
     9481 tttccacgcc ctcggccgcg atttgtagtt cttccagcag gcttttttgc aacggctcaa
     9541 ttgttgcgaa gttctccgga ttttccggac ggaacttggg cggctgtgtg taggctggca
     9601 ggtcaggcag ggcacggact tcccccaaca ccagcgggtg cggcttgagc ttgtccggcc
     9661 aatcggccgg gcggcccaag gacatggctg ggaactcaat cagcaacacc gcatgcaacg
     9721 ccaacgacac caccaacgcg gcggcgaaca gcgctgcctg aacgagaggc tggcgcgatt
     9781 cgcggcggct gatgggctgg agactcaagg acgacatcgc ctacccctgc aacgtcatca
     9841 ggaattccag atttgtcttg gttttcttga ggcggccaat cagcagctcc atggcttcca
     9901 cggctggaac gccgttgagt gccttgcgca ggacccaaac acggctcagc tcatcggggt
     9961 gcatcaacag ctcttccttg cgggtgccgg acttctcgat gttgatggcc gggaagatgc
    10021 gcttgtccac gaggaagcgg tccaggcaaa tctccatgtt gccggtgcct ttgaactctt
    10081 caaagatcac ctcatccatg cggctgccgg tatccaccag cgcggtagca atgatggtca
    10141 agctgccgcc gttttcgatg ttgcgggccg caccgaagaa gcgcttgggc ttgtgcaggg
    10201 cattggcatc cacgccgccg gacaggattt ttccactatg cggttgcagg gtgttgtatg
    10261 cgcgcgccag acgggtgatg gagtcgagca aaatgactac atgcttgccc gattccactt
    10321 ggcgcttggc catttcaatt acgatttccg cgatttgcac gtggcgttcg ggcggctcgt
    10381 cgaaggtcga ggcaatcact tcagcctgcg tattgcgctc catgtcggtc acttcctcgg
    10441 ggcgctcatc aatcagcaga atgatcaact tcacttcggg attattttcc gaaatgctgt
    10501 tggcaatcat ttgcatcaga accgttttgc cggtgcgcgg cggggccacg atcagcccgc
    10561 gctggccctt gccgatgggc gtcagcaggt ccatgacgcg catgttgatg ttgtttttgc
    10621 tcttggtttc cagcaccagc cgcttgttgg ggaacagcgg cgtcaggttt tcaaaaggaa
    10681 tttttccccg ctgcccttct ggcgggcctt cgttgatgct gctcaccttt tgcagcgcaa
    10741 agaagcgttc cttgtccttg ggcgaacgaa tttcgccttc gaccagatcg ccggtacgca
    10801 gcgcaaagcg ccgcacctgc gaaggcgaga cataaatgtc atctgggcaa ggcaggtaat
    10861 cgttgttggg agagcggagg aaaccaaagt tgtccgggag gatttccagc acgccgcgca
    10921 caaacatggt cccgttgcag cgggcattcg ccttgaggat ttcaaaaatc agctcatgct
    10981 ttttgagcgc gcccaaatcc acgagctggt agctatcggc caactctttg agttggggca
    11041 cggtcaactg ctgaagctca ttcagcttta gcacgcgcgc catcttggcg cgttccgcgg
    11101 cggcggcttc ggccgctgcc cgctgagcgg cgatttgcgc ttcgcgcgcc gcttgctctt
    11161 ccggggtcag cggtggccgg ggcgggcggt tatcatggtg cggttggccg ccgtgccgcc
    11221 gattgcggtc gaagtagcgg tcatgacgtc cccctccccc gccctgctga taatgcggtc
    11281 ggtggtgctg ggggtacgca ggatggccac cattgccatt ttgctggcca ctggacaccg
    11341 ccgcatcggg cgcggggggt gccgggggct cgctttcgcg gctttcatac ggcacaaagg
    11401 gcgccggcgg tggggccatt tcaggtgcag caggctcggt gccggccaca ggcgcggtag
    11461 cttccggctg ggccgccgga gcatcgcctg gatgaaactc ttcaccatcc gcgatggtgg
    11521 tctccagcaa ggacgagggc aacggcattg ccgggcgctt ttgatcgtct tcgccggctt
    11581 ttttgcgggg cgggcggcca cggcgggctc cgccggcggc gcgcggttga cggggttgtt
    11641 tgggatttgt ggccatattc agtttccttt ctcaactaga ttcttccaga gcgctaggct
    11701 cttggcggtt aattcggcaa gggtgccatc attttcaatt atatgggtag cgcgcgcttt
    11761 tttttcggcg ataggcattt ggctggcgat gcgcgcatcg gcctcggcag gcttcaaacc
    11821 gcgggcggtc aggcgggcgc gcatggtttc caacgttgct gccacgcaca ccacggcatc
    11881 ccattcgtca tccatgccgg cttcaaacag caagggaatt acggcaacgc ccttgcggtg
    11941 ctgttggcga accgactcac accaggcgcg aacccgtgcc ttgatgggtg ggtgcagcaa
    12001 agcgttgagt cgcgcgcgcg ccgcaggatc ggcaaacacc tgggccccga gttgttggcg
    12061 atctacccca ccctgcggcg tgcgtaaatg ggcgccgaac tcgcgcaaca ccgattcaac
    12121 atactcgccg cctggctcga ttaattggtg agccagttca tcggtatcca aaatgcatgc
    12181 accctcgcgc cgccaaattt cggccaccgt gcttttgccg cacgcaatgc cgccggtcaa
    12241 tgcgatacac aaacttgctg gagcttgcgg cttcatcacc atggtttggc cggcgccaaa
    12301 gggtcggcct cctcttcaaa ccaatcttct tcttccgcac ggaatccgct ttgccgtaat
    12361 gcgtcggcgt ggcgtagtac tgcttgtgcc tccgtgttat ccgcatccat gcgcaacgcg
    12421 tgggcggctt gcaactcggc tggggctagt tgtccgtttt caagcgcgat gcgggcgcgt
    12481 tccgccaaag tctcggcaag gcgcgccgcc accgggcttt gcttgaggcg gtacacccgg
    12541 tatttccggt tggcaaactc cggcgcaaag aactgcaatt cctcgggccg ctgttcaaag
    12601 agccgggctg cgacgttggt ggccggatca agggcatcca ccatgtagcg catttgcaaa
    12661 ccgggttcga tggcggcaaa ctcacccatg gaatgcacat aaatggttgc gccctgggcc
    12721 tccatccaat cacggaattc tgcttcgtcg cctttgaaaa gcgcctcgcc atattcccgg
    12781 actttgcgcc gaatagcggc ggactcaaac ttcggatgga gcaccaccgg gcacccacca
    12841 taggtggcaa tggcggcgct aatgccgaag ttcgccaaca ccggttccgg cgcgtcatat
    12901 tttcgcaagt gtttcatcaa agcatcggtt tcagcggcat acacattggg gcggccccag
    12961 aacaaggaac cagtccattt cttggcttcg ggcgcctcga acgctttctt ctcccgtacc
    13021 agcggaccca accaaggttg cgcagcttcc aaaagcaacc cgcataccag cacactggcc
    13081 attacgccgc gccaacgggg attttcgcca caggatatct gccctgccca caaccccacc
    13141 aagccgcagc caaagatcgc cacccaaacg tggaaccgga aaaataaaat aaacgtgaaa
    13201 aacgaaacca gaaaatagaa tagaagaggg aagaaaccgg cttggtcgca acgggtgcga
    13261 aatgcaattc gtaacaaacc ccaaatggcc gccagcgtca acaccaaaag cgcggggaac
    13321 cactgccaga gcaggagcca ggaggttgaa ttgagcgcgg gcgcccacag gatgcgctgt
    13381 tcaaacgtca gcaaggccgg atcggtgggc cggatattga agaagcgcag tttggcccac
    13441 agcagagagc caaagtggct gtaggccggc agatacaaac cggcccacag gctggccagc
    13501 aacaacggca gcaggcccaa gatgactcgg gtggccttgt gctggcgttg ggcaatcggc
    13561 gcaccggcca acaacatgcc ccacccaagg gccatcgccg gcgaaaataa aaagccgtgc
    13621 gaccgcaaat aaggattgcg cagcccggca gccagcaaaa tggccatcag gggaatcatt
    13681 accgtccata gttccgtttt gcccaagcgg ccacggatgg cctcaaccaa gtgccaagcc
    13741 aagaaaagca ataaataaaa ctggatcaaa tcccacgcgc acagcgcagc gcctaccaat
    13801 aatgccgcca accacccccc tgccattttg ctgcgccagt gtaaagcttg ccgggaccag
    13861 gccagcacgg ccatggacca gatcaataaa ggcagtgcat tattttcatg cgatagttcc
    13921 tggcccgtcg agcgtgccac cgcgccaatg gctactgcat aaaaagccgc ggcccacaac
    13981 ccgccacggc cccccccgcc aagccagcgc acccagaaat acatgccggg aatggccaaa
    14041 caaaaccaca aaagatgcag ccagcggatt ttctctgcca gcgggaaagc tcccggccat
    14101 agctgtgccg cgcgcacata cagtttttcc acgccgatgg tatccacttc gcgcgagacc
    14161 acaccttgcg gatattgaat gccgcggtcc acagcgggca gtgctccatc gcgatagacc
    14221 atttgaatgc ggcgaaaagc caaagcactt tcgagcgtaa aaggaatact gccatcgctg
    14281 gtcaattcgc gttgggcatt caacacgtgc cgccgggtag ccacaccgcc ggcataaagg
    14341 cccaacagca gcacgcccaa cagaacgatt ttcagccacg gacgacgcaa catcagaaca
    14401 attccaattg agtgttaccc ggcgtggcag gcggcttcaa agtcgttttt gggggtaccg
    14461 ccggttgcgt ggacaattct gccgcaaaac gatgcatctc gaacttgttg aagaaatcca
    14521 gcaactgctg gcgatcaggc gccggcggag caatatccgg ccattccgga acgcccggca
    14581 ccgccgtatc caacgtcatt agttccacgt tcaatttggc cacatccagt tccgcccgca
    14641 gtttctcgcg tacgccgccc ggctcgatgt tgtcgagttg cgcggcgcat tgggccagcg
    14701 atccatattg ccgcaatagt ttggccgcgg ttttgggacc gataccatgt acgccgggaa
    14761 tgttgtcggc gctgtcgcca accaatgcca gccaatccac gatttgctcc ggccgcacgc
    14821 cagttttggc ttcaactccg gcagcgtcca actcttcttc ggctttggtt ggcggcacga
    14881 tgcgaatatt ttcgccgacc agttgcatca aatccttgtc actggtggcc aagcgaacgg
    14941 tcccaccctg tttggcggcg cgtacggcca gtgtggccat gacgtcatcc gcttcctggc
    15001 ctttgatcag gacaaggggg atcccggcgg cggcgagata ctcgttcaac aagggcaatt
    15061 gtgaccgcaa ggcatccggc atgggtgcgc gctgggcctt gtattccggt agtttttcca
    15121 gccggcgggc cggcgaaccg ccgtcaaaca ccacaaccaa cttgtcgggt cgccactttg
    15181 cggccagttg ctgtaacatc cgaataaacc caaacaaggc gttggttggc tggccggcgc
    15241 gggtggacaa gtcttgcagg gcataaaaag cccgataggc caccgccgta ccatctatga
    15301 tgagcgagga tgaagacata attgaaaatc aaccgctgtg cgcggggact aaaacgcaac
    15361 aataaccccg tcacacaaac ccgacgaaac gcaactggcg ttcgccggtt gggaaacctt
    15421 cgaaccaaac tgcacctccc ctgccctctt gggcaggaga gatgatgagt ggtgaaaaat
    15481 tattcgacgc tggcagtacc ggcatccaat ccggcacccg gcatcaccat attgctttcg
    15541 gtatgaaccg gccggccggc aggatccacc aagcgagccg tcacgaagat caacagattc
    15601 ttcttctggc tatagtggcc ttccgagcgg aacagccagc ccaacaccgg gatgtcgccc
    15661 aagatcggaa ccttatcctt gatggtggtc atttcttcgc ggatcagacc gcccatgacc
    15721 accgtttggc cgtcccaaat cacgatggat gtcgtgacat tacggctggt aaacaccggc
    15781 atgggcatgt tgaagaaata gaggtcgctg ccgctgatcg tgatgctgga accgtattgg
    15841 atccagtcca ccaattcggc cacttccggc gccatgacca agtcaatggt atagccgtca
    15901 ggaccaacgg tcggggtcac gttcaagatg acgccggttt cgcgtgtctg gaaggtgccg
    15961 ggggtcacga ttggcgccat ggtgacgtgg ccgtcggtgt ccacggtggg ctgcgtcaga
    16021 tcgaattcag tcgggtaaat gatttcgcgc acgacttgaa tctgggcgtt gacaccgctg
    16081 cgggtggtca cgcgaggtgc ggacagcagg tccgtgcccc cgtgctggga gagggcttgc
    16141 agcaccacgg tcacttccgg attggtcaac acgctggagg cgctcaggat gccgccgagg
    16201 ggggtctgcc ccgccgcacg ggtaatcgga gaagctggcg aagtcagata ggtatcaatg
    16261 ctgtaattaa agaaccggtt gccttgtgtc aggccgttct tgttggcatt gacttgcatc
    16321 cgttggccgg aaccatcgtt cttggtggcg aactcataat tatcgttcag aatccactgc
    16381 aggccgagtt cctccagatc atcctggttc acctcaatga accgggcttc gatttccacc
    16441 tggttcggca ccacgttgag ccgttggaga atctgctcaa aggtttcgag gttgtcggcc
    16501 gtgttggcga cgatcaactg gctgatggtg gcgttatagg tcacggaagc gcccgcgggg
    16561 aacttcaccc cggttttttc gaagaattcc ttgatgtccg acttggtggc cgtgctgcgg
    16621 tcacccatgg acacaaattc agagctttgg cgatcatcgc tggcgctctg gcgttcaaat
    16681 acaacatcca tgaacgacgg ctgaacggga tacatgcggg tgatgatgtt gccgacgggg
    16741 gtatccaccg aagtaatgat aacggcggcg tcctccacgc ggtacttgag accggcgact
    16801 tcggtgatgt acttaatggc atccagcatg ctgatacggc gcaggttaag cgtcacatca
    16861 cggactcctg gagcgccact ggaggccggc atggccatgt caaaatcact agcgccaaaa
    16921 ccccactcat ccgccgccgg ggccggagct gccgcggccg gggccgcacc gccgccaccc
    16981 gaaccgaggt tcaagatgat gttgacgcct tctttttccg gatcggccgc cacactggca
    17041 tccaccaaga agctgaccac atcattgatg ttggcctgac ggaactcaac ggctgggatg
    17101 atgatttcct tcatcttttc ctgcaaacgt tgggaagcag gcttagtatt cacaccggca
    17161 ttttcgccgg cctgatcgag ctgagccact tccagccggg tcggcgggct ccaactgcgc
    17221 cgcacatcgg tcatcatttc ctgcacccgg gcatggcgct cataggtggc cagtttgaac
    17281 ttgtcttctt cgagcttgcg gaggtagcgc atggcgctct tgtgatacgg atccagcttc
    17341 aggatttgtt caaaagccgc tttggcgcga tcataatcct taagcatgta ccactggcgg
    17401 gcttcgtcat acagcttgcc gatatccgcc tgcttctgga gagttgccgg ctgttcggca
    17461 ggcaatttgg gacgggcaat ttcggcttcg atcttgtcga gcttcttgga tagcgccagc
    17521 gcaccacggt gggccggatc gtttttgagg gcggaatcca ccaaacggcg tgcttcctca
    17581 ggacgttcct tcttttggac cagggcatga gcgcgcagat attgcgcttc ggccaggccc
    17641 ttcatgattt ggtcgcggac agccgcattc tgcgggcggt ccggcagatt gatcaatgcg
    17701 ctgttgagcg cagattccgc cttggcatat tcacctttgc tgagtgcggc gaagccggca
    17761 tcggaagcct tgagggcatc ggcttcggca gcctgacggc ggagttcttc ctgttgggcg
    17821 gtttctttgg cgagtttctt caattccttg ggatcaagtt tttcagccgg tttggttgcc
    17881 ttggccctgc tgggggtgac cacggtgccg gccggagtaa attccgccat ggtggcgtca
    17941 tcgtcatcgc cataatcagg agcggcaaag tcatccgtgg cagcaaccgc aggttcatca
    18001 aagccggcaa aatcgtccgc aggttctgcg accggctcag cagcaggcgc ctcaccgaac
    18061 aggtcgccca agaggtcgtc agcagcggcg gcgggagctt cgacaacatc ggcaggcagt
    18121 tcttcttccg caagaggagc agattcatca gcaaacaagt cggcgctgct gtccagatca
    18181 tcggcggcga cttcttcggc ctcaaccgcc ggctcggcag cctgatctgc aaatggatcc
    18241 gcttcggcca cgggctcttc ggcgggcgcg gcaaattcat cggccacctc tgccactgga
    18301 gtctcgctga cgaactcatc ggcaacggct tcggcttcct cagcaggagc ttccgcaacc
    18361 gccacatcgg cggtggcatc ggccacgctt gcggtggcgg tgtccgcatc acccagaagg
    18421 gcatccaaat cgaacccatc gtcctgggcc aatgccacac tgccaaacaa gagagctact
    18481 gtcattgccg caaccatgca ccatttcatt tggaactcct ccttggaatt gaaacgcggg
    18541 cgatgattcg cccaaatatc atccaaccac tgcctgttta aaagtttcat gacccaatca
    18601 ctgtcccggt gcagccgtca aaagatttgc tgccgccgcc gatggattca gtgcgtcttt
    18661 ctctgcggaa gtcactacgg ggacggtcac cttatcaccg gttgttgttt cctgaattac
    18721 tacactcgcc ggctggatgt caatgacctt gtattccttg ttccggaagg cgaatacgtc
    18781 gttgaggcgt ttgggcggac gcagcggctg gcggtcaatc aagttgacga aaagaatcgc
    18841 caattcctgc tcggtcacag ggcggccctt gacaagacgc acagcatgtt tgtcggcttt
    18901 gcgcaccaag atgatgctct ccccttcggg cgcggtcggg ttgtacttgg tgacctggta
    18961 accgagcaca tcatcctcaa gcttcacaaa gtaggtgcgt tccagtgatt gcaaattcaa
    19021 ttggaagcgc gtggagccat cgccaaacaa tgaggtgctg acaaagcgca aatagaacgg
    19081 cacgggccgg atggccgcca cacgcagttt cacgatcgga tccgggaagt tttccgggtt
    19141 tctggggtcg gttttggcca ggtattcctc aaggttggta aagccgtcat tgtccagatc
    19201 ccctgcggca tcagcaggat cctgcgggtt gagccccaac gccaactcga ccttgtcggg
    19261 tatgccgtcg ccatcggtat ccactgtatc cacattgatg atgggaggct gttctgcgag
    19321 gcagaaggga caggagagcg cgctataagg aatgggcttg ccacatttga tgcaagccac
    19381 gcgaacttcg ctgatggtgc tacgctccgg aaccgttaag gccaccgttg cttccttgcg
    19441 ggcgctgtcc agagcttctt caaacggcag cgtatccttg atcttcaccg gctcgccctt
    19501 ccagccaatg cgggccaagt tcggcttgtc gtggtcctta tccgctagaa tccactggct
    19561 caccaagatg caggaaataa acagcgccac caacgcgacc aggaaaatga tcttttcata
    19621 atggtcgcgc aaccaatcgt ttttccctcg gagtgcttgg ctcatgactt ctcctcgcct
    19681 tgcgctggat ccgcggcggg ttccagaaag cggtacatat ccacatccaa cacaatggtt
    19741 ggcaaatcgc ggccggcagt gacgcgtaaa tcgcgcggca gaatttcaat ttcaggtttg
    19801 ggcggtggtt gcaacgaacc gggcgcagcc cagccggcgg tgctgaccgg cgctgcggcg
    19861 tgttcttctg gcttcgtctg tggcggggtt accaccggct ttgcgaggtt ggtaatttcc
    19921 acccgcgtca ctacggtgaa cggcattccc ttggcgaggc gattaagcac ctcccattgc
    19981 gcctcatcct tggccttgta ggtaaattga taatgttcct tggtatagag accgagggga
    20041 tccacctgca agtcgagttt gggcgcttgc acctcagctt cttcgcgccc gcgggtgcgc
    20101 cgaccgtagg attcttcaac aggcgccggt tgggcatcgc gctcaaacac ggtgcgctcc
    20161 acggccacca gttcgctgat gccggctttg aagagcacat cgcataggat ggcgatggac
    20221 ttgaactggt ccaccagccg ttccatttca tcatccggcg ggaggttacc ttccgcatag
    20281 cgttggaagc caaaagcaaa gttggccggc agaaccacgt tgcgcttgcg ggcctcatcc
    20341 gtcagttcgc gcagcttggc ttcggccaac tggcggaagc ggtcgcgagt gaggtcaccg
    20401 acgggcgtct ggccctgctt gagcacgccg agcagatcgc cgagatatgt ctcaaacacc
    20461 gccttctgtt tgctgaggat gaccacattg gtatccgccg ggaaaggatt gcggttggtc
    20521 aagttgcgca actttagctg ttcacgttcg agattcgtgc ggcggtccgc gtagttgctc
    20581 ttggcgctga acagcagata gcccagccct agcaccacca cgaggaccag cacgcccccg
    20641 atcaccaatg gcatatactt gcggttcata gcacctgcgg ctccttcaag acggcgagga
    20701 ttttgaattc cagcgtggcg gaatcgccgc gggcgggcgg ctgccaaatg atttccgttt
    20761 tatccgagaa ccatggagat tcccgcaact ggtcgcggta ttgcccgatg gcttcgctct
    20821 tgattttgtc ttggtacgcc atgccttcga tctcgatgcc ggccacttgg ttgctcagcc
    20881 cggaatcggc ggcttgggga ttctgggtgg cgggctggtt ttcacccggc aaggtggcga
    20941 gcggatgcac gcgggtcaac cacatgcccg gcggcaaagg cgtgctaatc gcacccaaca
    21001 cgctgcgcca cgtgctgcgc cgggattcca gcgtggtcag cgcggcaatc ttcttttcca
    21061 cttcgcccac ggagctttcc acaccgcgca attggttttc cacgcgagac aactccccca
    21121 cccgcttcgc aatcagttcg ctggtgcgat cagccacatt ggccatccgg gtataaaacg
    21181 cgacccacac cgccaacgtc agcagcagca cgccggcagc catcgcaaaa atgggcagct
    21241 tgcgcttgaa cttcttgtct tcctccgctt taggcggcag caggttgatc tcaatggggc
    21301 atgaatgcag ttggcgcagc gccaagccaa ccagcgggcc catctcgcag gcgtgcgcgc
    21361 cgatttgctc cgcggggatt tgttccgcca cggccacgtt gttgaacggg ttgaaataat
    21421 cggttgcgat gttcagcttt tctttgagga aggtgtcggt gtagggaatc accgagctgc
    21481 cgccggtcag cagcagcaat gccggttgct ggccgccctg ttggctgcga tagaaattga
    21541 ttgagcggtt cagttcctgg tgcagccggg tcatcatgct gcgcacgctc ttggaaacct
    21601 tgtccacggt ttcgctctgc gggccttcgt aggcgccgcc aaacgccaca taggcgtggg
    21661 ccttcttcat ggcttcggcg tcatcaaacg agatgctgaa ctcgttcatg atggcctgcg
    21721 tgatctggtt gccggccact ggcacgctgc ggctgaaaat gcggccggct tcaatgaaga
    21781 ccaaatccgt cgagcgcgcg ccgatgtcca ccacgagggt gcagggcggc aggttgtcgt
    21841 agttgtagcg caccgcgttg tagagcgcga tcggcgccat gtccaccaga tccggcacca
    21901 atccggtgaa gcgcacacaa tccaccagtt cattgatgat atcggccttg atggccgcca
    21961 gcatcacatc cacatcgccg gttgcgccgc tgatcaattg gtagtcccag accacttcat
    22021 tcatcggaaa tggcacattt tgctgtgcct cgtactgaat gatctggtac accttctccg
    22081 gctcaaccgg cggcagcttc acaaaccgcg agaacactga gtgtcccgag atggccacct
    22141 gtaccggccc cggtttgcaa ccggattcgc ggatcaggtc attgatcgtg gtgatgatgt
    22201 attgggagcg gtcggcatcg ctctgcgagt ccacgccgag gtcgctgacg gcatagcggg
    22261 tcatttccaa cccgcctttc acgtccatga actcggccaa cttgaggcta tgcgacccga
    22321 tgtccagcgt cagtatgggc tttttggcca tggggaatcc ttgccggggg gttagcgcgc
    22381 gggggcgaca ggtttgatcg cttcgaactg gtcatagtcc agataggcaa acggcgtctg
    22441 cgaacgattc aacagcacgc catccacggg actgaaacga tcccaccaat tcggggtttg
    22501 cgcggtggac tcggtggcca ccacagcccc ccgggccttg accaccacgg ccacctgctc
    22561 aaccttgccc agccgggcca atgtccgcgg atgcaggaaa atatcgctat agtgccggcc
    22621 cttcggcacg ttcacatagg tgacggtggc gcggaacatg acctcagcgc ctttgttgct
    22681 ttggtcgcgc atatatacat aataagtaaa ctccagctca tcgagccagt ccggggccgt
    22741 ttcatattcc accaaatgcc gccaccagtc cggttgctgc gcggaggagg tactttgcac
    22801 ctgggtcttg aacacggggg tcttggtgtt ggcgaccggt cccattttgc gaatccgcag
    22861 gatgtcgctg cccgccggca acgcatttgc catttgcgcc catgcggccc cggccaccag
    22921 ccaaagcgca acgaacaaca caaccccgca taccgtccgg actcgcaatg tttgcatcat
    22981 gttcaggcct cctacacacc gtgttgcgcc gccaaaacct tctgggccgg gtagcacaat
    23041 tttccggagt atactcaggc catttgattt ccgtcaaccg ccatttcctt ttcccccgtg
    23101 gaacgcttgc cacgccctcg cgggcagcgc gttatacaag gcggttgaag attcgc
//
LOCUS       DAOPNP010000029        22594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2848_length_22594_cov_9.183238, whole genome shotgun sequence.
ACCESSION   DAOPNP010000029 DAOPNP010000000
VERSION     DAOPNP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22594
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_22594_cov_9.183238"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1743)
                     /gene="gatB"
                     /locus_tag="PKI64_04065"
     CDS             complement(316..1743)
                     /gene="gatB"
                     /locus_tag="PKI64_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE00220.1"
                     /translation="MSDYIASIGLEVHVQLKTRTKMFCACPAHEEGEPNTAVCPGCIG
                     LPGALPVINREAIRKTVKVGLMIGSRISLYSTFDRKNYFYADMAKNYQITQNDFPLCI
                     GGGVTIQVDGQPKTIRVHHIHQEEDVAKSTHLDNSTEVDFNRCSMPLMELVTEPDMAN
                     ADEVMAFLTTLKQMLIYADISDCNLELGHMRCDVNSSVRPAGATKLGVKTELKNMNTF
                     KGIQRALHYELQRQIEVLRGGGTIAQETRRWDDVAGVTLPMRTKESAPDYRYFPEADL
                     PPIVLDEATIAAWRAELPEMPAARRARFVQEYGLPEYDAGVLTADKAIADYFEAAAKQ
                     AANPKAVSNWVMGELLRALGEQGQDITAAKITPAALVALIGLVEAKTINMPAAKEVFQ
                     ILFENGGDPAAIVQAKGLAQVSDTGAIEGFAAQAIAANEKVAAEYRAGKEPALMFLVG
                     QVMKLSRGKANPQLAAEALRRQLKG"
     gene            complement(1779..3251)
                     /gene="gatA"
                     /locus_tag="PKI64_04070"
     CDS             complement(1779..3251)
                     /gene="gatA"
                     /locus_tag="PKI64_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOE00221.1"
                     /translation="MNTSLHELGLLATAAALAKKEVSAVELATALLDRAAQVEPKLNS
                     LTWQERDVVLAAAREADTARAKGDTRPWLGVPLAVKDVLSVNGQPCTAGSKMLRNYVA
                     PYDATVIARLRAAGCVFVARTNTDEFAMGGSTATSCFGPTRNPWSPARVPGGSSGGSA
                     AAVAARLAPAALGTDTSGSIRQPAAFCGVTGFKPTYGRVSRQGCVANASSLDQIGPLA
                     RSVEDAAALYQAIAGVDPLDSTTSPRPVGGVLAAAQQARDLKGLKLGVPREYFIAELA
                     PELAQLTRAAIDQCAALGAEIVEVSLPHVSASLPCYAVLGSAEASASLARYDGMRYGL
                     RVDAPELMEMYDRTRAAGFGDEVKRRLLFGTLVLTSRYHADFYLRAQKVRTLIRQDFL
                     QAFTACDALLGPVTLTPPPRLDAAPDSASARQWGDYCTAGVNLAGICALSVPCGFTAD
                     GLPAGLHVVGPAFQDELVLRIGAAYQHATDWHQRAPTGLE"
     gene            complement(3248..3547)
                     /gene="gatC"
                     /locus_tag="PKI64_04075"
     CDS             complement(3248..3547)
                     /gene="gatC"
                     /locus_tag="PKI64_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOE00222.1"
                     /translation="MNPAEKSDPVDVRYVAGLARLALTDAEAAAYQHDLAQMLAFAAQ
                     LRQVDVANVPPLQVLTEAQSAWREDVPAPGLSREAALQNAPLVRHGQFVAPKVVE"
     gene            3702..4628
                     /locus_tag="PKI64_04080"
     CDS             3702..4628
                     /locus_tag="PKI64_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE00223.1"
                     /translation="MKTLVILGSGPAGLTAAIYAGRAGLSPLVLDGPAPGGPLITTPE
                     IENIPGFPDGVAGYDFIDLLRRQAEKFGARFQSARVDGFAPRAAGGAELQLDDGAKLA
                     ADAVIIATGADHRHLGLPNERELAGHGVSYCATCDGAFYRDKPIAVVGGGDTAMEEAL
                     FLTRYASQLTLIHRRDAFRASKIMAQRVLNNPKITIRWNSVVTELLTNAEGKLAGLRL
                     RDTQTGAESELPAAALFVAIGLVPATKPFAGVLPTDANGYLLADGVKTKLPGVFVAGD
                     VADSRYRQAVTAAGAGCSAAIEAMHYLEAQDA"
     gene            4625..6454
                     /locus_tag="PKI64_04085"
     CDS             4625..6454
                     /locus_tag="PKI64_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOE00224.1"
                     /translation="MSASRVRLTAQQKQIYLRLLARAKPCRGRLALAVIFGAMFGGSI
                     FGIFASARGGMSKIFGGAATRLGDAASEWVNQWYAGSSHHDGIVTALVLGLLLFFVIL
                     RGVGFFFSKYLIEWVAQYVIMGLRNEVFGKILHLPLLYFTGSRTGELMSRTINDSQLV
                     ERGITEVISDLIQQPFVLVGAAVALCLTDLRLAMLSLAIFPICVIPIAIFGRRVRRHS
                     KASQERIADLASIQQEAIGGAAIVKAFGMEEREKSRFFKHSADFFRRQIKITASRAVV
                     NPLMELFAAAAACAIFLYARRTGLDLPSLLIFLGAMVVMYDPAKKLSRVHLVIQQSSA
                     AAERIFAILDTEDNVQDLPGAEPLTEAVSEIRYDNISFAYGADKPLVLDRINLTARAG
                     ELIALVGSSGSGKTTLVNLLPRFFDPTAGRVLLDGRDLRGFTLVSLRRQIGLVTQDTF
                     LFNDTVFNNIAYGEPGATREQVEAAAKQAHAHDFIMAMPHGYDTVIAERGILLSGGQR
                     QRLAIARALLRNPPILILDEATSALDTESERAVQAALNNAMAGRTVFAIAHRLSTIQR
                     ADQILVLDKGRIAERGRHDELLARAGIYRHLHHMQFDHPTPTP"
     gene            6451..6750
                     /locus_tag="PKI64_04090"
     CDS             6451..6750
                     /locus_tag="PKI64_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOE00225.1"
                     /translation="MNSDEAHLLEALCAIRDPAEMRQVLADILTRAEYTALRKRWSIL
                     RLLRAGVPQREIARRIGGSLCNVTRGARLLRNPNCSSARLMERLEKANRLPTANP"
     gene            6809..8209
                     /locus_tag="PKI64_04095"
     CDS             6809..8209
                     /locus_tag="PKI64_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOE00226.1"
                     /translation="MKTRLALLALLLLSFVAIPTAPAATPPANAPVYVIPVHGQIEGA
                     LLYVLRRGLAEATAQQAAAVILTMDTPGGTLAAASDIVRTIQASPIPVYTYVEKDAFS
                     AGAIIALATKKIYMAPGSVIGDALPIMMSPFGGVQEMSGGIEEKAVSAVSALIRAAAQ
                     DAGHDPELAEKMVRRENEFKIGDEVICPAGRLLTLTADEAAKPGRDGRSLLSAGTAPT
                     LADVLAQLELTGHPQVAMQITAAEKVARLIAAAAPILMILGFLGIYIEIKTPGFGLPG
                     ILGAICLALFFWGHHIAGLAGMEDLVIFIAGCVLVLVEIFLIPGFGFTGVLGLALIFI
                     SLLGAMVRLAPGGPWLPAWPDVQIPLVKLLGSIVGAGAGALLLGRFLPRSPLFGKLTL
                     GAATSAAEGYTAAPDTSALVGQTGVTLNLLRPAGTARFGDDRLDVTTSGEFIPAGTPV
                     QIVEARGSRIVVQKIG"
     gene            complement(8226..10649)
                     /locus_tag="PKI64_04100"
     CDS             complement(8226..10649)
                     /locus_tag="PKI64_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOE00227.1"
                     /translation="MSKTEVVGASPVRVDGFAKVMGAAQYTDDLDFGPGLLHAVVVES
                     TKAHALLKKVDVSAAEKAPGVVRVVTGKDCPHKFGLYMQDRHIFAQDRVRFVGEQIAA
                     VVAETPEAALRAAKLVQVEYEPLPAILSLGDALKKDAVLLHPELGSYQRVPWFFPRAG
                     TNIAHWRKTRKGDVAKGFKQADFILEDTYTVPRYAHCPIEPHAVTGLFDLSGRLTLWA
                     SSQSPFAQRNVMAIALKPFGLSHKDIRIVTPYVGGGFGGKAGVSMEIIAAVLAIVMKG
                     RPVKLNWTREQEFMNTSQRQGVIARIKLGVKKDGRITALEHTLHWDAGASAEYGANVV
                     NAVGLSATGPYRVPNVKIDSVCVYTNMPPCGAYRGFGYSEFHFGLESHMTRVAKAIGM
                     DPVAFRKLNAIREGDTLAYGAHMNAGDLHGAIDRVAKEIQWGKAVRSQKRNMAIGKAV
                     ACFWKAPAMPPNASSAAFLKFNEDGSLNLSVSGMEIGQGFLTVMAQIAAEVLTVPVAK
                     IRVETPDTDRNPYEWQTVGSHVTWSCGNAVRAAAEEVKAKILATVARAKKLKVADLYL
                     EDECVKSRRDKAFSLPLRSFVIDGIMRPAGNFIGGPIQGTGMFMPEFTSAMGDPETSQ
                     GGHPNVHYTVGCAGIIMEVDKDTGKMSIRKVALAVDAGKAVNPDLVKGQVVGGVLQGL
                     ATALYEDIRYAPTGRLLNPNFTDYKIPTALDCPQEIVPIILETAQPDGPFGARGVGEH
                     TMIACAPIVADAIENAVGVRLKTMPLTAEKVALALRGIEYDDVKGDNLGFCFRGRPCD
                     YPFEVGGAK"
     gene            complement(10646..11137)
                     /locus_tag="PKI64_04105"
     CDS             complement(10646..11137)
                     /locus_tag="PKI64_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOE00228.1"
                     /translation="MKEITINLNGAARRVAVAPNETLLDVLRGKLGIKTPKEGCGRGD
                     CGACTVLLDGKVVRSCLILAIEVDGQSVTTLEGVGQQGPTALQQAFVANNAFQCGFCT
                     SGVVLAASALLTEKPQPTEHEVREALAGNLCRCTGYEPIVKTVLDVARKTGRKSAKRR
                     ARS"
     gene            complement(11134..12033)
                     /locus_tag="PKI64_04110"
     CDS             complement(11134..12033)
                     /locus_tag="PKI64_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOE00229.1"
                     /translation="MSIACEFDYAKPDSLSAALRLLAAQPGRVPLAGGTDLVAWLRDG
                     AVSPAFLVDIKGLPELRGIRLKNGRLWLGANTTFAEIIESPLVRKHAPILVEAAGMVA
                     SVGVRNRATIGGNICSAVPCCDSGPALLVYEATVHVATRQGAKNIPATKWFLGPRRTA
                     LAKGGLAVAISLPVGKHAGAFAKLKRYRGEDLAQASVAVRVDARGEWRVAYGSVAPTP
                     IRGPKVEKVLRGQKNPPVELLQKARPLAEAEVAPITDIRGSEVYRRLMIGIMLERTVE
                     LATARLAGRGADYGINVLEEACS"
     gene            complement(12030..13520)
                     /locus_tag="PKI64_04115"
     CDS             complement(12030..13520)
                     /locus_tag="PKI64_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOE00230.1"
                     /translation="MVIFHRGVQVSSRHLFLTIGDWLVIILAQLAAVFLRLGFADGGT
                     YMVENWGSLVSSALIYGLVFYAGGMYEPPTRSRRFDFDFLPLIVTGIAAGITGLVLYT
                     RPQMVLGRGIWALSSVLILILTYLLRRVFLALMRRGIFLKQALVLCDHPDRAAELLQL
                     VEDQPFSFYRVAGLVVCGTTPASAYTGKMPVLGTLDDLDRLVAEREIAVLLLSVSVMR
                     SRELLARLRPLRYMGVEIMDYVSLSEELAQEIPLAHINDEWLMTAALNSSVIQIRKVK
                     RATDIVVSVFGLLLGLPLIVLAGILIKLTSPGAVVYRQTRLGLGGKPYVLYKLRTMRM
                     DAEAQGAVWAGQRDTRVTKVGYYLRKWRIDEIPQLFNILKGEMSLVGPRPERPEFTEK
                     LARFIPFYDERHLVQPGLTGWAQVRFPYAASFEAAARKLQFDLYYIKNMGFLLDVSIL
                     LRTFKTILVGLRHEGDGSFLPVDDKLEDLLAGTNLPEGGESEGEQT"
     gene            complement(13549..15789)
                     /locus_tag="PKI64_04120"
     CDS             complement(13549..15789)
                     /locus_tag="PKI64_04120"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOE00231.1"
                     /translation="MRDMIQRPTTGNTPPPPETGPAESAPKGFDIRKYFYIITKRLWL
                     LLLCFMTAIVIMLITMARQVPEYQATCKILLTRSAVLPANLQRREAEAVGGDYAQTQC
                     NVIRSRDVLQTARAKLGMPPQEFGEKFRELSVYPLWQTAIIAISVTGLEPGFCADYAN
                     AIADAYLEYKSVEYDGSSQNTVDNLSRQAQDLAEKIASMEGNLLAFVRENSVVGIQER
                     GNVAANLLAQLSKQAAEHRTQRMLLEAQQPLLNQAPDEVVLAILEYGLPAPAVEVSKG
                     GLPAGDSPAVDSAESLIEHGVVAPPQWSTLKRENSILEAQLVAYRKKYKDEHPLIQET
                     QRKLQANEDALKVEVQFALKQYYSQLEALSIKEKAAQQVEQEWEEQALEIDRKQKEYE
                     GIQRNLKRLQSLYDLIFNRLQEIDISAGIQTESVKILERALAPGSPLKPRNMQSLFLA
                     ALIGLAIGLGLIFSLEFMDDSIRYPEEIGKALGANFLGLVPTAHWRQEDDSSYWLGNV
                     DPASGFAESYRNIRSALLLNPTGRPFRTLTVISSVPKEGKTTTSVNLGTSFAQTGHRV
                     LMVDADLHRGGLHRFFGLQAGRGLSEILAGQTTLDKVVQHTPQAGLDLVGTGTFPTNP
                     AELVMRREMREFLAEASEKYDLVILDAPPALAVSESTVIAAQTDGALLVVWGGRTSRK
                     LVQASMRQLLSRGANVLGCVLNNLDLTRMGNYGYSSYYHYYGYDYRYEEDTSPVTPGA
                     GPAASS"
     gene            complement(15792..16853)
                     /locus_tag="PKI64_04125"
     CDS             complement(15792..16853)
                     /locus_tag="PKI64_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOE00232.1"
                     /translation="MAGDRLNISVREQPDMNRVYAVAGDGSIDVGFAGRVVIAELTAD
                     EAARRLESVLEDRYFKEANVTISVANFVEGDVLLTGAVQSPGSIPFRGDSLLTLMEAI
                     TRAGGLREDAAGDRVRILRWTPGGSMERQTVEVNVQKMLTTLDFSDDQYLRPRDMIIV
                     PARGLEEGRDEFLALGEVGSPGFHPYVPGLDIIKVVTMVGGLGEFADWSGARILRPRA
                     SGEYALVPLNLDRLFGAADMTMNLPVQKGDIFFIPSVRNQVRELVYLLGEVNQRGAVA
                     LSSGPQATAARVILSQGGMTQFANPSKVQIQRTAPDGSKKTMLVDVGHILKEGSFEED
                     VPLQDGDVIIVPEKGLLGL"
     gene            complement(17064..18497)
                     /locus_tag="PKI64_04130"
     CDS             complement(17064..18497)
                     /locus_tag="PKI64_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00233.1"
                     /translation="MREAAQSKAGGYPVTTLPCGHRRWAGWGLRAVLALAALFFWPGA
                     GLAQTMRVGNLDLTLVGTLSLAYDSNVDDIYPDDEKPGMIKDDFYLMPSLSIQSQTLT
                     MWPRITFNVSGMIGYEDYFRRNDLDTEVYNVAVNFQTAHPRLTLGGMVSVDYSVDGIE
                     DQYVPGGTSRDPVLTLDANAFANWNYRILRLEFSEEYSREMHRFEQYQAGDQEEITTT
                     ASIFLDVFRWGSLFYTWERVVTTQLQSEQETTEITQTFGIDGSIPVDILRRPKITYQF
                     GFSYEDEQVDQTAGREEPTWEPTHSITVSDEFDLSKSIHLSLSATWEDTWENSTPDFA
                     LPGQNKDNEDDEVTFQYNIKLTQLLGPRAQHSLTFTQEPEATFGSNSDTESTTYGYDF
                     SLRDLLVYGLNFSFSAVYELETPLGVPHPETEKTTTITTGLDHTRQLSRKLSRNLSYV
                     YTWENSNFHHGGANEKHLVMYTLIYAF"
     gene            complement(18563..18910)
                     /locus_tag="PKI64_04135"
     CDS             complement(18563..18910)
                     /locus_tag="PKI64_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00234.1"
                     /translation="MMRTASGILVGLVIALSLSACASWNVDVSKLQLGMEPEAVREAI
                     GKPFTVRASKIYKDQEYAEVWEYLPPAFTWTPKTYWVYFQNGKVVQWGEPGDFSGSTA
                     AAVREYNANKSDR"
     gene            complement(19040..20230)
                     /locus_tag="PKI64_04140"
     CDS             complement(19040..20230)
                     /locus_tag="PKI64_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOE00235.1"
                     /translation="MALKIDLDLCTGCGACVAVCPFCALTQRAEDGKVEVNEACTACG
                     ACVEVCPVGALAIEVAERQVSAEEQAAYRGLWVFAEQRAGRIQEVAFELLGEGRRLAD
                     KLGVELSAVLLGHQIQAGASALFEHGADKVYVVDHPALAAYTAEPYSEAMSALIKKYK
                     PEIVLAGATSIGRGFIARVAIQVSAGLTADCTELDVDVEKRLLLQTRPAFGGNIMACI
                     ICPDHRPQMATVRPKVFKQPTPVAGRTGELIVEPLTLKDPVVKVLEVVNAMENEVNVA
                     GAEFIVTGGRGLGSPENFKLLAELAEALGGAAIGASRAAVDAGWISSFHQVGQTGKTV
                     RPKIYIACGVSGQIQHLVGMNSSDTIIAINKDPDAPIFGVADIGIVGDLFQVIPELIK
                     ALKR"
     gene            complement(20243..21031)
                     /locus_tag="PKI64_04145"
     CDS             complement(20243..21031)
                     /locus_tag="PKI64_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOE00236.1"
                     /translation="MHIVVCVKQVPNTTQVRINPETNTLVREGVESIMNPFDENALEM
                     ALQLKEQHAGTKVTVLTMGPPQAAEVLKEAVGRGADAVVLLSDRAFAGSDTWATSYAL
                     SMAIRQMEKPDLILFGKQAIDGDTAQVGPGVADWLDLPCIAYARKIEVTNGVAQVERA
                     YEDGYEKLEVALPCVLTVLKEANVPRMASLRGKMAAKKLSIVPLTAEGVGADPAKLGL
                     NASPTRVVKIFSPPPKTGGVKWQGEEPAVMAQRLADVLRERKLI"
     gene            complement(21084..22250)
                     /locus_tag="PKI64_04150"
     CDS             complement(21084..22250)
                     /locus_tag="PKI64_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE00237.1"
                     /translation="MNYGLSEMALEIRGMMREFAEARVKPVRAELDEKGVFPTELLRE
                     LGKMDLMGLYIPTEYGGTFEGDSMPHIVAVEELSRICIGVSVSFAANGLGADPILLGA
                     SDEQKKKYLPPLASGEKWAAYGLTEPNAGSDAGSIRTTAVKKGDKYILNGTKQWITNG
                     GEADIYTIFAVTDKSRGARGVSCFIVEKGTPGFSFGKKEDKLGIRCSATRELIFEDCE
                     VPAENLIGREGTGFIWAMKTFDVSRPGIAAQAIGLAQGALDEAVAYAKVREQFGRPII
                     ANQGLSWMLAEMATKVETARALTYATVKTIDSGAKDISKFSAMCKMYASDIAMQVATD
                     AVQVFGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRDIIGLALNKEYGAVAKK"
     gene            complement(22311..>22594)
                     /locus_tag="PKI64_04155"
     CDS             complement(22311..>22594)
                     /locus_tag="PKI64_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00238.1"
                     /translation="AWVNAPGLDQPVERIAFINGEARNRQVFHADGLGSIAVLTDESG
                     EPVQTYTYAAFGSIRTQTGTDPFLGLSVTVACAQARRHLWIASAGGALA"
BASE COUNT         4248 a         7399 c         6533 g         4414 t
ORIGIN      
        1 tccacatcac catgatgcat gaacgaaacc ccatagcatc tacaatccaa tccgagacag
       61 gaaaaacgac gagctttctg aattatccgc atcattggcc tctgtaatca attatggctt
      121 tatcttccat gttgatgaat tattcaggct agagcatttg aagaaataat gtagccggga
      181 ggtcgtcgcg aattgggctt ggccgaggaa ggcgaagcag gaatcgcaac gctatttcga
      241 tgcagcctga caacggccaa gcccaatgca gtagacaaaa cggctacaga attgatgaaa
      301 atgctctaaa ccacatcagc ccttcaattg gcggcgcagg gcttccgcgg ccaactgcgg
      361 attggctttg ccgcgggaca gcttcatcac ctgcccgacc aagaacatca gcgccggctc
      421 cttgccggcg cgatattccg ccgccacttt ttcgttggcc gcaatggcct gcgccgcgaa
      481 accttcgatg gcgccggtat cgctgacctg cgcgagcccc ttggcctgca caatggctgc
      541 cgggtcgccg ccgttctcga agagaatctg aaacacttcc ttggcggcgg gcatgttgat
      601 ggtcttggcc tccaccagcc caatcaacgc caccagcgcg gcgggcgtga tcttggcggc
      661 ggtaatatcc tgcccctgct cgcccagcgc gcgcagtaac tcgcccatca cccaattgga
      721 taccgccttg gggttggccg cctgcttggc ggcggcctcg aaataatcgg cgatggcctt
      781 gtcggccgtg agtacgccgg catcatattc cggcaagccg tattcctgta cgaaccgggc
      841 gcgccgcgcc gctggcatct ccggcaattc cgcgcgccag gcggcgatgg tggcttcatc
      901 cagcacaatc ggcggcaagt cggcttccgg gaaatagcgg taatccggcg cggattcctt
      961 ggtgcgcatg ggcagcgtca cgccggccac gtcgtcccag cggcgcgttt cctgtgcaat
     1021 cgtgccgccg ccgcgcaata cttcgatctg ccgttgcagt tcgtaatgaa gcgcgcgctg
     1081 gatgcccttg aaggtgttca tgttcttgag ctcggttttc acgcccagct tcgtggcgcc
     1141 ggcaggccgc acggaggaat tgacgtcgca gcgcatgtgg ccgagttcca agttgcagtc
     1201 gctgatgtcg gcatagatca gcatttgttt gagcgtggtc agaaacgcca taacctcatc
     1261 ggcattggcc atgtcgggct cggtcaccag ttccatgagc ggcatgctgc aacggttgaa
     1321 gtccacttcg gtcgaattgt ccaagtgggt acttttggca acgtcttctt cctgatgaat
     1381 gtgatgcact cggatggttt tgggctggcc gtccacctgg atggtgaccc cgccgccgat
     1441 gcacagcgga aaatcatttt gagtgatctg ataatttttg gccatgtccg cataaaaata
     1501 atttttgcgg tcgaaggtgc tatagaggga aatgcgcgaa ccaatcatca ggcccacttt
     1561 gacagttttg cggatggctt cgcggttgat cacgggcagc gcgccgggca gaccgatgca
     1621 gccggggcag acggccgtgt tgggctcgcc ttcctcatgc gcggggcagg cgcaaaacat
     1681 tttggtgcgc gttttcaact gcacgtggac ttcgagtccg atggaagcaa tgtagtcgct
     1741 catggtattt ctttcgcggg gccgccgaaa atctgctgct actccaatcc tgtcggcgcg
     1801 cgctgatgcc agtcggtggc atgttgatag gccgcgccaa tgcgcaaaac caattcatcc
     1861 tgaaaggctg gccccaccac atgcaagccg gcgggcaagc catcggcggt gaaaccgcac
     1921 ggcacgctca gggcgcaaat gccggccagg ttcacgccgg cggtgcagta atcgccccac
     1981 tgccgggcag aagcggagtc cggcgcagca tccagccgcg gggggggcgt cagcgtcacg
     2041 gggcccagca gggcatcgca cgcggtgaag gcttgcagga aatcctgccg gatcagcgtg
     2101 cgcacctttt gcgcccgcaa gtaaaagtcg gcgtgatatc ggctggtcag caccagcgtg
     2161 ccgaagagga gccggcgctt gacttcatcg ccgaagccgg cggcgcgggt acggtcatac
     2221 atttccatca gttcgggcgc gtccacgcgc aggccgtagc gcatgccatc gtagcgggca
     2281 aggctggcgg aggcttccgc ggagcccaac accgcatagc agggcaggct ggcggacaca
     2341 tgcggcaagg atacctcgac aatctccgcg cccagcgccg cacattgatc tatggccgcg
     2401 cgcgtcagtt gcgccaactc cggcgcgagt tccgcaatga aatactcccg cggcacgccc
     2461 agcttgaggc ccttgagatc gcgggcctgt tgcgccgcgg caaggacacc gcccaccggc
     2521 cgcggcgagg tggtggaatc cagcgggtcc acgcccgcga tggcttggta gagtgccgcc
     2581 gcgtcttcca cgctgcgcgc gagaggacca atctggtcca atgatgacgc gttggccacg
     2641 cagccctgcc gcgacacgcg accataggtc ggtttgaaac ccgtcacgcc gcaaaatgcc
     2701 gcgggctgcc ggatgctgcc gctggtatcc gtacctagcg cggccggcgc caaccgcgcc
     2761 gccacggcag ccgccgagcc gccgctggaa ccgcccggga cgcgcgccgg gctccacgga
     2821 ttgcgcgtgg gcccgaaaca actggttgcg gtgctaccgc ccattgcaaa ttcgtcagtg
     2881 ttggttctcg ccacaaatac gcaccccgcg gcgcgcaggc gggcgatgac cgtggcatca
     2941 tagggcgcca catagttgcg cagcatcttg gagcccgcgg tgcagggttg gccattcacg
     3001 ctcaacacat ccttgaccgc caggggcacg cccaaccaag ggcgggtgtc gcctttggcg
     3061 cgcgcggtat cggcttcccg cgcggcggcc agcaccacat cgcgctcctg ccacgtcagg
     3121 gagttcagct tgggttccac ttgggccgcg cgatccagca gcgcggtggc caactccacg
     3181 gcggacacct cttttttcgc caaggcggcg gcggtagcca gcaagcccag ttcatgcagg
     3241 gatgtgttca ttcgaccacc ttcggcgcga cgaattggcc gtggcgcacc agcggcgcgt
     3301 tttgcaacgc ggcttcgcgc gagagaccgg gcgcggggac atcctcccgc cacgcgctct
     3361 gcgcttccgt gagaacttgc aggggtggca cgtttgccac atccacttgg cgcagttgcg
     3421 cagcaaacgc cagcatttgc gcaagatcgt gttgatacgc ggccgcttcg gcatcagtca
     3481 aggccagccg ggccaagccc gctacatagc ggacatccac cgggtcggat ttttccgcag
     3541 gattcatgat cttccttcgt gtatgccacc cccgtttcgc gggaactggc aaggggcacg
     3601 ttgtagcatt ctgccgcacc ttgcgcaata tctttgcccg aaccgcgcgc cgccgccgtt
     3661 tcccgttgcg gctgggagcg ttcgctggta tcgtaccgcc catgaaaaca ctggtgattc
     3721 tgggctccgg gccggctggc ctgactgccg ctatttacgc cggccgcgcc ggtttgtccc
     3781 ccttggttct ggatggtccc gcccccggtg ggccgctgat taccaccccg gaaatcgaga
     3841 atattcccgg cttccccgac ggcgtggccg gctatgattt tattgatttg ctgcgccggc
     3901 aggccgagaa gtttggtgcg cgcttccaga gcgcccgggt ggatggcttt gcgccgcgtg
     3961 ccgccggcgg cgcggaattg cagttggatg acggtgcaaa actggcggct gatgcggtca
     4021 tcatcgccac cggtgcggat caccgccact tgggcctgcc caatgagcgc gagttggccg
     4081 gccatggcgt ttcctattgc gccacgtgcg atggcgcttt ctaccgcgac aaacccattg
     4141 ccgtagtcgg cggtggcgac accgccatgg aagaggcgct gttcctgacc cgctatgcct
     4201 cgcaactgac gctgattcac cggcgcgacg cgttccgcgc ctccaaaatc atggcgcagc
     4261 gcgtgctgaa caaccccaaa atcactatcc gctggaacag tgtggtgacc gaactgctga
     4321 ccaatgcgga aggcaagttg gccggcttgc gcctgcgcga cacccaaacc ggcgcggaaa
     4381 gcgaactgcc cgcggccgcg ctattcgtgg ccatcggctt ggtgccggcc accaaaccgt
     4441 tcgccggcgt cctgcccacg gatgccaacg gctacctgct ggccgacggc gtgaaaacca
     4501 aattgcccgg cgtgtttgtt gccggcgatg tggctgacag ccgctaccgc caagccgtca
     4561 cagcggcggg cgccggttgc agcgcggcca tcgaggccat gcactatctg gaagcgcagg
     4621 atgcatgagc gcgtcccgcg tcaggctgac ggctcaacaa aaacagattt atctgcgcct
     4681 gctggcgcgg gctaaaccgt gccgcggccg cctcgcgctg gcggtcattt tcggcgcgat
     4741 gttcggcggt tccatcttcg gcatttttgc ctcggcccgt ggcggcatga gcaagatttt
     4801 cggcggcgcg gccacgcggc tcggcgatgc cgcctccgag tgggtgaacc agtggtatgc
     4861 aggcagcagc catcacgacg gcatcgtgac cgcgctggtg ctggggttgc tgctgttctt
     4921 tgtgatcctg cgcggggtgg gcttcttttt cagcaagtac ctgattgagt gggtggcgca
     4981 atacgtcatc atgggcctgc gcaacgaggt gttcggcaaa atcctgcact tgccgctgct
     5041 ctatttcacc gggtcgcgca cgggcgaact gatgtcgcgc accatcaacg acagccagct
     5101 cgtggagcgc ggtatcaccg aggtgatttc cgacctcatc cagcagccat ttgttctcgt
     5161 gggcgccgcg gtggcgctct gcctgactga tttgcgcctg gccatgctca gtttggccat
     5221 cttccctatt tgcgtaatcc ccatcgcgat ttttggccgg cgcgtgcggc gacactcgaa
     5281 ggccagccag gaacgcattg ccgacctggc cagcatccag caggaagcca tcggcggcgc
     5341 ggccatcgtc aaggccttcg gcatggaaga gcgcgaaaag agccgttttt tcaagcacag
     5401 cgcggacttt ttccgacggc aaatcaagat caccgcgtcc cgggccgtgg tcaacccgct
     5461 catggagctc ttcgccgcgg cagccgcctg cgccatcttt ttgtatgcgc gccgcacggg
     5521 tttggatttg cccagcctgc tgattttttt gggcgccatg gtggtcatgt atgatccggc
     5581 caaaaaattg tcgcgcgtgc atctggtcat tcagcaaagc agcgccgccg ctgaacgaat
     5641 ttttgccatc ctggacactg aagacaatgt gcaggatctg cccggcgccg agcctctcac
     5701 cgaggccgtc agcgaaatcc gctacgacaa catcagcttt gcttatggcg ccgacaaacc
     5761 gctggttctc gaccgcatca acctgaccgc gcgggccggc gaactgattg cattggtcgg
     5821 tagttccggc tccggcaaaa ccacgctggt caatctgctg ccccggtttt tcgaccccac
     5881 cgccggccgc gtgctgttgg atgggcgcga tttgcgcggc tttacgctcg tgagcctgcg
     5941 ccgccaaatc gggctggtga cgcaagacac ttttctcttc aacgacacgg tcttcaacaa
     6001 cattgcctac ggcgaacccg gcgccacgcg cgaacaggtc gaagccgccg ccaaacaggc
     6061 ccacgcacac gacttcatca tggccatgcc gcacggctat gacaccgtga ttgccgagcg
     6121 cggcattctg ctctccggcg gccagcgcca acgtttggcc atcgcccgcg ccctgctgcg
     6181 caatccgccc atcctgattc tcgacgaagc caccagcgcg ctggacaccg aatccgaacg
     6241 cgcggtgcag gccgcgctca acaacgccat ggccgggcgc acggtgtttg ccattgccca
     6301 ccggctttcc accatccagc gcgccgacca aatcctcgtg ctggacaagg gccgcatcgc
     6361 cgagcgcgga cggcacgatg agttgctcgc gcgcgccggc atttaccgcc atctgcacca
     6421 catgcagttt gaccacccca cccccacccc atgaactcgg acgaagccca cctgctggaa
     6481 gccctctgcg ccatacgcga ccccgccgaa atgcgccaag tgctggcgga catcctgacc
     6541 cgcgcggaat ataccgccct gcgcaaacgc tggagcatcc tgcgcctgtt gcgcgcgggg
     6601 gtgccccagc gggaaattgc gcggcggatc ggcggcagcc tgtgcaacgt cacgcgtggc
     6661 gcacgcctgc tacgcaatcc aaattgctcc tccgcccgcc tgatggagcg gctggaaaag
     6721 gctaaccgcc tgcccaccgc caatccgtag ccctgcgccg tccgctttgc ggttgcgggg
     6781 ggcgccctcc gcggcttaca gtagaggtat gaaaacccgc ttggctctgt tggcgctgct
     6841 gttgctctca tttgtggcca ttccgaccgc ccccgcggcc acgccaccgg ccaatgctcc
     6901 ggtgtatgtc atccccgttc atggccagat cgaaggcgcg ctgctctacg tcttgcggcg
     6961 cgggctggcc gaggccaccg cgcaacaagc cgcggccgtc atcctcacca tggacacccc
     7021 cggtggcacg ctggcggcgg ccagtgatat cgtacgcaca attcaggcct cccccatccc
     7081 ggtctatacc tatgtggaaa aggatgcttt ttccgccgga gccatcatcg cgttggccac
     7141 caaaaagatt tacatggcgc ccggttccgt catcggcgat gccctgccca tcatgatgtc
     7201 gccgtttggc ggcgtgcagg aaatgtccgg cggcatcgaa gagaaggcgg tttccgcggt
     7261 cagcgcatta atccgcgccg ccgcgcagga cgccgggcat gaccccgaac tggccgaaaa
     7321 aatggtgcgc cgcgaaaacg aattcaaaat cggcgatgaa gtcatttgcc cggctgggcg
     7381 gctgctgacg ctgaccgccg acgaagccgc gaagcccggc cgcgatggcc ggtcgctgct
     7441 gtccgccggc acggcgccga cgctggccga tgtgctggcc caactggagt tgaccggtca
     7501 tccgcaagtg gccatgcaaa tcaccgccgc agaaaaagtg gcgcgcctga tcgctgccgc
     7561 cgcccccatt ctgatgatct tgggtttcct tgggatttat atcgaaatca aaacgccggg
     7621 attcggtttg cccgggattt tgggcgcaat ttgcctcgcc ctgtttttct gggggcacca
     7681 catcgccggg ctggccggca tggaggattt ggtcattttt atcgccggct gcgtgctggt
     7741 gctggtggag atttttctta ttccgggctt cggttttacc ggcgtgctgg gcttggcgct
     7801 gatttttatt tcgctgctgg gcgccatggt gcggctggcg ccgggcgggc catggttgcc
     7861 cgcttggccg gatgtccaaa tcccgctggt caaattgctg gggtccattg tgggggcagg
     7921 cgcgggggcg ctgctgctcg gacgtttttt gccccgcagt ccgctcttcg gcaagctgac
     7981 gctcggcgcc gccacgtccg ccgccgaagg ctacaccgcc gcgccggaca cctcggcgct
     8041 ggtggggcag acgggagtca ccttgaatct gctgcgcccc gccggcaccg cgcgctttgg
     8101 cgatgaccgg ctggatgtga ccacctccgg cgagttcatc cccgccggca ctccggtgca
     8161 gattgtggaa gcccgcggca gccggattgt cgtgcaaaaa atcgggtgaa tgcgggcggg
     8221 cgcgattatt tcgcgccgcc cacttcaaac ggataatcgc acgggcgccc gcggaagcag
     8281 aagccaaggt tgtcgccctt gacgtcgtcg tactcgatcc cgcgcagggc caacgccacc
     8341 ttttcggcgg tcagcggcat ggttttcaac cgcacaccca ccgcattttc gatggcatcg
     8401 gcgacaatcg gcgcgcaggc gatcatcgtg tgctcgccca cgccgcgcgc gccaaagggg
     8461 ccgtcgggct gcgcggtttc caggatgatc ggcacaattt cctgcgggca atccaacgcg
     8521 gtcgggattt tgtagtcggt gaagtttgga ttcaacaacc ggcccgtggg cgcgtagcga
     8581 atgtcctcgt agagcgcggt ggccaagccc tgcaagacgc caccgaccac ctgccccttc
     8641 accagatcgg ggttgacagc tttgccggca tccacggcca acgccacctt gcggatggac
     8701 attttgccgg tgtccttgtc cacttccatg atgatgccgg cgcaaccgac agtgtagtgt
     8761 acgttcggat ggccgccttg gctggtttcc ggatcgccca tggcggaggt aaactctggc
     8821 atgaacatcc ccgtgccttg gatggggccg ccaatgaagt tgcccgcggg ccgcatgatg
     8881 ccgtcaatta cgaacgaacg cagcggcagg ctgaaggctt tgtcgcggcg gctcttgacg
     8941 cactcgtctt caagatacag atccgccacc ttgagttttt tggcgcgagc cacggtggcc
     9001 agaatcttgg ctttgacttc ttcggccgcg gcgcgcacgg cgttgccgca gctccatgtg
     9061 acgtgggaac cgacggtctg ccactcgtag gggttgcggt cggtgtccgg tgtttccacc
     9121 cggattttcg ccaccggcac cgtgagcact tccgccgcaa tctgggccat gacggtcagg
     9181 aatccctgac caatttccat gccggacacg ctcaagttca gcgagccatc ctcgttgaat
     9241 ttgagaaacg cggccgagga cgcgttcggc ggcatggccg gcgctttcca gaaacaggcc
     9301 acggctttgc cgatggccat attgcgcttt tggctgcgca ccgctttgcc ccactggatt
     9361 tccttggcca cgcggtcaat ggcgccgtgc aaatcgccgg cgttcatgtg cgcgccgtag
     9421 gccagcgtgt cgccttcgcg aatggcgttg agtttgcgga aggccaccgg gtccatgccg
     9481 atggctttgg ccacgcgggt catgtgcgac tcgaggccaa agtggaattc ggaatagccg
     9541 aagccgcgat aggctccgca cggtggcatg ttggtgtaca cgcaaaccga gtcaatcttg
     9601 acgttgggca cgcggtaagg ccccgtggcg gacaggccaa cggcattcac cacgttggcg
     9661 ccgtattccg ccgacgcgcc cgcatcccag tgcagggtgt gctccagcgc agtgattcga
     9721 ccatcctttt tcacacccag cttgatgcgg gcaatcacgc cctgccgctg cgaggtgttc
     9781 atgaactcct gctcgcgcgt ccagttcagc ttcactgggc ggcccttcat cacaatggcc
     9841 agcaccgcgg cgatgatttc catggagacg cccgccttgc cgccaaagcc gccgcctaca
     9901 tacggcgtca caatgcgaat atccttgtgc gacaggccga acggcttcag ggcaatggcc
     9961 atgacgttgc gctgcgcaaa aggcgattgc gatgaagccc acagcgtgag ccggcccgac
    10021 aagtcgaaca ggccggtcac cgcgtgcggt tcaatggggc aatgggcgta gcgcggcacg
    10081 gtgtaggtgt cttccaaaat gaagtccgcc tgcttgaagc ccttggccac atcgcccttg
    10141 cgggttttgc gccagtgggc aatgttcgtg cccgcccgcg ggaaaaacca cggtacgcgc
    10201 tggtaggaac ccaattcggg gtgcaagagc acggcgtctt ttttgagggc gtcgcccagg
    10261 ctcaaaatgg ccggcagagg ttcatattcc acctgcacca gcttggctgc gcggagggca
    10321 gcttccggcg tttccgccac caccgcggca atttgctcgc caacaaaccg cacccggtcc
    10381 tgcgcgaaaa tatgacgatc ctgcatatag agcccaaact tgtgcgggca atccttgccg
    10441 gtcaccacac gcaccacgcc gggcgccttc tcggcagccg acacatccac cttcttgagc
    10501 agggcgtggg ccttggtgct ttcgaccacc accgcatgca aaaggccggg gccgaaatcc
    10561 agatcatcgg tgtactgggc cgcgcccatc actttggcga aaccgtccac gcggacgggc
    10621 gatgcgccga caacttcggt tttactcatg accgggccct ccgcttggcg gacttgcgtc
    10681 cggttttccg ggcaacgtcc agcacggttt tcacgatggg ctcatagccg gtgcagcggc
    10741 acaggttgcc ggccagcgcc tcgcgcactt catgctccgt gggctgcggt ttttccgtca
    10801 gcaatgcgga agccgccaac accacgcccg acgtgcagaa tccgcactga aacgcgttat
    10861 tggccacaaa cgcctgctgc aacgccgtcg ggccctgctg ccccactcct tcgagagtgg
    10921 tgacggactg gccgtccact tcgatcgcca agatcaggca actgcgcacg accttgccat
    10981 cgagcagcac ggtgcacgcg ccgcaatcgc cgcgcccaca gccttccttg ggcgttttga
    11041 tgcctagctt gccacgcaaa acatccagca gggtttcgtt tggtgccacc gccacacggc
    11101 gggccgcgcc attcaagttg atggtgattt ctttcatgag cacgcttcct ccagaacatt
    11161 gatgccatag tccgcgccgc ggcccgccaa gcgggcggta gccagctcta ccgtgcgctc
    11221 cagcatgatg ccgatcatca gccgacgata cacctccgag ccgcggatat ccgtaatggg
    11281 cgccacctcc gcctcggcca acggtcgggc tttttgcaac aattcgacgg gaggattttt
    11341 ctgcccgcgc agcacttttt ccaccttggg gccgcggatg ggcgtggggg ccacggagcc
    11401 gtaggccacg cgccattcgc cacgggcatc cacccgtacc gccacgctgg cttgcgccaa
    11461 atcttcgcca cgatagcgtt tcagtttggc aaaagcaccg gcgtgtttgc ccaccggcaa
    11521 actgatcgcg acagccaaac cacccttggc caacgcggtg cggcgcgggc ccaagaacca
    11581 tttggtggca ggaatatttt tggcgccttg ccgagtggcc acgtgcacgg tggcttcata
    11641 taccagcagg gccggtccgc tgtcgcagca gggaaccgcg gagcaaatat taccgccgat
    11701 ggtggcgcgg ttgcgtaccc ccaccgaggc caccatgccg gcggcttcca ccaaaatcgg
    11761 cgcgtgcttc cgcaccagcg gcgactcaat gatctcggca aacgtagtat tggcgcccag
    11821 ccataggcgg ccatttttca ggcggatccc gcgtaattcg ggcaggccct tgatgtccac
    11881 cagaaacgcg gggcttactg cgccatcgcg cagccaagcc accagatcgg tgccgcccgc
    11941 cagcgggaca cgccccggct gggcggccag caggcgcagc gcggcggaca ggctgtccgg
    12001 cttggcataa tcaaattcac aggcaatact catgtttgtt ctccctcgga ttcgccaccc
    12061 tccggcaagt ttgtgccggc caaaaggtct tccaatttat cgtcaaccgg caggaaactg
    12121 ccgtcgcctt cgtggcgcag gcccaccaaa atggtcttga acgtgcgtaa caggatggac
    12181 acgtccagca aaaagcccat gttcttgatg taatacaaat cgaattgcag cttgcgcgcg
    12241 gcggcctcga agcttgccgc gtaggggaag cgcacctggg cccagccggt caggccgggc
    12301 tgcaccaagt ggcgctcgtc atagaacggg ataaagcgcg ccaacttttc cgtgaactcc
    12361 gggcgctcgg ggcgcggccc aaccaagctc atttcgccct tgaggatatt gaagagctgg
    12421 gggatttcat caatgcgcca tttgcgcagg tagtagccca ccttggtcac gcgggtgtcg
    12481 cgctggccgg cccagaccgc gccctgcgcc tcggcatcca tccgcatggt gcgcagcttg
    12541 tacagcacgt agggcttccc ccccagcccc agccgggttt gccggtaaac caccgcgccg
    12601 ggcgaggtca gtttgattaa aatccccgcc aacacaatga gcgggagccc aagcaacaac
    12661 ccaaacaccg aaaccacgat atcggttgcc cgtttgacct tgcggatctg aatcacggag
    12721 ctgttcagcg ccgcggtcat cagccattca tcgttgatgt gagccagcgg aatctcttgg
    12781 gccaactcct ccgacaacga gacatagtcc atgatctcga cgcccatgta ccgcagcggg
    12841 cgcagccgcg cgaggagttc acggctccgc atcaccgaca ccgaaagtag caacacggca
    12901 atctcgcgct ccgcgaccaa tcggtccaag tcgtccaagg tgcctagcac cggcattttt
    12961 ccggtgtatg cggaagccgg cgttgttccg cacaccacca ggcccgccac ccgatagaaa
    13021 gagaacggct ggtcttcaac caactgcagc agttcggcag cccggtcggg atggtcgcac
    13081 aacaccagcg cctgcttgag aaaaataccg cgccgcatca gggccaaaaa tacgcggcgc
    13141 aagaggtacg tcaggatcaa aatcagcacc gacgacagag cccaaatgcc gcggcccaag
    13201 accatctgtg ggcgggtgta caacaccaag ccagtaatgc cagccgcaat gccggtgaca
    13261 atcaagggca gaaaatcaaa atcgaaccgc cggctgcggg tgggcggctc atacatcccg
    13321 cccgcgtaaa acacgaggcc atagatgagc gccgaactga cgagcgagcc ccagttctcc
    13381 accatgtatg tgccgccatc ggcaaaacca aggcgcaaaa ataccgccgc gagttgggcc
    13441 aaaatgatga ccagccagtc gccaatcgtc agaaacaaat gccggctgga aacctgcacc
    13501 ccgcgatgaa aaatcaccac caagtcacat tctcctaatc atccatcttc aagaacttgc
    13561 ggcggggccg gcgccgggtg tcacaggcga cgtgtcctct tcgtagcgat agtcataacc
    13621 gtaatagtga tagtagctgg agtagccgta gttgcccatg cgggtcaaat ccaagttgtt
    13681 cagcacgcac cccagcacgt tggcgccgcg cgacaacagt tggcgcatgg aagcctgcac
    13741 cagtttgcgt gaggtccgcc cgccccacac caccaacagc gcgccatccg tttgtgcggc
    13801 gatgacggtg gattccgaga cagccaacgc cggcggggca tccaaaatca ccaagtcata
    13861 tttctccgat gcttccgcca agaattcccg catttcacga cgcatgacca attccgcggg
    13921 gttcgtcggg aaagttcccg tcccgaccaa gtccaggccg gcttgtgggg tgtgctgaac
    13981 cactttgtcc aacgtggttt ggccggccaa tatttcgctc aagccgcggc ccgcctgcaa
    14041 tccgaagaag cgatgcagac cgccgcggtg caagtcggca tccaccatca acacccggtg
    14101 gccggtctgc gcgaaactgg tgcccagatt cacgctggtg gtggttttgc cttccttggg
    14161 cacggatgaa atcaccgtca aggtgcggaa cgggcggccc gtcgggttca gcagcaaggc
    14221 cgaacggatg ttacgataag actcggcaaa tccactcgcc ggatccacgt tgcccagcca
    14281 gtacgacgag tcatcttcct gccgccaatg ggccgtgggc accaaaccaa ggaagttcgc
    14341 gcccaatgcc ttcccaattt cttcgggata ccggatggaa tcgtccatga attccagcga
    14401 aaagatcagc cccaacccga tggccaaacc gatcaaagcc gccagaaaga ggctttgcat
    14461 attgcgtggc ttcaacggac tgcctggcgc gagcgcccgc tccagaatct tgacggactc
    14521 cgtttggatg cccgccgaaa tatctatttc ctgcagccgg ttgaaaatca aatcgtacag
    14581 cgactgcaac cgcttgagat tgcgctgaat gccttcgtac tctttttgct tgcgatcaat
    14641 ctccagcgcc tgctcttccc actcctgctc cacctgctgc gcagcctttt ccttgatgct
    14701 caaggcttcg agttgggagt agtactgctt gagcgcaaat tgcacttcca ccttcaacgc
    14761 gtcttcgttg gcctgcagct tgcgctgggt ttcctgaatg aggggatgtt cgtccttata
    14821 tttcttgcgg taggccacca gttgcgcttc gagaatggag ttttcgcgct tcagcgtgct
    14881 ccattgtggc ggggccacca cgccgtgttc aatcaggctc tccgcactat ccacggccgg
    14941 gctgtcgccg gccggcaaac cgcccttgga cacctccacc gcgggcgccg gcaggccgta
    15001 ttccaagatg gccagcacga cttcgtccgg tgcctgattg agcagcggtt gctgcgcttc
    15061 cagcaacatg cgctgagtgc gatgttccgc cgcctgcttg gaaagctggg ccagcagatt
    15121 cgccgccacg ttgccgcgtt cctggatgcc aaccacgcta ttttcgcgca cgaacgccaa
    15181 caaattgcct tccatgctcg caatcttttc cgccagatcc tgggcttggc gcgacaggtt
    15241 atccaccgtg ttttgcgaac tgccgtcgta ttccaccgac ttgtattcca aataggcatc
    15301 cgcaatggca ttggcataat ccgcgcaaaa accgggctcc aagccggtca cgctgatggc
    15361 gatgatcgcg gtttgccaca gcgggtacac cgagagttcg cggaactttt ccccgaattc
    15421 ctgcggtggc attccgagct tggcgcgggc cgtttgcagc acatcccggc tgcgaatcac
    15481 gttgcattgt gtttgggcgt aatcgccacc aacggcttct gcttcgcgcc gttgcaggtt
    15541 ggccggcaaa acggccgaac gcgtcagcag gattttgcag gtggcctgat attccggcac
    15601 ttggcgcgcc atggtgatca gcatgatcac aatggccgtc atgaagcaca gcagcaacag
    15661 ccagaggcgc ttggtgatga tgtaaaaata tttgcggatg tcaaagccct tgggcgcgga
    15721 ctccgcgggt cccgtttcag ggggcggcgg ggtgttgccg gtggtcggtc gttggatcat
    15781 atcgcgcatg attacagccc taacaaccct ttctccggca caataatgac atcgccatcc
    15841 tgcaggggaa catcctcttc aaacgaacct tctttcaaga tatggcccac atccaccagc
    15901 atcgtttttt tgctcccatc cggcgccgtg cgctggattt gaactttgct ggggttggca
    15961 aactgggtca tgccgccctg gctcaaaatg acccgcgccg ccgtggcctg cggccccgaa
    16021 ctcaacgcca cggcccccct ttggttgact tcgcccagca gatacaccaa ctcgcgcact
    16081 tgattgcgaa ccgacggaat gaaaaatatg tcgcccttct gcacgggcag attcatggtc
    16141 atgtccgccg cgccgaagag ccggtccaga ttcagaggca ccaaggcata ttcaccactg
    16201 gcccgtggcc ggagaatacg cgcgccgctc cagtcggcaa actcgcccag cccgccaacc
    16261 atggtgacga ctttaatgat gtccaacccg gggacgtacg gatggaaccc tggagaaccc
    16321 acttccccca acgctaggaa ttcatcgcgc ccctcctcca gaccgcgcgc cggtacaatg
    16381 atcatgtcgc gcggacgcag atactgatcg tccgaaaaat ccagcgtggt caacattttt
    16441 tggacattca cttccaccgt ttgccgctcc atgctgccgc cgggggtcca ccggagaatg
    16501 cgcacccggt ctcccgcggc atcttcgcgg aggccgccag cccgggtaat ggcttccatc
    16561 agcgttaaca gcgagtcgcc acgaaacggg atgctgcccg gcgactgcac cgcgccagta
    16621 agcaacacat cgccttccac aaagttggcc acggaaatgg tgacgttggc ttccttgaag
    16681 taacgatcct ccagcacact ttcaaggcgg cgcgccgctt cgtcggcggt caactcggca
    16741 atcaccacgc gcccggcaaa tccgacatca atggaaccgt cgccggcgac agcataaacc
    16801 cgattcatgt cgggctgttc gcgaaccgaa atgttcaggc ggtccccggc catgatccgg
    16861 cggccgcccc cctccgcggc tcccgctttg ggcttcgttt cgccggcacc ataggccccc
    16921 ccggcgaatg cacacaggca cgccactaat ccccccatcg caagtccaga caacaggcca
    16981 gatttcatgc caaaaactta cccgacccga gagggcaaga caatgcccga tttcactaaa
    17041 agcgccgcca aagaaccgcg cggctaaaac gcgtaaatca gcgtgtacat cacgagatgc
    17101 ttctcgttgg cgccaccgtg atggaaattg gagttttccc acgtgtatac gtacgacaga
    17161 ttacgcgaca gtttgcggga tagctgccgg gtatgatcca gcccggtcgt aatcgtggtg
    17221 gttttttcag tttccggatg gggtactcca agcggtgtct ccaactcata gactgctgaa
    17281 aagctgaagt tcaacccata taccagcagg tcccgtagcg agaaatcata accgtaagtg
    17341 gtggactcgg tatcgctgtt cgacccaaag gtggcttccg gctcttgggt gaaggtgagc
    17401 gagtgctgcg cgcgcgggcc gaggagctga gtaagcttga tgttgtactg gaacgtcacc
    17461 tcgtcgtctt cgttatcttt attttgaccg ggcaacgcga agtcgggggt gctgttctcc
    17521 cacgtatctt cccatgtggc cgaaagtgac aggtggatgc tcttggacaa atcaaattca
    17581 tccgacacgg tgatggaatg cgttggctcc caggtcggct cctcccggcc agcggtctga
    17641 tccacttgtt catcttcata gctgaagccg aactgatagg tgatcttggg ccgccgtaaa
    17701 atatccacgg ggatgctgcc atcaatgccg aatgtctggg tgatttcagt ggtttcctgt
    17761 tcagattgca actgggtggt gaccacccgc tcccatgtgt aaaaaagact gccccagcgg
    17821 aacacatcca aaaagatgct ggccgtcgtg gtaatttctt cctgatcgcc cgcttgatat
    17881 tgctcaaaac ggtgcatttc gcggctatat tcctcggaaa actcaaggcg caatatccgg
    17941 tagttccaat tggcgaaggc gttggcgtcc aacgtcaaaa ccggatcacg gctggtcccg
    18001 cccggcacgt attggtcttc aatcccatcc acagaataat ccacactgac cattccgccc
    18061 agcgtcaggc gcgggtgtgc ggtttggaaa ttcacggcca cgttatacac ctcggtgtcc
    18121 aaatcgttgc ggcggaaata atcttcgtaa ccaatcattc ccgacacgtt gaaggtgatc
    18181 cggggccaca tggtcagcgt ttgcgactga atggacaggc tcggcatcag atagaagtcg
    18241 tccttgatca tgccgggctt ttcgtcatcc ggatagatgt catccacgtt gctgtcgtag
    18301 gccaaactta acgtgccgac caaggtcagg tccagattcc ccacgcgcat ggtttgcgcc
    18361 agccccgcac ccggccagaa aaacaacgcc gccagagcca aaacagcacg aagaccccat
    18421 cctgcccaac gccggtgacc acagggcagt gtggtcacgg gatacccccc tgccttggat
    18481 tgggccgcct cgcgcatgag cacatccacc cgccgcagac ccggcgctca gccatcagcc
    18541 tgcccggcgg gtgaccgggc gtctaccggt cgctcttgtt ggcgttatat tcgcgaactg
    18601 cggcggccgt ggagcccgag aaatcgcccg gctcgcccca ctgcaccact ttgccatttt
    18661 ggaaatacac ccagtaggtc ttgggcgtcc aagtgaacgc cggcggcagg tattcccaga
    18721 cctcggcgta ctcctgatcc ttatagattt tcgaggcgcg aaccgtgaac ggcttgccga
    18781 tggcttcccg caccgcttcg ggctccatgc ccaactgcaa cttgctgaca tcaacattcc
    18841 aggacgcgca agcgctcaaa ctgagcgcaa tgacgagccc caccagtatc ccacttgctg
    18901 ttcgcatcat tggtctctcc ctgttccgga ggttctgcct tacccaaaca atttcgtaac
    18961 tttgtgccat gaaagccctg ccatggcaag caaaagacag aagtatttcc tcttccggga
    19021 gcgcgtccgg ccgccgggtc tagcgcttca acgccttgat cagctccggg atcacctgga
    19081 acaagtcgcc cacaatgccg atatccgcca cgccgaagat tggcgcatcg gggtccttgt
    19141 tgatggcgat gatcgtatcg ctgctgttca tgccgaccaa gtgttgaatc tggccggaca
    19201 ccccgcaggc gatataaatc ttgggccgga cggttttgcc ggtttgcccc acctgatgga
    19261 aactggaaat ccagccggca tccaccgcgg cgcggctggc gccgatggcg gcgcccccga
    19321 gggcctccgc caactccgcc aacagcttga agttttccgg ggaacccagc ccgcgcccgc
    19381 cggtcacgat gaactcggcg ccggccacat tcacctcgtt ttccatggca ttgacgactt
    19441 ccagcacctt caccaccggg tcttttaacg tcaacggctc cacaatcagc tccccggtcc
    19501 gcccggccac tggcgtcggc tgcttgaaca ccttgggccg cacggtggcc atctgcggac
    19561 ggtgatccgg gcagataatg cacgccatga tgttgccgcc gaatgccggg cgggtttgca
    19621 gcagcaagcg cttttccaca tccacatcca gctcggtgca atccgccgtc aatccggcgc
    19681 tgacctgaat cgccacgcgg gcaatgaatc cgcgcccgat gctcgtggcc cccgccagca
    19741 caatttccgg cttatatttc ttgatcaggg cgctcatggc ctcggagtac ggctccgccg
    19801 tataggccgc caacgcgggg tggtccacca catagacctt gtccgcgcca tgctcaaaca
    19861 gtgcggacgc gcccgcctga atctggtgac ctagcagcac tgcgctcaac tccacaccca
    19921 gcttgtccgc caagcgccga ccttcgccca gcaactcaaa cgccacttcc tgaatccggc
    19981 ccgcgcgctg ttcggcaaac acccacagcc cgcggtacgc cgcctgctcc tcggcggaca
    20041 cctgccgctc agcaacctcg atggccagcg cccccaccgg gcacacctcc acgcacgcgc
    20101 cgcacgccgt gcaggcctcg ttcacttcca ccttgccgtc ttccgcccgc tgggtcagcg
    20161 cgcagaacgg acacacggca acgcacgcgc cgcaaccggt acacaaatcc aaatcaatct
    20221 tcagggccat ggttgccgtc tcctaaatca gtttgcgttc gcggagcaca tccgccaggc
    20281 gctgcgccat cacggcgggc tcttcaccct gccacttcac gccgccggtt ttgggcggcg
    20341 gcgaaaaaat cttcaccacg cgcgtcgggg aggcattcag ccccagcttg gccgggtccg
    20401 cgcccacccc ttccgcggtc agcggcacga tgctcaattt cttggccgcc attttgccgc
    20461 gcagcgaagc catgcgcggc acattggcct ccttgagcac ggtcagcacg cagggcaggg
    20521 ccacttccag tttttcgtag ccgtcctcgt aggcgcgctc cacctgcgcc acgccattcg
    20581 tcacttcgat tttgcgcgcg taggcgatgc acggcaggtc cagccaatcg gccacgcccg
    20641 gccccacctg cgcggtatcg ccgtcaatgg cctgcttgcc gaacaggatc aggtcgggct
    20701 tttccatctg gcggatggcc atgctcaacg cgtagctggt ggcccacgtg tcggaaccgg
    20761 cgaacgcccg gtcgctcagc agcaccacgg catccgcgcc gcgcccgacg gcctccttga
    20821 gcacctcggc ggcctgcggc gggcccatcg tcagcacggt caccttggtg ccagcgtgct
    20881 gctccttcaa ctgcagcgcc atttccagcg cgttttcgtc gaagggattc atgatggact
    20941 ccaccccctc gcgcaccagc gtattggttt cggggttgat ccggacctgc gtggtgttgg
    21001 ggacctgctt gacgcaaacc acaatgtgca ttgacctctc ctcccggcgg gaaccgccgt
    21061 gccttggcgc cgcgaaccgc ggcctacttc ttggccaccg cgccgtattc cttgttcagc
    21121 gccagcccga tgatgtcgcg ctggatttgg ttggtgcctt cgtagatttg caaaatcttg
    21181 gcgtcgcgca tcattttctc gaccggatat tccttcatgt agccatagcc gccgaacacc
    21241 tgcacggcat ccgtggccac ctgcatggcg atatccgagg catacatctt gcacatggcc
    21301 gagaacttgg agatgtcctt cgcgccgcta tcaatggttt tgaccgtcgc gtaggtcagc
    21361 gcgcgggcgg tttcgacctt ggtggccatt tccgccagca tccagctcaa cccctggttg
    21421 gcaatgatgg gccggccaaa ctgctcgcgc accttggcat aagccacggc ctcgtccaac
    21481 gccccctgcg ccaagccgat ggcctgcgcg gcgatgcccg gccgcgacac gtcaaacgtc
    21541 ttcatcgccc aaatgaaacc ggtgccttcg cgcccaatca ggttttccgc cggcacttcg
    21601 caatcttcaa aaatcagctc gcgcgtcgcg ctgcagcgga tgcccagctt gtcttccttt
    21661 ttgccaaacg agaaccccgg cgtgcctttt tccacaataa agcagctcac gccgcgcgcc
    21721 ccgcggctct tgtccgtcac cgcaaaaatc gtataaatgt cggcctcgcc gccgttggta
    21781 atccactgct tggtgccgtt gaggatgtat ttgtcgccct tcttgaccgc cgtggtgcgg
    21841 atggaacccg cgtcggaccc cgcgttgggc tccgtcagac cgtatgccgc ccacttctcg
    21901 ccgctggcca gcggcggcag gtactttttc ttctgctcat cgctggcgcc cagcaagatc
    21961 gggtccgcgc ccaacccgtt ggcggcaaac gacaccgaca cgccgatgca aatccggctc
    22021 agctcctcca ccgccacaat atgcggcatg ctgtcaccct cgaacgtccc gccatactcc
    22081 gtcgggatat acagccccat caaatccatc ttccccagtt cccgcagcaa ctccgtggga
    22141 aacacccctt tttcatccaa ctccgcccgc accggcttga cccgggcctc cgcaaactca
    22201 cgcatcatgc cccggatttc caacgccatt tccgacaagc cgtaattcag ttctgccatt
    22261 tccagactcc ttatgccgcg tgccgccacg ttgacgaaca cggcagcgga ctatgccaga
    22321 gccccccccg ccgatgcaat ccaaagatgc cgtcgggctt gtgcgcatgc caccgtcacc
    22381 gatagtccga ggaaggggtc ggtgccggtt tgggtgcgga tgctgccgaa ggcggcgtag
    22441 gtgtaggttt ggacgggctc gccgctctcg tccgtcaaca cggcgatgga accgaggcca
    22501 tcggcatgga agacctgccg gttgcgggct tccccgttaa tgaaggcaat ccgctcgacc
    22561 ggttgatcca gccccggcgc attcacccac gccc
//
LOCUS       DAOPNP010000030        22310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2907_length_22310_cov_8.476432, whole genome shotgun sequence.
ACCESSION   DAOPNP010000030 DAOPNP010000000
VERSION     DAOPNP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22310
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2907_length_22310_cov_8.476432"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..909)
                     /locus_tag="PKI64_04160"
     CDS             complement(163..909)
                     /locus_tag="PKI64_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE00239.1"
                     /translation="MSGHSKWSTIKHKKGAADARRGKIFSKLAKELTVSAKASGGDPA
                     SNITLRTILAKCKAVNMPADNIERAIKKGTGELQGAALEEIMFETYAPGGVGVIILCL
                     SDNRNRSVAEVRHVLQRNNSTLAGSNQVLRNFQRRGQILVDAEGVDEDQLIEIALDAG
                     ADDVTHEGTEFEVLTAPNVYNEVSEKITAAGYKVSEDSGINFVPTVWTPVTDKAQATA
                     ILKMIEAIEELEDVQNVYMNGDIADEAMPD"
     gene            complement(981..3002)
                     /locus_tag="PKI64_04165"
     CDS             complement(981..3002)
                     /locus_tag="PKI64_04165"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE00240.1"
                     /translation="MKDIPVADVRNFVLVGHTGSGKTSLVDALLYKLGVNDRLGNPGD
                     GTSMADWTDEEKNRKISIWAKPFDAVWRPKGANPLRLVMLDTPGYADFYGQMVAATQV
                     ADAALLLVDATAGVQVGTVRAWKRCEALGLPRAIAITGLDKENADFNGTVAALQDLWG
                     VRCEVVTFPTADKKGVVDVLSGQSPEAEELRSKLTDVAAESSDELIEKVLNGETLSAD
                     ELAQGLRSSVVQTKFIPIFAVMARQSLGLDELMQGFGRLMPSPADRPVKDESGAEVNA
                     AADAPLVAQVWRSINDPFVGQLTLARIWGGTLKENSEIFNATKEQKERVGTIYFTNGK
                     KNDPTPEAQAGDIVALAKLKSTGLNDTLCAVGKPAKMAPIVFPNPVASFAVMPKNQGD
                     EDKLGQGLSRIADDDPTIHVQRNDETRELIITGMGDVQIDVAMERMKVRSKVEVTLST
                     PKVAYRETVTTKGEGHYKHKKQSGGRGQYGEVYLRIEPLRPGVEHWFVDGIVGTSIPR
                     NFLPAIEKGLVEGMTRGAVAGYPVVNTMVTVYDGSSHDVDSSEIAFKIAARTAFKDGM
                     AKAKPVLLEPIMNIRVTIPAEFMGDCTGDLNHKRGRIMGMDAEDGMQVIVAEVPQAEV
                     FKYSSELRSMTGGRGSFEISFNRYDIVPSNVAQKVIAEAAKNKQAEEEE"
     gene            complement(3160..3453)
                     /locus_tag="PKI64_04170"
     CDS             complement(3160..3453)
                     /locus_tag="PKI64_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE00241.1"
                     /translation="MKVEWAPLAIDRVAEIAAYIAEDNPSAAEKWIRNIFVRVGQLRQ
                     FPRSGRAIPETTRKDIRELIWGNYRIIYRITARRVAILTVRHTKTILPLAELD"
     gene            complement(3450..3731)
                     /locus_tag="PKI64_04175"
     CDS             complement(3450..3731)
                     /locus_tag="PKI64_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE00242.1"
                     /translation="MPRLNLAEDIKPLSEFRANVTTYIRQTRQNRRPLVITHNGKSAA
                     VLLGVAEYERLLRKMEVLEDIRLAEDQLARGAGVAHDAAQKQVLAKVRG"
     gene            complement(3834..4955)
                     /locus_tag="PKI64_04180"
     CDS             complement(3834..4955)
                     /locus_tag="PKI64_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOE00243.1"
                     /translation="MMRVLMVVQTAMSLGMGEQVFLTGSTDELGRWNVATVPMTRTDD
                     CRWEATLWLRDSTPVEFKVTRGSWETEEVDAQGRLMANHVFTPADGAVVEIKVAAWKD
                     ERPAPATPRITGDYRHLPQVHSQFLAHDRDVVVWFPPGYDTQPTKRYPVLYMHDGRQV
                     FDPTTSTWGKDWQVDEIAQHMILNGELEPFMVVAVDCTEARREEYAPAHKGDDYLRFL
                     LEELKPMVDATWRTDPHRTAIAGASMGGLISFYAAWKRPDVYFGAACLSSAFLERYDR
                     ECFALVAAAEGNWPDLKLFLSCGGAAGLEAELLPGTLRMADTLRRGGYPEKNLNVRIE
                     SWAEHNEEAWSRMTPHWLRFLFGRPQLAEPDPDARHKAL"
     gene            5054..5890
                     /gene="lpxA"
                     /locus_tag="PKI64_04185"
     CDS             5054..5890
                     /gene="lpxA"
                     /locus_tag="PKI64_04185"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOE00244.1"
                     /translation="MLPAIIQELLSMIHPTAIIEPGAQLGVNVSLGPYAHVAATARLG
                     DGCVLDSHAVVKDYTTIGARCRLHTGAVIGGEPQDLSFKNEPSYVVTGDDCIFREGVT
                     VNRGTKPGTTTRIGNHVFMMANSHAAHNCEVGDHVILANGVLLAGYVTVGERAFLGGG
                     VGVHQFCHIGRLAMVGAISVITKDLLPFCVAANCANSMVFGLNIIGLRRAGFTPEQRA
                     QVKQAFHVLYRAGLNTTQARDQLSAATDNPFAQEYAEFLSRAKRGICRFSHTVAADEA
                     DA"
     gene            5883..6764
                     /locus_tag="PKI64_04190"
     CDS             5883..6764
                     /locus_tag="PKI64_04190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE00245.1"
                     /translation="MPDAPVIRTAIGGFPDYELVDSGRRCKLERFGPVLVIRGEPKAW
                     WEPVLPPAEWDRAQAIHDEDHGWDLRPGCPREWRMRYGNLTFLAKISDTSKHLGIFPE
                     QAPHWRAIQQAAAPGARLLNLFGYTGAATLVAADAGWQATHVDASKPAIAWGRQNQTL
                     SGMAEKPIRWLVEDAMKYVRREIKRGSRYDGILLDPPAFGRGPDGNVWKVERQLPELL
                     DLSRRVMTERPRLLLLTTYNIEASALMLRNLLADLMQPFGGHIEVGELALAHSAAPDR
                     LLPLSIYARWTPHPPPP"
     gene            6775..7728
                     /gene="thiL"
                     /locus_tag="PKI64_04195"
     CDS             6775..7728
                     /gene="thiL"
                     /locus_tag="PKI64_04195"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOE00246.1"
                     /translation="MPTLQTIGENALLRRILPRLPQRRDTIVGPGDDCAVVRMPGARH
                     DWLFTSDPVIEQRHFLPDTPARLIGRKAIARAVSDIAAMGGEPRWALIDLVAPAATPV
                     ARIRQLYAGMIAAAKKWNLSILGGDTAEGDTLELHIFGVGLIPRGRAVLRAGARPGDR
                     LYVTGALGGAYLPGSRHHLLFEPRLAAGQFLARTGFATAMMDLSDGLAADLPRLLEAS
                     RCGVQLNAAAIPITRAARRTAHPLQHAMGDGEDFELLFTVPAAKQARFATAWAKQFPR
                     LRATCIGEILANPRRRVIRLPDGRLQPLRAGGYQHFLPLQG"
     gene            7731..8846
                     /locus_tag="PKI64_04200"
     CDS             7731..8846
                     /locus_tag="PKI64_04200"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOE00247.1"
                     /translation="MPPVAYPHELESYWLNLAQTETRGVALRDTLQRLRPDIKRLADR
                     FTTARPATDTLPDYLRDTRDCAAYAVFFAPQTHARLTHILAELPPFPPTPQPLRVLDL
                     GCGTGAASFALLDHLGPRPVLLHAHDRSRAALRCLHECFSTLRASRWPAAMLRTQAAT
                     LEETAADGPFDVILLHYVLNELTPALRRSLLARAREWLAPLGRLIICEPLLREAGDYM
                     RELRTFALTDLGLHLLAPCPHEQTCPLAEPCHDVRTWKVPQSLQIVNNLLPRDLRHLA
                     YALLVLSPAPSPAPDGVIRARLVGSPTFAKGQTLCPACCSNGAIHRLQLLTRDFDTAG
                     RKNLRHLERGQILRLASLSPLPDSSLSRARPAPAEEE"
     gene            8910..9908
                     /locus_tag="PKI64_04205"
     CDS             8910..9908
                     /locus_tag="PKI64_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOE00248.1"
                     /translation="MEATQHMPLLLQPIYKEYIWGGDRLIHEFRRRLNPGIYAESWEI
                     ADHPDGRTRLINGPDAGATLSTAIARRGAELLGRGRTSATFPLLVKIIDARETLSVQV
                     HPDDDSAARFGGEAKSEMWYVLSATPDAHIYSGFKPGVTPADFAQARAAGTIPALLQR
                     FPARPGMVFHTPGGRIHAIGAGCLLLEVQQDANTTYRLYDWDRFDKNGQPRPLHIEQA
                     LQVINWDLTGSPVAPIQPEEARSPGHSARLLLSAPHFHVEEWTITGPARATHDGNSFL
                     ILFATHGDTRIKTNGQPAMIIPHGTTCLLPAILDTFTLQPAMPAGQPSRLLVARQP"
     gene            10062..10259
                     /gene="rpsU"
                     /locus_tag="PKI64_04210"
     CDS             10062..10259
                     /gene="rpsU"
                     /locus_tag="PKI64_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOE00249.1"
                     /translation="MTEVKIKKGEPVEKALRRLKKKLDREGIMRDIRAKRHFEKPSEK
                     RRRKAARARINARRATWAASL"
     gene            10338..11555
                     /locus_tag="PKI64_04215"
     CDS             10338..11555
                     /locus_tag="PKI64_04215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOE00250.1"
                     /translation="MKILVINAGSSSIKYQLMDLADAHRYAVLASGLVEKIGEATGRV
                     THTIFTDGQEQRFVEENPLKNHHEGMSRVVALLTDPQRGVIRDAAEIEGVGHRVLSCG
                     EVYSDTVRITPKVKATIREFFPLGPLHNPPNLMGIEVAEEFFPRAPQVAVFDTAFHQT
                     MPPKAFLYAVPYEWYTRHRLRRYGFHGTSHKFITQATAEFIGKPLAKTNLISIHLGNG
                     CSMAAVRHGKCVDTSMGVTPLEGLIMGTRSGDMDPAALEYIMGLTGMDIHQMVNALNK
                     QSGLKGICGVNDMREVVEKAAGGDAQAETALQMFCYRIQKYIGAYLAVVPHVDAIVFT
                     AGIGQNSAPVRERICTGLGHLGIRLDLAKNAARANGPRAIHQAGAPLQILVLPTNEEL
                     QIALETKQNITGA"
     gene            complement(11586..14747)
                     /locus_tag="PKI64_04220"
     CDS             complement(11586..14747)
                     /locus_tag="PKI64_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOE00251.1"
                     /translation="MARAHNFFHTVENFFPQCGKILAVALGAALLGGALVGCATVKAN
                     RLAVPAEKRWVVTASGAAEGHEAELAVDGRADTWWHCPGGKEAWLQVDMARAAMVCGF
                     SLQWGEPYAAAYALQTSLDGVHWMLSYETKTSDGGWDQVCFEPVLARHVRLLLAPELQ
                     GAALVALEIKGLADQPQAWVDGQPDPKAAALLDGREETAWVSTNPAAVVELDLRAARP
                     VGSVRVDWGTNGFAQQLTVEVSTNRTEWQRLGQLQPQVGDFDVLMAETALPAQYVRLS
                     FADGTGDDGCFALTGIALRGDEGTARPWARYEVAAAHAPEGIYPDLFRKRQTYWSVAA
                     GPAATDAEALLDEWGVFAPRPRGPTLTPLLLMDGQVLSAQQAERVEHRLGGEGAPLPE
                     VTWHMASGLKLRIQAQARTNAPAAMAWTMYELVNDSLMTQTGRWCWVVRPVRLALLGT
                     HGGLAPIYKIRVMPSESGWQEMWVNDEPLFAVPATNMPFGAVAFAQGDVAESLLRGEL
                     PRARTASDAEGLASGLWWRTFSLAPGGRMRWVVAGNALPPGVARPRRFPWPKVNGSPS
                     KVADLFSREWVDATWSWRDATGHYQPRIARPDAIECLHAQVGWLLGVRGVDGESTETI
                     ALRVAALLRAGQAKAALPWIEMVAAAQQDDGWVPVWFMADGTPAPRLRMGQYAGQGQL
                     AFMALEYYRFTQDAAFLQAMFPRLQNALAYLQRLRADQEKTDWLLPEDQRFLVDGLLP
                     ASGPRPGSAKPQHRYADQIWALLGWKETRTAAALLGAEAEATWADEQYRLLKAQVRRS
                     LRARMDLMTGMWVPGAAEDEQLDIQTVALLFWPGAETDLVEPHELQTSLDMFYADFLQ
                     RRQPGWSGVMANDEGALLGPLATMGRTDYAREVLYSLLDRRQPRGWQSWPDISTSDPR
                     QMGHVTAQPDIRAAALYFLGVRGLAARETDKQLDLFAGAPAEWLQHGEGFRVFGMPTA
                     FGPLDLAGRWEQKEFTVEIGGGAQPPAGYRLWWPRNMRPIRVLADGRDVTDFDGTGVN
                     LPPKFKGWVEVLFPQPAPWPRDP"
     gene            complement(14756..16075)
                     /gene="gltX"
                     /locus_tag="PKI64_04225"
     CDS             complement(14756..16075)
                     /gene="gltX"
                     /locus_tag="PKI64_04225"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOE00252.1"
                     /translation="MTIRTRFAPSPTGNVHIGNIRAAIYNWLFSRHAGGEFLLRIEDT
                     DLERSTPAAVQTVLDSLAWLGLTYDGAPLYQSTQRARHLEVAEMLLTKGAAYKSDKGG
                     TGKGEAIVFKMPGTDISFRDLVKGEITKKAEDLKDFVIVRSDGSPVFHLGNVVDDITM
                     GITHVIRGDDHVENTFKHVAMYAAIGAPMPQFAHLPMIVNAQGKPYSKRDGAAYVGEF
                     QDKGFLPEALFNSLALLGWSPGDDREVLTRDEMIAAFTLDRVRQAPSQLDMTKLLWMN
                     GQHLKRRGVEDLVAGCRAAMQRQGLWREDIDPAYFQAVIMCMGERIRLYSDLGEQAAF
                     FFTEEYPYDEKSVRKRLLKEGALAILREELEVLVALPEPFTAEATDAALHSLAEQTGR
                     QMGDLVHPVRVAVSGTGVGPGLFEMLAVLGKSRVVARIRRTLALYGG"
     gene            complement(16146..18122)
                     /locus_tag="PKI64_04230"
     CDS             complement(16146..18122)
                     /locus_tag="PKI64_04230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOE00253.1"
                     /translation="MSCDFAQHLNEAQLRAVTAPDGAVLVIAAAGTGKTRTLTHRVAW
                     LALERGVEPRRMLLLTFTNRAAREMLERAMQLIGQDVGGLWGGTFHHAANRMLRRHAT
                     LLGYGADYSILDEDDSTRLLKACINELGLRDKKFPKAEVLGSVFSLAANRGADVAETV
                     ADRFGAHPVDLTAILKVHTLYEQRKRALNAMDFDDLLVNGLKLLQEHESVAARYQERM
                     QHVLVDEYQDTNKLQNDFVDLLASGHGNLLAVGDDFQSIYSWRGAEVQNILSFERRHP
                     EAQVIKLEDNYRSTPGILDVANRVIAGNPEQYQKVLRATRPAEVKPVYVKLVDGGHQA
                     RYIVGKVQQLLDSGAAQPSEIVVLYRSHYHSMELQLELTRARLDYYLMSGVRFFEQAH
                     IKDVCSLPRLAVNPADSLAFMRLMEMLPKVGPKKAQALWRDLGQHVNLRDAAKRAQLT
                     KLLPKDAQPLWAEVDAKIFAPGEGEGDVLDRPAELIFRLVEAFYRDYAEETFENAEQR
                     LEDVEELMTFAAKYDDTAAFLSEMALLTNLDAEQNLHAGPPAQTIRLSTVHQAKGLEW
                     KAVFVLWMVDGMFPAARSLEEAANEAEERRLFYVAVTRAKDYLWLCQPRMRKTREGGV
                     MLCPPSRFLAEIDPGLLQEEKAGGMGAVPRWSRW"
     gene            complement(18136..19455)
                     /locus_tag="PKI64_04235"
     CDS             complement(18136..19455)
                     /locus_tag="PKI64_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOE00254.1"
                     /translation="MTLQDRVNKIRKFTSRALVALPGDERGGSLPARIIRVGHLLFRG
                     YMDDDLTIHASSLTFVTLTSLVPILAVGFALIKGFGMGQFDAERLAEFAWFTEMPDQW
                     QQFVHTILGIVNTTNFAALGWIGLAFFVLTAVLLLANVEKSYNRVWGVDKNRSLLRQI
                     ANYTSVLVLMPLLIGLVGALKARIAFNQQILGVDANAWVQNVAAFGIMWLAIGFLYVF
                     VPNTRVRLRPAVFSSLLTAVIFLGWIRMFTVLQVGVTNYNKIYGAFAAVPVFMLWLYV
                     TWVILLLGAEFTFALQNSDTYRLESAADTASMRARILVALMILRQAGRAMDGGKGFFD
                     VAEFARQTKAPIRLINTVVAMLARRGYLTQVASRDASYVLIRTPDSIRLHELVNKLMR
                     EGGSHAELEASLTVDTPLKETLAKIEQGAQQGFGNLTLADLVTASPS"
     gene            complement(19537..20646)
                     /gene="mnmA"
                     /locus_tag="PKI64_04240"
     CDS             complement(19537..20646)
                     /gene="mnmA"
                     /locus_tag="PKI64_04240"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE00255.1"
                     /translation="MKTNGRSVAVGLSGGVDSCMAAWLLKRDGWWVTGLTMSMWDGSV
                     AALDAGFAGCFGPGEAKHLAAAKANAERLGIEHQVVRLAAEYKETVLGYFRKEYLAGR
                     TPNPCVRCNQQMKFGFLLSRAKELGVEFDYFATGHYARVRRDETSGRWLLRRGCDTTK
                     DQSYFLSRLQQDQLAKLLFPLGEWRKEDVKKLAAEQGWADVAAQTESQDFVESKDYGV
                     LFRPDDAAPGDFVTRDGRVLGRHRGILHYTIGQRKGLALGGGGTPWYVLEIDAARNRV
                     VVGPQSELFHNVLIAGEINWLALAGPPEQPFRCAVQIRQQHKAAPATAHATAVGQIRV
                     EFDEPQLSITPGQVAVLYDGDNVLASGIIEKLPQI"
     gene            complement(20863..21471)
                     /locus_tag="PKI64_04245"
     CDS             complement(20863..21471)
                     /locus_tag="PKI64_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00256.1"
                     /translation="MNIQIEELSAERLRESADLLVDVFPSLEPLTEALHLSRDFAATY
                     VRTICDQCFEDHLAITALDTDTDRLAGIALSLDDERYRQTLRVVPAEYRLALGQMDEF
                     FEELSRPLAGFPGKGTNVFYLAVARPYWRAGISMALLRRLENLLRDKQYEYLVSEATN
                     IKSAGAFRKQGYIEMNRLAYATRPLFAGLPGDCTLFMKRLAC"
     gene            complement(21551..21727)
                     /locus_tag="PKI64_04250"
     CDS             complement(21551..21727)
                     /locus_tag="PKI64_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00257.1"
                     /translation="MNRIVQRAADQAWHFAGLLLYAPAGTTRFLRRAALITILEADVT
                     LAAAASPTASDLAV"
     gene            complement(22117..22191)
                     /locus_tag="PKI64_04255"
     tRNA            complement(22117..22191)
                     /locus_tag="PKI64_04255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22157..22159),aa:Gly,seq:gcc)
BASE COUNT         4138 a         7617 c         6343 g         4212 t
ORIGIN      
        1 cattgcatcc caatgtgtta tggcttctta gattggaagt gtcgaactca ggaaaaactc
       61 tataaaaccg gccatggccg ctgccaatcc cgaaaatacg ttttaaaaaa agcgccaaaa
      121 tccgggcgga tcctggcgcg cgagcgggac tgggggcggc atttagtcgg gcatggcttc
      181 gtcggcgatg tcgccgttca tgtagacgtt ttggacgtct tcaagctcct cgatggcctc
      241 gatcatcttg aggatggcgg tggcctgcgc tttatccgtc accggggtcc acaccgtggg
      301 cacaaagttg atgccggaat cctcggagac tttgtagccg gcggcggtga ttttttcgga
      361 gacttcgtta tagacgttgg gcgcggtcag gacttcaaat tccgtgcctt cgtgggtgac
      421 atcgtccgcg cctgcatcga gcgcgatctc gatgagttga tcctcatcca caccctcggc
      481 atccacgagg atttggccgc ggcgctggaa gttgcgtagc acctgattgg agccggccag
      541 cgtggagttg ttgcgctgca aaacgtggcg gacttcagcc accgagcggt tgcggttgtc
      601 ggacaggcag agaataatca cgccgacgcc gccgggggcg taggtctcaa acatgatttc
      661 ttcgagcgcg gcgccttgca attcgcccgt gccctttttg atggcccgct caatattgtc
      721 cgccggcatg ttcacggcct tgcacttggc caggatggtg cgcagggtga tgttggaggc
      781 tggatcgccg ccgctggctt tggcggaaac cgtcaattcc ttggccagct tgctgaagat
      841 cttaccgcgc cgcgcatcgg ccgcgccctt cttatgcttg atggttgacc acttgctatg
      901 tccactcata ccttgtcatg tccttcctgc cgcagggacg acaccttgcg tgcgccaccc
      961 gccggccgcc gggggaatga ttattcctct tcttcggctt gcttgttctt cgccgcttcg
     1021 gcaatgactt tttgcgccac gttggagggc acaatgtcgt agcggttgaa gctgatttcg
     1081 aacgagccgc gcccgccggt catggaacgc agttccgagg agtatttgaa cacctcggcc
     1141 tgcggcactt cggcaacgat gacctgcatg ccgtcttcgg catccatgcc catgatgcgg
     1201 ccgcgcttgt ggttcaggtc gccggtgcaa tcgcccatga actccgccgg aatggtcacg
     1261 cggatgttca tgatgggctc caggagcacg ggcttggcct tggccatgcc gtccttgaac
     1321 gcggtgcggg cggcgatttt gaaggcgatt tcggagctgt ccacatcgtg ggagctaccg
     1381 tcatagaccg tgaccatggt attgaccacg ggatagccgg ccaccgcgcc gcgggtcatg
     1441 ccttccacca agcccttttc gattgccggg aggaaattgc gcgggatgct ggtgccgacg
     1501 atgccgtcca cgaaccaatg ctccacgccg gggcgcagcg gctcaatgcg cagatagact
     1561 tcgccatatt ggccgcggcc gccggattgc tttttgtgtt tgtagtggcc ttcgcccttg
     1621 gtggtgacgg tttcgcgata ggccaccttc ggcgtgctga gggtcacttc caccttgctg
     1681 cgcaccttca tgcgctccat ggccacgtca atttgcacgt cgcccatgcc ggtgatgatc
     1741 agctcgcgtg tttcgtcgtt gcgctgtacg tggatggtcg gatcgtcatc ggcaatgcgg
     1801 ctcaagcctt ggccgagctt gtcttcatcg ccttggttct tgggcatgac ggcgaacgag
     1861 gccacgggat tcgggaacac aatcggcgcc atcttggcag gcttgccaac ggcgcacagc
     1921 gtatcgttga gcccggtgct tttgagcttg gccagcgcca cgatgtcgcc tgcctgcgct
     1981 tccggcgtgg ggtcgttctt cttgccgttg gtgaagtaaa tggtgcccac gcgctctttt
     2041 tgctccttcg tggcgttgaa gatttcggag ttttccttca aggtgccgcc ccagatgcgc
     2101 gccagcgtca actggccaac aaacgggtcg ttgatggagc gccagacctg cgccacgagc
     2161 ggcgcatccg ccgccgcgtt gacctcggcg ccgctttcgt ctttcaccgg gcggtcggcg
     2221 gggctgggca ttagccgccc aaagccctgc atcagttcat ccagccccag cgattgccgg
     2281 gccatcaccg cgaaaatcgg aatgaacttg gtttgcacca ccgaggaccg caacccctgc
     2341 gcgagttcat cggcggaaag cgtctcgccg ttgagcacct tttcaatcag ctcatcgctg
     2401 gactcggcgg cgacatccgt cagtttgctg cggagctctt cggcctccgg gctctggccg
     2461 gagagcacat ccacgacgcc cttcttgtcg gccgtgggga aggtcaccac ttcgcaacgt
     2521 acgccccaca ggtcttgcag cgcggccacc gtgccgttga aatcggcgtt ttccttgtcc
     2581 aaaccagtga tggcaatggc tcgcggcaag ccaagcgctt cgcaacgctt ccaagcgcgg
     2641 acggtgccca cctgcacacc ggccgtggca tccaccagga gcagtgcggc atcggccacc
     2701 tgcgtggcgg ccaccatctg cccgtaaaaa tcggcgtagc cgggggtgtc cagcatcacg
     2761 aggcgcagcg gattggcgcc tttgggccgc cataccgcgt caaacggctt ggcccagatg
     2821 gaaatcttgc ggtttttctc ttcgtcggtc caatcggcca tgctggtgcc gtcgccggga
     2881 ttgcccaacc ggtcgttcac gcccagctta taaagcaggg catccaccag cgacgttttg
     2941 ccgctgcccg tgtgacccac caaaacaaaa ttacgcacat cggcgaccgg gatatccttc
     3001 ataactcgtc tacagccttt ccttgcctac tcgttttgct cgcggccggg cgcaacggcc
     3061 cggccatgga aaagcgcacc gtacacaaaa agccccgcca accgcaaccg cgaaatgcac
     3121 ccccgcggca cgccaaatgc ccccacctgc cggtcgaatc taatctaatt cggccagcgg
     3181 gaggatagtc ttcgtgtgcc ggacggtcag aatggccacc cggcgggccg tgatgcggta
     3241 aataatgcgg taattgcccc aaatcagttc gcggatgtct tttcgcgttg tttctggtat
     3301 tgcccgaccg ctccgaggaa attgccgcag ttgacccact cgcacaaaaa tgttccgaat
     3361 ccatttttcg gcggcgctcg ggttgtcttc cgcaatatag gccgcaatct cggccacccg
     3421 gtcaatggcc aatggggccc attccacttt catccgcgta ccttggcaag cacttgcttc
     3481 tgcgcggcgt catgtgcgac ccccgccccg cgcgcgagtt gatcctcggc gagacgaata
     3541 tcctccagca cttccatttt ccgcagcaac cgttcatatt ctgctacacc cagcagcacg
     3601 gcggccgatt tgccgttgtg ggtgatcacc aatgggcgcc ggttttggcg ggtctggcga
     3661 atgtatgtgg tcacattagc cctgaattcg gacaagggct tgatgtcttc agctaaattg
     3721 aggcggggca tggacaatct cctttgtact gaaattagta cattattttg tattattgtc
     3781 aagggccggc ccaaccccgg gggttgggtt ggcgcggtgg gggggcggga gctttatagc
     3841 gccttgtggc gggcgtcggg atcgggttcg gcaagctgcg gacggccgaa caaaaagcgc
     3901 agccagtgag gcgtcatgcg gctccaggct tcttcgttgt gctcagccca gctttcgatg
     3961 cgcacattca ggttcttttc gggatagccg ccacgccgca gagtatccgc catacgcaag
     4021 gtgccgggca gcagctccgc ctccaacccc gccgcgccgc cgcaggataa aaacagcttg
     4081 aggtccggcc agttcccttc ggcggcggcc accagcgcga agcattcgcg atcgtagcgt
     4141 tcaagaaagg ccgacgacag gcacgcggcg ccaaaataga catccggccg tttccaagcg
     4201 gcatagaacg aaatcagccc gcccatgctg gcgccagcga tggcggtacg gtgcggatcc
     4261 gtgcgccaag tggcgtccac catgggcttg agttcttcca gcaggaagcg caggtagtca
     4321 tcgcccttgt gggcgggggc gtactcctcg cgccgggcct cggtgcaatc cacggccacg
     4381 accatgaaag gttcaagctc gccgttcagg atcatgtgct gggcgatctc atccacttgc
     4441 caatccttgc cccacgtgga cgtggtggga tcgaaaactt ggcggccatc gtgcatgtag
     4501 agcacggggt agcgcttggt tggctgcgtg tcgtagccgg gtgggaacca caccaccaca
     4561 tcgcggtcat gggccagaaa ttgcgaatga acttgcggca agtggcgata gtcaccggtg
     4621 atgcgcggcg tggctggcgc ggggcgctca tctttccagg cggccacttt gatttccacc
     4681 accgcgccat ccgccggcgt gaagacatgg ttggccatca accggccctg cgcgtccact
     4741 tcctcggttt cccaactgcc gcgggtgacc ttgaactcca cgggcgtgga atcccgcagc
     4801 cagagggtgg cttcccagcg gcagtcgtcc gtgcgcgtca tggggacggt ggccacattc
     4861 cagcggccca gctcatccgt gctgccggtc aaaaagacct gttcgcccat gcccagcgac
     4921 atggcggttt gcacaaccat caacacccgc atcatgcttg ctccttgctt gcggcgcgtg
     4981 ccgccgtcat ggttgtagcc caatccgggc gggctgtaaa tgttcgcatt gcggcgggcg
     5041 ccttgcctgc gttatgctgc cggccattat tcaggagctg ctgtccatga ttcatcccac
     5101 cgcgatcatc gaacccggcg cccaactggg cgtgaacgtc tcgctcggtc cctatgccca
     5161 cgtggcggcc acggcgcgcc ttggcgacgg ctgcgtgctc gactcgcacg cggtggtcaa
     5221 agactacacc accattggtg cgcgctgccg gctgcacacc ggtgcggtca tcgggggcga
     5281 gccgcaggat ttgtcgttca agaatgaacc gtcctatgtg gtcactggcg atgactgcat
     5341 tttccgcgaa ggggtcaccg taaaccgcgg caccaagccg ggcacaacca cgcgcatcgg
     5401 caaccatgtg ttcatgatgg ccaactccca cgccgcgcac aactgcgagg tgggcgacca
     5461 cgtgatcttg gccaacggcg ttttgctggc cggctatgtg accgtgggcg agcgcgcgtt
     5521 tctgggcggg ggcgtcggcg tgcaccagtt ttgccacatt gggcggctgg caatggttgg
     5581 cgcgatttcc gtaatcacca aggacctgct ccccttctgc gtggccgcca attgcgccaa
     5641 tagcatggta ttcgggctga atatcatcgg cctgcgtcgc gccggattca ctccggaaca
     5701 gcgcgcgcaa gtcaaacagg cattccacgt gctgtaccgc gccggcctca acaccacgca
     5761 ggcccgcgac caattgtcgg cggccaccga caatcccttt gcgcaggaat acgcggagtt
     5821 tctcagccgc gccaagcgcg gtatttgccg gttttcccac accgttgccg ccgacgaagc
     5881 cgatgcctga cgcacccgtc attcgaaccg ccatcggcgg ttttcccgac tacgaattgg
     5941 tggactccgg gcgccgctgc aagctcgaac gcttcgggcc agtgctggtg attcgcggcg
     6001 aacccaaggc ttggtgggaa cctgtactgc cgcctgccga atgggatcgc gcgcaagcca
     6061 tccacgatga agaccacggc tgggacctgc gccccggctg cccacgcgaa tggcggatgc
     6121 gctacggcaa cttgacgttt ctcgccaaga tttccgacac cagcaaacac ctcggcatct
     6181 tcccggagca agcgccgcat tggcgggcca tccagcaggc ggcggcgccc ggcgcgcgcc
     6241 tgctcaactt gttcggttat accggcgcgg ccacgttggt ggcggccgat gccggctggc
     6301 aggccaccca cgtggatgcc tccaaaccgg ccatcgcctg gggccgccag aaccaaaccc
     6361 tttcgggcat ggccgaaaaa cccatccgtt ggctggtgga agatgccatg aaatatgtcc
     6421 gccgcgaaat caagcgcggc tcgcgctatg acggcatcct gctcgacccg cccgcctttg
     6481 gccgcggccc cgatggcaac gtgtggaaag tggagcggca actgcccgag ctgctcgacc
     6541 tgagccgccg cgtcatgacc gagcgcccac gcctgctgct gttgaccacc tacaacatcg
     6601 aggcctccgc gctcatgctg cgcaatttgc tggccgatct gatgcagccg tttggcggcc
     6661 atattgaggt aggcgagttg gcccttgccc actccgccgc tcccgaccgc ttgctgccgc
     6721 tttccatcta tgcccgctgg accccgcacc cgcccccccc ctgaccccgt gcccatgccc
     6781 acactccaga ccatcggtga aaacgcccta ctgcgccgca tcctgccgcg cctgccgcaa
     6841 cggcgcgata ccattgtcgg ccctggcgac gattgcgccg tagtccgcat gcccggcgcg
     6901 cgccacgact ggctgttcac ctccgacccc gtgattgaac agcgccactt tttgccggac
     6961 acccccgcgc gcttgatcgg gcgcaaagct atcgctcgcg cggtttccga catcgccgcc
     7021 atgggcggtg agccgcgctg ggcgctgatt gatctggtgg cgcccgcggc cacgccggta
     7081 gcccgcatcc gccagcttta tgccggcatg atcgctgccg ccaaaaaatg gaacctcagc
     7141 attttgggcg gcgacaccgc cgaaggcgac accttggaat tgcatatttt cggtgtgggc
     7201 ctcattccgc gcggccgagc cgtgctgcgc gccggcgcgc gccccggcga ccggctctat
     7261 gtgaccggtg cgttgggcgg cgcgtacctg cccggctcgc gccaccacct gctgtttgag
     7321 ccgcgccttg ccgccggcca atttttggct cgcaccgggt tcgccaccgc catgatggat
     7381 ttgtctgacg gccttgccgc cgacctgccc cgcctcctgg aagcctcacg ctgcggcgtg
     7441 cagttgaatg ctgccgccat ccccatcacc cgcgcagctc gccgcacggc ccatcccctg
     7501 caacacgcca tgggcgatgg cgaagacttt gaattgctct tcaccgtgcc ggcggccaaa
     7561 caagcgcgtt ttgccaccgc ttgggctaaa cagtttcccc ggctgcgcgc cacttgcatc
     7621 ggcgaaatcc tcgccaatcc gcgccgccgc gtcatccgcc tgcccgacgg ccgcctgcaa
     7681 ccgctgcgcg ccggcggcta tcaacatttc ctcccgctcc aaggataacc atgccgcctg
     7741 ttgcctatcc ccacgaactc gaaagctatt ggttgaatct cgcccaaacg gaaacgcgcg
     7801 gcgtggcgct gcgcgatacc ttgcagcgcc tgcgtcccga cattaaacgg ctggctgacc
     7861 gcttcaccac cgcgcgcccc gccaccgaca cgctgcccga ttacctacgc gacacgcgcg
     7921 attgcgcggc ctatgcggtg ttcttcgcgc cgcaaaccca cgcgcgcctg acccacattc
     7981 tggcggaact cccccccttc ccccccaccc cccagcctct gcgcgttttg gatttgggtt
     8041 gcggcaccgg cgcggcctcg tttgcgctgc tggatcatct tggcccgcgc cccgtgttgc
     8101 tccacgcgca cgaccgctcg cgcgccgcgt tgcgctgcct gcacgaatgc ttctccacgt
     8161 tgcgcgccag ccgctggccc gccgccatgc tgcgcaccca agccgccacg ttggaagaaa
     8221 ctgccgccga tggccccttt gatgtcattt tgttgcatta cgtgctcaac gaactcaccc
     8281 ccgccctgcg ccgcagtttg ctcgctcgcg cccgcgaatg gctcgcgccc ctcggacgct
     8341 tgatcatctg cgagccgctc ctgcgcgagg ccggcgacta catgcgcgaa ttgcgcacct
     8401 tcgccttgac cgatctgggc ttgcacctgc tggccccctg cccccacgag caaacctgcc
     8461 cgctggccga accctgccat gatgtccgca cgtggaaagt gccgcagtcg ctgcaaattg
     8521 tgaacaatct cctgccgcgc gatctgcgcc acttggcgta tgccctgctg gtactatctc
     8581 ccgccccgtc gcccgccccg gatggtgtca tccgcgcgcg gttagtcggg tcgcccactt
     8641 tcgccaaagg tcaaaccttg tgtcccgcct gctgcagcaa tggcgccatc caccgcctgc
     8701 aactgctcac gcgcgacttc gataccgccg gccgcaaaaa tctgcgccat ctggagcgcg
     8761 gtcaaatcct gcgcctggcc agcctgtcgc cgctgccgga ctcctccctc tcccgcgccc
     8821 gccccgcgcc tgcggaggag gagtgaaccg ctgcgcgttc gccgcttttg cttgtccgcc
     8881 cagtggattc accgcatagt atagttggta tggaagcaac ccagcacatg ccgctcctgc
     8941 tgcagcccat ctataaggaa tacatttggg gcggcgaccg gcttatccat gaatttcgcc
     9001 ggcggctcaa tccgggcatt tatgccgagt cgtgggaaat tgccgaccac cccgacggcc
     9061 gcacgcgcct catcaatggc ccggatgccg gcgccacgct gtccacggcc attgcccgcc
     9121 gcggggcgga actgctcggg cgcggccgca ccagcgccac cttcccgctt ctggtcaaaa
     9181 ttattgacgc gcgcgaaacc ctctccgtgc aggtgcatcc ggatgacgac agcgccgcac
     9241 gcttcggcgg cgaggccaaa agcgaaatgt ggtatgtgct ctccgccacg cccgatgccc
     9301 atatttattc cggcttcaag cccggcgtga cccccgccga cttcgcgcaa gcccgtgccg
     9361 ccggcaccat tcccgcgctg ctgcagcgat tccccgcgcg ccccggcatg gtttttcata
     9421 cgcccggcgg gcgcatccac gccatcggcg ccggctgcct gctgctggaa gtacagcaag
     9481 acgccaacac cacctaccgc ctctacgatt gggaccgctt cgacaaaaac ggccagccac
     9541 gcccgctgca tatcgagcag gcgttgcagg tcatcaactg ggacctcacc ggcagccccg
     9601 tggcccccat ccaaccggaa gaagctcgtt cgccggggca tagcgcccgc ctgctgctgt
     9661 ccgcgccgca ttttcacgtg gaggaatgga ccatcactgg tcctgcccgc gccacccacg
     9721 acggcaacag ctttctgatt ctcttcgcca cgcacggcga cacgcgcatc aaaaccaacg
     9781 gccagccggc catgattatt ccgcacggca ccacctgcct gctaccggcc atcctcgaca
     9841 ccttcaccct ccagccggcg atgcctgccg gccagcccag ccgcctgctc gtggcccgcc
     9901 agccctgatt tccaccctca aaaaagcggt tttttcagcg gattggccat tgacagccgc
     9961 ccggccatgc cctagggtgg cccacgtttt tctgccggag ctaacgcccg gcaaccggcc
    10021 tcgcgccgga aacccatcag aaggcaggtg aattccccaa catgaccgaa gtgaaaatca
    10081 agaaaggcga accggttgaa aaagcgttgc gccgcttgaa aaagaaattg gaccgcgaag
    10141 gcatcatgcg cgacatccgc gccaagcggc actttgaaaa gccgagcgag aagcggcgac
    10201 gcaaagccgc gcgcgcgcgc atcaatgccc gccgggccac ttgggcggct tcgttgtaag
    10261 cggccctgcc tgcccccgag ccggatgctg gtcatccggc tcttttattt tatccggcgc
    10321 catgctacac ctcgggcatg aaaattcttg tgattaacgc cggcagttct tccatcaaat
    10381 atcaactcat ggatttggcg gacgcccacc gctacgcggt gctcgcctcc ggcttggtgg
    10441 aaaaaatcgg cgaggccacc ggccgcgtca cccacaccat cttcaccgac ggccaggaac
    10501 agcgctttgt cgaagaaaac ccgctgaaga accaccacga gggaatgtcc cgcgtggtag
    10561 cgctgctgac ggacccgcaa cgcggcgtca tccgcgacgc cgccgaaatc gagggcgtcg
    10621 gccaccgcgt cttgagctgc ggcgaggtgt atagcgacac cgtgcgcatc acccccaaag
    10681 tcaaggccac catccgcgaa tttttcccgc tgggcccgct gcacaacccg cccaacctga
    10741 tgggcattga agtggccgaa gaatttttcc cgcgcgcgcc gcaggtggcg gtgttcgaca
    10801 ccgctttcca ccagaccatg cccccaaagg cttttcttta tgccgtgccc tacgagtggt
    10861 acacgcggca ccggctgcgc cgctacggct ttcatggcac ctcgcacaag ttcatcacgc
    10921 aggccacggc ggagttcatc ggcaagccgc tggccaagac caacctcatc agcatccatc
    10981 tcggcaatgg ctgctccatg gccgccgtac gccacggcaa gtgcgtggac acctccatgg
    11041 gcgtgacccc gctggaaggg ctgatcatgg gcacgcgctc cggcgatatg gaccccgccg
    11101 cgctggaata catcatgggc cttaccggca tggacatcca ccagatggtc aacgcgctca
    11161 acaagcaatc cggcctcaag ggcatttgcg gggtcaacga catgcgcgag gtggtcgaaa
    11221 aagcggccgg cggcgatgcg caagccgaga ccgcactgca aatgttttgc taccgcatcc
    11281 agaaatatat cggcgcctat ctcgccgtgg tgccgcacgt ggatgccatt gtgttcaccg
    11341 ccggcatcgg ccaaaactcc gcgccggtgc gcgagcggat ttgcaccggg ctggggcatt
    11401 tgggcattcg gctggatttg gccaaaaatg ccgcgcgagc caacgggccg cgcgccatcc
    11461 atcaggccgg cgcgccgctg caaatcctgg tgttgcccac caacgaagaa ctgcaaatcg
    11521 cgctggaaac caagcaaaac atcactggcg cgtaagcccc cctctcgccc cgcacgcgcg
    11581 cggttttacg ggtcgcgcgg ccagggcgcc ggctgcggga ataagacttc cacccacccc
    11641 ttgaacttgg gcggcaaatt gacgccggtg ccgtcaaagt ccgtcacatc gcggccgtcg
    11701 gccaagacac ggatgggccg catattgcgc ggccaccaga gccggtagcc agcgggcggt
    11761 tgcgccccgc cgccaatttc caccgtgaat tctttttgct cccaccgccc cgccaaatcc
    11821 agcggcccga acgcggtggg catgccgaac acacggaagc cttcgccatg ctgcaaccac
    11881 tcggcgggcg cgccggcaaa caaatccaac tgcttgtcgg tttcacgggc ggctaggccg
    11941 cgcacgccca gaaaatacag cgccgccgcc cggatatccg gctgcgcggt gacatgcccc
    12001 atctggcgcg gatcgctggt gctgatatcg ggccagcttt gccaaccgcg cggctggcgg
    12061 cgatccagca gcgagtagag cacctcgcgc gcgtaatcgg tgcgccccat ggtagccaac
    12121 gggcccagca gcgcgccttc gtcattcgcc atcacgccgg accagccggg ctgccggcgc
    12181 tgcaaaaagt cggcgtagaa catgtcgagg ctggtttgca attcgtgcgg ctcaaccaaa
    12241 tcggtttcgg cgccgggcca aaacagcagc gccacggttt ggatatccaa ttgctcatct
    12301 tcggcagcgc ccggcaccca catgccggtc atcaaatcca ttcgcgcgcg aagcgagcgc
    12361 cgcacttgcg ccttcagcag gcggtactgt tcatccgccc acgtggcctc tgcttccgcg
    12421 cccagcagcg cggcggccgt gcgggtttcc ttccaaccca gcagcgccca tatctggtcg
    12481 gcatagcggt gctgcggctt ggcgctgccg gggcgcgggc ccgacgccgg taaaaggcca
    12541 tccaccaaaa agcgctgatc ctccggcagg agccaatcgg ttttttcctg gtccgcgcgc
    12601 aaccgctgca agtaggcgag cgcgttctgg agccgcggga acatcgcctg caaaaatgcc
    12661 gcatcctgcg tgaagcggta atactccagc gccatgaacg ccaactggcc ctggccggca
    12721 tattgcccca tgcgcagacg cggggcaggc gtgccatccg ccatgaacca caccggcacc
    12781 cagccatcgt cctgctgagc cgccgcaacc atttcaatcc acgggagggc cgccttggcc
    12841 tgccccgcac gcagcagcgc ggccacgcgc agcgcgatgg tttccgtgct ttcaccatcc
    12901 accccgcgca cgcccaacag ccagcccact tgagcgtgta agcattcgat ggcatcaggc
    12961 cgcgcaatgc gcggctggta gtgccctgtg gcatcccgcc agctccaggt ggcatccacc
    13021 cactcgcggc tgaacaaatc ggccaccttg cttggcgagc cgttcacctt gggccacggg
    13081 aaacggcgcg gacgcgccac acccggcggc agcgcgttac cggccaccac ccagcgcatc
    13141 cgcccgccgg gcgccagcga aaacgtgcgc caccacaggc cggaggccag cccctcggca
    13201 tcgctggcgg tgcgggcgcg cggcagttcg ccgcgcagca aggattccgc cacatcgccc
    13261 tgcgcgaacg ccaccgcgcc aaacggcatg ttggtggcgg gcaccgcgaa gagcggttcg
    13321 tcgttcaccc acatttcctg ccagccgctt tcgctcggca tcacgcggat tttataaatc
    13381 ggcgccagcc cgccgtgcgt gcccagcagc gccaaccgca cggggcgcac cacccagcac
    13441 cagcgcccgg tttgggtcat cagtgagtca tttaccagtt catacatcgt ccatgccatc
    13501 gccgccggcg cgttggtccg cgcctgcgcc tggatgcgca gtttcagccc cgaggccatg
    13561 tgccacgtga cttccggcag cggcgcgccc tccccgccca atcggtgctc cacccgctcg
    13621 gcctgctgcg cggacagcac ttggccatcc atcaacaaaa gtggcgtcaa agtgggcccg
    13681 cgcggtcgcg gcgcgaatac gccccattca tccagcagcg cttcggcatc cgtcgcggcg
    13741 gggccggcgg caacgctcca ataggtttgg cgcttgcgga acaagtcggg ataaatcccc
    13801 tccggcgcgt gcgccgcggc cacttcataa cgcgcccacg gccgcgcggt gccttcatcg
    13861 ccacgcagcg caatgcccgt caacgcgaag catccgtcat cccccgtgcc atccgcgaac
    13921 gacaaccgca catattgcgc cggcagggcg gtctccgcca tcagcacatc gaagtccccc
    13981 acctgcggtt gcagttgtcc cagccgttgc cactcggtgc gattggtgga cacctccacg
    14041 gtcaactgtt gcgcgaagcc gttggtgccc caatccacgc gcacgctgcc caccggacgt
    14101 gccgcgcgca aatccagttc caccacggcc gccgggttgg tggaaaccca cgcggtttct
    14161 tcgcggccat ccagcagcgc cgcggccttg ggatcgggtt ggccatccac ccacgcctgc
    14221 ggctggtcgg ccagcccttt gatttccaat gcgaccagtg ccgccccctg caactccggc
    14281 gccaacaaca ggcgcacatg gcgcgccagc acgggctcga agcacacttg atcccagccg
    14341 ccatcgctgg tcttggtttc gtagctcaac atccagtgca cgccatcaag gctcgtttgc
    14401 agcgcatagg ccgcggcgta cggttcgccc cactgcaacg aaaacccgca gaccatcgcc
    14461 gcgcgggcca tatccacttg cagccaagct tccttgccgc cggggcaatg ccaccacgtg
    14521 tccgcccgcc catccaccgc cagctcggcc tcgtgcccct cggccgcgcc ggatgcggtc
    14581 acgacccagc gcttttccgc cggcaccgcc aaacggttgg ccttgaccgt ggcgcatcca
    14641 accagcgccc cgcccaacaa cgccgcaccc agcgcaaccg ccaatatttt tccacactgt
    14701 ggaaaaaagt tttccacagt gtggaaaaag ttgtgcgcac gcgccatgag ccgcctcacc
    14761 cgccataaag cgccagcgtg cggcggatgc gcgccaccac gcggctcttg cccaaaacgg
    14821 ccaacatttc gaaaagcccc gggcccacgc ccgtgcccga caccgccacg cggaccgggt
    14881 gcactagatc gcccatctgc cgcccagttt gctccgccag cgaatgcagc gcggcatcgg
    14941 tggcttccgc cgtgaacggc tcgggcagtg cgaccagcac ttccagctct tcgcgcagaa
    15001 tggccagcgc gccttctttc aacaggcgct tgcgtacgct tttttcgtca tacggatact
    15061 cctccgtgaa gaaaaacgcc gcctgctcgc cgagatcgga atagagccgg atgcgctcgc
    15121 ccatgcacat gatcaccgcc tggaaataag cgggatcaat gtcctcgcgc cagaggccct
    15181 ggcgttgcat ggcggcgcga cagccagcca ccaggtcctc cactccgcgc cgtttgagat
    15241 gctggccgtt catccagagc aacttggtca tgtcgagctg gctcggcgcc tgccgcacgc
    15301 ggtcgagcgt gaaggcggca atcatttcgt cgcgcgtcaa cacttcgcgg tcgtcgccgg
    15361 ggctccagcc cagcagcgcc agcgagttga acaacgcctc cggcaaaaac cctttgtcct
    15421 gaaattcgcc gacgtacgcc gcgccgtcgc gcttcgaata cggtttgccc tgcgcgttga
    15481 caatcatcgg caggtgcgca aactgcggca tgggtgcgcc aatggccgca tacatggcca
    15541 catgcttgaa cgtgttttcc acgtggtcgt cgccgcggat cacgtgcgtg atgcccatgg
    15601 tgatgtcgtc caccacgttg ccgaggtgga acaccggcga accgtcggag cgcacgatga
    15661 caaagtcctt caagtcttcg gcctttttgg tgatttcacc tttgacgagg tcgcggaacg
    15721 aaatatccgt gcccggcatt ttgaacacga tggcctcgcc tttgcccgtg ccgcccttgt
    15781 ccgatttgta ggccgcgccc ttcgtcaaca gcatttcggc cacttcgaga tgccgtgcgc
    15841 gctgggtgct ttggtaaagc ggcgcgccgt catatgtcag ccccaaccac gccagcgaat
    15901 ccagcacggt ctgcaccgcg gccggcgtgg agcgctccag atcggtgtcc tcaatccgca
    15961 gcaaaaattc accgcccgcg tgacgggaaa acaaccagtt gtagatggcc gcccggatgt
    16021 tgccgatgtg gacattgccc gtggggctcg gcgcaaagcg cgtgcgaata gtcatgaaac
    16081 cgtgattcct ttcattcgtc gaaaacgcat actacctgcc cagcgcccgc cccacaatcc
    16141 cgcaatcacc agcgcgacca ccgcggcacc gcgcccatgc cgccggcttt ttcctcctgc
    16201 agcaggccgg gatcaatctc cgccaagaag cgcgacggcg ggcacagcat cacgccgccc
    16261 tcgcgcgttt tgcgcatccg tggctggcat agccagaggt agtccttggc gcgcgtgaca
    16321 gccacataaa acagccggcg ttcttctgct tcgttggcgg cttcttcaag tgaacgcgcc
    16381 gcggggaaca ttccatccac catccacagc acaaacaccg ctttccactc caatcccttg
    16441 gcctggtgca cggtcgaaag ccggatggtc tgcgcgggcg ggccggcgtg caggttttgt
    16501 tcggcatcca ggttggtcag tagcgccatc tcgctcagga acgcggcggt gtcgtcatac
    16561 ttggccgcaa aggtcatgag ttcctcaaca tcttccagcc gctgctccgc gttctcgaag
    16621 gtttcctccg cgtaatcgcg ataaaacgcc tccaccagcc ggaaaatcaa ctcggcgggg
    16681 cggtcgagca cgtcgccctc gccttcgccg ggcgcaaaga tttttgcatc cacctcggcc
    16741 cacaggggct gcgcgtcctt gggcagcagc ttggtcagtt gcgcgcgctt ggcggcatcg
    16801 cgcaggttca cgtgctggcc cagatcgcgc cagagcgcct gcgctttttt cggccccacc
    16861 ttgggcagca tttccatcag ccgcatgaac gcgagactgt cggccggatt gaccgccagc
    16921 cgcggcagcg aacaaacatc cttgatgtgc gcctgctcga aaaaccgcac gccgctcatc
    16981 agataataat ccaagcgcgc gcgggtcagc tccaactgga gttccatgga gtgataatgc
    17041 gagcggtaca gcaccacgat ttcgctgggc tgcgccgcgc cggaatccag caactgctgc
    17101 accttgccca cgatgtagcg cgcctgatgc ccaccatcca ccagcttgac gtacaccggc
    17161 ttgacctccg ccgggcgcgt ggcgcgcaac accttctggt attgctccgg gttgcccgca
    17221 atgacgcggt tggccacgtc cagaatcccc ggcgtactgc ggtagttgtc ttccagcttg
    17281 atgacctgcg cctcgggatg ccggcgctca aacgaaagaa tgttctgcac ttccgcgcca
    17341 cgccacgaat agatgctctg aaagtcgtcc cccaccgcca gcaagttgcc gtggcccgac
    17401 gccagcaagt ccacaaaatc attctgcaac ttgttggtgt cctgatattc gtccacaaga
    17461 acgtgctgca tccgctcctg atagcgggca gcgaccgatt cgtgttcttg caagagcttc
    17521 aggccgttga ccagcaaatc gtcaaagtcc atggcattga gcgcacgctt gcgctgctca
    17581 tagagcgtgt gcaccttcag aatggccgtc aagtccacgg ggtgcgcccc aaaccggtcc
    17641 gccaccgtct ccgccacatc cgccccacgg ttcgccgcta aactgaacac gctgcccagc
    17701 acttccgcct tgggaaactt tttgtcgcgc aggcccagct cattgatgca cgctttcagc
    17761 aggcgcgtgg aatcgtcttc atccagaatg gaataatcgg cgccgtagcc cagtagcgtg
    17821 gcgtggcggc gcagcatccg gttggcggca tggtggaacg tgccgcccca cagtccgccc
    17881 acatcctgcc caataagctg catggcgcgt tccagcattt cgcgcgcggc gcggttggtg
    17941 aaggtgagca gcagcatccg gcgcggttcc acgccgcgct ccagcgccag ccacgccaca
    18001 cggtgcgtca gcgtgcgcgt tttgccggtg ccggcggcgg caatcaccag caccgccccg
    18061 tccggcgcgg tgaccgcgcg caactgggct tcattcaggt gctgggcaaa atcacaggac
    18121 attcgcaggc ggcgttcagg atgggctggc tgtcaccagg tcagccaagg tcagattgcc
    18181 gaacccctgc tgcgcgccct gctcaatctt ggccagtgtt tccttgagcg gcgtgtccac
    18241 cgtcaggctg gcttccagtt cggcgtggct accaccctcg cgcatgagct tgttcaccag
    18301 ctcgtgcagc cggatgctgt ccggggtgcg gatgagcaca tagcttgcgt cgcggctggc
    18361 cacttgcgtc agatagccgc gccgcgccag catggccacc accgtgttga tcagccggat
    18421 gggcgccttg gtctgacggg caaactcggc cacgtcaaaa aatcctttgc cgccatccat
    18481 ggcccgcccg gcttgccgca ggatcatcag cgccaccagg atccgcgcgc gcatgctggc
    18541 agtgtcggcc gcgctttcca gccggtaggt gtccgaattt tgcagcgcaa aggtgaactc
    18601 cgcgcccagc agcagaatca cccacgtcac atagagccac agcatgaaca ccggcacggc
    18661 ggcaaaagcg ccatagattt tgttgtagtt ggtcaccccc acctgcagta cggtgaacat
    18721 ccggatccaa cccagaaaaa ttaccgccgt caacaggctg ctgaacaccg ccgggcgcaa
    18781 gcgcacacgg gtgttcggca caaacacgta cagaaagccg atggccagcc acatgatgcc
    18841 gaacgccgct acattctgca cccacgcgtt ggcatccacc ccgagtatct gctggttgaa
    18901 cgcaatccgt gccttcaacg cccccacgag accgatcagc aaaggcatca gcaccagcac
    18961 gctggtatag ttggcgatct gccgcaataa actgcggttt ttgtccacgc cccacacccg
    19021 gttgtagctt ttttccacgt tggccaacag cagcaccgcc gtcagcacga agaaagccaa
    19081 accgatccac cccagcgccg cgaagttggt ggtgttcaca atgcccagga tggtatgcac
    19141 gaattgctgc cactgatcgg gcatttcggt gaaccacgcg aactcggcca gccgctcggc
    19201 atcgaactgg cccatgccga agcccttgat cagcgcaaaa cccaccgcca aaatcggcac
    19261 cagcgaagtc aacgtcacga acgtcagcga cgaggcgtgg atcgtcaggt cgtcatccat
    19321 gtagccgcgg aacaacaaat gccccacccg gatgatccgg gccggcaaac tgcccccgcg
    19381 ttcatcgccc ggcagcgcca ccagcgcccg cgaggtaaac tttcggattt tatttacacg
    19441 atcttgcaac gtcataacgt ccgcctttcc tgccgccatg ttggcggcct tggcttccac
    19501 tccccctttg tagcacagaa ggccagtcca ccgcagctat atttgcggca gtttttcgat
    19561 aatccccgag gccagcacgt tgtcgccatc gtacagtacc gcaacttgcc cgggtgtgat
    19621 ggacagttgt ggctcatcga actccacccg aatctggccg acggcggtcg cgtgcgcagt
    19681 tgccggcgcc gctttgtgct gctggcggat ttgcaccgca caacggaaag gctgttccgg
    19741 cggccccgcc aacgccagcc aattgatctc gcccgctatc aaaacgttgt ggaacagctc
    19801 gctctgtggc cccaccacca cgcggttgcg cgccgcgtca atctccagca cataccacgg
    19861 cgtccccccc ccgcccagcg ccagcccttt gcgctggccg atggtataat gaagaatgcc
    19921 ccggtgccgc cccagcaccc gcccgtcgcg cgtgacaaaa tctccggggg cggcatcgtc
    19981 tggacgaaat aaaacgccgt agtccttgct ctccacaaaa tcctggctct ccgtctgggc
    20041 cgccacatcc gcccagccct gttcagccgc caattttttc acgtcctctt tgcgccactc
    20101 tcccaacggg aacaacaatt tggccagttg atcctgctgc aagcgcgaca aaaagtacga
    20161 ttggtctttg gttgtgtcgc acccgcggcg caacaaccaa cggccgctgg tttcatcccg
    20221 ccgcacccgc gcgtagtggc cagtggcaaa atagtcgaac tccaccccca gctctttggc
    20281 gcgcgacagc aaaaaaccga acttcatctg ctggttgcag cgcacgcagg gattgggtgt
    20341 acgcccggcc agatattcct tgcggaaata acccagtact gtttccttgt attccgcggc
    20401 cagccgcacc acttgatgct caatccctag gcgctcggca ttggccttgg ccgccgccag
    20461 atgcttggct tcccccggcc cgaaacagcc ggcgaatccc gcatccagcg ctgccacaga
    20521 gccgtcccac atgctcatgg tcaaccccgt cacccaccag ccatcgcgct tgagcagcca
    20581 cgccgccatg caggaatcca cgccaccgct caaccctacc gccacactgc gcccgttcgt
    20641 cttcacggtc gcacacttta ccccgccccc tcccgaaccg ctactgttct ttgccaaaac
    20701 ccctaaaagc aaaaatttcg gcccacgctt tacttcactt attgcaatat gtgaattcac
    20761 ttattgcaat aagtgaaata tcaaaaaatg cccagcgacc gaatgtcggg caaactgtcg
    20821 ccgacagttg ctcaacaggg gactaggaat tcgaaacgag cctcaacacg cgaggcgttt
    20881 catgaatagc gtacaatcgc ccggcaggcc ggcaaacaag gggcgcgtgg cataggccaa
    20941 gcggttcatc tcgatgtacc cctgtttacg gaaggctccg gccgacttga tgttcgtggc
    21001 ctcggaaacc aagtactcat attgcttgtc gcgcagaaga ttttccaagc gacggagcaa
    21061 cgccatggaa atccccgcgc gccaataagg ccgggcgacg gctaaataaa agacattggt
    21121 tcccttgccg ggaaagccgg ctaacgggcg gcttaactct tcaaaaaatt catccatctg
    21181 gcccagtgcc aggcgatatt ctgccggtac cacgcgcagg gtttgccgat agcgctcatc
    21241 gtctaggctc aaggcaatgc cggccaaacg gtcagtatcg gtatccaacg ccgtgatggc
    21301 caaatggtct tcaaaacatt gatcacaaat cgttcggaca tacgtcgccg caaaatctcg
    21361 cgacaggtgg agcgcctcgg tcaggggctc caaactgggg aaaacatcaa cgagcaagtc
    21421 agcagattca cgcagtcgtt cagcggaaag ttcctcaatt tgtatattca tcgggtggga
    21481 gcgcaaggcc cccgctaggt gccggcatac ttgggtggct ggagtgcggt cttgagtgtt
    21541 tggggtgatc tcagaccgcc aagtcgctgg cggtgggtga tgcggcagcc gctagtgtca
    21601 catcagcctc aaggatcgtg atgagggcgg cgcgtctcaa aaaacgagtc gtgcccgcag
    21661 gcgcataaag caaaagcccg gcaaaatgcc aggcctgatc agccgctcgc tggacaattc
    21721 ggttcattga aacagcaccc atcctacgcc ttccctaatg gctgtccagc actttccaaa
    21781 ataaacatat ttatgtatat cttattgcct tataacatgt tgcaaattca taaatggagc
    21841 tgctcggaaa tagaaaaatc aaaattatca gccggggaac gactcctagg tgtccaggct
    21901 aaggccgcaa gttttcccta atactacctg gctacaaacc ctgttggcca tccttgaaac
    21961 tacgttcttt ctacgggata tctcccgaca aatgctaaat gaaacggaag ataccgtgtt
    22021 caatggatgg gtgacgtcaa tttccagtga cggcaagccc ggcgcgggga agaacgagtc
    22081 ttgcgttctt ttcccggtca aattcaggca ataaaatgga gcgagcgatg ggatttgaac
    22141 ccacgacaac caccttggca aggtggagct ctaccactga gctacgctcg cccttctatt
    22201 caggacgccg caaatctagc atacggccct agacttgcaa ccattttttc cggccgtgcc
    22261 gtgtagtttt caaacccgca cttttttgta gttagcaaag tcgcactttt
//
LOCUS       DAOPNP010000031        21975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2964_length_21975_cov_7.840009, whole genome shotgun sequence.
ACCESSION   DAOPNP010000031 DAOPNP010000000
VERSION     DAOPNP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21975
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_21975_cov_7.840009"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..954
                     /locus_tag="PKI64_04260"
     CDS             109..954
                     /locus_tag="PKI64_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00258.1"
                     /translation="MRGWRGIWWVLALVALLLAGAFNTRLLALRSRAHLTLAPPTRNL
                     PPSVALTTVVLGGFRGVLADILWMRAGRLQDEGRFFELVQLSDWIAKLQPRSPAIWAY
                     HAWNMAYNISALMPANADRWRWVRDGIVLLRDEGLAANPGSARLYQELAWLFLHKLGT
                     DSDHAAAYYREQWAAEVASGAVSLDPARVAAIEREFGALDWRTPQAQAIYWAMAGLPF
                     ARADFDDLSLRRMVYQALLQLIAQGRRDLIPPTIRFVRETAQRHPSIGALSEVLRQLE
                     ALQTD"
     gene            complement(1123..1992)
                     /locus_tag="PKI64_04265"
     CDS             complement(1123..1992)
                     /locus_tag="PKI64_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE00259.1"
                     /translation="MKRYPAVQEKVVVVTGASTGIGRATARLLKSRGWRVLPTARSRT
                     DLDLLRGEGFQPVELDLADSDSVEYAAKEVLELTHGVIGAVVNNAGYGQPGALEDLSR
                     AAMRKQFETNVIGTLDFTNRFIPAMRAQGYGRIVLVSSVVGRVTIPFLGIYSASKFAV
                     EAIGDGLRVELGPAGISVSVVQPGPIKTSFRKRVVSEATRGLDMRDSVFAATYARELS
                     QPERTYTRPTDRFRRPPEAVAKKIVHALEAARPRVRYPVTAVAWFGDFVARFFPARLK
                     DRILSSKVIGKEV"
     gene            2125..2484
                     /locus_tag="PKI64_04270"
     CDS             2125..2484
                     /locus_tag="PKI64_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE00260.1"
                     /translation="MSKTAATPDELMSKTLPMAELVSVQPGAVVSRTVISKPAGTVTL
                     FAFDKGQGLSTHSAPYDALVWILEGAAHITIEENELVAKVGDMVIMPANRPHGLQANE
                     PFKMALVMIKEKSAAPA"
     gene            complement(2481..3866)
                     /locus_tag="PKI64_04275"
     CDS             complement(2481..3866)
                     /locus_tag="PKI64_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00261.1"
                     /translation="MKRAWTLAGWAALALALGALPGRAQSAPPGVGKPGRAAMYSADR
                     RFMVSGLTSAENMLLAGKLAELARQVEECVGRPLPMQRDQVLGVMVQSGGAPDAAIMK
                     VQGWDDGRFYQRLVVPNAVRLDEEDLFEAACWLLLNRYAADFTPAGQRTGLGAATPDW
                     ISCGMAQNAQAALKARNREWLNREQAEGRAMPLAQVIKLELLPSGRWREKAYAAAAVD
                     FLFPAGQPADWQALFAAVGARQPIDAAWLRDHAAALQGQKPEDAWRAFLAERRRQRSV
                     EAWSDRGLLLEERLLQVLNFRPRDFAPEIPADVPHDLFARDLIEFRAQSWVAPLAAAL
                     SLQVQSLKLGAPPALQEVLGAYAQYFDHLTLRPAEKHSWWQRAPKSAAKAQPRDDATW
                     EVALNQLWARAERAHQNYLATTQSRKQYVDQFDRGDDGWSEPPPAAGDVPRTAIQKYM
                     DEAEERLRPAR"
     gene            complement(3869..5536)
                     /gene="ettA"
                     /locus_tag="PKI64_04280"
     CDS             complement(3869..5536)
                     /gene="ettA"
                     /locus_tag="PKI64_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOE00262.1"
                     /translation="MAGEYVFNLTHLSKQYDAVTVLDDITLAFFFGAKIGVIGGNGAG
                     KSSLLKIIAGLDKEYHGECIVAPGIRVGYLPQEPQLDETKDVAGNVAEGAAAAQAILD
                     RYDELCGKLGEALSDSESEKLNNELGRLQDLIDARDLWSLDSQIERAMDALRLPPGDA
                     DVRTLSGGEKRRVALCRLLLSNPDALLLDEPTNHLDAETVAWLEDYLKKFSGTLVVVT
                     HDRYFLNNVTEWILEMDAGRGYPFKGNYEKWLETKQAMASAQNKQHAARQKMLQRELE
                     WIRLNPSGRQSKSKARLARYEELAAQQVDTREDVLEIQIPAGKRLGDLVVRAEKLRKV
                     YGNRLIMDNVSFNLPRGGIVGVIGPNGAGKTTLLRMIVGEEKPDGGTLTIGETVELSY
                     VNQFRDALDDRKTVFEEITGGQETLDLGGRAMNSRAYCTRFNFRGTDQQKKVGELSGG
                     ERNRVHLAKLLRRGGNLLLLDEPTNDLDVTTLRALEEGIQSFGGCAVVVSHDRWFLDR
                     IATHILAFEDDGTVTWFEGNFEGYHEQRRRLLGDAADQPKRIRFRPI"
     gene            complement(5565..6680)
                     /locus_tag="PKI64_04285"
     CDS             complement(5565..6680)
                     /locus_tag="PKI64_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00263.1"
                     /translation="MSDISRALQNKLSRKGQLKEQAEEQLDLFASLADHTPTPRFITP
                     PPPATPRPEPAMILPPATPKSAPSEPPTDIMADVRAALSDSSAPRPSEPAPDNGLETR
                     PPLRTGIYHRPQRLAFSLPEPPPPPPPGSSPWQKIKAWFAGAELDRRMIALIVVLLAL
                     AALIGFWSGCPGRAPESAATAESAEGPASETAASATPAQPATPTTPVPATPAPASPAI
                     PAASAKPAATLAAADATWQIAGAESFQKEGGIFIRYAAPIFVSADNISVEGMRALKAT
                     AARLARLENGARVQVTGHTDDVPLSAPTAKFKSNEDIAAARAKVAMEHLSAMTRANKK
                     LSFEPVTGAPQDAPFPNDTPQNRRLNRTVTVQVFPAP"
     gene            complement(6705..9308)
                     /gene="leuS"
                     /locus_tag="PKI64_04290"
     CDS             complement(6705..9308)
                     /gene="leuS"
                     /locus_tag="PKI64_04290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOE00264.1"
                     /translation="MAYPHSEIEAKWQAHWKQHKTFRTPDNVDQSRPKFYVLDMFPYP
                     SGDGLHVGHPEGYTATDILARYYRMKGVNVLHPMGWDSFGLPAEQYAIKTGKHPRIIT
                     ASNIDTFRRQLQMLGFSYDWDREIATTDEAYVRWTQWIFLQLFKRGLAYESEMPVNWS
                     PDLCAVLANEEVEEWRAKGFTVERRPMRQWVLKITQYAERLLADLDGLDWPENIKEMQ
                     RNWIGRSEGAEVDFALGTETVRVFTTRPDTLFGATYMVLAPEHPLVDKITTAEQRAAV
                     QAYRVAAAGKSDLERTELAKEKTGVFTGAYAVNPVNNQPIPVWVADYVLWGYGTGAIM
                     AVPAHDTRDFEFATKFNLPIVQVVEPPAGVDWRGYVDDGIAVNSGPYNGLPTAEFKKQ
                     ITAWLAQQGKGAAKVNYKLRDWLFSRQRFWGEPFPLLHCANCGVVPVPEDQLPVRLPE
                     MKDYRPTPDGQPPLARATDWVQTTCPTCGGPARRETNTMPQWAGSCWYYLRYLDPRNE
                     QALVAPDKEKYWLPVDLYVGGAEHAVLHLLYARFWHKVLYDCGVVSTKEPFQRLVNQG
                     MILGEMEYTGYRDAAGAWVSAEFADDNNTDRRTRAKLTAVRVPEAELQKKGEGFVLLA
                     QPEIRVVARAHKMSKSRGNVINPDDIVKEYGADSLRLYEMFMGPLTQVKPWSMKGVEG
                     VSRFLSRVYRLFVDEETGAPSPKLSAAEPTRAQLRALHAAIKKVSHDIEKMEYNTAIS
                     AMMIFVNEAQGWPELPRALLGPFVLILSPFAPHLAEEMWSRLGHAESLAYEPWPAWDE
                     DILREDEIEVPVQVNGKLRGRIRVAADAPEADIIAAALACPEAASFLAGKEIKKKIYI
                     PRRMVNLVVAG"
     gene            complement(9422..10807)
                     /locus_tag="PKI64_04295"
     CDS             complement(9422..10807)
                     /locus_tag="PKI64_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOE00265.1"
                     /translation="MKHATLTSKWWTLAVVGSGTFMSALDTSVVNVALPVISRETGAT
                     VASLEWVMLAYLIVVSSSLLAFGRLADIHGKRRIYLMGIVIFTLGSLACSLSRTIGLL
                     IASRALQALGAAMLFALSPALLVAAFPSSERGRALGMQATLTYLGLALGPPLGGFLTQ
                     HFGWPTIFSINVPIGLAMWGLALVVLHPDRNGAGQPFDPIGAGAMAIGLAATLFALSK
                     GGEMGWRHPLIITAALTGLLALVALLICEQRCRYPALDLKLFRNRRFAASMAAAWLSY
                     VCAASLSFLMPFRFLLGAGWEPARVGVWMMAVPVGMVPVAALSGYVSDKVGVTLPATL
                     GMLLVATGNACLAGFGVESPALAAVFLALTGLGAGLFTAPNNSAIMGSAPAARHGVAG
                     AMLAAARTTGFASGIAMAGLIYMTRAGHTSTPVAADVVAAVHNGLWVTAGAALLAALG
                     SLLRGPRHEAV"
     gene            complement(10820..12085)
                     /locus_tag="PKI64_04300"
     CDS             complement(10820..12085)
                     /locus_tag="PKI64_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00266.1"
                     /translation="MKWNWAKHDEGGLVRHGLLLVVATFLGALCNAVLHMALGRLLPN
                     AEYGAFIAMLGLIMVVATPMLALQNTLAHFVSHFVQENRASEIRPLFFHWAKIFTALT
                     VVLITAAWLGRGKLAAVWQVSPGLIVLTFAVLAASLWMYLFLGLLQGRQWFVWLAWAP
                     QAWGVVRLVLGVIFAYWISATAFAALAAQGFGVLSVLALCLLATLLMRLPRGAGHVRP
                     AETYRYLGSAVVCLAGYAMLMNLDTTLAKLYFDEEVAGLFAKAATIARTAVFLPVPVA
                     AALFPKVTSTGELTDVSWRLLLRGLAFSGLLIAAVVGVCLLWPALPWTILYGRWTAED
                     AAFAAQLTRGMVLAMCPLALAYLMLNFEMAQRRFRWCYALVPCGLFYVGGVALFHQHP
                     LQIALLLGAMNLLAAVLLTTGIWTQRRRG"
     gene            complement(12082..>12654)
                     /locus_tag="PKI64_04305"
     CDS             complement(12082..>12654)
                     /locus_tag="PKI64_04305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00267.1"
                     /translation="RPIGKGGAIMAAGRQARGDYIGFVDADGATPPAAFQDLVDNIGQ
                     AGLIIASRRLPGAVVSPRQPWPRRVASRIFNWLVRRLFKLQITDTQCGAKLMTAVAWR
                     DIVPRIGLTKWAFDVDMLFQTRRARYPITEIPTTWSDVAGSKLRVGRVSAQMFLAICR
                     LRLLYSPLSWVVTLYDRVLGRVIDLPPDAR"
     assembly_gap    12655..12754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12755..14433)
                     /locus_tag="PKI64_04310"
     CDS             complement(<12755..14433)
                     /locus_tag="PKI64_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00268.1"
                     /translation="MEAEQNVRAQDVPDWHWGHLALFLAVVATWLALAGLNLWMGELN
                     QDEGWYLYAAKQMGEGLWPYRDFAFTQAPMLPLVYSLVYGWVGKYGLLGGRAVTWGFG
                     TLGTLFAVWLAMRAGPRRALRFTGGLCFILIAVNVYQSYFTTVVKTYSLASLFLSAGL
                     LALAYVGPRRGFRAATLAGFLLACATATRISLGAALAVGGLYLFICRRRLRLWAWLDF
                     ALGGLLGLAVTFLPFMMLGREGFQFGVLEYHTLRESGPWLTQLAYKAGCLSRLGQAYF
                     PAAAAGLALLMLAGRYVVLRGKSPCDRGEDSPLYAGPEDIAPCFNTFLWVTVFALALV
                     HLAAPFPYDDYQVPIYPVYCAALTATAARWWLREEGRRFGETPPWRGLRRLAILVVAW
                     LLCSLYAFSSPQTQSWFVAGRDRIWWRLRSQSPLGQLRDTAQWIRDLNKPTGATELLT
                     QDTYLAVETGLDVPHGMEMGPFSYYPDWPREKAEKIGVLNRDMMLELLATQTNAPIAA
                     FSGYSLAIRSPEVEEVTREDQQAFADILAARYEHVESIPGFGQAGTTLELWR"
     gene            complement(14521..16230)
                     /locus_tag="PKI64_04315"
     CDS             complement(14521..16230)
                     /locus_tag="PKI64_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOE00269.1"
                     /translation="MLGLIFLGCCGLFWAAYKIYGGWMERRLDMLEERLTPAHTMYDG
                     VDYAPAPDAVVFGHHFSSIAGAGPIVGPIAAALAFGWGPALVWIVLGVIFIGGVQDYT
                     SMMASVRNKGQSLAQIGRSAMGPLTYRLFLLFILLVLLYVIMVFLDMTAATFAPITLK
                     WGAPETVESRIGGVVATASVFYIFLAAVFGWVLNRWKMPLRKATLLAVPLVFGGLWLG
                     QVWPLAPSLVPSLLGSDKYFWSAILLVYCLVASALPVWALLQPRDYLSSFLLYACLLG
                     GAVGLLLGSGSGAATIAWPAFITAQTPADGWIYPALCVMIACGAVSGFHAVLASGTTS
                     KQLNRESSVKRVGLGAMLVEAVLAVLALATVMILAEKPALSPPAIFAAGIERFFRPLG
                     VPDGWIMSLAMLAVSTFVLTTLDTCTRLCRIILQELTGFGSETTWKRVVSTVVVLALP
                     AYVVFQEIPGAGGQLMPAWRAIWPAFGATNQLMGAMALLMVYGWLRNQGKSAWFVWLP
                     MVFMFVTTMLALAQIVWRNFQQDGSKLIGGLSAVLLALAAVVLVDVAMRVVRRWSPLF
                     SRG"
     gene            complement(16657..19206)
                     /locus_tag="PKI64_04320"
     CDS             complement(16657..19206)
                     /locus_tag="PKI64_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOE00270.1"
                     /translation="MRIKEFLNHNLLLVDGAMGTQIQNRNPGAAAWGAYEGCNEWLNL
                     SAPEIIRDIHAAYFAAGCDAVETNSFGASPLTLGEYQLTARAREIARAAAQIARGAAN
                     EAAQQSGRPRYVLGSVGPGTKLVTLGQVTYDELYGGYQEQMTGLLEGGADGLIIETCQ
                     DLLQIKAAVAAAQKVMGANTDKLLYVSVTVETTGTLLVGSSIGAVMAALEPYPIDVLG
                     LNCATGPEAMERHLEAIARQWRGGLGCMPNAGLPTLQEGRVHYPLGAADFARIFAPMV
                     QRLGLNVAGGCCGTTPEHIRELRRALGAAPAPAPRDVQPPEQVASLFAPMDLTQNPPP
                     MYVGERANATGSKKFREALLAEKLDTAFDLLVEQDEGVAHVLDLSVAYAGRNETADMT
                     ALVARAARECRLPIMVDSTQLDVVEAALKLHGGRMLINSINFESGEEKAEKLVALAKQ
                     FGAGLVALTIDEVGMGMTADRKVEIARRLVDFCVQRGMRPGDLLIDPLTFTIGSGDPA
                     LKTAAVETIEAIRRIKQEIPGVRTLLGLSNISFGLKPAARKVLNTVFLDRCVKAGMDA
                     CIINTSALVALTDLAPAAVTLAERLLDNDAAQGDPLEVYIQHFETTSPEEEAAAEAAL
                     QTPAEQVQAALVKGKAALLADSVPALLAEMAAEDVLNNHLIPGMKEVGRLFNDGILQL
                     PFVLKSAEVMKKAVDMIKPFLKAGEAKAAGTLVIGTVAGDVHDIGKNLVDIILSNNGY
                     KVVNLGVKVSIERMLEVVRETKADMLGMSGLLVKSVQVMRDNLKAMEAAGLNIPVLLG
                     GAALTENFVINDCQPAYSGPVRYCKDAFDSITAFRELEETGRLAPTPPPKA"
     gene            complement(19299..20372)
                     /locus_tag="PKI64_04325"
     CDS             complement(19299..20372)
                     /locus_tag="PKI64_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOE00271.1"
                     /translation="MLAVLAAAVLPGAASLFQIDVDPFYADTLLKGKFGDVPVYFFLG
                     PSPTMEKAVVAFIGAATQTLDVCVYDLNLPEFAQALLDARARGVKVRVIVAQENAEKA
                     YAISGQLAQMEKLGMLTLAHNRSGLMHNKFMVADKRLVWTGSYNLTRNCSRFNDNHAI
                     VLESKELAENYSLEWLEIFEKRHGKRFAYPTPHPEVNVGGVLIHNYFTPEDDTRGGIV
                     AEMDKATNEIAILAFSFTDAAMTEAVKRAIGRGVRTIIVLDSGMATHPSANTRQLEAM
                     GANVRMSPGVLLHHKVIVIDRQTVVLGSANFSQAAFDKNDENVLIVKAPNFAQAMLRE
                     AMRCWRAEPYWITKWRTQLPASP"
     gene            complement(20505..>21975)
                     /locus_tag="PKI64_04330"
     CDS             complement(20505..>21975)
                     /locus_tag="PKI64_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE00272.1"
                     /translation="RQTGTSTLRALLYMDEIFGYFPPTSNPPAKAPMLTLLKQARAFG
                     LGIVLATQNPVDLDYRGLANTGTWLIGRLQTERDKMRVIEGLTSATAGEGLDRATLDR
                     LMANLGSRIFLMRNVHDDAPVLFETRWCLSYLRGPLAPAQISKLMPQQQSAPPAAPSV
                     VPAVAPAAPVAMPAAPAPAPKTVLAASIPQRYWPATQAPAGAAITYRPALMASARIHF
                     ADLKYGLDSWETVRHLVWTDPKHGHLVWTAVSNAPAGDDRDPLPGAAHENVPTDLADP
                     KRYAQHEKDYKAFLAEAFARELFTLPEHKLTSQPGETEGDFRIRANQLLREKRDEAMQ
                     KMRQKYAQKLTTLAERERRAVAKLENEKAQLSRQRMGTMVNFGAAALSVLLGRRMGGV
                     GRATSGINSMSRTSKEKLDVAQAGETLEAIRQQRAALEAELEQEMAATGAAFDPANLK
                     VSTVRVAPRRADTSIQQIGLAWIPCVADVSGTLRPAIAL"
BASE COUNT         4019 a         7273 c         6775 g         3808 t
ORIGIN      
        1 ccacggtgtg gaaaaatttg ggctgcgggg cgttgttttt tccacagtgt ggaaattatt
       61 tttccacagc gtggaaaagt atgccgctga cttgggtgga gccgtagcat gaggggatgg
      121 cgcggcattt ggtgggtgtt ggcgctggtg gcgctgctgc tggccggggc gttcaatacg
      181 cgcctgctgg cgttgcggag tcgcgcgcac ctcacgctgg ccccgcccac gcgtaacctg
      241 ccgccgtccg tcgcgctgac caccgtggtg ctgggcggct tccgcggcgt gctggcggac
      301 atcctttgga tgcgcgccgg acgcttgcag gacgagggcc gctttttcga gctggtgcaa
      361 ttgtccgact ggatcgccaa gttgcagccg cgcagcccgg ccatctgggc gtatcacgcg
      421 tggaacatgg cctacaatat ctccgcgctg atgccggcca acgccgaccg ctggcgctgg
      481 gtgcgcgatg gcatcgtgct gttgcgcgat gagggcctcg ctgccaatcc cggctccgcc
      541 cgcctttatc aggaattggc gtggcttttc ctgcacaagc ttggcaccga ttccgaccac
      601 gcggcggcat actaccgcga gcaatgggcc gcggaagtcg ccagcggcgc ggtgagcctc
      661 gacccggcgc gcgtagccgc catcgagcgc gagttcggcg cgctggattg gcgcacaccg
      721 caggcacaag ccatctactg ggccatggcc gggctgcctt ttgcccgcgc ggattttgat
      781 gacctctcct tgcgccgcat ggtgtatcag gcgctgctgc agctcatcgc acagggacgc
      841 cgcgatttga tcccccccac gattcgattt gtccgcgaga ccgcgcagcg gcaccccagc
      901 atcggcgcgc tgtcggaagt gctgcgccag ctcgaagcgc tgcaaaccga ctgagcccct
      961 cccaaaaaat atcgttatcc gggttgggtt gtagggctgg aatggtcgca gaccttccag
     1021 ccgttcccgg cgggaaggtc tcgttcctca accattcttg ccctacaaaa gcatccggtt
     1081 tgcttcggag tgtcccaata cgaaactcag gaccgctccc gcttacactt ccttgccgat
     1141 gaccttcgag ctgaggatcc ggtccttcag ccgcgccggg aaaaagcgcg ccacaaaatc
     1201 gccgaaccac gccacggcgg tcacgggata gcgcacgcgc gggcgagcgg cttcgagggc
     1261 gtggacgatt tttttcgcca cggcttcggg cgggcgacgg aagcggtccg tggggcgcgt
     1321 ataggtgcgc tccggctgcg acagctcgcg cgcgtaggtg gcggcaaaaa cggagtcgcg
     1381 catgtcgagg ccgcgcgtgg cctcgctgac gacgcgcttg cggaagctgg tcttgatggg
     1441 ccccggctgc accaccgaaa ccgaaatgcc cgccggcccc agctccacgc gcaggccatc
     1501 gccgatggct tccaccgcga acttgctggc ggaatagatg ccgaggaacg ggatcgtcac
     1561 gcgccccacc acggagctga ccagcacaat gcggccatag ccctgcgcgc gcatcgccgg
     1621 gatgaagcgg ttggtaaagt ccagcgtgcc aatcacgttg gtttcaaatt gcttgcgcat
     1681 ggccgcgcgc gacaaatcct ccagcgcgcc cggctggcca tagcccgcgt tattgaccac
     1741 cgcgccaatc acgccgtgcg tgagctctag cacctccttg gcggcatatt ccaccgagtc
     1801 ggagtccgcc aggtccagct ccaccggctg gaagccctcg ccgcgcagca aatccaaatc
     1861 ggtacggctg cgggccgtgg gcagaacgcg ccagccgcgg ctcttgagca gccgcgccgt
     1921 ggcccgcccg atgcccgtgg atgcgcccgt caccaccacc actttttcct gaactgctgg
     1981 atatcgtttc atgcggccat tctacccgcc ggcgcgcgtt cgtccacgga tttccaccac
     2041 ggcgcgccgg caccagttgc caaatcccgt tttcgtggta cggtgtgggc cttcaacgtg
     2101 aacaaccccg agaggaaaaa taccatgtcc aaaactgctg cgacccccga tgaactgatg
     2161 tccaaaaccc tgccgatggc cgagctggtg agcgtccagc ccggcgctgt ggtcagccgc
     2221 accgtgatca gcaagcctgc gggcactgtc acgctgttcg cgttcgacaa ggggcagggt
     2281 ctcagcaccc actccgcgcc ctacgacgcg ctggtgtgga ttctggaagg cgccgcgcac
     2341 atcaccatcg aagaaaatga gctggtggcc aaagtcggtg acatggtgat catgcccgcc
     2401 aaccgcccgc acgggctgca agccaacgaa ccattcaaga tggcgttggt gatgatcaag
     2461 gaaaagtcgg ccgcgcctgc ttaacgggcc ggccgcaacc gttcttcggc ttcatccata
     2521 tatttttgga tggcggtgcg cggcacatcg ccagcagcgg gcggcggctc gctccagcca
     2581 tcgtcgccgc gatcgaactg gtccacatac tgcttgcggc tttgcgtggt ggccaaatag
     2641 ttctggtgcg cccgctcggc gcgcgcccag agctgattca acgccacttc ccacgtggcg
     2701 tcgtcgcgcg gctgcgcttt ggcggcggac ttgggcgcgc gctgccacca tgaatgtttt
     2761 tcggcggggc ggagcgtcag gtggtcaaaa tattgggcgt aggcgccgag gacttcctgc
     2821 aacgcgggcg gtgcgccgag cttgagactc tgcacttgca gggatagcgc agcggccagc
     2881 ggcgccaccc acgattgcgc gcggaactcg atcaagtcgc gcgcaaagag gtcgtggggt
     2941 acatcggcgg gaatctccgg cgcaaaatcg cgtggccgga agttcaagac ttgcagcagg
     3001 cgttcttcca gcagcaggcc gcggtcgctc caggcctcca cgctacgctg ccggcggcgc
     3061 tccgctagaa acgcgcgcca ggcgtcctcc ggcttttggc cttggagcgc ggcggcatgg
     3121 tcgcgcagcc acgcggcatc aatgggctgc cgcgcgccga ccgccgcgaa cagcgcctgc
     3181 caatcggcgg gctggccggc gggaaagagg aaatctacgg cggcggcggc gtaggctttt
     3241 tcgcgccagc gaccgctggg cagcagctcc agcttgatca cctgcgccag cggcatcgcg
     3301 cggccctcgg cctgctcacg gttgagccat tcgcggttgc gcgccttcaa tgcggcctgc
     3361 gcattttgcg ccatgccgca ggaaatccaa tccggcgtgg ccgcgccgag gccggtgcgc
     3421 tggcctgcgg gggtgaagtc cgccgcgtag cggttcagca gcagccagca ggcggcctca
     3481 aacaaatctt cttcgtcgag gcgcacggcg ttgggcacca cgaggcgctg gtaaaaccgg
     3541 ccatcgtccc agccctgcac tttcattatg gcggcatcgg gcgcgccgcc actttgcacc
     3601 attacgccga gcacctgatc ccgctgcatg ggcagtgggc ggccaacgca ttcctccacc
     3661 tgccgggcca gctcggccag cttgccggcc aacagcatgt tttcggcgga ggtaaggccg
     3721 ctgaccatga agcgacggtc agcgctatac atggcggcgc ggccgggctt gcccaccccc
     3781 ggcggcgcgg actgcgcacg gccgggcagc gcgcccaacg ccagcgccag cgccgcccac
     3841 ccggccaatg tccacgcgcg tttcatgctc agatgggccg gaagcggatg cgcttgggtt
     3901 ggtcggcggc atcgcccagc agccgccgcc gttgttcgtg gtagccctcg aaattgccct
     3961 cgaaccacgt gacggtgccg tcatcctcga aggccagaat gtgcgtggcg atgcggtcga
     4021 ggaaccagcg gtcgtggctg accaccaccg cgcagccgcc gaacgactgg atgccttctt
     4081 ccagcgcgcg cagcgtggtg acgtcgagat cgttggttgg ctcgtcgagc agcaggaggt
     4141 tgccgccgcg ccgcagcagc ttggccagat ggacgcgatt gcgctcgccg ccggacagct
     4201 cgcccacctt tttctgctgg tcagtgccgc ggaaattgaa gcgcgtgcag taggcccggc
     4261 tattcattgc gcggccgccg agatccagcg tttcctgccc gccggtgatt tcctcaaaca
     4321 ccgtcttgcg atcgtcgagc gcgtcgcgga actgattgac gtaggacagc tccacggttt
     4381 cgccaatggt gagcgtgccg ccgtcgggtt tttcttcgcc gacaatcatg cgcagcagcg
     4441 tggttttgcc cgcgccgttg gggccgatca cgcccacgat gccgccgcgc ggcaagttga
     4501 aactgacgtt gtccatgatg agccgattgc cgtacacctt gcgcaatttt tcggcgcgca
     4561 ccacaaggtc ccccagccgc ttgccggcgg ggatttggat ttccagcacg tcttcgcggg
     4621 tatccacctg ctgggcggcc agctcttcgt agcgggcgag gcgcgccttg ctcttggatt
     4681 ggcggccgga cggattcagg cgaatccact ccaattcgcg ctggagcatt ttctggcgcg
     4741 cggcatgctg cttgttctgc gcggaggcca tggcctgctt ggtttccagc cacttttcat
     4801 agttgccctt gaagggatag ccgcggcccg cgtccatttc cagaatccat tcggtgacat
     4861 tattgaggaa gtagcggtcg tgggtcacga ccaccagcgt gccgctgaac ttcttgaggt
     4921 agtcctcgag ccaagccacg gtttcggcat cgaggtgatt ggtgggctcg tcgagcagca
     4981 gggcatcggg attggaaagc agcaggcggc agagcgccac gcggcgcttc tcgccgccgg
     5041 aaagcgtgcg cacgtcggca tcgcccggcg gcaggcgcag ggcatccatc gcgcgctcga
     5101 tctggctgtc gagactccac aaatcgcgcg catcaatcag gtcttgcagg cggcccagct
     5161 cgttgttgag cttttcggat tcgctgtcgg aaagcgcctc gcccagcttg ccgcacagct
     5221 cgtcatagcg gtcgagaatg gcctgcgcgg cggcggcgcc ttcggcgaca ttgccggcga
     5281 catccttggt ttcgtcgagc tgtggttcct gcggcagata gcccacgcgg atgcccggcg
     5341 ccacgatgca ctcgccgtga tattccttgt ccagcccggc gatgattttc agcagcgagg
     5401 acttgcccgc gccgttgccg ccaatgacgc cgattttggc gccaaagaaa aacgccagcg
     5461 tgatgtcgtc gaggaccgtc acggcgtcgt actgcttgga gaggtgcgtc aggttgaaga
     5521 catattcgcc agccacggga aagctccttt cgtcagcgcg ccggtcacgg cgcggggaaa
     5581 acctgcaccg tcacggtgcg attcaagcgc cggttctggg gggtgtcatt cgggaacggc
     5641 gcatcctgcg gcgcgcccgt caccggctca aacgacaact ttttgttggc gcgcgtcatg
     5701 gccgacaaat gttccatggc caccttggcg cgggccgccg cgatatcctc gttgctcttg
     5761 aacttggcgg tgggggcact cagtggcaca tcgtcggtat ggcccgtcac ctgcacgcgc
     5821 gcgccgtttt cgaggcgcgc cagccgggcg gcggtggcct tcagcgcgcg catcccttcc
     5881 acggaaatgt tgtcggcgga aacgaagatc ggcgcagcat agcggataaa aatgccgccc
     5941 tctttttgga aggattccgc gccggcaatt tgccaggtgg catcggccgc ggcgagggta
     6001 gcggcgggtt tggccgacgc ggctggtatt gctggtgatg cgggcgcagg cgtggcaggc
     6061 acaggggtgg ttggcgtggc gggttgcgca ggcgtggcgg atgccgcggt ttccgacgct
     6121 gggccttcgg cagattctgc cgtggccgcc gactctggcg cgcggcccgg gcagccgctc
     6181 cagaaaccaa tcaaggccgc caacgccaac aacacgacga tcagcgcgat catccgccga
     6241 tccagttccg cgccggcaaa ccacgctttt attttttgcc aaggcgaact tcccggcggc
     6301 ggggggggcg gcggctccgg cagcgaaaaa gccaaccgct gcgggcggtg ataaatcccg
     6361 gtgcgcaacg gcgggcgggt ttcaaggccg ttgtccgggg ccggctcgct cgggcgcggc
     6421 gcgctggaat ccgacaacgc cgcgcgcaca tcggccatga tatccgtcgg gggctcgctg
     6481 ggcgcgcttt taggcgtggc gggtggcagg atcatcgccg gctccgggcg cggcgtggcc
     6541 ggcggcgggg gcgtgatgaa acgcggcgtg ggggtgtggt ccgcgagcga agcaaacagg
     6601 tccaactgct cctccgcttg ctccttgagc tggcctttgc ggctcagctt gttttgcaac
     6661 gcgcgggaga tatcgctcat ggccgttcct ttgtttgctc cgccctagcc ggccaccaca
     6721 aggttgacca tgcgccgcgg aatatagatt ttctttttga tttccttgcc ggcgaggaag
     6781 gacgcggctt cggggcaggc cagcgcggcg gcgatgatgt ccgcttcggg ggcatccgcg
     6841 gccacgcgaa tgcgtccgcg cagcttgccg ttgacctgca ccggcacttc aatttcgtcc
     6901 tcgcgcagaa tgtcctcatc ccacgcgggc cacggctcgt aggccaggga ttccgcgtgg
     6961 ccaaggcggc tccacatttc ttcggcaaga tgcggcgcga aggggctcag gatcaacaca
     7021 aacggcccca gcagcgcgcg cgggagctca ggccagccct gcgcctcgtt gacgaaaatc
     7081 atcatggccg agatggccgt gttgtactcc attttttcga tgtcgtggct gaccttcttg
     7141 atggcggcgt gcagcgcgcg cagttgggcg cgggtgggtt cggccgcgct gagcttgggc
     7201 gacggcgcgc cggtttcctc atccacgaac aggcggtaaa cgcggctcag gaagcgcgag
     7261 actccctcca cgcccttcat gctccacggc ttcacctgcg tgagcgggcc catgaacatt
     7321 tcatagaggc gcagggaatc cgcgccgtat tccttgacga tgtcgtcggg gttgatgacg
     7381 ttgccgcggc tcttgctcat cttgtgggcg cgggcaacaa cgcggatttc cggctgcgcc
     7441 agcagcacga agccctcgcc ctttttctgc aactctgctt ccggcacgcg cacggcggtg
     7501 agcttggcgc gcgttcggcg gtcggtatta ttgtcgtcgg cgaactcggc agagacccac
     7561 gcaccggcgg cgtcgcggta gccggtgtat tccatttcgc cgaggatcat gccttggttg
     7621 acgaggcgct ggaacggctc cttggtggac accacgccgc aatcgtagag caccttgtgc
     7681 cagaagcggg catagagcag gtgcagcacg gcgtgttccg cgccgccgac atacaggtcc
     7741 accggcagcc agtatttttc cttgtccggg gcgaccagcg cctgctcgtt gcgggggtcg
     7801 agatagcgca ggtagtacca gcacgagccg gcccattgcg gcatggtatt ggtttcgcgc
     7861 cgcgcggggc cgccgcaggt ggggcacgtg gtctggaccc agtcggtggc gcgcgcgagc
     7921 ggcggctggc cgtcgggcgt gggacggtag tccttcattt caggcaggcg cacggggagc
     7981 tgatcctccg gcaccggcac cacgccgcaa ttcgcgcaat ggagcagcgg gaacggctcg
     8041 ccccagaacc gctggcggct gaagagccag tcgcgcagtt tataattcac tttggccgcg
     8101 cccttcccct gctgcgcgag ccaggcggtg atttgctttt tgaactcggc agtgggcagg
     8161 ccgttgtagg ggccagagtt gacggcgatg ccgtcgtcca catagccgcg ccaatccacc
     8221 cccgccggcg gttcgaccac ctgcacgatg ggcaggttga acttggtggc aaactcgaag
     8281 tcgcgcgtgt cgtgggcggg cacggccatg atggcgccgg tgccgtagcc ccagagcacg
     8341 taatccgcca cccacacggg aatcggctgg ttattgacgg gattcaccgc gtaggcgccg
     8401 gtgaatacgc cggttttctc cttggccagc tcggtgcgtt ccagatcgct cttgccggcg
     8461 gccgccacgc ggtaggcctg caccgcggcg cgctgttcgg cagtggtgat cttgtccacc
     8521 agcgggtgct ccggcgccag caccatgtag gtggcgccaa agagtgtgtc ggggcgcgtg
     8581 gtgaacacgc gaacggtttc cgtgccgagg gcaaaatcca cttccgcgcc ttcgctgcgg
     8641 ccaatccagt tgcgctgcat ttccttgatg ttttcgggcc agtcgaggcc gtccagatca
     8701 gccagcagtc gctcggcata ctgggtgatc ttcagcaccc actggcgcat ggggcgacgc
     8761 tccacggtga agcccttggc gcgccattcc tccacttcct cattggccag caccgcgcag
     8821 aggtcgggac tccagttgac gggcatttcg ctttcgtagg caaggccgcg cttgaacagt
     8881 tgcaggaaaa tccactgggt ccagcgcacg taggcttcgt cggtggtggc gatctcgcgg
     8941 tcccaatcat aggaaaagcc gagcatctgc aactggcggc ggaaggtgtc aatgttgctg
     9001 gcagtgatga tccgcggatg cttgccggtc ttgatggcgt attgctcggc gggcaggccg
     9061 aacgagtccc agcccatcgg gtgcaggacg ttcacgccct tcatgcggta atagcgcgcc
     9121 aggatgtcgg tggcagtgta gccctcgggg tggcccacat ggaggccgtc gccgctggga
     9181 taagggaaca tgtcgagcac ataaaacttg ggccggctct ggtccacgtt gtccggcgtg
     9241 cggaaagtct tatgttgttt ccaatgagcc tgccatttgg cctcaatttc agaatgcgga
     9301 tacgccatgt ttttacctca aatcaataat gacgggcact ttagccccgc gcccgccctg
     9361 agtccattgc ttttcgcgcg gcgtggcgcg cgcgacgccc gcggcagctc agttgggcgg
     9421 tttagacggc ctcgtggcgc gggccgcgca gcaggctgcc aagcgcggcg agcagcgccg
     9481 ctcccgccgt gacccacaag ccgttgtgca cggcggccac cacatccgcc gctacgggcg
     9541 tggacgtgtg gccagcgcgg gtcatgtaga tcaggcccgc catcgcgatc cccgacgcaa
     9601 agccggtggt gcgcgccgcg gcgagcattg cgcccgccac tccgtggcgt gcggcgggcg
     9661 ccgagcccat gatggcgctg ttgttcggcg cagtgaaaag ccccgccccc agccccgtca
     9721 gcgccagaaa caccgccgcc agcgcgggcg actccacgcc aaaaccggcg aggcacgcat
     9781 taccggtagc caccagcagc atgcccaacg tggctggcag cgtcacgcca accttgtccg
     9841 agacatagcc gctgagcgca gccaccggta ccatgcccac cggcaccgcc atcatccaaa
     9901 cgcccacgcg cgccggctcc cagcccgcgc ccagcagaaa ccgaaacggc atcagaaacg
     9961 acagcgaggc cgcgcagaca tagctgagcc acgccgccgc catcgaggcc gcgaagcgcc
    10021 ggttacggaa cagcttgagg tccaacgccg gataccggca acgctgctcg caaatcaaca
    10081 gtgccaccaa cgccagcagg cccgtcagcg cggcagtgat aatcaaggga tggcgccaac
    10141 ccatttcgcc gcccttgctc aacgcgaaca gcgtggccgc aagtccgatg gccatggccc
    10201 ctgctccaat cggatcgaag ggttggccgg cgccgttacg gtccggatgc agcaccacca
    10261 gcgccagccc ccacatggcc aacccaattg gcacgttgat ggaaaaaatc gtgggccagc
    10321 caaagtgctg cgtcagaaac ccgcccaacg gcggccccaa cgccaggccg agatacgtca
    10381 gcgtggcttg cattccaagc gcccgcccgc gttccgacga cggaaacgcc gccaccagca
    10441 gcgccgggct cagcgcgaac agcatcgccg cgcccagtgc ctgcaaggcg cgcgacgcaa
    10501 taagcaaccc gatggtacgc gacaggctgc acgccagtga gcccagcgtg aaaatgacaa
    10561 tgcccatgag gtaaatgcgc cgcttgccgt ggatatcggc cagtcggccg aacgccagca
    10621 acgacgagct gaccacgatc aaataggcca acatcaccca ctccagcgag gccaccgttg
    10681 cgcctgtttc gcggctgatg accggcaacg ccacattgac cacgctcgta tccagtgccg
    10741 acatgaaggt gcccgagccc accacggcca aagtccacca cttgcttgtc agcgtcgcgt
    10801 gtttcattgg atcaggtctt tagccgcggc ggcgttgcgt ccaaatgccg gtggtcagca
    10861 agaccgcggc cagcaggttc atggcgccta gcagcagggc aatctggagc ggatgctgat
    10921 ggaatagcgc gaccccgccc acgtaaaaca ggccgcaggg caccaacgcg tagcaccagc
    10981 ggaagcggcg ttgcgccatc tcgaaattga gcatcagata ggccaacgcc agcgggcaca
    11041 tggccagcac catgccgcga gtcagttgtg cggcaaacgc ggcatcttcc gcggtccagc
    11101 ggccgtagag aatggtccac ggcagcgcgg gccacagcag gcacacaccc accaccgccg
    11161 caatcagtaa accggaaaac gccaacccgc gcagcagaag ccgccaactc acgtcggtca
    11221 gttcgccggt ggaggtcact ttggggaaca acgcggcggc caccggcacc ggcaaaaata
    11281 cggcggtacg cgcgatggtg gccgccttgg caaatagccc ggcgacttcc tcgtcgaagt
    11341 agagcttggc caacgtggtg tcgagattca tcagcatggc gtagccggcg aggcacacca
    11401 ccgccgaacc gagatagcgg taggtctcgg cggggcgcac gtggccggcg ccgcgcggca
    11461 ggcgcatcaa caacgttgcc agcaggcaca gcgccaacac ggagagcaca ccaaacccct
    11521 gcgccgccaa cgccgcgaac gccgtcgcgg aaatccaata ggcgaagatc acgcccagca
    11581 ccaaccgcac cacaccccac gcctgcggcg cccaagccag ccacacgaac cactgccggc
    11641 cttgcagcag cccaaggaaa agatacatcc acaagctggc cgccaagacg gcgaatgtca
    11701 gcacaatcag ccccggcgaa acctgccaga ccgcggccaa ctttccgcgg cccaaccacg
    11761 ccgccgtaat cagcacaact gtcagcgccg tgaaaatctt ggcccaatga aaaaagagcg
    11821 ggcgaatctc gctggcgcgg ttttcctgca caaagtgcga gacaaagtgc gccagcgtgt
    11881 tttgcaacgc cagcatgggc gtggccacca ccatgatcag ccccagcatg gcgatgaacg
    11941 cgccatattc cgcgttgggc agcaggcggc ccagcgccat gtgcagcacc gcgttgcaca
    12001 gcgcgccgag gaaggtggcc accaccagca acaagccgtg gcgcaccagc ccgccttcgt
    12061 catgcttggc ccagttccac ttcatcgcgc atccggcggc aagtcaatca cccggcccag
    12121 cacccggtcg tagagggtca ccacccagct cagcggcgaa tacagcaggc gcaggcggca
    12181 aatggccaaa aacatttggg cggagacccg tcccacgcgc agcttggagc cggccacatc
    12241 gctccacgtg gtggggattt ccgtgatggg atagcgcgca cggcgggtct ggaacaacat
    12301 gtccacatcg aacgcccatt tggtgaggcc gatccggggc acgatgtcgc gccacgcgac
    12361 ggcggtcatg agcttggcgc cacattgggt gtcggtgatt tgcaatttga acaggcggcg
    12421 caccagccag ttgaaaatcc gtgatgccac acggcgcggc cacggttgcc gcggactgac
    12481 caccgcgccc ggcagccgcc gcgaggcaat aatcaggccg gcctggccga tgttgtccac
    12541 caaatcctga aacgcagcgg ggggcgtcgc gccgtcggca tccacaaagc cgatgtagtc
    12601 accgcgcgcc tgccggccgg ctgccataat cgcgccgcct ttgccgatgg gccgnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccac aattccagcg tggtgcccgc
    12781 ctgcccgaag ccggggatgg attccacgtg ctcatagcgg gcggccaaaa tgtcggcgaa
    12841 ggcctgctga tcctcgcggg tgacctcttc cacttccggc gaacggatgg cgaggctgta
    12901 gccgctgaat gcggcaatcg gcgcgttggt ctgcgtggcc agcaattcca gcatcatgtc
    12961 gcggttcagc acaccgattt tctccgcctt ttcgcgcggc caatcggggt agtagctgaa
    13021 cggccccatc tccatgccat gcggcacgtc caggccggtt tccactgcca gatacgtgtc
    13081 ctgcgtcaac agctccgtgg caccggtggg cttgttcaga tcgcggatcc attgggcggt
    13141 atcgcgcaac tggcccagcg ggctttggga gcgcagccgc caccagatgc ggtcgcgccc
    13201 cgccacaaac cagctttggg tttgcgggga ggaaaacgcg tagaggctgc acagcagcca
    13261 cgccaccacg aggatggcca accgccgcag gccgcgccat ggcggcgtct cgccgaagcg
    13321 ccggccttcc tcgcgcagcc accatcgcgc ggcggtggcc gtcagcgccg cgcaatacac
    13381 cgggtagatc ggcacctgat aatcgtcata gggaaacggt gcggccaaat gcaccagcgc
    13441 cagcgcaaac accgtcaccc acaaaaacgt gttgaagcac ggcgcgatgt cttccggccc
    13501 ggcgtagagc ggcgaatcct cgccgcggtc acagggcgat ttgccccgca gcaccacgta
    13561 gcgcccggct agcatcagca gcgccagccc cgccgccgcc gccgggaaat acgcctgccc
    13621 cagccgcgag aggcaacccg ccttgtaggc cagttgcgtc agccacgggc ccgattcgcg
    13681 cagcgtgtga tattccaaca cgccgaattg aaatccttcg cgccccagca tcatgaaggg
    13741 caggaacgtc accgccaagc caagcaaccc gcccagcgca aaatccagcc atgcccacag
    13801 gcgcaatcgg cggcggcaaa tgaaaaggta gagcccgccc accgccagcg ccgcccccag
    13861 cgagatgcgc gtggccgtgg cgcacgccag caaaaagccc gccaacgtgg cggcccggaa
    13921 gccgcgtcgc ggccccacat aggccagcgc cagtagcccg gccgacaaaa acaaactggc
    13981 cagcgaatag gttttgacca ccgtggtgaa atacgattgg tacacattga ccgcgatcaa
    14041 aataaagcac agcccccccg tgaagcgcag cgcccgccgc ggcccggccc gcatggccag
    14101 ccacaccgcg aacaacgtgc ccagcgtccc aaagccccac gtcactgcgc gcccgcccaa
    14161 cagcccatat tttcccaccc agccatacac caacgaatag accagcggca gcatgggcgc
    14221 ctgcgtgaag gcaaaatcgc ggtagggcca tagcccttcc cccatctgct tggcggcata
    14281 gaggtaccag ccttcgtcct ggttcagttc ccccatccac agattcaggc cggccagcgc
    14341 caaccatgtg gccaccaccg ccaaaaacaa cgccagatgc ccccaatgcc aatccgggac
    14401 atcctgcgcc cgcacgtttt gttcagcttc cataatgccg cgcgattctc cccaaccccc
    14461 ccgccctccg caaccccaaa aaacggatgc cccgcggggc gcccgcggcg cgctcgccac
    14521 ttatccgcgg gagaacagcg gcgaccaccg gcgcaccacg cgcatcgcca catccaccag
    14581 caccactgct gccagcgcca gcagcaccgc gctcaagccg ccgatcaact tgctgccatc
    14641 ctgctggaag ttacgccaca caatctgcgc cagcgccagc atcgtggtca cgaacatgaa
    14701 caccatcggc aaccatacaa accacgccga cttgccttgg ttgcgcagcc agccatacac
    14761 catcagcagc gccattgcgc ccatcaactg gtttgtcgcg ccaaacgccg gccagatggc
    14821 gcgccacgcc ggcatcaact gcccccccgc ccccgggatt tcctgaaaca ccacataggc
    14881 cggcagcgcc agcaccacca ctgtcgaaac cacccgcttc caggtggtct ctgacccaaa
    14941 gcccgtcagc tcctgcaaaa taatccggca caatcgtgtg cacgtatcca acgttgtcag
    15001 tacaaacgtg ctcaccgcca gcatcgccag cgacatgatc caaccgtccg gcacgcccag
    15061 cggtcggaag aaccgctcga tccccgccgc gaaaatagcc ggtggcgaca gcgccggctt
    15121 ctccgccaaa atcattactg tcgccagcgc cagcaccgcc agtacggctt cgaccagcat
    15181 ggcccccagc cccacccgct tcacggaact ttcccggttc aactgcttgg acgtggtccc
    15241 gctggccagc accgcgtgga agccgcttac cgcgccgcag gcaatcatca cgcagagcgc
    15301 cggataaatc cacccatccg ccggcgtctg cgccgtgatg aacgccggcc aggcaatcgt
    15361 cgccgcgccg ctccccgacc ccagcaacaa ccccaccgcg ccccccagca ggcaggcgta
    15421 aagtaaaaac gacgacaaat aatcgcgcgg ctgcaagagc gcccataccg gcaacgccga
    15481 cgccaccaaa caatacacca gcaaaatggc cgaccaaaaa tatttgtccg accccaacag
    15541 cgagggcacc aacgatggcg ccagcggcca cacctgcccc agccacaacc cgccaaacac
    15601 cagcggcacc gccagcagcg tggctttgcg cagcggcatc ttccagcggt tcagcaccca
    15661 cccaaacacc gccgccagaa aaatataaaa caccgatgcc gtcgccacca cgccaccaat
    15721 ccgcgactcc accgtttccg gcgcccccca cttcagcgta atcggcgcga acgtcgccgc
    15781 agtcatgtcc agaaacacca tgatcacata aagcagcacc agcaggatga acaatagaaa
    15841 caggcgatac gttagcgggc ccatcgcgct ccggccgatt tgcgccaagc tctgcccttt
    15901 gttgcgcacg ctggccatca tcgaggtgta gtcctgcacc ccgccgataa agatcacccc
    15961 cagcacaatc cacaccagcg ctggccccca gccaaacgcc aacgccgccg caatcggccc
    16021 cacaatcggc cccgcaccgg caatcgagct gaaatgatgc ccgaacacca ccgcatccgg
    16081 cgccggggca taatccaccc cgtcatacat cgtgtgggcg ggcgttagcc gttcctccag
    16141 catgtccagc cggcgctcca tccagccgcc gtaaatctta tacgccgccc agaacagccc
    16201 gcagcaaccc agaaatatca agcccagcat acgtcaccac cgttttgaaa gggggccatc
    16261 ttctgcctgc cttgacgaaa aatccagctt tttgcccaac aatttttcag cgcgcggtca
    16321 ccgcggcgca aggttttcca caccgtggaa aaaatccgca taagttttcc atagcgtgga
    16381 aaaatgggcc caaagttgtt ccattgtgtg gaaaaatttg tgaaagtttt tccattgcgt
    16441 agaaaaatac ccgcgctgcg acccgaattt ttccacactg tggaaattat ttttccacac
    16501 tgtggaaaac catcgaatca agcaggcaaa ggcaacacgg ggcgtgattc atatggtaga
    16561 gaaactgggt cggcagccgt gaatcacggc tggtccctga accctgacct ctgacctctg
    16621 aaccctcttt cccacgctgt ggaaaaccgg ctgtcgctag gccttgggcg ggggtgtggg
    16681 cgcgaggcgg ccggtttctt ccagctcgcg gaaggcggtg atgctgtcga acgcatcctt
    16741 gcagtagcgc accggcccgg aataagcggg ctggcagtca ttgatcacga agttttccgt
    16801 gagcgccgcg ccgcccagca gtaccgggat gttcagcccg gcggcttcca ttgccttgag
    16861 gttatcgcgc atgacctgca ccgatttgac gagcaggccg ctcatgccca gcatgtccgc
    16921 cttggtttca cgcacgactt ccaacattcg ctcgatggaa accttgacgc ccaggttcac
    16981 cactttgtag ccgttgttcg agaggatgat atccaccagg tttttgccga tgtcgtggac
    17041 gtcgcccgcg acggtgccaa tgacgagggt gccggcggct ttggcctcgc ccgccttcag
    17101 gaaaggcttg atcatgtcca cagccttctt catgacctcg gctgacttca gcacaaacgg
    17161 caactggagg atgccgtcgt tgaacaggcg gcccacttcc ttcatgccgg ggatcaggtg
    17221 gttgttcagc acatcttccg cggccatttc cgccagcagg gctggaacgg aatcggctag
    17281 tagcgcggcc ttgcctttga ccagtgcggc ctgcacctgt tcggcgggag tttgtagcgc
    17341 ggcctccgcg gcggcttcct cttccgggct ggtggtctcg aagtgctgga tatagacctc
    17401 gagcgggtcg ccttgcgcgg catcgttgtc cagcaggcgc tcggcgaggg tcaccgcggc
    17461 gggtgccagg tccgtcagcg ccaccaaggc ggaagtattg atgatgcacg catccatgcc
    17521 ggccttgacg cagcggtcca ggaaaaccgt gttgagcact ttgcgcgcgg ccggtttcaa
    17581 gccgaacgaa atgttcgaga gccccagcag cgtgcgcacc ccggggattt cctgcttgat
    17641 gcggcggatg gcttcgatgg tctccacggc ggcggttttc agcgcggggt caccgctgcc
    17701 gatggtgaag gttagcgggt caatgagcaa atcgccgggg cgcattccgc gctgaacaca
    17761 aaagtccacc agccggcgcg cgatttccac cttacggtcg gcggtcatgc ccatgcccac
    17821 ttcgtcaatg gtcagggcca ccagcccagc gccgaattgc ttggccagcg ccacgagttt
    17881 ttccgctttt tcttcgccgg attcaaagtt gatggaattg atcaacattc gcccgccgtg
    17941 caatttcagc gcggcttcca ccacgtccaa ctgcgtgcta tccaccatga tcggcagccg
    18001 gcactcgcgg gcggcccgcg ccaccagcgc ggtcatgtcg gcggtttcgt tgcgcccggc
    18061 ataggccacg ctcaaatcca gaacgtgcgc cacgccttca tcctgctcca ccagcaaatc
    18121 gaatgcggtg tcgagctttt cggccagcag ggcttcgcgg aactttttgg agccggtggc
    18181 attggcgcgc tcacccacgt acatcggcgg cgggttttgg gtgaggtcca tcggcgcgaa
    18241 caggctggcc acctgctctg gcggttgaac gtcgcgtggc gcgggcgcgg gcgcggcgcc
    18301 aagcgcgcgg cgcaattcac ggatgtgttc cggggtcgtg ccgcagcaac cgccagctac
    18361 gttgagcccc agccgttgca ccatcggcgc aaatatccgc gcgaagtcgg ccgcgcccag
    18421 cggataatgc acgcgcccct cttgcagcgt gggcaggccg gcattgggca tgcaccccag
    18481 gccaccgcgc cattgccggg cgatggcctc caagtggcgc tccatggctt ccggcccggt
    18541 ggcgcaattc aatcccaaca catcaatcgg atatggttcc agggcggcca tgaccgctcc
    18601 gatggacgac cccactagta gcgtgcccgt ggtttccacc gtcacggaaa cgtaaagtaa
    18661 tttgtcggtg ttggcgccca tcactttttg ggccgctgcc accgccgcct tgatttgcag
    18721 caagtcctgg caggtttcaa tgatcagccc atccgccccg ccctccaaca ggccggtcat
    18781 ttgctcttgg tagccgccgt agagttcgtc gtaggtcacc tggccgagcg tcaccagctt
    18841 ggtgccggga ccgacggagc ccagcacata gcgcgggcgg ccgctctgtt gcgctgcctc
    18901 attcgcggca ccgcgggcaa tttgcgcggc ggcgcgggcg atctcccgcg cgcgggcagt
    18961 caattgatat tctcccaacg tcagcggcga cgcgccaaaa ctgttggttt ccactgcatc
    19021 gcaacccgcc gcaaaatagg ccgcgtggat gtcacggatg atttccggcg cggacagatt
    19081 cagccactcg ttgcagccct cataggcgcc ccaggccgcc gcgcccggat tgcgattctg
    19141 gatttgcgta cccatggccc catccaccag cagcaggttg tggttcagaa attctttgat
    19201 gcgcatcacg ggctccgttt gggttgttgt ggaatgggca atctactgcc ccggcgcgga
    19261 ttcctcaacg aaagatttgg cgtttcccgc cgcgcgaatc atgggctggc cgggagctgc
    19321 gtgcgccatt tggtgatcca gtagggctcc gcccgccaac agcgcatggc ctcgcgcagc
    19381 atggcttggg caaaattggg cgctttgacg atcaggacgt tttcatcgtt tttgtcaaac
    19441 gccgcctgcg agaagttggc ggagccgagc acgacggtct gccggtcaat cacaatgact
    19501 ttgtgatgca ggagaacccc cggtgacatc cggacgttgg cccccatggc ctccagttgc
    19561 ctggtgttgg cgctggggtg agtggccatg ccggaatcga gcacaataat ggtgcgcacc
    19621 ccgcggccga ttgcgcgctt gaccgcttca gtcatcgcgg catccgtgaa cgaaaacgcc
    19681 aggatggcaa tctcgttggt ggccttgtcc atctccgcca cgatgccccc gcgggtgtcg
    19741 tcttcgggcg tgaaatagtt gtggatcaat accccgccga cattcacttc ggggtgcggc
    19801 gtgggatatg cgaagcgctt gccgtggcgc ttttcaaaaa tttcgagcca ctccagcgaa
    19861 tagttttcgg ccagctcctt gctttccagc acgatggcgt ggttgtcgtt gaaccgcgaa
    19921 cagttgcgcg tgaggttgta ggatccggtc cagaccagcc gtttatcggc caccatgaac
    19981 ttgttgtgca tcaggcccga gcggttgtgc gccagcgtca gcatgcccag cttttccatc
    20041 tgcgccagtt gcccggaaat ggcgtaggcc ttctccgcgt tttcctgggc cacaatcacc
    20101 cgcaccttga ccccgcgcgc gcgggcatcc aagagcgcct gcgcaaactc cggcagattc
    20161 aggtcataca cgcagacatc cagcgtctgc gtcgccgcgc cgatgaacgc caccaccgct
    20221 ttttccatgg tgggcgacgg ccccaaaaag aaatataccg gcacatcgcc gaatttgccc
    20281 ttgagcagcg tatccgcgta aaacggatcc acatcaattt gaaacagact ggccgcgccc
    20341 ggcaaaacgg ccgccgccag caccgccagc attaatccaa tgactcggaa ccttctgagc
    20401 atttggttaa agtaccacag tttgcactca aacgaaaacc acaacatcgc cgcgcgcgcc
    20461 gttggcagtg gcgacaaacg gccagcggtg aagttatccg tggctcacaa cgcgatggcc
    20521 gggcgaaggg tgccggagac atcggccacg cagggaatcc acgcgagacc aatttgctgg
    20581 atgctggtat ccgcgcgtcg cggggcaacg cggacggtgg acactttcag gttggctgga
    20641 tcgaacgccg cacccgtggc cgccatttcc tgctccagct cggcttccaa tgcggcgcgc
    20701 tgctggcgga tggcctccag cgtttcgccg gcttgggcaa catccagttt ctccttgctg
    20761 gtgcggctca tggaattgat gccgctggtg gcccgcccca ccccgcccat gcgtcggccc
    20821 aacagcaccg agagcgccgc cgcgccaaaa ttgaccatgg tgcccatgcg ttgccgcgag
    20881 agctgcgctt tttcgttttc cagcttggcc accgcgcggc gttcacgctc cgcgagcgtc
    20941 gtcagtttct gggcatattt ctggcgcatt ttttgcatgg cctcatcgcg cttttcgcgc
    21001 agaagctgat tggctcgaat gcgaaagtcg ccttcggttt cgcccggctg cgaggtcagt
    21061 ttatgttctg gcaacgtgaa cagctcgcga gcgaaagcct ccgcaagaaa tgctttgtaa
    21121 tctttttcgt gctgggcata gcgcttgggg tcggcaaggt ccgtgggcac gttctcatgc
    21181 gccgcgccgg gtagcgggtc gcggtcatcg ccggcggggg cgttgctgac tgcggtccac
    21241 accaggtggc cgtgctttgg atcggtccag acgagatggc ggaccgtttc ccacgaatcg
    21301 aggccgtatt tcagatcggc aaagtggatg cgggcgctgg ccatgagcgc cgggcggtac
    21361 gtgatggccg cgccggccgg cgcttgggtg gccggccaat aacgctgcgg gatgctggcg
    21421 gcgaggacgg ttttgggcgc gggggcgggc gcggcaggca tggcgactgg cgcggcgggc
    21481 gcgaccgcgg gcacaacgct cggcgcggcg ggcggtgcac tttgctgctg gggcatcagt
    21541 ttgctgatct gcgcgggcgc caatggcccc cgcaggtacg acaggcacca gcgcgtctca
    21601 aacagcaccg gcgcgtcatc gtgaacattc cgcatcagga aaatccggct gccgagattg
    21661 gccatcagcc ggtccagcgt ggcacgatcc aagccctcgc cggcagtggc gctggtcagt
    21721 ccttcaatga cgcgcatttt gtcgcgctcg gtttgcaggc ggccaatgag ccacgtgccg
    21781 gtgttggcaa ggccgcggta atccaagtcc acgggattct gcgtggccag cacgatgccc
    21841 agcccgaatg cgcgggcttg cttgagcagc gtgagcatgg gggccttggc gggcggattg
    21901 ctggtggggg ggaagtagcc aaagatttcg tccatataca gcagcgcacg cagcgtggag
    21961 gtgccggttt gccgc
//
LOCUS       DAOPNP010000032        21877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_2982_length_21877_cov_8.366603, whole genome shotgun sequence.
ACCESSION   DAOPNP010000032 DAOPNP010000000
VERSION     DAOPNP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21877
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2982_length_21877_cov_8.366603"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..452)
                     /locus_tag="PKI64_04335"
     CDS             complement(51..452)
                     /locus_tag="PKI64_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00273.1"
                     /translation="MAKSTTRKTQPAAERARRIELRFLEAVRARLPEHPGVVESLGCL
                     YTEMGRYHDGLAADRQLVKMEPASPLAHYNLACSLALTGQPDEAFAELEQAIALGYDD
                     ADWMASDEDLISLREDPRFGRLLARMQPSAS"
     gene            complement(461..1765)
                     /locus_tag="PKI64_04340"
     CDS             complement(461..1765)
                     /locus_tag="PKI64_04340"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOE00274.1"
                     /translation="MSNKTQQPQATLTIDGKNVELEMLTSTMGERALNLSGLKAKTGC
                     FAYDPATVNTAVCRSAITYVDGDKGILLHRGYPIEDLADNCSFIEVAYLLIHGKLPTE
                     SQRQKFSGLLNIHSMLHEDMRSFFGNYPEGAHPMAVLSAMVVSLSSFYPELREDSGQE
                     EIDITVTRLLSKLRTIAAFSYKKSIGEPYVYPSHKYSYCENFLNMMFRSPVSQYEADA
                     YDVAAMNKLLILHSDHEQNASTTAVRMVGSTGANLYACISAAICALWGPLHGGANQAV
                     IEMLEGIHRDRLTIDQVLAKAKDRNSKFRLMGFGHRIYKSYDPRARLTKKLCMEMAAK
                     RKINDPLVEIALELETRALADDYFQTRNLYPNVDFYTGLTYRMMGFPKEMFTVLFALG
                     RLPGWIAHWQEMRADSEQKIMRPRQIYVGPQRTEFVRIEARK"
     gene            complement(1885..2112)
                     /gene="rpmB"
                     /locus_tag="PKI64_04345"
     CDS             complement(1885..2112)
                     /gene="rpmB"
                     /locus_tag="PKI64_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOE00275.1"
                     /translation="MSAICEICGKGPSSGSRIVRHGLSKKKGGIGLHTTAVTKRRFKP
                     NLQKLRAKVGGGVKTMTVCASCIKAGKITKA"
     gene            complement(2257..2844)
                     /locus_tag="PKI64_04350"
     CDS             complement(2257..2844)
                     /locus_tag="PKI64_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOE00276.1"
                     /translation="MRDILVHACCAHCLGKLLAALRAGPEAWAPTIFWYNPNIHPLIE
                     FRRRLKAVKMLVERAALPLIADETYGLVDFCRAVHGHETAPERCRECYALRLGRTAQQ
                     ARAAGCAVFTSTLVTSRHQDHELIRAAGEAAGAAASVEFLYLDARAAEADPRLVHMLY
                     HQQYCGCVFSEYDRYKGTTAHLWPPPPAPTQTNPA"
     gene            complement(2850..3413)
                     /locus_tag="PKI64_04355"
     CDS             complement(2850..3413)
                     /locus_tag="PKI64_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOE00277.1"
                     /translation="MKKKNVCVLVADGSEEMEVVIVVDVLRRAGVSVFLAGVGEETRM
                     VTTSRGVRIAPDGAWDPAEANRFDALVIPGGIGGTEAMCADASVKQAVRDFVAAGKWV
                     ASICAGPTVLRDAGVLTGRRFTCHPACREDLQSAGTWVDERVVRDGNIVTSQAPGTAF
                     AFALALVELLESRATAQNVAAGMLYPL"
     gene            complement(3435..4460)
                     /locus_tag="PKI64_04360"
     CDS             complement(3435..4460)
                     /locus_tag="PKI64_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOE00278.1"
                     /translation="MAGKRPVQLSAVVSSDNAADSATSVAQDPAVAQFVQHLRAERNA
                     SEHTVAGYLSDIAQFCRQLWGEETAPPFPWKTPDRFSARRFLASFQKAGMAPTTASRK
                     MSSLRSFFRFLVREGMVKDNPFAGLQQPRLRRRLPQVLTRDEVLRLLAAPAQVQVNEK
                     KRSRPASAFAAYAVQRDTAILELFYSTGMRIAELCGLTTARLDLLSGTALVRGKGKKE
                     RFCPIGRPAAQALQAALDARDHWLAERNFRRPSAVFVSSKGGPLTPRSVERSFKKYLA
                     VAGLNPAITPHVLRHSFATHLLDAGADLRSVQELLGHASLSTTQIYTHVSVERLKEEY
                     DKAHPRA"
     gene            complement(4491..7058)
                     /gene="topA"
                     /locus_tag="PKI64_04365"
     CDS             complement(4491..7058)
                     /gene="topA"
                     /locus_tag="PKI64_04365"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOE00279.1"
                     /translation="MAKSLVIVESPAKAKTINKLLGGDYIVKASLGHVRDLPERALGV
                     DVQRGFTPQYVPIKGRAKVLADLQAAAAKVETIYLAPDPDREGEAIAWHLREALKGKV
                     GEHNFRRVTYNEITAPAIRKAFSEPTEINQMRVDSQQARRILDRVVGYKVSPMLWRRV
                     PGASSAGRVQSVALRLVCEREKAIRDFQPVPYWVIGVKAAKQVDPRAPFVAQLAKLND
                     AKADIKDEALALRLMAELQQRTLRVKDVLRKEVRKNAPPPFITSSLQQAASSALGFPP
                     ARTMRLAQKLYEGINLGHGTVSGLITYMRTDSFTIAQEAREQAREFIRREHGPEFLPE
                     TPNVFRNRSSAQEAHEAIRPTDPARTPESLKEVLERDDWRLYDLIWRRFMASQMAPAR
                     IAQRTAEIEAVGSGPDTFLFRATASEIVFPGFMRVSGLEQRKKEEAENGDNDEVASLP
                     PLDPGEALDVVEWLNDRKETQPPPRFTEASLVKVLEENGVGRPSTYAQIISTLLDRKY
                     VDREKRALVPTALGLKVCDFLISHLDALFDVQFTANMEELLDEIEEGKVAWTEMLTNF
                     YAKFQEWMGAARGPAGDLAAVEKLLAALSAVTTWQQPMTRGEKTVYSDEKFVESIRRQ
                     IAENKRPISRRQQDALARLLFRYQDQIPTAAAVAEELKLEAPAESNREGPPRQETIAK
                     LAMLENVTFDPPRKFGKKVYDDRDFVESLRGQVAGGKRLSTAQIAYLDKIMRKYAEQM
                     PDFATRAAELGLTAEAAAPADAHLDEILALFAAVQTWRPATSRKGRKWDDREFLESLK
                     AQYAQRKQLSFKQVSALKRLALNYAAQIPGYAELAPKLGLPEPRVAKKETAKAPPAE"
     gene            complement(7078..8232)
                     /gene="dprA"
                     /locus_tag="PKI64_04370"
     CDS             complement(7078..8232)
                     /gene="dprA"
                     /locus_tag="PKI64_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE00280.1"
                     /translation="MTPRAAYITLNQIENIGPVRVRAMVEALGSPEAVLSAPAAVLAT
                     VRGIGPKVAEGIVTQRATVSADREESAAAALGARILTPLDDEYPAPLKTIYDPPLCLY
                     VRGTLTKQDEQALAVVGTRRASHYGMAQADKLAYLAAKAGFTIVSGLARGIDTAAHLA
                     ALKAEGRTVAVLGGALDKLYPPENRELAERIAAHGAVISEFPLGREPDRTTFPYRNRI
                     VSGLSKGVLVVEAGLDSGAMNTAEQALEQGRSVLAVPGRVDLPGAKGPHRLIQNGARL
                     VEDLPDILREFEFLFPPDEQARLAQRHDARQRLTLTGPEHAVVRALWPEPELDFDVLI
                     RKTGLPSAQLMTLAMQLEMKKIIRRLPGRRLALMDDIRRWDLEPEAASPV"
     gene            complement(8254..8937)
                     /locus_tag="PKI64_04375"
     CDS             complement(8254..8937)
                     /locus_tag="PKI64_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00281.1"
                     /translation="MASVNEWIVREYLEALGFLVRQPRKYQVMARAKGAYEELDLLAV
                     NPALPQPGALSKERIWGAAELAQVSSAIIAIRGWHSEKFTAAILASLPDMYRFAEPEV
                     VRLAEADLGQPNPAKILCLADLPADPDQRAQALAFLRTRGIDGVILYRPMLLELIERI
                     DVKRNYEKSDLLQILRILKNYDLLKSGQLELFTPFRRRRATPATPPVPEQTIPLPQSL
                     EDESSALDD"
     gene            complement(8921..10012)
                     /gene="aroB"
                     /locus_tag="PKI64_04380"
     CDS             complement(8921..10012)
                     /gene="aroB"
                     /locus_tag="PKI64_04380"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOE00282.1"
                     /translation="MACKVQIDLGERSYPIWVGAGVLSHLPEAVNGLRGLVVTDAHVA
                     ACHGERIAALLGGNWPTVVLSPGETSKSAACWADLLEKIAQARLDRTGVLVALGGGVV
                     GDITGFAAATYMRGIRFVQVPTSLLAMVDSSVGGKTGINLDAGKNLAGAFHQPIAVCA
                     DTDTLATLPPREFAAGMAEVIKYGVALDAAFFAWLEAHTAAIQARQPAALERMVARCC
                     EIKADVVARDEREGGLRAVLNFGHTLGHALEKVTSYSGLLHGEAVALGMDFALGWSVA
                     HKGFPAADAARAIALLKQFELDPVRLRPANLDWQAVRAAMGRDKKAAAGAVRFVLCDR
                     LGHCGLPEPVSPATLDESLKGWCAHGIGQ"
     gene            complement(10088..10783)
                     /locus_tag="PKI64_04385"
     CDS             complement(10088..10783)
                     /locus_tag="PKI64_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOE00283.1"
                     /translation="MNLHPAHHPLLVGCIDSEELLRRCARQAPAVCDVLEVRLDLTGL
                     CGGAWLELCAAIEQQNKPVLLTIRSRREGGEWRGREAQRLALYLRALRVVSMLDVELA
                     ATGTVSMLVQAAKSEDIVLIGSFHNFRKTPPLPRLLAVAQRGRQLKVDVVKIATRVNT
                     SAELAILMALPALVKGPLSVMGMGPLGGLSRVAMAGAGSHMVYGSLAKATAPGQPPCH
                     ELAKELTRWGLRN"
     gene            10902..11252
                     /locus_tag="PKI64_04390"
     CDS             10902..11252
                     /locus_tag="PKI64_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00284.1"
                     /translation="MKRQPLRPALLLAAVASLALPILAEARPPQRHEPPRRPPACRPQ
                     PTKPPPRPPQHYRPPPPPCYFRPPPPVVYCPPPPPPPPPPCFCPPPPPPCYYGPTRTY
                     FYPAVPTVGFYFRL"
     gene            11318..11944
                     /locus_tag="PKI64_04395"
     CDS             11318..11944
                     /locus_tag="PKI64_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE00285.1"
                     /translation="MPSLLMTDTDNRQNDQAFVMDAVTRVVDGDVEAFAVIVHAYQRL
                     VAAELSRRLPAQDIQEVAQDVFVRAFRALPTYRHAAPFHIWLLRITRYAAMDFWRRKY
                     RRRTQLLGDLDEAGLRRVEQIQQQQVAEQETDAAAREAAREWLAAALARLAPDDRAII
                     TLVELEEHSMEEAARRLGCGVSAAKVRAFRARRRLHAVLTELRQQKHR"
     gene            11950..12285
                     /locus_tag="PKI64_04400"
     CDS             11950..12285
                     /locus_tag="PKI64_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00286.1"
                     /translation="MKTQSSTLWAQLRTAYRPEVPELDTAAIMAAVRQEARTPVRRTA
                     AGMAAAIPGWACTAAAVLAIFASGFVVGRAFNVADQHIRAAWTQSVQPEEFATTFLSY
                     EPARDDSSL"
     gene            12301..12729
                     /locus_tag="PKI64_04405"
     CDS             12301..12729
                     /locus_tag="PKI64_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00287.1"
                     /translation="MPKTKLTLLLLAALLLGACLGFYINDAIIRARIRSYSQMPENMP
                     QHITGLLTKRLDLTLEQQQQVLAVLNSYSDRMAQAREQTRNLFTSLLQEITAEVDQHL
                     TPEQVAEHKKMLDELQKRRQDNRALMRVYRPSESDNQSRK"
     gene            12768..13793
                     /locus_tag="PKI64_04410"
     CDS             12768..13793
                     /locus_tag="PKI64_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00288.1"
                     /translation="MKLHRSACLAVVLAALWLTAPLPATAAVPVAPILPTDYLGWTNA
                     YRLQNEQMEAVLVPAIGRLVHLALANGESPLRLEPALRGKTPDTNEPFFNIGGDWLWP
                     VAQARWPALAENGHDWPPPPLLADLPWECSAWTADDGQQCVQFSRTYGEPLNIVVTRQ
                     FTLAPTAAELIVHQRIERTAPSEIPVVLWNISQVAQPDQVLLPVATPSAFRGGLKTLM
                     GHKPDRRAMRRCGSAAIYTPAPGSEVKLGSDSPAAWVAARRGPVLIVESATTSATGNY
                     PDGGATVEIYSNHGLGYAEIETLSPEVALAPATVLENTLCIRLAATPEETGGCKLAEA
                     AQSLAAP"
     gene            complement(13833..14540)
                     /locus_tag="PKI64_04415"
     CDS             complement(13833..14540)
                     /locus_tag="PKI64_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00289.1"
                     /translation="MEKKFPQCGKVVGASPWRIGWQAARANLIPGLVLQAMAVALVAG
                     YYVSPRFHGWMQHVAEWQHRYGITFSILTRAVFNGVVPAVFCALVPGLRVRRPWAALL
                     FGVVWWGFMGANTHLFYTLQARVWGHDANFATVLLKTATDMLVYSPFYASPLVAVSHL
                     WQDLNYSWGATRLQLGPGWYRRIVLPNQVPGWTFWAPCLLVLYALPTPLQMPLAALLG
                     CFWALMCLQIALRTPAR"
     gene            complement(14553..15155)
                     /locus_tag="PKI64_04420"
     CDS             complement(14553..15155)
                     /locus_tag="PKI64_04420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE00290.1"
                     /translation="MVERSHFFEDLAHAEDDPLAFRAEHEPCLARLRARLGDVRGRRI
                     FEPGCGAGPLTVRLAEWVGGGGHVLALDASERMIARCAAAVAGLPQVTVRHGDITGYS
                     APAGSWDIVLVFRFYPHLPDPAAFLRECWDWLTPGGQLVVANLEGSRELNAMHAQLHA
                     VQHDHMPTAVALQQQLAAAGWRVAEAIDVPDDYFVRAWRD"
     gene            15278..15784
                     /locus_tag="PKI64_04425"
     CDS             15278..15784
                     /locus_tag="PKI64_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOE00291.1"
                     /translation="MKLGLLSDTHNHLLETRRALDLLLRHGAQHLVHCGDAGEDVVDL
                     LSATCQEHGLRAHVAIGNCDRMYGANSALYPQPTGITRGEYLEFVVAGKRCLVMHGDN
                     RRRLAQAIASGTLDYIFLGHTHEPHDERCGPTRLLNPGSVARPRRGPPTVLLLDLPTD
                     TPTWLPLA"
     gene            complement(15807..17072)
                     /locus_tag="PKI64_04430"
     CDS             complement(15807..17072)
                     /locus_tag="PKI64_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE00292.1"
                     /translation="MPRPKSSNPRSRVVNFRLTSDQYDEMEVCAKREGFSSLSGYLRN
                     LHSLVAAQHQKQEVVKEDSATYPLLLPTLFEETKHGKMYLGDSLGLLHNTLKPESVDL
                     VMTSPPFGLVRKKDYGNEDADQYVQWFRPFAAGFKRVLKKEGSLVIDIGSAWKAGLPT
                     KSLYHFELLLTLCKEYGFHLCQEHYWWNPAKLPTPAEWVNIRRIRVKDAINCIWWLSR
                     TPWPKASNRRVLAPYSDSMKSLLKNGYTPKLRPSGHDISDKFGVDNGGSVPPNLIALA
                     NTESNGGYQEYCRANSLPVHPARFPSGIPEYFIRMLTNPGDLVLDPFAGSCVTGEVAE
                     SLERKWICCELHEDYLKGARARFIGLKPRLRKQELSSYQIYPPCALNGTFDTTPLPSD
                     GGFKRKPKQTSNRLANKPLPFYAQQAAQG"
     gene            complement(17136..18131)
                     /locus_tag="PKI64_04435"
     CDS             complement(17136..18131)
                     /locus_tag="PKI64_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE00293.1"
                     /translation="MAALPPKEIVAAIIDAFTDTGAAATLISDVRSHPRRFYVVAGES
                     AFPLWVYIWTITHGGGAARPRNEYRIQLTSVTPPLPENPDGPTLLLGYEPNIKCFAGF
                     DLRKHKSFSVGSPSIQVNYKTLRDAIRDGFAYSRKGNDEIAIGFRPDNILAYALNAES
                     MHESGTDARVLTALEKITRLGPIDADETAVLPPERKRILAKVSRLVRDSDFRRKVTIA
                     YDRKCAVTGLQLRLIDAAHILPVGASGSTDEVTNGLCLSPTYHRAFDRGLIYLTEDCH
                     MVVNPKKKNDLVRLGLGGGLVEFEAHLGREIFLPADRRQWPNLAYIRAANQYRAS"
     gene            complement(18474..18746)
                     /locus_tag="PKI64_04440"
     CDS             complement(18474..18746)
                     /locus_tag="PKI64_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOE00294.1"
                     /translation="MKLTNDEKKLLDSAEKGEWKRIPDFLDQSSRYRESAQATLRKDK
                     RVNIRMTELDLVRLQKKAMQEGLPYQTLISSILHKFINGRLVEKMG"
     gene            complement(18748..19023)
                     /locus_tag="PKI64_04445"
     CDS             complement(18748..19023)
                     /locus_tag="PKI64_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015228540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOE00295.1"
                     /translation="MKPFDWNDEKNEWLRQERGITFEDIVFHLAHDGLLDILERPNPR
                     QYPGQRLFVVNVEGYACIVPFVKDDDKIFLKTIIPSRKMTKHYLGGD"
     gene            complement(19315..20232)
                     /locus_tag="PKI64_04450"
     CDS             complement(19315..20232)
                     /locus_tag="PKI64_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE00296.1"
                     /translation="MDQKVKNILEVAALPIIFWIIAVVLWQAKGSIFYLFNFGYIGTA
                     IGIGNGLFTFLPKRRRPIGRKITQLLVGLYMLVFLGLAKSENMQLEGFFFYVLMGMFA
                     AAALHYAIAKIVGPLVFNRGWCGWACWTTMVLNFLPYTKSPGRVARPWEWVRAIHFAG
                     SLILVLVLWFGFNYRPQHGSTELAWLLVGNGFYFLIGIVLAYALKDNRAFCKYVCPIT
                     VPLKIGARFSLLRITGDAEKCNECGVCTRNCPMDIQIPRYIKNGQRVLSTECIFCQNC
                     ISVCPNQALTYSFAFDVGGKEYLRRRPDR"
     gene            20551..21753
                     /locus_tag="PKI64_04455"
     CDS             20551..21753
                     /locus_tag="PKI64_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE00297.1"
                     /translation="MSINQKSFIRPQAAVLLERLREPRRFIQVVSGPRQVGKTTMVQQ
                     VVAQLTVPARYASADEPTLRGPTWIEQQWDAARLSATSAEGAVLILDEVQKVGNWSET
                     VKGLWDEDTRKRLPLQVVLLGSSPLLISKGLTESLTGRFENSILTHWSYAEMKKAFGT
                     SLNEYVFYGGYPGAASLIGKPDRWRNYLRNSLIETTIARDILLMTRIDKPALLRQLFE
                     LACRFSGQILSFNKMLGQLHDAGNTTTLAHYLNLLAAAGMVCGLPKYSGNVIRSRGSS
                     PKLQVLNTGLMNALGSASLPETHKQPARWGRLIESAVGAHLANSALRGDCAVYYWREG
                     IAEVDFVVQCNDRLTAIEVKSGHAPKMHKGTAALAKRFKLDRTLLIGGDGIAFEEFLS
                     EPVTHWVG"
BASE COUNT         4450 a         6905 c         6177 g         4345 t
ORIGIN      
        1 ggaacggtcg cagaccgacc agccattccc acccgacgaa agcctgtgct ctagctcgcg
       61 gagggctgca ttcgcgccag cagccggcca aagcggggat cttcgcgtag cgagatcaag
      121 tcttcatcgc tggccatcca gtcggcatcg tcatagccca atgcaatcgc ctgctccagt
      181 tcggcaaagg cttcgtcggg ctgcccggtc aacgcaaggc tgcaggccag gttgtagtgc
      241 gccaacggcg aggccggctc catcttgacg agttggcggt ccgccgccag tccatcgtga
      301 tagcgcccca tttccgtgta gaggcaaccc aaactttcca ccacgcccgg atgctccggc
      361 agccgcgccc gcaccgcttc cagaaagcgc agttcgatgc gtcgcgcgcg ctcggcggcc
      421 ggctgggttt tcctggtggt ggatttcgcc atggctccgc ttatttgcgc gcctcaatgc
      481 gcacaaactc cgtccgttgc gggcccacat agatttgccg gggccgcatg attttctgct
      541 cgctgtccgc gcgcatctcc tgccagtgcg caatccagcc gggcaaacgc cccagcgcaa
      601 agagcaccgt gaacatttcc ttcgggaagc ccatcatccg gtaggtcagg ccggtataga
      661 aatccacgtt gggataaaga ttgcgtgtct ggaaataatc atcggccagc gcccgcgtct
      721 ccaactccag cgcgatttcc accagcgggt cgttgatttt gcgtttggcg gccatctcca
      781 tgcagagctt tttggtcaac cgcgcgcgcg ggtcatagga cttataaatc cggtggccga
      841 accccatcag ccggaatttg ctgttgcggt ccttggcctt ggccagcact tggtcaatcg
      901 tcagcctgtc gcggtggatg ccctccaaca tttcaatcac ggcttggttg gcgccgccgt
      961 gcagcggacc ccacagcgcg caaatcgccg cagaaatgca ggcatacaga ttggcgcccg
     1021 tgctgccgac catccgcacg gccgtcgtcg aggcattctg ctcgtgatcg gaatgcagga
     1081 tcagcagctt gttcatggcc gccacgtcgt aggcatccgc ctcatactgc gataccggcg
     1141 agcggaacat catgttcagg aagttctcgc aatagctata cttgtggctg ggatacacat
     1201 acggctcgcc aatggacttt ttgtaggaaa acgccgcaat cgtgcgcagc ttggagagca
     1261 ggcgcgtgac agtgatgtca atttcctcct gcccgctgtc ctcccgtaac tcgggataaa
     1321 agctcgacag cgaaaccacc atcgccgaca gtaccgccat cggatgcgct ccctcgggat
     1381 agttgccgaa gaagctgcgc atatcctcgt gcagcatcga atggatgttc aacagtccgg
     1441 aaaacttttg ccgctggctt tccgtgggca gcttgccgtg aatcagcaaa taggccacct
     1501 cgatgaacga gcaattatcc gccaaatcct caatcgggta gccgcggtgc agcaggatgc
     1561 ccttgtcgcc gtccacatac gtgatcgcgc tgcggcagac cgcggtattc accgtggccg
     1621 ggtcataggc aaagcatccg gtctttgcct tcagcccgct cagattcagg gcgcgctcgc
     1681 ccatggtgct ggtcagcatc tccagttcca cgttcttgcc gtcaatcgtc aaggtagctt
     1741 gcggctgctg tgttttattg ctcattgctt gtgttccatt ccgtcgttcc ggtcccaaaa
     1801 agcaaaacac cacgtcgcgc gggcgcggcg gggtgtttca cgcaaggcag gcggtgctcc
     1861 gggcggagcg gccgccgcgc cggatcacgc tttggtgatt ttaccggcct tgatgcaact
     1921 ggcgcacacc gtcatggtct tgacgccgcc gcccaccttc gcccgcaatt tctgcaaatt
     1981 cggcttgaac cggcgcttcg tcaccgccgt cgtgtgtaac ccgatgccgc cctttttctt
     2041 ggacaggccg tgacgcacga tacgcgagcc gctcgacggc ccttttccac aaatctcaca
     2101 aatggctgac atgctcttct cctaagtcaa aagtgcgcga actataccca ccccctcccc
     2161 ccccgcgcaa gtgaaaaaga acccccggcc cgcgccaatt gttgtgcggt tcatcaggcg
     2221 gcatgcctac tttccgccca acgatccacg cctacattac gcaggattcg tttgcgttgg
     2281 cgccggcggc gggggccaga gatgcgcggt ggtgcctttg tagcggtcgt attcgctgaa
     2341 aacgcagcca cagtattgct gatggtagag catgtggacg aggcgggggt cggcctcggc
     2401 ggcgcgcgcg tccaaataga gaaactccac actggcggcg gccccggcgg cctcccccgc
     2461 cgcgcggatg agttcatgat cctggtgccg gctggtcacc aaggtggacg tgaagacggc
     2521 gcaacccgcc gcgcgggcct gttgcgcggt acgccccaag cgcagcgcat agcattcccg
     2581 gcagcgctcc ggcgccgttt cgtggccgtg caccgcgcgg caaaagtcca caaggccata
     2641 ggtttcgtcg gcaatcagcg gcagggccgc tcgttccacc agcattttga cggctttgag
     2701 tcgccgtcga aattcaatca atggatggat attcggattg taccaaaaga tggtcggcgc
     2761 ccacgcctcc ggcccggccc tgagagccgc gagcaacttg cccaagcaat gggcacagca
     2821 ggcgtgaacc agaatatcgc gcatgagaat cagagcggat aaagcatccc ggcggccacg
     2881 ttttgggcgg tggcccgcga ctccagcaac tccaccaacg ccagcgcaaa agcaaaggca
     2941 gtcccgggag cttggctggt cacgatgttg ccatcgcgca caacccgctc atccacccag
     3001 gtgccggcgc tttgcaagtc ttcgcggcag gcggggtgac aggtgaagcg ccggccggtc
     3061 aatacgccgg catcgcgcag caccgtcggg ccggcgcaga tggaggcaac ccacttgccc
     3121 gcggcgacaa aatcacgcac ggcctgtttg acggaagcat cggcgcacat ggcctcggtg
     3181 ccgccaatgc cgcccggaat aaccagcgcg tcgaagcggt tggcctcggc aggatcccat
     3241 gcgccatccg gcgcaatgcg tacgccacgc gaggtggtga ccatccgcgt ttcctccccc
     3301 accccggcca aaaacacgct cacgcccgcg cggcgcaaca cgtccaccac aatgaccact
     3361 tccatttcct cggaaccatc agccaccaat acacaaacat ttttcttttt cattttattc
     3421 tcctcttgtt gagcctacgc ccgcgggtgg gctttgtcat attcttcctt gagtcgttcc
     3481 acgctaacat gggtatagat ttgagtggtg gagaggctgg catggccgag caactcctgc
     3541 acgctgcgca aatcggcgcc ggcatccagc aaatgggtgg cgaagctatg gcgcaacaca
     3601 tgcggcgtaa tggcgggatt caggccagcc accgccagat attttttgaa tgagcgctcc
     3661 acggagcgcg gggtcagcgg cccgcccttc gagctgacaa aaaccgcgga gggacgacgg
     3721 aaatttcgtt cggctagcca gtgatcccgt gcgtccaacg cggcttgcag ggcttgggcg
     3781 gcgggccgac caatggggca aaagcgttct tttttgcctt tgccgcgcac gagtgcggtg
     3841 ccggagagca ggtcgagacg ggcggtggtc agcccgcaca gctccgcgat acgcatcccg
     3901 gtgctataaa acaattccag aatggccgtg tcccgctgca cggcataggc ggcgaaagcg
     3961 gacgccggac ggctccgttt tttttcgttc acctgaacct gcgcgggcgc ggccagcagg
     4021 cgcaagactt cgtcccgcgt tagtacctgg ggcaaacgac ggcgcaggcg gggctgctgc
     4081 aaaccggcaa aagggttatc cttgaccatc ccctcgcgca ccagaaaacg gaaaaacgag
     4141 cgcaagctgg acatcttgcg gctggcggtg gtgggcgcca tgccggcttt ctgaaacgac
     4201 gccaaaaaac gccgcgcgga aaaccggtcg ggcgttttcc acggaaatgg cggcgcggtc
     4261 tcctcacccc aaagctgccg gcaaaattgg gcgatgtcgg agagatagcc cgccacggta
     4321 tgctccgagg cattgcgttc cgcccgcaag tgctgcacaa actgcgccac tgccgggtcc
     4381 tgagccaccg aggttgctga gtccgcggcg ttatccgatg acaccactgc ggacaactgg
     4441 actgggcgtt tgccggccac taccctccca ctccttgccg cgccagccgg ttattcggcg
     4501 ggcggggcct tggcggtttc ctttttggcg acgcgtggtt cgggcaggcc aagtttgggc
     4561 gcgagctccg catagcccgg aatttgcgcg gcataattca aggcgaggcg tttcagcgcg
     4621 ctgacctgct tgaaggaaag ttgcttgcgc tgggcatact gggccttgag cgattccaga
     4681 aactcgcggt cgtcccattt gcgtcccttg cggctggtgg ccggacgcca tgtttggacg
     4741 gcggcgaaca gcgcgaggat ttcatccaaa tgagcatcgg caggcgcggc ggcttcggcg
     4801 gtcagcccca gctcggcggc gcgcgtggcg aagtcgggca tttgctcggc atacttgcgc
     4861 atgatcttgt ccagataagc gatttgcgcg gtggagagcc gtttgccacc ggcgacctgt
     4921 ccgcgcaggg actccacgaa atcgcggtcg tcatacacct ttttgccgaa cttgcgcggc
     4981 gggtcgaagg tcacgttttc cagcatggcg agcttggcaa tggtctcttg gcgcggcgga
     5041 ccttcgcgat tggattcggc tggggcttcc agcttcaatt cctcggccac ggcggccgcc
     5101 gtggggattt ggtcttgata gcggaacagc aggcgtgcca gcgcatcctg ctggcggcgg
     5161 gaaatcggcc gtttgttttc agcgatttgc cggcgaatgg attccacgaa tttttcatcg
     5221 ctatagacgg ttttctcgcc gcgcgtcatc ggctgctgcc acgtggtgac ggcggacaag
     5281 gccgccagca gtttttcgac cgcggccaaa tcgccggccg ggccacgcgc cgcgcccatc
     5341 cactcctgga atttggcata aaagttggtg agcatttccg tccaggccac cttgccctct
     5401 tcgatttcat cgagtagttc ttccatgttg gcggtgaatt gcacatcaaa gagcgcgtcg
     5461 aggtggctga tcaggaagtc gcacaccttg agaccgaggg cagtcggcac gagggcgcgt
     5521 ttttcgcgat ccacatattt gcggtcgagc agcgtggaaa taatctgggc ataggtgctg
     5581 gggcgaccca cgccattttc ttcgagcact ttcaccagcg aggcttcggt aaagcgcggt
     5641 ggcggctgag tttccttgcg gtcgttgagc cattcaacca catccagcgc ctcgccgggg
     5701 tcgagcggcg gcaggctggc gacttcgtcg ttgtcgccat tttccgcctc ttcttttttg
     5761 cgctgttcaa gaccggaaac ccgcatgaag ccgggaaaca cgatttctga agcggtggcg
     5821 cggaagagga aggtgtccgg cccggagccg acggcttcaa tttcggccgt gcgttgcgca
     5881 atgcgcgcgg gggccatctg gctggccatg aagcgccgcc aaatcaggtc atagagccgc
     5941 caatcgtcgc gttcgagaac ttccttgagg ctctccggtg tgcgggcggg atcggttggg
     6001 cggatggctt cgtgcgcctc ttgcgcgctg gagcggttgc ggaagacatt cggagtctcg
     6061 ggcagaaact ccgggccatg ctcgcggcga atgaactcgc gcgcctgctc gcgggcttcc
     6121 tgggcgatgg tgaacgaatc ggtgcgcatg taagttatca agcccgacac cgtgccgtgg
     6181 cccaggttga tgccttcata cagtttttgg gcgaggcgca tggtgcgcgc gggcgggaag
     6241 cccaaggcgc tgctggcagc ctgctgcagg ctgctggtga tgaacggcgg cggcgcgttt
     6301 ttgcgcactt ccttgcgcaa cacatccttc acgcgcagcg tgcgctgctg caattccgcc
     6361 atcaggcgca gggccagcgc ctcatccttg atgtcggcct tggcatcgtt gagcttggcc
     6421 aactgcgcca cgaacggtgc gcgcgggtcc acctgcttgg cggccttgac gccgatcacc
     6481 cagtacggca caggctggaa atcacggatg gccttttcac gctcgcaaac gaggcgcagg
     6541 gccacggact gcacgcggcc ggcgctcgac gcgcccggca cgcgccgcca aagcatgggg
     6601 ctgactttgt agcccactac gcggtcgaga atccgccgcg cctgctggct gtccacccgc
     6661 atctggttga tttcggtcgg ctcgctgaac gccttgcgga tggcgggggc ggtgatttcg
     6721 ttataggtga cgcgacggaa gttgtgttca cccaccttgc ccttgagcgc ctcgcgcagg
     6781 tgccaagcga tggcttcgcc ttcacggtcg gggtcgggcg ccagataaat ggtctccacc
     6841 ttggcggcgg ccgcttgcag gtctgccagc actttggcgc gccccttgat cggcacatat
     6901 tgcggggtga agccgcgctg cacatccacg cccagcgcgc gctcgggcaa atcccgcacg
     6961 tggcccagcg aggccttcac aatatagtcg ccgcccagca gcttgttgat ggttttggcc
     7021 ttggccggcg attccacaat tacgagactt tttgccatga gttctgcatc ctttccttca
     7081 aacgggtgaa gccgcctcgg gttccaaatc ccagcggcga atatcgtcca tcaaagccag
     7141 ccggcggccg ggcagccgcc ggatgatctt cttcatttcc aattgcatgg ccagcgtcat
     7201 cagttgcgcc gaaggcaggc cagtcttgcg aatcaagaca tcaaaatcca attccggttc
     7261 cggccacagc gcgcggacta cggcgtgttc ggggccggtc agggtgaggc gttgacgggc
     7321 atcatgccgc tgagcaaggc gggcctgttc atccggtggg aacaggaact cgaactcgcg
     7381 caggatatca ggcaaatcct cgaccagccg cgctccattt tgaatcaggc ggtgcgggcc
     7441 tttggcgccg ggcaaatcca cgcggccggg caccgccaac acgctgcgcc cctgctccag
     7501 cgcctgctcg gcagtgttca tcgcgccgct atccaacccc gcctccacca ccagcacgcc
     7561 tttcgacaag ccgctgacaa tccggttgcg atacggaaat gtggtgcggt ccggctcacg
     7621 ccccagcggg aactcgctga ttaccgcgcc gtgtgccgcg atgcgctctg ccagctcacg
     7681 gttttccggc ggatagagtt tatccaatgc gccacccagc acggccaccg tgcggccctc
     7741 ggccttgagc gcggccaaat gcgcggcggt gtcaatgccg cgggccaggc cgctcacaat
     7801 ggtgaacccg gctttggcgg ccagataggc caacttgtcc gcctgcgcca tgccatagtg
     7861 cgatgcgcgc cgcgtgccca ccaccgccaa cgcctgctcg tcctgtttgg tcaacgtgcc
     7921 gcgcacatac aggcacagcg gcggatcata aatggttttc aacggcgcag gatattcgtc
     7981 atccagcggc gtgaggatgc gcgcgcccag cgcggcggcg gccgattcct cgcggtcggc
     8041 ggatacagtc gcgcgctgcg tcacaatgcc ctcggccact ttggggccga tgccgcgcac
     8101 ggtggccagc acagcggccg gggcggacag caccgcttcc ggcgagccga gcgcctcgac
     8161 catcgcgcgc acgcgaaccg gaccgatgtt ctcgatctgg ttcaacgtaa tataggcggc
     8221 gcggggagtc atgggctggc ggtcagttgg gatttagtcg tccaaagcgc tgctttcgtc
     8281 ctcgagcgac tgcggcagcg gaatcgtttg ctccggcacc ggcggggtgg ccggagtggc
     8341 acggcgccgg cgaaacggcg tgaacaattc gagctggccg ctcttgagca aatcgtagtt
     8401 tttcaggatg cgcaaaattt gcagcaaatc agatttttcg tagttgcgct tgacgtcaat
     8461 gcgctcgatc agttccagca acatggggcg gtaaagaatc acaccatcaa tgccgcgcgt
     8521 gcgcaggaag gccagcgcct gcgcgcgttg gtcggggtcc gcgggcaaat cggccagaca
     8581 cagaatcttg gccgggttgg gctggccaag atcggcctcc gccaaccgca ccacttccgg
     8641 ctccgcgaac cggtacatat ccggcaggct ggccagaatg gccgcggtga acttctccga
     8701 gtgccagcca cgaatcgcga tgatggcgct ggaaacctgc gccagttccg ccgcgcccca
     8761 gatgcgctcc ttggagagcg cccccggctg cggcagcgcc ggattcaccg ccagcaaatc
     8821 caattcctca tacgcgccct tggcgcgcgc catcacctga tatttgcgcg gctggcgcac
     8881 caaaaagccc agcgcttcaa gatattcccg aacaatccat tcattgaccg atgccatgtg
     8941 cgcaccatcc tttcaacgat tcatccaagg ttgcgggaga aaccggctcc ggcaagccgc
     9001 agtggccgag gcggtcgcaa aggacaaagc gcacggcgcc ggcggcagcc tttttatcgc
     9061 ggcccatcgc cgcgcggaca gcctgccaat cgaggttggc ggggcgcaag cggaccgggt
     9121 ccaattcaaa ctgtttgagc aacgcgatgg cccgcgcggc atcggccgcc gggaagccct
     9181 tgtgtgccac cgaccacccc aaggcaaaat ccatgcccag cgcgacggcc tcgccatgca
     9241 gcaaaccgga ataggacgtc actttttcca gcgcgtggcc gagagtgtgg ccgaaattga
     9301 gcaccgcgcg caggccgccc tcgcgttcat cgcgcgcgac gacatcggct ttgatttcgc
     9361 agcagcgggc caccatgcgc tccaaggcgg ccggctggcg cgcctgaatg gccgcggtgt
     9421 gggcttcgag ccaggcgaaa aaggcggcat ccagtgccac gccatacttg atgacctcgg
     9481 ccatgcccgc ggcaaactcg cggggcggca aggtggccag cgtgtcggta tccgcgcaaa
     9541 cggcgatggg ctggtggaaa gcaccggcca agttcttgcc ggcatccaaa ttgatacccg
     9601 ttttgccgcc cacggagcta tccaccatgg ccagcaggct cgttggcacc tgaacaaaac
     9661 gaattccccg catgtaggtg gcagcagcaa aaccggtgat atcgcccacc acgcccccgc
     9721 ccagcgccac cagaaccccc gtccggtcga ggcgcgcctg cgcaattttt tccaacaaat
     9781 ccgcccagca cgccgccgat tttgaagttt cgcccggcga cagcaccacc gtgggccagt
     9841 ttcctcccaa caacgcggca atgcgctcgc cgtggcaggc ggccacatgg gcatcagtga
     9901 ccaccagccc gcgcagcccg ttgacggcct ccggcaggtg gctcagcacc cccgcgccca
     9961 cccaaatggg ataggagcgt tcgccaagat caatctgaac cttacaagcc ataacggggt
    10021 gaggaaatat ccccaacgcc cgccgttgtc aatgcgtttc ccgatgccgg cgcgggccag
    10081 cggcgggtta gttgcgcagg ccccagcgcg tcaattcctt ggccagttcg tggcacgggg
    10141 gctgccccgg ggcggtggct ttggccagcg agccatagac catgtgggaa cccgcaccgg
    10201 ccatggccac acgcgagagt ccccccagcg gccccatgcc catgacgctc agcgggcctt
    10261 tgaccaacgc cggcagcgcc atcagaatag ccagctccgc ggacgtattc actcgtgtgg
    10321 caattttcac cacatccacc ttcagttgcc gtccgcgctg cgccaccgcc agcaggcgcg
    10381 ggaggggcgg cgttttacgg aaattgtgga acgacccgat cagcacaata tcttccgatt
    10441 tggccgcctg caccagcatg gacaccgtgc cggtggccgc cagttccaca tccagcatgg
    10501 acactacgcg cagcgcgcgc aaatagagcg ccaaacgctg cgcctcgcgc ccgcgccatt
    10561 cccccccctc ccgccgggag cgaatggtca gcagcaccgg cttgttttgc tgctcgatgg
    10621 ccgcgcaaag ctccagccac gcgccgccgc aaagcccagt aaggtcaagg cgtacttcca
    10681 gcacatcgca gaccgcgggc gcttggcgcg cgcagcggcg caacaactct tccgaatcaa
    10741 tgcaaccaac aagcaacgga tgatgggcag ggtgcaaatt cataagcgca tcacaacaaa
    10801 ctccccggcg gaacaaaagt caagaatggc ggtgtaactt tcccaacacg gggggcgact
    10861 gtcatgggcg aagggaaaag atcatgaaag gaaccctcgc catgaaacgc caaccgctcc
    10921 gcccagcctt gctccttgcg gcagtcgcca gtctcgcgct cccgattctg gccgaggccc
    10981 gcccgccaca acgccacgag ccgccacgcc gccccccggc ctgccggccg caacccacca
    11041 agccgccgcc gcgcccgccg cagcactacc gacctccgcc gcccccttgc tacttccgcc
    11101 ccccgccgcc ggtggtgtat tgcccgcccc caccaccgcc ccccccgccc ccgtgcttct
    11161 gcccgccccc acccccgccg tgctactacg ggccaactcg aacctacttt tatcctgcgg
    11221 tgccgaccgt tggcttctac tttcggctgt aagctgactt gccgcaccgc ccgttctatg
    11281 aaatcctagt tgcggaatct ggaacctgcc gcgccgtgtg cccagcctgc tcatgaccga
    11341 caccgacaac cgccaaaacg accaagcgtt cgtcatggac gcggtgaccc gcgtcgtgga
    11401 cggcgatgtt gaggccttcg cggtgattgt ccatgcctac cagcggctgg tggcggcgga
    11461 gttgtcccgc cgtctgccgg cgcaggatat ccaggaagtg gcgcaggatg tgtttgttcg
    11521 cgcgtttcgt gccttgccaa cctaccgcca cgccgcgccc ttccacatct ggctgctgcg
    11581 gatcacccgc tacgccgcca tggatttctg gcggcgcaaa taccggcggc gcacccaact
    11641 gctcggcgat ttggacgaag ccggcctgcg ccgcgtggag caaatccagc agcagcaggt
    11701 ggccgagcag gaaaccgatg ccgcggcgcg cgaagccgcg cgggaatggc tggcggccgc
    11761 gctggcgcgg ctcgcgccgg atgaccgcgc catcatcacg ctggtggaat tggaagaaca
    11821 cagcatggag gaggccgccc ggcggctggg ctgcggcgtc tccgcggcca aggtgcgcgc
    11881 cttccgcgcg cgccgccgct tgcacgccgt gctgaccgaa ctgcgacaac agaaacacag
    11941 gtgacgaaaa tgaaaacaca atcttcaaca ttatgggctc agctccggac ggcctaccgc
    12001 ccggaggtgc ccgagctgga tactgccgcc atcatggcgg cagtccggca ggaggcgcgc
    12061 acgcccgtgc ggcgcaccgc cgccggcatg gccgctgcaa tccccggctg ggcctgcacg
    12121 gccgccgcgg tgctggccat tttcgcttcg gggtttgtcg tgggccgcgc gttcaacgtg
    12181 gccgatcaac atatccgcgc cgcgtggacg cagagcgtgc agccggagga attcgcgacg
    12241 acattcctct cctatgagcc cgcgcgcgac gattcaagcc tgtaagaaag ggagaccacc
    12301 atgcccaaaa ccaaactcac cctgctgctg ctggccgcgc tcctcctcgg cgcctgcctc
    12361 gggttttaca tcaacgacgc catcatccgc gcgcgcatcc ggtcctattc ccaaatgccg
    12421 gagaatatgc cgcagcacat caccggcctg ctcaccaagc gcctcgacct caccctcgaa
    12481 cagcagcagc aggtgctcgc cgtgctcaac agctatagcg accgcatggc acaggcccgc
    12541 gagcaaaccc gcaacttgtt cacctccctg cttcaggaaa tcactgccga agtggatcaa
    12601 cacctcaccc ccgaacaagt ggccgaacac aaaaagatgt tggatgaact ccaaaaacgt
    12661 cggcaggaca accgcgccct catgcgggtc tatcgcccgt cggaatccga caaccaatcg
    12721 cgcaagtagc gctggctttt cccgcgcgcc ctttttattc tgcccggatg aaactgcatc
    12781 gctccgcttg tttggcggtc gtcctggccg cgctctggct caccgccccc ctgcctgcca
    12841 ccgccgccgt accggtggcg cccatcctgc cgacggacta cctcggctgg accaacgcct
    12901 accggctgca aaacgagcag atggaagccg tcttggttcc ggccatcggc cggctcgtcc
    12961 atctggcatt ggccaacggc gaaagcccgc tgcgccttga acccgccttg cgcggcaaaa
    13021 ccccggacac caacgaacct tttttcaaca tcggcggcga ctggctctgg cccgtggcgc
    13081 aagcccgctg gcccgctctc gccgaaaatg gccacgactg gcccccgccc cccctcctcg
    13141 ccgatctgcc gtgggagtgc tctgcgtgga ccgccgatga tggtcaacaa tgcgtgcaat
    13201 tcagccgcac ctatggcgag ccgctgaata ttgtcgtcac tcgtcagttc actctggcgc
    13261 caaccgccgc tgaattgatc gtgcaccagc gcatcgaacg caccgctcct tcggagattc
    13321 ccgtggtgct gtggaacatc agccaagtgg cgcagccgga tcaggtgctg ctgccggtgg
    13381 ccacgccctc ggctttccgc ggcggcctca aaaccctcat gggccacaag cccgatcgcc
    13441 gcgccatgcg ccgctgcggc tctgccgcca tttatacgcc cgcccccggc agtgaagtca
    13501 aactgggttc cgattcgccc gccgcgtggg tggccgcgcg tcgaggcccg gtgctgattg
    13561 ttgaatccgc caccaccagc gccaccggca attatcccga tggcggcgcc acggttgaaa
    13621 tctattccaa ccacggcctc ggctatgcgg aaatcgaaac gctgagcccg gaagtcgcgc
    13681 tggcccccgc caccgtgctg gaaaatacgt tgtgcatccg gctggccgcc acgccagagg
    13741 aaaccggcgg ctgcaaactg gccgaggccg cgcagtcgct cgccgcgccc tgagcccttc
    13801 ccaaccaact gtctgctgac gagcaacccg cttcaacgcg ccggggtccg cagcgcaatc
    13861 tgcaaacaca tcaacgccca aaagcagccc aacagcgcgg ccagcggcat ttgcaacggc
    13921 gtgggcaacg catacagcac cagcaggcac ggcgcccaga acgtccagcc cggcacttga
    13981 ttcggcagca cgatgcgccg ataccacccc ggccccagtt gcaggcgcgt cgcgccccac
    14041 gaataattca aatcctgcca caggtgcgac accgccacca gcggcgacgc ataaaacggc
    14101 gaatacacca acatatccgt agcggttttc aacagcacgg tggcgaagtt ggcatcgtgc
    14161 ccccacaccc gcgcttgcag cgtataaaaa agatgcgtgt tcgcgcccat aaagccccac
    14221 cacaccacgc cgaagagcag cgccgcccac ggccgccgca cgcgcaaccc cggcaccagc
    14281 gcgcagaaaa ccgccggcac cacgccattg aacactgccc gcgtcaaaat gctgaacgtg
    14341 atgccgtagc gatgctgcca ctccgccacg tgctgcatcc aaccatgaaa ccgcggcgac
    14401 acataatagc ccgctaccag cgccaccgcc atcgcctgca agaccaaacc cggaatcaaa
    14461 ttcgcccgtg ccgcctgcca gccgatgcgc catggactgg cgcccacgac ttttccacac
    14521 tgtggaaact ttttttccat gctgtggaaa acctagtccc gccacgcgcg gacaaaatag
    14581 tcgtcgggca cgtcaatggc ttccgccacc cgccagccgg ccgcggccag ttgctgttgc
    14641 agcgccaccg ccgtgggcat atggtcgtgc tgcacggcgt gaagttgcgc gtgcatggca
    14701 ttcaattcgc ggctgccttc caaattggcc actaccagtt gcccccccgg cgtcagccag
    14761 tcccaacatt cgcgcaaaaa cgccgccgga tcaggcaggt gcggatagaa acgaaaaacc
    14821 agcacgatgt cccacgaccc cgcgggcgcg gaataaccgg taatatcgcc gtggcgcacc
    14881 gtcacttgtg gcaagccggc caccgccgcg gcacaacgcg caatcatgcg ttcgctggca
    14941 tccagcgcca gcacatgacc gccccccccc acccactccg ccaagcggac agtcaacggc
    15001 ccggcgccgc agcccggctc gaaaatccgc cgcccgcgca catcgccaag acgcgcgcgc
    15061 aaccgtgcca aacacggctc atgctccgcg cgaaacgcca gcgggtcatc ctccgcatgg
    15121 gccaaatcct caaaaaaatg actgcgttcc accatgatgg ggccgcacta tggcgcgcca
    15181 cccgccacgg aacaagccaa aaccgccgcg cgcaaaaatg gccaccgcgg gccgcttgcg
    15241 gcttgccccg gcacccgcaa atcgggacac tgaccgcatg aaattgggcc tgctctccga
    15301 cacgcataac cacctgctgg aaacccggcg cgcgctggat ttgctcctgc gccacggcgc
    15361 gcagcacctt gtgcattgcg gcgatgccgg cgaggatgtc gtggacttgc tttccgcgac
    15421 ctgccaagag cacggcctgc gcgcccatgt ggccatcggc aattgcgacc ggatgtatgg
    15481 cgccaactct gcactctatc cccagcccac cggcatcacg cgcggcgaat accttgagtt
    15541 tgtggtggcg ggaaaacgct gcctcgtgat gcacggcgat aaccgccgcc gcctcgcgca
    15601 agccatcgcc tccggcacct tggattatat tttcctcggt cacactcacg aaccgcatga
    15661 cgaacgatgc ggccccacgc gccttcttaa tcccggttcc gtcgcccgcc cccgccgtgg
    15721 cccacccacc gtcctcctcc tcgacctccc caccgatacc cccacctggc tccccctggc
    15781 ctagcccgtg gcatgtatat cagcgttcac ccttgtgcag cttgctgcgc ataaaagggc
    15841 aacggcttat tggccaaacg attgctagtc tgcttcggct ttcttttaaa tcccccgtcg
    15901 gaaggaagtg gggtggtgtc aaatgtcccg tttaatgcgc agggagggta gatttgatat
    15961 gaagatagtt cctgttttct taatctgggc ttcaatccaa taaatcgcgc ccgcgcaccc
    16021 tttaaataat cttcatgcaa ttcacaacat atccatttgc gttccagaga ttcagcaact
    16081 tcgcccgtta cgcatgagcc tgcgaatggg tccaatacca agtcgccggg gttggttaac
    16141 attcggataa aatactctgg aatacctgac gggaatcgcg ccggatgaac cggcagagaa
    16201 tttgccctgc aatattcctg atacccccca ttagattcag tatttgccaa cgcgattaag
    16261 ttaggcggaa ccgagccccc gttgtccaca ccaaacttgt cagaaatatc atgcccggat
    16321 ggccgcagtt taggggtata accattttta agaagcgatt tcatcgaatc gctataaggg
    16381 gctaataccc ggcgattaga tgccttaggc catggtgtac gcgatagcca ccaaatgcag
    16441 ttgattgcat ccttaactct aatacgtcga atatttaccc actcggccgg tgttggcagt
    16501 tttgccggat tccaccaata gtgttcttga caaagatgaa acccatactc cttgcataaa
    16561 gttaaaagaa gttcaaagtg gtaaagtgac tttgttggta gccctgcttt ccatgcgctt
    16621 ccgatatcta ttaccaaact accttccttt ttaagaactc ttttaaaccc tgcggcaaaa
    16681 gggcggaacc attggacata ttgatcagca tcctcattgc cataatcttt tttacgtact
    16741 aggccaaacg gcggagaagt cataaccaga tcaacagatt ctggcttcaa tgtgttgtgc
    16801 aaaagcccaa gtgagtcccc gagatacatt ttcccgtgct tggtttcctc aaaaagagtg
    16861 ggaagaagca gtggatatgt agcgctatct tctttcacga cttcttgctt ttgatgttgg
    16921 gcggccacca atgagtgaag attgcgtaga taaccgctaa gggagctaaa gccttcgcgt
    16981 tttgcgcaga cctccatctc atcatactga tcagaagtga gacgaaagtt tacaacccgg
    17041 cttcttggat ttgagctttt tggtcttggc atagtggtac atgtaagaca actacccgcc
    17101 aaggtcaaga tataatgtaa tacaactata tagttctaag aagcgcgata ttgattggcc
    17161 gctctgatat aggctagatt tggccactgc ctacgatctg cgggcaggaa tatctcccgg
    17221 ccaaggtgtg cctcgaactc aactaaacct ccgcccaagc caagtcgaac gagatcattt
    17281 ttcttcttag ggttaacaac catatggcaa tcttctgtca ggtagatcaa cccgcgatca
    17341 aacgcgcggt gataagttgg cgaaaggcag agcccatttg ttacctcatc tgtgctgcca
    17401 ctggccccta cagggagaat atgtgcggca tcaatcagac gcaattggag tccagtaacg
    17461 gcacattttc tatcgtaggc aatcgtgact ttacggcgga aatcactatc gcgaacaagc
    17521 cgcgacactt tggctaatat tctttttctt tcgggcggaa gcactgccgt ttcatcagcg
    17581 tcgatgggac ccagccgcgt gatcttttct aatgccgtaa gaacacgggc atctgtgccg
    17641 ctttcgtgca tactttctgc gttcagggca tatgccaata tgttatctgg tctaaaccca
    17701 atggctattt cgtcattacc tttccttgaa taagcaaagc catccctgat ggcatcgcgc
    17761 agagttttat agtttacctg gatggatggt gagccgacag aaaaggattt gtgtttacgc
    17821 agatcaaacc cggcaaaaca ctttatattg ggttcgtaac ccaaaagaag tgtcggccca
    17881 tctgggtttt ctggaagtgg cggagttacg ctggttagtt gaatacggta ttcgttacgt
    17941 ggtctggccg cccctccacc atgggtgatg gtccatatat atacccacaa tgggaatgct
    18001 gattcccctg ccacaacata aaaacgtcgc gggtgagacc ggacatctga gatgagcgta
    18061 gcagccgctc cggtatcagt aaaggcatca attattgcgg ctacaatttc ttttggcggt
    18121 aatgcggcca tgtactctcc ttgctttccg acaaaaatca acgatgaggc ggcagcagtc
    18181 tgtcgccacc ggtctcaagc gctaagttca actttttcat tttgcgcgaa tggggagttt
    18241 gagtccagaa aattggagtt ttggtaccat aaatggcgta ttttcttggt ttgggggcca
    18301 ttttggggtg tttttggccg ttttggggtc acaaaatcgc atttttacgt gttttcctgc
    18361 aacgtcaccg ctcccacgcg ggtattggaa ttttgtatgc cactcctgat ttgttaaaca
    18421 cctgctcccc ccccgttagc agcacggcgg tgtgaaaggc attgttctac acgttaaccc
    18481 atcttttcaa ccagccgccc attgatgaac ttgtgcagga tactggaaat cagcgtttga
    18541 tagggcaagc cctcttgcat ggcttttttc tggaggcgga caagatcgag ctcggtcatg
    18601 cggatattca cccgcttgtc cttgcgcaat gtggcctgcg ccgactcgcg ataacgggac
    18661 gattgatcca ggaaatccgg gattcgtttc cactccccct tttcagcgga gtctaatagt
    18721 tttttttcat cgttggtgag tttcatttta atcacctccc aaataatgtt tcgtcatttt
    18781 gcgactggga ataatcgttt tgagaaagat tttgtcgtca tctttcacga acggcacgat
    18841 gcacgcatag ccttcgacat tgacaacaaa aagccgttgc cccggatact gtcgtggatt
    18901 ggggcgttcc aagatgtcca gcaatccgtc gtgtgccagg tgaaatacaa tatcttcaaa
    18961 agtgatgcca cgctcctgtc tcagccattc attcttctcg tcgttccaat cgaatggttt
    19021 cattgtgcgg aacatatcac aatgtgtgcc ttatggcaac agcgcacaca agaaattggc
    19081 ggttggggac caaaaatggc gtattttacg ggtttttggg ttgttttggg gtgtttttgg
    19141 ccgttttggg gtaaaaaggg cggttttgtc cgctgtgcgt agcacgtgca aagcctcagt
    19201 ccagccacac gaagcccaac aggaaaccca acagtcccgc cctcaacgag agcggctaca
    19261 gaagcagcca atagccccgc tcgctctgct cttctgccag cggatcgttc ggcatcagcg
    19321 atcaggccgc cggcgtagat actccttgcc gccaacatca aacgcgaatg aatacgttag
    19381 ggcctgattc gggcaaacgg agatgcagtt ttgacaaaaa atgcattccg tcgaaagcac
    19441 ccgttgtcca tttttgatgt agcgggggat ttggatatcc atcgggcaat tccgcgtaca
    19501 caccccgcat tcgttgcatt tctcggcgtc gcccgtgatc ctgagcaggg aaaaccgagc
    19561 gccaattttc aatggcaccg taatggggca gacgtatttg caaaatgccc gattatcctt
    19621 gagtgcatag gccagcacaa tgccaatgag aaaataaaac ccattcccca ccagcaacca
    19681 ggcaagttcg gttgacccat gttgcggccg gtagttaaag ccaaaccaca aaaccaacac
    19741 gaggatcaaa ctgccagcga aatggatggc gcgcacccat tcccatggtc gggcaactcg
    19801 cccgggactt ttcgtatagg gcaaaaaatt cagaaccatc gttgtccagc aggcccagcc
    19861 gcaccacccc cgattgaata cgaggggccc gacgattttc gcaatcgcat aatggagggc
    19921 ggcggcggca aacatcccca tcaggacata gaaaaagaat ccttcgagtt gcatgttctc
    19981 ggacttggcc agcccaagaa acaccagcat atacagcccg accagcaact gcgttatttt
    20041 gcgaccaatg ggacgccgcc gcttgggcaa gaacgtgaac agtccgttgc cgatcccgat
    20101 ggcggtcccg atatacccaa aattaaataa ataaaagatg ctgcctttgg cctgccagag
    20161 aacaactgcg atgatccaaa aaatgatggg cagcgctgcc acttcaagga tgtttttcac
    20221 tttttgatcc atgatgtgcc tcctctttga tgcgtattac gtgcctggca acagcacgtc
    20281 aaggatgcgt tatcggcaga cgatctacgc agcgtcttgc tcaattccca gacttttcgt
    20341 cagatcgcgc ttgggccgcc gctgttcgcc gtgcgtggcg aatacaatca atatgtcttc
    20401 cacttccatc cggtaaaata ttttacaagg cggcagaaca gcctgccgga aagaatcttt
    20461 ccagccatgt ttggtgatat atcttggaca ttggctggca aaacaatgta cttgatttac
    20521 tcaatatctt gagtaatttt actcaacgat atgagcataa atcagaaatc atttatccga
    20581 cctcaggcgg ctgtattact tgaaaggctg cgggaacctc gccgttttat tcaggttgtc
    20641 agcgggccaa ggcaggtggg caagacaacc atggtgcagc aggtggtcgc acaattaacg
    20701 gtacctgctc gatatgccag tgccgacgag ccaacactgc gtgggccaac ttggattgag
    20761 caacaatggg atgcagcgcg actgtcggcc acttctgcgg aaggggcggt actgattctg
    20821 gatgaagtgc aaaaggtggg gaactggtcg gaaactgtca aggggctgtg ggatgaagat
    20881 acgcgcaagc ggctgccttt gcaggtggtt ttgctgggtt cgtcacctct gctcatatcc
    20941 aaggggctga cggaaagcct gaccggccgt tttgaaaaca gtattttgac gcattggtcg
    21001 tatgccgaga tgaaaaaagc attcggcact tcgctgaacg aatatgtttt ttatggcgga
    21061 tatcccggcg cggcatccct gatcggcaag ccggatcgct ggcgcaacta tttacgcaac
    21121 agtttgatcg aaaccacgat tgctcgcgat attctactca tgacccggat tgataaaccg
    21181 gcattgttgc gtcaattgtt tgaactcgcc tgccgctttt ccggacagat tctctcgttc
    21241 aacaagatgc tcggccaact tcatgatgcc ggaaacacga ccacgctggc gcactatctg
    21301 aatctgttgg ccgcggcggg catggtgtgt ggcttgccaa agtattctgg caacgtcatc
    21361 cgcagccgcg gctccagtcc aaaactgcaa gtactcaata cgggattaat gaacgctctg
    21421 ggttctgcct cgctaccgga gacacacaag caaccggcac gctggggacg gctaatcgaa
    21481 tccgctgtgg gcgcccacct tgccaacagc gcgctccggg gcgactgcgc tgtctattac
    21541 tggcgcgaag gcattgcgga agttgatttt gtggtgcagt gcaatgaccg cttaactgct
    21601 atcgaagtga aaagcggcca tgcaccaaaa atgcacaagg ggacggcggc cctcgccaaa
    21661 cggttcaagc tggaccgaac attgctgatc ggtggcgatg gtatcgcgtt tgaagaattt
    21721 ttaagcgaac ctgtcacgca ttgggtcgga taaatgatcg gctggggcca ctgcgaacca
    21781 ttttctctcg ccacgaagaa ttgtctgctt aacgaatatt ctgccctact gcacttgctt
    21841 ggcgcgcttg gcgagagaca cttcccttca tgctcat
//
LOCUS       DAOPNP010000033        21036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3116_length_21036_cov_9.056670, whole genome shotgun sequence.
ACCESSION   DAOPNP010000033 DAOPNP010000000
VERSION     DAOPNP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21036
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_21036_cov_9.056670"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..455)
                     /locus_tag="PKI64_04460"
     CDS             complement(60..455)
                     /locus_tag="PKI64_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOE00298.1"
                     /translation="MSEFSSTIQNADWKIEKHAPVIEAPDSVKADEFFTISAGLGKAV
                     AHPNTAQHYIAWISIYFLPDGGKFPIQLAHAEFKAHGDSTTDVPGPALTNPSMTASVK
                     LAKSGTLQALAYCNIHGVWQSAKAIRVEG"
     gene            635..1813
                     /locus_tag="PKI64_04465"
     CDS             635..1813
                     /locus_tag="PKI64_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00299.1"
                     /translation="MKTRWLVLILVFCCALPALAVEVTLKDAGDIRLQIYGYLKADYS
                     YDSQATAPKGDFAFFVLPELDGKKDGQTHLGARESRLGVNIFGPDVGAWKTTGKFEMD
                     FYGGEKPNSYNPRMRLAYVDVAHDSGFSLRFGQDWDTFCEIQPRIVNFAFLADIGALG
                     LRRPQARATQELKFSENTKLVLKAAVAQTVGEDLDGGGTEDGTDADYPTAQWNVTLHQ
                     KVWAGKTARLSFAGHYGTETVDAVDSNKVVIANDVQDFDTWSVQGSLYLPLCKTLAAQ
                     ANIWKGANLDTYFGGIGQGVNMALGREIKAVGGYAQLLYDPTDKLCFGVGYSVDDPDN
                     EDLNAGMRAKNSQVFANVFYKFTPAVTGMAEYAHMVTDYLGRDDATNDRVQVAVRYTF
                     "
     gene            complement(1916..2395)
                     /locus_tag="PKI64_04470"
     CDS             complement(1916..2395)
                     /locus_tag="PKI64_04470"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOE00300.1"
                     /translation="MNDHGITSLTGGESLDKSAPIICAVGSMDELNAALGLLRAILPA
                     GPDAEQVAEWQRQTLRIGAELATGRAQLPAEAVAALDRQLVEREAALPPPDGFVLPGN
                     NETTARAHWARVMCRRAERTVVAVRATHPERVFPATLAWLNRLSSCLFALARAYEPQ"
     gene            2562..3143
                     /locus_tag="PKI64_04475"
     CDS             2562..3143
                     /locus_tag="PKI64_04475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE00301.1"
                     /translation="MRKRTLHSCPTCRLVFADPRDWPAPAAEKERYATHRNGPEDAGY
                     VAFLQQAVTPALPLLPPGARGLDYGCGHTPTLHLLLQQAGFACANYDPFFFPDWPGET
                     FDYVFATEVVEHFHRPAREWPKLLSLLKPAGLLIVMTAPWDEQTDFRTWGYASDETHV
                     AFYHRETFAWIKARWGLAEIPNPNPRVLLLRRA"
     gene            complement(3165..4196)
                     /locus_tag="PKI64_04480"
     CDS             complement(3165..4196)
                     /locus_tag="PKI64_04480"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOE00302.1"
                     /translation="MSPTRPALTPQAAHRLLRGEIMPVVGCTEPAAIGYALRLLTQHL
                     PHPVQPGRWRVILRISREALRNASTAVVPHLRVRGVRAAAAAGIASSANDFNIFAAVD
                     LRRARAFLRASDWLEIVPVRRCGLYVQARLVGQRTSVTLAGRHDHIKQWMVAGRDRTP
                     VANQMPRPPTLADIFRLARAWNPRLENLARDFLLRQVPAEPGHKLETQIARRITGRMT
                     GFAHPVMTITGSGNQGIFIALPYRALLAKMGDAILPAVVFTLLAQVYLTAKHKRLSAE
                     CGIATKAAPALAAGLAFARGAGPAEVRRIFRDIPAQLAGLTCEGAEPACGRKARQAFR
                     AVAPWLAAL"
     gene            complement(4213..4848)
                     /locus_tag="PKI64_04485"
     CDS             complement(4213..4848)
                     /locus_tag="PKI64_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00303.1"
                     /translation="MKHRDLIFKELRAHAPFTALGSLAGIVLLLGLVWSQVPAGFSHQ
                     LFGILHPLHVLLSAQATIATYRRAGGAGFWRILLIGYVGGVGIGTLSDCLIPYLGEWL
                     LGLPNRGYHIGFIEEWWLVNPLALVGIALGVWSVRTRVPHGLHVLISTGASLFHIALA
                     MDGTPSLPVLGGIAVFLFLSVWIPCCTSDIVFPLLFAPNKDAITGCCHHHH"
     gene            complement(4877..5476)
                     /locus_tag="PKI64_04490"
     CDS             complement(4877..5476)
                     /locus_tag="PKI64_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE00304.1"
                     /translation="MLTQLQLCATHRWRVKRYRTLRFQPGLNLLIGPNGTGKSTVLRA
                     IANCPDCCRREDGPTEYLLFDAEQMNPRHSVGHIHTPTEMTLHLRAQFSSHGEILQAA
                     FTTLQAAPGTCLLLDEPESAQDFDHILALRAAMRRAAARGVQLIVATHEVLFWEPAHI
                     LELRRGYRERVTRTLCQMKCLHPEHSPARAPKRISGVDK"
     gene            complement(5476..5808)
                     /locus_tag="PKI64_04495"
     CDS             complement(5476..5808)
                     /locus_tag="PKI64_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOE00305.1"
                     /translation="MPRGDRTGPQGAGPMTGRGAGYCAGFATPGFARGGRGGWGAGRG
                     YGMGRGMGRGFGRGFGRGRGWPWAAPEMPAPATELATLQQQAKQLQQDLELIQSRIKE
                     LETPPDAT"
     gene            complement(5899..6219)
                     /locus_tag="PKI64_04500"
     CDS             complement(5899..6219)
                     /locus_tag="PKI64_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOE00306.1"
                     /translation="MKLGLVIYSTDAETVWNALRLGIFARQQGDEVRAFLLANGVECT
                     QHNTPPFAVTDQLQAFADAGGRILACGTCLQIRQQTGSELCPLSTMRDLYELVRDCDK
                     VLTF"
     gene            complement(6232..6570)
                     /locus_tag="PKI64_04505"
     CDS             complement(6232..6570)
                     /locus_tag="PKI64_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE00307.1"
                     /translation="MKIAIPVADGQLCLHFGHCAHFALVEVDDATKKITTNRLVEPPP
                     HEPGVLPRWLHEQGVEVILAGGMGSRAQQLFAQHGIRTVVGCAPAAPADLVAAFLAGD
                     LVTGQNACDH"
     gene            complement(6583..7425)
                     /locus_tag="PKI64_04510"
     CDS             complement(6583..7425)
                     /locus_tag="PKI64_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE00308.1"
                     /translation="MKAVRLTVLADNYVTARNLRGEHGLAFHLAADNRQILFDTGQGL
                     VLADNARALGIDLGAVDTVVLSHGHYDHTEGLPHVLGVARAPVTIHLHPQALQPKYRV
                     TGSEARSIGLPQEALDALSRPPARVMTATTPTEVAPGIFCTGEIPRPADGPPPTDIFH
                     LDPAGTQPDPLLDDQALYFSTTKGVVVLLGCAHSGVIPTLDYIRRLTGQPIRAVLGGL
                     HLGKAPPAEIQRVADYLRALELTCLVPMHCTGTRATAALWATLPNICREGGVGARFEF
                     EKEN"
     gene            complement(7422..8318)
                     /locus_tag="PKI64_04515"
     CDS             complement(7422..8318)
                     /locus_tag="PKI64_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE00309.1"
                     /translation="MKELVIISGKGGTGKTSLTASFAALYPSAVLADCDVDAADLHLI
                     LQPKIRRREKFRSGHAAVICAEACTGCGRCAEVCRFGAIAVAEPDETGRRLHSVAPTA
                     CEGCGVCVWNCPAQAIDFPEQECGEWFLSGTRHGPLVHARLVPGSENSGKLVSLVREQ
                     ARQLAQQEDREVVLVDGPPGVGCPVIAALTGAQGVLVVTEPTVAGEHDLHRVLQLADH
                     FRIPAAVCLNKWDLHPEQAARMEKAARAAGATPVGRIRYDDAFTAAQRQGRAVVETEA
                     SALGDDIRAVWTNWCAITGVTP"
     gene            complement(8315..9157)
                     /locus_tag="PKI64_04520"
     CDS             complement(8315..9157)
                     /locus_tag="PKI64_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE00310.1"
                     /translation="MPLTIAIASGKGGTGKTTVAVHLAAWLAEQGRPVHYLDCDVEEP
                     NGHLFLRPKWTVEEEVSIPVPAVDHDRCTRCGKCAAVCEFNAIAMLNKPLVFPELCHG
                     CGGCTLVCPVQAIREVPRVIGTMATGRAGKIQFTQGRLNVGEPLAPPVIRAVQARRAP
                     DAIALLDAPPGAACPAVATARGADVIILVTEPTPFGLHDLELAIATFRPLQKPLGVVV
                     NRADADRRVQEYCAGAGIPILAELPAERRVAELYARGELLFAALPPWRDMFAKLWQQI
                     KALQ"
     gene            complement(9187..9552)
                     /locus_tag="PKI64_04525"
     CDS             complement(9187..9552)
                     /locus_tag="PKI64_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE00311.1"
                     /translation="MKVVITASGPTLAEPIDLHFGRAKYFILADTETGRAKAHDNTPN
                     LQARQGAGVQSAETVANLGAEVVVTGSVGPKAYRALQAAGIKIALVEAVCPGTEALRR
                     FKDNELPVLDKPNSEGHWV"
     gene            complement(9549..9947)
                     /locus_tag="PKI64_04530"
     CDS             complement(9549..9947)
                     /locus_tag="PKI64_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOE00312.1"
                     /translation="MPRPYSRRFVGWQPTASLFKPAGIPHGALEHVDLSLEEVEALRL
                     ADLNGQYQEEAAGHMKISRATFARLCESARRKVADALVHGKALRIQTEPGAQPPPPAL
                     APGACRRRGCGCRGRGRGRYIEYPPRKEQP"
     gene            10159..11265
                     /locus_tag="PKI64_04535"
     CDS             10159..11265
                     /locus_tag="PKI64_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00313.1"
                     /translation="MSFESGSISFRMVEMPRVFPEDWAARFAAHRAGSLDRLTTGEQR
                     GWVTGRHLLDTQITEETAMLAGWVRLALRRAERKVPAALLKAECRLEELALMAAEGKA
                     YLKAQARAEIRQGVLDRLLPSMPPQLRALPFVYRPGAKHFYVSALPVPQLDVFMAAMQ
                     SALGFAGEPATPEVLGHRLRKTDLRDLPGSSFSPEMEREAMAVAPGREFLTWLWFKAE
                     TQEGRLALSAGQAVGVLVEGPLTFWHEGQGAYLSVLKKGMPETSMEAKACLLSGKKLK
                     EATITLALDEQNQWKFGLEADEFLIRNLKLPAGEGRLDAASRFLQRMEQLEQWREIFL
                     DLFDAFLAVRVEARKWQSTVADIREWVKARPARR"
     gene            complement(11345..11836)
                     /locus_tag="PKI64_04540"
     CDS             complement(11345..11836)
                     /locus_tag="PKI64_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOE00314.1"
                     /translation="MISKKMAKEINAQINREFFSAFLYLAMANDALDKGYNGTYRWMT
                     LQFQEEQEHALKFSKYLQDQGVKVELAAMEAPKTSWKGILEMYEDALAHEKKVTAWIY
                     GLNSLAIAEKDYATQSMLKWFIDEQVEEEASVSEAISAISMAGNSSGALYMVDHRLGK
                     RED"
     gene            12040..13539
                     /locus_tag="PKI64_04545"
     CDS             12040..13539
                     /locus_tag="PKI64_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOE00315.1"
                     /translation="MIHLIYVKNIISRTVGGTFQELSFAVRVRNVGFGKQVEVHWAGE
                     NGVWQVTPAEFRSELPDGEEVWVAETAIPLTRQSAIPGNIQFAARLTHDGISHWDSRQ
                     GQNYTLDADSGIVVLEPVDMRVVGCRPRLAPGMVALPIEVAVRSAVGPEAVTVHWTTD
                     GWATQHATQAHYRRDHWDRTVGSNARNPNRYGWEVWAARIPLQHAYRVEIAVECRARA
                     GQFWDNRSGRNYAARRGTLKVLTLNLHTYQEEDQLRKFARVARAIRERDIGIVCLQEV
                     GENWNDGAGDWASNAARIINSHLPEPYHLHTDWAHLGFEAYREGVAILSRYPFVYTDS
                     GYISDSRDPYDIHARKVVMAQVQAPYFGRLNVFSAHLSWPSGGFYPQFERLHQWAAEK
                     QTPDVMGTLLCGDFNIPAGSEAYRAIVDTGDYEDQFLKVMSGRVFHKIFRERAPNALD
                     LLARDGRIDYIWLRQGNRLRAIGAEELFTDHCYGRVSDHSGYLVEFEPR"
     gene            13556..15169
                     /locus_tag="PKI64_04550"
     CDS             13556..15169
                     /locus_tag="PKI64_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOE00316.1"
                     /translation="MEFAEQYAAPTDAYLSRYYPRRNEFKELFYLRWGRIRFRVEWGV
                     DMNVKVVLKVFREDGTMEQFLVHTDVCGAEWNRHWRETRDIFIHPQALHQGRITCVKF
                     AYLLHHQGRSIPSRLDYIFMDGPDVQQDGEVRRHVTEQWATPNPYRTYEVDAGQLQRD
                     VDWLNHHFDALGLIPKFTKGQAWHPYHPKRYIHDQIDTMIWRRQTQPGRACAIKVCVD
                     CIDDADFIRHLIHAHGNGVPVQCIVDWRKMTLTNSANYLALKRSGIELIGEVCTTRDP
                     LAEVATDMHNKFIIFGDEDAIVGSFNITFDRWGSNWESGMTFHSYGVTRLLDNIFQAC
                     RGGVIQRYGIDPLAPFNLLYTFGRTAMLNGKYYRPHQAILAEVSRARHSIQLCLFLIG
                     ELRGEHGDSVVDALIAAKHRGVYVRLILNGHLAWEGDPARERSMHEELYQRRLLPAVR
                     RLQEAGIAIALVYGVHDRAVPYSPVHSKQCVIDEQIVLDGSFNWYNTSTLSHDLLVVV
                     NHREIAQLYLEEARQILEGFRVHWISLAR"
     gene            15173..15799
                     /gene="tsaB"
                     /locus_tag="PKI64_04555"
     CDS             15173..15799
                     /gene="tsaB"
                     /locus_tag="PKI64_04555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOE00317.1"
                     /translation="MNKVLALEFSTAQASLALLAAGEVIATRAVAATHRRSQELFAAA
                     DELLAGASWHLREMDAFAVGCGPGAYTGLRVSLTAANGWALPTGRPVWTVASAAALAA
                     ETLAEDTSLAQVVIAGEARRGTIWAGLFARDPAYLVTQIGDWQLLPDAARGAGWPGVP
                     WVAAGRVPHAAWVGRLHATGRPSAAPEPIYLHPAVAIPPRFPAPPPTA"
     gene            complement(15855..16937)
                     /gene="serC"
                     /locus_tag="PKI64_04560"
     CDS             complement(15855..16937)
                     /gene="serC"
                     /locus_tag="PKI64_04560"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOE00318.1"
                     /translation="MSRAFNFNAGPAALPLEVLEIAQKELIDFNGTGMSIMEHSHRGK
                     AYDAVHAEAVANIGKLLGVTDDYQVLFLQGGASLQFAQVPLNLLGEGQTADYVNTGAW
                     ASKAIKEAQKVGKVNILADTSKEIPTNMPDFDALAYTPGAAYVHVTSNETIAGTQIQK
                     FPNTASPLVCDMSSDILSRAFDLKQFGLIYAGAQKNLGPSGVTLVVIRKDLLERAPTT
                     LPTMLQYRTQAKENSLYNTPPTFGIYILMLVSRWLLAKGGAAGIEKINRAKADKLYAA
                     FDNSGGYYRGSAVKHLRSIMNVTWRLPSEELEEKFIKEATALKMIGLKGHRSVGGCRA
                     SIYNAVGMDAIDALTSFMKDFQAKNG"
     gene            complement(17041..18339)
                     /locus_tag="PKI64_04565"
     CDS             complement(17041..18339)
                     /locus_tag="PKI64_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOE00319.1"
                     /translation="MSAAPSRTLVILAAGFGWNLVELHKPVTRLQLRPIAPGALALTC
                     PAQATLRTALPPSRHGVVASGWFHHDLARPFFWEQSAALVQGRRVWADARQAGETVGM
                     AFWQQSLGEEVDMVISPKPIHRHHGGIVMDCYSRPEPLYAELSAQLRRKFDLYHYWGP
                     LARAKVGRWIAAAARYLLSRAGAPDHLLVYLPTLDYDLQRYGPASPQAAAALAEVLAQ
                     IEPLVAAAEIQGREWLLTGDYAIEPVTARDGAIFPNRALRAAGFFHTRTTAKMAYPDF
                     FTARAFAVVDHQIAHIHVRNPADLPAVRERLATLDGVAEILDRGAQVERDLYFPARSG
                     DLLLVAAPGRWFAYPWWTAPREAPDYARQMDIHNKPGFDPCELFFGFPPPHTSQRTEK
                     IRGTHGRSGPGYEVATAGLFPEARSFLELAQGLQRRWAAR"
     gene            complement(18336..18698)
                     /locus_tag="PKI64_04570"
     CDS             complement(18336..18698)
                     /locus_tag="PKI64_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE00320.1"
                     /translation="MKSPRAQCLVAGFTLLEVLAALGLCALLATAAAGAVAFSLRAER
                     AAQRYGEATLLLPALYAAQQLHPDDAPPAPRGWRLQHNTDIVKIPDDLLQEWHRYTLQ
                     PDGREIPAITFFILDDNP"
     gene            18803..20509
                     /locus_tag="PKI64_04575"
     CDS             18803..20509
                     /locus_tag="PKI64_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOE00321.1"
                     /translation="MTARQLAAPAPEKGRGPRVATALTIAAVVVLALGLGGAGGLYRA
                     LQRQEDARVARGFQQAAETFFGRTLREVQLFMEVLDSIRQLHSLSDQVSPAAFEEIVR
                     KGMIYQRRILGAYGFVQRIPHSMRAGYETPGGAGHPIVQADDRGNFVPMASRPEYFPL
                     TYQTPPGGLGVQLGYDFGARPADAAAIAAAERLGVFALGGPVFGAAGSGEAYMFAPIM
                     YSDAPQSLVTVGYAIGLFQPAHILATARGDLPPGLHARLEENRVPAAPVGGLHFSKEF
                     SLANQEWRFVVTADSTYATAVRSRAPELSAALLAAAALLFAALLLRMAGRTRRIEALV
                     SQRTVELAAANRALQAAMEDRRKLEDEVLRISGREKARIGSDLHDSLGQKLTGAMYLF
                     GAYRQRVGAPDDAAAADAAQIAATLKDAVTQVRRIARGLAPVALTENGLPDALRHLAG
                     ESQALFQKNVEFFAEREGQPQNAGTAEHLYLIAQEAVNNAARHSGATRIVITLDYDER
                     GGELTIEDNGRGLSAAAPADDLGGSGLRIMRHRAEVFGGELFIEPVPAGGLRVRCRFP
                     HA"
BASE COUNT         3820 a         6579 c         6744 g         3893 t
ORIGIN      
        1 agtgtggaaa aaagttttcc acactgtgga aaagtgtgcc gcggcgggtg gggcgcaggt
       61 taaccctcga cccggatggc cttggccgac tgccaaacgc catggatgtt gcaataggcc
      121 aacgcttgca gcgtgccgga cttggccagt ttgacgctgg cggtcatgct ggggttcgtc
      181 agcgccgggc cggggacatc cgtggtggaa tcaccgtggg ccttgaactc ggcgtgcgca
      241 agctgaatcg ggaacttgcc gccgtcgggc aggaaataaa tcgaaatcca cgcaatgtag
      301 tgctgggcgg tgttcgggtg ggccacggcc ttgccgaggc cggcgctaat cgtgaaaaac
      361 tcgtcggcct tcacgctgtc cggcgcttca atcacagggg cgtgtttctc gattttccaa
      421 tcggcgttct gaatggtgct gctgaattcg ctcatggttt tctcctttgg tttgatttgt
      481 tgaaaaacag gccagcttaa cacgacccgc cccccgccgt cactggcgaa gtggcgaaat
      541 atttgcattt tcccgagaaa caaggttgac cttgctccta ttctccccaa aaataaccgg
      601 agaatataaa gaaacctcaa ggagacccct gcccatgaag acacgttggc ttgtcctgat
      661 tttggtgttc tgttgcgcgc tcccggctct cgcggtcgaa gtgaccctga aagacgcggg
      721 cgacattcgc ctgcaaatct acggctatct caaagccgat tattcttatg atagccaggc
      781 caccgctcca aaaggcgatt ttgccttctt tgtactcccc gaactggatg ggaaaaaaga
      841 cggccagacc cacctgggcg cgcgtgaaag ccgccttggg gtaaatatct ttgggccgga
      901 tgtcggcgcc tggaaaacca ctggcaagtt cgaaatggat ttctacggcg gcgaaaagcc
      961 caattcctac aacccgcgga tgcgtcttgc ttatgtggat gtggcccatg attctggctt
     1021 ctcgctgcgc ttcggccagg attgggatac cttctgcgag attcagccgc gcatcgtcaa
     1081 ctttgccttt ttggcggata tcggcgcgct cggcctgcgc aggccccaag cccgcgcaac
     1141 gcaggagctg aagttctcgg agaacaccaa gctggtctta aaggccgcag tggcgcaaac
     1201 cgtcggtgag gacctcgacg gtggcggcac cgaggatggc accgatgccg attatccgac
     1261 ggcccagtgg aatgtcaccc tccaccagaa agtgtgggcc ggcaaaaccg cgcgcttgtc
     1321 cttcgccggc cactacggca ccgaaaccgt ggacgcggtg gatagcaaca aggtggtgat
     1381 cgccaatgac gtgcaggatt ttgatacctg gtccgtgcag ggctcgctct acttgccgct
     1441 ttgcaagacg ctcgccgcgc aggcgaacat ctggaagggc gccaatctgg atacctattt
     1501 cggcggcatc ggccaaggtg tgaacatggc cctgggccgc gaaatcaagg ccgtcggcgg
     1561 ttacgcgcaa ttgctgtatg acccgaccga caaactgtgc ttcggcgttg gctattccgt
     1621 ggatgatccg gataacgaag acttgaacgc aggaatgcgc gcgaagaact cacaggtctt
     1681 cgccaacgtg ttctacaagt tcacccccgc cgtcaccggc atggcggaat atgcccacat
     1741 ggtcaccgat taccttggcc gcgacgatgc caccaacgat cgcgtgcaag tggccgtgcg
     1801 ctatacattc taaacaatac ggtaggttgc gaccccaagg ccgtccggcc caccggacgg
     1861 cctttcttat gtgcggtcag cgccgtggcg gccgccgaca gaaaagagcc gtattctatt
     1921 gtggctcgta ggcgcgggcc aaggcaaaga ggcagctcga aagtcggtta agccaagcga
     1981 gggtggcggg gaatacgcgc tccggatggg tggcgcgcac ggcaaccacc gtgcgttcgg
     2041 cgcgtcggca catcacgcgt gcccagtggg cccgcgccgt ggtttcgttg ttgcccggca
     2101 gcacaaatcc gtccggcgga ggcaaggcgg cttcacgttc aaccagttgc cggtctagcg
     2161 cggccaccgc ttctgcaggc aactgtgcgc gcccggtcgc caactcggcg ccaatgcgca
     2221 gcgtctggcg ctgccactcg gccacctgtt ccgcatcggg ccccgccggc aaaatggccc
     2281 gcagcaaacc cagcgccgcg ttgagttcat ccatgctgcc caccgcgcaa atgatgggtg
     2341 cggatttgtc cagcgactcg ccgccggtca gggacgtaat tccgtggtca ttcattcctt
     2401 ccagcctagt ccgaaatgcc acgcggcgca actgcgcgcc gcagtagcgg cgggagttct
     2461 cacctgctac agtgggcgac atggtttcaa cccaacaaac cgcgccttcg ctgacttgcc
     2521 cactgtgttc cgccgccggg ccatttgcgc cggtgcgcga tatgcgcaaa cggaccttgc
     2581 attcctgccc aacgtgtcgg ctcgttttcg ccgatccgcg cgattggccc gcccccgccg
     2641 ccgaaaaaga acgctacgcg acccaccgca acggcccgga ggatgccggc tatgttgcct
     2701 tcctgcagca ggccgttacg ccggctctgc ccttgttgcc ccccggcgcg cgggggctgg
     2761 actacggctg cggccatacg cccactctcc atctgctttt gcagcaggcg ggcttcgcct
     2821 gcgccaatta cgacccgttc ttttttcccg attggcccgg cgagacgttc gattatgttt
     2881 tcgccacgga agtggtcgag cattttcacc gtccggcgcg cgagtggccc aagctgctga
     2941 gcctgttgaa acccgctggc ctgctcattg taatgacggc cccgtgggac gagcaaaccg
     3001 acttccgcac gtggggctac gccagcgatg aaacccacgt ggctttttac caccgcgaga
     3061 ctttcgcctg gatcaaagca cggtgggggc tggcggaaat cccgaatccg aatccgcgcg
     3121 tgctgctgtt acgccgggcc tgatgcccgc gcgcatggcg cggtttacag cgccgccagc
     3181 cacggcgcaa ctgcgcgaaa ggcttgccgg gccttgcggc cgcaggccgg ctccgcgcct
     3241 tcgcacgtca gtcccgcaag ctgcgcgggg atgtcgcgga agatgcgccg gacttccgcc
     3301 ggcccggcac cccgtgcaaa agccagcccc gccgcaagcg ccggcgctgc tttggtggcg
     3361 ataccgcatt ccgccgacag ccgcttgtgc ttggctgtca aatacacctg cgccaacagc
     3421 gtgaacacca cggccggcag aatggcgtcc cccattttcg ccaacagcgc gcgatagggc
     3481 agggcaatga aaattccttg gttgcccgag ccggtaatgg tcatcaccgg atgggcaaag
     3541 ccggtcatgc ggccagtgat gcgccgcgca atctgggttt ccagcttgtg cccgggctca
     3601 gccggcactt gccgcaagag aaaatcgcgc gccagatttt ccagccgggg attccacgcg
     3661 cgggccaagc ggaaaatatc ggccaatgtc ggcggacgtg gcatttggtt ggcgacgggg
     3721 gtacggtcgc gtccggccac catccattgt ttgatgtggt cgtgtcgccc ggccaacgtg
     3781 acgctggtcc gctggccaac cagtcgcgct tgcacataaa gcccgcagcg gcgcaccggc
     3841 acaatctcca gccagtcgga tgcgcgcaga aaagcccgcg cacggcgcaa atccaccgcg
     3901 gcaaaaatgt tgaaatcatt ggcgcttgaa gcgatgcccg ccgctgccgc ggcgcgcacc
     3961 ccccgcactc gcaagtgcgg cacaaccgcc gttgaggcgt tgcgcagggc ctcccgcgag
     4021 attcgcaaga tcacgcgcca gcgccccggc tgtacgggat gaggcaggtg ttgcgtcagc
     4081 aagcgcaacg cgtagccgat ggccgcgggc tcggtgcagc ccaccacggg catgatttcg
     4141 ccgcgtagca gccggtgcgc cgcttgggga gtcaatgctg ggcgggtagg ggacatttgg
     4201 cgcagagtgg ggctagtggt gatggtggca acagccggtg atggcatcct tgttgggggc
     4261 gaacaacaac gggaaaacaa tgtcgctggt gcagcagggt atccacaccg acaaaaacag
     4321 gaagacggca atcccgccca acaccggcag ggagggagtg ccgtccatgg ccaatgcgat
     4381 gtggaagagt gaggccccgg tgctgatcaa tacgtgcagc ccgtgcggca cgcgggtgcg
     4441 tacgctccac acccccagcg cgatgcccac cagcgccagc ggattcacca gccaccattc
     4501 ttcaatgaaa ccgatgtgat aaccccgatt gggcaggccc agcagccatt cgcccagata
     4561 cgggatgagg cagtcgctca atgtgccgat gcccaccccg cccacgtagc cgatcagcaa
     4621 aatgcgccag aatcccgcgc cacctgcccg ccggtaggtg gcaatggtag cttgcgcgct
     4681 cagcaatacg tgcaaggggt gcagaatgcc gaaaagctga tgggaaaacc cggcgggtac
     4741 ctggctccac accaagccca gcagcaagac gatgccggcc aacgagccca acgcggtgaa
     4801 cggggcgtgt gcgcgcagtt ccttgaaaat caaatcacgg tgcttcatgg gttgttgcag
     4861 tgaatagaaa aaccgttcat ttgtccacgc cggaaatacg cttgggcgcc cgcgccggcg
     4921 agtgttcagg atggaggcac ttcatttggc aaagggtgcg cgtgacccgc tcccggtaac
     4981 cgcggcgcag ttccagaatg tgcgccggct cccagaacag gacttcgtgc gtggccacaa
     5041 tcaattgtac gccccgcgcc gcggcgcgcc gcatggccgc gcgcagggcg aggatatggt
     5101 cgaaatcctg tgcggattcg ggttcatcca gcagcaggca ggtgcccggc gcggcctgca
     5161 gcgtggtgaa cgcagcttgc aggatttcgc cgtgcgacga aaactgcgcc cgcaggtgca
     5221 gcgtcatctc cgtcggcgtg tggatgtggc cgacgctgtg ccgagggttc atttgctccg
     5281 cgtcgaacag caggtattcc gtcggcccat cctcacgtcg gcagcagtcc gggcaattgg
     5341 cgatggcgcg cagcaccgtg cttttgccgg tgccgttggg gccaattagc aaattcaagc
     5401 ccggttgaaa acgcagtgtc cggtaacgct tcacccgcca gcggtgggtg gcgcacaact
     5461 gcaactgggt cagcatcagg tggcgtcagg cggggtttcc aattccttga tccgggactg
     5521 gatcagttcc aagtcttgtt gcagttgctt ggcttgctgc tggagcgtgg ccagctcggt
     5581 agctggggcg ggcatttccg gtgcggccca aggccatccc cgtccgcgac caaagccccg
     5641 gccaaaaccg cggcccattc cccggcccat cccgtatccc cggccggcac cccagccgcc
     5701 gcggccgcca cgggcaaagc ccggtgtggc aaatcccgcg caatatcccg cgccgcgccc
     5761 ggtcatgggc cccgcgcctt gtggtcctgt tcgatctcct cttggcatgt catgtctcct
     5821 cttgttgcgc ggccgttacc agccgcctat tccacataat gaacatatgc tcattacgcg
     5881 actttgtcaa gcagtcgcct aaaacgtcag caccttgtcg caatctcgca ccaattcgta
     5941 cagatcgcgc atggtggaga gcgggcacag ttcggagcct gtttgctgcc ggatttgcag
     6001 gcacgtgccg cacgccagaa tccggccccc ggcatcggcg aaggcctgca actggtcggt
     6061 taccgcaaaa ggcggggtat tgtgctgagt acactccacc ccgttggcca atagaaacgc
     6121 ccgcacctcg tcgccctgtt gtcgggcgaa gatccccagc cgcagcgcat tccagacggt
     6181 ctccgcatcg gtggaataaa taaccaggcc cagtttcatg ttggcctcct gctaatgatc
     6241 acaggcattt tggccggtga cgagatcgcc ggccaaaaag gcggccacca agtccgccgg
     6301 cgcggccggg gcgcatccca ccaccgtgcg gatgccatgt tgcgcgaaca gttgttgcgc
     6361 gcgcgagccc atgccaccgg ccagaatcac ctccaccccc tgttcgtgca gccagcgggg
     6421 gaggacaccg ggttcatgcg gcgggggctc gaccaaccgg ttggtggtga ttttcttggt
     6481 ggcgtcatcc acctcgacca aagcaaaatg agcgcagtgg ccaaagtgca agcacagttg
     6541 gccgtcggca acaggtatgg cgattttcat aacggagtcc tttcagtttt ctttttcaaa
     6601 ttcaaaacgt gcgcccacac cgccctcacg gcaaatattc ggcaaggtcg cccataaagc
     6661 cgccgtggcc ctggttccag tgcagtgcat tggcacaagg caggtcaatt ccaacgcgcg
     6721 gagatagtcc gcaacacgtt gaatttcggc gggtggcgct ttgcccaaat gcaggccacc
     6781 cagcaccgcg cggatgggct ggccggtgag ccggcgaata tagtccaacg tggggatcac
     6841 tcccgaatgc gcgcacccca gcaagaccac tacgcctttc gtggtggaga aatataacgc
     6901 ttggtcatcc agcaacgggt caggctgggt gccggcaggg tccagatgaa agatatctgt
     6961 tggcgggggc ccgtcggctg ggcgcggaat ttcaccggtg caaaaaatac cgggcgctac
     7021 ttcggtgggg gtggtggccg tcatcacccg ggcgggtggc cgggagagtg cgtcaagcgc
     7081 ttcttggggc aggccgatgg aacgggcttc gctgcccgtg acgcgatact tgggctgcaa
     7141 cgcctgtgga tgtagatgga tggtcaccgg cgcgcgcgcg acgcccagca catggggcag
     7201 gccctccgtg tgatcgtagt ggccgtggct caacaccacc gtgtccaccg cacccaaatc
     7261 aatacccaag gcgcgggcgt tatccgccag caccaatcct tgaccggtat caaacaagat
     7321 ctgccgattg tcggcggcca aatgaaacgc cagcccgtgt tcgccgcgca agttacgggc
     7381 cgtgacataa ttgtcagcca gcacggttaa gcggacggcc ttcatggggt cactccggtt
     7441 atggcgcacc aattggtcca caccgcgcgg atgtcgtcac ccagtgcgct ggcctcggtc
     7501 tcaaccacgg cacggccttg gcgttgggct gcggtgaagg catcgtcgta gcgaatgcgt
     7561 cctaccggtg ttgcgccggc ggctcgcgcg gctttttcca tgcgcgccgc ttgctccggg
     7621 tgcaaatccc atttgttgag gcaaaccgcg gccggaattc ggaagtgatc ggccaactgt
     7681 aaaacgcggt gcaggtcatg ttccccggcc acggtgggtt ccgtcaccac caacaccccc
     7741 tgcgcgccgg tcagggccgc aatgaccggg cagccgacgc ccggcgggcc atccaccaag
     7801 accacttcgc ggtcttcctg ctgcgccagt tgccgcgctt gttcacggac caacgagacc
     7861 aacttgccgg aattttccga gcccggcacg aggcgggcat gaaccaacgg cccgtggcgc
     7921 gtgccggata aaaaccattc gccgcattct tgctcgggaa aatcaatcgc ctgcgcgggg
     7981 caattccaaa cgcaaacccc gcacccctcg caggcggtgg gggccacgga atgcagtcgg
     8041 cggccggttt catcaggttc cgcgacagcg atagccccaa agcgacagac ttccgcgcaa
     8101 cgcccacagc cggtgcatgc ttccgcgcag attaccgctg cgtggccgct gcggaacttt
     8161 tcgcggcggc gaattttcgg ttgcaagatc agatgcaggt cggcggcgtc cacgtcgcaa
     8221 tcggccaaca ccgccgacgg atagagagcc gcaaacgacg cagtcaagct ggtcttgccg
     8281 gttccgccct tgccgctgat gatcaccagc tctttcattg gagggccttt atctgttgcc
     8341 agagcttggc gaacatgtct cgccagggcg gcagcgcggc gaagagcaat tcaccacgcg
     8401 catagagttc ggccacacga cgctcggcgg gcagttcggc cagaatgggg atgccggcgc
     8461 cggcgcaata ctcctgcaca cggcgatccg cgtcagcccg gttcaccacc acccccagcg
     8521 gtttctgtaa cgggcgaaac gtggcaatag ccaactccag atcgtgcaaa ccaaagggcg
     8581 tgggctcggt caccaggatg atgacatccg cgccccgcgc ggtggcaacc gccggacacg
     8641 cagcgcccgg cggcgcatcc agcaaagcaa tggcgtccgg cgcgcgccgg gcttgaaccg
     8701 cacgaatgac cggcggcgcc aacggctcac cgacattcaa gcgaccttgg gtgaactgga
     8761 ttttgcccgc gcgccccgtg gccatcgtgc cgatgacccg gggcacttcg cggatcgctt
     8821 gcaccgggca aaccagagtg cagccgccgc acccgtggca gagttcaggg aaaaccaagg
     8881 gcttgttgag catggcgatg gcgttgaact cgcacacggc cgcgcacttg ccgcaacggg
     8941 tgcaacgatc gtggtccacc gctggcacgg ggatgctgac ctcttcttcg accgtccatt
     9001 tgggccgcag gaagagatgg ccgttgggtt cttccacatc gcaatccaag taatgcaccg
     9061 gccggccctg ttctgccaac cacgccgcca gatggacggc tacggtggtt tttcccgtgc
     9121 ctcccttgcc actggcaatg gcgatggtga gtggcatgac agttgcgact cccgcacaac
     9181 gcggctttag acccaatggc cttcggagtt cggcttatcg agcacgggca gttcgttatc
     9241 cttgaaacgc cgcagagctt ctgtgcccgg gcagaccgct tccaccaagg caatcttgat
     9301 gccggccgcc tgcaatgcgc ggtaggcttt ggggccgaca ctgccggtca cgaccacttc
     9361 cgcgccgagg ttggccacgg tttccgccga ctgcacgccc gcgccctggc gagcttggag
     9421 attcggggtg ttgtcgtggg ccttcgcgcg gccggtttcg gtatcggcca aaataaaata
     9481 tttggcgcgg ccgaaatgca gatcaatggg ctccgccaag gtgggcccgc tggcggtaat
     9541 gacgactttc atggttgttc ctttcgtggg gggtattcga tatagcgacc gcgcccgcga
     9601 ccgcggcaac cgcacccgcg gcggcggcac gcgccgggag cgagggcggg agggggcggt
     9661 tgggcgccgg gttccgtctg gatacgcagc gctttgccgt gcaccaaggc gtcggccact
     9721 ttgcggcggg cggactcgca tagccgggca aatgtagccc gcgaaatttt catgtggccg
     9781 gcggcttctt cctgatactg gccattgagg tcggcgaggc gcagcgcctc gacctcttcc
     9841 aggctcaggt ccacgtgttc caaagccccg tgcggaatgc cggcgggctt gaagagcgag
     9901 gcagtcggct gccaacctac aaaacgtcgg gaatagggtc gcggcatggg tgggtttcct
     9961 ccgatatgtt caatctggaa atgaacatat gcccataatc agcaaaaggc aaccccaaaa
    10021 atcagccggg tgcgcacgcg gtgaaaaaat aaaaacatga aatcatcgga ctgccccctt
    10081 ttctcacctt tgggggtcaa cgggcttgcg gggcgggggc ggggatggca gagtcggcgg
    10141 ctgcgtaagg agagtgttat gtcgttcgag agtggttcaa ttagttttcg gatggtggag
    10201 atgccgcggg tattcccgga ggattgggcg gcgcggtttg cggcgcatcg ggcgggatcg
    10261 ctcgatcggc tgaccactgg ggagcaacgc ggctgggtga ccggtcgcca tttgctggat
    10321 acgcagatta cggaagagac cgccatgctg gcggggtggg tacgcctggc gctgcgccgg
    10381 gcggaacgca aagtacccgc cgccctgctc aaagcggagt gccgcttgga ggagttggcc
    10441 ctgatggcgg cggagggcaa ggcctacctc aaggcgcagg cgcgcgcgga aatccgccaa
    10501 ggggtgctcg accgtttgct gccatccatg ccgccgcaac tgcgggcgct gccatttgtg
    10561 taccggccgg gcgccaagca tttttatgtg tcggccctgc cggtgccgca actggatgtg
    10621 ttcatggcgg cgatgcagtc agcgttgggc ttcgccggtg aaccggcgac gcccgaagtg
    10681 ctggggcacc gattgcggaa aacggatttg cgcgacctgc ccgggtcgag cttttcgccc
    10741 gagatggagc gcgaggccat ggcggtggcg ccgggccgcg agttcctgac gtggctctgg
    10801 ttcaaggcgg aaacgcaaga gggtcggttg gcgttgtcgg ccgggcaggc ggtcggggtg
    10861 ttggtggaag ggccgctgac attctggcac gaggggcagg gggcctattt gagcgtgctt
    10921 aagaagggca tgcccgaaac cagcatggag gccaaggcgt gcctgctgag tggcaaaaag
    10981 ttgaaggaag cgaccatcac gttggcgctg gatgagcaga atcaatggaa attcggcttg
    11041 gaggcggatg agtttttgat ccgcaatcta aaactgccgg cgggcgaggg gcggctggac
    11101 gccgccagcc gcttcttgca gcgcatggaa cagttggagc agtggcgcga gattttcctg
    11161 gatttgttcg atgcgttttt ggcggtgcgg gtggaagcgc gtaagtggca gagtacggtg
    11221 gcggacatcc gggagtgggt caaggcgcgg ccggcccgac gttaatcgcg agcggggggg
    11281 cgcggcaaag aaaacgcggg gggcaagggc ctcccgcggt gggacaaacg gtttgtccgt
    11341 tggtttaatc ttcgcgtttg ccgaggcggt ggtccaccat gtaaagcgcg ccgctggagt
    11401 tgccggccat gctgatggcg ctgatggctt cgctgacgct ggcttcttct tcaacctgct
    11461 cgtcaatgaa ccacttaagc atgctctgcg tggcgtaatc cttctcggcg atggccagcg
    11521 agttgaggcc atagatccag gcggtgacct tcttttcgtg ggcgagggcg tcctcgtaca
    11581 tttccagaat gcctttccaa gaggtcttgg gggcctccat ggcggcgagt tccaccttca
    11641 cgccctgatc ctgaagatac ttggaaaact tgagtgcgtg ttcctgctcc tcctggaact
    11701 ggagggtcat ccagcggtaa gtgccgttgt agcctttgtc gagggcgtca ttggccatcg
    11761 ccaaatagag gaatgcggaa aagaattcgc ggttgatctg cgcgttgatt tccttggcca
    11821 tttttttgct gatcatgtga gctcctttgg gttgtccgtg ctattgtggc cgcgcccccg
    11881 gggcacggcg tttcattggg ggtagcctaa cacagggggg cgggtttgtc acatgaatcg
    11941 cgccggaagc gaagccgggc cgaataaagc cttgcggggg ttggcgggcc tcccctagag
    12001 tgcgggtgtc cgccggcgga tgcccgcggc ggattgacca tgattcacct tatttatgta
    12061 aaaaacatca tttcacggac ggtgggcggc acctttcagg agctgtcgtt tgccgtgcgg
    12121 gtgcggaatg tggggtttgg caagcaggtg gaggtgcatt gggccggcga aaacggggtg
    12181 tggcaggtca ccccggccga gttccgcagc gaactgccgg atggggagga ggtctgggtg
    12241 gcggaaacgg ccattccgct gacgcggcaa tcggccattc cgggcaatat ccaatttgcg
    12301 gcgcggctga cgcacgatgg aatttcgcat tgggacagcc ggcaggggca gaactacacg
    12361 ctggacgcgg attcaggcat cgtggtgctg gagccggtgg atatgcgcgt ggtggggtgc
    12421 cgcccgcggc tggcgccagg catggtggcg ctgcccatcg aagtggcggt ccgcagcgcc
    12481 gtcggaccgg aggccgtgac ggtgcattgg accacggacg gctgggccac acagcatgcc
    12541 acgcaagccc attaccgccg cgaccattgg gaccgcacgg tgggcagcaa tgcgcgcaat
    12601 cccaaccgct acggctggga ggtgtgggcc gcgcgaattc cgctccagca cgcctatcgc
    12661 gtggaaatcg ctgtggaatg ccgcgcacgc gccgggcagt tctgggacaa ccgcagcggg
    12721 cgcaactatg ccgcacgccg cggtacgctg aaggtgttga cgctgaatct ccacacctat
    12781 caggaagagg atcaattgcg gaaatttgcg cgggtggccc gggcgattcg cgagcgcgat
    12841 attggcatcg tgtgcctgca ggaagtgggc gaaaactgga acgatggcgc cggcgattgg
    12901 gccagcaacg cggcgcgcat catcaacagc cacctgcccg agccctatca tttgcacacc
    12961 gactgggcgc atctggggtt tgaggcctac cgcgaaggcg tggccatcct gagccgctat
    13021 ccgtttgttt ataccgattc cggctacatt tcggatagcc gcgacccgta cgacatccac
    13081 gctcgcaagg tggtcatggc gcaagtgcag gcgccgtact tcgggcgact caacgtgttc
    13141 agcgcgcacc tgagctggcc cagcggcggt ttttatccgc agtttgagcg cctgcaccaa
    13201 tgggccgcgg aaaagcaaac gcccgatgtc atgggcaccc tgctgtgcgg cgacttcaac
    13261 attcccgccg gcagcgaggc ctaccgggcc atcgtggaca ccggcgacta cgaagaccag
    13321 ttcctgaagg tgatgtccgg ccgcgtattc cacaaaattt tccgtgagcg cgcacccaac
    13381 gcgctggact tgttggcgcg tgacggacgc atagattaca tttggctgcg ccaaggaaac
    13441 cggctgcggg ccatcggcgc ggaggaattg ttcaccgacc attgctacgg ccgggtttcc
    13501 gatcacagcg gctatctggt ggagttcgag ccccgctagc cgggaagtgg accgtatgga
    13561 atttgccgaa caatatgccg cgccaaccga cgcttatttg agccgctatt atccgcggcg
    13621 caacgagttc aaggagctgt tctacctgcg ctgggggcgg attcgcttcc gcgtggagtg
    13681 gggcgtggac atgaacgtga aggtggtcct caaggtgttc cgcgaggacg gcaccatgga
    13741 gcaatttctg gtgcacacgg atgtgtgcgg cgcggaatgg aaccgccatt ggcgcgaaac
    13801 gcgcgacatc ttcatccatc cgcaggcgtt gcaccagggg cggatcacgt gcgtcaagtt
    13861 cgcctatctg ctgcatcatc agggccgctc gatcccctcg cggctggatt acattttcat
    13921 ggacggtccc gacgtgcagc aggatggcga ggtgcgccgg cacgtcaccg agcaatgggc
    13981 cacgccgaat ccgtaccgca cctacgaagt ggatgccggc cagctccagc gcgatgtgga
    14041 ctggctcaac caccacttcg atgcgctggg cctgattccc aagttcacca aggggcaggc
    14101 gtggcacccc taccacccca agcgctacat ccacgaccag attgacacca tgatttggcg
    14161 gcgccagacc cagccgggcc gggcctgcgc catcaaggta tgcgtggact gcattgacga
    14221 tgccgacttt atccgccacc tgattcatgc ccacggcaat ggtgtgcccg tgcagtgcat
    14281 cgtggattgg cgcaagatga ccctgaccaa cagcgccaac tatctggccc tcaagcgttc
    14341 cggcatcgag ctgatcggcg aagtctgtac cacccgcgac ccgctggccg aagtcgccac
    14401 cgacatgcac aacaagttca tcatcttcgg cgatgaagat gccattgtcg gctcgttcaa
    14461 catcaccttc gaccgctggg gcagtaattg ggaatcgggc atgacgttcc actcctacgg
    14521 cgtgacgcgg ctgctggaca atatttttca agcatgccgc ggcggcgtga tccagcgcta
    14581 cggcattgac ccgctggcgc cgttcaattt gctctatacc tttggccgca cggccatgct
    14641 caatggcaaa tattaccgcc cgcaccaggc cattttggcg gaggtgtcgc gcgcgcggca
    14701 ctctatccag ctctgcctgt tcttgattgg cgagctgcgt ggcgaacatg gcgacagcgt
    14761 ggtggatgcg ctcattgccg ccaagcaccg cggggtgtac gtgcggctga ttcttaacgg
    14821 ccacctcgcg tgggagggcg accccgcccg cgaacgctcc atgcatgagg agctctacca
    14881 gcgtcgcctg ctgcccgccg tgcgccgtct gcaagaggcc ggcatcgcca tcgccctcgt
    14941 ctatggtgtg catgaccgcg ctgtgccgta ttcgccggtg cattccaagc agtgcgtgat
    15001 tgacgaacaa atcgtgctcg atggcagctt caactggtac aacacttcca cgctctccca
    15061 cgatttgctc gtggtggtga accaccgcga aatcgcccag ctttatctgg aagaggctcg
    15121 ccaaatcctc gagggcttcc gcgtgcattg gatcagcctg gcccgttgac gcatgaacaa
    15181 ggtgctggca ttggaatttt cgactgcgca ggccagcctg gcgctgctcg cggcgggcga
    15241 ggtgatcgcc acgcgcgcgg tggccgccac ccaccggcgc agccaggaat tattcgcggc
    15301 cgcggatgag ctgttggccg gagccagctg gcacttacgg gagatggatg cctttgccgt
    15361 gggttgcggg ccgggggctt ataccggctt gcgcgtcagc ctgacggccg ccaacggctg
    15421 ggcgctgccc accggccgtc cggtctggac cgtcgccagc gcggcggcgc tcgccgcgga
    15481 aacgttggcg gaagatacgt cgctggcgca ggtggttatt gccggtgaag cgcggcgcgg
    15541 tacgatttgg gccggactgt tcgcgcgcga tcccgcctat ttggtgacgc aaatcggcga
    15601 ctggcaattg ctgccggatg ccgcgcgcgg tgccgggtgg ccgggtgtgc cgtgggtggc
    15661 tgccgggcgc gtgccgcacg cggcctgggt ggggcggctg cacgccaccg ggcggcccag
    15721 cgccgcgccc gagcccatct atctgcatcc ggcggtggcc atcccgccgc gtttccccgc
    15781 gccgcctccc acggcataaa aaaacgcacc gcgaaaatcc gcggcgcggt tggcattttt
    15841 tgccgttgcg tggcttagcc gttcttggct tggaagtcct tcatgaatga agtcagcgca
    15901 tcaatggcat ccatgcccac ggcgttgtag atgctggcgc ggcagccgcc gacggagcga
    15961 tggcccttca ggccgatcat cttcagcgcg gtggcttcct tgatgaactt ctcttccagc
    16021 tcttcgctgg ggaggcgcca agtgacgttc atgatggagc gcagatgctt caccgcgctg
    16081 ccgcggtagt agccgccgga gttatcaaac gcggcgtaaa gcttgtccgc cttggcgcgg
    16141 ttgattttct caatgccggc ggcgccgccc ttggccagca gccagcgcga caccagcatc
    16201 aggatataga tgccgaaggt gggcggcgtg ttgtagagcg agttttcttt cgcctgtgtg
    16261 cggtattgca gcatcgtcgg cagcgtggtt ggagcgcgtt ccagcaagtc cttgcggatc
    16321 accaccaggg ttacgcccga agggccgagg ttcttctgcg cgccggcata aatcaggccg
    16381 aattgcttga ggtcgaacgc gcgcgagaga atgtcgctgg acatgtcgca caccagcggc
    16441 gaggcggtgt tggggaattt ctggatctgc gtgccggcga tggtttcgtt ggaagtcacg
    16501 tgcacgtagg cggcgcccgg cgtataggcc agcgcgtcga agtccggcat gttcgtcgga
    16561 atttccttcg aagtgtctgc cagaatgttg accttgccga ccttctgggc ctccttgatg
    16621 gccttgctgg cccacgcgcc ggtgttcaca tagtcggcgg tttggccttc gcccagcagg
    16681 ttcaagggaa cctgcgcgaa ctgcaagctg gcgccgccct gcaggaacag cacctgatag
    16741 tcgtcggtca cgcccagcag tttgcctatg ttggccacgg cttcggcatg gacggcatca
    16801 taggccttgc cgcggtggct atgctccatg atggacatgc ccgtgccgtt gaaatcaatc
    16861 agctcctttt gggcgatttc gaggacttcc agcgggaggg ccgccggacc ggcgttgaag
    16921 ttgaatgctc tgctcatttg gttttccttc tctggttgtt tgacatcaca ataagcgcga
    16981 atcatacgca atccggccgg caaaaatcaa atctgaaccc acccgcgcgc gcagcacgtt
    17041 ttaccgggcg gcccagcggc gctgcaggcc ctgcgccaac tccaagaacg agcgagcttc
    17101 agggaacagg ccggcggtgg ccacttcgta accggggccg gaacggccgt gcgtgccgcg
    17161 gattttttcg gtgcgctggg aggtgtgcgg gggcggaaaa ccgaagaata gctcgcaggg
    17221 gtcgaagccg ggtttgttgt ggatgtccat ttgccgtgca tagtcggggg cctctcgtgg
    17281 cgcggtccac cacggatagg caaaccagcg gcccggtgcc gccaccagca gcagatcgcc
    17341 ggaacgcgcg gggaaataaa gatcgcgctc gacttgcgcg ccgcggtcaa gaatctccgc
    17401 cacgccgtcc agcgttgcca accgctcacg caccgccggc aggtcagcgg gattgcgcac
    17461 atggatgtga gcaatctggt ggtccaccac cgcgaacgcg cgcgcggtga agaaatcggg
    17521 atacgccatt ttggccgtgg tgcgcgtgtg gaagaacccc gccgcgcgca gcgcccggtt
    17581 ggggaagatg gcgccatcac gcgcggttac gggctcaatg gcgtaatcgc cggtgagcaa
    17641 ccactcgcgc ccttgtattt cggcagcggc taccagcggc tcgatttgcg ccaacacctc
    17701 cgccaacgcg gcggccgcct gcggcgaggc ggggccatag cgctgcaagt cgtagtcgag
    17761 cgtcggcaaa tacaccagca ggtggtccgg cgcacccgcg cgtgacagca gataccgcgc
    17821 ggcggcggca atccagcggc ccaccttcgc gcgggcgagg gggccccagt aatgatacaa
    17881 gtcaaacttg cggcgcagtt gcgcgctcaa ctccgcatag agcggctccg gccgcgagta
    17941 gcaatccatg acaatccccc cgtgatgccg gtgaatcggc ttgggcgaaa tcaccatgtc
    18001 cacttcttcg ccgaggctct gctgccagaa cgccatgccg acggtttcgc cggcctgccg
    18061 ggcatcggcc catacgcgcc ggccctgcac cagcgcggcg gattgctccc agaaaaacgg
    18121 ccgcgccaaa tcgtggtgga accagccgct ggccaccacg ccgtggcgcg acggcggcag
    18181 cgccgtgcgc agcgtggcct gcgcggggca tgtcagcgcc agcgcccccg gcgcgatggg
    18241 ccgcaactgg agccgcgtga ccggcttgtg caactccacc agattccagc cgaagccggc
    18301 ggccaaaatc accaatgtcc gggagggggc ggcgctcatg ggttgtcatc caaaataaaa
    18361 aacgtaatgg ccggaatctc gcggccgtcg ggctgcagcg tgtagcggtg ccactcttgc
    18421 agcaggtcgt ccgggatttt cacaatgtcg gtgttgtgtt gcaaccgcca cccccgcggc
    18481 gcgggcggtg catcgtcggg gtgcagttgc tgcgcggcat acagtgcggg caaaaggagc
    18541 gtggcctcgc catagcgttg ggccgcgcgt tcggcgcgca aagaaaaagc caccgcgccg
    18601 gcggccgccg tggccagcag cgcgcacagt cccagcgcgg ccagcacttc cagcagcgta
    18661 aagccggcaa caagacattg ggcacggggc gatttcattg gcacacccca atgtggcacg
    18721 ggcgcgccgg aggggcaacc ttgttctcgc cgcgtttttc gtgtcgcccc gccgcgggcg
    18781 cgggtgtaat cttgcccgcg tgatgactgc ccgccaactc gccgcgcccg cacccgaaaa
    18841 gggccgcggt ccgcgcgtgg ccaccgcgct cacgattgcc gcggtggtgg tgctggcgct
    18901 ggggctgggg ggcgcgggcg ggctatatcg cgcgctgcaa cggcaggagg atgcgcgtgt
    18961 ggcgcgcggc ttccagcagg ccgccgagac gttcttcggc cgcactttgc gcgaagtgca
    19021 actgttcatg gaagtgttgg attccatccg tcaactgcac agcctgtcgg accaggtttc
    19081 gcctgccgcg tttgaggaaa tcgtacgcaa gggcatgatc taccagcggc gcattctggg
    19141 cgcctatgga tttgtgcaac gcattccgca ctccatgcgc gccggctatg aaacccccgg
    19201 cggcgcgggc catcccattg tgcaggccga tgaccgcggc aacttcgtcc ccatggcttc
    19261 gcgtccggaa tattttccgt tgacctacca aaccccgccg ggcgggctcg gggtgcaact
    19321 gggctatgac ttcggcgcgc gcccggctga tgccgccgcc atcgccgccg ctgagcgcct
    19381 cggcgtgttc gcgctgggtg ggccggtgtt cggcgcggcc ggcagcggcg aggcctatat
    19441 gttcgcgccc atcatgtata gcgacgctcc gcaatcgttg gtgacggtcg gttacgccat
    19501 cggcctcttc cagcccgcgc atattctggc caccgcgcgc ggggatctgc cccccggcct
    19561 ccacgcgcgg ctcgaagaaa atcgcgtccc ggccgccccc gtcggcggcc tgcacttcag
    19621 caaggaattc agcctcgcca atcaggaatg gcgatttgtg gtgacagcgg attccaccta
    19681 tgccactgcc gtgcgctccc gcgcgccgga actcagcgcc gccctgctgg ccgcggcggc
    19741 tctgttgttc gccgcgctcc tgctgcgcat ggccggccgt acgcggcgca ttgaagcgct
    19801 ggtcagccag cgcaccgttg aactcgctgc cgccaaccgc gcgctgcaag ccgccatgga
    19861 agaccgccgc aaattggaag acgaagtcct gcgcatttcc ggccgcgaaa aagcgcgcat
    19921 cggcagtgat ctgcacgatt cgctcggcca aaaactaacc ggcgccatgt accttttcgg
    19981 tgcctaccgc cagcgcgtcg gcgcaccgga cgatgccgcc gccgccgatg ccgcccaaat
    20041 cgccgccacc ctcaaagatg ccgtcaccca ggtgcgccgc atcgcccgcg gcctcgcgcc
    20101 cgtagccctg acggaaaacg gcttgcccga cgccttgcgc catctggccg gcgaatcaca
    20161 ggcgctcttc cagaaaaacg tggagttttt tgccgaacgc gaaggccagc cgcaaaacgc
    20221 cggcaccgcc gagcacttgt acttaatcgc gcaggaagcc gtgaacaatg ccgcccgcca
    20281 tagcggcgcc acgcgcattg tcatcacgct cgactacgac gaacgcggcg gtgaattgac
    20341 catcgaagac aacggccgcg gactctccgc cgccgcgccg gccgatgatc tcggcggcag
    20401 cggactacgc atcatgcggc atcgtgccga agtgttcggc ggcgaattat tcatcgaacc
    20461 cgttcccgcc ggcggcctcc gcgtccgttg ccgcttccca catgcctagc cgcctcgccg
    20521 gacgctcggc ggcagaggtc agaggtcaga gttcaggaaa cagccacgcc tttgtaggcc
    20581 ggggttgcat ccccggcggg ctgttcggct gttctggctg ttcccctgaa acctgcaacc
    20641 tttaacctgt aaccatcttc cccctgtagc cggcctcgct gaggccgggg ctgttggctg
    20701 ctggttttct cctgacctct gacctcagac ctctgacctc tcctcccgcg gtcaccgccc
    20761 gcggctacag aaacagcgtc cttgtaggcc ggggttgcat ccccggcggg ctgttcggct
    20821 gctcaaccaa gcaccaaaaa ccaagcacca cgaaccaaga accctgtagc cggcctcgct
    20881 gaggccgggg ctgttggctg ctggttttct cctgacctct gacctcagac ctctgacctc
    20941 tcctcccgcg ttcaccgccc gcggctacag aaacagcgtc cttgtagatg ctatggggtt
    21001 tcgttcatgc atcatggtga tgtggatcaa ccacaa
//
LOCUS       DAOPNP010000034        21020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3120_length_21020_cov_8.959981, whole genome shotgun sequence.
ACCESSION   DAOPNP010000034 DAOPNP010000000
VERSION     DAOPNP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21020
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_21020_cov_8.959981"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="malG"
                     /locus_tag="PKI64_04580"
     CDS             complement(<1..533)
                     /gene="malG"
                     /locus_tag="PKI64_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014593714.1"
                     /note="with MalKFE is involved in the transport of maltose
                     into the cell; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HOE00322.1"
                     /translation="MNKGQRWRLFWAHAALLGFIALMLVPLLMVVSISLRAGNFATGG
                     ILPSAETFSWEHWRLVLGLPVLQPDGTWAAATVPVLRWFWNSIKVAAVSAFLILMLSA
                     TCAYAFARMRFRGRDGLLGGLLILQMFPMVLALIAIYSILEGLGRWVPALGLDTHPGL
                     ILAYLGGIATHIWMIKG"
     gene            complement(530..2074)
                     /gene="malF"
                     /locus_tag="PKI64_04585"
     CDS             complement(530..2074)
                     /gene="malF"
                     /locus_tag="PKI64_04585"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HOE00323.1"
                     /translation="MMFRGLARVLWLTVVAGIGWLAVQMALAGAAWLGMVFVAAGLGA
                     AWIYNSRRTHAARYLLPGLATFAIFVLMPLVYTVYIAFTNFSGEHLLRQEQVRAWFAQ
                     DWHTPDPARYNFRLHPAAEPGSYQIVVPMGNGEIGRNLLISRPFTPAEAAAGQPVTLA
                     LNILPPTAPAVGIREVIAARPWLNHARFSAPGKAVPLRLVSLRALGYRVPLWREDGEA
                     LVNVASGQTLRPDAARGNYVDATTGEAVGPGWRVWIGPENFRQIFRDPAIRAPFLQIF
                     GWNIAFALLSVVITFLLGVVLASLLQWPALRGRHIYRTLLILPYAIPAFIPILVFKGL
                     FNEGYGEINHVLQALWGIAPKWFTNPWLARLMILIVNTWLGYPYMMIIASGMLQAIPD
                     EMYEATAMDGAGPWTNFSRITLPQIFPPLFPLLIASFAFNFNNFTLIYLLTGGKPDIV
                     GSATVAGTTDLLVSYTFRMAFKDSSARFGFASAVATLLFVVVALLAWLQLRASRPRAA
                     AGGGRA"
     gene            complement(2071..3264)
                     /gene="malE"
                     /locus_tag="PKI64_04590"
     CDS             complement(2071..3264)
                     /gene="malE"
                     /locus_tag="PKI64_04590"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HOE00324.1"
                     /translation="MTWLRPILWCLALSALPLSAPAWEPQQLLVWINGDKGYEGIAEI
                     GRIFEQNTGIPVVVEHPEGATDKFFHAAKSGKGPDIMIWAHDRVGEWADSGLLLPVDP
                     DPAFQARIFPKAWEAFTHRGRLWGYPLAMESPGLIYNRALIGEDEIPTNLADCAKLAP
                     RLQARGAAPIMWDYNNAYFSFGLLAADGGYIFGQRPDGSYDLADIGVNHPAAVAAMTA
                     VTDLIRAKVLPGSLSYSIAEAQMNQGKLAMFISGPFAWENLKKSGIDFGVTTIPGVNG
                     HPARPFVGVVGAVFNRTSPNLDLAQEFLEHYLITPQGLAAMDRHVPLGVPALMAAYEA
                     QAHDPRLAGSMRNIEVGLLMPNIPQMGVFWSSMESALATITAGRATPQEALDNAAQRM
                     RRGAP"
     gene            complement(3345..4571)
                     /locus_tag="PKI64_04595"
     CDS             complement(3345..4571)
                     /locus_tag="PKI64_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HOE00325.1"
                     /translation="MAYQEKTTTTYGSRLSNSLKGIVFGLIAFVVGTVLLFWNEGNFV
                     KTKRALTEAEGAVVSVASVDTRAPELEGKLIHASAKANTADVLSDSAFGVSENAIALA
                     REVEYYQWVEESKTETRDKMGGGQEKITTYTYKKKWCSDPEDSLEFHDPAYQGKNWTW
                     LRFDEQTQYADTVTFGAYVLPDFFVESITGAESAQVQPTAEQTAAWTATIAKNPHAPA
                     ADNADDEETVPPAQWVHTDGSTVYFGLEPATPAIGDVRVTFSKVPPKEVSLIGRVQGD
                     TFVKYVAKNGRTVSRLEVGMVSAEEMFAHAQSENRIFTWLFRLLGVVLVCGGLRGIFG
                     ILEALAKVIPPLGTIVGAGITLVSLVLGGAWSLVWIAIAWLTYRPLVGIPLLVVAVGG
                     LIWLKGKSRKAAAPTA"
     gene            4835..6910
                     /gene="rpoD"
                     /locus_tag="PKI64_04600"
     CDS             4835..6910
                     /gene="rpoD"
                     /locus_tag="PKI64_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOE00326.1"
                     /translation="MSSKKKKTSTSAKPKAKKSAKPAVKPAGKPVAKPAKKPAAKVAK
                     PVKTAAKPVKTAKPAPKPAKSAKLAAKTAKAAKPAAKPVSKTQPTKKSGAKPAVVAKG
                     AKIPAPAQASKPVKGAPRGKAAQGAPPVAAPTPAAVLSGKNAPDYMLQIISYARERGN
                     KISSDELHEILPPEIIGVEEIERIEEKLRSLNIEISRDGGDLEGAIQADKATPATRAD
                     SSVIDDPVRMYLKQMGQVPLLTREQEVEISKRIEEAEQHAKRLLHRFGFATKAYFDMV
                     DRLQTSRERFDRVIADRPEGDKGAKPETEKREIGRDKYFGLIIPKLTKSVPQTQAVVR
                     ERFRALSASRLTIAEQERRKKLFVQARERLANMLNEFYFKQKAVEDFVVYADELARSF
                     ETLCKQIRDAEKSRSPAKKQHLRAHKKALRELEEEQCLCVEEFLQQHAELKDWLRKGL
                     RAKSEMVEANLRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYQRGYKFSTYA
                     TWWIRQAITRSIADQARTIRIPVHMIETINKLMRISKQLVQTYGREPTPEEIAEEMNL
                     PVDRVRAVMKMSQQPISLQSPVGDSDDTHYGDFIEDKTAENPSEMTAYSLLKERLSDV
                     LGTLTPREQEVLTLRFGLGDGYSRTLEEVGKKFKVTRERIRQIEAKALRKMRHPTRIR
                     KLEGFLEND"
     gene            6969..8306
                     /locus_tag="PKI64_04605"
     CDS             6969..8306
                     /locus_tag="PKI64_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOE00327.1"
                     /translation="MSTTIKQIVKRDGNVVAYDRDRIATAIFKAATAVGGSDRAEAEK
                     LAMQVEQQLEANFSGGTPSVEEIQDIVEEVLIKNGHAKTARAYILYRHNRAMERTQRA
                     YKFEATDNIPYKKIYEVLRWNMDHGCETVDGLNDIIARGKLPELIAACEERYDKEVRA
                     GAEKLIERLPEVRVMFIAGPSSSSKTTTTIKVSEKLAEAGHEFLALNVDHYFFNLEQH
                     PKDEFGDYDYETPQALDLKLIDEHVAALLAGKSIKTPHYDFKTGQRTLNVYDMQLAPN
                     QILLIDSLHGLYRDMTQSTPDTKKFKLYIETLGQVRNREGMFMRWADNRLLRRMIRDS
                     WHRNLQPEQTLTHWHYVRRSELRNIIPFIGTVDYLVNSAMPFELPILKARLFKYFPPA
                     IEKFKNEPKRQDAYVRAKRVYETLKDLTPVEDDSPVPKTSLLREFIGGSTYKY"
     gene            8338..8661
                     /locus_tag="PKI64_04610"
     CDS             8338..8661
                     /locus_tag="PKI64_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00328.1"
                     /translation="MFSTLWKINFHSVELLPPICRFARYGSLARPAAPVAIELAFAHR
                     PAGSGSALAYFPQCGKTFAKLFHCVEKSRKLFPLRGKNPPPKPEFFHTVENFFPHYGK
                     LAHAG"
     gene            complement(8743..10716)
                     /locus_tag="PKI64_04615"
     CDS             complement(8743..10716)
                     /locus_tag="PKI64_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HOE00329.1"
                     /translation="MNRYYYSDRIEQFLQTDTTHLIGILAENSEFEILVTQRNAWREQ
                     IAILKATLQGFSGQILFEYSIPRMGARIDVVLLIGACLFVLEFKVGETEYSAHAMDQV
                     WDYALDLKNFHETSHNAPIIPILVATAAKSQPLQLKSGVHNDKVFVPVKCNAETLHDL
                     LTLTLREVQDTPLETATWAQGRYWPTPTIIEAAIALYRGHQVAEISRSDAGAINLRET
                     TQAISGIIANAKAKGHKAICFVTGVPGAGKTLVGLNIATKHLDAADQLYSVFLSGNDP
                     LVKVLQEALARDQICRAKAQGQMLTKGVAHSRVRAFVQNVHHYRDDCVSNPLPPADHV
                     ALFDEAQRAWNKQQTCKFMRQKKGHGDFDQSEPEFLISCVDRHPDWGVVICLVGGGQE
                     INTGEAGIAEWIDALNRRFRNWHIHISKRLTDKEYDAQRVLKEIAQRDHVTYHDDLHL
                     SVSMRSYRAEHVSQLVKEILDVHPAEARATLQKISANYPIVLTRNIHRAKEWLRQQAR
                     GSERYGLVASSSAERLRPQCIHVKAPMNPIHWFLAGKDDIRSSYYLEDVATEFDIQGL
                     ELDWICVTWDGDLRLTGEQWRHFSFVGKRWNRVKQPERQKYLENAYRVLLTRARQGMV
                     IVVPEGDPSDPTRAAEYYDPTFKYLQGIGFPTI"
     gene            complement(10823..13501)
                     /gene="acnA"
                     /locus_tag="PKI64_04620"
     CDS             complement(10823..13501)
                     /gene="acnA"
                     /locus_tag="PKI64_04620"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOE00330.1"
                     /translation="MSQPIATLKTFTHNGRTVAYHSLAALTELRGSSLAELPYIVRIL
                     LESAVRNQGHPAYNWRQVEALARWRPGQTAAQEEIPYLPARVLLQDLTGVPCVVDLAS
                     LRSAVVRNGGDPGIIEPLVPVDLVVDHSVQIDCWAMPDALNQNIEIEFSRNRERYEFL
                     KWGQQAFKKLRIVPPGVGICHQVNLEFLADGIHVAEQADGTWLAYPDSLVGTDSHTPM
                     VNGLGVLGWGVGGIEAEAAMLGQPIALLTPVVTGVRLSGALRPGVTATDLALTITQLL
                     RQHNVVGQFVEYFGPGCASLSLSDRAVLSNMAPEYGATTGFFPMNAETIAYLRMTGRP
                     AAHCELVEEYARRQGLFQEPDSPEPAYSRVIELDLGTVVSAIAGPKRPHDFQPIGEIK
                     RRFLAALGAAPEKGGFGVPAERQQAAALLAGGDELRHGSVAIASITSCTNTSNPVLML
                     AAGLLAKKAVARGLRVPPHVKTSLIPGSRVVTAYLKSSGLLPEMEKLGFGVAAYGCGT
                     CIGNSGPLRDDIAQAVRDQDLVVAAVLSGNRNFEGRVHPQCKANYLCSPPLVMAFGLA
                     GRVDINLDEEPLGTGADGQPVFLRDIWPTAAEIDALLATANDPALYAATYQDVNQYTP
                     EWNSIAVDRKPVFDWQPDSTYIREPPFLDAPPPNPDLSGARVLGYFGDFITTDHISPA
                     GSIAKDSPAARYLLERGIAPKDFNSYGSRRGNHEVMMRGTFANVRLRNRLVCREGGWT
                     RLLPAGDEMSIFDAAMAYQQTGTPLIVLAGQQYGAGSSRDWAAKGPLLLGVRAVIAES
                     FERIHRSNLVEMGILPLEFPTGQTAEDLGLDGTETYAIRAEGPLAPRGWHEVTAVKTD
                     GSEIRFRAQNRIDAPIEVAYYTAGGILPYVLRHLTT"
     gene            complement(13614..13689)
                     /locus_tag="PKI64_04625"
     tRNA            complement(13614..13689)
                     /locus_tag="PKI64_04625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13654..13656),aa:Ala,seq:ggc)
     gene            13859..14176
                     /gene="rplU"
                     /locus_tag="PKI64_04630"
     CDS             13859..14176
                     /gene="rplU"
                     /locus_tag="PKI64_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOE00331.1"
                     /translation="MEAYAVIETGGKQYRVQKDDVLSVELLADAKVGQQIELGRVLAF
                     NDGSGLKIGAPEVAGVKVAAEVVEEYRAAKVVAFKKKRRKGYHRKVGHRQSLNKVKIA
                     SIA"
     gene            14192..14446
                     /gene="rpmA"
                     /locus_tag="PKI64_04635"
     CDS             14192..14446
                     /gene="rpmA"
                     /locus_tag="PKI64_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOE00332.1"
                     /translation="MAHKKGQGSSRNGRDSHGQRRGIKRFGGQTVTAGSVLVRQCGTK
                     KTPGLNVGMGKDYTLFALVDGVVSFDRGGKRVNVQKLAQA"
     gene            14515..15534
                     /gene="obgE"
                     /locus_tag="PKI64_04640"
     CDS             14515..15534
                     /gene="obgE"
                     /locus_tag="PKI64_04640"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOE00333.1"
                     /translation="MKTLTFVDRVRIQVFAGNGGNGVALFRREKFIPHGGPDGGDGGN
                     GGSVFLRASKDEASLLDLHFAPLVRAEHGEHGRGKQQYGRNGQDKIVPVPLGTAVFDA
                     ETEELVGEVLQDGDLLKVAQGGKGGWGNIHFKTSTHQAPTEFTEGTPGEIRTLRLVMK
                     TVAEVGLVGYPNAGKSTLLTQLSAARPKVAAYPFTTLHPQVGILKFDATHAIRVADVP
                     GLIKGAHQGVGLGHDFLRHIERTAFLLFVIDMAGVDGRDPTADFRSLREELKLYNPEL
                     AARPYLVLANKMDEPAAQKNLAAFKRKTKEKPLPMSAELGEGVPELKARLFAQFFPGE
                     TILDI"
     gene            15588..16571
                     /locus_tag="PKI64_04645"
     CDS             15588..16571
                     /locus_tag="PKI64_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00334.1"
                     /translation="METNPPPSDSFNEFGYRKKRPARAADDAPRGPRPFRKPYRAGRD
                     DAPPVAEGEGQDFRRPYRPPYRDQDRPPRPYPRRDERGAHAPGGFRKPYRPRHDDGQP
                     PRDGAGQDFRRPRREDDRPRGPGGYRKPYRPRTTEPGDQESSAGYRPRRPGGGHFRKP
                     YGGPRSDGPGGSSGPRGGYGRPYGRPAAGEGQERGFRKPYSPRPGGYKKPYGPPRGEG
                     AGGYRKPYGPPRSEGEGGYKKPYGPPRGSRPGGGFRKPYGPPRGEGEGGYRKPYGPSR
                     GPRPGGGFRKPYERGPGGPRKPYSGPRKPYGDGQRPTWRKNEGADDAPPQA"
     gene            16679..17386
                     /locus_tag="PKI64_04650"
     CDS             16679..17386
                     /locus_tag="PKI64_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00335.1"
                     /translation="MAKKEEQQTANGVSAKSALIFEMDNIALGARRIRYEVLAGILKE
                     EKIGLSPVQFSRYCLHPAPSVYMESFLETMKYKSATPEQVVDRLTNETVSHLMQKSTT
                     INPGLIKWIEAAQARGAAVACISMLPQAAAEGVAAHLHFERWGIKVFSATPTDKGFPH
                     AKNWLQMAKAIARHPRRCLAIVSSMTVAKTALAAMMNIVVVPDEFTEYQDFCGANLVC
                     ADLKDVNPDEYFEQTKF"
     gene            17481..18251
                     /gene="mazG"
                     /locus_tag="PKI64_04655"
     CDS             17481..18251
                     /gene="mazG"
                     /locus_tag="PKI64_04655"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOE00336.1"
                     /translation="MARLLKIMARLRRPGDGCPWDLEQTIESIKPNLIEEAYEALDAM
                     ESGNRVDLAEELGDLLLQIIFQAQIAAEEGTFNFADVAQGISDKLVRRHPHIFGDVKV
                     RDSKDVLRNWDAIKKTEKKVRKSALDGVPKHVPPLHRAYQLQRRAARVNFDWPDIADV
                     FKKLDEEVRELKTAVNRKQRQGIIEELGDLLFSVVNVARFMKVDPAYALERTNAKFVR
                     RFEVVEKEITASGRKLKDCTLAEMDAIWNRVRAADKRA"
     gene            18208..19395
                     /locus_tag="PKI64_04660"
     CDS             18208..19395
                     /locus_tag="PKI64_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00337.1"
                     /translation="MPSGTAYARPTSARKTGWLALGLAGLLAMAAPAAEPPLARLAIW
                     CEFMPYAEVARELPTLAEYDCDLILHVERESFADPDFAPLVRAAAQAGVRVDAWLLLP
                     YDEHLYVGQASLAATRDLARQAVEYFTQEKLAVRAFVFDCEPSPLLGRELFAAVRAKS
                     PRQLAGVLRAQLNPTQFLRDVAELNELIAGLHASGYAVDGAANRAFLDAQARGNVALE
                     DALNAPVTGVTWDALSFITYRYRVGQESYVAMLNRYATLARRLYGVRAAVDVGLIGDY
                     TAIPQNAERAALFGGGADFYAYLRGMRDTRDLEQAVGVVLGCGVRRVNLYALDGAATS
                     VAGTESWLRAARQARPRGAWARWTPLGSLQYGALGALTEKMFRWTTGGTGKAYEKKEK
                     TES"
     gene            19367..19999
                     /locus_tag="PKI64_04665"
     CDS             19367..19999
                     /locus_tag="PKI64_04665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOE00338.1"
                     /translation="MKKKKKQNRKLILASASPRRREYFRQLGYPFRVATPLTQERPRR
                     GEDAAEYVLRNAQEKAMDVAQRVSPEAVIVAADTVVVLRGKIMEKPQSARGAQAMLRA
                     LSGQVHQVITGFCVYEPGRKGPLKSWAQAVVTDVEFKKLSAAEIKAYIESGEPMDKAG
                     AYAIQGRAAYMIRQIQGSYTNVVGLPMTELVEVLTGEFGLHPTFAGPARA"
     gene            complement(20009..>21020)
                     /locus_tag="PKI64_04670"
     CDS             complement(20009..>21020)
                     /locus_tag="PKI64_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00339.1"
                     /translation="KPSFLCDIMSLIYARLNNLLRQVWPPCYILEDKSVTRKARIPYN
                     YVELFAGEASLVAEDNRFALLKLNPPTEPAAEAHRMVRHDYLVQYPCITFSARTPAGS
                     PPVTLWLDLNGYVIGRWTIGPEARDIHVDIPARYAVKLTPNLFRFRTEDAMPFFLDSF
                     AAARHPGPLTEPALDCATANCPLPLGHIHQIPAEAIPLKIRFTSGFRLIGCEPLQTTA
                     PAGGAFRLRYYLQCPRDLEIITGSVLATRVTAASGKWVEERSIIAAVCDLNDLQCQIH
                     PGIYTLEQTIQLPEHFAPGDYVLSVLLRTEKGQRIYGMSNKKPGRIFVLPVPIRILAA
                     DD"
BASE COUNT         4385 a         6359 c         6528 g         3748 t
ORIGIN      
        1 tagcccttga tcatccagat gtgcgtggcg atgccgccga gataggcgag gatcaggccg
       61 gggtgggtgt cgagcccgag cgccggcacc caccggccga ggccttccaa gatggaataa
      121 atggcaatta gcgccagcac catcgggaac atttgcagaa tgagcaggcc gccgagcaag
      181 ccgtcgcgcc cgcgaaaccg catccgggca aacgcatagg cgcacgtggc cgaaagcatc
      241 agaatcagga acgcgcttac cgcggccacc ttgatggagt tccagaacca gcgcagcacc
      301 ggcacggtgg ccgcggccca ggtgccgtcg ggttgcaaca caggcaagcc cagcaccagc
      361 cgccagtgtt cccaactgaa cgtctccgcc gagggcagaa tgccgccggt ggcgaagttg
      421 cccgcgcgca gcgaaatcga aaccaccatc agcagcggca ccagcatcag cgcaatgaat
      481 cccagcagcg ccgcgtgcgc ccagaacagc cgccagcgtt gtcccttgtt catgcgcggc
      541 ctcctcccgc cgcggcgcgc ggccggctgg cgcgcagttg cagccacgcc agcagcgcca
      601 ccactacaaa cagcagcgtg gccacggcgg acgcgaaacc aaaccgcgcc gacgaatcct
      661 tgaacgccat gcggaacgtg tacgacacca gcaaatccgt ggtgccggcc acggtggccg
      721 agcccacgat gtccggcttg ccgccggtca gcagatagat cagcgtgaag ttgttgaagt
      781 tgaacgcaaa actggcaatc agcagtggaa acagcggcgg gaaaatttgc ggcagcgtga
      841 tgcggctgaa gttggtccac ggtccggcgc cgtccatggc cgtggcttcg tacatctcgt
      901 ccggaatggc ctgcaacatt ccggaggcaa taatcatcat gtagggatag cccagccagg
      961 tgttgacaat caggatcatc agccgcgcca gccacggatt ggtaaaccac ttgggtgcaa
     1021 tgccccacaa ggcttgaagc acatggttga tctcgccgta gccctcgtta aataagccct
     1081 tgaacaccag aattgggatg aacgcgggaa tggcgtaggg caggatgagc agcgtgcgat
     1141 aaatgtggcg gccacgcagc gcgggccatt gcagcaggct ggccagcacc acgcccagga
     1201 gaaacgtgat gaccacggag agcagcgcaa acgcaatgtt ccacccgaaa atctggagga
     1261 atggcgcacg gatggccggg tcgcggaaaa tctgccggaa gttttccgga ccaatccaca
     1321 cccgccagcc ggggccgacg gcttcgccag tggtcgcatc cacatagttg ccgcgggccg
     1381 catcggggcg cagggtttga ccgctggcta cgttcaccag cgcctcgcca tcctcacgcc
     1441 agagtggcac gcggtagccc agcgcgcgca ggctgaccag ccgcaacggc accgctttcc
     1501 ccggcgcgga aaagcgcgcg tggttcagcc acggccgtgc ggcaatcacc tcgcgaatgc
     1561 ccactgcggg cgcggtgggc ggcaggatgt tcaaggccag cgtcaccggt tgaccggccg
     1621 cggcttccgc cggcgtgaag gggcgggaga tcagcaggtt gcgaccgatt tcgccgttgc
     1681 ccatgggtac cacaatctgg tagctgccgg gctcggcagc cggatgcaga cggaagttat
     1741 aacgcgcggg atccggtgtg tgccagtcct gcgcgaacca cgcgcggacc tgttcctgcc
     1801 gcagcaaatg ctcaccgcta aaattggtga aggcgatata caccgtgtac actagcggca
     1861 tcagtacaaa gatggcgaac gtggccagcc ccggcagcag gtagcgcgcc gcgtgggtgc
     1921 gccgcgaatt ataaatccac gccgcgccga ggcccgccgc cacaaacacc atgccaagcc
     1981 atgccgcacc ggccaacgcc atttgtacgg ccagccaacc gattcctgcc accacggtca
     2041 gccacagtac ccgtgccaac ccgcgaaaca tcatggcgcc ccccggcgca tccgttgcgc
     2101 ggcgttgtcc agcgcctcct gcggcgtggc ccgtccggcg gtaatggtgg ccagcgccga
     2161 ttccatggaa ctccagaaaa cgcccatctg cggaatgttc ggcatcagca ggcccacctc
     2221 gatattgcgc attgagccgg ccaagcgcgg atcatgagct tgggcttcgt aggccgccat
     2281 caaggccggc acgcccagcg gcacatgccg gtccatggcg gcgagacctt gcggcgtgat
     2341 gaggtaatgc tccaaaaact cctgcgccaa atccagatta gggctggtgc ggttgaacac
     2401 cgcgcccacc acgccgacaa acggccgcgc cggatgccca ttcacgccgg gaatggtcgt
     2461 cacgccaaaa tcaatgccgc tctttttcag gttttcccaa gcgaacggac cggaaatgaa
     2521 catggccagt ttgccctggt tcatctgcgc ctcggcgatg gagtaggaga ggctgcccgg
     2581 cagcaccttg gcgcggatga ggtccgtcac cgccgtcatg gccgccaccg ccgccgggtg
     2641 gttcaccccg atgtccgcaa ggtcatacga accatccggc cgctggccga aaatataacc
     2701 gccatccgcc gccagcagcc cgaacgaaaa ataggcgttg ttgtagtccc acatgatggg
     2761 cgctgcgccg cgcgcctgca ggcgcggcgc cagcttcgcg cagtcggcca agttggtggg
     2821 aatctcgtcc tcaccaatca gcgcacgatt atagatcagc cccggcgact ccatggccag
     2881 cggatagccc cacagtcgcc cgcggtgggt gaaggcctcc cacgccttgg gaaaaatccg
     2941 cgcttgaaat gcagggtccg gatccaccgg cagcagcagc ccgctatccg cccattcgcc
     3001 cacgcggtca tgtgcccaga tcatgatgtc cggccctttg ccgcttttgg ccgcgtggaa
     3061 aaacttgtcg gtggcgccct ccggatgctc gaccaccaca gggatgccgg tgttttgctc
     3121 aaaaatccgg ccgatttccg cgataccctc gtagcccttg tcgccgttga tccataccag
     3181 caactgctgc ggctcccatg ccggcgcgct tagcggcagc gcactcagcg ccaagcacca
     3241 taggatgggg cgaagccacg tcatggcgca caccataccc gcccgcggaa actccgcgca
     3301 acggaaaacc atgtcgccgg gccgactcgc cggcccggtg cgcgtcacgc cgtcggcgca
     3361 gccgccttgc ggcttttgcc cttgagccag ataagcccgc ccacggcaac caccagcagc
     3421 ggaatgccca ccaaagggcg ataggtcagc cacgcaatgg caatccacac cagcgaccac
     3481 gcgccaccca ggacaaggct caccaaggtg atccccgcgc ccacaattgt tcccagcggt
     3541 ggaatcactt tggccagcgc ttccaagatg ccgaaaatgc cgcgcaaacc gccgcacacc
     3601 agcaccacgc caagcaaacg gaacagccag gtgaagatac ggttttcgga ttgcgcgtgc
     3661 gcaaacattt cttcagcgct gaccatgccg acttccaggc gcgaaacggt gcgcccattt
     3721 ttcgccacat actttacgaa ggtgtcgccc tgaacccggc caatcaacga cacctctttg
     3781 ggaggcactt tgctgaacgt gacgcgcaca tcgccgatgg ccggcgtggc gggctcaagg
     3841 ccgaaatata ccgttgagcc atccgtgtgc acccactgcg ctggcggcac agtctcttcg
     3901 tcatcggcat tatcagccgc gggggcgtgg gggttcttgg cgatggtggc cgtccacgcc
     3961 gccgtttgct cagccgttgg ctgaacctgc gcactctccg cgccggtgat ggactccaca
     4021 aaaaagtccg gcaggacata cgcgccgaaa gtcaccgtgt cggcatactg ggtttgctcg
     4081 tcaaaccgga gccatgtcca atttttaccc tgataggccg gatcgtgaaa ctccagcgag
     4141 tcctccggat cggaacacca ctttttctta taggtatagg tggtgatttt ttcctggccg
     4201 ccgcccattt tgtcgcgggt ttccgtcttg gactcctcga cccactggta gtattccacc
     4261 tcgcgggcca gtgcgatggc gttttcgctc acgccaaaag cggaatcact cagtacatcg
     4321 gcggtattgg ccttggccga ggcatggatc aatttgcctt ccagttcggg ggcccgggta
     4381 tccacgctgg ccacggacac cacggcgcct tccgcttccg tcaacgcccg cttcgttttc
     4441 acaaagttgc cctcgttcca gaacagcagc acggtgccga caacaaaagc aatcaagcca
     4501 aagacaattc ccttcaaaga gttgctcaat cgcgaaccgt aggtcgtcgt ggttttttcc
     4561 tgataggcca tggcatcctt tctacacttg gcgtattgtt ccggcgcgtt ctgcccgctc
     4621 gggccaaatc gcgcatggcc gaattttatg cgctttcagc cgggaatacg caagcccatc
     4681 ccggctcccg gccgcgccac ccgcggggtg gaggggcggg ggtggggggg ggcgacgaaa
     4741 aaaatcgccc catctattga catacataca acctttctgg catattgtat aattgcgtcc
     4801 aaaattaccc tagaagcgaa acccttgccc ttatatgtcc agtaagaaaa agaaaacgtc
     4861 cacgtctgcc aagccgaaag ccaaaaagtc cgctaaaccc gccgtcaagc cggccggcaa
     4921 acccgtagcc aagccggcca agaagcctgc cgccaaggtt gccaagcctg tcaagacggc
     4981 ggccaaaccg gtcaaaaccg ccaagcccgc acccaaaccg gccaagtcgg ccaaactggc
     5041 agccaaaacc gccaaggctg ccaagcccgc ggccaaaccg gtttcgaaaa cccaaccaac
     5101 caagaagtca ggcgcgaagc ctgccgtagt cgccaagggc gccaaaattc cggcgcctgc
     5161 ccaagcaagc aagccggtca agggtgcacc acgcggcaaa gccgcccaag gtgcgccacc
     5221 ggtcgctgcg ccgacgcccg ccgccgtgct gtccggcaag aatgccccgg actatatgct
     5281 gcaaatcatt agctacgccc gcgagcgtgg aaacaaaatt tccagcgatg aacttcacga
     5341 gattctgccc cccgaaatca tcggcgtgga agagattgag cgcatcgaag aaaaactgcg
     5401 cagtttgaac atcgaaattt cccgcgatgg tggcgatctc gaaggggcca tccaagccga
     5461 caaggccacc cccgccacgc gcgccgacag ctcggtcatt gatgaccccg tgcgcatgta
     5521 cctcaaacaa atggggcagg taccgctgct cacccgtgag caggaagtcg aaatctccaa
     5581 acgcatcgaa gaagccgaac agcacgccaa gcgcctgctg caccggttcg gctttgccac
     5641 caaggcctat ttcgacatgg tggaccgcct ccagacctcc cgcgaacgct tcgaccgcgt
     5701 gatcgccgac aggccggaag gcgacaaagg agccaagccg gaaaccgaaa aacgcgaaat
     5761 tggccgcgac aaatattttg ggctgattat tcccaaactg accaagagcg tgccgcaaac
     5821 ccaggccgtg gtgcgggaac ggttccgggc gctttccgcc agccgcctga ccatcgccga
     5881 gcaggagcgc cgcaaaaaat tgttcgtcca agcgcgcgag cgcctggcca atatgctcaa
     5941 cgagttctac ttcaagcaga aggccgtgga agatttcgtg gtctacgccg atgagctggc
     6001 ccgctccttc gaaaccctct gcaagcaaat ccgcgatgcc gaaaaaagca gaagcccggc
     6061 caagaaacag catttgcgcg cccacaagaa agccctgcgc gagctggaag aagaacaatg
     6121 cctctgcgtg gaagaattcc tgcagcagca cgccgagctg aaggattggc tgcgcaaggg
     6181 cctgcgcgcc aagtccgaga tggtcgaagc caacctgcgc ctggtgattt ccatcgccaa
     6241 gaagtacacg aaccgcgggc tctcgttcct cgatctgatc caggaaggca acatgggcct
     6301 gatgaaagcc gtcgaaaaat tcgaatatca acgcggctac aagttcagca cctacgccac
     6361 ctggtggatc cggcaggcca tcactcgctc catcgccgat caggcccgca ccatccgcat
     6421 ccccgtgcac atgatcgaga ccatcaacaa acttatgcgc atctccaagc aactggtgca
     6481 aacctatggc cgcgagccca cccccgaaga aatcgccgag gaaatgaacc tgccggtgga
     6541 tcgcgttcgc gcggtcatga aaatgtccca gcagcccatc tccctgcaaa gccccgtcgg
     6601 cgatagcgac gacacccact acggcgactt catcgaggat aaaaccgcgg aaaatcccag
     6661 cgaaatgacc gcctacagtc tcctcaagga gcgcctctcc gatgtcctcg gcaccttgac
     6721 cccgcgcgaa caggaagtat tgacgttgcg ttttggcctg ggagatggtt attctcgcac
     6781 gttggaagag gtcggcaaga agttcaaggt cacccgcgaa cgcattcgcc agatcgaagc
     6841 caaggccctg cggaaaatgc ggcaccccac ccgcattcgc aaattggaag gctttttaga
     6901 aaacgattga aaaaacgacc agaagccctg cccgacaacc cgggaccctg tcccgcaaag
     6961 gagaccccat gagcacaacc atcaagcaga ttgtcaagcg cgacggcaat gttgtcgctt
     7021 acgaccggga tcgaatcgcc accgccatct tcaaggccgc caccgccgtc ggcggcagcg
     7081 atcgcgccga agccgaaaaa ctcgccatgc aagtcgagca gcagttggaa gccaacttca
     7141 gcggcggcac cccctccgtg gaggaaatcc aggatatcgt cgaagaagtc ctgatcaaaa
     7201 acggccacgc caaaacggcc cgcgcctaca tcctctaccg tcacaaccgc gccatggaac
     7261 gcacccagcg cgcctacaag ttcgaagcca ccgacaacat cccctacaag aaaatctacg
     7321 aagttctccg ctggaacatg gaccacggtt gcgaaaccgt agatggcctc aacgacatca
     7381 tcgcccgcgg caagctcccc gaactcatcg ccgcctgcga agaacgctac gacaaggaag
     7441 tccgcgccgg cgccgagaaa ctcatcgagc gcctcccgga agtccgcgtc atgttcattg
     7501 ccggccccag ctcatccagc aaaaccacca ccaccatcaa ggtcagcgaa aaactcgccg
     7561 aagccggcca tgaattcctg gccctgaacg tggaccatta tttcttcaac ctcgagcagc
     7621 accccaagga cgagttcggc gactacgact acgaaacccc gcaggctctc gacctgaagt
     7681 tgattgatga acacgtcgct gcgctcctcg ccggcaaaag catcaagact ccgcactacg
     7741 atttcaaaac cggccagcgc accctgaatg tctacgacat gcagctcgcc cccaatcaga
     7801 ttctgctgat tgactccctc cacgggctct atcgcgacat gacccaaagc accccggaca
     7861 cgaaaaagtt caagctctac atcgagaccc tcggccaagt ccgcaaccgc gaaggcatgt
     7921 tcatgcgctg ggccgacaac cgcctgttgc gccgcatgat ccgcgatagt tggcaccgca
     7981 acttgcagcc cgagcaaacc ctcacccact ggcactacgt gcgccgcagc gaactgcgca
     8041 acatcatccc cttcatcggc accgtggact acctcgtcaa cagcgccatg ccctttgaac
     8101 tgccgatcct caaggcccgg ctcttcaagt atttcccgcc cgccattgag aaattcaaaa
     8161 acgagcccaa gcgccaggac gcctacgtcc gcgcgaagcg cgtctatgaa accctcaagg
     8221 acctcacccc ggtcgaggac gacagccccg tgccgaaaac ctcgctcctg cgcgaattca
     8281 tcggcggcag cacctacaaa tactaatccc cgcccgcggc catcccgccg cgttcgcgtg
     8341 ttttccacgc tgtggaaaat taatttccac agcgtggaac ttttgccgcc gatttgccgc
     8401 ttcgctcgct acggctcgct tgctcgcccg gctgcgccgg tcgctataga gctcgcgttc
     8461 gcccatcgcc ctgcgggctc gggctcggca ctcgcctatt ttccacagtg tggaaaaacc
     8521 ttcgcaaagt tgttccattg tgtggaaaaa tcacgcaaac tttttccatt gcgtggaaaa
     8581 aatccgccgc ccaaacccga atttttccac accgtggaaa acttttttcc acactatgga
     8641 aaactcgccc atgcggggta accatttttc cacactgtgg aaaatctttt cggctgcgcc
     8701 caactttcgt gcctgcaaag cagcggtgga attgggctga gatcagatgg tcgggaaccc
     8761 gatgccctga agatatttga aggtcgggtc gtagtattcc gcggcgcgag ttgggtcgga
     8821 cgggtcacct tcggggacta cgatcaccat gccttgccga gcgcgggtca ataggacgcg
     8881 gtacgcattt tcgaggtatt tctgccgctc cggttgcttc acccgattcc agcgtttgcc
     8941 gacaaatgaa aaatgccgcc actgctcgcc agtcaaccgc aaatcaccat cccacgtaac
     9001 acaaatccaa tccaactcaa ggccctgaat gtcaaactcg gtggcgacat cttccagata
     9061 gtaggaggag cggatgtcgt ccttgcctgc caaaaaccaa tggatgggat tcatcggtgc
     9121 tttcacatgg atgcactgtg gtcgcaggcg ctcggcgctg gaagaggcca ccaagccata
     9181 ccgctcgctg ccccgtgcct gttgccgcag ccactctttg gcgcggtgaa tgttgcgggt
     9241 gaggacaatc ggatagtttg cgctgatctt ttgcaaggtg gcccgggcct ctgcaggatg
     9301 tacatccaga atttctttga ccagttgtga cacatgctcc gctcggtagg agcgcataga
     9361 tacgctcagg tgcaggtcat cgtggtaggt cacatggtct cgttgcgcaa tttccttcaa
     9421 gacccgctgc gcatcatatt ctttatccgt cagtcgcttg gaaatatgaa tgtgccaatt
     9481 tcgaaaacga cgattgagcg cgtctatcca ttctgcgatg ccggcttcac ccgtgttgat
     9541 ctcttggccg ccacccacca ggcaaatcac cactccccag tctgggtggc ggtccacgca
     9601 agaaatcaaa aattccggtt ctgactggtc aaaatccccg tggcccttct tttgcctcat
     9661 gaatttgcag gtttgttgct tattccacgc gcgttgagct tcgtcgaaaa gcgccacatg
     9721 atcggccggc ggcagcggat tgctcacaca atcatcgcga tagtgatgca cgttttgcac
     9781 gaaggcgcga acccggctgt gggcgacgcc tttggtgagc atttggcctt gcgctttagc
     9841 ccggcaaatt tgatcccggg ccagggcctc ttgcaacacc ttgaccaacg ggtcgttgcc
     9901 ggaaagaaat acgctgtata gttgatccgc agcgtcgagg tgtttggtgg caatattgag
     9961 ccccaccaag gttttgcccg cgccgggaac ccccgtcaca aaacaaatgg ccttgtgtcc
    10021 tttggccttg gcgttggcaa taatgccgga aatggcctgg gtggtttcgc gcaaattgat
    10081 cgcaccggca tcgctccgcg agatttccgc cacttggtgt ccccgatata gcgctatggc
    10141 ggcctcaata atggtgggcg tcggccagta gcggccttgc gcccaagttg ccgtctcaag
    10201 cggggtatcc tgaacttcac gcagggtcag cgtaagcaag tcgtgcagcg tctcggcatt
    10261 gcatttgaca ggaacaaaaa ctttatcgtt gtgcacacct gatttgagct gcagcggctg
    10321 agatttggcc gccgtggcca ccaaaatcgg aataatgggg gcgttgtggg aagtctcgtg
    10381 aaagtttttc aaatctagcg cataatccca cacctgatcc atggcatgag cggagtattc
    10441 ggtttcgccg accttgaatt ccaacacgaa caaacaagca ccaatcagca gtacaacatc
    10501 aatgcgcgcc cccatgcgcg ggatggaata ttcaaacaga atctggcccg aaaagccttg
    10561 cagggtagcc ttgagaatag cgatttgttc gcgccaggcg ttgcgctgcg tcaccaagat
    10621 ttcaaactcg gagttctcag ccagtatccc gataaggtga gtggtgtccg tctggagaaa
    10681 ctgctcaatg cgatcggaat aataataccg attcatggtg ccactctaaa tggacactca
    10741 agatccgacc aggaaataaa tgccgacctg tccggcgcgg gtggccggca ggggtgacag
    10801 tcgtttctct tggcagaggg aattaggtgg tgaggtggcg gaggacgtag gggaggatgc
    10861 cgccggcggt gtagtaggcc acttcgatgg gggcatcaat gcggttctgc gcgcggaagc
    10921 gaatttcgct gccgtcggtt ttaaccgcgg tgacttcgtg ccaaccgcgc ggcgccaagg
    10981 ggccttcggc gcgaatggcg tacgtttcgg taccgtcgag gccgaggtct tccgcggttt
    11041 ggccagtcgg aaattccagt ggcaggatgc ccatttccac aaggttggag cggtggatgc
    11101 gctcaaagct ttcggcgatg actgcgcgta cgcccagcag gagggggccc ttggcggccc
    11161 aatcgcgcga gctgccggcg ccatactgct ggccggccaa tacgatgagc ggcgtgccgg
    11221 tttgctggta ggccatggcg gcatcaaaga tgctcatttc gtcgccggcg ggcaggaggc
    11281 gggtccagcc gccttcgcgg cagacgagcc ggttgcgcaa ccgcacgttg gcgaaggtgc
    11341 cgcgcatcat gacttcgtga ttaccgcggc gcgaaccgta ggagttgaaa tccttggggg
    11401 caatgccgcg ttccagcagg tagcgtgcgg cgggggagtc tttggcgatg ctgccggcgg
    11461 gggaaatgtg gtcggtggtg atgaagtcgc caaagtagcc gagcacgcgc gcgccggaaa
    11521 gatcggggtt gggcgggggg gcgtcgagga acgggggctc gcggatatag gtggagtcgg
    11581 gctgccagtc gaagacgggc ttgcggtcca cggcgatgga gttccattcg ggcgtgtatt
    11641 ggttcacgtc ctgataggtt gcggcataga gcgcgggatc gttggcggtg gcgagaagcg
    11701 catcaatttc ggcggcggtg ggccagatgt cccgcagaaa cacgggctgg ccgtcggcgc
    11761 cagtgccgag gggttcttca tcgaggttga tgtccactcg gccggcgagg ccaaaggcca
    11821 tgaccagcgg cggggagcag aggtagttgg ctttgcattg cggatgcacg cggccttcaa
    11881 agttgcggtt gccggagagt acggcggcga caacgaggtc ttggtcgcgc acggcttggg
    11941 cgatatcgtc gcgcaggggg ccgctattgc cgatgcaggt gccgcagccg taggcggcaa
    12001 cgccgaagcc gagcttttcc atttcgggca ggaggccgga ggatttcaaa taggcggtga
    12061 ccacgcgcga accgggaatg agcgaggtct tgacgtgtgg cggcacgcgc aggccgcgcg
    12121 cgacggcttt tttggccagc agtccggcgg cgagcatcag cacggggttg gaggtgttgg
    12181 tgcaggaggt gatggaggcg atggccacgg aaccgtggcg cagctcatcg ccaccggcga
    12241 ggagggcggc ggcttgctgc cgctcggcgg gcacgccgaa gccgcccttt tccggcgcgg
    12301 cacccagcgc ggcgaggaag cgccttttga tttcgccgat aggttggaaa tcgtgcgggc
    12361 gtttcgggcc ggcgatggcg gagacaacgg tgccgaggtc cagttcgatc acgcgcgaat
    12421 acgccggctc gggggagtct ggctcctgga acaggccttg gcgacgggca tattcctcga
    12481 ccagttcgca atgcgcggcg gggcggccgg tcatgcgcag gtaggcgatg gtttcggcgt
    12541 tcatggggaa gaacccggtg gtggcgccat attcgggggc catgttcgag agcacggcgc
    12601 ggtcggagag cgagaggctc gcgcagccgg ggccgaagta ttccacaaac tggcccacga
    12661 cattatgttg acggagcagt tgggtgatgg tcagcgcgag atcggtggcg gtgacgccgg
    12721 ggcgtagcgc gccgctcaag cgcacgccgg tcaccaccgg ggtcagcagg gcgatgggtt
    12781 ggccgagcat ggcggcttcg gcttcgatgc cgcccacgcc ccagcccagt acgccgaggc
    12841 cgttgaccat gggcgtgtgg gagtcggtgc cgacgagaga gtcgggatag gccagccagg
    12901 tgccatcggc ttgttcggcc acgtggatgc catcggccag aaattccaga ttcacctgat
    12961 ggcagatgcc gacgccgggg ggcacgatgc gcagtttttt gaacgcttgc tggccccact
    13021 tcaaaaattc gtagcgttcg cggttgcggc tgaattcgat ttcgatgttc tggttgagcg
    13081 catcgggcat ggcccagcaa tcaatctgca cggagtggtc cacgaccaaa tccacgggga
    13141 cgaggggttc gatgatgccc gggtcgccgc cgttgcgcac cacggcgctg cggaggctgg
    13201 cgaggtccac cacacagggc acgccggtga ggtcttgcag caggacgcgc gcggggagat
    13261 aggggatttc ttcctgcgcg gcggtctggc ccggccgcca gcgcgccagc gcttccacct
    13321 gccgccagtt gtaggcgggg tggccctgat tgcgcacggc ggattccaag agaatgcgca
    13381 cgatataggg caactcggcg agggaggaac cgcgcagttc ggtcagcgcg gccaggctgt
    13441 ggtaggccac ggtccggccg ttgtgggtga acgtcttcaa ggtggcgatg ggttgggaca
    13501 tctggctttc ctccgtaaac atgtgtccgc caaaatatcg tgtgtgagcg gcgggggcaa
    13561 ccccatttgc catggcaaaa aaaaaccgcc ttgcggcggg ggtgtccggg atttggtgga
    13621 gggtaggagg ttcgaactcc cgacctcctg aatgccattc aggcgctcta ccaactgagc
    13681 taacccccca aatcctcaga cgatgacagg cttgtaccac gcgccggggg tggagtcaac
    13741 ccacccgggc aaaaaacttt tgcggcgagg tgttgacatt gccggaggct ttgcgtacgg
    13801 tgtcggcctg ttttgtggaa caggggggga taggccccct ccagaaacga ggattcagat
    13861 ggaagcgtat gcagtgattg agaccggtgg caagcagtac cgggtccaga aagacgatgt
    13921 gttgtccgtg gaactgctgg cggatgccaa ggttggccag cagatcgagc tgggccgcgt
    13981 gctggcgttc aacgatggca gcggactcaa gattggcgcc ccggaagtgg ccggcgtgaa
    14041 ggtggccgcg gaagtggtgg aagaataccg cgcggcgaag gtcgtggcgt tcaagaagaa
    14101 gcgccgcaag ggctatcacc gcaaagtggg gcaccgccag agcctgaaca aagtcaagat
    14161 tgcatccatc gcatagggaa ggaggccgga aatggcacat aaaaagggac aaggcagcag
    14221 ccgcaacggg cgtgacagcc acggccagcg gcgcggaatc aagcgctttg gcggccagac
    14281 cgtcaccgcc ggcagcgtgc tggtgcggca gtgcggcacc aagaagaccc ccggactgaa
    14341 cgtgggcatg ggcaaggact acaccctgtt tgcgctggtg gatggcgtgg tgtcattcga
    14401 ccgcggcggc aagcgggtca acgtgcaaaa actggcccaa gcctaagacg cgttgcgcgc
    14461 cggattgacg caaggggcgg cccgcaaggc gccgcccttt tgctttgggg cattatgaaa
    14521 actctgacgt ttgtggatcg cgtgcgcatt caagttttcg ccggcaatgg cgggaacggg
    14581 gtggcgttgt tccgccgcga aaaattcatt ccgcacggcg ggccggatgg cggagatggc
    14641 ggcaacggcg gcagcgtatt tttgcgggcc tcgaaggatg aggcctcgct gttggatttg
    14701 cactttgcgc cgctggtgcg cgcggagcat ggcgagcatg ggcggggcaa gcagcaatat
    14761 ggtcgcaacg gacaggataa aatcgtgccc gtgcccttgg gcaccgcagt gttcgatgcc
    14821 gaaaccgagg aactggtcgg cgaagtgctg caagatggcg acctgctgaa agtggcccaa
    14881 ggcggcaagg gcgggtgggg caacatccat ttcaaaacca gcacgcatca ggcgccaacg
    14941 gaattcaccg aaggcacacc cggcgaaatc cgtacgctgc ggctggtgat gaagaccgtg
    15001 gcggaggtcg gcctggtggg ctatcccaat gcgggcaaat ccacgctgtt gacgcagttg
    15061 agcgccgcgc ggcccaaggt ggcggcctat ccgttcacca cgctgcatcc gcaggtgggc
    15121 attttgaagt tcgatgccac ccacgccatc cgcgtggctg atgtgcccgg cctgatcaaa
    15181 ggcgcgcatc agggcgtggg gctcggtcat gattttttgc ggcacatcga gcgtaccgca
    15241 tttctgttgt tcgtgattga catggccggc gtagatggcc gcgatccgac ggcggacttc
    15301 cgcagtctgc gcgaggaact caaactgtat aatccggagc tggccgcccg gccctatttg
    15361 gtattggcca ataaaatgga tgagccggcc gcgcaaaaaa atctggccgc cttcaagcgc
    15421 aagacaaagg aaaaaccgtt gcccatgtcc gccgagttgg gcgagggggt gccggagctc
    15481 aaggcgcggc tgtttgccca attctttccc ggcgaaacca tcttggatat ctagaccgct
    15541 gctttccgct tgctttcccg ccgccgttca cgcagggtgg cgcgcctatg gaaaccaacc
    15601 cgcccccctc tgattccttc aacgagttcg gctatcgcaa aaaacgtccc gcccgcgcgg
    15661 ctgatgatgc tccgcggggc ccgcgcccct tccgcaagcc ttatcgtgct ggccgcgatg
    15721 acgcgccgcc cgtcgcagaa ggcgaaggcc aagatttccg ccggccgtat cggccgccgt
    15781 atcgagacca agaccgcccg ccacggccct atccgcgccg cgacgagcgt ggcgcacatg
    15841 cgccgggcgg cttccgcaag ccgtaccgcc cgcgccatga tgatggccaa ccgccgcgcg
    15901 atggcgcggg gcaggatttt cgccgtccgc gccgcgagga tgatcgtccg cgcgggccgg
    15961 ggggctatcg caaaccctat cggccccgca ccacggagcc gggcgatcag gagtcgtcgg
    16021 cggggtaccg tccgcggcgc ccgggtggcg ggcattttcg caagccgtat ggcgggccgc
    16081 gttcagacgg accgggcggc tcctccgggc cgcgcggcgg ctacggcaga ccctatggcc
    16141 ggcccgcagc gggcgagggc caagagcgcg gttttcgcaa accctactct ccgcgcccgg
    16201 gcggctataa aaagccctat ggcccaccac gcggtgaggg cgcgggcggt tatcgcaagc
    16261 cttatggccc accgcgcagc gaaggggaag gcggctacaa gaagccatac gggccgccac
    16321 gcggctcgcg ccccggcggc ggttttcgta aaccttatgg cccgccgcgc ggtgaaggtg
    16381 agggcggtta tcgcaaacct tatggaccgt cgcgcggccc gcgccccggc ggtggttttc
    16441 gtaagccata cgaacgcggt cccggtgggc cgcgtaaacc ttattccggc ccacgcaagc
    16501 cgtatggcga tggccaacgg cccacgtggc gcaagaacga aggcgcagac gatgcgccac
    16561 cgcaggcctg aaccgcatct ggcggcgata acctgtccat gaaaccgatt gaccgcaccg
    16621 cgggcggcgt ttagagtagc ccggtttttg tcactgaact tgtagggaga gaatggcgat
    16681 ggcgaaaaaa gaagaacagc aaaccgcaaa cggcgtttcg gccaagagcg cgttgatttt
    16741 tgagatggat aatattgctc tcggcgcacg ccggattcgt tacgaggtgt tggcgggcat
    16801 cctgaaagag gaaaaaatcg gactgtcgcc cgtgcagttc tcgcgctatt gcctgcaccc
    16861 ggcgccgtcg gtttatatgg agtcgtttct ggaaacgatg aaatacaagt cggccacgcc
    16921 ggagcaagtg gtggaccggc tgaccaacga gacggtttcc catctgatgc aaaaatccac
    16981 caccatcaac ccggggttga tcaagtggat tgaagccgcc caggcacgcg gcgcggctgt
    17041 tgcctgcatt tccatgctgc cgcaagcggc ggcggaaggc gtagccgccc atctgcactt
    17101 cgaacgctgg ggcatcaagg tgttttccgc cacgcccacg gacaagggat tcccgcacgc
    17161 caagaactgg ctgcaaatgg ccaaggccat cgcccgccat ccgcggcgct gtctcgccat
    17221 cgtgagcagc atgaccgtgg ccaaaaccgc gctggcggcc atgatgaaca ttgtggtcgt
    17281 gcccgacgaa ttcacggaat accaggattt ctgcggcgcg aatctggtgt gtgcggacct
    17341 gaaagatgtg aatcccgatg agtacttcga gcaaaccaag ttctaaacgc gccgccgccc
    17401 gcgcccaaaa gtcccgctca tctgtccgcc gagcctcagc caaggcttcg ccccgtgcat
    17461 tggcgcgtgg cgcggaccaa atggcgcggc tgctgaaaat catggcccgc ctgcggcggc
    17521 ccggcgatgg ctgtccgtgg gacctcgaac agaccattga gagcatcaag ccgaacctga
    17581 ttgaagaagc ctacgaggcg ctcgacgcca tggaaagtgg caaccgcgtt gatctagccg
    17641 aagaactggg cgacttgctg ctgcagatta ttttccaggc gcagattgcc gctgaagagg
    17701 gcaccttcaa ctttgcggat gtggcgcagg gtatttctga caagctggtc cggcgacacc
    17761 cccatatatt tggcgatgtc aaagtgcgcg actccaagga tgttttgcgc aactgggatg
    17821 ccatcaagaa aaccgaaaag aaggtgcgca agtccgcgtt ggatggcgtg cccaagcatg
    17881 tgccgccgct ccaccgtgcc tatcaactgc aacggcgcgc ggcgcgggtc aactttgatt
    17941 ggcccgatat tgctgatgtc ttcaaaaagc tggacgagga agtccgcgag ttgaaaaccg
    18001 ccgtcaaccg caaacagcgg cagggcatca ttgaagaatt gggcgacctg ctcttttccg
    18061 tggtcaatgt ggcccgcttc atgaaggtgg acccggccta tgcgctggag cgcaccaacg
    18121 ccaagtttgt gcgccgcttc gaagtcgtgg aaaaggaaat taccgcttcc ggccgcaagc
    18181 tgaaggattg cacccttgct gaaatggatg ccatctggaa ccgcgtacgc gcggccgaca
    18241 agcgcgcgta aaaccggctg gctggcactg gggctggccg gactgctggc catggccgca
    18301 cccgccgcgg aaccgccgct ggcgcggttg gcgatctggt gtgaattcat gccctatgcc
    18361 gaagtcgcgc gcgagctgcc gactttggcc gaatatgatt gtgatctgat tctccacgtg
    18421 gagcgcgaaa gtttcgccga cccagatttt gcgccgctgg tgcgcgcggc ggcgcaagcc
    18481 ggggtgcggg tggatgcctg gctgctgctg ccctatgacg agcacctgta tgtggggcag
    18541 gcgagtttgg ccgccacgcg agatctggcc cggcaagccg tggagtattt tactcaagaa
    18601 aaactcgcgg tgcgcgcttt tgtgtttgat tgcgagccgt caccgctgtt ggggcgcgaa
    18661 ttgttcgccg cggtgcgggc aaagagtccg cgccagttgg caggcgtgct gcgcgcccag
    18721 ttgaatccca ctcaattttt gcgagacgtg gctgagctca atgagttgat tgccgggttg
    18781 cacgccagcg gttacgccgt ggatggcgcg gccaaccgcg cgtttctcga tgcgcaggcg
    18841 cgcggcaacg tggcgctgga ggatgcgctc aacgcgccgg tgaccggtgt gacatgggac
    18901 gcgctgagct tcattaccta ccgctaccgg gttggccagg aaagttatgt ggcgatgctg
    18961 aaccgctacg ccactctggc ccggcggctc tatggcgtgc gagcagcggt ggatgtgggt
    19021 ttgatcggcg actacaccgc cattccgcaa aacgcggagc gggcggcatt gttcggcggc
    19081 ggcgcagatt tttatgcgta tttgcgcggc atgcgcgata cgcgcgatct cgaacaggcg
    19141 gtgggcgtgg tgctggggtg cggcgtgcgg cgtgtgaatc tctacgcgct cgacggcgcg
    19201 gcgacttccg tggccggcac ggaatcctgg ctgcgcgcgg cgcggcaggc gcgaccccgg
    19261 ggcgcgtggg cgcgctggac cccactgggt tccctgcaat atggcgcgct cggcgcgctg
    19321 actgaaaaaa tgttccggtg gaccaccggc ggcacaggaa aagcgtatga aaaaaaagaa
    19381 aaaacagaat cgtaaattga tcttggccag cgccagccca cggcggcgtg aatatttccg
    19441 gcaactgggc tatcccttcc gggtggcgac gccgctcacg caggagcggc cacggcgcgg
    19501 cgaagatgcg gcggaatatg tcctgcgcaa cgcgcaggaa aaggccatgg atgtggcgca
    19561 gcgggtctcg cccgaggccg tgattgtggc ggcagatacc gtggtggtgt tacgtggaaa
    19621 aattatggaa aaaccgcagt cggcgcgcgg cgcgcaggcg atgttgcggg cgctcagcgg
    19681 ccaggtgcat caggtcatca ccggtttttg cgtgtatgag ccggggcgca aggggccact
    19741 gaaatcgtgg gcgcaggccg ttgtgaccga tgtggagttc aaaaagctca gcgccgccga
    19801 aatcaaggcc tatatcgaaa gcggggagcc gatggacaag gcgggggcct atgccattca
    19861 gggccgcgcg gcctacatga tccggcaaat ccaaggctcc tacacgaacg tggtgggcct
    19921 gcctatgacc gagctggtgg aggtgctcac cggcgagttt gggctgcacc cgacctttgc
    19981 cggtccggct cgcgcgtagc ggcgcgggtc agtcgtccgc cgcgaggatg cggatgggaa
    20041 cgggcaggac gaatatccga ccgggtttct tattgctcat cccatagatg cgctggcctt
    20101 tttccgtacg cagcaaaacg gaaagcacgt agtcgccggg cgcaaagtgt tcaggaagtt
    20161 ggatggtttg ctccagcgtg tagatgccgg gatggatttg acattgcagg tcattgagat
    20221 cgcatacggc ggcgatgata ctgcgttctt ccacccattt gccgctggcg gcagtcaccc
    20281 gggttgccaa caccgaaccg gtgatgattt ccaaatcacg cgggcattgg aggtaatagc
    20341 gcaggcggaa ggcgccgccg gccggcgcgg tggtttggag cggttcacaa ccgataaggc
    20401 gaaacccgct cgtgaaacgg attttcagcg gaatggcttc ggcggggatt tggtgaatgt
    20461 ggccgagcgg caggggacag ttggcagtgg cgcaatccag cgccggttcc gtgagtgggc
    20521 cggggtggcg cgcggccgcg aagctgtcga ggaagaaggg catggcgtct tctgtgcgga
    20581 atcggaacag gttgggcgtg agtttgacgg cgtaacgagc gggaatatcc acatggatat
    20641 cacgtgcttc cggtccaatg gtccagcgtc cgataacata gccgttcaag tccagccaga
    20701 gcgtgacggg tgggctgccg gccggagttc gcgcgctgaa agtgatgcag ggatactgga
    20761 caagataatc gtggcgcacc atgcggtggg cctcggcggc tggttccgtg ggtgggttca
    20821 atttgagcaa cgcaaaacgg ttatcttcgg caaccaggct ggcttcgccc gcaaacagct
    20881 cgacataatt gtatggaatg cgagctttac gcgtgacgct cttatcttcc aaaatgtaac
    20941 aggggggcca cacctggcgc aggagattat tgagtcttgc atatataagt gacattatgt
    21001 cacaaagaaa gctcggcttg
//
LOCUS       DAOPNP010000035        20756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3163_length_20756_cov_9.371238, whole genome shotgun sequence.
ACCESSION   DAOPNP010000035 DAOPNP010000000
VERSION     DAOPNP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3163_length_20756_cov_9.371238"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PKI64_04675"
     CDS             complement(<1..911)
                     /locus_tag="PKI64_04675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOE00340.1"
                     /translation="MTTRLNRFEMPKFVVKDEATATSTYAQFIAEPFEAGYGRTLGNS
                     LRRVLLSSLEGAAITSVRIQGAEHEFCHLKGCVEDVTDIILNLKKVLFKLYTRQPRTF
                     RIKVKGAGDVTARDIQCDNTAEVLNPDQHICTLDGDGVFDAEVEVKIGRGYCPADWNK
                     QPDQEIGLIPIDSLFSPVRRVKYTVDDTRVGRRTDYDKLTLEIWTDGRISPDEALTLS
                     AAILRHHLDVFVNFDKDIAEFQESEKAVDPAREELARKLAISVNEIELSVRAANCLNN
                     ANITTVGELAQKTENEMLKYRNFGKKSL"
     gene            complement(973..1599)
                     /gene="rpsD"
                     /locus_tag="PKI64_04680"
     CDS             complement(973..1599)
                     /gene="rpsD"
                     /locus_tag="PKI64_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOE00341.1"
                     /translation="MGRYIGPACKRCRRLGVKLFLKGDRCYMAKCPIEQGHSIPGMHG
                     GRRPRLKDYGLQLQEKQRLRNMYGMQEGQFRLFFQRALRSRGVTGEVLLQALEMRLDN
                     LVYRIGFAPSRRSARQFVRHGHIRVNGQKVNIPSYKCKPGDVIQAREAKRSRDYAKAH
                     LDAAESRGWPVWLNVDKAEHKAEILHVPSREEIAPIVNEQLIVELYSK"
     gene            complement(1627..2100)
                     /gene="rpsK"
                     /locus_tag="PKI64_04685"
     CDS             complement(1627..2100)
                     /gene="rpsK"
                     /locus_tag="PKI64_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOE00342.1"
                     /translation="MAEEIKDEVKSAPAPAAAEAAPAAETPAAEGEVKRRRVKGAKNV
                     PVGVVHILASFNNTTVSITDPRGNVISWSSGGRCGFKGSRKSTAYAGTVVAQEACKVA
                     VGFGMHEVEVRVQGPGSGRESAIRAVQAAGLTITGIKDITPVPHNGCRARKRRRV"
     gene            complement(2111..2521)
                     /gene="rpsM"
                     /locus_tag="PKI64_04690"
     CDS             complement(2111..2521)
                     /gene="rpsM"
                     /locus_tag="PKI64_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOE00343.1"
                     /translation="MPRVIGVDIPGKKRVEYALQYIYGVGPTRAKELVARLKIDPAKK
                     ADDLTGEEIATLSKMLQEEYVVEGDLRRGVAGNIRRLIAVNCYRGIRHRKGLPVRGQR
                     TSTNARTRKGPRKTVGAVRGKEARSAAKSAPAAK"
     gene            complement(2541..2654)
                     /gene="rpmJ"
                     /locus_tag="PKI64_04695"
     CDS             complement(2541..2654)
                     /gene="rpmJ"
                     /locus_tag="PKI64_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOE00344.1"
                     /translation="MKVRTSVKRICERCKIVRRKGVVRVICTDLRHKQRQG"
     gene            complement(2664..2795)
                     /locus_tag="PKI64_04700"
     CDS             complement(2664..2795)
                     /locus_tag="PKI64_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00345.1"
                     /translation="MAFVPREHAPATGGLCPAVGARLRVRMSPFDMSRGEIVEIVES"
     gene            complement(2873..3631)
                     /gene="map"
                     /locus_tag="PKI64_04705"
     CDS             complement(2873..3631)
                     /gene="map"
                     /locus_tag="PKI64_04705"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOE00346.1"
                     /translation="MIPIKTRAELASMRVACRLSAQVLAEMAAQVVPGITTGELDEFA
                     ARRMRELGVKSAFFGYGGFPGHTCISLNDEVVHGIPGRRVINAGDLVSVDLGIVAEGF
                     IGDNAITVRVGAVDEESDRLCRVGEAALAAGIAAAQAGNRVGDIGHAVQAVTEAAGFS
                     VVRDFCGHGVGRKLHEDPQIPNYGEPGRGPRLQPGMTLAIEPMINSGTWQVEVLPNRW
                     TVRTRDRRRSAHYEHTVLVVEQGPAEILTCVAAK"
     gene            complement(3642..4286)
                     /locus_tag="PKI64_04710"
     CDS             complement(3642..4286)
                     /locus_tag="PKI64_04710"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOE00347.1"
                     /translation="MQAIILLGAPGAGKGTLAEGVRNATDYIHVSTGDMLRAAIKAGS
                     QTGLEAKAFMDKGELVPDSVIMRIVSERLAQGQPTDKYMFDGFPRTLEQARQLDEVLA
                     KLGSAVNQVFLLEVPTPVIVSRLSGRRICKTCGAVYHVTNIPPKKEGICDQCGGPLYQ
                     RPDDEEATVLNRLDVYQRQTASLIDFYDQKGILIRINAGTNPQAATAELLAKLA"
     gene            complement(4310..5662)
                     /gene="secY"
                     /locus_tag="PKI64_04715"
     CDS             complement(4310..5662)
                     /gene="secY"
                     /locus_tag="PKI64_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOE00348.1"
                     /translation="MLSAFSNSLKIPELRQRIFFTLALIFVCRLLSVIPVPGVDTEML
                     RGIMDRMASQSGNMGFMGLLDLFSGGALTQAAIGALGIMPSISASIILQLLTAVVPAL
                     EKLAREGDAGRQQIIQYGRYLTVALCLVQGYAMAAVLLRPEALGLQIMPGETIVTMSR
                     VPFLLLAILTMTTGTMTLMWLGEQITARGIGNGISLIITISIVSRLPGAISQAVAMFR
                     TGSANIFMLVAMLALAFLVIAGVIAVTQAQRKIPVQYAKRVVGRKTYGGQSTYMPLRV
                     NYSGVMPIIFAQAILMFPQRLLMMIPIDAVKNFAAVHLRYAGPTYITLYGLMILFFSY
                     FWVATQFHPVQIADDLKKYGGYVPGIRPGKPTAEFLDRVMTRITLAGAVFLTIIAVLP
                     MILGNSFHIPWMVAQFFGGTSLLIIVGVMLDTMRQVESHLVTRHYDGFLRKGQLRGRR
                     "
     gene            complement(5689..6126)
                     /gene="rplO"
                     /locus_tag="PKI64_04720"
     CDS             complement(5689..6126)
                     /gene="rplO"
                     /locus_tag="PKI64_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOE00349.1"
                     /translation="MNLHSLKNTPGAKKRRMRVGRGESSGKGRTAGKGNKGQMSRTGH
                     KHKPLFEGGQMPLIRRVPKRGFTSPNRVEYAALNIGELAKLLPGEITPEALVAAGRVS
                     PKSRIKVLGGGEITAARQVKAHAFSATAKAKIEQAGGTCEVIV"
     gene            complement(6149..6649)
                     /gene="rpsE"
                     /locus_tag="PKI64_04725"
     CDS             complement(6149..6649)
                     /gene="rpsE"
                     /locus_tag="PKI64_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOE00350.1"
                     /translation="MKREAKKNEEFQQKSPAEALERVVEVNRCAKVVKGGRRFSFSAL
                     VVVGDRNGQVGYALGKARENADAIRKATEAARRNMVKVTMLDRTIPHEVIGRYGASRV
                     MLRPATKGTGVIAGGGARAVLDLAGIRDVLAKSLGSGSALNVVKATMNGLQQLRSREE
                     TAALRQ"
     gene            complement(6679..7035)
                     /gene="rplR"
                     /locus_tag="PKI64_04730"
     CDS             complement(6679..7035)
                     /gene="rplR"
                     /locus_tag="PKI64_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOE00351.1"
                     /translation="MRMKTLKDYRVRRHRRLRQKVQGTAERPRMSVFVSNVHMYVQFI
                     DDLAGKTLAAVSTLAGPVKGQKLNVATAKQLGALAAQVAKEKGIAEVVFDRGGFAYRG
                     RLQALADAAREGGLKF"
     gene            complement(7038..7574)
                     /gene="rplF"
                     /locus_tag="PKI64_04735"
     CDS             complement(7038..7574)
                     /gene="rplF"
                     /locus_tag="PKI64_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOE00352.1"
                     /translation="MSRIGKLPVVLPAGVTAQVAGDAVTLKGPKGELTQKFPDYINVV
                     QEGDKLLVSRTDDSKQSRAGHGTIRALLANMAVGVKDGYSRELEIQGVGFKASIAGKQ
                     LTLNVGYSHPVEYTVPEGITVTVTDGTQLKVSGVDKQLVGQVSARIRSFRPPEPYKGK
                     GVRYKDERVRRKAGKTVA"
     gene            complement(7594..7992)
                     /gene="rpsH"
                     /locus_tag="PKI64_04740"
     CDS             complement(7594..7992)
                     /gene="rpsH"
                     /locus_tag="PKI64_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOE00353.1"
                     /translation="MSCSDPIADMLTCIRNASRAGLPATEMGHSRLKAEVARILKKEG
                     YIADVATAERDGKKVLRVTLKYDYNEKPIIQQLRRVSRPGLRRYVGAAEIPRVRGGMG
                     TAVLSTSRGVMSDREARAARVGGEVLCQVW"
     gene            complement(8008..8193)
                     /locus_tag="PKI64_04745"
     CDS             complement(8008..8193)
                     /locus_tag="PKI64_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011206038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOE00354.1"
                     /translation="MAKISWMVKASREPKFAVRGYSRCRRCGRARAYIRKFQLCRICF
                     RELASEGKIPGVVKASW"
     gene            complement(8218..8754)
                     /gene="rplE"
                     /locus_tag="PKI64_04750"
     CDS             complement(8218..8754)
                     /gene="rplE"
                     /locus_tag="PKI64_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOE00355.1"
                     /translation="MSQLKEKYQKDVVKALKATGKYPSTMDVPRVVKVVVNMSVNALV
                     DRDMLKAVSDDLAKITGQRPTQRKATKSISNFKLREGMPIGAKVTLRGRRMYEFLERL
                     IHATLPRIRDFRGVPAKAFDGRGNYTLGLKEQTLFPEIESDKVKRVQGMDITIVTTAQ
                     NNADAKELLQLLGMPFAT"
     gene            complement(8759..9025)
                     /gene="rplX"
                     /locus_tag="PKI64_04755"
     CDS             complement(8759..9025)
                     /gene="rplX"
                     /locus_tag="PKI64_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOE00356.1"
                     /translation="MKKPKMHVRRDDIVRAISGEDADGKKTGKVLRVYPKTGRVLVEG
                     FNFVKKHLRKSQDNPNGGIVTREAPMAASKLVVVTRGGKKTGKE"
     gene            complement(9025..9390)
                     /gene="rplN"
                     /locus_tag="PKI64_04760"
     CDS             complement(9025..9390)
                     /gene="rplN"
                     /locus_tag="PKI64_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOE00357.1"
                     /translation="MIQMESLLPVADNTGARTAKCIKVLGQRKTVAGIGDIIRVSIRE
                     AQPAGMVKKGDKALALIVRTAAPIHRPDGSTLSFDNNAVILIDEQKNPRGSRIFGPVA
                     RELRELNFMKVISLAPEVL"
     gene            complement(9393..9668)
                     /gene="rpsQ"
                     /locus_tag="PKI64_04765"
     CDS             complement(9393..9668)
                     /gene="rpsQ"
                     /locus_tag="PKI64_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOE00358.1"
                     /translation="MATETRNLRKERMGVVVSDAMNKTVVVQAERRIAHPVYGKILRR
                     SKKYYAHDEQNEARRGDQVLIAETRPLSKLKRWRLVRVVTRAAKSEV"
     gene            complement(9675..9869)
                     /gene="rpmC"
                     /locus_tag="PKI64_04770"
     CDS             complement(9675..9869)
                     /gene="rpmC"
                     /locus_tag="PKI64_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOE00359.1"
                     /translation="MKARDIRDLSPEERLAKLAEMEKELFNLRIQQTAGQLEKPDRVR
                     LVRRDIARIKTVINQAQAAR"
     gene            complement(9890..10309)
                     /gene="rplP"
                     /locus_tag="PKI64_04775"
     CDS             complement(9890..10309)
                     /gene="rplP"
                     /locus_tag="PKI64_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOE00360.1"
                     /translation="MPLMPKRVKYRQSQRGSRKGMAQSGNSLAFGEYGLRAMERCWLT
                     NIQIEAARVAMNRAMKRKGKLWIRVFPDKSYSKKPLEVRMGKGKGAIEGWVAVIRPGR
                     ILFEMDGVSEAMALECLRLAATKLPIRTTFVRRGVVL"
     gene            complement(10293..10970)
                     /gene="rpsC"
                     /locus_tag="PKI64_04780"
     CDS             complement(10293..10970)
                     /gene="rpsC"
                     /locus_tag="PKI64_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOE00361.1"
                     /translation="MGQKVNPIGFRTAVHKQWKSRWFAERKEFGNLLNEDLEIRDLVR
                     TRLKDAAVADIVIERYAQRARVTVFTARPGVVIGRKGEGIDKLREELSAKTGKEIYVE
                     IKEVRNPDTNAQLVAESIALQLERRVAFRRAMKRALQLTMDMGALGIRVQACGRLGGA
                     ELARTEHYMVGKVPLQTLRADVQYGFAEANTMAGKIGVKCWICVKPEDAAAKSVTNKE
                     KSYAPYA"
     gene            complement(10992..11342)
                     /gene="rplV"
                     /locus_tag="PKI64_04785"
     CDS             complement(10992..11342)
                     /gene="rplV"
                     /locus_tag="PKI64_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOE00362.1"
                     /translation="MEIIAVTKYVRLTPTKARDLTRAITGKSVGEALQVVELNKRKAA
                     VAIGKTLKSAIANAENNAELSADKLYVARAVIENGPTLRRYWARARGSASPIRKKTAH
                     VRITLTDKKPRKKK"
     gene            complement(11353..12105)
                     /locus_tag="PKI64_04790"
     CDS             complement(11353..12105)
                     /locus_tag="PKI64_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00363.1"
                     /translation="MMGFRRGWRLIWAVPVVGAALAAGWWFGTRQAQNSSLTTGPASA
                     PARLEMEPGPVPEAPVEAETAELSVQSAQDEANIPIRTDGPAAAAMGGAADGHHDEVL
                     DLVTSEARLVTVRLAQENAEMRRAVAELTRRIRQLSTALAEAQAALDVQEARRAAAAG
                     APAVEPLDAPREVRLVDVNAELGLVILDQGARQGVRYGLPLTVLRDRRRVARIRVVDV
                     RERIAGAAVEETARGDYPQTGDRAVLIRPEGP"
     gene            complement(12117..12401)
                     /gene="rpsS"
                     /locus_tag="PKI64_04795"
     CDS             complement(12117..12401)
                     /gene="rpsS"
                     /locus_tag="PKI64_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOE00364.1"
                     /translation="MTRSIKKGPYVEDHLLKKVEALNASGAKRPIKTWSRRSMILPEF
                     VGHTFTVHNGKVFTSVFVTENMVGHRLGEFAPTRTFRKHGAATDKTIALK"
     gene            complement(12403..13233)
                     /gene="rplB"
                     /locus_tag="PKI64_04800"
     CDS             complement(12403..13233)
                     /gene="rplB"
                     /locus_tag="PKI64_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOE00365.1"
                     /translation="MGLKKYKPATAALRFTTLSDFEGLSKKRPERRLIKIKKSKAGRN
                     AAGRITVRHRGGGHKKFLRQVDFMRHDKRDIPAKVQALEYDPNRTARLALLAYADGEK
                     RYILAPNGLQVGATVMAGPEAEPTDGNALPLASIPIGMPIHALELTPGAGAKMVRSAG
                     MVARVTARDAGYVQVKLPSGELRKVRGECYATIGQVGNLEHENISLGKAGRKRWKGIR
                     PTVRGVAMNPVDHPMGGGEGRTSGGGHPVTPWGQLTKGKKTRSGRKPSSKFIVERRKK
                     "
     gene            complement(13247..13525)
                     /gene="rplW"
                     /locus_tag="PKI64_04805"
     CDS             complement(13247..13525)
                     /gene="rplW"
                     /locus_tag="PKI64_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOE00366.1"
                     /translation="MKNLIVKKLLVTEKGTRLKETGNQYIFAVARAANKLEIRREVEQ
                     RFNVKVKDVNTMVRPGKAKRLRTAAYGRTPDWKRAVVTLQAGHTIEMA"
     gene            complement(13529..14164)
                     /gene="rplD"
                     /locus_tag="PKI64_04810"
     CDS             complement(13529..14164)
                     /gene="rplD"
                     /locus_tag="PKI64_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOE00367.1"
                     /translation="MSKIAIYNLNGEAAGEQEFAADLLETKRGGQAVLDVVTAYRAGL
                     RKGTASTKKRGEVSGGGRKPFKQKGSGRARQGSIRSPLLKGGGVVFGPHPRSYDKKVN
                     KKVVALAFRRALSEKIKAGGVVVANGLESVEGKTKALAGLLQKAAGKQATLFVTGAPN
                     QNLVRAARNMPRVEVTTAAQANTYQIMRYPVIVTDAAGLTALQARLAEGAK"
     gene            complement(14173..14802)
                     /gene="rplC"
                     /locus_tag="PKI64_04815"
     CDS             complement(14173..14802)
                     /gene="rplC"
                     /locus_tag="PKI64_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOE00368.1"
                     /translation="MQALIGRKIGMTRVYDDAGVQVPVTVVQVGPCTVVQRKSAQPDG
                     YEAVQLGFEEQKESRLTKPQQGHFKKAGAKPAKVLREVRLAAGEEAKAGDVLTAKIFE
                     GVKFVDVLGHTKGRGFQGVVKRFKLGGGRASHGGGWMRRPGSIGGREEPGRIFRNKKL
                     PGHMGSVNVTTQNLRVVKVLADDHALLLEGAVPGPVGGIVLVRKAIKKA"
     gene            complement(14817..15125)
                     /gene="rpsJ"
                     /locus_tag="PKI64_04820"
     CDS             complement(14817..15125)
                     /gene="rpsJ"
                     /locus_tag="PKI64_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOE00369.1"
                     /translation="MSGQRIRIRLRAFDHRMLDQSASEIVETTKRTGARVAGPIPMPT
                     RIERYTVNRSVHADKKSMEQFEIRTHKRLLDIIDPTAKTVDELKKLNLPAGVDIQIKI
                     "
     gene            complement(15139..17235)
                     /gene="fusA"
                     /locus_tag="PKI64_04825"
     CDS             complement(15139..17235)
                     /gene="fusA"
                     /locus_tag="PKI64_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE00370.1"
                     /translation="MAKEVKLEAAGRAHPLATIRNIGIVAHIDAGKTTTTERMLYYTG
                     RVHKMGEVHDGNTVMDWMVQEQERGITITSAATTCFWRDQQINIIDTPGHVDFTAEVE
                     RSLRVLDGAVGVLCAVGGVQPQSETVWRQANKYAVPRIAFINKMDRTGANFVRVVGEI
                     REQLNSPAVPVQIPWGQEEGFRGVVDLVRMQALTFADAELGAKVQTQAIPAELTMAAQ
                     KAREHLLEAVAETDEELLTTYLEEGDLPEAMLRAGIRRATVAGKMVPVLCGTSLRNKG
                     IQPLLDAVVDYLPSPLDVPAVKGRHPKTEAEQVRETSDFEPLCSLAFKVATDPFVGRL
                     VFVRVYSGELKKGANVYNPRTQKRERVLRLIRLHANQREEVDVLYSGEIGAIAGIKGV
                     GTGDTLCAEQKPIVLERIHFPEPVISMAIEPRTQADKEKLQEVLADLSDEDPTFQVGQ
                     NVETGQMIIKGMGELHLEILRDRMLREFKVQANAGRPMVAYRETITESARAEYTFDRE
                     LAGKHHFAMVGLLLEKNERGSGNSIDFKISSDIIPHDFRPVVEEGIRDGLSTGVLGSF
                     PLVDVRVSVVSGQWHPVDSSEIAFRSAASMSLREAALAARPVLLEPIMALEIELPEEH
                     LGDVLGDLNMRRGQVRDVAQRDGTRAIEASVPLAELFGYATTLRSLTRGRAGYTMEPT
                     RFDVVPESLCRELMNR"
     gene            17440..17793
                     /locus_tag="PKI64_04830"
     CDS             17440..17793
                     /locus_tag="PKI64_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOE00371.1"
                     /translation="MKAVVLLTISNIFMTVAWYGHLKYLPAAKWPLLGVILISWGIAF
                     FEYCFQVPANRIGYATFSAMQLKILQEVISISIFVVFAIAVLGEKLRWNHAISFLLVL
                     GAVYFAFLKPAAEGS"
     gene            17807..19468
                     /locus_tag="PKI64_04835"
     CDS             17807..19468
                     /locus_tag="PKI64_04835"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE00372.1"
                     /translation="MALWGWLLVCFLASAPLARAYEPLLLGDDERLLVLAPHPDDEVL
                     AAGGVIQEALDLDVPVQICFFTLGENNEIAALFTRRHPAFRPGPEQAFGGQRHREALA
                     AATQLGLQEEDIVFLGYPDPGTLDIWNHHWRTVPPYRSPVTRENTVPYDFALTPGSAF
                     AGEDILDDLQEVIREFQPTHVLLPHPADQNTDHRALYFFARVALWELMTEGIRPQVLT
                     CPVHLARWPEANGFQPQQPAEPPPFLAKDGQWWEYSLAPFQVSNKLAALRRYHSQFLP
                     AQAYLELFVRKTELFHELADWSFPRGQGRREVPALDTAQYRPNLALFQTLAQTAGAWR
                     EIAQQTAAEQNALANAENMLGACALDGDGANLTLTFHTQRPIEKPAELVVRLYGYRPD
                     TPFGDMPKIKLRASADGWLDVQDLDRKIAHEATVTAGPQNELILHVPYALLGNPEKLF
                     ITAKLMKGNLPIDGLPAMTLDLGDMPALALIPAAPAEPAAPPELPPAKPSKPTKPARP
                     AKPAKPAPSPSPPPAVAPPTALAPRVSLPTKAVPAHTEANEPVQW"
     gene            19462..20727
                     /gene="dinB"
                     /locus_tag="PKI64_04840"
     CDS             19462..20727
                     /gene="dinB"
                     /locus_tag="PKI64_04840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOE00373.1"
                     /translation="MVTPTGHNRPAELAPRVFLHVDMDAFYAAIEQRDHPEYRGKPLV
                     VGSPPNERGVVSTASYEARQFGIHSAMPSREAGRRCPHAIFVPPDMPRYKMVSTQVFA
                     IFGRYTPYVEGLSIDEAFLDVTSSQRLYGSGETIARRIKDDIRNELALTCSVGVAPNK
                     FLAKLGSEFKKPDGLTVLPFDRPGILAFLRPLPVTRLWGVGRVTQQAFDRAGLYTIGD
                     VQDASPALLQRVVGAHGASHLRALACGEDPREIELDIREKTISREHTFLRDEKSRATL
                     ESVLFELVEDVTRRLRADGRLATGGRLKVRWKDFDTFTRQRPFPQPTRSEQDLQTLAR
                     ALFQDIVLQQPVRLIGFGVTGLVTTTAPRQLDLFDLPIETRTKKEKLDNAVDQIRARH
                     GPAAIRRARALRQPPGPASGKNQRKEKPT"
BASE COUNT         3377 a         7133 c         6011 g         4235 t
ORIGIN      
        1 agcgacttct tgccgaagtt gcggtacttg agcatttcgt tctcggtctt ctgcgccagc
       61 tcgccgacgg tggtgatgtt ggcgttgttg aggcagttgg ccgcgcggac gctcagttcg
      121 atttcgttaa cgctgatggc cagcttgcgg gccagctctt cgcgggcggg gtccaccgcc
      181 ttttcgcttt cctggaactc ggcgatatcc ttgtcgaagt tcacgaacac gtcgaggtgg
      241 tggcgcaaga tcgcggcgga aagggtcagc gcctcatcgg ggctgatgcg gccgtcggtc
      301 cagatttcca gggtgagctt gtcgtaatcg gtgcggcgac ccacgcgggt gtcatccacg
      361 gtgtacttga cgcggcgcac gggggagaac agcgagtcaa tcggaatcag gccgatttcc
      421 tgatcgggct gtttgttcca gtcggcgggg caatagccgc ggccgatttt gacttcgact
      481 tcggcgtcaa acacgccgtc accatcgagg gtgcagatgt gctggtcggg attgagcacc
      541 tcggcggtgt tgtcgcactg gatgtcgcgc gcggtgacat cgcccgcgcc tttgaccttg
      601 atgcggaacg tgcgcggctg gcgggtgtag agcttgaaga gcaccttctt gaggttgagg
      661 atgatgtcgg tgacatcttc cacgcagccc ttcaggtggc agaattcgtg ctcggcaccc
      721 tggatgcgca cggaggtgat cgcggcgcct tcgagcgacg acaacagcac gcgacgcagg
      781 gagttgccga gcgtgcggcc atagccggcc tcaaacggct cggcgatgaa ttgggcatag
      841 gtcgaggtgg ccgtggcttc gtccttgacg acgaacttgg gcatctcgaa acggttgaga
      901 cgcgtagtca tgtagttctc cttccagtcc cgggcattaa ccggaacata tggcggtttg
      961 ggttgttact gtttacttgg aatacaattc gacgatcaac tgctcgttga cgatcggcgc
     1021 gatttcctcg cggctcggca cgtgcaggat ttcggccttg tgttcggcct tatccacgtt
     1081 gagccagacc ggccagccgc ggctttctgc tgcgtcgagg tgggccttgg cgtagtcgcg
     1141 gctgcgcttg gcctcgcggg cctggatgac gtcgccgggc ttgcacttgt aggacgggat
     1201 gttgaccttc tggccgttga cgcggatgtg gccatggcgc acgaattggc gcgcagaacg
     1261 gcgcgagggc gcgaaaccaa tgcggtagac gaggttgtcc agacgcattt cgagcgcctg
     1321 caggaggact tcgccggtga cgccgcggga gcgcagcgcg cgctggaaga acaaccggaa
     1381 ctgcccttcc tgcatgccgt acatgttgcg cagccgctgc ttttcctgca actgcaagcc
     1441 gtaatccttc aggcgcgggc ggcggccgcc gtgcatcccg gggatggagt gcccctgctc
     1501 aatcgggcac ttggccatgt agcaacggtc gcccttcaaa aatagtttta cgcccagccg
     1561 gcggcaacgc ttgcaggcgg gtccaatata tctgcccatg aactctgtcc ttcctcttac
     1621 tcttgtttag acgcggcggc gcttgcgggc gcggcagccg ttgtgcggca ccggcgtgat
     1681 atccttgatg cctgtgatcg tcaggccggc ggcctgcacg gcgcggatgg cggattcgcg
     1741 gccggagccg ggcccctgca cgcgcacttc cacttcgtgc atgccgaagc caacggccac
     1801 cttgcaggct tcctgggcga ccacggtgcc ggcgtaggcc gtgctcttgc gcgagccctt
     1861 gaagccgcaa cgtccgccgc tgctccacga aatgacgttg ccgcgcggat cggtgatgct
     1921 gacggtggtg ttgttgaacg aagcgaggat atgcaccacg cccacgggaa cgttcttggc
     1981 gcccttgacg cggcggcgct tgacttcgcc ttcggcggcg ggggtttcgg cggctggcgc
     2041 agcttcggcg gcggcaggag ccggggcgga cttcacttca tctttgattt cttcagccat
     2101 ggttcaatcc ttatttggcg gcgggggcag acttggctgc cgagcgggct tccttgccgc
     2161 ggacggcgcc gacggtcttg cgtggcccct tgcgggtgcg ggcattggtg ctggtgcgct
     2221 ggccgcgcac gggcaggccc ttgcggtggc ggatgccccg gtagcagttg acagcaatca
     2281 accggcggat gttgccggcc acgccgcggc gcagatcgcc ttccaccaca tattcttcct
     2341 gcagcatctt ggagagcgtg gcgatttctt cgcccgtcaa gtcgtcagcc ttcttggccg
     2401 gatcaatctt gaggcgcgcc accagttcct tggcgcgggt gggaccgacg ccataaatgt
     2461 attgcagcgc gtactccacg cgctttttcc cgggaatatc tacaccaatc actctgggca
     2521 tgttcgtatt tctcctgacc ttatccttgg cgctgcttat ggcgcaaatc ggtgcaaatg
     2581 acccgcacca cgcccttgcg acgcacaatc ttgcagcgct cgcaaatccg ctttaccgat
     2641 gttctgactt tcatcttcca acctcagctt tccacaatct ccacaatttc gccgcgcgac
     2701 atgtcgaacg gcgacatccg tacacgcagc cgcgcgccga ccgccgggca tagcccaccg
     2761 gtcgcgggcg catgctcccg cggaacgaac gccatacatt catgtccgtt ccctaatctg
     2821 gcacggaaaa cgcgatggtt taccacagcc acgatctccg cgtccagctc aattatttcg
     2881 cggccacgca tgtgaggatt tccgcgggcc cctgctccac caccagcacg gtgtgctcat
     2941 agtgggccga gcggcggcgg tcccgggtgc gcaccgtcca gcggttgggc agcacctcga
     3001 cctgccacgt gcccgagttg atcatcggct cgatggccag cgtcatgccc ggctgcaggc
     3061 gggggccacg ccccggttca ccgtagttgg gaatctgcgg atcctcgtgc agtttgcggc
     3121 caacgccgtg cccgcaaaaa tcgcgcacca cgctgaagcc ggcggcttcg gtcaccgcct
     3181 gcacggcgtg gccgatatcg cccacgcggt tgcccgcctg cgcggcggca atgcccgcag
     3241 ccaaggcggc ctcgcccacg cggcagagcc ggtcggattc ttcatccacc gcgccaacgc
     3301 gcactgtgat ggcgttgtcg ccgatgaaac cttcggccac aatgccgagg tccacgctga
     3361 ccaaatcgcc ggcgttgatc acccgccggc ccgggatccc gtgcaccacc tcgtcattga
     3421 gcgagatgca cgtgtgcccc ggaaatcctc catatccaaa gaacgcactt ttgacgccca
     3481 gctcccgcat ccgccgcgcg gcgaactcat cgagctcgcc cgtggtgatg ccgggaacga
     3541 cttgggccgc catctccgcc aagacttgcg cggagagtcg gcacgccacg cgcatgctcg
     3601 ccagttccgc tcgcgtcttg atgggaatca tgctgccggc gtcaagccag tttggccaat
     3661 aattccgcgg tcgccgcctg cggattggtc ccggcgttga tgcggatcaa gatgcccttc
     3721 tggtcgtaaa aatcaatcaa gctggcggtt tggcgctggt acacatccaa gcggttcagc
     3781 acggtggcct cttcgtcatc gggccgttga tacagggggc cgccgcactg gtcacagatg
     3841 ccctcttttt tcggggggat attggtgacg tgatagaccg cgccgcaggt cttgcaaatc
     3901 cgtcggccgc tcaagcggct gacgatgacc ggcgtgggca cttccaacag aaacacctga
     3961 ttcacggcgg agcccaactt ggccagcacc tcgtccagtt ggcgggcctg ctccagcgta
     4021 cgcgggaagc cgtcgaacat gtatttatcc gtcggctgcc cctgggccag ccgctcgctg
     4081 acaatgcgca tgatgacgct atcgggcacc agttcgccct tgtccatgaa cgccttggct
     4141 tccagcccgg tctggctgcc ggccttgatg gccgcgcgca gcatgtcgcc ggtggagacg
     4201 tggatgtaat ccgtggcgtt gcgcacgcct tccgccaagg ttcctttgcc cgcgccgggc
     4261 gccccaagaa gaataatggc ttgcatgcgg tgtctcctat cgttgccggt tagcggcggc
     4321 cgcgcaactg tcccttgcgc aagaagccgt catagtggcg ggtcaccagg tgcgattcca
     4381 cctggcgcat ggtgtcgagc atcacgccca cgatgatcag caggctggtg ccgccgaaga
     4441 attgggccac catccacgga atgtggaacg agttgcccag aatcatgggc agcacagcga
     4501 tgatcgtcag gaacactgcg ccggccagcg tgatgcgggt catgacccgg tccagaaatt
     4561 ccgcagtggg cttgccgggg cgaatgccgg gcacatagcc gccgtacttc ttgagatcat
     4621 ccgcgatttg caccgggtgg aactgcgtgg ccacccagaa gtaggagaag aacaggatca
     4681 tcagcccgta gagggtgatg taggtcggcc cggcatagcg caggtggacg gcggcgaagt
     4741 ttttcaccgc gtcaatcggg atcatcatca gcaaccgctg cgggaacatc aggatggcct
     4801 gcgcaaagat gatgggcatg acgcccgaat agttcacgcg caggggcatg taggtgcttt
     4861 ggccgccata ggtcttgcgg ccaaccacgc gcttggcata ttgcaccgga atcttgcgct
     4921 gggcttgcgt caccgcgatc acgccggcaa tcaccaggaa cgccagcgcc agcatggcca
     4981 ccagcatgaa gatgttggcc gaaccggtgc ggaacatggc cacagcctgg ctgatcgcgc
     5041 ccggcaggcg gctgacgatg ctgatcgtga tgatcagcga aatgccgttg ccgatgccgc
     5101 gggcggtgat ttgctcgccc agccacatca gcgtcatcgt gccggtggtc atggtcaaaa
     5161 tggccagcag caggaagggc acgcgcgaca tggtcacgat ggtttcacca ggcatgattt
     5221 gcaggccgag cgcctcgggg cgcagcagca ccgcggccat ggcgtagccc tgcaccagac
     5281 agagcgccac ggtcaggtag cggccgtatt ggatgatctg ctgccggcca gcatcgccct
     5341 cgcgcgccaa tttttccagc gcggggacca ccgccgtcaa cagttgcagg atgatcgaag
     5401 cgctgatgct gggcatgatg cccagcgcgc caatcgccgc ttgcgtcagc gcgccgccgc
     5461 tgaacaggtc cagcagcccc atgaagccca tgttgccgct ttgcgaggcc atccggtcca
     5521 tgatgccgcg gagcatctcc gtatccacgc cgggcaccgg aatcaccgac aacaggcggc
     5581 acacaaagat gagcgcgagg gtgaagaaga tgcgctggcg cagctcgggg atcttcaggc
     5641 tgttggaaaa agcggaaagc atcggggagc ctcgtggggt tcagggcgct agacaatcac
     5701 ttcacaggtg ccgccggctt gctcaatctt ggccttggcc gtggcgctaa acgcatgggc
     5761 cttgacctgg cgggccgcgg tgatttcgcc accgcccagc accttgatcc ggctcttggg
     5821 gctgacgcgc ccggcggcca cgagcgcttc cggcgtgatt tcgccgggga gcaacttggc
     5881 cagctcgccg atgttgagcg cggcatattc cacgcggttg gggctggtga agccgcgctt
     5941 gggcacgcgc cggatcagcg gcatctggcc gccttcaaac agcggcttgt gcttgtgccc
     6001 ggtgcggctc atctggccct tgttgccctt gccggccgtc cggcctttgc cggaggactc
     6061 gccccggccc acgcgcatcc ggcgcttttt cgcgccgggg gtgttcttca acgaatgcaa
     6121 attcatggtg ctctaccctt tcctgtgcct actggcgcag cgccgcggtt tcctcgcggg
     6181 aacgcagttg ctgcaggccg ttcattgtgg ccttgacgac gttgagcgcg gaacccgaac
     6241 ccagcgactt ggccagcaca tcgcgaatgc cggccaaatc cagcaccgcg cgcgcgccac
     6301 cgccggcgat cacgcccgtt cccttggtgg cgggccgcag catcacgcgc gacgcgccat
     6361 agcgcccgat cacttcatgc gggatggtcc ggtccagcat ggtcaccttg accatgttgc
     6421 gccgggcggc ttccgtggct ttgcggatgg catcggcatt ttcgcgggcc ttgcccagcg
     6481 cgtagccaac ctggccattg cggtcgccca ccaccaccaa cgcggaaaag ctgaagcgcc
     6541 gcccgccctt gaccaccttg gcgcagcgat tgacctccac cacgcgctcc agcgcttcgg
     6601 cggggctctt ctgctgaaat tcctcgttct tcttcgcttc tcgtttcatc ataaccgatt
     6661 ttccttacat taccggctct agaacttcag gccgccttcg cgggcggcat cggccagcgc
     6721 ctgcaggcgc ccgcggtagg caaagccacc gcggtcgaac acgacttcgg cgattccctt
     6781 ttccttggcg acctgcgcag ccagcgcgcc aagctgcttg gcggtggcca cattgagttt
     6841 ctggcccttg accggtccgg ccagggtcga aaccgccgcc agcgtcttgc cggcgaggtc
     6901 gtcgatgaac tgcacataca tatgcacatt ggagacaaac acgctcatgc gcggccgctc
     6961 cgcggtgccc tgcacttttt gccgcaggcg ccggtgccgg cgcacgcgat aatctttcaa
     7021 cgtcttcatt ctcatggtta cgccaccgtc ttgcccgctt tccggcgcac gcgttcgtcc
     7081 ttgtagcgca cacccttgcc cttgtagggc tcggggggcc ggaaggagcg gatgcgggcg
     7141 ctcacttgtc ccaccaattg cttgtcaacg ccgctgacct tgagctgggt gccgtcggtc
     7201 acggtcacgg tgatgccttc cggcaccgtg tattccaccg ggtgggagta gcccacgttc
     7261 agagtcaact gcttgccggc aatgctggcc ttgaagccca cgccctggat ttccagctcg
     7321 cggctatagc catccttgac gccgaccgcc atgttcgcca acagcgcgcg gatggtgccg
     7381 tgtccggccc ggctttgctt gctatcgtcc gtccggctga cgaggagctt gtcgccttcc
     7441 tggaccacgt tgatgtagtc cgggaatttc tgggtcagct cgcctttggg ccctttgagg
     7501 gtcacggcgt cgccggccac ttgggcggtc acgcccgcag gcaggaccac cggtagtttt
     7561 ccgattctcg acatagtgtt tctccctccg cccctaccag acctggcaga gcacttcgcc
     7621 accgacgcgg gcggcccgcg cctcgcggtc ggacatcacg ccgcggctgg tgctcagcac
     7681 cgccgtgccc atgccgccgc gcacgcgcgg aatctccgcc gcgcccacat agcgccgcag
     7741 ccccggacgg ctcacccgcc gcaactgctg gatgatgggc ttctcgttgt aatcatactt
     7801 cagcgtcacg cgcagcacct tcttgccgtc gcgctcggcg gtggccacat cggcgatata
     7861 gccttccttc ttcaggatcc gggcgacctc cgccttcagg cgggagtggc ccatttccgt
     7921 ggccggcaat ccggccctgc ttgcgttgcg gatgcacgtc agcatgtccg caatgggatc
     7981 tgaacagctc attctactcc tcccgtccta ccagcttgct ttcacgacgc cggggatctt
     8041 gccctcggac gccagttcgc ggaagcaaat gcggcacaat tgaaacttcc gaatataggc
     8101 ccgcgcccgg ccgcaacgcc ggcagcggct atagccccgc accgcgaact tgggttcgcg
     8161 cgaagccttt accatccatg atatcttagc caaggtattc ctccgttttc caaatgctta
     8221 cgttgcgaac ggcatgccga ggagctgcag cagctctttg gcatccgcat tgttttgcgc
     8281 ggtggtcaca atcgtgatgt ccatgccctg cacgcgcttg accttgtcgg actcgatttc
     8341 cgggaacagg gtctgctcct tgaggcccag cgtgtagttg ccacggccgt caaaggcctt
     8401 ggccggtacg ccgcggaagt cgcggatacg cggcagcgtg gcgtggatca accgctccag
     8461 gaattcatac atccgccggc cgcgcagggt gaccttggcg ccgatgggca tgccttcgcg
     8521 cagcttgaag ttcgaaatgc tcttcgtggc cttgcgctgc gtgggccgct ggccggtgat
     8581 cttggccagg tcgtccgaga cggccttgag catgtcgcga tccaccaacg cgttcaccga
     8641 catgttcacc accaccttga ccacgcgcgg cacatccatc gtggagggat acttgccggt
     8701 ggccttcagc gccttgacga catctttttg atatttttct ttcagttggg acatggtctt
     8761 attcctttcc cgtcttcttg ccgccgcggg tgaccaccac cagcttcgag gccgccatgg
     8821 gcgcctcgcg cgtgacgatc ccgccgttgg ggttgtcctg cgacttgcgc aggtgcttct
     8881 tcacaaaatt gaagccttcc accagcaccc ggccggtctt gggatacacc cgcaacacct
     8941 tgccggtctt cttgccatcc gcgtcctcgc cggaaatcgc ccggacgata tcgtcgcggc
     9001 gcacatgcat tttgggcttt ttcatcacag cacctcgggg gccaaagaga tgaccttcat
     9061 gaagttcagc tcgcgcagct cgcgggccac gggcccgaaa atgcggctgc cgcgcgggtt
     9121 tttctgttca tcaatcagga tcacggcgtt gttgtcgaag gacagcgtgg agccgtccgg
     9181 gcggtggatc ggcgcggcgg tgcgcacgat cagcgcgagg gccttgtcgc ccttcttgac
     9241 catgcccgcc ggctgcgctt cgcggatcga aacgcggatg atgtcgccaa tgccggcgac
     9301 cgtcttgcgc tggccgagca ccttgatgca cttggccgtg cgcgcgccgg tgttatccgc
     9361 caccggcaac agggattcca tctggatcat gattacacct cgctcttggc ggcgcgggtc
     9421 accacgcgca ccagccgcca ccgcttcaac ttgctcaacg ggcgggtctc ggcaatcagc
     9481 acctgatcgc cgcgccgggc ctcgttctgc tcgtcatgcg cgtaatactt tttgctgcgg
     9541 cgcaggatct tgccatacac cgggtgcgcg atgcgccgtt cggcctgcac caccaccgtc
     9601 ttgttcatcg cgtcgctcac cacgacgccc atgcgctcct tgcgcagatt gcgtgtttcc
     9661 gtcgccatat tcgtttacct cgcggcttgc gcctggttga tgaccgtttt gatgcgcgca
     9721 atgtcccggc gcaccagccg gacgcggtcg ggtttctcca actggccggc cgtctgctgg
     9781 attcgcagat tgaacagttc tttttccatc tccgccaatt tcgccaaccg ttcttcgggc
     9841 gacaggtcgc ggatatctct tgccttcatc gcgtacgctc cttacgtggt tacaggacaa
     9901 ccccgcgccg cacgaacgtc gtgcggatcg gcaactttgt cgccgccagc cgcaggcatt
     9961 ccaacgccat ggcctcggac acgccgtcca tctcgaacag gatgcggccg gggcgaatca
    10021 ccgccaccca gccctcgatg gcgcccttgc ccttgcccat gcggacttcg agcggcttct
    10081 tggaatagga cttgtcgggg aacacccgga tccagagctt gcccttgcgc ttcatggcgc
    10141 ggttcatcgc cacgcgcgcg gcttcgatct ggatgttggt cagccagcag cgctccatgg
    10201 cgcgcaggcc gtattcgccg aacgccagac tgttgccgga ttgcgccatg cccttgcgcg
    10261 agccgcgctg gctttggcgg tacttcacac gtttaggcat aaggggcata ggacttctcc
    10321 ttattcgtca cactctttgc cgcggcgtct tccggtttca cgcagatcca gcacttcacg
    10381 ccgatcttgc cggccatggt gttggcttcc gcaaagccgt actgcacgtc cgcgcgcagc
    10441 gtctgcagcg gcaccttgcc caccatgtaa tgctccgtgc gggccaattc cgcgccgccc
    10501 aagcggccgc aggcctgcac gcggatgccc agcgcgccca tgtccatggt cagttgcagc
    10561 gcgcgcttca tcgcccggcg gaacgccacg cggcgctcca actgcagcgc gatgctctcg
    10621 gccaccaact gcgcgttggt gtcgggattg cgcacttcct tgatctcgac atagatttcc
    10681 ttgccggtct tggcgctcaa ttcctcgcgc agcttgtcaa tgccctcgcc cttgcgcccg
    10741 atcaccacgc ccggccgcgc cgtgaacacc gtcacccgcg cgcgctgtgc atagcgctcg
    10801 atcacaatgt cggctacggc ggcatccttc aggcgggtgc gcaccaggtc gcggatctcc
    10861 agatcctcat tcaacagatt gccgaattct tttctttccg cgaaccagcg gctcttccac
    10921 tgcttgtgga ccgccgtacg gaacccgatc ggattgactt tttggcccaa aatgcgcctc
    10981 cttcttctcg tctacttctt cttgcgcggc ttcttgtccg tcaacgtgat tcggacatgg
    11041 gccgtctttt tgcgaatcgg cgaggctgaa ccgcgcgccc gcgcccagta ccgccgcagc
    11101 gtcgggccgt tctcaatgac cgcccgcgcc acatacagct tgtccgccga cagctccgcg
    11161 ttgttttccg cgttggcaat cgccgacttc agcgtcttgc cgatggccac cgccgccttg
    11221 cgcttgttca attcgaccac ctgcagcgct tcgcccacgc tcttgccggt aatggcccgc
    11281 gtcaggtccc gcgccttggt cggcgtcaac cgtacatact ttgttactgc tatgatttcc
    11341 atgtctcgtt cctcaaggac cttccggccg aatcagcacc gctcggtccc ctgtttgggg
    11401 gtaatcgcca cgcgcggtct cctcaaccgc cgcgccggca attctctctc tcacatccac
    11461 cacccggatc cgcgccaccc gccggcggtc ccgcagaacc gtcaagggca acccgtaacg
    11521 gacaccctgc cgggcgccct gatccaggat caccagaccc agttccgcgt tcacgtccac
    11581 cagccgcact tcgcgcggcg catccaacgg ctctaccgct ggggcgcccg ccgccgccgc
    11641 ccgccgtgct tcctgcacat ccaaggcggc ctgcgcttcg gccagggccg tgctcaactg
    11701 gcgaatccgc cgggtcagtt cggccacggc gcgccgcatc tcggcatttt cctgcgccag
    11761 ccggacggtc accaagcggg cttcgctcgt caccaggtcc aatacttcat catgatggcc
    11821 atccgccgcg ccgcccatgg ccgctgctgc cggcccgtcc gtgcggatgg gaatgttcgc
    11881 ttcatcctgc gcggactgca ccgaaagctc ggccgtctcc gcctcaaccg gagcctccgg
    11941 gactggcccg ggctccatct caagacgcgc cggcgcggac gccggccctg tcgtcaaaga
    12001 actattttgc gcttgccgcg tgccgaacca ccagcccgcc gccagcgcgg ctcccaccac
    12061 cggcacggcc cagatcagcc gccagcctcg acggaagccc atcatccaaa aatctcttac
    12121 ttcagcgcga tcgtcttatc cgtcgccgcg ccgtgcttgc ggaacgtccg ggtgggcgca
    12181 aactcgccca ggcggtgtcc caccatgttt tccgtcacga acacgctcgt gaacaccttg
    12241 ccgttgtgca ccgtgaacgt atggcccacg aattccggca ggatcatgct gcggcgcgac
    12301 caggtcttga tcggacgctt ggcgccactg gcgttcagcg cttccacctt cttcagcagg
    12361 tggtcttcca catacggtcc ctttttgatt gagcgcgtca tgttacttct ttctccgttc
    12421 cacgataaac ttgctcgacg gcttgcggcc gctgcgggtc ttcttgccct tggtcaactg
    12481 gccccacggg gtcaccgggt gcccgccgcc ggaggtccgg ccttcgccac cgcccatcgg
    12541 gtggtccacc gggttcatcg ccacgccgcg caccgtcggg cggatgccct tccagcgctt
    12601 gcgcccggcc ttgccgaggg aaatgttttc gtgctcgagg ttgcccacct ggccgatggt
    12661 ggcatagcat tcgccgcgca ccttgcgcag ctcgccggag ggcagcttga cctgcacata
    12721 acccgcgtcg cgcgccgtca cccgggccac catgcccgcg ctacgcacca tcttggcgcc
    12781 ggcgccgggg gtcagctcca gggcgtggat cggcattccg atcggaatgc tcgccagcgg
    12841 cagcgcattg ccgtccgttg gttccgcttc cggcccggcc atgacggtgg cgcccacttg
    12901 cagcccgttc ggcgccagga tgtagcgctt ttcaccgtcg gcataggcca gcaacgccaa
    12961 ccgcgccgtg cggttcgggt catattccaa cgcctgcacc ttcgcgggaa tgtcccgctt
    13021 gtcgtgccgc atgaaatcca cctgccgcaa aaacttttta tgcccgccgc cgcggtgccg
    13081 caccgtgatt cggcctgccg cgttgcgccc ggccttgctc ttcttgattt tgatcagccg
    13141 gcgttcgggg cgcttcttgc tcaggccttc gaaatccgaa agcgtggtga agcgcaatgc
    13201 cgccgtggct ggcttgtatt ttttcaaacc catgttcgat cctcgcttat gccatctcga
    13261 tggtgtgtcc ggcctgcagc gtcaccaccg cgcgcttcca gtccggggtc cggccatacg
    13321 ccgccgtccg cagccgcttg gccttgcccg ggcggaccat cgtattcaca tccttgacct
    13381 tcacgttgaa ccgctgttcc acctcgcggc ggatttccag cttgttcgcg gcgcgggcca
    13441 ccgcaaaaat gtattggttg ccggtttctt tcaggcgcgt gcccttttcc gtcaccagca
    13501 gctttttgac aatcaggttc ttcatggttt acttcgcccc ttctgccagc cgcgcctgca
    13561 gcgccgtcaa gccggccgcg tccgtcacga tgaccggata acgcatgatc tggtacgtgt
    13621 tggcctgcgc cgccgtggtc acttccaccc gcggcatgtt gcgtgccgcg cgcacgagat
    13681 tctgattcgg cgcaccggtc acgaacagcg tggcttgctt ccccgccgcc ttctgcagca
    13741 ggcccgccag cgccttggtc ttgccttcca cgctctccag cccgttggcc accaccacgc
    13801 cgccggcctt gatcttctcg ctcagggctc gccggaacgc cagcgccaca accttcttgt
    13861 tgactttctt gtcgtagctg cgcggatgcg gaccgaacac cacgccgccg cctttcagca
    13921 gcggggagcg gatggacccc tgccgcgcgc gcccggatcc cttttgcttg aaaggcttac
    13981 ggccgccgcc gctgacttcg ccgcgcttct tggtggaagc cgtgcccttg cggaggcccg
    14041 cccggtacgc cgtcaccaca tccagcaccg cttggccgcc gcgcttggtt tccagcaggt
    14101 ccgccgcaaa ctcctgttcg ccggcggcct cgccgttcaa attgtagatg gcaattttgc
    14161 tcatgactcg tcctacgcct tcttgatcgc cttgcgcacc agcacaatgc cgcccaccgg
    14221 gcccggcacc gcgccttcca gcagcagcgc gtggtcgtcg gccaacacct tgaccacccg
    14281 caggttctgc gtggtgacgt tcaccgagcc catgtggccc ggcagcttct tgttgcggaa
    14341 aatccggccc ggctcttcac gcccgccgat ggaccccggc cggcgcatcc agccgccgcc
    14401 gtggctcgcc cggccaccgc cgagcttgaa gcgcttcaca acgccctgga acccgcggcc
    14461 tttggtgtgg cccagcacgt ccacaaactt cacgccttca aaaatcttcg cggtcagcac
    14521 atcaccggcc ttggcttcct cacccgccgc caggcgcact tcgcgcagca cttttgccgg
    14581 cttggcgccc gccttcttga aatgcccctg ttgcggcttg gtcaggcgcg attccttctg
    14641 ctcttcaaag cccaattgca ccgcttcgta gccgtccggc tgcgcgctct tgcgctgcac
    14701 caccgtgcac ggacccacct gcaccaccgt caccggcacc tgcacgcccg cgtcatcata
    14761 cacgcgcgtc atgccgatct ttctgccaat caatgcttgc atggctgtcc tccccctcaa
    14821 atcttgatct ggatgtccac gcccgccggc aggttcagct tcttcagctc atccaccgtc
    14881 ttcgctgtgg ggtcaataat gtccagcaac cgcttgtggg tgcggatctc aaattgttcc
    14941 atggacttct tgtccgcgtg gacgctgcga ttcaccgtat agcgctcgat gcgcgtgggc
    15001 atcgggatcg ggcccgccac gcgggcgccc gtgcgcttgg tcgtctcgac aatttcgctg
    15061 gccgattggt ccagcatccg gtggtcgaaa gccctcagcc gtattctgat tcgttgtccg
    15121 ctcatgttct ctattctttc aacggttcat caactctcgg cacaggcttt ccggcaccac
    15181 atcgaaccgg gtgggttcca tggtgtaccc ggcgcgtcca cgtgtcaaag aacgcaatgt
    15241 cgtggcgtat ccaaacaact ccgccaaggg cacggaggcc tcgattgccc gcgttccatc
    15301 ccgctgcgcc acgtctctca cctggccgcg gcgcatgttc aggtcgccca gcacgtcgcc
    15361 gaggtgttcc tcgggcaact caatctcgag cgccatgatg ggctccagaa ggaccgggcg
    15421 cgctgccagc gccgcctcac gcagcgacat tgaagccgcc gaccgaaagg cgatttccga
    15481 cgagtcaacc ggatgccatt ggccactaac caccgacacc cgcacgtcaa ccagcgggaa
    15541 acttcctagc acgcctgtgc tcaacccatc gcggattcct tcctccacaa caggtcgaaa
    15601 atcgtgcggg attatgtcgg aagaaatttt gaagtcaata ctatttccgc ttccccgttc
    15661 atttttttcc agcagcagtc ccaccatcgc aaaatggtgc ttcccggcca gttcccggtc
    15721 aaatgtgtat tccgcccgcg ccgactctgt aatcgtttcg cgatacgcca ccatcggccg
    15781 ccccgcgttg gcctgtacct tgaactcccg cagcatccgg tcgcgcaaaa tttccaggtg
    15841 cagttcgccc atgcccttga tgatcatctg ccccgtttcg acattctgcc ccacttggaa
    15901 tgtcgggtcc tcgtctgaca gatccgccag cacctcttgc agtttttcct tgtccgcctg
    15961 cgtgcgcggt tcaatcgcca tcgaaatcac tggctccggg aaatgaatgc gctccagcac
    16021 aatcggtttt tgctcggcgc acagcgtgtc gcccgtgccc acacccttga tgcccgcaat
    16081 cgcgccgatt tcgcccgaat agagaacatc cacttcttcg cgctggttcg cgtgcagccg
    16141 gatcagccgc agcacccgct cgcgcttttg cgtgcgcggg ttgtacacgt tcgcgccttt
    16201 tttcagctcg cccgaataca cacgcacaaa caccagccgc cccacaaacg ggtccgtcgc
    16261 cactttgaac gccagcgaac acaacggctc gaagtcgctg gtctcgcgca cctgttccgc
    16321 ttcggttttc ggatgccgtc ccttcaccgc cggcacatcc aatggcgacg gcaaataatc
    16381 caccaccgcg tccagcagcg gctgaatccc cttgttgcgc aaactggtcc cgcatagcac
    16441 cggcaccatc ttgccggcca ccgtcgcgcg gcgaatcccc gcccgcagca tcgcttccgg
    16501 cagatcgcct tcctctagat aagtcgtcag cagttcttca tccgtctctg ccaccgcctc
    16561 cagcagatgc tcgcgcgcct tttgcgccgc catcgtcaac tctgcgggga tcgcctgcgt
    16621 ttgcactttg gcccccagct ctgcatcggc aaacgtcagc gcctgcattc gcaccaaatc
    16681 caccaccccg cggaatcctt cttcctggcc ccacggaatc tgtaccggca ccgccggcga
    16741 attcaattgc tcacgaatct cccccaccac ccggacaaaa ttcgcccccg tgcggtccat
    16801 tttattgatg aaagcaattc gcggtaccgc atacttgttc gcctgccgcc acactgtctc
    16861 cgactgcggc tgcaccccgc ccaccgcgca cagcaccccc accgccccgt ccagcacccg
    16921 cagcgaccgc tccacttccg ccgtaaaatc cacgtgcccc ggggtgtcaa taatgttgat
    16981 ttgctggtcc cgccaaaaac aagtcgtcgc cgcgctggtg atggtgattc cccgctcttg
    17041 ctcctgtacc atccagtcca tgaccgtatt gccgtcatgc acctcgccca tcttgtgcac
    17101 ccgccccgtg taatacagca tccgttccgt cgtggtcgtt ttccccgcgt caatatgcgc
    17161 cacgatcccg atattccgga tcgtcgccag cggatgcgcc cggcccgccg cttccaattt
    17221 gacctctttc gccacgttcc tgtgtttcct tatatatgaa taaagggccg caacctacca
    17281 tgcccccccg cccgccgcaa gaccgaaccc cctgctccaa ttcgcgccag cggtacgctc
    17341 actgcggggc gtcgccgccc gcccccgcgg tgttttgccc gcggttttga ctttgcaacg
    17401 cgggggcgct ctttctatga tggcgcggaa tacgagctta tgaaagcggt tgttttactg
    17461 accatttcca atatattcat gactgtggct tggtacgggc acttgaagta cctgccggcc
    17521 gccaagtggc ccctgctagg ggtcattttg attagctggg gcatcgcgtt tttcgaatac
    17581 tgcttccaag tgccggccaa ccgcatcggc tacgccacct tttccgccat gcagctcaaa
    17641 atcctgcaag aagtcatttc gatcagcatc ttcgtggtgt ttgcgattgc cgtgctcggc
    17701 gaaaaactgc gctggaatca cgccatctca ttcctgctgg tgttgggcgc ggtgtatttc
    17761 gcttttctaa aacctgccgc ggagggctcc tgacatgtgc gggcgaatgg cattgtgggg
    17821 ctggctgctg gtttgcttct tggcctccgc gccgttggcg cgtgcgtacg aaccgctgct
    17881 gctgggcgat gatgagcgct tgctggtgtt ggctccgcat ccggacgatg aagtcctcgc
    17941 cgccggtggc gtcatccaag aagcgctcga tctcgatgtg cccgtgcaga tttgtttttt
    18001 cacgctgggc gagaataacg agatcgccgc gctgtttacc cggcgacatc ccgcgttccg
    18061 gccggggccg gagcaggcgt tcggcggcca acgccatcgc gaagccctgg ccgcggccac
    18121 ccaattgggc ctgcaggagg aagacattgt cttcctgggc tacccggatc ccggcacgct
    18181 cgacatctgg aatcaccatt ggcgcaccgt gccgccatac cgctcgccgg tgacgcgcga
    18241 aaacaccgtg ccctacgatt tcgccctgac gcccggctcc gctttcgccg gcgaagatat
    18301 tctggacgat ttgcaagagg tgatccgcga gtttcaaccc acccatgtcc tgttgccaca
    18361 cccggccgac caaaacaccg accaccgcgc gctctacttt ttcgcgcggg ttgcattgtg
    18421 ggaattgatg acggagggca tccgcccgca agtcctcacg tgccccgtgc atttggcgcg
    18481 ctggccggaa gccaacggtt ttcaaccgca acagccggcg gagccccccc ccttcctcgc
    18541 caaggacggg cagtggtggg agtattcgct cgcgccgttc caggtgtcca acaagttggc
    18601 cgcgctgcgg cgctaccaca gccaatttct gccggcgcaa gcctatttgg aattgttcgt
    18661 gcgcaagacg gagctcttcc acgagctagc ggattggtcc ttcccgcgcg gccaaggccg
    18721 ccgcgaggtg ccggcgctgg ataccgcaca gtatcggccc aatctggcgc ttttccaaac
    18781 gctcgcccaa accgccggcg cgtggcgcga aatcgcgcag caaaccgccg cggaacaaaa
    18841 tgcgctggcc aacgcggaaa acatgttggg cgcttgcgcg ctggatggcg atggcgccaa
    18901 tctcacgctg acgttccaca cccagcgccc aatcgaaaag cccgccgagc tggttgtgcg
    18961 gctttatggc taccgccccg acactccgtt tggcgacatg ccgaagatca agctccgggc
    19021 cagcgcggat ggctggctgg atgttcagga tttggaccgc aaaatcgcac acgaagccac
    19081 cgtcacggcc ggcccgcaaa acgagctcat tttgcacgtg ccgtacgcgc tgctgggcaa
    19141 tccggaaaaa ttgttcatca ccgccaagct catgaagggg aatctgccga ttgacggtct
    19201 gcctgccatg acgctggatt tgggcgatat gccggccctc gcgctgatac cagccgcacc
    19261 ggcagaaccc gctgctccgc cagaacttcc ccccgccaaa ccaagcaagc ccacaaaacc
    19321 cgccagacct gccaaaccag ccaagcctgc tccgtctcct tctcccccgc ctgccgttgc
    19381 gccgccgact gcccttgcgc cacgcgtgag cttgccgacc aaagccgtcc cggcccatac
    19441 cgaggccaac gagccggtgc agtggtgacc cccaccggcc acaaccgccc cgcggaattg
    19501 gccccgcgcg tgtttctaca tgtggacatg gatgcttttt atgccgcgat cgaacaacgc
    19561 gatcatccag aatatcgcgg caaaccgctg gtggtcggct cgccgccgaa cgagcgcggc
    19621 gtggtctcca ccgcatccta tgaggcgcgt caattcggca ttcattccgc catgccttcg
    19681 cgtgaggccg gccgccgctg cccgcacgcc atcttcgtgc cgccggacat gccgcgctac
    19741 aaaatggtgt ccacccaagt cttcgccatc ttcgggcgct acacgcccta tgtggaaggg
    19801 ctttccattg atgaggcgtt tttggatgtc accagctcgc agcgcctcta cggttccggc
    19861 gaaaccatcg ctcggcgaat caaggacgac atccgcaacg agctcgcgct gacctgttcc
    19921 gtcggcgtgg cgcccaacaa gtttctggcc aagctcggct cggagttcaa gaagcccgat
    19981 ggcctcaccg tgctgccgtt cgaccggccc ggcattctgg cttttttgcg gccgctgccg
    20041 gtcacgcggc tctggggcgt gggccgcgtg acgcaacagg cgtttgaccg cgccgggttg
    20101 tacaccatcg gcgatgtgca ggatgcttcg cccgcattac tccaacgcgt ggttggcgcg
    20161 cacggcgcaa gccacctgcg cgcgctggcc tgcggcgaag acccgcgcga aatcgagctg
    20221 gatatccgcg aaaaaaccat ttcccgcgag cacacatttt tgcgcgacga aaaatcccgc
    20281 gctacgctcg aaagtgtgct gttcgagctg gtggaggacg tgacccggcg actgcgggcc
    20341 gatggccggc tggccaccgg cgggcggctc aaagtgcgct ggaaggattt cgacaccttc
    20401 acccgccaac gcccattccc ccagcccacg cgcagcgaac aggatttgca gacgcttgcg
    20461 cgcgcgctgt ttcaggatat tgttctccaa cagcccgtgc gcctgatcgg cttcggcgtg
    20521 accggcttgg tgacgaccac ggccccccgc caactggatt tatttgattt gcccattgaa
    20581 actcgcacca aaaaggagaa actggacaac gccgtagatc aaatccgcgc gcgtcacggg
    20641 ccggcggcca tccgccgggc ccgcgcattg cgccagcctc ccggcccggc ctccggcaaa
    20701 aaccagcgta aggagaaacc aacatgaccg cccccgcccc ccgccccccc cccccc
//
LOCUS       DAOPNP010000036        20363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3244_length_20363_cov_9.271026, whole genome shotgun sequence.
ACCESSION   DAOPNP010000036 DAOPNP010000000
VERSION     DAOPNP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3244_length_20363_cov_9.271026"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..806
                     /locus_tag="PKI64_04845"
     CDS             450..806
                     /locus_tag="PKI64_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOE00374.1"
                     /translation="MSENTSYIAQWMGQMRRGWVETCVFNVLANQELHGYGIMLRLQD
                     IPGLGITEGTLYPLLSRLRLQGLVTVRRWEESPDGPARKIYGLTARGRQILELMNDHF
                     EVMRRQHQAARKGTRP"
     gene            803..3412
                     /locus_tag="PKI64_04850"
     CDS             803..3412
                     /locus_tag="PKI64_04850"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOE00375.1"
                     /translation="MIQWNPDAQRVFDLHCEEQRAVLLAAGADPDEVFGDWRALVETR
                     AAAAGETEVQVARVQTELAKLAEALGAFAASRQEASAPLAASARPAWRVWFRRGVTLL
                     LWLLGVALPLGVLVFELIAGFCAELLFDPMPTWLHTLLIALVPAANAWALWAAAGRCQ
                     PRPTLRILNGMAVGIAAFYALQFAIITPFAAVAILYFGIGLVPLAPLLSLWAAIVLRF
                     RLLRLPAESRTQFPGLWRTALPAFLLLLVLTVPRLAIIRYTPDIESPDRAVQIRAVRI
                     LRAVGDRDQLLRQCYRPLETFDLFAMLVGHPFWLGGANGISRDTAQAAYYRVTGTPYN
                     AVPPPRVKGFRGQELLNADWFDPALGSDRIAARIKNLALAQSRLDGRVDAASGIAYLE
                     WTLEFRNTSPVQREARALIELPPGGVVSRVTLWINDEPREAAFGGRSQTRQAYQEVAV
                     QQRRDPVLVTTAGPDRVLVQCFPVPVDGTIKTRIGITAPLTVLMATGAEAMLRLPALV
                     EQNFGVAANVQTTVWLESDVPASASAGLSSSVGEDNVTLRGKLAADSSAGAAHLALPM
                     AIPYPPRVAKDERLPDRQAILQTLKLPETTPSLPAALALVVDGSALMKSRAATIQALL
                     AAIPPETRLYCVVAGDDAIATEGLPGARSLRFVGGCDNGPALALAAEWAATHDYAPIL
                     WLHAAQPLQSTELEALRQAVDFSRGKLVIHSVQFGPGVDLITEKMADLRVVRPLPVVE
                     ATATDILDALRDPGATWQRALVADADVPADASEGSSHIVRLWAADEIGQLSASYRKGG
                     TTDAVKMARTFQLVTPVSGAVVLETAAQYAAHDLTPADDTTTPGIVPEPGTLGLLLLG
                     GSALWAMRRRRRR"
     gene            complement(3432..4232)
                     /locus_tag="PKI64_04855"
     CDS             complement(3432..4232)
                     /locus_tag="PKI64_04855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE00376.1"
                     /translation="MSAWATSLSAVLMGLVEGITEYLPISSTGHLILVERCLRVSFHE
                     SFDVVIQSGAVLAVLVEYRRRFVRLAKLRQADGFAGWRGLWWLLLTTLPAMVLGLLAH
                     GTIKEYLFTPGVIAGALMVGGVAMIALERRLPELQDEGVDALGWQRALGIGLFQCLAM
                     IPGTSRSMATILGGRLLKLSRRAAAEYSFFAAVPVLLLAGAYDFYKGLPTLQRADWLP
                     YIIGITVSFLVARLAIRWLMRLISSHTLEGFGWYRIAFGVVIGVALLR"
     gene            complement(4248..5825)
                     /locus_tag="PKI64_04860"
     CDS             complement(4248..5825)
                     /locus_tag="PKI64_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE00377.1"
                     /translation="MKKVLIPTALDAVAKTMLEAHGGYAVVQVPKADLAQLVRDNPDV
                     HALIVRSEKITADIIDAAPNLKVIVRAGAGYNTIDIRHARKKGVDVMNTPGANSNAVA
                     EEVVAMMLADARHLVAADASVRAGEWEKTAFMGTELTGKIVGIVGLGYIGQLVAKRLS
                     GFEMKMIGYDPFTSAEKAAALGIELTSLPELFAQADYVTLHIPENEETRGMVNRALLS
                     RMKPGATLINCARAGVINEADLREVKATAKIRFLNDVYAKDESGQKSVADIADLMLPH
                     LGASTREANRKAAEMAAQELIDLDDRGITSAVVNRDVPAGLDPAFGRLANVLARICRR
                     VLGPDRQLKLVETSFYGSLKPYAKWLLTPVTTGLSADYEPTLDHKATLSFLADMGVDY
                     VDRVTDDHKAYTNSITIDLTGNIDAERLARASVRGTVEEGNLLVSRINDFDKLYVEPA
                     GNWVVFIYEDRPGVLGQIGAALAAAGVNIDDVRNPHDSKGVNSLAIIKVNQPVSDEIV
                     AQIAADIQARTAFHIEL"
     gene            5933..6934
                     /locus_tag="PKI64_04865"
     CDS             5933..6934
                     /locus_tag="PKI64_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE00378.1"
                     /translation="MLQPAFIRRGLGWLGLLLVWGAAMLPHPAQAAAKPYVEKAPKRG
                     FHLFLHAKQKTPTAQWDYVQDLARAGKAKAAAKQALALRIYWPNAPEAPQAQMFYARH
                     LDRRQNIQDAFDAYQHLVDHYPGRFEFDELISRQMQLAKTLMTLKKGHFLFIPGFAAP
                     ERAIPLFEKIVATAPESPLAAEAFYCLGVANERTYEYEKAIDAYFTVLNRFPESAFAE
                     DAAYSQAQCHIKLAQESPNDNRALETASAACTLFLQRFPDSARRTEIIASQARLQRLQ
                     ADNAYARARYYDRILHKPEAALIAYRHFLALYPQAEQAAEAKQRVAQLTPATPPPEN"
     gene            6936..7445
                     /locus_tag="PKI64_04870"
     CDS             6936..7445
                     /locus_tag="PKI64_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOE00379.1"
                     /translation="MKRIALILVAAMICLTGGCVQYRLGSMLPADIRTVYIPTCDNQT
                     SEPLIEQDVTQSILSQIQMDGSLRVTTEDLADSVLRITLTKFWLEPVAYEAGESSTAN
                     QYRMSIRASFVLQRKATGAVVVEAPAVTGWYDFDFMGDMSSSKSVALRPAANDLGRRI
                     VNQIVQYWP"
     gene            7457..7867
                     /locus_tag="PKI64_04875"
     CDS             7457..7867
                     /locus_tag="PKI64_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOE00380.1"
                     /translation="MKELEEGTALTLDFNKLTKAVSACPGVIPVAVQNADTHEVILVA
                     YTNEIALKKAIAERTAIFWSTSRNELWEKGKTSGETFELLEVRVNCEQNSLLYLVRPR
                     RGGICHTKNQTGQPRNCYYRRLDFQTGQLVNLDP"
     gene            7874..8881
                     /locus_tag="PKI64_04880"
     CDS             7874..8881
                     /locus_tag="PKI64_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE00381.1"
                     /translation="MTSAATPPPPPAHDPARAAANRRILAFVVLFLLLLGGSVWLVLH
                     QEKVRGRLIQPEQPAAPETEVPAPPKPPPAPPPPVTKPVSPALAQSEQMEAALTAYRE
                     AGEFLVAKQFGPAEDRALAALKAYPKLAGAQRILGLIYIQQGNFDRAISVLEAALRNE
                     PFNPEALTNLAFAYLQTRNTDLALELIETCRRLHPDYQPALLQEGIMLLSLPPSERAV
                     TVLREAVAAFPNMPGPRNNLAIALARMGDREAAREQLQVVLEMEADNVSALFNMGAFY
                     AQETNAPAAVPWLRRAMQHVAPGSFRDFLNDPDLANIRGTPEFLQLLQELDPAVPGPR
                     P"
     gene            complement(8878..9615)
                     /gene="hisA"
                     /locus_tag="PKI64_04885"
     CDS             complement(8878..9615)
                     /gene="hisA"
                     /locus_tag="PKI64_04885"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOE00382.1"
                     /translation="MSDFVILPAIDLKDGQCVRLRQGRADDVKVYGHDPAAQAADWAR
                     QGAQWLHVVDLDGAFQGRPVHGAVIAAMVQAAGIPVECGGGLRRDEDIETLLACGVRR
                     AILGTRALEDMAALAQLAAKYGDRLAVGIDARNGLVQVKGWTETSRMTAVELARRAAA
                     SGIKTIIYTDTATDGMLQGPNISGVAEMCDVVGPGCMVIASGGITTAADIRRLRAQNK
                     ANLGGAIVGKALYEGRTTLAELQAAAS"
     gene            complement(9661..10251)
                     /gene="hisB"
                     /locus_tag="PKI64_04890"
     CDS             complement(9661..10251)
                     /gene="hisB"
                     /locus_tag="PKI64_04890"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOE00383.1"
                     /translation="MKTRKAIIERKTRETQIRVELNVDGHGTYQIETGLPFFNHMLEL
                     FAKHSLLDLNLRAKGDLEVDHHHTVEDVGLALGDALNQALGERKGIERYGFSLLPMDE
                     TLSRVALDLGGRPYLVLQMANKKKKILDFELSLLGEFLRAFVTQARMNLHIHQLYGAD
                     AHHAWESVFKGLARALKMACRHDPRVKGVPSSKGIL"
     gene            complement(10319..11551)
                     /gene="tyrS"
                     /locus_tag="PKI64_04895"
     CDS             complement(10319..11551)
                     /gene="tyrS"
                     /locus_tag="PKI64_04895"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE00384.1"
                     /translation="MWKLSDAMLTLDQQFAKITARRVDLHSAEELKNRLAKAQAENRP
                     LRVKLGADPSAPDLHLGHCVALNKLREFQDCGHTVVFIIGDFTGMIGDPSGKSATRPQ
                     LSKEQVMANAKSYQEQVFKILDPARTEVRFNSEWFGPMVFEKVIRLSAHVTVAQLLAR
                     DDFAKRYAANQPISLVEFLYPLVQAYDSVMVRADVELGGTDQLFNLLLGREIQKAHGQ
                     EPQIVMTMPLLEGLDGVNKMSKSLGNYIAVNDTPKDIFGKTMSVSDDLMWRYFSLVLC
                     LPEDEIAALRAAVANGTRHPREVKDELARRIVAKFHGDAAGNEASAEFARVFAQHELP
                     TDIPEVVLPAGKIGILHLMVKAGLAPSTSEARRLVQAGAVKLGDDKVSDFRLEIEPAD
                     GLVIRSGKRGFAKIRRQP"
     gene            complement(11535..12551)
                     /locus_tag="PKI64_04900"
     CDS             complement(11535..12551)
                     /locus_tag="PKI64_04900"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOE00385.1"
                     /translation="MKKIDWNSLGFAYMDTNCHIRYVWKNGQWSAGELVASPQMSIHI
                     AATALHYGQSAFEGLKAFRRKDGKVSLFRPEENAKRLAMTARRTCMAEVPVDMFVDAC
                     KRVVRANLDYVPPYGTGGSMYVRPLLFGSGAQIGVAAADEYTFLVLVTPVGPYYKGGL
                     QAVKAIVLDQYDRAAPLGTGHVKVAGNYAVSLYPHLEAKAAGYPVELYLDAKEHKWIE
                     EFATSNFLGITKDGKYVTAKSGSILPSITNLTLQQIARDNGIPVEVRPIAFEEVTEFA
                     EVAACGTAVVVTPVSEIERAGTVYKTGPATGCGPVLEKLYKTVQGIQYGELPDTHGWN
                     VEVE"
     gene            complement(12737..13726)
                     /gene="pta"
                     /locus_tag="PKI64_04905"
                     /pseudo
     CDS             complement(12737..13726)
                     /gene="pta"
                     /locus_tag="PKI64_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
     gene            complement(13786..14940)
                     /locus_tag="PKI64_04910"
     CDS             complement(13786..14940)
                     /locus_tag="PKI64_04910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00386.1"
                     /translation="MKVSFLVPDLGWPIVGIAARMANYLHPEHEVEIVGPNLWRGANI
                     MYAAEFAYRPVDCPRLYRLPEYFRESRKLTRALTGDVIISMKAFGSNLPAALRAKRER
                     GCRVVAYLDEWDGAVAAEWSWSERLRQWTRDWMHPGNNVYVPWVERRLRECDERLVTT
                     QFLAQKFAGRIFHIGVDTARFQPQDPEAVARLKHQLGLSANTLLVFGGVARPHKGLEV
                     FLEALVRLRRTDCRVVILGPHNEYVQQLQQHSEYGALVCCPATTPEAIQLIHREMPLY
                     LGLGDALLVPLTDTLLARSQMPCKLFEAMAMGKPILANAVSDVPAVLGDGGYLLPPND
                     VAATAQAIQALLADPAGAAAMGQRARARCVAHYSAATSRQELLNILQALR"
     gene            complement(14937..16214)
                     /locus_tag="PKI64_04915"
     CDS             complement(14937..16214)
                     /locus_tag="PKI64_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE00387.1"
                     /translation="MTKRNVIVLGAGLAGLGFARHYPGCRVFEADDTPGGHARSHSFA
                     GVWFDRGAHICHSSDPEWLALLHAAAPLHVMTKSTVLNHKQGKWFAYPVQNHLFDLPP
                     PEREAALRDFLAAQEKYRGREPANYEEWCRFQYGDYLLENFYRLYTAKYWHVPMAELA
                     TDWLGGRLLPSQVENIIAGANGPVAEQQAVFRTFRYPQSGGFFAMLARLADGVDVRLQ
                     KRAVKVAAREGRVSFADGTAESFDWLVATIPLPELVAMMPEAPSAVREAAAKLRALRQ
                     YCVNLVVNRPALSPAQWFYIYDPDVAVSRVSFPFALSGQASNQTAIQAEIFLPQNARP
                     NEAQLRDQAIADLGRILRFAPDEVTAAEIRCYPLSYVVSDLARAPAVRLMRDWLRTQR
                     IVTAGLFGEWHYVWSDRAYASGRNLAQTLLAAP"
     gene            complement(16288..17184)
                     /locus_tag="PKI64_04920"
     CDS             complement(16288..17184)
                     /locus_tag="PKI64_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE00388.1"
                     /translation="MSASPPIVVTGAAGFIGRRTVARLAQAGCRVWALDRVPWPGEPL
                     AGVEHYCVDLSTDAPPVTEGTLVHLAWNMARADAAGQTQALADFQRLLSAGSWRGVVG
                     LGSAEEYGDLAGRLREDQAPGPLLSAYGQAKFAAGQALAAWARAANRKAVWLRPFVVY
                     GPGQGGSMAIPFALRCARERIPGDFSAGHQLRDFVHVDDVVAGIVAATLGLPALKTSW
                     TVCNLGRGEPVRLRDVLERIGELTQATELFRFGVRPMRPNEPPEQVADVATAAAVLGW
                     RAQISWQRGIAELCKATMEPRG"
     gene            complement(17234..18016)
                     /locus_tag="PKI64_04925"
     CDS             complement(17234..18016)
                     /locus_tag="PKI64_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOE00389.1"
                     /translation="MQNKDIPVMILAGGRGTRLMEETRDIPKPMVTIGGKPILLHLMQ
                     YYSRFGFRKFIICLGYKGDVIKEYFLHVRERTATVRLHAGGRVEYESGIATDWDIILA
                     ETGEHSLTATRILRAADYVDAPLFALTYGDGLSDIDLNAELAFHRAHGKIGTMAAVHP
                     PSRFGMMELAPDGKVRVFREKEKLHNDYINGGFFLLQKEFLARLPSQENVSLEAAPLT
                     DLAAAGELYAYRHEGFWGCMDTMRDRENLEKIYESGSAPWAG"
     gene            complement(18029..19102)
                     /gene="rfbG"
                     /locus_tag="PKI64_04930"
     CDS             complement(18029..19102)
                     /gene="rfbG"
                     /locus_tag="PKI64_04930"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOE00390.1"
                     /translation="MTFGETYRGKRVWLTGDTGFKGSWLAFWLHRLGAEVFGIGLEPD
                     TAEGPFVRAELPHLIRHETADIRDAARMLTLVRDFQPEVVFHLAAQALVRRSYVEPVE
                     TFDTNVRGTAHVLEAIRQAGQPCAVVVVTSDKCYENREEDYAYREEDPMGGHDPYSAS
                     KGCAELVAASYRRAFFAAPGRVHLATARAGNVIGPGDWAADRIVPDAVRAIRAGKALS
                     VRNPRAVRPWQHVLEPLSGYLRLGQALLEQGARAAEGWNFGPDPASARNVGELAELFC
                     RAWGDGATWQAMPNPANPHEAHLLQLAIDKARERLAWHPKWNFETTVQRTAAGYRTML
                     TARNVEAVRAFMNDEIAAYEAAT"
     gene            complement(19102..20043)
                     /locus_tag="PKI64_04935"
     CDS             complement(19102..20043)
                     /locus_tag="PKI64_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00391.1"
                     /translation="MKSRRKVLMILTHDRLDCLRLCLEMLEKANAFAAFDHVVLLLNG
                     VPARLRRYVQQFMAARPQVRWDTVDGDGTRPGGICAVQNECIRRYPDSIYVKVDEDVF
                     VPTGWAERMLATYERYQDRTDLALITTLIPNNAYGLHRLLTTFYPEKLPEFERLFGAP
                     PSAAAKGPTWQRPDIAEWATRLFLDLEAANEEQRRRLAASDQPRWHEFCQYFSIGCIC
                     YDYAHVQRMGGALPPRDEPDWCAWFEEKKHICVLDQSMVVLHYSFFVQQDWLDRSTLL
                     EDIRARNLPGTSPWYAAGARWWRLARQVPRILKRRLA"
     gene            complement(20261..>20363)
                     /gene="rrf"
                     /locus_tag="PKI64_04940"
     rRNA            complement(20261..>20363)
                     /gene="rrf"
                     /locus_tag="PKI64_04940"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3976 a         6614 c         5924 g         3849 t
ORIGIN      
        1 taccaagaat ttagcaggtc aatgcttcat aacctgcttc tatgggatga tagtgtccag
       61 agtttataaa ctctgggcag atgccgaaat gttttcatct cactcatctg caatttatta
      121 catttatttt ttagtctatt tttaatcggt cattttacga gcactgtcca cactttataa
      181 actctggaca tatttaaggg gggggtgggg ttttcgtttt tatccacgcg gtgcgatccc
      241 ctaaggcagt tgccgctccc aaggccccca ctaaaatcgg tggcccccct gcaactcttt
      301 cacgcccccg ccttcacaca aaacccaatt ccccgaaaaa tgtacttgcc gagtcatgct
      361 accttgtagc acaaggtact ttggatacca aggtaccttg gatgacaagg tagcatgaag
      421 caacaagtcg tgagaaggcg agtttgggtg tgagcgagaa tacttcttat attgcccagt
      481 ggatggggca aatgcggcgg gggtgggtgg agacatgcgt ttttaatgtg ctggcgaacc
      541 aggagctgca tggctacggc atcatgctgc gcttgcagga tattccgggg ctgggcatca
      601 cggaggggac cctctacccg ctgctgtcgc ggttgcggct gcaggggctg gtgacggtgc
      661 ggcggtggga ggagtcgccc gatgggccgg cgcgcaaaat ctacgggttg actgcgcgcg
      721 gtcggcaaat tttggaattg atgaacgatc attttgaagt catgcgccgt cagcatcagg
      781 cggcacggaa aggaacccgc ccatgatcca gtggaatccc gacgcgcaac gtgtttttga
      841 tctgcattgc gaggaacaac gcgcggtgct cttggcggcc ggggccgatc cggatgaggt
      901 gtttggcgat tggcgcgcgc tggtggaaac gcgcgccgcg gccgccggcg aaaccgaagt
      961 gcaagtggcg cgggtgcaaa cggaattggc caagttggcg gaagcactgg gcgcattcgc
     1021 agcctcgcgc caggaagcgt ccgcgccact cgcggcgtcc gcgcgcccgg cttggcgggt
     1081 atggttccgg cgtggcgtga cgctgctgct ctggctgttg ggggtggcgt tgccgctggg
     1141 cgtgctggtc ttcgagttga ttgccggttt ctgcgcggag ttgttgtttg atcccatgcc
     1201 gacttggctg cataccctct tgattgcgct ggtgccggcg gcgaatgcat gggcgttgtg
     1261 ggccgcggcc ggccgttgcc aaccacgccc cacgttgcgc atcctgaatg gcatggcggt
     1321 cggcattgcc gccttttatg cgctgcaatt tgccattatt acgcccttcg cggccgttgc
     1381 cattctctac tttggaattg gcttggtacc actggccccc ttgctctcgc tgtgggccgc
     1441 gattgttctg cgcttccggc tgctccggtt gcccgcggaa tcgcggaccc aatttcccgg
     1501 cttgtggcgc acggccctcc ccgcgtttct gctcctgctc gtgctgaccg tgccgcggct
     1561 ggccatcatc cgctacaccc cggacatcga aagcccggac cgcgcggtgc aaattcgcgc
     1621 ggtgcgaatc ctgcgggcgg tcggcgacag agaccaactg ctgcgccagt gctaccggcc
     1681 gctggaaacc tttgatctgt tcgcgatgct ggtggggcat cccttctggc tgggcggggc
     1741 caacggcatt tcccgtgata ccgcgcaagc cgcctactac cgcgtgaccg gtacgccata
     1801 caacgcagtg cccccgccgc gggtcaaggg cttccgcggc caggagttgc tcaatgccga
     1861 ttggtttgat ccggcgctcg ggagcgaccg gatcgcggcg cggatcaaaa atctggcgtt
     1921 ggcccagtcg cggctggatg gccgggtgga tgcggcatcc gggattgcct acttggaatg
     1981 gacgctggag ttccgcaata catcgcccgt gcaacgcgaa gcgcgcgcgt tgatcgagct
     2041 gccgcccggc ggcgtggtgt cacgcgtgac gctctggata aacgacgaac cgcgcgaagc
     2101 ggcttttggc ggacgcagtc aaactcggca ggcctaccaa gaggttgcgg tgcaacagcg
     2161 gcgcgacccg gtgctggtca ctaccgccgg cccggaccgc gtgctggtgc agtgcttccc
     2221 tgtgccggtg gatggcacga taaaaacccg catcggcatc accgcgcctt tgacggtgtt
     2281 gatggccacc ggggccgagg ccatgctacg cctgcccgca ctggtcgagc agaactttgg
     2341 cgtggccgcc aatgtgcaga cgacggtttg gctggaaagc gatgtcccag ccagtgccag
     2401 cgccgggctc agcagcagcg tcggtgaaga caacgtgacc ctccggggca aactggccgc
     2461 ggattccagc gccggcgcgg cccatctggc gctacccatg gccatcccct acccaccgcg
     2521 agtcgccaag gatgagcggc tgcccgacag acaagccatc ttgcaaacgc tcaaacttcc
     2581 cgagacgacg ccctcgttgc cggcggcgct cgcactggtg gtggacggtt ccgcactcat
     2641 gaagtcgcgg gcggcaacca tccaagcgct attggcggca atccctccag aaacccggct
     2701 ctattgcgtg gtggccggcg atgatgccat cgccaccgaa ggcctgccgg gcgcgcgttc
     2761 gttgcggttt gtgggcgggt gcgacaacgg tccagcgctc gcgctggcgg cggagtgggc
     2821 cgcaacccat gattacgcac ccatcctctg gctgcatgcc gcgcaaccgc tgcagtccac
     2881 cgagctggaa gcgctgcgcc aggctgtgga tttctcccgc ggcaaactgg tcatccacag
     2941 cgtacaattt gggccgggtg tggatctcat caccgaaaaa atggcggatt tacgggtcgt
     3001 ccgaccgctg cccgtggttg aggcaactgc cacggacatt ctcgatgcgc tgcgcgatcc
     3061 cggtgccaca tggcaacgcg cactggtcgc cgatgccgac gtgccggccg atgcctcgga
     3121 aggctcatcg cacattgtcc gcctgtgggc ggcggatgaa atcggccaac tcagcgcgtc
     3181 atatcgcaag gggggaacca ctgatgccgt aaaaatggcg cgcacgtttc aactggtcac
     3241 gccggtttcc ggcgccgtcg ttctcgaaac tgccgcccaa tatgccgcgc atgacctgac
     3301 cccggccgac gacaccacca cacccggtat tgtgcccgaa cccggcacat tggggttgct
     3361 gctgttgggc ggctccgcgt tgtgggccat gcggcggcgg aggcgtcgct gacgcggcgc
     3421 cgtaaggtgg cctacctcag caacgccacc ccgatcacca ccccaaaagc aattcggtac
     3481 cacccgaagc cttccaaggt gtgggaggaa atcaggcgca tgagccagcg gatagccaaa
     3541 cgcgccacaa gaaaagatac cgtgatcccg atgatataag gcaaccaatc cgcgcgctgc
     3601 aaggtcggca ggcctttgta gaaatcgtag gcgccggcca gcaggagcac aggaactgcc
     3661 gcaaaaaatg aatattccgc ggcagcccgc cgactcaatt tcagcaaacg tccgccgagg
     3721 attgtggcca tggagcgcga ggtaccggga atcatggcga ggcactgaaa gaggccgatg
     3781 ccgagcgccc gttgccagcc caacgcatcc acgccctcgt cttggagttc cggcagccgg
     3841 cgttccagcg caatcatggc cacgccgccc accatcagcg caccggcgat cacgccgggg
     3901 gtgaatagat attccttgat cgtgccatgc gccagcagcc ccaagaccat cgcgggcagc
     3961 gtggtcagca acagccacca gagaccacgc caaccggcga agccatcggc ttgacgcagc
     4021 ttggccaacc gcacgaaacg ccggcggtat tccaccagca ccgccagcac ggcgcccgac
     4081 tggatgacca catcaaagct ctcgtgaaat gaaacccgca agcagcgttc caccaagatg
     4141 aggtggccgg tggaggagat cggcaggtat tccgtgattc cctccaccaa ccccatcaga
     4201 acagccgaaa ggctggttgc ccatgcactc acgattacca ccggcgccta cagctcaata
     4261 tggaacgcgg tgcgtgcctg aatatcggcg gcaatttgcg cgacgatttc atcactgacc
     4321 ggctgattca ccttgatgat cgccagcgag ttgacacctt tggaatcgtg cggattgcgc
     4381 acatcatcaa tgttgacccc ggctgcggcc aacgctgcgc cgatctggcc caagacgccg
     4441 ggccggtctt cataaatgaa cactacccag ttgccggcgg gctcgacata gagcttgtcg
     4501 aagtcgttga tgcgcgacac aaggaggttg ccctcttcaa cggtgccacg cacgctggcg
     4561 cgggcgaggc gttcggcatc aatgttgccg gtaaggtcaa tcgtgatgga gttggtgtag
     4621 gccttatggt catcagtgac gcggtccacg taatccacac ccatgtcggc caaaaacgac
     4681 agcgtggcct tgtggtcaag cgtgggctcg tagtcagcgc tcaggcccgt ggtgacgggc
     4741 gtcagcagcc atttggcgta aggcttgagc gagccataga aactggtttc caccaatttg
     4801 agctggcgat ccggcccgag aacgcgccgg caaatccgtg ccagcacatt ggcgaggcgg
     4861 ccgaacgcgg ggtccagccc cgccggcaca tcgcggttga ccacggcgct ggtgatgccg
     4921 cgatcgtcca ggtcaatcag ttcctgcgcg gccatttcgg cggccttgcg gttggcctcg
     4981 cgggtgctgg cgccgagatg cggcagcatc aggtcggcaa tatccgccac gcttttctgc
     5041 ccggattcat ccttggcata cacatcgttc aaaaaacgga ttttggcggt ggccttgact
     5101 tcgcgcaaat cggcctcatt gatgactccc gcgcgcgcgc agttgatcag cgtggcaccg
     5161 ggtttcatcc ggctgagcaa agcgcggttc accatgccgc gggtttcctc gttttccggg
     5221 atgtggagtg tcacgtaatc cgcctgcgcg aacagctccg gcaggctggt cagttcaatg
     5281 cccagcgccg ccgctttttc ggcggaggta aagggatcat agccgatcat tttcatctcg
     5341 aatccggaaa ggcgcttggc taccagttgg ccgatatagc ccagccccac gatgcccacg
     5401 atcttgccgg tcaattccgt gcccataaag gcggtttttt cccactcgcc ggctcggacg
     5461 gaagcatcgg cggccaccaa gtggcgggcg tcggccagca tcatggccac gacctcctcc
     5521 gccacggcgt tggagttggc gccgggggtg ttcatgacat ccacgccttt tttgcgcgcg
     5581 tggcggatat caatggtgtt gtagcccgcg cccgcgcgga caatcacctt caaattgggc
     5641 gcggcgtcaa tgatgtccgc cgtgattttt tcgctgcgga caatcagggc gtgcacgtcc
     5701 ggattatcgc gcaccaactg ggccagatca gccttgggca cctgcaccac ggcgtagccg
     5761 ccatgggctt ccagcatcgt tttggccacg gcatccaacg ccgtggggat caacaccttt
     5821 ttcatctctt ttcctttcgt cgtttgaagc agagatgcgc acaacatacg ccgcacaaat
     5881 ggcgcgcgca agcgtcttga cccgctgggg gcgcttgcgt aatgttgccc gcatgttgca
     5941 acccgctttc atccgccgtg gtcttggctg gctgggcctc ttattggttt ggggcgcggc
     6001 catgctcccc cacccggcgc aggcggcggc caaaccctat gtggaaaaag cgcccaagcg
     6061 cgggtttcac ctattcctcc acgccaagca gaaaacgccc accgcccagt gggattatgt
     6121 gcaggatttg gcgcgcgccg gcaaagccaa ggccgccgcc aagcaggcgc tggcgctgcg
     6181 gatttattgg cccaacgccc cggaggcccc gcaggctcaa atgttttatg cccgccatct
     6241 ggatcgccgc caaaatatcc aggatgcttt cgatgcctat caacatcttg tggatcacta
     6301 tcccgggcgc ttcgagtttg acgaactcat cagccgccaa atgcaattgg ccaaaaccct
     6361 gatgaccctc aaaaaggggc attttctgtt catccctgga tttgccgcgc cggagcgggc
     6421 cattccgctg ttcgagaaga tcgtcgccac cgcgccggaa agcccgctgg cggccgaggc
     6481 gttctattgt ctcggcgtgg ccaacgagcg cacctatgaa tatgaaaagg ccattgatgc
     6541 ctacttcacg gtgttgaatc gctttccgga aagcgcgttc gccgaagatg ccgcctattc
     6601 gcaggcgcag tgccacatca agctcgcgca ggagtcgccc aacgacaacc gcgcgctcga
     6661 aaccgccagc gcggcctgca ccttgttttt gcagcgtttt ccggactccg cgcggcgcac
     6721 ggaaatcatc gccagccagg cgcggttgca gcgcctgcag gcggacaacg cttacgcccg
     6781 cgcacgctac tacgaccgca tcctgcacaa acccgaagcc gccttgattg cctatcgcca
     6841 tttcctggcc ctgtatcccc aagccgaaca ggccgccgaa gccaaacagc gcgtggcgca
     6901 gctcacccct gccacgcccc ccccggagaa ctagcatgaa acgcatcgcc ctcattctcg
     6961 tcgcggccat gatttgcctg accggcgggt gcgtccagta ccgattgggc tccatgttgc
     7021 ccgccgacat ccgcaccgtc tatatcccca cctgcgacaa ccaaaccagc gagccgctga
     7081 ttgagcagga tgtcacgcaa tccatcttgt cgcaaatcca gatggatggc tccctgcgcg
     7141 tgaccacgga agacctcgcc gattccgtgt tgcgaatcac cctcaccaag ttctggctgg
     7201 aaccggtggc ctacgaagcc ggcgaaagct ccaccgccaa ccaataccgc atgagcatcc
     7261 gcgcctcttt tgtgttgcag cgcaaggcca ccggcgcggt ggtggtggaa gcgccggcgg
     7321 tgaccggctg gtatgatttc gatttcatgg gcgacatgtc gtcttccaag agcgtggcgc
     7381 tgcgcccggc cgcaaatgat ctgggccggc gcatcgtcaa ccaaatcgtg cagtattggc
     7441 cctagcggag cggcagatga aagaactcga agaaggcact gcgctcacgc tggattttaa
     7501 caagctgacc aaggcggtca gcgcctgtcc aggagtcatc ccggtggccg tgcaaaatgc
     7561 ggacacgcac gaggtcattc tggtggccta caccaacgaa atcgccttga aaaaagccat
     7621 tgccgagcgc acggccattt tctggagcac ctcgcgcaat gaactgtggg aaaaaggcaa
     7681 aacctccggc gaaactttcg aactgctgga agtgcgcgtg aattgcgaac aaaattcgct
     7741 gctctatctg gtgcgcccgc gccggggcgg catttgccac accaagaacc aaaccggcca
     7801 accgcgcaat tgttactacc ggcggttgga tttccaaacc ggtcaactgg tcaaccttga
     7861 cccctgaaag ccgatgacat ccgccgccac acccccgccc cctcccgctc acgatccggc
     7921 gcgcgccgcg gcgaaccggc ggattcttgc gtttgtggtc ctgttcttgc tgctgttggg
     7981 cggctccgtg tggctggtgc tgcatcagga aaaagtgcgt ggccgcttga tccagccgga
     8041 gcaacccgcc gccccagaaa cggaagtacc cgcgccgccc aagccccccc ccgccccccc
     8101 gccccccgtc accaaaccgg tttcaccggc gctcgcgcaa tcggagcaaa tggaagccgc
     8161 actgacggcc taccgcgagg ccggcgagtt tctggtggcc aagcaattcg gcccggcgga
     8221 agatcgcgcg ctggccgcat tgaaggccta tcccaagttg gccggcgcgc aacgcatcct
     8281 gggccttatc tatatccagc agggcaactt tgaccgcgcc atttccgtgc tggaagccgc
     8341 gctgcgcaat gagccattca atcccgaggc gctcaccaat ttggcgtttg cctacctcca
     8401 aacccgcaac acggatttgg cgctggagct catcgaaacc tgtcgccggc tgcatcccga
     8461 ctatcagccc gcgctattgc aggaaggcat catgctgctg agcctgccgc catccgaacg
     8521 cgccgtcacg gtgctgcgcg aagcggtggc cgcttttccc aacatgcccg ggccgcgcaa
     8581 taatctggcc attgcgctcg cgcgcatggg cgaccgcgaa gccgcccgcg aacagttgca
     8641 agtggtgctg gagatggaag ccgacaacgt ctctgcgctc ttcaacatgg gcgcgtttta
     8701 tgcgcaggaa accaatgctc cggccgccgt gccatggctg cgccgcgcca tgcagcatgt
     8761 ggctcccggc agcttccgcg atttcctcaa cgatcccgat ttggccaaca tccgtggcac
     8821 gccggaattt ttgcaactac tgcaagagct ggacccggcc gtccccggcc cgcggcccta
     8881 gcttgcggca gcttgcagtt cggcaagagt ggtccgccct tcgtagagcg ccttgccgac
     8941 aatggctccg ccgagattcg ccttgttttg cgcgcgcagc cggcgaatgt cagccgccgt
     9001 ggtaattcct cccgacgcga taaccatgca cccgggcccg accacatcgc acatctcggc
     9061 cacgccgctg atgttgggac cttgcagcat tccatcggtc gccgtgtcgg tgtagatgat
     9121 ggttttgatc ccgctggccg cggcgcggcg cgccagctcc accgccgtca tgcgcgatgt
     9181 ttccgtccag cccttcacct gcacgaggcc attgcgcgcg tcaatgccca ccgcgagacg
     9241 gtcgccatat ttcgccgcca gttgggccaa ggccgccatg tcctccagcg cacgcgtgcc
     9301 caaaatggcg cggcgcactc cgcaagccag cagcgtttcg atatcttcat cgcgccgcag
     9361 ccccccaccg cattccaccg ggatgccggc cgcctgcacc atcgcagcaa tcaccgcgcc
     9421 atgcaccggc cgtccctgaa acgcgccatc caaatccacc acatgcagcc attgcgcgcc
     9481 ctgccgcgcc cagtccgcgg cttgcgcggc gggatcatgg ccatacacct tcacatcgtc
     9541 cgcccgccct tggcgcaggc gtacgcattg gccatccttc aaatcaatgg ccggcaaaat
     9601 cacgaaatca ctcatcgcgg atcatcccat ctgacgtcat cgcccggaca gcgcgcgcgg
     9661 ttacaaaatg cccttgctgg agggcacgcc tttgacccgc ggatcatggc ggcaggccat
     9721 cttcaaggcg cgcgccaggc ccttgaaaac cgattcccac gcatggtgcg cgtccgcgcc
     9781 atacaattga tgaatgtgca ggttcattcg cgcctgggtc acaaaggctc gcagaaactc
     9841 gcccagcagg ctcaactcga aatcgaggat tttctttttc ttgttggcca tctgcagcac
     9901 cagatacggg cggccgccca aatccagcgc cacgcggctc aaggtctcgt ccatcggcag
     9961 caggctgaag ccgtagcgct cgattccctt gcgctcgccc aaggcttgat tcaacgcatc
    10021 gccgagcgcc aagccgacat cctccaccgt gtggtggtgg tccacctcca gatcgccctt
    10081 ggcccgcaga ttgaggtcca acaatgagtg tttggcaaac agctccagca tgtgattgaa
    10141 aaacggcagg ccggtttcga tctggtacgt gccatggccg tccacattca attccacgcg
    10201 gatttgcgtt tcccgcgttt tgcgttcaat gatggctttg cgtgtcttca tgattccgca
    10261 cgatacccat cccccccgcc ccgcgcaacg gatttcaacg ccccgccccc gcgtccggct
    10321 agggttggcg gcggattttc gcaaagccgc gcttgccgct gcggatcacc aggccgtcgg
    10381 cgggctcgat ttccaagcgg aaatcgctca ccttgtcgtc gccgagtttc acggcgccgg
    10441 cttgcaccag ccggcgcgcc tcgctggtgc tgggagccaa accggctttc accatcaggt
    10501 ggagaatgcc gattttgccg gcgggcagca cgacttcggg aatatccgtg ggcagctcgt
    10561 gctgggcaaa cacccgcgcg aactcggcac tggcctcgtt gccggccgca tccccgtgga
    10621 acttggccac aatgcgccgg gccaactcgt ccttcacctc gcgcggatgc cgcgtgccat
    10681 tggccaccgc cgcgcgcaac gccgcgattt catcctccgg caggcaaagt accagcgaaa
    10741 agtagcgcca catgaggtca tcgctcacgc tcatggtttt gccaaagatg tccttgggcg
    10801 tgtcgttcac ggcaatatag ttgccgaggc tcttgctcat cttgttgacg ccatccaagc
    10861 cttccagcag cggcatggtc atgacaatct gcggctcctg gccatgggct ttttggattt
    10921 cgcggcccaa cagcagattg aagagctggt cggtgccgcc gagttccaca tcggcccgca
    10981 ccatcacgga atcataggcc tgcaccaacg gatacaggaa ttccaccagc gagatgggct
    11041 ggttggcggc gtagcgcttg gcaaagtcgt cgcgagccag caactgcgcc accgtcacgt
    11101 gggcgctcaa ccggataact ttttcaaaca ccatcgggcc gaaccattcc gagttgaacc
    11161 gcacttccgt gcgcgccggg tccaggattt tgaatacctg ctcctgatag ctcttggcgt
    11221 tggccatcac ctgctctttg gagagctgcg ggcgcgtggc cgatttgccg gaagggtcgc
    11281 caatcatgcc ggtgaagtcg ccaatgatga acaccacggt gtggccgcaa tcctggaatt
    11341 cacgcaactt gttcagcgcc acacaatgcc ccagatgcag gtctggcgcg gaagggtccg
    11401 cacccagctt cacgcgcagc ggacgatttt ccgcctgagc cttggccagc cggtttttga
    11461 gttcctccgc gctgtgcagg tccacccgtc gcgcggtgat tttggcaaat tgttggtcca
    11521 gcgtcagcat agcgtcactc aacttccaca ttccagccgt gggtatccgg cagctcgccg
    11581 tactggattc cctgcacggt tttatacagc ttttccagca ccgggccaca gccggtggcc
    11641 ggaccggtct tatagactgt gcccgcccgt tcgatttcgc tcaccggcgt gaccaccacc
    11701 gcggtgccgc aagcggccac ttccgcgaac tcggtcactt cctcaaaagc gatggggcgc
    11761 acttccacgg gaatgccatt atcccgcgca atttgttgca acgtcagatt cgtgatactc
    11821 ggcaaaatgg agcctgattt ggcggtgaca tatttgccgt ccttggtgat tcccaaaaaa
    11881 ttgctcgtgg cgaattcttc gatccacttg tgctccttgg catcgaggta tagctccacc
    11941 ggataacccg cggccttggc ttcaaggtgc ggatagaggc tgaccgcgta atttccggcc
    12001 actttcacgt gtcccgtgcc cagcggcgcc gcgcgatcat attggtccag cacaatcgcc
    12061 ttcacggctt gcaagccgcc cttgtaatac gggcccaccg gcgtcaccag cacgagaaac
    12121 gtgtattcat ccgcagccgc cacgccaatt tgcgcgccgc tgccaaacag cagcgggcgc
    12181 acatacatgg aaccacccgt gccgtatggc ggcacataat ccagattggc ccgcaccacc
    12241 cgcttgcagg catccacaaa catatccact ggcacttcgg ccatgcaggt gcgccgcgcc
    12301 gtcatggcca gccgcttggc gttttcctcg ggccggaaca aactcacctt gccatccttg
    12361 cgccggaacg ccttgagccc ctcgaacgcc gactgcccat aatgcagcgc cgtcgccgcg
    12421 atgtggatgg acatctgcgg cgaagccacc agctcccccg cgctccattg gccatttttc
    12481 cacacgtagc ggatatggca attggtgtcc atgtaggcaa aacccaagct gttccaatca
    12541 atcttcttca tcgttcatcc tctcaaaatc ttgaataatg cgggctcccg ttgggtcccg
    12601 tcagcaaatc ctgcgccacg cgccgcgcaa tggcctcaat ttcgtcgtcc gacagtattc
    12661 cggccaaaca ggccgcgggc gccaccgcgg cggcaactcc actcggcttc gttgcggccg
    12721 gcacccccat tgccgacgcc tgacacatcg ttactgccgc cactcccaca atgtcttccg
    12781 cgctgcaccc acgcgataga tcgttcaccg gcttggccag cccctgcaaa atcggcccca
    12841 acgcaatcgc gcccgccagg cgctccacca gcttcaccga gatattgccc gcctgcaaat
    12901 ccgggaaaat caggatgttg gccgcgccct tgagcgggct gtccggcgct ttttgcgccg
    12961 ccacctgcgg cacgatggcc gcatccgtct gcagctcgcc gtccacgatc atctcggggt
    13021 caacggtcgg ccccactttt ttgatgatgc gcgtggcatc ggccaccttg tctaccaaat
    13081 catgcttggc gctgccgcgc gtggaaaaac tcagcagtgc catgcgcggc ggcgtgccaa
    13141 tcaacgcccg gtgcgtcttg gccgtggcca gcgcaatctc caccagttgc tcgacggtcg
    13201 ggttggggtt tacgccgcaa tcggcaaaca gcaaggtgtt gtcgcccgct ggcgtgggag
    13261 ttttcaggcg cagcaaaaaa caactgctgg ccgttttgtt gcccggcgcc gtgcccacgc
    13321 agtggaaagc cgcgcgcagc atatcagggg tggaggcgat gcttcccgcc accatgccgg
    13381 acgcatagcc ggtttcaatc atcatggccc ccacatagag gcggtttttc atcgcctgcc
    13441 gcgcctgctc ggaagtcaac cccttgcccg cccgcttgcg gtaatacgcg tccaccagct
    13501 cctcaaagca cgggggcgcc gacggatcaa tgatctcaaa cccggcgggt gcaatcccgg
    13561 cggcggcgca ggatttttgc gcttccgccg gagtcgccag cacggatact ttggcgaaac
    13621 ccgagcggac aattgccgct gccgcctgca tcacccgcgg gtcctgccct tccggcaaca
    13681 ccacgctccc gtgaaaacta cgcgccctct caattaacaa ttccataaag aatccatgct
    13741 accgcccccc cttttttccg tccagcaaaa ccgcgccgcg cagcctcagc gcagcgcttg
    13801 caggatgttg agtaactcct gccggctggt tgcggcactg taatgcgcaa cgcaacgcgc
    13861 ccgggcccgc tgccccatgg cggcggcgcc agccgggtcc gccagcaggg cttggatggc
    13921 ttgggccgtt gcagcgacgt cattgggcgg caataaatag ccaccatccc ccagaacggc
    13981 aggcacatct gataccgcgt tggccaagat gggcttgccc atggccatgg cctcaaaaag
    14041 cttgcacggc atttgactgc gcgccagcag cgtatcggtc aacggcacca gcaaggcgtc
    14101 gcccaaaccc agataaagag gcatctcccg atgaatgagt tgaatcgctt cgggggtggt
    14161 ggcagggcaa cacacaagcg ccccgtactc ggaatgctgc tgcaactgct gcacatattc
    14221 attatgaggc cccaaaatca ccacccggca atccgtgcgg cgcaggcgca ccagggcttc
    14281 cagaaatact tccagccctt tgtgcgggcg ggccacacca ccaaacacca acaaggtgtt
    14341 ggcgctcaac cccagttgat gcttgagccg cgcgaccgct tccggatcct ggggctggaa
    14401 acgcgccgtg tccacgccaa tgtgaaaaat ccggcccgcg aatttctgcg caagaaattg
    14461 ggtggtcacc aagcgttcgt cgcattcgcg cagtcgtctt tcgacccacg gcacataaac
    14521 attgttgccc ggatgcatcc aatcgcgcgt ccactgccgc agtcgctcgg accacgacca
    14581 ctccgcggcc accgcgccgt cccactcatc caagtaggcc accacgcggc acccgcgttc
    14641 tcgcttggcg cgcaaagccg ccggcaaatt gctgccaaaa gccttcatcg aaataatcac
    14701 gtcgccggtc agcgcccggg tgagtttgcg cgattcccta aaatattccg gcaagcgata
    14761 caggcggggg caatccacgg gacgataggc gaactccgcc gcatacatga tgttcgcgcc
    14821 tctccacaga ttgggtccca caatctccac ctcatgttcg gggtggagat agttggccat
    14881 gcgcgcggcg atgccgacga tgggccaacc caaatccggg acgagaaagc tgactttcat
    14941 ggggcggcca aaagggtttg ggccagattg cggccgctgg cgtaggcgcg atcggaccat
    15001 acatagtgcc attcgccaaa aaggccggcc gtgacgatgc gttgggtgcg cagccagtcg
    15061 cgcatcaagc gcacggccgg cgcgcgggcc aagtcggaaa ccacgtaaga aagcggatag
    15121 caccggattt cggcggcggt tacctcatcc ggcgcaaagc gcaagatgcg cccaagatcg
    15181 gcaatggcct ggtcccgcaa ttgcgcctcg ttgggccggg cattttgcgg caaaaagatt
    15241 tccgcctgaa tggccgtctg gttgctcgct tggcccgaca aggcaaacgg aaatgacacg
    15301 cgcgaaaccg ccacatccgg gtcataaata taaaaccatt gggccggcga taaagccggg
    15361 cggttgacca ccagattgac gcaatactgc cgcagcgccc gcagtttggc cgcggcctcg
    15421 cgcacggcgg acggggcttc cggcatcatg gccaccaact ccggcaaagg gatcgtggcc
    15481 accagccaat caaacgactc tgccgtgcca tccgcaaacg aaactcgacc ttcgcgcgcg
    15541 gccactttca ccgcgcgttt ttgcaggcgc acgtccacgc catcggccag ccgcgccagc
    15601 atggcaaaaa agccgcccga ctgcggataa cgaaacgtgc ggaacaccgc ttgttgttcc
    15661 gccactggcc cattggcgcc ggcgatgatg ttctccactt gcgaaggaag aagccgcccg
    15721 cccagccaat ccgtggccag ttccgccatg gggacatgcc aatatttggc ggtgtataaa
    15781 cggtaaaagt tttcgagcaa ataatcgcca tattgaaagc ggcaccactc ttcgtagttg
    15841 gccggctccc gaccacgata tttttcctgc gccgcaagga agtcgcgcag cgcggcctcc
    15901 cgctcgggcg ggggcaaatc gaacaaatga ttttgcacgg gataggcaaa ccacttgccc
    15961 tgtttgtggt tcagcaccgt gcttttggtc atcacgtgaa gcggcgcggc cgcatggagc
    16021 aaggccagcc attccgggtc tgacgagtgg cagatatgcg caccgcgatc aaaccacaca
    16081 ccggcaaatg aatgagagcg cgcgtggccg ccgggggtgt cgtccgcctc gaatacacgg
    16141 cagccgggat agtgccgcgc gaagcccagc ccggcgagtc cggcgcccaa cacgatgaca
    16201 ttgcgtttgg tcatgccgct gatctaatca tgaaaaccgc gcaatgggca cggccaaaca
    16261 ttccggacgt acatgggctg gcgctgctca tccgcgcggc tccattgtgg ctttgcataa
    16321 ctcggcaatg ccccgctgcc atgaaatctg cgcacgccag cccagcacgg cggccgccgt
    16381 ggcgacatcg gccacttgtt cgggcggttc gttggggcgc atggggcgca caccaaaccg
    16441 gaacaactcc gtggcctggg tcaactcccc gatgcgctcc aacacatcgc gcaagcgcac
    16501 gggttcgccg cggcccaagt tgcaaaccgt ccacgatgtt ttcagggccg gcaggcccag
    16561 cgtggccgca acaattcctg ccaccacgtc gtctacatga acaaagtcgc ggagttgatg
    16621 gccggccgaa aagtcgccgg ggatacgttc gcgggcgcag cgcaaggcaa agggaatcgc
    16681 catggagccg ccttgtccgg ggccatagac aacaaacggc cgcagccaca ccgccttgcg
    16741 gttcgcggcg cgcgcccaag cagccagcgc ctgcccggct gcgaacttgg cttgcccata
    16801 ggcggagagc agcggccctg gcgcctgatc ctcgcgcagc cgcccagcga ggtcgccgta
    16861 ctcctcggcg ctgcccaacc ccacaacccc gcgccatgat ccggcggaca acagccgctg
    16921 gaagtcggcc aacgcctgag tctgcccggc agcatccgcg cgcgccatgt tccaagccaa
    16981 atggaccagt gtgccctcgg tcaccggggg cgcgtccgtg gaaagatcca cacaatagtg
    17041 ttcaaccccg gcaagcggtt ccccgggcca tggcacccgg tccagcgccc acacgcggca
    17101 tccggcttgc gccaaacggg ccacggttcg ccggccaata aagccggccg cgccagtgac
    17161 gacaatcggg gggcttgcgc tcatcccatt tttccacgct gtggaaaaaa gttttccaca
    17221 ctgtggaaaa cttttagccc gcccatggcg cagacccgga ctcgtagatt ttttcgagat
    17281 tttcgcggtc gcgcatggtg tccatgcaac cccagaatcc ctcgtggcgg tacgcataca
    17341 actcgccggc agcggcaagg tcggttagtg gcgcggcttc caagctgacg ttttcctgcg
    17401 agggcaggcg cgccaagaat tctttttgca ggaggaaaaa gccgccattg atgtaatcat
    17461 tgtgcaattt ttccttttcc cggaacacgc gcactttgcc gtccggggcc agctccatca
    17521 tgccgaagcg cgagggcggg tgcacggcgg ccatggtgcc gattttgccg tgcgcgcgat
    17581 gaaaagccaa ttcggcgttg agatcaatat cgctcaggcc gtcgccgtag gtcagcgcga
    17641 acaggggggc atccacataa tctgccgcgc gtagaatccg ggtggcggtc aggctgtgtt
    17701 cgccagtttc cgccagaatg atgtcccagt cggtggcaat gccggattca tattccaccc
    17761 ggccacccgc gtgcaaccgc acggtcgcgg tgcgttcgcg cacgtgaaga aaatactcct
    17821 tgatgacatc gcctttgtag ccgaggcaaa tgatgaattt gcgaaaaccg aagcggctat
    17881 aatactgcat caggtgcagc agaatcggtt tgccgccgat ggtgaccatc ggtttgggaa
    17941 tatcgcgcgt ttcttccatc aggcgcgtac cgcgcccacc cgcgagaatc atgacgggaa
    18001 tgtctttgtt ctgcataatc ggctcccttt aggttgcggc ttcgtaagcg gcaatttcgt
    18061 cgttcataaa agcgcggaca gcctccacgt tgcgcgcggt cagcatggtt cgatagcctg
    18121 ccgcagtgcg ttgcacagtg gtttcaaaat tccatttggg gtgccaggcc aagcgctcgc
    18181 gcgccttgtc tatggccagt tgcaacaagt gggcttcgtg agggttggcc ggattgggca
    18241 tggcttgcca ggtcgcgcca tcgccccagg cgcggcaaaa caattccgcc agttcgccca
    18301 cattgcgcgc actggcagga tcagggccaa agttccagcc ttcggccgcg cgcgcgcctt
    18361 gctccagcaa ggcttggccc aagcgcaaat agccgctcaa cggttcgagc acgtgctgcc
    18421 agggacgcac cgcgcgcggg ttgcgcacgg acaacgcttt gccggcacga atcgcccgca
    18481 cggcatcggg gactatgcgg tcggccgccc agtcgccggg gccgatcaca ttgcctgcgc
    18541 gggctgtggc cagatgaacg cggccgggcg ccgcaaaaaa ggcgcgccgg tacgatgccg
    18601 cgaccaattc cgcgcagccc ttggaagcgc tatacggatc gtggccgccc atgggatcct
    18661 cttcccgata ggcgtaatcc tcttcgcggt tttcgtaaca tttgtcgctg gtgactacca
    18721 ccaccgcgca gggttggccg gcttggcgaa tggcttcgag cacatgggcc gtgccgcgga
    18781 cgttggtatc gaatgtctca accggttcga cataagaacg acgcacgaga gcttgcgcgg
    18841 ccaaatgaaa aaccacctcg ggctgaaaat cgcggacgag cgtcagcatg cgcgcggcat
    18901 cgcggatatc cgcggtttcg tggcgaatga ggtgcggcaa ctcggcccgc acaaacggtc
    18961 cctccgcggt gtcgggctcc agtccgatcc caaacacctc cgcgcccagc cgatgcagcc
    19021 aaaaagccag ccatgatcct ttgaagccag tatcgccggt cagccagaca cgtttgccgc
    19081 ggtaggtttc gccaaatgtc attacgcgag cctccgtttc agaatacgcg gcacttgccg
    19141 cgccaagcgc caccagcgcg ccccggccgc ataccagggc gatgttcccg gcaaattgcg
    19201 cgcacgaata tcttccaaca aagttgagcg atccaaccaa tcctgttgca caaagaatga
    19261 atagtgcaac accaccatgc tttggtcgag aacgcagata tgttttttct cctcgaacca
    19321 agcgcaccag tcgggctcat cccgcggtgg caaggcgccg cccatgcgtt gcacgtgtgc
    19381 gtaatcgtag cagatgcagc cgatgctgaa gtattggcaa aactcatgcc agcgtggctg
    19441 atcggatgcg gccagtcgcc ggcgctgctc ctcattggca gcttccagat caagaaacag
    19501 acgagtggcc cattcggcaa tgtccgggcg ctgccaagtg ggacctttgg cggcagcgga
    19561 tggcggtgca ccaaaaagtc gttcaaattc cggaagtttt tcaggataaa acgtcgtcag
    19621 caaacgatgc aggccataag cattgttggg gatcaatgtg gtgattagcg ccagatcggt
    19681 gcggtcttgg tagcgttcgt atgtggcgag catccgttcc gcccaacccg tggggacaaa
    19741 gacatcctca tccaccttca cataaatgga atccggataa cgccgaatgc attcattttg
    19801 taccgcgcaa ataccgcccg gtcgggtgcc gtccccgtcc actgtatccc agcgcacttg
    19861 gggccgcgcg gccatgaatt gctgaacata acgccgtaat cgcgccggca ccccgttgag
    19921 cagcaagacc acatgatcga atgccgcaaa cgcattcgct ttttccagca tctccaagca
    19981 cagccgcaga caatccaaac ggtcatgggt caggatcatc aaaaccttac ggcgtgattt
    20041 catgggcatg agtatagcac ccctgctggc ggaagcgtta gcgaaaccat gcgcagttat
    20101 aattttcggc ggtcgggttg ggcataagcc aacgggcggc tcgacacacg cggcgcgatt
    20161 cgaaacaagc atgcacttgg gcaaacaaca ggccccacga cgcccccctc aaaaaaaaag
    20221 aaaggccggg ctgcatgggc agtccggcct ggtaaaaaaa tcctggcaac gtgctactct
    20281 cccgccccca agtgaggcag taccatcgcc gcagcgagtc ttgacttctg tgttcggaat
    20341 gggaacaggt atgaccctcg cgc
//
LOCUS       DAOPNP010000037        19569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3407_length_19569_cov_8.458286, whole genome shotgun sequence.
ACCESSION   DAOPNP010000037 DAOPNP010000000
VERSION     DAOPNP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19569
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_19569_cov_8.458286"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKI64_04945"
     CDS             <1..387
                     /locus_tag="PKI64_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00392.1"
                     /translation="NAQRKPILFCLLILSVEKKFIPRGRDFPHRGKTGLFFPRNGKTL
                     RDFSTQWNNFEVIFPHCGKIISTLWKRRCPRSEPPQGGGGVQEKTARPKLPWQAGEGQ
                     WLRAGRGIGRIGRMRQDRRMDVFLEP"
     gene            complement(428..>1522)
                     /locus_tag="PKI64_04950"
     CDS             complement(428..>1522)
                     /locus_tag="PKI64_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00393.1"
                     /translation="ATVADQPTAAPAPAEAALEPEPLSASTGTVTPGAWNHPGFIQAA
                     TLFNETLTAYQAALRNPDPQNPALVGLIADAQYAALLFETIRPQAPAGVPILQYANQC
                     YQLVNDLRRARLTEDDKRNLYALAPKKRHPTLAPYPTPAPDPSDTFAPRYMQFGYAWD
                     TLPAPVDRPETAEFVFLLSTIAEATPDTRAQPGLFIHGPLNWLMPAADAIRAIGGKAD
                     IQRVPIEGAPFPYGGCFLRAYPAGRYGMPGPGQPPYPTLRLITDSDDQLIAVQLVDEN
                     PSPPLQSSPMTFTTTSKIMDFVTGRVLSEKGDTRATHRTLKGAGTLRIDSEAADFTSG
                     PDGKPLYRSILLLPQAFANNLLYHLLGSAN"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1623..2560)
                     /locus_tag="PKI64_04955"
     CDS             complement(<1623..2560)
                     /locus_tag="PKI64_04955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE00394.1"
                     /translation="MESRSRQHAPPAVPGYEIMSFLGAGGTSTVWLARQLSLDRVVAI
                     KVLSPALLTDDTAREQFQKEARAAAKLSHPAIVGIVDFGEHDGALFYIMEYVDGTNLA
                     QWVAQNQRMAHGQALKLVNIIAGALDTAWRETGLIHCDIKPGNILLGVDGAVKITDLG
                     LARIAGVAATREASEIIEGTPTYIAPEQIEGRQPDCRSDIYSLGLTLYHILTGKPPFE
                     GRSLNEVIEAQQNDYLPDPCEMLPHLPVSYGWLLAKMTAKDPAKRHQSWADVLKDIRQ
                     IQGGMSPLPPYPGEDESTILLNPRHRPGARAHTINLS"
     gene            2726..3151
                     /gene="tsaE"
                     /locus_tag="PKI64_04960"
     CDS             2726..3151
                     /gene="tsaE"
                     /locus_tag="PKI64_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017304188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOE00395.1"
                     /translation="MTELETTSAAATQAFAAEWAATLPPGTVLCLHGELGAGKTCFVQ
                     GLAKGLGVRRPVGSPTFTLINEYRGRLPLAHIDLYRVRGSADAFGLGLEDYLFHFDGV
                     VAIEWAERAADLLPETCWHVRISAGTAPDHRRIVLERPA"
     gene            3231..3902
                     /locus_tag="PKI64_04965"
     CDS             3231..3902
                     /locus_tag="PKI64_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOE00396.1"
                     /translation="MKIVKDGYSLIIGIPVVCVVAALIVQNYAPAAVGAAWTLYALGL
                     AGCLFMLYFFRDPERTPAGGPDEFVSAAEGVVRSVEFMEEPRYLKCPAVRISVFLNPF
                     NVHVNRHAMSGVIREAGYTPGRHLFTMDPRSSEFNEHSSILVEGEKTRCLQRQIVGPI
                     VRRVVYWQDAGARVEKGQRLGMMKFGSRMDVYFPAADVEATVQRGDRVRAGETVIARL
                     KKGGA"
     gene            3902..4687
                     /locus_tag="PKI64_04970"
     CDS             3902..4687
                     /locus_tag="PKI64_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOE00397.1"
                     /translation="MPAWLRKAIPSACTFLALCAGVGSMLASGQGKFLLAAQLILVSL
                     ILDGLDGTLARLLRSQTSFGAELDTFVDITSFGLAPAVLLYFYPGGLHTLPGWGLVVT
                     FLYAMSGAARLARFRVVDPYRGQRGFLGLPITTAAGFTAAYLIALESPAATRLSWLSL
                     REGPLAIGFWAAILLMLVLQVSHIRYSKPTKNPRVLMPFMVCVVMLFIEPVAIYSAGA
                     MFAYGVWFAFISPFFFRRFLAHLPPPDEMVPVAEAEHAEEDLP"
     gene            4684..5166
                     /gene="coaD"
                     /locus_tag="PKI64_04975"
     CDS             4684..5166
                     /gene="coaD"
                     /locus_tag="PKI64_04975"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOE00398.1"
                     /translation="MSSAVYAGTFDPLTFGHLDVAARAAQVFSELVVAVAEDPRKTLL
                     FSAEERMELARASLAHLPNVKVELFGGLLVDWARRRGIRTLIRGLRAFSDFEYEFQMA
                     LTNRKLAPDIETLFLMPNEDFSYISSSMVREIASLGGSVAQFVPDVVATALHRKLTPK
                     "
     gene            5215..5730
                     /locus_tag="PKI64_04980"
     CDS             5215..5730
                     /locus_tag="PKI64_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOE00399.1"
                     /translation="MIRLATSQIGLDGYRVDGEESGELLDLANDPVARAAGAIQYDLT
                     VTRAGDELVVQGTVAAPLSLRCSRCAQFFSTTVRVSSFLHAYESAKHPEFLDVSDDVR
                     EDLLLEIPGFPLCRDGCRGLCAQCGQDLNESPCGCAPPEPEASPWAVLDQWKAAPGSP
                     PPRGRASQGRT"
     gene            5746..5937
                     /gene="rpmF"
                     /locus_tag="PKI64_04985"
     CDS             5746..5937
                     /gene="rpmF"
                     /locus_tag="PKI64_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005376859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOE00400.1"
                     /translation="MAEPKRKVSKSRVRMRRAAWAGKIPKAATQECPECGAPRQPHRV
                     CPSCGKYKGRQVIVKATKD"
     gene            5977..7002
                     /gene="plsX"
                     /locus_tag="PKI64_04990"
     CDS             5977..7002
                     /gene="plsX"
                     /locus_tag="PKI64_04990"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOE00401.1"
                     /translation="MRIAVDAMGGDYAPQAIVEGALLAVRECPDIKCLYLVGDEQAIA
                     AEIARCGGAPDRVQVRHASEVIGMGDSPATAVRRKKDSSINRAMDLVKNGEADAVFAA
                     GNTGAAVASATLKMRTLEGIRRPGIAVAMPTPAEKPVLLIDGGATPDCTPEILMQFAI
                     MGTVYSQKVLRQATPKVGLMSVGTEEAKGNALSKETFELLECAPIHFMGNVEGHDLFE
                     GVVDVVVCDGFTGNVILKTSESVAHAIKKWLRRELVRNPLRWLGTLLLWGGLRSLRRK
                     TSQKTYGGAPLLGVNGICIIGHGSSDKIAAKNGILQAVQAVKLRVNEAIVAGLQKCAP
                     ASEGTPA"
     gene            6999..7991
                     /locus_tag="PKI64_04995"
     CDS             6999..7991
                     /locus_tag="PKI64_04995"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOE00402.1"
                     /translation="MTKTIHAAITGVGHYAPAKVLTNADLEKMVDTSDEWIRTRTGIR
                     ERRIAANDEMTSDMGVAAARAALAQAKLDPADVEAIYVATCTPDMIFPSTACLIQAAL
                     GAKRAYGFDISSACAGFIMALDTAAAAVESGRVRNALVIGAEKLSVVTDWTSRDTCVL
                     FGDGAGAVVLQPSPRPGLRSSLLGVDGNQAEILYIPAGGCKQPSSEETVRAHRHAIHM
                     SGRETFKIAVNTMQEAAQEAIARAGLTADQIDCMIPHQANYRIVDAVAKRLGPNVMDK
                     VFLNLDRYGNTSAASIPIALSEAVAAGKVKSGDKILMVAFGGGLSWGGAVVEWL"
     gene            7988..8914
                     /gene="fabD"
                     /locus_tag="PKI64_05000"
     CDS             7988..8914
                     /gene="fabD"
                     /locus_tag="PKI64_05000"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOE00403.1"
                     /translation="MSTMLLFPGQGAQAVGMGQDLAAGLPECRALFTQASEVLGYDLL
                     KLCAEGPIEELTKSHQAQPAIFVVSQAARIALAHALGGKLAVAGTAGHSLGEWAALHE
                     AGVISFADAVRVLRARGQFMQEACEATPGGMLTLIGLPLDKVQEVAQASGLEVANLNS
                     PVQTVLSGHADRIAAGEAAAKAAGAKRALRLTVAGAFHSSLMQPAADQLAAFLQDVEF
                     RAPRCPVWSNVTGKPHTTPDEIRRLMVAQVVSSVRWTDAYADMAAAGMKVGYECGPGT
                     VLAGLGKRIVPEVAIASIFDLAGAQEAAKLAV"
     gene            8940..9677
                     /gene="fabG"
                     /locus_tag="PKI64_05005"
     CDS             8940..9677
                     /gene="fabG"
                     /locus_tag="PKI64_05005"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOE00404.1"
                     /translation="MLENKVAIVTGAARGIGQAIALQLAADGADLALGDVKVEWLAET
                     AEKVRALGRRAECYAMDVANSAVVNEVVTQMAADFGHLDVLVNNAGITRDTLLIRMTE
                     EDWDAVLDINLKGAFLVTKAVAKIMMKQRSGSIINIASVVGVMGNPGQVNYSASKAGL
                     IAMTRTLAKELGSRNVRLNAVAPGFIRTAMTDKLSEQAKEYMLRNVPLGRPGEPEDVA
                     KAVAFLAGDNASYISGQTLPVCGGMIW"
     gene            9714..9953
                     /gene="acpP"
                     /locus_tag="PKI64_05010"
     CDS             9714..9953
                     /gene="acpP"
                     /locus_tag="PKI64_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOE00405.1"
                     /translation="MALEDKVRDIIVEQLGVNKEQVVPTASFIEDLGADSLDTVELVM
                     AFEEEFGASIPDEDAEKLTSVGKVIEYLKEKGFDK"
     gene            10060..11304
                     /gene="fabF"
                     /locus_tag="PKI64_05015"
     CDS             10060..11304
                     /gene="fabF"
                     /locus_tag="PKI64_05015"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOE00406.1"
                     /translation="MAQQRRVVVTGLGVVSPVGSTVDSFWAAILAGQSGIGPITYFDA
                     SAFDTKFAGQVKGLNLDDFIPKKEQRRMDPFCHYGVSAAKMAMADSGLDMAKEDPTRI
                     GVIAASGVGGLQILQAQMDILRSRGPGRFSPFMIPQMITNILPGLISINHGLKGPNFA
                     VVTACASGTHCIGEALNLIRCGAADAFVAGGAEGAICELGVGGFNAMRALSTRNDSPT
                     TASRPFDKDRDGFVMGEGAGMLVIEEYEHAKARGARIYCELAGFGCTGDAFHITAPDE
                     SASGPSRGMMLAMQDAGVTTAEIDYINAHGTSTELNDAGETKAIKIALGEADARRTAI
                     SSTKSMTGHLLGAAGGVESVACCLAIRDSVLPPTINYTTPDPACDLDYVPNEARPGKI
                     RACLNNSLGFGGHNATLCFKAI"
     gene            11384..11983
                     /locus_tag="PKI64_05020"
     CDS             11384..11983
                     /locus_tag="PKI64_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE00407.1"
                     /translation="MATAKQNTKRRQEQIAQATLALVAEHGLAACNVAAVARRVGLVP
                     SALYRHFRNKDHMLDAAVEVIQSKLLDNVRAVCARQAHAIERLHDLLQQHVQLIKKSR
                     GIPQVIFSPDFYVDRPQRRARVCDGIRQYLAAVADMIREGQATGTIRPGLDAETAAVL
                     FLGLVQPSAVLWNLSSGKFNVARHAEKAWPLFVQILTTG"
     gene            12125..13279
                     /locus_tag="PKI64_05025"
     CDS             12125..13279
                     /locus_tag="PKI64_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE00408.1"
                     /translation="MKKKVRLILIVLVVLVGAGVAWRLTSQRNRHTDRIRVSGNMEVS
                     EVELSFRIPGWVAARVVDEGMAVNAGDEVARLDATELTHEVELRKHEVAAAQAFLAEL
                     EAGSRPEEIAEAQALAAQAQARLAELEAGSRPQEIAAAQAAVARAQADAANRQGDLNR
                     LTQLHQSGSVTDQELDSAQTASAMAAARLQEAQEQLRLVEEGPRVEQIEQARAGLAQA
                     QERLALVQKGPRQETIAQARARMEQAEQTLAIAQTRLAYATLQSPVSGLVLAENIEAG
                     EFVTAGTPIVTVADLSRIWLRAYINEPDLGRVKVGQPVRVFTDTYPGKAYPGRITFIA
                     SAAEFTPKNVQTSQERVKLVYRVKITVDNPELELKPGMPADADILLNGGE"
     gene            13292..14227
                     /locus_tag="PKI64_05030"
     CDS             13292..14227
                     /locus_tag="PKI64_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00409.1"
                     /translation="MDAIRVEHLAKSFGDLHAVRDLSFAVAPGEIFGLVGPDGAGKTT
                     TMRLLTSIMEPTAGAAWVAGHHIVDEAERIKEKIGYMSQRFGLYPDLSVEENIHFYAE
                     IYGVSRRGRAEKMERLLAFSNLTPFKKRLAGNLSGGMKQKLGLVCALIHTPQVLFLDE
                     PTNGVDPVSRRDFWRILYQLLRDQVTIFVSTAYLDEAERCNRLALLDHGRMIGLGTPD
                     EIKRLMRGAILEVRAANPRRACEVLRAELGTAAVGLFGDRVHVVTRNPADMPQQIRHT
                     LQQAGLTATGIRPVDPSLEDVFVSVLAARDEDTAS"
     gene            14224..15198
                     /locus_tag="PKI64_05035"
     CDS             14224..15198
                     /locus_tag="PKI64_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00410.1"
                     /translation="MTLSAPKPAVVVHELERRFGRFVAVNKVSFTVNQGEIYGFLGPN
                     GAGKSTTIRMLTGILAPSGGSGTVAGFDIRRETEKIKTQIGYMSQKFSLYDDLTVEEN
                     IDFYSGIYRIPAAKKSARKQWALQMAGLTEHRHSLAAVLSGGWKQRLALGCAVLHEPP
                     ILFLDEPTSGVDPASRRQFWNLIYELAGQGVTIFVTTHYMDEAEYCDRIGLIYRGELI
                     AEGTPEQLKNEWMQEDVLDLQCDRPQDAMALIETLPGVKEVALFGKGLHVVATHSETA
                     LAELKTALPPAGFAIQRLEAITPSLEDVFVSLIEARDRADADSREVRR"
     gene            15213..16346
                     /locus_tag="PKI64_05040"
     CDS             15213..16346
                     /locus_tag="PKI64_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00411.1"
                     /translation="MVFKRIYAVARKEMLHVVRDPRSLGMAIAIPMLLLVLFGYALTL
                     DVDNVPLVVWDQDETPASRELVSRFLGSHYFSLGEYARDYRQVERAVDTGKSLAGLVI
                     PRGFAGGIAAGRETPVQLLVDGSDSTTATLAMGYADTVVRAYSQDIVLRQAQRTGGVA
                     RRVPLDLRPRVWFNADMESKNYIIPGLIAVIMMVIAALLTSLTVAREWERGTMEQLIS
                     TPIKGWELVTGKLLPYFLIGLLDVALAVLMGEFLFQVPLRGSVALLFTLAAVFLAGAL
                     ALGLVISIVTKSQLLASQLAMVLTFLPSFLLSGFMYAISNMPRPIQLVTYTIPARYFV
                     ALLKGIYLKGVGLRILLLEAGLLTIFAVLIVLLAVRKFRKKLG"
     gene            16352..17482
                     /locus_tag="PKI64_05045"
     CDS             16352..17482
                     /locus_tag="PKI64_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00412.1"
                     /translation="MFERIVHMLHKEFRQIFRDPKMTRIIFLMPVIQLLVFGYAVTTD
                     VKHVRTAVCDLDNSVASRELVARFVHSGYFTITARVETEQEIRELMDRGQVQAVLQIN
                     HGFGADVQAGKTAPLQIIVDGTDSTTAGIVLDYSAKITGLYSTQIIRHQTERLAGAAP
                     TPGTVALQTRAWFNDNLESRNFYVPGVIAIIVMLITLMLTSMAVVREKEIGTMEQIMV
                     TPITPTEFILGKTVPFALIGFADVVLVAVVGVFWFAVPIRGSLPLLFGATALYLMTTL
                     GIGLLISTVSQTQQQAMMSTFFFYFPAVLLSGFMFPIANMPTVIQWLTYLNPLRYYLV
                     VIRGIFLKGVGPGILWPQMLALGVMGVCTLWLASRRFNKTMA"
     gene            17499..18917
                     /locus_tag="PKI64_05050"
     CDS             17499..18917
                     /locus_tag="PKI64_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE00413.1"
                     /translation="MKKNRIKPLWVLAAATALLMAGGCANPLLTPAVPDIADYLAASP
                     SPEAPTADGDVAVAGAPLELAACVRIALADNPVGQAAREGVAAARENIGIARSRFYPQ
                     VGLTGSYRRWESHAFLPDSLSGAMASTPATVGPTDDWSAGGVASFYLFDSGRRRAQLL
                     AAKAQWGAQQAEQAAVEQDIALNVHQAFYSLLAAQESLRVAQENSARAEDHFRLTQDR
                     KSAGMAVQADVLRAQVEVADRHLAIVRAEHGVRVARGNLNLAMGLPAEMPVEIDSAAP
                     GAPVEPAPLADDLATAMEQRPELRAALNRVASARSGVSAAKSEFGPVVKADARYGWRD
                     TEFTPEDKDWLIGVGIELPLFTGFSRTHQLQRARHELSKEEALVRQLALTIRQEVWDA
                     HSRVQEAMEALDAAGTLVASARESQRLSRQRFEAGTNTITDLLDAEAAQARAEGLQVE
                     ARWNYFISRAALARAIGAMDTK"
     gene            complement(19220..>19569)
                     /locus_tag="PKI64_05055"
     CDS             complement(19220..>19569)
                     /locus_tag="PKI64_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOE00414.1"
                     /translation="CKSALYEFDGSNPSPTTISESAMQELSQILNQIPTDPGVYVLLG
                     QNGLRYTGAARNLQARLRDHHAGRGKRTHNQRPLKLLHIETSGDFSAALKREAYLKSG
                     KGREWLNQQTVSP"
BASE COUNT         3520 a         5894 c         6411 g         3644 t
ORIGIN      
        1 aacgcccaaa ggaaaccgat cctcttttgc cttctcatcc tatctgtgga aaaaaagttc
       61 atcccgcgtg ggcgggattt tccacaccgt ggaaaaacgg ggctgttttt cccacgcaat
      121 ggaaaaactt tgcgcgattt ttccacacaa tggaacaact ttgaggtgat ttttccacac
      181 tgtggaaaaa taatttccac actgtggaaa agaagatgtc cgaggtcaga accgccgcaa
      241 ggcggcggtg gggttcagga gaaaaccgcc agaccaaaac tgccgtggca agcaggagaa
      301 ggccaatggc tacgcgcggg gcgcgggata ggacggatag gacggatgag acaggatcga
      361 cggatggatg tttttctcga accctgaact ctgaaccctg aaccctttga gcggatgcgg
      421 ggtgcggcta gttggcggag ccgaggaggt ggtagagcag gttgttggcg aaggcttggg
      481 ggaggaggag aattgagcgg tagaggggtt tgccatcggg gccgctggtg aagtcggcgg
      541 cttcggaatc aatgcgcagg gtgccggcgc ctttgagggt gcggtgggtg gcgcgggtat
      601 cgcctttttc gctcagtacg cggccggtga cgaagtccat gatcttggaa gtggtggtga
      661 aggtcatggg cgaggattgc agcggcggcg aggggttttc atccacgagc tgcacggcga
      721 tgagttgatc gtcgctgtcg gtgatgaggc ggagggtggg gtaggggggc tggccggggc
      781 cgggcattcc gtagcggccg gcgggatagg cgcgcaggaa gcagccgcca taggggaagg
      841 gggcgccttc gatgggcacg cgctggatgt cggctttgcc gccgatggcg cggatggcgt
      901 cggcggcggg catcagccag ttgagggggc catggatgaa aaggccgggc tgggcgcggg
      961 tgtcgggggt ggcttcggcg atggtggaaa gcaggaaaac aaactcggcg gtttcggggc
     1021 ggtccacggg ggcggggagg gtgtcccaag cgtagccgaa ttgcatgtag cggggggcga
     1081 aggtgtcgct cggatcagga gcgggggtgg ggtagggggc gagggtgggg tgccgtttct
     1141 tgggggcgag ggcgtagaga ttccgcttgt cgtcctcggt caggcgagcg cggcgcaggt
     1201 cattgacgag ctgatagcat tggttggcgt attgcaggat ggggacgccg gcgggggcct
     1261 gcgggcggat ggtttcaaag aggagggcag cgtattgggc gtcggcaatc aggccgacaa
     1321 gcgccgggtt ttgcggatcg gggttgcgca gggcagcctg gtaggcggtg agggtttcgt
     1381 tgaacagggt ggcggcctgg ataaagccgg ggtgattcca cgcaccgggg gtgacggtgc
     1441 cggtgctggc ggagagcggc tccggttcga gtgcggcctc ggcgggggcg ggcgcggcgg
     1501 tgggctggtc ggcgacggtg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nngaaaggtt gatggtgtgg gcgcgcgcgc cgggccggtg gcgcgggttg agcaggatgg
     1681 tgctttcgtc ctcgccggga tacggcggca acggggacat gccgccttgg atttggcgga
     1741 tatccttcag cacatcggcc cacgattggt ggcgcttggc ggggtctttg gcggtcattt
     1801 tggccagcag ccagccgtag gaaacgggca ggtgcgggag catctcgcac gggtcgggca
     1861 ggtaatcgtt ttgctgggcc tcgatgactt cgttgagcga gcggccctcg aacggcggct
     1921 tgccagtgag gatgtggtag agggtgaggc cgagggaata aatatcgctg cggcagtcgg
     1981 gctggcggcc ttcgatttgc tccggggcga tataggtggg ggtaccttca atgatctcgc
     2041 tggcttcgcg tgtggcggcc actccggcga tgcgcgccaa ccccaagtcc gtgattttga
     2101 ccgcaccatc cacgccgagc aaaatgttgc cgggtttgat atcgcagtgg atcaggccgg
     2161 tctcgcgcca ggcggtatcc agcgcgccgg cgatgatgtt caccagcttg agcgcctggc
     2221 cgtgcgccat ccgctggttt tgcgcgaccc attgcgccag attcgtgccg tccacatatt
     2281 ccatgatgta gaacagcgcg ccgtcgtgct cgccaaaatc cacgatcccg acaatggccg
     2341 gatgcgagag tttggcggcg gcgcgggctt ctttttggaa ctgttcgcgt gcggtgtcgt
     2401 ccgtcagcag cgcgggggag agaaccttga tcgccacgac gcggtcgagg gataactggc
     2461 gcgccagcca cacggtggag gtgccgccgg cgccgaggaa cgacatgatt tcatagcccg
     2521 gcacggcggg cggcgcatgt tgacgggacc tcgactccat ggcccgtcat tctggacaaa
     2581 accgtggcga aagaaaactg ctaaattgct gcgcgcgcaa gtgtgcgctt gccaagcggc
     2641 gcgctttgcg gtgtgctgct gggctcgcgg ccgagtgtcg gccgcatcac acaggatgat
     2701 gcacccggtg accacacaac ggcaaatgac tgaactggaa accacatcgg ccgcggccac
     2761 gcaagctttc gcggcggagt gggcggccac gctgccgccc ggcaccgtgt tatgcctgca
     2821 cggcgagctg ggcgcgggca aaacctgttt tgtccaaggg ctggccaagg ggctgggcgt
     2881 gcgccgcccg gtggggagcc cgacgtttac gctcatcaac gaataccgcg ggcggctgcc
     2941 gctggcgcac attgatttgt atcgcgtgcg cgggtcggcg gatgcgttcg ggctggggct
     3001 ggaggattat ctgtttcatt tcgatggcgt agtggccatc gagtgggccg aacgcgctgc
     3061 ggacctgctg cccgaaacct gctggcatgt acggatctcc gccggcaccg cgcccgatca
     3121 ccggcggatt gtgctggaac gaccggcctg aacgggccgc gcccgccccg ttttgggcgg
     3181 aaaagtgttt gcgcggtggc gcgggggggg gtaggatgcg gaacttggtt atgaagattg
     3241 tcaaagacgg ctattccctg attatcggca tccccgtggt gtgcgtggtg gcagcgctga
     3301 ttgtgcagaa ttatgccccg gcggccgtag gcgcggcgtg gaccctctat gcgctggggt
     3361 tggccggctg cctgttcatg ctctattttt tccgcgaccc ggaacggacg cccgctggcg
     3421 ggccggatga gtttgtttcc gcggcggagg gggtggtgcg ctccgtggag ttcatggagg
     3481 aaccgcgcta tctcaaatgc ccggcggtgc gcatcagcgt atttctcaat ccgttcaacg
     3541 tgcacgtgaa ccgccacgcc atgagcggcg tgatccgcga ggcgggctac acgccgggac
     3601 gtcacctgtt caccatggac ccgcgctcct cggaatttaa cgagcacagc tcgattctgg
     3661 tggagggcga aaagacgcgc tgcctgcaac ggcagattgt cgggccgatt gtccgccgcg
     3721 tggtctattg gcaggacgcg ggcgcgcgcg tggaaaaggg ccagcggctg ggcatgatga
     3781 agttcgggtc gcgcatggat gtgtatttcc cggcggcgga tgtggaggcc acggtgcagc
     3841 gtggcgacag ggtccgcgcg ggagaaacgg tcatcgcgcg gttgaagaaa ggcggcgcgt
     3901 gatgccggct tggctgcgca aggccatccc gtcagcctgc acgttcctgg cgctgtgcgc
     3961 gggggtgggc agcatgttgg cgtccggaca gggaaaattc ctgctggcgg cgcagttgat
     4021 tttggtgtcg ctgattctgg acgggctgga tggcacgctg gcgcggctgc tgcgctcgca
     4081 aacgagcttt ggcgcggagt tggacacgtt tgtggacatt accagcttcg gtctcgcccc
     4141 ggcggtgctg ctctattttt atccgggcgg cctgcacacg ctgccggggt ggggcctggt
     4201 ggtgacgttc ctctacgcga tgtccggcgc ggcccggttg gcgcgcttcc gcgtggtgga
     4261 cccctaccgc ggccagcgcg gtttcctcgg tctgcccatc accacggcgg cgggcttcac
     4321 ggccgcgtat ttgatcgcgc tggaaagccc agcggcgacc cggttgtcgt ggctttcgct
     4381 gcgcgaaggc ccgctggcca tcggtttctg ggcggcaatc ctgctgatgc tggtgttgca
     4441 ggtttcgcat atccgctatt ccaagcccac caagaatccg cgcgtgctga tgccgttcat
     4501 ggtgtgcgtg gtgatgttgt tcattgagcc ggtggccatt tattcggcgg gggcgatgtt
     4561 cgcctatggc gtgtggttcg cgttcatttc gccgttcttt ttccggcgtt ttctggcgca
     4621 tctgccgcca ccggatgaga tggtgcccgt ggctgaagcc gagcacgctg aggaggatct
     4681 gccatgagct ccgcggtcta tgccggaaca tttgatccgc tgaccttcgg ccacctggat
     4741 gtggccgcgc gcgccgcgca ggtgtttagc gaattggtcg tggccgtggc cgaagacccg
     4801 cgcaaaacgc tgctgttcag cgcggaggag cgcatggagt tggcccgggc ctcgctggcg
     4861 catctgccca acgtgaaggt ggagctgttc ggcggcctgc tggtggattg ggcgcgccgg
     4921 cgcggcattc gcaccctgat ccgcggcctg cgcgcgttct ccgactttga atatgaattc
     4981 caaatggcgc tgaccaaccg caagctggcg ccggatatcg aaaccctgtt tttgatgccc
     5041 aacgaagact tcagctatat cagctccagc atggtgcgcg agattgcctc gctggggggc
     5101 agcgtggctc aattcgtgcc ggatgttgtc gccacggcct tgcaccggaa acttacgccc
     5161 aaatgatgcc ctgcggccgg cttgcgggcc ggctccgctc acaggaactt gttcatgatt
     5221 cgccttgcca catcccaaat cggcctcgac ggctaccgcg ttgatggtga agagtctggc
     5281 gagctgctgg acctggcgaa cgatcccgtg gcgcgcgcgg cgggggccat acagtatgac
     5341 ctgaccgtga cccgtgccgg cgacgaactc gtggtacagg gcaccgtggc cgcgccactc
     5401 tcgttgcgct gctcccgctg cgcgcaattt ttctcgacaa ccgtccgcgt ttcgtcattc
     5461 ttgcacgctt atgaatcggc caagcatccg gaatttctgg atgtttccga tgacgtgcgc
     5521 gaggatttgt tgctggaaat ccccggattt cccctctgcc gcgatggctg ccgtgggcta
     5581 tgcgcccaat gcggccagga tttgaacgag agcccgtgcg ggtgcgcgcc tccggagccg
     5641 gaggcgtcgc cgtgggcggt gttggaccaa tggaaggcag cccccggctc gccgccgccc
     5701 cgcggccgcg ccagccaggg gcggacgtga tcaaggagaa agaaaatggc tgaaccaaaa
     5761 cggaaagtat cgaagagccg cgtgcggatg cgccgggcgg cctgggctgg caagattccc
     5821 aaagccgcca cgcaagagtg cccggaatgc ggcgcgccgc ggcaacccca ccgggtttgc
     5881 ccgtcgtgcg gcaagtacaa aggccgccaa gtcatcgtca aggccacgaa ggactaattt
     5941 ccgcgcgccc gccccgcggg cccgtcggga cacgccatgc gtattgctgt ggatgccatg
     6001 ggtggcgatt atgcccccca agccatcgtg gaaggcgccc tgcttgccgt ccgggagtgc
     6061 ccggacatca agtgtctgta tctggtgggc gatgaacaag ccatcgcggc ggaaatcgcc
     6121 cgctgcggcg gcgcgcccga ccgggtgcaa gtgcgccacg ccagcgaggt gatcggcatg
     6181 ggcgattcgc ctgcgaccgc cgtgcgccgc aaaaaggatt cctccatcaa ccgcgccatg
     6241 gacctcgtca aaaacggcga ggccgacgcg gtatttgccg ccggcaacac cggcgcggcc
     6301 gtggcctccg ccacgctcaa gatgcgcacg ctggagggca tccgccggcc cggcattgcc
     6361 gtggccatgc ccacccccgc cgaaaagccc gtgctgctga ttgacggcgg cgccacgccc
     6421 gactgcacac cggaaatcct gatgcaattc gccatcatgg gcacggtcta ttcgcaaaaa
     6481 gtgctgcgcc aagccacgcc aaaagtcggc ctaatgagcg tgggcaccga ggaggccaag
     6541 ggtaacgcgc tcagcaagga aacttttgag ctgctggagt gcgcgcccat ccacttcatg
     6601 ggtaatgtgg aaggccacga cctgtttgaa ggcgtggtgg atgtggtggt gtgcgacggc
     6661 ttcaccggca acgtgatcct caaaaccagc gaaagtgttg cccacgccat caagaaatgg
     6721 ctgcgccgcg agttggtgcg caatccgttg cgctggcttg gcacgctgct gctgtggggc
     6781 ggattgcgct cgctgcggcg caagaccagc cagaaaacct atggcggcgc cccgctgctg
     6841 ggcgtgaacg gcatttgcat catcggccac ggcagctccg ataaaatcgc ggccaaaaac
     6901 ggcatcctgc aagcggtgca ggccgtgaaa ctgcgcgtga acgaggccat cgtggccggc
     6961 cttcagaaat gcgcgcccgc ctccgaagga accccggcat gacaaaaaca atccatgcgg
     7021 ccatcaccgg cgtgggccac tatgcgcccg ccaaggtgtt gaccaacgcc gacctcgaaa
     7081 aaatggtgga taccagcgat gaatggatcc gcacccgcac cggcatccgg gagcggcgca
     7141 ttgccgccaa tgatgaaatg acttccgaca tgggcgtggc cgccgcgcgc gccgcgctgg
     7201 cccaagccaa gttggacccc gccgatgtcg aggccatcta tgtggccacc tgcacgccgg
     7261 acatgatttt tcccagcacc gcctgcctca ttcaggccgc gctgggcgcc aagcgcgcct
     7321 atgggtttga tatttcctcc gcctgcgccg gattcatcat ggcgctggac accgccgccg
     7381 cagccgtgga atccggccgc gtgcgcaacg cgctggtcat tggcgcagaa aaattgagcg
     7441 tggtgaccga ctggaccagc cgcgatacct gtgtgctgtt tggcgatggc gccggcgcgg
     7501 tggtcttgca gccttccccg cggccggggc tgcgttccag tttgctcggc gtggatggca
     7561 accaggccga aatcctgtat attcccgccg gcggttgcaa acagccgtct tccgaagaaa
     7621 ccgtgcgcgc gcaccggcac gccatccaca tgtccggccg cgagacgttc aagattgcgg
     7681 tcaacaccat gcaggaggcc gcacaagagg ccatcgcccg tgccggcctc accgccgacc
     7741 agattgactg catgattccg catcaagcca actaccgcat cgtggacgca gtggccaagc
     7801 ggctcggccc gaatgtgatg gacaaggttt tcctgaatct cgaccgctac ggcaatacct
     7861 cggcggcttc gatcccaatt gcgctttcgg aggccgtcgc tgccggcaag gtgaagagcg
     7921 gcgacaagat tctgatggtg gcttttggcg ggggattatc gtggggcggg gcggtggtgg
     7981 agtggttatg agcacgatgc ttttgttccc cggtcaaggc gcccaagccg tcggcatggg
     8041 ccaggatctc gcggccggtt tgccggaatg ccgcgctttg tttacgcagg ccagcgaggt
     8101 actgggctac gatttgctga agctgtgcgc cgaaggcccg attgaagagc tgaccaagtc
     8161 gcatcaggcc cagccggcca tttttgtggt gagccaagcg gcgcgcatcg cgttggcgca
     8221 cgcgttgggc ggcaagttgg ctgtggcggg tacggcggga catagcttgg gcgagtgggc
     8281 ggcgctgcac gaggccggcg tcatttcatt tgccgatgcc gtgcgggttc tacgcgcgcg
     8341 tggccaattc atgcaggagg cctgcgaggc cacccccggc ggaatgttga ccctgattgg
     8401 gctgccgttg gacaaggtgc aggaagtcgc gcaagccagc ggcctcgaag tggccaacct
     8461 caactcgccc gtgcaaacgg tgctttccgg ccacgcggac cgcatcgccg cgggcgaagc
     8521 ggccgccaag gccgccggcg cgaaacgcgc gctgcgcttg accgtggccg gcgccttcca
     8581 ttcctccctg atgcagccgg cagccgatca actcgctgcg tttttgcaag atgtggaatt
     8641 tcgcgcgccg cgttgccccg tgtggtccaa cgtgaccggg aagccgcata ccacgcccga
     8701 cgaaatccgc cgcctgatgg tcgcccaagt ggtgtcctcc gtgcgctgga ccgatgccta
     8761 tgccgacatg gccgccgccg ggatgaaggt cggctacgag tgcgggccgg gcaccgtgct
     8821 tgccggattg ggcaagcgca tcgtcccgga agtggccatc gccagcattt ttgacttggc
     8881 gggggcgcaa gaggccgcca aactggcggt ctgatggtaa ggaatgggtt gaaatgaaga
     8941 ttttggagaa caaggtggcc atcgtgaccg gcgcggcgcg cgggatcggg caggccattg
     9001 ccctgcaact ggctgccgat ggcgccgatc tggcgctggg cgatgtcaaa gtggaatggc
     9061 tggcggaaac cgccgaaaag gtccgggcgc tcggccgccg cgcggaatgc tacgccatgg
     9121 atgtggccaa tagcgccgtg gtcaacgagg tggtgaccca aatggccgcc gatttcgggc
     9181 acctggatgt gctggtcaat aacgcgggca tcacccgcga cacgctgctg atccgcatga
     9241 ccgaggaaga ctgggacgcc gtgctggata tcaacctcaa gggtgcattc ctggtcacca
     9301 aggccgtggc caagatcatg atgaagcagc gttccggctc gattatcaac atcgccagcg
     9361 tggtgggcgt gatgggcaac ccgggccagg tgaactactc cgcctccaag gccgggctga
     9421 tcgccatgac ccgtaccctg gccaaggaac tcggcagccg caacgtgcgc ctcaatgccg
     9481 tggcccccgg cttcatccgc accgccatga ctgacaaact gtccgagcag gccaaggaat
     9541 acatgctgcg caatgtgccc ctcggccgcc ccggcgagcc cgaggatgtc gccaaggccg
     9601 tggccttcct cgccggcgac aacgccagct acatcagcgg ccagacgctg ccggtttgcg
     9661 gcggcatgat ttggtgatat aagaaaaaga aaacccccaa cggagacata cacatggcac
     9721 tggaagataa agtcagagat atcattgtgg agcagcttgg cgtgaacaag gaacaggtcg
     9781 tgccgacggc ttccttcatt gaagatttgg gcgcggactc cctcgacacc gtcgagctgg
     9841 tcatggcgtt tgaagaagaa ttcggcgcgt ccattccgga cgaggatgcc gaaaagctga
     9901 cctcggtcgg caaggtgatc gaatacctca aggaaaaggg cttcgataag taaatcgtcg
     9961 ccagacgatt ggccgaaggg gccgggactg cgcgccgcaa ggcgacggct cggcccgttt
    10021 ttccaaggat aacccagcag gagaatggac ggtaaacaca tggcacagca aagacgcgta
    10081 gtggtcacag gcttgggcgt ggtcagcccg gtgggcagca cggtggactc gttctgggcc
    10141 gccattctgg ccggccaatc cggcattggg cccatcacct atttcgatgc ttcggcattc
    10201 gacaccaagt ttgccggcca ggtcaaaggc ctgaatctgg acgacttcat tcccaaaaaa
    10261 gaacagcgtc ggatggaccc cttctgccat tacggcgttt ccgccgccaa aatggccatg
    10321 gccgactccg gcctagacat ggccaaggaa gaccccaccc gcatcggcgt catcgctgcg
    10381 tccggtgtcg gcggtctcca gattttacag gcgcagatgg atattctgcg ctcccgcggt
    10441 ccggggcgtt tttcgccgtt catgatcccg caaatgatca ccaacatcct ccccggcctc
    10501 atttccatca atcacggcct caaggggccc aactttgcgg tcgtcaccgc gtgtgcgtcc
    10561 ggcactcatt gcattggcga ggcgctcaat ttgatccgct gcggcgcggc cgatgcgttt
    10621 gtggccggcg gtgcggaagg cgccatctgt gaactcggcg tgggcggctt caacgccatg
    10681 cgcgcgctca gcacccgcaa cgacagcccc accaccgcca gccgtccgtt cgataaggat
    10741 cgcgacggct tcgtgatggg cgagggcgcc gggatgctgg tgattgagga atacgagcac
    10801 gccaaggccc gcggcgccag gatttattgc gaactggccg gcttcggctg caccggcgat
    10861 gctttccata tcaccgcccc cgatgaaagc gcctccggcc cttcccgtgg catgatgctc
    10921 gccatgcagg atgccggcgt caccaccgcc gaaattgact acatcaacgc ccacggcacc
    10981 agcaccgaac tgaacgatgc cggcgaaacc aaggccatca agattgcgct gggcgaggcc
    11041 gatgcccgcc gcacggccat cagctccacc aagagcatga ccggccacct gcttggcgcg
    11101 gctggcggcg tggaatccgt ggcctgctgc ctggccatcc gcgacagcgt gctgcccccc
    11161 accatcaact acaccacgcc cgatcctgcc tgcgacctcg actacgttcc caacgaggcc
    11221 cgccccggca aaatccgcgc gtgcctcaac aactcgctcg gcttcggcgg ccacaacgcc
    11281 accctctgct tcaaggccat ctagtcaacg cccgcctgtt ccgcaaaagc gcggcttgtg
    11341 cgtgacggcg gccggttgct aggattgacc acgaggtatt accgtggcca ccgccaagca
    11401 aaatacgaag cgtcgccaag agcaaattgc ccaagccacc ttggcgcttg tggctgaaca
    11461 tggtttggcg gcgtgcaatg tggccgcagt ggcccgccgc gtggggctgg tgccctccgc
    11521 gctgtaccgg cattttcgca acaaggatca tatgctggat gccgcggtgg aggtcattca
    11581 gagcaaactg ctggataatg tgcgggcggt ttgcgcgcgc caggcccacg ccattgagcg
    11641 cctccacgat ttgctgcaac aacatgtgca attgatcaaa aaaagccggg gcatcccgca
    11701 ggtgatcttc tcgccggact tttatgtgga ccgcccccaa cggcgcgctc gcgtttgtga
    11761 cggcatccgt caatatttgg cggcggtggc ggatatgatt cgggaagggc aggccaccgg
    11821 aactatccgg cccggtctgg atgccgaaac ggccgccgtg ctcttcctgg gcttggtgca
    11881 gccttccgcc gtgctctgga atttgagctc cggcaaattc aatgtggccc gccatgcgga
    11941 aaaagcctgg cccctgttcg tccaaatcct cacaaccggt tgacccatcc gcggcctctt
    12001 cagcgccgca cgaaaaactt caccgtgccc tcttgacaag caaagggccg cagcataatg
    12061 tgaacgtata ttcacatcag gcgggcgtgg actccccgcg ccggccgaat tggagagcaa
    12121 cagcatgaaa aagaaagttc gccttatttt gattgtcctg gtggtgttgg tgggtgccgg
    12181 ggtggcgtgg cggctgacca gccagcgcaa ccggcacacg gatcgcatcc gggtctcggg
    12241 caatatggag gtgtcggaag tggaactgag cttccggatt cccggttggg tggcggcgcg
    12301 cgtcgtggat gagggcatgg ccgtcaacgc cggtgatgag gtcgcgcggc tggatgccac
    12361 cgagctgacg cacgaagtcg aacttcggaa gcacgaagtg gcggcggcgc aggctttcct
    12421 tgcggaactg gaagcgggtt cgcggccgga agaaatcgcg gaggcccagg cgctggccgc
    12481 gcaagcccaa gcccggctgg cggaactcga agccggttcg cgtccgcagg aaattgccgc
    12541 cgcgcaggcg gcggtggcgc gcgcccaagc ggatgccgcc aatcggcagg gcgacctgaa
    12601 ccgcctgacc cagttgcatc aaagcggttc ggtgaccgat caggaactcg attctgccca
    12661 gacggcctcc gccatggctg ccgcccggct gcaggaggcg caggaacaac tccgtttggt
    12721 agaggagggc ccccgtgtgg agcaaatcga acaagcgcgc gccggtttgg cgcaggcgca
    12781 ggagcgtttg gctctggtgc aaaaggggcc ccggcaggaa accatcgcgc aagcccgcgc
    12841 ccgaatggag caggccgaac aaacactggc gatcgcccaa acgcggctgg cgtatgccac
    12901 gctccagtcc cccgtcagcg ggctggtgct cgcagagaat atcgaagctg gcgaatttgt
    12961 gaccgccggc acgcccatcg tgaccgttgc cgacctttcc cgcatctggt tgcgtgccta
    13021 tattaatgag cccgatctgg ggcgagtcaa ggtggggcag cccgtacgcg tgttcaccga
    13081 cacctatccg ggcaaggcgt atcccgggcg gatcacgttc atcgcctcgg ccgctgagtt
    13141 cacgcccaag aatgtgcaaa cctcccaaga gcgcgtcaaa ttggtgtacc gcgtcaaaat
    13201 cacggtggac aatccggagc tggagctcaa gccgggaatg ccggcggatg cggatatcct
    13261 gttgaacggc ggcgaatagg ctgggtgtcg catggatgcg attcgcgtag agcatttggc
    13321 caaatccttt ggcgacctcc acgccgtccg ggatttgtcc ttcgcggttg ccccgggaga
    13381 aatctttggc ttggtcgggc cggatggcgc cggcaaaacc accaccatgc gcctgctgac
    13441 ttccatcatg gagcccaccg ccggcgcggc ctgggtggcg gggcaccaca ttgtggacga
    13501 agcggagcgc atcaaggaaa agatcggcta catgagccag cggtttggcc tctatccgga
    13561 tttgagcgtc gaagaaaaca tccattttta tgcggagatt tatggcgtgt cccgccgcgg
    13621 acgtgccgag aaaatggagc gcctgctggc gttcagcaat ttaaccccgt tcaaaaagcg
    13681 tctggcgggc aatctctccg gtggcatgaa acaaaagctg gggctggtgt gcgccctgat
    13741 tcataccccg caggtgctgt ttctcgacga gcccaccaac ggggtggacc cggtgagccg
    13801 ccgcgacttt tggcgcatcc tctatcagct cttgcgcgac caagtgacca tttttgtgtc
    13861 cacggcctat ttggatgaag ccgagcgctg caaccgcctc gcactgttgg accacggccg
    13921 catgattggc ttgggcaccc ccgacgaaat caagcggctg atgcgcggcg ccattttgga
    13981 ggtgcgcgcc gcaaacccgc gccgcgcctg cgaagtgctg cgcgccgagc tgggcaccgc
    14041 ggccgtcggg ctgtttggcg accgggtgca cgtcgtcacc cgcaaccccg ccgacatgcc
    14101 ccaacagatt cggcacaccc tgcaacaggc cggcctgacg gcaaccggca tccggccggt
    14161 ggacccgtcg ttggaggatg tatttgtttc cgttctggcc gcgcgcgatg aggacaccgc
    14221 atcatgaccc tttccgcccc caaacccgcg gttgtcgtgc atgagctgga gcgccgtttt
    14281 ggccgctttg tggcggtcaa caaggtcagc ttcaccgtga atcaaggtga aatttacggg
    14341 tttttgggac ctaatggcgc cggcaaaagc accaccatcc gcatgttgac cggcattttg
    14401 gcgccctccg gcggcagcgg cactgtcgcc ggtttcgaca tccgccggga aaccgaaaaa
    14461 atcaaaaccc aaatcggcta catgagccag aagttttcgc tctacgacga tttgacggtc
    14521 gaagaaaaca ttgattttta cagcggtatt tatcgcatcc ccgccgccaa gaaatccgcg
    14581 cgcaaacagt gggccctgca aatggcgggg ctgacggagc accggcactc gctggccgcc
    14641 gtgttgtccg gcggctggaa gcagcgcttg gcattgggct gcgcggtgct gcacgagcca
    14701 ccgattctat ttctcgatga accgaccagc ggcgtggacc ccgccagccg ccggcagttc
    14761 tggaatctga tctacgagct ggccgggcag ggcgtgacca tcttcgtgac gacgcactac
    14821 atggatgaag cggaatactg cgaccgcatc ggcttgatct atcgcgggga gttgattgcg
    14881 gagggcacgc cggaacagct taaaaacgaa tggatgcagg aagacgtgct ggacctccag
    14941 tgcgatcggc cgcaggacgc catggcgctc attgaaaccc ttccgggagt caaggaagtg
    15001 gcgcttttcg gcaaggggct gcatgtggtg gccacgcaca gcgaaactgc cctggccgag
    15061 ctgaaaacgg cattgccccc ggccggcttt gccatccaac ggctggaggc catcacgcct
    15121 tccctcgaag acgtgtttgt ttccctgatc gaagcgcgcg accgggcgga tgccgattca
    15181 cgggaggttc ggcgatgatc ccgggcaagt ggttggtgtt taaacgcata tatgctgtgg
    15241 cccgcaagga aatgctgcac gtggtccgcg acccgcgcag cctcggcatg gccattgcca
    15301 tccccatgct gctgctggtg ctctttggct acgcattgac gctggatgtg gacaacgtgc
    15361 cgctggtggt ctgggatcag gatgaaaccc ccgccagccg cgagctggtc agccgctttc
    15421 tcggctcgca ctacttttcg ctcggcgaat acgcccgcga ttaccgccag gtggagcggg
    15481 cggtggatac gggcaagtcg ctggcggggc tggtgattcc tcgcggcttt gccggcggca
    15541 ttgccgccgg ccgcgaaacc cccgtgcagt tgctggtgga tggcagcgat tcgaccaccg
    15601 ccaccctggc catgggctat gccgataccg tggtgcgcgc ctactcgcag gatattgtgc
    15661 tgcggcaggc gcaacgaacg ggcggcgtcg cgcggcgcgt gcccctcgat ctgcggccgc
    15721 gggtttggtt caacgcagac atggagtcca agaactacat catccccggc ttgattgccg
    15781 tgatcatgat ggtgattgcc gcgctcctga cctccctgac cgtggcgcgc gaatgggagc
    15841 gcggcaccat ggagcagttg atttccactc ccatcaaggg ctgggagctg gtcaccggca
    15901 aacttctgcc ctacttcctg attggcctgc tcgatgtggc gttggccgtc ctcatgggcg
    15961 agtttctgtt tcaagtcccc ctgcggggca gcgtggcgct cctcttcacg ctggcggccg
    16021 tcttcctggc cggcgcgctg gcgctgggcc tggtcatcag cattgtcacc aaaagccagt
    16081 tgctggcgag ccaactcgcc atggtgctga cgttcctgcc ttccttcctg ctctccgggt
    16141 ttatgtacgc catcagcaat atgccccggc caatccaact cgtcacctac accatcccgg
    16201 cgcgctactt tgtggcgttg ctcaaaggca tctatctcaa gggcgtgggc ttgcgcattc
    16261 tcctgctgga agccggcctg ttgaccatct ttgctgttct cattgtgctg ttggccgtcc
    16321 gtaaattccg gaaaaagctg gggtaaggtt catgttcgag cgaattgtcc acatgttgca
    16381 caaagagttc cggcaaatct tccgggatcc caaaatgacc cgcatcatct ttttgatgcc
    16441 cgtcatccag ttgctggtgt ttggctatgc cgtcaccacc gacgtcaaac acgtgcggac
    16501 cgcggtctgc gacctcgaca actccgtggc gagccgggaa ctggtggctc ggtttgtgca
    16561 ttccggctat ttcaccatca ccgcacgggt cgagacggaa caggaaatac gggaactgat
    16621 ggaccgcggc caagtccagg cggtgttgca aatcaaccac ggctttggcg ccgacgtgca
    16681 ggcgggcaaa accgcgccgc tgcaaatcat cgtggacggc accgattcta ccacggccgg
    16741 cattgtgctg gattatagcg ccaagatcac cggcctctat tcgacccaaa tcatccggca
    16801 tcaaaccgaa cgcctggcgg gtgcggcgcc cacgcccgga acggttgccc tgcaaacccg
    16861 cgcctggttc aacgataatc tcgaaagccg caacttctac gttccgggag tcattgccat
    16921 catcgtgatg ctcattacgc tgatgctcac cagcatggcc gtcgtgcggg aaaaggaaat
    16981 cggcacgatg gagcaaatca tggtcacccc catcacgccc acggaattca tcctgggcaa
    17041 aacggtgccc tttgccctca tcgggtttgc cgatgtggtg ctcgttgccg tggtgggcgt
    17101 cttttggttt gcagtgccca tccgcgggag cctgcccctg ctgttcggcg ccaccgcgtt
    17161 gtacctgatg accacgctgg ggatcggcct gctcatttcc acggtcagcc aaacccagca
    17221 acaggcgatg atgagcacct tcttctttta tttccccgcc gtcctgctca gcggcttcat
    17281 gttccccatt gccaacatgc ccaccgtcat ccaatggctc acctacctca atccgctgcg
    17341 gtattatctc gtggtgattc gcggcatttt cctcaaaggc gtcggcccgg gcattctctg
    17401 gccgcagatg ctggcgctgg gcgtgatggg cgtttgcacc ttgtggctgg cttcccgccg
    17461 cttcaacaaa accatggcgt aaccttacgg agacacacat gaaaaaaaat cgaatcaaac
    17521 cgctctgggt gctggccgcc gcgactgctc tgctcatggc cggcggttgc gccaaccctc
    17581 tgctcactcc cgccgtgccg gacatcgccg attatttggc tgcgtcaccg agcccggaag
    17641 cacccacggc tgacggagat gtcgccgtgg ccggggcgcc gctggaactg gccgcttgcg
    17701 tgcgcatcgc gctggcggat aatccggtgg gccaggccgc ccgcgaaggc gtggccgccg
    17761 cccgggaaaa tatcggtatc gcgcgctcgc gcttctatcc gcaagtgggg ctcaccggca
    17821 gctatcgccg ctgggaatcc cacgcgtttc tgcctgacag cttgagcggc gccatggcca
    17881 gcacccccgc cacggtgggc cccaccgacg actggagcgc gggcggcgtg gcgagtttct
    17941 atctcttcga tagcggccgg cgccgcgccc agctcctcgc cgcgaaagcc cagtgggggg
    18001 cgcagcaagc cgaacaggcc gccgtggagc aagacatcgc cctgaatgtc catcaggcat
    18061 tctacagcct gctggccgcc caggaatcgc tgcgggtggc ccaggaaaac agcgcgcggg
    18121 cggaagatca ttttcggctg acccaggacc gcaaatcagc gggaatggcc gtacaggccg
    18181 atgtcctgcg cgcacaagtc gaagtggcgg accgccacct ggccattgtc cgcgcggagc
    18241 atggggtgcg cgtagcccgc ggcaacctca atttggccat gggactgccc gccgaaatgc
    18301 cggtggaaat tgactccgcc gcgcccggcg cgccggtgga acccgcccca ttggccgatg
    18361 acttggccac cgccatggag cagcgcccgg aactgcgcgc ggcattgaac cgggtcgcca
    18421 gcgcccgcag cggagtttcc gcggccaaga gcgagttcgg tcccgtggtc aaagccgacg
    18481 cccgctatgg ctggcgcgat accgaattta cgccggaaga caaagactgg ctgatcggcg
    18541 tcggcatcga attgcccctg ttcaccggct tttcccgcac tcaccagttg caacgcgccc
    18601 ggcacgaact ctccaaagaa gaagcgctgg tgcggcaact cgccctgacc atccggcagg
    18661 aagtgtggga cgcgcactca cgcgttcagg aggccatgga agccttggat gccgccggaa
    18721 ccctggtcgc cagcgcccgt gaaagccagc gcttgtcccg ccagcggttc gaagccggca
    18781 ccaacaccat caccgacctg ctcgacgccg aagccgccca agcccgcgcc gaaggcctcc
    18841 aagtggaagc gcgctggaac tacttcatca gccgcgccgc gctcgcccgc gccatcggcg
    18901 cgatggacac aaaataaagc cacgggcgcc gtgcctgtca cgcgcgggaa atttacgcct
    18961 cgtggtccag cgggtgcgtg accgtgcatt cgggcctttt tgcgtcgtgc gaagggccat
    19021 cagcttgctc agcggaggta gtttggcgct tggggttggg agtactttcg caacaccggc
    19081 gacgagcctg ccttcggcta tttaacctga accctgaacc ccgaactctg acttcttttc
    19141 ccgatgaaaa tggtggtggg gggtggattc gaaccaccga actcatagag ggcagatttc
    19201 tcgccggcgg cgagctcgcc tatggcgaga cagtctgctg gttcaaccac tcccggccct
    19261 tgccggattt cagataggcc tcccgtttga gggcagccga gaagtcacca gaggtttcga
    19321 tgtgcagcag tttcaggggc cgctgattgt gggttcgctt gccacgaccg gcatgatggt
    19381 cccgtaatcg cgcctgcaag ttccgtgcgg caccggtgta acgcaatccg ttttgcccca
    19441 agaggacgta cactccaggg tctgtgggaa tttggttgag aatctgactc aactcttgca
    19501 tggctgattc ggagatggtg gtgggggatg gattcgaacc atcgaactca tagagggcag
    19561 atttacagt
//
LOCUS       DAOPNP010000038        19295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3464_length_19295_cov_7.907017, whole genome shotgun sequence.
ACCESSION   DAOPNP010000038 DAOPNP010000000
VERSION     DAOPNP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19295
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_19295_cov_7.907017"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKI64_05060"
     CDS             <1..360
                     /locus_tag="PKI64_05060"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE00415.1"
                     /translation="IGGMYRAPHGAVCAALLPHVMAANHRALQARAPESPLAARYDEV
                     ARLLTGNPAARAADGVAWVAQLAADLQIPPLATYGVQPDAIARIVEQAQKSSGMKANP
                     IEMTTDELADILRRALG"
     gene            complement(400..1056)
                     /locus_tag="PKI64_05065"
     CDS             complement(400..1056)
                     /locus_tag="PKI64_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOE00416.1"
                     /translation="MKILKVPYTLGEEIAHAITHGLGALLGIAGLAVLMVAAATRGTG
                     AWQVVPCAIYGAMLILMFTVSTLYHSLPQPRVKKVFRVLDHQMIFLMIAGSYTPFVLI
                     TLRGALGWWLFGVVWGIAALGLVFKAFFTGRFGKITTGLYLLMGWIIVFALKPLLAGM
                     SAGGVRWLVAGGLCYTLGAVVYLFKRVRYHHAVWHLLVLGGSICHYFAILWHVLPPRA
                     "
     gene            complement(1483..2505)
                     /gene="waaF"
                     /locus_tag="PKI64_05070"
     CDS             complement(1483..2505)
                     /gene="waaF"
                     /locus_tag="PKI64_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOE00417.1"
                     /translation="MVTPPAPAPARLLVCSVNWLGDAIMAMPALQALHEKWPKTEVSI
                     LTKPGLAPLWALHEVPNQVFTMPKGTFGLLGLAKPIKNMDFEVSYILPHSFRSALPPF
                     LAKIPQRIGFPGHFPRDFMLTEVRPPAGGAGRIHQVFEYLDLFFPGENRQNYPQPRLT
                     VPETTLTSMRDKLSSLPKPWIAILPGAARGPAKQWPAENYAKTAALLVAQTGGSVITL
                     GTASERAVCQQVAASAAPNGLNLAGSTTLPELAAVLALANVVLCNDSGGMHLAAALGT
                     PLVAIFGITNPQQTGPIGQKIRILQQSDQKTRDIPRKSQAAAAALRSISPAEALQATL
                     DLLASA"
     gene            complement(2499..3941)
                     /locus_tag="PKI64_05075"
     CDS             complement(2499..3941)
                     /locus_tag="PKI64_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00418.1"
                     /translation="MKKSALLALALLAPGIVLAAMPRSIALVIQENGNVQVSETHDIE
                     PAGPDGLVRISPLPETLVATSVNATPADRSDNLDIAAQRFAYDLRNDTTLFHAYVGET
                     ITCHGEDDPVTGRLATLPDFTAPHPALMLEAAGQTVHYIPDIFALDSIEFPSRPALAR
                     QPTLLWQLAAGQMPPPSVQLNYAATGISWSAAHEAILAEDGRSFALASRVRIQNKTSR
                     DFVNARVRLALTEKGLYTPLVPATDDPRAAKPPALRYSADGASWVPERLGASAGIVAT
                     YDLPQALTLPAGGDVRAGLVTAPAVAAETRLVYDGVRFDRYQRNRRNDWNLGTESASI
                     VETRLTFQNTLPTPLPPGEFRLLRGRADKTLDWIGTDWLPALKPGESATINLGPAAGL
                     TGRRLRTAYSDVVPFKVAEETFEITVENQTATAQTVTVIEHFYRGDNHQITAASAEYT
                     PGTAPHSIQFDVPVKPGSSQSLTYTVRYTW"
     gene            complement(3938..5044)
                     /gene="aroC"
                     /locus_tag="PKI64_05080"
     CDS             complement(3938..5044)
                     /gene="aroC"
                     /locus_tag="PKI64_05080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOE00419.1"
                     /translation="MSSTFGTLFRVTTFGESHGRAIGCIIDGCPARIPLTETDIQPQL
                     DRRRPGSWALTTPRKESDTVTILSGTDRGLTLGTPIGLTIPNADTKPLDYSAMEAVPR
                     PSHADFTYAEKYGLRARSGGGRASARETAARVAAGAVAEKMIRTFHPVEIVAWVESIG
                     ANVAPRPDLATITRADVDKIPVRCPDAATANKMIAAIAEAKQAGDSVGGVIACAVRGV
                     PAGWGEPVFEKLTAELAHAMMSIPATRGFEIGEGFGATLMRGSQHNDPFTAKDGGGIR
                     PATNHCGGIQGGISNGEIIFFRVAFKPVSTISLPQPTAAFDGTPIVFEAQGRHDPSVL
                     PRAVPVVEAMTALVLADAFLQQQVQLIPRPGATP"
     gene            5289..6308
                     /gene="hydE"
                     /locus_tag="PKI64_05085"
     CDS             5289..6308
                     /gene="hydE"
                     /locus_tag="PKI64_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOE00420.1"
                     /translation="MADLLPLLLASGEELTRLYARADAVRREYCGEAVPVRAILEFSN
                     YCANECLYCGLRAENAVPTRYRMSADDILAAVDQIAATGVAGTVVLQAGETPGYSDDE
                     ISALLRRIKAAHPALAITLSVGNRPRAVYARWREAGMDRYLLRFETSDPELYARLHPN
                     GSLAERLQCLRDLRAVGVQTGSGFMIGLPGETLPTLARNILLCREFDLDMIGSGPYIP
                     NPDTPLGAKPNAYADDPEMFFRAIAVLRLVNPAAHIPATTAFDAVFPGGRDLVLQRGA
                     NVFMPNATPTSLRRDYQLYPGKPNVDESAAQFADRAVARLRAIGRPVAAGPSHSLKRP
                     PPEQE"
     gene            6324..7199
                     /gene="mutM"
                     /locus_tag="PKI64_05090"
     CDS             6324..7199
                     /gene="mutM"
                     /locus_tag="PKI64_05090"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOE00421.1"
                     /translation="MPELPEVETIVRHLCTARLGGQVIRRASVHWAKTVAPHSPAGFA
                     RQIRNSRIETIARRAKYIVMTLADGRSLIIHLRMTGHLDILPPQTPPRRSDRMILELA
                     DGRQLHFEDARKFGRVWLTRNSDQILSKLGPEPLTLRVADFRRRLHGRTQQLKPLLLD
                     QHFIAGIGNIYADEALWHAQLHPRRRADTLSPQQVAALYEAIRVVLRRGIRNLGTTLG
                     GGTTHFVLPQGERGRNQEKLHAYGQTDLPCTRCGTKIKRLVVNGRATHICPACQRAPR
                     RASRRGRIALRVKTG"
     gene            7210..7509
                     /locus_tag="PKI64_05095"
     CDS             7210..7509
                     /locus_tag="PKI64_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOE00422.1"
                     /translation="MNVQRFIQRICSIAIVALLLAIVALRFYVPKIREEQALKKELAQ
                     KTLDFQREAEELRLLRLKQEKLREDPEFLEKIARENLGYTKPGETLFLFKDEDAP"
     gene            complement(7487..8554)
                     /gene="eboE"
                     /locus_tag="PKI64_05100"
     CDS             complement(7487..8554)
                     /gene="eboE"
                     /locus_tag="PKI64_05100"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HOE00423.1"
                     /translation="MTYCLNVHDGGSWAVVRRALAEQVLAVKQLAGSEGQFPLSLHLG
                     QQALAEAMQPSARRQFRDWMLENHCFLAGLNAFPFGHFHEAHVKEKVYRPNWAQAERL
                     DYSRQALSFLADLMPSDWAGTITTVPGAWQADWTTPVDEEQASANLCRAGEHCRSIFT
                     ETGRKVRVAIEPEPGCAWKLFDPRVEQAANEICWCLDTCHAAVEFQSIEHLPWERIGR
                     VQLSAAIECDNTPAAREALTPFAEPRYLHQTRAALDGEIIGSWPDLPPALAELSKLPA
                     KAIVRTHYHVPLTWSGTGPLRSTRANLTPTFLRQAQRVFWEVETYTYSVFPPHLRPPS
                     LPAAIAAELAWARDAMARLHP"
     gene            complement(8596..9600)
                     /locus_tag="PKI64_05105"
     CDS             complement(8596..9600)
                     /locus_tag="PKI64_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE00424.1"
                     /translation="MARKLRIQFKGAIYHLTIRGIERRNIFRDDHDRLRFLAQLAEAG
                     AESGVRLYLYCLMNNHAHLLAETPRANVSAFMHRLQTAYTVYFNRKHQRVGHLVQGRF
                     SSKLVQGDQYLLNLSRYIHLNPVFAGIMAAKPLKTRRQFLRNYRWSSYRGYAGLSSVE
                     DIVDRTALLSMMPGKGEQQRKAYRTFVESGLATTDEEFRNLVHQSPLAIGNPSFQEQA
                     QQRYAHALRETSRPEDVALRRLAFAHNATSVIQITCEEAGATPQQLTQRSYRQPARAI
                     AMWALGKYAGLSQREIAETLQVKTGAAVCQAIRRLKMSAATDHQLQAVMRQIERRLVQ
                     "
     gene            complement(9669..10184)
                     /locus_tag="PKI64_05110"
     CDS             complement(9669..10184)
                     /locus_tag="PKI64_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00425.1"
                     /translation="MGRPPCHRGGDCPNRRSKRDRADIIAVPTVSAIVKGSNYTEHHS
                     FSGHTADKQPVQVEYTDEIQEWLIQYRDAQPIKGELPSAGQIRAIEEVNVVCPHFKVH
                     KFIVDQTYIVFISIREHGLGLLHSSNQSALPLTSRYSLTWFEATKDPHNPYELKTAEM
                     TQEEYIRWLAK"
     gene            complement(10237..11394)
                     /locus_tag="PKI64_05115"
     CDS             complement(10237..11394)
                     /locus_tag="PKI64_05115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOE00426.1"
                     /translation="MTLHLQKITVRHEFPVVFTRALFSPRNRSLHAALTRDGLSTRRR
                     CLVLVDKGVLTTFPDLTAHIRQYFAAYSTDLHLVRAPLVLPGGERIKQTPVAVRRILA
                     ALHRHNICRHSYCLAVGGGAFLDAAGFAAALFHRGVRHVRIPTTALAQCDSGVGVKNA
                     INYAGTKNLLGTFAPPWAVLNDTAFLDALPQPLLLDGIAEAVKVAAIKDAAFFRAIER
                     AAQRIRQRHLPTIRRILKRCALLHLDHIATSGDPYELGTARPLDYGHWAAHKLEILSR
                     HRLSHGQAVAIGIALDALYASRIGLLPLKQAQRLIATLRTCGLPVWAPELAQTDRRGR
                     LLILSGLEEFRAHLGGQLTITLPAPLGSRREIHAVDTALMASCLLGSALNN"
     gene            complement(11508..13175)
                     /locus_tag="PKI64_05120"
     CDS             complement(11508..13175)
                     /locus_tag="PKI64_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOE00427.1"
                     /translation="MNNSAPAAPETPAKNMHFIREIIAEDNRSGKYGGKVVTRFPPEP
                     NGYLHIGHAKAICLDFGMALENNGVCHLRMDDTNPVKEDVEYTDSIKEDVRWLGFDWG
                     PHYYHASDYFEQMYELACGLIRDGKAYVCELSSEEWKDYRGIPTEPGKESPFRNRSPE
                     ENLDLFQRMRAGEFPDGAKVLRAKIDMASPNLHLRDPVIYRILHTPHPHAGDKWCIYP
                     MYDYAHPIEDAIEQITHSMCTLEFEVHRPLYDWVIQNCRLFPSQQIEFARLALTYTVM
                     SKRKLLELVQQGLVNGWDDPRMPTLSGLRRRGVPPTAIREFCERVGITKYESLTDVAL
                     LEHCIRDDLNKTARRAMAVLDPLRVVIENAGEIPTAIRGINNPEDPAAGEREIPFGAE
                     LFIEREDFALVPPPKYFRLSPGKAVRLRNAGFLTCTGVEQDAAGNVTAVRGRFDPMDA
                     PIKVKATIHWVPAHAATPVEVRLYDRLFNVPEPDADKNVDFKTHLNPNSLQVVQALVE
                     PSLLAVQPGDRFQFERIGYFYAEPDSRPGAPVFNRTVTLKDSWKSGA"
     gene            complement(13172..14362)
                     /gene="dnaJ"
                     /locus_tag="PKI64_05125"
     CDS             complement(13172..14362)
                     /gene="dnaJ"
                     /locus_tag="PKI64_05125"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOE00428.1"
                     /translation="MSQKRDCYDVLGVARDATENHIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEATEAYEILADAERRQMYDQYGWAAFERGNGRGFGGMGHMDIEDALRAFASAF
                     GGGMGGGFFEGLFGGMGGSRSRSERSRGADLRYDLEIAFEEAAFGAKRELTLDIVEDC
                     GTCHGTGAAAGSKRETCTQCGGRGTVVTSNGFFQMRQPCPRCGGTGEYVRNPCPACRG
                     QGRAKVSRRIELTIPAGVDTGSRLRLAGKGEGGARGGPPGDLFVVLHVRPHDLFARDG
                     LDTHCEVPIPFHIATLGGEVQVPTLRGDVPLKIPAGTQPGKVFTLRGLGLPDVRGRST
                     GDHHVAITIEIPTDLARNTSDLLAAFGASVKETNHPRLAALRRKAKQFYSHKNAMNPT
                     PGDS"
     gene            complement(14381..14953)
                     /locus_tag="PKI64_05130"
     CDS             complement(14381..14953)
                     /locus_tag="PKI64_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE00429.1"
                     /translation="MTKKHAHSPETPAKPAAEEKPVPPAEPVEEPTPAPPVEPTVEPE
                     VEPPEPLQHQLLRLQADFDNFRKRIARERKDWIAAANEDLVTELLPVLDHFELGLADG
                     AKNGVPPAMLEGFQLIYNQLMGVLSKAGVEPIDAVGKLFDPHWHEAMTHQPSDTVPAE
                     HVVAQTRRGYKMGDKLLRAAQVLVSSGPAA"
     gene            complement(14997..16307)
                     /locus_tag="PKI64_05135"
     CDS             complement(14997..16307)
                     /locus_tag="PKI64_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00430.1"
                     /translation="MPIPAAGRLFISNSELMMLVLNPIQPSLAEAEVRWLKRYGWLFP
                     VLGFPLGLLIKGALWSGTLLNASFLPSNLAGWLSPLGLLIGTIISLVPVAWWLMLTLE
                     IKIKSGGRGWLLAPVMACVCFILLEMAFRTYPVQAIFWQGVIARAGDQTQAREVGLYR
                     LQAAADRKAKSPAGFVFPGSSQVVQGIDCAELSQLTGAPVYRRAVSGLFPIELICSQG
                     LITFNHDNRLILLLSGFDVGARADLVPDAIRPQATWSGLRHVLRAAPASFQLRHWRNF
                     VDLCFAASCDLWRSRDYVRFMWENPVAPNIIRNNAAAQSALNQQLQGYQRLGSNPEMV
                     ALCWQALDLFLGDMAQHFQNIIVFESRINPTSPPSASALDLENQTRRFFENQQHLQRI
                     RFIPRAAQHLDLPPEYWADNTHVNAAGRQLLTEMIARFVDDTSP"
     gene            complement(16264..17526)
                     /locus_tag="PKI64_05140"
     CDS             complement(16264..17526)
                     /locus_tag="PKI64_05140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOE00431.1"
                     /translation="MIALSTTVDFVASNGIYRSDDPQTRKRLLVFSIVINLGILGFFK
                     YFNFFVDSATALLAGFGLETPRLNLHIILPVGISFYTFQSMSYTLDVYRGQLVPTRRF
                     TDYALFVSLFTQLVAGPIERAKNLLSQVTSPRVVTWSGIQTGTWLFFWGLFKKVVIGD
                     NLALLVDQVFGGSVAWNTGSVLLGIYAFALQIYCDFSAYSDMARGLGMYLGFNIMINF
                     RNPYFALNPSELWRRWHISLSTWLRDYLYIPLGGNRRGPRRTYINLLLTMVLGGLWHG
                     AAWTFVLWGAFHGGLLALHRWFQERWPPAPTLSWAGRGWRTLAMFHATCFGWLLFRAN
                     SMRDVTGMLKALLQPGWLPEFPGWIFQILLLALPLLGIQILQERTQDPLAPLRLSLFP
                     RIILYTFMLFMLVTYANTGSRAFIYFQF"
     gene            complement(17694..18857)
                     /locus_tag="PKI64_05145"
     CDS             complement(17694..18857)
                     /locus_tag="PKI64_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00432.1"
                     /translation="MPPKKKSAPDAPVAQGDARIFLVYGSDDLSATRKADELVARLCP
                     PEEQAFGLETFSPESDQDSADAVCTVLRNVMEALLTAPFLGGNKTVFLRQAPYFDPLS
                     EPGRFASVKAEVERLVNLLKKGLPAGVSFVLLTRKVNKATTFFKTFQSRGEVHAFDEP
                     EKDSEVKADYIPLVKKLVAERQLTMEDGVLGAFLDRAGYNLRQAANELDKLDLYLGDR
                     RTVTREDIELMVAPVRAGKFWEFAETFCSGQLDKTLQTMHRLLAQGEEPLALLIMLQN
                     RLRDIVVMADCLKRGWLQVSGGDWPRLTWSVPPEGEELLSPLGKRDPRKGNPYAMGKM
                     AAQAKRLPPSRWFRWLNAAVDAQAAMTGGDNVPPATTLEVFTTRTLGELTAPK"
     gene            complement(18890..>19295)
                     /locus_tag="PKI64_05150"
     CDS             complement(18890..>19295)
                     /locus_tag="PKI64_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00433.1"
                     /translation="PRSKLAGLLLPAEELRACIARRARQMFAHGLVEEVRELRARFPE
                     LSPTARHAIGYAEAADVLAGLISEEEAIRRTTLRTTQYAKRQMTWFRRQADVAWIPVG
                     PRDDTARLAGKVRAIWATHGPAPLRPHRAKTN"
BASE COUNT         4066 a         5333 c         6090 g         3806 t
ORIGIN      
        1 attggcggga tgtaccgtgc gccgcacggc gcggtttgcg cggcgctatt gccgcacgtc
       61 atggcggcca accatcgcgc attgcaggcc cgcgcgccgg agagcccgct ggccgcccgc
      121 tatgacgagg tggcgcgact gctgaccggc aaccccgcgg cgcgcgcggc ggacggcgtg
      181 gcgtgggtgg cgcaactcgc ggcggattta caaataccgc cgctggcaac gtacggggtg
      241 cagccggatg caattgcgcg aattgtcgag caggcgcaaa agtcatccgg catgaaggcg
      301 aatccgattg agatgaccac tgacgaattg gcggatattc tgcggcgcgc actgggttga
      361 gccgtcggcg ttcgcgcgct gcgcaacgcg gtgcgggcat cacgcccgcg ggggaaggac
      421 gtgccagagg atggcgaaat agtggcagat gctgccgccc agcaccagca agtgccagac
      481 ggcatggtga tagcgcaccc gtttgaacag atagactacc gcgccgagcg tgtagcacag
      541 gccgcccgcc accagccagc gcacgccacc ggcggacata ccggccagga gaggtttcag
      601 cgcaaacacg atgatccagc ccatcagcag gtaaagcccg gtggttattt tgccaaaacg
      661 ccccgtgaaa aaggccttga acaccagccc cagcgctgcg atcccccaca ccacgccaaa
      721 cagccaccaa cccaacgcgc cgcgcagcgt aatcaacaca aaaggcgtgt aactgccggc
      781 aatcatcagg aaaatcatct ggtggtccag cacccggaag acttttttca cgcgtggctg
      841 cggcaagctg tggtagagcg tcgaaaccgt gaacatcaaa atgagcatcg cgccgtagat
      901 ggcgcaggga accacttgcc atgcccccgt gccgcgcgtg gcggcggcca ccatcagcac
      961 tgccagcccg gcaatgccca acaaagcgcc caacccgtgg gtaatggcgt gggcgatctc
     1021 ttcgcccaac gtataaggaa cttttaggat tttcattccc cccatgatac tcctctttta
     1081 cccggcgcgg ggatttattt gttggtcgga ggcaaatgat gggctttcgg gttcttcgta
     1141 ttaagatgct tcgagccgaa atgaacaagg gatctgatgg cggcaaagct caaattcaga
     1201 ttctcctaaa attcaacaaa gggccgaaat aatcaaaatt gttagataaa aacgtttgat
     1261 ttcacttata attataagtg aaatcaaagg ccacaaaata cgttgtaacg catttggatg
     1321 tagcggatta tatattattt atgttcttcc accaattttg ccgttgtttg cgaggctttt
     1381 gtcgggccag aatggttgag ggaagaaacc ctcccccccc gtgaaaaggt ataaggcctt
     1441 cgactattcc atctctaaga ctaatcagcc ttattttagc aattaagccg aagcaagcag
     1501 gtccagagtg gcctgcaagg cttctgcggg ggaaatggag cgcaaggcgg ctgcggcggc
     1561 ctgcgattta cggggtatat cgcgcgtttt ttggtcggac tgctgcaaaa ttcgtatttt
     1621 ttggccaatt gggcccgttt gttgagggtt tgtgatgcca aaaatggcta ccagaggggt
     1681 tcccaaggct gcggcaaggt gcattccgcc gctatcgttg cacaaaacca catttgccag
     1741 tgccaaaacg gcggccagct cggggagagt ggtgcttccg gccagattta gcccatttgg
     1801 ggcggcggaa gccgccactt gctggcagac ggcgcgttcg ctggccgttc cgagggtaat
     1861 tacgctcccg ccagtctggg ccacgagtaa agcggcggtt ttggcgtagt tttccgcggg
     1921 ccattgtttg gccgggcccc gggcggcacc gggtaaaatg gcgatccaag gcttgggcag
     1981 gctcgatagt ttgtcgcgca tggacgtcag cgtggtttct ggcaccgtca agcgcggttg
     2041 cgggtagttt tgacggtttt cgcccgggaa aaagaggtcc aaatactcaa aaacctgatg
     2101 aatgcgcccc gcgccgccgg caggggggcg gacttcagtc agcataaaat cacgcggaaa
     2161 atggcccgga aagccgattc tttggggtat tttggccaaa aaaggtggca aagcagaccg
     2221 aaaagagtgg ggaaggatgt atgaaacctc aaaatccatg tttttgatgg gttttgccaa
     2281 gcccaacaaa ccaaaagtgc cttttggcat agtaaaaacc tgatttggca cctcgtgcag
     2341 ggcccaaagg ggggccaaac ccggtttggt gaggatggaa acttcggttt tgggccattt
     2401 ttcgtgcaac gcttgcaggg cgggcatggc catgatggca tcgcccagcc agttgaccga
     2461 gcaaaccaga agccgggccg gggctggggc ggggggggtt accatgtgta gcggacggtg
     2521 taggtcaaag actggctgct tcccggcttg acgggcacgt caaactggat ggagtgcggg
     2581 gccgtaccgg gggtgtattc cgcgctggcg gccgtgattt gatggttgtc gccgcggtaa
     2641 aaatgctcaa tgaccgtgac ggtttgcgcg gtggcggttt ggttttccac cgtgatttca
     2701 aaggtttctt cggccacttt gaagggaacc acgtccgaat aggccgtgcg caagcgccgc
     2761 ccagtgaggc cggcggcggg acccagattg atggtggccg attcaccggg cttgagtgcg
     2821 gggagccaat cggtgccgat ccaatcgagt gttttgtctg cgcggccgcg caacaggcgg
     2881 aattcaccgg gggggagggg ggtggggagg gtgttttgga aggtcaggcg cgtttccacg
     2941 atggaggcgg actccgtgcc gaggttccaa tcgttgcggc ggttgcgttg atagcggtca
     3001 aaccgcacac cgtcatagac caagcgtgtt tcggcggcaa ccgccggtgc ggtgaccagg
     3061 ccggcgcgga cgtcgccacc ggccgggaga gtcagcgcct gcgggaggtc gtaggtggcc
     3121 acaatgccgg cggaagcgcc aaggcgctcc ggcacccaac tggcgccatc tgcgctatag
     3181 cgcagtgcgg gcggcttggc cgcgcggggg tcatcggtgg cgggcacaag gggggtgtag
     3241 agcccttttt ccgtcagcgc cagacggacg cgggcgttga cgaagtcgcg gctggttttg
     3301 ttttggatgc gcacacgcga ggcgagggca aaagagcgac cgtcttcggc caaaatggct
     3361 tcatgggcgg ccgaccagga aatgccggtg gcagcgtagt tgagttgcac ggaggggggg
     3421 ggcatctggc cggcggcgag ttgccaaagc agcgtgggtt ggcgcgccag tgcggggcgc
     3481 gagggaaatt caatggaatc gagcgcaaaa atgtcgggga tgtagtggac ggtctggccg
     3541 gctgcttcca gcatcaaggc cggatggggg gcggtaaagt cgggcagtgt ggcgaggcgg
     3601 ccagtgacgg gatcgtcttc gccgtggcag gtgatggttt cgccgacata ggcatggaaa
     3661 agggttgtgt cattgcgcag gtcatacgca aaacgctgcg cggcgatatc cagattgtcg
     3721 gaacggtcgg cgggagtggc gttgaccgat gtggcgacca gcgtctcggg cagtgggcta
     3781 atccgcacca ggccgtcagg gccggcaggt tcgatatcgt gagtttccga aacctgcaca
     3841 ttgccgtttt cctgaatgac cagtgcgatg gaacgcggca tggcggccaa cacaatgcct
     3901 ggcgccagca gcgccaaagc caagagggcg gatttcttca tggggtggct ccggggcgcg
     3961 ggatcaactg gacttgctgt tgcaggaatg cgtcggcgag aaccagggcg gtcatggctt
     4021 ccaccacggg cacagcccgc ggcaggacgc tgggatcgtg ccggccctgc gcctcaaaca
     4081 caatgggtgt gccgtcgaaa gcggcggtgg gttgcggcag cgagatggtg gatacgggct
     4141 tgaaggccac gcggaagaaa ataatttcac cgttggaaat accgccttgg atgccgccgc
     4201 aatgattggt ggccgggcgg attccgccgc cgtctttcgc ggtgaaggga tcgttatgtt
     4261 gcgaaccgcg cattagtgtg gcgccgaagc cttcgccgat ttcaaacccg cgcgtagcgg
     4321 ggattgacat catggcgtgg gccagttctg ccgtcaattt ttcgaaaacc ggctcgcccc
     4381 agccggcggg aacaccgcga accgcgcaag caatcacgcc gccgacagaa tcgccggctt
     4441 gtttggcctc ggcgatggcc gcaatcattt tgttggcggt ggccgcgtcc gggcagcgca
     4501 cggggatttt atccacatcg gcgcgcgtaa tggtggccaa gtcggggcgg ggggccacgt
     4561 tggcgccgat ggattccacc cacgccacaa tttccaccgg atggaaagtg cgtatcattt
     4621 tttccgccac ggcgccggcg gccacgcggg ccgcggtttc gcgggccgag gcgcggcccc
     4681 cgcccgaacg ggcgcgcagg ccatattttt cggcataggt gaagtccgcg tgcgacggcc
     4741 ggggcacggc ttccatggcg gagtagtcca gcggtttcgt gtcggcgttg gggatggtca
     4801 gcccgatggg cgtgccgagc gtgagcccgc ggtctgtgcc ggaaaggatc gtgacggtgt
     4861 ccgactcctt gcgcggggtg gtcagcgccc aggagcccgg tcgccgccgg tccagttgcg
     4921 gctggatgtc ggtttcggtc agcggaatcc gcgcggggca gccgtcaatg atgcacccga
     4981 tggcgcggcc atgggactcg ccaaacgtgg tgactcgaaa caacgtgccg aaggtactgg
     5041 acatatcaat ctcctcgtcg ggaaagagta ggcacagccg cgcccggtgg gaagcgaaaa
     5101 gtatgagcga ttgacgcttc ccggcaacgg acgcgggcgc atttttgccg tcgtattttg
     5161 cccgcccgga cgaatccgcg ccggcggttt gacgcggcct tgcggccaaa gctttcggtt
     5221 gccgggtgag tgtgatagtt ttccacgcta tggaaaatat gcttccacag tgtggaaaaa
     5281 atccgggcgt ggctgatctg ctgccactgc tgctggcatc gggcgaggag ttgacccggt
     5341 tgtatgcccg ggcggatgcg gtgcgccgcg agtattgcgg agaggccgtg ccggtgcgcg
     5401 ccatcttgga gttttccaac tactgcgcca atgaatgcct ctattgcggc ttgcgggcag
     5461 agaatgcggt tcccacccgc tatcggatga gcgcggatga tatcctcgcg gccgttgacc
     5521 agatcgcggc gaccggcgtg gcggggacgg tcgtgttgca ggcgggcgaa acgccgggat
     5581 attccgatga tgaaatcagc gccctgctgc ggcgcatcaa ggccgctcat cccgcgctgg
     5641 ccattacgct ctcggtggga aatcgcccgc gcgcggtgta cgcgcgttgg cgcgaggcgg
     5701 gcatggatcg ctacttgctg cgctttgaaa cctcggatcc ggagttatat gcccggttgc
     5761 atccaaacgg ctcgctggcc gaacgcttgc agtgcttgcg cgatctgcgc gcggttgggg
     5821 tgcagacggg cagtggattc atgatcggcc tgccgggcga aaccctgccc acgctggccc
     5881 ggaatatttt actgtgccgg gaattcgatt tggacatgat cgggagcgga ccctatatcc
     5941 ctaatcccga tacgccgctg ggcgcaaagc ccaatgccta tgccgatgat ccggaaatgt
     6001 ttttccgtgc gattgcggtg ttgcggctgg tgaaccccgc cgcgcacatt cccgccacca
     6061 ccgcgtttga tgccgtgttc cccggtggcc gggacttggt tttgcagcgc ggggccaatg
     6121 tgtttatgcc gaatgccaca ccgacatctt tgcggcgcga ttaccaactc tatcccggca
     6181 agccgaatgt ggacgaatcc gccgcccagt ttgccgaccg tgccgttgcg cggttgcgcg
     6241 ccatagggcg gcccgtggct gccggcccca gccactccct gaagcggcca ccgccggaac
     6301 aagaatagcc gccttgcacc gccatgcccg aattaccaga agttgaaacc atcgtgcgcc
     6361 acttgtgcac ggcccgcttg ggcgggcaag ttatccggcg ggcgagtgtt cactgggcca
     6421 aaactgtggc cccgcacagc cccgccggtt ttgcgcgaca aatccgcaac agccggattg
     6481 agacgattgc tcgacgggcc aagtatatcg tcatgacgct ggccgatggc cgctctttga
     6541 tcattcattt gcgcatgacc gggcatctgg atattctgcc tccgcaaacg ccgccgcgtc
     6601 gctcagaccg catgatattg gaattagccg atggtcggca attgcacttt gaagatgcac
     6661 gcaagttcgg gcgggtgtgg ctcacaagaa attcagacca aattctgagc aaactggggc
     6721 cggagccgct tacgctccgc gtggccgatt ttcggcgacg gctgcacggg cgcacgcagc
     6781 agctcaagcc tctgttgttg gatcaacact tcattgccgg tatcggcaac atctatgccg
     6841 atgaagcgct ttggcacgca caactccatc cgcgccgccg ggcggataca ctatcgccac
     6901 agcaagtggc ggcgttgtac gaagcgattc gtgtggtact gcgccggggc atccgcaacc
     6961 tcggcacgac gctgggcggg ggcactacac attttgtttt gccccaaggg gagcgcggac
     7021 gaaatcagga aaaattgcat gcctacgggc aaaccgattt gccttgcact cgctgcggca
     7081 cgaaaatcaa gcggctggtg gtcaatggcc gggccacaca tatttgcccc gcctgccaac
     7141 gggccccgcg ccgggccagc cgccgcggcc gtattgcctt gcgcgtgaaa accggctagg
     7201 atgccaacga tgaacgtcca aagattcatt caacgaatct gctcaatcgc gattgtggcc
     7261 ctgctattgg caatcgtggc cctgcggttt tatgtgccca agatccgcga agagcaggcg
     7321 ctcaaaaagg agttggccca aaaaacgttg gattttcaac gcgaagccga agaactgcgc
     7381 ctgctgcggt tgaagcagga aaagttgcgg gaagaccccg agtttctgga aaagattgct
     7441 cgtgagaacc taggctatac caagcccggc gaaaccctgt tcttgttcaa ggatgaagac
     7501 gcgccatagc gtcgcgcgcc caagccaact cggcggcaat ggccgccggt aacgaggggg
     7561 ggcgcagatg gggtggaaaa acggagtagg tataagtctc cacttcccag aatacgcgtt
     7621 gggcttgccg caggaatgtg ggggtgaggt tagcgcgcgt ggagcgcaag gggccggtgc
     7681 cggaccacgt cagcggcaca tgataatgcg tgcgcacgat ggccttcgcc ggcaatttgg
     7741 atagctccgc gagggcgggg gggaggtccg gccatgagcc gataatttca ccatccagcg
     7801 ccgcgcgagt ctggtgcagg tagcgcggtt cggcaaatgg ggttagggcc tcgcgggcgg
     7861 ctggcgtgtt gtcgcactca atagccgctg acaactgcac ccgcccgatt cgttcccaag
     7921 gcagatgttc aatggattga aattccacag ccgcgtgaca ggtatccaaa caccaacaaa
     7981 tctcattagc ggcttgctcc acacgcggat cgaacaactt ccacgcgcaa cccggttccg
     8041 gctcaatggc cacccgcacc ttgcggccgg tttccgtaaa aatggaacga caatgctcac
     8101 cagcccggca caggttggcc gacgcctgct cctcatccac cggcgtggtc caatccgctt
     8161 gccatgctcc cggtacggtg gtgatggttc ccgcccaatc cgacggcatc aaatcggcca
     8221 gaaagctcaa tgcttgccgg gagtaatcca agcgctctgc ttgcgcccaa ttggggcggt
     8281 acaccttttc tttgacgtgg gcttcatgga aatgtccaaa ggggaacgca ttcaatccgg
     8341 ccaggaaaca atgattctcc aacatccaat cgcgaaattg ccgacgggct gatggttgca
     8401 tcgcctccgc cagcgcttgc tgccccaaat gcaaactgag cgggaactgg ccctcactcc
     8461 cagccaactg tttgacggcc aaaacttgct ccgccagtgc ccgccgcacc accgcccaac
     8521 tccccccatc atgaacattc aagcaatacg tcatccgggg tcagccctta acaattaaca
     8581 atgttctccg ccctgctact gaaccaagcg acgctcaatt tgccgcatca cggcttgcaa
     8641 ttggtgatcc gttgccgcgc tcatttttag ccggcggata gcctggcaaa ccgctgcgcc
     8701 cgttttgact tgcagcgttt cagcaatttc tcgctggctt aacccggcgt acttaccgag
     8761 cgcccacatc gcgatggctc gcgccggttg gcggtaggag cgctgggtca gttgttgggg
     8821 ggtagctccc gcttcctcac aggttatttg aataaccgat gtggcgttat gcgcgaaggc
     8881 aagtcttcgc aaggcgacat cttcaggacg actcgtctcc ctgagcgcat gggcgtaacg
     8941 ctgttgtgcc tgttcttgaa atgagggatt gcctatggcg agcggggatt ggtggactag
     9001 attcctgaat tcttcatctg ttgtggctag gcccgattca acgaatgtgc gataggcttt
     9061 gcgctgttgt tctcccttgc cgggcatcat gctcaacaga gcagtgcgat caactatgtc
     9121 ttccacggaa ctaaggccgg catagccacg ataactgctc caacggtaat tgcgcagaaa
     9181 ttggcggcgg gtcttgagtg gtttagccgc catgatgccg gcaaatactg ggttcaaatg
     9241 aatgtaacgg ctcaaattta acaggtactg atcgccttga accaacttgg aagagaagcg
     9301 gccctgtact aaatgtccca cacgctggtg tttgcgatta aagtataccg tataggcggt
     9361 ttggagccgg tgcatgaagg cgctaacatt ggcacgtgga gtttccgcga gcaggtgagc
     9421 gtgattattc attagacagt acaagtaaag ccgcacccca ctttctgccc ctgcttctgc
     9481 caactgcgcg agaaaacgca ggcgatcatg gtcatcgcgg aagatattcc ggcgttcaat
     9541 gccgcggatc gtcaaatgat agatcgcacc tttgaattga atccgtagtt tacgtgccat
     9601 ggttgaagtt tggcagaagc gccccgacaa tgtcaattgt taagggctga ccccaagagt
     9661 aaaatgcgtt atttggctag ccaacgaata tactcctcct gggtcatttc cgcggttttg
     9721 agctcatagg ggttgtgcgg atctttggtt gcttcaaacc aagttaagga ataacggctg
     9781 gtaagaggca gtgccgattg gttgctgctg tgcagcagtc ccaagccgtg ttcccggata
     9841 ctgatgaaca caatgtatgt ttgatcaacg ataaatttgt gaactttaaa atgtgggcag
     9901 acgacattta cttcctcaat agctcgtatt tgtcctgcgg aaggaagttc gccttttata
     9961 ggctgggcgt cgcgatattg gatcagccat tcttgtatct catctgtata ttcaacttgg
    10021 accggttgtt tatcggcagt gtgcccagaa aatgagtgat gttcagtata gttggatccc
    10081 ttgactatgg cgctgacagt tggcacggcg atgatgtcag cacggtcacg ttttgatcgt
    10141 cggttagggc aatctcctcc gcggtggcaa ggtgggcgac ccacaacgcg aagcctgcca
    10201 gaacaatata tttacgtttc ataatggtcc agattgttaa ttgttaaggg ctgaccccaa
    10261 gagacaggag gccatgaggg cggtatccac ggcgtggatt tcgcggcggg agccgagggg
    10321 ggcggggagg gtgatggtga gttggccgcc gagatgagca cggaactctt ccaaaccgct
    10381 caagatcagc aggcggccac ggcggtcggt ttgcgcgagc tcgggggccc atactggcag
    10441 gccgcacgtg cgcaaagtgg caatgagccg ctgggcttgt ttgagcggca agaggccaat
    10501 tcgtgaagcg tagagcgcat ccagcgcaat gccaattgcc acggcctggc catgcgaaag
    10561 gcggtgccgg gagaggattt ccagtttatg cgccgcccag tggccataat ccaaagggcg
    10621 ggcagtcccg agttcgtacg gatcgcccga ggtggcaatg tggtccagat gcaacagggc
    10681 gcagcgtttt aaaatccgcc ggatggtggg caggtggcgt tgtcggattc gctgtgccgc
    10741 gcgttcgatc gcgcggaaaa aggcggcatc tttgatcgca gcgaccttga cggcttccgc
    10801 gatgccatcg agcaaaagcg gttgggggag ggcatcgaga aaggcggtat cgttcaatac
    10861 ggcccacggc ggcgcaaagg tgcccaacag atttttggtg ccggcataat tgatggcatt
    10921 tttaacgccc actccggagt cgcattgggc gagtgcggtg gtgggaatgc gcacgtggcg
    10981 cacaccacga tggaagagcg ccgcggcgaa gccggccgca tcgagaaaag cccctccgcc
    11041 gactgcgagg caatacgaat ggcggcaaat gttatgccgg tgcagcgcgg ccagaatgcg
    11101 ccggacggca actggcgttt gcttgatgcg ttcgccgccg ggaagcacca gtggtgcgcg
    11161 gaccaaatgc aaatcggtcg aatacgcggc aaagtattgg cggatgtggg ccgtcaaatc
    11221 cggaaatgtg gtcagcacgc ctttatccac cagcaccaga caacggcggc gtgtcgaaag
    11281 tccatcacgg gtaagcgcgg catgcagcga ccgattccgt ggggagaaca aagcgcgtgt
    11341 gaacacgacc gggaattcgt gtcggactgt aattttctgc agatgaagcg tcacagcggt
    11401 atcctgcacc ggggttcagc ctaccggaac cacagccaaa ccatcaacat gcaagcgccg
    11461 gataagggcg cggggttcaa cacggcaccc cttttgatgg cgagagttca ggcaccggac
    11521 ttccaagaat ccttgagcgt gacggttcgg ttgaataccg gggccccggg gcgactgtcg
    11581 ggttcggcat aaaagtagcc gatacgctcg aattggaagc gatcaccggg ctggacggcc
    11641 aacaggctgg gttccacaag ggcttggacc acctgcaatg aatttgggtt gagatgggtt
    11701 ttgaaatcca cgtttttgtc ggcgtcgggc tcgggcacgt tgaacaggcg gtcgtagagc
    11761 cgcacttcga cgggtgtggc cgcgtgggca ggcacccaat gaatggtggc tttgactttg
    11821 atgggggcat ccatcggatc aaaacgcccg cgcaccgcgg taacgttgcc ggcagcgtct
    11881 tgttccacgc cggtgcaagt gaggaaaccc gcattccgca ggcgcacggc cttgcccgga
    11941 ctcaaacgaa aatacttggg cggggggacg agcgcaaagt cttcgcgttc gatgaataat
    12001 tccgcgccaa aaggaatttc gcgttcgccg gcggcagggt cttccggatt gttgatcccg
    12061 cgaatggcag tcggaatttc gccggcattt tcaatgacaa cccgcagcgg atcaagaacg
    12121 gccattgcgc ggcgcgcggt tttgttgagg tcgtcgcgga tgcaatgttc gagcagcgca
    12181 acatcggtca gcgattcata tttagtgatg cccacccgtt cgcagaactc gcggatggcg
    12241 gttggcggga ccccccggcg gcgcaggccg ctcagcgtcg gcatgcgcgg atcatcccag
    12301 ccgttgacga gaccttgttg caccaactcg agcagtttgc gcttgctcat gacggtgtag
    12361 gtcagggcta ggcgggcaaa ctcgatttgc tgcgaaggga aaaggcggca gttttggatg
    12421 acccaatcat agagggggcg atggacttca aactccagcg tgcacatgga gtgggtgatt
    12481 tgctcgatgg catcctcgat ggggtgggcg tagtcgtaca tgggataaat gcaccacttg
    12541 tcaccggcgt gggggtgggg ggtatggagg atgcgataaa tgaccgggtc acgcaggtgc
    12601 aggttgggcg atgccatgtc aattttggcg cgcaacacct tggcgccgtc gggaaattca
    12661 ccggcgcgca ttcgttgaaa cagatcgaga ttttcttcgg gcgaacggtt gcgaaatggt
    12721 gattctttgc ccggttcggt tggaatgccg cggtaatcct tccactcctc ggacgatagt
    12781 tcgcaaacat aagctttgcc gtcgcgaatc aggccgcagg ccagctcata catttgctca
    12841 aaataatcgg acgcatgata atagtgtggg ccccaatcaa aacccagcca ccggacgtct
    12901 tccttgatgg aatccgtata ttcaacatct tctttgacgg gattggtgtc atccatgcgc
    12961 aggtggcaga ccccgttgtt ttccagtgcc atcccaaaat cgaggcaaat ggccttggcg
    13021 tggccgatgt gcagatagcc attgggttcg ggggggaagc gcgtgaccac cttgccgcca
    13081 tatttgccgg aacggttgtc ttcagcgatg atctcacgga taaagtgcat attcttggcc
    13141 ggtgtttccg gcgcggcggg ggcggagttg ttcatgagtc acctggcgtg gggttcatgg
    13201 cattcttgtg gctataaaac tgtttggctt tccgacggag cgcggccagc cgcggatgat
    13261 tggtttcctt gacggatgcc ccaaacgccg cgagcaggtc gctggtgttg cgcgcgagat
    13321 cggtggggat ttcgatggta atggccacat ggtgatcgcc ggtggagcgg ccacggacat
    13381 ccggcaggcc aaggccgcgc aacgtgaaca ccttgcccgg ttgggtgccg gcaggaatct
    13441 taagcggcac atcaccgcgc agcgtgggca cctgcacttc gccgccgagc gtcgcaatgt
    13501 ggaaggggat gggcacttcg caatgagtgt ccaatccgtc gcgtgcgaac aagtcatgcg
    13561 ggcgcacgtg caggacgaca aacaaatcgc cgggcgggcc gccgcgcgcg ccaccctcgc
    13621 ctttaccggc caagcgcagg cgcgaaccgg tatccacgcc ggcgggaatg gttagttcaa
    13681 tgcgccgcga gactttggca cggccttggc cacggcaagc cgggcaggga ttccgcacat
    13741 actcgccggt gccgccgcaa cggggacacg gctggcgcat ctgaaagaag ccgttggaag
    13801 tgaccaccgt gccgcgcccg ccgcactggg tacacgtttc gcgtttgctg cccgcggccg
    13861 cgccagttcc atggcacgtg ccgcagtcct cgacgatatc caatgtcaat tcccgcttgg
    13921 cgccaaaagc ggcttcttca aacgcgattt cgagatcgta gcgcaggtcg gccccgcggg
    13981 agcgctcgga gcggctgcgg gagccaccca tgccgccaaa cagtccttca aaaaaaccgc
    14041 cacccatgcc gccgccaaaa gccgaggcaa acgcgcgcag cgcatcctcg atatccatgt
    14101 gccccatgcc gccaaagccg cggccattgc cgcgttcgaa cgccgcccag ccgtactggt
    14161 catacatctg ccgccgctca gcatcggcaa gaatttcata ggcctcggtg gcctccttga
    14221 atttttcttc cgcttccttg tcgcccggat tgcggtccgg gtgatatttc atggccagtt
    14281 tgcgataggc cttcttgatg tggttttcgg tggcatcgcg ggccacacca aggacatcat
    14341 aacaatcgcg tttctggctc atggcgactt tcgggcgcgg ctaggcggcc gggccggatg
    14401 acaccagcac ctgcgccgcc cgcagcagtt tatcgcccat tttgtagccc cggcgggttt
    14461 gggccaccac gtgttctgcc ggcacggtgt ccgacggctg gtgcgtcatc gcctcgtgcc
    14521 agtgcggatc gaaaagtttg ccgacagcat caatcggttc caccccagcc ttggaaagca
    14581 cgcccatgag ctggttataa atcaattgaa aaccttccag catggcgggg ggcacgccgt
    14641 ttttggcgcc atcggccaag cccaattcaa agtggtccaa aaccggcaga agttcggtca
    14701 caaggtcttc gttggcggcg gcgatccagt cttttcgctc gcgcgcaata cgtttgcgga
    14761 agttgtcaaa atcggcttgc aagcgtagca actgatgctg aagaggctcc gggggctcaa
    14821 cctccggttc caccgttggc tccaccgggg gggcgggggt gggctcctcc actggttctg
    14881 ccggcggaac aggcttctcc tccgcggcgg gcttggccgg agtttcaggc gaatgggcgt
    14941 gtttttttgt catatcaggg tcggaacgat acgcagcccg cgcgggttcg tcaaccctac
    15001 ggggatgtgt cgtcaacgaa ccgagcaatc atttccgtta aaagctgccg cccggcggcg
    15061 ttcacatgcg tattgtcggc ccagtactcc ggggggagat cgagatgttg agcggcccgc
    15121 ggaatgaagc gaatccgttg gagatgctgt tgattctcaa aaaatcggcg ggtttggttt
    15181 tccaaatcca gcgcactcgc cgacggcggg gaggttggat ttatccggct ttcaaacacg
    15241 ataatgtttt gaaaatgctg tgccatgtcg cccaaaaaca agtcgagtgc ttgccagcag
    15301 agagcaacca tttcggggtt ggaccccagg cgttgatacc cttggagctg ttggttgagg
    15361 gcgctttgag cggcggcatt atttctgatg atattgggcg ccacagggtt ttcccacatg
    15421 aaccgaacat agtcgcgcga acgccaaaga tcgcacgagg cggcaaagca caaatccacg
    15481 aaattgcgcc agtgccgaag ctggaaagaa gcgggcgccg cacggagcac atggcgcaaa
    15541 ccagaccacg tggcttgcgg gcgaatggca tccggaacaa gatccgcacg cgcacccaca
    15601 tcaaagccag acagcaataa aatgagccgg ttgtcatgat tgaaagtgat caacccctga
    15661 ctgcagatca gttcgatggg aaacaaccca gacaccgctc gccgatacac tggcgctccg
    15721 gtcaactgcg acaactcagc gcaatcaatc ccttgcacaa cttggctgga gccggggaaa
    15781 acgaagccgg cggggctttt cgccttgcgg tcagcagccg cctgcaaacg atacaggccc
    15841 acttcgcgcg cctgcgtctg gtctccggca cgcgcaatca ccccctgcca aaatatggcc
    15901 tgaaccggat aggtgcgaaa agccatttcc agcagtatga aacagacgca ggccatgact
    15961 ggcgccagca gccaaccgcg accgccagat ttgattttta tttccagcgt gagcatgagc
    16021 caccaggcca ccggtaccaa tgaaatgatt gttccaatga gcaagcccag cggagacagc
    16081 cagccggcca gatttgaggg caaaaacgaa gcatttagca gggtcccgct ccacaaggcc
    16141 cccttgatga gcaggcccag cgggaagcca agcaccggaa agagccaccc ataccgcttc
    16201 aaccagcgta cttcagcttc ggccagagag ggttggattg gattcaaaac cagcatcatc
    16261 agctcagaat tggaaataaa taaacgcccg gctgccggta ttggcatagg tcacaagcat
    16321 gaataacata aatgtataga ggattatgcg gggaaaaagg ctgagacgta gcggggccag
    16381 cggatcttgc gtgcgctctt ggagaatttg aataccaagc aggggaaggg ccaagagtaa
    16441 aatctggaaa atccagccgg ggaattcggg aagccaaccc ggttgcagca atgctttgag
    16501 catgccggtc acatcacgca tggaattggc ccggaacaac agccaaccga aacaagtggc
    16561 atgaaacatg gccagcgtac gccagccacg acccgcccag gaaagggttg gagcgggcgg
    16621 ccagcgttct tggaaccagc gatgcagggc caggagcccg ccgtgaaagg caccccatag
    16681 cacgaaagtc cacgccgccc cgtgccacag gccacccaac accatggtca acagcagatt
    16741 gatgtaggtc cgccgcggtc cccggcggtt cccgcccagc ggaatataca agtagtcgcg
    16801 caaccaagtg gataagctga tgtgccaccg ccgccataat tcgcttggat tcagcgcaaa
    16861 atacgggtta cggaagttga tcatgatatt gaatcccaaa tacatgccca agccccgggc
    16921 catgtcgcta tacgcggaga aatcgcaata aatctgaagt gcgaaggcgt aaatacccag
    16981 cagcaccgac ccggtattcc aagcgaccga accgccaaaa acctgatcca cgagaagggc
    17041 gagattgtcg ccgatgacta cttttttgaa cagaccccag aaaaacaacc aagtgccggt
    17101 ttgtatgccg gaccaggtga caacgcgtgg cgaggtcact tgcgacagca ggttttttgc
    17161 gcgctcgatc gggccggcaa ccagttgtgt aaaaagggac acaaacagcg cgtaatcggt
    17221 aaaccgccgg gttggcacca gttgcccgcg ataaacatcc aaagtgtaac tcatggattg
    17281 aaaagtataa aaggatatcc ccaccggcag gatgatgtgc aggttcaggc gcggagtctc
    17341 caggccaaat ccagccaata gagctgtcgc gctatccaca aaaaaattga aatacttgaa
    17401 gaaccccaaa attcccagat tgatcacaat actgaaaacc agcagtcgtt ttcgtgtttg
    17461 cggatcatcg gaacggtaaa tcccgttgga ggccacgaaa tccacagttg tggagagcgc
    17521 gatcaaggaa aggaaacgcc aatcccagca cccgtaaaaa aaataactgg ccacgagcag
    17581 caggatattt tgtccgcggt gcggcagcca aacgtatgcc gtaaacacca cgacaaaaaa
    17641 gatgatgaaa tccaacgaat tgaacagcat ggcggaggat ggagacggtc aggctacttg
    17701 ggggctgtca gttcgccgag ggtgcgggtg gtgaagactt caagagtggt ggcggggggg
    17761 acattgtcac cgccggtcat ggcggcttgg gcatccacgg cggcattcag ccagcggaac
    17821 cagcgggatg gggggaggcg cttggcttgc gcagccattt tgcccatggc atagggattg
    17881 cccttgcgcg ggtcgcgttt gccaagaggg gacagcagtt cttctccttc gggcgggacg
    17941 ctccacgtca ggcgcggcca atcgccgccg gagacttgca gccagccgcg tttgaggcag
    18001 tcggccatga cgacgatatc gcgcagccgg ttttggagca tgatgagcag ggcaaggggt
    18061 tcctcgcctt gcgccagcag gcggtgcatg gtttggaggg ttttgtcgag ctggccggag
    18121 caaaaggttt cggcaaattc ccaaaacttg ccggcgcgca cgggggcgac catcaattca
    18181 atatcttccc gggtgacggt gcgacggtcg ccaagataca ggtccaactt gtcgagctcg
    18241 ttggcggctt ggcgcaggtt atagccggcg cgatccaaaa atgcgcccag cacgccgtcc
    18301 tccatggtga gctggcgttc cgccaccaac tttttgacta gcggaatgta gtcggctttg
    18361 acctcggaat ctttttcggg ctcgtcgaac gcatggactt cgccgcgcga ttggaacgtc
    18421 ttgaaaaaag tggtggcctt gttgactttg cgggtgagca aaacaaacga cacacctgcg
    18481 gggaggcctt ttttgagcag attcacgagg cgttcgactt cggccttcac gctggcgaag
    18541 cggccgggtt cgctcaacgg atcaaaatat ggcgcctggc ggagaaagac agttttgtta
    18601 ccgccgagaa atggggcggt gagcagtgcc tccatcacat tgcgcagcac ggtgcagacg
    18661 gcatcggctg aatcctgatc ggattcgggg ctgaacgttt ccagtccaaa cgcctgttct
    18721 tcgggcgggc ataggcgggc caccagctca tccgccttgc gcgtagcgga gagatcatcg
    18781 ctgccataca ccaaaaagat tcgcgcgtcg ccttgggcaa cgggcgcgtc gggggcggat
    18841 tttttcttgg gcggcatgag ggggagcgtc cgacatctcc cgcttttttt caatttgttt
    18901 ttgcgcggtg ggggcggagg ggggcggggc cgtgggtggc ccaaatggcg cggactttgc
    18961 cggcgaggcg ggcggtatcg tcgcgcgggc cgacgggaat ccaagccacg tcggcctggc
    19021 ggcggaacca cgtcatttgc cgtttggcat attgcgtggt gcgcagggtg gtgcggcgga
    19081 tggcctcttc ttcggagatc aagccggcga ggacatcggc ggcctcggcg tagccgatgg
    19141 cgtggcgcgc ggtgggcgac aactccggga agcgcgcgcg cagttcgcga acttcttcaa
    19201 caaggccatg ggcaaacatt tggcgggcgc ggcgggcgat gcacgcgcgg agttcttcgg
    19261 cgggtagcag caggccggcc agtttggagc gcggg
//
LOCUS       DAOPNP010000039        18054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3730_length_18054_cov_8.776043, whole genome shotgun sequence.
ACCESSION   DAOPNP010000039 DAOPNP010000000
VERSION     DAOPNP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_18054_cov_8.776043"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..115)
                     /locus_tag="PKI64_05155"
     tRNA            complement(22..115)
                     /locus_tag="PKI64_05155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Ser,seq:gct)
     gene            369..3182
                     /locus_tag="PKI64_05160"
     CDS             369..3182
                     /locus_tag="PKI64_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE00434.1"
                     /translation="MPQSAELRPILGNEPTAFLRYEVPAAVTNVQISGSWDMWKLPQP
                     MTRDAAGMWVFDVRDLQARLGRYEFKFIVDGAWESGANRVLPINLEGIIERPPAMIQR
                     AAIDDFQLIRVFFKQSLPAGIQPQATLDPPVPVAWTEVVTAEEDARRSGYLLSRGMVT
                     FIFDQAAYGLELARTTQVVVAGNFTGWDGGGGGGKWVLKRGRLAGTWELAAQLGGMRL
                     PPGEKELVFKFVLNGTEWLTPPEDAPNALADGKGSINLKLDILRSGGAEIRVHTAEPL
                     DLRQSYILKLDGVTERTLGVMTTPDGVLDRLYSDKTMGVTLDKVRGTTTYRLFAPRAK
                     NAWLCFFDRPLAVSWTPTFHRFPPAEEFRMWQDPADGVWEITTRGLDVGRFYGFRVDG
                     PTGDGESFDPAAVVGDPYGRAAANADGLTLVMDPDATNQWFRGWTDQAWETPDPQDVV
                     IYECHVRGMTVHPSAGIPAAKRGKFAGLAASLGTGTGLDHLKTLGVNAVELLPVSEFS
                     EGADLYNWGYATVYFFAPESSYAQEPEKGSGYYELKQLVNDLHNQGFAVILDMVYNHV
                     GGPNIFETLDKKYYFRLNTDLGHSNNSACGNDVKTEAPMMRKLILDNIEYFMREFHVD
                     GFRLDLAELVDMETMLAIRDRARAINPKAILISEPWSPGRGENKYQLRGTGWSAWNND
                     FRYAAKDFVRGQGNREWLKGSVFGSVDLWALNPLQPVNYLESHDDIAYIDELTANPGR
                     DGRQLTDRDAALNRLAATILFTSLGKAMLYEGQEFLRSKWGINNTYNRGDAVNAVRWT
                     DRDRPLARQALDYYAGLIQLRMSPEGAAFRVAQRPPRTYYRWLLPDNPKLMGYVVNVP
                     ELHAGRGFAVLLNADTRERTMDVELGGSTAWKMIGNGREINPRGLTPAGWPAGKSPPS
                     WPAGWRGLIEVPKQEAIILMNGF"
     gene            3194..3937
                     /locus_tag="PKI64_05165"
     CDS             3194..3937
                     /locus_tag="PKI64_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOE00435.1"
                     /translation="MDFLQQLVARLPAEPVPVRGVWIGPYWTAVQTARGTGLATTTLA
                     DGPTHGPHAQHMAAAGTLVGRDARELAAGVASSFALARSLGLAALNALLPEPIGKISE
                     RNAGELACAMGAGQVVGVVGWFPFIPQLKKHAAAVEIFEKDPQTGYTLTTERAERLAR
                     CDGLCVTASALVNQTLGGILAAVQPGAWKILVGPSAPLCAESLELGFTAICGARVTDT
                     DAVVRVIQEGGCFQQIRQTGGVRLLTLET"
     gene            complement(3966..5294)
                     /locus_tag="PKI64_05170"
     CDS             complement(3966..5294)
                     /locus_tag="PKI64_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HOE00436.1"
                     /translation="MKRLVFASLLIAATMANAVAPADFGITRNLFPVYNETDHLAELR
                     LDAAVCALTQPGFTDVRLFTQPGGQDTPYVLEAIRSTREQTRRINLSPAKADLRVVSD
                     QITTAFIELPHDAPPAQGLEVRTPQQNFVRNVRISGSEDNQFWRPLAEAAIFDFSQFI
                     NMRRTEIPLPANSCRFFSIEIVEAGPDAVAQLAALLRDNKRDPAPANELLKMPFHTTG
                     INLWNDSTTLVPDALELQEWPPVSVTIAQDKRQKITEIIATTLGAPITRFEFDIPPGN
                     FSRIVTVQVSAVRDGKPTWRTVGDGILSSTLLPDYATNQLTVTFPEQRSPQMRLVVQN
                     ANSAPLNIAGVRAYGPTYRLFWISTARSNYTLAYHNDQVAAPAFDDSAIRAALAAGEP
                     ADLWHLHPAALPEYKAPRPILPLLQRPSTFIVLAGIVLLVLGALYARMRK"
     gene            5431..6585
                     /locus_tag="PKI64_05175"
     CDS             5431..6585
                     /locus_tag="PKI64_05175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOE00437.1"
                     /translation="MIPTWAVVGGGWLVVWLALAVGGRGPLWPPELPAQPVGGDLAMM
                     LGYAQSWLATGDPYAALNPYPPLAAVLFAPLTKLPFAAALAVMTALTLFVFVVTTAWL
                     PRLVWPRAERKAVWLVALADLFSYGLFFELRWGQFNVVALGCAAWGIFCFHRACGPAM
                     RLVGYALFCVGVQLKLYPALLVFLFTKDARAWGVNLWRWAALGAVNAALLFALGGAVA
                     QAFGHSVQAQAAAPYVWVGNHSLQSWAAGVGQPAVVPWLFAGYALCLGWGLARAWWQP
                     TAIPVANAALLGVLGMLLLPGVSHDYKLSLLPMGFAGVVAAHGAGLVWRRENLGRLLT
                     LAGVSVMVAWTLFPPAAKPAALQNNAPALLAGCILLAWGGGGVSRKDGGK"
     gene            6582..7094
                     /locus_tag="PKI64_05180"
     CDS             6582..7094
                     /locus_tag="PKI64_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE00438.1"
                     /translation="MKLERVTGPSQIAAVAELAREIWTAYYTPLIGAAQVGYMLAAFQ
                     SAEAITRQIGAEGYEYYLAPGEGYLALVPDPAKQSMFLSKIYVKEHLRRTGLGRAMME
                     LAEARCRETGCRELWLTVNRHNAGSIAFYEKMGFRKTNPLVTDIGGGFVMDDWRMAKT
                     IPLAAANPTE"
     gene            7302..8093
                     /locus_tag="PKI64_05185"
     CDS             7302..8093
                     /locus_tag="PKI64_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOE00439.1"
                     /translation="MGAMQELDREAALAVAVAAVKEAGRLLLELRQQPVEVLSEPGHD
                     IKLKADQLAEERILAILHERFPLPVLTEESGEHGAVNESGRMWVVDPLDGTFNYSRGM
                     PLCCSAIGLWENGQPVLGAVYNFFRDELFTGIVGQGAWRNGERVRASGETRVAKASLA
                     TGFPHQQSYEEAPLLAFVRNVQRFKKIRMLGSAALMGAYVSCGWLDGYVEEDVWLWDI
                     AGAVAVAKAAGAVVTVRPGKAGRWAREVVCAASPELHAALAAPAP"
     gene            8121..9491
                     /locus_tag="PKI64_05190"
     CDS             8121..9491
                     /locus_tag="PKI64_05190"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOE00440.1"
                     /translation="MSKLKIEKIHTLVTVDDDYRVLHDVDVVLAGDKIEAVGPNLPTP
                     EGARVLDGRWCVAYPGFVNTHHHFYQTLTRNIPAVQNSKLFDWLLWLYEVWRGLHPEA
                     VRVSTQVALGELLLSGCTTSTDHFYVFPQSAPQEFLDIEIDTAREIGVRFHPTRGSMS
                     LGRSQGGLPPDDVTQSGEAIMADCERLIRKYHDPKPFAMTRIVLAPCSPFSVSKESLA
                     ETAVYAREKKVYLHTHLCETQDEQDYCLQKLGMRPLDYMESVGWVGPDVWYAHGIWFT
                     PEEIKRLGQMKCGVAHCPVSNLRLGSGICHIPALLEAGVRVGIAVDGSASNDSSNLLK
                     EMQTALLVHRVGTGVTSMPPDKVIRMATRGGAEILGQPEIGQIKPGMAADLALFRLDR
                     IDFAGAMTDPAHALLFCGSAPRAEYTIVAGRVLVEKGRLAGLDEQDVFQRANQLTADL
                     LRKAGH"
     gene            9501..10493
                     /locus_tag="PKI64_05195"
     CDS             9501..10493
                     /locus_tag="PKI64_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00441.1"
                     /translation="MKQLREAVTRERRAPIYWWFALLALTAGGLLFAVWRHTPHGALM
                     LGILVVAAWAAPLVALHSLAAPRRPARPCPAPGTGSFREWQKQARLLGQLLLYYGDVP
                     DLPNALREALYTARADLRDTLRAHPLRDDLERVCARIRAGAVREVKAWFAREYSRDIR
                     ALADEYEQTYTVSMDPDRRLLALQNAVEKAAAMMTRNCMPRMLERERLACATQCAWLA
                     VQGAAQQGRVSPVDLAEMLVIEWSDFTEPWQPAQALARAVARLEPATDTTPAPEPAVV
                     LPPTIKRYRRVRVRSRHRRPRHSHRRNRGPGIGQIMMSFGQWLRYSFRSWFLYR"
     gene            10506..12593
                     /gene="glgX"
                     /locus_tag="PKI64_05200"
     CDS             10506..12593
                     /gene="glgX"
                     /locus_tag="PKI64_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOE00442.1"
                     /translation="MKKRTHVIPHAPVPVPYGAHVLSRGVLFSIVSRHATRVWLMLFN
                     HAEDETPCEEFELLPDNNRLGDLWHLYVPTARAGQYYLYRMDGPRDGRAGHAFDPDQW
                     LLDPYALAVTGQKKWGEHEGITPGQAVKNGRWFPKGIILKDDFDWTGDRTLRVPLAES
                     VIYEANVRGYTAHPSAGVKHPGTYRALIAKIPHLQELGITTLELLPAQEFNEMEFLWE
                     NGARRNLRNFWGYSTMAFFAPNGRYACANQRGEQVREFKEMVLAMHQAGIEVILDVVF
                     NHTAEGGMGGQTFSFRGIDNALYYMLEEDGQRYKNYTGCGNTVNGNHPVVRDFILHCL
                     RYWVLDLRVDGFRFDLATILARGRDGELLANPPVIEQIADDPVLRGVKIIAEAWDAAG
                     AYQVGSFPNKRWSEWNGKYRDDMRDYWRGGASLSTFATRLCGSPDLYDRPRQTPLKSV
                     NYIACHDGFTLADIVAYNEKHNLANCEDNRDGENHNRSDNCGKEGPTDDPAILARRHR
                     RQKNLIATLFLSQGVPMLLGGDEFSRTQQGNNNAYCQDNEISWVDWSLLNTNRALYDF
                     TRRMIAFRQAHPALRRTSFFKGKNIAGDFPDIRWYGPDGAPQPDWEKGVALACRIDGR
                     RQHTGADEEDDHLFLVFNAGPEPQEFELPPNDNGAPWKLALSTQEAEPVVRAKGRPVV
                     TVAGESLAVFTAR"
     gene            12653..13024
                     /locus_tag="PKI64_05205"
     CDS             12653..13024
                     /locus_tag="PKI64_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00443.1"
                     /translation="MNRVGQIGRWLAGVLLAGTVLAGAQVPTDSPQTAAPGPRLTLSS
                     PQLRSTADRINMPWLMPDKLPDFRLKIERPAWDALSVTGLESRVRGIEFQLPIPGFWI
                     GYELPQADEEPRATFSIQRGF"
     gene            complement(13028..13546)
                     /locus_tag="PKI64_05210"
     CDS             complement(13028..13546)
                     /locus_tag="PKI64_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE00444.1"
                     /translation="MNPALAPLFARRSVRKFAAKPVPPELIEDVLRAAMAAPSANGKD
                     PWEIIVVQDPAKLAALATGLPYGKMLAGAPLGFVICADVARANGGLLSYALQDCAALI
                     ENLLLALPMLGLGGVWLGVHPREDRIAHVRELFQLPPDILPISAVAAGYPAVQPPPRT
                     RYSVARVHRETW"
     gene            complement(13552..14130)
                     /gene="def"
                     /locus_tag="PKI64_05215"
     CDS             complement(13552..14130)
                     /gene="def"
                     /locus_tag="PKI64_05215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE00445.1"
                     /translation="MSRILNIHTYGDPVLREPARPVAAITPEIRELVADMYATMRAAQ
                     GVGLAAQQVGRTEAVCIIEIPEEYDREEENGPRLNPDVPLSLVLVNPEIVEKSNETCT
                     MEEGCLSFPDIRGAVERPWRIVVKHLGLDGQPQRTTLQGFMARAAQHEMEHLAGDLFI
                     DHFSHVKRLAIRTKLKRLQTETQDKLLGRETV"
     gene            14214..15545
                     /locus_tag="PKI64_05220"
     CDS             14214..15545
                     /locus_tag="PKI64_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE00446.1"
                     /translation="MKQIGKRRISFAWVAAAWVAVMAIIAATGLFVLQRWHSQAAHQA
                     ATQAVVERGQWLAMSLALRAAGIAGSSDAEAWREFSGLVHSLREVEGELEYVAVRRGD
                     VTLFQEHAAEVSPPAAADDFNFAGPVAIGRQLLAVGEERIPVVTFAVAVTNMAQETVA
                     LELGMRRGAVAREEWPTTRAIESLYRLSLLMIGGCFAVCLALVAWMVRREERREVARR
                     REEHLAFSGVVANGIVHDFRNPMSSLRLDAQMLEKETTRGAEARLPRVAELAGRMRHT
                     LDRMEKVFQEFLILARPGESAPESIELGACAKECVEMLAPRFEAAGITPDVSGIAPDL
                     QIRASAAAVKRALLNILLNATQHAGSGGRVTVTAGRHGAKVRLEVGDTGPGIPKAERQ
                     RVFEMFYTTRPQGTGLGLFLARTAIEKYGGTLAVVDAPEAGARLRVEFPAA"
     gene            15558..16922
                     /locus_tag="PKI64_05225"
     CDS             15558..16922
                     /locus_tag="PKI64_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE00447.1"
                     /translation="MDKARILIVEDDADNGRSLVDAIEDIGYASELTPTAQEGIAAFQ
                     ARGADVVLADLVLPDRDGIAVLQAIRDRDAGRTPVILMTAYASVETSVRAMKEGAYDY
                     LQKPLDIDELQTKLARALEAARLRRQVAELSQEIRGRWSAQSWVAESPAMREVVRQTL
                     ALAQTTATVLIRGDSGTGKELVARALHADGPRAAAPFVAVNCGAFTESLLESELFGHE
                     KGAFTGAVARHAGAFERAHGGTLFLDEVGDAPPPVQVKLLRVLEDKEIVRVGGSQSFK
                     VDVRLVSATNRDLAARVATGEFRQDLLYRLQVVGIALPPLRDRPEDIRPLAERFIAQA
                     QADHGKRIREVEPAWYGRLLAYSWPGNIRELRNAVEAAVVTAPGDVLEAARLRLAGEL
                     AAPPPAAGDWRVPPGLTLEEIEKRVLEESLRQNNGNRSLTAEQLGLSRRTIQRKIQDY
                     NLPF"
     gene            complement(16963..17841)
                     /locus_tag="PKI64_05230"
     CDS             complement(16963..17841)
                     /locus_tag="PKI64_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOE00448.1"
                     /translation="MGLGAVGILFAAPLAARLPGALRVVADAARIARYTADPPSLNGQ
                     RLAFTYVTPDNPGPPADLVLVGVKATNLTDALPAIQAVTGPRTQILPLLNGISAPDDL
                     AAVFGWARVLHGFVYCDSSMRTGHAVRQNGKAKIVFGEVTNAPPSPRVMAVAELLDRA
                     GIRYDIPADMRAAQWKKFILNIGINQAQAFYREPSARLQQDPAAMALARQLMDEAAAV
                     GHALGIAGVQEIPAWAEDVIRAAAPDSKTSMLQDVEAGRPTEVDLFAGTVCRLAAPLG
                     IPVPANEQILRALTAA"
BASE COUNT         3189 a         5193 c         6432 g         3240 t
ORIGIN      
        1 caaaatcggg gctcaaaaaa atggcggaga gagagggatt cgaaccctcg gtaccggttg
       61 ttaccagtac gacggtttag caaaccgttg ctttcggcca ctcagccatc tctccgggca
      121 gggtgggagg taccgtaggg gatgagagcg taatccgcaa gcgtcaaatt tggggcggcg
      181 aaggggtgtg cgcttgtcaa catcggtcgg gtggtcaacg gtcaggagcg ggggtggcgg
      241 taatcttttg acggggaggg gtgtattttg cgataacgct taactcaatg tggcgcaagt
      301 ggtcagtttt gggaatgggc gtttggctgg ggatggccag cgccgggggg ccggcagcgc
      361 gtggagaaat gccgcaaagc gcagagttgc ggcctatcct gggcaatgag ccgacggcgt
      421 tcttgcgcta tgaggtgccg gcggcagtga cgaatgtcca gatttccggc tcgtgggata
      481 tgtggaagct gccgcagccc atgacccggg atgcggcggg gatgtgggtg tttgatgtgc
      541 gcgatttgca ggcgcggctg gggcgctacg agttcaagtt catcgtggat ggggcgtggg
      601 agagtggcgc caaccgcgtg ctgccgatca atctggaggg gattattgaa cgtccgccgg
      661 cgatgatcca gcgcgcggcg attgatgact tccagttgat ccgcgtgttt ttcaaacaaa
      721 gcctgccggc ggggattcag ccgcaagcga cactggaccc gccggtgccg gtggcatgga
      781 cggaagtggt gacggcggag gaggacgcgc ggcgcagcgg gtatttgctg tcgcgcggga
      841 tggtgacgtt tatttttgac caggcggcgt acgggctgga actggcgcgg acgacgcagg
      901 tggtggtggc agggaacttc acggggtggg atggcggcgg gggcgggggc aagtgggtgc
      961 tgaagcgcgg gcggctggcg ggaacgtggg agctggcggc acagttgggc gggatgcggt
     1021 tgccgccggg cgagaaggag ctggtgttca agtttgtgct gaacggcacg gaatggctca
     1081 cgccgccgga agatgcaccg aacgcgctgg cggacggcaa gggaagcatc aatttgaagc
     1141 tggatatttt gcgcagcggc ggcgcggaaa tccgggtgca cacggcggag ccgctggatt
     1201 tgcggcagag ctacatcctg aagctggacg gagtgacgga gcggacgctg ggagtgatga
     1261 cgacgccgga cggggtgttg gaccggttgt atagcgacaa gacgatggga gtgacgctgg
     1321 acaaagtgcg gggcacgacg acgtaccggc tgtttgcgcc gcgggccaag aatgcgtggc
     1381 tgtgtttctt cgatcgcccg ctggcggtgt cgtggacgcc gacattccat cgcttcccgc
     1441 cggcggagga gttccggatg tggcaggacc cggcggatgg cgtgtgggaa atcaccacgc
     1501 gcgggctgga tgtggggcgg ttttacggct tccgcgtgga tgggccgacg ggtgatgggg
     1561 agtcgtttga tccggcggca gtggtggggg atccgtacgg ccgggcggcg gcgaatgccg
     1621 acgggttgac gctggtgatg gatccggacg caaccaacca gtggtttcgc gggtggacgg
     1681 atcaggcgtg ggaaacgccg gacccgcagg acgtggtgat ttatgagtgc cacgtgcgcg
     1741 ggatgacggt gcatccgtcg gcggggattc cggcggcgaa gcgcggaaaa ttcgcgggat
     1801 tggcggcatc gttgggcacg ggcacggggc tggaccacct gaagacgctg ggggtgaacg
     1861 cagtcgagct gctgccggtg agcgagtttt cggagggggc ggacctgtac aactggggct
     1921 atgccacggt gtattttttt gcgccggaat cctcctatgc gcaggagccg gaaaaaggct
     1981 cgggctatta cgagctgaag caactggtaa acgatctgca caaccagggc ttcgcggtga
     2041 ttctcgacat ggtttacaac cacgtgggcg gcccgaatat tttcgagacg ctggataaga
     2101 aatattattt tcgcctgaac acggatttgg gtcactccaa caacagcgcg tgcggcaacg
     2161 acgtgaagac cgaggcgccg atgatgcgca agctgatttt ggacaacatc gaatacttca
     2221 tgcgcgagtt ccatgtggac ggcttccggc tggatttggc ggagctggtt gacatggaaa
     2281 ccatgctcgc catccgcgac cgggcgcggg ccatcaatcc caaggccatc ttgatttcgg
     2341 aaccgtggag cccggggcgc ggcgagaaca aatatcagtt gcgcggtacg gggtggtcgg
     2401 cgtggaacaa cgatttccgc tatgccgcca aggatttcgt gcgcgggcag ggcaaccgcg
     2461 agtggctcaa gggcagcgtg ttcggctcgg tggatctgtg ggcgctgaat ccgctgcaac
     2521 cggtgaacta tttggagagc cacgacgaca tcgcctacat tgacgagctg acggcgaacc
     2581 cggggcgcga cgggcggcaa ttgaccgacc gcgatgcggc gctgaaccgg ctggcggcga
     2641 cgattttgtt cacgtcgctg ggtaaggcga tgctctacga ggggcaggag tttttgcgga
     2701 gcaagtgggg catcaacaac acctacaacc ggggcgatgc cgtcaatgcc gtgcgctgga
     2761 ccgaccgcga ccgcccgctg gcccggcagg cgctggatta ttacgcgggc ttgatccaac
     2821 tgcggatgag cccggaaggc gcggccttcc gcgtggcaca gcgccccccg cgcacgtatt
     2881 accgctggct gttgccggac aatcccaaac tgatgggcta cgtggtcaat gtgcccgagc
     2941 tgcacgcggg gcggggcttt gccgtgctgc tcaatgccga cacccgcgag cgcaccatgg
     3001 atgtggagct gggcggttcc acggcgtgga aaatgatcgg caatggacga gagatcaatc
     3061 cacgcggact gaccccggcc ggctggccgg cgggcaaatc gccgccgagt tggccggcgg
     3121 gctggcgcgg cctgatcgaa gtgcccaagc aagaggccat catcctgatg aacggatttt
     3181 aggcgaggcg tggatggatt tcttgcaaca gttggtggcg cggttgccgg cggagccggt
     3241 gcccgtgcgc ggggtctgga tcgggccgta ctggacagcg gtgcaaaccg cgcgcggcac
     3301 agggttggcc accacgacgc tggctgacgg tcccacgcac ggcccgcacg cgcagcacat
     3361 ggcggcggcg gggaccttgg tggggcgtga cgcgcgcgag ctggcggcgg gggtggcatc
     3421 gtcttttgcg ctggcgcgca gtttggggct ggcggcattg aacgcgctgc tgcccgaacc
     3481 aataggcaag atatccgagc gtaacgcggg cgaattggcg tgcgcgatgg gcgcggggca
     3541 agtggtgggc gtggtggggt ggttcccgtt tatcccacag ttgaaaaagc acgcggcggc
     3601 agtggagatt tttgagaagg acccgcaaac cggctacaca ttgaccaccg agcgcgcgga
     3661 acggctggcc cgctgcgacg gactgtgcgt gacagcctcg gcgctcgtga accaaacgct
     3721 gggcggcatt ttggccgccg tgcagccggg cgcgtggaaa attttggtgg ggccatccgc
     3781 gccgctgtgc gcggaatcgc ttgagcttgg ttttacggcc atttgcggcg cacgggtgac
     3841 cgacaccgac gccgtcgtgc gggtgattca ggaaggcggc tgctttcagc aaatccgcca
     3901 gaccggcggc gtgcgcctgc tgactctgga gacgtgatcc ggcgcgtgcg ggcggtgggg
     3961 gggggctact tgcgcatccg ggcgtacaac gcgcccagca ccagcagcac aatgcctgcc
     4021 aggacgatga atgtgctggg ccgctgcagc agcggcagaa tcggccgcgg cgctttgtat
     4081 tccggcagcg cagcaggatg cagatgccag aggtccgccg gctcgccggc cgccagcgcg
     4141 gcgcggatcg cggagtcgtc aaatgcgggc gcggccactt ggtcgttgtg gtaggcgagg
     4201 gtgtagttac tgcgcgcggt ggaaatccag aacaggcgat aggtggggcc ataggcgcga
     4261 acaccggcaa tgttcaacgg cgcgctgttg gcgttttgga ccaccagccg catctggggt
     4321 gagcgctgct cagggaacgt gacggtgagc tgattggtcg cgtagtcggg cagcagggtg
     4381 gaggagagga tgccatcgcc cacggtgcgc catgtgggtt tgccgtcgcg cacggcggaa
     4441 acctgcacgg tgacaatgcg gctgaagttg ccgggcggaa tatcaaactc gaaccgggtg
     4501 atcggcgcgc caagcgtggt ggcaatgatt tcagtgatct tttgccgctt gtcttgcgcg
     4561 atggttaccg aaaccggcgg ccattcctga agttccagcg cgtcgggcac cagcgtcgtg
     4621 ctgtcgttcc agaggttgat gccggtggtg tggaagggca ttttgagcag ttcgttggcc
     4681 ggcgcggggt cgcgcttgtt gtcgcgcagc agcgcggcca gttgtgcgac ggcatcgggg
     4741 ccggcttcaa caatctcgat ggaaaagaaa cgacaggaat tggcgggcag cgggatttcc
     4801 gtacgccgca tgttgatgaa ctgggaaaag tcgaagatgg ccgcctcggc caagggccgc
     4861 cagaattggt tgtcctcgct gccggagatg cgcacattgc ggacgaagtt ttgctgcgga
     4921 gtgcgcacct ccaggccttg ggcggggggg gcatcgtggg gcagctcaat gaatgcggtg
     4981 gtgatctggt cggagacgac gcgcaagtcg gccttggcgg gggagaggtt gatgcgccgg
     5041 gtttgctcac gggtggagcg gatggcttcc agcacatagg gagtgtcctg cccgccgggc
     5101 tgggtgaaca ggcgcacatc ggtgaagccc ggttgggtca atgcgcacac ggcggcatcc
     5161 aggcggagtt ccgcgaggtg gtcggtttcg ttatacaccg ggaacaggtt gcgcgtgatt
     5221 ccgaaatcgg ccggggcaac cgcgttggcc atcgtggcgg cgatcagcag gctggcaaac
     5281 acaaggcgtt tcatgggcaa gctcctcatg cggcaactat ccactatccg gggcgggttg
     5341 tccatgtttc cggcgcagga aaatgccttg ctggacaggg gggatcgtga gagtttttca
     5401 cggaagaacc gcaagatgaa accggagcgc atgattccaa cgtgggccgt ggtgggtggc
     5461 gggtggctgg tggtgtggct ggcgttggcg gtgggggggc gcgggccgct ttggccgccg
     5521 gaacttcccg cgcagccggt gggcggggac ttggccatga tgctgggcta cgcgcagtca
     5581 tggttggcca cgggggatcc ctacgctgcg ctgaatccat atccgccgct ggccgcagtg
     5641 ctgtttgcgc cgctgacgaa actgccgttt gcggcggcgc tggcggtcat gactgcgttg
     5701 acgctttttg tgtttgtggt caccaccgcc tggctgccgc ggctggtgtg gccgcgcgcc
     5761 gagcgcaagg cggtgtggct ggtggcgctg gcggacttgt tttcctatgg actatttttt
     5821 gagctgcgct gggggcagtt caatgtggtg gcgctggggt gtgcggcatg gggcattttt
     5881 tgcttccacc gggcgtgtgg cccggcgatg cgcttggtgg gctacgcgct cttttgcgtg
     5941 ggtgtgcagt tgaagttgta tccggcgctg ctggtttttc tgttcaccaa ggatgcgcgc
     6001 gcgtggggcg tgaatttatg gcgatgggca gcgctgggag cggtcaatgc ggcgctgttg
     6061 tttgcgctgg gtggggcggt ggcgcaggcg tttggtcatt cggtgcaggc acaggccgcg
     6121 gcgccgtatg tgtgggtggg gaaccactcg ctgcaaagtt gggcggcggg ggtaggccag
     6181 ccggcggtcg tgccgtggtt gttcgcgggg tatgcgctgt gcctcgggtg gggtttggcg
     6241 cgggcgtggt ggcaaccgac ggcgatccca gttgccaatg cggccttgct gggcgtactg
     6301 gggatgctgc tgctgccggg cgtgagtcac gattacaaat tgtccctgtt gccgatgggg
     6361 tttgcgggtg tcgtggcggc gcatggtgcg ggcttggtgt ggcggcggga aaatctcggc
     6421 cggttgctga cgctggcggg agtgagcgtg atggtggctt ggacgttgtt cccaccggcc
     6481 gccaaaccgg ccgccttgca gaataatgcg ccggctttgt tggcgggttg tattttgttg
     6541 gcgtggggcg ggggtggcgt aagccggaag gacggtggaa aatgaaattg gaacgggtga
     6601 cggggccttc acaaatcgca gcggtggcgg aactggcccg tgagatttgg accgcgtatt
     6661 acacgccgct gattggcgcc gcacaagtgg gctacatgtt ggcggcattc caaagcgcgg
     6721 aggccattac gcggcaaatc ggcgcggaag gctacgagta ttaccttgcg ccgggcgagg
     6781 gctatctggc gctggtgccg gaccccgcca aacaaagtat gtttttgagc aagatttacg
     6841 tgaaggaaca ccttcggcgg acaggcctcg ggcgggccat gatggagttg gccgaggcgc
     6901 gttgccgcga aactggctgc cgtgagctgt ggctgacggt gaatcgccac aatgcgggga
     6961 gcatcgcgtt ttacgagaaa atgggttttc ggaaaaccaa cccgctggtg acggatatcg
     7021 gcggcggctt cgtaatggac gattggcgca tggccaaaac catccccttg gcggcagcaa
     7081 atccaacgga gtgaagaaaa gaaagacact cgccaagacg caaaggacgc ctagatggat
     7141 gagagtgagt ggcttcccga tggaatgaca tccttggcgg tcttggcgtc ttggcgagag
     7201 aaaaaccacg tgatttcccc tttgcgcggc ggggccagca tccgccgcca aaaagacgaa
     7261 gcgcttgacc cggcgggggg cacgggatag gctgactcat catgggcgcg atgcaggagt
     7321 tggatcggga agccgcattg gcggtggcgg tggcggcggt caaagaggcc ggccgtctgc
     7381 tgctggagtt gcgccaacaa cccgtggaag tgctgagcga gccgggtcac gacatcaagc
     7441 tcaaggccga tcaactggcc gaagaacgca ttttggccat tttgcacgaa cgttttcctt
     7501 tgccggtgtt gacggaggaa agcggcgaac atggcgcggt gaacgagtcc ggccggatgt
     7561 gggtggtgga cccgctggac ggtaccttca actactcgcg cgggatgccg ctgtgctgct
     7621 cagccatcgg cctctgggaa aatggccaac cggtgctggg cgcggtgtat aacttttttc
     7681 gcgatgagct gttcacgggg atcgtggggc agggggcgtg gcgcaatggc gagcgggtgc
     7741 gcgcgtcggg cgaaacgcgc gtggccaagg cctcgctggc gacgggtttc ccgcatcagc
     7801 agagctatga agaagcgccg ttgctggcgt ttgtgcgcaa tgtccagcgg ttcaaaaaaa
     7861 tccgaatgct gggctccgca gcgctgatgg gcgcgtatgt ctcgtgcggg tggttggatg
     7921 gctatgtgga agaagatgtg tggttgtggg atattgccgg cgcggtggct gtggcgaagg
     7981 cggccggcgc ggtggtgacc gtgcggccgg gcaaggcagg gcgctgggcg cgcgaagtcg
     8041 tgtgcgcggc ctcgccggaa ctgcacgcgg ccttggccgc cccggcgcca tgatgcggcg
     8101 atgatgttga ggagaaggcc atgagcaaac tgaaaatcga aaaaatccac acgctggtga
     8161 cggtggatga cgactaccgc gtgctgcatg atgtggatgt ggtgctggcg ggcgacaaga
     8221 tcgaagccgt ggggccgaac ctgcccacgc cggaaggcgc gcgcgtgctg gatggccgct
     8281 ggtgcgtggc ctatccgggc tttgtcaaca cccaccacca tttctaccag acgctcaccc
     8341 gcaacattcc ggcggtgcaa aactccaagc tctttgattg gctgctgtgg ctctacgaag
     8401 tgtggcgtgg gctgcacccg gaagcggtac gcgtcagcac gcaggtggcg ctgggcgagt
     8461 tgctgctgag cggctgcacc accagcaccg accattttta tgtgttcccg cagtccgcgc
     8521 cgcaggagtt tttggacatc gagattgata ccgcgcgcga gattggtgtg cgcttccatc
     8581 ccacgcgcgg cagcatgagt ctcgggcggt cgcagggcgg cctgccgccg gacgatgtca
     8641 cccaaagcgg cgaggccatc atggccgact gcgagcggct gatccgcaaa tatcatgatc
     8701 ccaagccgtt cgccatgacg cgcattgtgt tggcgccgtg ctcgccgttt tcggtcagca
     8761 aggaatcgct ggcggaaacc gcggtctatg cgcgcgagaa aaaggtgtac ctgcacaccc
     8821 acctgtgcga gacgcaggat gagcaggatt actgcctgca aaaactggga atgcgcccgc
     8881 tggattacat ggagagcgtg gggtgggtgg ggccggatgt atggtacgcc cacggcatct
     8941 ggttcacgcc ggaagaaatc aagcgccttg gccagatgaa gtgcggcgtg gcgcattgcc
     9001 cggtgagcaa cctgcgcctc ggctcgggca tttgccacat tccggcattg ctggaggccg
     9061 gtgtgcgggt gggcattgcg gtggatggca gcgccagcaa tgattcctcc aacctgctca
     9121 aggaaatgca gacggctttg ctggtgcacc gcgtggggac gggggtgacc tccatgccgc
     9181 cggataaagt gatccgcatg gccacgcgcg gcggcgcgga gatactgggc cagccggaaa
     9241 tcggccagat caagccgggg atggcggcag acctcgcgct gttccggttg gaccgcattg
     9301 attttgccgg cgccatgacg gatcccgccc acgcgctgct gttctgcggc agcgcgccgc
     9361 gtgcggaata caccattgtg gccggccgcg tgctggtgga aaaaggccgc ctggcggggc
     9421 tcgacgagca ggacgttttc cagcgcgcca accaactgac cgccgatttg ctgcgcaaag
     9481 ccggacacta accgcaatcc atgaagcagc tacgggaggc cgtgacgcgc gaacgccgcg
     9541 cgcccatcta ttggtggttt gcgctgctgg cgctgacggc cggcggcctg ttgtttgcgg
     9601 tttggcggca cacgccgcat ggcgcgctga tgttgggcat tctggtggtg gcggcgtggg
     9661 ctgcaccact ggtggcgctg cattcgctgg ccgcgccgcg ccgcccggcg cgcccgtgcc
     9721 ccgcacccgg cacgggttcc ttccgcgagt ggcaaaagca ggcgcggcta ctgggccaac
     9781 tgctgctcta ttacggcgat gtaccggatc tgcccaacgc cctccgcgaa gccctctaca
     9841 ctgcgcgcgc cgacttgcgc gacactctgc gggcgcaccc gctgcgtgac gatctggaac
     9901 gggtttgcgc gcgcatccgg gccggcgcgg tgcgggaggt gaaggcgtgg ttcgcgcgcg
     9961 aatacagccg cgatatccgc gccttggccg atgaatatga acagacctac accgtcagca
    10021 tggaccccga ccggcggctg ctggcgctgc aaaacgccgt ggaaaaagcg gcggccatga
    10081 tgacccgcaa ctgcatgccg cgaatgttgg agcgcgagcg cctcgcctgc gccacgcaat
    10141 gcgcgtggct ggccgtgcag ggagcggcgc aacaagggcg ggtctcgccg gtggatctgg
    10201 ccgaaatgct ggtgattgag tggagcgact tcaccgagcc gtggcaaccc gcgcaggcgc
    10261 tcgcccgcgc cgtggcccgc ctcgagccgg ccaccgatac aacacccgcg ccggaaccgg
    10321 ctgtggtcct gccgccgacc atcaagcgtt accgtcgtgt gcgcgtgcgc tcccggcacc
    10381 gccggccgcg ccactcgcac cggcgcaacc gcgggcccgg catcggccaa attatgatgt
    10441 catttgggca gtggttgcgc tattccttcc gctcctggtt cctctaccgt tgagaaagaa
    10501 caccgatgaa aaaacgaacc catgtgattc cccatgcccc ggttcccgtg ccctacggcg
    10561 cgcatgtgct ttcgcgcggc gtgttgttca gcattgtcag ccggcatgcc acccgcgtgt
    10621 ggctgatgct cttcaaccac gccgaggacg agaccccgtg cgaagagttc gagctactgc
    10681 ccgacaacaa ccggctgggt gacttgtggc acctgtatgt gcccaccgcg cgcgccgggc
    10741 aatattatct gtaccgcatg gatggtccgc gcgatggccg cgccggccat gccttcgatc
    10801 ccgaccagtg gttgctggac ccctacgcgc tggccgtcac cggccagaaa aaatggggcg
    10861 aacatgaggg catcacgccc ggccaagccg tcaagaatgg ccgctggttc cccaagggta
    10921 tcatcctgaa ggatgatttt gattggacgg gcgaccgtac gctgcgcgtg ccgctggcgg
    10981 agtcggtgat ttatgaggcc aacgtgcgcg gctacacggc gcaccccagc gccggggtga
    11041 agcatccggg cacctaccgc gctctgattg ccaaaatccc gcatttacag gaactgggca
    11101 tcaccacgtt ggagttgctg cccgcccagg aattcaacga gatggaattc ctctgggaaa
    11161 acggcgcgcg gcgcaacctg cgcaacttct ggggctatag caccatggcg ttttttgcgc
    11221 ccaacggccg ctacgcctgc gccaaccagc gcggcgaaca ggtgcgcgag ttcaaggaaa
    11281 tggttttggc gatgcaccaa gccggcatcg aagtcatttt ggatgtcgtt ttcaaccaca
    11341 ctgccgaagg cggcatgggc gggcagacgt tctccttccg cggcattgac aacgcgctct
    11401 actacatgct ggaagaagat ggccagcgct acaaaaacta caccggctgc ggcaacacgg
    11461 tgaatggcaa ccatcccgtg gtgcgcgatt tcatcctgca ttgcctgcgc tactgggtgc
    11521 tggatttgcg cgtggacggc ttccgcttcg accttgccac catcctcgcc cgcgggcgtg
    11581 acggcgagct gcttgccaat ccgcccgtca ttgagcaaat cgccgatgat cccgtgctgc
    11641 gcggggtaaa gatcatcgcg gaagcgtggg atgccgccgg ggcgtatcag gttggctcgt
    11701 tccccaacaa gcgctggagc gaatggaacg gcaagtaccg cgacgacatg cgcgactact
    11761 ggcgcggtgg cgcttcgctg agtacgtttg ccacccggct ttgcggcagc cccgacctct
    11821 atgaccgccc ccgccaaacc ccgctcaaga gcgtcaacta catcgcctgc catgatggtt
    11881 tcacgctggc cgacattgtc gcctacaacg aaaaacacaa cctggccaac tgtgaggaca
    11941 atcgcgacgg cgaaaaccac aaccgcagcg acaactgcgg caaggaaggc cccacggatg
    12001 atcccgccat tctggcgcgg cgccaccgtc ggcagaaaaa cctgatcgcc acgctgttcc
    12061 tctcgcaggg ggtgcccatg ctgctcggcg gcgacgagtt cagccgcacg caacaaggca
    12121 acaacaacgc ctactgtcag gacaacgaaa tttcttgggt ggattggagc ctgctcaaca
    12181 cgaaccgcgc actctacgat ttcacgcggc gcatgattgc gttccggcag gcgcatcccg
    12241 cactgcggcg cacgtccttc ttcaagggca agaatatcgc tggcgacttc cccgacatcc
    12301 gctggtacgg ccccgatggt gcaccgcagc ccgattggga aaaaggcgtg gcgctggcct
    12361 gccggattga cggtcgccgt caacacaccg gcgcggacga ggaagacgat cacctgtttc
    12421 tggtgttcaa cgccgggccg gagccgcagg agtttgagtt gccgcccaac gataacggtg
    12481 ccccgtggaa actggcgctg agcacgcagg aagccgagcc ggttgtccgc gccaagggcc
    12541 ggccagtggt gaccgtggcc ggcgaaagcc ttgcggtgtt caccgcgcgc tgacgaaccg
    12601 cacgcgggct ttggggtggc ggggtgcggg cggaagggat acactgccgc gcatgaatcg
    12661 ggttggacag attggtcggt ggctggccgg cgtgttgctg gccggtaccg ttctcgctgg
    12721 cgcacaagtg ccgacggact ctccccaaac cgctgcgccg gggccacgac tcaccttgag
    12781 ttcgccacaa ctgcgctcca cggcggatcg aatcaacatg ccgtggctga tgcccgacaa
    12841 actgccggac ttccgcttga aaatcgaacg gcccgcgtgg gatgcgttat cggtcaccgg
    12901 gctggaaagt cgggtccgtg gaattgaatt tcaactgccg attccggggt tttggatcgg
    12961 ctatgaactg ccgcaggccg atgaggaacc ccgcgccacg ttttcgattc agcgcgggtt
    13021 ttgacgttca ccacgtctcg cggtgcacgc gcgccacgct gtagcgcgta cgcggcgggg
    13081 gttgcacggc gggataaccc gccgccaccg cgctgatggg gaggatatcg ggcggcaatt
    13141 ggaacagctc gcgcacatgg gcaatgcggt cttcgcgcgg gtgcacgcca agccacacgc
    13201 cgcccagccc cagcatggga agcgccagca acaggttttc gataagggcc gcgcaatctt
    13261 gtagcgcgta ggagagcagg ccgccgttgg cccgcgccac atcggcgcaa atcacaaaac
    13321 cgagcggcgc acccgcgagc attttgccgt agggcaggcc agtggccagt gcggcgagct
    13381 tggcggggtc ttgcaccacg atgatttccc acgggtcctt gccgttggcc gatggcgcgg
    13441 ccatcgccgc ccgcagcaca tcttcgatca actccggcgg gacgggtttg gccgcaaact
    13501 tacgcacgct gcggcgggcg aacaatgggg caagggcggg gttcattcgc gtcagacggt
    13561 ttcgcggccg agcagtttgt cctgggtttc cgtttgcagg cgcttgagct tggtgcggat
    13621 ggcgagtcgc ttgacgtggc tgaagtggtc aatgaacaaa tcgccggcca gatgctccat
    13681 ctcgtgctgc gcggcgcggg ccatgaagcc ctgcagggtg gtccgttgcg gctggccgtc
    13741 gaggccaagg tgcttcacca cgatgcgcca cggacgctcc actgcgccgc ggatatccgg
    13801 aaagctcagg cagccttcct ccatggtgca ggtttcgttg gacttctcga caatctccgg
    13861 attcaccaga accagcgaga gcggcacatc gggattcagc cgcgggccat tttcttcttc
    13921 gcggtcgtac tcctcgggaa tctcgatgat gcacaccgct tccgtgcgcc caacctgttg
    13981 tgcggccaaa ccaacgccct gcgccgcgcg catggtggca tacatatccg caaccagctc
    14041 ccggatttcc ggggtgatgg cagccacggg gcgggccggt tcgcggagaa ccggatcgcc
    14101 ataggtatga atgttcaaga tgcgactcat gccgtgggca agatactgca caacagggcg
    14161 aaaacaaacg gaaaaacttg ctcgaagacg gggcggggct agaatgcttc cgcatgaaac
    14221 aaatcggcaa acggcggatt tccttcgcgt gggtggcggc ggcctgggtg gccgtcatgg
    14281 ccatcattgc ggcgacgggg ctgtttgtgc tgcagcgctg gcatagccag gcggcgcatc
    14341 aggccgcgac gcaggcggtg gtggagcgcg gccaatggct ggccatgagc ctcgccttgc
    14401 gcgcggccgg cattgccggc agcagcgatg ccgaagcctg gcgcgagttt tccggtttgg
    14461 tgcattcctt gcgcgaggtc gaaggcgaac ttgaatatgt cgccgtccgc cgcggcgatg
    14521 tgacgctctt ccaggagcac gccgcggaag tcagcccgcc cgcggccgcg gacgacttca
    14581 attttgccgg cccggtcgcc attggccggc agctattggc cgtgggcgag gaacgcatac
    14641 ccgtggtgac gtttgccgtg gccgtgacga acatggcgca ggagaccgtg gcgctggaac
    14701 tcggcatgcg gcgtggtgcg gtcgcccgcg aggagtggcc caccacgcgc gccattgagt
    14761 cgctctaccg gctaagcctg ctgatgatcg gcggctgttt cgcggtttgt ctggcgctgg
    14821 tggcgtggat ggtgcgccgc gaagagcgcc gcgaagtagc ccgccgccgt gaagagcatc
    14881 tggccttttc cggcgtggtg gccaacggca tcgtgcacga tttccgcaat ccgatgtcca
    14941 gcctccggct ggatgcccaa atgctggaaa aagaaaccac ccgcggcgcg gaagcgcgcc
    15001 tgccgcgcgt ggccgaactg gccgggcgga tgcggcacac gctcgaccgc atggaaaaag
    15061 tattccaaga atttctgata ttggcgcgac cgggcgaatc cgcgccggag agcatcgagt
    15121 tgggcgcatg cgccaaagag tgcgtggaga tgctcgcgcc gcgctttgaa gccgccggca
    15181 tcacgccgga tgtgtccgga atcgcgccgg acttgcagat tcgcgcgtcc gccgccgcgg
    15241 tcaaacgcgc cctgctcaat attttgctca acgccacgca gcatgctggc agcggcgggc
    15301 gcgtgacggt gaccgccggc cgtcacggcg cgaaagtacg cttggaagtc ggcgataccg
    15361 gccccggcat tcccaaggcg gagcgccagc gcgtgttcga gatgttttat accacgcgcc
    15421 cgcaaggcac gggcctcggc ctgtttctgg cccgcaccgc catcgaaaaa tatggcggca
    15481 cactggccgt ggtggatgcg cccgaagccg gcgcgcggct gcgcgtggag tttcccgcgg
    15541 cataacgagg agatagcatg gacaaggcgc ggattttgat tgtggaagac gatgccgaca
    15601 acgggcgctc gctggtggat gccatcgagg acatcggcta tgcctccgag ctgaccccga
    15661 ccgcgcagga aggcattgcc gcgtttcagg cgcgcggcgc ggatgtggtg ctggccgatc
    15721 tggtcctgcc cgaccgcgat ggaatcgccg tgctgcaagc catccgcgac cgcgatgccg
    15781 gacgcacgcc ggtgattctc atgaccgcct acgcctcggt ggagaccagc gtgcgtgcca
    15841 tgaaggaagg cgcctacgat tacctgcaaa agccactgga tattgacgag ctgcaaacca
    15901 agttggcccg cgcgctggaa gccgcacgct tgcgccggca ggtggccgaa ctgagccagg
    15961 aaatccgcgg ccgctggtcg gcgcagtcgt gggtggcgga atcgcccgcc atgcgtgagg
    16021 tggtgcgcca aacactggcg ttggcgcaga ccaccgccac ggtgctgatc cgcggcgaca
    16081 gcggcaccgg caaggagctg gtggcccgcg cgctgcacgc cgatggcccc cgcgccgccg
    16141 cgccatttgt ggcggtgaac tgcggcgcgt tcacggaaag cctgttggaa agtgagctgt
    16201 tcgggcatga aaaaggcgcg ttcaccggcg cggtggcccg ccatgccggc gcgttcgagc
    16261 gcgcgcacgg tggcaccttg tttttggatg aagtgggcga tgcgcccccg ccggtgcagg
    16321 tgaaactcct gcgcgtactg gaggacaagg aaatcgtacg ggtggggggc agccaaagct
    16381 tcaaggtgga tgtacggctg gtgtcggcca ccaaccgcga ccttgccgca cgcgtggcca
    16441 caggcgagtt ccgtcaggat ttgctctacc gcctgcaagt ggtgggcatt gcgctgccgc
    16501 cgctgcgcga tcgtccggag gacattcgtc cgctagccga acgcttcatc gcgcaggcgc
    16561 aggccgatca cggcaagcgc atccgcgagg tcgagcccgc ttggtacggc cggttgctgg
    16621 cctattcctg gccgggcaat attcgcgagc tgcgcaacgc cgttgaagca gcggtggtca
    16681 ccgcccccgg cgatgtgctg gaagccgcgc gcctgcggct ggcgggcgag ctcgctgcgc
    16741 cgccgccggc agccggcgac tggcgcgtac cgcccggcct cacgctggaa gaaatcgaaa
    16801 aacgcgtgct ggaagaatcg ctccggcaaa acaatggcaa ccgcagtttg acggcggagc
    16861 aactcggcct gtcgcggcgc accatccagc gcaagattca ggattacaac ctgccgtttt
    16921 gagccgtcgc gcgcccgcgg gatggccgct ggatattcgc gttcaggcgg cggtcagcgc
    16981 gcggagaatc tgctcgttgg cgggcacggg gatgcccagc ggcgccgcca gccggcacac
    17041 ggtgccggcg aacaagtcca cttccgtcgg gcggccggct tccacatctt gcagcatgga
    17101 tgttttgcta tccggcgcag ccgcacggat gacatcctcg gcccacgcgg ggatttcctg
    17161 cacgccggca atgcccagcg cgtggcccac ggcggcggct tcatccatca gttgccgggc
    17221 cagcgccatg gcggcggggt cctgttgcag acgcgcggat ggctcgcgat aaaatgcctg
    17281 cgcctgattg atgccgatgt tcaggataaa ctttttccat tgggcggcgc gcatgtcggc
    17341 ggggatgtcg tagcggatgc cggcgcggtc cagcagttcg gccacggcca tgacgcgcgg
    17401 ggaggggggg gcgttggtga cctcgccgaa aacgattttg gccttgccgt tttgccgcac
    17461 ggcgtgaccg gtgcgcatgg agctgtcgca atagacaaag ccatgcagta cacgcgccca
    17521 gccaaagacc gcggccagat catccggcgc ggaaattccg ttcagcagcg gcagaatttg
    17581 ggtgcgtggt cccgtcaccg cttggatggc cggcagcgcg tcggtcaagt tggtggcctt
    17641 cactcccaca agcaccaggt ccgccggcgg gccgggattg tcgggtgtga cgtaggtaaa
    17701 ggccagccgt tgaccgttga ggctgggcgg atcggcggta tagcgcgcga tgcgggcggc
    17761 atcggccacc acgcgcaacg cgccgggtag ccgggcggcc agcggcgcgg caaacagaat
    17821 gccaaccgca ccaagaccca cgagggctac ggagttccgg ggagcagcag ttggcggttg
    17881 ttggaccatc actggaaact aaccgagaac cgcgtcgcac gcaacccgcc gggcacgcga
    17941 aaaaacttca aaaaagtggc ttccccggat cttttgtagg gcgggaatgg ttgaagaatg
    18001 aaaccttccc gccgggaacg gctggaaggt ctgcgaccgt tccagccctt ctat
//
LOCUS       DAOPNP010000040        17963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3750_length_17963_cov_9.271220, whole genome shotgun sequence.
ACCESSION   DAOPNP010000040 DAOPNP010000000
VERSION     DAOPNP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3750_length_17963_cov_9.271220"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..557)
                     /locus_tag="PKI64_05235"
     CDS             complement(18..557)
                     /locus_tag="PKI64_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00449.1"
                     /translation="MIEERSLPAGKGWKVCNHSSPVIQPEAVFGAGRLKLVEAVNSRR
                     PHRDRLQKQPQNGQKQEKQAVFEGFLRFSSLKLAETQDVLPVAVLGGVDVFCMKKSIP
                     KPTAAVNSRRPQRDRLQEQPRGNRLPDCAHCPPTLEPAQEGVPPKNSQLTTLAVPIAA
                     FLFYYNDAVASQRLDKTAM"
     gene            681..1007
                     /locus_tag="PKI64_05240"
     CDS             681..1007
                     /locus_tag="PKI64_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOE00450.1"
                     /translation="MIEGWITQLRKGVLEYCVLLVLRRGESYGYELVQALQASDDLAV
                     SESTVYPILARLKQEGYLKARDVPSAAGPPRRYFSLTPRGKVRLSEMNAYWPVLMSAL
                     EKLKAK"
     gene            1025..6442
                     /locus_tag="PKI64_05245"
     CDS             1025..6442
                     /locus_tag="PKI64_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOE00451.1"
                     /translation="MKWSMEAKQRVEDYLHEVERHLAHKPESVRKDVVEGLRNQISES
                     VQRLEIIGGVIGPEVIEQILAGMDSPESFAEAALEVVGAAATELAPTAIRSGGGRWFA
                     LALAFLAINAYGVWKWTRTLAPPSPAPTAETAAIPPAPKPIERVLRLRQIGQVDVSAQ
                     REVTLKLAFNAAPDRTQLTRFLRLTAEGQGEVKYWLLGGVNAEEAVIETEPVLAEKLE
                     YRLEPGLPAKSEDIQPEDRLREGSLRMEMNLQLREVEARSPSFEQPYISAEFNAVPNA
                     EKLAEYVSVKPAVDFTVEIMRRWWETGMRIRGDFRPGTIYDVTFKKGLPAANGSSLAQ
                     ALTRSVQIPEPGPAVKLDNNGYYLSPRGTLRVPVAAVNLKEYVARLQPVFINNLVPLT
                     LADREQVYFRNDQDLYGEAQALTNRLPAAAPGEIVRTSLDLRALAGGELRGAYALRVE
                     ADSDRGEDRIIVVTDLGIATKLHDGGALVWVNSLRDAQPVAGATVTVLARNNQPLARG
                     ETDGQGLLRLEWAQTDTARPCIVVAERDGDLSYINLERTRNYPGEGTSGAAYLADGKL
                     EAAVFTERGIYRPGETVFMQALVRDNQLRAPEAIPVLWLVVQPDGRVYREIPATLDAF
                     GSAQATVVLPDYLKTGRYSIQLAMPGTRTVLGSTKVSLEDFVPPQIRVKVTAPEGRHT
                     AGSALGSRVRAEHLFGRPAVGLKAEGWAVFTPVEFAPTNWPGWTFGDPERQFDGEEYE
                     QGVQNLDDDGVAEFEAVSRKAWRPPAALQLVQQVTVTEANGRGVTAYDRTLIDPYPFY
                     LGLQPAWEGGAVRVGQTQRVAVVEVQPDGTGVAKGKPVALALARVSWNSVLRRNDNGR
                     YEWKSEKQTVVIRRDTLAAGGTPQDWTFAVDTAGDYELVATELASGAQTRLSFFAGSP
                     EQEWQAWSREKPGRVELAWDKKTYRPGDTARLQVRAPFAGPALLTVETDRVREARVVM
                     LEKNTAEFEVPVTAAQVPNAWCTMTLIRPAQAEAVWSAHRASGVIALVVDPPEPHLAV
                     TVDAPARVLPQTPLQATITVRDHAGQPAQGAVTVMAVDEAICMLTDFATPDPAAEFLR
                     CRGLGVNDYDLYAELMPVTEDAVETVPRAGGGAGAELRRRLNPIKANRFKPLALWQAD
                     LPLDGDGRATAQFDLPEFNGEVRLMVVAYNAAQTGATSMPVKVKRDLVVQSALPRFMA
                     IGDRSDAGVVLYNESGAPLTATVRALCGGPLRAENAELTVALPTGGSTNLVVPLVAGP
                     GPGVARFSLEVSGGGEEFRDSIELAVRPASGTRSLSVQRVVAPGEEAAFTAPADWLPA
                     SVALTGVLSALPSLEMGRALDYVVNYPYGCLEQTVSGAFPLLYAGEWTQRMLPSATAA
                     GDIPGMVDAAIRRVLNMQQNNGGFAVWPFTSDVEEDVSLYATHFLVEAQAAGFTVPAD
                     RVQAALNRLKNWLDRSIPAKATPEQMADDLQTRAYICHILARAGQPHEGWNARLREQA
                     DQLNFAARAHTASALLLAGEPRQALPLLEQTKLPTSRPHLAGDWRASDIREAALMLSA
                     WLDVDPENEAVARLAQYLRNRQQNGHWGNTQDNAMALLAFGKLARALPVKEQPFAGRF
                     TVADHTQTFGTTNDVQYTSAPGEGGTLTVHNDGPGKMYAWVQFTGVSTEPEPPHTNQL
                     ALSREFLDRQGEPLAVNSLPQGDLFVVKITVDPRGQPLDHVVIEDLLPAGWEIENPNL
                     QTAQNLPWLKDKGDKPRHTEARDDRMLIFSGRMQKPATYYYSVRAVTPGEYTLPALTA
                     VAMYDPEISAATASSKVRVVP"
     gene            6439..8661
                     /gene="pbpC"
                     /locus_tag="PKI64_05250"
     CDS             6439..8661
                     /gene="pbpC"
                     /locus_tag="PKI64_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOE00452.1"
                     /translation="MTSRRVKWLRRSAFAVAALTAGSYAAVCSVPFPEADLARLPAAV
                     VLADRTGEPLRLKLAAGGYDCRPGYAPDPAHWIGKAIVAAEDQRFWRHPGVDVLALAR
                     AVADNVALGRRVSGASTISTQVIRLAQPRQRTLGTKCIEAFRALQLERRYDKQTILGM
                     YLDRAPFGGNLVGIEAAARHYFAKRPADLSLAEAALLAGLPQSPSRLRPDRHLARARQ
                     RQAYVLERMEACGMITADERAAAQAQPLAFQRAPRPFAAPHFCEMVGVPARTADGAPL
                     RTTLDLRQQRLAEDVLARHLQRSPINSGAIVLLEVRTGAVRALVGSPDYFARETAGQV
                     NGATARRAAGSTLKPFAYALALDRGFITPGMMIPDIPTRYRDLDPRNFSPEYRGLVSV
                     RDALMLSLNLPAIEVEKRVGQERFWSTLRALGLGTIDQPAAHYGLGLVLGNTEVRLLD
                     LANAYACLARGGVWFPPRLLENSRPPAGRELFSPEACWLVAEMLSGEERTMDIAGHAA
                     DVRLPPMAWKTGTSAGLRDAWTVAWNPDYVIGVWLGNADGSPADELVGRLSATPIAWE
                     VFRQLYPDNNGPWYARPPGIIERNVCAVSGCVPGANCPHAVQDWAIAKVTRHEPCPVH
                     RQGPAETWPVAVASFLARRTAPAADESAAALRIRSPARGSTYRWLPDLTVDAQRLALD
                     AASDRAGETLHWFVNDQPIGRSRAGEPLFWPLTPGTHTIVCSTARGLSDRIQIAVETQ
                     "
     gene            8739..9527
                     /locus_tag="PKI64_05255"
     CDS             8739..9527
                     /locus_tag="PKI64_05255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00453.1"
                     /translation="MKISIVTANYNGGRWLARCMDSVLNQELAPGDELEYILIDGAST
                     DDSLQIAQARRGRLAALISEKDHGPADALNKGFALATGDLLGWLNADDVYEPGALARA
                     LAAAKARPQAAFFFGKCRIVDEAGRDIRSFPTLVKNACMPFSSRFLIQCINYVSQPAS
                     WFRCAALAAAGGLRLDFKAAWDYDLTLRLWRQGGGVRIPGPPLAAFQWHPGSISGTHY
                     EQQFAEEWRAAVADAGRFSPQALVHWFVRHGIVLCYRLMNRPRA"
     gene            complement(9556..11361)
                     /locus_tag="PKI64_05260"
     CDS             complement(9556..11361)
                     /locus_tag="PKI64_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE00454.1"
                     /translation="MGNELAGDILIERFDVIACSKCNQPIDTSQAAPFSLLNCPVCQA
                     EMRVPARFANFILLEQLGKGGMGAVYKAYDETLGRTVALKVMQQAIGQDPAFVAQLQQ
                     EARALAAINNPHIVQIYSYGEENGQPYIVMELVDGGRLDHMQEKKKQLDETFVLRMAT
                     QIIRGLQAANAAGMTHGDIKPANILFDRAGNAKVADFGLARFKGQKPQPGEVWGTPYY
                     VAPEVVKGQAPNAGSDIYSLGGTLYHVLTGEPPFNCETVTDTVLLRFKEPAPDPRVFK
                     NNISARTAAILLRMLEMDSFARYPTYESLLKDINDALEPLTAAQSGKKPEAARRNLVV
                     PVVLGIVGVALIAGLTVVGKSIYKDKRKQAQEEAQRQADIAAGRLKQVMRGGQLVWVR
                     VDTAAPAAGGAAAPAAPINDWTIPASMDVSISGDNSSGDFKVQNILPLHGGTGDLAEA
                     SKIYLRFPLTGVDKARLDQAVLRLTFSKTTPVKPKTKSYTLQVWGLKTAAVWDESITW
                     QTAPGNDPASTGNLMPTQATLLANYPLPPNPQTGDILTVEDRALTKFLREFPGDEVTL
                     VLTAGSDGDHKKGWWITATEDSQRFRPPTLILKAK"
     gene            complement(11371..12318)
                     /gene="argF"
                     /locus_tag="PKI64_05265"
     CDS             complement(11371..12318)
                     /gene="argF"
                     /locus_tag="PKI64_05265"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOE00455.1"
                     /translation="MRHLLTLADWNQDQIAEVIALARTIKAHPADYADSLKRKTLCMF
                     FEKPSLRTRLSFEAGMNQMGGHAIYYDTSTSPFGSGKESIADMVRTTCRYVDIIMARL
                     FKHEDLLEMARYTTVPIINALTNFSHPGQILADLQTVEEHLGKLAGLKLAYLGDSFNN
                     VTHSLLFAVPKMGMHLAVACPTGADYEPAATARAAAEADAARTGTRFDWGHDAQAAVR
                     DADVVYTDSWMSYHIPKEKAGERMQIFAPYRVTSELMKLAKPSAIFMNCLPAMRGMEQ
                     TAEVIDGPQSVVFDQAENRMHMHKALMLLLLREAGRIGV"
     gene            complement(12440..12832)
                     /locus_tag="PKI64_05270"
     CDS             complement(12440..12832)
                     /locus_tag="PKI64_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00456.1"
                     /translation="MDHGIMERDWKYMCKIQGELLNTLYEQINQRTKDLLTQGSGTPR
                     ETYVSVYRHLRDSDDLLVHFFDDWRRSNLLFKMAGLREYGLLTEAHIQMLSPLAQEKL
                     NAIKELRAHMRATKGTPSKHRKKRGDSR"
     gene            complement(12973..13506)
                     /locus_tag="PKI64_05275"
     CDS             complement(12973..13506)
                     /locus_tag="PKI64_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00457.1"
                     /translation="MNSRLSILLMLAAPLVAYGQTDADIERKMKGIIVPIIEFRQASA
                     TDVLDFLVEATAATDPDKYSIALINTNPPTTREYYTYEVEDGTPVEFHPLTLEYKRTS
                     LYDAMNQITRECGLTYQIENGTIHYFKDGKRVIRKKHVEQVIPSHGTPGALDTEWGFG
                     GTDEAFDPEWGIPFGEP"
     gene            complement(13594..14259)
                     /locus_tag="PKI64_05280"
     CDS             complement(13594..14259)
                     /locus_tag="PKI64_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE00458.1"
                     /translation="MMPRHSAPRRLAGFTFVELLLALALGALVVTILGVLLRGLFVAN
                     EHQAGRLRGAPAARAALRQMNREIACAFAPPVEKLAPLELTTSTEPDKPQVALSFYAP
                     VPAPLEYDLAHITYEVVRAGGDRFALRRLAAPCSGPYTNAPVTNLLYAGRFALEIAVF
                     TNDTPHTAWPLPQDKEQPALPKSVRLTLTLPGQPPLATETLIQTMLPVRGPHASQEPK
                     AGD"
     gene            complement(14286..14780)
                     /locus_tag="PKI64_05285"
     CDS             complement(14286..14780)
                     /locus_tag="PKI64_05285"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE00459.1"
                     /translation="MAKLSGMSGEFKGREFPIEQGEITIGRKADNTILLDHPTISSHH
                     CRIVRSGDSCVLEDLDSTNGTRVNSRDVREAILHHKDLIQLGSIEFLFDAPELAAASA
                     RYTDANAEMATGAMSAPQDFASISPFGAPPKERRGFWYVVLAIVGVIALASLLTFIFM
                     LINA"
     gene            complement(14889..16628)
                     /locus_tag="PKI64_05290"
     CDS             complement(14889..16628)
                     /locus_tag="PKI64_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOE00460.1"
                     /translation="MLHLLPTPAVVEYTAGSFVLPAMPRVCIPVQCDARVSRVLHELF
                     PGLHRICCRETQPDVRLETATGPAESYELRITPAGLRIAAPDAAGFFYALQTLRQIMA
                     QAGATLPGLRVADAPVFARRGYYLDISRGRVPRLDMLRRRIQLLASLKINHLQLYFEH
                     PFRFQFDPDIAGDGDAYDAADLRALDVFCREHFIEFTPSFTCFGHLGRILSLPAYRHL
                     AEVEFPAPSWVAATWLQRLRGATLYSRDPAAQDLLRRILDEFLPCFSSPRFNLCGDET
                     HDLGKRHPHATPADLARQYEEHVRFVQSVAARHGKSIMLWGDMLLQHPAALAELPADC
                     EILDWAYFPTNRFEKCGEFTARGLATTVCPSVRGFGLMFNAVAEARDVIAAYARTGHQ
                     HGARGLLNTDWGDYGHFNMPPAALHGLALGAQLAWNPNNDAHAAFDRAFSRVLFNAPD
                     SRPAELFTLAGSVPPVVAAWPFAPLRGLPRPADPAPLREIAAQAEAWAAEFAALPASP
                     WTDETDLAQLALACRFLRLGALLAADAPAAETRPLLDELEKAYTPLWFAESLPRGLLD
                     LHHRGFAPLRARL"
     gene            complement(16892..17743)
                     /locus_tag="PKI64_05295"
     CDS             complement(16892..17743)
                     /locus_tag="PKI64_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE00461.1"
                     /translation="MNLQNMYDLFDYVVFSVRGRSLTLGDLLWALLLLAGGWVVARVA
                     AWLVRRKMAPRFGFSPRTSDLAPRWTFFLLWSLFILLGLRVLGIPLTFLNFLGGAIAL
                     GFGFGAQTLCSNLISGVIILIARPYRVGDIIEVDGQAGTVIAVGMRATEVLTYDGVNL
                     VVPNASFLANTIVNRTNYERALRGLVTITAAYSADSRRVDELLRTTAAAHPAVINKPG
                     RAPWVIFDNFGSNGLEFKLYFWVDTTRGSVAATASEIRHNVLTVFRAEGLAIPLTPHI
                     LNRPPAT"
BASE COUNT         3161 a         5330 c         6182 g         3290 t
ORIGIN      
        1 tgctcatgat aagcatgtta catagctgtc ttgtctaaac gttgggacgc gactgcatca
       61 ttataataaa aaagaaatgc agctattggc acagcaagcg tcgtaagctg gctgtttttt
      121 ggaggtacac cttcttgagc aggctcaagg gtaggggggc aatgagcaca atccgggagc
      181 ctgttccccc ttggctgttc ctgtagccgg tctcgctgag gccggcggct gttcacggct
      241 gccgtgggct tgggaattga ttttttcatg caaaacacat ccaccccacc gagaacggct
      301 acaggaagaa catcctgcgt ttctgctagt ttcagggacg aaaagcgcaa aaaaccttca
      361 aaaacagcct gtttttcttg tttttggcca ttttggggct gtttctgtag ccggtctcgg
      421 tgaggccggc ggctgttcac ggcttccacc agcttgagtc tcccggcccc aaaaaccgct
      481 tccggttgga ttacagggct ggaatggttg cagaccttcc agcctttccc ggcgggaagg
      541 cttcgttcct caatcattcc tgtcatgcaa agaccgaaaa agggaggggt aggggcaggc
      601 ggggggcgaa aaataaagaa gaggggattg acaggcgggg cggtattatg taatacgtaa
      661 tacatgatgc ggagtagtcc atgatcgagg gttggatcac ccagttgcgc aagggcgtgc
      721 tggagtattg cgtgctgctg gtgctgcgcc gaggggagag ctatggctat gagctggtgc
      781 aggcgctgca agccagcgac gacttggcgg tgtcggaaag cacggtgtat cccatcttgg
      841 cgcggctgaa gcaggagggg tacctgaagg cgcgcgatgt gccgtcggcg gcggggccgc
      901 cgcggcgata tttttcgctg acgccgcgcg gcaaggtgcg gttgtcggaa atgaatgcct
      961 attggccggt gttgatgagt gcgttggaga aattgaaagc gaaatagggg aaggaacccg
     1021 gaagatgaag tggagcatgg aagcaaaaca gcgggtggag gattacctgc acgaggtgga
     1081 acggcatttg gcgcacaagc cggagagcgt acgcaaggat gtcgtggagg ggttgcgcaa
     1141 tcagatttcg gaatcggttc agcggttgga gatcatcggc ggggtcatcg ggccggaggt
     1201 gattgaacag attctggcgg ggatggattc gccggagtcg tttgcggagg cggcgctgga
     1261 ggtggtcgga gcagcggcca cggaactggc gccgacagcg attcggagcg ggggggggcg
     1321 gtggttcgcg ctggcgctgg cgtttctggc gatcaatgcc tacggcgttt ggaaatggac
     1381 gcggactctt gcgccaccga gtccggcgcc cacagcggaa acggcggcga taccgcccgc
     1441 cccgaagccc attgagcggg tactccggtt gcgccaaatt ggccaagtgg atgtgtcggc
     1501 gcaacgcgaa gtgacgttga agctggcgtt caacgcggct ccggaccgta cgcaactgac
     1561 gcggttcttg cgcctgacgg cggaggggca aggggaagtg aaatactggc tgctgggcgg
     1621 agtgaacgcg gaggaggcgg tcattgaaac cgagccggtg ctggcggaaa agctggagta
     1681 ccgcttggag cccggtctgc cggccaagag tgaggatatc cagccggaag atcgcctgcg
     1741 cgagggttcg ttacgtatgg aaatgaatct gcaactgcgg gaggtggagg cgcgttcacc
     1801 atcgttcgaa cagccgtaca tttcggctga gttcaacgca gtgcctaacg cggagaagtt
     1861 ggcggagtat gtgagcgtca agccggcggt ggatttcacg gtggaaatca tgaggcggtg
     1921 gtgggagacc gggatgcgca tccgtggcga tttccggccg ggtacgattt atgacgtgac
     1981 cttcaaaaag ggcctgccgg cggccaatgg gtccagtctg gcccaagcgc tgacccggtc
     2041 cgtgcagatt ccggagccgg ggcctgccgt aaaattggac aataacggtt attatctttc
     2101 gccgcgcggc acattgcgcg taccggtggc ggcggtgaat ctcaaggaat atgtggcccg
     2161 gctgcaaccg gtgtttatca acaacctcgt gccgttgacg ctggccgatc gagaacaagt
     2221 atatttccgc aatgatcagg atctgtatgg cgaggcccag gcgctgacca accgcctgcc
     2281 ggccgcggcg ccgggagaaa ttgtccgcac gagtctcgac ctgcgcgcgc tggcgggcgg
     2341 cgagttgcgc ggagcgtatg ccttgcgcgt ggaagcggac tccgaccgcg gcgaggaccg
     2401 gatcattgtg gtgacggacc tcggcattgc caccaagctg catgatggcg gcgcgctggt
     2461 gtgggtcaat tccctgcgcg atgcccagcc ggtggccggc gccacggtga ccgttttggc
     2521 acgcaataac caacctctcg cccgtggcga aacagacggg caggggcttc tgcggttgga
     2581 gtgggcgcaa accgataccg cccggccgtg cattgtggtg gcggaacgcg acggcgacct
     2641 gtcctacatc aatttggagc gcacccggaa ctatccgggc gaggggacca gcggcgcggc
     2701 gtacctggcc gacggcaaac tcgaggcggc tgttttcacg gagcgcggca tctaccgccc
     2761 cggcgaaacc gtgttcatgc aggcgctggt gcgcgacaac caactgcgcg cacccgaggc
     2821 gatccccgtg ctgtggctgg tggtgcagcc cgatggccgc gtgtaccgcg agattcccgc
     2881 cacattggat gcctttggct ccgcgcaggc cacggtggtt ttgccggatt acctaaagac
     2941 cgggcggtac tccatccagt tggccatgcc gggcacgcgc acggttctgg ggagtaccaa
     3001 ggtgtcgctg gaagattttg tgccaccgca aatccgggtc aaggtgacag cgccagaggg
     3061 gcgtcacacg gcggggagtg cgctgggttc gcgcgtgcgc gccgagcatc tctttggtcg
     3121 cccggcagtc ggcctcaagg ccgagggctg ggccgttttc acgccggtgg aatttgcgcc
     3181 gacgaactgg cccggctgga cgtttggcga tccggagcgg cagttcgatg gcgaagagta
     3241 cgagcagggg gtgcagaatc tggatgatga cggcgtcgcg gagtttgagg ccgtcagccg
     3301 caaggcatgg cgcccacccg cagctttgca gttggtgcag caagtgaccg tgacggaagc
     3361 caacgggcgc ggcgtgacgg cgtacgaccg cacgctgatt gatccctacc cattctatct
     3421 cggcttgcag ccggcgtggg aggggggcgc ggtgcgcgtg gggcaaacgc agcgggtggc
     3481 cgtggtggaa gtgcagccgg acggcaccgg cgtggccaag ggcaagccgg tggcgctggc
     3541 gctggcgcgc gtgtcgtgga actcggtgtt gcgccggaac gacaacggtc gctacgaatg
     3601 gaaatcggaa aaacagacgg tggtgatccg gcgcgacacg ctggccgccg gcggcacgcc
     3661 gcaggattgg acgttcgcgg tggacaccgc cggtgactat gagctggtgg ccaccgagct
     3721 agcgtcgggc gcgcaaacgc ggctcagctt ttttgccgga tcgccggagc aggaatggca
     3781 ggcttggagc cgcgaaaaac ccggccgcgt tgagctggcg tgggacaaga aaacctaccg
     3841 gcccggcgat accgcccggc tgcaagtccg cgccccgttt gccggccccg ccctgctcac
     3901 ggtggaaacc gaccgcgtgc gcgaggcgcg cgtggtcatg ttggagaaaa atacggcgga
     3961 gttcgaggtg cccgtgaccg ccgcgcaggt gcccaatgcg tggtgtacga tgacgctgat
     4021 tcggcccgcg caggcggagg cggtgtggag cgcgcaccgc gccagcggcg ttattgcgct
     4081 ggtggtggac ccgcccgaac cgcatttggc ggtgacggtg gacgcgccgg cgcgcgtgtt
     4141 gccgcaaacc ccgctgcaag ccaccatcac cgtgcgcgat catgccggcc aaccggcgca
     4201 gggcgcggtg accgtgatgg ccgtggatga agccatctgc atgctgacgg attttgccac
     4261 gcccgatccg gccgcggaat ttttgcggtg ccgcggcctc ggtgtgaatg actatgacct
     4321 ctacgccgaa ctgatgccgg tgacggaaga tgctgtggaa accgtgccgc gcgcgggcgg
     4381 cggcgccggg gcggagttgc gccgccggtt gaatcctatc aaggccaacc gcttcaagcc
     4441 gctggccctc tggcaggccg atctgccact ggatggcgat ggccgcgcca ccgcgcagtt
     4501 tgatttgccc gaattcaatg gcgaagtgcg cctgatggtt gtggcctata acgccgcgca
     4561 aacgggcgcg acctccatgc cggtcaaggt caagcgtgac ctcgtggtac agtcggcgtt
     4621 gcctcgtttc atggccattg gcgaccgcag cgatgccggc gttgtgttat acaacgaaag
     4681 cggcgcgccg ttgacggcca ccgtgcgcgc gctgtgcggt gggccgctgc gggcggaaaa
     4741 tgcggagctg accgttgcgc tgccaacggg tggatccacg aatctggtgg tgccgctggt
     4801 ggctggcccc ggcccaggtg tggcgcgctt ctcgttggaa gtcagcggtg gcggcgaaga
     4861 gttccgcgat tcgattgagc tggcggtgcg accggcatcc ggcacgcgca gcctgtcggt
     4921 gcagcgcgtg gtggcgcccg gcgaagaggc cgcgttcacc gcgcctgccg attggttgcc
     4981 ggcttcggtg gcgctgacgg gcgtgctttc cgcgctgcca tcgctggaga tgggccgcgc
     5041 gctggattac gtggtgaact atccgtacgg ctgcctcgag caaaccgtct ccggagcgtt
     5101 cccgctgctc tacgccggcg agtggacgca gcggatgctg cccagtgcga ccgcagccgg
     5161 cgacatcccc ggcatggtgg atgctgccat ccgccgtgtg ttgaacatgc agcagaacaa
     5221 cggcgggttt gctgtctggc cattcacatc ggatgtggaa gaagacgtaa gcctctacgc
     5281 cacgcatttt ctggtggaag cccaagcggc gggcttcacg gtgcccgccg accgcgtgca
     5341 agccgcgcta aaccggctga agaattggct cgaccgctcc atcccggcca aggccacgcc
     5401 ggagcaaatg gctgacgacc tgcaaacacg cgcctatatt tgccatattc tcgcccgcgc
     5461 tggccaaccg cacgaaggtt ggaacgcgcg cctccgcgaa caggccgatc agctcaactt
     5521 tgccgcgcgc gcccacacag catccgcgct gctgttggcg ggcgagccac ggcaggcgct
     5581 cccgctgctg gaacaaacca agttgccgac ctcccggccc cacctggccg gcgactggcg
     5641 cgccagcgat attcgcgagg ctgcgctcat gttgtccgcg tggctggatg tggacccgga
     5701 aaacgaagcc gttgcccggc tcgcgcaata tctgcgcaac cggcagcaga acggccattg
     5761 gggcaatacg caggataacg ccatggcgct gctggcgttt ggcaaactgg cgcgcgcgct
     5821 gccggttaag gagcagccgt ttgccggccg cttcacagtg gcggatcaca cgcagacgtt
     5881 cggcacgacc aatgatgtcc agtacaccag cgccccgggc gagggcggca cgctcacggt
     5941 gcataacgac ggtcccggca aaatgtacgc gtgggtgcag tttaccggcg tgtccaccga
     6001 gccggagccg ccgcacacca accaactggc gctctcgcgc gagtttttgg accggcaggg
     6061 cgagccgttg gcggtgaatt cgctcccgca gggcgatttg ttcgtggtga aaatcacggt
     6121 ggacccgcgc ggccaaccgc tggaccacgt ggtgatcgaa gacctcctgc ccgccggctg
     6181 ggaaattgaa aatcccaacc tgcagaccgc ccaaaacctg ccgtggctca aggacaaggg
     6241 cgacaagccc cgccacaccg aggcgcgcga tgaccggatg ctgatcttta gcgggcggat
     6301 gcaaaaaccc gccacttact actactccgt gcgcgcggtc acgccgggcg aatacaccct
     6361 gcccgcgctg actgccgtcg ccatgtatga tccggaaatc agcgcggcca ccgccagcag
     6421 caaggtacgc gtcgtgccgt gacctcccgc cgggtcaaat ggctgcgccg ttccgccttt
     6481 gcggtggcgg cgctgaccgc cggcagctat gccgcggtgt gctccgtgcc gttccccgaa
     6541 gcggatttgg cgcgcctgcc ggcggccgtg gtgctggcgg atcgcacggg cgagccgctg
     6601 cgcctcaaac tggccgcggg cggttacgat tgtcgtcccg gctacgcgcc cgatcccgcg
     6661 cactggatcg gcaaagccat cgtggccgcc gaagaccagc gcttctggcg gcatcccggc
     6721 gtggatgttt tggcgctggc gcgcgcggtg gccgacaacg tcgcgctggg ccggcgtgtg
     6781 tccggcgcat ccaccatttc cacgcaggtc atccggttgg cccagccgcg ccaacgcacg
     6841 ctggggacca agtgcatcga agccttccgc gcgctgcaac tggagcggcg ctacgacaaa
     6901 cagaccatcc tggggatgta tctcgaccgc gcgccgtttg gcggcaactt ggtgggcatt
     6961 gaggccgccg cccgccacta ctttgccaag cgtccggcgg acttaagtct cgccgaagcc
     7021 gcgctgctgg cgggattgcc gcagtcgccc tcgcgcctgc gtcccgaccg tcatctggcg
     7081 cgcgcccgtc aacggcaggc gtatgtgctg gaacgcatgg aggcgtgcgg catgatcacg
     7141 gcggatgaac gcgccgcggc acaagcccag ccgctggcct tccagcgcgc gccgcggcct
     7201 ttcgccgcgc cgcacttttg cgaaatggtc ggcgtgcccg cccgcacagc cgacggcgcc
     7261 cctctgcgca ccacgctgga tttgcgccag cagcggctgg ccgaggatgt cctcgcgcgg
     7321 catctgcagc gcagccccat caacagcggc gccattgtgc tgctggaggt gcgcactggc
     7381 gcggtgcgcg cgctggtggg ctcgccggat tacttcgcgc gcgagaccgc ggggcaggtc
     7441 aacggcgcca cggcccgtcg cgccgccggc tccactttga agccgtttgc ctacgcgctg
     7501 gcgcttgatc gcggattcat cacgccgggc atgatgatcc ccgacattcc cacccgctat
     7561 cgcgatctcg acccacgcaa cttttcgccg gagtatcgcg ggctggtcag cgtgcgcgac
     7621 gccctgatgc tgtcgctcaa cctgccggcc atcgaggtgg aaaagcgcgt gggccaggag
     7681 cggttctgga gcacgctgcg cgcgctgggc ctcggcacca ttgaccagcc cgccgcgcac
     7741 tacggcctcg ggctggtgct gggcaacacc gaagtgcgcc tgcttgatct ggccaacgcc
     7801 tacgcctgtc tcgcgcgcgg cggtgtctgg ttcccgccgc gcctgctgga aaactcccgc
     7861 ccgcccgcgg ggcgcgaact tttttcgccg gaagcctgct ggctggtggc ggagatgctc
     7921 tccggcgagg agcgcaccat ggacattgcc ggccacgcgg cggatgtgcg tttgccgccc
     7981 atggcgtgga aaactggcac ctccgctggc ttgcgcgacg cgtggaccgt ggcgtggaat
     8041 cccgactacg tgattggcgt atggctgggc aacgccgatg gctcacccgc cgatgaactc
     8101 gtggggcggc tcagcgccac acccatcgct tgggaagttt tccgtcagct ttaccccgac
     8161 aacaacggtc cttggtacgc gcgcccaccc ggtatcatcg agcgcaacgt gtgcgcggtt
     8221 agcggttgcg ttcctggtgc gaactgcccg cacgccgtgc aggattgggc catcgccaag
     8281 gtcacccgcc atgagccctg ccccgttcac cggcaaggcc cagccgagac ttggccggtg
     8341 gcggtggcgt cgttcctcgc gcggcgcacg gcgcccgccg cggatgaatc cgctgccgcg
     8401 ctgcgcatcc gctcccccgc ccgcggcagc acctaccgct ggcttcccga tttgacggtg
     8461 gatgcccaac gcctcgccct cgatgccgcc agcgatcgcg ccggcgaaac tctccattgg
     8521 ttcgtcaacg atcagcccat cggccgctcc cgcgccggcg agccgctttt ctggccgctc
     8581 acccccggca cgcacaccat cgtgtgcagc accgcgcgcg gcctgagcga ccgcattcaa
     8641 atcgcggtgg aaacccaata gcgcgggcgc gggattgatt gccgcgccgc cagccgctag
     8701 gatgcgcgca tgaattggtc cctgcccaac ggccccgcat gaagatcagc atcgtcaccg
     8761 caaactataa tggcgggcgt tggctggcgc gctgcatgga tagcgtgctg aaccaggaat
     8821 tggcgccggg cgatgaattg gaatacattc ttattgatgg ggccagcacg gatgactcgc
     8881 tgcaaattgc ccaagcccgc cgcggacggc tggccgcgct catcagcgaa aaagaccacg
     8941 gcccggcgga tgcactcaac aagggattcg cactggccac cggcgacctg ctcggctggc
     9001 taaatgccga cgatgtctat gagccgggcg cgttggcgcg cgcgctggcc gcggccaagg
     9061 cgcgcccgca agccgcgttc ttttttggca aatgccggat tgtggatgaa gcggggcgcg
     9121 atatccgcag ctttcccacg ctggtgaaaa atgcgtgcat gccgttttcc agccgctttc
     9181 tcatccagtg catcaactat gtctcgcagc cggcctcgtg gttccggtgc gcggcactgg
     9241 cagcggcggg gggcttgcgg ctggatttca aggccgcgtg ggattatgac ctcacgctgc
     9301 gcctctggcg gcagggcggg ggggtgcgga ttcccgggcc gccactggcg gcgttccagt
     9361 ggcatccggg ttcgatcagc ggcacgcact acgagcagca attcgccgag gaatggcgcg
     9421 ccgcggtggc ggatgccgga cgcttctcgc cgcaggcgtt ggtgcattgg ttcgtgaggc
     9481 acggcatcgt gctgtgctac cggctgatga accgcccgcg cgcctgaaaa gtgagcgcgg
     9541 gggcgggggg gcgggctact tggccttgag gatcagcgtg ggcggacgga agcgttggct
     9601 atcctcggtg gcggtgatcc accaaccctt cttgtggtcg ccatcggagc cggcggtcag
     9661 caccagcgtc acttcatcgc cgggaaactc gcgcaggaat ttggtgagcg cgcggtcttc
     9721 cacggtcagg atatcgccgg tctgcggatt cggcggtaga ggataattgg ccaacaacgt
     9781 agcctgggtg ggcatcagat taccggtgga ggcgggatcg ttgccgggag ccgtttgcca
     9841 cgtgatggat tcatcccaga cggccgcggt cttcagaccc cacacttgga gtgtatagga
     9901 tttggttttg ggtttaaccg gcgtggtttt gctgaacgtc aggcgcagga cggcctggtc
     9961 cagccgcgcc ttgtccacgc cggtgagcgg aaagcgcaga tatattttgc tggcttcggc
    10021 cagatcgccg gtgccgccgt gcaacggcag aatgttctgg accttgaagt cgccggatga
    10081 gttgtcgccg ctgatggaaa catccatgct ggcggggatg gtccaatcgt tgattggcgc
    10141 ggcgggcgcg gccgcgccgc cggcggcggg agcggcggta tccacccgca cccataccag
    10201 ttggccaccg cgcatgacct gtttaagccg accggcggcg atgtccgcct gccgctgagc
    10261 ttcttcctgc gcttgcttgc gtttgtcttt atagatggac ttgcccacca ccgtcagccc
    10321 ggcaatcagc gccaccccca caatgcccag caccacaggc acgaccagat tgcgccgcgc
    10381 ggcttcgggc tttttgccgg actgcgccgc cgtcagcggt tcgagcgcgt cgttgatgtc
    10441 cttgagcagc gactcatacg ttggatagcg cgcgaaggaa tccatttcca gcatgcgcag
    10501 caaaatggcc gccgtgcgcg cgctgatgtt gtttttgaac acgcgcggat cgggcgcggg
    10561 ttccttgaag cgcagcagca cggtatcggt gacggtttcg caattgaagg gcggctcgcc
    10621 ggtgagcacg tgatagagcg taccgcccag cgaatagata tcggagcccg cgttgggcgc
    10681 ctgccccttc accacttccg gcgccacata gtacggggtg ccccacactt cgccgggttg
    10741 cggcttttgg cccttgaagc gggcaaggcc gaagtccgcc accttggcgt tccccgcgcg
    10801 gtcgaagagg atgttggccg gcttgatgtc gccgtgggtc atgccggcgg cgttggccgc
    10861 ctgcaggccg cggatgatct gcgtggccat gcgcagcacg aaagtttcat cgagctgctt
    10921 tttcttttcc tgcatgtggt cgagccgccc gccatccacc agctccatca cgatgtacgg
    10981 ctggccgttt tcctcgccgt agctgtaaat ctgcacaatg tgtgggttgt tgatggccgc
    11041 cagcgcgcgg gcctcctgct gcaattgcgc cacgaacgcg gggtcttggc cgatggcctg
    11101 ctgcatcacc ttgagcgcca ccgtgcgccc gagggtttcg tcataggcct tatagaccgc
    11161 gcccatgccg cccttgccca actgctccag caggatgaag ttggcaaagc gcgccggcac
    11221 gcgcatttcc gcctggcaaa ccgggcagtt cagcagcgag aacggggcgg cttggctggt
    11281 gtcaatgggc tggttgcact tggagcaggc gataacatca aatcgctcaa tcaggatatc
    11341 ccctgccagt tcgttgccca tgcgtttgcc tcaaacgcca atcctgccag cctcgcgcag
    11401 cagaagcaac atcaaagcct tgtgcatgtg catccggttt tcggcttggt cgaagaccac
    11461 ggactgcggg ccgtcaatga cctcggcggt ttgctccatg ccgcgcatag ccggcaagca
    11521 gttcatgaag atcgccgaag gcttggccag cttcatcagc tcgctggtca cccggtacgg
    11581 cgcaaaaatt tgcatccgtt cgccggcctt ttccttgggg atgtgatagc tcatccacga
    11641 atcggtatag accacatcgg catcgcgcac agcggcttgg gcatcgtgac cccaatcgaa
    11701 gcgcgtgccg gtgcgggcgg catcggcctc ggccgccgcg cgggcggtgg cggcgggctc
    11761 atagtccgcg ccggtagggc aggctacggc aaggtgcatg cccatcttgg gaacggcaaa
    11821 cagcagcgag tgcgtcacat tgttgaagct gtcgcccaga tacgccagtt tcagccccgc
    11881 cagtttgccc agatgctcct ccacggtttg caggtccgcc aggatttggc ccggatggga
    11941 aaagttcgtc agcgcgttga tgatgggtac cgtggtgtac cgcgccattt ccagcaggtc
    12001 ctcgtgcttg aacagccgcg ccatgatgat gtccacatag cgacaggtcg tgcgcaccat
    12061 gtcggcaatg gattccttgc cgctgccgaa gggggaggtg gacgtgtcgt aataaattgc
    12121 gtggccgccc atttggttca tgcccgcctc gaacgagagc cgggtgcgca gcgatggctt
    12181 ctcaaagaac atgcagagcg tcttgcgctt gagcgaatcc gcgtaatccg cggggtgggc
    12241 cttgatggtg cgcgccagcg caatcacctc ggcaatctgg tcctggttcc aatcggccaa
    12301 cgtcaataaa tgtctcatcg gggtcacact ttctgaaaaa agtttgcgcg gtatcctagc
    12361 gcacccccgc cccccatgaa aaactttttc taaaacaatg gttcatccca aaaatcctgc
    12421 ccatcccccc caaaaaaact cagcggctat cgccacgctt cttgcggtgc ttggaaggtg
    12481 tgcccttggt ggcacgcatg tgcgcgcgta attctttgat cgcattcaat ttttcttggg
    12541 cgaggggcga tagcatttgg atatgcgcct cggtaagcaa cccatattcc cgcagcccgg
    12601 ccatcttgaa caataggttg gaacgccgcc agtcgtcgaa gaaatggacg agcagatcat
    12661 ccgaatcccg cagatggcga tacacgctca catacgtttc gcgtggagtg ccgcttcctt
    12721 gggtcaggag gtccttggtt cgctggttaa tctgctcata cagcgtgttc agcagctcgc
    12781 cctgaatctt gcacatgtac ttccagtctc gctccatgat tccgtgatcc atgatcttcc
    12841 tccctcattc tgatccgtct tggcgagagt ccttgtttcc ttgcagcgac aacggcattc
    12901 atccaaaacc gaaatgttaa ttgtcaaggg ctgaccccga cgcgccgagt tcatttctgg
    12961 tcaggcccaa ttctatggtt caccgaacgg tatgccccac tcggggtcga acgcttcatc
    13021 ggtgccgccg aaaccccact ccgtatctaa cgcacccggc gtgccgtgcg atgggatcac
    13081 ttgttcgacg tgtttcttcc ggatgactct cttgccgtct ttgaagtaat ggatggtgcc
    13141 gttttcaatt tggtaagtaa gcccgcactc tctggttatc tgattcatag cgtcgtacag
    13201 tgatgtgcgt ttgtattcca atgtcagggg atgaaattcc acgggggtgc cgtcctcaac
    13261 ctcatatgta tagtattccc tggttgtggg cgggttggta tttataaggg cgatgctata
    13321 cttatctggg tctgtggcag cagtagcttc aactagaaaa tcaagcacat cggttgcact
    13381 ggcctgccga aattcaatga tgggaacaat gatacccttc attttccgct cgatatccgc
    13441 gtctgtttgg ccataggcga cgagcggggc ggctagcatt agtaaaatac tcaggcgact
    13501 attcattttc tttcattgaa cagttttgtt ccccctctcc agtctctagt ctccagtctc
    13561 aagtctctcg cccccgcctt ccgcctcgag tcttcagtct ccggccttgg gctcttggct
    13621 ggcgtggggg ccgcggacgg ggagcatggt ctggatgagg gtttcggtgg cgaggggggg
    13681 ctggccgggg agggtgaggg tcaagcgcac gctcttgggc agcgcgggtt gctccttgtc
    13741 ctggggcagg ggccacgcgg tgtgcggggt gtcgttggtg aatacggcga tttccagcgc
    13801 gaagcgcccg gcatacagca ggttggtgac gggcgcgttg gtgtacggcc cggagcaagg
    13861 cgcagccagc cggcgcagcg cgaaccggtc gccgccggcg cggaccactt cgtaggtgat
    13921 gtgcgccaag tcatattcca gcggggcggg cacgggggcg taaaacgaga gggccacctg
    13981 cggcttgtcc ggttcggtgg aggtggttag ttccagcggt gccagctttt ccacgggggg
    14041 ggcgaaggca caggcgattt cgcggttcat ttggcgcagc gctgcgcgcg cggcaggggc
    14101 gccacgcagc cggccggcct gatgttcgtt ggccacaaaa agcccgcgca gcagtacgcc
    14161 gagaatggtg accaccagtg cgcccagcgc cagcgccagc agcagctcca caaatgtgaa
    14221 ccccgccaat cggcgcggcg ctgaatggcg gggcatcatg acggcccggg ggtggcgggc
    14281 gcggcctagg cgttgattag catgaagatg aacgtcagca gcgacgccag tgcaatcacg
    14341 ccgacgatgg ccagcacgac ataccagaac ccgcggcgct ccttgggcgg cgcgccgaag
    14401 ggcgagatgc tggcgaagtc ctgcggtgcg ctcatggcgc cggtggccat ttccgcgttg
    14461 gcatcggtat agcgcgcgct ggccgcggcc agttcggggg cgtcgaagag aaactcgatg
    14521 gagcccagtt ggatcaggtc cttgtggtgc aggatggcct cgcggacatc gcgcgagttg
    14581 acgcgggtgc cgttggtgga gtccaaatct tccagcacgc aggagtcgcc gctgcgcacg
    14641 atgcggcaat ggtggctgga aatggtgggg tggtccaaca ggatggtgtt gtccgccttg
    14701 cgtccaatgg tgatttcgcc ctgttcaata ggaaactcgc gtcctttgaa ctctccggac
    14761 atcccgctta acttggccat gaacccgcct ccatctcctg ctgtagtgca ctaaccatct
    14821 gcaagcgggc atcgtgccga ttcccgggcg gaaaagcaat cgcaaatccg cggggattgg
    14881 gcagcgggtc acaaacgggc gcgcaggggg gcgaagccgc ggtggtgcag atcgagcagg
    14941 ccgcgcggca aactctcggc gaaccagagg ggggtgtaag ctttttcgag ttcgtcgagc
    15001 agcgggcggg tttcggcggc gggggcgtcg gcggcgagca gcgcgccaag gcgcaggaag
    15061 cggcaggcca gcgcgagctg ggccagatcg gtttcgtcgg tccaggggct ggcgggtagc
    15121 gcggcgaact cggcggccca cgcctcggct tgcgcggcga tttcgcgcag gggggcggga
    15181 tcggcggggc ggggcaggcc gcgcagcggg gcaaacggcc acgcggccac cacgggcggc
    15241 acggagccgg caagggtaaa caactcggcg gggcgggagt cgggtgcatt gaatagcaca
    15301 cgggagaagg cgcggtcgaa cgcggcgtgc gcgtcgttgt tcggattcca cgcgagctgc
    15361 gcgccgaggg cgaggccgtg cagggcggcg gggggcatgt tgaagtggcc gtagtcgccc
    15421 cagtcggtgt tgagcaggcc gcgcgcgccg tgctggtggc cggtgcgggc gtaggcggcg
    15481 atgacgtcgc gggcctcggc cacggcgttg aacatcaggc caaagccgcg cacggagggg
    15541 cagacggtgg tggcgaggcc gcgcgcggtg aactcgccgc atttctcgaa gcggttggtg
    15601 gggaagtagg cccagtcgag gatttcgcaa tcggcgggca gctcggcgag ggcggcgggg
    15661 tgctggagaa gcatgtcgcc ccagagcatg atggatttgc catggcgggc ggcgactgat
    15721 tgcacaaagc ggacgtgctc ctcatattgc cgcgcaaggt cggcgggggt ggcgtggggg
    15781 tggcgcttgc cgagatcgtg ggtttcatcg ccacagaggt tgaagcgggg ggaggaaaag
    15841 cacggcagga attcatcgag gatgcgccgc agcaaatctt gcgcggcggg gtcgcgggag
    15901 tagagcgtgg cgccgcgcag ccgctggagc cacgtggcgg cgacccacga gggcgcgggg
    15961 aattccacct cggcgaggtg gcggtaggcg ggcagcgaaa ggatgcgccc cagatgcccg
    16021 aagcaggtga acgagggcgt gaactcgatg aagtgctcgc ggcagaacac atccagcgcg
    16081 cgcaggtcgg cggcgtcgta ggcgtcgcca tcgccggcga tgtccggatc gaattggaag
    16141 cggaaggggt gctcaaaata taattgcaga tggttgatct tgaggctggc gagcagttgg
    16201 atgcggcggc ggagcatatc gaggcgcggc acgcgcccgc gactgatgtc gaggtagtag
    16261 ccgcggcggg cgaagaccgg cgcatcggcc acgcgcaggc cgggcagtgt cgcgccggct
    16321 tgcgccatga tttggcgcag ggtttgcagc gcataaaaaa agccggcggc atcaggcgcg
    16381 gcaatccgca ggccggcggg cgtgatgcgc agctcgtagg attccgccgg gccggtggcg
    16441 gtttcgaggc gcacgtcggg ttgggtttcg cggcagcaaa tgcggtgcag gccggggaac
    16501 agctcgtgca gcacgcggct gacgcgggca tcgcactgca ccgggatgca cacgcggggc
    16561 atggcgggca ggacaaagct cccggccgtg tactcaacaa ccgcgggcgt gggcagcagg
    16621 tgcagcatgg ctcaactttt ccacggtgtg gaaaatcccg cccacgcggg atgaactatt
    16681 tttccacagt gtggaaaaat atggggtgaa ttttccacag tgtggaaaac cggggccgaa
    16741 tttttccaca caatggaaaa acttttggcg atttttccac acaatggaaa aactttgggt
    16801 ggatttttcc acagtgtgga aaatcccgcc cgcgcgggat gaactttttt ccacagtgtg
    16861 gaaaagtgcg gggcgcgctg ggcggccggg gtcatgtggc gggcgggcgg ttgaggatgt
    16921 ggggggtgag ggggatggcg aggccctcag cgcggaagac ggtgaggacg ttgtggcgga
    16981 tttcgctggc ggtggcggcg acggagccgc gcgtggtgtc cacccagaag tagagtttga
    17041 attcgaggcc gttgctgccg aagttgtcga agatgaccca cggcgcgcgg ccgggtttgt
    17101 tgatgacggc cgggtgcgcg gcggcggtgg tgcgcaggag ctcgtccacg cggcggctgt
    17161 cggcgctgta ggcggcggtg atggtgacaa ggccgcgcaa cgcgcgttcg tagttggtgc
    17221 ggttgacgat ggtgttggcc aaaaagctgg cgttggggac cacgaggttg acaccgtcat
    17281 aggtgaggac ttccgtggcg cgcatgccca cggcaatgac cgtgccggct tggccgtcca
    17341 cttcgatgat gtcgcccacg cggtacgggc gggcaatcag aatgatgacg ccgctgatga
    17401 ggttactgca caaggtttgc gcgccgaagc cgaagcccaa cgcgatggca ccgcccaaaa
    17461 agttgaggaa ggtcagcggg atgcccagca cccgcaggcc cagcagaatg aagaggctcc
    17521 aaagcaggaa gaacgtccag cgcggggcga gatcgctggt gcgcggcgag aaaccgaagc
    17581 gcggggccat cttgcgccgc accagccacg ccgccacccg tgccaccacc cagccaccgg
    17641 ccagcagcag cagggcccag agcaggtcgc ccaaggtgag cgagcggccg cgtacggaga
    17701 aaaccacgta atcaaaaagg tcgtacatgt tttgcagatt catgccggca gtgtagcccg
    17761 cgcgaaaacg gaaggcaaat ccatccgcgg cgccgcccgc cgccgcgagc agcggcagag
    17821 gtcagggttc agggttcagg gttcagggtt cagaaatttt ggcgggatgg cctaagtgct
    17881 ctcctggttg ttgccggttt tcagttcttg ctttcttggg tttccttata ggaactccag
    17941 agttatgaac aggcggggag gag
//
LOCUS       DAOPNP010000041        17930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3757_length_17930_cov_7.995804, whole genome shotgun sequence.
ACCESSION   DAOPNP010000041 DAOPNP010000000
VERSION     DAOPNP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17930
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3757_length_17930_cov_7.995804"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1322)
                     /locus_tag="PKI64_05300"
     CDS             complement(252..1322)
                     /locus_tag="PKI64_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00462.1"
                     /translation="MPHNIWHKLLAPDNQTFEIVLSEELDDVISILVDAQIDPKTFGG
                     NLCELLSEGLPSFSSIFDLEQRSLPTSPSVKKYTSVINRPVAVPLWFIAAFPDVLHWL
                     NERQAKIHRCASSEDTSVKEFDELLGLNMNQADDVDISQYQNRKGQLRSQYYRNLVHN
                     YLKRTGARLEVAVVPGSETDMYTAMDMLHAVGEESTFTLIATMTTKYYSELRNNYSAS
                     FPDETSLLAMAGILDANMSIFVTQQITIEQVIDLARRSGGDNRYFDFLISLSTLLIWV
                     DAQEMDPSDITAAGLEQADKIQESIRYALNTYKSEPMISQTARMVMRAPQFAYVRQAV
                     GVTSNSLFSKIKKLFGQQRMVQ"
     gene            complement(2008..2235)
                     /locus_tag="PKI64_05305"
     CDS             complement(2008..2235)
                     /locus_tag="PKI64_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOE00463.1"
                     /translation="MSRLPTLKGTELIRILGKFGFSVIRIKGSHHYLRHLDGRCTVVP
                     VHRGETIGRGLLAQILKDCELSRDDISPDGK"
     gene            complement(2232..2441)
                     /locus_tag="PKI64_05310"
     CDS             complement(2232..2441)
                     /locus_tag="PKI64_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE00464.1"
                     /translation="MREFNVVIERDADGYFVASVPSLKGCHTQAKSLDELVSRIKEAI
                     ELCLEVGDEPVNNEFIGVQRVSVMA"
     gene            complement(3106..3378)
                     /locus_tag="PKI64_05315"
     CDS             complement(3106..3378)
                     /locus_tag="PKI64_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00465.1"
                     /translation="MKKYLAPQALWPASVRPVSGKSPASTVRGYRGQEISPSRQGSRI
                     STQTAPKTQTGAKNEKYLASQALWPVSLRPAGFSGSSLFSVAEHGG"
     gene            complement(3696..4265)
                     /locus_tag="PKI64_05320"
     CDS             complement(3696..4265)
                     /locus_tag="PKI64_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00466.1"
                     /translation="MGKKNGEIEQELTGVVFYGWMERCNMRAARARDWSREELPAKRT
                     TIALDRLFSEEEMELIQRGVVPEQMEDKWFIYWEEGTLYFHRSWTGFCIYVVRFVLEN
                     RGWRMVRADVNRDPAEYNETSDKTDAVLVSALVDVLLLRREPALPQAGESDVQAAVLN
                     WSMIGRAMFGEHPRGLEDEERGGEDKPRR"
     gene            complement(4775..5344)
                     /locus_tag="PKI64_05325"
     CDS             complement(4775..5344)
                     /locus_tag="PKI64_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00467.1"
                     /translation="MRKLLPILQAAEQVVAILKQHNVDSVVIGAVALAAHHYVRHTQG
                     IDIGVSAALPVLQKVTESLNAAGLTAELSAPDADAPLGGVVNVSGGFGVVQIVNFCDR
                     FPAVIQDAIRDSTMLLRPGSALKLAPLPQLVALKLYAGGLKSKADILELLRHNPGADL
                     HDIMTTCQRYRLHGLEEIMAELARHGRNA"
     gene            complement(5349..5612)
                     /locus_tag="PKI64_05330"
     CDS             complement(5349..5612)
                     /locus_tag="PKI64_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00468.1"
                     /translation="MIGNKKEAMTSRRKITQGFKVVKDQEVGAASTQPGFPTMIPSVS
                     ADQSFARELARLQSLSIEARIEEALTMKEQFAWLNPVIGDTEP"
     gene            complement(6181..6324)
                     /locus_tag="PKI64_05335"
     CDS             complement(6181..6324)
                     /locus_tag="PKI64_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00469.1"
                     /translation="MELALTIAGWISVALLGWFIWQWLAGMVRGWRRTTTTITQNVNK
                     DLY"
     gene            complement(6414..7871)
                     /gene="gnd"
                     /locus_tag="PKI64_05340"
     CDS             complement(6414..7871)
                     /gene="gnd"
                     /locus_tag="PKI64_05340"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOE00470.1"
                     /translation="MKALADIGVVGLAVMGENLILNMENKGFTVACYNRTVAKVDAFL
                     GGRGKDKNLIGCHRPAELVRALARPRKIMMMVRAGKPVDDLIAELIPLLEPGDILIDG
                     GNSHYPDTIRRCAAAEANSLLYVGAGVSGGEEGALTGPSIMPGGHAAAWPHLKPIFQA
                     IAAKVDDGTPCCEWLGPDGSGHFVKMVHNGIEYGDMQLIAEAYHLLSVGLGMTPAEMG
                     AVFKQWNAGALDSYLIEITADILTQVDDETGRPLVDMILDAAGQKGTGKWTSQAGLDL
                     GVGIPQIAEAVFARCLSAIKEERVAAAAILPGPNGKITVDRAAFVADLEQAVHAAKIC
                     SYAQGYQLLRAASAEHGWNLNFGDIALLWRGGCIIRARFLGKIKEAFDAEPALANLLL
                     APYFRDVVGQTQAAWRRVVRAAAEAGIPLPAIGTALSYYDAYRSARLPANLLQAQRDY
                     FGAHTYERVDKPRGQFFHTNWTGRGGTTASTTYGV"
     gene            complement(7885..9360)
                     /gene="zwf"
                     /locus_tag="PKI64_05345"
     CDS             complement(7885..9360)
                     /gene="zwf"
                     /locus_tag="PKI64_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOE00471.1"
                     /translation="MSNEIPQAEATTIVIFGATGDLTQRKLIPALFHNRKKGRLWDRT
                     RIVGVARRDWSDDFFREHLREGVRRYGGPFDEKAWAEFAPRIRYVRGDLEVPEDYHEV
                     DRRLREIEGKPANRLYYLATAPEFYGPACDYLAAANMTAETEGWRRIIIEKPFGHDLA
                     SAVALNHRVHQSFREHQVYRIDHYLGKETAQNILFLRFANTLFEPVWNRRYISNIQIT
                     VAEDVDVGTRGGYYDQAGVVRDMFQNHLLQLLSLVTMEPPASLDANAVRNEKAKVLGN
                     IRAIRLEDAVRGQYEGYREAARVAPDSQTPTYAALKLYIDNWRWQGVPFYLRSGKAMA
                     ARGSSIIVEFQAPPDVMFGLARNQSFTPNMLSICIQPEEGVHLRFETKVPDTIAESRS
                     ANMDFSYRKAFPDIVLPDAYERLLLDAIKGDASLFARADGIEIAWRLIDPILAGWEEH
                     PERSPLHIYPRKSWGPAAADELLARDDHEWRLGCGEDGCWI"
     gene            complement(9353..10114)
                     /gene="pgl"
                     /locus_tag="PKI64_05350"
     CDS             complement(9353..10114)
                     /gene="pgl"
                     /locus_tag="PKI64_05350"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOE00472.1"
                     /translation="MNNPAHQFHVFRGPTELSPALARRVASAAATAVQERGRFRIALS
                     GGSAMLRLAAGFRLLPELNAYWGAWRVYWADERCVAADDPNSNYGAARAEFLDDVPIP
                     ADQIFPVDGTLLPEEAARAYEAALRREFAADGDAWPRFDVILLGVGGDGHTASLFPGN
                     PVLTEMVHWAAPVWQSPKPPPTRVTLTLPVLNHARQALFVAMGRDKARVVQKVRAVAN
                     GDLLPAQRVHLTAGPCEWFIDEQAAEQPTSESHHE"
     gene            complement(10164..11585)
                     /locus_tag="PKI64_05355"
     CDS             complement(10164..11585)
                     /locus_tag="PKI64_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE00473.1"
                     /translation="MKIARWLWVFAAWAAVVTRAAERAWDLPVMATWTEDPRTSVTLA
                     WERSAAEPAWVEYSTDGAIWQHVAQEQPVRRHVFTLRDLEPGTHYRYRLASWDGYQAE
                     GRFWTAPADKTTPFTFALHNDLQGGINVEAAKQVAQAVVRANPDFVLSTGDLGDSRYS
                     SDYANGVQSWQLFFDCTEPELAHFVFQVVTGNHDEPENPDSFWHRLFELPSHHDYVLD
                     VGPIRFVMLDSVEEQIPSRIAWLARELQGAAYDPEITWVIPAFHRPPFSYGEREGQEQ
                     VREWWVPLFTRYEADLVLNGHAHTYQRTKPLAGVNYLVSGGGGGRLYWVPEKHENLLF
                     ATSLYHFVQFNVAGDRIQLAATATDGTVFDRAEYKAHRHVRVEPIFPVRGEDCTVWYN
                     AAGGPLADAQQISVHVGRDNFADFLMDVPMEKDATTGLWKATFPTPSSAKWNVAFCFF
                     DPVNKVWHNNYQQDWQALVAREW"
     gene            11682..12362
                     /locus_tag="PKI64_05360"
     CDS             11682..12362
                     /locus_tag="PKI64_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00474.1"
                     /translation="MSASPQEFDFWYAVNNTEVVMPPRGRLETFGSTLIHYWLVTELM
                     DAIGQVRIREGRIQSFRPEILTPQSFSESPLEGFQEEQAEDFIRWLREHESDFMLLKY
                     GFRIRHETVTESIVHDSIENVVDRVHTDLKGRQDPLSALVRGVDEPWEVCLLKLLVEV
                     VRRSAPGNARDLRADPDGSRHEIEAAFRRAANNRAQLAPLAALLNRLGKFKDYEDRFY
                     ALVRSLPR"
     gene            12378..12869
                     /locus_tag="PKI64_05365"
     CDS             12378..12869
                     /locus_tag="PKI64_05365"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOE00475.1"
                     /translation="MPPTDVPPPLVIRALRKVLPLVTFLFCAALIWALHRWFPDAQWQ
                     FPHARRIARLLAVAGSGLGFGGILAFLRARTSLLPNHPERAATLVTTGIYRVSRNPMY
                     VGLLLVLIAEVVYMNTIPGVMALPLFVAVLQRWQIRPEEEALRAKFGADYAVYARRTR
                     RWL"
     gene            complement(12894..13349)
                     /locus_tag="PKI64_05370"
     CDS             complement(12894..13349)
                     /locus_tag="PKI64_05370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE00476.1"
                     /translation="MSGVKVRYATVAEARRLVDFNRALALESEGLALNETTVSAGVLA
                     VLAHPERGFYLIAEMDGAPVGCLLVTYEWSDWRNRVFWWIASHYVKPEMRCRGVYQAM
                     DEKLQELAAQAGNVCGFRSYVHKDFRSAQDILRSLGMHEKEYLVFEDML"
     gene            complement(13422..14462)
                     /locus_tag="PKI64_05375"
     CDS             complement(13422..14462)
                     /locus_tag="PKI64_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOE00477.1"
                     /translation="MNQTLDIVVRIAKQAWAVLGEMAPWVLGGFAIAGVLSLWLNARV
                     VRKHFGGARFSAILKAVALGVPLPVCSCGVIPLGASLRKHGAGKGPTAAFLMSTPQTG
                     VDSIAVTYGMMGGLFALMRPLAALVSGILCGFAVEVTNGDAPSAGDAPEPELEDEQHP
                     WWQRVLHNGWVVLPRSVGGALVIGAVVSAIVMVLVPPNYVAEKISSPLLQMVLMLAFG
                     IPVYICSTAAVPFALGLLAAGLTPGAALVFLIGGPGVSASTLIAVQRLMGWRAMLVYV
                     LVLALCAIAFGYLMDSLLPAGWLPPGIQAADHCAHGETGMGWTTHVWAALLLGIIGWA
                     KWGPRKRAAHAD"
     gene            14548..15363
                     /gene="lgt"
                     /locus_tag="PKI64_05380"
     CDS             14548..15363
                     /gene="lgt"
                     /locus_tag="PKI64_05380"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOE00478.1"
                     /translation="MHPILFHVFGKPIYTYGVMAAAGFLAGVLTWVWMSRREVRPPGF
                     APDLGFWLMASGIVGSRLAYVAANWSYYRAVPLEIVRIDQGGLIFYGGFLLACVALVL
                     FARHHRLPVWHMADYAIPGLAVGHALGRVGCFFNGCCYGRPAGDSWLGIAYPAVCEPG
                     RLFPGMPLFPVQIMEAGALLVIWLILLHAYARRRRNGAVFALYLLLYPPVRFALEYLR
                     GDERVAWFVLDVAQVTSLLLVLCGILLLVFLPRQVYSPPRKPPSAGPAAAPRP"
     gene            15399..16979
                     /locus_tag="PKI64_05385"
     CDS             15399..16979
                     /locus_tag="PKI64_05385"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOE00479.1"
                     /translation="MSKNLAMETLASLCKRRGFIFQSSELYGGINGFWDYGPLGCELK
                     RNIKDAWWQATVRTREDVVGLEASIIMHPAIWKASGHADGFADPMVDCKACRKRFRAD
                     QLCEEQGLELIKQDKTFALPPGAKCPACGSADLTAPRSFNLMFKTFVGPVEDDSNAAW
                     LRPETAQAIFAQFNNICATARQKVPFGVAQMGKSFRNEINPRNYTFRSREFEQMELEF
                     FIRPDEAVELICGKVTTLADNPDLTGEPQDSWGWEVWHRYWVEQRLAWYRAIGLPAAD
                     LELYWQTGDELAHYARTCVDIEYAFPFGVQELEGIAARGDFDLTQHQLHSGKQMDVFD
                     EALKQAADKLVAAAKEAFTQKVVADWTGRGKTADDARAFVAKLFEGKYVPHVVEPSAG
                     TDRLALALLCNAYDEETLTDDKGKTDTRTVMRFHPTIAPIKLGVFPLVKNRPELYAKA
                     REIFTSLQRRWNCFWDESGAIGRRYRRMDEAGTPWCLTVDFQTLEDGTVTLRDRDSMQ
                     QERLSIPALIAKMEQAIG"
     gene            17010..17864
                     /locus_tag="PKI64_05390"
     CDS             17010..17864
                     /locus_tag="PKI64_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE00480.1"
                     /translation="MFVQLKCTSCGQAFDHDSSSGETFAECPHCNHANALPAAAEPPK
                     PTVLHNAPNLVGTRPCPSCKAPLDREAILCVNCGFNLQTRKKTSGAKPNFGLLLAIGL
                     ILVLMVGGIIYLLQPADTPPPPVFTLEPAKVTTLAAPSQPAASAEAVTAPAEPTPAPA
                     IEAPPAPPPKPTAEELAAQKAVEEEAAFRAKKAQAEQNLRLQLASREPPYQLNDAVEL
                     RRKNGVVVRGTFSGYGGTGPDRVILLATPTGEIGIPLNQLDPPSRRRVDPEYREAFIQ
                     HVLSTRTP"
BASE COUNT         3643 a         5608 c         4911 g         3768 t
ORIGIN      
        1 cgtcctgtgt cccaaggagt tatgactttc catttcagag gtgtcgaact caggagtcat
       61 cgaattgccc gcccaccctt ggtttgtggc tgtcctgttc caccccgaat ggaaatcaca
      121 ggccctgcac ccccaccccc tcttccgcga cctcatccgc gccgctatcc accaccgctc
      181 ccaccacccc ccagcctaac catcccacca ccagacctat tcaacccggg attgccgcgg
      241 ggctgcgccc gctattgcac catgcgttgt tgtccaaaaa gttttttgat tttactgaac
      301 aacgaatttg aagtaacgcc taccgcttgt cgaacatacg caaattgagg cgccctcata
      361 accatccgcg cagtttgtga aatcataggc tcacttttgt aagtgttgag ggcataacga
      421 atagattcct gaattttatc ggcctgctcg agaccggccg cagtaatgtc ggatggatcc
      481 atctcttggg cgtctaccca tatcaataga gtcgaaagag aaatgagaaa atcgaaatat
      541 cgattgtcgc ccccagacct tcttgccaag tctatcactt gttcgattgt gatttgttgc
      601 gtaacgaata tgctcatgtt ggcatcgagt attccggcca ttgccagtaa cgatgtctca
      661 tccgggaatg aagcgctgta attattccgg agctctgaat agtacttggt ggtcatggtg
      721 gcaattaacg tgaaagtact ctcctcgcca acggcatgta acatatccat tgccgtatac
      781 atatcggttt ccgatcctgg gaccacagct acttccaaac gagcgcctgt cctttttaaa
      841 taattatgaa caagatttcg gtaatattgc gaacgaagtt gacctttgcg gttctgatac
      901 tgcgagatgt cgacatcatc ggcctgattc atattcaacc caagcaactc gtcaaattct
      961 ttaacagatg tgtcctcgct tgatgcgcac cgatggatct tcgcctgcct ttcatttaac
     1021 caatgtagta catccgggaa cgcagcaata aaccatagtg gaacagccac tggtcgattg
     1081 attactgatg tatacttttt aacagacggc gaagtgggca gactgcgctg ctcaagatcg
     1141 aatatgctag agaaagacgg aagcccctcc gatagtagtt cgcataaatt tcctccaaaa
     1201 gtttttgggt caatctgtgc atcaacaagt attgatataa catcatcaag ctcttccgag
     1261 aggacaattt caaaggtttg attatcaggt gcgagcaact tgtgccaaat attgtgtggc
     1321 atatttattc tccatggctc acgcaagagc gatgaggcta agtcggcttg ccacctcgga
     1381 cgcccaaaac gtcttgttga acccattaca ttttgtgtga atgggacggc tgagtccaga
     1441 aaattggcgc tttggcgtcg aaaatggcat gttttcaggt ttagggggcc attttggtgc
     1501 gtttttaggc attttggggg tgttttaggc aattttgggg cgaaaaaatg atttttgggg
     1561 gctcatttcg ggaattagga aaaaccagcc agcctcaccg aggccggcta cagcgcttgc
     1621 ggcgtaagat attttttcgt tttttagcgc ccgtttgcgt cttttccgtc gcttgcgttg
     1681 aaattctttg ccttggcgct tggctgggca gccggagcag cccgccgggg atacacccct
     1741 ggcctacaaa gaagcgaccg tttactgtag ctgcggacgg tggccgcgga gctttttccg
     1801 gacaccggcc tcaccgaggc cggctacagt aaggccctgc cgagccttgt ccgctgtgcg
     1861 cagcacaagg ctgctccctg aactctgccg acgagcgtcc tgcgaggcgg cgtcgcttgc
     1921 gttgaaattc ttcttcttgt cggcaggctt gttttcaccc caaaaccgaa acgtggcgcc
     1981 gtgcgccaca atccttcaaa tactgaacta ttttccgtcc ggcgaaatat cgtcgcggct
     2041 taattcgcaa tctttgagaa tctgcgcaag caacccgcgg ccgatagttt cgccacggtg
     2101 aacaggaaca accgtgcagc gtccgtcaag gtgacgtaga taatggtgac tcccctttat
     2161 cctgatgacg ctaaatccaa acttgccgag gatacggata agctcggttc ccttgagcgt
     2221 tggcagcctg ctcatgccat tacagaaacc cgctgaacac cgataaactc attgttaacc
     2281 ggctcatcgc caacctctag gcacagttca atggcttctt taatgcgact caccaattca
     2341 tccagagatt tggcctgggt atggcaccct tttaacgatg gaaccgatgc cacaaaatag
     2401 ccatctgcat cccgctcgat aaccacattg aattcacgca tcttacttgg cctttctcaa
     2461 gtgactggtt tactccaaat caccacttcg cgtcaaacat accattggcc aagcctgttt
     2521 gcacagctcg ttgacccaac atcataattg gcgccttggc gtcgaaaatg ggttcttcgt
     2581 aaaattttat ggaatgaatt ggctctgacc tcaaaaattt ggtggctcag ccgtcttgct
     2641 acatattaaa gaacggcagg atttcttaat taatctctcg atagcgttgg gatttggtgt
     2701 gctgcgccct ccgctccgcg agaatgggcc tcccatagct cgtcggagcg gagtggaggg
     2761 cggggcggtg cgatgccgta attaccataa aattctacga agaacccgaa aatggctcat
     2821 ttttcggttt ttggggtgtt ttggggcatt tttgggacga tttgagcgat tttctgacgc
     2881 aaaaatggtt ttggagcgtt cggagcgatt ttcgggcgaa aaaatgattt ttgggggctc
     2941 atttcgggaa ttaggaaaaa cggttcctcg ctaattccag tggccagagt ccggaaaatg
     3001 gttcagtttc aggggcatga caacaccgga aaaattgtgt agtgaactgt tgggattagg
     3061 cgatgtatgg tggccatgct ctacttcgtt gctggaaaac taccctcacc cgccatgctc
     3121 agccactgaa aacagcgagg aaccggaaaa accagccggc ctcagcgaga ccggccacag
     3181 cgcctgcgag gcgagatatt tttcgttttt tgcgcccgtt tgcgtctttg gcgccgtttg
     3241 cgttgaaatt cttgatcctt ggcggcttgg cgagatttct tgccctctat agccgcggac
     3301 ggtggacgcg gggctttttc cggacaccgg cctcaccgag gccggccaca gcgcctgcgg
     3361 cgcgagatat tttttcattt ttttgcgccc gtttgcgtct ttggtgccgc ttgcgttgaa
     3421 attcttcttg gtgcttgaaa tcctcgacaa cctccaaacc gccctcgacc actttcaaac
     3481 catctccgaa gccctcacca aaacctgaat attgaagtca tttttcttgt tcgggattgg
     3541 attgtagggc cggaacggtc gaagaccttc cagccttttc cggcgggaag gcctctttcc
     3601 ccaaccattc ccgcccttca aaaaagcgaa tcagcaaaat tgttgggcgt cccaatacgg
     3661 aacgcgagaa acagccgggc gcccggtgtg aaatcttagc ggcgtggctt gtcttcgccc
     3721 cctcgctcct catcctccaa accccttgga tgctcgccaa acatggcccg ccctatcatg
     3781 ctccagttca aaacggcggc ttgcacatcc gactcccccg cttggggaag cgccggctcc
     3841 cgccgaagca aaagcacatc caccaaggcg gaaaccaaca ccgcatctgt tttatcgctt
     3901 gtttcgttgt actccgccgg atcgcgattt acatccgccc taaccatcct ccacccacga
     3961 ttttccagaa caaagcgcac cacataaatg cagaagcccg tccaactgcg gtgaaagtat
     4021 agcgtgccct cttcccaata aataaaccat ttgtcttcca tttgctccgg caccaccccc
     4081 cgttgaatca gctccatctc ttcttctgaa aacaagcgat ccaacgcaat cgtcgtcctc
     4141 ttcgccggaa gctcctccct cgaccaatcc cttgccctcg ccgctctcat gttgcacctc
     4201 tccatccatc cataaaaaac aacacccgta agctcttgct caatctctcc attcttctta
     4261 cccaaggcat ttgagtccag aaaattgacg ttttagcgtc aaaaacggct aatttcccgg
     4321 gttttgaggc cttttttagg tgttttgggg cattttgggg gtatttttgg caattttcgg
     4381 gcaaaaaaat ggtttcgggt gtcgcatttc gggagccagg aaaaatcagc cagcctcagc
     4441 gtgaccggct acagcgcctg cggcgcgaga tattttttct tttttttgcg cccgtttgcg
     4501 ccttttccgt cgcttgcgtt gaaattcttt gccttggcgc ttggctgggc agccggagca
     4561 gcccgccggg gatacacccc tggcctacaa agaagcgacc gtttactgta gctgcggacg
     4621 gtggccgcgg agctttttcc agacaccggc ctcaccgagg ccggctacag taaggccatg
     4681 cctagccttg cccgctgtgc gcagcacaat gctactccct gaactctgaa ccctgccgac
     4741 gagcatcccg cgaggcggca gccgcgacaa attttcaggc attacggcca tgtcgtgcta
     4801 gctcagccat gatttcttca agaccatgca agcgatagcg ttggcaggtg gtcataatat
     4861 catgaaggtc agcacccggg ttgtgccgca acaactccaa aatatctgcc tttgatttaa
     4921 gtccgccggc atacagcttg agggcaacca attgtggcag cggcgccagc ttaagagcgc
     4981 tgcccggacg caaaagcatc gtggaatctc gaatggcgtc ttgaatcacc gcggggaagc
     5041 gatcgcaaaa attaacgatt tgcaccaccc caaaaccgcc cgaaacattg accacgccgc
     5101 ccaaaggggc gtccgcgtca ggcgcgctaa gttcggcagt caaacccgca gcgttaagcg
     5161 actccgtcac cttctgcaaa acaggaagcg ccgcgctcac gccaatatca ataccctgag
     5221 tatgccgcac ataatggtgc gccgccagtg ccactgcgcc aatcacaacc gaatcaacat
     5281 tgtgttgctt caggatggca accacctgtt ccgcagcttg caatatcggc aacaattttc
     5341 tcataaaatc agggctccgt atcgccgata acaggattaa gccaggcaaa ctgttctttc
     5401 atcgttagcg cttcctcgat gcgtgcctca atggacaagc tttgcaaccg cgccaactcc
     5461 cgggcaaacg attggtccgc actaacactg ggaatcatcg tcgggaaacc aggctgtgtt
     5521 gaagctgcgc caacttcctg atctttgact actttgaatc cctgcgtaat ttttcgccta
     5581 ctcgtcatgg cctctttttt attgccgatc attgcggcat gtcatattcg cgcttcaaac
     5641 gccttgctca atatcgtcat tctgcttggt cgcgacgttt gcgtccagcc agaatcagtc
     5701 atcagcacag ggggaagatg gttgcaggtt acaggttgca ggttacaggt taaaggttgc
     5761 aggtttcagg aagacagccg gaacagcccg ccggggatta caaccccggc ctacaaagac
     5821 gtggctgttt actgtagccg cggacggtgg ccgcggggct ttttccggac accgcctcaa
     5881 cgaggccggc tacaaaaagg ccatgccgag ccttgtccgc tgtgcgcagc acctgcatgc
     5941 ggaggatttc ttgcgaattt tggcggggct gttgcgccgt cgcaaggcga cggtggggcc
     6001 agggttcaga agtcagagga agccaccggg ccgacagccc cgcgttcagc gagcgcggca
     6061 acagtaaggc cctgccgagc cttgcccgct gtgcgcagca caaggctgct ccctgaactc
     6121 tgaaccctga accctttaaa aaagtgtccg cgaaggcgtt ggggttctgg ggggcgcggg
     6181 ctagtacagg tctttattga cgttttgggt gatggtggtg gtggtgcgac gccaaccgcg
     6241 caccattccg gccagccatt gccaaatgaa ccatcccaac agcgcgaccg aaatccatcc
     6301 tgcgattgtc aacgcgagtt ccatcagatt atctccggtt gttcagcttg cacgatgtcc
     6361 ggcagcgtag ccgcttcctg gtgagccgtc aatgcgcagt ctgccgcaac caactagacg
     6421 ccgtaggtgg tggacgcggt ggtgcctccc cgccccgtcc agttggtgtg gaagaattgg
     6481 ccccgcggct tgtccacgcg ttcataggtg tgggcgccga agtaatcgcg ctgggcctgc
     6541 aagagattgg ccggcaaccg ggcgctgcgg taggcgtcgt agtagctcaa agcggtgccg
     6601 atggcgggca gggggatgcc tgcttccgcg gcagcccgga ccacccgccg ccatgccgct
     6661 tgcgtttggc cgacgacatc acggaaatag ggcgccaaaa gcagattggc cagcgcgggt
     6721 tcggcatcga aggcttcctt gattttgccc aaaaagcgcg cgcggataat gcagccgccg
     6781 cgccacagga gggcgatatc gccgaagttc aggttccacc cgtgctcggc ggaggccgcg
     6841 cgcaagagtt gatagccttg ggcataagaa caaattttgg cggcgtggac ggcctgttcc
     6901 aagtcggcca caaaagcagc acggtccacg gtgatttttc cattcggccc cggcaaaata
     6961 gcagcggcgg caacccgttc ttccttgatg gccgacaagc aacgcgcaaa cacggcttcg
     7021 gcaatttgcg gaatacccac gccaaggtcc agccccgctt gcgaggtcca tttgcccgtg
     7081 cccttttggc cggcggcatc caaaatcata tccaccaagg ggcggccggt ttcatcatcc
     7141 acctgggtca ggatgtcggc ggtgatttca atcaggtagg aatccaacgc gccggcattc
     7201 cattgcttga acaccgcgcc catttcggca ggggtcatgc ccaagccgac ggacagcaaa
     7261 tggtaggctt cggcgatgag ctgcatgtcg ccatattcaa tgccgttgtg caccattttg
     7321 acgaagtgac cggaaccatc gggccccagc cattcgcagc agggggtgcc atcgtccacc
     7381 ttggcggcaa tggcttgaaa aatgggtttg aggtgaggcc aggccgccgc gtggccgccg
     7441 ggcataatgg acgggccggt cagcgcaccc tcttcaccgc cagacacacc cgcgccaacg
     7501 tagagcaggc tgttcgcttc cgccgccgcg caacgccgga tggtatcggg gtaatggctg
     7561 ttaccgccat caatgaggat atcgcccggc tccagcagcg ggatcagttc ggcgatcagg
     7621 tcatccaccg gttttccagc gcgcaccatc atcatgattt tgcgcggtcg ggccagcgcc
     7681 cggaccaact ccgccggtct gtggcagccg atcaggtttt tgtctttgcc gcgaccgcca
     7741 aggaaggcat ccaccttggc tactgtccgg ttgtagcagg ccacggtgaa gcccttgttc
     7801 tccatgttga gaatcaggtt ctcgcccatt acggcgagac ccaccacgcc aatatccgcc
     7861 aatgccttca tgctccgcgt cctttcaaat ccagcagccg tcttcgccgc agcccaagcg
     7921 ccattcgtgg tcatcgcgcg ccagcagttc atcggcggcg gcggggcccc acgatttgcg
     7981 cgggtagata tgcaacggcg agcgttcagg gtgctcctcc caaccggcca aaatggggtc
     8041 aatcaaccgc caggcaattt cgatgccgtc ggcccgggcg aacagcgagg cgtcgccctt
     8101 gatggcatcc agcaggaggc gttcataggc atcgggcagg acgatgtccg ggaacgcctt
     8161 gcggtagctg aaatccatgt tggccgagcg tgattcggcg atggtatcgg gcaccttggt
     8221 ttcgaagcgc aggtgcaccc cttcttccgg ctgaatacaa atggagagca tgttgggggt
     8281 gaaactttgg ttgcgcgcca agccgaacat gacatccggc ggcgcctgga attccacgat
     8341 gatggagctg ccgcgcgcgg ccatggcctt gccggaacgc agataaaacg gcacgccctg
     8401 ccaacgccag ttgtcaatgt agagtttcaa cgcggcatag gtcggggttt gcgagtcggg
     8461 tgccactcgc gcggcctcgc gatagccctc atattggccg cgcacggcat cttcgagccg
     8521 gatggcgcgg atattgccca gtaccttggc tttctcgttg cgcaccgcgt tggcatccag
     8581 cgaagcgggc ggttccatgg tcaccagcga aagcaattgc agcaggtggt tctggaacat
     8641 gtcgcgcacc acgccggcct gatcgtaata gccaccgcga gtgcctacat ccacatcctc
     8701 ggccacggtg atttggatgt tggaaatgta gcgccggttc cacaccggct cgaacagcgt
     8761 gttggcaaaa cgcaggaaca ggatgttttg cgcggtttct ttgccgagat agtgatcaat
     8821 gcggtacacc tgatgttccc ggaacgactg gtgcacccgg tggttcaacg cgacggcgga
     8881 agcgagatca tgcccgaagg gtttttcgat gatgatccgc cgccagcctt cggtttcggc
     8941 cgtcatgttg gcggcggcca gatagtcgca ggcggggcca tagaactccg gcgcggtggc
     9001 cagataatag aggcggttgg cgggcttgcc ttcaatctcg cgcaaccgcc gatccacctc
     9061 gtggtaatct tccggcactt ccaggtcgcc ccgcacgtag cggatgcgcg gcgcaaactc
     9121 ggcccacgcc ttttcatcga aggggccgcc atagcggcgc acgccctcgc gcaggtgttc
     9181 gcggaaaaaa tcatcgctcc agtcgcgacg cgccacgcca acgatccgtg tgcggtccca
     9241 caagcgccct tttttacggt tgtggaacag cgcggggatc aacttgcgct gggtcaagtc
     9301 gccggtggcg ccaaaaatga cgatggtggt ggcttccgcc tggggaatct cattactcat
     9361 gatgggactc gctggttggt tgttccgctg cttgctcatc aataaaccat tcacaggggc
     9421 cagcggtcaa atgcacacgt tgcgccggca acaaatcccc gtttgctacg gcgcggacct
     9481 tctgcacgac gcgggccttg tcccggccca tggccacaaa caaggcctgc cgcgcgtggt
     9541 tcaggaccgg cagcgtcaac gtcacgcggg ttggtggcgg cttgggcgac tgccacaccg
     9601 gcgcggccca gtgcaccatt tccgtcaaga ccggattacc cgggaataac gatgccgtat
     9661 gtccatcccc ccccaccccc agcaaaatga catcaaaccg cggccatgcg tcgccatccg
     9721 cggcaaattc gcggcgcagc gccgcctcgt aagcacgcgc ggcttcctcc ggcagcagcg
     9781 tgccatccac aggaaaaatc tgatctgcgg ggatggggac atcatccaaa aactcagcgc
     9841 gggcagcgcc gtagttgctg ttggggtcat ccgcggccac gcagcgttca tctgcccaat
     9901 acacccgcca ggccccccag tatgcattca actccggcag caagcggaat cccgccgcca
     9961 gccgcagcat ggcggagccg ccagacaacg cgatgcggaa ccgcccgcgc tcctgcaccg
    10021 cagttgccgc ggccgatgcc acccgccgcg ccagcgccgg ggacagctcg gttggcccgc
    10081 gaaacacatg aaactggtgt gccgggttgt tcatcgtcta catcctctca tcgcctcaag
    10141 agccgcatga ttcgccacaa ccctcaccat tcccgtgcca ccagcgcctg ccaatcctgt
    10201 tggtaattgt tgtgccaaac cttgttgacc ggatcaaaga agcaaaaggc cacattccac
    10261 ttggccgagg atggagtggg gaatgtggcc ttccacaatc cggtggtggc atccttttcc
    10321 atcggcacat ccatcagaaa atccgcaaaa ttatcacgtc cgacatgaac actgatttgc
    10381 tgcgcatcgg ccaacgggcc gccggcggcg ttgtaccaca cggtgcaatc ctcaccccgc
    10441 accgggaaaa tcggttccac gcgcacatgg cggtgcgcct tgtattccgc gcggtcgaac
    10501 accgttccgt ccgtcgcggt tgccgccaac tgaatccggt cgccggccac gttgaattgc
    10561 acaaagtgat acaggctggt ggcgaatagg agattctcgt gcttttccgg cacccagtac
    10621 aggcgaccgc caccaccgcc actcaccaga taattcacgc cggccagcgg cttggtccgc
    10681 tgataggtgt gggcgtggcc gttcagcacc aagtctgcct cgtaacgggt aaataacggc
    10741 acccaccact cccgcacctg ctcctgcccc tcccgctcgc catacgaaaa cggcgggcgg
    10801 tggaatgccg gaatgaccca ggtaatctcc ggatcatacg ccgcgccttg cagctctcgc
    10861 gccagccagg cgatgcggct ggggatttgc tcttccaccg aatccagcat gacaaaccgg
    10921 atcggcccca catccagcac gtaatcatgg tggctcggca gctcgaacaa ccggtgccag
    10981 aacgagtccg gattttccgg ctcatcatgg ttgccggtca ccacctgaaa tacaaagtgg
    11041 gctagttccg gctccgtgca gtcaaaaaac aactgccagc tctgcacgcc gttggcatag
    11101 tccgaggaat aacgcgaatc gcccaaatcg ccggtggaca gcacgaaatc cggattggcc
    11161 cgcaccacgg cttgcgccac ctgtttggcc gcctccacat tgatcccccc ttgtaaatcg
    11221 ttgtgcagcg caaaggtgaa tggcgtggtc ttgtcggccg gcgccgtcca aaaacgcccc
    11281 tcagcttgat agccgtccca cgaagccagc cggtagcggt aatgcgtccc cggttccaaa
    11341 tcccgcaggg tgaatacatg acgccgcacc ggctgttctt gggccacatg ctgccagata
    11401 gcgccgtcag ttgaatattc cacccaggcg ggttctgccg ccgatctctc ccaagccaag
    11461 gtgacactag tccgcgggtc ttccgtccat gtggccatca ccggtaaatc ccatgcgcgt
    11521 tctgcggctc ttgtcacaac cgctgcccac gccgcaaaca cccacaacca acgtgcaatt
    11581 ttcatggtca tcacgctacc gcacccctgc ccgtttggca aaaccaactt ccggcttcca
    11641 gccttgcgcc gccgcccgca atgccgtagc gtgtgaccac gatgtctgca tctcctcaag
    11701 aatttgattt ttggtacgcg gtcaacaaca ccgaagtcgt catgcccccg cggggccgtt
    11761 tggaaacctt tggctccacg ctcatccatt attggctggt gaccgagctg atggacgcca
    11821 tcgggcaggt acgcatccgc gaggggcgca tccaatcgtt ccggccagaa atcctcacgc
    11881 cgcagagttt cagcgaatcg ccgctggagg gttttcagga ggaacaggcg gaagatttca
    11941 tccgctggct gcgcgagcac gaatccgact tcatgttgtt gaaatacggg ttccgcatcc
    12001 ggcatgagac ggtcaccgag tccatcgtgc atgattcaat tgaaaacgtg gtggaccggg
    12061 tccacaccga tttgaagggt cggcaggatc cgctttccgc cctggtgcgc ggcgtggatg
    12121 aaccgtggga ggtctgcctg ctcaagctgc tggtggaggt ggtgcggcgt tctgcgccgg
    12181 gcaacgcgcg tgacctgcgc gcggacccgg acggctcgcg ccatgagatt gaggccgcct
    12241 tccgccgcgc ggccaacaac cgcgcccaac tggcgccgct ggccgcgctg ctgaaccgct
    12301 tgggcaaatt caaggactac gaggatcgtt tctatgccct cgtgcgttca ttgccccgct
    12361 aacctgcgcc ccaccccatg ccgccgactg acgtgccacc gcctcttgtg atccgggcgc
    12421 tgcgcaaagt gctgcccttg gtaaccttcc tgttttgtgc ggcgttgatt tgggcgttgc
    12481 atcgctggtt tcccgatgcg caatggcagt ttccccatgc aagacgcatc gcccggttgt
    12541 tggccgtcgc cggcagcggg ctgggcttcg gcggcatctt ggcgtttcta cgcgcccgca
    12601 cgagtctgtt gccgaatcac ccggagcgcg ccgcgacatt ggtcaccacg ggcatctacc
    12661 gcgtgagccg caatccgatg tatgtcggcc tgctgctggt gctgattgcg gaagtggttt
    12721 atatgaatac cattcccggt gtgatggccc tgccgctttt tgtggcggta ctccagcgct
    12781 ggcaaatccg cccggaagag gaagctctgc gcgccaagtt tggcgcggac tacgccgttt
    12841 atgcacggcg cacacgccgc tggctctgag cccccccttc ccgcactgtg gggttagagc
    12901 atatcctcga aaacgaggta ttccttctcg tgcatcccca gactgcgcag gatatcttgc
    12961 gccgagcgga aatccttgtg gacgtaggaa cggaagccgc acacattacc ggcctgcgcc
    13021 gccagttcct gtaacttttc atccatggcc tgatacaccc cgcggcagcg catctccggc
    13081 ttcacgtaat ggctggcaat ccaccagaaa acgcggttgc gccagtcgct ccattcatag
    13141 gttaccagca ggcagcccac cggcgcgcca tccatttccg caatcaaata gaacccccgc
    13201 tccgggtggg ccagcacggc caagaccccc gctgataccg tggtttcgtt gagggccaag
    13261 ccctcgcttt ccaacgccag cgcccggttg aaatccacca gccgccgcgc ctccgccacc
    13321 gttgcatacc gcactttgac tccactcatg atgcgcctcc ctttgctcga gttgcatcat
    13381 accccccacc cctcgcccgc gacaatagaa aaaaggccgg ttcagtccgc gtgcgccgcg
    13441 cgtttacgcg gcccccattt ggcccagcca ataatgccca gcagcagcgc ggcccaaacg
    13501 tgcgtggtcc agcccatgcc ggtctcgcca tgggcgcagt ggtccgccgc ctgtattccg
    13561 ggcggcagcc agcccgccgg caggaggcta tccatcaggt agccgaaagc aattgcacag
    13621 agcgccaaca ccagcacgta gaccagcatg gcccgccagc ccatcagccg ctgaacggca
    13681 atcagcgtcg aggcgctcac gcccggcccg ccgatcagga acaccagcgc ggcgccggga
    13741 gtcaatcccg cggccagcaa gcccagtgcg aagggtaccg ccgccgtgga gcagatatat
    13801 accggaatgc cgaacgccag catcagcacc atctgtaaca gcgggctgga gattttttcc
    13861 gccacgtaat ttggcggcac cagcaccatc acgatggccg acaccactgc gccaatcacc
    13921 agcgccccgc caacggaccg cggcaacacc acccagccgt tatgcagcac gcgctgccac
    13981 cacgggtgct gctcatcttc cagttcgggt tccggcgcgt caccggcgga tggcgcatcg
    14041 ccattggtca cttccacggc gaaaccgcac aggatgcccg acaccagcgc ggccagcggg
    14101 cgcatcaacg cgaacaggcc gcccatcatg ccgtaggtca cggcgatgct atccaccccg
    14161 gtctgggggg tactcatcaa gaaagcggcg gtcggcccct ttcccgctcc gtgtttacgc
    14221 agcgaagcgc cgagtgggat cacgccgcat gaacacaccg gaagcggcac ccccaacgcc
    14281 accgccttca aaatggcgct gaaccgcgcg ccgccaaagt gcttgcgcac cacccgggcg
    14341 ttcagccaaa gcgagagcac accggcgatg gcaaaacccc cgagcaccca aggggccatt
    14401 tcgcccagta ccgcccaagc ctgcttggcg atgcgtacca caatgtccag cgtttgattc
    14461 atgacaggcg aatctactgc acgttttggc caaccgccac tcaaggcgct ggatttgacg
    14521 ccaagaacct gttaaattgc caaagaaatg cacccgattt tgttccatgt gttcggcaag
    14581 cccatctaca cctatggcgt gatggcggcg gccggttttt tggccggggt gctcacgtgg
    14641 gtgtggatga gtcgccgcga ggtgcgcccg ccgggcttcg cccccgatct tgggttttgg
    14701 ctgatggcca gcggcattgt cggttcgcgt ttggcttacg tggcggccaa ctggtcgtat
    14761 taccgcgccg tgccattgga gatcgtccgc attgaccaag gcggcctgat tttctatggc
    14821 gggtttttgt tggcgtgcgt ggcgctggtg ctgtttgctc ggcatcaccg gttgccggtg
    14881 tggcatatgg cggattacgc catcccgggg ctggccgtcg gccatgcgct gggccgcgtg
    14941 ggctgcttct tcaacggctg ctgctatggc cgcccggccg gcgattcctg gctgggcatt
    15001 gcctatccgg cggtttgcga gccgggccgc ttgtttcccg gtatgccgct gttccccgtg
    15061 caaatcatgg aggccggcgc gctgctggtg atttggttga tcctgctgca cgcctacgca
    15121 cgacgccggc gcaacggcgc ggttttcgcg ctctacctgt tgctctaccc gcccgtacgc
    15181 tttgccttgg aatatctgcg cggcgatgag cgcgtggcgt ggttcgtgct ggatgtggcg
    15241 caagtcactt cgctgctgct cgtgttgtgc ggcatcctac tgctggtctt cctgccccgg
    15301 caggtatatt ccccgccgcg caaaccgcca agcgctggtc ccgccgcggc cccccgccct
    15361 tgacgcgccc ggcggtttag cgcacgatgc gcgccattat gagcaaaaac ctggccatgg
    15421 aaaccttggc ttcgctttgc aagcggcgcg ggttcatttt tcaatcttct gaattatacg
    15481 gcggcatcaa cggcttctgg gactacggcc cgctcggttg cgagctgaag cgcaacatca
    15541 aggatgcctg gtggcaggcc accgtccgca cccgcgaaga tgtcgtcggc ttggaggcct
    15601 ccatcatcat gcacccggcc atctggaagg cttccggcca cgccgacggt tttgccgacc
    15661 ccatggtgga ctgcaaggcc tgccggaagc gtttccgcgc cgaccaactg tgcgaggagc
    15721 aaggcctcga actcatcaag caggataaaa cctttgccct cccccccggc gccaaatgcc
    15781 ccgcgtgcgg atccgccgat ctcaccgccc cgcgcagttt caacctgatg ttcaagacct
    15841 tcgttggccc ggtggaggat gactccaacg ccgcttggct gcgcccggaa actgcgcagg
    15901 ccatctttgc gcaattcaac aacatttgcg ccaccgcccg ccaaaaagta ccgttcggcg
    15961 tggcccagat gggcaagagc ttccgcaacg aaatcaatcc gcgcaactac accttccgct
    16021 cgcgcgagtt cgagcaaatg gaattggaat tttttatccg ccccgatgag gctgttgagc
    16081 tgatttgcgg caaggtcacg actcttgccg ataaccccga tctgaccggc gaaccgcagg
    16141 attcgtgggg ctgggaagtg tggcaccgct actgggtcga gcagcgcctt gcgtggtatc
    16201 gcgccatcgg cctgcccgcc gccgatctcg aactctattg gcagaccggc gatgaactcg
    16261 cccactacgc ccgcacctgc gtggacatcg aatacgcctt ccccttcggc gtgcaagagc
    16321 tcgagggcat tgccgcccgc ggcgattttg atcttaccca acatcaattg cactccggca
    16381 agcaaatgga tgtgtttgat gaagccctca agcaggccgc cgacaagctg gttgccgccg
    16441 ccaaggaggc tttcacccaa aaggtggtgg ccgattggac cgggcgcggc aaaaccgccg
    16501 atgacgcccg cgcatttgtc gccaagctgt ttgaaggcaa atatgtcccg catgtcgtgg
    16561 agccttccgc cggcaccgac cgccttgcct tggcgctgct gtgcaatgcc tacgacgaag
    16621 aaaccctcac cgacgacaaa ggcaaaaccg acacccgcac cgtcatgcga ttccatccga
    16681 ccatcgcccc catcaagttg ggcgtgttcc ccctcgtgaa gaacaggccg gagctatatg
    16741 ccaaggcgcg cgaaattttc acttcgctcc aacggcgctg gaactgcttc tgggatgaat
    16801 ccggcgccat tggccggcgc tatcgtcgca tggatgaagc cggtacgccg tggtgcctca
    16861 ccgtggattt ccaaactctg gaagatggca ccgtcaccct gcgcgaccgc gactccatgc
    16921 agcaggaacg gctttcgatt cccgcgttga ttgcaaaaat ggaacaggcc atcggctagc
    16981 tcacgacacc ccaacccaag gaggccccca tgtttgttca actcaaatgc acttcatgcg
    17041 gccaggcctt cgaccatgac agctcatccg gcgagacctt cgccgaatgt ccgcactgca
    17101 accacgccaa cgcgctgccc gccgccgccg agccacccaa gccgacggtt ttgcacaacg
    17161 cgcccaactt ggtgggaacg cgcccttgtc catcctgcaa agcccctttg gaccgggagg
    17221 ctattctctg cgtgaattgc gggttcaatc tccagacccg caaaaagaca tctggcgcca
    17281 agcctaactt tggcctgctg ctggccatcg gactaatttt ggtgttgatg gtgggcggca
    17341 tcatttatct gctgcagccc gctgacacgc ccccgccgcc ggtcttcacg ctcgaaccag
    17401 ccaaggtcac caccctcgca gcccccagcc aaccggcggc atcggctgag gccgtcacag
    17461 ctcccgccga gcccacaccg gcgccagcga ttgaagcgcc ccccgccccg ccgcccaaac
    17521 ccacggccga ggaactcgcc gcgcagaaag ctgtcgaaga agaggccgct tttcgcgcga
    17581 aaaaggcgca agcggaacaa aatttgcggt tgcagttggc cagccgtgaa ccgccctatc
    17641 aactcaacga cgccgtcgaa ctccgccgga aaaatggcgt ggtggttaga ggcaccttca
    17701 gcggctacgg cggcaccgga ccggatcgcg tcattctact ggccaccccc accggcgaaa
    17761 tcggcattcc gctgaatcag cttgatcccc cctcccggcg gcgcgtggac cctgaatatc
    17821 gcgaagcctt catccaacac gtactctcca cccgcacacc atagcacggg attgaattgt
    17881 agatgctatg gggtttcgtt catgcatcat ggtgatgtgg atcaaccaca
//
LOCUS       DAOPNP010000042        17699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3817_length_17699_cov_6.940320, whole genome shotgun sequence.
ACCESSION   DAOPNP010000042 DAOPNP010000000
VERSION     DAOPNP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17699
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3817_length_17699_cov_6.940320"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1745)
                     /locus_tag="PKI64_05395"
     CDS             complement(132..1745)
                     /locus_tag="PKI64_05395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE00481.1"
                     /translation="MNPPCAWVHLRIPPSLERMRIVQDAACNLAAECGFPAERQRPME
                     LAIEEVITTILRYITDEADVADAVLDIRMKVDAPVLRVSIVNEGLPFDLSEVPEYDAG
                     QLSRLAADSDSAAASGGIRQDHFSSGLALFLLKKATDRCQIQYRGKEGNEFCLEWFLP
                     GAHIAEQAGEEATELAGAAQTEAAAAPPEPIGQLRVLTEADAIGFCRLMYRSYGSTYP
                     NADFYFPERVRAHQQSGRIKSWGAVTPSGRVVGHLALMKDSPAAPAVEWGAVVVDPLW
                     RSEGLMKKLLAAAIESMGARTESVLYAHAVTSHPYTQKTCNRFGFAPTALLLGFIVDN
                     IRFRGIQENAAYRETIFVASRIFRALPRQRLYPPPWHRDTIARLLAGSDLSLAELAAT
                     ADDEAAAAVPAQSTFATTTIASINIAILDVPAVGADFAAVLGFELRRLCRARVDVIYL
                     TLDLADPNVVGAVKAAEELGFFLGGWLPMQPFAYGLTFQYLNTTKVDFKNIATDGEQA
                     TWLKGVIQREQQRIEHGLYGGHAPGACPD"
     gene            complement(1742..3022)
                     /locus_tag="PKI64_05400"
     CDS             complement(1742..3022)
                     /locus_tag="PKI64_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOE00482.1"
                     /translation="MNSATRKLEEHLQFVRAHSPYFAEAWRGAWPEGAPCPGLAALPV
                     TDHAGYWAANSIVDNRLLTAPHDSGIVFKSGGTTGNPKFSFFSNEDWREFCGVFGGGL
                     RRLGLRPGERVANLFYGGQLYASLLFFGRSLEESGVGVHYPLAGFVPPEELIHTMIQF
                     RIHTLAGVPTTILHFLPELIRAGRENIELRRVLFAGESMYPDQIAAVQAAYPGCRVQS
                     VGIAGVDFGEIGWCDESCGPGEHYVFDESIVLEILDEENRPIEEPGVPGAIHATNFQR
                     KLMPIIRYPVGDMGMWLEPVTGKPRRFQLLGRTEKGARVGPMTLYLEDFQAILSRLAA
                     QTGHTGFQLVVDHIEQRDRCTLRIAVPRPDAMPAALADAIRDALYTERPMFPDSIGNQ
                     IIHPLVIEWVEPHTLLRNPRTGKLRRLVDRRFEA"
     gene            complement(3027..5585)
                     /locus_tag="PKI64_05405"
     CDS             complement(3027..5585)
                     /locus_tag="PKI64_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOE00483.1"
                     /translation="MKASTGAAAELRRHFWFGEWLDDAGLDAWLEERGAGESRLAAHL
                     SRVLLMPFPMADFLEASDAIAASLVPGNPFYERCREIVLRVAPAEEAESLFAAIRGTL
                     ARGAVQEKMRSELGMNPPGVIARRYPGRQFEAWFPLGCVVHVMPSNVFSAPVLGLMEG
                     LMAGNVNIVKVSARDTEFAAVFAEELCRLDPGGRLRDYVAVARLSSGDTARMGRLFGI
                     ADGVSAWGGERAIAAIREAIPSGTRLISWGHKVSFTYLAAECLTDDAQLEVTLRAVAM
                     DVCRLDQQACSSPQTLFVEVDRDEDAARKIAARLAAHLAEVSPTIPGQPPSASEQAEI
                     TSVTAVARAEKALGLTELFEDAQGRWRVLFDARAGLRPSPLYRTIWVKPIRRAELTAT
                     LRPMRAWLQTCGLSCGLESLADLSRALFAAGVTRVCHAGEMVDSYIGAPHDGVYALQQ
                     LTRRVSLDGPAEAEGVGCLNELETRAAASAPTGPIQDKNAFMKLAETVERPDLVVKSG
                     GSSGKTVYSLYSWADYHQQMMWTAHGLVAAGLETARDRVMNCYTAGYLYGSLISFWTI
                     LEFLRVPQYPMGMIDDYEYIADVIVSNRVNTLLGVPSHLLGLFNACTGRLRGKIEKVF
                     FGGEKMTVAQRAFLMKDCGVSIVRSAVYGSNDAGPMGYQCPHCVGGQHHLLSALVKME
                     IVALDEDRPVAPGETGRLLLTPITREYPRVNRYEIGDTGRWVPEPCPCGRTDPRFELL
                     GRTGDIFKAAGPFFNYRRFVDILETKLGHTGPVQIHLAEEGHTTVLEVWLTAPPDAPT
                     PAEIEELLRLNYAEIQYAYDMGMAFRHRVRVVEDAEIKRNPASGKVIPICDQRR"
     gene            complement(5640..6446)
                     /locus_tag="PKI64_05410"
     CDS             complement(5640..6446)
                     /locus_tag="PKI64_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00484.1"
                     /translation="MTDASAPTWEDQVIEAMREFATAYGTVYTKKERQIAAHFEIGCL
                     LALIEFYEKAGFVGEVVNMATDGSYRYLTTPNGNPDKFSYMRLRRGEEECIEIRQQVR
                     ISSHVGTDISFTPDIVVTPQAATIGKRKDVDYASGKRTFYFVDSKDVIAAHECKSMVP
                     FPELLVSFIGMLMAGHEWIERIDPKDVMTEEGHHLAPCLFVGGSARSLHLRMIKGLKD
                     AYPMNIVVGMHSGTWNLLGDNADIRRMKNPLMVEQGGPGYPPQGAGYSDR"
     gene            complement(6890..8068)
                     /locus_tag="PKI64_05415"
     CDS             complement(6890..8068)
                     /locus_tag="PKI64_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthase"
                     /protein_id="HOE00485.1"
                     /translation="MLDQPSAPVSNDIPDSVRRFCAIAVPYAADEKYDGLFVEAMRDI
                     TALHMERSPWYRRFAEQAGMKPERIRTMADVLALPPVHANFFKYHEIRSIHESQVKTH
                     LTSSGTTGQKSQVFFDGFTLEWARVMAKHEMVWRGVLDDRPANYLLNFYEPYAGLKVG
                     TSNTMAYLMSYAPAAEQFWTLRYQGDGRHEFDPFGADACLERWAQGSAPVRILGFPYY
                     LYFLLERRRARGQADLKLPPGSLLILGGGWKGYADKSIAKDDFFAQIERQLGIDPERI
                     IEIFGSVEHSVPYTNCPRHHLHQPTWSRVVIRDLKTLAPVPDGTPGFLSFLSPYIPAT
                     PAHSVVMGDLAVRHPAGSCPLDTYPTPWFEILGRAGTSANRSCAAAAAELLKTHCSTW
                     "
     gene            8239..8919
                     /locus_tag="PKI64_05420"
     CDS             8239..8919
                     /locus_tag="PKI64_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOE00486.1"
                     /translation="MAKSGQNEQGEPLIPKHGGFRHLKGFQVAQLVYDVTVRFCDKYI
                     DRRSRTHDQMVQAARSGVQNIAEGSVASGTSKKMELKLTNVARASLAELRLDYEDFLR
                     QRDLKLWPQTDSRRQALVARRCATADEVAAWVVEVAQNGENGQDGRNGRDGHGGPSMQ
                     SMESMRAVLFPEISANAAVVLIGVAMALLDRQIQALAAAFATEGGFTERLYRTRQSSR
                     AERGKEPN"
     gene            complement(8916..9719)
                     /gene="trpA"
                     /locus_tag="PKI64_05425"
     CDS             complement(8916..9719)
                     /gene="trpA"
                     /locus_tag="PKI64_05425"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOE00487.1"
                     /translation="MNRITQTFQRLATQNRKALIGYITAGDPDYATSLGILRAAATSG
                     LDILELGVPFSDPTADGPAIQAASQRALRTGMTLSRTLQLAADLRRHTDIPLMLFSYY
                     NPIYKYGLERLATDAQTAGIDGLLIVDLPPEESAPLRAALKDTGIQIILLAAPTTRPD
                     RLQMIAAKTEGFLYLITKAGTTGEGGLDYEAIRRHAAEVKAVCPLPVCLGFGIRDGSD
                     ARKLGPMADGIIVGSALVNITGSDPTATDLPARFAAKIRDLRTGLDTAP"
     gene            complement(9806..11074)
                     /gene="trpB"
                     /locus_tag="PKI64_05430"
     CDS             complement(9806..11074)
                     /gene="trpB"
                     /locus_tag="PKI64_05430"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE00488.1"
                     /translation="MGQYPDATGHFGPYGGRFVGETLMSALLDVEAAWNAAKTDSAFL
                     ADFRAMLRDYAGRPTPLDHAQRLSAHLGGAQIWLKREDCTHTGAHKINNVVGQGLLAR
                     RMGKTRVIAETGAGQHGVATATIAARFGFECKVFMGAEDIRRQAPNVRRMELLGAEVI
                     PVTSGTSTLKDAMNEALRYWSAHSTDTFYVIGSVAGPHPYPEMVRDFQRVIGDEARAQ
                     FFEKTGHHPDLLTACVGGGSNAMGLFYPYLDDPCEMIGAEAAGEGLHTSRHAASICGG
                     SPGELHGAMSYLLQSDEGQIHEAWSISAGLDYPGVGPEHALLHDLKRVKYEGVTDAEA
                     LAAFQMLCRHEGIIPALESSHALAVAIREAPKRPGQHILVNLSGRGDKDLDHVLRMLE
                     EVKAKGSGFGVQDSNLNSHLHHSEHRVSQR"
     gene            complement(11418..12479)
                     /locus_tag="PKI64_05435"
     CDS             complement(11418..12479)
                     /locus_tag="PKI64_05435"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE00489.1"
                     /translation="MSHPRLENINIESVLPLPPPREIKADLPLSTTAIATVGQGRETL
                     ERILDGEDPRLFAVVGPCSIHNLEAAEEYARKLKDLAEEVADTIYLVMRVYFEKPRSS
                     IGWKGLINDPYMNDTFHIEEGIRMARHFLIRVAELGLPAATELLDVLMPQYIGDLISW
                     NVIGARTAEAQTHREIASGISTPIGFKNGTDGSLEAAINSVRSAMGPHHFLGVTEDGL
                     PAVFSTTGNAYPHVVLRGGKKPNYDAESVAACQQALRDAHLPQNIMIDCSHGNSGKKP
                     ERQGLVLRDVLTQIENGAQCIRGFMLESNLEGGNQPMATNPARLNPRKSVTDACIDWP
                     TTEELIREAHTRLGKIIKK"
     assembly_gap    12599..12698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12699..13296)
                     /locus_tag="PKI64_05440"
     CDS             complement(<12699..13296)
                     /locus_tag="PKI64_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOE00490.1"
                     /translation="MFPFLKICGITRREDALFCAEAGAGALGAVFYPRSPRNVTAEQA
                     RDIFADLPADIARVGIFVNRATDEIILVARTATLTTVQLHGEESTADILALQSAGLHV
                     VKVLKTTGRDLLAYAQALPAGTGILVECGRGDLPGGNAAAWDWADAAILADNFSFGIA
                     GGIAPDNLAAAARASRAGGFDASSSLEAAPGIKDKASVL"
     gene            complement(13313..13708)
                     /locus_tag="PKI64_05445"
     CDS             complement(13313..13708)
                     /locus_tag="PKI64_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE00491.1"
                     /translation="MALDTNVLVRFLVRDDEAQARLAYQRLKQAEANRETLFVSLLVT
                     LETIWVLESAYRYSRNQILDALGALLTMSVLEFENMTVLQSLLAQARATTSDLSDLLI
                     ALHAQACGCAHTVTFDKSAAHHPLFQLLK"
     gene            complement(13708..13956)
                     /locus_tag="PKI64_05450"
     CDS             complement(13708..13956)
                     /locus_tag="PKI64_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOE00492.1"
                     /translation="MVTGTLTSKGQITIPKSIRDSLNLQTGDRLAFSLVGDKQALLQP
                     ITTSVDDVYGVLHRPGQKAKSIEDMNRSITRRHRQAKS"
     gene            complement(13978..14787)
                     /gene="trpC"
                     /locus_tag="PKI64_05455"
     CDS             complement(13978..14787)
                     /gene="trpC"
                     /locus_tag="PKI64_05455"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOE00493.1"
                     /translation="MSFLDKILAAKRAEIATAESHTPLATLKTLASRCTDFRDFTASL
                     NTPGIRIIAEIKRASPSLGDIAPDLSPATLARAYTTRGAAALSILTEPAFFKGSAADL
                     QQARAATTLPVLRKDFIIAPYQVYETAAMGADAMLLIVRILDDAQLRDLLALAHDLGL
                     HTLVEIHDEADARRLAGLNTPVIGINNRDLAHFHTDVNRAARLAALLPPETAVVAASG
                     IRDAHDIRQSLTNGIRRFLVGETLVKTPDSAALLREWTALDNSIPLRDSKE"
     gene            complement(15200..16216)
                     /gene="trpD"
                     /locus_tag="PKI64_05460"
     CDS             complement(15200..16216)
                     /gene="trpD"
                     /locus_tag="PKI64_05460"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOE00494.1"
                     /translation="MNIPSLIKKLIARQDLTEDESFAAITAMLSGTFTEGQIGAYLAT
                     LSAKGETIAEIVGAARAMRAAATRVQSLTPNTLDTVGTGGDGGITFNISTTVAFVAAG
                     AGATVAKHGNRASSGKSGSADCLECLGFNLNAAPELMEQALNEIGITFMFAPSFHKAM
                     RFAGPVRKQLGVRTLFNLLGPLTNPAGAPCQLLGVFAPELTETFAEVLKRLGSRRVLV
                     VHGHDGMDEITLTTTTRCSELKDGIIKTYDIDPAQYFGDYCTPEDLAGGEPADNAAIT
                     RGILEGKITGPKRDIVLLNAAASLLSANRCENLADGIQKAAAAIDTGTALDKLEQLIE
                     FSKQ"
     gene            complement(16720..17322)
                     /locus_tag="PKI64_05465"
     CDS             complement(16720..17322)
                     /locus_tag="PKI64_05465"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOE00495.1"
                     /translation="MILMLDNYDSFTYNLAQYLREMAADVRVVRNDAITTAEIESLAP
                     AAIVISPGPGRPESAGISVATVQAFAGKIPLLGVCLGHQAIGVAFGARVIHAQKLMHG
                     KVSEITCDGRGIFKGLDSRPFKAMRYHSLALDKATLPADLEISAAADDGEIMGIRHKQ
                     FPIEGIQFHPESIMTPVGKRILRNFLRTTENSEVRGQKSE"
     gene            complement(17359..>17699)
                     /locus_tag="PKI64_05470"
     CDS             complement(17359..>17699)
                     /locus_tag="PKI64_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOE00496.1"
                     /translation="DAFDLLASSFPAGTLSGAPKIRAMEIIADLEKEPRGPYGGAVGY
                     VSFDGNMDFCICIRTAVVEHGRLTIRTGAGIVADSDPQSELQETQNKAAAMTRALQLL
                     QTAQQTQPAP"
BASE COUNT         3348 a         4901 c         5818 g         3532 t
ORIGIN      
        1 cctgtgtccc aaggagttat gactttccat ttcagaggtg tcgaactcag gaaagatttc
       61 catgccgtgg aaaaatcggc ggtctttttt ccacagtgtg gaaaaaaagt ttccacagtg
      121 tggaaaacgg atcaatccgg gcaggcgccc ggagcatggc cgccatacaa gccgtgttca
      181 atccgttgct gctcgcgctg gatgacgccc tttagccagg ttgcctgctc gccgtcggtc
      241 gcgatgttct taaaatccac tttggtcgtg ttgagatatt ggaatgtcag tccataggcg
      301 aacggttgca tcggcagcca gccgcccagg aaaaatccga gctcctccgc cgccttcacc
      361 gcacccacga cattcggatc cgccaagtcc agcgtcagat aaatcacgtc cacccgcgca
      421 cggcagagcc ggcgcagctc gaagcccaat accgccgcga aatcggcgcc caccgccgga
      481 acatccagaa tcgcgatgtt gatggaggca atggtcgtgg tggcgaaggt ggattgcgcg
      541 ggcactgccg ccgccgcttc gtcgtctgct gtcgccgcga gttcggccag cgacaaatcc
      601 gatccggcca gcaaccgcgc gatggtgtcg cggtgccacg gcggcgggta caggcgttgc
      661 cgcggcaagg cccggaaaat gcgcgaggcc acaaagatgg tttcccgata ggccgcgttt
      721 tcctggatcc cgcgaaaacg gatgttgtcc acgataaagc cgagcagcag cgcggtgggc
      781 gcaaagccaa agcggttaca cgtcttttgc gtatagggat gactggtgac ggcgtgggca
      841 tacaggacgc tttcggttcg cgcacccatg gattcaattg cggcggccag cagctttttc
      901 atcagccctt cgctgcgcca cagggggtcc accaccaccg cgccccattc caccgcgggg
      961 gcggccggcg aatctttcat cagcgccaaa tgccccacca cgcgaccgct gggagtcacc
     1021 gcgccccatg atttgatgcg gcccgactgc tgatgcgccc gcacgcgctc tgggaaataa
     1081 aaatctgcat tggggtaggt ggagccatag ctgcggtaca tcaggcggca aaacccgatg
     1141 gcatccgctt ccgtcagcac gcgcagttgt ccgataggct cggggggggc ggcggcggct
     1201 tccgtttgcg ctgcgccggc caattccgtt gcttcttcgc cagcctgctc cgcaatgtgc
     1261 gcgcccggca aaaaccattc gagacaaaac tcgttgccct ccttgccgcg atactgaatc
     1321 tggcagcggt cggtggcctt ctttaataaa aacagcgcga ggccgctgct gaagtggtcc
     1381 tgccggattc cgccgcttgc cgccgcgctg tcgctgtccg ccgccaaacg ggaaagctgc
     1441 ccggcatcgt attccgggac ttcggacaga tcgaagggga gcccctcatt cacaatggaa
     1501 acccggagca ccggggcatc cactttcatc cggatgtcca gaaccgcgtc ggccacatcg
     1561 gcttcgtccg tgatgtaccg gaggatggtg gtgatgactt cttcgattgc gagttccatt
     1621 ggccgttggc gctctgccgg gaaaccgcat tccgccgcga ggttgcaggc ggcatcctga
     1681 acgatgcgca tccgctcgag cgatggcgga atccgcagat ggacccaggc gcacggcgga
     1741 ttcatgcctc gaacctccgg tccaccagac ggcgcagctt cccggtgcgc ggattccgca
     1801 acagcgtgtg cggctccacc cattcgatga ccagcggatg aatgatctgg ttgccgatgg
     1861 aatccggaaa catgggccgc tccgtataca gcgcgtcgcg gatcgcgtcc gccagcgcgg
     1921 ccggcatcgc gtccggacgc ggcaccgcaa tgcgcagggt gcagcggtcg cgctgttcaa
     1981 tatgatccac caccaattgg aagccggtat gtcccgtctg cgccgccagt cgcgaaagaa
     2041 tcgcctgaaa gtcttcgaga tagagcgtca ttgggccgac tcgcgcgcct ttttccgtcc
     2101 gccccagcaa ttgaaagcgg cgcggtttcc ccgtgaccgg ctccagccac atgcccatgt
     2161 cgccgaccgg ataccggata atgggcatga gcttccgctg gaagttggtc gcgtgaatcg
     2221 cacccggcac gcccggctcc tcgatcggcc ggttctcctc gtcgagaatt tccagcacga
     2281 tgctttcatc aaacacgtaa tgctctcccg ggccgcagct ttcatcgcac cagccgattt
     2341 cgccaaaatc cacgccggca attccgaccg actgcacccg gcacccggga tatgccgctt
     2401 gaacggcggc gatttgatcg ggatacatgc tctcgccggc aaacagcacg cggcgcaatt
     2461 caatgttttc gcgtccggcg cgaatcagct ccggcaaaaa atgcaggatg gtcgtgggca
     2521 ctcccgccaa ggtatgtatg cgaaactgga tcatggtatg aatcagttcc tcgggcggca
     2581 cgaatcccgc cagcggataa tgaacgccca cgccggattc ttcgagcgag cgcccgaaga
     2641 acagcagcga ggcatataac tgcccgccgt agaacagatt ggccacccgc tcgcccggcc
     2701 gcagcccgag ccgccgcaaa ccgccgccaa ataccccgca aaactcccgc caatcctcgt
     2761 tgctgaaaaa cgagaacttc ggatttcccg tcgtgccgcc gcttttgaac acgatccccg
     2821 aatcgtgcgg cgcggtcagc aggcggttgt ccacgatgct gttggcggcc cagtaacccg
     2881 cgtgatccgt caccggcaat gctgccaacc ccggacaggg cgcgccctcc ggccacgccc
     2941 cgcgccacgc ctccgcaaag tacggcgaat gcgcgcgcac aaattgcaga tgctcctcca
     3001 gtttccgtgt cgctgaattc atcgtgtcat ctccgttggt cgcaaatggg aatcactttt
     3061 ccgctcgcag gattccgctt gatttcggcg tcctccacca cgcgcacccg gtgccggaag
     3121 gccatcccca tgtcgtaggc atattggatt tcggcatagt tcaggcgtag caactcttcg
     3181 atttccgctg gcgtcggcgc gtccgggggg gcggtcagcc agacttccaa aactgtggtg
     3241 tgcccttctt ccgccaaatg gatttgcacg gggccggtgt gccccagttt cgtttccaga
     3301 atatccacaa accgccggta gttgaagaac ggtcccgcgg ccttgaagat gtcgccggtt
     3361 cggcccagca gctcgaagcg tgggtccgtt cggccgcacg ggcagggctc cggaacccag
     3421 cgaccggtgt cgccgatttc gtagcggttc acccgcggat attcccgtgt gatgggcgtc
     3481 agcagtagcc gtcccgtttc gcccggcgcc accggacggt cctcgtcgag cgccacaatc
     3541 tccatcttca ccagcgccga caacaaatgg tgctgcccac ccacgcagtg cgggcattga
     3601 tagcccattg ggcccgcgtc attcgagcca tagaccgccg agcgcacaat ggacacaccg
     3661 cagtccttca tcagaaacgc ccgctgcgcg actgtcattt tttcgccgcc gaagaacacc
     3721 ttctcgattt tgccgcgcag tcgcccggta caggcattga acagccccag caggtgcgat
     3781 ggcacgccca gcagcgtgtt cacgcggttg cttacgatga cgtcggcaat gtattcgtaa
     3841 tcgtcaatca tccccatcgg atactgcggc acgcgcaaaa attccagaat ggtccaaaag
     3901 ctgatcaaac tcccgtagag gtatccggcg gtatagcagt tcatcacgcg gtcgcgcgcc
     3961 gtttccaacc ccgccgccac caggccgtgc gcggtccaca tcatttgctg atgataatcc
     4021 gcccaagaat acagcgaata taccgtcttg ccgctcgatc ccccgctctt gaccaccagg
     4081 tccggacgct cgactgtttc cgccagtttc atgaaggcgt tcttgtcttg gatcggtccg
     4141 gtgggcgcgg acgcggcggc gcgcgtttcc agctcgttca gacagcccac gccttccgcc
     4201 tcggcggggc cgtccagcga tacgcgccgc gtgagctgct gcaacgcata gaccccatca
     4261 tgcggcgcgc cgatatagct gtccaccatt tcgcccgcgt ggcaaacccg cgtgaccccg
     4321 gcggcaaaca gcgcgcggct taaatcggcg agcgattcca atccgcacga caacccgcag
     4381 gtctgcagcc acgcgcgcat cgggcgcagc gtcgccgtca gctccgcccg tcggatcggc
     4441 ttgacccaaa tcgtgcgata caacggcgaa ggtcgcagcc ccgcacgcgc gtcaaacagc
     4501 acgcgccagc gcccctgcgc gtcctcgaaa agttccgtca atcccagcgc cttttcggcc
     4561 cgcgccaccg ccgtcaccga cgtgatttcc gcctgctcgc tggccgacgg cggctggcct
     4621 gggatggtcg gcgaaacctc cgccaggtgt gccgccagcc gcgccgcaat cttccgcgcg
     4681 gcatcctcgt cccggtccac ttccacaaac agcgtttgtg gacttgaaca cgcctgctgg
     4741 tccagccggc atacatccat cgccactgcc cgcagggtta cttccagttg tgcgtcgtcg
     4801 gtcaggcact cggcggcaag gtaggtaaag ctcaccttgt gtccccacga aatcagccgt
     4861 gtcccggacg gaatcgcttc acgaatggcg gcaattgccc gctcgccgcc ccacgccgaa
     4921 accccatcgg caatgccgaa cagccgcccc atgcgagcgg tgtcgccgga cgacagccgc
     4981 gccaccgcca cataatcccg caaccgcccg cctgggtcca gccggcacaa ttcctccgca
     5041 aacaccgccg caaactcggt gtcgcgcgcg ctcaccttta caatattcac attccccgcc
     5101 atcagacctt ccatcaggcc cagcaccggc gccgaaaata cgttcgacgg catcacatgc
     5161 accacgcagc ccagcgggaa ccacgcctcg aactgacgcc ccggataccg gcgtgctatc
     5221 acccccggcg gattcatccc caactcgctg cgcatttttt cctgcaccgc cccgcgcgcc
     5281 agcgtccccc gaatcgccgc aaacaagctt tccgcctcct cggccggggc cacgcgcaac
     5341 acaatctccc ggcaacgctc ataaaacgga ttgcccggaa ccagcgacgc cgcaatggca
     5401 tcgctcgcct ccaaaaaatc cgccatcgga aacggcatca gcagcacccg cgacaaatgc
     5461 gccgccagac ggctctcccc cgcaccgcgc tcctccagcc aggcgtccaa ccccgcgtca
     5521 tccagccact cgccaaacca aaaatgacgc cggagttccg ccgccgctcc cgtagatgct
     5581 ttcattcgtc cgcctttcgc ttctccgcca ccgtcaaatc tctgcgcctg cccctagcgt
     5641 cagcggtcag agtacccggc accttgtggc gggtaccctg gacccccttg ttcgaccatt
     5701 aatgggtttt tcatgcgtcg gatgtccgca ttgtctccta gcagattcca tgttcccgaa
     5761 tgcatgccaa ccacgatgtt cataggataa gcgtccttga gtccttttat catgcgcaag
     5821 tgtagcgacc gtgcggagcc gcccacaaat agacagggcg caagatggtg accctcttcc
     5881 gtcatcacat ccttcgggtc aatccgttct atccactcgt gaccggccat aagcatgcct
     5941 ataaacgaaa ccagaagctc cggaaaaggg accatgcttt tacattcgtg tgcagcaata
     6001 acgtcttttg agtctacgaa atagaatgtc cgttttccgc tagcatagtc gacgtccttc
     6061 cttttcccga tggttgctgc ttgaggagta acaacgatat caggcgtgaa agagatatcc
     6121 gttcccacat gcgatgagat tcgaacctgt tgtctgatct caatacactc ctcctcgcca
     6181 cgccgaagac gcatgtagga aaacttgtca ggattcccgt tgggtgtcgt gagatatcgg
     6241 taggaaccat cagttgccat attaacaacc tcgcctacaa acccagcttt ctcgtaaaac
     6301 tcgatgaggg caagtaagca tccaatctcg aagtgtgctg ctatctgacg ctctttcttt
     6361 gtgtaaacag tgccataagc ggttgcgaat tcccgcatcg cttctatgac ttggtcctcc
     6421 catgttggtg cagacgcgtc agtcattgtt tcctcgcgga tggccgagtt tgtataatag
     6481 gtcttgggcg tgagctgatt ggaggcacca gaagtctatt ccaagccgtt gaattactgc
     6541 ggagaactga ttatgacact cgcgaagtaa gtccgcatcc gcccccgtga ctcgcacctg
     6601 cttcccatag gcaattgggg tgtctcgcac caacccgact cggagatcgc tgcgcgatgc
     6661 ggcagcaatg gggccaagaa ctacttggta gaacgcgttt aggtcacaaa gaacgccaac
     6721 tctgtccacg ctccatcccg cgtctgctgc tgcacgatag ttacgtgact gcttttccgc
     6781 ttcgtaacgg attccgcgca ttaagctctc tgatcgagcg gtgatggact ggcgcgtgtc
     6841 gcggtcgaga ctggcagtca agtatttcag tgcttcgtgc ataaattatc taccaagtcg
     6901 aacagtgggt tttgagcagt tccgccgctg ccgccgcgca actgcggttc gccgatgtcc
     6961 ccgcccgccc cagaatctca aaccacgggg tggggtaggt gtcgagcgga cacgatcccg
     7021 ccggatgccg caccgccaaa tcccccatca ccacgctgtg cgccggcgtg gcgggaatat
     7081 acggcgacaa aaacgacaaa aatcccggcg tcccatccgg caccggggcc agcgttttca
     7141 aatcgcggat caccacgcgt gaccaggttg gttggtgcag gtggtgtcgc ggacagttcg
     7201 tgtacggcac gctgtgttcg accgaaccga atatctcaat gatccgctcc ggatctatac
     7261 caagctgccg ctcgatctgc gcaaaaaagt catccttcgc gatgctcttg tccgcgtagc
     7321 ccttccaacc gccgcccagg atcaacaggc ttcctggcgg caactttaaa tctgcctgcc
     7381 ctcgggcgcg ccgccgttcg agtaggaaat ataaatagta ggggaacccc agaatgcgca
     7441 ccggcgccga accttgtgcc catctttcga gacaggcgtc cgcgccaaac ggatcaaact
     7501 catgccggcc atccccctga tagcgcaacg tccagaattg ctccgccgcc ggcgcgtagc
     7561 tcatcaggta ggccatcgtg ttcgaggtgc ctaccttgag gcccgcgtac ggttcataga
     7621 aatttaacag ataattggct ggccgatcgt ccaacacgcc gcgccacacc atttcgtgtt
     7681 tagccatcac ccgcgcccac tccaaggtga agccgtcgaa aaacacctgc gatttctgtc
     7741 ccgtcgtccc tgacgacgtc aaatgagttt tgacctgcga ctcatgaatc gaacggattt
     7801 catgatactt gaaaaaattg gcgtgaaccg gcggcagcgc cagcacatcc gccattgtcc
     7861 ggatgcgctc cggcttcatc cccgcctgct ccgcaaaccg ccgataccac ggcgaccgtt
     7921 ccatgtgcag cgcggtgatg tcccgcatcg cctccacaaa cagcccgtca tatttctcgt
     7981 ctgctgcata cggcacggca atcgcgcaaa agcgccggac ggaatctgga atgtcgttgg
     8041 aaacgggagc ggacggttgg tccagcatta taactccaat cgaaatgatg gttttttgag
     8101 gacgttacca ccattgctga tgcggtggaa ccatttttcc ccgcccaaaa attgggggtt
     8161 taagggattg aagaaggggg cgggctgttc gtaagatggg gcggagagtt gaagaaagtg
     8221 cgcaatagag atgatttgat ggcgaagagt ggtcagaatg aacaaggcga gccgctcatc
     8281 ccgaagcatg gcggatttcg gcatctcaag ggcttccagg tagcgcaact ggtctatgat
     8341 gtcacggtgc ggttctgcga caaatacatt gatcggcgca gccgcaccca tgatcagatg
     8401 gtgcaggcgg cgcgctccgg tgtgcaaaac attgcggagg ggagcgtggc cagcggcacg
     8461 tccaagaaga tggagctgaa actgaccaat gtggccaggg cgagcttggc cgagctgcgc
     8521 cttgactacg aggattttct ccgtcagcgc gacctcaagc tctggccaca aaccgactcc
     8581 cgccgtcagg cgctggtggc gcggcgctgc gccaccgctg atgaggtggc ggcgtgggtg
     8641 gtggaggtgg cccaaaatgg agaaaatgga caggatggac ggaatggacg ggatggacat
     8701 ggaggaccgt ccatgcagtc catggagtcc atgagggctg ttctttttcc agaaatctcg
     8761 gccaatgcgg ctgttgttct gattggggtg gccatggcgt tgttggatcg tcagatccaa
     8821 gcgctggcgg cagcttttgc aaccgagggc ggttttaccg aacggcttta tcgtacccgg
     8881 cagtcaagcc gggcggagag ggggaaggag ccaaattagg gggcggtatc gaggccggtg
     8941 cggaggtcgc ggattttggc ggcgaagcgg gcggggaggt cggtggcggt tgggtcggag
     9001 ccggtgatgt tgacgagggc ggagccgacg atgatgccgt cggccatggg gccgagttta
     9061 cgagcgtcac tgccgtcgcg gatgccgaat ccgaggcaga cgggaagagg gcagacggct
     9121 ttgacttcgg cggcgtggcg acgaatggct tcgtagtcga gcccgccttc gccggtggtg
     9181 ccggctttgg tgatgaggta gaggaaacct tcggttttgg cggcgatcat ttggaggcga
     9241 tcgggccggg tggtgggggc ggcgaggagg atgatctgga tgccggtgtc ttttagagcg
     9301 gcgcggagag gggcggattc ttcggggggg aggtccacga tgaggaggcc atcaataccg
     9361 gcggtttggg cgtcggtggc gaggcgttca aggccgtatt tgtagatggg gttgtagtag
     9421 ctgaagagca tgagggggat gtcggtgtgg cggcggaggt cggcggcgag ttggagggtg
     9481 cgggagaggg tcatgccggt gcggagggcg cgctgggagg cggcttggat ggcagggccg
     9541 tcggcggtgg ggtcggagaa ggggacgccg agttcgagga tgtcgaggcc ggaggtggcg
     9601 gcggcgcgga ggattccgag gctggtggca tagtcgggat cgccggcggt gatgtagccg
     9661 atgagggctt tacggttctg ggtggcaagg cgttggaagg tttgggtgat tcggttcatg
     9721 gttatggagg gttcagggtt cagaggtcag gggaaagcaa aaacattttt tttaccacag
     9781 aggggcacag agaaaaatag atcattcacc tttgtgaaac tctgtgttcg ctgtggtgaa
     9841 ggtggctgtt caaattcgaa tcctgaaccc cgaaccctga acccttagct ttgacctctt
     9901 ccaacatgcg cagcacgtgg tcgaggtctt tgtcgccacg gcctgaaagg ttgacgagga
     9961 tgtgttggcc ggggcgtttg ggcgcttcgc ggatggcgac agcgagagca tgagagcttt
    10021 caagggcagg aataatcccc tcgtgccggc agagcatctg gaaagcggcg agggcttcgg
    10081 cgtcggtgac gccttcgtat ttcacgcgtt tgaggtcgtg gaggagggcg tgttcggggc
    10141 caacaccagg gtagtcgaga ccggcggaga tggaccaagc ttcgtggatt tggccttcgt
    10201 cggattggag gaggtaggac atggcaccgt ggagttcacc gggcgagccg ccgcagatgg
    10261 aggcggcgtg gcgagaggtg tggaggcctt cgccggcggc ttcggcgccg atcatttcgc
    10321 aggggtcgtc gaggtaggga tagaagaggc ccatagcgtt ggaccctccg ccgacgcagg
    10381 cggtgaggag gtcggggtgg tggccggttt tctcgaagaa ttgcgcgcgg gcttcgtcgc
    10441 cgatgacgcg ctggaagtcg cggaccattt cggggtaggg gtgggggccg gcgacgctgc
    10501 cgatgacgta aaaggtgtcg gtgctgtggg cggaccagta gcgcagggct tcgttcatgg
    10561 cgtctttgag cgtggaggtg ccggacgtga cggggatgac ttcggcgccg aggagttcca
    10621 tgcggcggac attgggggcc tgtcggcgga tgtcttcggc gcccatgaaa actttgcact
    10681 cgaagccgaa gcgggcggcg atggtggcgg tggccacgcc gtgctggccg gcgccggttt
    10741 cggcgatgac gcgtgttttg cccatgcggc gggcgaggag gccctggccg acgacgttgt
    10801 tgattttgtg ggcgccggtg tgggtgcagt cttcgcgctt gagccagatt tgcgcgccgc
    10861 cgaggtgggc gctgaggcgt tgagcgtggt cgaggggggt ggggcggccg gcgtagtcgc
    10921 ggagcatggc gcggaagtcg gccaagaagg cagagtcggt tttggcggca ttccaagcgg
    10981 cttcgacgtc gaggagggcg gacatgaggg tttcgccgac gaagcggccg ccgtaagggc
    11041 cgaagtggcc ggtagcgtca gggtattggc cgatgagttt tttcatgaga ggtcagggtt
    11101 cagaggtaag aggtcagggt tcaggagggg ggagaagcag aagaatggcg gtttctttgg
    11161 cgatttgtcg gcgcggaaac caaccgtcgc cttttccgtt ctggcgcgtg acaaccgacc
    11221 tctcgcgtac acgagtactg ctcgggtcgt ctgtcccgcg ccatcttcgg aaaaatcttg
    11281 gttatttccg cgaaacggcc ccaacaaatc accaaagaaa ccgccaatca ccacgtgctg
    11341 cgcacagcgg gcagggatgg gactgctgcg gcggagctgg cggaggggtg gcgtttggag
    11401 aggagaaagc atggtggtta cttcttgata attttaccga ggcgggtgtg ggcttcgcgg
    11461 atgagttctt cggtggtggg ccagtctatg caggcgtcgg tgacggattt gcgggggttg
    11521 aggcgtgccg ggttggtggc catgggctgg ttgccgcctt ccaaattgct ttcgagcatg
    11581 aacccgcgga tacattgcgc gccgttttcg atttgggtaa gcacgtcgcg cagtacaaga
    11641 ccctggcgct cgggcttctt gccggaattg ccatgcgagc agtcaatcat gatgttctgc
    11701 ggtagatggg catcgcgcag ggcttgttgg caggcggcaa cactctcggc gtcgtagttg
    11761 ggtttcttgc cgccacgcaa caccacgtgg ggataggcgt tgcccgtggt ggaaaacacg
    11821 gcgggcaggc catcttcggt gacaccgagg aaatggtgcg ggcccatagc ggagcggacg
    11881 ctgttgatgg cggcttccag cgagccgtcg gtgccgtttt tgaagccgat gggggtggag
    11941 atgccgctgg cgatttcgcg gtgagtctgg gcttcggccg tgcgcgcgcc aatgacgttc
    12001 catgaaatga ggtcgccgat gtactgcggc atgaggacgt cgagcagctc ggtggccgcc
    12061 ggcaggccca attccgccac gcggatcaag aagtgccgtg ccatgcggat gccttcttcg
    12121 atgtggaaag tgtcattcat gtaggggtcg ttgatcagcc ctttccagcc gatggaagag
    12181 cggggctttt caaaatagac gcgcatgacg agataaatcg tgtcggccac ttcctcggcg
    12241 aggtctttga gtttgcgggc gtattcttcc gcggcttcaa ggttgtggat ggaacaaggg
    12301 cccactaccg cgaacaggcg cgggtcctcg ccatcgagaa tgcgctccaa cgtttcgcgg
    12361 ccctggccca ccgtggcaat cgccgtagtg ctcaagggca agtccgcttt gatctctcgc
    12421 ggcggcggca gcgggaggac cgattcaata ttgatatttt ccaaccgggg gtggctcatg
    12481 ggggtccttt ccaaaaaaac ggttgtgccg gcagcgggag ttgagccgca gcctgcacat
    12541 aggccaaaac ggaggcttta tccttgattc caggtgcggc ttcaaggctg gaactggcnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aaaacggagg ctttatcctt
    12721 gattccaggt gcggcttcaa ggctggaact ggcgtcgaag ccacccgcac gggatgcacg
    12781 ggccgcagct gccagattgt ccggggcgat gccccccgca atgccgaacg aaaaattgtc
    12841 cgccaaaatg gcggcgtcgg cccaatccca ggcggcggcg ttgccgccgg gtagatcgcc
    12901 gcggccgcat tccaccagaa taccggtacc ggccggcaac gcctgcgcat aggccagtaa
    12961 atccctgcct gtagttttga gaaccttcac aacgtggagt cctgcgcttt gcagggcgag
    13021 aatgtccgcg gtggattcct cgccatggag ttggacggtg gttagcgtgg cggtgcgtgc
    13081 aaccaggata atctcatccg tggcacgatt tacgaaaatc cccacgcggg ctatgtcggc
    13141 cggcagatcg gcaaaaatat cgcgtgcctg ctcagcggtg acattgcgcg gactgcgcgg
    13201 ataaaacacc gcaccgagcg cgcccgcgcc ggcttcggca cagaagaggg catcttcgcg
    13261 ccgggtgatg ccgcagattt tgagaaaggg gaacatcgtc agaaaggttc ggttatttga
    13321 ggagttgaaa gaggggatga tgcgcggcgg acttgtcaaa ggtgacggta tgggcgcatc
    13381 cgcacgcctg ggcgtggagg gcgataagca gatcggagag gtccgaggtt gtcgcgcgcg
    13441 cttgcgcgag cagcgactgg aggacagtca tgttttcgaa ttcgagcacg ctcatggtga
    13501 gcagggctcc caacgcatcg agaatttgat tgcgggaata gcgataggca ctttccagaa
    13561 cccagatggt ttcgagggtg accagtaggg acacaaacag tgtttcgcgg ttggcctccg
    13621 cctgtttaag gcgttgatat gccaaacgtg cctgagcttc gtcgtcgcgc accagaaagc
    13681 gcacgagcac attggtatcc agcgccatca cgatttggcc tggcggtgcc gccgcgtaat
    13741 gctgcggttc atgtcttcaa tggattttgc cttttgtccg gggcggtgca gcacgccata
    13801 gacgtcatcc acgctggtcg tgatgggttg taggagggct tgtttgtcgc ccaccaagga
    13861 aaaggccaga cggtcgcccg tttgcagatt gagcgagtcg cggatactct tcgggatggt
    13921 gatttgcccc ttgctggtga gtgtgccggt gaccatgttt atctccttac ttatttccta
    13981 ttccttacta tctcgcaagg gaatggaatt gtcaagggcg gtccattcgc ggaggagggc
    14041 ggcagaatcg ggggttttga cgagggtttc gccgacaagg aagcggcgaa taccgttggt
    14101 gagggattgg cggatatcat gggcgtcgcg gatgccgctg gcagcgacaa cggcggtttc
    14161 cggcgggagg agcgcagcga ggcgggcggc gcggttgaca tcggtgtgaa aatgggcgag
    14221 gtcgcggttg ttgatgccga tgaccggggt attgaggccg gcgagacggc gggcgtcggc
    14281 ttcgtcgtgg atttcaacga gggtgtggag gccgaggtcg tgggcgaggg cgaggaggtc
    14341 gcggagttgg gcatcgtcga ggatgcggac gatgaggagc atggcgtcgg cgcccatggc
    14401 ggcagtttcg taaacctggt agggggcgat gatgaagtct ttgcgaagga cggggagggt
    14461 ggttgcggcg cgggcttgct ggaggtcggc ggcggagcct ttgaagaagg cgggttcggt
    14521 gaggatggag agggcagccg cgccgcgggt ggtgtaggcg cgggcgaggg tggcggggga
    14581 gaggtcgggg gcgatgtcgc cgagggaggg ggaggcgcgt ttgatttcgg cgatgatgcg
    14641 gatgccgggg gtgttgaggg aggcggtgaa gtcgcggaag tcggtgcacc gggaggcgag
    14701 ggttttgagg gtagcgaggg gggtgtggga ttcggcggtg gcgatttcgg cgcgtttggc
    14761 ggctaggatt ttgtcgagaa agctcatggt attgctacgg gattatgcgc ggggtggtgg
    14821 tggtgaagcg ggtggtgggg aggtggcagg tgctagggga ggcggaaaag gatttcacaa
    14881 aacgaggcgt caaaacgaag gcccgtcttt tttgttctgg cgcgtcgagg tcgcccactc
    14941 gcgtacacga gtactgctcg gggcgcccgc aacgcgccat ttccaaaaaa tccgagcgct
    15001 tattttgacg taacagcccc tcgttttgag aaatcctttt ccgcactgcc acgtgctgcg
    15061 cacagcgggc aagaagcgat gaagccattt cccgtgtggc tacgtgctgc gcacgagggg
    15121 tggggggaat ttttccacgg tgtagaaaaa agttttccac agtgtggaaa aagttggcgg
    15181 cggggcgggg catggcgggt tattgcttgg agaattcgat gagctgttcc agtttgtcga
    15241 gggcggtgcc ggtatcaatg gcggcggcgg ccttttggat gccgtcggcg aggttttcgc
    15301 agcggttggc ggagaggagg gaggcggcgg cgttgagcaa cacgatgtcg cgcttagggc
    15361 cggtgatttt gccttcgaga atcccgcggg tgatggcggc gttgtcggcg ggttcgccgc
    15421 cggcgaggtc ttcgggagtg cagtagtcgc cgaagtactg ggcgggatca atgtcgtagg
    15481 ttttgatgat gccgtccttc aattccgagc agcgggtggt ggtggtgagg gtgatttcgt
    15541 ccatgccgtc gtggccgtgg acaacaagga cgcggcggga gccgagtcgt ttgaggactt
    15601 cggcaaaggt ttcggtcagc tccggcgcga atacaccgag gagctggcag ggggcgccgg
    15661 cggggttggt gagggggccg agcaggttga agagggtgcg cacgccgagt tgcttgcgga
    15721 cggggccggc aaagcgcatg gccttgtgga acgagggggc gaacataaag gtgatgccga
    15781 tttcattgag cgcctgctcc atgagttcgg gcgccgcgtt aaggttgaag ccgaggcatt
    15841 cgaggcagtc agcgctgccg gatttgccgg agctggcgcg gttgccgtgt ttggcgacgg
    15901 tggcgccggc accggcggcg acgaaggcga cggtggtgga gatgttgaag gtgatgccgc
    15961 cgtcgccgcc ggtgccgacg gtgtcgaggg tgttgggggt gagagattgc acgcgggtgg
    16021 cggcggcgcg catggcgcgg gcggcgccga cgatttcggc gatggtttcg cctttggcgg
    16081 agagggtggc gaggtaggcg ccgatttggc cttcggtgaa ggtgccggag agcatggcgg
    16141 tgatggcggc gaaggattcg tcttcggtga ggtcctggcg ggcgataagt tttttgatga
    16201 ggctaggaat gttcatgtca agggctcagg gttcggggtt cagggttcag gggtgagagg
    16261 ttagagggca gggggcagtt gaggggcagc agaagaatgg cggtttcttt ggcgatttgt
    16321 cggcgcggaa accaaccgtc gccttttctg ttctggcgcg cgacaaccga cccctcgcgt
    16381 acacgagtac tgctcgggtc gtctgtcccg cgccatcttc gaaaaattct cggttatttc
    16441 cgcgtaaaag ccccgacaaa ttgtcaaaga aaccgccaac taccacgtgc tgcgcacggc
    16501 ggacaggatc attttgtaac atcctttgcc gcatggccac gtgctgcgca cagcggacac
    16561 tggtatttga taatgcacta aagaaattgc cgactgccgt gtgctgggtg cagtgagtgg
    16621 attgagtttt ccatggtgtg gaaaactttt ttccatagcg tggaaaatct gcggaaaagt
    16681 tttccacagt gtggaaaata ccggatgttc ggaggggact cattctgact tctggcctct
    16741 gacttctgaa ttctcggtag tgcgcaggaa atttcgcaaa atgcgtttgc cgacgggggt
    16801 catgatggat tcggggtgga attggatgcc ttcgatgggg aattgcttgt ggcggatgcc
    16861 catgatttcg ccatcgtcgg cggcggcgga gatttccaag tcggcgggca gggtggcttt
    16921 gtcgagggca agagaatgat agcgcatggc cttgaagggg cgggagtcga gtcccttgaa
    16981 aatgccgcgg ccgtcgcagg tgatttccga gaccttgccg tgcatcagtt tttgggcgtg
    17041 gatcacgcgg gcgccaaagg ccacgccgat ggcctgatgg ccgaggcaga cgcccagcag
    17101 cgggattttg ccggcgaagg cttgaacggt ggcaaccgaa atgccggcgg actcagggcg
    17161 gccggggccg ggcgaaatga caatggcggc gggggcgagg ctttcgattt cggcggtggt
    17221 gatggcatcg ttgcgcacca cgcggacatc cgcggccatc tcgcgcaggt attgggcgag
    17281 gttgtaggtg aaggagtcgt agttatcgag catcaagatc atggaagttt ccaggggaca
    17341 gggttcagag ttcagggttc agggggcggg ttgggtttgt tgggcggttt ggaggagttg
    17401 gagggcgcgg gtcatggcgg cggctttgtt ctgggtttcc tggagttctg attgcgggtc
    17461 ggagtcggca acaatgccgg cgccggtacg gatggtgagt cggccgtgtt ccacgactgc
    17521 agtgcgaata caaatgcaga agtccatatt gccgtcgaag gagacatagc cgactgcgcc
    17581 gccgtagggg ccgcgcggtt ctttttcgag gtcggcaatg atttccatgg cgcggatttt
    17641 gggcgcgccg gagagggtgc cggcggggaa ggaggaggcg agcagatcga aggcgtcgt
//
LOCUS       DAOPNP010000043        17608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3844_length_17608_cov_9.514385, whole genome shotgun sequence.
ACCESSION   DAOPNP010000043 DAOPNP010000000
VERSION     DAOPNP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17608
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_17608_cov_9.514385"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1708)
                     /gene="hcp"
                     /locus_tag="PKI64_05475"
                     /gene_synonym="priS"
     CDS             complement(155..1708)
                     /gene="hcp"
                     /locus_tag="PKI64_05475"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOE00497.1"
                     /translation="MSMFCFQCEQTVGGVACTKTGACGKTPEVANLQDEITAALITLA
                     RAVDGKPADPKIEALFMDALFMTVTNVNFDPQANTTMRDRILHAAVAAGGANSYRPED
                     LFNGDKDVVSLRSTLLFGMRGMAAYAAHARVLGKTDPKVSAWFQKGMKALGDEHSVEE
                     WLALIMEFGQVNLACMALLDSANTGAYGHPEPTRVSVTQHEGPFIIVTGHDLLDLRML
                     LEQSEGKGVNIYTHGEMLPAHGYPGLRKFKHLKGHFGTAWQNQQKEFANLPGVVLFTT
                     NCIMPPRPSYIQHLATTAEVGWPGVQHITADEKGHKDFSGIIQQAIALGGWPQTTPGA
                     YELMTGFAHQAVLAVADKVVAAVKSGAIKHIYLVGGCDGAKLGRDYYTEFVEKTGPDT
                     LVLTLACGKFRFNHLQDKLGTIGGIPRLLDMGQCNDAYSAIQVAVTLAKVFNCGVNDL
                     PLSLVLSWYEQKAVCILLTLLSLGIKDIRIGPTLPAFISPTVLNVLVEKFGLTPITTP
                     DADLQAIAG"
     gene            complement(1794..2531)
                     /locus_tag="PKI64_05480"
     CDS             complement(1794..2531)
                     /locus_tag="PKI64_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE00498.1"
                     /translation="MKRKIVEINEDLCNGCGLCVSACHEGAIQMVAGKARLVSDAYCD
                     GLGDCLPACPTGAIQITEREAAPFDAAAVAARQAAGQPAAKPADSALPPLPCGCPGQM
                     AKTIERKPTPPATAPAADAPTASELTNWPVQIKLVNVRAPYLQNAALLVAADCTAFAY
                     PDIHRRFMRNKVTLIGCPKLDEGDYAEKLTAILQANDIKSVTVLRMEVPCCGGITQAV
                     TRALQASGKMIPWQVVTVATDGTLLDE"
     gene            complement(2528..2965)
                     /locus_tag="PKI64_05485"
     CDS             complement(2528..2965)
                     /locus_tag="PKI64_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE00499.1"
                     /translation="MQGLLNIPAAVSIALHTCLWIADGDQPYYSSAEIARHLGFSYHH
                     VAKVVQRLVRAGILTAARGSKGGIRLARDPRRLSLLEIHLAAGGAPLVQHRCLLDPTI
                     CAGHACAFGHLLERENSRLYHKMKKTTLAQLARTFDRSQLELA"
     gene            3109..4416
                     /locus_tag="PKI64_05490"
     CDS             3109..4416
                     /locus_tag="PKI64_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE00500.1"
                     /translation="MKPVRGTPAQGLMGATVGFFVGFAAVSLFGPTVKHLQQAAGLSA
                     GLAGLLISIPNLTGSLLRIPFSAMCNRDGGRLAFLILLITAAVGLAGIWGLLAFVPDR
                     LGKLFPLLLVFGALGGAGIATFSVGISQTSFWYPQKTQGSALAAYAGIGNIAPGLFAL
                     LLGMLIPVIDLAYSYLAWLVFLLLGIAAYWVLGRNAPSFQLERAGLSRTEACAEARRH
                     HGQDIFPKGNTLHVLQESAKLWQTWALVFIYFTTFGGFMALTGWLPKYWSSCWGFDVA
                     RMGMLTALFSITASIIRIGGGPLADRLGGEKTALGSLALIALGAAVMTRAANVQQAVG
                     GIVLLALGMGVGNAATFKLVPQEVPHAIAGASGWVGGLGAFGGFVIPNVLAAFLTNGD
                     AGDRGYATGYWTFAVLAAAAALLVLAMHASRLRRERTARFVDS"
     gene            4427..6526
                     /locus_tag="PKI64_05495"
     CDS             4427..6526
                     /locus_tag="PKI64_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HOE00501.1"
                     /translation="MTQTIRTTCSYCSVGCNFDATLNDQGVVTKFMPNATYPVNAGKA
                     CPKGFHLLTPFKAADRLTTPLRRAADGSWQAIGWDAALDEFVTKLQSIKTQHGPESVA
                     FLSTGQLPLEEMAFLGALFKFGMGFLHCDGNTRQCMATAVVAYKQAFGFDAPPFSYED
                     LEQSDLMIFVGANPAVAHPVLWNRIKANDTGGDMIVLDPRRTQTAEAATVHVPLKAKS
                     DIVLLYGIARLLIERGWIDRDFIAAHTEGFAEFAAHVAAFTLERTAAATGLGITTIED
                     LAERIHRAKAMSVWWTMGVNQGHQAVRTAQAIINLCLITGHIGRPGTGPNSLTGQANA
                     MGSRLFSNTTAMFALHEFTNPEHRAKMAELLRMPLHVIPEKNSLPYHRIMEEIDRGKI
                     KALWVVCTNPAHSWIGKAPAIAALKKLDFLVVQDLYSTTDTAQLAHLLLPAAGSGEKN
                     GTFINSERRFGIVQKVMDPPGEALPDFEIFRRIADRWGCGKQFAEWTDPEAVFRILQR
                     TTAGRPCDITGIQGYAMLRERPGIQWPYPQEGADDSDHRRLFADGRFFTPSGRAKLLF
                     DPITSPPEEPDVNYPFLLLTGRGSIGQWHTQTRTGKVDMINKISPAELPIQVSPDDAA
                     RLRLAKGDRVLVKSRRGQTEAVVVVTNEVSPGHVFMSMHYYGTNLLTYPVFDPYSFQP
                     GYKYAAVSIERVPAPTP"
     gene            6523..7155
                     /locus_tag="PKI64_05500"
     CDS             6523..7155
                     /locus_tag="PKI64_05500"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOE00502.1"
                     /translation="MNPAPPAARSTPAEDVIGVVLAGGFGRRMGRDKSLVPLAGQPAA
                     QRQAALLREATRRVVVSVRPEQLSGKDRAAFNGLECIADDTPGAGPLGALVTVARHFP
                     ERALLIVAVDLAGLTASALRQLLAARAPEVAVVAARQQPAGISSTTSDLPHPLCALWT
                     PPALAAARRAWQAGSRSPRWVLADIACDNPRAIRLLAIPFTDRNTPPPDA"
     gene            7197..7272
                     /locus_tag="PKI64_05505"
     tRNA            7197..7272
                     /locus_tag="PKI64_05505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7230..7232,aa:Thr,seq:cgt)
     gene            complement(7279..7977)
                     /locus_tag="PKI64_05510"
     CDS             complement(7279..7977)
                     /locus_tag="PKI64_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00503.1"
                     /translation="MSNDILIAGRNLQRHYRRGSEIVRALDGVDLEIRAGESVCILGP
                     SGSGKTTLVNLLSALDVPTAGELEIGGRRVDGLSEAALVDVRRGMLGYVFQQFHLIPT
                     LTVMENIALPLLFLRRKVDPAELLRVLEAVGLADRAKHLPKELSGGQMQRVAIGRALV
                     VRPRVLIADEPTGNLDSHTGEAIFTLFQKTVREDGVALVLTTHNLALAARCDRTLTLT
                     DGKIVGTTAAGARG"
     gene            complement(7980..9227)
                     /locus_tag="PKI64_05515"
     CDS             complement(7980..9227)
                     /locus_tag="PKI64_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00504.1"
                     /translation="MNLTSFAWKNLTRRPVRTMLTAGGVGLAVAVAVSLGGFNLGYRQ
                     AIAGSIEQLGFQVMVMAKGCPYEAATMMLKGGSGLLYLPEEAHEQIRTDPDIEDITPI
                     FIGIAEKQNYSLDGTPQASAYTIMSGVELDSFVTMKPWLKFKHGPGYDGGRWFAPTAT
                     NEVVMGYEVAEYEQRKVGDTFYATVVPNGKVEPELHAFTVVGVLDRTGAQDDGTVFLP
                     LPAAQAIFGRPGELTIIGIKLKEFNGIRMREFEGRWLKIPEVQVVSLEQVKGALVSLV
                     GTAQTMVAAVALIAVLVAMIGVVNAILMSVHERTSELGVLKAIGAGRAQIFRLVWLET
                     LLISLAGAVPGSLVATAGARLTDLALRKVLNIGVTQSLVRITPGLLGVVIVGAVVLSL
                     LAGIWPAGRAANLRPVEAIRSGE"
     gene            complement(9224..9625)
                     /locus_tag="PKI64_05520"
     CDS             complement(9224..9625)
                     /locus_tag="PKI64_05520"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HOE00505.1"
                     /translation="MKTSQLRWMILALLVGVGAGMIGCARQKAEHFGPAFTDAPRVTI
                     AQLLADPEAYRRQTVRVAGDIERQCPSSGCWMFLRDEEGRSIRVELGDYFAELPRHVG
                     QRAEVEGEWIPHGTGHQFIGTRVTFGGEAAP"
     gene            complement(9622..10320)
                     /locus_tag="PKI64_05525"
     CDS             complement(9622..10320)
                     /locus_tag="PKI64_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00506.1"
                     /translation="MKRILGLGLAGGVLAASAAWGAIGCTLSNPARDLQTLFPEMTSY
                     REDVKEFGKLPQGRADYAALQERVGGDLDPVYEAFDTPYTLYSVFKGDERIGYVHGVN
                     VPGRGGVIQVFLAVEPESGGIRRMFYQRLESPGGPALRNPAVTGQFAGLTLADFYKHD
                     YFAAVDPANEADTVGRIAPPAQLPDAARPDWEATLRGVRKDLILLDIFVFERRHDPFY
                     ERAVAARQGKENTP"
     gene            complement(10335..11453)
                     /locus_tag="PKI64_05530"
     CDS             complement(10335..11453)
                     /locus_tag="PKI64_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00507.1"
                     /translation="MMNRAVSLGLLLAGAAIAADDPFADVRPQMAPAATEPAIPWWRD
                     NWQVRRELYGVVAGGWHDLDDTGRASVRLSAGFEVQKRLATATRTLGAVDYQGRVVYR
                     EHSLNATGDAMGMDAARWTYETHNAYFELYNVAGELGRLNLRAGRFYVPFGLSWQTDT
                     HGTLLQLANERVFGDSHDWQVTAYGNATELLDYQVGYVFGAGPRQHWDGQSGMAVGRL
                     GLNNTARNERGREGGISAAYGQRLDPAVGAGVVDTWRVGVDARQRLDTGGGPITLTGE
                     GAAGEDDQRAVWSGLLQADWLHPSRRWGAAAQYTYFTRRAATPATQQSAALVATRYFR
                     NEVGNTALHWLAVGVENRWAATDMPDDTLLLAQYYHYW"
     gene            complement(11450..11716)
                     /locus_tag="PKI64_05535"
     CDS             complement(11450..11716)
                     /locus_tag="PKI64_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00508.1"
                     /translation="MKLNLILILLVMATTVAWAAGHPQTHCPVMTANEVDATSPYVDV
                     DGYRIYICCRGCAKAIQDDPAKYIAQLQAEGVELEKAPEAPAAE"
     gene            complement(11743..12027)
                     /locus_tag="PKI64_05540"
     CDS             complement(11743..12027)
                     /locus_tag="PKI64_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOE00509.1"
                     /translation="MKKQLEHPAHDDVQPRLRRIAGQIQGVQRMIEQRKYCCDILTQI
                     QAARSALRAVELKILEKHMTHCVNSALVSQSPADATEKVAELLEILKRRC"
     gene            complement(12152..12631)
                     /locus_tag="PKI64_05545"
     CDS             complement(12152..12631)
                     /locus_tag="PKI64_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOE00510.1"
                     /translation="MKTGERERQILVVCTGNTCRSPMAAGWLSKKLAGKGWVVQSAGV
                     AAWPGQPAAAEAIEAMREIGCDISGHRTRAVETKMVADARYILVMTAGHQREIVARFP
                     LAENKVFLVHGFGVQEACDVSDPVGMPLETYRRTRDEIVQALGDFLLYLAENGEISH"
     gene            complement(12666..14228)
                     /gene="proS"
                     /locus_tag="PKI64_05550"
     CDS             complement(12666..14228)
                     /gene="proS"
                     /locus_tag="PKI64_05550"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOE00511.1"
                     /translation="MSAQPNQKQQPATAADGITPRAVDYGQWYLDIVKRADLADHSSV
                     RGCMVIKPHGYAIWEKLQRELDDRFKATGHVNAYFPLFIPLSFLAKEEEHAAGFAKEC
                     AVVTHYRLKSLDGKVGVDPESKLEEPLVVRPTSETIIWAQYKNWIQSYRDLPLLINQW
                     ANVVRWEMRTRLFLRTAEFLWQEGHTAHATEAEAREEVSRMLAVYADVAENVMGVPVV
                     RGHKTEGERFAGALDTQCIEGMMQDGKALQMGTSHYLGQNFAKSADVKFLNKDGQLEY
                     VHATSWGVSTRLIGALIMTHSDDNGLILPPKLAPIHVVIVPIYKSAEEQARTTAEAQA
                     VATELRAQGWSVKVDDREGLQPGAKYYEWERKGVPVRIEVGPKDLEKGSLCVVRRFVL
                     EKDGESEQELRKRKKQFLPRAEAIANMPTLLATLQKELLERARVMRDKRTRIIDTLEE
                     YAEFFKGDGGFAWVHWAGTRDDEDTMAKRFETSIRCLPFADQIPEAGRGPGKCILTGQ
                     PSAQRVLMAKAY"
     gene            complement(14306..14686)
                     /locus_tag="PKI64_05555"
     CDS             complement(14306..14686)
                     /locus_tag="PKI64_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOE00512.1"
                     /translation="MRENEVSRAVYESAIEVHRALGGPGLLEGVYEEALAWELATQGL
                     KVERQVQVPIQYKGHTLGTPLRLDLLVEGCVVVECKATAEYNPIFESQVLTYLRISGL
                     KLGLVINFGEKIVKEGIHRVVNGL"
     gene            complement(14849..16108)
                     /gene="icd"
                     /locus_tag="PKI64_05560"
     CDS             complement(14849..16108)
                     /gene="icd"
                     /locus_tag="PKI64_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOE00513.1"
                     /translation="MSESKIKVPADGQKITMGASGLAVPARPIIPYIEGDGIGPDITR
                     AALTVWNAAVAKAYGGERRVAWMEIYAGEKANAVYGPNTWLPTETLAACRDFLVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPVRWFTGVPSPVKHPEKTDMVIFRENSEDI
                     YAGIEWAAGTPEVQKVVAFLQQEMGVKKIRFPGSSGIGIKPVSEEGTRRLVRAAIRYA
                     LANGRRSVTLVHKGNIMKFTEGAFRDWGYATARDEFGAQPLDGGPWHVLKDPATGREI
                     VVKDVIADAFLQQILTRPAEYDVIATLNLNGDYISDALAACVGGIGIAPGANINYDSG
                     CAVFEATHGTAPKYANLDKVNPGSLILSGEMMFRYLGWTEAADLIIKGVEKTIGQKVV
                     TYDFARLMAGAQEVSCSAFGNAIAENM"
     regulatory      16500..16617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            16678..>17608
                     /locus_tag="PKI64_05565"
     CDS             16678..>17608
                     /locus_tag="PKI64_05565"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOE00514.1"
                     /translation="MRRWLFGLLVLWVARAWAAQTIVSFATNFTAPGQFINDRPLADG
                     PAFFGNTFTDWGGGFTSWAGFAFSTVSNTTDGSFGNQYAAAAPHSNAYAVAYDDPWNP
                     PPVIAFDIPIHPRSVQINNTTYAALTIRNGSGFSRPFTDGDYFVLTLTARDLENRIVA
                     TTNHYLADFRDGKSFIQTNWTTLDLSWMPPSVATLAGTLETTDMGAFGANTPMYFALA
                     DFTYAYAGLADGLAATNPAIVCWADAVTDYTPGANVDAQWKNAAHALGPAEMGDGFNG
                     STNVVSLGDGGHITLTFPLPITDGPGPDFAVFEN"
BASE COUNT         3139 a         5993 c         5078 g         3398 t
ORIGIN      
        1 gtgcggcttt gctaactaca taaagtgtga aggtttgctt tcaccacgac accatgaaga
       61 tttacgcgct tttaacaaaa cgttgacggc acaaacaaag cggcccgggc gggattacca
      121 caccgggccc gtgatgattt ttcgaagggt gtttttaacc ggcgatggcc tgcaaatccg
      181 cgtccggagt cgtaatgggc gtgaggccga acttctcgac gaggacgttg agcacggtgg
      241 gcgaaatgaa cgccggcagc gtgggcccga tgcggatgtc cttgatgccg agcgagagca
      301 aggtcaacag gatgcacacc gccttttgct cataccatga aagcacgagc gagaggggca
      361 gatcgttcac gccgcagttg aatactttgg cgagggtgac ggccacttga atggcggaat
      421 aggcatcatt gcattgcccc atgtcgagca ggcgcggaat gccgccaatg gtcccgagct
      481 tgtcctgcag gtggttgaag cggaacttgc cgcaggccag cgtgagcaca agcgtgtcgg
      541 gcccggtttt ttccacaaac tcggtgtagt agtcgcgacc cagtttggcg ccgtcgcaac
      601 cgccgacgag ataaatgtgt ttgatggcgc cgctcttgac ggccgccacc actttgtcgg
      661 ccacggccag cacggcctga tgggcaaagc cggtcatcag ttcataggcg ccgggggtgg
      721 tttgcggcca gccgccgagc gcaatggctt gttgaataat gccggagaag tctttgtgac
      781 ccttttcgtc agccgtgatg tgctgcacgc cgggccagcc cacctccgcc gtggtggcca
      841 gatgctggat ataggacggg cgcggcggca tgatgcaatt ggtggtgaaa agcaccacgc
      901 cggggagatt ggcgaattcc ttctgctggt tctgccacgc ggtaccaaaa tggcccttga
      961 ggtgtttgaa tttgcgcagg ccggggtagc catgggcagg cagcatttcg ccgtgggtat
     1021 aaatgttgac gcccttgccc tcggactgct ccaacagcat ccgcaggtcc agcagatcgt
     1081 gaccggtgac gatgatgaac gggccctcgt gctgcgtgac ggatacgcgc gtcggttccg
     1141 ggtggccata ggcgccggtg ttggcggagt cgagcagcgc catgcaggcc agattcactt
     1201 ggccgaactc catgatcagc gccagccact cttccacgct gtgctcatcg cccagcgcct
     1261 tcatgccctt ctggaaccag gcggaaacct tgggatcggt cttgccgagc acgcgggcgt
     1321 gggcggcata ggccgccatg ccgcgcatgc cgaacagcag cgtcgagcgc agcgagacca
     1381 catccttatc gccattgaac aggtcctcgg gccggtagga gttggccccg ccggcggcca
     1441 ccgccgcgtg caggatgcgg tcgcgcatgg tggtgttggc ctgcgggtca aagttgacgt
     1501 tggtgacggt catgaacagc gcatccatga aaagcgcctc gatcttggga tcggccggct
     1561 tgccatccac cgcgcgggcc agcgtgatta gcgctgcggt gatttcgtct tgcaggttgg
     1621 ctacttcggg ggttttgccg caagcgccgg tcttggtgca ggccacgccg cccacggtct
     1681 gttcgcattg gaaacaaaac atgctcattg gttgggttct cctcggggtt ctttttttac
     1741 actggttgca acagggttgg taaatcaggc gctagttgtg gcccaccagc cggctattcg
     1801 tcaagcaggg tgccatcggt cgccacggtc acgacctgcc acgggatcat cttgccggaa
     1861 gcttgcagcg cgcgggtcac ggcctgggtg atgccgccgc agcagggcac ttccatgcgc
     1921 agcacggtga cgctcttgat gtcgttggcc tgcaggatgg ccgtcaattt ttcggcatag
     1981 tcgccctcgt ccagcttggg gcaaccgatc agcgtgacct tgttgcgcat gaagcggcgg
     2041 tggatgtcgg gataggcaaa ggcggtgcaa tccgccgcca ccagcagcgc cgcgttctgc
     2101 agataaggcg cgcggacgtt gaccagcttg atttgcaccg gccagttggt cagctccgat
     2161 gccgttgggg catccgcggc gggcgcagta gcgggcggcg tcggcttgcg ctcgatggtc
     2221 ttggccattt gccccgggca gccgcaaggc agcggtggca acgcggagtc cgcaggtttg
     2281 gccgccggct ggccggccgc ctgccgagcg gcaaccgccg cggcatcgaa gggcgcagct
     2341 tcgcgctcgg taatctggat ggcgccagtg gggcacgcgg gcaggcaatc gcccagcccg
     2401 tcgcaatagg catcggacac cagccgggcc ttgccggcca ccatctggat ggcgccttcg
     2461 tggcaggcgc tcacgcacag gccgcagccg ttgcagaggt cttcgttgat ttcgacaatt
     2521 tttcgtttca tgccagctcc aattgggatc ggtcaaacgt acgcgcgagt tgcgcgagcg
     2581 tggtcttttt catcttgtgg tataggcggc tgttttcgcg ttcgagcaag tggccgaagg
     2641 cgcaggcgtg cccggcgcag atggtggggt ccagcaggca gcggtgctgt accagtggtg
     2701 ccccaccggc ggccagatga atctccagca gcgacagtcg ccgcgggtcg cgcgcgaggc
     2761 ggatgccgcc tttggacccg cgggccgccg tgagaatgcc ggcccgcacc agccgctgca
     2821 ccaccttggc cacgtggtga taggaaaacc ccaagtgccg cgcgatctcg gcggaggaat
     2881 aatagggctg gtcgccatcg gcaatccaca aacaggtatg gagcgcgatg gaaaccgccg
     2941 ccgggatgtt caaaagtcct tgcatgggcg ccacgttcgc gatattggta tcgcaatacc
     3001 aaattgctgt caaggcccgc gcgcgatttg ttttttcgga aacggattgc tttccggggc
     3061 atggctgcgc acgatcaaac gaaacatggc aggagcaacg gacaaacgat gaaaccagta
     3121 cggggaaccc ccgcacaggg gttgatgggc gcaacggtcg gattctttgt gggctttgcg
     3181 gcggtctcgc tgttcgggcc cacggtcaaa catttgcagc aggccgccgg cttgtcggcg
     3241 gggctggcgg ggttgttgat ttccattccc aacctgacgg gatcgctgct gcggattcca
     3301 ttttcggcca tgtgcaaccg cgacggcggg cggctggcct ttctgatttt gctgatcacc
     3361 gcggccgtcg gcttggcggg catttggggc ctgctggcgt ttgtgcccga tcgcctcggc
     3421 aagctcttcc cgctgctgct ggtgtttggc gcgctgggcg gcgcggggat cgcgacgttc
     3481 agcgtgggca tcagccagac ttcgttttgg tatccgcaaa aaacgcaggg ctccgcgctg
     3541 gcggcctacg cgggcattgg caatattgcc ccggggctat tcgcgctcct gctgggaatg
     3601 ttgattccgg tgattgactt ggcgtactcc tatctggcgt ggctggtgtt tctgctgctg
     3661 ggcatcgcgg cctactgggt gttgggccgc aacgcgcctt ccttccaact ggagcgcgcg
     3721 ggcctctcgc gcacggaagc ctgcgccgag gctcgtcgtc accacggcca ggatattttt
     3781 ccaaagggca acacgctgca cgtactgcag gaatccgcca agctctggca gacatgggcg
     3841 ctggtgttca tctatttcac tacgttcggc ggcttcatgg cgctgaccgg ttggctgccg
     3901 aaatactgga gttcctgctg gggctttgat gtcgcgcgca tggggatgct gaccgcactg
     3961 ttttccatta cggccagcat catccgcatt ggcggcgggc cgctggccga ccggctgggc
     4021 ggcgaaaaaa ccgcactggg ctcgctggcg ctgattgcgc taggcgcggc ggtcatgacc
     4081 cgcgccgcca acgttcagca ggccgtcggc ggcattgtgc tgctggcgct ggggatgggc
     4141 gtgggcaacg cggccacgtt caaactggtg ccgcaggaag tgccgcacgc cattgcgggc
     4201 gcatcgggct gggtgggcgg ccttggcgcc ttcggtggtt ttgtgattcc caatgtgttg
     4261 gccgccttcc taaccaacgg tgatgccggc gaccgcggct atgccaccgg ctattggaca
     4321 tttgccgtgc tggccgccgc ggccgcacta ttggtgctgg ccatgcacgc cagccgcctg
     4381 cgccgcgagc gcaccgcccg atttgttgac tcatgaggag cctgtcatga cccagaccat
     4441 ccgcaccact tgcagctatt gttcggttgg ctgcaacttt gatgccacgc tcaatgacca
     4501 aggcgtggta accaagttca tgccgaacgc cacctatccc gtcaatgccg gcaaggcatg
     4561 ccccaagggg ttccacctgc tcacgccgtt caaggcggcg gaccgcctga ccacaccgct
     4621 gcgccgcgcg gcggacggct cgtggcaggc catcggctgg gatgccgcgc tggatgagtt
     4681 tgtgaccaaa ctgcaatcca tcaagacgca gcacgggccg gaatcggtgg cgtttttatc
     4741 caccggccag ctcccgctcg aggagatggc gttcctcggt gcgctgttca agtttgggat
     4801 gggctttctc cattgtgacg gcaacacccg gcagtgcatg gccaccgccg tggtggccta
     4861 taaacaggca ttcgggttcg atgccccccc cttctcctac gaagaccttg aacaatctga
     4921 cctgatgatt tttgtgggcg ccaaccccgc agtggcccac cccgtgctgt ggaaccgcat
     4981 caaagccaac gataccggtg gcgacatgat tgtgctggac ccgcgccgga cgcaaacagc
     5041 cgaagccgcc accgtccatg tgccgctcaa ggccaagagc gacattgtcc tgctctacgg
     5101 cattgcccgg ctgctgatcg agcgcggctg gattgaccgc gacttcatcg ctgcgcacac
     5161 ggagggcttt gcggaatttg cggcgcacgt ggctgcgttc acgctggagc gcaccgccgc
     5221 cgccaccgga ctgggcatca ccaccatcga ggacctcgcc gaacggattc accgcgccaa
     5281 ggccatgtcg gtctggtgga ccatgggggt aaatcaaggc caccaagccg tgcgtaccgc
     5341 gcaggcgatc atcaacctct gcctgatcac cggccatatt ggccgtccgg gcaccggccc
     5401 caattcgctg acgggccagg ccaacgcgat gggctcgcgg ctgttctcca acaccaccgc
     5461 gatgtttgcg ctgcacgaat tcaccaaccc cgaacaccgc gcaaaaatgg cggagctgct
     5521 ccgcatgccg ctgcacgtca tccccgaaaa aaacagcctg ccctatcacc gcatcatgga
     5581 agaaattgac cggggtaaaa tcaaggcgct ctgggtggtg tgcaccaatc cggcgcactc
     5641 gtggatcggc aaagcgccgg ccatcgccgc gctcaaaaag ctcgatttcc tcgtggtgca
     5701 ggatttgtat tccaccaccg acaccgccca acttgcgcac ctgctgcttc ccgccgccgg
     5761 cagcggcgaa aagaacggta ccttcatcaa ttccgagcgc cgtttcggca tcgtgcaaaa
     5821 agtcatggac ccgcccggcg aggcacttcc cgacttcgag atcttccggc gtatcgccga
     5881 ccgctggggc tgtggcaaac aattcgcgga atggacggac cccgaagcgg tctttcgcat
     5941 tttgcaacgc accaccgccg gccgcccctg cgacatcacc ggcatccaag gctacgccat
     6001 gctccgcgag cggcccggca tccagtggcc ctacccccag gaaggcgccg acgattccga
     6061 ccaccgccgc ctgtttgccg acggtcgctt cttcacgccc tccggccgcg ccaagctgct
     6121 gtttgatccc atcacctcgc cgccggaaga accggatgtc aattatccgt tcctgctgct
     6181 gaccgggcgc ggcagcattg gccagtggca cacccagacg cgcacgggca aggtggacat
     6241 gatcaacaag atttcgcccg ccgaactgcc catccaagtc tcgcccgatg acgccgcccg
     6301 cctgcgcttg gccaagggtg accgcgtact ggtgaaatcg cgccgcggtc aaaccgaggc
     6361 cgtggtggtg gtgaccaacg aggtctcccc cggccatgtc tttatgtcca tgcactatta
     6421 cggcaccaac ctgctcacct atcccgtgtt cgacccctac agcttccagc cgggctacaa
     6481 atatgccgcg gtcagcatcg agcgggtgcc ggcgccgact ccgtgaaccc cgcgccacca
     6541 gccgcccgct ccactcccgc ggaggatgtc atcggcgtgg tgttggccgg cggctttggc
     6601 cggcgaatgg ggcgcgacaa gagccttgtg ccgctggccg gccaaccggc cgcgcaacgg
     6661 caggccgcac tgttgcgcga agccacccgc cgcgtggtgg tgtcggtgcg ccccgagcaa
     6721 ctcagcggca aagaccgtgc cgctttcaac ggcttggagt gcatcgcgga tgacaccccc
     6781 ggcgccggtc cgttgggcgc gctcgtcacc gtggcgcggc attttccaga acgcgcgctg
     6841 ctgattgtgg ccgttgattt ggccggactc accgcttccg cgctgcgtca actgcttgcg
     6901 gcgcgcgcgc cagaggtggc ggtggtggcc gcgcggcagc aacccgccgg tatttcctca
     6961 acgacaagcg atttgcccca cccgctttgc gccttgtgga ccccgcccgc cctggcggca
     7021 gcacgccgcg catggcaagc cgggtcgcgt agtccgcggt gggtactggc cgacatcgcg
     7081 tgcgacaacc cccgcgctat ccggctgctg gccatcccgt tcaccgaccg caatacaccg
     7141 ccgccggatg cttgactctc cgcgcgggcc tttggcatag tgcggccctc gttgacgcca
     7201 acgtagctca gctggcagag caccgcattc gtaatgcgac ggtcgtcggt tcgaatccga
     7261 ccgttggctc cactcgccct acccccgcgc ccccgccgcc gtggtcccca cgattttgcc
     7321 gtcggtcagc gtcagtgtgc ggtcgcaacg cgctgccagc gccaagttgt gggtggtcag
     7381 caccagcgcc acgccatcct cacgcaccgt tttttggaac agcgtaaaaa tggcctcgcc
     7441 cgtgtggctg tccaagttgc ctgtcggttc gtcggcaatc agtacgcgcg ggcgcaccac
     7501 cagtgcgcgg ccgatggcca cgcgctgcat ttggccgccg gacaattcct tgggcaggtg
     7561 cttggcgcgg tcggccaaac ccaccgcctc cagcacgcgc agcagctcgg cggggtccac
     7621 cttgcgccgc aaaaacagta gcggcagcgc gatgttttcc atgaccgtca acgtgggaat
     7681 caggtggaac tgttggaata catagcccag catcccgcgg cgcacgtcca cgagcgcggc
     7741 ctcgctcagg ccgtccaccc ggcgcccacc aatctccaat tcgccggcgg tgggcacatc
     7801 caacgcggaa agcagattga ccagcgtggt tttgccggag cccgacggcc ccaggatgca
     7861 caccgattcg cccgcgcgga tttccaaatc cacgccatcg agcgcgcgca caatttcgct
     7921 gccgcgccgg taatgccgct gtaagttgcg gcccgcaatc aaaatgtcat tgctcatgat
     7981 tattctccac tccggatggc ttccacgggg cgcaagttgg cggcgcggcc agccggccaa
     8041 attccggcca gcaagctcag caccaccgcg cccacaatca ccacgcccag caggcccggc
     8101 gtaatgcgca ccagcgattg cgtcacgccg atgttcagca ccttgcgcag cgcgaggtcc
     8161 gtcaaccgcg cgccagcggt ggccaccagg ctgcccggga ccgcgccggc cagactgatc
     8221 agtaacgtct ccagccatac caggcggaag atttgcgcgc ggcctgcgcc gatggccttc
     8281 aacaccccga gctcgctggt gcgctcatgc acgctcatca ggatggcatt caccacgcca
     8341 atcatggcta ccagcaccgc aatcagcgcc accgccgcca ccatggtttg cgccgtgccc
     8401 accagcgaca cgagcgcgcc cttcacctgc tccaggctga ccacctgtac ctcgggaatc
     8461 ttgagccagc ggccctcgaa ttcacgcatc cggatgccgt tgaattcctt gagcttgatg
     8521 ccgatgatcg tcagctcgcc cggccgcccg aagatcgcct gcgccgccgg cagcggcaga
     8581 aaaaccgtgc cgtcatcctg cgcgccggtt cgatccagca cgcccaccac cgtgaacgca
     8641 tggagctccg gctccacttt gccgttgggc accaccgtgg cgtagaacgt gtcgccgact
     8701 ttgcgctgtt catactccgc cacttcgtag cccatcacca cctcgttggt ggccgtgggc
     8761 gcgaaccagc gtccgccgtc atagccgggg ccgtgcttga acttcagcca cggcttcatc
     8821 gtcacaaaac tatccagctc cacgccgctc atgatcgtgt aggccgaggc ctgcggtgtg
     8881 ccatccagcg aataattttg cttttcggca ataccgataa aaatcggcgt gatgtcttca
     8941 atgtcgggat cggtgcggat ttgctcgtgc gcctcttccg gcagatagag caatcccgaa
     9001 ccgcctttaa gcatcatggt cgcggcctca taggggcagc ccttggccat gaccatgact
     9061 tgaaatccaa gctgctcgat actgccggcg atggcctgcc gatagccgag attgaatccg
     9121 ccaaggctca ccgccaccgc caccgccaga cccacgcccc ccgccgtcag catggtgcgc
     9181 acgggacggc gcgtcagatt tttccaggca aacgaggtca gattcatggc gcggcctccc
     9241 caccaaacgt gacccgcgtg ccgatgaact gatgccccgt gccgtgcgga atccactcgc
     9301 cttccacttc ggcgcgctgg ccgacgtgcc gcggcagctc cgcaaagtag tcgcccaatt
     9361 ccacgcggat cgagcggcct tcctcatcac gcaaaaacat ccagcagccg gaggacggac
     9421 attgccgttc gatatcgccc gccacgcgca ccgtttgccg gcggtaggcc tccgggtccg
     9481 ccagtaattg agcaatcgtc acgcgcggcg catccgtgaa cgcggggccg aaatgctccg
     9541 ctttctggcg ggcgcaaccg atcatgcccg cgccgacgcc caccaacagg gccaaaatca
     9601 tccagcgtag ttgagaagtt ttcatggggt attttccttt ccttgccggg cggcgaccgc
     9661 gcgctcatag aagggatcgt gccgccgctc aaaaacaaaa atgtcgagaa ggatcaggtc
     9721 cttgcgcacg ccgcgcagcg tggcctccca atccggccgg gccgcgtccg gcaactgcgc
     9781 gggcggcgca atccggccga cagtatccgc ttcgttggcg gggtccaccg ccgcgaaata
     9841 atcgtgctta tagaaatccg ccagcgtcag gccggcgaac tgtcccgtca ccgctgggtt
     9901 gcgcaacgcg gggccgccgg gactttccaa acgctggtaa aacatccggc ggatgccccc
     9961 cgattccggc tccaccgcca ggaacacttg aatgacccca ccgcgtccgg gcacgttcac
    10021 gccgtggaca tagccgatac gctcgtcgcc cttgaacacg gagtagagtg tgtagggcgt
    10081 gtcgaacgcc tcatacaccg gatccaaatc gccgcccacc cgctcttgca gcgcggcata
    10141 atcggcgcgg ccctgcggca gcttgccgaa ctctttcaca tcctcgcgat acgacgtcat
    10201 ctcgggaaac agcgtttgca aatcgcgcgc cggattgctt aacgtgcagc cgatggctcc
    10261 ccacgcggcc gaagccgcca gcaccccgcc cgccagtccc agtcccagaa tccgtttcat
    10321 gttcagcctc gctttcacca atagtggtaa tactgcgcca gtaacagcgt gtcgtccggc
    10381 atgtccgtgg ccgcccagcg gttctcgacg cccaccgcca gccaatgcag cgcggtgttg
    10441 ccaacctcgt tgcggaaata acgcgtggcc accagcgcgg cgctttgctg cgtggccggg
    10501 gtggccgcac gccgcgtaaa ataggtatat tgcgccgccg cgccccagcg gcgcgacgga
    10561 tgcagccaat ccgcttgcag cagccccgac cacaccgcgc gctggtcatc ctcgccggcc
    10621 gcgccttcgc cggtcagcgt gatgggcccg ccgccggtgt ccagtcgttg ccgcgcatcc
    10681 acccccacgc gccacgtatc caccaccccc gccccgaccg ccggatccag ccgctggcca
    10741 tacgccgcgc tgatcccgcc ttcgcgcccg cgctcgttgc gcgccgtgtt gttcaagccc
    10801 agccgcccaa cggccatccc gctttgcccg tcccaatgct ggcgcggccc cgcgccgaac
    10861 acgtagccaa cttgataatc cagcagctcc gtcgcgttgc cgtaggcggt gacttgccaa
    10921 tcgtggctgt cgccgaacac gcgctcgttg gccagttgca gcaacgtgcc gtgagtgtcg
    10981 gtttgccagc tcaagccaaa cggcacataa aaacggcccg cgcgcaaatt gagccgccca
    11041 agttcgccgg ccacgttata caactcaaaa taggcgttgt gcgtttcata agtccagcgg
    11101 gcggcatcca ttcccatggc gtcgccggtt gcgttcaagc tgtgctcgcg atacaccacg
    11161 cgcccctgat aatccactgc gccgagcgtc cgcgtggctg tcgccaaccg tttttgcact
    11221 tcaaaccccg ccgagaggcg cacgctggcg cgcccggtgt catccaagtc atgccagcca
    11281 ccggcaacca cgccatacag ctcgcggcgg acttgccaat tatcccgcca ccacggaatc
    11341 gccggctccg tggcagcggg cgccatctgc gggcgcacat cggcaaaggg atcatcggcc
    11401 gcaatggccg ccccagccaa gagcaacccg agactgacgg cacgattcat cattccgcgg
    11461 cgggtgcctc tggcgccttt tccagctcca caccttccgc ctgcaactgc gcgatatact
    11521 tcgccgggtc atcttggatg gccttggcgc agccccggca gcagatataa atccggtagc
    11581 cgtccacatc cacatagggc gatgtggcat ccacctcgtt cgccgtcatc acggggcagt
    11641 gagtttgggg atggcccgca gcccacgcca cggtggttgc catcaccaac aaaatcagga
    11701 tcagattcag tttcatgtgt ttcctttctt gaggaactct cctcaacagc gtcgttttaa
    11761 tatctccaac aattccgcca ccttctcggt ggcatccgcg ggcgattgcg acaccagcgc
    11821 ggagttgacg caatgcgtca tgtgcttttc cagaatcttc agttccacgg cccgcagcgc
    11881 cgagcgcgcg gcctgaatct gggtgaggat gtcgcagcaa tatttgcgct gctcgatcat
    11941 tcgttgcacc ccctgaatct ggccggcaat acgccgcaag cggggttgaa cgtcatcgtg
    12001 ggcgggatgt tccagttgtt ttttcatacc cccaacatac ccccaagggg tatttacgca
    12061 agtaatccaa ttggttttct tggatttgca acgggaaaat aacgtatcta ctggcaacaa
    12121 aaaggattgc ggattttatg cagccgagaa tttagtgcga aatctcgccg ttttcagcca
    12181 gatatagcaa gaaatccccc agcgcctgca cgatttcgtc gcgggtccgc cgatacgtct
    12241 ccagcggcat gcccaccggg tcgctcacat cgcaggcctc ctgcacgccg aagccatgaa
    12301 ccagaaacac cttgttttcg gccagtggga accgcgccac gatttcccgc tggtgccccg
    12361 cggtcatcac cagaatgtag cgcgcgtcgg ccaccatttt ggtctccacc gcgcgggtgc
    12421 ggtgccccga aatatcgcag ccgatttccc gcatggcctc aatggcctcc gccgctgccg
    12481 gctgccccgg ccacgccgcc acccccgccg actgcaccac ccaccccttg cccgccaact
    12541 ttttgctcaa ccagccagcg gccattggac tccggcacgt attccccgtg caaaccacca
    12601 gaatctgccg ctcacgttca ccggtcttca tccgcgctcc gttccggctt ggcgggccaa
    12661 gccgctcaat aggctttggc catcaatacc cgttgcgcgc tcggctggcc agtcaaaata
    12721 cacttgcccg gtccgcgccc cgcctccgga atttggtcgg caaacggcag gcagcggata
    12781 ctggtttcaa agcgcttggc catggtgtcc tcgtcgtcgc gcgtgccggc ccaatgcacc
    12841 caggcaaagc cgccatcgcc cttgaagaac tctgcgtact cctccagcgt gtcaatgatg
    12901 cgcgtgcgct tgtcgcgcat gacacgcgcc cgctccagca attccttttg cagcgtcgcc
    12961 agcagcgtgg gcatgttggc gatggcctcc gcgcgcggca aaaactgttt cttgcgcttg
    13021 cgcagctcct gctcgctctc gccgtccttc tccagcacga accggcgcac cacgcacagc
    13081 gaaccttttt cgaggtcctt gggacccacc tcaatccgca ccggcacgcc cttgcgttcc
    13141 cactcgtaat atttcgcacc cggctgcaac ccttcgcggt cgtccacctt cacgctccag
    13201 ccctgcgcgc gcagctccgt ggccaccgcc tgcgcctcgg ccgttgtccg cgcctgctcc
    13261 tccgcgctct tgtagatcgg cacaatcacc acatgaatgg gtgccagttt cggcggtaga
    13321 atcagcccgt tgtcatccga gtgcgtcatg atcagcgcgc caatcagccg cgtcgagacc
    13381 ccccaactgg tcgcgtggac atattccaac tggccgtcct tgttcaaaaa cttcacgtcg
    13441 gcgctcttgg caaagttttg gcccagatag tggctggtgc ccatttgcag cgccttgccg
    13501 tcctgcatca tgccctcgat gcactgcgta tccagcgcgc cggcaaaccg ctcgccctcg
    13561 gtcttgtggc cccgcaccac cggcacgccc atcacgtttt ccgccacatc ggcatagacc
    13621 gccagcatgc gcgacacctc ctcacgcgcc tctgcttccg tcgcgtgcgc ggtatgtccc
    13681 tcctgccaca gaaactccgc cgtgcgcagg aacagacgcg tacgcatttc ccagcgcacc
    13741 acattcgccc actggttgat cagcagcggc aaatcacgat agctctggat ccaattcttg
    13801 tattgcgccc aaatgattgt ttcgctcgtg ggccgcacca ccagcggctc ttccagtttc
    13861 gactccgggt ccacccccac cttgccatcc agcgacttca gccggtaatg cgtcaccacc
    13921 gcgcactcct tggcgaaccc cgccgcgtgc tcctcttcct tcgccaaaaa cgacagcggg
    13981 ataaacagcg gaaagtacgc attcacgtgc cccgtcgcct tgaagcggtc atccagctcg
    14041 cgctgcaact tttcccagat ggcgtaaccg tgcggcttga tcaccatgca cccgcgcacc
    14101 gacgaatgat ccgccaggtc cgcgcgcttc acgatgtcca gataccactg cccataatca
    14161 accgctcgcg gcgtgatccc gtccgccgcc gtcgccggct gctgcttctg gttcggctgt
    14221 gcactcatct caactctcct tcactacaaa aatccattta acgcaaacga cgccaacgac
    14281 gccaaaggcc gccagcgccg aaccgtcaca agccatttac cacccggtga atgccctctt
    14341 tcacgatctt ttctccaaaa ttgatgacca aacccaactt cagcccggat attctcaaat
    14401 acgtcaaaac ttgggactca aaaatcggat tatattccgc cgttgccttg cactccacca
    14461 ctacgcatcc ttcaaccagc aaatccagcc gtaacggcgt ccccagcgtg tgccctttat
    14521 attgaatggg cacttgaacc tggcgctcaa ccttcaggcc ttgcgttgcc aactcccacg
    14581 ccaatgcctc ctcatacaca ccctccaaca aacccggccc gcccagcgcc cggtgtactt
    14641 caatcgcgct ctcgtacacc gcccggctga cctcattctc cctcatcctt catcctgtct
    14701 ggcgtttgcg cccttttgcg cctcttgcgt cttttgcgtt aaatggctgc tcggctgttc
    14761 ggttctctga caccttgcac cgtctccgtt tgcgctcttt tgcgtctctt gcgtcgcttg
    14821 cgttaaatgg ctgttctggc tgttctcctc acatgttctc cgcaatcgcg ttgccgaagg
    14881 ccgagcaact cacttcctgc gccccggcca tcagccgcgc gaagtcgtac gtcaccacct
    14941 tctgcccgat ggtcttctcc acgcccttga tgatcaaatc cgcggcctcg gtccagccca
    15001 gatagcggaa catcatttcg ccgctgagaa tcagcgagcc gggattcacc ttgtcgaggt
    15061 tggcatactt cggcgcggtg ccgtgcgtgg cctcgaaaac cgcgcagccg ctgtcataat
    15121 taatgttggc gccgggcgcg atgccgatac cgcccacgca cgccgccagc gcgtcggaaa
    15181 tataatcgcc gttgaggttc agcgtggcaa tcacgtcata ctccgccggg cgcgtcagaa
    15241 tctgctgcaa aaacgcgtcg gctatcacgt ccttcaccac gatctcgcgg ccggtcgccg
    15301 ggtccttcag cacgtgccac gggccgccat ccagcggctg cgcgccgaac tcgtcgcgcg
    15361 ccgtggcgta gccccagtcg cggaacgcgc cttcggtaaa tttcatgatg ttgcccttgt
    15421 gcaccagcgt gacgctccgg cgcccgttgg ccagcgcgta gcggatcgcc gcgcgcacca
    15481 gccgccgcgt cccctcctcg ctcaccggct tgatgccgat gcccgagctg cccggaaaac
    15541 ggattttctt cacgcccatt tcctgctgca aaaaggccac caccttctgc acctccggcg
    15601 tccccgccgc ccactcaatc cccgcgtaaa tgtcttccga gttctcgcgg aaaatcacca
    15661 tgtccgtctt ttccggatgc ttgacggggc tgggcacgcc cgtgaaccag cgcaccggcc
    15721 gcaggcacac atacaaatcc agctcctgcc gcagcgcgac gttcaaactc cggatgcccc
    15781 cgcccaccgg cgtcgtcagc ggccccttga tggacaccag aaaatcgcgg cacgccgcca
    15841 gcgtctcggt cggtagccac gtattgggcc catagaccgc attcgccttt tcgcccgcgt
    15901 aaatctccat ccatgccacg cggcgctctc cgccatacgc cttggccacc gccgcgttcc
    15961 acaccgtcag cgccgcgcgc gtgatgtccg gcccgatgcc atcgccctcg atgtacggaa
    16021 taatcggccg cgccggcacc gccagcccgc tcgcgcccat cgtgattttc tgcccgtccg
    16081 ccgggacctt gatcttgctc tcgctcatcg tctcgtcctt tcccaaacat cctgttcagc
    16141 ccgccggggc gggtttttcc acagtgtgga aaaataattt ccacagcgtg gaaaatttca
    16201 ccaaagtttt tccattgtgt ggaaaaatcg cgcaaagttt ttccattgcg tggaaaaatc
    16261 cgcgaaactt tttccatagt gtggaaaaat ccagccgttt ttttccacag tgtggaaaac
    16321 ttttcatccc gccggggcgg aattttccac agtgtggaaa aacgcggggc cacttttcca
    16381 cagtgtgaaa aaaaggtcaa aacaaaccct tgcgcgcgga tttggcgctt gtgcacggca
    16441 ggggcgcagt gctaggttgc ggggcacgtc gccagggtgg cggcgtctcc ggcgactacg
    16501 gaagcgggtg agattcccgc gcggtcgcgc cgctgtgaac ggttaggaaa ttccaacagg
    16561 ccattccggc cctcagccgg gagaaggtgg aagagtaggg gccgtaagcc agaagacggg
    16621 aagccggggc aagtgcaact ccctgccctc gctgattggg gcggaaagga tggcgccatg
    16681 cggcgttggt tgtttggtct gttggttctg tgggtggcgc gcgcgtgggc ggcgcagacg
    16741 atcgtttcgt tcgcaaccaa tttcactgct ccggggcagt tcatcaacga ccgcccgctc
    16801 gccgacggcc cagcattttt cggcaacacg ttcacggact gggggggcgg cttcacgtcg
    16861 tgggctggct tcgcgttttc caccgtcagc aacaccaccg atggctcctt cggcaaccaa
    16921 tatgccgccg ccgcgccgca cagcaacgcc tacgccgtgg cctatgacga tccttggaat
    16981 ccgccgccgg tcatcgcgtt cgacattccc atccaccccc gctccgtcca gatcaacaac
    17041 accacctacg ccgctctcac catccgcaat ggatcgggct ttagccggcc cttcaccgat
    17101 ggcgattatt ttgtgctgac tctgaccgcg cgcgatctgg agaaccgcat cgtcgccacc
    17161 accaatcatt atctggcgga tttccgcgat ggcaaaagtt tcatccaaac caactggacc
    17221 acgctggacc tctcctggat gccgccgtcc gtggccacgc tggccggcac gctggagacc
    17281 accgacatgg gtgcgttcgg cgcaaatact ccgatgtatt ttgcgctggc ggacttcacc
    17341 tatgcctacg ccggcctcgc ggatggcctc gccgccacca accccgccat tgtctgctgg
    17401 gcagatgccg tgaccgacta cacccccggc gccaatgtgg acgcgcaatg gaaaaatgcc
    17461 gcccacgccc tcggacccgc cgaaatgggc gacggcttca acggctccac caatgtcgtg
    17521 agcctcggcg acggcggcca catcacattg acattcccgc tgcccatcac cgacggcccc
    17581 ggtccggact tcgctgtttt tgaaaatg
//
LOCUS       DAOPNP010000044        17519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3868_length_17519_cov_8.279146, whole genome shotgun sequence.
ACCESSION   DAOPNP010000044 DAOPNP010000000
VERSION     DAOPNP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17519
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_17519_cov_8.279146"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKI64_05570"
     CDS             <1..468
                     /locus_tag="PKI64_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00515.1"
                     /translation="IREWRDLLPRYLQFWQEGGRGLAFVFFEECARRFPNDGLALNNM
                     AWLLATAEPDGLRHERMAEWPATALVWAERALAQGGSDMPGAWDTLAAARANAGDFAG
                     AVAAVERARELAKQAGEWTLDVQLQARLAEYRAGRPWREPDARAKTTGSKPIP"
     gene            465..2579
                     /locus_tag="PKI64_05575"
     CDS             465..2579
                     /locus_tag="PKI64_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00516.1"
                     /translation="MKADIREKLRLAGIGLLIAVALFAVYAPSIGYDFILLDDGPYVQ
                     NNPTVSGGFSWPAVKAAWTEANEAYWAPLLWMSFMLDVEWFGLNPHGFHLVNVVLFSL
                     NAGLLFALFRRWTGRTGVAVAATLLWALHPARVESVAWVVERKDVLSGLFFLLGLGAY
                     VEGRQSRLPAGVGLAWLCMALGGAVKQILIVMPAVLMLLDVWPLARTDWDRIGRDGWR
                     LAREKWTFWGLAAFLAFLPPWLHHQDGAMMDVPISHRLAMIPIHYLFYLKKSLWPSGL
                     AVLQGDLPFRWPAFALGIGLLAGATWGLWRVRKAAPWALWGWAWFVGALFPLSGLVWA
                     GAERLATRFLYVPHMGLALAVVLGLAHRGRNRRVIAAAAALLVLAVYLAATVRLLPYW
                     RDSRTLFTRVLQLNPDSVHGFDNLARAYFDEGRFAEWQAYLEDFRARPSGSGIADIQY
                     AWWQAAMLGDAETAWATLEKRMGLPRTDPDFWLWLETRTNDRMLLGTWRDVAGICLRT
                     AGDLERLEQMWQHGENRWDERTRMNFLAEMLYAHWSAGREAEADALAREAGSLSGALL
                     RAQMPARFAERWQQGARGYAFACFQNYARWVPGDGLALNNMAWLVATAKPDGLRHARM
                     DEWPAAAVEWAEQALAASGGSIPAVWGTLAAARANAGDFAGAQAAADRALALAEAAGE
                     RSLAAKFQAQREEYRAGRPWRE"
     gene            2624..4069
                     /locus_tag="PKI64_05580"
     CDS             2624..4069
                     /locus_tag="PKI64_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00517.1"
                     /translation="MKRMVFLALAWSLGATGSGAADVVSNAMSGVRYPSLATAVAAAT
                     SGDRLVMIGNETLGATLIVSNKTLTLVSDGTVRTVTGSTNCTDGMVSILGVTATLTLG
                     QPEGSDAAPTLVFDGGRYQGVTSLSNMFFSDLGWLTIHPGVVLRNLASVDVGAIYNLS
                     GVVEMYGGRIENNSAPYGGGIFNQTGDIWIYGGSITGNVAQIGGGIYNDAVQWFMGYI
                     VGYYGKVDVRGGLIANNAANVAGGGILSYGALDLSGGQVLRNSAPQGGGVLHANGKAP
                     YGMSLRGTAVVASNSATQGSGIYYNDDGNTWLTLAEGGRVEPSNDVFLAIGTSPVILE
                     SALTGRGLAARLTPAAYATNQIVLGASTNSNLWIVSNYYGKFTVTPAPGDPEPWYVNV
                     NGRLSRDNSAATPATILSIASSETNVEWGVEPAFQAWDGVVELATNLVGQAWNFQPLA
                     PEAYSVTNGRVVVPRDRPLGIYRLRTGGAGP"
     gene            complement(4447..5157)
                     /locus_tag="PKI64_05585"
     CDS             complement(4447..5157)
                     /locus_tag="PKI64_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE00518.1"
                     /translation="MKTTLFIPTRNEIEAMQVIMPQIDRSWVDQILVADYSTDGTADY
                     ARSQGYDVIEQKGKGIRWAYIEAFPHVRGDVVITFSPDGNSPVRSIPLLIEEMKKGYD
                     MVIASRYLPPAKSEDDDPMTAFGNWLFTHSINLLHGHSWRRPYTDAMVIYRAYRTQLF
                     YELDLHKEESYTPEKWFGTVLGIEPLLSIRAAKRKLKIGEIPEDEPPRIGGERKLQPF
                     RWGAAYMAQVWRELWFWK"
     gene            complement(5195..6868)
                     /gene="recN"
                     /locus_tag="PKI64_05590"
     CDS             complement(5195..6868)
                     /gene="recN"
                     /locus_tag="PKI64_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOE00519.1"
                     /translation="MLTLLRVKNLALVEEATVAFEPGLNIITGETGAGKSILIGALYL
                     LLGERADTSAIRAGETQCVVEATFQLAEPQVVNDILTDTGLEPCDHGLLILRRTVKAA
                     GGGQAFVNDAPVTLPLLKRLGAHLVDLHGPHDHQSLFQPASQLDILDSFARADAERAA
                     YANAFSRRQALLARREELAGDRGDLDAQIALYAHQVREIEEAAPVEGEEDEVRAEQQT
                     AGHAQRILELGQLIVQATSEDENSALAIMAPARKALDELARLLPAAKEWLAEFRGHTD
                     ALAALTGAIQSELTHISADPARLEWLDQRLATYDKLKRKYGPSLADILQTLAAAKTRL
                     QDLQTRDQQKADVEKELLAVEKELQTAGRALRKRRAAAAEKLAPAITAELQALGFAHG
                     LFAIELTPSEAPLASGLDKLEFAFAPNVGEPMRPLRAIASSGEISRVMLGIKVVVAAA
                     DRIPLLVFDEIDANVGGETARAVGQKLAQAAKRRQVIAITHLPTVAACGAHHHAVRKE
                     VQAGRTHTRVVALDHAARVEELARMLGGRESTSVTLRHAAELLATTPTS"
     gene            complement(6933..7637)
                     /locus_tag="PKI64_05595"
     CDS             complement(6933..7637)
                     /locus_tag="PKI64_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOE00520.1"
                     /translation="MKHVFFGLLLLMVAITGARAQDIRTWTSASGATIEAAFVSQQAD
                     VVILQKPDGEKLRIRLNQLSPADQRFLGERRTAGLNRKSTADAEDAPIPPALQDLVGR
                     KLVNAKGHRVSPATLAGKKIGLYFSASWCPPCHAFTPVLVKAYNEMQTASKPFEVVLI
                     CLDRSEADMRRYMDEFNMPWLAIPFGDKVGNALQSKFRVNGIPTLIIVDDAGQTIAAD
                     GRGQITSRGAAAFDSW"
     gene            7778..9649
                     /gene="asnB"
                     /locus_tag="PKI64_05600"
     CDS             7778..9649
                     /gene="asnB"
                     /locus_tag="PKI64_05600"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOE00521.1"
                     /translation="MSGICGFTWRDFNLGQRMASVMAYRGPDHEGVWCDDHMTLAHRR
                     LDVMDLSAAGNQPVASADGQKHLVFDGEIYNLAAVRQELAAKGRVPAEAASAVVLLGA
                     YEEWGPAMVHKLVGSFAIALWDAARRRLWLVRDRLGIKPLYYYFKDARLVFGSEIKSI
                     LEDAQIPRRVNHAALYAYLGFEFIPAPDTAFENIRKLPAGHWATLENGQLRVEEYWDL
                     RMETPATPLGEGEVVERIRALIDEAVREQLVSSRPLGAFLSGGLDSSTLVAMMHKHSA
                     GKVRTFTIGFPDKTFSELNYAQTVADHFGTEHQVLIIDDMDEAALEKSIWHLDEPMTD
                     LSAVPLMFLCEKAKAHISVCLSGEGGDEVFVGYDRYKAAKIARFLNVLPRWVRHNLIG
                     KWTASLKDRPEKKGPVNMFKRFMEGALLPEDGEHVRWQYFMNARFEQQLFTAGYKAQV
                     AFDPFARVRAHSAKCATRDKVNLHAYLDTRFMMPDGVLMKADKLSLASSLLLRVPLLD
                     HRLVEYMGTVPGHFKLRGLTTKYIFRKALEGILPKEIIWRGKQGYSLPVKNLLRNQFK
                     DYMTTLLHESPLIREDFELAFVDQLIAEHLNMQHNHNHVLWGLMNIAIWHRRYFPHG"
     gene            9662..10405
                     /locus_tag="PKI64_05605"
     CDS             9662..10405
                     /locus_tag="PKI64_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE00522.1"
                     /translation="MTTGAEYKVKEVTQGSAAGAYRALMYGDMSLGKVIWAEILFTLL
                     GGLGGAVGLFLRMKLYPTLFKSCGRKVVFGRNVTIRHPHKIVLGDGVVLDDNCLVDAK
                     GAGNTGIILHERVYVGRNTLIYCKNGDIEIGANVSLSANCVIFSANQITLQPNSVVAA
                     FVYLLSGGEYDLADPTPFAFQKGTCTKGPLTIGADCWIGAGAKVLDGASLGDRVVLGA
                     GAVATKPVPAHSIAVGVPARVIKTRQVPG"
     gene            10402..11343
                     /locus_tag="PKI64_05610"
     CDS             10402..11343
                     /locus_tag="PKI64_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOE00523.1"
                     /translation="MKKRLTLLGKLLVMSGLLALLYRKADWASFWGALTDLQWGWLPL
                     IYALLLFNTAISAWKWQQLLRADDVRVPLRSLLVSYLIGTFFNLFLPSSIGGDSYRVV
                     DASRHGGAAKSFASVFADRLTGFLALAIWGLVFSAIGWSRLPDKRILWLPVLIFGLMA
                     GLVFAVVQRTWLVAIWQALRLHRLRKLDAFLHRFLDSLAGYRANGGLLARVFGTSLLF
                     QMLAIVIIFCISQALNWPVAFIYFCIFVPLITLGEALPISIFGIGVRDSLYVFFFAQA
                     GASREQALSLALVYVLITLVYSLLGGALFLLRRPAAR"
     gene            complement(11377..12063)
                     /locus_tag="PKI64_05615"
     CDS             complement(11377..12063)
                     /locus_tag="PKI64_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE00524.1"
                     /translation="MNLWKGKTMHNKRAFTLIELLVVLAIFAILISILVPVSIKAIEM
                     GNRAKCVNNLKSIGVAFTAYASEHGGALPHHKPLPEGGAGFQETPDFSAIVTKVYTNG
                     YVTDLRVWVCPSDKKDGNSGVSVAKDIGSFRSQQGNCSYMYISGYHLMRTPESLAVAP
                     LLCDEANAREYGPATPGAMPKIGPDDNHGANIRNVLFLDGHVVTFKDADAANAIFDNL
                     RKPELICSVD"
     gene            complement(12060..15032)
                     /locus_tag="PKI64_05620"
     CDS             complement(12060..15032)
                     /locus_tag="PKI64_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00525.1"
                     /translation="MKRLLGIGLLLLLSAAAYATPANTPTLDGRPIEYDAADLRGTFQ
                     GESTWGVNGTLSNLFVTWDATYLYVALQAWQADNNKLVVLLDVDPGAGTGATTTTNWT
                     SVEPSFIKYNDYGWEDASGSFGLDYMLASEGFYNNAIRVNYDGTETPSTNNLESLFDA
                     GNGGTPTGAPVDMASVNDATPCPHKGFEARIPWAVLYEGTRWGTVEAGETVPRGATLH
                     LLAGIHNNNPDSAWSSPDTIPNQTGGDYTNGICATVDYLNVTVDANNDGIPDMLMGDV
                     NAPYIRTAMGAMGGAFIFVVFNETIQAVTAENTANWRVNGAVPSAATVQGPASVMLEL
                     AAPIATNFVVIQASGIEDTANNSRTTEYCLEPAASGIPQAVEVTFQVNTNSGMGISST
                     HARPSAFFLNGSLLPLEWGYPPLETTPLVPLVGSNGWATATVTFPPGSPATLHYKYSA
                     RINGTNNYEAIRLTDFTETSRILDLNTNGTAMTVVDYLGAAAHPLRNHSDTNEPSAQN
                     RLFNDPQRGDAGVRVRREILFQLDLSLRKRDNLARVMVLGSDPLRGFNSTGNNEGGTA
                     SDYPGNSAYLHWDNAGIELFDDGTHGDATAGDGIYSRLWAVSTNGFDAAVEPYAPYSL
                     VGGHEANWLNDIAGTEPYLGEAWWIARRSPRSMIYKFYVLTTGGAHHESPGNNLEYYI
                     ADPDETAPIELPPFLWDNDALPPPPPENAPVFMGVTPSGTTAIVQFENVLTEGGHGIR
                     IATDLAAANPFADYGHRATRISTNGGHAQWSATIEEAKPGREFYAAYAGPEPDPLPVF
                     WEPSFISSAETTWRVHYSQFKGQFKGRRNLSITGLFADWGMGLPMTFLGNGHWVVDVP
                     LPAGSEKVIEYKFRSNGEWMSGDNLKAIRGGGGATWTPDQPTPGELFTVTLDTAGTKL
                     AGATNVNIHLGYDPAWNEASARPMTNTVDSVWEYSVVVPTNYSISVNWVFNGSIVGPL
                     IWYSDADWHAWMTTLVNP"
     gene            complement(15643..16890)
                     /locus_tag="PKI64_05625"
     CDS             complement(15643..16890)
                     /locus_tag="PKI64_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00526.1"
                     /translation="MKSYRLGVSVGLMLLAGWAWAQEPLPSNVAFKLRDVERVVDRAE
                     RVIAVDHIAEADKIAAARSGAAEARGVMAELERRYAGKYDPQHPEIVAMAARIDALEA
                     TAQGREAARTEAQAAAAAQSATASAASADWLARLQPYVIGIGRSGHDPAKYLVPSATH
                     EAEEMQRRLAIHAAASADLEGYRQAHLGANATDELQEVVSTLAVALQDFGNSCAQYAA
                     EDLAEADRQLNEMEAFCRTQEAKVGQGETILFLDRFQMEQAQNVVDRAAGLSKDTAKI
                     DALRARLKAVAQADVRLRATRAADTRLRPDAYGGNDAAELKKAAEDIVAKAAPGCRRL
                     KTIIATPDWRMESVVEWTDSTRTALRHRTTRSVVAECAARVNADTRLYTIHLSQDRQP
                     DGHWGPIQGHVMFTDPMLEANVK"
BASE COUNT         3012 a         4977 c         5884 g         3646 t
ORIGIN      
        1 atccgcgaat ggcgggattt gctgccgcgc tatctgcagt tctggcaaga aggcgggcgt
       61 ggcttggcct tcgttttttt cgaagaatgc gcgcggcgtt ttcccaacga cggcttggcg
      121 ctgaacaaca tggcctggtt gctggcgacg gcggaaccgg acggcttgcg gcatgaacgc
      181 atggcggaat ggccggcgac cgcgctggtt tgggcggagc gggcgttggc gcaagggggc
      241 tccgacatgc ccggtgcttg ggacacgttg gccgccgccc gcgccaacgc cggcgatttc
      301 gccggcgcgg tcgccgcggt cgaacgggcg cgggaattgg ccaaacaggc cggcgaatgg
      361 acgctggacg tccagttgca ggcgcggctc gcggagtacc gcgccggtcg accgtggcgc
      421 gaaccggacg cacgggccaa aaccaccggg agcaagccga ttccatgaag gccgacattc
      481 gcgaaaaact gcgcttggcc ggaatcggac tgctcatcgc ggttgcgctt ttcgccgtct
      541 acgcgccttc gatcggctac gacttcatct tgctggacga tgggccgtac gttcaaaaca
      601 acccgacggt ttccggcggg ttttcctggc cggcggtgaa agccgcatgg accgaagcga
      661 acgaagccta ctgggcgccg ctgctgtgga tgagcttcat gctcgacgtg gaatggttcg
      721 gactgaatcc gcacggattc catctggtga acgtcgtgct cttctccctg aacgccggat
      781 tgctgttcgc gctgttccgg cgctggacgg ggcggacggg cgtggcggtg gcggcgacgt
      841 tgctgtgggc gctgcatccg gcccgcgtcg aaagcgtcgc gtgggtggtc gagcgcaaag
      901 acgtgctgag cggattgttt ttcctgctgg ggttgggcgc ctacgtggag ggacggcaga
      961 gccggctgcc ggcgggcgtc ggcttggcct ggctgtgcat ggcgctcggc ggcgcggtga
     1021 agcagatcct catcgtcatg ccggcggtgc tgatgctgct ggacgtctgg ccgctggcgc
     1081 gcacggattg ggatcggatc ggacgagacg gatggcgtct ggcgcgggaa aaatggacct
     1141 tctggggctt agcggcgttc ttggcgttcc tgccgccttg gttgcatcat caggacgggg
     1201 cgatgatgga cgtgccgatt tcccatcgtc tggcgatgat tcccatccac tatctcttct
     1261 atctcaagaa atccctttgg ccgtccggct tggccgtatt gcagggagat ctgccgttcc
     1321 gttggccggc gttcgcgctg ggaatcgggt tgctggccgg ggcgacgtgg gggctgtggc
     1381 gggttcggaa agcggcaccg tgggcgctgt ggggctgggc gtggttcgtg ggcgccctgt
     1441 tcccgctgag cgggctggtg tgggccgggg cggagcggct ggcgacccgt ttcctctacg
     1501 tgccgcacat ggggctggcg ttggccgtgg ttttggggct tgcccaccgc ggccggaacc
     1561 ggcgggtcat tgccgcggcc gcggcgctgc tggtgttggc cgtctatttg gccgcgacgg
     1621 tccggctgct gccgtactgg cgcgacagcc ggacgctgtt cacgcgcgtg ctgcaattga
     1681 atcccgacag cgtgcacggc ttcgacaatc tcgcccgcgc ctatttcgac gaaggccgct
     1741 ttgcggaatg gcaggcctat ttggaagatt tccgcgcccg tccgtccggg agcggcatcg
     1801 cggacatcca atatgcctgg tggcaggcgg cgatgctcgg cgacgcagaa accgcatggg
     1861 ccaccctcga aaaacgcatg ggcttgccgc gcacggatcc ggatttctgg ctgtggctgg
     1921 agacgcgcac gaacgaccgg atgctgctgg ggacttggcg cgacgtggcc ggcatttgtc
     1981 tccggaccgc aggcgatctg gaacgtctgg agcagatgtg gcagcacggg gaaaaccgat
     2041 gggacgaacg cacccggatg aatttcctgg ccgagatgct gtatgcccat tggtcggccg
     2101 gccgggaggc ggaggcggac gccctggctc gagaagcggg gagtttgtcc ggagcgctgt
     2161 tgcgggcgca gatgccggcg cgcttcgccg agcggtggca acagggcgca cggggctatg
     2221 ccttcgcctg tttccagaat tacgcgcgct gggttccggg cgacgggctg gcgctgaaca
     2281 acatggcttg gctcgtggcg acggcgaaac cggacggctt gcggcacgcg cggatggatg
     2341 aatggcccgc ggcggccgtg gagtgggcgg agcaggcctt ggctgccagc ggcggatcga
     2401 tcccggccgt gtggggcacg ctcgccgcgg cccgcgccaa cgccggcgat ttcgccggcg
     2461 cccaagcggc ggcggaccgg gccctggcgc tggcggaagc cgccggcgag agatccctgg
     2521 cagcgaaatt ccaggcgcag cgggaggaat atcgcgccgg ccggccatgg cgggaatagg
     2581 atgcgcgctg gatttcgcgc gccgaaagcg taatgtcgtc gatatgaagc ggatggtttt
     2641 tttagctttg gcctggagtc tgggcgccac gggtagcggg gccgccgacg tcgtcagcaa
     2701 cgccatgtcc ggcgtgcgct atccgtcgct ggcgaccgcg gtggcggcgg cgacgtccgg
     2761 cgaccggttg gtgatgatcg gcaacgaaac gctcggcgcg acgctgatcg tttccaacaa
     2821 gaccctgacc ctcgtttccg acggaaccgt ccggacggtg accggcagca cgaactgcac
     2881 ggacggaatg gtttcgatcc tgggggtaac ggccacgttg acgctgggcc agccggaagg
     2941 cagcgatgcg gcgccaaccc tggttttcga cggcggccgg taccaaggcg tcaccagcct
     3001 ctccaacatg tttttttcgg atctggggtg gctgaccatc catccgggcg tcgtgctgcg
     3061 caacttggcc agcgtggacg tcggggcgat ctacaatctc agcggcgtgg tggaaatgta
     3121 cggcggccgc atcgaaaaca attccgcgcc ctatggcggc ggcattttca accagacggg
     3181 cgacatctgg atctacggcg gcagcatcac tggcaacgtg gcccagatcg gggggggcat
     3241 ctacaacgac gcggtgcagt ggttcatggg ctatatcgtc ggctattacg gaaaagtgga
     3301 cgtccgcggc ggactcatcg ccaacaacgc ggcgaacgtc gccggcggcg gcatcctgag
     3361 ttacggggcg ctggacctgt cgggcggaca agtgttgcgg aacagcgcgc cgcaaggagg
     3421 gggcgtgctc cacgccaacg gcaaagcccc ttacggcatg tcgttgcggg gtaccgcggt
     3481 cgtcgccagc aactccgcga cgcaaggcag cggcatctac tacaacgacg acggcaacac
     3541 ctggctgacc ctggcggagg gcggacgcgt cgaaccgtcg aacgacgtgt tcctggccat
     3601 cgggaccagt cccgtcattc tggagagcgc gctgacggga cgcggcctcg cggcgcggct
     3661 cacgcccgcc gcctacgcga ccaaccagat cgtgctgggg gcttccacga acagcaacct
     3721 gtggatcgtc agcaactact acggcaagtt taccgtgacg ccggcaccgg gagacccgga
     3781 accttggtat gtgaatgtca atggccgctt gagccgcgat aattcggcgg caacaccggc
     3841 gaccattctg tccatcgcca gcagcgaaac caacgtggaa tggggcgttg agccggcctt
     3901 tcaagcgtgg gatggcgtgg tggagctggc aaccaatctc gtgggacagg cgtggaactt
     3961 ccagccgctg gccccggagg cctattccgt caccaacggg cgcgtggtgg tgccgcgtga
     4021 ccgccccctc ggcatctatc ggctgcgaac gggcggggcc ggcccgtagc cgcgcccgcg
     4081 ggttctcgat cattttaagg tttttgttgg cgggctcgct gagcccgcgg tttgcggtct
     4141 tggattgtcg gctgttcctg taggcgggct cgctgagccc gcggtttgcg ggatcggatt
     4201 gtcggctgtt tctgtaggcg ggctcgttga gcccgcatcc ggggtcaccg cccccggcta
     4261 cagaaggcca taagacaccg gggtcaccgc ccccggctac agtttgaacc ttcgcctcgc
     4321 cggctgtttt tgtaggcggg ctcgttgagc ccgcggtttg cggtcttgga ttgctggctg
     4381 ttcctgtagg cgggctcgtt gagcccgcat ccggggtcac cgcccccggc tacagtttgc
     4441 agaatgttat ttccagaacc aaagctcgcg ccagacttgg gccatatagg ccgcacccca
     4501 gcggaagggt tgtagtttgc gttcccctcc gatacgcggg ggttcatctt cggggatttc
     4561 gccaatcttg agcttgcgtt tcgccgcgcg gatggaaagc aatggttcaa tgcccaagac
     4621 cgtgccgaac catttttcgg gggtgtagct ctcttccttg tggagatcca actcgtaaaa
     4681 aagttgtgtg cgataagccc tgtaaatcac catggcatcg gtgtagggcc ggcgccagga
     4741 gtggccgtgg agcaaattga tgctgtgcgt gaacagccag ttgccgaagg cggtcatggg
     4801 gtcgtcgtcc tcgcttttgg ccggggggag atagcgtgag gcgatgacca tgtcataccc
     4861 ctttttcatt tcttcaatga gcagggggat ggagcgcacc ggcgagttgc catcggggct
     4921 gaaggtgatg accacatcgc cccgcacatg cgggaaagct tcaatataag cccagcggat
     4981 gcccttgccc ttttgttcaa tgacatcgta cccctgtgat cgggcgtagt cggcggtgcc
     5041 atcggtggaa tagtcggcca ccaaaatctg atccacccag gaacggtcaa tttgcggcat
     5101 gatgacctgc atggcttcga tttcattgcg ggtggggatg aagagcgtgg ttttcatggg
     5161 gtgcggctgc ctgtggttag ggtgaaggac tgtatcatga tgttggcgtg gtggcgagga
     5221 gttcggcggc gtgacggagg gtgacgctgg tggattcgcg accgccgagc attcgggcga
     5281 gttcttcgac ccgcgcggcg tgatcgagcg caacgacgcg cgtatgggtg cggccggctt
     5341 ggacttcctt gcgcacagcg tgatggtgcg cgccgcaggc tgccaccgtg ggcagatgtg
     5401 tgatggcgat gacttgccgc cgtttcgcgg cttgggccag tttttgtccg acggcgcgcg
     5461 cggtttcgcc gccgacgttg gcatcaattt catcaaacac cagcaggggg atgcgatcgg
     5521 cggcggccac caccaccttg atgccgagca tcacccgcga tatttcgccg gaggaggcaa
     5581 tggcgcgcaa cggccgcatg ggttcgccga cattgggggc aaaggcaaat tccagtttat
     5641 cgaggccgga agccaacggg gcctcgctgg gggttagctc gatggcgaaa aggccatggg
     5701 cgaagcccag ggcctgtaac tcagccgtga tggcgggggc cagtttttcg gcggcggcgg
     5761 cgcggcgttt acgcagggcg cggcccgcgg tttgcagctc tttttccacg gccagcagtt
     5821 ctttttcaac atccgctttt tgctggtcgc gcgtctgcaa atcttgcagc cgggttttgg
     5881 cggcagcaag ggtttgaagg atatcagcca agctggggcc atatttgcgt ttgagcttgt
     5941 cgtaggtggc aaggcgctgg tcgagccatt ccagtcgcgc gggatcggcg gagatgtggg
     6001 taagttcgct ctggatggcg cccgtgagcg cggcgagggc atcggtgtgg ccgcgaaact
     6061 cggccagcca ttccttggcc gcgggcagca agcgggcaag ctcatccagc gctttgcggg
     6121 ccggcgccat gatggccagc gccgagtttt cgtcttcact ggtggcctgc acaataagct
     6181 ggcccaactc caggatgcgc tgggcgtggc cggcggtttg ctgttcggcg cggacttcgt
     6241 cttcttcgcc ttccacgggg gcggcctctt cgatttcccg cacttggtgg gcgtagagcg
     6301 cgatttgcgc atccagatcg ccgcggtcgc cggcgagttc ctcgcggcgg gccagcaacg
     6361 cttgacgccg cgagaaagcg ttggcgtagg cggcgcgttc ggcatccgcc cgcgcgaagc
     6421 tgtcgaggat gtccagttgg ctggccggct ggaagagcga ctggtgatcg tgcgggccgt
     6481 gcaagtccac caagtgggcg cccagacgct tgagcagcgg cagcgtgacg ggggcatcat
     6541 tcacgaaagc ctgcccgccg cccgcggcct tgaccgtgcg gcgcagaatg agcaggccgt
     6601 ggtcgcatgg ctccaagccg gtgtcggtga ggatatcgtt gaccacttgc ggttcggcga
     6661 gttgaaacgt ggcctccacg acgcattggg tttcgccggc gcggatggcg gatgtatcgg
     6721 cacgttcgcc cagcagcaga tatagcgcgc cgatcaggat ggatttgccg gcgccggttt
     6781 cgccggtgat gatgttcagc cccggctcaa aggccacggt ggcttcttca accagggcga
     6841 ggtttttcac tctcaggagt gtcaacatgc gggcagatta ccgcaggcgc ggattcggca
     6901 aaagagggaa gttgcgcgga aaaacgggga gcttaccaag aatcgaaagc ggccgcgccg
     6961 cgggaggtga tttggccccg gccgtccgcc gcaatggttt ggcccgcatc atctacgata
     7021 atcagcgtgg gaatcccgtt gacgcggaat ttggattgga gtgcgttgcc tactttgtcg
     7081 ccaaacggaa tcgcgagcca aggcatatta aactcgtcca tatagcggcg catgtcggcc
     7141 tcgctgcggt ccaggcagat cagcacaacc tcaaacggct tgctggccgt ctgcatttcg
     7201 ttgtaggcct tgacgagcac gggggtgaag gcgtggcagg gcgggcacca ggaggcggag
     7261 aaatagaggc cgattttttt gccggccagc gtggccggcg aaacacggtg gcccttggca
     7321 ttgacgagct tgcgacccac caagtcttgc agggccggtg ggatgggggc gtcttcggca
     7381 tcggcggttg atttccggtt cagacccgca gtgcgacgtt cgccgagaaa ccgttggtcc
     7441 gcggggctaa gctgattgag gcggatgcgg agtttttcgc catcgggctt ttgtaggatg
     7501 accacatcgg cctgctggct tacaaacgcg gcttcgatgg tggctccgct ggcgctggtc
     7561 catgtccgga tgtcttgcgc ccgcgcaccg gttatggcca ccatcaataa gagcaaccca
     7621 aagaatacat gtttcatacc tgtctcccag ttgggcccaa atcctgggcg aaaccaccgg
     7681 ttgaagcgag cagaaaacag ttggtgatcg tgcttccctg ctggcggtat tttccgtaaa
     7741 gtatcggggc aaaactcaat ttcgaggaat tactgtaatg agtggcattt gcgggttcac
     7801 gtggcgggat ttcaacttgg ggcagcggat ggcgtccgtg atggcctatc gcgggccgga
     7861 ccatgagggc gtgtggtgcg atgaccacat gacccttgcc caccggcgac tggatgtgat
     7921 ggacctttct gccgccggca accagccggt ggccagcgcg gatggccaga aacacctggt
     7981 ttttgatggt gaaatctaca acctggccgc ggtgcgccaa gagctggcgg ccaaggggcg
     8041 ggtgccggcc gaggccgcaa gcgccgtggt gttgctgggt gcctacgagg agtggggccc
     8101 ggcgatggtg cacaagctgg tgggctcgtt cgcaattgcg ctgtgggatg cggcgcggcg
     8161 gcggctttgg ctggtgcgcg accgactggg catcaagccg ctgtattact atttcaagga
     8221 cgcccggctg gtgttcggct ccgagatcaa gtccattctg gaggatgcgc agattccgcg
     8281 gcgggtgaac catgccgcgt tgtatgccta tctgggtttt gagttcattc cggcgccgga
     8341 taccgcattt gagaatatcc gcaagctgcc ggccggccat tgggccacac tggaaaatgg
     8401 ccagttgcgc gtggaggaat attgggattt gcgcatggag acgccagcca cgcccctcgg
     8461 cgagggcgaa gtagtggagc gtatccgcgc gctgattgat gaggccgtac gcgagcaact
     8521 cgtttcctcg cgaccgctgg gcgcgttttt gtcgggcggc ttggactcca gcacgctggt
     8581 ggccatgatg cacaagcaca gcgcgggcaa ggtcaggacg ttcaccattg gcttccctga
     8641 caaaaccttc agcgagctga attatgccca gaccgtggcc gaccatttcg gcactgagca
     8701 tcaagtgttg atcattgacg acatggatga ggccgcgctg gagaagtcca tctggcatct
     8761 ggatgaaccg atgaccgatc tttccgccgt gccgctgatg tttttgtgcg agaaggccaa
     8821 ggcgcatatt tccgtttgcc tgagcggcga ggggggcgac gaagtttttg tgggctacga
     8881 ccgctataaa gccgccaaga tcgcccgttt cctgaatgtg ctgccgcgct gggtgcggca
     8941 taacctgatc ggcaaatgga cggccagtct caaggaccgg ccggagaaaa aagggccggt
     9001 caacatgttc aagcggttca tggagggcgc gctgctgcct gaagatggcg agcacgtgcg
     9061 ctggcagtat ttcatgaacg cgcggtttga gcagcagctc ttcaccgcgg gctacaaggc
     9121 gcaagtggcg tttgatccgt ttgcgcgggt gcgggcgcac agcgccaagt gcgccacccg
     9181 cgacaaggtg aacctgcatg cctatctgga cacgcgcttc atgatgcccg atggcgtgtt
     9241 gatgaaggcc gacaagctga gcctggcgtc ctcgctgctg ctacgcgtgc cgctgctgga
     9301 ccaccggctg gtggaataca tgggcacggt gccgggacac ttcaagctgc gcggcctgac
     9361 caccaagtac attttccgca aggcgctgga aggcatcctg cccaaggaaa tcatctggcg
     9421 cggcaagcag ggctacagcc tgcccgtgaa gaacctattg cgcaatcagt tcaaggacta
     9481 catgaccacg ctgctgcatg agtcgccgct gattcgcgag gactttgagt tggcttttgt
     9541 ggaccagttg attgccgagc acttgaacat gcagcacaac cataaccatg tgctgtgggg
     9601 cctgatgaat atcgccatct ggcaccgccg ctatttcccg catggttgat tgggacggac
     9661 gatgaccaca ggcgcggaat acaaggttaa ggaagtcacg cagggttccg ccgcgggcgc
     9721 ataccgggcg ctgatgtatg gcgacatgag cctcggcaag gtgatctggg cggaaatcct
     9781 cttcacgctc ttgggggggc ttggcggtgc ggtggggctg tttttgcgga tgaagctcta
     9841 ccccacgctg ttcaaatcgt gcgggcgcaa ggtggtgttt ggccggaatg tgacgatccg
     9901 ccatccgcac aaaattgtgc tgggcgatgg cgtggtactg gacgacaact gtctcgtgga
     9961 tgccaaaggc gccggcaaca ctgggatcat tctgcatgag cgggtgtatg tgggccgcaa
    10021 cacacttatc tattgcaaga atggcgacat tgagatcggc gccaatgtca gtctgtcggc
    10081 caattgcgtc atcttttcgg ccaatcaaat cacactgcaa cccaacagcg tggtggcggc
    10141 gttcgtctat ctgttgagcg gcggtgaata tgatttggcc gacccgacgc cattcgcttt
    10201 ccagaaagga acctgcacca aggggccgct gaccatcggc gcggattgct ggatcggcgc
    10261 gggcgccaag gtgctggatg gcgccagctt gggcgaccgc gtggtgctgg gcgcgggcgc
    10321 cgtggccacc aagccggtgc cggcgcattc catcgccgtg ggcgtgcccg cccgcgtcat
    10381 caaaacccgg caggtgcccg gttgaaaaaa cgactgacgt tgctgggcaa attgctggtg
    10441 atgagcggcc tgctggcgct gctctatcgc aaggcggact gggcgagctt ttggggggcg
    10501 ctcacggatt tgcaatgggg ctggttgccg ttgatttacg cgctgctgct gttcaacacc
    10561 gccatcagcg cgtggaaatg gcaacaactg ctgcgcgcgg atgacgtgcg ggtgccgttg
    10621 cggtcgttgc tggtcagtta tctgatcggc accttcttca atctgttttt gccgtccagc
    10681 attggcggcg attcctatcg cgtggtggat gccagccggc acggcggcgc ggccaaaagt
    10741 tttgcgtcgg tcttcgccga ccggctgact ggttttctcg cgctggccat ttgggggttg
    10801 gtgttttcgg ccatcgggtg gtcgcggctg ccggataaac ggattctgtg gctgccggtg
    10861 ttgattttcg ggctgatggc cgggctggtg tttgcggtgg tgcagcgcac gtggctggtc
    10921 gccatttggc aggcgttgcg gttgcaccgg ctgcgcaagc tggacgcctt cttgcaccgg
    10981 tttttggatt cgctggcggg ctaccgcgcc aacggcggct tgttggcgcg ggttttcggc
    11041 acctcgctgc tgttccagat gctggccatt gtgattattt tctgcatctc gcaagcgttg
    11101 aactggcccg tggcgttcat ctacttttgt attttcgtgc cgctgatcac gctgggcgaa
    11161 gccttgccca tttccatttt tggcattggc gtgcgcgaca gcctctacgt gttctttttt
    11221 gcgcaggcgg gcgcgagccg ggaacaggcg ctctcgctgg cgctggtcta tgtgctgatt
    11281 acgctggtct attcactgct gggcggcgcg ctgtttctgc tgcgccgccc ggcggcccgc
    11341 tgacgcagcc cggcttcgtg gcgcacaggg gcgggatcag tccacgctgc aaatcagttc
    11401 cggcttccgc aggttgtcaa agatggcgtt ggcggcatcg gcatccttga acgtcacgac
    11461 gtggccatcc agaaacagta cgttgcggat gttggcgccg tggttgtcgt cggggccgat
    11521 tttgggcatc gcgccgggag tggcgggacc gtattcgcga gcgttggcct catcgcaaag
    11581 caggggcgcc acggcaagtg attccggggt gcgcatcaga tggtagccgg agatatacat
    11641 atagctgcaa ttgccctgct gtgaacggaa cgagccgata tccttggcca ccgagacgcc
    11701 ggaattgcca tcctttttat cgctggggca gacccaaacg cgcaagtcgg tgacatagcc
    11761 attggtgtac accttggtga caatggcgga aaagtccggc gtttcctgga aaccagcgcc
    11821 accttccggc agcggcttgt ggtggggcag cgcgccgcca tgttccgacg cataggcggt
    11881 gaacgccaca ccgatgcttt tgaggttgtt tacgcatttg gcgcggttgc ccatctcgat
    11941 agccttgatc gaaaccggta ccagaatcga aatcaggatg gcaaagatgg ccaaaaccac
    12001 cagcagttca atcaaagtga aggcgcgttt gttgtgcatg gtctttcctt tccaaagatt
    12061 catgggttca ccagggttgt catccaggcg tgccaatcag catcgctata ccaaattaac
    12121 ggaccgacta tcgatccatt gaaaacccaa ttgacgctga tggaatagtt cgtgggcacc
    12181 acaacggagt attcccagac gctgtcgacg gtattggtca tgggccgcgc gctggcctcg
    12241 ttccaagctg ggtcatagcc gaggtggata ttcacgttgg tcgcccctgc caacttggtg
    12301 ccggcggtat ccagcgtgac agtgaacagc tcgccggggg tgggttggtc gggggtccaa
    12361 gtggcaccgc cgccgccgcg aatggccttt aggttatcgc cgctcatcca ctcaccgttg
    12421 ctgcggaact tgtattcgat gactttctcg ctgccggcag gcaacggaac atccaccacc
    12481 caatggccgt tgccgaggaa cgtcatgggc agacccatcc cccagtcggc gaaaagcccg
    12541 gtaatgctca agttgcgtcg gcctttgaat tgtcccttga attggctgta gtgcactcgc
    12601 catgtggtct ccgcggagga aatgaaactg ggctcccaga aaactggcag cgggtccggt
    12661 tccggcccgg cgtaggcggc gtagaactcg cggccaggct tggcttcctc aatggtggcc
    12721 gaccactggg catggcctcc gttggtagaa atgcgcgtgg cgcgatggcc gtagtcggcg
    12781 aacgggttgg ctgccgctag atcggtggcg atgcgaatcc cgtggccacc ctcggtcagg
    12841 acgttttcaa attggacgat ggcggttgtg ccactcggtg tgacccccat gaaaaccggt
    12901 gcattttcgg ggggtggggg gggcagagca tcgttgtccc agaggaaagg cgggagttcg
    12961 atgggggcag tttcgtcggg gtccgcaatg taatattcca gattattgcc ggggctttcg
    13021 tggtgcgcgc caccggtagt gagcacatag aacttataaa tcatgctgcg cggggagcgc
    13081 cgggcaatcc accatgcttc acccaagtag ggctcggtgc cggcgatgtc gttcagccaa
    13141 ttggcttcgt gtccgcccac gagggagtac ggcgcgtagg gctccacggc tgcgtcgaac
    13201 ccgttggtgg aaacagccca gaggcgcgag tagatgccgt cgccggccgt ggcgtcgccg
    13261 tgggtgccat cgtcaaagag ttcaatgccg gcgttgtccc agtgcaggta ggcgctattg
    13321 ccgggataat cgctggcggt gccgccctcg ttgttgcccg tcgaattaaa gcctctgagc
    13381 gggtcagacc cgagaaccat gacgcgggca aggttgtcgc gcttgcgcaa cgagaggtcg
    13441 agttggaaca gaatttcgcg gcgcactcgc acgccagcat cgccacgctg cggatcgttg
    13501 aacaagcggt tctgcgcgga aggttcgttg gtgtcgctgt ggttgcgcag cgggtgggcc
    13561 gccgcgccaa ggtagtccac cacagtcatg gccgtgccgt tggtgttcaa gtccaatatg
    13621 cgcgaagttt cggtgaagtc ggtcaggcgg atggcttcgt agttgttggt gccgttgatg
    13681 cgtgcgctgt acttgtagtg gagcgttgcc ggcgagcccg gcggaaaggt gaccgtagcc
    13741 gtggcccagc cgttgctgcc gaccaacggc acgagcggcg tggtttccag cgggggatag
    13801 ccccactcca acggcaacag agaaccattg aggaagaaag ccgagggccg ggcatgggtg
    13861 ctactgatgc ccatgcccga gttggtgttg acttggaatg tcacttcgac ggcctgcggg
    13921 atgccgctgg cggccggttc gaggcaatac tccgtggtgc gcgagttgtt ggcggtatcc
    13981 tcaatgccgc tggcctggat caccacaaag ttggtggcga tgggcgcagc caactccagc
    14041 ataacgctcg ccgggccttg caccgtggcg gcgctgggca cagcgccatt cacccgccag
    14101 ttggcggtgt tttcggcggt gacggcttgg atggtttcgt tgaaaaccac aaagataaac
    14161 gcgccgccca tggcgcccat ggcggtgcgg atatagggcg cgttgacatc gcccatgagc
    14221 atgtcgggaa tgccgtcgtt gttggcgtcc accgtgacat tcagataatc caccgtggca
    14281 cagatgccgt tggtgtagtc gccgccggtt tggttgggga tggtgtccgg cgaactccag
    14341 gcactgtcgg gattgttgtt atgaatgccc gccagcaggt gcagcgttgc gccgcgcggc
    14401 acagtttcgc cagcttccac ggtgccccag cgagtgcctt cgtagagcac ggcccaaggg
    14461 atgcgcgcct caaaaccctt atgcgggcaa ggggtggcat cgttcacgct ggccatgtcc
    14521 accggcgcac cggtgggggt gccgccgttg ccggcatcga acagcgattc cagattgttg
    14581 gtggagggtg tctcggtgcc gtcataattt acgcgaatgg cgttgttgta aaacccctcg
    14641 ctggcgagca tgtaatcgag gccgaagcta ccggaagcat cttcccagcc gtagtcatta
    14701 tatttgatga agctgggttc tacgctggtc caattggtgg tggtggtggc gccggtgccg
    14761 gcaccgggat ccacatccag caggacaacc agcttgttgt tgtcggcttg ccacgcctgc
    14821 aaggccacat aaagataggt ggcatcccac gtgacgaaca ggttggagag cgtaccgttg
    14881 actccccaag tggattcgcc ttggaatgtg ccacggaggt cggcggcatc gtattcaatg
    14941 ggtcgcccgt ccaaggtggg ggtgtttgcg ggtgtggcat acgccgcggc ggagagcaga
    15001 agcagaagtc cgatacccaa caatcttttc acagcctacc tccagtgtgt gtcggtacta
    15061 tatccgatgc aggacagcaa aaaaagaaaa acaaatctgt ttcgagggtt tggggtttag
    15121 gggcagtggt aagggcttca gaattctggc aggcggttcg ccataccttg aggccgttcc
    15181 ttacttgccc tcctgttgac ccgtccttgt cggtcggagt tgtgctgtgc acagtggaca
    15241 aggcccggcc cttctgtagc cggcctcagt gaagccggtt tccggaaaga ttgtggaggt
    15301 tgtactccgg cggctgtttt tatagaggaa tgcctccgtg ttgtcctgtt ggttcggcgg
    15361 tcttcacctg acctttgttc tttcctgcat cctgtccctt ccgtcgtgtt gtggcggtgc
    15421 gttatcatca aaaaatgcac cggttagtgt ggcgcagcgg tccattcatg tactatagtg
    15481 aattcacata ttgcaataag tgaattcaca aattgcaata tgtgaaccat ggtgcagacg
    15541 atggataatc ctgctgtttt gccccagaat aaacgctaca tagggccaat tctcgtatta
    15601 caatgattag cgaggattgg ctcgggcgca gggggtggac cgctacttga cgttagcttc
    15661 gagcatgggg tcggtaaaca tcacatggcc ttggatgggg ccccagtgtc catcaggctg
    15721 acgatcctgg ctgagatgga tcgtgtatag ccgggtgtcg gcatttaccc gcgcggcaca
    15781 ttcggccacc acagagcgcg tcgtgcggtg gcgcagcgcc gtgcgcgtgg agtcagtcca
    15841 ctccacgacg ctctccattc gccagtcggg tgtggcgata atggtcttga gccgacggca
    15901 gccgggcgcc gctttggcga cgatgtcttc tgctgctttc tttaattctg cggcatcatt
    15961 tccgccgtag gcatcgggcc gcagacgcgt atcggcggcg cgggtcgccc gcaggcgtac
    16021 atcggcttgc gccaccgcct tcagacgggc acggagcgca tcaatcttgg cggtatcctt
    16081 gctcagtcct gccgcacggt ccaccacatt ctgcgcttgt tccatctgga aacggtcgag
    16141 aaacaggatt gtctcgcctt ggcccacttt ggcctcttgt gtccgacaaa aggcctccat
    16201 ctcgttgagt tgtcggtccg cttcggcaag gtcttccgcg gcatattggg cgcaggagtt
    16261 gccaaagtct tgcaaggcca ccgccagggt actgaccacc tcttgcagtt cgtcggtggc
    16321 gttcgcgccc agatgagctt ggcggtagcc ttccaaatcc gcgctggcgg cggcatggat
    16381 cgccagccgc cgttgcattt cctctgcctc gtgggtggcg ctggggacca gatacttggc
    16441 aggatcatga ccgctgcgac cgatcccgat cacatacggc tgcaggcgcg caagccaatc
    16501 agcggaggcc gcgctggccg tggcggactg tgcggcggct gccgcttgag cttccgtccg
    16561 ggccgcctcc cggccttggg cagtcgcttc cagcgcgtca atgcgcgctg ccatcgccac
    16621 gatctccggg tgctgcggat catacttgcc cgcatagcgg cgctccagtt cggccatgac
    16681 gccgcgcgcc tccgccgccc ccgaccgcgc ggcggcgatt ttgtccgcct ccgcgatatg
    16741 gtccacggcg atgacccgtt ccgcgcggtc cacgacgcgt tcaacatccc gcaacttgaa
    16801 tgccacattg ctgggcaacg gttcctgcgc ccatgcccag ccggcaagca acatcagccc
    16861 cacactcaca cccaaccgat acgatttcat tcgtactcct ctttcctgat atgtgtcgtt
    16921 cgccgtgacc gttccgatca tcatacgagg gccaaccgga atccacaccg cccacagcag
    16981 tgatgccaag gtaatgattt tttcctgccg tgaaaatctt taattctcca tccgcgtttt
    17041 gggagaggcg gtctccgtgc cgtcgtctgg gctatggctg atgtttttct tgtagccggc
    17101 ctcggtgggg gccggggctg ttggctcgtg tgctttgctt cttccgacgc ttgggatgga
    17161 ggattttttg cagaatttgg cggcgccaaa accaaccggc atcttttccg cgctggcgcg
    17221 tcgcgcccga ccgcttgcgg acacgagtcc agctcgggtc gtccgcaacg cgccatcatc
    17281 ggaaaatcta tcggttattt tggcgcgaca gccccgccaa aatccgcaag aaatcctcca
    17341 catgcacgtg ctgcgcacag cgaacaagag cgggttcgtg actcctcctg ccgtgcgcgc
    17401 ggggcgcgga ctgttgtggg acaggattcc acaccgtgga aaatcccgcc aacgcgggat
    17461 aagtattttt ccacactctt atgagaaggc atgtcaagag gtccgttttg acggacacc
//
LOCUS       DAOPNP010000045        17485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3875_length_17485_cov_7.772002, whole genome shotgun sequence.
ACCESSION   DAOPNP010000045 DAOPNP010000000
VERSION     DAOPNP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_17485_cov_7.772002"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..643)
                     /locus_tag="PKI64_05630"
     CDS             complement(92..643)
                     /locus_tag="PKI64_05630"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOE00527.1"
                     /translation="MHLLFPFLMVVVARIFDVSMGTMRVAFIARGRKVFAACCGFIEV
                     FIWICVVSQVLSGERHLAIYVAYATGYACGTLVGMFIEEHLAVGLVLVQVITTRPAEP
                     LMQQLSAAGYGVTRQDAQGTRGPVQVLSILASRRRVNDLRAIVHAFDPDAFYTISDVR
                     QARDIPAAYATAGTGAGKSRMPV"
     gene            complement(666..1466)
                     /locus_tag="PKI64_05635"
     CDS             complement(666..1466)
                     /locus_tag="PKI64_05635"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOE00528.1"
                     /translation="MTATLPADTHTHTALCRHAGGVPLDYARAAAARGIKEIACTEHI
                     PFPNDPAPAIRMTADEFPRYLELVREAQQAGLCEVLLGIEADYQRDLIGEHIPRLLAA
                     ADFDLVLGSIHTGPFWDLGPNDPNATPEFVAQMNRTYYQRMKEMAQTGLYDICAHFDL
                     VKRAGIHAPTALLAEIVPPALDAVAATGMAIEINTSGFRHRAGEAYPSLTILGWMKER
                     DIPIVFGSDAHNPDDVGQHFEPAVALARAAGYTHYAAFRKRRFTLRPF"
     gene            complement(1502..1795)
                     /locus_tag="PKI64_05640"
     CDS             complement(1502..1795)
                     /locus_tag="PKI64_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00529.1"
                     /translation="MMPAYQLHEMLIRIGLALGAGVLIAITPIAYRRTRFEPSRIKRV
                     IFYVIGLAALVGFGFYARYLWNTFVPHEPETPAAITYPAAPGAPVSPVRPVAR"
     gene            complement(1835..3502)
                     /locus_tag="PKI64_05645"
     CDS             complement(1835..3502)
                     /locus_tag="PKI64_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOE00530.1"
                     /translation="MKKNITITLESGQKITCPSQTLVGELLHEPNDPVSGLPYLGALV
                     NNELVTFAYTLDIDATVRFVTMATPGGMQIYIRSLSFVLAKAVKDLHPEAQFSVEHAL
                     GPGLYCEFEVNGQRGMTPAQAEALEAHIRDLVTTNRPILRQRISYAAAIEQFTRNGQM
                     DKVSLLRFTNTPKIVTYVCENFSDMSTGPMARSCGPLRYFKIIPYQTGFVLQMPTVDT
                     APNVPEFKPVTVLAETFKEYNRWGRILNVNTVGRLNEIIAADEIHDFVKVAEALHEKK
                     IAAIADQIAATPTVKWILIAGPSSSGKTTFAKRLAIQLRVNGLRPVTLGTDDYFVDRE
                     HTPRTPTGEYDFEHLQAVDVETLNRDLADLDAGREIKLPTFDFQSGARTYKGRTMRLA
                     EDQVVVLEGIHSLNPALTPAIPEGRKFHIYISALTQLNLDNNNRIPTTDNRLLRRLVR
                     DHRYRGHSATRTLEMWPNVGRGERTWIFPFQEEADAVFNSALDYELAILSVLARPLLS
                     EIKPNQPIYAEARRMLEFLSHFLPLAADVPPPTSIIREFVGGSAFEY"
     gene            complement(3606..4190)
                     /locus_tag="PKI64_05650"
     CDS             complement(3606..4190)
                     /locus_tag="PKI64_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00531.1"
                     /translation="MDALFHLRDMIASLDGPLVAALAARARLLRNDAIYAVNQTPPAG
                     LDALATSYAKSRTLAGRVQLLRAPYLSVIVPRLCVPGDAPLEREACLAADIACLNAMA
                     RRLALSVHVATRKRQALPQTLQQAVATRNPDVVEQAITNAAVEADVLARVAAHARKAE
                     ATPGTIDAITQFYADWLIPLSRKIQVHDLLSPAE"
     gene            complement(4195..5034)
                     /locus_tag="PKI64_05655"
     CDS             complement(4195..5034)
                     /locus_tag="PKI64_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOE00532.1"
                     /translation="MNTPILYLGPEGTHSHEAAIRRFGQKAKLLPCLSHHEIFSRLVT
                     ARGANRPQAVVPVENSSEGPVTQTLDQLANHPTVGIMDSFSLPVPQHLMARRGTALAD
                     IRRVCSHPQVLGQCRENLARLLPRAELVPESSTAVAAGRAAQEPGTAALASAAAARIQ
                     RLTILQKNLQDSPENTTRFFTLTTGKVPQAAGKSTRLRSLIYLVIGNRPGALLHVLAP
                     FDAAEINLTFIQSRPLPGRPWEYGFFIEAATDWSSDSAQAAWQLVRALSESGRKIGTY
                     PAK"
     gene            complement(5052..6086)
                     /gene="aroF"
                     /locus_tag="PKI64_05660"
     CDS             complement(5052..6086)
                     /gene="aroF"
                     /locus_tag="PKI64_05660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE00533.1"
                     /translation="MIIVLKRNCSETDIATVSDMVCTLRYQPRIIRGVEQTVVACIGD
                     ELSHQSLEVLRNLPAVESVFPVQKKFKLVSREYHPGDSVVTVKGVALGGGNIEVIAGP
                     CAIESYDQFRTAVKDLTACGIRIIRAMPFKPRTSPYDFQGLKQDGLEIMREVKQEFDV
                     AMISEVPGPDKIEPLLEVADMFQIGARNAQNYDLLEHIAQAGRPVLLKRGLASTVEEW
                     LTAAEYLIVNNCTEVALCERGLRSFDSSTRNLLDLGAVAVAKQLSHLPVIVDPSHAAG
                     ARRLVPALARAGLAVGADGLIVEAHPNPLSAFSDAPQQLETANFQKFMDDLAPWLELA
                     RQQRTRPTPS"
     gene            6476..7783
                     /locus_tag="PKI64_05665"
     CDS             6476..7783
                     /locus_tag="PKI64_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE00534.1"
                     /translation="MTTATDLLAKIKSRRARLAVIGLGYVGLPLAAEFGKHYDVVGFD
                     INPQRLQELRAGNDRTLEVSTADLRLATRLTYSDKIEDLRGTDVFIVTVPTPIDRHRR
                     PDLTPLVKASETVGRALQPGGVVVYESTVYPGCTEEDCVPILEKFSSLKFNVDFFCGY
                     SPERINPGDKVHTVTKIKKITAGSTPEIGRAVNALYASIITAGTHLAPSIRVAEAAKV
                     IENSQRDINIAFVNELALIFARMGLDTHDVLTAASTKWNFLPFRPGLVGGHCIGVDPY
                     YLTHKAESLGYHPEIILAGRRLNDNMGAHVANRVVKLLIQKGRRVHGANVLVLGVTFK
                     ENCPDIRNSHVVDVVRELQDFGCRVVVHDPWADAEEVRHEYGFELLPALAKPAFYEGF
                     DAVILAVAHEAFRDLTFPPADQRKLVVFDIKSFLARDRVDGRL"
     gene            7899..9212
                     /locus_tag="PKI64_05670"
     CDS             7899..9212
                     /locus_tag="PKI64_05670"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOE00535.1"
                     /translation="MPKMKEIGVAVLGFGTVGAGVVETLQKNGHLLADRLGIRLVLRG
                     VADLDLTTDRGVKVDPALLTTDGAALIARPDVQIVVELIGGTGVAKQFILQALQLGKT
                     VVTANKKLLAEHGEEIQRVADEHQAEILFEASVGGGIPIIKALREGLCANRIERIYGI
                     LNGTCNYILTRMEEEKLPFAEVLKAAQAAGYAEAEPSLDIDGHDTAHKAAVLASLAYG
                     GHVPLAKIRVTGIRHLAAADIAYAAEMGYRIKLLAIIQGDANGIEVSVQPTLVAHSHQ
                     LAAVGGVFNAVLVQGDVVGTTLYYGRGAGRAATASAVVADLADAALNLQAGGRRRDLS
                     AVQSRAPVAFRDPGEIVARNYLRFSMRDKPGTLASVASILGRHQIGIDSVMQHEAPSP
                     ADYVPVIIVTGPAPEREMDAALAEIAATSGIVNQPAVRYRIEDFD"
     gene            9244..10506
                     /locus_tag="PKI64_05675"
     CDS             9244..10506
                     /locus_tag="PKI64_05675"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOE00536.1"
                     /translation="MALYVQKYGGSSVADAECMRRVAQRVFDTQKAGNQVVVVVSAMG
                     DTTDDLIALAKQVNPEPDEREMDSLMATGEMASSAILTMALHSLGAKAISMTGQQAGI
                     QVDGTHLKAKIEKINPSRIRQHLKQGYIVVVAGFQGMNPDADVATLGRGGSDTTAVAL
                     AAVLKADRCQILKDVDGVFTANPRVVPTARKLDEIAYEEMLELASCGAEVLQSRAVEF
                     AKNYNVVLEVMSSFEVKPGTLVREENKQMEQMIIRGIAADKNQAKVTLLSVPDTPGVA
                     AKIFKALGNAKVNVDMIVQNVSAAGVTDISFTVAKDDLNKTKRVVEPLVRDIRATGAN
                     FSDDLAKVSLVGVGMKSHSGVAFKMFDVLAANKINIEMISTSEIKLSVVILAKDTDRA
                     VRALHEAFGMGVRARQAKAAPKKKTARR"
     gene            10543..12138
                     /gene="cimA"
                     /locus_tag="PKI64_05680"
     CDS             10543..12138
                     /gene="cimA"
                     /locus_tag="PKI64_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOE00537.1"
                     /translation="MKKVSIYDTTLRDGAQGEGINFSLPAKVRIARALDEFRIDYIEG
                     GFAASNPKDMAFFEEMKKVKLRHAKLAAFGSTRRASKTAEQDPGLAALLQAGTPVTTI
                     FGKSWLLHVEKVLKTTAAENLKMIADSVAYLKSHGKEVIYDAEHFFDGYLDNADYALQ
                     TLQAAADAGADALVLCDTNGGRLTSEVVSITQVVAARFPKARIGIHTHNDAELAVANS
                     LSAVESGATMVQGTMNGYGERTGNANLCSIIPGLALKLGAKLSCGARLQKLRSISVLV
                     DEIANVRPLKSRPYVGQSAFAHKAGMHVDAVSKVSRSFEHIEPESVGNERRILMSELS
                     GASNVRLKTGEMGLEFQKDAPAIKDILHKLEHLEKNGYAFESADASFKLLVQKVLKKH
                     VPFFELQGFSVVVQKRDADSKATTTATIKISVKGKTELTAGEGDGPVDALNNALRKVL
                     HQFYPQLDSVVLQDYRVRILNPETATQATTRVLIESSDGRERWGTVGVSENIIEASWE
                     ALVDSMEYKLFLDEQRRQKRQRK"
     gene            12253..14940
                     /locus_tag="PKI64_05685"
     CDS             12253..14940
                     /locus_tag="PKI64_05685"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOE00538.1"
                     /translation="MSELPKTYDPQAIEAKWYDWWKQRGFFRAEAEWGGQPYTIVIPP
                     PNVTGILHMGHALNETIQDVMIRRKRMQGFNTLWVPGTDHAGIATQNVVERQLKEQGK
                     SRWDMSREEFLKLVWAWKEQYGGTILRQLEKLGNSCDWSRTRFTMDEGLSEAVAETFV
                     RLYDKKLIYRGEYIINWCPRCETALSDEESEHTEQAGRLYHIRYPVKGGRKSEYVVVA
                     TTRPETMLGDTAVAVNPRDERYAKFKGKTLVLPILNREIPVIEDDFVDPKFGTGAVKV
                     TPAHDPNDFDMGRRHALPMINVMTPRAQMNENAGPYQGMSREECRKKLVADLQAAGLL
                     VKIDEHAHAVGHCYRCHTAVEPRLSPQWFVKMKPLARPAIDAVKDGRIRFVPERWSKV
                     YLEWMENIRDWCISRQIWWGHRIPVFYCQEPGCQHEWASKTAPEKCPKCGTTNFKQDE
                     DVLDTWFSSWLWPFSVFGWPQDNPDLRYYYPTNDLVTAPEIIFFWVARMIMAGLEFMG
                     EVPFRRVYLHGTVRDNQGRKMSKSLGNSIDPLSIIGDFSADALRFSLVALTATGQDVY
                     ISKEKFELGRNFGTKIWNAARFLQMNAGEAPPDVSDPDFTPELLTADDQHILARLHKT
                     IAACDENIERCRFNDYAKAIYDFFWHEYCDWYVEYAKQPLNGTDAARKAEVLKVMHYV
                     FANALRLLHPIMPFLTEELWQGLGYTVLAESVMIASWPRVMEEEELAAWGVQPAAVAY
                     VDAKHAAIGLARNLRADYSLPPSQAADFVLRPADIAIGERVRADRAAYLPYLKAQKLE
                     VEMDFTPAKAMPSIITPLGTLFMSLAGHVDVTAERQRLADQLQKTEQELAAASKRLEN
                     ENFVSRAKPDVVEHARARKRELQAKREKLAKLIEALTPN"
     gene            14966..16126
                     /locus_tag="PKI64_05690"
     CDS             14966..16126
                     /locus_tag="PKI64_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOE00539.1"
                     /translation="MAKELAFVLINPYTVAKSRTGGVIARYISRTGLDFVAARMFAPS
                     AELADAYAAQIRNDAEIDAPIRPFLADYVAREYGPDPATGQRRRVMMLLFEGENAIQL
                     VKDVTGPIRPTNSGETVRDTYGDYILDAAGAIHYLEPAVFIGPSLHAAGETLKLWAKY
                     SVECGGLVESAGDVPQGAATEEALVILKPDNFRFASARPGLIIDIFSRSGLRIVGAKI
                     HRMTVAEAEEFYGPVRDVLREKLKGKVAERAAKAVAAELGMEIPEAIKGQLGELVGPM
                     YGDNQFYQIVQFMTGRWGEGLLPEEKEKPGTLRCLLLVYAGANAISRIRGILGPTDPS
                     KALPGSVRREFGQDIMVNAAHASDSVENARREMGITKVAEETIRPWVEAYYP"
     gene            16171..17373
                     /locus_tag="PKI64_05695"
     CDS             16171..17373
                     /locus_tag="PKI64_05695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOE00540.1"
                     /translation="MFLASIEAAPADPILGLTEAYRKDPRQNKVNLGVGVFMDENGNT
                     PVLESVKRAERRLWESEKTKTYLPISGTPEFDAQVAQLVFGADFHGLAGNRVAVCQSP
                     GGTGGLRIGAELLRNFRRGGTLWLPSPSWDNHQSLFTAAGLTVKSYAYYNPATRGVDV
                     EAMCADLAKIPAGDTVLLHVCCHNPTGADIPAAGWERVADIAAQAGWLPFFDFAYQGF
                     GDGVAEDRAGLLAVLRKAPEALVASSFSKNMGLYAERVGAFSIVAASDKAANAAISQI
                     KRIVRVIYSNPPKHGGMLARLVLEDPELRDLWLQELNAMRQRIAGNRQLLASGLHQRS
                     GQDFSFIVHQKGMFSYSGLSDAQVDYLREEKGIYMVKGGRINVAGLLPRAVDYVCDSI
                     IASLAAHP"
BASE COUNT         3616 a         5346 c         5354 g         3169 t
ORIGIN      
        1 caactcttgc cgcccataac ccccaaactg cgccgttact ttgtcaattg accggctttt
       61 ttgagcatgg tcgccggagg taccgcccgg tctaaacggg catccggctt ttgcccgcgc
      121 ccgtgcccgc cgtggcatag gcggcaggaa tatcgcgtgc ctgccgcacg tctgaaatcg
      181 tgtaaaaagc atcgggatca aacgcatgca cgatggcgcg gagatcattg acccggcggc
      241 gggaggccag aatggaaagc acctgcaccg ggccgcgggt gccctgcgca tcctgccgag
      301 tgacgccata gccagccgcc gacagttgct gcatcaatgg ttcggccggg cgggtggtga
      361 tgacctgtac cagcaccagg cccacggcca aatgttcctc gatgaacatg cccaccagcg
      421 tgccgcaggc atagccggtg gcatacgcca cataaatggc caagtgccgc tcgccggaaa
      481 gcacctgcga aaccacgcaa atccagatga acacctcgat gaacccgcaa caggcggcga
      541 acaccttgcg gccgcgggca ataaacgcca cgcgcatggt gcccatgctg acatcaaaga
      601 tgcgcgccac caccaccatc aggaatggaa acagcagatg catgaaacga atcgcgcgag
      661 gtcggctaga aaggccgcag ggtgaagcgc cgcttccgga atgcggcata atgcgtgtag
      721 ccggcggccc gggcgagggc gacggctggt tcaaagtgct ggccgacatc gtccggattg
      781 tgggcatcgg agccaaagac gatgggtatg tcgcgctcct tcatccagcc aaggatggtg
      841 agggaaggat aagcttcacc cgcgcgatgg cggaagccgc tcgtattgat ttcaatggcc
      901 attccggtgg cggccacggc gtcgagggcg gggggtacaa tttcggccag caaagctgtt
      961 ggcgcatgga tgccggcgcg cttcacgaga tcaaaatgcg cgcaaatatc atacagaccg
     1021 gtttgcgcca tttccttcat gcgttggtaa taggtgcggt tcatctgggc cacgaattcc
     1081 ggcgtggcgt tggggtcatt tggccccaag tcccaaaatg ggcccgtgtg aatggagccg
     1141 agcaccagat caaaatcagc ggcagccaat aggcgcggga tgtgctcgcc aatcaaatcg
     1201 cgctgataat cggcttcaat tcccagcagc acttcgcaga gtccggcttg ctgcgcctca
     1261 cgcaccaatt ccagatagcg gggaaattcg tctgcggtca tgcggatcgc cggtgcggga
     1321 tcgttgggaa atggtatgtg ttccgtacag gcgatttctt tgatgccgcg ggctgccgcc
     1381 gcgcgggcgt agtcgagggg tacgccgccg gcatgacggc acagcgcggt gtgcgtatgg
     1441 gtgtcggctg gcagggtggc ggtcatggaa ggcggaacga taccgcgcgt ggggggcaac
     1501 gtcaacgcgc caccggccgc acgggcgaaa caggtgcgcc gggcgccgcc ggatatgtga
     1561 tggcggccgg ggtttctggt tcgtgcggca cgaacgtgtt ccacaggtag cgcgcgtaaa
     1621 aaccaaagcc caccaacgcg gccaaaccga tcacataaaa aatgactcgc ttgattcggg
     1681 aaggttcaaa ccgggtgcgc cgataggcga tgggcgtgat ggcaatcaaa acgccggccc
     1741 cgagggcaag gccaatgcgg atcagcattt cgtgcagttg gtaggcgggc atcacggggg
     1801 agcactctag cgcgccggca cgcgcgttga caagttagta ttcgaacgcg gagccgccca
     1861 caaattcacg gatgatggag gtgggtggcg gaacatcggc cgccagtggc aggaagtgcg
     1921 agagaaactc cagcattcgc cgcgcctccg cataaatcgg ctgattcggc ttgatttccg
     1981 aaagcagggg tcgcgccaac acggaaagga tggccagttc gtaatccagc gccgagttaa
     2041 aaacggcatc ggcctcttcc tgaaaaggga aaatccaggt gcgttcgcca cggcctacgt
     2101 tgggccacat ttccaatgtg cgcgtggcgg aatgaccgcg gtagcggtgg tcgcgaacca
     2161 agcgccgcag caggcggttg tctgtggtag ggatgcggtt gttgttgtcg agattgagct
     2221 gcgtgagcgc agaaatataa atgtggaact tgcgcccttc cgggatggcc ggagtcagcg
     2281 ccgggttcaa ggagtggatg ccctcaagca ccacaacctg gtcttctgcc agccgcatgg
     2341 tgcgcccttt gtaggtgcgc gcaccggatt ggaaatcgaa tgtcggcaac ttgatttcgc
     2401 gcccggcatc caagtctgcc aagtcgcggt tcaatgtttc cacatccacg gcttggaggt
     2461 gttcaaaatc atattcgccg gtcggggtgc gcggggtatg ttcgcggtcc acgaaatagt
     2521 catccgtgcc aagggtcacc gggcgcaacc cattcacccg caattggatg gcaaggcgtt
     2581 tagcaaacgt ggttttgccg gaggacgacg gccccgcaat gagaatccac ttcaccgtcg
     2641 gagtcgccgc gatttggtcg gcgatcgccg cgattttctt ctcgtgcaag gcctcggcca
     2701 ccttgacgaa gtcgtggatt tcatcggcgg caataatctc gttgagccga cccacggtgt
     2761 tgacattgag aatgcggccc cagcggttgt attccttaaa agtttccgcc aggacggtca
     2821 ccggcttgaa ctccggcaca ttgggggcgg tatccactgt gggcatttgc aggacaaagc
     2881 cggtttggta cggaatgatt ttgaaatagc gcagtgggcc gcaagagcgc gccatggggc
     2941 ccgtggacat atccgagaag ttttcgcaaa cgtaggtgac aatctttggc gtattggtga
     3001 aacgcagcag ggaaaccttg tccatctgcc cgttgcgggt gaattgttcg atggccgccg
     3061 cataggaaat gcgttggcgc agaattgggc ggttggtggt caccagatcg cggatgtgcg
     3121 cttccagcgc ttcggcttgc gcgggggtca tgccacgttg gccattgacc tcaaactcgc
     3181 aatacagccc cggccccaag gcatgctcca ccgagaattg cgcttccggg tgcagatcct
     3241 tcaccgcttt cgccaggaca aaggacagcg accggatata aatctgcatc ccgcccggcg
     3301 tggccatggt cacgaaacgc accgtggcgt caatatccag tgtataagca aacgtcacca
     3361 attcgttgtt caccagcgcc cccaagtagg gcaggccgga gacggggtcg ttcggctcgt
     3421 gcaggagttc gcccaccagc gtttgggagg gacaggtgat tttttggccg gactcgaggg
     3481 tgatggtgat gttctttttc atgtcacccg gatgctgcca tgaccccctt gcgatggaaa
     3541 gcccaaagag gcgcgcggaa ccgtgcgaaa tcggccggca cggcccattg ccctcatgcc
     3601 gccgcctatt cggcgggcga cagcaagtcg tgcacctgaa ttttgcgcga gagggggatc
     3661 agccaatcag catagaactg ggtgatggcg tcaatggtgc cgggcgtcgc ctcggctttg
     3721 cgggcatggg ccgccacgcg ggcgagaaca tccgcctcca ccgcggcgtt ggtgatggcc
     3781 tgctccacga catcaggatt gcgggtggca acggcctgtt gaagcgtttg gggcaatgcc
     3841 tgccgcttgc gggtggccac atggaccgag agcgccaacc gccgcgccat ggcattcagg
     3901 caggcaatat cggcagccaa acaggcttcg cgttccagcg gcgcatcgcc gggtacgcag
     3961 agccgaggaa cgatcacact cagatacggc gcgcgcaaaa gctgcacccg cccggccagc
     4021 gtacgcgact tcgcatagct ggtggccagc gcatccaagc cggcgggcgg cgtctggtta
     4081 acggcataga tggcatcatt ccgcagcaat cgggcgcgcg ctgccaaggc ggccaccagc
     4141 gggccgtcca gcgaggcgat catgtcgcgc aaatgaaaca acgcatccat gcggctattt
     4201 ggcgggatag gtgccgattt tacggccgga ttccgaaagc gcccgcacga gctgccaagc
     4261 cgcctgcgcg gaatcggacg accaatccgt ggcggcttcg atgaaaaagc catattccca
     4321 tggtcgtccc ggcagggggc ggctctggat gaaggtcagg ttgatttctg ccgcatcgaa
     4381 cggtgcgagc acatgcagca atgcgccagg gcgattgccg attaccagat aaattaacga
     4441 ccgtaaccgc gtacttttgc cagccgcttg cggaactttg ccggtggtga gggtgaaaaa
     4501 gcgcgtggta ttttccgggc tgtcttgcag attcttttgc aggatggtca gccgctggat
     4561 acgcgcggca gcggcggagg ccaaggccgc ggtgccgggc tcctgcgcgg cacgcccagc
     4621 ggcaacggct gtgctggatt ccggtaccaa ctcggcgcgt ggcagcaggc gggccagatt
     4681 ttcccggcac tgacccaaca cctgcggatg gctgcatacc cggcggatat ccgccaacgc
     4741 ggtgccgcgc cgcgccatca aatgctgcgg taccggcagc gaaaaactgt ccatgatccc
     4801 gacggtggga tggttggcca attgatccag cgtttgcgtg acgggaccct cgctggagtt
     4861 ttccacgggt accactgctt gtggacggtt tgcgccgcgc gcggtgacca gccgcgaaaa
     4921 aatttcatgg tgcgaaagac atggcaataa cttggccttt tgtccgaaac gcctgatggc
     4981 tgcctcgtgg gagtgcgtgc cttccgggcc aagatacagg ataggagtat tcatgcggtt
     5041 cctcgctctc atcaggacgg ggtggggcgg gtgcgctgct gccgcgccaa ttccagccat
     5101 ggcgccaagt catccatgaa tttttggaaa ttggcggtct ccaactgttg gggagcatcg
     5161 ctgaaggcgc tcaacggatt gggatgagcc tccacgatca ggccatcggc gcccacggcc
     5221 aagcccgcgc gggccaaagc cggcaccagg cggcgcgcgc cggcggcatg gctcgggtca
     5281 acaatcaccg gcaggtggga gagttgcttc gccacggcca ccgcgcccaa atccagcagg
     5341 ttccgggtgc tggaatcgaa tgagcgcagc ccgcgttcgc agagcgcgac ctcggtgcag
     5401 ttgttcacaa tcagatattc cgccgcagtg agccattcct ccaccgtgct ggccagccca
     5461 cgcttcagca agactgggcg cccggcttgg gcaatatgct ccagcaggtc ataattctgg
     5521 gcgttgcgcg cgccaatctg gaacatatcg gccacctcga gcagcggctc gattttgtcc
     5581 gggccgggca cttcggagat catggccaca tcgaactctt gtttgacttc gcgcatgatt
     5641 tccagcccgt cttgtttgag cccttggaag tcatagggcg aggtgcgcgg cttgaagggc
     5701 atggcgcgaa taatccgaat gccgcaggca gtcaaatcct tcacggccgt gcggaactgg
     5761 tcataggact caatggcaca cggccctgcg atgacctcaa tgttgccgcc gccgagcgcc
     5821 acgcccttga cagtcaccac ggaatcgccg gggtgatact cccgcgaaac cagcttgaat
     5881 ttcttttgta cgggaaaaac ggattccacc gccggcagat tgcggaggac ttccagggac
     5941 tggtggctca actcatcgcc gatgcacgcc accacggttt gttccacgcc gcgaataatc
     6001 cgtggctggt agcgcagggt acaaaccatg tcgctgacgg tggcaatgtc ggtttcgctg
     6061 caatttcttt ttaaaacaat aatcatgatt tcaattccga agttaggctg ggggtggctg
     6121 gttcagcggt tgggcgccaa aacgcaaaaa ccgcgcatcc gtggcgccgg acacgcggtt
     6181 gcggaagggg tcccggcagg ggtcaccggg tcgctccgga ccgtgcgtcc ggcgacctaa
     6241 agatgaagcc gagataaccc aagcgcgttt tcatactcgc gcaaactgaa ccatgacccc
     6301 caccggttgt caagcatttg gcctggcaaa aaagtgatct tggccgcggg gggcggccgt
     6361 tgtgctttct gtttctgagt cgtttaccct cagcgaaaac cgcttgccgc cgggcggcgg
     6421 gcagcatagt ctgccctcct gatttacaac caagacccga ttggaatcct gccccatgac
     6481 gacagccaca gacctgcttg ccaaaattaa atcacgccgg gcgcgcctgg ccgtcatcgg
     6541 cttggggtat gtgggcctgc cgttggcggc agagtttggc aagcattacg acgtggtggg
     6601 gtttgatatc aacccccagc gcctgcaaga attgcgcgcc ggaaacgacc gcacgctgga
     6661 agtctccact gccgacctcc gactggccac gcgcctgacc tattccgaca agattgaaga
     6721 tttacgcggc acggatgtgt tcatcgtgac cgttcccacg ccgattgacc gccaccgccg
     6781 ccccgatctg acgccgctgg tcaaggccag cgagaccgtc ggccgcgcat tgcagcccgg
     6841 cggcgtcgtg gtctatgaaa gcacggtcta tcccggttgc accgaggaag attgcgtgcc
     6901 cattctcgaa aaattcagca gcttgaagtt caacgtggac tttttctgcg gctacagccc
     6961 ggaacggatc aaccccggcg acaaagtcca caccgtcacc aagatcaaaa aaatcactgc
     7021 cggctccact ccggagattg gccgcgccgt gaatgcgctc tatgcctcca tcatcaccgc
     7081 gggcacacat ctcgcgccca gtatccgcgt ggcggaggcc gccaaggtga tcgaaaactc
     7141 ccagcgcgac atcaacattg ccttcgtcaa cgaactggcg ctgatttttg cgcggatggg
     7201 attggacacc catgacgtgt tgaccgccgc cagcaccaag tggaactttt tgcctttccg
     7261 ccccgggttg gtgggcgggc actgcattgg cgtggacccg tattacctga cccacaaggc
     7321 ggagtcgctc ggctatcacc ccgaaatcat tttggcgggc cggcggctca acgacaacat
     7381 gggcgcgcac gtggccaacc gcgtggtcaa actgctcatt caaaaaggcc ggcgggtgca
     7441 cggggcgaat gtgctggtgc tgggcgtgac cttcaaggaa aactgccccg acatccgcaa
     7501 ctcgcacgtg gtggacgtgg ttcgcgagtt gcaagacttt ggctgccgcg tggtggtgca
     7561 cgacccatgg gccgatgccg aagaagtccg ccatgaatac ggctttgagc tgctgccggc
     7621 gctggccaaa cccgcgtttt atgagggctt tgacgccgtt attttggcgg tggcccacga
     7681 ggcgtttcgt gatttgacat tcccgcccgc tgaccagcgc aagctggtgg tgtttgacat
     7741 caagtctttc ctcgcacgcg accgggtgga cggtcggctg tagagcgggt ggggtggggc
     7801 ggcgcccaga agatttctcg ggcttgggct tgtttttcgc ccgaagcggg gtattctgtc
     7861 gcacttcata acttgtttat agcgcgtgga gcgttctcat gccgaaaatg aaagaaattg
     7921 gagtggccgt actgggcttt ggcacagtcg gggctggtgt ggtcgaaact ttgcagaaaa
     7981 acggccatct gctggcagac cggctgggca ttcgccttgt cctgcgcggc gtggccgatt
     8041 tggacctgac cactgaccgt ggcgtgaagg tggacccggc gctgctcacc accgatggcg
     8101 ccgccttgat cgcccgcccg gatgtgcaga ttgtcgtcga actcattggc ggtaccggcg
     8161 tggcaaagca gttcattttg caggcgctgc aactgggcaa gaccgtggtg accgccaaca
     8221 aaaaactgtt ggccgaacac ggcgaggaaa tccagcgcgt ggccgatgaa catcaagcgg
     8281 aaatcctctt cgaggcctcc gtgggcggtg gaatcccgat catcaaggcc ctgcgcgagg
     8341 ggctgtgcgc caatcgcatc gagcggattt acggcattct caatggcacc tgcaactaca
     8401 tcctcacccg catggaggag gaaaagctgc cttttgccga ggtgctgaaa gccgcgcagg
     8461 ccgccggcta cgccgaagca gaacccagct tggacattga cggccacgac accgcgcaca
     8521 aagccgcggt gctggcttcg ctggcctatg gcggccatgt gccgctcgcc aaaattcgcg
     8581 tgactggcat ccgacatctg gcggccgccg acattgccta cgccgccgaa atgggctacc
     8641 gcatcaagtt gctggccatc attcagggcg atgccaatgg catcgaagta agcgtgcaac
     8701 caacgctcgt ggcgcacagc caccagttgg ccgccgtggg cggggtattc aacgccgtgc
     8761 tggtgcaggg cgatgtcgtc ggcaccacgc tctactatgg ccgcggcgcc ggtcgcgctg
     8821 ccaccgccag cgcggtggtg gccgatttgg ccgatgccgc cctgaatctg caagccggcg
     8881 gacggcggcg cgacttgagc gccgtgcagt cccgcgcgcc agtggctttc cgcgatccgg
     8941 gcgaaatcgt ggcccgcaac tatctgcgct tcagtatgcg cgacaagccc ggcacgttgg
     9001 ccagcgtggc ttcgatcctc ggccgccacc aaatcggcat tgattccgtg atgcagcacg
     9061 aagcgccgtc gcccgcggac tatgtgcccg tgatcatcgt caccggcccc gcccccgagc
     9121 gggaaatgga tgccgcgctg gcggaaattg cggcaacttc cggcatcgtc aaccaacctg
     9181 cggtacggta ccggattgaa gattttgact aacccgttca gctaacgaag gagaacaaag
     9241 gccatggcct tgtatgtaca gaagtatggc ggcagttccg tggcggatgc cgagtgcatg
     9301 cgccgcgtgg cgcaacgcgt ttttgacacc cagaaagccg gcaaccaggt agtggtggtg
     9361 gtgagcgcca tgggcgacac caccgatgat ctcatcgcgc tggccaagca ggtcaatccg
     9421 gagcccgacg agcgcgaaat ggatagcctg atggccaccg gcgaaatggc ctccagcgcc
     9481 atcctgacca tggcgctgca ttcgctgggc gccaaggcga tttccatgac cggccagcag
     9541 gcgggcatcc aggtggatgg cacgcacctc aaggccaaga tcgaaaaaat caatcccagc
     9601 cgtattcggc agcacctcaa gcagggctat atcgtggtgg tggcgggctt ccagggcatg
     9661 aatccggatg ccgatgttgc cacgctgggc cgcggcggct cggacacgac cgccgtcgcg
     9721 cttgcggcgg tgttgaaggc ggaccgctgc caaatcttga aagacgtgga tggcgtcttc
     9781 accgccaacc cgcgcgtggt gcccaccgcc cgcaagctcg acgaaatcgc ctacgaggaa
     9841 atgctcgaac tcgccagttg cggggccgaa gtgttgcagt cgcgcgcggt ggaattcgcc
     9901 aagaattaca acgtcgtgct ggaagtcatg tcgagttttg aagtcaagcc gggcaccttg
     9961 gtgcgagagg agaacaaaca aatggaacag atgatcattc gcggtatcgc tgccgataaa
    10021 aaccaggcca aagtcacctt gttgagcgtg ccggacaccc ccggcgtggc ggccaagatt
    10081 ttcaaggccc ttggcaacgc caaggtcaac gtggacatga ttgtccaaaa cgtcagcgcg
    10141 gccggcgtga cggacatttc cttcaccgtg gccaaggatg acctcaacaa gaccaagcgc
    10201 gtggtggagc cgctggtgcg cgatatccgc gccaccggcg cgaacttcag cgatgacctg
    10261 gccaaggtca gtttggtcgg cgtaggcatg aagagtcact cgggcgtggc gttcaagatg
    10321 ttcgatgtcc tggcggccaa caagatcaac atcgagatga tttcgacttc cgagatcaag
    10381 ttgtcggtgg tcattctggc caaggataca gaccgggccg tgcgggcgct gcacgaggcc
    10441 ttcgggatgg gtgtgcgcgc gcgccaagcc aaggccgcgc ccaaaaagaa aacggcccgc
    10501 cgctaaccgg cgcatggggc tcatggccca aggagagaca cgatgaaaaa ggtgagcatc
    10561 tacgacacca ccctgcgcga tggcgcccag ggcgaaggca tcaatttctc cctgcccgcc
    10621 aaggtgcgca ttgcccgggc gctggatgag ttccgcattg attacatcga gggcggcttt
    10681 gcggcctcca accccaagga catggctttc tttgaggaaa tgaaaaaggt caagctgcgc
    10741 cacgccaaac tggcggcgtt tggcagcacg cggcgcgcca gcaaaaccgc ggagcaggat
    10801 ccgggcctcg ccgcgctctt gcaagcgggc actcccgtca ccaccatctt tggcaaaagc
    10861 tggctgctgc atgtggaaaa ggtgctcaag accaccgccg ccgaaaacct gaagatgatc
    10921 gccgattccg tggcttacct gaaaagccac ggcaaggaag tcatctatga cgccgagcat
    10981 tttttcgacg gctatctgga caatgccgac tatgcgctgc aaacgctgca agccgcggct
    11041 gatgccggcg ccgatgccct tgtgctgtgc gacaccaacg gcggtcggtt gacctccgaa
    11101 gtagtcagca tcacccaagt ggtggccgcg cgcttcccaa aggcgcgcat tggcatccat
    11161 acgcataacg atgccgagtt ggccgtggcc aacagcttgt ccgccgtcga gagtggcgcc
    11221 acgatggtac aggggaccat gaatggctat ggcgaacgca ccggcaacgc caatctgtgt
    11281 tccatcattc ccggcttggc cttgaagctg ggcgccaagc tttcctgcgg ggcgcgcctg
    11341 caaaaactgc gcagtatttc cgtgctggtg gatgaaatcg ccaacgtgcg ccccttgaag
    11401 agccggccct atgtcggcca gagcgccttc gcccacaagg cggggatgca cgtggatgcc
    11461 gtcagcaagg tctcccgcag ctttgagcac attgaaccgg aatccgtcgg caatgagcgg
    11521 cggattttga tgagcgaact ttccggcgcg agcaacgtgc gcctcaaaac cggcgaaatg
    11581 ggcctggagt tccaaaagga tgcgccggcc atcaaggaca tcctgcacaa gctcgaacat
    11641 ctggaaaaga acggctacgc cttcgagtcg gcggatgcgt cgttcaagct gctcgtgcaa
    11701 aaggtgttga agaaacacgt gccgtttttc gagctgcagg gcttcagcgt cgtggtgcaa
    11761 aagcgcgatg ccgacagcaa ggccaccact accgccacca tcaagatcag cgtcaagggc
    11821 aagaccgagc tgaccgcggg cgagggcgat ggcccagtgg atgcgctcaa caacgcgctg
    11881 cgcaaggtgc tgcaccagtt ctatccacaa ctggattccg tggtgctgca ggattaccgc
    11941 gtgcggattc tcaaccccga aaccgccacg caggccacca cgcgcgtctt gattgaatcg
    12001 tccgacggcc gcgaacggtg gggcaccgtg ggcgtcagcg agaacatcat cgaagcctcc
    12061 tgggaagcat tggtggacag catggaatac aagctgttcc tcgacgaaca gcggcggcaa
    12121 aaacgccagc gcaaataacc cccagccgca tcacctgcgg cggcttgccg ccgggcccag
    12181 gcggaagaaa atactttcca aaaacggcgt tttggccgat agttcgggcc attttacaaa
    12241 ggatttttcg caatgtcaga gttacccaaa acctacgacc cgcaggccat cgaggccaaa
    12301 tggtacgact ggtggaagca gcgcggcttc ttccgggccg aggccgagtg gggggggcag
    12361 ccgtacacga ttgtgatccc gccgcccaac gtgaccggca tcctgcacat gggccatgcc
    12421 ctgaacgaaa ccatccagga cgtgatgatc cgccgcaagc gcatgcaggg cttcaacacg
    12481 ttgtgggtgc cggggactga ccatgccggc atcgccacgc aaaacgtggt ggagcgccaa
    12541 ctgaaagagc agggcaaaag ccgctgggac atgtcccgcg aggaattcct gaaactggtt
    12601 tgggcctgga aggagcagta tggcggcact atcctgcgcc aactggaaaa actcggcaat
    12661 tcctgcgact ggtcgcggac ccgcttcacc atggatgagg gcctcagcga ggccgtggcg
    12721 gaaaccttcg tgcggctcta cgacaaaaag ctgatctacc gcggcgaata catcatcaac
    12781 tggtgcccgc gctgcgagac tgcgctctcc gacgaagaaa gcgagcacac cgagcaggcc
    12841 ggccggttgt atcacatccg ctaccccgtc aagggcgggc gcaagagcga atatgtggtc
    12901 gtggccacca cccgcccgga aaccatgctg ggcgataccg ccgtggcggt gaacccgcgt
    12961 gacgaacgct atgccaagtt caagggaaaa acactggtgc tgccaatcct gaatcgcgaa
    13021 atccccgtga ttgaagacga ttttgtggat cccaagttcg gcaccggcgc ggtgaaagtg
    13081 acccccgcgc atgatcccaa cgacttcgac atgggccgcc gccacgcgct gccgatgatc
    13141 aatgtgatga ccccccgcgc gcagatgaac gaaaacgccg ggccctatca ggggatgtcg
    13201 cgcgaagaat gccgcaagaa actggtggcc gacttgcagg cggcgggctt gctggtcaag
    13261 attgacgagc acgcgcacgc ggtgggccat tgctaccgct gccacacggc cgtggagccg
    13321 cgcctctcgc cgcagtggtt cgtcaaaatg aagccgctgg cacgccccgc cattgatgcc
    13381 gtcaaggatg ggcgcatccg ctttgtgccg gaacgctgga gcaaggtata cctggaatgg
    13441 atggaaaaca tccgcgactg gtgcatcagc cgccaaatct ggtgggggca ccggattccc
    13501 gtgttctatt gtcaggagcc cggctgccag cacgaatggg cgagcaagac cgcgccagaa
    13561 aaatgcccga aatgcggcac caccaacttc aaacaggatg aagacgtgct ggacacgtgg
    13621 ttcagctcat ggctctggcc gttcagcgtg ttcggctggc cgcaggacaa ccctgacctg
    13681 cgctactatt atcctaccaa cgacctcgtg accgccccgg aaattatttt cttctgggtg
    13741 gcgcgcatga tcatggccgg cctggagttc atgggcgaag tgcccttccg ccgggtctat
    13801 ctgcacggca ccgtgcgcga caaccaaggg cgcaaaatga gcaagagcct gggcaattcc
    13861 attgacccgc tcagcatcat cggcgacttc agcgccgatg ccctgcgctt cagtttggtc
    13921 gcgctgaccg ccaccgggca ggatgtctat atctccaagg aaaagttcga gttgggccgc
    13981 aatttcggca ccaagatttg gaatgccgcg cggttcttgc aaatgaacgc cggcgaggcc
    14041 ccccccgacg tgagcgatcc ggacttcacg ccggaactgc tgaccgccga cgaccagcat
    14101 atcctcgccc ggctccacaa aaccatcgcc gcgtgcgatg aaaacatcga gcgctgccgg
    14161 ttcaacgatt acgccaaagc catctatgat ttcttctggc acgagtattg cgactggtat
    14221 gtcgagtatg ccaagcagcc gctcaacggc accgatgccg cccgcaaggc cgaggtcctc
    14281 aaggtcatgc actatgtgtt cgccaacgcc ctgcggctgt tgcatcccat catgcccttc
    14341 ctgaccgaag aactctggca ggggctgggc tataccgtgt tggcggaatc cgtcatgatt
    14401 gcctcgtggc cacgcgtgat ggaagaagaa gaactcgccg cctggggcgt gcaacccgcg
    14461 gccgtggcct acgtggatgc caaacacgcc gcgattggcc tcgcccgcaa cctgcgcgcg
    14521 gactacagcc tcccacccag ccaagccgct gacttcgtgc tacgcccggc ggatatcgcc
    14581 attggcgagc gcgtgcgcgc cgatcgcgcg gcttacctgc cttatctcaa ggcgcagaag
    14641 ctggaagtcg aaatggactt tacccccgcc aaggccatgc ccagcatcat cactccgctg
    14701 ggcacgctgt tcatgtcgtt ggccggccat gtggatgtca ccgccgaacg ccagcgcctc
    14761 gccgaccaac tgcaaaagac cgagcaggaa ctggccgcag ccagcaagcg gctggaaaac
    14821 gaaaactttg tcagccgcgc caagcccgac gtggtcgaac atgcccgcgc ccgcaagcgg
    14881 gagttgcagg ccaaacggga aaagctcgcc aagttgattg aagccctgac ccccaactga
    14941 accgccaccg agaaaaggat tccccatggc caaagaactc gcttttgtat tgatcaatcc
    15001 ctacaccgtc gccaagtcgc gcaccggcgg cgtcatcgcg cgctacatca gccgcaccgg
    15061 cttggatttc gtggccgcgc gcatgtttgc gcccagcgcc gagcttgccg acgcctatgc
    15121 cgcgcaaatc cgcaacgatg cggaaattga tgcgcccatt cggccttttc tggcggatta
    15181 cgtcgcgcgc gaatatggcc cggacccggc caccggccaa cgccgccgcg tcatgatgct
    15241 gctttttgaa ggcgagaatg ccattcagct cgtcaaggat gtcaccggtc ccattcgccc
    15301 caccaacagc ggcgaaaccg tgcgcgatac ctacggcgac tacattctgg atgccgccgg
    15361 cgcgattcac tatctcgaac ccgccgtgtt catcggccct agcctccatg ccgccggcga
    15421 aaccctcaag ctgtgggcca aatacagcgt ggaatgcggc ggcttggtcg agtccgccgg
    15481 cgatgtcccg caaggcgccg ccaccgaaga agcgctggtg attctcaagc ccgacaactt
    15541 ccgcttcgcc agcgcccgcc ccggcctgat cattgatatt ttctcccgct ccggcctgcg
    15601 catcgtcggc gccaagattc accgcatgac ggtcgccgaa gcggaggagt tctacggccc
    15661 cgtccgcgat gtcctgcgcg aaaagctgaa aggcaaggtg gccgagcgcg ccgccaaagc
    15721 cgtggccgcc gaactcggca tggaaatccc cgaggccatc aagggccaac tgggcgagct
    15781 ggtcggcccg atgtatggcg acaaccagtt ctatcaaatc gtgcaattca tgaccgggcg
    15841 ttggggcgaa ggcctcctgc ccgaagaaaa agaaaagccc ggcacgctgc gttgcctgct
    15901 gctggtctat gccggcgcca acgccatttc gcgcatccgc ggcatcttgg gccccaccga
    15961 ccccagcaag gcgctgcccg gctctgtccg gcgcgagttc ggccaggaca tcatggtcaa
    16021 cgccgcgcac gcttccgact ccgtcgaaaa cgcccgccgc gaaatgggca tcaccaaggt
    16081 tgccgaagaa accatccgcc cgtgggtcga ggcctactat ccataagaca agcgtcctaa
    16141 aaaaaataaa taaccgccag aggatacgcc atgtttctcg cttccatcga agccgcgccc
    16201 gccgatccga ttctcggcct gaccgaggcc tatcgcaaag acccacgcca gaacaaagtc
    16261 aatttggggg tgggggtgtt catggatgaa aacggcaaca cgcccgtcct ggaaagcgtc
    16321 aaacgcgctg aacgccgcct ttgggaaagc gaaaaaacca agacctatct gcccatttcc
    16381 ggcaccccag aatttgacgc gcaagtggcc cagttggttt tcggcgccga cttccatgga
    16441 ctcgccggca accgcgtggc ggtttgccaa tcacccggag gcaccggcgg cctgcgcatc
    16501 ggcgcggaac tgctcagaaa cttccgtcgc ggtggcacat tgtggctgcc gtcgccctca
    16561 tgggataacc accagagttt gttcaccgcc gccggcctca ccgtaaaatc ctatgcctac
    16621 tacaaccccg ccacccgcgg cgtggatgtg gaggccatgt gcgccgatct cgccaagatt
    16681 cccgccggcg acaccgtcct gctgcacgtc tgttgccaca accccaccgg cgcggacatc
    16741 cccgccgccg gctgggaacg cgtggccgat atcgccgccc aagccggctg gctgccgttc
    16801 ttcgacttcg cctatcaagg ctttggcgac ggcgtggcgg aagaccgcgc cggcctgctg
    16861 gccgtcctgc gcaaagcgcc cgaagccttg gtggcctcct ccttctcaaa aaacatgggc
    16921 ctctacgcgg agcgcgtcgg tgcgttttcc atcgtggccg ccagcgacaa agccgccaac
    16981 gccgcgattt cgcaaatcaa gcggattgtg cgcgtcatct actccaaccc gcccaaacac
    17041 gggggaatgc tcgcccggct ggtgttggaa gaccccgaac tgcgcgacct ctggttgcag
    17101 gaactcaacg ccatgcgcca acgcattgcc ggcaaccgcc agcttctggc ctccggcctc
    17161 caccagcgca gtggccagga tttctccttc attgtccacc aaaaagggat gttctcctac
    17221 agcggcctca gcgacgccca agtggattac ctgcgcgagg aaaaaggcat ctacatggtc
    17281 aagggcggcc gcatcaacgt cgccggcctc ttgccccgcg ccgtggatta cgtctgcgat
    17341 tccatcatcg ccagcctcgc cgcccatcca tagacggcat tccgacaggt tttccacagc
    17401 gtggaaatat ttttatcccg cattggcggg attttccaca gataggatga gaaggcaaaa
    17461 gaggatcggt ttcctttggg cgttg
//
LOCUS       DAOPNP010000046        17417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3893_length_17417_cov_8.759187, whole genome shotgun sequence.
ACCESSION   DAOPNP010000046 DAOPNP010000000
VERSION     DAOPNP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3893_length_17417_cov_8.759187"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(815..1717)
                     /locus_tag="PKI64_05700"
     CDS             complement(815..1717)
                     /locus_tag="PKI64_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOE00541.1"
                     /translation="MNKPTLLILAAGMGSRYGGLKQMDPVGPAGEIVLDYSIFDALRA
                     GFGRVVFVIRRELEAAFRAQVSAKWESRIPCHYAFQELSDLPPGWTVPADRAKPWGTA
                     HAIRAGRHAIQGPFAAINADDFYGATSFQALARHLMADAPATAHCMVAFRLDQTLSAH
                     GSVSRGVCLHNTAGQLTGIREHTKITRHPDGTLWDEPADAPPAALTGTEPVSMNCWGF
                     GPAIFAELENAFTEFLRVGGQSPQKEFTIPAVVDGLIRGGRGTVQVLPTTSRWFGVTY
                     REDKAAVEAAIRDLIATGDYPSAL"
     gene            1843..2718
                     /locus_tag="PKI64_05705"
     CDS             1843..2718
                     /locus_tag="PKI64_05705"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOE00542.1"
                     /translation="MKIGIVANLSKPAAAATLAALAAAARELRVSFVVGDPEVRRHLP
                     QAKLVAPARFGKAVDVLLSLGGDGTVLQAVRALNGASAPILGINLGNLGFLTSVPDAQ
                     AIAALRAIVSGAHEVVGYPLLEARLLRGPRRRLVAKARALNDIVMGWGTSPRVTMIEV
                     EVDHQPVTSFVCDGLIVATPIGSTGHALSAGGPILHRETPALVLEPICPHTLSNRPVV
                     LPNDRLVSLRVADVRKKLLLSVDGQSSGWLEYGDVLEIRQSPDVVRFVHLPGYSWFKL
                     LSQKLHWRGSSADPK"
     gene            3011..3613
                     /locus_tag="PKI64_05710"
     CDS             3011..3613
                     /locus_tag="PKI64_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE00543.1"
                     /translation="MPSANTRMANITFVIFDTETTGFSPAKDRIVEIGVVKVRDGKVL
                     GEKSWLINPQRYIPYYVQNVHNISSDMVKDCPTFAEVYPEFLKFIDGAVLIAHNAPFD
                     IRFIAAEAERNHMPAPKNAVLDSLTLFRNWYPNLPSHRVEDLINFFDISTDGLQAHRA
                     TDDSLFVYYAIQKEMERRSAEPRFQELLQSAGNALRFTGN"
     gene            3694..4488
                     /locus_tag="PKI64_05715"
                     /pseudo
     CDS             3694..4488
                     /locus_tag="PKI64_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            4531..5064
                     /gene="msrA"
                     /locus_tag="PKI64_05720"
     CDS             4531..5064
                     /gene="msrA"
                     /locus_tag="PKI64_05720"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOE00544.1"
                     /translation="MPAPPPPVELATFAGGCFWCIEEIFRQQPGVRRVTSGYTGGETT
                     NPTYRDVCSGETGHAEAVQIEFDPAQTSYAALLDLFLRAHDPTQLNRQGADVGTQYRS
                     AIFTHGPAQAEAARAALAAANASGQFTGPIVTQIEPAGPFYPAEADHQEYYLRNKSAP
                     YCRMVIRPKLNTLGLQQ"
     gene            5211..5645
                     /gene="rpiB"
                     /locus_tag="PKI64_05725"
                     /pseudo
     CDS             5211..5645
                     /gene="rpiB"
                     /locus_tag="PKI64_05725"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
     gene            5793..6485
                     /gene="deoC"
                     /locus_tag="PKI64_05730"
     CDS             5793..6485
                     /gene="deoC"
                     /locus_tag="PKI64_05730"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOE00545.1"
                     /translation="MPNLAPAQLARLIDHTLLKPEATQAEIKQLCEGALKHNFYSCCV
                     NSVWVRYVAQILRGSSVKVCAVVGFPLGAAPPEIKALEARRAIREGAKEIDMVMNVGL
                     AKAGDWDAVTRDIRAVSEACKDGGALLKVILETCLLTKDEIVKACEASMKARADYVKT
                     STGFNKGGATAEDIALMARTVAPKRLGVKASGGVRSYADAMLMIRNGATRVGSSNSVK
                     MMEEAAAACGGK"
     gene            6498..7133
                     /locus_tag="PKI64_05735"
     CDS             6498..7133
                     /locus_tag="PKI64_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00546.1"
                     /translation="MAELRTYIFIDSLQPQFASFLATVARGYLPVAGQAALFVEISPG
                     IAINQVTDVALKSTRVTPGMQIVERLYGMLEIHSDSQADVRQAGAAILKDLDLSEDQR
                     IKPKVYSSQVIRNIDDHQTMLINRMRHGNMITRGQSLFIMEVAPAGYAALAANEAEKA
                     ANINVLEVITFGSFGRIWLGGEERDIDVAYRAAEAAIEAVSGRAEGAGGRS"
     gene            7170..7466
                     /locus_tag="PKI64_05740"
     CDS             7170..7466
                     /locus_tag="PKI64_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOE00547.1"
                     /translation="MADALGMIETRSFPATVEAADAMVKAAKVELVAYERTGGGFTSV
                     IVRGDVAAVKAACDAGQIAAGRVGEVVAVHVIARPHANVDAVLPLGRVATKPAK"
     gene            7566..7853
                     /locus_tag="PKI64_05745"
     CDS             7566..7853
                     /locus_tag="PKI64_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOE00548.1"
                     /translation="MNLGKVVGTVVSTRKESVLDGIRFLLVRLVDTDGKETSSFVVAA
                     DAMGAGPGEMVLTAAGSSARQTTMTQNKPVDNVIMAIVDTWEVNGVTKYTK"
     gene            7907..9358
                     /locus_tag="PKI64_05750"
     CDS             7907..9358
                     /locus_tag="PKI64_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOE00549.1"
                     /translation="MKLSDQDIEAIARRIAGDLRGSPAPAAAAPAAAPAPAAFADGAM
                     GVFRTVDEAVAAAKAAFPVFSSLCLSKRGEIIASIRAVMRENATALAKAAFDETGYGR
                     FEDKIQKNQLVIEKTPGTEDLLPSAWTGDHGLTLMEPAPFGIIGAITPTTNPTSTIIC
                     NAIGMLAAGNVVVFNVHPSARQCSIQTVALLNKAITAAGGPPNAVVCVGESSIESAGQ
                     LMKHPGIRVLVVTGGGGVVKAAMASGKRALCAGPGNPPVVVDETADIEKAASDIVYGA
                     SFDNNMICVEEKETVVVASVADKLIQAMLRNNAVLIDKSKIAAMEQTIFTKMAGPRGH
                     AEINRKWIGQNADKILAAMGISAPATTRLAIVELPEDHPLFWTEQMMPVMPICRVPNV
                     DYAIDIALQMEGHNYHTALMHSRNIDKLSKMARQCNCSIFVKNGRSQAGLGFGGEGYT
                     SFTIASPTGEGLTTPRSFSRLRRCALIDHFRIV"
     gene            9420..10034
                     /locus_tag="PKI64_05755"
     CDS             9420..10034
                     /locus_tag="PKI64_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOE00550.1"
                     /translation="MKPHPAFAVIEFNSVPVGLFAADALLKKSPISLIRSGTIGAGRF
                     LILLAGTTASVAEAHEEALFHGGLQVADDVFLPDIHPALYDGILGNVRNVGDGPVLVL
                     ETPTVSCNIQAVERAMKGVPVDLLEFRAGDPRMDGRGLAILQGDLYDIEAAQEIALAT
                     LETRGIPAQHRILTAPHDALLAQLSVSTRFDLSKPLALEGETAS"
     gene            10052..10342
                     /locus_tag="PKI64_05760"
     CDS             10052..10342
                     /locus_tag="PKI64_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOE00551.1"
                     /translation="MLIGKVIGTVVSTQVYEGLAGVPMLLVQPLTKEGVAKGEPIVCA
                     DGTRMAGPGETVYYEGGREAAMTLNPWFVPVDHAIVGIVDGVDAPNWKGGPT"
     gene            10339..10614
                     /locus_tag="PKI64_05765"
     CDS             10339..10614
                     /locus_tag="PKI64_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOE00552.1"
                     /translation="MIIGKVIGNLHSTINHPFYDGKKILVVERIETDGKPAAGYLVAL
                     DRAGAGPGETVLILDEGNGARQVFESADGPVRSVIVGIVDEIATTPA"
     gene            complement(10663..10884)
                     /locus_tag="PKI64_05770"
     CDS             complement(10663..10884)
                     /locus_tag="PKI64_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOE00553.1"
                     /translation="MPSLRIFSGEALCRLLEREGFVLVRQRGSHAVMQKHIGAQTRTV
                     PIPLHREVKRGTLQSIIRQSGLPRAVFEK"
     gene            complement(10877..11101)
                     /locus_tag="PKI64_05775"
     CDS             complement(10877..11101)
                     /locus_tag="PKI64_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE00554.1"
                     /translation="MKRMTFTALLEKEASGYTALCPELDVASQGDSVEEALANLREAV
                     ELFLECASEAEIKERLREEVFVTRFEAAYA"
     gene            11330..12508
                     /locus_tag="PKI64_05780"
     CDS             11330..12508
                     /locus_tag="PKI64_05780"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOE00555.1"
                     /translation="MKAILIILDSVGIGAAPDAAEYGDAGSATLPHLAAAVGGVNLPT
                     MQKMGLGNIPPLLHHRPPLTIAGVPPVEKPIASYGAMREMSDGKDTITGHWEIAGLEV
                     DPGFHNFPFENSFPPDLIAAFEKETGRKLIGNKAASGTEIIKELGEEHLQTGALICYT
                     SADSVFQIAAHEEIVPVPELYRYCEIARRLCDPLRVGRVIARPFIGQPGSFKRTENRK
                     DFAYLPDEKTILERLTEKGIPVYAVGKIEDIYAHRGITESVHTGNNADSQALVEKWTK
                     EKDNGLIIANFIDYDMLYGHRRDAKGYADSLEKTDQWLANYLPLLGADDMLIVTADHG
                     NDPTFQGTDHTREYVPLLVYQPGEPVENLGVRKGFYDIAQSLARYFSIPALPRGASFV
                     "
     gene            complement(12577..13167)
                     /locus_tag="PKI64_05785"
     CDS             complement(12577..13167)
                     /locus_tag="PKI64_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE00556.1"
                     /translation="MALALRIKFRLRVTGRQYVPAKGGAVIAANHCSYLDPPVMAACS
                     ARRIVHFMARDTLYSNAMARWFFPRVGVIPLDRTRGDLGALKKALAMLKENRVIGLFP
                     EGTRSPDGQMREAKGGIGFLIAKAAVPVVPLYISGTFAAFPKGASKFRPARITARLGP
                     AIQPDEILAAMPGKGAYEAVGALVMSRIRQLAEASE"
     gene            complement(13223..13909)
                     /gene="cmk"
                     /locus_tag="PKI64_05790"
     CDS             complement(13223..13909)
                     /gene="cmk"
                     /locus_tag="PKI64_05790"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOE00557.1"
                     /translation="MNKQSIIVAVDGPSASGKSTVSRRTAQAVGFIYVDSGALYRGIT
                     WKCVKEGIDVKDTQAVTDLLIRMNMELVLRDNAVGYLIDGEDPGQEIRAEPVRERVAD
                     IAAQQVVRVYIVAKLRLMTKFGNLVMEGRDIGTVVFPDTPFKYYLDADPEERARRRAA
                     EIKQAEGTDDVAAVLSSLKRRDQKDSSRATAPLQIALGAKIINSTGMTIEQVVDTIVT
                     DLKQAGVVGS"
     gene            complement(13902..15224)
                     /gene="aroA"
                     /locus_tag="PKI64_05795"
     CDS             complement(13902..15224)
                     /gene="aroA"
                     /locus_tag="PKI64_05795"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOE00558.1"
                     /translation="MAIPKQTKIVYPGGFDGGVVRVPGDKSISHRTALLAGISSGTSE
                     IRGFLQAEDCLNTLKAMESFGARSWVDEEGVLVIQGTGGKMIEPVGPLDVGNSGTGMR
                     LLAGLCAGSPITVTLTGDASLSGRPMRRIKEPLELMGATVELTPQGTAPMTIHGGNLH
                     GIEYCLPTASAQVKSCCILAGLYANGSTTVVEPIPTRDHTERMLRAVGAPIRVEGQRI
                     SVDGYGPKGPKFLARPYVVPGDFSSAAYWLAAAAAIPGITVRAEGIGLNLRRTALLDV
                     LRRMGARVEIERTSAPTDPEVYGNVTVYGAELKGTEVGGAEIPNLIDELPLVAVLGAL
                     AKGRTVIRDAGELRVKESDRVAVMVENLTNLGAHATEQEDGMIIEGPTKVSPIKSVRS
                     YGDHRIAMSMSILAIFADQPVTIANVACVDTSYPEFWEQLAKLGVKVE"
     gene            complement(15265..16131)
                     /locus_tag="PKI64_05800"
     CDS             complement(15265..16131)
                     /locus_tag="PKI64_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOE00559.1"
                     /translation="MTQEAGISIVGLGLMGASLAMALRKRGYAGRLVAYARREETRQE
                     AVARGIVDAAYADPDAAIAGTTLIVLCVPIRSCVEFAGELAPLLQPGMLVTDVGSTKS
                     WICRQMAGLLPPGTFVGSHPIAGSEKQGLQAASADLYANALTVLCSHLDAPEPAVARV
                     AALWQAAGARTCRMEPDKHDRLLARTSHLPHVAAAALAKAIGRDCAEQVGAFCGTGFY
                     DSTRVASGSIDMWHDILLTNAAAVAEELRAFKIEVEQVYDDLQAGRFGDVAAFLERAR
                     VARAELLKGRGR"
     gene            complement(16139..17176)
                     /gene="aroF"
                     /locus_tag="PKI64_05805"
     CDS             complement(16139..17176)
                     /gene="aroF"
                     /locus_tag="PKI64_05805"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE00560.1"
                     /translation="MIVVMKKGATEAEVEALTAAVAEWGLKAHVSPGTEVTVIGLIGD
                     AARDHEATLALMPGVDKVLPVLKPYKRVSTEFHPEKTVIKIPPARHGDRGLSIGGTGV
                     VVMAGPCAVESREMILSLGAFLKGAGAHLLRGGAFKPRTSPYAFQGLGVEGLRYLAEE
                     RAVVGLPVITEVMDPRDVEMVGEFADIIQVGARNMQNFNLLKAVGRCGKPVMLKRGLA
                     NTLEELLMSAEYVVSAGNPEVILCERGIRTFEKFTRNTLDLSAVPVLKRESHLPVVVD
                     PSHGVGQWDLVAPMAKAAVAAGADGLIIEVHPDPAQAVSDGAQSLTPKRFKELLGALE
                     LVARAVGRNLH"
BASE COUNT         3338 a         5726 c         5225 g         3128 t
ORIGIN      
        1 agtgttcatc gggtgatgac gcaaccccta tgcccaaatg catttttcgg gattatctta
       61 ccgcatcgcc gaaggctgcg cgagggtgag cggaaaacaa aagtccgggc cgagccttgt
      121 ccgctgcgcg agcacatggc tgctccctga actctgaacc ctgacctctg acctctccgc
      181 cgagcgcccc gtgaggcggg gcggcgcgcc agcgcggaaa agacgccggt tggttttggc
      241 gccgccaaaa tccacaagaa atcctccacc ccaaacgccg gaagatggtt gcaggttcaa
      301 ggttgcaggt tacaggagaa cagcctgaac agccctgggc ttaacgagcc cggctacagg
      361 aatagccaca cagcctgaag ccaactgtgc ggaagacatc tcgctaaaaa tctgccgcgc
      421 tgcccgaggc gaagtggaaa tcaaaaaggc catgccgggc cttgtccgct gtgcgcagca
      481 cgtgcatgcg gaggatttct tgcggatttt ggcggggttc tcacgccaaa ataagcggcg
      541 gattttccga tgatggcgcg ttgcggacga cccgagctgg actcgtgtcc gcgaggggtc
      601 ggtcgcggcg cgccagcgcg gaaaagacgc cggttggttt tggcgccgcc aaaatcctca
      661 agaaatcctc cattcaaaac gccggaacag acagagccca ctagccaaag ccacgaacca
      721 acagcccaac agccgcaaca gccagaacag agccccgggc tcagcgagcc cggctacaga
      781 aacagccaaa cagccggtcg ccaacgaagc ggcgctagag cgcggaggga tagtcgccgg
      841 tggcgatgag gtcgcggatg gccgcttcaa cggcggcttt gtcttcacgg taggtgacgc
      901 caaaccaccg ggatgtggtg gggaggactt gaaccgtacc gcgaccgccg cgaataagac
      961 catccaccac ggcgggaatc gtaaattcct tttgggggga ttggccgccg acgcggagaa
     1021 attcggtgaa ggcgttttcc agttcggcga agatggccgg gccgaagccc cagcaattca
     1081 tggaaaccgg ttcggtgccg gtgagagcag cggggggggc gtcggcgggt tcatcccaaa
     1141 gggtaccgtc ggggtggcgg gtgattttgg tatgctcgcg gatgccggtc agttggccgg
     1201 cggtgttgtg aaggcatacg ccgcggctaa cgctgccgtg ggcggagagg gtttgatcca
     1261 aacggaaggc gaccatgcag tgggcggtgg cgggggcgtc ggccatgagg tgccgggcga
     1321 gggcttggaa ggaggtggcg ccatagaagt catcggcgtt gatggcggcg aaggggcctt
     1381 ggatggcgtg gcggcccgca cggatggcat gcgcagtacc ccagggcttg gcacgatcgg
     1441 cgggaaccgt ccagccgggg gggaggtcgc tgagttcttg aaaggcgtag tggcagggga
     1501 tgcgggattc ccacttggcg ctgacttgcg cgcggaaggc ggcttccagc tcgcgacgga
     1561 tgacaaacac cacgcggcca aagccggcgc ggagggcgtc gaagatggag taatcgagga
     1621 caatttcgcc ggcggggccg acggggtcca tttgcttgag gccgccgtag cggctgccca
     1681 tgccggcagc cagaatcaac agggtgggtt tgttcatggg gttagtgaag cacaaatggg
     1741 cgggcggctg aaactttttc gtgcgcgtgg cggggaccgc attcaggatt gaaaaagcgc
     1801 ggcgcttggg ggatatgtag gccgcaagag cggagtgact ccatgaaaat cgggattgta
     1861 gccaatttat ccaagccggc ggcggcggca acgctggccg cgctggcagc ggcggcgcgc
     1921 gagctgcgcg tgagttttgt ggtgggcgat ccggaggtgc ggcgtcatct gccgcaggcc
     1981 aagttggtgg cgccggcgcg cttcggcaag gcggtggatg tgctgctctc gttgggggga
     2041 gatggcacgg tgctgcaggc ggtgcgggcg ctcaacggcg ccagcgcgcc gatcttgggc
     2101 atcaatttgg gaaatctggg atttttgacc agcgtgccgg atgcgcaggc cattgcggcg
     2161 ctgcgggcga ttgtgtccgg cgcgcacgag gtggtgggct acccgctgct ggaggcgcgg
     2221 ttgctgcggg ggccgcgccg ccgtttggtg gccaaggcgc gggcgctgaa tgatattgtc
     2281 atgggctggg gaacatcgcc gcgggtgacg atgatcgagg tggaagtgga tcaccagccg
     2341 gtgacgtcgt tcgtgtgcga cgggctgatt gtggccacgc ccattggcag cacgggccac
     2401 gcgctctccg ccggcgggcc gattctgcac cgcgaaacac cggcgctggt gctggagccg
     2461 atttgcccac acacgctcag caaccggccc gtggtgctgc ccaacgaccg cctggtttcg
     2521 ctgcgcgtgg cggatgtgcg caaaaaactg ctgctctcgg tggatggaca gtcgtccggc
     2581 tggctggaat atggcgacgt gctggaaatc cgccagtcgc cggatgtggt gcgctttgtg
     2641 catttgccgg gctactcgtg gttcaagctg ctttcgcaaa agctccactg gcgcggctcc
     2701 agcgcggacc ccaaatagag cgcgccccct cccccccgcg ggcggcattt taccgttttt
     2761 tattcgcatt ttaggcgttg acccgcccgc gccgaatggg tagcttggtt gacagtgaat
     2821 ggcgcgctga ttgggcgttg ttcattagtt gctccgagga acggcggaat ttggcaacga
     2881 agaacggaac cgaaaggagg ccgttatgag tcgaaaagtg ccacggcata tcgtttggat
     2941 atgctcgttc ctcatcatat ttgcagcggg cgccgcggaa tccaccccgg tgaccccacc
     3001 ggcgtttcgc atgccctcgg ccaacacccg gatggccaac atcacgtttg tgatttttga
     3061 cacggaaacc acgggcttca gcccggccaa ggaccgcatt gtggaaattg gcgtggtcaa
     3121 ggtgcgcgac ggcaaggtgc tgggcgaaaa atcctggctc atcaatccgc agcgctacat
     3181 tccctactac gtgcaaaacg tgcacaacat cagcagcgac atggtcaagg attgccccac
     3241 gtttgccgag gtttatcccg agtttctgaa gttcattgat ggcgcggtgc tgattgccca
     3301 caacgcgccg tttgacatcc gcttcattgc cgccgaagcc gagcgcaacc acatgcccgc
     3361 gccgaagaac gccgtgctcg acagcctgac gctcttccgc aattggtacc ccaacctgcc
     3421 ctcgcaccgc gttgaggatt tgatcaactt cttcgatatt tcgaccgacg gcctgcaagc
     3481 ccaccgcgcg acggacgact cgctgttcgt ctattacgcc attcaaaagg agatggaacg
     3541 ccgcagcgcc gaaccgcggt tccaagaatt gctccagagc gcgggcaacg cgctgcgctt
     3601 caccggcaac tgaatgtcgg tcccgtgacc gcccccctcc gcttgggcgc tctgctgacc
     3661 attctgctgg ccacgctggc cgttttgtgg ctggtggccg atgcccttta tgcgcggcac
     3721 attcgcgccg gcgcccaagc gcccaatgcc ccccacgcgc ccgccacgcc gttcttgctg
     3781 aatccgacgg gaacccccgc cctgctgctg atccatggct ttgccgatgg ccccgcggtc
     3841 tatgcgaagc tggcgccacc actggcggaa gccggcttcg cggtgcgcgc cctgcggctg
     3901 ccgggctccg gcgtgccgcc caccggcatg aagggaatca cgctggccga ctggcggcag
     3961 gcgattgacg gcgaaattgc cgacctccgt gccgcggaac ccgcccgtcc cgtctggctg
     4021 gtgggccact cgctgggcgg cgcgctggcc tttgatgccg cgctgcgccc ggctaattcg
     4081 gtggcgggtc tggtgatgat tgcgccactg gtcgaagtct cccgcgcccg ctcgcccgtg
     4141 ctggcccccg aaacatggtt caacctgctg gatcacctgc tgattttcac agacgccatt
     4201 gcctcgcgcc tgcccaaaga cctccacgat cccgacgcgc gcgcgacgta tcaaacggac
     4261 cggtttatcc accgcgatat gtaccgcgcg ctgtttgccg ccaccgatgc aatccgcccg
     4321 cgcgccgccg aatggcacgg tccgctggtc atggccattg ccgccaacga ccaaattgta
     4381 gattcgtccg cctcgcgctt tttctttgcc gccaccaacg ccgcgccctc tgcgctggcg
     4441 gaatatcacg ccgcgggcca tgtgctgccc ttggactacg gccacgacaa actggccgcc
     4501 aaaatcatcc ggttcattca ggaggccccc atgcctgcac ccccgccccc cgttgagctg
     4561 gccacgtttg ccggcggctg cttctggtgc attgaagaaa tcttccgcca gcaaccgggg
     4621 gtgcgccgcg tgacctccgg ctataccggc ggtgaaacaa cgaacccaac ctaccgcgac
     4681 gtctgctccg gcgagaccgg ccatgccgaa gccgtgcaaa tcgaatttga ccctgcgcag
     4741 accagttacg ccgcgctgct ggacttgttt ctgcgcgcgc acgatcccac gcagctcaac
     4801 cgccaagggg cagacgtggg cacgcaatat cggtcggcca ttttcaccca cggccccgcg
     4861 caggcggagg ccgcgcgagc ggcgctggcc gcggccaatg cctccggcca gtttaccggg
     4921 cccatcgtca cccaaatcga gcccgccggg ccgttttatc ccgccgaggc ggatcatcag
     4981 gaatactatt tgcgcaacaa gagcgccccg tattgccgga tggtcatccg gcccaagctc
     5041 aacacgttgg gcctgcaaca gtaggccagc gggagccggc gccccccccc cccccccggg
     5101 gggggccccc ccaaggtggg cgatatttta ggttttgtga aaacggttta tcgtttttat
     5161 tcatttcggg cttgcgctgg gagggggcgc ctgcaaaaga tatgcgcact atgtcgtcac
     5221 gtcgttgcag gattgcggtt ggcgccgatc acggcggctt tgaaatgaag tcgcaactgg
     5281 cggcttggct caaggaacag ggccataatg tgacggacgt gggcacgcat tcaaaggacg
     5341 cggtggacta cccggttttt gcggcggcgg tggcgcgggc ggtggcggcg gggcggtgcg
     5401 atctggggat catggtggat ggcgcgggca ttggatcggc catgacggcc aacaaaatcc
     5461 ccggtgtgcg ggcggcggcg tgctacaacg aggcgctggc gaaaaattcg cggcagcaca
     5521 acggggcgaa tgtgctgacg cttggctcgg gacaaatcaa tttggaacag gcgaaggcca
     5581 ttgcggcgat gtttatcgcc actgactgca ccgaagagag gcacctgcgg cgcgttcaga
     5641 tgatcaagga cctcgaaagc ggccgggcgg gcaccccgat catggcggct ataaaggaga
     5701 tgaaattgga cttatctgca caagacatcg cgcgcatcgc cgagcgcgta cggcaaatcg
     5761 tcggcggggg cggcacagcc agcaccacgg atatgcccaa tctggcgccg gcccaactgg
     5821 cgcggttgat tgaccacacg ctgctgaaac cggaggccac gcaggcggag atcaagcaac
     5881 tgtgcgaggg cgcgctgaag cataatttct acagttgctg cgtgaactcg gtgtgggtgc
     5941 gttatgtggc gcaaatcctg cgcggctcgt ccgtgaaggt gtgcgcggtg gtgggcttcc
     6001 ccctgggcgc ggcgccgccg gaaatcaagg cgctggaggc ccgccgggcc atccgcgaag
     6061 gggccaagga aattgacatg gtgatgaatg tcggcctggc caaggcgggt gactgggacg
     6121 cggtgacgcg cgacatccgc gcggtgtccg aggcctgcaa ggatggcggt gcgctcctga
     6181 aggtgatctt ggaaacctgc ctgctgacca aggacgaaat cgtcaaggcg tgcgaagcct
     6241 ccatgaaggc gcgtgcggac tacgtgaaga cctcgaccgg gttcaacaag ggcggcgcca
     6301 cggcggaaga tattgcgctg atggcccgca ccgtggcgcc gaagcgcctg ggcgtgaaag
     6361 cgtccggcgg cgtgcgctcg tatgccgacg cgatgctgat gatccgcaac ggggccacgc
     6421 gcgtgggctc gtcgaacagt gtaaaaatga tggaagaagc cgcggcggcc tgcggcggaa
     6481 aataaaaagg agctcccatg gctgaattaa ggacctacat attcattgat agcctgcagc
     6541 cgcagtttgc ctcgttcttg gctaccgtgg cgcgcggcta tctgccggtg gctggccaag
     6601 ccgcgctgtt cgtggaaatt tccccgggca tcgccatcaa ccaggtgacc gacgtggcgc
     6661 tgaagtccac gcgcgtgacc cccggcatgc agattgtcga gcgcctctac ggcatgctcg
     6721 aaatccactc cgactcgcag gcggatgtcc gccaggcggg cgcggcgatc ctgaaagacc
     6781 tcgatctttc cgaagaccag cgcatcaagc ccaaggtcta ttcgtcgcaa gtgatccgga
     6841 acattgatga ccaccagacc atgctgatca accgcatgcg ccatgggaac atgattaccc
     6901 gcgggcagtc gctgttcatc atggaagtcg cgccggcggg ctatgccgcg ctggcggcca
     6961 acgaggccga aaaagcggca aacatcaacg tgctggaagt gattacgttc ggcagctttg
     7021 gccggatctg gctgggcggt gaagagcgcg acattgacgt ggcctaccgg gccgcggaag
     7081 ccgcgattga agccgtcagc gggcgggccg aaggcgccgg cgggcgttcc tgattttaga
     7141 aaaaaacaac aacaataaga ggtactaaca tggcagatgc attaggcatg attgaaaccc
     7201 gttccttccc cgccaccgtg gaggccgcgg acgcaatggt caaggccgcc aaggtcgaat
     7261 tggtcgcgta tgagcgcacc ggcggaggct tcacctccgt gatcgtgcgc ggcgacgtcg
     7321 ccgccgtcaa ggcagcgtgc gatgccggcc aaatcgccgc cgggcgcgtc ggcgaagtgg
     7381 tcgctgtcca cgtgattgcc cgcccccacg ccaacgtgga tgcggtgctg cccctcggcc
     7441 gcgtggccac caagccggcg aagtaattcg cgaccggcca acggctggag ccgcccgccc
     7501 cgcgcgggcg gcttccgccc accctttttt gttcgtttgc agttcgtatc gttcccggag
     7561 gcagcatgaa tctcggcaaa gtggtcggca ccgtcgtttc aacccgcaag gagtccgtct
     7621 tggatggcat ccgctttctg ctggtgcggc tggtggatac cgatggcaag gaaacctcct
     7681 cattcgtggt cgcggccgat gccatgggcg ccggccccgg cgaaatggtg ctgacggccg
     7741 ccggttcctc cgcgcgccaa accaccatga cccagaacaa gccggtggac aacgtcatca
     7801 tggcgattgt ggacacctgg gaagtcaacg gcgtcaccaa atacaccaag taaaaccgga
     7861 ttgccgtgcg gcagccactt tcgttttgta acttacggga gaagccatga aacttagcga
     7921 ccaagacatc gaagccattg cccgtcgaat tgccggcgac ctgcgcggct cccccgctcc
     7981 ggccgctgcg gcccccgccg ctgcaccagc gcccgccgcg ttcgccgatg gcgccatggg
     8041 cgtcttccgc actgtggatg aagccgtggc cgccgccaag gccgcgttcc ccgttttttc
     8101 cagtttgtgc ctctccaagc gtggcgaaat tatcgccagt atccgcgcgg tcatgcgcga
     8161 gaatgccacc gcgctggcta aggccgcatt tgacgaaacc ggctacggcc gcttcgagga
     8221 taaaatccag aaaaaccagc ttgttatcga aaaaactccc ggcactgaag atctgctgcc
     8281 ctccgcttgg accggcgacc acgggttgac gctgatggag cccgcgccgt tcggcatcat
     8341 tggcgccatc acccccacca ccaatcccac gtccaccatc atttgcaatg ccatcggcat
     8401 gttggccgcg ggcaacgttg tagtgttcaa tgtgcacccc tccgcccggc aatgctccat
     8461 ccagacggtg gcgctgctca acaaagccat cacggccgcc ggtgggccac ccaacgccgt
     8521 ggtgtgcgtg ggtgaatcat ccatcgaatc cgccgggcaa ctgatgaagc accccggcat
     8581 tcgcgtgctg gtggtcaccg gcggcggcgg cgtggtcaag gccgccatgg ccagcggcaa
     8641 gcgcgccctt tgcgccggcc ccggcaatcc gccggtggtg gtggacgaaa ccgccgatat
     8701 cgaaaaggcg gcctccgaca tcgtctatgg cgcctcgttc gacaacaaca tgatttgcgt
     8761 cgaggaaaaa gaaaccgtgg tggtggcgtc cgtcgcggac aaactcattc aggctatgct
     8821 gcggaataac gcggtgttga ttgacaaatc caaaatcgcg gccatggaac aaactatttt
     8881 caccaagatg gccggcccgc gcggccatgc ggaaatcaac cgcaaatgga ttggccaaaa
     8941 tgcggacaag attctggcgg ccatgggcat ttccgccccg gccaccacgc gcctggccat
     9001 tgtggaattg ccggaagacc acccgctgtt ctggaccgag caaatgatgc ccgtcatgcc
     9061 catttgccgc gtgccgaacg tggactacgc cattgacatc gccctgcaaa tggaaggcca
     9121 caattatcac accgcgctga tgcactcgcg caacattgac aagctctcga agatggcgcg
     9181 ccagtgcaat tgctccattt tcgtcaagaa cggtcgctcg caggcgggcc tcggcttcgg
     9241 cggcgaaggc tacacctcgt tcaccatcgc ctcccccacc ggcgaaggcc tcaccacgcc
     9301 gcgcagcttc tcccgcctgc gccgctgcgc attgattgac cacttccgca tcgtctaacc
     9361 cgtcatgacc gcccgctcaa ctttgtaacc atggattttt cgcccgtcac ccgccccgaa
     9421 tgaaacccca tcccgcattt gccgtcattg aattcaatag cgtgccggtc ggcttgttcg
     9481 ccgccgacgc actgctgaaa aaatcaccga tctccctgat ccgttccggc accatcggcg
     9541 ccggacggtt cctgattctg ctggccggca ccacggcctc cgtggccgaa gcccacgagg
     9601 aagccctctt ccacggcggc ctgcaagtgg ccgatgacgt ctttttgccg gacatccacc
     9661 ccgccctcta tgacggcatt ctgggcaacg tgcgcaacgt gggcgacggc cccgtgctgg
     9721 tgctggaaac cccgacggtc tcctgcaaca ttcaggccgt cgagcgcgcc atgaagggcg
     9781 tgccggtgga tttgctggag ttccgcgcgg gcgacccgcg catggatggc cgcgggttgg
     9841 ccattctgca gggcgatctc tacgacattg aagccgcgca ggaaattgcg ctggccacgc
     9901 tggaaacccg cggcatcccc gcccagcatc gcatcctcac cgcgccgcac gatgccctcc
     9961 ttgcgcaact gtccgtctcc acccgcttcg atttgtccaa gcccctcgcc ctggagggag
    10021 aaaccgcatc ctgacgcgcc gaggtacgcc tatgctgatt ggaaaagtca ttggaaccgt
    10081 ggtctccacc caagtctatg aaggcttggc cggcgtcccc atgctgctgg tgcaaccgct
    10141 gaccaaggaa ggcgtggcca agggcgagcc cattgtgtgc gccgacggca cccgcatggc
    10201 cggccccggc gaaaccgtct attacgaagg cgggcgcgaa gcggccatga ccctcaaccc
    10261 gtggtttgtg ccagtggatc atgccatcgt gggcattgtg gatggggtgg atgcgcccaa
    10321 ctggaaaggc ggcccgacgt gatcatcggc aaagtcatcg gcaacctcca ctccaccatc
    10381 aaccacccgt tttacgacgg taaaaaaatc ctcgtggtgg aacgcattga aacggatggc
    10441 aagcccgccg ccggctatct ggtggcgttg gaccgcgccg gcgccggccc tggcgaaacc
    10501 gtcttgattc tggatgaagg caacggcgcg cggcaggtgt ttgaatctgc cgatggcccc
    10561 gtccgtagcg tcattgtggg catcgtggac gaaatcgcca ccacccccgc ctgaacccac
    10621 ccgcccccta gccccccgcc cgcggccact gtatgcagcg ggttatttct caaatactgc
    10681 ccgaggcaac ccactttgtc ggattatgga ttgcaaagtg ccacgcttga cttcgcggtg
    10741 caacgggatt ggaacagtac gcgtttgggc accgatgtgc ttttgcatta cggcgtgact
    10801 gccgcgttgc cgaacaagaa cgaatccctc ccgctccaac aaccgacaca gtgcttcgcc
    10861 agaaaaaatc ctgaggctag gcataagcag cctcgaagcg agtgacgaac acctcctcac
    10921 gcaggcgttc ttttatctcg gcttcgctgg cacattccag aaaaagctcc acggcttcgc
    10981 gtagattggc cagtgcctct tccaccgaat ctccctgact ggccacatcc aactcagggc
    11041 acaaggcggt atagccactg gcttcttttt ctaaaagagc tgtaaatgtc atgcgcttca
    11101 tgccggtaac ctacgcccgc ttcccttaat ggacaagatg aaaaccggct ggacaggatt
    11161 ggcatacgct gaaagtggca aaaccattcg cgacaaaagc atctggtgct acccaaaaaa
    11221 gttcgagttt gggtgcgccg gggttggaca tttccgggca aatgttggat agttgaaaga
    11281 acgaccatga gcatcggatt cgcccccgcg ggcagaaagt gagcacacca tgaaagccat
    11341 cctcatcatc ctggattccg tcggcatcgg cgccgcgccc gacgcggccg aatacggcga
    11401 tgccggctcc gccaccctcc cccacctggc cgctgccgta ggcggcgtca acctgcccac
    11461 catgcagaaa atgggcttgg gcaacattcc tccgttgctg caccaccgtc cgccattgac
    11521 gattgccggt gtgccgcccg tcgaaaaacc cattgcctcc tatggcgcca tgcgcgagat
    11581 gtccgatggc aaagacacca tcaccggcca ctgggaaatt gccggcctcg aagtggatcc
    11641 cggcttccac aacttcccgt ttgaaaattc tttcccgccc gatttgattg ccgcgtttga
    11701 aaaggaaacc ggccgcaaat tgatcggcaa caaggccgcc agcggtacgg aaatcatcaa
    11761 ggaactcggc gaggaacacc tgcaaaccgg cgcgctgatt tgctacacct cggccgacag
    11821 cgtcttccag attgccgcgc acgaggaaat cgtgccggtt cccgaactct atcgctattg
    11881 tgaaattgcc cgccgcctgt gcgacccgct gcgcgtgggc cgcgtcatcg cgcgcccgtt
    11941 cattgggcag cccggctcat tcaagcgcac ggaaaaccgc aaggattttg cctatcttcc
    12001 cgacgaaaag accattctgg aacgcctgac cgaaaaaggc attccggtct atgccgtcgg
    12061 caaaatcgag gacatctacg cccatcgcgg catcaccgaa tccgtccaca ctggcaacaa
    12121 cgccgactcg caggcgttgg ttgaaaaatg gaccaaggaa aaagataacg gcctgatcat
    12181 cgccaacttc attgactacg acatgctcta cggccaccgc cgcgacgcga agggctacgc
    12241 cgacagcctc gaaaaaaccg accagtggct ggcgaattat ctgcccctgc ttggcgctga
    12301 cgacatgctc atcgtcactg ccgatcacgg caacgacccc acctttcaag gcacggacca
    12361 cacccgcgag tatgtgccgc tgctggtgta tcagcccggc gaaccagtcg agaatctggg
    12421 cgtgcgcaag gggttttacg acattgccca atccctcgcg cgttatttct ccattccggc
    12481 gctgccgcgg ggcgcttcgt tcgtctgaag caactcccgt cgaatggccg gtcgcgtttc
    12541 ccgcggccgg ctctttttgt gaccgcgcgg cggcgcttat tcagacgctt ccgccagttg
    12601 gcgaatgcgg ctcatcacga gcgcgccaac ggcttcgtag gcgcccttgc cgggcatggc
    12661 ggcaaggatt tcatccggct ggatggccgg gcccaagcgc gcggtaatgc gggcaggccg
    12721 gaacttgctg gcaccttttg ggaaggctgc aaacgtgccg gagatataca gcggcaccac
    12781 cggcaccgca gccttggcaa tcaaaaatcc aatgccaccc ttggcctcgc gcatttggcc
    12841 atcgggcgag cgggtgccct cgggaaaaag gccaatcacc cggttttcct tgagcatggc
    12901 cagcgccttt ttcagcgcgc ccagatcgcc gcgggtgcgg tccaacggaa tcacccccac
    12961 gcgcggaaaa aaccagcgcg ccatggcgtt ggaatacagc gtatcgcgcg ccatgaaatg
    13021 cacaatgcgc cgcgcgctgc aggccgccat caccggcggg tcgagatagc tgcaatggtt
    13081 ggcggcaatc accgccccgc ctttggccgg cacatactgc cgcccagtca cccgtagccg
    13141 aaatttgatg cgcagcgcca gggccacgaa atcccgagca acccagaaca agggcttttg
    13201 aaagacccct tcgcgcatgg aatcaggagc cgaccacgcc ggcctgcttg aggtcggtca
    13261 caatcgtgtc caccacctgc tcgatggtca tgccggtgct attgatgatt ttggcgccca
    13321 gcgcaatttg cagcggcgcg gtggcgcggc tggaatcctt ctggtcacgg cgcttcagcg
    13381 aagacagcac ggcagccacg tcatcggtgc cctccgcctg cttgatttcc gccgcgcggc
    13441 gacgggcgcg ctcttcggga tccgcatcga ggtaatattt gaaaggcgta tcaggaaaca
    13501 ccacggtgcc gatgtcgcgc ccttccatca cgagattgcc aaacttggtc atcaggcgca
    13561 gtttggccac aatatacacc cgcaccacct gctgcgcggc gatgtccgcc acgcgctcgc
    13621 ggacgggctc cgcgcggatt tcctggccgg ggtcctcgcc atcaatcagg tagcctacgg
    13681 cgttgtcgcg caggaccagt tccatgttca tccggatcag caggtcggtc acggcctgcg
    13741 tgtccttgac atcaatgcct tccttcacgc acttccacgt gatgccacgg tacagcgcgc
    13801 cggaatccac ataaatgaac cccacggcct gcgccgtgcg ccgcgagacg gttgatttcc
    13861 cggacgccga ggggccgtcc actgccacaa tgatgctttg cttattcaac tttcactccc
    13921 agtttcgcga gttgctccca aaattccgga tacgacgtat ccacacaagc cacattggca
    13981 atcgtgacgg gctgatccgc aaaaatggcc aggatggaca tgctcatggc gatgcggtga
    14041 tcgccatagc tgcggacgct cttgatgggc gagaccttgg tggggccttc gatgatcatc
    14101 ccatcttcct gctcggtggc atgcgcgccg aggttggtca gattttcgac catcaccgcc
    14161 acgcggtcgg actccttcac acggagttcg ccggcatcgc ggatgacggt gcgccccttg
    14221 gccaacgcgc ccagcacggc caccaacggc agctcgtcaa tcaggttggg gatttccgcg
    14281 ccgccgacct cggtgccctt gagttccgcg ccatacaccg tgacattgcc atacacttcc
    14341 ggatcggtcg gcgccgaggt acgctcgatt tccacccgcg cgcccatgcg ccgcagcaca
    14401 tccagcagcg cggtgcgccg caggttcagg ccgatgccct ccgcgcgcac ggtaatcccc
    14461 ggaatggccg ccgccgctgc cagccagtag gccgccgacg aaaaatcacc cggcaccacg
    14521 taggggcgcg ccagaaactt gggccctttc ggcccgtagc catccacgga aatccgctgg
    14581 ccttccacgc gaatcggcgc gcccaccgcg cgcagcatcc gctccgtatg gtcgcgggtg
    14641 gggatgggtt ccaccaccgt ggtgctgccg ttggcataca gccccgccaa aatgcagcaa
    14701 ctcttgacct gcgcggaagc cgtcggcagg cagtattcaa taccgtgcag gttgccgcca
    14761 tggatcgtca tcggcgcggt gccttgcggc gtcagctcca ccgtggcgcc catgagttcc
    14821 agcggctcct tgatgcgccg catgggccgc ccgctcagcg aggcatcccc cgtgagcgtc
    14881 acggtaatcg gcgagccggc gcacagcccg gccagcaacc gcattccggt gcccgagttg
    14941 cccacatcca acggcccgac gggctcaatc atcttgcccc cggtgccctg aatgaccagc
    15001 acaccctctt catccaccca cgaacgcgcc ccgaaggatt ccatcgcctt gagggtgttc
    15061 aggcaatcct ccgcctgtaa aaatccacga atttccgatg tgcccgacga gatgcctgcc
    15121 agcagcgccg tgcggtggga aatgctcttg tcgcccggca cccgcaccac cccgccgtcg
    15181 aagccgccgg gataaacgat tttggtttgt tttggaatgg ccatgttttg ttgtgtagtt
    15241 gtctggtttt tatgcaggcg cggcttaccg gccacgcccc tttaatagtt cggcgcgggc
    15301 cacgcgcgcc cgctccaaaa aagccgccac gtcgccaaag cgaccggctt gaagatcatc
    15361 atatacctgc tcgacctcga ttttgaaagc ccgcaattcc tcggccaccg ccgcggcgtt
    15421 ggtcagcaaa atatcgtgcc acatgtcaat ggaccctgac gccacgcggg tggagtcata
    15481 gaagccggtg ccacagaaag cgcccacctg ctccgcgcag tcgcggccga tggccttggc
    15541 cagcgcggcg gcggccacgt gcggcaggtg gctggtccgc gccagcaacc ggtcgtgttt
    15601 atcgggctcc atccggcacg tacgcgcgcc cgcggcctgc cacagcgccg ccacgcgcgc
    15661 caccgccggc tccggcgcat ccaaatggct gcacagcacc gtcagcgcgt tggcatacaa
    15721 atccgccgat gccgcctgca aaccctgctt ctccgacccc gcgatggggt ggcttcccac
    15781 aaacgtcccc ggcggaagca gcccggccat ctgacggcaa atccagcttt tggtgctgcc
    15841 cacatccgtc acgagcatcc ccggctgcag cagcggcgcc agttcgccgg caaactccac
    15901 gcaggagcga atcggcacgc acagcacgat cagcgtcgtg cccgcgatgg ccgcatcggg
    15961 atcggcgtac gccgcatcca caatgccgcg cgccacggcc tcctgccggg tttcctcgcg
    16021 ccgcgcgtac gccaccagcc gccccgcata cccccgcttg cgcagcgcca tcgccagcga
    16081 tgcccccatc aatcccagcc ccacaatcga aatgcccgct tcctgcgtca tgcgcccctt
    16141 aatgcaaatt acgccccacg gcccgcgcca ccaactccaa cgcccccagc agttccttga
    16201 accgtttggg ggtcaacgat tgcgccccat ccgacaccgc ctgcgccgga tccggatgca
    16261 cctcaataat cagcccatcc gcgcctgccg ccaccgccgc cttggccatg ggcgccacca
    16321 aatcccactg ccccacgccg tggctggggt ccaccaccac cggcaggtgg ctctcgcgct
    16381 tcagcaccgg taccgcgctc agatccagcg tattgcgcgt gaacttttcg aacgtgcgga
    16441 taccgcgctc gcacaggatt acctccggat tgccggcgct caccacatac tccgcgctca
    16501 tcagcagctc ttccagcgta ttcgccagcc cgcgcttgag catcaccggc ttgccgcagc
    16561 gccccaccgc cttcagcaaa ttgaagttct gcatgttgcg cgcccccacc tggataatat
    16621 ccgcaaactc gcccaccatt tccacatcgc gcgggtccat gacctcggta atcaccggca
    16681 gccccaccac cgcccgttcc tcggccagat agcgcaaccc ctccaccccc agcccttgga
    16741 acgcataggg cgacgtccgc ggcttgaacg ccccgccccg cagcaggtgc gcccccgccc
    16801 ccttcaggaa cgcccccagc gataaaatca tctcgcggct ctccaccgca cacggccccg
    16861 ccatcaccac cacccccgtc ccgccgatgc tcaacccccg gtccccgtgc cgcgccggcg
    16921 ggattttgat gacggttttc tccggatgga actccgtgct gacccgcttg tatggcttca
    16981 gcaccggcag caccttgtcc acgcccggca tcagggccaa cgtcgcctcg tgatcgcgcg
    17041 ccgcgtcgcc gatcaagcca atcacggtca cttccgtgcc ggggctcaca tgcgccttta
    17101 gcccccactc ggccacggct gccgtcagcg cttcaacctc ggcctccgtg gccccttttt
    17161 tcattacgac aatcatacac cactcaataa aatcctcttg ggcgccgcct gccggccgcc
    17221 cagtatttgg aagcccaaca ctactccccc aacggcccct tgaccaaccc aaaaaacacc
    17281 cccctcgcca atacttttcc acaccgtgga aaaatcagcc caactttttc cacagtgtgg
    17341 aaaaatcgcc tcaaagttgt tccattgtgt ggaaaagtgg ggtcaaagtt gttccattgc
    17401 gtggaaaaaa cagggcg
//
LOCUS       DAOPNP010000047        17192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_3967_length_17192_cov_8.952325, whole genome shotgun sequence.
ACCESSION   DAOPNP010000047 DAOPNP010000000
VERSION     DAOPNP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_17192_cov_8.952325"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKI64_05810"
     CDS             <1..849
                     /locus_tag="PKI64_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362699.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOE00561.1"
                     /translation="KLALNDSSFHYQTESSVALGFGFRCGFLGLLHMEIVQERLRREY
                     DCDIIATYPGVVYRVRMNDGAVREVDNPVFLPDPTEFEAIEEPIVKLYLICLGEQIGD
                     MMKLVMDKRGLIERTESLDTKRVMLTCIIPLNEILIDFHDRLKSISRGYASMDYEPAG
                     YREDDLVKLDILVHGEPVEAFSCIVHRDKAEARGRQICEVLVDLIPPQQFAIAIQAAL
                     GAKIIARTTVRPYRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKQFGKVGIPQEAFIA
                     VLKSKQ"
     gene            858..1976
                     /gene="lepB"
                     /locus_tag="PKI64_05815"
     CDS             858..1976
                     /gene="lepB"
                     /locus_tag="PKI64_05815"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOE00562.1"
                     /translation="MNFLARRRWKKNVQQAQHVVRHALNMREDIAPAEHVAAARAADG
                     RLEQCWAARDWAAMEAACEEALAAAEQLLPPHPFPKWRENVEVLVVAIALAMACRTYF
                     IQPFKIPTGSMQPTLNGVTATPQDGQRWYDRLPFNLVGLALFGERYVEVRARNSGRLE
                     YAGIVNDQFTYRIGLELYPLRLAMLPDSAQVDPARSMKLHAQPGERVAKGQLIASGRV
                     RQGDHIFVNKVKYNYQRPRRGDIVVFDTNYIPGKERLGIVPNSFYIKRLAGLPGETIA
                     IRGRHLVADGVEITAPEPFQRLVQDPQYHGYVTRPGALLNAAESELPVGEKQFLPFGD
                     NTYSSLDGRYFGPVTEQALVGPSFFVYWPLGAHWGPTR"
     gene            complement(2004..2435)
                     /locus_tag="PKI64_05820"
     CDS             complement(2004..2435)
                     /locus_tag="PKI64_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOE00563.1"
                     /translation="MTMPAWFFWALLSAVFAAGTAIFAKIGIRGVDSDLATLVRTFII
                     FFVLMAYVAALGKWSNPLHLPGKTWLFLTLSALATGASWVCYFRALQIGEASKVAPVD
                     KFSVVLVALFAFVFLGERPSLREWSGIACVALGVVILALKK"
     gene            complement(2450..3256)
                     /locus_tag="PKI64_05825"
     CDS             complement(2450..3256)
                     /locus_tag="PKI64_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00564.1"
                     /translation="MRTIRIHLDAHAHIYPFYDVPRLLVAALDHMPRHTPTDLRVLAL
                     AERAECSFFQALAQDEIQLPGDRWRIIAWDPDGGVKVRHLPDARELWIIAGRQFVTAE
                     RLELCALGSDAPHADGQPAVELARAILTAGGLPALNWAPGKWLFARGKTVKALLQTFS
                     PTQLVLVDSSLRPFGWPAPAAFRSARRAGRAVLAGSDPLPFGGEEIRAGSYHCTFTMP
                     ALADAARLVAELKTALANGPKDLRWQGRRGTPATVWQRLRRNAKVPPPAT"
     gene            complement(3253..4989)
                     /locus_tag="PKI64_05830"
     CDS             complement(3253..4989)
                     /locus_tag="PKI64_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOE00565.1"
                     /translation="MNFRLTGFLALLAAFLGALILLWDGDENTTGARLEQARRAFRFD
                     PARVDRLLLEAGELTIECRRAEREWRLVRPLEARADPIALERLLGALQELPRGDILLP
                     PPRPEGTFAPFGLEPPRATLSLVSGVVTNRILIGRRTPLGDGIYVRQADQPALARLNL
                     ALLDLLPTSADALRDRSLLAGAPADIVRVDIRGPAGYVQLARDEAGAWRIYQPFTARA
                     AAPLVTAIIEKMLACSIVQFVQDTVSDLAPYGLDSQSAVTVVFNTDSGNGSQMLALGD
                     PLPNDPSLVYARLQGENSIYAVPADARQSLLVHPEDLRDRRLPGLDADTIATLRVETA
                     DAALELQQDATDTWQIITPLRVAANPMAIQDLLRSWADVRLDPAPDAPTNLPPWSRLI
                     RITPRSPKAAPLEIHVAPIPDDPTACWLAIEGESAISRATPAAPLTSPLDPLRYHSLD
                     IASVPLDDIERIRIATPTQGITVERDAATGQWSPPTPWVDRLLAVLTPLRAESIVPAT
                     EAPGDAVDAIHPFLLLTVQQRGQSGLATTLTVGRQATPDGARYATLRGRDFLFILPAW
                     TIEALLPPAENP"
     gene            complement(4992..6449)
                     /locus_tag="PKI64_05835"
     CDS             complement(4992..6449)
                     /locus_tag="PKI64_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOE00566.1"
                     /translation="MSLLPSSPLAASRLQRIVLRANVALAVLLSVAIFAMVNYLAQRH
                     YARWHWRAAHSVRLADASRRLLEATANEIRLHVLVRPGHEAYGPLALLLQEYAAASGN
                     VNVEFVDPDLAGARTKELARQFNLSGEECVVLATGGRHLAIPAADLVEYVLPANTTEN
                     PRRLFRGEALISGAIHALTQATRPVVCFLEGHGEHAPVDFDRLRGYSRLAALLRDQNL
                     DLEQLDLGQTKMVPARCALLVVAGPQRELAPFEVALLRDYLTRKGRLLLLLDARVSTG
                     LEALLHDWGIALGDDVVVDEAHTLSGRELYVASYPPHAITAPLQDLASVFVLPRSVRA
                     RTPEAGGDQPIVTPLALCSSAGWAEFNLDDAAVHFDPQVDIPGPVPVAVAVERGPVAG
                     VHVQIRPTRLVVFGDSDFVSNGGLTGANADFFLGAVNWLLDRDIAMAITAPALPELQL
                     VMNARQLRQLFWLAVVALPGAVMLVGLFAIWRRRR"
     gene            complement(6456..7202)
                     /locus_tag="PKI64_05840"
     CDS             complement(6456..7202)
                     /locus_tag="PKI64_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE00567.1"
                     /translation="MSAFWTLWFRELAAYFRTGIGYVIGTTFLAIAGISLWVLATNLA
                     QGPLAGDLAGNLFGSTGYWLAILLVTPLLTMRLFAEERRMGTLPTLLVAPIGGTTVVL
                     AKFAAAYTMFIALWLPTLVFAVLLRRCGAHLPPLDWGPVAAGYLGTALVGALFLAIGL
                     LCSLLTRHQAIAAMACLGVLSLLLTGGLLPLHAPLAAIRQLAHQLAAPRHMADFAVGI
                     VDTRVILWYASATVLLLFTAVRLLEVRRLR"
     gene            complement(7199..7969)
                     /locus_tag="PKI64_05845"
     CDS             complement(7199..7969)
                     /locus_tag="PKI64_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00568.1"
                     /translation="MAATELAIEVDGLTRRFPRCEALGGVSFQVPRGELTGILGPNAS
                     GKTTLLLILTGLLVPTTGRVRVAGFDPTTQWLEVRRRIGFLPENVPLYPEMRVQEYLA
                     FRGRLKGLRGKRLAERIRQVLEPCGLGEMARRPIGTLSRGFRQRVGMADCLLTEPEIL
                     LLDEPLAGLDAGQTQRFHEMLREQCGHRTILFSTHVLAEAEQLCQRVLVLNGGRLAAN
                     DTPAQLAAATPTGSFTDAFLRLTTLPPRVQNPRGRSAR"
     assembly_gap    8262..8361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8362..9330
                     /locus_tag="PKI64_05850"
     CDS             <8362..9330
                     /locus_tag="PKI64_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE00569.1"
                     /translation="ARRVWERDRSALVVQVVELFETLIYLRHQVTCDEEFAERMSGLR
                     QLAAARAEQGKASRTDVLRMEWQEGEAQLRLETGRAQLAIRFQELANVLGLPLTTSFT
                     LVPPPLLDLTTGETDEALAVALAERPDYAQALQDVETSSRQERLARRRLLPNLRLAAT
                     QTTYGEGPEWSDATRLDQEDWFVGLTADMNLNLRGTRLDVARAGVDVAVREHVAEIVR
                     NRLAVEVHAALAEYRRSRAALALAARNRELAADRAELARTLFTAGRAPADMVSDAETD
                     SSSAMLRELAARREAAVAAYRLLHVLGTLVPAPKELLRPTSQGTES"
     gene            9327..10529
                     /locus_tag="PKI64_05855"
     CDS             9327..10529
                     /locus_tag="PKI64_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOE00570.1"
                     /translation="MKNVAGWRGWCGVALLLLAGCGRPAAPAPRVAVVTPGAIRMTVP
                     FAGELAARRVEMITAGMQGSAVLAELVPEGTKVAAGELLARFDTSQFAQDLARQEHEL
                     IRARQELDSLAKAELPLELLDMETRQQDGLAEVAAEERFLESARDLAARGLMSADEVA
                     RQEQKVAGLRQRNEQWDTRIALTRDHVHAARLAKAQAAVEAAERQRDFTAQQVALCEV
                     RAPVAGIVTLVPLPIGGEYRPAHVGDTLFRNQAFMCLPDPAEHVLRGYVDEAELPWVQ
                     PGNRVEATPVAYPHARLTGRVEMVGGMAQTRPGDPAWRKRFPVDIALDPLPEPMPVGI
                     SVRADIVAGENTHALLLPREAVAWHHLQAVVRRPNGDEIPVQTGLADATHVEIVAGLA
                     AGGEVLLP"
     gene            10532..11674
                     /locus_tag="PKI64_05860"
     CDS             10532..11674
                     /locus_tag="PKI64_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00571.1"
                     /translation="MFWADIWTGLRTGPARALLALVSLALGLFAMTILLATLDALQRQ
                     ADRLVQAFGAGAIVLRPEAGQAPWGRAMVDYFRANLGADAAVSGVRTLPPPAGADFAV
                     AAGDDQLARARGWRIVAGRALDGWDLRHAARVALAPAGLCQRKNWREGDLVLLGHEPF
                     RLVGCYEAGGEPVPGFAPDTVFIPATVAALGAGPRDERGDISALLLTAAGTTAPEDLQ
                     RRARGLLRGFGLDDNAVEWITPDTLLRGVRRWQQAVGWVAGSGAALGLLLGAVSLAGM
                     LLTGVRERVAEIGLRRALGARPHEIALLFVAEAAVLGLGAALLGLLGAGAVLLGQGAR
                     FPLPFHFGVGAWLIPLVLAGGIVILCSLGPAWQAARLPPAEALRNE"
     gene            11667..13205
                     /gene="amrB"
                     /locus_tag="PKI64_05865"
     CDS             11667..13205
                     /gene="amrB"
                     /locus_tag="PKI64_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE00572.1"
                     /translation="MNKREWLLLLVLLATSACAREGELEMSKTCMGSSLAGAWYEANP
                     ERLRAELQGYLDAAEVTPDPTIFAVIAPHAGYAYSGPCAAVAIKALAARTNLSRVVVV
                     GFAHRVRLAGRASVPSQETCYRSPLGDTPLDTATIAGLLKKTVFVDKAATREGENSVE
                     MMLPLLQVALTGREWQLLPITLGQLDERGLAEVADALAPLLDGQTGFVFSSDFTHYGP
                     NYGYVPFRTNIAGNLRKLDSGAVEKIMAGDAAGFAAYCQETGATICGQDSIGVLLRML
                     PDNFTARELFYDTSGRRTGDFMNSVSYEAIGFYGEGPTIHRPAPAVAESAAADPLSAA
                     DKQALLAMARQAIARTFDGQPPADAKALGAELSPAMKKKMGGFVTLTIGGELRGCIGE
                     IFPRRALADVVVDHAQDAAFHDPRFPPLTKKEFAQVKIEISALTPPEPVASYRDIVIG
                     RHGMVLELRGRSAVFLPQVAPEQGWDLATTLTHLAYKAGLPGNAWQDPKATFTVFEAV
                     VFHE"
     gene            13400..14344
                     /locus_tag="PKI64_05870"
     CDS             13400..14344
                     /locus_tag="PKI64_05870"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00573.1"
                     /translation="MSRRQIAKKSGILGALLCALLPLGAGAQNTETKLTPPLYIGNTQ
                     PVRDTFGRVMRGSPNHREGRALVEVRVATNRIVRPPYKDGSAHPYHPLLNGAEGAGGI
                     GQNCANDNTGLFCLAFHKRPAPGTVVFARVFNAPTAQEATFYADSEPVEITALDTAVV
                     VEFGALKPLDADDDDNDGLNNSWEKLLGTDDRLTADYDGDGMSDLHEMLAGTAPDDPN
                     SRLAFHTVHRERTARVVKAGDPPVRPIHVSWQSVPGKRYQLEYVAQLVPDPVTGEEHE
                     IIPIGNVITAGENEYEIEKCVDLPDDAVTGTFRIRLVR"
     gene            14431..16233
                     /gene="mutL"
                     /locus_tag="PKI64_05875"
     CDS             14431..16233
                     /gene="mutL"
                     /locus_tag="PKI64_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOE00574.1"
                     /translation="MDTPAQIRVLPDTVINQIAAGEVVERPASVLKELVENSLDAGAR
                     RIEVDVVDGGRRLVRVRDDGAGMDHDNALLALERHATSKLRALQDLDHIATMGFRGEA
                     LAAIASVAQFTLLTNRAGSATGTEILVFGGRIQEVRDAGVPPGTDIAVRNLFFNVPAR
                     RKFLRSAPTEFTHIRQAFIHEALANPSVEFILRADDADVYRLPAGTLLDRIRDLYGAE
                     MAAHLRPLNFGNGSVEISGYAGVPPFSRLDREWQIVFINERPTSSPVVSFAIQSAYRD
                     VLVGGRYAPLFMQVRVPSDAVDVNVHPAKKEVRFHRPTEVRDVIIEAIRLAISGGEMP
                     KGKRSAAAPHRAEPVAPAPAAEPPPAVVAPAAVSPAAPPPAPVVPAPVATIQQQWTWA
                     SSQPSATPDGLPARVTPWAEYRLLGRVAGLYIVLETPDGLVLMDTRSAHERVIYERLL
                     HAVDQGRAPSQGLLPPVAVPLSPAQTNALRKHLAALKQMGFGVAEFGAETFMVDALPV
                     WVAHAAPGPLLSDIADGLMQGGRATAKDWVLPLIAETASRQAVSLQRELNEAELNALI
                     RDLAGTEMPYTSPRGKPTVILLSHRELNRKFGRE"
     gene            complement(16241..16783)
                     /locus_tag="PKI64_05880"
     CDS             complement(16241..16783)
                     /locus_tag="PKI64_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOE00575.1"
                     /translation="MTATLLHGLAAAGVFLAWAVCALLCATGLLLAMFSISGTWLVLA
                     AGIVAALLTGSAEFPGWPTLLGMLVVCVAVDTGEWFAVRWGVQRRGGSRAAGWLALLG
                     SVGGAVAGGLVVPIIGNLIGMLLGCFLPVFFYEQHRLKKADGAVRIANGAVLAGLIVL
                     LQKVAATTGMVLWLAAGLLF"
     gene            complement(16786..>17192)
                     /locus_tag="PKI64_05885"
     CDS             complement(16786..>17192)
                     /locus_tag="PKI64_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE00576.1"
                     /translation="ILAYLGGIATHIWMIKGYFDSLPVALEESAAMDGASPWQIFLRI
                     MLPTSLPIFAVVFILAFIGLLGEYPLASVVLQRTDQWTLAVGANSFLYEQNYRWGDFA
                     ATAVLAGLPITLLFLACQKLLVSGLTAGGVKE"
BASE COUNT         3153 a         5138 c         5917 g         2884 t
ORIGIN      
        1 aagctggcct tgaacgacag ctctttccac tatcaaaccg agtccagcgt ggcgctgggt
       61 tttgggttcc gttgcgggtt tttggggctt ttgcacatgg aaatcgtgca ggagcgcctg
      121 cgccgggaat acgactgcga catcatcgcc acctatccgg gcgtggtgta ccgcgtgcgc
      181 atgaatgacg gcgcggtgcg cgaagtggac aacccggtat tcctgccgga tcccaccgag
      241 tttgaggcca ttgaagaacc catcgtcaaa ttgtatctga tttgcctcgg ggaacaaatt
      301 ggcgacatga tgaagctggt gatggacaag cgcggactga ttgagcgcac ggaaagcctc
      361 gataccaagc gcgtgatgtt gacgtgcatc attcccctca acgaaatcct gattgatttc
      421 cacgaccggc tgaagagtat ttcgcgcggc tatgcctcga tggattatga gccggccggt
      481 taccgtgaag acgatttggt gaagctggat attctggtgc acggagagcc ggtggaggcg
      541 ttttcctgca ttgtgcaccg cgacaaggcc gaagcgcgcg ggcggcagat ttgcgaggtg
      601 ctggtggatt tgatcccgcc gcaacagttt gccattgcga tccaagccgc gctgggcgcc
      661 aagatcatcg cgcgcacgac agtgcggcct taccgcaagg acgtgaccgc caagtgctac
      721 ggcggcgaca tcacgcgcaa gcgcaagctg ctggaaaagc aaaaagaagg caaaaagcgc
      781 atgaagcaat ttggcaaggt gggcattccg caagaggcct ttattgcggt gctcaagagc
      841 aagcaatagg ggcgctcatg aattttctgg cccggcggcg atggaaaaaa aacgtgcagc
      901 aggcgcagca tgtggtgcgc catgcgctga acatgcgcga ggacatcgcc cccgcggagc
      961 acgtggcggc ggcccgcgcg gcggatggcc ggttggagca atgctgggcg gcgcgggatt
     1021 gggccgcaat ggaagccgca tgcgaagagg cgctggccgc cgcggaacaa ctgctgccgc
     1081 cgcatccgtt cccgaaatgg cgcgaaaacg tggaagttct tgtggtggcc attgcgttgg
     1141 cgatggcctg ccgcacgtat ttcatccagc cgttcaaaat ccccacgggc tccatgcagc
     1201 ccacgctcaa cggggtgacc gccacgccgc aggatggcca gcggtggtac gaccggctgc
     1261 cgttcaatct ggtggggttg gccctgtttg gcgagcgcta tgtggaggtc cgcgcgcgca
     1321 attccgggcg gctggaatat gccggaatag tgaacgacca attcacctac cgcatcgggt
     1381 tggagctgta cccgttgcga ttggccatgc tgcccgacag cgcccaagtg gatccggcgc
     1441 gcagcatgaa actccacgcg cagccgggcg aacgcgtggc caaaggccag ctgatcgcgt
     1501 ccggccgcgt acggcagggc gaccatattt ttgtcaacaa ggtcaaatac aactaccagc
     1561 gtccgcggcg cggggacatt gtggtttttg acaccaacta tataccgggc aaagagcggc
     1621 tgggcattgt gcccaacagt ttttacatca agcgattggc gggactgccc ggcgaaacca
     1681 ttgccatccg cgggcggcac ttggtggcgg atggcgtgga gattaccgcg ccggaaccct
     1741 tccagcggtt ggtgcaggat ccgcaatatc acggttatgt cacgcggccc ggcgcgctgc
     1801 tcaacgcggc ggaatccgag ctgccggtgg gcgaaaagca atttttgccg tttggcgaca
     1861 acacctattc cagcttggac ggccggtatt ttggcccggt gacagagcag gcgttagtcg
     1921 ggccatcctt ctttgtgtat tggccactgg gcgcgcattg gggccccacg cgctgaagcg
     1981 tgtgtttttg ggcgtgcggg gggctacttt ttgagcgcca gaatcaccac gcccagcgcc
     2041 acgcaggcaa tgcctgacca ttcgcgcagg ctgggcctct cgcccaaaaa tacaaacgca
     2101 aacaaagcca ccagcaccac gctgaactta tccaccggcg cgaccttgga agcctcgccg
     2161 atttgtagcg cacggaaata gcacacccac gacgcgccgg tggccagcgc ggagagcgtc
     2221 aaaaaaagcc atgttttgcc gggcaggtgc agcgggttgc tccatttgcc caacgcggcc
     2281 acgtaggcca tcagcacaaa gaaaatgata aatgtccgca ccagggtggc cagatcggaa
     2341 tccactccgc ggatgccgat tttggcaaag atggcggtgc cggcggcgaa tacggccgag
     2401 agcagggccc agaaaaacca agcgggcatg gtcatggcgg atttaccttt catgttgcgg
     2461 gcgggggaac tttggcgttg cggcgcaggc gttgccaaac ggtggcggga gtgccacggc
     2521 ggccttgcca acgcaagtct tttgggccgt tggcgagcgc ggttttgagt tcggccacca
     2581 agcgggcggc atcggccagc gcgggcatgg taaacgtgca atggtaggag ccggcgcgaa
     2641 tttcttcgcc gccgaagggt agcggatcgg agccggccaa cacggcgcgg cccgcccggc
     2701 gcgcagaacg gaaggctgcg ggcgcgggcc agccaaatgg ccgcagcgag gaatccacca
     2761 gcaccagttg cgtcggcgaa aaagtctgca gcaaggcctt cacggttttg ccgcgcgcga
     2821 acaaccattt gcccggtgcc cagttgagtg cggggagacc gccggccgtc aaaatagcgc
     2881 gggcgagctc cacggctggc tggccgtcgg catggggggc atcgctaccc aacgcgcaca
     2941 gttcgaggcg ctcggccgtc acaaattgac ggcccgcaat aatccaaagc tcgcgggcat
     3001 cggggaggtg gcgtactttg acgccgccat ccgggtccca cgcgatgatg cgccagcggt
     3061 cgccgggcaa ctggatttca tcctgagcca acgcttgaaa aaaagagcat tccgcccgtt
     3121 cggccaacgc gagcacgcgc agatcggtgg gggtgtggcg gggcatgtga tcgagcgcgg
     3181 ccacgagcag gcgcggcaca tcataaaaag gatagatgtg cgcgtgggcg tccagatgga
     3241 tgcggatggt tctcatgggt tttcagcggg cggcagaagc gcctcgatgg tccaagcggg
     3301 caaaataaaa agaaaatcac gtccgcgcag ggtggcatag cgcgcgccgt cgggcgtggc
     3361 ctgtcggccc acggtaagcg tggtggccaa gccgctttgg ccgcgctgct ggaccgtcaa
     3421 caacaggaag gggtgaatgg cgtccacggc gtcgccaggc gcttcggtgg cgggcacgat
     3481 gctttcggcg cgcagcgggg tcaacaccgc cagcaaccgg tccacccagg gggttggggg
     3541 gctccattgg ccggtggcgg catcgcgctc caccgtgatg ccctgcgtgg gcgtggcgat
     3601 gcggatgcgc tcgatatcgt ccaacggaac ggaggcgata tccagcgagt gatagcgcag
     3661 cggatccagc gggctggtca gtggggcggc gggggtggca cggctgatgg cggattcgcc
     3721 ttcaatggcc agccagcagg ccgtgggatc gtcggggatt ggagccacat gaatttcgag
     3781 gggcgcggcc ttgggcgagc gcggcgtaat gcggatcaaa cgtgaccacg gcggcagatt
     3841 ggtgggggca tcgggcgcgg gatccaaacg gacatcggcc cacgagcgca gcaagtcctg
     3901 aatcgccatg ggattggcgg ccacgcgcag cggcgtgatg atttgccacg tgtcggttgc
     3961 gtcttgttgc agttcgagcg cggcatcagc ggtttccacg cgcagggtgg cgatggtgtc
     4021 ggcatcgagt ccgggcaggc ggcggtcgcg caaatcttcc ggatgaacga gcaaactttg
     4081 gcgcgcgtcg gcagggacgg cataaattga attttcgccc tgcaggcggg catagaccag
     4141 cgacgggtcg ttggggagag gatcgcccag ggcgagcatt tgcgagccat tgccggaatc
     4201 agtattgaac accacggtca cggcgctctg gctatcgagg ccgtagggag ccagatcgct
     4261 gacggtgtcc tgcacaaact gcacgatgct gcaggccagc attttttcga tgatggcggt
     4321 gacgagcggc gccgcagcgc gcgccgtgaa cggctgatag atgcgccaag cgccggcttc
     4381 atcgcgcgcc agttgcacat agccggcggg gccgcgaata tccacccgca cgatgtcggc
     4441 gggcgcgccg gccagcagtg agcggtcgcg gagggcatcg gcgctggtgg gcagcaaatc
     4501 cagcagcgcc agattcaacc gggcgagggc gggttggtcg gcctgccgca catagatgcc
     4561 atcgcccagc ggggttcgcc gaccaatcag gatgcggttg gtgaccacgc cggacaccag
     4621 ggaaagagtg gcgcgtggcg gttcgaggcc gaagggcgca aaggttcctt ctgggcgcgg
     4681 cggcggcaac aaaatatcac cgcggggcag ctcttgcagt gcgccgagca gacgttccag
     4741 cgcgatgggg tccgcgcgcg cttccagtgg acggacgagc cgccactcac gttcggcgcg
     4801 gcggcactcg atggtgagct cgccggcctc cagcagcagg cggtccacgc gggcgggatc
     4861 aaagcggaac gcgcggcgcg cttgttcaag gcgggcgccg gtggtgtttt catcgccatc
     4921 ccacagcagg atcagcgcgc cgagaaacgc ggccagcagc gccagaaaac cggtcagccg
     4981 aaagttcatg gtcagcgcct ccgccgccag atggcgaaca agcccaccag catcaccgcg
     5041 ccgggcagcg ccaccacagc cagccaaaac agttggcgca actggcgggc attcatgacc
     5101 agttgcagct ccgggagggc gggggcggta atggccatgg cgatatcgcg gtccagcagc
     5161 caattcaccg cgccgaggaa aaagtcggca ttcgcgccgg tgaggccgcc gttgctgaca
     5221 aaatcggaat cgccgaacac cacgaggcgg gtggggcgga tttgtacgtg aacgccggcc
     5281 accgggccgc gctcgacagc tacggccacg ggcaccggac cggggatatc cacctgcgga
     5341 tcaaaatgta cggcggcatc gtcgagattg aattctgccc agccggcgga ggagcacagc
     5401 gcgagggggg tcacgatcgg ctgatcgccc ccagcttcgg gggtgcgggc gcgcacggag
     5461 cgcgggagaa caaaaacgct ggccaaatct tgcagcggcg cagtgatggc gtgcggcggg
     5521 taggaggcca cataaagctc gcgcccgctc agggtgtgcg cttcatccac cacaacatcg
     5581 tcgccgagcg cgatgcccca atcgtgcagc aaggcttcca ggccggtgga cacgcgggca
     5641 tccagcagca ggagcaggcg gcctttgcgg gtgagataat cacgtagcag ggccacttca
     5701 aagggggcaa gctcgcgttg cgggccggcc accacaagca gcgcgcagcg cgccggcacc
     5761 attttggttt ggccgagatc gagctgttcg aggtcgagat tttggtcgcg cagcagggcg
     5821 gccagccgcg aatagccgcg gaggcgatca aaatccaccg gcgcgtgctc gccatggcct
     5881 tcgaggaaac acacgaccgg gcgggtggct tgcgtgagcg catggatggc gccgctaatg
     5941 agcgcctcgc cgcggaagag gcggcgcgga ttttcggtgg tgttggcggg gaggacatat
     6001 tccacgaggt ccgcggccgg gatggccaga tggcgcccgc cggtggccag caccacgcat
     6061 tcctcgccgg agaggttgaa ctgccgcgcc agctccttgg tgcgcgcgcc ggccaaatcg
     6121 gggtccacga actccacgtt gacgttgccg ctggcggcgg catattcctg caagagcagc
     6181 gcgagcgggc cataggcttc gtggccgggg cgcaccagca catgaagccg gatttcgttg
     6241 gcggtggctt ccagcaggcg gcggctggca tccgcgaggc gcaccgagtg cgccgcgcgc
     6301 caatgccagc gcgcgtagtg gcgctgcgcc agataattca ccatggcgaa gatggccacg
     6361 ctcaacagca cggccagcgc cacgttggcc cgcagcacga tgcgctgcag gcggctggcg
     6421 gcgagtgggc tggatggcag gagactcatg gccggttagc gcagacggcg cacctccagc
     6481 aggcggacgg ccgtgaacaa cagcagcacg gtggcgctgg cataccacag aatcacgcgg
     6541 gtatccacga tgcccaccgc gaaatcggcc atgtggcgcg gcgcggccag ttgatgggcc
     6601 aactgccgga ttgcggccaa cggcgcatgc agcggcagca ggccgccggt cagcagcaaa
     6661 ctcaatacgc ccaggcacgc catggcggcg atggcctgat ggcgggtcag cagcgagcaa
     6721 agcaaaccga tggccaggaa gagcgcgccg accagcgccg tgcccagata gcccgctgcc
     6781 acggggcccc aatccagcgg cggcaggtgc gcgccgcacc gccgcagtag aacggcgaat
     6841 accagcgtgg gcagccacag cgcgatgaac atcgtatagg ccgcggcaaa cttggccagc
     6901 accacggtgg ttccgccgat gggcgccacc agcagcgtgg gcaacgtccc catgcgccgc
     6961 tcttcggcaa acagccgcat ggtcagcagc ggggtcacca gcagaatggc cagccagtag
     7021 ccggtggagc cgaacaaatt gccggccaaa tcgcccgcca gcggcccctg cgcgagattg
     7081 gtggccagca cccacaagct aatgccggca atcgccagaa atgtcgtacc gatcacatag
     7141 ccgatgccgg tacggaaata ggccgccagt tcgcggaacc acaaagtcca aaacgcgctc
     7201 atcgggcgct ccttccgcgc ggattttgca cccgcggcgg cagcgtcgtc aggcgcagaa
     7261 acgcatcggt gaacgagccg gtgggcgtgg cggccgccaa ttgggcgggg gtgtcgttgg
     7321 cagcaagccg cccaccattc agcacgagca cccgctggca gagctgctcc gcctcggcaa
     7381 ggacgtgggt ggaaaaaaga attgtccggt ggccgcattg ctcgcggagc atttcgtgaa
     7441 agcgctgcgt ttggccggca tcgaggccgg ccaacggttc atccagcagg aggatttccg
     7501 gctcggtgag caggcaatcc gccatgccca cgcgttggcg aaagccgcgc gaaagcgtgc
     7561 caatcgggcg gcgggccatc tcgcccaagc cgcagggttc gagaacttgc cgtatgcgtt
     7621 ccgccagccg cttgccgcgc aagcctttca atcggccgcg aaacgccaga tattcctgca
     7681 cgcgcatttc gggatagagc ggcacatttt ccggcaggaa gccgatgcgc cggcgcacct
     7741 ccagccactg ggtcgtggga tcgaaaccgg ccactcgcac ccgcccggta gtcggcacga
     7801 gcaggccggt cagaatcagc aggagggtgg ttttgcccga cgcgttgggg ccgagaatgc
     7861 cggtcagttc gccgcgcgga acctggaacg acacgccacc cagcgcctcg cagcgcggga
     7921 aacgccgcgt cagaccgtcc acttcgattg ccagttccgt tgccgccatg gggagatatt
     7981 ccgcgcccac tctacccacc gcaaaaggct gaatcaatcc catatcccgg cggcggctgt
     8041 cgcgcgcggc gcagttttcc acagtgtgga aaatatattt ccacagtgtg gaaaacgggt
     8101 aggatgcgcg gcatgaggag caggctccaa agcggctggc tggcgttggc gctggccgcg
     8161 gtggtggcgg tggcgcagga gtcgtcgccg ccattgacct tgacgctgga ggacgcgatc
     8221 gagcaggcgc tggcgcacaa ccgcgatttg gtgcgtggcg cnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn ngcgcggcgc gtgtgggagc gggaccgatc ggcgttggtg
     8401 gtgcaggtgg tcgagctgtt tgaaacgctc atttatttgc ggcaccaagt gacctgcgac
     8461 gaagaatttg ccgaacgaat gagcggcctg cgtcaattgg ccgcggcgcg cgcggagcag
     8521 ggcaaagcct cgcggacgga tgtcctgcgc atggagtggc aagagggcga ggcgcagttg
     8581 cgcttggaaa ccggtcgagc gcagttggcc atccgttttc aggaattggc caatgtgctg
     8641 gggttgccgt tgacgacttc cttcacgctg gtgccgccgc cgttgctcga tttgaccacg
     8701 ggcgaaaccg atgaagcact ggccgtggcc ttggcggaac gtccggatta tgcgcaggcg
     8761 ttgcaggatg tggaaaccag cagccggcag gagcggctgg cgcggcgacg cctgctgccc
     8821 aatttgcggc tggcggccac gcaaaccact tatggcgagg gccccgaatg gagcgacgcc
     8881 acccggctcg atcaagagga ttggtttgtc ggcctgacgg cggatatgaa tttgaatctg
     8941 cgcggcacgc ggctggatgt agcgcgcgcg ggagtggatg ttgcggtccg cgaacacgtg
     9001 gcggaaattg tccgcaaccg gttggccgtg gaagtgcacg cggccttggc ggaatatcgg
     9061 cggtcgcgcg cggcgctggc gttggccgcg cgcaatcgcg agctggcggc cgatcgcgcg
     9121 gaattggcgc gcacactgtt caccgcgggc cgcgcgccgg cggacatggt gtccgacgcg
     9181 gaaacggatt ccagtagcgc catgctgcgc gagctggcgg cgcgccgcga agcagccgtg
     9241 gcggcctatc ggctgctgca tgtgctgggc accttggtgc ccgcgccgaa agaattgttg
     9301 cggccaacat cacaagggac ggagtcatga agaatgttgc gggatggcgc gggtggtgcg
     9361 gcgtggcgtt gctgttgctg gcgggctgcg gacgacccgc ggcgccggcg ccgcgggtgg
     9421 cggtagtcac gccgggcgcg attcgcatga cggttccgtt tgccggcgaa ttggcggcgc
     9481 ggcgggtgga gatgattacc gccggaatgc agggttcggc ggtattggcg gagctggttc
     9541 cggagggcac caaggtggcc gccggcgaat tgctggcgcg atttgacacc agccaatttg
     9601 cccaggattt ggcccggcag gagcatgagt tgatccgcgc gcggcaggaa ttagacagcc
     9661 tggccaaggc tgaattgccg ctggaattgc tcgatatgga aacccgccag caagatggtt
     9721 tggcggaggt ggcggcagag gagcgctttc tggaatcggc gcgcgacttg gccgcgcgtg
     9781 gcttgatgtc ggcagacgaa gtggcgcgac aggagcaaaa agtggcgggc ttgcgccagc
     9841 gcaacgagca atgggacacg cggattgcgc tgacgcgcga ccacgtgcac gcggcgcggc
     9901 tggccaaggc gcaagcggcg gtggaagctg ccgaacggca gcgcgatttt accgcgcagc
     9961 aggtggcgct gtgcgaggtg cgcgcgccgg tggccggcat tgtgacgctg gtgccgttgc
    10021 cgattggtgg ggagtatcgc ccggcgcacg ttggcgacac gctgtttcgc aatcaggcgt
    10081 tcatgtgcct gccggacccg gccgaacacg tgctgcgcgg ctatgtggac gaagcggaat
    10141 tgccctgggt gcagcccggc aaccgggtgg aagccacgcc agtggcctat ccgcatgccc
    10201 gcctgacggg gcgggtagag atggtaggcg gcatggcgca aacacgacca ggcgatcctg
    10261 cttggcgcaa gcgcttcccg gtggatatcg cgttggatcc gctgccggag ccgatgcctg
    10321 tgggcattag cgtgcgcgcg gatatcgtgg ccggtgagaa tacccatgcg ctgctgctgc
    10381 cgcgagaggc agtggcctgg catcatctgc aggcggttgt gcgccggccc aatggcgatg
    10441 aaatccctgt gcagaccggc ttggccgatg ctacccatgt ggagattgtt gccggactgg
    10501 cagcgggcgg cgaggtgctg cttccttgaa agtgttttgg gcagatattt ggacgggcct
    10561 gcgcaccggc cccgcgcggg ccctgttggc gctggtgagc ctggcgctcg gcttgtttgc
    10621 catgactatt ctgctggcga cgctggatgc cttgcagcga caggcggatc gcttggtaca
    10681 agcattcggc gccggcgcta ttgtgttgcg tccggaggct gggcaagcgc cgtgggggcg
    10741 agccatggtg gattacttcc gcgcgaatct gggcgcggat gcggcagtat ccggcgtgcg
    10801 aacgctgcct ccgccggcgg gcgcggattt tgcggtggcc gccggcgatg accaattggc
    10861 gcgggcgcgc gggtggcgca tcgtggccgg gcgcgcgctg gatggctggg atttacgcca
    10921 cgcagcgcgg gtggcgctgg cgccggccgg cttgtgccaa cgcaaaaact ggcgcgaggg
    10981 cgacttggtt ttgctgggcc atgagccgtt ccgcttggta gggtgctacg aagccggcgg
    11041 cgagccagtt ccggggttcg ccccggacac cgtatttatt ccggcgacgg ttgccgcgtt
    11101 gggcgccggc ccgcgcgatg aacgcggcga tatatccgcg ttactgttga ctgcggccgg
    11161 cacaaccgcg ccggaagatt tgcaacgccg cgcgcggggc ttgttgcgtg gtttcgggtt
    11221 ggacgataac gccgtggaat ggatcacacc agacaccttg ctgcgcgggg tgcgtcgctg
    11281 gcagcaggcc gtgggatggg tggcgggttc cggcgcggcg ttggggctgc tgctgggcgc
    11341 ggtgtcgctg gccggaatgc tgctgaccgg cgtgcgcgag cgtgtggctg aaatcgggct
    11401 gcgtcgcgcg ctcggcgcac gtccgcatga aatcgccctg ttgtttgttg cggaggcggc
    11461 ggtactgggc ctgggcgcgg cgttgctcgg tctgctggga gcgggtgcgg tgctgctggg
    11521 gcagggggcg cgcttcccgt tgccgtttca tttcggcgtg ggcgcatggc tgattccgct
    11581 cgtgttggcc ggcgggatcg tgatactctg ctcgttgggg ccggcgtggc aagccgcccg
    11641 cctgccgccg gcggaggcat tgcgcaatga ataaaagaga atggctgctg ctgttggtgt
    11701 tgctggcaac cagcgcatgc gcgcgggaag gagagctgga gatgagcaaa acgtgcatgg
    11761 gttcttcttt ggcaggggcg tggtatgagg ccaatccgga gcgcttgcgc gccgagttgc
    11821 agggctatct ggatgcggcg gaagtgacgc ccgacccgac tatttttgca gtgattgcgc
    11881 cgcacgcagg ctatgcgtat tcagggcctt gcgcggcggt ggccatcaag gcgttggccg
    11941 cgcgaaccaa cttgtcgcgt gtggtggtgg tggggtttgc tcaccgcgtc aggctggcgg
    12001 gccgcgccag cgtgccctcg caagaaacgt gctaccgctc gccattgggc gacacgccgt
    12061 tggacaccgc caccatcgcg ggcttgctga aaaagaccgt gttcgtggac aaggctgcca
    12121 cccgtgaagg cgaaaatagc gtggaaatga tgctcccgct gttgcaggtg gcgctgaccg
    12181 gccgcgagtg gcaactgctg ccgatcactc tcggccagct tgatgagcgc ggcttggccg
    12241 aggtggccga cgcgctcgcg ccgctgctgg atgggcaaac ggggtttgtg ttcagttctg
    12301 actttacgca ctacggcccg aactatggct atgtgccgtt ccgcaccaat atcgccggca
    12361 acctgcgcaa gctggatagc ggcgccgtgg aaaaaatcat ggcgggcgat gccgccggct
    12421 ttgccgctta ctgtcaggaa accggcgcga ccatttgcgg gcaggacagc atcggcgtgc
    12481 tgctgcggat gctgccggat aatttcactg cgcgcgagtt gttttacgac acctccgggc
    12541 ggcgcaccgg cgactttatg aattccgtca gctatgaggc catcgggttc tatggcgaag
    12601 gcccgaccat ccaccgaccc gcgccggcgg tagccgaatc tgccgcggcg gatcctttgt
    12661 ccgctgccga caagcaggcg ttgctggcca tggcccgaca ggcgatagcc cggacattcg
    12721 acggccagcc gccggcggat gccaaggcgc tgggcgcgga gctgtcgccg gccatgaaga
    12781 agaagatggg cggtttcgtc acgttgacca tcggcggcga attgcgcggg tgcatcgggg
    12841 aaatctttcc ccggcgcgcg ctggccgatg tggtggtgga ccacgcccaa gatgccgctt
    12901 tccacgaccc gcgctttccg ccgctgacta aaaaagagtt tgcgcaggtc aaaatcgaga
    12961 ttagcgcctt gaccccgccc gaaccggtgg cgtcataccg cgacatcgtg attggccgcc
    13021 acgggatggt gctggaactg cgcgggcgtt ccgccgtgtt tttgccccaa gtggccccgg
    13081 agcaggggtg ggacctcgcc accacgctga ctcatctggc ctataaggcc gggttgcccg
    13141 gcaacgcttg gcaggacccc aaggccactt ttacggtctt tgaggcggtg gtttttcacg
    13201 aataaaaggc ttttggacgt attgatgatt gtttgacaaa agcggcagaa aatgcctatt
    13261 ttacgactgg cgacttgaaa tcggcctatt tgtatgtaag gtttacgtta gcggatgtgg
    13321 agaactgtgt gagaaatggg aaggcgatta agaacgcggt gcgttcttgg cgttgacctt
    13381 aaagaaaact ggagtatccg tgagccgccg gcaaatagct aaaaaatcgg ggattctggg
    13441 cgcgttgctt tgcgccttgc tgccgttggg cgcgggggcc caaaataccg aaaccaagct
    13501 caccccgccg ctgtacatcg gcaacaccca gccggtgcgg gatacttttg gccgtgtgat
    13561 gcgcggctcg ccgaaccatc gtgaaggtcg tgcgctggtg gaagtgcgcg tggcaaccaa
    13621 ccgcatcgtg cgtccgccgt acaaggatgg cagcgcccat ccttaccatc cgttgctcaa
    13681 cggcgccgag ggggcggggg gcatcggcca gaattgcgcc aacgacaaca caggcttgtt
    13741 ctgccttgcg tttcacaagc gcccggctcc cggcacggtg gttttcgccc gcgtattcaa
    13801 tgcgccgacc gcgcaggagg ccacgttcta cgcggattct gagccagtgg aaattactgc
    13861 gctggatacc gccgtcgtgg tggaattcgg cgcgctaaag ccgctggatg ccgacgatga
    13921 cgacaacgac ggcctgaaca attcctggga aaaattgttg ggcaccgatg accggttgac
    13981 cgccgactac gacggcgacg gcatgagcga cttgcacgaa atgttggcgg gcaccgcgcc
    14041 cgatgatccg aactcacggc tggcgttcca caccgtgcat cgcgaaagaa cggcgcgcgt
    14101 ggtgaaagcg ggcgatccgc ccgtgcggcc catccatgtg tcgtggcaat ccgtgccggg
    14161 caagcgctac caactggaat atgtggcgca actggtgccg gatccagtca ctggcgaaga
    14221 acacgaaatc atccccatcg gcaacgtgat cacggccggc gaaaacgaat acgaaatcga
    14281 aaaatgcgtg gacctgccgg atgacgccgt gaccggcacc ttccgcattc gcctcgtgcg
    14341 ataaaccgcg cgcgcggctt gcctccgatt ctcctccggc aggagttttg tgcttctgtg
    14401 ccgggggacg ctttgctaga accccatccc gtggataccc ccgcccagat tcgtgtgttg
    14461 ccggacacgg tgatcaacca gatcgccgca ggtgaagtgg tcgaacgtcc ggcatccgtg
    14521 ctcaaggagc tggtggaaaa ctcgctggat gccggcgcgc ggcgcatcga ggtggatgtg
    14581 gtggatggcg gtcgccgtct tgtccgcgtg cgcgatgatg gcgcgggcat ggaccacgat
    14641 aatgcccttc tggcgctgga gcgccatgcc accagcaagc tgcgcgcgct gcaggatttg
    14701 gatcacatcg ccacgatggg cttccgcggc gaggccctcg ccgcgattgc ctccgtagcg
    14761 cagttcacgc tgctcaccaa ccgcgcaggc tcggccaccg gcacggagat tctggtattc
    14821 ggcgggcgca ttcaagaggt gcgcgatgcc ggcgtgccgc cgggcacgga catcgccgtg
    14881 cgcaatttgt tttttaatgt gccggcgcgc cgcaagtttt tgcgctcggc gcccaccgag
    14941 ttcacacaca tccggcaggc gttcatccac gaggcgctgg cgaatccatc cgtggaattc
    15001 atcttgcgcg cggacgatgc ggatgtgtac cggctgccgg caggcacgct gttggaccgc
    15061 atccgcgatt tgtatggcgc ggagatggcc gcccacctgc gcccgctgaa cttcggcaac
    15121 ggctcggtgg aaatcagcgg ctatgccggc gtgccaccgt ttagccggct ggaccgcgaa
    15181 tggcagattg tgttcatcaa tgagcgtccc accagttcgc cagtggtttc gtttgccatc
    15241 cagagcgcct accgcgatgt gctggtgggg gggcgctatg cgccgctgtt catgcaggtg
    15301 cgcgtgccgt ccgacgcggt ggatgtcaat gtccatcccg ccaaaaagga agtgcgcttc
    15361 caccgcccca ccgaagtgcg cgacgtcatt attgaggcta tccgcctcgc gattagcggt
    15421 ggcgaaatgc cgaaaggcaa acggtcggcc gccgcgccgc accgtgccga acctgtcgcg
    15481 cccgcgccgg cggcggaacc accgcccgcg gtagtcgcgc cagcggctgt ttcgcctgca
    15541 gccccgccac ccgcgccggt agtgccggcc ccggtggcga ccatccagca acaatggacg
    15601 tgggcatcca gccaaccctc ggccacgccc gatggtctgc ccgcgcgtgt cacgccatgg
    15661 gcggagtatc gtttgctggg ccgcgtggcg gggctctaca ttgtgttgga aacaccggat
    15721 ggcttggtgc tgatggatac ccgctcggcc cacgagcgtg tgatttatga acgcctgctc
    15781 cacgccgtgg accaaggccg cgcgccctcg caggggctcc tgccgccggt ggcggttccg
    15841 ctgtcgcccg cgcaaaccaa tgcgttgcgc aagcatttgg ccgcgctcaa gcaaatgggc
    15901 ttcggcgtgg cggagttcgg cgcggaaact ttcatggtgg atgcgctgcc ggtgtgggtg
    15961 gcgcacgcgg cgccggggcc gctgctgtcg gacattgccg atggtctgat gcaaggcggc
    16021 cgcgcgacgg ccaaagactg ggtgctgccg ctgattgccg aaacggccag ccggcaggcc
    16081 gtcagcctgc aacgcgaatt gaacgaagcc gagttgaatg cgctcatccg cgatttggcc
    16141 ggcacggaaa tgccctatac ttcgccgcgc ggcaaaccga cggtcatcct gttgagccac
    16201 cgcgagctca accgcaaatt cggccgcgag taggcgcggt tcagaacaac aagccggccg
    16261 ccagccacag caccatgcca gtggttgccg ccactttttg cagcagcaca atcaagccgg
    16321 ccaataccgc gccattggcg atgcgtaccg cgccgtccgc ttttttgagc cggtgttgtt
    16381 catagaaaaa taccggcaaa aagcagccca gcagcatccc tatcagattg ccgatgatcg
    16441 ggaccaccag gccgccggct accgcgccgc ccacgctgcc cagcagcgcc agccagccgg
    16501 cggcgcgcga accgccgcga cgctgcacgc cccagcgcac ggcaaaccat tcgccggtgt
    16561 ccaccgccac gcacaccacc agcattccga gcagcgtcgg ccagccggga aactcggcag
    16621 aaccggtcaa cagcgcggcc acgattccgg ccgccagcac caaccacgtg ccggagatgg
    16681 agaacatcgc caacagcagg cccgttgcgc acaggagcgc gcacacggcc cacgccagaa
    16741 aaactcccgc cgccgccaac ccgtggagca gggttgcggt catgtctact ccttgacccc
    16801 gcccgcggtc aggccgctga ccagcaattt ttgacaggcc agaaacagca gcgtaatggg
    16861 gaggccggcc agcaccgccg tggcggcaaa gtcgccccag cggtagttct gctcatagag
    16921 aaaactgttg gcgccgaccg ccagtgtcca ttgatccgtg cgctgcaaca ccacgctcgc
    16981 cagtggatat tcccctagta ggccgatgaa cgccaaaatg aacaccaccg caaaaatcgg
    17041 cagcgaagtc ggcagcatga tgcgcaaaaa aatctgccac ggcgatgcgc catccatcgc
    17101 cgccgattct tccaaggcca ccggcaatga atcgaagtag cccttgatca tccagatgtg
    17161 cgtggcgatg ccgccgagat aggcgaggat ca
//
LOCUS       DAOPNP010000048        16661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4133_length_16661_cov_8.131218, whole genome shotgun sequence.
ACCESSION   DAOPNP010000048 DAOPNP010000000
VERSION     DAOPNP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_16661_cov_8.131218"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1446
                     /locus_tag="PKI64_05890"
     CDS             403..1446
                     /locus_tag="PKI64_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOE00577.1"
                     /translation="MGLLLVCGQGWAGAPPFANEEGADRALIVLAAPLASDRYYCPVR
                     QDILDFQIGYARQILGRDNVVILADRATCRQLAKELPADILLEAPLRDIWTRDFLPVG
                     SGKSVLFRYSAAAQAGRQKEADWVQDGFIRLAQKMGLAIQPARWILDGGNVVDNGRDK
                     VIVTDRFLDDNRLGEAEAVALLREQLGVAHVAVLPADPEDTLGHADGMVAFVASNTVA
                     VARANGADIEPIRVALRGAFPGIEIIEIDVVLDEDDFDPEYGSARGLYVNATLTDHYL
                     YVPVFGQPSDATVLACLRAASDREVVPVDAAKVSRLGGSVRCLGAQMKGDNARRLIEA
                     ARRPKGVGPKGKK"
     gene            complement(2005..2796)
                     /locus_tag="PKI64_05895"
     CDS             complement(2005..2796)
                     /locus_tag="PKI64_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE00578.1"
                     /translation="MIIDFHAHAFADTLAPKAIPALEAGADVKACTDGRVNSLLASMD
                     RAGIDRAVVCPIATKPTQYAGIRQWAHAVRAAHPRLEMLLSIHPEDPAALAHLDEIAA
                     DGFKGIKLHPYYQCFKIDEDRMFPIYERLAAHGLLVVFHTGFDIAYPFDRIADPVRVR
                     RVADTFPTLKLVATHLGGWKDWAESRRHLIGQPIYIETSYCLNELPPAEVRAMLMAHP
                     ADYILFGTDSPWNDQSEELARWRALDLPPDRLAAALGRNAARLLA"
     gene            complement(2793..3590)
                     /gene="surE"
                     /locus_tag="PKI64_05900"
     CDS             complement(2793..3590)
                     /gene="surE"
                     /locus_tag="PKI64_05900"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOE00579.1"
                     /translation="MRILISNDDGIQAPGIHALHGAVKDLGEIHVVAPNGERSAIGHG
                     ITLTKPLHARPWPPGGPHFGTAISGTPADCVKLAVSLLLDPKPDLILSGINLGPNAGI
                     SVLYSGTVSAAAEGPIMGIPAIALSLDTFSEPNWATAARVSRYLVEQVVQGRLQIAPD
                     IFWNVNIPNLPYEQLAGIRITRMGSSRFIEKYECRHDPWGNPYYWMSGELYEKGDMQG
                     TDLQALREGYVSLSPIGLDHTEHAAIEALAAHPLILPTPAPAAVTTG"
     assembly_gap    4601..4700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4701..7352)
                     /locus_tag="PKI64_05905"
     CDS             complement(<4701..7352)
                     /locus_tag="PKI64_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOE00580.1"
                     /translation="MSSSIRRSSRREPPVWRLVLSLAMLWTLTAGAAELSVEFTYSPA
                     DVTLTPAGAYTAVQLTGGARVVDEAGAPAIPAVFANILLPAGAENISCTAAGEWLPLA
                     NDITPTPAQPRRPKSRPKTAFVPPNDRYAAATPWPADLAALQGVHDMQGHRFISVRLN
                     PLAYIGATRTLFLREKITVTVRYDLAPARRVAPRQAALFGPLVQSLVVNPDALDTAAP
                     PATMAAPKATLDYLIITAPALSNAFQQLANYRATAAGGAYSTRVMTTNQIAASFPGAD
                     LPAKIRACISNSVANSGTIMVLLGGDDTIVPVRYCYATAEGDTETNMPTDLYYSGLGG
                     NWNANGNSIFGEVTDQVDMAWDVVVGRLPMRTVAHVSNYVARLIAYEAGAPPTNRLLL
                     GGTKAWYTYSGTQRPSDNVTGDGHPGFRSTSPPHTYVSDSEAWLRRLFRDGIRSNWPA
                     KPSLLCDTITSWDKSSCGDHPQNRTNTIAAFNRNWTHLMFSGHGSPQNWGLEDSSFGH
                     VQADSLTGMAAVVYTDACLTGHFDKNSNKIDGTTYTTEPCLGEAFLRNAALRGGALAY
                     IGCARYGWGDQDPPPADNYARGGPSTEYAYRFYQRLYETNRTLGVAFAMHKADMISLS
                     GINGCERWIQFGLNLLGDPALRMPGTPSDLAAPAFAANPGPLAATAGVTVAFTVAAGG
                     KPAPVLTLLNTTALTGTYTFSPTSGQLVYTPRATDLGERTFTFRATNLLGSATQVVSV
                     TVQATPPAAPATLWASATNTTDFTAAWSVVSNANIYRLDVGTNTTFTGAGGTPYFVPG
                     YSNRNLTSTSFSVTGLTAGATYFFRVRAVNSGGTSPNSATASVTTLAIIPTPPPARIS
                     APVAIGNGLTITIPAVVGVAYQLQYATN"
     gene            complement(7555..8283)
                     /locus_tag="PKI64_05910"
     CDS             complement(7555..8283)
                     /locus_tag="PKI64_05910"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOE00581.1"
                     /translation="MNILSQGDCLQPGVYTVHTRFERSILLFNRAGRALFVVPPAIGP
                     GPLNIVVARPHAFVTTDTLTLPARGAAPIYDSRLPRLPAAAREQVVRVLRAALPRVAP
                     PDSLATLFWPRPQLPPFQQRRDQRIRNALRAAARGNLAAGVRRIRGCGAGLTPSGDDF
                     LAGWMLACRLRRNLATARQIHQAARGNNRIANLFLDLAARGRIHAPLNQFLQKPTTAR
                     ARRVCAFGHTSGADLLCGLFYGLA"
     gene            8497..9450
                     /locus_tag="PKI64_05915"
     CDS             8497..9450
                     /locus_tag="PKI64_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00582.1"
                     /translation="MIKVEHLVKKFDLRTAVDDVSFGVEKGTVLGFLGPNGAGKTTTI
                     RMIAGYLPPTSGTVMVNGVDVVANPVAAQRQIGYMPENTPLYDDMTVSGFLRFIAELR
                     GLRGKARDQRVADIMERCMLTSVRHQPVDTLSKGYRRRTGLAQTLLHDPAVLLLDEPT
                     EGLDPNQKQVVREMIEQMAKDKIIMLSTHVLEEVEALCTRIMIISEGRLKLDSTPEDL
                     KKRSASYNTLTLDVVAPGADPATVLAQIPDVAQVDVVQRAGDKHVLRLIPRDKQSLAV
                     SAIEVATQHKWLVTELRADPGRLDDVFRDVTTTADVIRQSA"
     gene            9472..10215
                     /locus_tag="PKI64_05920"
     CDS             9472..10215
                     /locus_tag="PKI64_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00583.1"
                     /translation="MRVCNQVRAVAKREWSAYFNSPVAYIFIIIFLALAGFFTFSVSN
                     FYEAGQADLSRFFFWHPWLYLILVPAVAMRLWAEERRLGTMELLFTSAVTPGQAILGK
                     WLAAWAFLALALALTFPIPLTAAWLGQPDWGVIAAGYVASLLLAGAYLAVGMFTSALT
                     RNQVISFILAVVLGLFLILAGFQPVTELLSQWAPGWLVDGVAAFSFMPHYEAMQRGVL
                     DLRDFVYFGSVVGFMLFLTHLVLGNRSGR"
     gene            10243..12129
                     /locus_tag="PKI64_05925"
     CDS             10243..12129
                     /locus_tag="PKI64_05925"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOE00584.1"
                     /translation="MKKTTSKLFTGLAGAFVVLGILVVANILLSSVRLRTDLTAERLY
                     TLSPGTVAMVRELERPVTLKFYFTKGNPNIPVPLKNYVQRTQDFLRELVARSGGKLAL
                     EMLDPQPDSDAEEWAQRYGLLPQATGRLGAQPDFYLGLVAVAGTREAAIPVLDPSAES
                     QLEYLVVRLVQEVTQSRRPRIGIMSSLPALAEETMPFLPPSQQKQDWLFVTELKQQYT
                     VVSVPLDAKEIADDIDTLLVIHPKGISQPTLFALDQFVLRGGHLVAYLDPQCIADAPS
                     DEMRGMPPPSSDINGLAQAWGVTVDVSRVVADVAAATPINIGEGRAERLPAWLTLRGA
                     ENLNPAEITTGSLESLMVPFAGVIQGKPAEGLQMTTLVTASSNAVTLNLYQARNALDA
                     STRDGIPAPNAAIAVQLTGNFTTAFPDGPPAAEGEEADADKPADTNTWLKASAAPGAV
                     VMVADADILVTDFMARGINFFGRTLYQPFNDNLNFTLNIVEQMSGSPALIGLRGRGKF
                     DHPFDRVLALEKAAQEQWQAQEEQLQQKLMETQQRLNELQAAKSDDQQLVLSAEQKAE
                     LEKFRQQRFETQRQLKDVRKNLRHSIESLGLRLKVINLMAVPLVVAVFGVVLGWRRRR
                     RAVA"
     gene            12145..13323
                     /locus_tag="PKI64_05930"
     CDS             12145..13323
                     /locus_tag="PKI64_05930"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOE00585.1"
                     /translation="MSKKSLWVLVLVLVALGALLWWQGRQEAQPVTASAQAATAPLLA
                     GVDWNGLTTIMLADGVATTHLAKVDGNWTVVEQDGYPADVNRLRQMLRSLDDLAAGEV
                     VAAATDDLTEFGLVPAGDSPPTRLLLEQDGRGSTLWLGNMRAPKNDMQMWMRESGRYV
                     RVNDGPVRLIKDSMARISTTPDEWWERQIVAVEPADVDEVAVTAGDESYTIARDDTGV
                     YAVRDGADGETVDEAAAERLFGLLRDLRADTLLSAERAAELEFAPVATYRAETAAATY
                     TVEISEALADENNGWPLRLTVTAPNGEPPAAHKSAGRTYLVAPYIADQFKTPRGVLVK
                     AAPEPEEAAEAAAGEEPAENAEESADETPAEIAPPPVPDAAAEEPATPAESAAEAAPE
                     "
     gene            13385..14083
                     /locus_tag="PKI64_05935"
     CDS             13385..14083
                     /locus_tag="PKI64_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOE00586.1"
                     /translation="MSTKRQHEIGCPFCGREQTAELWDAIDAGQDPELRTALLAGQIN
                     RVTCAGCGKSFRIEKALVYHDREQEIFVHYEPLAGGITQPQAEQNFRAALQELARLLP
                     KDMPEWETHLVLTWNELIERIYLLEEGLDARLVEHIKYMMYQQNPAKLPAANKNLLFN
                     AQDSTDDQLCFVVQDRQTQKLEAVLNFARADYEALVNVFDSDEQLALLEEQFPGPYYN
                     GRQRFLRDQLDAED"
     gene            14086..14754
                     /gene="nth"
                     /locus_tag="PKI64_05940"
     CDS             14086..14754
                     /gene="nth"
                     /locus_tag="PKI64_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOE00587.1"
                     /translation="MATESLAQKQRRARTILRKLRQTYPDAKCELDWSTPIELTVATI
                     LSAQCTDRRVNMVTPALFKKYRTACDWAATPAATLEDEIRSTGFFRNKTKNIRALMAR
                     LDQEFGGQLPDDFETLVKLPGIGRKTANLLVATVFGKPGLVVDTHFIRLSNRLGFVKN
                     ETDAVKIEYALQKIVPSAEWTNWSHVMVFHGRRCCYARKPECARCPIQAQCPAAKTFL
                     AAAT"
     gene            14760..16355
                     /gene="murJ"
                     /locus_tag="PKI64_05945"
     CDS             14760..16355
                     /gene="murJ"
                     /locus_tag="PKI64_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOE00588.1"
                     /translation="MENRNVIRFAFAVGGFTMLSRVLGMVRDVLTARMFGTLPVMSAF
                     VVAFRLPNLFRALFGEGALSSAFIPVFMESRRTEGEAPAWRLARRVSSLVGAVLLGII
                     VVGIAGMGLARHWVTVGSKAANVLPLAQIMLPYVFFICMAALAMAVLNSYRKYSVSAF
                     TPALLNITWILSILFIVPLVRGGTSMQIRALAWTVFVAGAVQLVYQVPSLLRVGWRPG
                     VDTDWRDPRVKQVFLLMGPAALGLAVNQINVMINSMIALWAGDWAPSALFYAERLIYL
                     PQGILATSLGTVLLPVLSDFAVQKRHDDMRAAIHHGLRTLLFVMTPAAVGLFVLAGPI
                     VQMLFEQGAFDAFSTVLTARALAFYAPGLMLFCLAKVFVPSFYALQDTRTPVKVGLCA
                     VTLNLTLNAIFALTLPEYWRHAGMALATGIAEGFNGLTLAWLLRRRLGPFGVRGILGG
                     VGRALLAAGAMALVAWQVQRVGTTWLYLHVSPKTAQLIGVPAAIALGVGIYFLIARFA
                     GFPELGFVLEALRARRQERAAAQ"
     gene            complement(16392..>16661)
                     /locus_tag="PKI64_05950"
     CDS             complement(16392..>16661)
                     /locus_tag="PKI64_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE00589.1"
                     /translation="EVNLKYMEPSYVIRSVRANSQDARLCDDFARNAVHAAMAGKTNL
                     IIGDWYSVYVHVPVALATASRKKVDPEGDLWRAVIATTGQPPTWD"
BASE COUNT         3294 a         4362 c         5877 g         3028 t
ORIGIN      
        1 caacgcccaa aggaaaccga tcctcttttg ccttctcatc ctatctgtgg aaaaagcatg
       61 gaaagccgaa gacaattaac gcaagcgatg cgaacaaaac caggaacaat taacgcaagc
      121 ggcgcaagag atacataacg acgcaaagaa agccaaaggc agtcaacgcc agaggcgcga
      181 gagaaacata acgacgcaag cgagagacaa agaaagattc tcgccaaggc gcgaagggcg
      241 caaaaaaatg ggggggggca ctgcgcgcgc ctacagttgg acaacagcca ggggcagggc
      301 cgccagctat tgcccggttc cccagtctct gcttggtggg gaaatggttt gcgctataat
      361 gccgtcatgt ggcgtggtgt gtggcgaacg tgttggcgag ggatggggtt gctgttggtg
      421 tgcggccagg gctgggcggg cgcgccgcct ttcgccaatg aggaaggcgc ggaccgcgcc
      481 ctgattgtgc tggccgcgcc attggcgagc gaccggtact actgccctgt tcgccaggat
      541 attttggatt ttcaaattgg ctatgcccgt cagattttgg gacgcgacaa tgtggtgatt
      601 ttggcggatc gggccacgtg tcggcaactg gccaaggaac tgccggcgga catcctgctg
      661 gaggctcctt tgcgggatat ctggacgcgc gatttcctgc ctgtgggctc gggaaaatcc
      721 gttctttttc gctattccgc cgcggcgcag gccggccgcc agaaggaggc ggactgggtg
      781 caggacggtt tcatccgtct ggcccaaaaa atggggctcg cgattcagcc ggcgcggtgg
      841 attctcgatg gtggcaatgt ggtggacaac gggcgggaca aagtgatcgt gacggaccgc
      901 tttttggacg acaatcgcct aggggaggcc gaagcggtgg cgctcctgcg ggagcaactc
      961 ggggtggcgc atgtggcagt tctgccggcc gatcccgagg ataccttggg acacgccgac
     1021 ggcatggtgg ccttcgttgc cagcaatacg gtggcggttg cccgcgcgaa tggggccgac
     1081 atcgagccga tccgggtggc gctgcgcggc gcatttcccg gcattgagat catcgaaata
     1141 gacgtcgtat tggatgagga tgactttgat cctgaatatg gctccgcgcg cgggctctat
     1201 gtcaatgcca ccttgacgga tcattatctc tatgtgccgg tgttcggcca accttctgat
     1261 gcgacggtct tggcgtgtct ccgcgcggcc agtgatcggg aggtggtccc ggtggatgcg
     1321 gccaaggtgt cgcggctggg cggcagtgtc cgctgtcttg gcgcgcagat gaaaggcgat
     1381 aacgcgcgcc gcctcattga agcggcccgc cggcccaaag gggtcggtcc gaaagggaaa
     1441 aaataaaacc gagcaggaaa gcaggaaaat caggaaaaca ggaaaaggga gtgcttggtt
     1501 gaacagccga aaaaaatgaa aactgtcaac gctagagacg cgaacaaagc cgaagacaat
     1561 taacgcaaga ggcgcaagag atacataacg acgcaaggaa aaccgaagac agtcaacgca
     1621 agagacgcga acaaaagcag gaacaattaa cgcaagggac gcaagagata cataacgacg
     1681 caagcgaagg acgaagaaag tttctcgcca aggcgcgaag ggcgcaaaga gggagaaaat
     1741 aaaaccgagc aggaaagcag gaaaatcagg aaagcaggaa aatcaggaaa gcaggaaagg
     1801 ggagtgcttg gttgaacagc cgagggccga agacggtcaa cgcaagagat acataacgac
     1861 gcaagcgaag gacgaagaaa gtttctcgcc aaggggcgaa gggggcaaag aaaaatctag
     1921 cgagagttgt atttggatta gactttgggt cgtttgcggc cgttagcgcc tctggcgtct
     1981 tttgcgttag ataaggatcg agtgttaggc gaggaggcgg gcagcgttgc ggccgagggc
     2041 ggcggcgagg cggtcggggg ggaggtcgag ggcgcgccag cgagcgagtt cttcggattg
     2101 gtcgttccac gggctgtcgg tgccgaataa aatgtagtca gcagggtgag ccatgagcat
     2161 ggcgcggact tcggcggggg gaagttcgtt caagcagtag gaggtttcaa tgtagatggg
     2221 ttggccgatc aggtggcggc gggattctgc ccaatccttc cagccaccca gatgggtggc
     2281 gacgagtttg agggtgggga aggtgtcggc cacgcggcgc acgcggacgg ggtcggcgat
     2341 gcggtcaaag gggtaggcga tgtcgaagcc ggtatggaag accacgagca ggccgtgggc
     2401 ggcgaggcgt tcatagatcg ggaacattcg gtcttcatca atcttgaagc actggtagta
     2461 gggatgcagc ttgatgcctt tgaagccatc ggcggcgatt tcgtcgaggt gggcgagggc
     2521 agcggggtct tcggggtgga tggagagaag catttcgagg cgggggtggg cggcccggac
     2581 ggcgtgggcc cactgtcgaa tgccggcgta ttgcgtgggt ttggtggcga tggggcaaac
     2641 gacggcgcgg tcaattccgg cgcggtccat ggaggcgagc aggctgttca cgcggccatc
     2701 ggtgcaggct ttgacgtcgg cgccggcttc gagggcgggg atcgccttgg gggccaaggt
     2761 gtcggcgaag gcgtgggcgt ggaagtctat aatcatccag tggtgacggc cgcgggggcg
     2821 ggcgtgggga ggatgagggg gtgggcggcc agcgcttcaa tggcggcgtg ttcggtgtga
     2881 tcgaggccaa taggggagag ggagacatag ccttcgcgaa gggcttggag gtcggtgcct
     2941 tgcatgtcgc ctttttcgta gagttcgccg gacatccagt agtaggggtt gccccagggg
     3001 tcgtggcggc attcgtattt ttcgatgaag cgggaggagc ccatccgggt gatgcggatg
     3061 ccggcgagct gctcgtaggg gaggttgggg atgttgacgt tccagaaaat gtcgggcgcg
     3121 atttgcaagc ggccttgcac gacttgttca acgaggtagc ggctgacgcg cgcggcggtg
     3181 gcccagttgg gttcggagaa ggtgtcgagc gagagggcaa tggcggggat gcccatgatg
     3241 gggccttccg cggcggcgga tacggtgccg gagtagagga cgctgatgcc ggcgttgggg
     3301 ccgaggttga tgccgctcaa gatcaagtcg ggcttggggt cgagcaggag gctgacagcc
     3361 agtttgacgc agtcggccgg ggtgccgctg atggcggtgc cgaagtgcgg gccgccgggg
     3421 ggccaggggc gcgcgtggag gggcttggtg agggtgatgc cgtggccgat ggccgagcgt
     3481 tcgccgttgg gggcgacgac atgaatttcg cccaaatcct tgacggcgcc atggagggcg
     3541 tggatgccgg gggcctgaat gccgtcgtcg ttggaaatca gaatgcgcat gatgggcgat
     3601 gatggccgag ctccggtttc ttggcaaggg cagaaaagct ttgcgccgac aaagcccaag
     3661 acaattaacg caaaagacgc aagagataca taaagacgca aagaaaacgg aaaccagtta
     3721 acgcaaggga cgcaagagat acataaagac gctaaagaaa ataaaaccga gcaggaacac
     3781 aagagaatca ggaaaacagg aaaagagatt gattggttga acagccgaag aaatgaagat
     3841 agtcaacgca agaggcgcga ataaaaccgg ggacaattaa cgcaagggac gcaagagata
     3901 cataaagacg ctaaagaaaa taaaaccaag gcaggaaaac aggaaaatca ggaaaacagg
     3961 aaaagagatt gattggttaa acagccgaaa aatgaagaca gtcaatgcaa gaggcgcgga
     4021 caaagccgaa gacaattaac gcaagagacg caagagatac ataaagacgc aaagaaaacg
     4081 gaaaccagtt aacgcaagag gcgcaagaga tacataagga cgcaagcgga agatgaagaa
     4141 agattctcgc caagacgcga agggcgcaaa gagggagaga aaataaaacc aagcaggaaa
     4201 acaggaaaag agactgattg gttaaacagc cgaaaaatga agacagttaa cgcaagaggc
     4261 gcaagagata cataacgacg caaagaaaac tgaaatcagc taacgcaaca ggcacatcaa
     4321 tcgcatgcgt ctgatagcgt ctcttgtgcc acttgcgtca aaaattattg tttttcggag
     4381 gcttgttatg gctgtttcag cctcttgctc ttttcctgct ttcgtgggtt tcctgatttc
     4441 ctggttgggt ttcggatcgc ttgcgtctga tagcgtctct tgcgtttaag gctctgatca
     4501 gattttggat tgctggcggg gttaggggcg gatgacgcgg taatagcgtt ggcggttggt
     4561 gggggtggcg tcgcgcaggg ggagggtgtt gttggtggcg nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn gttggtggcg tattggagtt ggtaggccac gccgaccacc
     4741 gctgggatgg tgatggtgag cccgttgccg atggcgactg gcgcggaaat gcgggcgggc
     4801 ggcggggtgg gaatgatggc gagggtggtg acgctggcgg tggcggagtt ggggctggtg
     4861 ccgccgctgt tgacggcgcg cacgcggaag aagtaggtcg cgccggcggt caggcccgtc
     4921 acgctgaaac tggtgctggt gaggttgcgg ttggaatagc cggggacgaa gtagggcgtg
     4981 ccgccggcgc cggtgaaggt ggtgttggtg ccgacatcca gccggtagat gttggcgttg
     5041 ctgacgacgc tccacgcggc ggtgaagtcc gtggtgttgg tggcggaggc ccacagggtg
     5101 gcgggggcgg cggggggcgt ggcttgcacg gtcacgctga ccacctgcgt ggcgctgccg
     5161 agcagattgg tggcgcggaa agtaaatgtg cgctcgccca gatcggtggc gcggggcgta
     5221 tagacgagtt ggccgctggt gggggagaaa gtataggtgc cggtgagggc ggtggtgttg
     5281 agcagcgtca gcacgggcgc aggtttgccg ccggcggcca cggtgaaggc cacggtcacg
     5341 ccagcggtgg cggcgagggg gccggggttg gcggcgaacg cgggcgcggc gagatcggac
     5401 ggcgtgccgg gcatgcgcag ggcgggatcg cccagcagat tcaggccgaa ttgaatccac
     5461 cgctcgcagc cgttgatgcc ggagagactg atcatgtcgg ccttgtgcat ggcaaaagcc
     5521 acgccgagcg tgcggttggt ttcgtagagc cgctggtaaa aacggtaggc atattccgtg
     5581 gaggggccgc cgcgggcgta gttgtcggcg ggaggagggt cttggtcgcc ccagccgtag
     5641 cgtgcgcaac cgatataggc caatgcaccg ccgcgcaggg cggcgttgcg caagaacgcc
     5701 tcgccgagac acggctcggt ggtgtaggtg gtgccgtcaa ttttgttgga gtttttgtcg
     5761 aagtgaccgg tcaggcaggc atcggtatag actaccgcgg ccatgcccgt gaggctgtcg
     5821 gcttgaacgt ggccaaacga agaatcctcc aaaccccaat tttgcggcga gccatggccg
     5881 gaaaacatca gatgcgtcca gttgcgattg aacgcggcaa tggtgttggt gcggttttgg
     5941 gggtgatcgc cgcaggaaga tttgtcccac gaggtgatgg tgtcgcacag caacgaaggc
     6001 ttggcgggcc agttggagcg gatgccgtcg cgaaatagcc ggcgcagcca ggcttcggaa
     6061 tcgctgacgt aagtatgtgg agggctggtg gagcggaagc cgggatggcc gtcgccggtg
     6121 acgttgtcgc tggggcgttg agtgccggaa taggtatacc acgctttggt gccgccgagc
     6181 agcaggcggt tggtcggggg cgcgcccgct tcgtacgcaa tgaggcgggc gacatagttg
     6241 ctcacatggg cgacggtacg catgggcagg cggccgacca cgacatccca cgccatgtcc
     6301 acttggtcgg tgacttcgcc aaagatggag ttgccgttgg cattccagtt accgccgagc
     6361 ccggagtagt agaggtcggt gggcatgttg gtttcggtgt cgccttctgc ggtggcgtag
     6421 cagtagcgga cgggcacgat ggtgtcatcg ccgccgagca ggaccatgat ggtgccggag
     6481 ttggcgacgg aattgctgat gcacgcgcgg attttggcgg gcaggtccgc gccggggaat
     6541 gacgcggcga tttggttggt ggtcatcacg cgcgtggagt acgcaccgcc ggcggcggtg
     6601 gcgcggtagt tggcgagttg ctgaaaggcg ttgctcaggg cgggagcggt gatgatcaga
     6661 taatccagcg tggccttggg cgcggccatt gtggcgggtg gcgccgcggt gtccagcgcg
     6721 tcgggattca ccacgaggct ttgcacgagc gggccgaaca gcgctgcttg ccggggagcc
     6781 acgcggcggg cgggggcgag gtcataacgc acggtgacgg tgattttctc gcgcaggaaa
     6841 agcgtgcggg ttgcgccgat ataggccaac ggattcagcc gcacggaaat gaagcggtgg
     6901 ccttgcatat cgtgcacgcc ctgcaaggcg gcgagatcgg caggccaagg ggtggcggcg
     6961 gcatagcggt cattgggcgg cacgaaggcg gttttggggc ggctcttggg acgacggggc
     7021 tgggcggggg tgggggtgat gtcgttggcg agcggcagcc actcgccggc ggcggtgcag
     7081 gaaatatttt ccgcgccggc gggcagcagg atgttggcga acacggcggg gatggcgggt
     7141 gcgcccgctt catccaccac gcgcgcgcca ccggtcagtt gcacggcggt gtaggcgcca
     7201 gcgggtgtca gggtgacatc agcgggggag taggtgaact ccacgctcaa ttccgccgcg
     7261 ccggcggtca gcgtccaaag cattgcgagg ctcaacacca gccgccagac cggcggttcc
     7321 cggcgtgagg aacggcggat tgatgaactc atccaaccca taaccggagt ctaaccgaac
     7381 tttaacaatt ccgctatgac aatcggttgc cattcaccgt caattattca gcaacatgcg
     7441 tccatggctg ttcttgcatg tttcgggagg acgggaatga ttgagggagg aaaccttctc
     7501 gccgggaacg gcaggaaggt cagcgaccgt tccagtgcga caatccaacc cggatcaagc
     7561 taatccgtaa aaaaggccgc agagcagatc ggcgccggaa gtgtggccga aagcgcatac
     7621 gcggcgtgcc cgggcggtgg tgggtttctg caaaaactgg ttgagcgggg cgtggattcg
     7681 cccgcgcgca gccaaatcca aaaacaagtt ggcgatacgg ttattgccgc gggcggcctg
     7741 atggatttgt cgcgcggtgg cgaggttgcg gcgcaggcgg caggccagca tccagccggc
     7801 caaaaaatca tcgccggagg gggtgaggcc ggcgccacag ccgcggatac ggcgcacgcc
     7861 cgcagcgaga ttgccgcggg ccgcggcgcg aagggcattg cggattcgct gatcgcgccg
     7921 ctgctggaag ggggggagtt gcggccgggg ccaaaacagc gtggctaggc tgtccggcgg
     7981 cgcaacccgc gggagggcgg cgcgtaggac gcgaacgacc tgctcgcgcg cggcggcggg
     8041 caggcggggc aaacgggagt catagattgg cgcagccccg cgcgcgggca gggtcagcgt
     8101 atcggtcgta acgaacgcat gggggcgcgc caccacgatg ttgagcggcc cggggccaat
     8161 ggcgggcggc acaacaaaca atgcgcgccc ggcccggttg aacagcaaaa tgctgcgttc
     8221 aaaacgggtg tggaccgtat aaacgccggg ttggaggcaa tcgccttggg ataaaatatt
     8281 catggacaaa atgtcgcgca attagtgcga ataaatgtca cgaaatcgcc ccggagcgca
     8341 acctttgttc acaaaaaagt cggcaaaaca tggtttttaa cgggtataac ccgattcttt
     8401 acaagttgtt gtggattctg gcatgtaccc tgctcatggc aaaacgcatg ggcaaaaagc
     8461 ccttttggac tgagccaagg agcaaaggac gcaagcatga tcaaagtaga gcatttggta
     8521 aaaaagtttg atttgcgaac ggccgtggat gatgtgtcgt ttggcgtgga aaaagggacg
     8581 gtgctgggct ttctggggcc gaacggggcg ggcaagacga ccacgatccg gatgatagcg
     8641 ggctatttgc cgccgacctc cggcacggtg atggtgaacg gggtggatgt ggtggccaac
     8701 ccggtggcgg cgcagcggca aattggctac atgccggaaa acacgccgct ctatgacgat
     8761 atgaccgtga gcggctttct gcggtttatt gccgagctgc gtggcctgcg cggcaaagcg
     8821 cgcgaccaac gcgtggcgga catcatggag cgctgcatgc tgacctcggt gcggcaccag
     8881 ccggtggaca cgctatccaa gggttaccgg cggcgcacgg ggctggcgca aacgctgttg
     8941 cacgatccgg ccgtgctgct gctcgacgag ccgaccgaag gcctcgaccc caaccagaag
     9001 caagtggtgc gcgagatgat cgagcagatg gccaaagata aaatcatcat gctctccacc
     9061 cacgtgctgg aagaagtgga ggcgctgtgc acgcgcatca tgatcatcag cgaggggcgg
     9121 ttgaagctgg atagcacgcc cgaagacctg aagaaacgca gtgccagcta taacacgctg
     9181 acgctcgacg tggtggcgcc gggcgcggat cccgcgacgg ttttggcgca gattcctgat
     9241 gtggcccaag tggatgtggt gcagcgcgca ggtgacaaac acgttctgcg cctgatcccg
     9301 cgcgacaagc aatcgttggc ggtcagcgct atcgaggtgg cgactcagca caaatggctg
     9361 gtgacggagc tgcgcgccga tcccggccgc ctggatgatg tgttccgcga tgtgaccacc
     9421 acggcggatg tgatccgcca aagcgcgtga gtgaggaaag gagtagccag catgagggtg
     9481 tgcaatcaag ttcgggccgt tgccaaacgc gagtggagcg cgtacttcaa ttcgccggtg
     9541 gcctatattt ttattattat ctttttggcg ctggcgggat tcttcacgtt ttccgtcagt
     9601 aatttttatg aagcaggtca agcggatttg agccgcttct ttttctggca tccgtggctt
     9661 tatctgatct tggtgccggc ggtggccatg cgcctgtggg cggaggagcg gcggctgggc
     9721 acgatggagt tgctgttcac gtcggccgtg acgcccgggc aggccattct gggcaaatgg
     9781 ctggcggcgt gggcgttctt ggcgctggcg ctggcgttga cgtttcccat cccgctgacc
     9841 gccgcgtggc tgggccagcc ggattggggc gtgattgccg cgggctatgt cgccagcttg
     9901 ctgctggctg gcgcgtatct ggcggtgggg atgttcacca gcgcactgac gcgcaatcag
     9961 gtcattagct tcatcctggc cgtggtgctg ggcctgttct tgattttggc gggttttcag
    10021 ccggtcaccg aactgctaag ccagtgggcg ccaggatggc tggtggatgg cgtagccgcg
    10081 ttcagcttca tgccgcacta tgaagccatg cagcgcggtg tgctggacct gcgcgatttt
    10141 gtgtacttcg gcagcgtggt gggcttcatg ctcttcctga cccacctggt gttgggcaac
    10201 cggtcaggac gctgagcggc cgacgaaaag gatggtgcaa agatgaaaaa gacaacgtcc
    10261 aaattgttca cggggctggc gggggcattc gtggtgctgg ggattttggt ggtggcgaat
    10321 atcctgctgt ccagcgtgcg actgcgtacc gatttgacgg cggaacggct ctacacgctc
    10381 tcgcccggca cggtggccat ggtgcgcgag ctggagcggc cggtgacgct gaagttttat
    10441 tttaccaaag gcaatcccaa cattcccgtg ccgttgaaga actacgtgca gcgcacgcag
    10501 gatttcctgc gcgagctggt ggcgcgcagt ggcggcaagc tggcgctgga aatgctggac
    10561 ccgcagccgg attcagacgc cgaagaatgg gcgcagcgct acggccttct gccacaagcc
    10621 accgggcggc tcggcgcaca accggacttc tacctcggcc tagtggcggt ggcgggcacc
    10681 cgcgaggccg caatccccgt gctggacccc agcgcggaat cgcaactgga atacctcgtg
    10741 gtgcggctgg tgcaggaagt gacccaaagc cggcgcccgc gcatcggcat catgagctcg
    10801 ttgccggcgc tggccgaaga aaccatgccc ttcctgccgc cttcccagca aaaacaggac
    10861 tggctgttcg tgaccgaact caagcagcaa tacaccgtgg tgtccgtacc gctggatgcc
    10921 aaggaaattg ccgacgacat tgataccctg ctggtcattc accccaaggg catcagccag
    10981 cccacgctgt ttgcgctgga tcagtttgtg ctgcgcggcg ggcatctggt ggcctatctg
    11041 gatccgcagt gcatcgcgga tgcgccgtcc gacgaaatgc gcggaatgcc gccgccatcg
    11101 tcggacatca atggcctggc ccaggcgtgg ggcgtgacgg tggatgtctc ccgcgtggtg
    11161 gcggatgtcg ccgcggccac gccgatcaac atcggcgaag ggcgtgcgga gcgcctgccc
    11221 gcgtggctga ctttgcgcgg cgcggagaat cttaacccgg cggaaatcac caccggctcg
    11281 ctggaaagcc tgatggtgcc gtttgccggt gtgatccaag gcaaaccagc ggaagggctg
    11341 caaatgacca cgctggtgac cgcctccagc aacgcggtta cgctgaatct ttatcaggcg
    11401 cgcaacgcgc tggatgccag cacccgcgat ggcatcccgg cgccgaatgc cgccatcgcg
    11461 gtgcaactga cgggcaactt caccactgcc ttcccggatg gcccgcctgc ggcggagggc
    11521 gaggaagccg atgccgacaa gcccgccgac accaacacgt ggctcaaggc cagcgccgcc
    11581 cccggcgcgg tcgtcatggt ggccgatgcc gatatcctgg tgaccgactt catggcccgc
    11641 ggcatcaatt tcttcggccg gacgctctac cagccgttca acgacaacct caacttcacg
    11701 ctgaacatcg tggagcagat gtccggcagc ccggcgttga tcggtctgcg tggtcgcggg
    11761 aagtttgatc atccgtttga tcgcgtgctc gcgttggaga aggccgcgca ggagcaatgg
    11821 caggcgcagg aggagcagtt gcaacaaaag ctcatggaaa cccagcagcg gctcaacgaa
    11881 ctgcaagccg ccaagagcga cgaccagcaa ctggtcctga gcgcggagca aaaggccgag
    11941 ctggaaaaat tccggcagca gcgttttgaa acccagcgtc agttaaagga tgtccgcaag
    12001 aacctgcggc acagcattga gtcgttggga ctgcggctga aagtcattaa cctgatggcc
    12061 gtgcctctcg tggtggcggt cttcggggta gtgttgggct ggcggcggcg gcggcgcgcg
    12121 gtggcatagg caagggaggt tcacatgtcc aagaaatcat tatgggtttt ggtgttggtg
    12181 ctggtggcgc ttggcgcgct gctgtggtgg cagggtcggc aggaggctca gccggtgacg
    12241 gcttccgcgc aggcggccac ggccccgctg ctggccggcg tggattggaa cggcctgact
    12301 accatcatgc tggcggatgg cgtggccacc acgcatctgg cgaaagtgga tggcaactgg
    12361 acggtggtgg agcaggacgg ctatcctgcc gatgtgaacc gcctgcgcca gatgctgcgt
    12421 tcgctggatg atctggccgc cggtgaagtt gtcgcggccg ctacggatga cttgaccgag
    12481 tttgggttgg taccggcggg cgattctccg cccacgcgcc tccttttgga gcaagacggc
    12541 cggggttcca cgctttggct gggcaacatg cgcgcgccca agaacgacat gcaaatgtgg
    12601 atgcgcgaat ctggccgcta tgtgcgcgtg aatgatggtc ccgtgcgcct gatcaaggac
    12661 agcatggccc ggatcagcac cacacccgac gagtggtggg agcggcagat cgtggccgtg
    12721 gagccggcgg atgtggatga agtggcggtc acggcgggcg atgagtctta taccatcgcg
    12781 cgtgacgaca ccggcgttta cgcggtgcgc gacggcgcgg atggcgagac cgtggacgag
    12841 gccgccgccg aaagactgtt cgggttgcta cgcgatttac gcgcggacac cctcttgagc
    12901 gccgaacgcg ccgccgaact ggagtttgct cccgtggcga cttatcgcgc ggaaaccgcc
    12961 gctgccacct acaccgtgga aatcagcgag gcgttggccg atgaaaataa cggctggccg
    13021 ctgcgtctga ccgtgacagc ccccaatggc gagccgccgg ccgcgcataa atccgccggc
    13081 cggacgtatc tggttgcgcc gtacatcgcc gaccagttca aaactccgcg cggcgtgctg
    13141 gtcaaagccg cgcccgaacc ggaagaagcg gcagaggccg ccgccgggga agagcccgct
    13201 gaaaatgcgg aggaatccgc cgatgaaact cccgcggaga tcgcgccgcc acctgtgccg
    13261 gatgcggccg cggaggagcc cgccacgccg gcggaatccg ccgcggaggc cgcgcctgaa
    13321 tagcctgtgg cgcggcgagg gttctggttg tcaatgcgcc ggccggctgg catgatttgc
    13381 ggccatgtca accaaacggc aacacgaaat cggctgcccc ttttgtgggc gggagcaaac
    13441 ggcggaattg tgggacgcga ttgatgccgg ccaggatccc gagctgcgca ccgcgctgct
    13501 ggccggccag atcaaccgcg tgacgtgcgc cggctgcggc aagagtttcc gcatcgaaaa
    13561 ggcgctggtc tatcacgacc gcgagcagga gatttttgtg cattacgagc cgctggccgg
    13621 cggcattacc cagccacagg cagagcagaa tttccgtgcg gcgttgcagg agctggcccg
    13681 cctattgccc aaggacatgc ccgagtggga aacgcacctg gtgctgacgt ggaacgagct
    13741 gatcgagagg atttatctgc ttgaagaagg gctcgacgcg cggctggtgg agcacatcaa
    13801 atacatgatg taccaacaaa atccggccaa gctgccggcg gccaataaaa acctgttgtt
    13861 caacgcgcag gattccaccg acgatcagtt gtgcttcgtg gtgcaggacc gccagacgca
    13921 gaaactggaa gccgtgctga acttcgctcg cgcggactac gaggcgctgg tgaatgtgtt
    13981 tgattccgac gagcaattgg cgctgctgga agaacaattc ccggggcctt actacaacgg
    14041 acggcagcga tttttgcgtg atcaattgga cgcggaggat tgaacatggc cacggaatct
    14101 ttggcccaga aacaacgccg cgcccgcact attctgcgta aattgcgcca aacctatccc
    14161 gatgccaagt gcgagctgga ctggtccacg cctatcgagc tgaccgtggc cacgattctc
    14221 tccgcgcaat gcacggaccg gcgcgtgaac atggtcacgc ccgcgctgtt caaaaaatat
    14281 cgcaccgcgt gtgactgggc tgccaccccg gcggcaactc tggaagatga aatccgctcc
    14341 accggatttt tccgcaacaa gaccaaaaat atccgcgcac tgatggcccg gctggatcag
    14401 gaatttggcg gccaattacc cgacgatttc gaaacgctgg ttaagttgcc cggcattggc
    14461 cgcaagacag ccaacctgct ggtggccact gtgttcggca agccgggctt ggtggtggat
    14521 acgcacttta tccggctctc caaccggctg gggttcgtga aaaacgaaac cgacgccgtc
    14581 aaaattgaat atgcgttgca aaaaatcgtg ccatctgcgg agtggaccaa ctggtcgcat
    14641 gtaatggtgt tccacgggcg gcgctgctgc tacgcgcgca agccggaatg cgcccgctgt
    14701 ccgattcaag cgcagtgccc ggcggccaag acgttcctgg ccgcggcgac ctgaggcgca
    14761 tggaaaaccg caacgtcatc cggttcgcat ttgccgtggg gggcttcacc atgctgagcc
    14821 gggtgctcgg catggtgcgc gacgtgctga ccgcccggat gttcggcacg ctgccggtga
    14881 tgtcggcgtt tgtggtggcg ttccggctgc ccaacttgtt ccgcgcgttg ttcggtgagg
    14941 gtgcgctctc gtcggcgttc attccggtgt tcatggagtc gcgccggacg gaaggcgagg
    15001 cgccggcgtg gcgtttggcg cgccgggtca gcagtttggt cggggcggtg ctgttgggca
    15061 tcattgtggt gggcatcgcg gggatggggt tggcccggca ctgggtgacg gtgggcagca
    15121 aagcagcaaa cgttctgccg ctggcgcaaa tcatgctgcc gtatgtgttt ttcatttgca
    15181 tggcggcatt ggccatggcg gtgctgaatt cataccgcaa gtattcggtc tcggccttta
    15241 cgcccgccct gctcaacatc acgtggattt tgtccatcct gttcattgtg ccgctggtgc
    15301 gcgggggcac gtccatgcaa atccgcgcgc tggcgtggac ggtgtttgtg gccggcgcgg
    15361 tgcaactggt gtatcaggtg ccgtcgctgc tgcgcgtggg ttggcggccg ggcgtggata
    15421 cggactggcg ggacccgcgc gtcaaacaag tctttttgct gatggggccg gcggcgctgg
    15481 gcctggcggt caaccagatc aacgtgatga tcaacagtat gatcgccctg tgggccggcg
    15541 actgggcgcc ctccgcactt ttttatgccg agcggttgat ttatctgccg cagggcatcc
    15601 tcgccacttc gctgggcacg gtgttgctgc cggtgttgtc cgattttgcg gtgcagaaac
    15661 ggcacgacga catgcgcgcg gccatccacc acggcttgcg cacgttgctg ttcgtcatga
    15721 cccccgcggc ggtggggctc tttgtgctgg cggggccgat cgtgcaaatg ctgtttgagc
    15781 agggcgcctt tgatgcattc agcaccgtgc tgaccgcacg cgcgctggcg ttctacgcgc
    15841 ccggcctgat gctcttttgt ctggccaagg tgtttgtgcc gtctttttac gcgctgcaag
    15901 acacccgtac gccagtcaag gtgggattgt gcgcggtgac gctgaacctg acgctcaacg
    15961 ccatcttcgc gctgacgctg cccgaatact ggcggcacgc gggaatggcg ctggccacgg
    16021 gcatcgcgga aggtttcaac ggcctcacgc tggcgtggtt gctacggcgt cgtttggggc
    16081 cttttggcgt gcgcggcatt ttgggcggcg tggggcgcgc cctgctggcg gcgggggcca
    16141 tggcgctggt ggcgtggcag gtgcagcgcg tcggtacaac gtggctgtat ctgcacgtgt
    16201 cgcccaaaac ggcgcaactg atcggcgtgc cggcggccat cgcgctgggc gtcgggatat
    16261 atttcctgat cgcgcgattc gcgggttttc cggagttggg gtttgtgctg gaagcgctgc
    16321 gtgcccgccg gcaagaacgc gcggcggctc aatagccgct gatttcaacg cagggggggg
    16381 gagggagggg ctcagtccca cgtggggggc tggccggtgg tggcaatgac tgcgcgccac
    16441 aaatcgcctt cggggtccac cttcttgcgg ctggctgtgg cgagggccac cggcacgtgg
    16501 acatagacgc tgtaccagtc gccaatgatc aggttggtct tgccggccat ggcggcgtgc
    16561 accgcgttgc gcgcgaagtc gtcgcacagc cgcgcgtcct gcgagttggc ccgcacggag
    16621 cggatgacgt aggacggttc catgtacttc agattgacct c
//
LOCUS       DAOPNP010000049        16513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4182_length_16513_cov_8.845729, whole genome shotgun sequence.
ACCESSION   DAOPNP010000049 DAOPNP010000000
VERSION     DAOPNP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16513
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4182_length_16513_cov_8.845729"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..1439
                     /locus_tag="PKI64_05955"
     CDS             348..1439
                     /locus_tag="PKI64_05955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOE00590.1"
                     /translation="MKKYLMVMCLAGVMGSVMVRGAPEPPISMNPYRGAIVVDANTGE
                     ALFEDNADRAGFPASMLKLMDLFIVLDGVKRGSVRLDDMVRVTPEVAAIGGSQVYLDP
                     RESFSVEDMLYALIIQSANDAALALALHVGGTREGFVRMMNDKARELGLSPITQFQSP
                     HGLPPAAGQRADITTPRDFAKLCVALLKEHPDALKYTSATFRVFRPEPRLFEMRTHNH
                     LLTSVPGCDGLKTGYFTAAGYSIAATVQRDGARVIAVVMGSVDRKTRDAKATELLAIG
                     MQKASRPAPQPVVKPAPPPPAPAVVDAVEDDPLQGEDLPDATAEDAAPPTPEKARGGW
                     WKNVGLVLLGAFLATVVGMVVQRRLLLGR"
     gene            1436..2488
                     /gene="queA"
                     /locus_tag="PKI64_05960"
     CDS             1436..2488
                     /gene="queA"
                     /locus_tag="PKI64_05960"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOE00591.1"
                     /translation="MKVADFDYDLPPELIAQAPAARRDGARMMVLDRAARTIQHRTFA
                     ELPDFLRPADLLVVNDTRVIPARIFGRKAKPGTGGRVEFLLLEEVASGVWDALMRSRR
                     RPAPGDQVILDDDLAVVTLLAEGELGRVQIRVESALPWLEVLERIGQTPLPPYIARKE
                     TTPVQRAADKERYQTVFARDPGAVAAPTAGLHFTPEVLQRLAAMGVAQAAVTLHVGLG
                     TFRPVTTENVRDHKMDFERWWIPTETAAKVQAAKAGGGRVVAVGTTSVRTLESAAARP
                     EGFGAGQGRTELFIYPPYDFKVVDALLTNFHLPKSTLIMMISAFAGYGFVREAYRQAI
                     EERYRFYSYGDCMLIL"
     gene            2517..2888
                     /locus_tag="PKI64_05965"
     CDS             2517..2888
                     /locus_tag="PKI64_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOE00592.1"
                     /translation="MALTLEQMALAEVARLRAQLPPPIARLAAAVPVVAMPRPSQAMI
                     RDDGLEPDLLGLFVGASRAESVEGGDPLPPQILLFVDNIWDFAEGDEASFREEVRVTF
                     FHELGHYLGLAEGDLEIRGLE"
     gene            complement(2962..3873)
                     /locus_tag="PKI64_05970"
     CDS             complement(2962..3873)
                     /locus_tag="PKI64_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOE00593.1"
                     /translation="MKVTHFIRAAAMAGLLFTLAAGCTTTDFVTGQPVQNMYSLAEEV
                     QLGGQFYKELVSGLQKENAPIDQDPARVALVREVTAHITAVAHITNLPYHATFVGDPD
                     FVNAYALPGGNIMVFEGIWNPKEGLVRTVDELAAVIGHEIAHVNCRHSTEAMTRQMLP
                     NLLLAGGMIWATLEDKDDLQLLFGGLMLVHNGLIVTRYSRADELEADRIGMMYMAKAG
                     YNPEAAPRVWERLAGASDSQLAKALSIFSTHPRDKKRAQELRKHLPEAMALYQAAPIK
                     RDGARLLSTLPPSTTPAKPKSDKPKAD"
     gene            complement(4355..5431)
                     /locus_tag="PKI64_05975"
     CDS             complement(4355..5431)
                     /locus_tag="PKI64_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00594.1"
                     /translation="MMRVLLAGLSFDKVELHVAAALRRAGVQLFAINTPGTRAISGCQ
                     EHDIPFVTHEFRNRFDRAAIHLYRALHAEHSFDIIHALSNRALSTALWATRRLPAPPK
                     IVGYRGTMGHLHPLDPASRLSYLSPRVDRIICVSHAVQTYLQGLRIPAERLAVIWKGH
                     DPAWYTPAPRAALAECGIPPEATVAAFVGNIRPVKGVNVLLEAFRAIPPTEPFHLLLF
                     GEVRDRSIRRQIGRHPHIHFIGYRPDAAALAGACDIAVMPSIEREGLPKAILESMAQG
                     VPPIVTDVGGMPELVEHNHSGLVVPSRDAPALRAALLDLLHNPDRRRAMGQAARERIA
                     GPFHVRHTAEKTLALYHTLTTPSP"
     gene            5538..6689
                     /gene="tgt"
                     /locus_tag="PKI64_05980"
     CDS             5538..6689
                     /gene="tgt"
                     /locus_tag="PKI64_05980"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOE00595.1"
                     /translation="MAEIGTFEILHEDAGSQARLGRLWTAHGPVETPVFMPVGTQATV
                     KSMAPWELEAHGCEILLGNTYHLNVRPGMEVIEKLGGLHRFQDWKRAILTDSGGYQVF
                     SLTHLRRMTPEGVWFQSHVDGTKMFMGPKESMAIQRILGSDIAMAFDECPPCPCPPEY
                     ACQAVERTLSWAASCAQQPRAPGQLVFGIVQGGVDPRLRERCAEGLLALGAFDGYAIG
                     GVSVGEPEELIMPGVRMTAGLLPRARPRYLMGVGQFWQMAESVALGVDMFDCVMPTRL
                     ARHGTVFTRQGRYSVRAAVNRLDERPLEEGCTCVACQRFSRGYVRHLLNVGEILGVRL
                     TALHNMHCYFDFIARLRQSIRAGTFAAFRAETARVYGPDAPGPGAAAGA"
     gene            6712..7065
                     /gene="yajC"
                     /locus_tag="PKI64_05985"
     CDS             6712..7065
                     /gene="yajC"
                     /locus_tag="PKI64_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOE00596.1"
                     /translation="MNGWMMLAMMGAQTGGESAQPQSGAFMFIWLGLMIALFYFMLIR
                     PQKRREKERQALLSAVKTGDRVLFAGGFLGVVANVKDKTLVVKIADGVKVEIVRGAVS
                     QVLDKGETPDGAEQK"
     gene            7132..9567
                     /gene="secD"
                     /locus_tag="PKI64_05990"
     CDS             7132..9567
                     /gene="secD"
                     /locus_tag="PKI64_05990"
                     /inference="COORDINATES: protein motif:HMM:NF009583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOE00597.1"
                     /translation="MKASIWKWLILVVATAWSVALIAPPKEKVKLGLDLQGGTSYTLE
                     IDDTEGIVGGVSDARDRAVEVVRNRVDSMGVAEPTIYPDGDRRIIVQIPGMKAEDRER
                     ASANIRKAAYLEFRMVHPKNDDLIRNLFADRKVPEGYEMAVIPGRTQGDAWVRKGPPP
                     TEAERAALRRFEEKAGYDLLLEKVTEQGKDYFVPHYVSRKAELTGDSLKSANVDYQQF
                     GQRVVQISFDARGRKKFAQLTADHAPGGVKNPDPNGRHYLGIVLDNTLYSAPFIRTSI
                     PGGEAIIEGSFTLEEARDLALVLRAGALPAPLRVIEERSVDPTLGVDSIQSGWRAAGL
                     GFVAVALFMVVYYRFAGFVAVLALLFNLILLPLGMIITGGFFGLIGGAGGLGSSGVQL
                     PVLTLPGIAGIVLTVGMAVDANVLIFERIREEMRLGKRFGAAISAGYDKAFSTIFDSN
                     LTTLLTAVILFWMGSGPVKGFAVTLTAGIIASMYTAIVGTRMVFDFLEGHGYLNKLTM
                     TQWVKETKVDFMAKRWVAGIASLVIIAGSLAVAVHRGSANFGVDFTGGQQLTLDFVQK
                     AEVDTLRAALAGAGLTDVGIQYQRSGTTEKQNEVLALKVAGPEAGALAQQVLAEQFAD
                     SKFKLLQEDTVGPQVGRELQRSGLIAIGLSLVGMILYITIRFEFSFAVGAVAGLLHNL
                     LITIGLYCVFQRQFSTTTIAALLTVLGYSVNDTIVVFDRIRETRKLRAGRLDAGIVNE
                     SVNATLSRTLLTSLTTLLSVVMLLVFGGGAIFDFALALFIGVVAGTYSSIFIASPIML
                     AVRPRVALAPAGK"
     gene            9573..11309
                     /gene="recJ"
                     /locus_tag="PKI64_05995"
     CDS             9573..11309
                     /gene="recJ"
                     /locus_tag="PKI64_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOE00598.1"
                     /translation="MLIRAERNWVTPADANGVAAQLALAFNFTPAMGRALVARGLTDP
                     AEIERFLQPRLEDLTDPFELPGMAKAVDLILQHIEQGSSILVYGDYDVDGVTATALMI
                     EVLSALGAVATPFLPHRIEDGYGLGVETLHRCVEQYHPKLIITVDCGTSSVEAVEEAA
                     RLGIDVVVTDHHEPATEGVAAACAIVNPKLGNCPEHVRMLAGVGVAYKVCHGLITEAR
                     RRKMEGADSVDHHTFMDLVAIGTVADIVPLLQENRILVYHGLQLLNKTQSVGVRMLIE
                     AAGITGPVDTYHIGFIIGPRINAAGRLGKADTAFELLMTEQEPRARSLAQQLDEASRK
                     RQEIEDKIVSEACEDLDGRFDAERDFGLVVAHSNWHTGVIGIVASRIASRYNRPVVVI
                     GVDEDGVGRGSCRSVPGFDLLSHLQQCSPLLRRYGGHAMAAGLEVDKKSIPAFAEKFN
                     AVCGETLRQGDQRRQLLVDAWIQPHEIGDALYHDQNRLRPFGHSNPLPVWAVRNVTPR
                     FAPRVIGGKHLKLIFPMAGKDREAIGFGMGDREVPPGPLDIAFNLILNRFKGTEYLQL
                     HLQDFRPSQARE"
     gene            11690..11950
                     /locus_tag="PKI64_06000"
     CDS             11690..11950
                     /locus_tag="PKI64_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOE00599.1"
                     /translation="MSEPQKPFRVPYPAIDPLECKACGRCIAACPKKVIRMGTQLNAR
                     GYTFAEYVGDGCIGCLNCFYVCPEPNAIAVHVPDRRAPKQES"
     gene            11955..13013
                     /locus_tag="PKI64_06005"
     CDS             11955..13013
                     /locus_tag="PKI64_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOE00600.1"
                     /translation="MATQLVKGNTAVVIGALYAGCDSFFGYPITPASEILHEAAAKFP
                     LAGRRVVQAESEEAAINMVYGAGATGRRTMTASSGPGISLKQEGISYLAGAQVPAVVV
                     DIMRAGPGLGNIGPEQGDYNQVVKGGGHGNYRNLVLAPASVQEMCDFTMLAFELAHKW
                     RMVAFVLADGVLGQMIEPLRFPETSVHPQTDTSWAVDGTAATRPNVVTSIFLNFDKQE
                     AFNRQLQDKYAAVEAAEARHEAYLLEDADIALVAYGISARIALGAVDAARAEGLRVGL
                     FRPQTLYPFPAAALQEFVARGGKQLISVEMSNGQMRDDIKLAIECRCPVHLVSRLGGN
                     LVTAAEILERVRAIAQPA"
     gene            13030..14457
                     /locus_tag="PKI64_06010"
     CDS             13030..14457
                     /locus_tag="PKI64_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOE00601.1"
                     /translation="MEKVIETRGLYKVFPRKGDSALTATHYCPGCEHGVLHKLIGEAL
                     ADLNLQDRTVMISPVGCAVFGYYYFDTGNIQVAHGRAAAVATGLSRTMNQAVVGAYQG
                     DGDLASIGLNETLQAANRGEKLAVFFVNNSVYGMTGGQMAPTTLVGEKTETSPEGRDP
                     LQMGYPLHMAELINQLQAPVYIARVSFSNMAKLRQGRQAIRKALEIQRDGKGYAFVEV
                     LMACPTNQKMNVEAATRFVAEQMEAEYPLGIFRDRSAEAQPIVRSESDYRKETLDRLL
                     GLDTDQAPDAQLDPAAPKWKIKMAGFGGQGVLSMGVILARAACRAGKFVSWYPSYGPE
                     QRGGTANCGVVFAGQPVGSPMVTEADVLVAMNRPSLEKFAAEVKPGGWLLYNAAIGDF
                     TPPAGVRALAVPANELAQEGGNPRGANTAMLGVLAELGGFGLADEHYRAAIAANFAGR
                     EKIIQQNQAVFAHARAWAAKQQIPH"
     gene            complement(14454..14621)
                     /locus_tag="PKI64_06015"
     CDS             complement(14454..14621)
                     /locus_tag="PKI64_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00602.1"
                     /translation="MADAIADFSAIRGGKVSFLNHSRPTKAAGWFVGLERSQNFQPFP
                     ARKKIRVMPVG"
     gene            14718..15494
                     /locus_tag="PKI64_06020"
     CDS             14718..15494
                     /locus_tag="PKI64_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOE00603.1"
                     /translation="MSYQVIWAWVNAPGLDQPVERIAFINGEARQRQVFHADGLGSIA
                     ALTDESGATIQTYAYEAFGSIRTQTGTDLNRVTFTAREAMGDTLGFFYYRHRIYDPHT
                     GRFTSEDPLGFAADPNRYVYVINSPVNMTDALGMEGEGFDSDPIIKDFFDKLSQDAEW
                     FSENEHEEGGHVNQCITNGEVTPVQWPPGTTYRIPVPDQYHIDKSNPNIIVLLTVHTH
                     PPSEPKPSDQDKTNANARGVPLVILNFLENNTYYPPPCDN"
     gene            15507..16088
                     /locus_tag="PKI64_06025"
     CDS             15507..16088
                     /locus_tag="PKI64_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00604.1"
                     /translation="MRAKYDLLIVCFGLCCLSQLLESHAGYIEVIWENYPAPYISSLY
                     VNSSGEYAYRTKYDDTKVERNNIARILNMAGSNSAYSIKIKANIDTYVADNIEHVSYE
                     RSLSQLVEYYRNNSNIIVAKPGDINIEDGWKIQIIFYDDVEIYSVIFRDNPDIHCARM
                     LSIIQEIGSVFAEYLSTPLSWAVHGVEIFPERE"
     gene            16276..>16513
                     /locus_tag="PKI64_06030"
     CDS             16276..>16513
                     /locus_tag="PKI64_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00605.1"
                     /translation="MLGGLPRDEAGQPVVFDGAGNRISQSLNDCLTTRFVYDGPNVVM
                     EMSAGGGSAYGGNVSHQVIWAWVNAPGLDQPVERI"
BASE COUNT         3302 a         4595 c         5471 g         3145 t
ORIGIN      
        1 gtcgtattct gacggcagcg cggattttgt ttgcgcaaat aaaatcagtc acctcaaatt
       61 catccagagt ttataaactc tggatggtgc cttcaaaaca gccgatcaaa aatagcctaa
      121 aaaatataca taacagattg tatatgatct tgatatgaat attacaaaaa tcatccacac
      181 tttataaact ctggatgaca tggttcaaat ggcaaaaaca aagaggttgg tttgacgagg
      241 ggagttcact catcccaagc gggacttttc agccgctcaa aacaacggga gacttgaaaa
      301 tcagggttgg tcttgaattt cggaaggggg atggctacaa ggggcgcatg aaaaaatatc
      361 tgatggttat gtgtttggcg ggggtgatgg gctcggtgat ggtgcggggc gcgccggaac
      421 cgccgatttc aatgaatcca taccggggcg cgattgtggt ggatgcgaat accggggaag
      481 cgctgttcga ggataatgcc gaccgcgcag ggttccccgc cagtatgctt aaattgatgg
      541 atttgttcat cgttttggat ggtgtcaaac gaggcagtgt gcggctggat gacatggtgc
      601 gggtgactcc ggaagtcgcg gccatcggcg gttcgcaggt ctatttggac ccgcgtgagt
      661 cattcagcgt ggaggatatg ctctacgccc tgatcattca gtcggcgaat gacgcggcgc
      721 tggcgctagc actgcatgtg ggcggcacgc gcgagggatt cgtgcggatg atgaatgaca
      781 aggcgcgcga gctgggcctt tcgcccatca cccagttcca gtcgccacac ggtttgccgc
      841 cggcggcggg gcagcgtgct gacattacga ccccgcgtga cttcgccaaa ctgtgtgtgg
      901 cgctgctcaa ggagcacccc gatgcgctca agtacacctc ggccacgttt cgggtttttc
      961 gccccgagcc ccggttgttc gagatgcgca cgcataacca tttgctgacg agcgtgccgg
     1021 gctgcgatgg actcaaaaca ggctatttca cagcagcggg ctactcgatt gccgcgacgg
     1081 tgcagcgcga tggcgcgcgc gtcattgcgg tggtgatggg cagcgtggac cgcaagactc
     1141 gcgatgccaa ggcaacggag ctgttggcca tcggaatgca gaaggcttcg cggcccgcac
     1201 cacagccggt ggtgaagcct gcgccacccc cgcctgcgcc ggcagtggtg gatgccgttg
     1261 aggatgatcc gctacaaggc gaagacttgc cggacgccac cgctgaagat gccgcgccgc
     1321 ccacgccaga aaaagcccgc ggcggctggt ggaaaaacgt ggggctggtg ttgctgggcg
     1381 cctttttggc aacggtggtc ggcatggtgg tgcagcgccg cctgctgctg ggccgatgaa
     1441 ggttgcggat tttgactacg acctgccgcc ggagctaatt gcgcaggcac cggccgcgcg
     1501 ccgcgacggc gcgcgcatga tggtgctgga tcgagcggcc cgcaccattc aacaccgcac
     1561 ctttgccgag ctgccggatt ttttgcggcc cgccgacttg ctggtggtga acgatacccg
     1621 cgtgatcccc gcgcggattt ttggccggaa ggccaagccg ggcactggcg ggcgcgtgga
     1681 gtttttgctg ctggaggagg ttgcctccgg ggtgtgggac gcgctgatgc gttcgcgtcg
     1741 ccgcccggcg ccgggcgacc aagtgattct ggatgacgac ctggccgtgg tgacgctgct
     1801 ggcggaggga gaactggggc gggtgcaaat ccgggtggag tcagctttgc catggttgga
     1861 agtgttggaa cggatcgggc aaacgccgct cccgccatac atcgcccgca aagagacaac
     1921 gcccgtccag cgcgctgcgg ataaggaacg ctaccaaacc gtattcgcgc gcgatccggg
     1981 ggcggtggca gcgccgacgg cggggctgca tttcaccccg gaggtgctgc aacggcttgc
     2041 cgccatgggc gtggcgcagg cggcggtgac cctgcacgtg gggctgggca cgtttcgccc
     2101 cgtcaccacg gaaaacgtgc gtgaccacaa aatggatttt gaacgttggt ggatccccac
     2161 ggaaaccgcg gcaaaagtgc aggccgccaa agccgggggg gggcgtgtgg tggcggtggg
     2221 caccaccagc gtgcgcacgc tggaaagcgc ggccgcccgc ccggagggct tcggcgccgg
     2281 ccaaggacgg accgaattat ttatttatcc gccttacgac ttcaaagtcg tggatgcgct
     2341 cctgacgaat ttccacctgc caaaatccac gttgatcatg atgatcagcg cgtttgccgg
     2401 ctatggtttc gtgcgcgagg cctatcgcca agccatcgaa gagcgctacc ggttttatag
     2461 ctatggcgac tgcatgttga tcctgtgaac gcgacgcggc aaaggcggcg aaacaaatgg
     2521 ccctgacctt ggaacaaatg gcgttggccg aagtggcccg gctgcgcgcg caactgccgc
     2581 cgcccattgc gcggttggcc gccgccgtgc cggtggtggc catgccgcgc ccctcgcagg
     2641 cgatgatccg cgacgatggc ttggagccgg acttgctagg gttgtttgtc ggcgccagcc
     2701 gggcggagag cgtcgagggt ggcgatccgt tgccaccgca aatcctgctg tttgtggaca
     2761 acatctggga ttttgcggaa ggcgacgaag cgagctttcg cgaagaagtg cgcgtgacgt
     2821 ttttccacga gctgggtcac tacctcggct tggcggaagg cgatctcgaa atccgcggct
     2881 tggaataggc gtaacagtcc atgcacgaca gtgcgctggg cattgtagcg gccagcgcgg
     2941 ggggggaggg gcagtttacg gttagtcggc cttgggtttg tcggattttg gtttggccgg
     3001 cgtggtggag ggcggcaggg tggaaagcag ccgggcaccg tcgcgtttga tgggggcagc
     3061 ttgatacaac gccatggcct ccggcagatg tttgcgcagt tcctgggctc gttttttgtc
     3121 gcgcggatgc gtggagaaaa tgctcaacgc cttggccaat tggctgtcgc tggcgccggc
     3181 gagccgctcc cagacgcgtg gcgcagcctc ggggttatag ccggccttgg ccatgtacat
     3241 catgccgatg cggtcggcct cgagctcatc cgctcgggag tagcgggtaa cgatcaaccc
     3301 gttgtggacg agcatcaacc cgccaaagag cagttgcagg tcgtccttgt cctccagcgt
     3361 ggcccaaatc atgccgccgg ccagcagcag gttcggcagc atttggcggg tcatggcctc
     3421 ggtggaatga cggcaattga cgtgcgcaat ttcgtggccg atgactgccg ccagttcatc
     3481 cacggtgcgc acgagaccct ccttgggatt ccagatgccc tcaaacacca tgatgttgcc
     3541 gccgggcagc gcgtaggcgt tgacgaaatc cgggtcgccc acgaaggttg cgtggtaggg
     3601 caaattggta atatgcgcca cggcggtgat gtgcgccgta acctcacgga caagggccac
     3661 gcgggcgggg tcctgatcaa tcggtgcgtt ttctttttgg agaccgctca ccagctcttt
     3721 gtaaaactgg ccaccgagtt gcacttcctc cgccagggaa tacatattct gcaccggctg
     3781 accggtgaca aaatccgtgg tggtgcaccc ggccgccagc gtaaacaaca agccggccat
     3841 cgccgccgcg cggataaaat gggtaacctt cattgcatca tctccagcct tttcgtcgtc
     3901 gccaattcgc cgagtggggt tctgcatgcc aagccccaaa ctctatcaca ccactctgga
     3961 aaaatgccgt cgtgaagtcc agcgaatcga gaccgttttg gggcaggcac taacaatgtc
     4021 aacattggga tatttgccga ttttttgtgg ggaggcaact gccatacggg gtctcaccgc
     4081 gcggataaaa acacaaaaat cagtcacctt aaaattgtcc agagtttata aactgtggac
     4141 attgctctca aaatggccga ttaaaaataa ccagaaaaat aatcataaca agttgtatat
     4201 gatttagata tgaatattac aacaattgtc cagactttat aaactctgga cagtgtggcc
     4261 aaatggcaaa aaaacagatg ccgaattttt ctgcagcgcg tggggcaaga atgttgattg
     4321 ttaagcgctg accccaaacc tctttccgga gccgtcaagg gctgggggtg gtgagggtgt
     4381 ggtatagggc aagagttttt tcggcggtgt ggcggacgtg gaaggggccg gcaatccgtt
     4441 cgcgagcggc ttggcccatg gcgcggcggc ggtcggggtt atgcagtaga tcaaggaggg
     4501 cagcgcgcaa ggcgggggcg tcgcgcgaag ggaccacaag gccggagtgg ttgtgctcca
     4561 ccagttcggg catgccgccg acatcggtga cgatgggggg cacgccttga gccatgcttt
     4621 ccaagatggc cttggggagg ccttcgcgtt cgatggaggg catgaccgca atatcgcacg
     4681 caccggcaag ggcggcggca tcaggacggt agccaataaa atggatatga gggtgccggc
     4741 cgatttgccg gcggatggag cggtcgcgca cttcgccaaa cagcagcagg tgaaaaggtt
     4801 cggtgggggg gatggcgcgg aaagcctcca acagaacatt cacccctttg acggggcgga
     4861 tattgccgac aaaggccgca accgtggcct ccggcggaat gccgcattcc gccagcgcgg
     4921 cgcggggggc gggggtgtac cacgccgggt cgtggccttt ccaaatcacg gcgaggcgtt
     4981 cggcggggat acggagtcct tgcagatagg tttgcaccgc gtgggaaacg caaatgatgc
     5041 ggtccacgcg cggagataaa taggaaaggc gtgaggcggg gtccaaaggg tgcagatggc
     5101 ccatggtgcc acggtagccg acgattttgg gcggagcggg gaggcggcgg gtggcccaca
     5161 gggcggtgga gagggcgcgg ttgctgagtg cgtggatgat atcaaatgaa tgctccgcgt
     5221 gcagggcgcg gtagaggtgg atggcggcgc gatcgaagcg gttgcggaat tcgtgggtga
     5281 cgaacgggat gtcgtgctcc tgacaccccg agatggcgcg ggtgccgggg gtgttgatgg
     5341 cgaatagttg cacgccggcg cgccgcaggg cggcggccac gtgcagctca accttgtcga
     5401 aactcaagcc ggctagaaga acgcgcatca tgttgattgt cccgcatgat agcagggcag
     5461 ggcggcttga cgaccttttg ttttggctgt ctctgccctc gacaggggcg ggggcggggg
     5521 gtagagtacc cgcctttatg gccgaaatcg gcacatttga aattttgcac gaggatgcgg
     5581 ggtcgcaggc ccggctgggg cggctgtgga ccgcgcacgg gccggtggag acgccggtat
     5641 tcatgccggt gggcacgcag gccacggtaa aatccatggc accgtgggag ctggaggcgc
     5701 acgggtgcga gattttgctg gggaacacgt atcacctgaa tgtgcggccg gggatggagg
     5761 tcattgaaaa acttggcggg ctgcaccggt tccaagattg gaaacgggcg attttgacgg
     5821 atagcggggg ttatcaggtg ttcagcttga cgcatttgcg acgaatgacg ccggaggggg
     5881 tttggtttca atcgcacgtg gacggcacca agatgttcat ggggccgaag gaaagcatgg
     5941 cgattcagcg cattctgggg tcggacatcg ccatggcgtt tgacgagtgt ccgccgtgcc
     6001 cttgtccgcc ggaatacgct tgccaagcgg tggagcgcac cctatcgtgg gcggccagtt
     6061 gtgcccaaca gccccgggct ccggggcagt tggttttcgg cattgttcag ggcggcgtgg
     6121 acccgcggtt gcgggagcgc tgtgccgagg gcttgctggc gctgggcgcg ttcgatggct
     6181 acgccatcgg cggcgtgagc gtgggcgaac cggaggagct gatcatgccg ggcgtgcgga
     6241 tgaccgcggg cttgctgccc cgggcgcgcc cgcgctacct gatgggcgtt ggccagttct
     6301 ggcaaatggc cgagtcggtg gcgctgggcg tggacatgtt cgactgcgtg atgccgacgc
     6361 ggctggcgcg gcacgggaca gtgttcaccc gccagggccg ctactcggtc cgggcggcgg
     6421 tgaaccgcct cgatgagcgc ccgctggagg aaggctgcac gtgcgtggcg tgccagcggt
     6481 tcagccgcgg gtatgtgcgg cacctgctca acgtgggcga aatcctgggc gtgcggctga
     6541 cggcgctgca caacatgcac tgctattttg attttattgc ccggctgcgg cagagcatcc
     6601 gggcgggaac gttcgcggcg ttccgcgcgg aaacggcccg cgtttatggg ccggacgcgc
     6661 cgggaccggg cgccgcggcg ggagcctgat ggcaaagagg agaactgaaa aatgaatggt
     6721 tggatgatgt tggccatgat gggcgcccag acaggcggcg agagtgcgca gccgcaatcg
     6781 ggcgcgttca tgtttatttg gctgggactg atgattgccc tgttttattt catgctgatc
     6841 cgcccgcaga agcgccgcga gaaagagcgg caggcgctgt tgagcgcggt gaagacgggc
     6901 gaccgcgtgc tgtttgccgg gggcttcctg ggcgtggtgg cgaacgtgaa ggacaagacg
     6961 ctggtggtga agattgccga tggcgtcaaa gtggaaatcg tgcgcggcgc ggtgtcgcaa
     7021 gtgctggaca agggcgaaac gccggacggc gcggagcaga aataggcgcg gggagcgcca
     7081 cggatttggc cggcggctgc cgccggcgca gtggagaaaa cgcgaggacg tatgaaggca
     7141 tcaatctgga aatggctgat tttggtggtg gcaaccgcat ggagcgtggc gctgatcgcg
     7201 ccgcccaagg agaaagtcaa gctggggctc gacctgcagg gcggcaccag ctacacgctg
     7261 gaaattgacg acaccgaagg catcgtgggc ggcgtgagcg acgcgcgcga ccgcgcggtg
     7321 gaagtggtgc gcaaccgcgt ggactccatg ggcgtggccg agccgaccat ttatcccgac
     7381 ggcgaccggc gtatcattgt gcagattccg ggcatgaagg ccgaggaccg cgagcgcgcc
     7441 tccgccaaca tccgcaaggc ggcgtatctg gaattccgca tggtgcaccc gaagaacgac
     7501 gacctgatcc gcaacctgtt cgcggaccgc aaggtgccgg aaggctacga aatggccgtg
     7561 atccccggcc gcacgcaggg cgatgcctgg gtgcgcaagg ggccgccgcc gaccgaagcc
     7621 gagcgcgccg cgctgcgccg ctttgaggaa aaggcgggct atgacctgct gttggaaaaa
     7681 gtgaccgaac agggcaagga ctactttgtg ccgcattatg tcagccgcaa ggcggagctg
     7741 acgggcgact cgctcaagag cgccaacgtg gactaccagc aatttggcca gcgcgtggtg
     7801 cagatttcgt tcgatgcgcg cgggcgcaag aagttcgcgc aactgacggc ggaccatgcg
     7861 ccgggcggcg tcaagaatcc cgaccccaac gggcggcatt atctgggcat tgtactggac
     7921 aacacgctct attccgcgcc gttcatccgc acttcgattc ccggcgggga agccatcatt
     7981 gaaggcagtt tcaccctgga agaagcgcgc gacctcgcgc tggtgctgcg cgccggcgcg
     8041 ctgccggccc cgctgcgggt gatcgaagaa cgcagcgtgg accccacgct gggcgtggac
     8101 tccatccaaa gcggctggcg ggcggccggg ctgggctttg tggccgtggc gctgttcatg
     8161 gtggtctatt accgctttgc cggttttgtg gccgtgctgg cactgctgtt caacttgatc
     8221 ctgctgccgc tgggcatgat catcacgggc ggcttcttcg gcctgattgg cggggcgggg
     8281 gggctgggca gttccggcgt gcagttgccg gtactgacgc tgccgggcat tgccggtatt
     8341 gtgctgaccg tcggcatggc ggtggatgcc aacgtgctga tctttgagcg catccgcgaa
     8401 gaaatgcgcc tgggcaaacg cttcggagcc gccatttccg cgggctatga caaggcgttt
     8461 tcaaccatct tcgactccaa tctgaccacg ctgctgacgg cagtcatcct cttctggatg
     8521 ggctccggcc cggtgaaggg gtttgcggtc acgctgacgg cgggcatcat tgccagcatg
     8581 tatacggcca ttgtcggcac gcgcatggtg ttcgacttcc tggaaggcca cggctacctg
     8641 aacaagctga ccatgaccca gtgggtcaag gaaaccaagg tggacttcat ggccaagcgg
     8701 tgggtggcgg gcattgcctc gctggtgatc attgccggtt cgctggcggt ggcggtgcat
     8761 cgcggcagcg ccaacttcgg cgtggacttc acgggcggcc agcagctcac gctggacttc
     8821 gtgcagaagg ccgaagtgga taccctgcgc gcggcgctgg ccggcgcggg cctgacggat
     8881 gtgggcatcc agtatcagcg cagcggcacc accgagaagc aaaacgaagt gctggcgctg
     8941 aaggtcgccg ggccggaagc cggcgcgctg gcgcagcagg tgctggccga gcagtttgcc
     9001 gacagcaaat tcaagctgct gcaggaagac accgtgggcc cgcaggtggg ccgcgagttg
     9061 cagcgcagcg gcctgattgc catcgggctc tcgctggtgg gcatgatcct ctacatcacc
     9121 atccgcttcg agttttcgtt tgcggtgggc gcggtggcgg ggctgctgca caacctgctg
     9181 atcaccatcg gcctctactg cgtattccag cgccagttct ccaccaccac cattgccgcg
     9241 ctgctgacgg tgctgggtta ctcggtcaac gacaccatcg tggtctttga ccgcattcgc
     9301 gaaacgcgca agctgcgcgc cgggcggctg gatgcgggca tcgtcaacga gagcgtgaac
     9361 gccacgctgt cgcgcacgct gctcacctcg ctgactacgc tgctgagcgt ggtgatgctg
     9421 ctggtgttcg gcggcggcgc catcttcgac ttcgcgctgg cgctgttcat cggcgtggtg
     9481 gcgggcacct attcttcgat cttcatcgcc tcgccgatca tgctggcggt gcgtccgcgc
     9541 gtggcgctgg cgccggccgg gaagtaggcc cgatgctgat ccgcgcggaa cgcaattggg
     9601 tcacccccgc cgatgcaaac ggggtggcgg cccaactggc gctggcgttc aatttcacgc
     9661 cggccatggg ccgcgcgctg gtcgcgcgcg gcctgaccga tcccgcggaa atcgagcggt
     9721 tcttgcagcc gcgcctggaa gacctgaccg acccgttcga gctgcccggc atggccaagg
     9781 ccgtggacct gattctccag cacatcgagc agggcagctc catcctggtc tatggcgact
     9841 acgacgtgga cggcgtgacg gccaccgcgc tgatgattga agtcctcagc gcgctgggcg
     9901 cggtggccac gccgtttttg ccgcaccgca ttgaggatgg ttacggcctc ggcgtggaga
     9961 cgctgcaccg ctgcgtggaa caataccatc ccaaactcat cattactgtg gattgcggca
    10021 ccagctccgt tgaggccgtg gaagaagccg cgcggctggg cattgatgtg gtggtcaccg
    10081 accaccacga gcccgccacc gagggcgttg ccgccgcctg tgccattgtg aatcccaagc
    10141 tgggcaactg cccggagcac gtgcggatgc tggccggggt gggcgtggcc tacaaggttt
    10201 gccacgggtt gatcaccgaa gcgcgccgcc gcaagatgga gggcgccgac agcgtggatc
    10261 accacacgtt catggatttg gtggccattg gcaccgtggc ggacatcgtg cccctgctgc
    10321 aggaaaaccg cattctggtc taccacggct tgcagttgct gaacaaaacg cagtcggtgg
    10381 gcgttcgcat gctgatcgag gccgcgggca tcaccgggcc ggtggatacg tatcacatcg
    10441 ggttcatcat cgggccacgc atcaatgctg ccggacggct cggcaaagcc gacaccgcct
    10501 tcgagttgct aatgactgaa caggagccgc gcgcgcgcag ccttgcccag cagctcgacg
    10561 aagccagccg caagcggcag gaaatcgagg acaagatcgt cagcgaggcg tgcgaggatc
    10621 tcgacggacg ctttgacgcc gagcgggatt ttggcctggt ggtggcgcat tccaactggc
    10681 acacgggcgt gattggcatc gtcgcctcgc gcattgccag ccgctacaac cgccccgtgg
    10741 tcgtgattgg cgtggatgag gacggcgtcg gccgcggctc ctgccgcagc gtgccgggct
    10801 ttgatttgtt gagccacttg cagcagtgct cgcccctgtt gcgccgctat ggcggccacg
    10861 ccatggccgc cggcctcgaa gtggacaaaa aaagcattcc ggccttcgcg gaaaaattca
    10921 acgcggtttg cggtgaaacc ctgcggcagg gtgaccagcg ccggcaactg ctggtggatg
    10981 cgtggatcca gccgcacgaa atcggcgacg cgctctacca cgaccaaaac cgcctgcgtc
    11041 ccttcggcca ctccaatccc ctgccggtct gggccgtgcg caacgtcacc ccgcgctttg
    11101 ccccgcgcgt gatcggcggc aagcacctca aactgatttt cccgatggcc ggcaaggatc
    11161 gcgaagccat cggcttcggc atgggcgacc gcgaagtgcc gcccggcccc ctcgatattg
    11221 ccttcaacct gatcctcaac cgcttcaagg gcacggaata tctgcaactg cacttgcagg
    11281 acttccggcc cagccaggcc cgggaatgaa ccgcacggcc ggggccgccg cccaaatgtt
    11341 ttccacaccg tggaaaactt tgcatcccgc acgggcagga ttttccacac tgtggaaaaa
    11401 tcgggcgttt ttttccacac cgtggaaaac ttttttccac actgtggaaa aatcccgccg
    11461 cgccgccccc attttttcca cgcaatggga aaagtttgcg cgatttttcc acacaatgga
    11521 aaaactttgg gccaaaagtt ccacactgtg gaaaaatatg gcccgcctgc cgtttatttt
    11581 tccacaccgt ggaaaatccc gcccacgcgg aataaacttt tttccacagt gtggaaaagt
    11641 tcgggggatg gggtaggttg acctattggt gcagtgacat ggagccagca tgagcgaacc
    11701 acagaagccc ttccgggtgc cgtatccggc gattgatccg ctggagtgca aggcctgtgg
    11761 ccgctgtatt gcggcgtgcc ccaaaaaggt gatccgcatg ggcacgcagc tcaacgcgcg
    11821 gggctacacg tttgccgagt acgtgggcga cgggtgtatc ggctgcctga attgcttcta
    11881 tgtgtgcccg gagccgaatg ccatcgcggt gcatgtgccg gaccggcgcg cgcccaagca
    11941 ggagagttga aagcatggcc acgcaattgg tgaagggcaa cacggccgtc gtcatcggcg
    12001 cgctgtatgc ggggtgcgac agttttttcg gctatccaat cacgccggcc agcgaaatcc
    12061 tccacgaggc cgcggccaag ttcccgctgg cgggaaggcg cgtggtgcag gcggagagtg
    12121 aagaagcggc catcaacatg gtctatggcg cgggagccac ggggcggcgc accatgacgg
    12181 catcgtcggg gccggggatc agcctcaagc aggagggcat ttcctatttg gcgggggcgc
    12241 aggtgccggc ggtggtggtg gatatcatgc gcgccgggcc cgggctgggg aacatcgggc
    12301 ccgaacaggg cgactataac caagtggtga agggcggggg ccacggcaat taccgcaacc
    12361 ttgtgctggc gccggccagc gtgcaggaaa tgtgcgactt caccatgctg gccttcgagc
    12421 tggcccacaa gtggcgcatg gtggcctttg tgctggccga tggcgtgctg gggcagatga
    12481 tcgagccgct gcgcttcccg gaaacgagcg tccacccgca aacggatacg agctgggcgg
    12541 tggatggcac cgcggccacg cggccgaatg tggtgacctc catctttctc aatttcgaca
    12601 agcaggaggc gttcaaccgc cagttgcagg ataaatatgc ggcggtggag gcggccgagg
    12661 cgcggcatga ggcgtatctg ctggaggatg ccgatatcgc gctggtggcc tatggcatca
    12721 gcgcgcggat tgcgctgggc gccgtggatg cggcgcgcgc cgaggggctg cgcgtggggc
    12781 tcttccgccc ccaaacgctt tacccgttcc cggcggcggc gctgcaagag tttgtggcgc
    12841 gcggcggcaa gcagttgatt agcgtggaaa tgagcaacgg ccagatgcgc gatgacatca
    12901 aactggccat cgagtgccgc tgcccggtgc atttggtcag ccggctcggc ggcaatctgg
    12961 tgacggcggc ggaaatcctg gaacgggtgc gcgccatcgc gcagccggca tgaggcagcg
    13021 gagacagcca tggaaaaagt gatcgaaacc cgcggcctct acaaggtctt cccccgcaag
    13081 ggcgattcgg cgctgaccgc cacccactac tgccccggtt gcgagcacgg cgtgctgcat
    13141 aagttgatcg gcgaggcgct ggccgacctg aacctgcaag accgaaccgt gatgatcagc
    13201 cccgtcggct gcgcggtgtt cggctattat tattttgata cgggcaacat ccaagtggcc
    13261 cacggccgtg cggcggcggt ggccaccggg ctctcccgca ccatgaacca agcggtggtc
    13321 ggcgcgtatc agggcgatgg cgatttggca tccatcggcc tcaacgaaac cctgcaagcc
    13381 gccaaccgcg gcgaaaaact ggccgtgttt ttcgtcaata attcggtgta tggcatgacc
    13441 ggcggccaga tggcgccgac cacgctggtg ggcgagaaaa cggaaacctc cccggagggg
    13501 cgcgacccgc tccagatggg ctacccgctg cacatggcgg agctgatcaa ccagttgcag
    13561 gcgccggtgt acattgcgcg ggtgtcgttc tccaacatgg ccaagctgcg gcagggccgg
    13621 caggccatcc gcaaggcgct ggaaatccag cgcgacggca agggctacgc attcgtggaa
    13681 gtgctcatgg cctgccccac caaccagaaa atgaacgtgg aagccgccac gcggttcgtc
    13741 gccgagcaaa tggaggcgga atatccgctg gggattttcc gcgaccgctc cgccgaggcc
    13801 cagccgattg tccgcagcga gagcgattac cgcaaggaga ccctcgaccg cctgctgggg
    13861 ctggatacgg atcaggcgcc ggatgcccag ttggaccccg ccgcgccaaa atggaaaatc
    13921 aagatggcgg gttttggcgg ccaaggcgtg ttgagcatgg gcgtgatttt ggctcgcgcg
    13981 gcgtgccgcg ccggcaagtt tgtgtcgtgg tatccctcct acggccccga gcagcgcggc
    14041 ggcaccgcca attgcggcgt ggtgttcgcc ggccagcctg tgggctcgcc gatggtaacg
    14101 gaagcggacg tgctggtggc gatgaaccgg ccatcgctgg aaaaatttgc ggcggaagta
    14161 aaacccggcg gctggctgtt gtataacgcg gctattggcg acttcacgcc gccggcgggc
    14221 gtgcgcgcgc tggctgtgcc ggccaacgag ctcgcccaag agggcggcaa cccgcgtggc
    14281 gccaatacgg ccatgctggg cgtgctggcg gaactgggcg gcttcggcct cgcggatgaa
    14341 cactaccgcg ccgccatcgc cgccaacttc gccggacgcg aaaaaatcat tcagcaaaat
    14401 caagccgtct ttgcccacgc ccgcgcctgg gccgcaaaac aacaaattcc gcactaaccc
    14461 accggcatga cccggatctt tttgcgggcc gggaacggct ggaagttctg cgaccgttcc
    14521 agccccacaa accaaccggc tgcttttgtc gggcgggaat ggttgaggaa cgacaccttc
    14581 ccgccgcgaa tggctgaaaa gtctgcaatc gcatcagcca aaatttgaat gattgcctaa
    14641 ccacccggtt cgtctatgat ggtgccaatg cggtgctgga gttgtcagcc gaaggcagat
    14701 ccgcctatgg aggaaacgtg agctaccaag tgatttgggc gtgggtgaat gcgccggggc
    14761 tggatcagcc ggtcgagcgg attgcgttca tcaacgggga agcccgccag cggcaggttt
    14821 tccatgccga tgggctcggt tctatcgccg cgctgacgga cgagagcggc gcaaccatcc
    14881 aaacctacgc ctatgaagcc ttcggcagca tccgcaccca aaccggcacc gacctcaacc
    14941 gcgttacctt caccgcccgc gaagctatgg gcgacaccct cggcttcttc tactaccgcc
    15001 accgcatcta cgatccccac accggccgct tcacctccga agacccgctg gggtttgccg
    15061 ctgacccaaa tcgctatgtt tacgttataa atagtcctgt taacatgaca gatgcattag
    15121 gcatggaagg agaaggattc gatagtgatc cgataattaa agactttttt gacaaactat
    15181 ctcaagatgc agaatggttt agcgaaaatg agcatgaaga aggtggacat gttaatcaat
    15241 gcatcacaaa tggggaagta acgcctgttc aatggccgcc aggaactact taccgcattc
    15301 ccgttccgga ccaataccat atagataaaa gcaatccaaa tataatagtg ctacttacag
    15361 ttcatactca tcccccgtct gagcctaaac caagtgatca ggacaaaaca aatgcaaatg
    15421 caaggggggt accccttgtg attctaaatt tcctcgaaaa taacacctat tatccgcctc
    15481 catgtgataa ttgagggaaa gagttaatgc gcgcaaaata tgatttatta attgtatgct
    15541 ttggattgtg ttgtctatcg caattattag agtcacatgc cggatatata gaagttatat
    15601 gggaaaatta tccggcacct tatatatcca gcttgtatgt aaactcatca ggagaatatg
    15661 catatcgcac aaagtatgac gatacaaaag tcgagagaaa taacatagcc agaatattaa
    15721 atatggccgg atcaaattca gcttactcga taaagatcaa ggctaatatt gatacatatg
    15781 tcgccgacaa tatcgaacac gtttcatatg aaagatcatt atctcaactt gttgaatatt
    15841 accggaataa tagtaatata attgtcgcta aaccgggcga tatcaatatt gaagatggct
    15901 ggaagattca aataatattt tatgatgatg tggaaatata ttcagtgata tttcgggaca
    15961 atccggacat tcattgcgcc agaatgttgt ctattattca ggagatcggc agcgtattcg
    16021 ccgagtacct ttcaacgccc ttgagttggg cagtgcatgg cgttgagata tttccggaaa
    16081 gggaatgaaa ggggcgatcc tatataagga aaggggtcgg tcctataaag taataaaata
    16141 gattgacgtt gaaagaatgg aaatagtgca aaaggaacag gaaaggggtc ggtcctataa
    16201 agtaataaaa tagattgacg ttgaaagaat ggaaatagtg caaaaggaac atgccaaggg
    16261 aacccagagt ggaatatgct gggcggtctt ccacgcgatg aggcggggca gccggttgtc
    16321 ttcgacggcg caggcaatcg catcagccaa agtctgaacg attgccttac cacccggttc
    16381 gtttatgatg gtcccaacgt ggtgatggaa atgtcagccg gaggcggatc cgcctatgga
    16441 ggaaacgtga gccaccaggt gatttgggcg tgggtgaatg cgccggggct ggatcaaccg
    16501 gtcgagcgga ttg
//
LOCUS       DAOPNP010000050        16458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4199_length_16458_cov_10.068768, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000050 DAOPNP010000000
VERSION     DAOPNP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_16458_cov_10.068768"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1815)
                     /gene="ilvD"
                     /locus_tag="PKI64_06035"
     CDS             complement(115..1815)
                     /gene="ilvD"
                     /locus_tag="PKI64_06035"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE00606.1"
                     /translation="MSTKKKHSGKSLHRQWSKDVTDGMERAAGRAMLHAVGFTAADFQ
                     KSQVGIASTWSQVTPCNIHIDRLARRAAEGVAAAGGKPVIFNTITISDGISMGTQGMR
                     YSLVSREIIADSIESVVGCEAFDGLVAIGGCDKNMPGCLIAMARLNRPAVFVYGGTIK
                     PGCHHGKPVDIVSVFEAVGQKARGEISGKELAAIEGCAIPGAGSCGGMYTANTMASAI
                     EALGMSLPNSGAQNAVSAAKTRDCREAGRAVMEMIRRGIRPSDILTREAFLNAIALVM
                     AMGGSTNAVLHLLAIAHSANVKLELDDFTRIAARTPVLADLKPSGKHVMSALVSIGGT
                     APLMKLLIEEGLMNGDLLTVTGQTMRQNLKNIRPYPKGQTIIRPPSDPIKPTGHLVIM
                     RGNLAPEGAVAKISGHEGLQFSGRAKVFESEETALAAILQGQVKKGHVVVIRNEGPKG
                     GPGMREMLSPTSAIMGRGLGRDVALITDGRFSGGSHGFVVGHISPEAAVGGPIALVRN
                     GDPIVIDAEKRTITLAIPAAELKRRRAAWRQPPPKETRGALAKYARQVGSASFGAVTD
                     "
     gene            1993..3675
                     /locus_tag="PKI64_06040"
     CDS             1993..3675
                     /locus_tag="PKI64_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOE00607.1"
                     /translation="MAWLLAAGAAVAGGGPLNTLVVVNAASRDSRALGAYYLAKHGIP
                     QDHLCSITVSPRASSVTLADFERSIRAPILAHLANNGLQGQIHYIVLCMDIPSRVENS
                     NGITAALFYGYKQQPSDGKTPCHMATNSLNQYYGAEMAYTATAGWNRTNAPIVFLLTA
                     RDLKTAKQVVDRGVAAQGTFADGLFCLAGSADTARNIRHRAFPAVARQFALHGQSARL
                     LAEPTTSPVPEQPVLSYLTGLAYFPSNFTKLVFAPGAIADNLTSCAGQLPLPCENQSS
                     VWDWMQLGATASYGTVSEPCAYEAKFPDPMLAYWYFRGFTAGEALAMSVRNPYQGIWV
                     GDPLAAPFAAPPVVQISAPTANAVLTGPTNLHLAVAAHERGAPPVYLDLYVDGRHHAP
                     ITRPFAAVGNDLTAEIGTNRFVYTVAPGEDLFAAVAGLAWAINARGGGNITASVRADR
                     LDISVRQPLGSDGEPLPCAVGSTTGFSKGLYIGGATGRDRLLVSDGVGRASATFHLGT
                     ARAFEVDYPLNLATLPPGMHTLTVVARDGTAVQCQSQVTLPFRTIPAPSSAQ"
     gene            complement(4007..4699)
                     /locus_tag="PKI64_06045"
     CDS             complement(4007..4699)
                     /locus_tag="PKI64_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00608.1"
                     /translation="MATDPPTPSRRDSFATDAARAERRQREANLRKDAARQRLRLVFM
                     LFAAAALVALVMDARNMSRRLQTRTAQQPTPRKGDVADIEAGMHDARYVDPSGLFSLV
                     PPRHWVKVKQPPGSFFNVVFQGPHGMDMSILVSVTNGITFNALVDNLRQRERALAAET
                     HLDFAYVGPHRAVKRTVQLFRSKLLLLDFLTGDLAHHVQFSTPPELFDEYEPVFLRLM
                     QTYEPGRILRAP"
     gene            complement(4689..5636)
                     /locus_tag="PKI64_06050"
     CDS             complement(4689..5636)
                     /locus_tag="PKI64_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00609.1"
                     /translation="MQEHDFLRKCVGALLGVAVAMAAAVFISWWLAALPADVAAPPPK
                     PARSAAAGAPVAAPVPPAPAHAPAPAALPPPARPVAELPAADADAEALAKKAFRLEME
                     NARLRHRLDDMLNWLLENVRGTFPLPEEQMANLRIVPVDTNLQVSADLAQILRLNDAE
                     VNTLDEAFLGTRTLLYELETEKLTVQQPAAGQAVLNMPPYPEEGQLVREELYGTLQRT
                     LGPARFSRFRQVAEDGLDEKFEYFGEVERTIEFEEVVDAESGSPQLFVRDERILPSRD
                     DPQRQDITTSERLVSELPAEYYAYWNWLPDYVTRYARGH"
     gene            complement(5665..6021)
                     /locus_tag="PKI64_06055"
     CDS             complement(5665..6021)
                     /locus_tag="PKI64_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE00610.1"
                     /translation="MAEAVLTTQIENRDGVQVIHVSGPLDSMTHDRFKDLLDPLVNQS
                     KMRIVLNCENLTYVNSKGLALLGRYQRISLQNLSFMGIAALNKRITKTIELLGMSKLV
                     KLYPSVADAMQAATEQ"
     gene            complement(6161..6517)
                     /locus_tag="PKI64_06060"
     CDS             complement(6161..6517)
                     /locus_tag="PKI64_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE00611.1"
                     /translation="MAEAMLKFEQEERDGVLIVHVNGPLDSMTHDQFREYLGPIMNRP
                     KVRVVLDCQNLSYINSRGLTQLMHYQRISKIAFSFLGVAALRPNIVKNLELLGLAKTF
                     RWYPTVEEALTAATAY"
     gene            complement(6617..8872)
                     /locus_tag="PKI64_06065"
     CDS             complement(6617..8872)
                     /locus_tag="PKI64_06065"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOE00612.1"
                     /translation="MKQAGQWWWMLGLALLAAGCGGPRPEGPPVTVASLTEPLTDPLW
                     LPRLDQPDTILHTSYDRTGGNDDYGTFLRDAAEPGWKVIADLKGPGYVSRFWFTGAKD
                     GFPHHIRFYFDGETTPRLAGDIKELFGGEFAPFLAPLAEYKGYCWYSFVPLPYAKSLR
                     IECEAGPPGAKVYYQVSESRLPRGTAVETFAWPLPERDLAALATAQRLWASGELPAQG
                     EPQEATLTPGRPGSLRVAGPAVITRLEFTPDWAKLAEDARDSVLRDWQVSIRYDGLTN
                     ESVKVPLGDFCGAPWRRLRAQSLYFGMRGDSLFCALPMPFAQSAEITLEAGRIAGVPV
                     NVRAWVAPIPAAELAHLGYFHAGWRRTTPQDVGRPHPILHATGRGKFVGCLLAVCTLD
                     GSYWALEGDESIRKDRETTPGWLGTGLEDYFNGGWYYQNPMAGPTHGLFVKEPFRTVQ
                     YRVHSMDPSQFLSSLDMVFERGPDHKSRAYFESVSWYYLVRPQTADTMRLRAEDRRMP
                     PDPRLDGIALMSVLWNYERFGDWAGERDELAQRLRRHGDRLTPTARRMLELRLALLTE
                     KLGGADPLPAFANDADPAVRDAVGLLQQQRAGQGALAVFYANMPARLYLNGQEVLQAG
                     NPEKPVAALLSLTPGRHQLAIQTPRQSYPDWVQLALRYRDQFFGTDPSWKFAFNPTGD
                     WASPAYDDSAWRPCGGTGVKGPPEEPYVWVEPDPWLDMQSRAIGIRPQGDWPAGGGFV
                     VYRQTIEVREK"
     gene            complement(8997..9371)
                     /gene="rplT"
                     /locus_tag="PKI64_06070"
     CDS             complement(8997..9371)
                     /gene="rplT"
                     /locus_tag="PKI64_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOE00613.1"
                     /translation="MPRVTNSPASRKRRKGRLALAKGFYGARSKQFRLATEAVDRAMQ
                     LNYTHRKEKKRDFRRLWTTRLSAACRPHGISYSRFIEGLAKAGVLLNRKMLSEIAIHD
                     ADGFASLVTLAKSKATLPAKKA"
     gene            complement(9402..9593)
                     /gene="rpmI"
                     /locus_tag="PKI64_06075"
     CDS             complement(9402..9593)
                     /gene="rpmI"
                     /locus_tag="PKI64_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOE00614.1"
                     /translation="MKKKTRKSVAKRFKRTAKGKILHKKPGRRHLAASKTRKQKRRLR
                     QNVQAGGKIAKSLGIELGA"
     gene            complement(9765..10346)
                     /gene="infC"
                     /locus_tag="PKI64_06080"
     CDS             complement(9765..10346)
                     /gene="infC"
                     /locus_tag="PKI64_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014610565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOE00615.1"
                     /translation="MAIPPPTNPRDPRGGFPTRINHRIRVPEVFLIGPGGEQLGVVAT
                     ADAQARAQAAGLDLVEISPTARPPVCRIMDYGKFQYEKSKKEKQNRRNAAAVKVKEVQ
                     LHPSVGDNDYQVKLRRMREFLEDGHRVKVALFFRGRENAHKELGFVLMNRVLADLREV
                     GVVEQAPKLMGRNILMVLIPNPKARQQGAAASR"
     gene            complement(10348..12129)
                     /gene="thrS"
                     /locus_tag="PKI64_06085"
     CDS             complement(10348..12129)
                     /gene="thrS"
                     /locus_tag="PKI64_06085"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOE00616.1"
                     /translation="MKVEQVALETMRHSTAHVMAAAVRRLWPDALFDIGPDTEDGFYY
                     DFDLAHRLTAEDFPAIEAEMAKIVAENQPFVREEKSRAEVWQMMTERGQKYKLERLAD
                     IPDGETISLYHSGDFTDLCRGPHVARTGDIKAFKLLSVAGAYFRGDEKNQMMQRLYGT
                     AFLTQADLDAHLKMLAEAKLRDHRTLGRDLDLFSIQEAVGPGLIHWHPKGARIRTIIE
                     EFWRREHYRAGYEMLYTPHVGRAGLWETSGHLGFYSENMYAPMEIDEQRYYIKPMNCP
                     FHIQIYQAHKRSYRELPLRWAELGTVYRYERAGVLHGLMRVRGFTQDDAHIYCTEEQV
                     ESEVRECVRFAIAMWRAFGFENITAYLATRPEKAVGDPARWELAQRSLEAALQAEGIA
                     YELDEGGGAFYGPKIDLKTRDAIGRSWQMSTVQFDFNLPERFDMAYIGPDGQEHRPFM
                     VHRALLGSLERFFGILIEHYAGAFPLWLAPVQVGLIPVTDAQADAARAVAAQLKARDI
                     RAVVDERSEKLGAKIRDAQLQKIPYMLVLGKREVEQGTVSVRSRVAGDLGVMPLDAFI
                     AKVDAERQVALPTTNANGAAHGSQPQG"
     gene            complement(12206..13231)
                     /gene="mvaD"
                     /locus_tag="PKI64_06090"
     CDS             complement(12206..13231)
                     /gene="mvaD"
                     /locus_tag="PKI64_06090"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOE00617.1"
                     /translation="MTPRDVVARILAGRGSTPRAVANAYAPANIALVKYWGKRDEALN
                     LPVTNSLSISLGPLGSHVELARGAGAADAIWLNGEPIPAESSFARRASAFLDWFRPAP
                     DFTFDLRARNTVPTAAGFASSASGFAALVKALNALFGWDLPPREQSILARLGSGSAAR
                     SLADGFVEWHAGAAPDGMDSFAAPLEATWPELRVGALVLCAAEKPVGSREGMRRSVAT
                     CEFYREWPARVARDLAELKTAIAARDIAALGTVAEGNALAMHALMAATRPPLVYALPE
                     TMTALHAIWAARRDGLPLWFTMDAGPNVKLIFTRAAESQVRALFPAVQVVAPFASPAE
                     NPPPAHS"
     gene            complement(13228..14166)
                     /locus_tag="PKI64_06095"
     CDS             complement(13228..14166)
                     /locus_tag="PKI64_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOE00618.1"
                     /translation="MTLRAVKASAPGSLMLMGEHAVLRGQPALVCAVSKRLTVKLTPR
                     TDETIVLHSALGEHETTLGELAPHDSFRFALGAIRALREGLAQGFELDIRSEMSHVMG
                     LGTSAAVTLAVLAALRHAQGLSIAPEELVVPAIQVVRRAQGGVGSGADVAASAYGGCL
                     RFHAESTEVVQLPTAPQLTVLYSGSKMPTPEVIAVVEAQRKAQPELHARLDDLIGDAV
                     NRAFEAAARGDWPAMGALMNINQGLMDALGVNNAKLAALVYELRADPKIHGSKISGSG
                     LGDCVVGLGKAMRTEWGVPALAVQVTPAGVLAEALP"
     gene            complement(14177..15190)
                     /gene="mvk"
                     /locus_tag="PKI64_06100"
     CDS             complement(14177..15190)
                     /gene="mvk"
                     /locus_tag="PKI64_06100"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOE00619.1"
                     /translation="MKAVAPGKLILTGEHAVVYGRPALAMAVNRNAQVFVTMDGAAET
                     VSFDLPNVTAPKESFTLRALRDFHARVRRNYEEFLAGKLSIREVLLKPVDLFQYAFVT
                     VLDGLHLKMGGGVNVRLNSNIPIGCGMGSSAAAILSVLRGLGHYYRVDFRPEWYARYS
                     LEVENMQHGKASGLDTYVSLHGGCVRFQQGAAQELPLPRMPLYIVQTGTPASTTGEAV
                     SDVARRLGAQVRVWDDFETVANQMQRALEQDNLKDFQCAMRDNHRLLNTIGVVPDRVA
                     EFVAAVEESGGAAKICGAGAVTGEQAGMVLVAAREAPADLCAKFGYEMLTVRPDPLGV
                     RVV"
     gene            complement(15416..16222)
                     /locus_tag="PKI64_06105"
     CDS             complement(15416..16222)
                     /locus_tag="PKI64_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00620.1"
                     /translation="MRKRIRLFFGLLAFCVTQAPGDTIGFFYALDADLQALKTQAREM
                     GQATRVGSRSVQRLQLDAHTIYAVKMGSGAVETAVSVQALLSRFRCDWAFSIGPAGAL
                     SDEMATHQWHRVGSVTAWQTDSQAKTWRTDWTRLPVAVFPSPLQTTSTIAVASGEQFI
                     STTRERDRLQAMTQADAVDMNSFGLALVCADHGVPLFAWKIISDHADETASETFRSFI
                     ATYTGEGGKALIEIIRALPANPNDPSSYPVIERLLHEVPTENQLPNDSRS"
BASE COUNT         2994 a         5259 c         5214 g         2991 t
ORIGIN      
        1 ctgtggaaaa aacgacggca aaagttccac actgtggaaa agagttttcc acagtgtgga
       61 aaaggtgaag cttgagtgct tccgaataga ctggcgagct gttccgctgg gtggttagtc
      121 ggtgacggcg ccgaaggagg cgctgccaac ttggcgggcg tatttggcga gggcgccgcg
      181 ggtttccttg ggcggcggct gacgccatgc tgcacgacga cgcttgagtt ctgcggcagg
      241 gatggcgagg gtgatcgtcc gcttttcggc gtcaatgacg atgggatcgc cattgcgcac
      301 gagggcgatg ggtccgccca cggcggcttc gggggaaata tggcccacca caaagccgtg
      361 gctgccgccg gagaagcggc catcggtgat caaggccaca tcgcggccga ggccgcggcc
      421 catgatggcg gaggtgggcg agagcatctc gcgcattccg gggccgcctt tggggccttc
      481 gttgcggatg accaccacgt ggcccttctt cacttggccc tgcagaatcg cggccaacgc
      541 ggtttcttcg gattcaaaca ccttggcgcg accggagaat tgcagtcctt cgtgcccgct
      601 gattttggcc acggcgcctt cgggggcgag gttgccgcgc atgatcacca gatgccccgt
      661 gggcttgatg ggatcggaag gcggccggat aatggtctgc cccttgggat aaggccggat
      721 gttcttgagg ttctgccgca tggtttggcc ggtgacggtg agcaggtcgc cgttcatcag
      781 cccttcttcg ataagcaatt tcatgagcgg ggcagtgccg ccaatgctga cgagggcgga
      841 catgacgtgt ttgccgctgg gtttgagatc ggccagcacg ggggtgcgcg cggcgatgcg
      901 ggtgaagtcg tcgagttcca atttgacgtt ggccgagtgg gcaatggcca gcagatggag
      961 cacggcattc gtggagccgc ccatggccat gacgagcgcg atggcgttga ggaaggcctc
     1021 gcgggtcaga atatcgctgg ggcggatacc gcggcgaatc atctccatga cggcgcggcc
     1081 ggcttcgcgg cagtcgcgcg ttttggcggc ggacaccgcg ttttgcgcgc cgctgttggg
     1141 caggctcatg ccgagggctt cgatggcgga ggccatggtg ttggcggtgt acatgccgcc
     1201 gcaggagccc gcgcccggaa tggcacagcc ttcgatggcc gccaattcct tgccggaaat
     1261 ttcaccgcgc gctttttggc ccacggcttc aaagacgctg acgatatcca cgggcttgcc
     1321 gtggtggcag ccgggcttga tggtaccgcc ataaacgaat accgcggggc ggttgaggcg
     1381 ggccatggcg atgaggcagc caggcatgtt tttatcgcac ccgccgatgg cgaccaggcc
     1441 atcgaacgcc tcgcagccga cgacggattc gatggagtcg gcgatgattt cgcgcgagac
     1501 gagggaatag cgcatgcctt gcgtgcccat ggaaatgccg tcggaaatgg tgatggtgtt
     1561 gaagatcacc ggtttgccac ccgcggcagc gacgccttcg gcggcgcggc gcgccagccg
     1621 gtcaatgtga atgttgcacg gggtgacctg gctccacgtg gaggcgatgc ccacctgtga
     1681 tttctggaaa tcggcggcgg tgaagcccac ggcgtggagc atggcgcggc cggccgcgcg
     1741 ctccatgccg tccgtcacgt cttttgacca ttggcgatgc agagatttgc ccgaatgttt
     1801 cttttttgtg ctcatgcgtg ttttcctgtg ctggttccgg cgttagcctg aactatcatc
     1861 ctaccccgcg ccatggttgg atgcaaatcc ggcggcggac aaaccgttgc ggcggggcgc
     1921 ttcccggctt gaactttggc ggggcatccc gcacatttcc caacaatgaa aggatggttt
     1981 cgggtttggg tcgtggcgtg gctgctggct gccggcgcgg cggtggcggg cggcgggccg
     2041 ctgaatacgc tggtggtggt gaatgccgcc agccgcgatt cccgcgcgct gggggcatat
     2101 tatctcgcca agcacggcat tccgcaggac cacttgtgca gcatcacggt ctcgccgcgc
     2161 gcgtcttcgg tgacgctggc cgactttgag cgcagcatcc gcgcgccgat tctggcgcac
     2221 ctagccaaca acggtctgca gggccagatt cattatatcg tcctgtgcat ggatattcca
     2281 tcgcgggtgg agaacagcaa tggcatcacg gcggcgctgt tttatgggta taagcagcag
     2341 cccagcgacg gcaaaacgcc gtgccacatg gcgaccaatt cgctgaacca gtattacggc
     2401 gcggagatgg cctacaccgc cactgccggc tggaaccgga ccaacgcgcc gattgtgttt
     2461 ctgctgacgg cgcgcgatct gaaaaccgcc aagcaggtgg tggaccgcgg cgtggcggcg
     2521 cagggcacct ttgcggacgg gctgttttgt ctggcggggt cggcggacac cgcgcgcaac
     2581 atccggcacc gggcatttcc ggcggtggct cgccagtttg cgctgcatgg ccagagcgcg
     2641 cggctgctgg ccgagcccac caccagcccc gtgccggagc agccggtgtt gagctatttg
     2701 accggcctcg cgtattttcc atccaacttc acgaagctgg tatttgcgcc gggagccatc
     2761 gcggacaatt tgacttcgtg cgcgggccag ttgccgttgc cgtgtgaaaa ccaaagtagc
     2821 gtgtgggact ggatgcaact gggcgccacc gcttcctacg gcacggtcag cgagccctgc
     2881 gcctatgagg ccaagtttcc cgatccgatg ttggcttact ggtatttccg cggtttcaca
     2941 gcgggcgagg cactggcgat gtcggtccgc aatccgtacc aaggcatttg ggtgggcgat
     3001 ccgctggccg cgccgtttgc cgcgccgccg gtggtgcaga taagcgcacc gacagccaac
     3061 gcggtgctga ccgggcccac caacctgcac ttggcggtgg ccgcgcacga acgcggcgcg
     3121 ccgccggttt atctggattt gtatgtggac ggccggcacc acgcgccgat cacgcgcccc
     3181 ttcgcggcgg taggcaatga cctcacggcg gaaattggca ccaatcgctt tgtctatacc
     3241 gtggcgccgg gcgaggatct ttttgcggcg gtggccggcc tggcgtgggc catcaatgcg
     3301 cgcggcggcg gcaacatcac cgccagcgta cgggccgacc ggctcgacat ttctgtgcgc
     3361 cagccgctgg gcagcgatgg cgaacccctg ccctgcgcgg tcggcagcac gaccggtttt
     3421 tccaaggggc tgtacatcgg cggggccacc gggcgcgacc ggctgctggt cagcgacggc
     3481 gtggggcgcg ccagcgccac gttccatttg ggcacggcgc gcgcgtttga ggtggattat
     3541 ccgctgaatc tggccacgct gccgccgggg atgcacacgc tgaccgtggt ggcgcgcgac
     3601 ggcaccgccg tgcaatgcca atcgcaagtg acgctcccct tccgcacgat ccccgcgccc
     3661 agcagcgcgc aataaaggcc acgccgcgtc gctcagggcg cgcgcaaaat ccgacccggc
     3721 tcgtaggagg gctggaacgg tcgcagacct tccagccgtt cccggcggga aggtatcgtt
     3781 cctcaaccat tcccgcccta caaaagcttc cggttggatt gtggggctgg aacggtcgca
     3841 gaccttccag ctattcccgg cgggaaggtt tcattcttca accattcccg ccctacaaaa
     3901 aagcccgatt cagcaatatc gttgggtatc ccaatgcgga actcatgctc cccttccgca
     3961 caattcccgc gcccagcagc gcgccataaa ggccacgccg cgtcgctcag ggcgcgcgca
     4021 aaatccgacc cggctcgtag gtttgcatca gacgcaaaaa gaccggttca tattcatcga
     4081 acaactctgg cggcgtgctg aactgcacat ggtgcgcgag gtcgccggtg agaaaatcca
     4141 gcagcagcaa cttgctgcgg aagagctgca cggtgcgttt cacggcgcgg tgcgggccca
     4201 cgtaggcaaa gtccagatgc gtttcggcgg ccagcgcgcg ttcgcgctgg cgcaggttgt
     4261 ccaccagcgc gttgaaggta atgccgttgg tgacgctgac caggatgctc atgtccatcc
     4321 cgtgcgggcc ttgaaaaacc acgttgaaaa aactgccggg cggctgcttc actttgaccc
     4381 agtggcgcgg cggcacaagg ctgaacaggc cggaggggtc cacgtagcgg gcatcgtgca
     4441 ttccggcttc gatatcggcc acgtcgccct tgcggggggt gggctgctgg gcggtacggg
     4501 tttgcaggcg gcgcgacata ttgcgcgcat ccatcaccag cgcaaccagc gcggcggcgg
     4561 caaacagcat gaacaccagg cgcagacgct ggcgggcggc atccttgcgc aggttggcct
     4621 cgcgctggcg ccgttccgcg cgggcggcat cggtggcaaa ggaatcccgg cgggacgggg
     4681 tgggaggatc agtggccacg tgcgtacctc gtcacatagt cgggcagcca gttccaatag
     4741 gcgtaatatt ccgcgggcag ttccgagacg aggcgctcgg aggtggtgat gtcctgccgc
     4801 tggggatcat cgcggctggg gaggatgcgt tcatcgcgca cgaacaactg ggggctgccg
     4861 gactcggcat ccaccacttc ctcgaactcg atggtccgct caacttcgcc gaaatattca
     4921 aacttttcgt cgagcccgtc ctccgccacc tggcgaaagc ggctgaagcg cgctgggccg
     4981 agggttcttt gcaacgtgcc gtagagttct tcgcgcacca gttggccctc ttccggatag
     5041 ggcggcatat tcagcacggc ctggccggcg gcgggttgct gcacggtgag tttttcggtt
     5101 tccaattcgt agagcagcgt gcgggtaccg aggaatgcct catccaaggt gttcacttcg
     5161 gcatcgttca gccgcaggat ttgcgcgaga tcggcgctga cctgcaaatt ggtatccacc
     5221 ggcacgatgc gcaggttggc catttgctcc tcgggcagcg gaaaggtgcc gcgcacattt
     5281 tcaagcagcc agttgagcat gtcatcgagc cgatgccgca ggcgtgcgtt ttccatttcg
     5341 aggcggaaag cttttttggc gagcgcttcg gcatcggcat cggctgcggg cagctcggca
     5401 accggccttg ccggcggggg aagggcagcg ggcgcgggtg cgtgagccgg cgccgggggg
     5461 acgggcgcgg ccaccggcgc accggcagcg gcagaacgcg cgggtttggg cggtggcgcg
     5521 gcgacatccg ccggcaacgc ggccagccac cacgagatga acacagccgc cgccatggcc
     5581 acggccacgc ccaacagcgc gccgacgcac ttgcgaagaa aatcgtgttc ctgcattacc
     5641 gccccgcgaa tgggccggct ccggctactg ctccgtggcg gcttgcatgg catccgccac
     5701 cgacggatac aacttcacca gcttgctcat gcccagcaat tcaatggtct tggtaatgcg
     5761 cttgttcagc gcggcaatac ccatgaagga caagttttgc agcgatatgc gctgatagcg
     5821 gcccagcagg gccagccctt tgctgttgac ataggtcagg ttttcgcagt tcagcacaat
     5881 gcgcattttg gattggttga ccagcggatc gagcaaatcc ttgaaacggt cgtgcgtcat
     5941 ggaatccaac ggaccggaga cgtggatgac ctgcaccccg tcgcgattct caatctgcgt
     6001 tgtcaagaca gcttccgcca tgcccacgtc tccctaacat ccggcgcggg tttcagcccc
     6061 gcgctttcaa tgtgataaac ctacccgctc ccccgttggt ttgtcacgcc caaaaacagg
     6121 ggccggtaaa aatacaccgg tcgcgacgcg gcgggggggg tcagtaggca gtggcggcag
     6181 tgagtgcttc ctcgaccgtg ggataccagc ggaaggtctt ggccaggccg agcaactcaa
     6241 ggtttttgac aatgttgggc cgcagcgcag ccacgcccaa aaaggaaaac gcgattttgc
     6301 tgatccgctg atagtgcatc aactgggtca ggccccggct gttgatatag gacaggttct
     6361 ggcagtcgag caccacgcgc actttgggac ggttcatgat ggggcccaga tactcccgga
     6421 attggtcgtg ggtcatggaa tccagcgggc cgttcacgtg cacaatcagt acgccgtcgc
     6481 gctcttcctg ctcaaacttc aacattgctt ctgccatggc gacctccact ctcaaacgta
     6541 ccacaccttg ccccggctgg gaaaaaccgg tggcggcgtt aacagaatcc taacaatttg
     6601 ccggtgcagc gcaaaatcat ttttcgcgga cctcaatggt ttggcggtac acaacaaagc
     6661 cgccgccggc gggccagtcg ccttgcgggc gaatgccgat ggcgcgcgac tgcatatcca
     6721 gccatggatc cggctccacc caaacatagg gctcctccgg tgggcctttg acaccggtgc
     6781 caccgcaggg ccgccacgcc gaatcatcat aggcggggct ggcccaatcg ccggtgggat
     6841 taaacgcaaa cttccacgag gggtccgtgc cgaaaaattg atcgcgataa cgcagggcca
     6901 actgcaccca atccggatag gactgtcgcg gggtctgaat ggccagttga tggcggccgg
     6961 gcgtcagcga cagcaacgcc gcaactggtt tttccgggtt gccggcttgc aggacttcct
     7021 gcccattcag atacaaacgc gcgggcatgt tggcataaaa cacggccagc gcgccctgcc
     7081 ccgcccgctg ctgttgcagc aggcccaccg cgtcgcgcac ggcggggtcg gcatcgttgg
     7141 cgaacgcggg cagcgggtcc gcgccaccaa gtttttccgt caacaacgcc aggcgcagct
     7201 ccagcatccg gcgcgcggtg ggagtcaggc ggtcgccatg acggcgcagc cgttgcgcca
     7261 gttcatcgcg ctcccccgcc caatcgccga agcgctcata attccacagt accgacatca
     7321 gcgcgatgcc atccaggcgc ggatccggcg gcatacgccg gtcttccgcc cgcaggcgca
     7381 tggtgtcggc cgtctgcggg cggaccagat aataccaact gacgctttcg aagtaggccc
     7441 ggcttttgtg atcgggcccg cgttcgaaca ccatgtccaa tgatgacaaa aactgcgacg
     7501 ggtccatgct gtgcactcgg tattgcaccg tgcggaacgg ctccttcacg aacaggccgt
     7561 gcgtgggccc ggccatggga ttctggtaat accagccgcc gttgaaataa tcctccagtc
     7621 cggtgcccag ccagcccggc gtggtttcgc gatccttgcg gatggattca tcgccctcca
     7681 gcgcccaata actgccatcc agcgtacaca ccgccagcag gcagccgaca aacttgcccc
     7741 ggccggtcgc gtgcaaaatg ggatgcggcc gcccgacatc ctgcggcgta gtgcgccgcc
     7801 agccggcatg gaaatagccc aagtgcgcga gttccgccgc ggggatgggc gcgacccacg
     7861 cgcgcacgtt gactgggacg ccggcgatcc gcccggcctc cagcgtgatc tctgccgact
     7921 gcgcaaatgg catgggcagc gcgcaaaaaa gcgagtcccc ccgcatgccg aaatagagcg
     7981 attgcgcgcg cagccgccgc cacggcgcgc cgcaaaaatc gcccaacggg accttgacgc
     8041 tctcgttggt gaggccgtcg tagcggatgc tcacctgcca atcgcgcagc acggaatcgc
     8101 gcgcgtcttc ggccagtttt gcccagtccg gcgtgaactc caatctcgtg atgactgccg
     8161 gccccgcgac gcgcaaactg ccgggccgtc cgggtgtcag cgtggcttcc tgcggttcgc
     8221 cctgtgcggg caactcgccg gaagcccaca gacgttgcgc ggtcgccagt gcggccagat
     8281 cccgctccgg cagcggccac gcgaaggttt ccaccgcagt gccgcgcggc aggcgcgatt
     8341 ccgaaacctg gtaatacacc ttggcgcccg gcgggccggc ttcgcactcg atgcgcagcg
     8401 acttggcgta gggcagcggc acgaacgaat accagcagta gcctttgtat tccgccaacg
     8461 gggcaagaaa cggagcaaac tcgccgccga ataattcttt gatgtcgccc gccagccgcg
     8521 gcgtggtttc gccgtcaaaa taaaagcgga tgtggtgcgg gaagccatcc ttggcgccag
     8581 tgaaccagaa gcgcgagaca tagccggggc ccttgagatc ggcaataacc ttccagcccg
     8641 gttcggcagc atcgcgcaaa aatgtgccgt agtcatcgtt gccgccggtg cggtcgtagg
     8701 acgtgtgcag aatcgtgtcg ggctgatcaa ggcgcggcag ccacaggggg tccgtgaggg
     8761 gttccgtcag gctggccacg gtcaccggcg gcccctccgg gcgggggccg ccgcagccgg
     8821 ccgccagcaa cgccaaaccc aacatccacc accactgtcc agcttgtttc atgcgcacct
     8881 cgggcagcga ctatgcccca acgcagccca ccagcgcaac cgccaatgcg cagcgcacgg
     8941 gcataaaaaa agcgcggggg caaaacctcc gcgcggagaa cggccaataa ccggactcaa
     9001 gccttcttgg cgggcaacgt ggccttgctc ttggcgaggg tcaccagcga ggcaaaaccg
     9061 tcggcatcgt gaatggcgat ttccgagagc atcttgcggt tcagcaacac gccggccttg
     9121 gccaggcctt cgatgaagcg ggaataggaa atcccgtgcg ggcggcaggc ggcgctgagg
     9181 cgcgtggtcc acagccggcg gaaatcgcgt ttcttttcct tgcggtgcgt atagttgagc
     9241 tgcatcgcgc ggtccacggc ttccgtggcg aggcgaaatt gcttgctgcg cgcgccgtaa
     9301 aaacctttgg cgagggccaa gcgccccttg cggcgtttgc gactggccgg ggaattggtg
     9361 actctgggca tggattcttc tccttacact ttacgttgcg ctcacgcgcc caattcaatg
     9421 ccgagggact tggcaatctt gccgccggcc tgcacgttct ggcggaggcg ccgcttttgc
     9481 ttgcgggtct tgctggcggc caagtggcgc cgcccgggct tcttgtgcaa aatcttgccc
     9541 ttggccgtgc gcttgaaacg cttggcaacc gatttgcgcg ttttcttctt catgtttgca
     9601 acttcgtttc cgtaggttcc gggtcgccgc gcacggacaa cttgcccgcg gccgagaccc
     9661 tctggttcaa aacagacggc gacttgtacg ggatttgtcc cgcgccgtcc agtcaaatcc
     9721 tttgtttatc ttccgccgcc ggccggcgcg acgcgccccg gcgcctagcg cgaggccgcc
     9781 gccccctgct ggcgcgcctt gggattggga atcagcacca tcaggatatt gcggcccatc
     9841 agcttgggcg cctgctccac cacgcccacc tcgcgcaggt cggccagcac gcggttcatc
     9901 agcacaaagc ccagttcctt gtgggcattt tcgcggccac ggaagaacaa cgccaccttg
     9961 acgcgatggc cgtcctccag aaactcccgc atccggcgca gcttgacttg ataatcgttg
    10021 tcgcccacgg aggggtgaag ctggacttcc ttcaccttga cggcggcagc gttccggcgg
    10081 ttttgttttt cttttttgct cttctcgtac tgaaacttgc cgtaatccat gatccggcac
    10141 accggcgggc gggcggtggg cgagatctcg accaagtcga gaccggcggc ctgcgcgcgg
    10201 gcctgggcgt ccgcggtggc gaccacgccg agttgttcgc cgccgggccc gatgaggaat
    10261 acttcgggaa cgcgaatgcg atgattaatg cgagtgggga atccgccgcg cgggtcccgg
    10321 gggttggtcg ggggaggtat ggccattcta tccttgaggt tgggagccgt gcgccgctcc
    10381 gttcgcgttg gtggtgggca gcgccacttg ccgctccgcg tccaccttgg cgatgaacgc
    10441 atccagcggc atcacgccca gatccccagc cacccggctg cggaccgaca ctgtgccttg
    10501 ttccacttcc cgcttgccca gcacgagcat gtacgggatt ttttgcaatt gtgcgtcgcg
    10561 gattttggcg ccgagctttt cgctgcgctc atccaccacg gcgcgaatgt cacgcgcctt
    10621 gagctgcgcg gccaccgcgc gggcggcatc ggcctgcgcg tcggtcacgg ggatcaagcc
    10681 cacctgcacc ggcgccagcc aaagcggaaa cgcgccggca taatgttcaa tgagaatgcc
    10741 gaagaaccgt tcgaggctgc ccagaagcgc gcggtgcacc atgaaggggc ggtgttcctg
    10801 gccatcgggg ccgatgtagg ccatgtcgaa gcgctcgggc agattgaagt cgaactgcac
    10861 ggtgctcatc tgccaggaac ggccgatggc gtcgcgggtc ttgagatcaa tcttggggcc
    10921 gtagaacgcg ccgccgcctt cgtcgagctc gtaggcaatg ccctcggcct gcaacgcggc
    10981 ctccagcgaa cgctgcgcaa gctcccagcg ggcaggatcg cccacggctt tttcggggcg
    11041 cgtggcgaga taggcagtga tgttctcgaa accgaacgcg cgccacatgg cgatggcgaa
    11101 gcgcacgcat tcgcgcactt cgctctccac ctgttcttcc gtgcaataga tgtgggcatc
    11161 gtcctgggtg aagccgcgca cgcgcatcag gccatgcaac acgccggcgc gctcgtagcg
    11221 atagaccgtg cccagctccg cccagcgcag cggcagctcg cgataagagc gcttgtgcgc
    11281 ttggtaaatc tggatgtgga acgggcagtt catcggcttg atgtaatagc gctgctcgtc
    11341 aatttccatc ggcgcgtaca tgttctcgct gtagaacccg agatggcccg aggtttccca
    11401 caggcccgcg cgaccgacgt gcggggtgta gagcatttcg tagccggcgc ggtaatgctc
    11461 ccgccgccag aactcttcaa tgatggtgcg aatgcgcgcg cccttgggat gccaatgaat
    11521 caggcctggg cccaccgctt cctgaatgct gaacaaatcg agatcgcgcc cgagggtgcg
    11581 gtggtcacgc agcttggcct cggcaagcat cttcaaatgc gcgtccaaat cagcctgggt
    11641 caagaacgcg gtgccgtaga ggcgctgcat catctggttt ttctcgtcgc cacggaaata
    11701 ggcgccggcc acggacagca gcttgaacgc cttgatgtcg cccgtgcggg ccacgtgcgg
    11761 cccgcggcac aaatcggtga aatcgccgct gtgatagagg ctgatggttt cgccatcggg
    11821 aatatcggcg aggcgctcga gtttatactt ctggccgcgc tcagtcatca tctgccacac
    11881 ctcggcgcgc gatttttctt cgcgcacgaa gggctggttt tcggcgacaa tcttcgccat
    11941 ttccgcttca atggcgggaa aatcctccgc cgtcaggcgg tgggccagat cgaaatcgta
    12001 atagaagccg tcttcggtgt cggggccgat gtcaaacagc gcatccggcc agaggcggcg
    12061 gacggccgcc gccatcacgt gcgccgtact gtggcgcatg gtttccaacg caacttgctc
    12121 aaccttcatg aatactggat aatgtccttc ctgaaacgcg gcaacctacc ccgccccccc
    12181 ggcaaagtca atgcacccct tggcttcatg agtgggctgg cggcgggttt tccgccggcg
    12241 aggcaaacgg cgccacgacc tgtacggcgg gaaacaacgc acgcacctgc gactccgcgg
    12301 cgcgggtgaa aatcagcttc acgttgggcc cggcatccat cgtgaaccac aggggcaggc
    12361 catcgcggcg ggcggcccaa atggcgtgca gcgcggtcat ggtttcgggc agcgcataga
    12421 ccagcggcgg gcgagtggct gccatcagcg cgtgcatggc aagggcgttg ccttcggcca
    12481 cggtgcccag cgcggcgatg tcccgcgcag cgatggcggt tttcaactcc gccaaatccc
    12541 gcgcgacgcg cgcgggccat tcgcgataaa attcgcaagt cgccacgctg cggcgcatac
    12601 cctcgcgcga gcccacgggc ttttcggcag cgcataggac cagcgcgccc acacgcagct
    12661 ccggccacgt ggcctccagc ggcgcggcaa aggaatccat gccatcgggc gcggcaccgg
    12721 cgtgccattc cacaaagcca tcggccagac tgcgcgccgc gctgccactg cccagccgcg
    12781 ccaaaatgga ttgctcgcgc ggcggcaaat cccacccgaa gagcgcattc agcgccttca
    12841 ccagcgccgc gaaaccggag gccgacgacg caaaccccgc cgccgtgggc acggtgttgc
    12901 gcgcgcgcaa atcaaatgtg aaatccggcg cagggcggaa ccaatcgagg aatgcgctgg
    12961 cgcgccgcgc aaaggaactc tccgccggta tcggttcccc gttcagccaa atggcatccg
    13021 ccgcgccggc gccgcgcgcg agttccacat gcgaacccag cggccccagc gaaatggaca
    13081 agctgttcgt gaccggcaaa ttcagcgctt catcgcgctt gccccaatat ttcaccagcg
    13141 cgatgttggc cggcgcatac gcattggcca cggcgcgcgg cgtgctgccg cgcccggcca
    13201 aaatgcgggc caccacgtcg cgcggcgtca tggcaaagcc tccgccagca cgccggcagg
    13261 cgtcacttgc acagctagcg cgggcacgcc ccactccgtg cgcatggcct tgcccagccc
    13321 caccacgcaa tcgcccagtc cggagccgga aatcttgctg ccgtggattt tgggatcggc
    13381 gcgcagctca tagaccagcg ccgccaactt cgcgttgttc accccgagcg catccatcag
    13441 cccctgatta atgttcatca gcgcgcccat ggccggccag tcgccgcggg cggcggcttc
    13501 gaacgcgcgg ttgacggcat cgccaatcag gtcgtcgagc cgcgcgtgca gctccggctg
    13561 cgccttgcgc tgcgcctcca ccacggcaat cacttcgggt gtgggcattt tgctgccgga
    13621 atacagcacg gtcagttgcg gcgcagtggg cagttggacc acctcggtgg attccgcgtg
    13681 aaaccgcagg cagccgccat aggcgctcgc ggcgacatcc gcgccggaac ccacgccgcc
    13741 ctgcgcgcgg cgcaccacct gaatggccgg caccaccaat tcctccggcg caatggacaa
    13801 gccttgcgcg tggcgcagcg cggccagcac ggccagcgtc accgccgcgc tggtgcccag
    13861 ccccatcacg tgcgacattt cggacctgat atccagttca aacccctgcg ccagcccttc
    13921 gcgcaacgcg cggatggcgc ccaaggcgaa acgaaaacta tcgtgcggcg ccagctcgcc
    13981 cagcgtggtt tcgtgctcac ccagcgcgga gtgcaagacg atggtttcat cggtgcgtgg
    14041 cgtcagtttg accgtcagcc gcttgctgac ggcgcaaacc agcgcgggct ggccacggag
    14101 cacggcatgt tcgcccatga gcatgaggct gccgggcgcg gaggctttga cggcgcgcag
    14161 ggtcatgcag gaaacatcaa accacccgca cgcccagggg atcggggcgc acagtcagca
    14221 tttcgtagcc gaacttggcg cagaggtcgg ccggcgcttc gcgcgccgcc accagcacca
    14281 tgccggcctg ctcccccgtc accgcacccg ccccgcaaat cttggccgcg ccgccggact
    14341 cttccacggc ggccacaaat tcggccacgc gatccggcac cacgccgatg gtgttgagca
    14401 gccggtgatt atcgcgcatg gcgcattgga aatccttgag gttatcctgt tccagcgcgc
    14461 gctgcatttg gttggccacg gtttcaaaat cgtcccacac ccggacctgg gcgccgaggc
    14521 ggcgggcgac atcgctgacg gcctcgcccg tggtgctggc cggcgtgccg gtttgcacga
    14581 tatagagcgg catccgcggc agcggcagct cctgcgccgc gccttgctgg aagcgcacgc
    14641 agccgccgtg cagcgagaca taggtatcca gcccgctggc cttgccgtgc tgcatgtttt
    14701 ctacttcaag gctgtagcgt gcgtaccatt ccggacggaa atccacccgg taatagtggc
    14761 ccagaccgcg cagcacactc aaaatggccg ccgccgaaga acccatgccg cagccaatcg
    14821 ggatgtttga gttcagccgc acattgacgc ccccccccat cttcaggtgc aggccgtcga
    14881 gcaccgtcac aaacgcgtat tggaacaaat ccaccggttt gagcagaact tcgcggatgg
    14941 acagtttgcc ggcgagaaat tcctcataat tgcgccgcac ccgcgcgtga aaatcacgca
    15001 gcgcacgcag ggtgaaggat tctttcggag ccgtcacgtt cggcaaatcg aatgaaacgg
    15061 tctccgccgc cccgtccatc gtcacgaaga cttgggcgtt gcggttcacc gccatggcca
    15121 gcgccgggcg gccatagacc accgcgtgtt cgccggtcaa aatcaacttg cctggagcaa
    15181 ctgctttcac ttcaaacctc aagcctatcc cttcgcgcag gtaatgtaaa gcgttacggc
    15241 cgcgtttttc gcgagtgttt tcgcgctttt ggctgaccat ccccgcgcgt cacacccgcg
    15301 ctttcgcccc tccttggctc atgagccctt tccccaaacc cggacggggt ggaagccgcc
    15361 tctccagaaa cagacacagc ccacgacgac gccaatctat ccggcaaaga cggtattacg
    15421 atctcgaatc attgggcaat tgattttccg tcggcacttc atgcaacagc ctctcgatga
    15481 ctggatagga cgagggatcg ttggggttgg ccggcagcgc tcggatgatt tcaatcaatg
    15541 ccttgccgcc ttcgccggta taagtggcga tgaagctgcg aaacgtttcc gacgcggttt
    15601 cgtcggcatg gtcactgatg attttccaag cgaacaacgg cacgccatgg tcggcgcaaa
    15661 ccagcgccag cccaaagctg ttcatgtcca cggcgtccgc ctgagtcatc gcctgcaacc
    15721 ggtcgcgctc gcgggtcgtg gagatgaact gttcgccaga ggccaccgca atagtggatg
    15781 ttgtctgcaa gggagaggga aaaactgcca ccggcaagcg cgtccaatct gtacgccatg
    15841 tcttggcctg actgtcggtc tgccaagccg taacgcttcc cacccggtgc cactggtgag
    15901 tcgccatctc atcgctcaac gcacccgctg gtccgatgga aaatgcccag tcgcaccgaa
    15961 agcgtgacag tagcgcttgg acggagaccg ccgtttccac cgccccggag cccatcttca
    16021 ccgcataaat ggtgtgggca tccaactgga gccgttggac ggaacgcgac cccacacgag
    16081 tcgcctgccc catttcccgg gcttgggttt tcaacgcctg caaatcggcg tcgagggcat
    16141 aaaaaaaacc gatggtatcg cctggggcct gcgtaacgca aaacgccagc aagccgaaaa
    16201 acagcctaat ccttttgcgc atggttgaaa attcctccta ccgttgattg gcgattcagc
    16261 atgggaacga ttccaaaagc aaacccaaag tgcaggaatt acaacaagac ccgtccccat
    16321 tgtcgttaat gataattcaa cacaaaaata cttgcgccgg atattggcga gacgcacgat
    16381 ggtggccaat gaaagcgatc agtcgcagac agttcttgca gaccgccgga gtcgccatgc
    16441 tggtggccac gctgccgg
//
LOCUS       DAOPNP010000051        16211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4273_length_16211_cov_8.533734, whole genome shotgun sequence.
ACCESSION   DAOPNP010000051 DAOPNP010000000
VERSION     DAOPNP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4273_length_16211_cov_8.533734"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKI64_06110"
     CDS             <1..476
                     /locus_tag="PKI64_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00621.1"
                     /translation="IGACVVNKAAWQRVEQAKYVGTWFAHVGVIMEAAHGHDIPMLAE
                     PLILNRCGTTEVFTWTSTMMDVLDGWRRLCVLLEPLYGASACRESLHSFRRAHGLGKL
                     KFLAYARAGGALTPAVVREHILPGNESAWFKWWAQIVARLPAGWFRTLQRWKHHA"
     gene            473..2221
                     /locus_tag="PKI64_06115"
     CDS             473..2221
                     /locus_tag="PKI64_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00622.1"
                     /translation="MNLLGQASLISGIQVLLYVLIPAWVLCRWQWRDFGEGVFAFLLA
                     GVCSVALLGRVGNAGGWGVPVVLPIWLAFWLGWGIWVWWRKYPVVSLPWDGLLLAILL
                     LAWVVRMIHPLQTWALGQSDAYSHLEFLQNVLTAGRVLQPDYPPAYAWVQALPAWLLQ
                     GHPYWLARFGGALFGVGLVLGCYALWAGIRYRVAGVAAAALVAGCPFFFLLQKTGVGC
                     FANQLGLMLIPAALWAYLTGRRGWLLVALATLAVAVPMMLLHVLLLLCGLLLVEREKG
                     LGRFRLLVWLGLALLLVIGLALQLPPLRGRVIVAMLTGQYELIDVADLGWPEIFRLLW
                     TDFWGIKRLGYSSVLLNGMAAGSLLVFVSALAWGWRQRDAAWRLVGVWGLLTSINVHL
                     GCMQFTNYQREGWSLLLVVAGLCALLFAEVWQRVNKRALRGALGASLTIAALAGLLFP
                     PAHVPLGGETESDVVRYLVNADPEITILTRNMSSFSYDQGDIARTLHPKAVRSVADLP
                     IGGTAIFLRDYPPAEPTVSRVMQLLQPTLTAECQRAQRMAEEANQQLEQQLAGYVLSK
                     ETYSPRMEIWHVRAKH"
     gene            2232..4511
                     /locus_tag="PKI64_06120"
     CDS             2232..4511
                     /locus_tag="PKI64_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00623.1"
                     /translation="MAWLMHLFAAATLLPLLVLLARQGPAVVTAWRVASRVARSSLII
                     LWLILAGSLLLRPHEDVFTGLDNMAYRLMPKVLLGGRGFHSQDVVLRDVPEPLREIFL
                     YRPGPLGRATRDMVFQLSGWNSTDTAPFFMPTLPLAAAGVEPWLKSDQFVPLVGAIWA
                     ALLLAAGFGAGGGWGLLAAGTLWVTTAWPAWFLRGFYGEGVGAVLISSVMLSLGRQPR
                     NFGLLLMLGFMLGLALCFHPTLAVLAAPVLLILLIEEHAWKKVAGALAGALAGAFPFW
                     AMTRWVCQPNGDWTHWHNLQRLLRVAEIQAMAWALGLMVVLALILVLLSRVPRVAEWA
                     QHWDSRLTPWGWLVIAALPVIAVWAAPGHIGDVLRLGAQATWSGMRWSGTLIILLCTI
                     FLIRPSRPWRERVWFVLLCWVALMFLFIKGEETPVGLWSQRRFLPVVLVGLGLCLVPL
                     AEGLKTMAMRGLWRAKVLAAILVVAGLWNLVQWPAPYYVVNEQGATAWTEDIAQTIGT
                     NRWVIFDYFPHSVPYASNLKHRILGIGERTVSRWDEVAEWMASLAQTSEVWLASSWSL
                     CSLEEGVRIEPLNVRTAAFPIVRAKAFFPAEVGTRELQHDFGRVMPLQPNDIPVQVKA
                     LDGSPVGLRGPWHALRNGGAWSRQGSGVIGPIPPAGGAVVFEVAGEWPAPTVDWPEQV
                     LLVLPPWGGEPLRLTVTTGALQTVRGVLTRPATDAERPLTGVYTCHTERPYNPQEYGM
                     RGYPPDLGLVLRQVIIRIE"
     gene            4504..5307
                     /locus_tag="PKI64_06125"
     CDS             4504..5307
                     /locus_tag="PKI64_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE00624.1"
                     /translation="MNSPVNPCQTSPSMDPASQSFLQRTMVLIPAYNEEASLPALLAE
                     VHRELPEAAVTVIDDGSADQTSRVAREGGARLLRLPCNMGVGPAVQTGFVYALECGFE
                     HVIRLDADGQHPPAEARKLVATMLTAPADLVVGTRYGAGSLYHGSWSRQIVLKGLALF
                     VSLICRQRITDPTSGFWLLSRPLLQCFALHYPSDYPEAEAIALLRRQGFSMAEVPVVF
                     RPRVAGQSSIHAWNSFIFAAKVFLALFVDRLRAVDPRGSAEFIKKAGAT"
     gene            5406..5774
                     /locus_tag="PKI64_06130"
     CDS             5406..5774
                     /locus_tag="PKI64_06130"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOE00625.1"
                     /translation="MGAVSLGVFLITILAIRSYKLREKYAVLWLMTAAMITLVALFPH
                     LVDVTREWFGMQYVTSVVAVIFIFLVLVSFHFSIALSALNDKAAAIASRCAMLEARIQ
                     ELEQKISVVRSNHPNERIKP"
     gene            5771..5929
                     /locus_tag="PKI64_06135"
     CDS             5771..5929
                     /locus_tag="PKI64_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00626.1"
                     /translation="MMLAYIDPGSGSILLQVVLASMIGGVAMFWNKIKSLFVRKKPTQ
                     PEPPKSEP"
     gene            6008..7351
                     /locus_tag="PKI64_06140"
     CDS             6008..7351
                     /locus_tag="PKI64_06140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE00627.1"
                     /translation="MDQTCAALLRDLDVQGHLARWNREGLIVASRFVDDPELIATLTA
                     AHPGYPAFLEHDRIEPITYPYEWSTSMLADAGRLTLRLQSELLDLGLSLKDATAYNIQ
                     FVDGRPVFIDLSSIERPARLDMWFALGQFNRMFLYPLLLVKHAGWDLRSYFLPSLDGR
                     STLQVGQAFSWWRRWSPGLIWDVGLPYALEGRVKSRKPSAPSTPSADATTTPVGLPTA
                     QRLTLRRLEKKIARLAAAIAPKTVWSDYTQTCSYDAQAEAAKKSLVRQFLEEMRPATV
                     LDIGCNTGDYSRIAASCGAKVLATDFDVGAVEQMYQRLRSEPAAITPLVVDIANPSPG
                     IGFLNAERPPFLERAKPDCILALAVIHHLLVGANLPMAAIRDLLASLTSQHLILEFVP
                     TDDVMFRKLLEFRMNLFAHVTLGYCCEVFGERFTLQRQESIEHSPRTLLWFTKRA"
     gene            complement(7394..7705)
                     /locus_tag="PKI64_06145"
     CDS             complement(7394..7705)
                     /locus_tag="PKI64_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00628.1"
                     /translation="MRRRPISHGFLYDGTAGKVQTEAGWDGQTQFNADGSARAIEIWR
                     SEHDSGRDGWSTEGNQLMLNVGEYHWTGTYTVRGNQFTLSNVPNYDHKGHTGAFIFTR
                     I"
     gene            7824..8945
                     /locus_tag="PKI64_06150"
     CDS             7824..8945
                     /locus_tag="PKI64_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOE00629.1"
                     /translation="MSALRLGINTLFYLPGEVGGSETYLRETLRALTQRSEAPQLVLF
                     TNAENHDLLQREFPSAECIPTGIAATNRFRRIWSEQTALPRLIRRAQPDVLWSPGYTA
                     PALTGVPQVVSILDMQYKRFPQDVTWLAARTMDVLYPMAARRSTAIVTISEFAKTEII
                     RFLRVPADKITVTLLGASAEYGAASGLPPEMVRGAPYLLCVAHTYPHKNVAMLVRAFT
                     RLAAKIPHRLVLVGKARLGEPEVAEALRQAPADRVVRLAEVSAADLASLYQCADWLVF
                     PSLYEGFGLPVLEAMRAGTPVLTTREAAIPEVGGDAVVYADGRNEEAWAKTMRTVLTW
                     PREMRLRLTVAAKQRAAGFTWKASAEQLWAVLTRAARKG"
     gene            8950..9639
                     /locus_tag="PKI64_06155"
     CDS             8950..9639
                     /locus_tag="PKI64_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HOE00630.1"
                     /translation="MTTTLTIIALSLLAGGLFAGLLLVLRRPRGRGMSVRTVAGIEDL
                     KAIGVLSVFKAVTKEIVTARDHSLGNLGKRYLEWLITSRKMAMIFEFDIDFQYDLRDP
                     GFTVREEAPGRYRLQMPRCEYVVHIRNVTFYDEQGGKLLPIIIPDVINKILGGGFNEE
                     DRNKLMDEAREQAEKLAQNLVLRLNSEVQSSARQTMEMLAKSFGAQQVAVDFSNAEPR
                     KTQLVDSAAVR"
     gene            complement(9642..12566)
                     /locus_tag="PKI64_06160"
     CDS             complement(9642..12566)
                     /locus_tag="PKI64_06160"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOE00631.1"
                     /translation="MKKTLRSFWRWILLLAMAAGAVTLWRSDRWLFEWPAEKSQHYFE
                     GVGHRLAKHHGLAFLMTFDEAHPVEWIARGTVLYPGTTRVPGRLGFARQFDGKARTML
                     ETTAIWPALGSNYTLSLWVKVEPACHDQEIWYTFIQGRYTGFKLQNGQMMFFVPGSAE
                     SPAAHYPFTAYGKFVHLAGVVDAAQGTAQLYENGTLKATVPFADVSHPTQNIEFGKTR
                     MYAVTAPFCGAIDEAAAWTRSLSSREIHRLAHAHRSLPRALEPFAWFRWRMYAMWQAA
                     LPATLNLLTRFNPLLHEGRLATLPTIELRFSSKDARHFRNTHEASLASGRRTDEGAKW
                     RRIYALHASQTAEAHLQLDGSTASYPIAKRPGYILELPPESPIFGARTLRLVPPENLA
                     DRLPTLAQAVLATTPTNHAAGPKLCRLLIDGQSKGAYVAIPFAQRGVGPGERTWVADG
                     ARNPNDWTALFRGAKHQPDVPTLTAEQLSTGLREARILLAHDLFNPWSPREMAWRFRP
                     WQEADDMPEILPLTSFTILGNNPAPFYIVEDLDLAAVRWAGKKLTWHSSRPNIVDNQG
                     HVTRPAGDVPVDVKLTATLGTGKTRQKQTLAFRVIPQTPRLAAIHLSVAEALSATHRV
                     EFSARFDPAGGGEPQWLQGGQASGGGIKHRGNTSYWRGRRKPFSLRFDTSQSILGAGN
                     SPYLLFLNGYADATKLKNKLAYDFFRACATPDQPRFAPELEWCEVFFNGAYFGIYEMC
                     TRVDENMTGLAPVVPDNPGGAIFKIRPSPHLFQQPEPEAADQIFPSPSRADRRADLTG
                     LLTFTSQSSPDDFKHNIAAHVDLANAIDFLLLLNLAQNVDGRTTNFYLARGDEPAARF
                     FFIPWDYDHTFEKTGRWLSNYLFDRLWQEMPDFSAQTGQRWRELRHGIWSDAALQEWI
                     ASMSAHLADYMAWDDAVMEREDLPKYSVRIEQFRQAVLESAHALDERLAIIAPPQAPP
                     AE"
     gene            complement(12563..14323)
                     /locus_tag="PKI64_06165"
     CDS             complement(12563..14323)
                     /locus_tag="PKI64_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOE00632.1"
                     /translation="MTFPPPAPAAIAAQQNPPATPEWAKDAVWYQIFPERFRNGARQS
                     NPRPEDIGEVPFTGWRVSPWGMDWYARDDWEQPHAANFWTTVFQRRFGGDLVGVREQL
                     PYLQQLGVNALYFNPIFWAPSLHKYDAIALHHIDPTLGPDRDGDLRALAAANETEDPA
                     TWIWTAADRYFLDLVAEAHARGMKVILDGVFNHSGTGCFAFQDVRRLGPKSRYADWYQ
                     ISEWRADGTFAYASWDGAGTLPNFGRDAETLNPGIKQYLFAITRRWMDPSGAGWPAEG
                     VDGWRLDVAYCVPHGFWREWHAYARGINPEVYTTAEIVGPAQDWLRPDEFSAVMNYEW
                     TYPTLSFFTPHPQAIGAAEFQQRINTLHARHEPGTVHILQNLLDSHDTGRVLTMLEST
                     CPPFSGWDEYFHWAKAANNPGLVTRQPGTRAKQALKLAVAWQFAGPGAPMIYYGTEVG
                     MWGANDPCCRQPMLWSDIVYDDEAHGYHGPLPQATPRRPDGELLAYYQRWIALRHAES
                     ALRRGSFRWLPTPNEHTVIFARELGDTAIVCAINKGDHATALPAWPGARDLWHGKPAP
                     TAVKPHSFVLLRLPTGVFSQ"
     gene            complement(14320..14799)
                     /locus_tag="PKI64_06170"
     CDS             complement(14320..14799)
                     /locus_tag="PKI64_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOE00633.1"
                     /translation="MNMPNVRVFDADFLDRLTAEAAATPRRRKNFNLHASDDYPCHRF
                     FNAMCADTYVAPHRHADAAKDESLVLLRGTLGVVIFDEQGQVVLARRLQAGQVADVPH
                     NTYHAWVALEDGTVFFEAKAGPYVPLRPIEKAPFAPPEGDPRAPEYLAWLKSLCREP"
     gene            complement(14861..>16211)
                     /locus_tag="PKI64_06175"
     CDS             complement(14861..>16211)
                     /locus_tag="PKI64_06175"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5116 domain-containing protein"
                     /protein_id="HOE00634.1"
                     /translation="CGKWAGALLAALLCATPAPAQTNPFHAPLYWSVYENLFRKEQAG
                     QPEPYITEAEWLANINWVEANLKPFGYDMVCVDGWGDVSRLNQNGYRLTHSRHWEHDY
                     AWWAAYLRERGLAMGMYGNPLWVHVNDDDTERRIVGTDILVASLKDPAEDARFPWVQV
                     ERPGAEQYVKGWIHFYADMGIRFFRIDFLSWYEDGQDRWLGRVGPARPRAHYETALRW
                     MREAADERGMLLSFVMPHLNHDAAAERRYGHMMRVNEDTGEGGWNKWSEWFRGQKREG
                     WSVYGNAADGLAYWSRFAGRGRIILDPDFIRLNTFATDDEKRSVIAQCLLAGAPIAVA
                     DQHDTIGDNLRFYTNNELLELNRDGFVGQPLSWDPTQEESQIWFGQLSGGDWVIGLFN
                     RENEPRSRRLNFAALGLAEAARVRDLWTHEDLGEMESFAAEIPPHGCRVLRLSPPQE"
BASE COUNT         3046 a         4513 c         5103 g         3549 t
ORIGIN      
        1 tcatcggcgc ttgcgtggtg aacaaagcgg cctggcaacg ggtggagcag gccaaatatg
       61 tggggacatg gttcgcgcac gtgggggtga tcatggaggc agcgcatggg cacgacattc
      121 ccatgctggc cgaaccgctg attctcaacc gttgcggtac gacggaagtg ttcacgtgga
      181 cctcgaccat gatggatgta ctggatgggt ggcggcgctt gtgtgtattg cttgaaccac
      241 tgtatggcgc gtcagcttgc cgggaatcac tgcattcttt tcgccgcgcg cacggattag
      301 gcaaactgaa gttcttggct tacgcccgtg caggcggtgc gctcacgccg gcggtggtgc
      361 gcgaacatat tttgccgggc aatgagtcgg cttggttcaa atggtgggcg cagatcgtgg
      421 cccgcctgcc cgcaggttgg tttcgtacgc tgcaacggtg gaagcatcac gcatgaatct
      481 gttgggtcaa gcgtcgctga ttagcggcat tcaggtgctt ttgtatgtgt tgatccctgc
      541 ttgggtactg tgtcgttggc agtggcgcga ctttggggaa ggggtgtttg cttttttgct
      601 ggccggagtg tgttcggttg cgctgctggg acgggtgggt aacgccggtg gatggggggt
      661 gcccgtggtg ttgcccatct ggctggcatt ttggctgggg tgggggatat gggtgtggtg
      721 gcggaaatat ccggtggtgt cattgccgtg ggatgggttg ctgctggcga ttctgttgtt
      781 ggcatgggtt gtgcgaatga ttcatccgct gcagacctgg gcattggggc aatcggatgc
      841 atacagccat ttggagtttt tgcagaatgt gttgacagca gggcgcgttc tgcagcctga
      901 ctacccgcca gcctacgcct gggtgcaggc tctccccgca tggctgctgc aagggcaccc
      961 ctactggctg gcgcgttttg gcggggcgtt gttcggcgtg ggcttggtgt tgggctgtta
     1021 cgcgctgtgg gcaggaattc gatatcgcgt ggccggagtc gcggcggcgg ccttggtggc
     1081 tggctgtccg ttctttttcc tgctgcaaaa aaccggggtg ggttgttttg ccaatcaact
     1141 cgggcttatg ttgattccag ccgcactatg ggcctatttg acgggaaggc gcggctggct
     1201 gctggttgct ctagccacat tggctgtggc cgtacccatg atgctgttgc atgttctgtt
     1261 gcttttgtgt gggctgttgt tggtggagcg ggaaaaaggc ctcggccgat tccggctcct
     1321 ggtttggttg gggttggcgt tgctcttggt gatcgggcta gccttgcagt tgccgccgtt
     1381 gcgcgggcgg gtcattgtgg cgatgctgac cggacaatat gagcttattg acgtcgctga
     1441 tctcggctgg cctgaaatat ttcgcctgct gtggactgat ttttggggca tcaagcggct
     1501 tggctattca tctgtgctgt tgaatggcat ggcggcggga tcgttattag tctttgtgtc
     1561 ggcgttggca tggggctggc ggcaacggga tgccgcctgg cggttggttg gcgtatgggg
     1621 attgttgacc agcatcaatg tgcaccttgg atgcatgcag ttcaccaatt atcagcgcga
     1681 aggctggagt ttgctgcttg tggtagcagg actatgcgcc ttgctttttg ctgaagtctg
     1741 gcagcgcgtg aataaacgcg ctttgcgtgg tgcgctgggg gcgtcactga cgattgccgc
     1801 tctggccggt ttgttgttcc cgccggccca cgtccccttg ggcggagaaa cggaatcgga
     1861 tgtcgttcgc tatttggtga atgccgatcc ggagatcacc attctaactc gcaacatgtc
     1921 gagtttctcc tatgaccaag gcgatattgc gcgcacattg caccccaagg cagtgcgcag
     1981 cgtggccgat ttgcccattg gcggcaccgc aatatttttg cgggattacc cgccggcgga
     2041 accgaccgtg tcgcgggtca tgcaactcct ccagcccacg ctcaccgcgg aatgccagcg
     2101 ggctcaacgt atggccgagg aggcgaatca gcagctcgaa caacagctgg caggctacgt
     2161 attgagcaaa gaaacctatt cgccgcgaat ggaaatctgg cacgttagag ccaaacacta
     2221 ggagacccac gatggcttgg cttatgcatc tatttgctgc ggcaacgctc ttaccgctgt
     2281 tggttttact ggcacggcaa gggccggccg tggttaccgc gtggcgcgtg gcgagtaggg
     2341 tggcgcgcag cagtctgatt atcctgtggc tgattttggc tggtagtttg ctgctgcgcc
     2401 cgcatgagga tgtgtttacc ggactggaca atatggccta ccgcctgatg ccaaaggtgc
     2461 tattgggtgg gcgagggttt catagtcagg atgtagtgtt acgcgacgtg ccggagccgc
     2521 tgcgcgagat atttttatat cgtcctggac cgctgggacg agcaacgcgg gatatggtgt
     2581 ttcagttgtc ggggtggaat agtaccgaca ccgcgccatt ttttatgccg acgttgccac
     2641 ttgccgcggc tggtgttgaa ccgtggttga agtcggacca gtttgtgccg ttggtaggcg
     2701 caatttgggc tgcgttgctg ttggcagcag gctttggcgc agggggaggg tggggcttgc
     2761 tggcggccgg gacgctatgg gtgacgactg catggccggc gtggtttttg cgcggcttct
     2821 acggagaggg cgtgggggcc gtgctgattt ccagtgtgat gctctcattg ggacgccagc
     2881 cacgtaattt tggtttgctt ttgatgctgg gcttcatgtt ggggctggcg ctttgttttc
     2941 atcccacatt ggcggtgctg gctgcacccg tgctgttgat tttgctgatt gaagaacacg
     3001 catggaaaaa agtggccggt gcgctagctg gtgcgctggc gggcgcgttc ccattctggg
     3061 ccatgacccg ttgggtatgt caaccgaatg gcgattggac gcactggcat aatttgcagc
     3121 ggctgctgcg cgttgcagaa atccaagcaa tggcgtgggc gctgggccta atggttgttt
     3181 tggcgttgat tctggtgttg ttaagccggg taccgcgggt cgctgaatgg gcgcagcatt
     3241 gggatagtcg attaactcct tggggttggt tagtgattgc ggcgttgcca gtgattgccg
     3301 tgtgggctgc gcccggtcat attggcgatg tattgcgttt gggtgcacaa gccacgtgga
     3361 gcgggatgcg gtggtcgggt accttgatta ttttactatg cacgatcttc ctgatacgac
     3421 catcgcgtcc ttggcgcgag cgggtctggt tcgtgctcct gtgctgggtg gcgctgatgt
     3481 ttctcttcat caagggcgag gaaaccccag ttgggttgtg gagtcagcgg cgctttctgc
     3541 cggtggtatt ggtggggctg ggcttgtgct tggtgcctct ggcggaagga ttgaaaacaa
     3601 tggcgatgcg cggtctgtgg cgggccaaag tgttggccgc cattttggtc gtggcgggtt
     3661 tgtggaatct ggtgcaatgg ccggcgccct attacgtggt caatgagcaa ggcgcaaccg
     3721 catggacaga ggacattgca caaaccatag ggaccaaccg gtgggtcatt tttgattatt
     3781 ttccacatag cgtaccctat gcatctaact tgaaacaccg gatactggga attggtgaga
     3841 gaaccgtctc gcgttgggat gaagttgcgg aatggatggc ttcattggcc caaacatccg
     3901 aggtgtggct ggcttcctcg tggtcgctgt gttcgttgga agagggagtg cgcattgagc
     3961 ccctgaacgt cagaaccgcc gctttcccaa tcgtgcgcgc caaggccttt ttcccggcgg
     4021 aggtcggcac tcgcgaattg cagcatgact ttgggcgtgt gatgccactg cagccgaatg
     4081 acattccggt acaggtcaaa gcactggatg gcagcccggt tggtttgcgt gggccgtggc
     4141 atgcactgcg caatggtggt gcctggtccc ggcaaggcag cggggtgatt ggaccgattc
     4201 cgccggctgg cggtgcggtt gtgtttgagg tcgccggcga atggccggcg ccgactgtgg
     4261 attggccgga acaggtgttg ttggtattgc caccgtgggg cggcgaaccc ttgcggctca
     4321 cggtgacgac cggcgccctg caaacagtgc gcggcgtttt gacgcggcca gccaccgacg
     4381 ccgaacgacc tttgaccgga gtgtacacat gccacacaga gcggccttat aatccgcaag
     4441 aatacggtat gcgtggttac ccacccgatc tcggcttggt gctgcgacaa gtgatcattc
     4501 ggattgaata gccccgtcaa tccgtgccag acttccccct cgatggaccc agccagccaa
     4561 tcatttctgc aacgcaccat ggtgctgatt ccggcctata acgaggaagc gtctttgccg
     4621 gctttgttgg cggaagtgca ccgtgaattg ccggaggccg ccgtcaccgt gattgatgac
     4681 ggctcggctg accaaacctc gcgcgttgcg cgggagggcg gggcgcgcct gctgcgcctg
     4741 ccctgcaata tgggagtggg accggccgtg caaaccggtt ttgtctatgc gttggaatgt
     4801 ggatttgagc acgttattcg gctggatgcc gatggccagc atccgccggc ggaggcccgc
     4861 aaattagtgg cgaccatgct gacggcgccg gcagacttgg tggtcggtac gcgctacggc
     4921 gcgggcagtc tctaccacgg atcgtggtcg cggcagattg tactgaaggg attggcgttg
     4981 tttgtctcgc tgatttgccg tcagcgcatt accgacccca cctccgggtt ttggctgttg
     5041 tcgcgcccac tgctgcaatg cttcgcgctg cactatccat cagattaccc cgaagccgag
     5101 gccatcgcgc tgctgcgccg gcaggggttt tccatggccg aagtgccggt ggtcttccga
     5161 ccgcgcgtcg cggggcagtc gtccattcac gcgtggaatt cgtttatctt tgcggccaag
     5221 gtgttcttgg ctctgtttgt ggatcgtctg cgcgcggtgg acccgcgcgg ttcggccgaa
     5281 ttcatcaaga aagccggggc gacatgaaaa aaggcttgtt tgcttgtgtg gtattggcgg
     5341 tggctgcgtg gctttgctgg ccgtggctga ccaccgccag ccaaatcaac cgtatccgtt
     5401 ttctgatggg tgcggtcagc ttgggcgttt ttttgattac catcttggca atccggagct
     5461 acaagctgcg tgaaaaatat gccgtgctgt ggctgatgac cgcggcaatg atcacgcttg
     5521 tggcgctgtt tcctcacttg gtggatgtga cccgcgaatg gtttggcatg cagtacgtca
     5581 cctccgtggt ggcggtgatt tttatttttc tggtgctggt gtcgttccat ttttcaatcg
     5641 ctctgtcggc cctgaatgat aaagctgcgg ccattgcttc ccgttgcgcc atgctggagg
     5701 ctcggattca ggaattggaa caaaaaattt cggtggttcg aagcaaccac cctaatgaaa
     5761 ggatcaaacc atgatgctgg cctatattga ccctggcagt ggcagcatat tgctacaggt
     5821 ggtgcttgcg tccatgattg ggggcgtagc gatgttttgg aacaaaatca agtcgctctt
     5881 cgtccgcaaa aaaccgacgc aaccggaacc acctaaatcc gaaccctaac gcatgactcg
     5941 ggttgccggt tcatttcgag atcccagtgg ctacgtgttt gaacacgatg gtcgagtatt
     6001 tcgcgcgttg gatcaaactt gtgctgcgct cctgcgcgat ttggatgtcc agggacatct
     6061 ggcgcgttgg aaccgggaag gattgattgt cgcctcccgg tttgtggatg atccggagct
     6121 aatcgccacc ttgactgccg cccacccggg ttaccctgca tttttggagc acgaccggat
     6181 tgagcccatc acctatcctt atgagtggtc cacctccatg ctggcggacg ccgggcgact
     6241 gactctgcgt ttgcaaagtg aactgttgga tttgggcctg tcgctcaaag atgccaccgc
     6301 ctacaatata cagtttgttg atggacggcc ggttttcatt gatctttcat ccatcgaacg
     6361 tcctgcgcga ttggatatgt ggtttgcgct cggccagttc aaccgaatgt tcttgtatcc
     6421 gttgctgctg gtgaaacatg ccggttggga tttgcggtcc tattttttgc ccagcttgga
     6481 tggccgttcc accttacaag tgggtcaggc attcagttgg tggcgccgtt ggagccccgg
     6541 attgatttgg gatgtgggcc tgccgtacgc attggaaggc cgggtcaaat ccagaaaacc
     6601 ttctgcgccg tcgacaccgt ctgctgacgc caccaccacg cccgttggct tgccgaccgc
     6661 tcaacgcctg acgctgcgcc ggctggaaaa gaaaattgcc agactggctg ccgccatcgc
     6721 gccgaaaacc gtctggagtg attataccca aacctgttcc tacgacgcgc aggccgaagc
     6781 ggcaaagaaa tcgttggtgc gtcagttcct tgaagaaatg cggccagcca ccgtattgga
     6841 tattggttgc aacaccggcg attactcgcg cattgccgcc agttgcggag ccaaggtact
     6901 ggctaccgat ttcgatgttg gcgcggtgga gcagatgtat cagcggctgc ggtctgaacc
     6961 cgccgccatc acgccgctgg tggtggatat cgccaatccc agcccgggta ttggtttcct
     7021 gaacgcagaa cgcccaccgt ttttagaacg cgccaaaccg gattgcattc ttgcgttggc
     7081 ggtgatccat cacctgttgg ttggcgccaa cttgcccatg gcggccattc gcgatttgct
     7141 ggcctcccta accagccaac accttattct ggagttcgtg ccgaccgacg acgtgatgtt
     7201 ccgtaaacta ctggaattcc ggatgaattt gttcgcgcat gtcacgttgg ggtattgctg
     7261 cgaggttttt ggcgaacgct ttacgttgca acgccaagaa tccatcgagc attcaccacg
     7321 aacgctcttg tggttcacca agcgcgcctg aaagccggtg ctatttaccg ccacgcaaca
     7381 tgcgcgacac tgtttagatg cgtgtaaaaa taaatgcgcc ggtgtggcct ttgtggtcgt
     7441 agttgggcac gttggacaaa gtgaactggt tgccgcggac ggtgtaggtg cccgtccaat
     7501 ggtactcgcc tacattcagc atgagctgat tgccctcggt gctccaaccg tcacgacccg
     7561 aatcgtgttc gctccgccaa atctcgatgg cacgggccga accatccgcg ttgaattgcg
     7621 tctgcccgtc ccagccagct tcagtctgaa ccttgcccgc cgtgccgtca tacagaaatc
     7681 cgtgcgaaat agggcgtcga cgcacgattc ggcaatgccg agcatatctt gtcgggaggt
     7741 ggtcgggctg ctataaatga ccgtgatgat caggagccgc ggggtacgcg gcgagaccat
     7801 gtagccatct tgggcgagga ctgatgagtg cattgcggct agggatcaac acgctgtttt
     7861 atttgccggg cgaggtcggg ggctcggaaa cctatctgcg ggaaactctc cgcgcattga
     7921 cccaacggtc ggaggcgcca caactggtgt tattcaccaa tgcggaaaat cacgatttgt
     7981 tgcaacggga gttcccgtcc gccgagtgta tccccaccgg cattgcggcc accaaccgtt
     8041 tccgccgtat ctggagcgag caaaccgcgt tgccgcggtt gatccggcgt gcgcagccgg
     8101 acgtgctgtg gtcgccgggc tataccgcgc cggcgttgac cggcgtgccg caggtggtgt
     8161 ccatactgga catgcagtac aagcgtttcc cacaggatgt tacgtggctg gcggcgcgga
     8221 ccatggatgt gctgtatcca atggcggccc ggcgcagcac ggccatcgtg accatttccg
     8281 agtttgccaa aacagaaatc atccgctttt tgcgcgtgcc ggcggataaa atcaccgtga
     8341 ctctgctcgg agccagtgcg gagtatggcg ccgcgtccgg tttgccgccg gaaatggtgc
     8401 gtggcgcgcc ctatctgctg tgtgtggcgc atacctaccc gcacaaaaac gtggccatgc
     8461 tggtacgggc gttcacccgg ctggcggcca agattccgca tcggttggtc ttggtgggca
     8521 aagcgcgctt gggcgaaccg gaagtcgctg aggcgttgcg gcaggcgccg gcagaccgcg
     8581 ttgtgcggct ggcggaggtc tccgccgccg acctggcttc gctgtatcaa tgcgcggatt
     8641 ggctggtgtt cccgtcgctg tacgaaggtt tcggcctgcc cgtcctggaa gccatgcgcg
     8701 cgggtacgcc ggtgctaacc acccgcgaag ccgctattcc ggaagtcggc ggcgatgccg
     8761 tggtgtacgc ggatggccgc aacgaagaag catgggccaa aaccatgcgg acggtgctca
     8821 cgtggccgcg cgaaatgcgc ctgcggttga cggtggcggc caaacaacgt gcggcgggct
     8881 ttacctggaa agccagcgcg gagcaacttt gggcggtgct gacccgggcg gcacggaaag
     8941 gataacatca tgacaacaac cctcacgatc atcgcgttga gtttgctggc cggggggcta
     9001 ttcgcaggcc tgctgttggt gttgcggcgg ccgcgcggcc gcggaatgtc cgtccgcacg
     9061 gtggctggca tcgaggacct caaggccatc ggcgtgctct cggtcttcaa ggcggtgacc
     9121 aaggagattg tcacagcccg cgaccattcg ctgggcaatt tgggcaagcg ctatctggaa
     9181 tggctgatta cctcccgcaa aatggcgatg atttttgagt tcgatattga ttttcaatat
     9241 gacctgcggg acccgggctt caccgtgcgc gaggaggccc ctgggcggta tcgcctgcaa
     9301 atgccgcgtt gtgaatatgt ggtacacatc cgcaacgtga cgttctatga cgagcagggg
     9361 ggaaaattgc tgccaatcat catccccgat gtcatcaata aaatcctcgg cggcggtttc
     9421 aatgaagaag accgcaacaa actgatggac gaagcccggg agcaagccga aaagttggcg
     9481 caaaacctgg tgttgcgcct gaactccgag gtgcaaagct ccgcgcggca aaccatggaa
     9541 atgctggcca agagcttcgg cgcacaacag gtcgcagtgg acttctccaa cgcggaaccg
     9601 cgcaaaacac agttggtgga ctccgccgcc gtccgttgat gttactccgc cggcggcgct
     9661 tgcggcgggg cgatgatggc gagccgctca tccagcgcgt gcgccgactc cagcactgct
     9721 tggcggaact gttcaattcg caccgaatac ttcggcaaat cttcccgctc catgacggca
     9781 tcgtcccacg ccatgtaatc ggccaagtgg gcgctcatgc tggcaatcca ttcctgaagg
     9841 gcggcatcag accagatgcc gtggcgcaac tcgcgccacc gttgaccggt ttgcgccgaa
     9901 aaatccggca tttcctgcca gaggcgatcg aacaaatagt tggacagcca gcgcccggtt
     9961 ttttcaaacg tgtggtcata atcccagggg atgaagaaaa accgcgcggc aggttcatcg
    10021 ccgcgcgcga gatagaagtt ggtggtgcgc ccgtccacat tttgcgccaa attaagcaac
    10081 aacaaaaagt caatggcgtt ggccagatcc acgtgggcag cgatattatg tttaaaatcg
    10141 tccggagacg actggctggt aaaagttaaa agtccggtta gatcagcccg ccgatccgcc
    10201 cggctcgggg aggggaaaat ctgatcagcc gcttccggtt ccggttgctg gaacaaatgg
    10261 ggggaggggc gaatcttgaa aatggcgccg ccgggattgt ccgggacaac cggcgccaag
    10321 ccggtcatgt tttcgtccac ccgtgtgcac atttcataaa tgccaaaata agcgccattg
    10381 aaaaacactt cgcaccattc cagttcgggg gcgaaacgtg gttgatccgg cgtggcacag
    10441 gcgcggaaaa aatcgtaggc cagtttgttt ttcagtttgg tggcatccgc atagccattc
    10501 aaaaacaaca ggtatgggga attgccggct cccagaatgg actgcgaggt gtcgaaccgc
    10561 agcgagaacg gtttccgccg gccacgccaa taactggtgt taccacgatg tttgatgccg
    10621 ccaccggaag cctgcccgcc ttggagccat tggggttcgc ccccgccggc aggatcaaaa
    10681 cgcgccgaga attcaacacg atgagtcgcg ctcaaagctt ccgctacgga caggtggatc
    10741 gctgccagtc gcggtgtttg cgggatcacg cggaaggcga gcgtttgttt ttgccgtgtc
    10801 ttgcccgtgc ccaaggtggc cgtcagtttg acatccaccg gtacatcgcc ggctgggcgg
    10861 gtgacgtggc cttggttgtc cacgatgttc ggccgtgagc tatgccaagt cagttttttc
    10921 ccagcccaac ggacggccgc caaatccaaa tcctcgacaa tatagaatgg ggcgggattg
    10981 ttgccgagga tggtgaatga cgtcagcggc agtatctccg gcatgtcgtc tgcttcctgc
    11041 catgggcgga atcgccaagc catttcgcgc gggctccacg gattgaacag atcatgcgcc
    11101 aacagtattc gcgcttctcg cagcccggtg ctgagttgtt ctgctgtcag ggtagggaca
    11161 tccggctggt gtttcgcgcc gcgaaagagc gccgtccaat cattgggatt tcgcgcgcca
    11221 tcggctaccc atgtgcgttc cccggggcct acgccgcgtt gcgcgaaggg aattgccaca
    11281 tacgcgccct tggattggcc atcaataagc aagcggcaca acttggggcc ggcggcgtga
    11341 ttggttggtg tggtggccag cacggcttgg gcgagagtgg gcagcctatc cgccaagttt
    11401 tcgggtggca caagacgcaa cgtgcgcgca ccgaaaatcg gcgattccgg aggcagctcc
    11461 agaatgtacc ccgggcgttt ggcgatggga taactggccg ttgaaccgtc caattgcaga
    11521 tgcgcctccg cggtttgaga ggcatggagc gcgtaaatgc gccgccattt ggcgccttca
    11581 tccgtacggc ggcccgaggc caggctggcc tcgtgtgtat tgcgaaaatg ccgggcatcc
    11641 ttggaactga accgcaattc aatggttggc aaagtagcca gccgcccctc atgcaacagg
    11701 ggattgaaac gggtgagcaa gttgagcgtc gccggcaatg ccgcctgcca catggcatac
    11761 attcgccagc ggaaccaagc aaacggctcc agcgcgcgtg gcaacgaacg atgcgcgtgg
    11821 gccagccggt ggatttcacg cgacgatagg ctgcgtgtcc atgccgccgc ttcgtcaatc
    11881 gcgccgcaaa acggcgcggt aacggcatac atccgagtct tcccaaactc aatgttttgc
    11941 gtcggatgcg aaacatcggc aaagggaact gtcgccttca atgtgccgtt ttcgtacagt
    12001 tgcgcggtgc cttgcgcggc atccaccacg ccggccaaat gtacaaactt gccgtaggcc
    12061 gtgaacgggt aatgcgccgc cggcgattcc gccgaccccg gcacaaagaa catcatctgc
    12121 ccattttgca gtttgaagcc ggtgtaccgc ccttgaataa aggtgtacca gatttcctgg
    12181 tcatggcagg cgggctccac tttgacccac aacgacagcg tataattcga acccaatgcc
    12241 ggccatatcg ccgtggtttc cagcatagtc cgggccttgc cgtcaaattg ccgtgcaaaa
    12301 cccaatcggc ctggaacacg tgtcgtgccc ggatagagca ccgtgccgcg tgcgatccac
    12361 tccaccgggt gcgcttcgtc aaacgtcatc aaaaatgcca agccatggtg tttggccaac
    12421 cggtgcccaa ccccttcaaa gtagtgctgc gacttttccg ccggccactc aaataaccag
    12481 cgatccgacc gccatagggt caccgcacca gccgccatgg ccagtaggag aatccaacgc
    12541 caaaaggaac gcagagtttt cttcattgcg aaaacactcc cgtgggcagg cgcaacagaa
    12601 caaacgaatg cggcttcaca gccgtcggcg cgggcttccc atgccacagg tcgcgcgcgc
    12661 cgggccacgc gggcaatgcc gtcgcgtggt cgcctttgtt gatagcacag acaatggcag
    12721 tgtcgcccaa ctcgcgcgcg aagataacgg tgtgctcatt gggcgtgggc agccagcgga
    12781 acgaaccgcg ccgcaaggcg gactccgcgt ggcgcaacgc gatccagcgt tggtaatagg
    12841 ccaatagctc gccgtcaggc cgacgcgggg tggcctgcgg caaggggccg tggtagccgt
    12901 gggcttcgtc atcatagacg atgtcgctcc acagcatggg ttggcggcaa cagggatcgt
    12961 ttgcgcccca catgccgact tccgtaccat aataaatcat cggggcgccg gggccggcaa
    13021 actgccacgc cacagccagt tttagcgcct gtttcgcacg ggtccccggt tgacgagtga
    13081 ccaagccggg gttgttggcg gccttggccc agtggaaata ctcatcccac ccggagaacg
    13141 gcgggcatgt gctttccagc atcgtaagga cccggcctgt gtcgtgggaa tccaacagat
    13201 tttgcaatat atggacggtg ccgggttcgt gccgcgcgtg gagcgtattg atccgttgtt
    13261 gaaattccgc cgcgccgatc gcttgcggat gcggcgtgaa aaacgagagg gtggggtagg
    13321 tccattcgta gttcatcacc gccgaaaact cgtcaggtcg caaccaatct tgagccgggc
    13381 cgacaatttc ggcggtggta tagacctccg ggttgattcc gcgcgcgtag gcgtgccact
    13441 cgcgccagaa accgtgcggg acgcaatagg ccacatccag ccgccagcca tccaccccct
    13501 cggcgggcca ccccgcgccg gaagggtcca tccagcgccg tgtgatggcg aacaggtatt
    13561 gcttgatgcc ggggttcagc gtctccgcgt cacgaccaaa attgggcagc gtgccggcgc
    13621 catcccaact ggcgtaggca aacgtgccat ccgcacgcca ttcactgatt tgataccaat
    13681 ccgcgtagcg tgattttgga ccgagtcggc gcacatcctg aaatgcaaag cagccggttc
    13741 ccgagtggtt gaaaacgcca tccaaaatta ctttcatgcc gcgtgcgtgg gcttcagcca
    13801 ccaaatcaag aaaatagcgg tccgccgcag tccaaatcca ggtggcgggg tcttctgttt
    13861 cattggcggc ggccagcgcg cgtaaatcac catcacgatc gggacccagc gtgggatcaa
    13921 tgtggtgcag cgctatggca tcatatttgt gcaacgacgg cgcccagaaa atcgggttga
    13981 aatagagcgc gttaacgccg agttgctgca aataagggag ttgctcgcgc acgcccacca
    14041 aatcgccgcc gaagcgacgc tggaaaaccg tcgtccagaa gttggcggcg tgcggttgct
    14101 cccagtcgtc gcgtgcgtac caatccatgc cccatggtga aacccgccag ccggtgaatg
    14161 gcacctcgcc gatgtcctcc ggccgcggat tggattgccg cgcaccgttg cgaaaacgct
    14221 cgggaaaaat ctgataccaa acggcatcct tggcccattc gggtgtggcg ggcggatttt
    14281 gctgtgccgc aatagcggct ggtgccggag gcggaaaggt catggctcgc ggcaaagtga
    14341 cttgagccaa gccagatatt ccggcgcgcg cggatcgcct tccggtgggg caaagggcgc
    14401 tttttcaatc ggccgcaacg gtacatacgg gccggccttt gcttcaaaga acacggtgcc
    14461 atcttccagc gccacccagg cgtggtaggt gttgtgtgga acatctgcca cctgtccagc
    14521 ttgcagccgg cgggccaaca ccacttggcc ctgctcatca aaaataacca cgcccaatgt
    14581 cccgcgcaac agcaccagtg actcatcttt ggcggcatcc gcatgacggt gcggcgccac
    14641 atacgtatcc gcgcacatgg cattgaaaaa ccggtggcag ggataatcgt cgctcgcgtg
    14701 caaattgaag ttcttgcgcc gccgcggggt ggcggcagct tcggcggtca gccggtcaag
    14761 aaagtcggca tcaaacacac ggacattggg catgttcatg gcccgtcatc atagcacagg
    14821 cccccgccaa gcaaacggga aatgcggcgg ggctggggct tcactcctgc ggcggggaaa
    14881 ggcgcaacac gcggcatccg tgcggcggaa tttccgccgc aaacgattcc atctcgccaa
    14941 ggtcttcatg cgtccaaagg tcgcggactc tggccgcttc ggctaaaccc agcgccgcaa
    15001 aattgagccg gcgcgaccgg ggttcattct cgcgattgaa taacccgatc acccaatcgc
    15061 cgccggaaag ctgcccaaac cagatttggc tttcctcttg cgtggggtcc cacgacagcg
    15121 gttggcccac aaagccgtcg cggttcagtt ccagcaattc gttgttcgtg taaaaacgca
    15181 ggttgtcccc gatggtgtcg tgctgatcag ccacggcgat gggggcgccg gccagcaggc
    15241 attgggcaat cacgctgcgt ttttcatcat cggtggcaaa cgtgttcagg cgaatgaagt
    15301 cggggtccaa aatgatccgg ccgcgcccgg cgaaacgcga ccaataggcg aggccatcgg
    15361 cggcattgcc atacaccgac cagccctcgc gtttttggcc gcggaaccat tcgctccatt
    15421 tgttccagcc gccttcgccg gtgtcttcat tgacgcgcat catgtggccg tagcgccgtt
    15481 ccgcggcggc atcgtgattc aagtggggca tcacgaagct caggagcatt ccgcgctcat
    15541 cagcggcttc gcgcatccag cgcagcgcgg tctcgtaatg cgcgcggggc cgcgccgggc
    15601 ccacgcgccc caaccagcgg tcctggccgt cttcgtacca cgacaaaaaa tcaatgcgga
    15661 agaaccggat gcccatgtcg gcataaaaat gaatccagcc cttgacatac tgctccgcgc
    15721 cggggcgctc cacctgcacc cacgggaagc gggcgtcttc cgccgggtct ttgagcgaag
    15781 ccaccagaat atccgtgccg acaatccgcc gctccgtgtc atcatcgttc acgtgcaccc
    15841 agagcggatt gccatacatg cccatggcga ggccgcgctc gcgcaagtag gccgcccacc
    15901 acgcgtaatc atgctcccaa tggcgggagt gggtcaggcg gtagccgttc tgattcagcc
    15961 gggaaacatc gccccagcca tccacgcaca ccatatcata gccgaagggt ttgaggttgg
    16021 cctccaccca gttgatgttg gccagccact ccgcttccgt gatgtacggc tcaggctggc
    16081 cggcctgttc cttgcggaac aggttttcgt acacgctcca ataaagcggg gcgtgaaacg
    16141 ggttggtttg ggccggggcg ggcgtggcgc acaacagcgc ggccagcagc gcgcccgccc
    16201 attttccaca g
//
LOCUS       DAOPNP010000052        15798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4422_length_15798_cov_9.014546, whole genome shotgun sequence.
ACCESSION   DAOPNP010000052 DAOPNP010000000
VERSION     DAOPNP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4422_length_15798_cov_9.014546"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1242)
                     /gene="ilvC"
                     /locus_tag="PKI64_06180"
     CDS             complement(142..1242)
                     /gene="ilvC"
                     /locus_tag="PKI64_06180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOE00635.1"
                     /translation="MAKINFGGVKENVVTRAEFPLKKAQQVLKNEVIAVIGYGVQGPA
                     QSLNMRDNGIKVIIGQDPRFANDWQRAVQDGFQPGKTLFDVEEACAKATIIMMLVNDG
                     ALKYVWPRVQPHLTKGKALYFSHGFGWVYRKLTGVKAPKDVDVILVAPKGSGTSVRRN
                     FLAGVGINSSFAVAQDATKRARQRTLAIGIAVGSGYLFPTTFEHEVTSDLVGERGVLM
                     GALAGIMEAQYDVLRQNGHSPSEAFNETVEELTQSLIRLVDENGMDWMYSNCSATAQR
                     GALDWKPRFKAAVMPVFKKLYTAVKSQAEAKRVIKVCGAPDYKKKLDAELKAMGTSEM
                     WRAGAAVRALRPHEKAKANAATAAGVRGRGKN"
     gene            complement(1366..2283)
                     /locus_tag="PKI64_06185"
     CDS             complement(1366..2283)
                     /locus_tag="PKI64_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00636.1"
                     /translation="MTAAIQAEHLAKTFRDFWQRPRVRAVQDVTFEVPAGQVFGLLGP
                     NGSGKSTIIKMILGLLFPTAGRLTVLGASPRATRVKERIGYLPEESYLYPHLTPQETL
                     AFYARLFRLPGAVARERIGQLLDMIGLTHARHRPVGEFSKGMARRIGLAQALLNDPDL
                     IILDEPTAGLDPQGTRQVKDLILALAARGKTVLLTSHLLADVEDVCGQVAILFNGRIL
                     AQGTLDSLLARQDQTTLTFPTPAPATLAQVLAAARETLGAVPELAHPRQALEPFFLDV
                     VRRAAGTDSAAAAPTGATPAAPLAPFLRG"
     gene            complement(3318..4280)
                     /locus_tag="PKI64_06190"
     CDS             complement(3318..4280)
                     /locus_tag="PKI64_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00637.1"
                     /translation="MAALLQFAAMARLAALEAIRRPVFLLVALVSLGGTVLLPFLLNY
                     TLGDSARIIRDSALALYLTSGLLLAAYAADEGLGRELRRGTAATVLAKPVARPVFFLA
                     KAAGIIGALALYSIAALAATLLATRAGASDIRLDWAAQTPALLALLLAPALAGWWNHR
                     TRRPFTSAAFFLLLIFLLAALAIAAGFPTPLDHLTFPANFDWPILAVGALLFCCLAMV
                     AAAALTLATRLSVTPVLLLTAGLFVFGLMSDYLLGPRLATSLAARVAYAILPNVQAFW
                     LLDALDAATPIPAAYFLGAAAYAAAWSAAALAIGIALFQRQEIT"
     gene            complement(4286..5119)
                     /locus_tag="PKI64_06195"
     CDS             complement(4286..5119)
                     /locus_tag="PKI64_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00638.1"
                     /translation="MNRLPPLLIALALSGAAAFAQQRIEFQRQKFDRSRFERPGTPGA
                     PAEPALPPAEPVGTTPSSASTPMLTVPPESSSIFSYSNEPSTGGSTWSSGASTNTGGG
                     SIFSADAPALSGPPPYAYPANSTPEERAAIDSPHFDQVPSKWFTGADRYPQLLALQKE
                     TGACILVYFKRVSEASEKGLCSWFEKGFYPDIAWRKAMRYFIKLEIQLPGGSATRELA
                     EQFKVNKTPAIFVVKPGSTFHQRLMVFDYPKGSRPEPIPVPTVLNNLKERATPAYQNL
                     F"
     gene            complement(5141..6157)
                     /gene="corA"
                     /locus_tag="PKI64_06200"
     CDS             complement(5141..6157)
                     /gene="corA"
                     /locus_tag="PKI64_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOE00639.1"
                     /translation="MLRIILDRIAAMIRTFVFSQGKLLNQDLTLDLLRVVLYDDDVQI
                     WVDLEQSTPEENKAVLETVFDFHPLAIEDCLAISERPKIDDYDNYIFVVMHAVNHIAS
                     SDDFQTTEVNFFIGRNFLVTFHDEPVRSISHMVERIQKKGHAVPRAPDLLAYQILDLL
                     FDNYQPALDELSAELTDLEHRVLTGNSKDVLNEVVNLQGQVQRLRQLTTPQREVIGRM
                     MRGEFSKVIRSHLTPYLRDLSDNLSRINDLAETFRESLTSTLQVHLNLQQMSVNRVIK
                     VLTILATLSMPILLVTSFYGMNIQHFPNTEWPSWPWAYLFTFVLNGVLVTLLYRLMKR
                     HNWL"
     gene            6265..6855
                     /locus_tag="PKI64_06205"
     CDS             6265..6855
                     /locus_tag="PKI64_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE00640.1"
                     /translation="MAFTLELGAPAPDFQLPATDGRTYALADFAEAPVLVVFFTCNHC
                     PYVIASDETTRATVEKYQPRGVRFVGINSNSRHTYAEDDFPHMVRRMAEHRFPWVYLR
                     DETQQVALAYGALRTPHFYVFDAARRLVYTGRGLDNPREPAKATINDLENALDDVLAG
                     RPVRVPQTNPIGCNVKWEGRDKHWMPPDACDLVPPR"
     gene            complement(6852..8372)
                     /locus_tag="PKI64_06210"
     CDS             complement(6852..8372)
                     /locus_tag="PKI64_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE00641.1"
                     /translation="MTTRRGRPLLELRHAAFRLGPRLVLTDSNWTLRRGEHWAIVGGT
                     GAGKTLLCRALCGDLPLVRGTLDYHFPAPPRREPETAIAYVSAEQWSHDPVEGAPIRW
                     FSLDRELSPLVRDQLSWRNVENINPFEVVRRRAADSARWQRRARRVIRWLDIEPLLPK
                     PMLALSNGERRKVALARALLRAPRILILDDPFAGLDTRYRRHLRQLLARLITQRATTL
                     LLVGAQPADWPRGLTHLLHMDRLRIAAQGPLAPLKRALRAAGALPAAMPTVPRHAAQS
                     AALGTELIRLTDVAVRWNDTPLLEHVNWTVRAGESWALLGPNGSGKSTLLSYIIGDNP
                     QVYANDVRVFGHARGSGESLWDIQRHIGWVSPELHAGFDGTLTGLATVMSGFFAATPF
                     ARKFTPRQHAAARDALRRLRLMRLADAPFAAQSTGEQRLLLLARALVTKPSLLILDEP
                     CQGLDHAHRARLLAIVDQQIRRGTTVLFVTHRRDEIPPAIRRVLRLRHGRAVAGVL"
     gene            complement(8369..9139)
                     /locus_tag="PKI64_06215"
     CDS             complement(8369..9139)
                     /locus_tag="PKI64_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE00642.1"
                     /translation="MRITFLGTGTSYGVPMPGCTCAVCQSPDPRDKRFRTAILIEASG
                     QTILMDTPPDLRSQCLAHNIQKIDATLITHAHADHVFGFDDLRPYTNRMAEPMPVWAS
                     PGTVQALRRVFDYLDRPPIPGTSQARIALHTAEAPFDFHGIRCTPLPATHGYADMIGW
                     KLEHAGRSFAAIPDCKELPAETLALCRGVDLVVVDALRVRPHPTHMNFAEAVAVLDQI
                     QARQSLVIHLCHEVSHEQARQLLPPHIAPSYDGLQIEL"
     gene            complement(9216..9839)
                     /locus_tag="PKI64_06220"
     CDS             complement(9216..9839)
                     /locus_tag="PKI64_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOE00643.1"
                     /translation="MKRFMRNGCATGLVLLALLVVSVLTARLGLYPFLARQAPVATDT
                     LVIEGWLSDPVLAQLDSWLATNAIKRIYTTGGPLETGSYLIAWTNYADLTRARLIQLG
                     YDQRYELTAVPAPKVRRDRTRESARALRAVWPLERGAFNLVSEGPHTRRSWRAFQQVF
                     GDGVEVGSIALVPEAYDARDWWTCSEGLRTVLTETFGYIYDRLFGAP"
     gene            complement(9836..10048)
                     /locus_tag="PKI64_06225"
     CDS             complement(9836..10048)
                     /locus_tag="PKI64_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00644.1"
                     /translation="MKTWRIARKHVLSIKFACPECGQHIDAGRELFGEMVECPTCGHL
                     MQAPDLKIGPGDLKPKRLPRPNPHRP"
     gene            10184..10873
                     /locus_tag="PKI64_06230"
     CDS             10184..10873
                     /locus_tag="PKI64_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HOE00645.1"
                     /translation="MKVERLSAKAEDYLETILRLSETPRGARTGDIAAELNVQPSTVS
                     AALRSLMEQGLVDYTPYGAVGLTPKGRQVAEEISGRHVALRRFLTETLGLPPEDAEVA
                     ACGMEHDATPLVVERMGLLGDFLRDHPGMLAKWLALRETAKGERAPGVESGLDELPPG
                     VRGVVMAVDVEGSLRKRLVEMGVTPGVLIAVERVAPLGDPIAVEVRGYRLSMRKAEAA
                     RIRVKWRPGLV"
     gene            10881..>12976
                     /gene="feoB"
                     /locus_tag="PKI64_06235"
     CDS             10881..>12976
                     /gene="feoB"
                     /locus_tag="PKI64_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOE00646.1"
                     /translation="MAAKATTAAPELVILLAGNPNSGKTSLFNWMAGAHQHVGNYPGV
                     TVEKKSARFTRDGIKIELVDLPGVYSLLAASPEEVIARDEILSGTAGVVIQVLDATNL
                     ERNLHLTAQLTEMGVPVVYALNMSDEAAANGLVFDLPLMGQLLGGPVVPTNGSTGAGT
                     DALLAAAQLLAATGQPGASRLHYGLDIEQSIKDIAEHFEGDDAVSARRRAVALLDGMA
                     DPHAPAAATTVAAREAVRLTKRGGRPLDVQLAEARYGFALGVATEVVRATGHHVRRDR
                     TMRIDSVLLHRVWGLPIFVALMWVVFNLVFTLGEPPMQWIESGLGWLAAHLQAVWPDT
                     IAPLLKSLLVDGVIGGVGAVISFLPNILLLFLAIAFLEDTGYMARVAFLMDRIMHKMG
                     LHGKSFIPMLIGLGCTVPAIMATRTLEHKRDRFTTILVSPFVSCGARMTIYALLIPAF
                     FAPRWRGWVLWGIYMTGIVAAIGIARLLRGTLFRGETAPFVMELPPYRLPTWRSVGIH
                     MWDRAWMYLQKAGTIILAISVLLWALGTFPRRVDYSRDYAAAMAQAATPEAAADLAAQ
                     QQAETLEYTIIGRLGKVLEPVIRPLGFDWRVGTALVGAFAAKEVFVAQMGIVFSLGEA
                     GGNPDDLRATLRQNYTPLQGLAMMLFCLLSAPCMATIAVTRRETDSWGWALGMLFGMT
                     MVAWICTFVVYRLGLL"
     assembly_gap    12977..13076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13160..14683
                     /gene="lnt"
                     /locus_tag="PKI64_06240"
     CDS             13160..14683
                     /gene="lnt"
                     /locus_tag="PKI64_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOE00647.1"
                     /translation="MKITPATRLEWLAALGAALTAGLLQAVLAPLEWTACAWVALVPL
                     LIVARLVPGRLALKMGFVTGGLFWLISIRWLTQVTVLGWVALSAYCALYFLPPVLVAN
                     RWRGGGGSFAIAVAVAWSASEFIRGWLGTGFPWNPLGAGLAPWLPGIQLAEIGGVWLV
                     SGLVVAVNALLAWALVEQRHWRSLALGALLVAAALGWGQWRVHHLPTPARHIRVALVQ
                     TAIPQDEKWVAAKIRMIYDRLITLTRQAQGDPDVELVIWPETALPDDVRNSEASYTVV
                     WSLCTNGTSILTGTLDTAVLDDGTEQYFNSVFLFDTEGRIVGEYDKRHLVLGGEVIPL
                     ERWIPDRWMLALGLPTSITAGQKGAVFTAGRQQVPLAPLVCFEDILPHLARADVRAGA
                     RALVNVTNDAWFDEVIAPRQHMRNAVLRAVENRVPLIRAANTGVSCVIEPSGAVAAML
                     SDGAGGTAGAGVLWADVAVPDPTAPLTCYARFGDMYAFACLAATGLWLLAAWRESRT"
     gene            14694..>15798
                     /gene="prfB"
                     /locus_tag="PKI64_06245"
     CDS             join(14694..14759,14761..>15798)
                     /gene="prfB"
                     /locus_tag="PKI64_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537001.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOE00648.1"
                     /translation="MLDELSTRIATVKENLAGMRGYFDVAGKKRLIEELTQQMSAPDF
                     WDDQNAARALIEKKNTLEAVTEPYDKLVALQEELEVMAALLGEAGTDSPTHPDVVELA
                     KQVAALEKQYAHVELQSLMVGRFDANNAFLTLHAGAGGTESCDWAAMLLRMYDRYCQQ
                     QGFEVTTVDYQPGDEAGVKSVTLMVSGPWAYGFLKAERGVHRLVRISPFDANKRRHTS
                     FASLDVVAEISDDIEVTIEDKDLRVDTYRSSGAGGQHVNKTDSAIRLTHLPTGIVVAC
                     QAERSQLANRNKAMKILRAKIYELEQDKKRREMEKFYGEKGEIAWGHQIRSYVLQPYT
                     MAKDHRTDVETSQVFEVLDGDLGLFIEAWLKQNR"
BASE COUNT         2813 a         4491 c         5582 g         2812 t
ORIGIN      
        1 acatcaccat gatgcatgaa cgaaacccca tagcatctac aatccaaccc ggatgcggga
       61 cgctggaaat tgccccaagc aagtgcgcac aaaaaagccc gccgactggg ggtcggcggg
      121 cgtgatccac gcagggcaga tttagttttt gccgcggccg cggacaccgg cggcggtggc
      181 ggcattggct ttggccttct cgtgcgggcg cagggcgcgc accgcggcgc cggcgcgcca
      241 catttcggag gtgcccatcg ccttgagttc ggcatcgagt ttcttcttgt agtccggcgc
      301 gccgcaaacc ttgatgacgc gcttggcttc ggcctggctc ttgacggcgg tgtagagctt
      361 cttgaacacc ggcatgacgg cggccttgaa gcggggcttc caatcgagcg cgccgcgttg
      421 cgcggtggcg gagcaattgg aatacatcca gtccatgcca ttttcatcca cgaggcggat
      481 caggctctgg gtgagctcct cgacggtctc gttgaaggcc tcggaggggg agtggccgtt
      541 ctggcgcaac acgtcgtact gcgcttccat gatgccggcc agcgcgccca tcagcacgcc
      601 gcgttcgccg acgaggtcgg aggtgacttc atgctcaaac gtggtgggga acagatagcc
      661 cgagcccacg gcgattccga tggccagcgt gcgctgccgc gcgcgcttgg tggcatcctg
      721 cgccacggcg aagctggaat tgatgcccac gccagcgagg aagttgcggc gcacggaagt
      781 gccggagccc ttgggggcca cgaggatcac gtccacatcc ttgggcgctt tcacgccggt
      841 cagcttgcgg tacacccagc cgaacccgtg cgagaagtac agcgccttgc ccttggtcag
      901 atgcggctgg acgcgcggcc acacatattt gagcgcgccg tcgttgacca gcatcatgat
      961 gatggtggcc ttggcgcagg cttcttccac atcaaagagg gtcttgccgg gctggaagcc
     1021 atcctgcacc gcgcgttgcc aatcgttggc gaaccgcggg tcctggccaa tgatgacctt
     1081 gatgccgttg tcgcgcatgt tcagcgactg cgccgggccc tgcacgccgt agccgatgac
     1141 ggcgataact tcattcttca ggacctgctg ggctttcttg agggggaact ccgcgcgggt
     1201 cacgacgttt tccttgacgc cgccaaaatt aatcttggcc atgttgctac tccttctttc
     1261 gttatgaact ggaaaccaaa caggggtgaa caataggcgc agggcggggc cgaggcaagc
     1321 gggttttgca aaaaaatggg gcgagcggcg tggcgggccg gcggtctagc cgcgcaaaaa
     1381 cggcgcgagc ggcgcggcgg gtgtggcgcc ggtgggcgcg gcggcggcgg aatccgtgcc
     1441 ggcggcgcgg cggaccacgt cgaggaaaaa cggctccagc gcctgccgcg ggtgggccag
     1501 ctcgggcacg gcgcccagcg tctcgcgcgc ggcggccagc acttgggcca gcgtggcggg
     1561 ggcgggggtg gggaaggtga gcgtggtttg gtcctgccgc gccagcaggc tatccagcgt
     1621 gccctgcgcc agaatgcggc cgttgaacag gatggccacc tgcccgcaca cgtcctccac
     1681 atccgccagc aggtgcgaag tcagcagcac ggttttgccg cgcgcggcga gggcgaggat
     1741 caaatccttg acctgccggg tgccttgcgg gtcgaggccc gcggtgggtt catcgaggat
     1801 gatcagatcg ggatcgttca gcagcgcctg cgcgaggccg atgcgccgcg ccatgccttt
     1861 ggaaaactcg ccgacgggcc ggtgacgggc gtgggtcagg ccgatcatat cgagcagttg
     1921 gccgatgcgt tcgcgggcga ctgcgccggg caggcggaat aagcgcgcgt agaacgccag
     1981 cgtttcctgc ggcgtgaggt gcgggtacag atacgactct tccggcaggt agccgattcg
     2041 ttccttgacg cgcgtggcgc gcggcgatgc gcccagcacc gtcagccgcc ctgccgtggg
     2101 gaacagcagg cccagaatca ttttgatgat ggtggatttg ccggaaccgt tggggcccag
     2161 caggccgaat acctggccgg cggggacctc gaatgtgacg tcctgcactg cgcgtacgcg
     2221 cggccgttgc caaaagtcgc ggaaggtttt ggcgaggtgt tcggcttgga tcgcagcggt
     2281 catggttcca tcctaatcac ggccggcgtt tcggcaacag ccaaactttt ccacgacggg
     2341 gaaaagaggg gtcagggttc ggggttcagg gttcaggggt tggcgggcgt gttgtgtgtg
     2401 gcgggcgtgt ggcgtgtggc gggtgtgtgg cgtgtggcgg gtgtgtgagg tggaggattt
     2461 cttgaggatt ttggcggcgc caaaaccaac cggcgtcttt tccgcgctgg cgcgccgcga
     2521 ccgacccctc gcggacacga gtccagctcg ggtcgtccgc aacgcgccat catcggaaaa
     2581 tccgccgctt attttggcgc gagaaccccg ccaaaatcca caagaaatcc tccgcgcgca
     2641 gtgctgcgca cagcggacaa ggcccggcag ggccttatgg ttaccgctcc gcctcgcgca
     2701 acgctcggcg gagagatcag gggtgcggat tcttttgggg acggcttcca cgccgtctgg
     2761 gcagttggtg cggggggcgg acctgaactc tgaaccctga accctgaccc ctccgccgcc
     2821 ttgcggcggt gcgccatcat cggaaaatcc gccgcttatt ttggcgcgag aaccccgcca
     2881 aaatccgcaa gaaatcctcc gctcgcagtg ctgcgcacag cggacattgt tgcggcgttc
     2941 ggattttcca cagtgtggaa aaatattttc cacactgtgg aaaaagtggg caaaaaagtt
     3001 ccacgctgtg gaaaagttgg gcccgttttt tccacacaat ggaaaaagtt tcagagattt
     3061 ttccacacaa tggaacaact ttgcgggaat ttttccacac tgtggaaaaa tggggccaaa
     3121 aagttccacg caatggaaaa acttttggcg atttttccac gcaatggaaa ataggcgagt
     3181 gccgagcccg agcccgaagg gcgatgggcg aacgcgagct ctatagcgac cggtgcagcc
     3241 gggcgagcca gcgagccata gcgagcgcag cggcaaactt tggggcggat tttccacact
     3301 gtggaaaagg ggggtcatca ggtgatttcc tgtcgttgaa agagggcgat gccgatggca
     3361 agggcggcgg cggaccacgc ggcggcgtag gcggcggcgc cgaggaagta ggcggcgggg
     3421 atgggggtgg cggcgtcgag ggcatcgagg agccagaagg cctgcacgtt gggcaggatg
     3481 gcgtaggcga cgcgcgcggc aagcgaagtg gcgaggcgtg gcccgaggag atagtcgctc
     3541 atcaggccga acacaaagag gcccgcggtg agcaggagca cgggggtgac actcaaacgc
     3601 gtggcgagag tgagcgcagc ggcggcgacc atggcgaggc agcaaaagag cagcgcgccg
     3661 acggcgagga tgggccaatc gaagttggcg gggaacgtca ggtggtccag cggcgtggga
     3721 aagccggcgg cgatggcgag ggcggcgagc aggaaaatca gcagtaggaa aaacgcggcg
     3781 gaggtgaagg ggcggcgcgt gcggtggttc caccagccgg cgagggccgg cgccaacagc
     3841 agcgcaagga gcgcgggagt ttgtgcggcc cagtcgaggc ggatatcgct ggcgccggcg
     3901 cgggtggcca gcagggtggc ggcgagcgcg gcgatgctgt agagcgcgag cgcaccgatg
     3961 atgccggcgg ctttggcgag gaaaaacact ggccgcgcga cgggcttggc caacacggtg
     4021 gcggcggtgc cgcggcgcaa ctcgcgcccg aggccttcgt cggcggcata ggcggccagc
     4081 agcagtccgc tggtgaggta gagcgccagt gcgctatcgc ggatgatgcg cgcggagtcg
     4141 ccgagcgtgt agttcagcaa aaagggcagc aggacggtgc cgcccaaact caccagcgcc
     4201 accagcagga aaaccgggcg acggatggct tccagcgcgg ccagccgggc catggcggca
     4261 aactggagca gcgcggccat gcgcctcaaa acaggttctg gtaggcgggc gtggcgcgtt
     4321 ccttgaggtt gttgagaacc gtgggcacgg ggatgggttc cgggcggctg cctttgggat
     4381 agtcgaacac catcaggcgc tggtggaacg tgctgccggg tttcaccaca aaaatggcgg
     4441 gggttttgtt gaccttgaat tgctcggcca gctcgcgcgt ggcggagccg ccggggagtt
     4501 gaatttccag cttgataaag tagcgcatgg ccttgcgcca ggcgatgtcg gggtagaagc
     4561 ctttttcaaa ccaactgcag agccctttct cggaggcttc cgagacgcgc ttgaaataaa
     4621 ccagaatgca tgcgccggtt tccttctgga gcgcgagcag ttggggatag cggtcggcgc
     4681 cggtgaacca tttggagggg acttggtcga agtgtggcga gtcaatggcg gcgcgttctt
     4741 cgggcgtgct gttggcggga taggcgtaag ggggggggcc ggagagcgcg ggagcgtcgg
     4801 cggaaaaaat ggaaccaccg ccggtgtttg tgctggcgcc ggaagaccac gtgctgccgc
     4861 cggtgctggg ttcgttggag taggagaaaa tggaggacga ttccggcggc acggtcagca
     4921 tgggcgtgga agcgctggag ggcgtggtgc cgaccggttc ggcaggcggc agcgcgggct
     4981 cggcgggggc gccgggggtg ccggggcgct cgaaacggct gcggtcgaat ttttgccgtt
     5041 ggaattcaat gcgctgctgg gcaaatgcgg cggcgccact cagggccaac gcgatgagca
     5101 gggggggcag tcggttcatg ggcggtactc cggttgaggg ttacaaccaa ttatgacgtt
     5161 tcatcaagcg atagagcagg gtcaccagca cgccgttgag cacgaacgtg aacaggtagg
     5221 cccacggcca cgagggccat tccgtgttcg gaaagtgctg gatgttcatg ccgtagaaac
     5281 tggtgacgag caggatcggc atcgagagcg tggccagaat cgtcagcacc ttgatgacgc
     5341 ggttgacgga catctgctgc aggttgaggt gcacttgcag cgtgctggtg agcgattcgc
     5401 ggaaggtttc ggcaaggtcg ttgatgcggg aaagattgtc cgaaaggtcg cgcaggtagg
     5461 gggtgaggtg gctgcggatg accttggaaa attcgccgcg catcatgcgg ccgatgactt
     5521 cgcgctgggg ggtggtcagt tggcgcaggc gctgcacctg gccttgcagg ttgacgactt
     5581 cgttgaggac atccttggag ttgccggtta gcacgcggtg ttccaggtcg gtgagctcgg
     5641 cggaaagttc gtcgagcgcg ggctggtagt tgtcgaagag caggtccaaa atctggtagg
     5701 ccagcaggtc gggggcgcgg ggtacggcgt ggcccttttt ctggatgcgc tcgaccatgt
     5761 ggctgatgct gcgcacgggc tcgtcgtgga acgtcaccaa aaaattacgg ccaatgaaga
     5821 agttgacctc ggtggtctgg aagtcgtccg agctggcgat gtggttgacg gcgtgcatga
     5881 ccacgaaaat atagttgtcg tagtcgtcaa ttttggggcg ttcgctgatg gcgaggcagt
     5941 cttcgatggc cagcgggtgg aagtcgaaaa cggtttcgag cacggccttg ttttcttcgg
     6001 gcgtggattg ctcgaggtcc acccagattt ggacgtcatc gtcgtagagc accacgcgca
     6061 ggagatccag cgtgaggtcc tggttgagca acttgccttg actgaaaaca aacgtgcgaa
     6121 tcatggccgc aatccggtcc aaaatgatgc gcaacatgat gccctgcggc gggggcactg
     6181 gcaaggaaaa gcggcgcgcg cggtttgcca agccggcgcg cgtggggtag gctgaaaacc
     6241 agcagaaaac cgaggagaat gaccatggct tttacccttg aacttggcgc gcccgcgccg
     6301 gatttccagt tgccggccac cgacggccgc acctacgcgc tggccgactt tgccgaggcg
     6361 ccggtgctgg tggtgttttt tacctgcaac cactgtccct acgtgattgc ctccgacgaa
     6421 accacgcggg caaccgtgga aaaatatcag ccgcgcggcg tgcggtttgt gggcatcaat
     6481 tccaacagcc gccacaccta tgccgaggac gactttcccc acatggtgcg ccggatggcg
     6541 gagcatcgtt tcccgtgggt gtatttgcgc gacgaaacgc agcaggtggc gctggcttac
     6601 ggcgcgctgc gcaccccgca tttttatgtg ttcgatgccg cgcggcggct ggtttatacc
     6661 gggcgcgggc tggacaaccc gcgcgagccg gccaaggcca cgatcaacga tctggaaaac
     6721 gcgctcgacg acgtgctggc cgggcggccg gtgcgcgtac cgcaaaccaa tcccatcggc
     6781 tgcaacgtca aatgggaagg gcgcgacaaa cattggatgc cgcccgacgc gtgtgatctg
     6841 gtgccgccgc gttaaagtac tccagccacg gcgcgaccgt ggcgcaagcg cagcacgcgg
     6901 cggatggcag gcgggatttc atcgcgccgg tgcgtgacga acagcaccgt ggtgccgcgg
     6961 cggatttgct ggtccacaat ggccagcagg cgggcgcggt gggcatggtc cagtccttgg
     7021 cacggctcat ccaggatcag gaggctcggc ttggtcacca gtgcccgggc cagcaatagc
     7081 agacgctgct cgccggtgga ttgcgcagcg aagggggcat ctgcgaggcg catcaggcgc
     7141 aggcggcgca aggcgtcgcg cgccgcggcg tgttgccgcg gcgtgaattt tctggcaaag
     7201 ggagtcgccg caaaaaagcc gctcatgacg gtggcgaggc cggtgagggt gccatcgaaa
     7261 ccggcatgca attcgggtga cacccaacca atgtgccgct gaatatccca gagactttcg
     7321 ccgctgccgc gcgcgtggcc gaagacgcgg acatcgttgg cgtacacttg gggattgtcg
     7381 ccgatgatgt agctcaacag cgtggatttg ccggaaccat ttgggccgag cagcgcccag
     7441 ctttcgccgg cgcgcactgt ccagttgacg tgttccaaga gtggagtgtc gttccagcgc
     7501 acggcaacat cggtgagacg gatcaactcc gtgccgagcg cggcggattg cgccgcgtgg
     7561 cgcggcacag tgggcatggc tgcggggagc gccccggcgg cgcgcagcgc gcgcttcagc
     7621 ggcgccagcg gtccctgtgc ggcaatacgc agacgatcca tgtgcagcag atgcgtcaac
     7681 ccgcgcggcc agtccgccgg ctgcgcgccc accaacagca gcgtggtagc gcgctgcgta
     7741 atcagccgcg ccaacaactg gcgcaggtgg cggcggtagc gggtgtccag ccccgcgaag
     7801 ggatcatcca aaatcaggat gcgcggcgcg cgcagcagtg cgcgggcgag ggccactttg
     7861 cggcgctcgc cgttggagag cgccagcatc ggcttgggca gcagcggttc gatgtcgagc
     7921 caacggatga cgcgccgggc gcggcgttgc cagcgggcgc tatccgccgc gcgtcgccgc
     7981 acgacctcaa acgggttgat gttttccacg ttgcgccaag aaagttggtc gcgcaccagc
     8041 gggctgagtt cgcgatccaa gctgaaccag cggatgggcg cgccttcaac gggatcgtgg
     8101 ctccattgtt ccgcggaaac gtaggcgatg gcggtttccg gctcgcggcg ggggggcgcg
     8161 ggaaaatggt agtcgagcgt gccgcgcacg agcggcaggt cgccgcaaag tgcgcggcac
     8221 agaagcgttt tgccggcgcc ggtgccgccg acgatggccc aatgttcgcc gcggcgcagc
     8281 gtccagttgc tgtcggtcag cacgagacgc ggacccaacc ggaacgccgc gtgacggagt
     8341 tcaagcagtg ggcggccgcg gcgcgtggtc ataactcgat ttgcaggccg tcgtaagacg
     8401 gagcgatgtg cggcggcagc agttgccgtg cctgttcgtg ggagacttcg tgacagaggt
     8461 ggatcaccag cgactgccgc gcctgaatct ggtcgagcac cgcaacggct tccgcaaagt
     8521 tcatgtgggt gggatggggg cggacgcgca gcgcatccac caccaccaaa tccacgccac
     8581 ggcaaagggc cagcgtttcc gccggcagct ccttgcaatc ggggatggcg gcgaaagagc
     8641 ggccggcgtg ttcgagtttc cagccgatca tgtcggcgta gccgtgggtg gcgggcagtg
     8701 gcgtgcagcg gatgccgtga aaatcaaatg gcgcttcggc ggtgtgcagc gcgatgcgcg
     8761 cctgcgaggt gccgggaatg ggcgggcggt cgaggtaatc gaacacgcgg cgcagggctt
     8821 ggaccgtgcc cggcgaggcc cagacgggca tgggctcggc catgcggttg gtgtaagggc
     8881 gcaggtcgtc gaaaccgaac acgtggtcgg cgtgggcgtg ggtgatcagg gtggcatcaa
     8941 ttttttggat gttgtgggca aggcattggc tgcgcaggtc gggcggcgta tccatcagga
     9001 tggtttggcc ggaagcttcg atgaggatgg cggtgcggaa acgtttgtcg cgcgggtcag
     9061 gcgattggca gaccgcgcag gtgcagccgg gcatggggac gccgtaggag gtgccggtgc
     9121 ctagaaatgt gatgcgcata aaacggaggc ggttgggttc tcaaaaatag cagcgggttt
     9181 atgctgcgcg acgcacagcc ttggagggcg ccgtttcatg gcgcgccaaa gaggcggtca
     9241 tagatgtagc caaaggtttc agtcaggacg gtacgcaggc cctcggagca ggtccaccaa
     9301 tcgcgggcat cataggcctc gggcacgagg gcgatgctgc cgacttccac gccatcgcca
     9361 aaaacctgct gaaaggcgcg ccaactgcgg cgggtgtggg ggccttcgct gacgagattg
     9421 aacgcgccgc gttccagcgg ccagacggcg cgcagggcgc gggcggattc gcgcgtgcgg
     9481 tcgcggcgga ctttgggcgc gggaacggcg gtcagctcgt agcgctggtc gtagccgagc
     9541 tggatcaggc gcgcgcgggt caggtccgca tagttggtcc aggcgatgag gtagctgccg
     9601 gtttccaacg gcccgccggt ggtatagatg cgtttgatgg cgttggtggc gagccacgag
     9661 tcaagctgcg caaggacggg gtcggagagc cagccttcga ttacgagagt gtcggtggcc
     9721 acgggtgctt gacgggccaa aaaggggtag agccccaaac gggcggtgag gacgctgacg
     9781 accagcagcg ccagcagcac aaggccggtg gcgcagccgt tgcgcatgaa tcgcttcatg
     9841 gccggtgcgg gttcgggcgg ggcaggcgtt tgggtttcag gtcgccggga ccgattttca
     9901 ggtctggcgc ctgcatcaga tggccgcagg tggggcactc gaccatttcg ccaaacagct
     9961 cgcgaccggc atcaatgtgc tgtccgcatt cggggcaggc aaacttgatg ctcaacacgt
    10021 gcttgcgggc aattctccac gttttcatgc aaccatactg cgaggaaccc acgccgatgg
    10081 caagcctcaa cgcgccgtcg ggggcgcacg gcttgccgct tgcgcggctt ggtgaatgtg
    10141 gtagtgttgg atacttcaaa catttcgaga tcacccgaaa ccgatgaaag tggaacgact
    10201 tagcgccaag gcggaagact atttggagac catcctgagg ctctccgaaa cgccgcgcgg
    10261 tgcgcgcacg ggcgacattg ctgccgagct gaatgtgcag ccgtccacgg tgtcggcagc
    10321 gctgcggtcg ctgatggagc aggggttggt ggattatacg ccctacggcg cggtgggcct
    10381 gacccccaag ggccgccagg tggcggaaga aatttccggg cgccatgtag ccttgcgtcg
    10441 tttcttgacc gaaaccctcg gcctgccgcc ggaagatgct gaagtggcgg cgtgcggcat
    10501 ggagcacgat gccaccccgt tggtggtgga acgcatgggc ttgctcggcg attttttgcg
    10561 cgaccacccg gggatgctgg ccaagtggct cgccctgcgc gagactgcca agggcgagcg
    10621 cgcccccggc gtggagtcgg ggctcgatga gctgccgccg ggcgtgcgtg gcgtggtgat
    10681 ggcggtggat gtggaaggtt cgttgcgcaa acggctggtg gaaatgggcg tgacccccgg
    10741 cgtgctgatt gccgtggaac gcgtggcgcc gctgggcgat cccattgcgg tggaagtgcg
    10801 cggctaccgg ctctccatgc gcaaggccga agccgcgcgc atccgtgtga agtggcggcc
    10861 cgggctggtg taggggacgc atggcggcca aggcgacaac tgctgccccg gagctggtga
    10921 tcctgctggc aggcaacccc aacagcggca agaccagctt gttcaactgg atggccggcg
    10981 cgcatcagca cgtgggcaat tatcccggcg tgaccgtcga gaaaaaatcc gcccgcttca
    11041 cgcgcgacgg cataaaaatc gagctggtgg acttgccggg ggtgtatagc ctgctggcgg
    11101 catcgccgga agaagtcatc gcgcgcgacg aaattttgtc cggcaccgca ggcgtggtga
    11161 ttcaagtgct ggatgccacc aaccttgagc gcaatctgca cctgaccgcg cagctcacgg
    11221 agatgggcgt acccgtggtc tatgcgctca acatgagcga cgaagccgcc gccaacggct
    11281 tagtgtttga tttgccgctg atgggccagt tgcttggcgg ccccgtggtg cccaccaacg
    11341 gcagcaccgg cgccggcacc gacgcgttgc ttgccgccgc gcagttgctg gcggccaccg
    11401 gccaaccggg cgccagccgc cttcattacg gcttggatat cgagcagtcc atcaaggaca
    11461 tcgccgagca ttttgaaggc gatgatgcgg tatccgcgcg gcggcgggcc gtggcgctgt
    11521 tggatggcat ggccgatccg cacgcgccgg cggcagccac caccgtggcg gcccgcgaag
    11581 ccgtgcgcct gaccaaacgc ggcggacggc cgctggatgt gcaattggcc gaggcccgct
    11641 atggctttgc cctcggcgtg gcgaccgaag tcgtccgcgc caccggccac catgtgcgcc
    11701 gcgaccgcac catgcggatt gattccgtgc tgctccaccg cgtgtggggc ttgccgattt
    11761 ttgtcgcctt gatgtgggtg gtgttcaatc tggtgttcac cttgggcgag ccacccatgc
    11821 agtggatcga aagcggcctc ggttggctgg ccgcacacct gcaagccgtt tggccggaca
    11881 ccatcgcccc gctattgaag tcgctgctgg tggatggcgt gatcggcggc gtgggggccg
    11941 tgatttcctt tctgcccaac atcctgctgc tgtttttggc catcgcgttt ctggaagaca
    12001 ccggctacat ggcgcgcgtg gccttcctga tggaccgcat catgcacaag atgggcctgc
    12061 acgggaaaag tttcatcccg atgctgatcg gtttgggctg cacggtgccg gccatcatgg
    12121 ccacgcgcac tctcgaacat aaacgcgacc gtttcaccac cattctggtt tcaccatttg
    12181 tgagctgcgg tgcacgcatg actatttatg cgctgctgat tccagccttt tttgcgcccc
    12241 gctggcgcgg gtgggtgctg tggggtattt acatgactgg catcgtggcg gccatcggca
    12301 ttgcgcgcct gttgcgcggc acgttattcc gcggcgaaac cgcgccgttc gtcatggaac
    12361 tgccgccgta ccggctgccc acgtggcgct cggtgggcat tcacatgtgg gatcgcgcgt
    12421 ggatgtatct gcaaaaagcc ggcaccatca ttttggccat ttcggtgttg ctgtgggcgc
    12481 tgggcacatt cccgcggcgc gtggactata gccgcgacta cgccgcggcg atggcacagg
    12541 ccgccacgcc ggaagccgcc gcggatctgg ccgcgcagca acaggccgag acgctggaat
    12601 acacaatcat cggccgcctc ggcaaagtgc tggagccggt catccggccg ctggggttcg
    12661 attggcgcgt aggcaccgcg ctcgtcggcg cgtttgccgc caaagaagtt ttcgtggcgc
    12721 aaatggggat tgtgttctca ctgggagagg cgggcggcaa cccggatgac ctgcgcgcca
    12781 cgttgcggca gaactatacc ccgctgcaag ggttggccat gatgctcttt tgtttgctga
    12841 gcgcaccgtg catggcgacc atcgccgtga cgcgccgcga aaccgactcg tggggctggg
    12901 cgctgggcat gctgttcggc atgaccatgg tggcgtggat ttgcaccttt gtcgtgtacc
    12961 ggttaggttt gttgttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg
    13081 gcgggtgtgc tgcgcgattg gacccatgcc tgaactccgg gattgcgggc cggtcgcaag
    13141 atggcagaat gggcgcccca tgaagattac cccggcaacc cgtctggaat ggctggccgc
    13201 gcttggcgca gcccttaccg ccggcttgtt gcaagcggtg ttggcgccgc tggaatggac
    13261 cgcgtgcgcg tgggtggcgt tggtgccact gctgatcgtg gcacggctgg taccggggcg
    13321 cctcgcgctc aaaatgggtt tcgtgacggg cgggctcttc tggttgatca gcattcgctg
    13381 gttgacgcaa gtgacggtac tggggtgggt ggcgttgagc gcttattgcg ctctgtattt
    13441 tttgccgccg gtgctggtgg cgaatcgttg gcgcggcggt ggtgggagct ttgccattgc
    13501 ggtggccgtc gcctggagcg ccagcgaatt catccgcggt tggctgggta caggtttccc
    13561 atggaatccg ctgggcgcgg gtctcgcgcc atggttgccg ggcattcaac tggcggaaat
    13621 cggcggtgtc tggctggtgt ctgggctggt ggtggcggtg aacgcgctgc tggcgtgggc
    13681 gttggtggag cagcgccact ggcgttcgct ggcgctcggg gcgctgctgg tggccgccgc
    13741 gctggggtgg gggcagtggc gggtgcatca tctgcccacg ccggcccgcc acatccgcgt
    13801 ggcgctggtg cagacggcga tcccccaaga cgaaaaatgg gttgcggcca aaatccgcat
    13861 gatctatgac cggctcatca cgctcacccg gcaggcgcaa ggcgatccgg atgtggaatt
    13921 ggtcatctgg ccggaaacgg cattgcccga cgatgtccgc aacagcgaag ccagctacac
    13981 cgtggtgtgg agcctttgca ccaacggcac atccatcctg accggcacgc tggatacggc
    14041 cgtgcttgac gacggcaccg agcaatattt caacagcgtt ttcctgtttg ataccgaggg
    14101 tcgcattgtg ggcgaatatg acaagcgtca cttggtgctc ggcggggaag tcattccgct
    14161 ggagcgctgg attcccgacc ggtggatgct ggcgctgggt ctgcccacca gcatcactgc
    14221 cggccagaaa ggcgcggtgt tcactgctgg ccgccagcag gtgccgctgg cgccgttggt
    14281 ttgcttcgag gatattctgc ctcatctggc gcgggccgat gtccgcgccg gcgcgcgcgc
    14341 attggtcaat gtgacgaatg acgcctggtt cgatgaagtg atcgcgccgc gccagcacat
    14401 gcgtaatgcc gtgttgcgcg cggtggaaaa tcgcgtgcca ttgattcgcg cggccaatac
    14461 cggcgtgtcg tgcgtcattg agcccagcgg cgcggtggcg gccatgctct ccgacggggc
    14521 gggggggacc gccggcgccg gcgtgctgtg ggccgatgtg gccgtgccgg acccgaccgc
    14581 gcccttgact tgctatgccc gtttcgggga tatgtacgca ttcgcctgcc tcgccgccac
    14641 gggtctgtgg ctgctggcgg catggcggga atcacgaacc taagaacaga accatgctcg
    14701 acgaactttc cacgcgcatt gccaccgtga aggaaaacct cgcaggaatg agaggttatc
    14761 tttgacgtcg ccggcaaaaa acggctcatt gaagaattga cccagcagat gtccgcgcca
    14821 gatttctggg atgaccagaa cgcggcgcgc gcgctgatcg aaaaaaagaa caccttggag
    14881 gcggtcaccg agccctatga caagctcgta gcgctgcaag aggagttgga agtcatggcc
    14941 gcgctactgg gcgaggccgg cacggactcg cccacacatc cggatgtggt cgaactggcc
    15001 aagcaagttg ccgcgctgga aaaacaatac gcccacgtgg agttgcaatc gctgatggtc
    15061 gggcggttcg atgccaacaa tgccttcctg acgctgcacg ccggcgcggg cggcaccgag
    15121 tcctgcgact gggccgccat gttgctacgg atgtatgatc gctactgcca gcagcagggc
    15181 ttcgaagtca ccacagtgga ttaccaaccc ggcgacgaag ctggagtcaa gagcgtcacc
    15241 ctgatggtca gcggtccgtg ggcctacggt tttttgaagg cggagcgcgg tgtgcaccgc
    15301 ttggtgcgga ttagcccttt cgacgccaac aaacggcggc acacctcatt cgcctcgctc
    15361 gatgtcgtgg cggaaatcag cgacgacatt gaagtcacca tcgaggacaa agacctgcgc
    15421 gtggatacct accgctccag cggcgccggc gggcagcacg tcaataaaac cgacagcgcc
    15481 attcgcctga cccacctgcc cacgggcatt gtggttgctt gccaggccga gcgcagccag
    15541 ttagccaacc gcaacaaggc catgaaaatc ctgcgcgcca aaatctacga actggagcag
    15601 gacaaaaagc gccgcgaaat ggaaaagttc tacggcgaaa aaggcgagat cgcctggggc
    15661 caccaaatcc gatcgtacgt cttgcagccc tacaccatgg ccaaggatca ccgcaccgac
    15721 gtagaaacca gccaagtctt cgaggtgctc gacggtgact tgggtctctt tatcgaagcc
    15781 tggctcaaac aaaaccgc
//
LOCUS       DAOPNP010000053        15125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4661_length_15125_cov_7.569608, whole genome shotgun sequence.
ACCESSION   DAOPNP010000053 DAOPNP010000000
VERSION     DAOPNP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15125
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4661_length_15125_cov_7.569608"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1251
                     /locus_tag="PKI64_06250"
     CDS             277..1251
                     /locus_tag="PKI64_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE00649.1"
                     /translation="MRNFVTSMVLACCLLAATGTFGQGEGTTFDSKYNAVLSKLQSMG
                     YGYYSAKEWEDIDRAVQELVTDAAQRDDGDAFVKAVVIKAMVLSDMRRQHADAVNELV
                     AARKKVAKMAGVDASKLFVKQAEVLAEAGDSAGVQNVIAEYKASKYYNPKPYAWSGMT
                     QPGDPLLVARPNATTGDSLPLTIMEHAVIRAKSAPGVIFPDATLTDINGRSFTLSSLR
                     GKVVLVDFFARGWKVWEENLPQVQDLWTRYHPQGFEVVSICLEPNAVGLESAGLPWSV
                     VAGAPSITRPLGIFGQTTSYLLDQNGVIIARDLRGQDLAFAVRHALSK"
     gene            1269..1841
                     /locus_tag="PKI64_06255"
     CDS             1269..1841
                     /locus_tag="PKI64_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOE00650.1"
                     /translation="MASPVFHGLAGAGLTLLLAGGDPLPPFASTGRRWLALGAGGVVA
                     CLPDVDYLPGLWRGYLNTTHQQMTHSLVWTVLVAAGLWLLGRAWRPALFGRRMLGLLL
                     VLLGSHLLIDGVTADRLAPYGFPLWAPFSFRPVLASFSWFPAWQKTALADLWHWHNVW
                     ALTREVVFGLLLLGACAWVKRGWTHRQPAL"
     gene            1849..2697
                     /gene="lpxI"
                     /locus_tag="PKI64_06260"
     CDS             1849..2697
                     /gene="lpxI"
                     /locus_tag="PKI64_06260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOE00651.1"
                     /translation="MNQLHPDEVPTTLGVIAGRGSYPRQLARSAHAQGVQRVVAFAFK
                     GETERAIMRDADEVVWIRLGCLGELLDAIKARGITNMVMAGQIMPTRLFTLRLDAKAL
                     AVLKTLPKRNAHTIFGAIGNELQAIGVNLLPAYRFMETEMPAAGLLGQRAPDEREQAD
                     IELGAQVAKATSGLEIGQTVVIKEGTVLAVEGFEGTDETILRAGRLGGAGGVVVKVAK
                     LGHDMRFDIPIIGTRTFKMLRKARISCLAVEAGRTILLEREKLVAEANRLGIAFTAFS
                     LDRIVP"
     gene            2712..3668
                     /locus_tag="PKI64_06265"
     CDS             2712..3668
                     /locus_tag="PKI64_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE00652.1"
                     /translation="MSTQPTKIKTGVIGVGSLGQWHARIYSEMPDVELVGVYDADRKR
                     AQEIAERYHTRAFASIAELTAAADALNVVVPTDKHREVAGQIIAQGRHVLVEKPIAAS
                     TAEAEELVALAQEHNVILQVGHVERFNPVLTAINPAKQKAHYIEALRIAPYPPPRFGL
                     LPRGTEVSVILDLMIHDLEIILHLVQSKVVDIRAVGVPVLSKSEDIANVRLRFENGCI
                     ANVTASRISTEKQRKIRVFLDDSYVSLDYQEQTGKILRKKLLGISQEQIPIHKGEPLA
                     LELRSFVDCIRSRANPLVSGAHGAEALKLAVAICQSIRQGPT"
     gene            3668..4804
                     /gene="lpxB"
                     /locus_tag="PKI64_06270"
     CDS             3668..4804
                     /gene="lpxB"
                     /locus_tag="PKI64_06270"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOE00653.1"
                     /translation="MKRVLIIAGEVSGDQHAARLVRDLKKVRTDVEWFGIGGEALRAE
                     GVNTWVDLDQMAVVGFWEVLVRYPFFRRIFKQVTQRLKREKPDAVLLVDYPGFNLKVA
                     EKAHELGIPVFYYISPQVWAWRPGRIPKMAQILNLLMVIFPFEVGVFKGTGLRTEFVG
                     HPLVESVQRTLATPSAPLPWPDGKIYVALLPGSRRLEIKRVLPPMLAAAREIRRRHPD
                     TVYLVAAANDEIAALIRAQIEALPAADQQSIAVVVGQVRDVVRQARAAMVCSGTATIE
                     TALLKCPMIVVYRTNPLTYLFGQHLVKVPWLGMVNLIANRQLCPEFIQSYAQPIAMAN
                     ALEPLLENSFARTVQLSGFEEVAHKLTGTPRKNAGQLVAEALGD"
     gene            4907..4983
                     /locus_tag="PKI64_06275"
     tRNA            4907..4983
                     /locus_tag="PKI64_06275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4941..4943,aa:Met,seq:cat)
     gene            complement(5053..5457)
                     /locus_tag="PKI64_06280"
     CDS             complement(5053..5457)
                     /locus_tag="PKI64_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE00654.1"
                     /translation="MTFPIVPDLLSSLFKKPNTNPFPAPQLPPTVTGLLDEVNQGKAE
                     LNPPVPAPERLRGKMTYNRETCIGCNLCIKVCPAHAIEAIPAVKKVRFYVSQCIQCGQ
                     CTEICPKQCLALSGEFLNASHDRYDRSLIVER"
     gene            complement(5472..6479)
                     /locus_tag="PKI64_06285"
     CDS             complement(5472..6479)
                     /locus_tag="PKI64_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOE00655.1"
                     /translation="MSVNEILLAAGGALIMAFFSMVGGLWFAGIDRVLAARMQARIGP
                     PVRQPFLDIAKLMIKQNVVPANAIPWLFNGAPVVALASAVTILLYIPMAGIPGLPILQ
                     GHGDLVLIMYLLILPALAMVAGGLAAGSPYATIGAQREMVTMIAYELPLAATIIAFAW
                     KFASLGIEHPFSLATVAAQPVWTLVGPVGFMGLLLLLVTMLLVVPGELGRIPFDAPEA
                     ETEIAGGLLVEYSGRNLALFTLSLAVKTIVVTSLIVGLFFPWNLTDILAVACCATTAA
                     MINLAFFILKLLVVMFFSVTLVRISVARFRINQVVDVYWKILGVLALLGMFLVMADAL
                     F"
     gene            complement(6486..7739)
                     /locus_tag="PKI64_06290"
     CDS             complement(6486..7739)
                     /locus_tag="PKI64_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOE00656.1"
                     /translation="MKTIEVKKPSGYFTQIGPAHPALKEPVMFNLDVEGERIIKADFA
                     PGMSHRGIEWMGLRRNCIQVIYLAERICGICGITHSISFVRAVEQIAQIQVPVRAHYI
                     RSIVGELERIHSHLLWAGVAAHELGFDSAFYLAWRVRENVMDLVEYLTGNRVHYAMIQ
                     IGGVRRDITREQFPRIEESMKFFDNAYDQLARVFLDDTTVQMRCRDCGVLTYEEARQL
                     CPVGPTARASGIKKDVRVDHPYAGYADLDIEYMTPDRLTGEVRGDVYDRIVVRLLEVK
                     QSIALVRQLLEQMPDGPLLAIPKPAVLLAKLKGAAGEAVGRHEAPRGEVMHYVRLEAG
                     KDEPVSWKVKASSYSNYLSWIPMMEGEQIADIPIIAASIDPCISCTDRVALVQDGNRR
                     VLTKAELHRLSVEKTRRIQASLSAR"
     gene            complement(7763..8350)
                     /locus_tag="PKI64_06295"
     CDS             complement(7763..8350)
                     /locus_tag="PKI64_06295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOE00657.1"
                     /translation="MKLLTPQEIADTLQAAAGAGFISARCTEWAEGAKGRKSRQVWLH
                     ITREALLPTLRRLADIHYPHVSVIAHADTGAQVDLMYHFYIYWGIPREEILVTLTISL
                     DKSDLRTPTITGIIPGALTSEREKQEMLGIEVVDIPDGRRLFLPEDFPEGVYPWRKDE
                     TGIPPDMVKELWATGRDSLQLDDTPPAPTPEPPTA"
     gene            complement(8347..8778)
                     /locus_tag="PKI64_06300"
     CDS             complement(8347..8778)
                     /locus_tag="PKI64_06300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOE00658.1"
                     /translation="MKLSQTLGKSLWAFLFNSGSCNGCEIETVATVTPRYDLERFGIK
                     VVGSPRHADILLISGPVTRKMAPRLRRVYEQMPDPKIVLCIGACGTSGGAFYDSYTLV
                     GPVDQVVPVDMYVPGCPPRPEAIIDGVVKILARLEAAEKSA"
     gene            complement(8844..9185)
                     /locus_tag="PKI64_06305"
     CDS             complement(8844..9185)
                     /locus_tag="PKI64_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOE00659.1"
                     /translation="MDTSQTLLTGYGAWNPLLWVVAFAVVLVVALLIRSRGRKDYRED
                     SGLKPFISGNEEPAAGGSHIPASNMYWGFLESLKGYYARLVPLHTGVPTDYVAWVLGV
                     MAVMVLVTLAG"
     gene            complement(9190..10758)
                     /locus_tag="PKI64_06310"
     CDS             complement(9190..10758)
                     /locus_tag="PKI64_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOE00660.1"
                     /translation="MMSTMIMRHLPALLIATPLFAAFLMPLVGAAGRWVRTAWVFLGA
                     ALTATVGLLLAWQVFTTGPVLYVFGAGDPTQTLPGDAAFPIRIMFAVDALSALMIVIA
                     SCSSLAITLYSLNAEARQSGLETFYAMLFLLVTGVLGMVATGDLFNFFVFLEISSLAG
                     AALVAYRIDEGVAVEAGLKYALLSTVGALAVLIAIGILFAQYNALNIAALASRLQFTV
                     LDKVALVLLVVPLAMKCGAVPMHFWTPDSYTTAPSSVTAFLVVSSQASLYGLFRILYT
                     LYGSLLSWATFGWFLIILGVLSMFIGVTMAIPQKDVKRLMAYHAVSQTGYMLLGVGVG
                     LAVLGNRAALEEFGKTAMAGGLFHIFNHAMYKGLLFLTAGAVFYRTGTRNLNKLGGLG
                     HTMPWTMVFFMIGALAIAGVPPFNGFASKLLIYESVFRFSPFLAVVAMVVSILTLASF
                     VKVFHSMFLGARRAEYAAAREVPAAMWVGMALLAVVVMAAGLVPRPFIDHLITPAVNA
                     LCDRAGYIATIFGG"
     gene            complement(10758..11114)
                     /locus_tag="PKI64_06315"
     CDS             complement(10758..11114)
                     /locus_tag="PKI64_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOE00661.1"
                     /translation="MMTRLLLDLPFFTVVLLVVIGLGAMVLKRNLIKILMGLSILESA
                     VNLFLVAIGYRQDGVAPIFTNVPSLHMVLPTVQALTLTSIVIGVATSAMMLSFAMILY
                     KKYGTVDARDIRKLKG"
     gene            complement(11111..11578)
                     /locus_tag="PKI64_06320"
     CDS             complement(11111..11578)
                     /locus_tag="PKI64_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOE00662.1"
                     /translation="MKMTKIVRTTADVFYPFCLAFGLYVVMHGHLTPGGGFQGGAVMA
                     TGAALLIVARSYEDITGRLRKGVLKVCEATGLMLFLLAGLSGLLKGLPFMANWLTQTG
                     GWVGKVMEFGPNGGELATGGLIPLLNVGVGIEVLGGLSIILLYMISGTKEKKP"
     gene            complement(11565..11849)
                     /locus_tag="PKI64_06325"
     CDS             complement(11565..11849)
                     /locus_tag="PKI64_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00663.1"
                     /translation="MKTAVLYAILLILFGGLLYIVTDLPFGQPCKADMDEYFIRNSQT
                     QTGANNVVTSVVFDFRGFDTLGEATVLFTAVLGVGMMFRKLHEGEEYEND"
     gene            complement(11846..12103)
                     /locus_tag="PKI64_06330"
     CDS             complement(11846..12103)
                     /locus_tag="PKI64_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOE00664.1"
                     /translation="MTAFLVLQALVLLGLLATAVMVVAFKDLLAAAIASAAFSLLLAL
                     EFYILQAPDVAIAEAGIGAGLTTAIFIIAIRGTKRHEEHDQ"
     gene            complement(12100..12453)
                     /gene="mnhG"
                     /locus_tag="PKI64_06335"
     CDS             complement(12100..12453)
                     /gene="mnhG"
                     /locus_tag="PKI64_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOE00665.1"
                     /translation="MILNVLIWVALGIGLFFNTLGVIGILRFPDVYTRLHADTKATTF
                     GSIFTSIAVVLYAAGQYWHTLDGQYATLALHTVIAVVVLAITNATGAHAIARAAYRRG
                     HRPVQAVVDHLEGKP"
     gene            complement(12450..12704)
                     /locus_tag="PKI64_06340"
     CDS             complement(12450..12704)
                     /locus_tag="PKI64_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOE00666.1"
                     /translation="MSIWMFTVSVLCALMLLAMVRLVRGPTAADRVVALDAINTLVVA
                     SMIVLGVAFKQIIFIDVAIIYALLSYVGTLFIAKYLGGEL"
     gene            complement(12701..13231)
                     /locus_tag="PKI64_06345"
     CDS             complement(12701..13231)
                     /locus_tag="PKI64_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOE00667.1"
                     /translation="MLAFIVTALFSGAVYLLLTAGSGSVLHFWAPTELVVACFVALAV
                     GLVARKYFAHARQRAVLNPVRWVLGVAYAVGPFFLEMAKANVDVAYRVITGRIRPGIM
                     RVKSGMKTDVGTLMLANSITLTPGTLTVDIDEETNDLYVHFINLADETKAREFLEAEE
                     VFGLFNCPAWVRRIAE"
     gene            13546..14958
                     /gene="ahcY"
                     /locus_tag="PKI64_06350"
     CDS             13546..14958
                     /gene="ahcY"
                     /locus_tag="PKI64_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOE00668.1"
                     /translation="MAKAKKTLDAIVKDLSLADFGRKEIELAEHEMPGLMSVRAEFAK
                     TKPLKGARIAGSIHMTIQTAVLVETLQALGARVRWASCNIFSTQDHAAAALAVKGTPV
                     FAVKGETLKEYWEYTDRILDWGDESPNMILDDGGDATLFVHLGVKAVREGLQTLAGPY
                     DSTEEEILKDQVRRAVKKNPERFLKMAKTIRGVTEETTTGVHRLYQMAERKELLFPAF
                     NVNDSVTKSKFDNIYGCRHSLVDGLMRATDVMIAGKVAVVAGYGDVGKGSCQSLKGQC
                     ARVIVTEVDPICALQAAIDGYEVMTMDEACTLGDIFVTATGCCDVITEKHMLKMKDLA
                     IVCNIGHFDSEIQTEAMKKYRWEEIKPQVHRIWTRKNKSLLLLAEGRLVNLGCATGHP
                     SFVMSNSFSNQTLAQIELFTNHDKYPIGVYTLPKKLDEKVARLHLAHLGVRLDRLNRK
                     QAAYLGVPVDGPYKPNHYRY"
BASE COUNT         3152 a         4792 c         4509 g         2672 t
ORIGIN      
        1 ccattctgat ggaggattgt ccttggatca tcaccggcca tgcgcgcgcg ttcaatgtgc
       61 ggcaagcccg actgcaagac tatcagcgcc atgatttttc gtggggcaag gagaagtatt
      121 ggcgcctggc gccgaccgct ccttgaaccc gtcgcgcggt tcgacatccg ccgcgcccaa
      181 aagcccgatt ttggcggggt aaagccgcgc acaaaaagaa ttgtcttttg tccgcgggtc
      241 ggcacaatgg ccgcgtagtt ttttggagga aacaacatgc gtaacttcgt gacaagtatg
      301 gttctggcct gctgcctgct ggccgcgacg ggaacatttg gccaagggga aggcaccacg
      361 ttcgacagca aatacaatgc cgtgctgagc aagttgcaga gcatgggcta tggctactat
      421 tccgccaagg aatgggagga cattgaccgc gcagtgcaag aactggtgac cgatgccgcg
      481 cagcgcgatg acggcgatgc gttcgtgaaa gccgtggtca tcaaggcgat ggtgttgagc
      541 gatatgcgcc gccagcacgc ggatgccgtc aatgaactgg tggcggcccg caagaaagtg
      601 gccaagatgg ccggcgtgga tgcgtccaaa ctttttgtga agcaggcgga agtgctggcc
      661 gaagccggcg acagcgcggg cgtgcaaaac gtcattgcgg aatacaaagc cagcaagtac
      721 tacaacccga aaccctatgc gtggtcgggc atgacgcagc ccggcgaccc gctgctggtg
      781 gcccggccca atgccaccac gggcgactcg ctgccgttga ccatcatgga gcacgcggtc
      841 attcgcgcca agagcgcccc gggcgtcatt ttccccgatg caaccttgac cgatatcaac
      901 ggccgctcgt tcactttgtc cagcttgcgc ggcaaggtgg tgctggtgga tttctttgcc
      961 cgcggctgga aggtgtggga agagaatctg ccgcaagtgc aggacctctg gacgcgctat
     1021 catccgcagg gctttgaagt cgtgagcatt tgtctcgaac ccaacgcggt cggcttggaa
     1081 tcggccggtt tgccgtggtc cgtggtggcc ggcgcgccaa gcatcacgcg cccgctgggg
     1141 attttcggcc agaccaccag ctatttgctg gatcaaaacg gcgtgatcat cgcgcgtgat
     1201 ttgcgcggcc aggatttggc atttgcggtg cggcacgcgc tgagcaaata gccggtgggc
     1261 gagcgggcgt ggcctctcca gtctttcatg gattggccgg cgccgggctg accttgttgc
     1321 tggcgggagg ggaccccctc ccgccttttg catccacagg ccgccggtgg ctggcgttgg
     1381 gcgcgggcgg cgtggtcgcc tgcctgccgg atgtggatta cctgccgggc ctctggcgcg
     1441 gctatctgaa caccacgcat cagcaaatga cacatagtct ggtatggacc gtattggtgg
     1501 cggcgggcct ttggctgttg gggcgtgcgt ggcggccggc actgttcggc cggcggatgc
     1561 tgggactgct cctcgtgctg ctggggagcc atctgctgat tgatggggtg actgccgacc
     1621 gcctcgcgcc ttatgggttc ccgctgtggg cgccattttc gttccggcca gtgttggcgt
     1681 ccttctcgtg gttccccgcg tggcaaaaaa cagcgctggc cgatttgtgg cattggcaca
     1741 atgtgtgggc gctgacgcgc gaagtggtgt tcggtcttct tttgttgggc gcgtgtgctt
     1801 gggtaaagag aggctggacg caccgccaac ccgcgttatg atgaaaagat gaatcagttg
     1861 cacccagacg aagtgcccac caccttgggc gtgattgccg gccgcggctc ctatccgcgc
     1921 caactggcgc gctccgccca cgcgcaaggg gtgcaacgcg tggtggcctt cgctttcaag
     1981 ggcgaaaccg agcgcgccat catgcgcgat gccgatgagg tggtctggat ccgcctgggc
     2041 tgtctcggcg agctgcttga cgctatcaaa gcgcgcggca tcaccaacat ggtgatggcc
     2101 ggccaaatca tgcccacgcg gctgttcacg ctccggctgg acgccaaagc attagccgtg
     2161 ctcaagaccc tgcccaagcg caacgcccac actattttcg gcgccattgg caacgagttg
     2221 caggccatcg gcgtcaacct gctgcccgcg tatcgtttca tggaaactga gatgccggcc
     2281 gctggcctgt tgggtcagcg cgcgccggat gagcgcgagc aggcggatat tgagttgggc
     2341 gcgcaagttg ccaaagccac cagtgggctg gaaattggcc aaacggtggt catcaaggaa
     2401 ggcaccgtgt tggcggtgga aggctttgaa ggcaccgatg aaaccatcct gcgcgccggc
     2461 cgcctcggcg gggcgggtgg ggtagttgtc aaagtcgcca agctgggcca tgacatgcgc
     2521 ttcgacattc ccatcatcgg cacgcgcacg ttcaagatgc tgcgcaaggc ccgcatctcc
     2581 tgcctggccg tggaagccgg tcgcaccatc ttgctggagc gggaaaaact ggtggccgaa
     2641 gccaaccggc tcggcattgc ttttaccgct ttttcattgg atcgcattgt cccctaacgc
     2701 gcgaggtcgc catgtcaacc caacccacca aaatcaaaac cggcgttatt ggagtcggca
     2761 gcctgggcca gtggcacgcg cgcatttact ccgaaatgcc cgatgtggaa ctggtcggtg
     2821 tgtatgacgc ggaccgcaag cgcgcacagg aaattgccga gcgctaccac acccgcgcgt
     2881 ttgccagcat cgccgaattg accgccgccg ccgatgcgct gaatgtggtg gtgccgacgg
     2941 ataaacaccg tgaagtggcc ggacagatca tcgcccaagg ccgccacgtg ttggtggaaa
     3001 aacccattgc ggccagcacc gcagaagccg aggaattggt ggccttggcc caggagcaca
     3061 atgttatttt gcaggtgggc catgttgagc ggttcaaccc cgtgctcacc gccatcaatc
     3121 ccgccaagca aaaagcccat tacattgaag ccctgcgaat cgcgccatat ccaccaccgc
     3181 gctttgggct gctgccccgc ggcaccgaag taagcgtgat cctcgacctg atgatccacg
     3241 atctggaaat cattttgcac ctcgtgcaat ccaaggtggt ggacatccgc gccgtcggcg
     3301 tgcccgtgct gagcaaaagc gaggacatcg ccaacgtgcg cctgcgtttt gaaaacggct
     3361 gcattgccaa cgtcaccgcc agccgcatca gcaccgaaaa gcagcggaaa atccgcgtgt
     3421 ttctggatga ctcctatgtc tcgctcgatt atcaggagca gaccggcaaa atcctgcgca
     3481 aaaaattgct ggggatttcc caggaacaaa ttccgattca caaaggcgaa ccgctggcgt
     3541 tggaattacg gtcgttcgtg gactgcattc gcagccgcgc caatccgctg gtcagcggcg
     3601 cgcatggcgc cgaggccttg aagctggccg tggccatttg tcagagcatc cggcagggac
     3661 ccacctgatg aagcgtgtgt tgatcattgc cggcgaagtc tcgggcgacc agcatgccgc
     3721 ccgcctcgtg cgcgacttga aaaaagttcg caccgatgtc gagtggttcg gcatcggcgg
     3781 ggaggcgctg cgcgccgaag gggtcaacac ctgggtggac ttggaccaga tggccgtggt
     3841 ggggttctgg gaagtgctcg tgcgctatcc gtttttccgc cgcatattca agcaagtgac
     3901 ccagcgcctg aagcgcgaaa agcccgacgc ggtgctgctg gtggattatc ccggcttcaa
     3961 tctgaaagtc gccgaaaaag cccatgaatt gggcattccg gtattttatt acatcagccc
     4021 gcaggtttgg gcgtggcgcc cggggcgcat ccccaaaatg gcgcaaatcc tcaacctgtt
     4081 gatggtgatt ttcccgtttg aagtcggcgt cttcaagggt acgggcctgc gcacggaatt
     4141 cgtggggcac ccgttggttg aatccgtaca gaggaccttg gccacgccct cggcgcccct
     4201 gccgtggccg gacggcaaaa tttatgtggc tctgctgccg gggagtcgtc ggctggaaat
     4261 caaacgcgtg ctgccgccca tgctggcggc ggccagggaa atccggcgcc gccatccgga
     4321 tacggtgtat ctggtcgcgg cggccaacga tgaaatcgcc gcgttgatcc gggcccaaat
     4381 cgaggcgcta ccggcggcag accagcaatc catcgcggtt gtggtggggc aagtgcgtga
     4441 tgtggttcgt caagcccgcg ccgccatggt gtgttccggc acggccacca tcgagaccgc
     4501 cctgctaaaa tgccccatga ttgtggtgta ccgcaccaat ccgctgacct acctgttcgg
     4561 ccagcacttg gtgaaagtcc cttggctggg catggtcaac ctgattgcca accgccagct
     4621 ctgcccggag ttcatccaga gctatgcgca gcccatcgcc atggccaatg cccttgaacc
     4681 gcttctggaa aactcattcg cgcgcacggt gcaactctcc ggttttgaag aagttgccca
     4741 caagttgacc ggcacacccc gaaagaatgc cggccaactt gtcgcggaag ccctcggcga
     4801 ctaatccgac gccgccggca ccgcgcaaat aaaacggata aaaccgtttt ttgcgcttgc
     4861 attcccggcc caaggtcgta tgctacgggt gcaatttgaa acagattgcg gggtggagca
     4921 gcccggcagc tcgtcaggct cataacctga aggtccttgg ttcaaatcca agccccgcta
     4981 ccatcttggg ctccaaacta cggtttggag ccttttttcg tgcccgccgg cgggacgact
     5041 ggccgcctca atctatcttt cgacgatgag ggagcggtcg tagcggtcgt gggaggcgtt
     5101 gaggaactcg ccggagaggg cgaggcattg cttggggcaa atctcggtgc attgtccgca
     5161 ctggatgcat tgcgagacgt aaaaacgcac tttcttgacg gccgggatgg cctcgatggc
     5221 gtgagcaggg cagaccttga tgcacaggtt gcagccgatg caggtttccc gattataggt
     5281 cattttgccg cgcagccgtt ccggggcggg cacgggggga ttgagttcgg ccttgccttg
     5341 gttcacctcg tccaacagtc ccgtaacggt aggcggcagt tggggggcgg ggaaggggtt
     5401 ggtgttaggc ttcttgaaca ggctgctcaa cagatcgggc acaatgggga aagtcatcgc
     5461 aaattctccg gttagaacag ggcgtcagcc atcactaaaa acattccgag gagcgccagc
     5521 acgcccagga ttttccaata tacatccacc acttggttga tgcggaagcg ggccacggaa
     5581 atccgcacca gcgtgacgga gaagaacatc accaccagca gtttcaggat gaagaatgcc
     5641 agattgatca tggcggcagt ggtggcgcag caggccacag cgaggatatc cgtgaggttc
     5701 cacgggaaga acaagcccac gatcagcgag gtgaccacaa tggttttgac ggccagcgag
     5761 agggtgaaca gcgccagatt gcgtcccgaa tattccacca gcaggccgcc ggcaatttcc
     5821 gtttcggctt cgggggcgtc aaaggggatg cggcccaatt cgccgggcac caccagcagc
     5881 atggtcacca gcagcagcaa caggcccatg aaacccaccg ggcccacaag cgtccagacg
     5941 ggctgcgcag ccacggtcgc cagcgaaaac ggatgctcga tgccgaggct ggcaaacttc
     6001 cacgcaaacg cgatgatcgt ggcggcgagg ggcagttcat aggcgatcat ggtgaccatt
     6061 tcgcgctggg cgccgatggt ggcatagggc gatccggccg ccaacccgcc ggccaccatg
     6121 gccagcgcgg gcagaatcag cagatacatg atcagcacca agtcgccatg gccttgcagg
     6181 atcggcaagc cggggatgcc ggccatgggg atatagagca ggatggtcac agccgaggcc
     6241 agcgccacga ccggcgcgcc attgaacagc cacgggatgg cattggccgg caccacgttt
     6301 tgcttgatca tcaatttggc gatgtccaga aacggctggc ggacgggcgg gccgatacgc
     6361 gcctgcatcc gggcggccaa aacacggtca atgccggcaa accagagccc gccgaccatg
     6421 ctaaagaaag ccatgatcaa cgcgccgccg gcggccagaa gaatctcgtt cacgctcatg
     6481 gggtcttacc tcgcggaaag cgacgcttga atgcgccggg ttttttccac cgacagccgg
     6541 tgcagttcgg ccttggtcag cacgcgccgg ttgccatcct gcaccagggc gacgcggtcg
     6601 gtgcaggaga tgcacgggtc aatggaggcg gcaatgatcg ggatatcggc gatttgttcg
     6661 ccctccatca tgggaatcca cgacagatag ttggagtagg acgaggcctt gaccttccag
     6721 gagacgggtt catccttgcc ggcttccagg cgcacatagt gcatgacttc gccgcgcggg
     6781 gcttcgtggc ggcccacggc ttcgccggct gcgcctttga gcttggccag cagcacggcc
     6841 ggcttgggaa tggccagcaa cggcccatca ggcatttgct ccagcagttg ccggaccagc
     6901 gcgatggact gcttgacttc cagcaggcgc accacgatgc ggtcatagac gtcgccgcgc
     6961 acttcgccgg tgagccggtc aggcgtcatg tattcaatgt ccaaatccgc gtagccggcg
     7021 taggggtggt ccacgcgcac atccttcttg atgcccgagg cgcgcgcggt ggggcccacg
     7081 ggacacaact ggcgggcttc ttcataggtc agcacgccgc agtcgcggca gcgcatttgc
     7141 acggtggtgt catccagaaa aacgcgagcc aactggtcgt aggcgttgtc gaagaacttc
     7201 atggattcct cgatgcgcgg gaattgctcg cgggtgatgt cgcgccgcac gccgccgatc
     7261 tggatcatcg cgtagtggac gcggttgccg gtgaggtact ccaccaaatc catgacgttt
     7321 tcgcgcacgc gccaagccag ataaaatgca gaatcgaagc ccagctcgtg ggcggccacg
     7381 ccggcccaaa gcaggtggga gtggatgcgc tccagctcgc ccacgatgga gcggatatag
     7441 tgcgcgcgca ccggcacctg gatttgcgcg atctgctcca cggcccgcac gaacgaaatc
     7501 gagtgcgtaa tgccgcagat gccgcaaatc cgctcggcca gatagatcac ctgaatgcaa
     7561 ttgcggcgca ggcccatcca ttcgatgccg cggtgggaca tccccggtgc gaaatcggcc
     7621 ttgatgatgc gctcgccctc gacatcgagg ttgaacatga ccggttcctt aagcgcgggg
     7681 tgcgccggac cgatttgcgt aaagtagccg gagggttttt tgacttcgat ggttttcata
     7741 cgcgtgcctg cagcggggtg ggttatgcgg tggggggctc cggggtgggg gcggggggag
     7801 tgtcgtccag ttgcaaggaa tcgcggccgg tggcccagag ttccttgacc atgtcgggcg
     7861 gaattccggt ttcatccttg cgccaaggat atacgccttc cgggaaatct tcgggcagga
     7921 agagccggcg gccatcgggg atgtccacga cttcgatgcc gagcatttcc tgtttttcgc
     7981 gttcgctggt gagcgcgccg gggatgatgc cggtgatggt gggcgtccgc aggtcggact
     8041 tgtccagcga aatggtgagg gtgacgagga tttcttcgcg cggaatgccc cagtaaatgt
     8101 agaagtgata catcaggtcc acttgcgcgc cggtgtcggc gtgggcaatg actgagacgt
     8161 ggggataatg gatatccgcc agccgccgca gcgtgggcag gagcgcctcg cgcgtgatgt
     8221 gcagccacac ctgccggctc ttgcggccct tggcgccttc ggcccattcg gtgcaccgcg
     8281 cggaaataaa gcccgcgccg gcggcggctt gcagggtgtc cgcgatttct tggggagtga
     8341 ggagtttcat gctgatttct ccgcggcttc gaggcgggcc agaatcttga ccacgccgtc
     8401 aatgatggct tcggggcggg gggggcaacc gggcacgtac atgtccaccg ggaccacttg
     8461 atccacgggt cccaccagcg tgtaggagtc gtaaaacgcg ccgccggaag tgccgcacgc
     8521 gccgatgcac aacacgattt tcggatcagg catttgctca tacacgcggc gcaggcgtgg
     8581 cgccatcttg cgcgtgaccg gcccgctaat cagcaggatg tcggcatggc gcggcgagcc
     8641 gaccaccttg atgccgaagc gttcgaggtc atagcgtggc gtgaccgtgg ccacggtttc
     8701 aatttcgcac ccgttgcacg acccggaatt gaacaggaag gcccacaggg atttgcctaa
     8761 agtttgggaa agtttcatcg tttgccgttc gctagtttct gttcatccat tcttcactca
     8821 tcatccgccc gggaagggga gcctcagccc gccagcgtca ccaataccat caccgccatg
     8881 actccgagca cccaggccac ataatcggtg ggcacgcccg tgtgcagcgg caccagccgc
     8941 gcgtaatagc ccttaagcga ctccaaaaat ccccagtaca tgttggaggc cggaatatgg
     9001 ctgccgcccg ccgccggctc ttcgttgcct gaaataaacg gcttgaggcc ggaatcttcg
     9061 cggtaatcct tgcgcccgcg ggagcggatc agcagcgcca caaccaacac cacagcgaac
     9121 gccaccaccc acagcagcgg attccatgcg ccgtagccgg tcaacagggt ttgtgaggta
     9181 tccatgcgct catcctccga aaatcgtggc gatataaccg gcgcggtcgc acaacgcatt
     9241 gactgccggc gtgatcaaat ggtcaataaa cgggcgcggc accagcccgg cggccatcac
     9301 caccacggcc agcaacgcca tgcccaccca catggccgcc ggcacttcgc gcgccgcggc
     9361 atattccgcg cggcgcgccc cgaggaacat ggagtggaac accttcacaa acgaagccaa
     9421 cgtcaggatg ctgaccacca tggccaccac cgccaggaac ggcgagaagc ggaacacgga
     9481 ttcatagatc agcagcttgg aggcaaaccc gttgaacggc ggcaccccgg cgatggccag
     9541 cgcgccgatc atgaagaaca ccatcgtcca cggcatggta tggcccagcc caccaagttt
     9601 gttgaggttg cgcgtgccgg tgcggtagaa caccgcgccg gcggtcagga agagcagccc
     9661 cttgtacatg gcgtggttga aaatgtggaa caggccgccg gccatggccg tcttgccgaa
     9721 ctcctccagc gccgcgcggt tgcccagcac ggccagcccc acgcccacgc ccagcagcat
     9781 gtagccggtt tgcgataccg cgtggtaggc catcaggcgt ttcacatcct tttgcggaat
     9841 ggccatggtc acgccgatga acatggacag cacgcccaga atgatcagaa accagccgaa
     9901 cgtggcccag ctcagcagcg aaccgtagag cgtgtagaga atgcggaaaa gtccgtagag
     9961 gctggcctgg ctggagacca ccagaaacgc cgtcaccgag gagggcgcgg tggtgtagga
    10021 gtccggtgtc caaaagtgca tgggcaccgc gccgcacttc atggccaacg gcaccaccag
    10081 cagtaccaac gccaccttgt ccagcaccgt gaactgcaag cgcgacgcca gcgccgcgat
    10141 gttcagcgcg ttgtactgcg cgaacaaaat gccgatggca atcaacaccg ccaaggcgcc
    10201 caccgtgctc aacagcgcgt acttcaggcc ggcctcgacg gccacgcctt catcaatccg
    10261 gtacgccacc agcgccgcgc cggccagcga gctgatttcc aggaacacga agaagttgaa
    10321 caagtcgccc gtggccacca tgcccagcac gcccgtcacc aacagaaaga gcatggcata
    10381 gaacgtctcg aggccggatt gccgcgcctc ggcattcagg gaatacagcg tgatcgccag
    10441 cgatgagcac gacgcaatca caatcatcag cgcgctcagc gcgtccaccg cgaacatgat
    10501 gcggatgggg aaggcggcat cgcccggcag ggtttgggtg ggatcgcccg cgccgaacac
    10561 atacagcacc gggccggtgg tgaacacctg ccacgccaac aagaggccca ccgtggcggt
    10621 caacgccgcg cccaaaaaca cccatgccgt ccgcacccag cggcccgccg cgcccaccag
    10681 cggcatcagg aaggcggcaa acaggggcgt ggcaatcaac agcgccggca aatgtcgcat
    10741 gatcatcgtg ctcatcatca acctttgagt ttccggatgt cccgggcatc caccgtgccg
    10801 tacttcttat acaggatcat cgcaaacgac agcatcatgg ccgaggtcgc cacgccgatg
    10861 acaatggacg tcagcgtgag cgcctgcacc gtcggcagca ccatgtgcag cgagggcaca
    10921 ttggtgaaaa tgggcgccac gccatcctgc cggtagccaa tggccaccaa aaagagattc
    10981 acggccgact ccagaatgga gaggcccatc aggatcttga tcaagttgcg cttgaggacc
    11041 atggccccga ggccgatcac caccagcagc accaccgtaa aaaacggtaa atccaggagc
    11101 aggcgcgtca tcatggcttc ttctccttgg taccggaaat catgtagagc aggatgatgc
    11161 tcaacccgcc cagcacctcg atccccacgc ccacgttcag cagcgggatc aagccgccgg
    11221 tggccaactc gccgccgttg gggccgaatt ccatcacctt gcccacccag ccgccggttt
    11281 gggtcaacca gttggccatg aacggcagcc ccttgagcag gccgctcaag cccgccagca
    11341 ggaacagcat caggccggtg gcctcgcaga ctttgagcac gcccttgcgc aggcggcccg
    11401 taatatcctc atagctgcgc gccacgatca gcagcgccgc gccggtagcc atcaccgcgc
    11461 cgccctggaa gccgccgccc ggcgtcaggt ggccgtgcat caccacatac agcccgaagg
    11521 cgaggcagaa gggataaaac acatcggcgg tcgtccgcac gattttagtc attttcatat
    11581 tcttcccctt cgtgcagctt gcggaacatc atgcccacgc ccagcactgc cgtgaacagg
    11641 acggtggcct cgccgagggt gtcgaacccg cggaagtcga acaccacgct ggtcaccacg
    11701 ttgttggcgc ccgtctgggt ctggctgttg cggatgaagt attcatccat gtcggccttg
    11761 cacggctggc cgaacggcaa atccgtgacg atgtaaagca acccgccaaa caggatcagc
    11821 aagatcgcgt agagaacggc agttttcatt ggtcgtgctc ctcatgccgc ttggtgccgc
    11881 ggatggcgat aatgaaaatg gccgtggtca accccgcgcc aatgcccgcc tccgcaatcg
    11941 ccacatccgg ggcctgcaaa atataaaact ccagcgccag caacaggctg aatgccgccg
    12001 aggcaatggc cgccgccagc aaatccttga acgccaccac catcacggcc gtggccagca
    12061 gccccagcag caccaacgcc tgcaacacca gaaacgcggt catggcttgc cctccaaatg
    12121 gtccaccacg gcctgcaccg ggcggtggcc gcgccgatag gccgcgcggg cgatggcgtg
    12181 cgcacccgtg gcattggtga tcgccagcac caccacggca atcaccgtgt gcagcgccag
    12241 cgtcgcatac tggccgtcca gcgtgtgcca gtattgtccc gcggcataaa gcaccaccgc
    12301 gatggacgtg aagatgctcc cgaatgtcgt cgccttggtg tccgcgtgca ggcgcgtata
    12361 gacatccgga aaacgcagaa tgccaattac gcccagcgtg ttgaaaaaca gcccgatgcc
    12421 cagcgccacc caaatcagca cgttgaggat cacagctcac ctcccagata tttggcaatg
    12481 aacagcgtcc ccacgtagga cagcagcgcg tagatgatcg ccacatcaat aaagatgatc
    12541 tgcttgaacg ccacgcccag cacaatcatc gaggccacca ccagcgtgtt gatggcgtcc
    12601 agcgccacca cgcggtcggc cgccgtgggc ccgcgcacca gccggaccat cgccagcagc
    12661 atcagcgcgc acagcaccga caccgtgaac atccagatgc tcattccgca atcctcctga
    12721 cccaagccgg gcagttgaac aacccgaata cctcttcggc ctcgagaaac tcgcgcgcct
    12781 tggtctcgtc cgcgaggttg atgaaatgca cgtagagatc gttggtttcc tcgtcaatat
    12841 ccaccgtcaa cgtccccggc gtcagcgtaa tggagttggc gagcatcagc gtgcccacat
    12901 cggttttcat gccgctcttc acgcgcataa tgcccggccg gatgcgcccc gtaatcacgc
    12961 ggtaggccac atccacgttg gccttggcca tttccagaaa gaacggcccc accgcgtagg
    13021 ccacccccag cacccaccgc accgggttca acaccgcccg ttggcgggca tgggcaaaat
    13081 actttcgcgc caccagcccc accgccaaag ccacaaagca ggccaccacc agttctgtcg
    13141 gcgcccaaaa atgcagaacg gagcccgaac ctgccgtcaa cagcagatac accgcacccg
    13201 aaaacaaagc agttacgata aaagcgagca tactttccta tctgaataca aaccaaaccg
    13261 acgctatcaa gtccacgtcg cgctttccaa ccaaaacttt gcgactcgat attgcgcgca
    13321 gagtaaaccg ttttacaccg ctggcaagct ttttttgttt tccgccccgg cgcacgattc
    13381 cggtgcggcc cgatctatcc ccgccgtatc ccaaaagacc gcagggcagg ggagggtact
    13441 cgcctcgctc cacgccgcaa aaccgccatc gcgccgtcac cggacttgcg ccctccggcc
    13501 attgccccta tagtcccgcc tttgtcaatc acaaggagac tcatcatggc caaagcaaaa
    13561 aaaacattgg atgccatcgt caaggacttg tcgctcgccg acttcggccg caaggaaatt
    13621 gaactggccg aacacgaaat gcccggcctg atgagcgtcc gcgccgaatt cgccaaaacc
    13681 aagcccctca agggcgcgcg cattgccggc tccatccaca tgactatcca aactgccgtg
    13741 ttggtcgaaa ccctccaagc tctcggcgcc cgcgtccgct gggccagttg caacatcttc
    13801 tccacccagg atcatgccgc cgccgccctc gccgtcaagg gcacccccgt cttcgccgtc
    13861 aaaggcgaaa ccctcaagga atactgggaa tataccgacc gcattctcga ctggggcgac
    13921 gaatccccca acatgatcct cgatgacggc ggcgacgcca ccctgttcgt gcacctcggc
    13981 gtcaaagccg tgcgcgaagg cctccaaacc ctcgccggcc cctacgacag caccgaagaa
    14041 gaaatcctca aagaccaagt ccgccgcgcc gtcaaaaaga atcccgagcg cttccttaaa
    14101 atggccaaaa ccatccgcgg cgtcaccgaa gaaaccacca ccggcgtcca ccgcctgtac
    14161 caaatggccg aacgcaagga gctgctcttc cctgccttca acgtcaacga ctccgtcacc
    14221 aaatccaagt tcgacaacat ctatggctgc cgccactccc tcgtggatgg cctcatgcgc
    14281 gccaccgatg tcatgatcgc cggcaaagtc gccgttgtcg ccggctatgg cgatgtcggc
    14341 aaaggctcct gccaatccct caagggccaa tgcgcccgcg tcatcgtcac cgaggtggac
    14401 cccatctgcg ccctgcaagc cgccattgac ggctacgaag tcatgaccat ggacgaggcc
    14461 tgcaccctcg gcgatatctt cgtcaccgcc accggctgct gcgatgtcat caccgaaaaa
    14521 cacatgctca aaatgaaaga cctcgccatc gtctgcaaca tcggccactt cgacagcgaa
    14581 atccaaaccg aggccatgaa gaaataccgc tgggaagaaa tcaagccgca ggtccaccgc
    14641 atttggaccc gcaaaaacaa atccctgctc ctgctcgccg aaggccgcct cgtcaacctt
    14701 ggctgcgcca ccggccaccc cagcttcgtg atgagcaaca gcttctccaa ccagaccctc
    14761 gcccagatcg agctcttcac caaccacgac aaatatccca tcggcgtgta caccctgccc
    14821 aagaagctcg atgaaaaagt cgcgcggctg cacctcgccc acctcggcgt acggctggac
    14881 cggctcaacc gcaagcaagc cgcctacctc ggcgtccccg tggacggccc ctacaaaccc
    14941 aatcactatc gctactaaaa ctggcgccac gccgacccgc cgcgcccgcg caccccgcgc
    15001 cggacgcggc gttttttttg gacttgcgcc cctcccgtcc acgggctacc ttctcgcctg
    15061 cgtttcatgg gccggagtgg cggaatggca gacgcaccag actcaaaatc tggcgccttc
    15121 acggg
//
LOCUS       DAOPNP010000054        14964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4723_length_14964_cov_9.378228, whole genome shotgun sequence.
ACCESSION   DAOPNP010000054 DAOPNP010000000
VERSION     DAOPNP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_14964_cov_9.378228"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1248)
                     /locus_tag="PKI64_06355"
     CDS             complement(124..1248)
                     /locus_tag="PKI64_06355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00669.1"
                     /translation="MKIAVLGSKSLPAFAGADRVAENILHHLPADDGRHYHIYVVRTP
                     AQPRPFDYAPNIRVIPVPAMAGKHSRATTFFLLSALHCALVLRPALAHAHDSAFGPFA
                     WILRLLRPRMPILGTFHGNPYERAKWSRFAKWFLRFSEGAFIRACTELTTVARAKVAD
                     VQARSRTPIEFIPNGVNPTPAISQTGLAAQHGLTPGGYLMFAAGRMDPTKGLHHLLDA
                     YERHQPAEPLLVVGGFEGHNQEYSAQLLARCRATPRVVLIPRLLTQPELFELVAGAKL
                     FVFPSEIEAMSMMLLEVISCGVPVLASDIAENTEVTGPDYPWLFRNADAAHLGEQLAA
                     FLRDGPGPEVAALRTRCARDFNWSAIATRYLAIFQRLAPL"
     gene            complement(1253..2383)
                     /locus_tag="PKI64_06360"
     CDS             complement(1253..2383)
                     /locus_tag="PKI64_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE00670.1"
                     /translation="MDLSVILTYRCQSRCSMCQVWQHPSVPAEEIGPATLAKLPNGFR
                     TITLTGGEPTLREDLPELAAVLRPKTRRLVVMTNGLLAEPLAELVARWPDIAIRVSVD
                     GHGAKNDTIRGEENGYARKMATVRHLLAAGARDVGLAVTIQDDNCDQLLALFKMAQSF
                     GGDISLATLHNGFLFHKIDNEPYNRLRTARAIQPLITELLATRRPRFWRQAYFALGLM
                     KKILGQPRGWRCPAGRDFLYLDPWGRLYACPIRPDLEMGDLNIGTWRSLLKSPGAAEA
                     RTRALRCTHNCWLTDHARPALRNHRWPRLPSWRAWRWLVANKVRVTLGHPPPFERDVP
                     FHDMAQGFIAPRRESWLGRPYTPVEQVKTERPYGAFNNVMNK"
     gene            2716..2789
                     /locus_tag="PKI64_06365"
     tRNA            2716..2789
                     /locus_tag="PKI64_06365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2748..2750,aa:Gln,seq:ttg)
     gene            2827..3789
                     /locus_tag="PKI64_06370"
     CDS             2827..3789
                     /locus_tag="PKI64_06370"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOE00671.1"
                     /translation="MQSQFKIFSGSANRPLAEKIAARVGMTLSAAAIDRFPDGEISVT
                     YEETVRGRDVFIVQPMGLQPNEYLMELLIMTDAARRASAKRITAVMPFFAYARQDRKD
                     RSRVPISAKLVANLLVAAGVNRVLTVDLHSPQIQGFFDIPVDHLYAAPLMVRYLREKL
                     PVDNLVVVAPDPGGLKMAYSYSQLLGAGLALGGKHRKSATEVEAFELVGEVAGKDCIL
                     TDDMTTTAGTLCSAARLAKAHGAKSVRAVVSHCPITPLGIQRLKESPIEELITTDTIP
                     QQPGWNGFPITVLTVADLLGDAIKRIYGDESLSKLFEIHQGRTQ"
     gene            3786..4514
                     /locus_tag="PKI64_06375"
     CDS             3786..4514
                     /locus_tag="PKI64_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOE00672.1"
                     /translation="MKETKLTVQRRTQKGSASAGRLRRTGWFPGVVYGEGRPGLDIQL
                     NEHDFVMMLRKHRSENMIVDLAVEGQEKPIKAMLKAMQHHPMTGRVLHVDFYEVSMTR
                     RIQIEVPILLVGTPVGVAKEGGILEHVHRTLEVQCLPGDLIEEFPLDVTELHIGKTLR
                     VRDIPVDAAKYKVLDDPDQVIVAVAAPRTEEEEKAEADAEAATAAAGPEVLTEKKAEE
                     GEEKEKEKDGKAPAAKDAEKKEKK"
     gene            4548..5111
                     /gene="pth"
                     /locus_tag="PKI64_06380"
     CDS             4548..5111
                     /gene="pth"
                     /locus_tag="PKI64_06380"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE00673.1"
                     /translation="MKLVVGLGNPGRAYDQTRHNAGWLVLDEFARRRGAKFRRSWCRP
                     VQTAKTTLEGAGALLLVKPLTYMNLSGNVLPALMRPAGLTAKDLIVVADDVNLPMGKL
                     RIRAQGSAGGHNGLKSVIERLGTEQFVRVRVGVGDKEAGQSLREHVLERMDAAERTAL
                     AETAARAATAVETILLQGVDRAMNEFN"
     gene            5123..5455
                     /gene="rpsF"
                     /locus_tag="PKI64_06385"
     CDS             5123..5455
                     /gene="rpsF"
                     /locus_tag="PKI64_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOE00674.1"
                     /translation="MNKYEAMIIFRESLKETDWDGAVESVRAEIEKLGGKVTSSTRLG
                     RREFARPMQKELSGHYGLLAFQLAGDKVAPLQARLKLDEQVFRVQVVKAPAVPPAPKK
                     EEDHGHAE"
     gene            5439..5882
                     /gene="ssb"
                     /locus_tag="PKI64_06390"
     CDS             5439..5882
                     /gene="ssb"
                     /locus_tag="PKI64_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE00675.1"
                     /translation="MATLNKVMLIGNLTRDPELHRTPSGTAVTTLRLALDDSYTNKSG
                     ERVERAVFLDVDVWDRQAELCQQYLFKGSPVFVEGRLQMDSWDDKESGQKRTRLKVRA
                     ERVQFLGSRREGAGRDSGSVSDAPPAKAPPPPPEPPMDDESDIPF"
     gene            5903..6142
                     /gene="rpsR"
                     /locus_tag="PKI64_06395"
     CDS             5903..6142
                     /gene="rpsR"
                     /locus_tag="PKI64_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOE00676.1"
                     /translation="MKKKTKRTARRDKTEDQGRTRTRLLKADQVVDYRDYEVLRKFMT
                     ERGKMLPGRVTGANAKQQRQVKRAIRRARVMGLLP"
     gene            6165..6647
                     /gene="rplI"
                     /locus_tag="PKI64_06400"
     CDS             6165..6647
                     /gene="rplI"
                     /locus_tag="PKI64_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOE00677.1"
                     /translation="MGKTIEVILLEDVAELGRSGDACRVRAGYARNFLFPQKLAVVMT
                     AGTKRLIEKKRAEAVVRLAQEKEAAQELLRKLEALTVTCAVKTNADGRLFGSVSAADV
                     LAKLAATGVELAKKQLVMYEALKALGEHEVTLHLHPEVRGKLRVNVVAEKVATDAAAE
                     "
     gene            6734..8062
                     /gene="dnaB"
                     /locus_tag="PKI64_06405"
     CDS             6734..8062
                     /gene="dnaB"
                     /locus_tag="PKI64_06405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOE00678.1"
                     /translation="MDAERAVLGCILQDAERVMDLCIRSQVTSDSFYLQTHRLLFEAM
                     LGLHTARKPVDFVTVTALLRDNQQLDTVGGEDELARLIAGTPTTAHAEYYIQLVYESH
                     LLRRVIDTARSVTEDCFKNEQPAEDVLSAAEGAFFALSESRVGGEKNWATLINDEIVQ
                     IEKLISERKGITGLSTGYDKIDEMLSGMQAGDLIVLAARPSMGKTSLALNIAENVALG
                     ARKQPPRAVAVFSLEMSAESLVRRMVCGHARVPAQNLSKGIVSVQEHRQLVTSADALR
                     NAPIYVDPTAGLEALDLRARARRLKRKHDIQFIVVDYLQLMNYSKYAAEGRQRETAAI
                     SAALKAMAKELKVPVMVLSQLSRAPETRDRLAKPKLSDLRDSGAIEQDADVVFLLRRP
                     SKYPADPEASDETLAVVDIAKHRNGPTGEVRMNFTENLMRFESRREGVDS"
     gene            8059..10380
                     /gene="bamA"
                     /locus_tag="PKI64_06410"
     CDS             8059..10380
                     /gene="bamA"
                     /locus_tag="PKI64_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE00679.1"
                     /translation="MSRAWQGWALGLAAVVWGAAVWAVPVNDIRIVPRGPVPVSAEQV
                     RGQISARVGQELDRGALSEDLRALQKSSVYSFAEVRLEQAADGGVILTFHVQGRPVIR
                     TLTVSGADYIGNKKVRNLMEIGSGDRADDAVLGEKSQKVREHYRKEYFPDAKVTWTFH
                     PVAGHPDMTDVDIQVTEGRRAVVRKIRFTGNRHIPARELRKVMAQKQSTWLSWMTSAG
                     MYDPGVLVADREVLRKVFMDKGYLGATVGEPQIAYVNRKKIDITFPVHEGPVYTLAAW
                     RIEGMQSFAERDVVRGVVVNTGAVATLEGLTRGAQNIRDYYGSRGYIKTLVDPRITLD
                     TNRAAAAVMYHVQEGPLAYIQNIEIRGNAQTQDKVIRREIGVAPGEVYNDVKIRASEN
                     RLRNLNYFSFVNSYPEDTVVSNRFNLVFDLEEQRTGQFMVGAGFSSIDNVLGYVELTQ
                     GNFDLFGWPRFTGGGQRLRLRAQIGDSRSDLELSLIEPWFLNRRLSLGLDLFRRDARY
                     LSDDYDQISTGGSLTLGKPLYTIFHRVNWIYGLENIDIRNVETNASDLIKAEGGGRLK
                     SYGTMELIRDTRNNTFVATRGFRGSVSATLAGGPFGADEDTYQFQLRASQYIPLWFDH
                     VLNLRGWTSVIHEYGDSERVPIFDRLFAGGPRTVRAFRYRKVGPKDQNNEALGGRSVA
                     TATAEYTLPVVDKIRFAVFYDAGIVWQGVYEKDNDPDSVAVGDGIFCDGYGLGVRFDF
                     PGFPIQLDYAWPINTDDYQSDSGRFSFTIGYSY"
     gene            10562..11326
                     /locus_tag="PKI64_06415"
     CDS             10562..11326
                     /locus_tag="PKI64_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOE00680.1"
                     /translation="MVPKHFAARRGRWPRRPRPLWERLAAPLGALGLHGALLLLLLQF
                     PVFRAVYGQRPAVEAEWVWLPEVTLLETPVEPPPPEPEMETPPPEPEPEPEPEPEEPP
                     PPAPVVELPPPPPTPPPEDVPVIPLEKPKPKPKPKPKPPPPPPRVVELTPSQQQDIWS
                     EVRTSIVKALRYPPHARQNRLEGVAIVSLTLDDDGNVVDAHLLPPKPAPVLCVAVEQA
                     LRQIGPFPRAGQAIRDRAMPATAEIAIRFELHRPGQ"
     gene            complement(11344..11748)
                     /locus_tag="PKI64_06420"
     CDS             complement(11344..11748)
                     /locus_tag="PKI64_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOE00681.1"
                     /translation="MRIILDANVAIAAVAARGICEAVVELCLERHQLILCEGILAEIS
                     EKLRREIKVAPALISEYLDVLRTNALLLTPEAVPTDACRDPNDLMILGLVAPGKADVI
                     VTGDQDLLVIKKYKNAHILSPRAFWEASRKFR"
     gene            complement(11745..11960)
                     /locus_tag="PKI64_06425"
     CDS             complement(11745..11960)
                     /locus_tag="PKI64_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOE00682.1"
                     /translation="MTTLTIRLPQALKLDLDKLSQHENRAVSDIVRESLRRYIAVEKF
                     RTVRKKILPFAEAQGLLTDDDVFKALS"
     gene            12626..14074
                     /locus_tag="PKI64_06430"
     CDS             12626..14074
                     /locus_tag="PKI64_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00683.1"
                     /translation="MKWTSRLVFGVLLWWGASAGARPAILGVFSNEEGEFKTVTLMVH
                     RSGYAYFHAAVMGTIGEWKYDAKRLELTLTCYDPQANREESLKFAFDAREKAYWIMDP
                     ETGLRATDSAGRLRRLSDKIPPELVAAFAAYPENLRRERAEREAHVRARRAHDEKLTR
                     EQPEYERLLVELGADPRAGLREEYHGVDTPGGRALRQALGDLTRTFPEDVLIDMLEHL
                     PEQNHWNREQIFARPELTTATIEAFWPRAVEWGTHLNYSIVGNLAQHPNTPLAIVEKL
                     ATEPGELPIGAVGVAQDRVVQLAREALVATPPPSAEWFAHIVRVAQAMPKLAYTERGV
                     QLLTELVKHPVFPVELLNGLAEDSRWEIQLAVAAQPYTSGEALARLAKSNLPRVRAAV
                     AQHPNLSAAVLLELAADPAGEVRAHIAQHPATPAEVLVKMQGDAYYDVRANLIRNPNT
                     PLEVLQALANDPNMTIRQAARAELRTRQQPAP"
     gene            complement(14178..14582)
                     /locus_tag="PKI64_06435"
     CDS             complement(14178..14582)
                     /locus_tag="PKI64_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00684.1"
                     /translation="MTKVKWLVLVLLAVGLGLAVGCGSDSDNDDDGGVLPPSGSYAGT
                     WTGRVCGRGLIMTLTQNGTTLSGNFTFTDPTFQGTVSGTVSSTTPPATARLNCGGRDW
                     WFEISFASYNQFTGGFYKPEKGGLVCNVSATK"
BASE COUNT         2986 a         4220 c         5022 g         2736 t
ORIGIN      
        1 cgacttgatc tgcccccata aatagcttcg ggtgtcccat tgttgaactc aggaagagcg
       61 tggaaaaatc aggtccaaac gttccacggt gtggaaaatc acgcccacct gttgcgtggc
      121 gcgttacagc ggggccagcc gctgaaatat ggccaagtag cgcgtggcga tggccgacca
      181 attgaagtcg cgcgcgcagc gcgtacgcag cgcggcgact tccgggccgg gaccatcgcg
      241 caggaatgca gccagttgct cgccgaggtg ggcggcgtcg gcgttgcgga atagccaagg
      301 gtagtcgggg ccggtgactt ccgtattttc cgcgatgtcc gatgccagca ccggcacgcc
      361 gcatgagatg acttccagca gcatcatgct catggcttcg atttccgacg ggaacacaaa
      421 cagcttggcg ccggccacca gttcaaacag ctcgggttgc gtgagcaggc gcgggatcag
      481 caccacgcgc ggcgtggccc ggcaacgggc aaggagttgg gcggagtatt cctggttgtg
      541 tccctcgaag ccacccacga ccagcagggg ttcggctggc tggtgccgtt cataggcgtc
      601 cagcaaatgg tgcagccctt tggtggggtc catgcggccg gcggcaaaca tcagatagcc
      661 gccgggagtc aggccgtgct gggcggccag cccggtttgg gaaatcgcag gcgtggggtt
      721 taccccgttg gggatgaact caatgggcgt gcgcgagcgc gcctgcacat cggccacctt
      781 tgcccgcgcc acggtggtga gttcggtgca ggcgcgaatg aaggcgcctt ccgaaaaacg
      841 taaaaaccat ttggcaaagc ggctccattt ggcgcgctcg taggggttgc catggaaggt
      901 tcccagtatc ggcattcgcg ggcgcagcag gcggagaatc caggcaaacg gcccaaaggc
      961 ggaatcgtgc gcgtgggcca atgccgggcg caagaccaag gcgcaatgca gcgcactcag
     1021 caggaaaaac gtggtggcgc ggctatgttt gccggccatg gcgggcacgg ggatcacgcg
     1081 gatattgggt gcgtaatcga aaggccgggg ctgggcgggg gtgcgcacca cataaatgtg
     1141 gtaatgccgg ccatcgtcgg cgggcaagtg gtgcaggatg ttttccgcca cgcggtcggc
     1201 gccggcaaat gccggcagtg atttgcttcc caaaacggca attttcatgt gctcacttgt
     1261 tcattacgtt gttgaacgcg ccgtaggggc gctcggtttt gacttgctcc acgggggtgt
     1321 atggccgccc cagccagctt tcgcggcgcg gcgcaatgaa cccctgcgcc atgtcgtgga
     1381 atggcacatc gcgttcgaag gggggcgggt gccccaacgt cacgcgcacc ttgttggcca
     1441 cgagccagcg ccacgcgcgc caactgggca accgcggcca gcggtggttg cgcagcgccg
     1501 gccgggcgtg gtcggtcagc cagcaattgt gggtgcaacg cagcgcgcgg gtgcgcgctt
     1561 ccgccgcgcc cgggcttttg agcaaactcc gccaagtgcc gatgttcaga tcgcccattt
     1621 ccaaatccgg ccggattggg cacgcataga gccggcccca cgggtccaga taaagaaaat
     1681 cgcgccccgc cgggcagcgc caaccccgcg gttggccgag gatttttttc atgaggccca
     1741 acgcgaaata agcctgccgc cagaagcgcg ggcggcgcgt ggccagcagc tcggtaatca
     1801 gcggttgaat ggcccgggcc gtgcgcagcc ggttgtaggg ctcattgtca attttatgga
     1861 acaaaaaccc gttatggagc gtggccagcg agatatcgcc gccgaacgat tgcgccattt
     1921 tgaacagcgc cagcaactgg tcgcagttgt catcctgaat ggtgaccgcc aaacccacat
     1981 cccgcgcgcc ggcggccagt agatggcgca ctgtggccat ttttcgcgca tagccatttt
     2041 cttcgccgcg gatggtatca tttttcgcgc cgtggccatc cacggaaacg cggatggcaa
     2101 tgtccggcca tcgggccacc agttcggcca gcggctcggc cagcaggcca ttggtcatga
     2161 caaccagccg ccttgttttg ggccgcaata cggccgccaa ttccggcaaa tcctcgcgca
     2221 gcgtcggttc cccgccggtc agcgtgatgg tgcgaaatcc gttcggcaac ttggccagag
     2281 tggcggggcc gatttcctct gccggcaccg aggggtgctg ccatacctga cacatgctgc
     2341 aacgcgactg gcagcgatag gtcaggataa ccgacaagtc catgcccgcg aacataacca
     2401 aacccccgcc ccgaggcaag ttgcccccgc cccacgcggg gctttacgcc gcgcgccgga
     2461 ccaaggggtc atacttggcg acgttgttct ccacaagtgt catatatatg ataacaaggt
     2521 cggccgtata ggtggggggg gcgcaaaatc gggtggggat ggggattgac ttttggcggg
     2581 gggggggata tggtgcgcgc gtttttgaac gggcctgtcc gggaaaagga tgggccggtt
     2641 tggttctttg tcacggtcgg gcacggcgcc agggttggtt gccggtgcgc gattgaggaa
     2701 tgccatcgaa cgcattgccc gatggtgtaa tggcagcaca cggccctttg gaggccagag
     2761 tctaggttca aatcctagtc gggcaaccat tgcgccagac gacgaaggag ccgcgacatg
     2821 atgatgatgc aaagccaatt taaaatattt tcgggaagtg cgaaccggcc gctggcggag
     2881 aagattgcgg cgcgggtggg catgaccttg agcgcggcgg cgattgaccg tttcccggat
     2941 ggcgaaattt ccgtgacgta tgaggaaacg gtgcgggggc gggatgtatt cattgtgcag
     3001 ccgatggggt tgcagccgaa tgaatacttg atggaattgc tgatcatgac ggacgcggcg
     3061 cggcgggcgt cggccaagcg gattacggcg gtgatgccgt ttttcgccta tgcgcggcag
     3121 gaccgcaagg atcggtcgcg ggtgccgatt tcggcgaagc tggtggcgaa tttgctggtg
     3181 gcggcggggg tgaaccgggt gctgacggtg gatttgcact cgccgcagat tcagggcttt
     3241 tttgatattc cggtggatca tctgtatgcg gcgccgctga tggtgcgcta tttgcgggag
     3301 aagctgccgg tggataattt ggtggtggtg gcgccggatc cgggcggact gaagatggcg
     3361 tattcctatt cgcagttgct gggcgcgggc ctggcgctgg gggggaagca tcggaagtcg
     3421 gcgacggagg tggaggcgtt tgagctggtg ggcgaggtgg ccgggaagga ttgcatcctg
     3481 accgacgaca tgacgaccac ggcggggacg ctttgttccg cggcgcggct ggcgaaggcc
     3541 catggggcga agtcggtgcg cgcggtggtg agccactgcc ccatcacgcc gctgggcatc
     3601 caacggctca aggagtcgcc gatcgaggaa ttgatcacca cggatacgat cccgcagcag
     3661 ccgggttgga acgggtttcc aatcacagtg ctgacggtgg cggatttgct gggggatgcg
     3721 atcaagcgga tttatggaga cgaatcgctc tccaagttgt tcgagattca tcaagggaga
     3781 acgcaatgaa agagaccaaa ttgactgtcc agcggcggac ccaaaagggt agtgcgtcgg
     3841 ctgggcgcct gcgccgcacg ggctggttcc cgggggtggt gtatggcgag ggccggcccg
     3901 gcctcgacat ccagctcaat gagcacgatt ttgtgatgat gctgcggaag caccgcagtg
     3961 aaaacatgat cgtggatttg gcggtggaag ggcaggagaa gccgatcaag gcaatgctga
     4021 aggcgatgca acaccacccg atgacggggc gggtgctgca tgtggacttc tatgaagtgt
     4081 ccatgacgcg gcggattcaa atcgaagtgc cgatcctgct ggtgggcacg ccggtgggcg
     4141 tggccaagga aggcggcatt ctcgaacacg tgcaccggac gctggaagtg cagtgcttgc
     4201 cgggcgacct gatcgaagaa ttcccgctgg atgtgacgga gctgcatatc ggcaagacgt
     4261 tgcgcgtgcg cgacattccg gtggatgcgg cgaagtacaa ggtgctggac gaccccgacc
     4321 aggtgatcgt ggccgtggcc gcgccgcgta cggaagaaga agagaaggcg gaggccgatg
     4381 ccgaagccgc gactgccgcc gcaggcccgg aagtgctgac cgagaagaag gccgaagaag
     4441 gcgaggagaa ggaaaaggag aaggacggca aggccccggc ggccaaggat gccgagaaga
     4501 aggagaagaa gtaagccgta ccacgcggcg ggaaccggta cccgagcatg aagttggtgg
     4561 ttggactggg caatcccgga cgggcctacg accagacgcg gcataacgcg ggctggctgg
     4621 tgctggacga gtttgcccgg cgccggggcg cgaagtttcg tcgctcttgg tgccgcccgg
     4681 tccaaacggc caaaaccacg ctggagggcg ccggcgcatt gctgctggtg aagccgctga
     4741 cctacatgaa cttgagcgga aacgtcctgc cggcgctgat gcggccggcg gggctgaccg
     4801 caaaagactt gattgtggtg gccgatgacg tcaacctgcc catgggcaag ctgcggattc
     4861 gcgcgcaagg cagcgcgggc gggcacaacg gcctgaagtc ggtgatcgag cgcctgggca
     4921 cggagcagtt cgtgcgggtg cgggttggag ttggagacaa ggaagcagga cagagtttgc
     4981 gcgagcatgt gctggagcgg atggatgccg cggaacgcac ggcgctggcc gagacggctg
     5041 cgcgggcggc gacggcggtg gaaaccatcc ttttgcaggg agtggaccgc gccatgaacg
     5101 agttcaattg aggagacaag cagtgaataa atatgaagca atgatcatct ttcgggagag
     5161 cctgaaggag acggactggg atggcgccgt ggagtcggtg cgcgccgaga ttgaaaagct
     5221 gggcggcaag gtcacgagca gcacccggct ggggcggcgc gagttcgcgc ggcccatgca
     5281 gaaggaattg agcgggcact acggcttgct ggcgttccag ttggcgggcg acaaggtggc
     5341 gccgctgcag gcgcgcttga agctcgatga gcaggtgttc cgcgtgcagg tggtcaaggc
     5401 gcccgccgtg cccccggcgc ccaagaagga agaggatcat ggccacgctg aataaagtca
     5461 tgttgatcgg gaacctgacg cgggatcccg aactgcaccg cacgccctcc gggacggcgg
     5521 tgaccacgct gcggctggcg ctggatgatt cctacacgaa caaaagcggc gagcgcgtgg
     5581 agcgggccgt ttttctggat gtggatgtct gggaccggca ggcggagctt tgccagcaat
     5641 atctgttcaa agggtcgccg gtgtttgtcg aagggcgcct gcagatggat tcgtgggacg
     5701 acaaggaaag cggccagaag cggacccgcc tgaaagtgcg cgccgaacgc gtacagttcc
     5761 tgggcagccg gcgcgaaggc gccgggcgcg actcgggcag cgtcagcgat gccccgccgg
     5821 ccaaggcgcc accgccgccg ccggagccgc ccatggatga tgaatcggat atcccttttt
     5881 aagactggag tagagagcca tgatgaagaa aaagaccaag cggaccgcac ggcgggacaa
     5941 aacagaggac cagggccgga cacggacgcg gctgctcaag gccgaccaag tggtggatta
     6001 ccgcgattat gaagtgttgc gcaagttcat gaccgaacgc ggcaaaatgt tgccggggcg
     6061 cgtgaccggg gcgaatgcca agcagcagcg ccaggtcaag cgggccattc ggcgggcgcg
     6121 ggtaatgggt ttgctgccct aagcggcgaa aggatgagtg tgtgatgggc aagaccattg
     6181 aagtgatcct gttggaagat gtggccgaac tcggccgctc gggcgatgcc tgccgcgtgc
     6241 gcgcgggcta tgcgcggaat ttcctgtttc cccagaaact ggccgtggtg atgaccgccg
     6301 gcaccaagcg cttgattgaa aagaagcgcg cggaagcggt cgtgcgcctc gcgcaggaaa
     6361 aggaagctgc gcaggagctg ctgcggaagc tggaagcgtt gacggtgacc tgtgcggtca
     6421 agaccaacgc ggatggccgc ctgttcggtt cggtaagcgc cgcggatgtg ctggccaagc
     6481 tggccgccac cggcgtggag ctggccaaga agcagttggt gatgtatgag gcgctcaagg
     6541 cgctgggcga gcacgaagtg acgctgcacc tgcatcccga agtgcgcggc aagctgcgcg
     6601 tgaatgtggt ggccgagaag gtcgccaccg acgcggcggc ggaataaggc cggggcgggg
     6661 gatggctggc gcgaaccaca accccgagag cccgcgggct cccgtgccga gcgaccggat
     6721 tcctccgcac agcatggatg ctgaacgggc cgtgctcggc tgcatcctgc aggatgccga
     6781 gcgcgtgatg gacctgtgca ttcgcagcca ggtcaccagc gattcctttt atctgcaaac
     6841 ccatcgcctg ctgtttgagg cgatgctggg gctgcatacc gcgcgcaagc cggtggactt
     6901 cgtcacggtg accgcgctgc tgcgcgataa ccagcaactg gataccgtag gcggcgagga
     6961 tgagctggcc cggctgattg ccggcacgcc caccaccgcc cacgcggaat attacatcca
     7021 gctcgtctac gaaagccacc tgctgcggcg cgtgattgac accgcccgtt cggtgaccga
     7081 ggactgcttc aagaacgagc agccggccga ggatgtcctg agcgccgccg aaggcgcgtt
     7141 tttcgcgttg agcgaatcgc gcgtgggggg cgaaaaaaac tgggccaccc tgatcaacga
     7201 tgaaatcgtt cagattgaaa aactgatttc cgaacgcaag gggatcacgg gcctttccac
     7261 cggttacgac aagattgacg aaatgctctc cggaatgcag gccggcgatt tgattgtgct
     7321 ggcggcacgg ccctcgatgg gcaaaacctc gctggcattg aacattgcgg aaaacgtggc
     7381 gctgggggcg cgcaagcaac cgccgcgggc ggtggcggtc ttcagcttgg aaatgtcggc
     7441 agaatcgctg gtgcggcgca tggtgtgcgg ccatgcgcgg gtgcccgcgc aaaacttgtc
     7501 caagggtatc gtcagcgtgc aggagcaccg ccagttggtg acctcggccg atgccctgcg
     7561 caacgcgccc atttatgtgg accccacggc gggcctcgaa gcgttggacc tgcgcgcccg
     7621 cgcccgccgg ttgaaacgca agcacgacat tcaattcatc gtggtggact acctgcaatt
     7681 gatgaactat tccaagtatg ccgcggaagg tcgccagcgg gaaaccgccg ccatttcggc
     7741 ggcgttgaag gccatggcta aggaactgaa ggtgccggtg atggtgttga gccagttgag
     7801 ccgtgccccg gaaacccgcg accgcctcgc caagcccaag ctttccgacc tgcgcgactc
     7861 cggcgccatc gagcaggatg ccgacgtggt cttcttgctg cgccgcccgt ccaaatatcc
     7921 cgccgacccg gaagccagcg atgaaacgtt ggccgtggtg gacattgcca agcaccgcaa
     7981 cgggccgact ggtgaagtcc gcatgaactt taccgagaat ctcatgcggt ttgagtcgcg
     8041 ccgcgagggt gtagattcat gagccgcgcg tggcagggct gggcgctggg gttggcggcg
     8101 gtggtttggg gcgcggccgt gtgggccgtg ccggtcaacg atatccgcat tgtgccgcgg
     8161 ggcccggtgc cggtcagcgc cgagcaggtg cgcggccaaa ttagcgcgcg ggtggggcag
     8221 gagctggacc gcggcgcgct ttcggaagac ctgcgcgcct tgcaaaaatc cagcgtctat
     8281 tccttcgctg aggtgcggct ggagcaggcc gcggatggcg gcgtcatttt gacgtttcac
     8341 gtgcaggggc ggccggtcat ccgcaccctc accgtcagcg gcgcggatta catcggcaac
     8401 aagaaggtgc gcaacctgat ggagattggc tcgggcgacc gtgcggatga cgcggtgctg
     8461 ggcgagaagt cgcagaaggt ccgcgagcat taccgcaagg aatattttcc ggacgccaag
     8521 gtcacgtgga ccttccaccc ggtggccggc catcctgaca tgacggatgt ggatatccag
     8581 gtgacggaag gccggcgtgc ggtggtgcgc aaaatccggt tcacgggcaa ccggcatatt
     8641 cccgcgcggg agctgcgcaa ggtgatggcg caaaagcagt ccacgtggct gtcgtggatg
     8701 accagcgcgg ggatgtatga tcccggcgtg ctggtggccg accgcgaagt gctgcgcaag
     8761 gtgttcatgg acaagggcta cctgggcgcg accgtcggcg agccgcagat tgcctatgtg
     8821 aaccgcaaga agattgacat cacgttcccg gtgcacgagg gaccggtcta tacgctcgcc
     8881 gcgtggcgca ttgagggcat gcagagcttt gccgagcgcg acgtggtgcg gggcgtggtg
     8941 gtcaacaccg gcgcggtggc cacgctggaa gggctcaccc gcggcgcgca aaacatccgt
     9001 gattactacg gctcgcgcgg ctacatcaag acgctggtgg acccgcgcat cacgctcgac
     9061 accaaccgcg ccgcggccgc agtgatgtac cacgtgcagg aaggcccgct ggcctacatc
     9121 cagaacatcg aaatccgtgg caacgcccaa acgcaggaca aggtcatccg ccgtgaaatc
     9181 ggcgtggcgc ccggcgaggt gtataacgac gtcaaaatcc gcgccagcga aaaccggctg
     9241 cgcaacctca actacttttc cttcgttaat tcctaccccg aagacaccgt cgtctccaac
     9301 cggttcaacc tggtgttcga cctcgaggaa cagcgcacgg gccaattcat ggtgggcgcc
     9361 ggcttctcca gcattgacaa cgttctgggt tatgtggagc tgacgcaggg caactttgat
     9421 ctgttcggct ggccgcggtt caccggcggc ggccagcgcc tgcgcctgcg cgcccagatc
     9481 ggcgactcgc gctccgattt ggagttgtcg ttgattgaac cgtggttttt gaaccggcgg
     9541 ctttcactgg ggctggacct gttccggcgc gatgcccgct acctcagcga cgactacgac
     9601 caaatcagca ccggcggctc attgacgctc ggcaaaccgc tctacaccat tttccaccgc
     9661 gtcaactgga tttacgggct ggagaatatt gacatccgca acgtggaaac caacgccagc
     9721 gacctcatca aggccgaagg tggcgggcgg ctcaagagct acggcaccat ggaacttatc
     9781 cgcgacaccc gtaacaacac gtttgtggcc acgcgcggct tccgcggctc ggtctccgcc
     9841 acgctggcgg ggggaccatt cggcgcggat gaagatacct atcagttcca gttgcgcgct
     9901 tcgcaataca tcccgctgtg gtttgaccac gtgctcaact tgcgcggctg gacgtcggtc
     9961 attcacgaat acggcgactc cgagcgcgtg ccaattttcg atcgcctttt cgccggcggt
    10021 ccgcgcaccg tgcgcgcttt ccgctaccgg aaggtagggc ccaaggatca aaacaacgag
    10081 gcgcttggtg ggcggtccgt ggccaccgcc acggcggaat acacgctgcc ggtggtggac
    10141 aaaatccgtt ttgccgtgtt ctacgatgcc ggcattgtgt ggcagggtgt ctatgagaag
    10201 gacaacgatc ccgacagcgt tgccgtcggc gatggcatct tctgcgacgg ctacggtctc
    10261 ggcgtgcgat tcgatttccc gggcttcccc attcaactcg attacgcttg gcccatcaac
    10321 accgacgact atcagagcga cagcgggcgc ttcagcttca ccatcggcta ttcctattga
    10381 tccgcgtgcg ggccacaccg tggccttgct ccagtgaact tgcgagcgcc tcttggcgct
    10441 gatataccca aggtgcagcg gtatttccgt tgtccggcag caggcaagtt gcccttcctc
    10501 ggcaggaaaa taaacattgg ttaaagttcg caaaaaaaga agcaacggcg ggcggatatt
    10561 tgtggtcccc aagcactttg cggctcgccg tggtcgttgg ccgcgccgac cgcggccgct
    10621 gtgggagcgg ctggccgcac cgctcggcgc gctggggctg cacggcgcgt tgctgttgct
    10681 gctattgcaa ttccccgtgt tccgcgcggt ttatggccag cgccccgccg tcgaagccga
    10741 atgggtctgg ctgccggaag tcaccctctt ggaaaccccc gtggagcctc ccccgccgga
    10801 gccggaaatg gagactccgc cgcccgaacc tgaacccgag cccgagccgg aacctgaaga
    10861 accgccgccc cccgcgccgg tggtggaatt gcccccgccg ccaccaactc cgccgccgga
    10921 agatgttccc gtaattccgt tggaaaaacc caagcccaaa cccaagccga aacccaagcc
    10981 gccgccgccc ccgccgcgcg tggtggaact gacccccagc cagcagcagg acatctggtc
    11041 ggaagtccgc accagcatcg tcaaagccct gcgctacccg ccccacgccc ggcaaaaccg
    11101 gctggagggc gtggccatcg tcagcctcac gctcgatgat gacggaaacg tggtggacgc
    11161 ccacttgctt ccgcctaaac ccgcgcctgt tttatgcgta gcggtggagc aagccttgcg
    11221 gcaaatcggc cccttcccgc gcgccggcca agccattcgc gaccgcgcca tgcccgccac
    11281 cgccgaaatc gccatccgtt tcgaactgca ccgccccggc caataggtgc tgcgtggtga
    11341 ggattatctg aattttctgc tcgcttccca aaacgcgcgt ggggatagga tgtgtgcgtt
    11401 tttgtatttc ttgatgacaa gcaggtcttg atctcccgta acaatcacat ccgccttgcc
    11461 gggggcgacg agaccaagaa tcatcagatc gttgggatcg cgacaggcat cggtgggcac
    11521 ggcttccggg gtcaacagca gggcgttggt ccgcaacaca tcaagatatt cactgatcag
    11581 agcgggcgcg actttgattt cacgccggag tttttcgctg atttcggcca agattccttc
    11641 gcacaagatg agttgatgac gttcgaggca cagctcaaca acggcttcac atattccgcg
    11701 tgcggccaca gccgcgatgg cgacattggc atcgagtatg atcctcacga cagcgccttg
    11761 aagacgtcgt catcggtgag aagtccctgc gcttccgcaa agggcaggat tttcttgcga
    11821 acggtgcgga atttttccac ggcgatatag cgccggaggg attcgcgaac gatatcgctg
    11881 accgcccggt tttcgtgttg gctgagctta tcgaggtcca acttgagcgc ttggggcaag
    11941 cggatggtca atgtggtcat ggggcactct cctgtacgac aatgtagtgc aatgagccgc
    12001 agttgtcaaa accgaaaaaa ccgagtgttt tcactggaaa tcaacaaaat atggtcaaaa
    12061 tgaccgcaaa atagccgatt tccagcgtat ttttgattat tttcccagtt tgggcgcaaa
    12121 aacagccata ttttgactct tcgccgattt tagtgaggtg ttgaggccgg aaactgccat
    12181 gcgggaccgc attgcgcgga aaaataaaca acaattaccc gcaataaatt catccagagt
    12241 ttataaagtg tggatgatgc cttcaaaatg accgattaaa aaacgccata aaaatataca
    12301 taacaaataa tatataaatg agatgtgaat attatcaaat ccatccagag tttataaact
    12361 ctggatgaca tggttcaaat ggcaaaaaag aaggtggtct gacgacggtt gcttccgcat
    12421 ccaaaacgag acgagccagc cgctgcaaac agcgaggaac ccaaaatcgg taaaagatgg
    12481 ccaaaatggc tcaaaaactg ccgtttttgg ggtgtttttt gatggttttt ccggttttgg
    12541 tctcaaaata ccgatttgtg acgttatttc gaggtgcgga tgaggttttc ttgagtggga
    12601 cgggggcggg gggtatggtg gggcgatgaa atggactagc aggctggttt ttggggtttt
    12661 gctctggtgg ggcgcgtcgg cgggggcacg gccggcgatt ttgggcgttt tttccaacga
    12721 ggaaggcgaa ttcaaaacgg tgacgctcat ggtacaccgg agtggctatg cctactttca
    12781 tgcggcggtg atggggacca tcggggagtg gaagtacgat gccaagcgcc tagagctgac
    12841 gttgacttgc tatgatccac aggccaatcg cgaggagtcg ctaaagtttg cgttcgacgc
    12901 gcgggaaaag gcgtattgga taatggaccc ggagaccggg ctgcgagcga cagattccgc
    12961 cgggcggtta cgccggctca gcgataaaat cccgccggag ctggtagcgg cgtttgcggc
    13021 atatccggag aatctgcggc gggagcgggc cgagcgcgag gcgcacgtgc gggcgcggcg
    13081 ggcgcatgac gaaaagctga cgcgcgagca gccggaatac gagcggcttt tggtggaatt
    13141 gggcgcggat ccgcgtgccg gactgcgcga agaatatcac ggcgtggata cgccgggcgg
    13201 ccgagccttg cgtcaggcgt tgggcgattt gacccggacg tttcccgagg atgtgctgat
    13261 tgatatgctg gagcatctgc cggaacagaa tcactggaac cgcgagcaaa tttttgcgcg
    13321 gccggagctg accacagcga ccattgaggc gttttggccg cgggcggtgg agtggggcac
    13381 gcacctgaat tacagcattg tggggaatct ggcccagcat ccgaacacgc cgctggcgat
    13441 tgtggaaaag ctggccacgg agccgggaga actgccgata ggcgcggtgg gggtggcgca
    13501 ggatcgcgtg gtgcaactgg cccgcgaggc gttggtggcc acgccgccac catccgcgga
    13561 atggttcgcg cacatcgtcc gggtggcgca agcgatgccg aagctagcct atacggagcg
    13621 cggagtgcaa ctccttacgg aattggtgaa gcatccggtg tttcccgtgg agttgctgaa
    13681 tggtttggcg gaggattccc ggtgggagat tcagttggca gtggcggcgc agccgtacac
    13741 ctcgggagag gctttggcgc gcttggccaa atcgaatctc ccgcgggtgc gggccgcggt
    13801 ggcgcaacac ccgaatctgt ctgccgccgt cctgctggag ctggcggcgg accccgccgg
    13861 tgaggtacgc gcccacatcg cgcagcatcc ggccacgccg gccgaggtgc tcgtgaaaat
    13921 gcagggcgac gcctactacg acgtgcgggc gaacctgatc cgcaacccga acacaccgct
    13981 ggaagttttg caggcgctgg ccaatgaccc gaatatgacc atccggcagg cggcgcgcgc
    14041 tgaattacgc acgcgccaac agcccgcgcc ctaaccggca ggccaaagag tggcggcgtg
    14101 gcgttcaccc gcaaaaaaac gcgctccgtt gccggggttg ggccgccggc cgcggagcgc
    14161 gctgcaggat gggagggtta cttcgtcgcg gagacgttgc agaccagccc gcctttttcc
    14221 ggtttgtaga aaccaccggt aaactgattg tagctggcga aggaaatctc aaaccaccaa
    14281 tcccgtccgc cgcaattcag ccgcgccgtg gccggtggcg tggtgctgga gaccgtgcca
    14341 ctgacggtgc cttggaaggt ggggtcggtg aatgtgaagt ttcctgacaa cgtggtgccg
    14401 ttttgggtga gtgtcatgat cagtccgcgc ccgcatactc tgccggtcca ggtaccggca
    14461 taactgccgg agggcggcag aactccaccg tcatcatcgt tatcgctatc actgccgcat
    14521 ccgacggcca agcccagtcc aactgccagc agcaccaaca ccaaccattt tacctttgtc
    14581 atgagcgcct cccttgtttt gtttttccag atcgcaagtc agcaacctgc cgacagcaaa
    14641 gcatgtagca cattggcggt gtcggcgcaa tttcaaagcg gcatttactt tgccttttca
    14701 ccccttaaaa atcgaatgta ataaggattg ctttgggggt gtggtgggcc taggattatt
    14761 agcggcccac atatggtttc agccgattca gcccatatca aaccgaaggt ttgcaagacg
    14821 tgtggcaatc ccaaccaccc gttggcggcg caatgccggt attgcggggg gcgtctgcgg
    14881 gtgcggatgg attggttctg cacggtgtgc atcgtgctga ttgcggtgac gctgctggcg
    14941 ctggcgggct acattatgca ccac
//
LOCUS       DAOPNP010000055        14944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4728_length_14944_cov_8.756800, whole genome shotgun sequence.
ACCESSION   DAOPNP010000055 DAOPNP010000000
VERSION     DAOPNP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14944
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4728_length_14944_cov_8.756800"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PKI64_06440"
     CDS             complement(<1..1353)
                     /locus_tag="PKI64_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00685.1"
                     /translation="MNPASQNLQISVIIPNYNYAQFIGEAIESVLAQTVAPLEIIVGD
                     DGSTDHSAAVVAQYADRVRYIQYPHSGETVVRTNLLHETRGEWVVMLDADDRLAPNYL
                     EIMQEKIRAALPDDRLAFAYPDSECFGGRCGRIVRPEFDAQLLKKGNYIVVSSLVRAD
                     VARRFGFDSLFRAGQSDYDFFLTLASHGYRGLHVPEAILHYRIHSASMTSDVLRKSRQ
                     RQIMRRLLRKHRHFFTRDEARTAMTTATQRYWASLIEARSPFAGLGRRTLDLMRFVRA
                     GLNHAELFQQALYALAPAAYHRRRYPPADVFYLFRDTVERRRILAHLPAVDLNRTQLF
                     GFAELQQSGWSMDCNLRLPRISSVRETVAGWIERWYAPRAGVGRGDVMAVRAHLWQMN
                     RAKVVVATTDNTGMPAARLKASGRLKAKLVYISVGLPERMAAVRKISPAREARYRNML
                     S"
     gene            1534..2523
                     /locus_tag="PKI64_06445"
     CDS             1534..2523
                     /locus_tag="PKI64_06445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE00686.1"
                     /translation="MSFWVSVVTPVYNAAEYLRGSVESALQLPEVHEVILAEDGSLDN
                     SLAVCEALVQEHPDRVRLFRHPNGENRGAGLSRNLAIQQARSPFIAFLDSDDLYLPNR
                     FEQDKAILLSQPAIDGVYNALGVHFHTPAGQQWWESSNDRPPSLTRMNSDVHPDELFF
                     NVSPLGRQGHFSVDTLTVRRQVFERIQFSALRLSQDTLFIMQLTALFRLASGNRLTPV
                     ALRGVHGHNRIQDVAGRRAAIELIFMEIFAWLKKEPLTRRQRYALYEAYLRRQHSWAG
                     IQKVLRRCPTLLFNRKYTKHILLRALFRVDPTEPIVPAFFPKWRTRTPHEQPK"
     gene            complement(2531..3610)
                     /locus_tag="PKI64_06450"
     CDS             complement(2531..3610)
                     /locus_tag="PKI64_06450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE00687.1"
                     /translation="MPWIRSGWTLSGHPWFDVAAYPEENRLDRVLARATESERHRLAI
                     EALSIVLDLYVAGYAHRDFHTRNLALVAGHLQLHDFEFLLPFPPNYRSRFSESYDITG
                     QGLPSPARTNNMGFNADRGGRLNLPVIAHAAGISWDAAQRGLADLLRKELLAVSKTFR
                     SDKEGNRHVCKTERIYCSFTVPGLVVRAEEAQRNAARRFARYGMTKSDLTGKRILDLG
                     CHAGGMLFAAQEFQPGQCMGVEYDLNKVRVARRIAAFADLPQVHFQQGDIDRTSPRAL
                     GGEHDVVFCFALERHLTRRHRLCRLLGRVTRDLLLFETNSQTDPAQVERELRRNGFRH
                     VEQLGMCDDDSCPENNVRHLLKAWK"
     gene            complement(3826..5145)
                     /locus_tag="PKI64_06455"
     CDS             complement(3826..5145)
                     /locus_tag="PKI64_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HOE00688.1"
                     /translation="MATPVITIEGIAKRYRRGSISRHTLRDEMRYNWYKWTGRNPADY
                     MGLYGQTAINPEFWALRDVSFDVQQGEVVGILGRNGAGKSTLLKILCRITEPTRGQAT
                     VLGRVASLLEVGTGFHPELTGRENVYMNGTIMGMKKREIDGLFDEIVAFSEIEKFIDT
                     PVKRYSSGMYVRLAFAVAAHLQPEILFIDEVLAVGDVAFQRKCLGKMGSIARDGRTLL
                     FVSHNMAAVQNLCSRCVWLENGAVAAMGNTRDVIHQYLNTQTRGAGEGEGPLYALRGI
                     TERTGNGRIKLTSFAAVDEKGYPLLTVQTGDDVCLRLGYEATKIRPGEMANVTLTIHH
                     LNTQDPLCVCDAIKMGEPFEQVPGSGFFDLHLPRFPLEEGRYRISARVALNDVEADAL
                     PGGIGYLDVAAGDFYGTGRTRPASPVMLNGEWNCEPATAPQVGAASS"
     gene            complement(5156..5992)
                     /locus_tag="PKI64_06460"
     CDS             complement(5156..5992)
                     /locus_tag="PKI64_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00689.1"
                     /translation="MQKTIIRANSPWWRIPWREIVEYRDLIGIMWQRDMLAVYKQSVL
                     GKAWFVLQPLMTTVVFTIVFGNIAGLGTDGVPPLMFYMSGMLIWNFFATSMNSISSTF
                     TGNVPLFSKVYFPRLIVPIVRVLVALATLALNALMFVGFWLFYRYFTEASLPPATRML
                     WFLPILGYTAVLGLAMGLLLASMTIKYRDMQFFLPFFSQLWMYATPIIYPASTVPAQW
                     RWVIQYNPMSWTVEATRWALVGKGTFAAPEVWASAGITSLLLLLGLIMFNRVQRTFVD
                     IV"
     gene            complement(6002..6511)
                     /locus_tag="PKI64_06465"
     CDS             complement(6002..6511)
                     /locus_tag="PKI64_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOE00690.1"
                     /translation="MRASAPDSSGAPVVKRRLVAAAGRLAQEFGFNRAAGEILGSLYL
                     TAGSASLDELEAELGWSKAAVSLAAAQLERMGLVQRVRKPGDRKRYYRSAEDIGTALR
                     DGILKFATARMAGLEAELQQATAALAAVKREPEARFLAARVNRLQDLNRRARRLLGNP
                     LVRLFARLG"
     gene            complement(6575..7696)
                     /locus_tag="PKI64_06470"
     CDS             complement(6575..7696)
                     /locus_tag="PKI64_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOE00691.1"
                     /translation="MEKLWADYSTLWKNSGRCTQTFPHCGKNFSTLWKTPAPRQGAAL
                     VVALWTVLLLSLLVGTMAYEMHVEAGITSFARKRLKAQYAARGGVEYAKFLLAKSFAP
                     SAFEEADEEKEAFRILAKNLSRGIGVSGVEVAAGNARALVDIQPEAGRRNVNKLEDDD
                     WEELLDQAGVPEEKWPDLIDCFMDWTDPGDEHRLNGAEEDDPYYKEKGYAPKNAPLDT
                     VDELLLIKGFTPEIVYGGPPPDPKSPPLRGIAHLLTTFGDGKVNVNTASREVLLTLTA
                     GNGKMMDDWVVADLLKYRLGDDGIPNTKDDGFESVQEAISKSGMDPALADKLSVSDRQ
                     FVRVISIGEDHGVQSGVWAIFEVGNNKVTPIYWREELMQ"
     gene            complement(7918..8781)
                     /locus_tag="PKI64_06475"
     CDS             complement(7918..8781)
                     /locus_tag="PKI64_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE00692.1"
                     /translation="MQRRRTGFTLLELLVALAVFSLAMALAGGAFWGVAKAWTRGGEM
                     LEQLHYGEFVMEQLVTALRGAAWFPSKPSAFGFWLEHGEASWVTSGSAFLPLDSPLRN
                     GLHRVAIGMDTAEGERGLVVRAWPHLMDDMDKRDARPMLASAQVADFDCEWYDFELDT
                     WSHKWEETNSLPKLVRVTLKMKRRQEFKEELVLQRVVELEVAPDLPAREHRDRTTRGD
                     TGRRDVPPGEAPGRQPPTRQPPADDRGLNRPGPAAATNLPPGRQRTAPAWRQPRDRPS
                     VDWQIQRGPRP"
     gene            complement(8781..9218)
                     /locus_tag="PKI64_06480"
     CDS             complement(8781..9218)
                     /locus_tag="PKI64_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE00693.1"
                     /translation="MSRAHRNRAGMTLIEVMLAVVILGMALGVLVGAASRALAVVRQA
                     RNYELARRMIGRVEVESPLRLKDRIEAGTENGSFSGGPAGWSWSRKLEDLGADDEEQE
                     GLFRMTTRVTWAQGEQRSAEEVVQLLYVPKNSSGEFSLKPQVR"
     gene            complement(9215..9787)
                     /locus_tag="PKI64_06485"
     CDS             complement(9215..9787)
                     /locus_tag="PKI64_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE00694.1"
                     /translation="MATRRAGFTLLEILLVVVIIGIAAAVAVPTFARSFRGAKLRAGV
                     RQTLAMHRSAQSKAVLGQRYMAILFDQRKNTLELVEQGGATTKQDSFFGDLQGGSAPG
                     WGQVATGADESAGAPAEDNTPKSQAVRSLQSGVKIVSFRGGKMIDDLYYVTYFPNGMC
                     DGYTLELGDEENRTTTIHVDGVTGKAKVTP"
     gene            complement(9821..10222)
                     /gene="gspG"
                     /locus_tag="PKI64_06490"
     CDS             complement(9821..10222)
                     /gene="gspG"
                     /locus_tag="PKI64_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOE00695.1"
                     /translation="MKTANQEYARKDAGFTLIEILLVVVIIGMLAAVAGIKLTGKMGQ
                     AQRNVAKQGVAVIEQAIDLYEVDNGKLPDSLQNLMTKGNEINWNGPYLKKAEGLKDPW
                     GNEYQYSKSANNYTVISAGPDGAFGTGDDIK"
     gene            10402..13077
                     /locus_tag="PKI64_06495"
     CDS             10402..13077
                     /locus_tag="PKI64_06495"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOE00696.1"
                     /translation="MKMRRLWVAVMLVAGWLSGGRAETDLAIRGSETFGEDLGPKLVA
                     LFLEQYPHVKVELTSLGSASGIADLLDSTCDLAVSSRLFNDDEQRLARSRGLALKYSV
                     AGYCGVGVVVNEANPLQTISDRDIREIFTGRLTNWQQLGGPDLPIVVCIRDASAGTHL
                     GFRMMALNNNTYAANAQVFTGYRALADAVAAQPGAIGYVGMDLLAHPGLHSVAINGIP
                     LTEVTVHEGVYPYVESLLLYTRVKAADPSAERFVQLVRSPAGQEVVRACGFVTADLGP
                     LRANQIFFLLFQVLGGLALFIYGMHVMTRSLRTAAGSSLRSILASATRSRGHGVIFGT
                     VVGFLAHTGAAITMLAGFINAGVMTLEQAIAPVFGANIGTTLSMQLVSFRITDYCWAA
                     IGIGFLLDALIPSERLRKLGDALIGFGLLFLGMETISAGIAPHKDMLAPYLVHIRGDV
                     WTWRLMGVLISALLTALMTSSGAMIGLCFALVKAGVFTRFDQVAVVVLGAHIGTCIVP
                     IMASLSMRIGAWRAAIAHLVFNIANVLLALAAWPLFVWVCEYSAPDNLLRQAANLHTF
                     AMVFATAALLPFTGLFTRLVRGVTPSKEPVPAPSFLDTKLLAKPEQALAAVIRELRRM
                     AEVCVDSMMLNGQLTLSPNRKTYRRLLSNEEIINEVRLSLNDYLERLTQRYLSRRQAL
                     FVQHLDRCMKDIERIGDHLTHIGATSLERFKIPEAIVPEDLFRTWFNLLRSAKRVITL
                     MAKSFDPDANAFQTTALEILRARDAYMILSMDAKAEFAGAARDKRLTPIGGYYLSRYI
                     EDLDRLVRRAKSIAFAERQPDFWLKQTKLERDAKEALAYTIPPLVSSKEYLESLSNDA
                     WDETELMDETPHYIPTESPHLAPPDEQPHPAAPAP"
     gene            complement(13084..14154)
                     /locus_tag="PKI64_06500"
     CDS             complement(13084..14154)
                     /locus_tag="PKI64_06500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00697.1"
                     /translation="MRAIHQIVAGFANGDAISNEARAMREIFRGWGYAAHIYCETRRI
                     LPELRGDARDLATHRDDFRPTDIVLLHLSIGSDVNDIFPTLPGRKVILYHNITPAEYF
                     RGVQEATAHLLARGRQQAAALAGTAEVVLADSQFNATEIAALGHANPQVLPLVLDFSL
                     LRAKPDRRILRQYRDGLVNILFVGRCAPNKRLEDLLNAFYYFQRYVQPLSRLIHVGSH
                     AGLEQYHALLLARARELQLKNVVFAGAVRQAELNAFYQVARVFLCLSDHEGFCIPLLE
                     AMTHDVPVVAYAAGAVPETLAGAGVLLRDKAFDLIAETLGRVAEDEKLRRALLATQRE
                     RLARYERQDLAAELKRLLQPLL"
     gene            14267..14764
                     /gene="tpx"
                     /locus_tag="PKI64_06505"
     CDS             14267..14764
                     /gene="tpx"
                     /locus_tag="PKI64_06505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOE00698.1"
                     /translation="MATVTFHGNPVEVAGSLPAKGSAAPDFTLAKTDLSDVSLKDFAG
                     KKKILNIVPSLDTGVCATSARKFNEAVAKLSDVVLLNISADLPFAAGRFCDSNGLKNI
                     VALSTFRSPGFAQAYGVKITSGPLAGLNTRAIVVLDAANQVVHTQLVPEIGQEPDYAA
                     ALAAV"
BASE COUNT         2824 a         4835 c         4364 g         2921 t
ORIGIN      
        1 cgacaacatg ttgcggtagc gcgcctcgcg cgccggacta atcttgcgca cggccgccat
       61 ccgttccggc aaccccacgc tgatatacac cagtttggct tttagcctac cgcttgcctt
      121 cagccgcgcc gctggcatcc ccgtgttgtc tgtcgttgcc accaccactt ttgcccggtt
      181 catctgccat aggtgcgcgc gcaccgccat cacatcgccg cgtcccacgc cggctcgtgg
      241 cgcataccac cgctcaatcc aacccgccac cgtctcccgt acgctcgaaa tgcgcggcaa
      301 acgcaggtta caatccatgg accagccaga ttgctgcaac tcggcaaaac caaacaattg
      361 cgtccggttc aggtccacgg caggcaggtg ggccagtatc cgacgtcgct ccaccgtatc
      421 acgaaacaga tagaagacat ccgcgggcgg gtaacggcgg cggtggtatg ccgccggcgc
      481 cagggcatac aaggcttgct ggaacaactc cgcgtggttc aagccggccc ggacaaagcg
      541 catcaggtcc aacgtgcgcc gccccaaccc ggcaaacggc gagcgagcct caatcaagct
      601 cgcccaatag cgttgggtcg ccgtcgtcat tgccgtgcgg gcctcgtcgc gcgtaaaaaa
      661 gtggcgatgt ttacgcaata gccggcgcat gatctgccgc tggcggctct tgcggagtac
      721 atcgctggtc atgctggcgg aatgaatccg gtagtgcaaa atcgcttctg ggacatggag
      781 cccacggtag ccatggctcg ccagcgtcag gaaaaagtca taatcgctct gccctgcacg
      841 gaacaaagaa tcaaacccga accggcgggc gacatccgcc cgcaccagcg aactcaccac
      901 gatataattg cctttcttca ggagttgggc gtcaaattca gggcggacga ttcgcccgca
      961 ccggccgccg aagcactcgg aatccggata ggcaaacgcc aagcggtcat ccggcaaggc
     1021 cgcgcgtatt ttctcctgca tgatttccag atagttgggt gccagccggt catcggcatc
     1081 cagcatgacc acccactccc cccgcgtttc gtggagcagg ttcgtgcgca ccaccgtctc
     1141 gcccgaatgt ggatactgga tatagcgcac gcgatcggca tattgggcca ccacggcggc
     1201 ggaatggtcg gtgctgccgt catcccccac aataatttcc aatggcgcca ccgtttgggc
     1261 caacacgctt tcaatggcct cgccaataaa ctgggcatag ttgtaattag ggataattac
     1321 ggaaatctgc aagttttgag acgccgggtt catgaacctc gtgactgtag tctatccgtc
     1381 cttttttctc attcaaaaaa gcgcgcgcgc gaatgtgcgt aatcgaagcc cctccccctg
     1441 gccgcacctg tgtccggccc gttggccgac aagcatcccg ccggcctccc tgccatattt
     1501 gacgaacacg ttggggttgt ttaggttgcc tgcatgtcat tttgggtttc agtcgtcaca
     1561 ccggtttaca atgcggcgga atatctgcgt gggtccgtcg aatccgcgct gcaacttccc
     1621 gaagtgcacg aggtgattct ggcagaagat ggctccctgg acaactcgct ggctgtctgt
     1681 gaagccctcg tgcaggaaca tcctgatcgc gtgcgccttt tccggcatcc caacggcgaa
     1741 aaccgtggcg ccggcctctc gcggaatctg gccatccagc aagcccgctc ccctttcatt
     1801 gcattcctcg attctgatga cctctatctc cccaatcgct ttgaacaaga taaggcgatt
     1861 ctcctttcac agcccgccat agatggggtg tacaatgccc tgggcgtgca ttttcacaca
     1921 ccagcaggtc aacaatggtg ggaatcgtcc aatgatcgtc cgccctccct gacgcgaatg
     1981 aactccgatg tgcatcccga cgaattattt ttcaatgtaa gtccgctggg gcgccaaggt
     2041 catttttcgg ttgatacgct caccgtgcga cgccaggtgt tcgagcggat tcagttttcc
     2101 gcgctgcgtc tttcgcaaga caccctgttc atcatgcagc tcaccgccct cttccgcttg
     2161 gcttccggga atcgcctgac cccggtcgcg ctgcgtggtg ttcatgggca taatcggatc
     2221 caagatgtgg ccggccggcg cgccgccatc gagttgattt tcatggagat cttcgcgtgg
     2281 ctgaagaagg agccactcac ccggcggcag cgttatgccc tctacgaagc ctatctccgg
     2341 cgccagcaca gctgggccgg catccagaag gttctgcgcc gctgtcccac cctgctgttt
     2401 aaccggaaat ataccaaaca cattctcctc cgtgccctct tccgcgttga tcccaccgaa
     2461 cccattgtgc ccgccttttt cccgaaatgg cgaacccgga caccccacga acagccaaaa
     2521 taaaatcgcg ttacttccag gccttgagca ggtgtcgaac attattttcc ggacacgaat
     2581 catcatcaca catgcccagt tgctcgacat gccggaatcc gttgcgccgc aactcccgct
     2641 ccacttgcgc cggatccgtt tgggaattgg tctcaaataa tagtaggtcg cgagtgactc
     2701 gccccagcaa ccggcacaat cggtggcgcc gggtcaagtg gcgctcaagg gcaaaacaaa
     2761 acaccacatc atgctcccca cccagcgccc ggggcgaagt ccgatcaata tccccctgct
     2821 gaaaatgtac ctggggcaga tcggcaaagg cggcgattcg ccgcgccacc cggaccttat
     2881 tcagatcata ttccacgccc atgcattggc ccggctgaaa ctcttgcgcc gcaaacagca
     2941 tgccgcccgc atgacagccc aaatccagaa tccgcttgcc cgtcaaatcc gatttcgtca
     3001 tgccgtagcg cgcaaaacgg cgcgcggcgt ttcgttgcgc ttcctcggcg cgcaccacca
     3061 ggcccggcac cgtgaacgaa caataaatcc gttcggtctt gcagacatgc cggttgccct
     3121 ccttgtcgct ccgaaaggtt ttggaaacag ccaacaattc cttgcgcaac aagtcggcga
     3181 gcccccgctg ggcagcgtcc cacgaaatcc cggccgcgtg cgcaataacc ggtagattta
     3241 gcctgccccc ccgatccgcg ttgaacccca tgttgttcgt gcgagcaggc gacggcagcc
     3301 cctgccccgt gatatcgtag ctctcagaaa aacgggaccg ataattcggg ggaaagggga
     3361 gcaaaaattc aaaatcgtga agttgcaaat ggccggcaac cagcgccaaa ttacgcgtat
     3421 gaaaatcgcg gtgagcataa ccggcaacat acaagtccaa gacaatggac agcgcctcaa
     3481 tagccagccg gtggcgctcg gattccgtcg cgcgggccaa cacccgatcc agccggtttt
     3541 cctcgggata ggccgcaaca tcaaaccaag ggtggcctga gagcgtccat ccggaacgaa
     3601 tccagggcat ggcccaagac gaatcacgga ataggcgcgc ggcttttttt tcagtccaat
     3661 aggctttcca accgtccccg gtgcgatgaa aatatttgcg aaatatgcgc tggccatcct
     3721 gttcgcggat gatcccgaac gaacgccggc cagcgtgcac tacattgggt acatgacgcc
     3781 agcgccgggg cagttcatat tcggcgcttc gtggtgcggg ggcactcatg agctggccgc
     3841 ccccacttgg ggcgcggtgg ctggctcaca attccactcg ccgttgagca tcacaggcga
     3901 tgcgggccgc gtgcggcccg tgccatagaa atcaccggcg gccacatcca gatagccaat
     3961 gccgccgggc agggcatcgg cctccacgtc attgagggct acccgcgcgc tgatgcggta
     4021 gcgcccttct tccagcggga agcggggcag gtggagatca aagaatccgc tgcccggcac
     4081 ctgctcaaac ggctcgccca ttttgatcgc gtcgcagacg cacagcggat cctgggtatt
     4141 caaatggtgg atcgtcagcg tgacgttcgc catctcgccg gggcggattt tggtcgcctc
     4201 atatccgagg cgcaggcaga catcgtcgcc ggtttgcacg gtcagcagcg gatagccttt
     4261 ttcgtccacg gccgcgaagg aagtcaactt gatccggccg ttgcctgtgc gttcggtgat
     4321 accgcgcaac gcatatagcg gcccctcgcc ttcccccgcc ccgcgcgttt gagtgttgag
     4381 atactggtga atcacatcgc gggtgttccc catggcggcc accgcgccat tctccagcca
     4441 cacgcagcgc gagcacaggt tttgcaccgc cgccatgttg tggctcacaa acagcagcgt
     4501 gcggccatcc cgggcaatgg agcccatctt gccaagacat ttgcgctgga aggccacatc
     4561 gcccaccgcc aacacttcat caatgaagag gatttccggt tgcaagtgcg cggccacggc
     4621 aaacgccagg cgcacgtaca tcccactgga atagcgcttc acgggcgtat caatgaattt
     4681 ttcaatttcc gaaaacgcca cgatctcgtc gaaaagcccg tcaatttccc gctttttcat
     4741 ccccatgatg gtgccgttca tatacacatt ttcgcgcccg gtcaattcag ggtggaagcc
     4801 ggtacccact tccagcaggc tggccacgcg ccccaacacg gtggcttggc cccgcgtggg
     4861 ttccgtgatt cggcaaagga ttttcagcag cgtgcttttg cctgcgccgt tgcgcccgag
     4921 aataccgacc acctcgccct gctggacatc gaagctcacg tcacgcagcg cccaaaactc
     4981 gggattgatc gctgtttgcc cgtagaggcc catatagtcc gcgggattgc gcccagtcca
     5041 cttgtaccag ttgtagcgca tttcatcgcg cagggtgtgg cggctgatgc tgccccggcg
     5101 gtagcgcttg gcgatgcctt caatggtgat gacgggtgtg gccatggcgc ggctcctaga
     5161 caatatccac aaacgtgcgt tgaacccggt tgaacatgat caaccccagc agcaggagca
     5221 gactggtgat gcccgcgctg gcccacacct cgggcgcggc aaacgttccc ttgcccacca
     5281 gcgcccagcg cgtggcctcc accgtccacg acatcgggtt atactgaatg acccaccgcc
     5341 actgcgccgg caccgtgctg gcgggataaa tgatcggcgt ggcatacatc cacaattgcg
     5401 aaaaaaacgg caggaaaaac tgcatgtcgc ggtatttgat cgtcatggaa gccagcagca
     5461 ggcccatggc caaacccagc acggccgtat agcccagaat gggcagaaac cacagcatcc
     5521 gggtggcggg cggcaggctg gcctccgtga aatagcggta aaacagccag aagcccacaa
     5581 acatcagcgc attgagcgcc agcgtggcca gcgccaccag cacgcgcaca atgggcacaa
     5641 tcagccgcgg gaagtatacc ttgctgaata gcgggacgtt gccggtgaag gtggacgaaa
     5701 tggaattcat gctggtggca aaaaagttcc agatcagcat cccgctcatg tagaacatca
     5761 gcggcggcac gccgtcggtc cccagcccgg caatgttgcc gaacacgatg gtaaacacca
     5821 cggtggtcat cagcggctgc agcacaaacc atgccttgcc cagcacgctc tgcttgtaca
     5881 ccgccagcat gtcgcgctgc cacatgatgc cgatcaaatc ccggtactcg acgatctccc
     5941 gccagggaat ccgccaccac gggctgttgg cacgaatgat tgttttttgc atgacgattt
     6001 cctatcccag acgggcaaac aggcggacca gcggattccc cagcaggcgg cgcgcgcgcc
     6061 ggttcaggtc ctgcaggcgg ttcacgcgcg cggccagaaa acgcgcctcc ggctcgcgct
     6121 tcacggcggc cagcgcggcc gtggcctgtt gcagctcggc ctccagcccg gccatgcgcg
     6181 cggtggcaaa tttcagaatc ccatcccgca aagccgtgcc gatgtcctcg gcgctgcggt
     6241 aatagcgctt gcggtcaccg ggcttgcgca cgcgctggac caagcccata cgttccagtt
     6301 gcgcggccgc caggctcacc gccgccttgc tccaccccag ctccgcttcc agttcatcca
     6361 gcgaggccga ccccgccgtc agatagaggc tgccaaggat ttcgcccgcc gcgcggttga
     6421 acccgaactc ctgggccaac cgccccgccg ccgccaccag ccggcgtttg acgaccgggg
     6481 caccggatga atctggagca gatgcgcgca tgacaacttt tacaattttg taaaagttat
     6541 gggatttcct gcccgttgtc aagcgggcgc gccgctactg catcaattct tcgcgccagt
     6601 agatgggcgt gaccttgttg ttgccgactt cgaaaatggc ccacaccccg ctttgcaccc
     6661 cgtggtcctc accaatggag atcacccgca cgaactggcg gtcggatacc gacagcttgt
     6721 cggccaacgc cgggtccatg cccgatttgc taatggcctc ctgcacgctc tcgaagccgt
     6781 catccttggt gttggggatg ccgtcatcgc cgaggcggta cttcaacaaa tccgccacca
     6841 cccaatcatc catcatcttg ccgttgccgg cggtcagcgt cagcagcacc tcgcgcgagg
     6901 cggtattcac gttgaccttg ccatcgccga acgtcgtcag caggtgggca atcccgcgca
     6961 gtggcgggct cttgggatcg ggtggcggtc caccatagac aatctccggc gtgaagccct
     7021 tgatcagcag cagctcatcc acggtatcca gcggcgcatt tttcggcgcg taaccctttt
     7081 ccttataata ggggtcgtcc tcctccgccc cgttgagccg gtgttcgtcg ccggggtcgg
     7141 tccaatccat gaaacagtca atcaaatccg gccatttttc ttcgggcacg ccggcttggt
     7201 ccagcagctc ttcccagtcg tcatcttcga gcttgttgac gttgcgccgc ccggcttccg
     7261 gctggatatc caccaatgcg cgcgcgttgc ctgcggccac ctccacgccc gacaccccga
     7321 tgccgcgcga caagtttttg gccagaatgc ggaaagcctc tttttcttcg tcagcctctt
     7381 caaaggcgct cggcgcgaaa ctttttgcca gcaggaactt ggcatactcc acgccgcccc
     7441 gcgcggcata ctgcgccttg agccgcttgc gggcaaacga cgtgattccc gcttccacgt
     7501 gcatttcgta ggccatggtg cccaccagca aactcagcag caacaccgtc cacagcgcca
     7561 ccaccaaggc cgccccctgc cgcggcgcgg gagttttcca cagtgtggaa aagttttttc
     7621 cacagtgtgg aaaagtttgc gtgcagcggc ccgaattttt ccacagtgtg gaataatcgg
     7681 cccaaagttt ttccattgtg tggaaaaatc cacaaaagtt tttccattgt gtggaaaaat
     7741 cgccaaaagt ttttccattg cgtggaaaaa tcggggtcat ttttccacag tgtggaaaag
     7801 ttttttccac agcgtggaaa agtttgcgcg cggcgacccg gatttttcca cagtgtggaa
     7861 atattttttc cacagcgtgg aaaaaccgcg cgccgatttt ccatggtgtg gaaaagatca
     7921 tggccgggga cctcgctgga tttgccaatc cacgctgggg cggtcgcggg gctgccgcca
     7981 agcgggcgcg gtgcgctggc ggccaggcgg cagattggtg gcggcggcgg ggccggggcg
     8041 attcaggccg cggtcatcgg cgggcggctg gcgggtgggc ggctggcggc cgggagcttc
     8101 accgggtggg acgtcgcggc ggccggtgtc cccgcgcgtg gtgcggtcgc ggtgctcgcg
     8161 cgcggggaga tcgggcgcca cttccaattc cacgacgcgt tgcaacacca gctcttcctt
     8221 gaactcttgc cgccgcttca ttttgagcgt cacgcgcacg agcttgggca gggagttggt
     8281 ttcctcccac ttgtgactcc aagtgtccag ttcgaagtcg taccattcgc aatcgaagtc
     8341 ggcgacttgg gcgctggcca gcatggggcg ggcgtcgcgc ttgtccatgt catccatgag
     8401 gtggggccag gcgcggacga ccaagccgcg ctcgccctcg gcggtatcca tgccgatggc
     8461 cacgcgatgc aggccgttgc gcagcggcga atccaacggc aaaaatgccg agccgctggt
     8521 gacccaactg gcttcgccgt gctccagcca gaagccgaac gcggagggtt tggaggggaa
     8581 ccacgccgcg ccgcgcagcg cggtgacgag ttgctccatg acgaattcgc cgtagtgcag
     8641 ttgttcgagc atttcgccgc cgcgggtcca ggccttggcc acgccccaaa acgcaccgcc
     8701 ggccaacgcc atggcgaggc taaaaacagc cagcgccacc agcagctcga gcagcgtaaa
     8761 tcctgtccgg cgccgttgca tcagcgcacc tgcggtttca gcgagaattc gccgctgctg
     8821 tttttgggca cgtagagcag ttgcacgact tcctcggcgg agcgttgttc gccctgcgcc
     8881 cacgtgacgc gcgtggtcat gcggaagaga ccttcctgct cctcgtcatc cgcgccgagg
     8941 tcttccagtt tgcgcgacca gctccagccg gcgggcccgc cgctgaagct gccgttttcc
     9001 gtgccggctt cgatgcggtc cttcaatcgc agcggacttt ccacctccac gcggccaatc
     9061 attcggcggg ccagctcgta attgcgcgcc tgtcgcacca cggccagcgc gcgcgaggcc
     9121 gcgcccacca gcacccccag ggccatgccg aggatcacca ccgccagcat gacttcgatc
     9181 agcgtcatcc cggcccgatt gcggtgcgcg cggctcatgg cgtcaccttg gccttgccgg
     9241 tcacgccatc cacgtggatg gtggtggtgc ggttttcttc gtcgcccagt tccagcgtgt
     9301 aaccatcgca catgccgttg gggaaatagg tcacgtaata caaatcatca atcatcttgc
     9361 cgccgcggaa cgagacgatc ttgaccccgc tttgcaacga gcggacggcc tgcgacttgg
     9421 gggtgttgtc ttcggcgggc gcgccggcgg actcgtcagc gccggtggcc acttggcccc
     9481 agcccggcgc gctgccgcct tgcaggtcgc caaaaaagct atcctgcttg gtcgtggcgc
     9541 cgccctgttc caccagttcc agcgtgtttt tgcgctgatc aaaaaggatg gccatgtagc
     9601 gctggcccag cacggccttg gattgcgcgc tgcggtgcat ggccagcgtc tggcgtaccc
     9661 ccgcgcgcaa cttggccccc cggaacgacc gcgcaaacgt cggcaccgcc accgcggcgg
     9721 caatcccgat gatcaccacc accagcagga tttccagcag ggtgaaaccg gcccgcctcg
     9781 tggccattgc ctgctcctcc gtccgtgcgc gctgccggcc ttatttgatg tcgtcgccgg
     9841 tgccgaaggc gccgtccggc cccgcggaaa tcaccgtgta gttgttggcc gacttgctgt
     9901 actggtattc gttgccccac gggtccttga gcccttccgc ctttttgagg taagggccat
     9961 tccagttgat ctcgttgcct ttggtcatca ggttttgcag gctgtcgggc agtttgccgt
    10021 tgtctacctc atagaggtca atggcctgtt caatgactgc cacgccctgc ttggccacgt
    10081 tgcgctgggc ctgccccatt ttgccggtca gcttgatccc cgccaccgcc gccaacatgc
    10141 cgataatcac gaccaccagc aggatttcga tcagcgtgaa gcccgcatct ttgcgcgcgt
    10201 attcttggtt tgctgttttc atgccatgcc tttccatcac cagattgatg cctgttgcgc
    10261 accataccat atttccggcc cccgccacga agaattatcc ccccccggcc acgtggagca
    10321 tgacgaaaaa taaaaacatg ctagagtgtg cgcaacttgg ctgggtgtcg tgaagaaaaa
    10381 caggagaccg gaaacaacat catgaagatg cgcaggctgt gggttgccgt aatgctggtg
    10441 gcggggtggc tgagtggagg gcgggcggaa accgatttgg ccatccgggg ttcggaaacc
    10501 ttcggcgaag acttgggccc caaactggtc gcgctgtttt tggaacagta tccgcacgtc
    10561 aaggtggaac taaccagcct gggctcggcc tccggcatcg ccgacctgct ggacagcacg
    10621 tgcgacctcg ccgtgtcctc gcgtttgttc aacgatgacg aacaacgctt ggcgcggtcg
    10681 cgcgggctcg cgctgaaata ttccgtggcg ggctattgcg gcgtgggcgt ggtggtcaac
    10741 gaagccaacc cgctgcaaac catcagcgac cgcgacatcc gcgaaatctt caccggacgg
    10801 ctcaccaact ggcagcagct cggcggcccc gacctgccga ttgtggtgtg catccgcgat
    10861 gccagcgcag gcacgcattt gggcttccgc atgatggcgc tgaacaacaa cacctatgcc
    10921 gccaacgcgc aggtgttcac cggctaccgc gcgctggccg acgccgtggc cgcgcagccg
    10981 ggcgccattg gctatgtggg catggatctc ctggcgcatc ccgggctgca ctccgtggcc
    11041 atcaacggca ttccgctgac ggaggtgacg gtccacgagg gcgtgtatcc gtatgtggaa
    11101 tcgctgctgc tctacacccg cgtgaaagcc gccgacccct ccgccgagcg tttcgtgcaa
    11161 ctggtgcgtt cgcccgcggg gcaggaagtg gtgcgggcct gcggctttgt caccgcggat
    11221 ctcgggccgc tgcgggccaa ccaaatcttc ttcctgctgt tccaggtgct gggcgggctg
    11281 gcgctgttta tctatggcat gcacgtcatg acgcgcagcc tgcggacggc cgccggcagc
    11341 tcgttgcgtt ccatcctggc cagtgccacg cgcagccgcg ggcacggcgt gatcttcggc
    11401 accgtggtgg gatttctggc gcataccggc gcggccatca ccatgctggc cggtttcatc
    11461 aatgccggcg tgatgacgct ggagcaggcc attgcgccgg tgtttggcgc gaatatcggc
    11521 accacgctct ccatgcaact ggtgtcgttc cgcatcaccg attactgctg ggcggcaatc
    11581 ggcatcggct tcctgctcga cgcgctgatt ccctccgagc ggctgcgcaa gctgggcgat
    11641 gcgctgatcg ggtttgggct gttgttcctc ggcatggaaa ccatttccgc cggcatcgcg
    11701 ccgcacaagg acatgctggc gccctacctg gtccacatcc gcggcgatgt ctggacctgg
    11761 cgcctcatgg gcgtgctgat ttccgcgctg ttgacagcgc tgatgacctc ctccggggcg
    11821 atgatcggct tgtgcttcgc gctggtgaag gcgggggtgt tcacgcgctt tgaccaggtg
    11881 gccgtggtgg ttctaggcgc gcacatcggc acgtgcattg tgccgatcat ggcctcgctc
    11941 tccatgcgta tcggcgcgtg gcgcgcggcc attgcgcatt tggtgttcaa catcgccaac
    12001 gtcctgctgg cgttggccgc atggccgctc tttgtgtggg tgtgcgaata ttccgcaccc
    12061 gacaacctgc tgcggcaggc cgccaacctg cacacgttcg ccatggtgtt cgcgacggcg
    12121 gcgctcctac cgttcaccgg cctcttcacg cggctggtgc gcggggtcac cccgtccaag
    12181 gagccggtgc ccgcgccgtc gttcctcgat accaagttgc tggccaagcc ggaacaggcg
    12241 ctggccgccg tcatccgtga gctgcgccgc atggccgaag tgtgcgtgga cagcatgatg
    12301 ctcaacggcc aactgaccct ctcgcccaac cgcaaaacct accgccgcct gctctccaac
    12361 gaggaaatca tcaacgaggt ccggctctcg ctgaacgatt acctggagcg gctcacccag
    12421 cgctatctct cccgtcggca ggcgctgttt gtgcagcacc tcgaccgctg catgaaggac
    12481 atcgaacgta tcggcgacca cctgacgcac atcggcgcca cctcgctgga gcggttcaag
    12541 attccggaag ccatcgtgcc cgaagacctg ttccgcacgt ggttcaacct gctgcgcagc
    12601 gccaagcgcg tcatcacgct gatggccaaa tcgttcgatc ccgacgccaa cgccttccaa
    12661 accaccgcgc tggaaatcct gcgcgcgcgc gatgcctaca tgatcctttc catggatgcc
    12721 aaggcggagt tcgccggcgc ggcgcgcgac aaacgcctca cacccatcgg cggctactat
    12781 ctctcgcgct acatcgaaga cctcgaccgg ctggtgcgcc gcgccaagtc catcgcgttc
    12841 gccgagcggc agcccgattt ctggctcaag caaaccaagc tggaacgcga tgccaaggaa
    12901 gcgctggctt acaccatccc gccgctggtg tcgtccaagg aatatctgga atcactcagc
    12961 aacgatgcgt gggacgaaac ggaattgatg gacgaaacgc cccattacat accgacggag
    13021 tcgccgcacc ttgcgccgcc ggacgagcaa ccgcaccccg ccgcgcccgc cccgtgagcc
    13081 aggctagagc aacggctgaa gcagccgctt gagttccgcg gccaagtcct gccgctcgta
    13141 gcgggcgagg cgttcgcgct gcgtggccag cagcgcgcgc cgcaacttct catcttccgc
    13201 cacgcggccg agagtttccg cgatcaggtc aaacgccttg tcgcgcagca acacccccgc
    13261 ccccgcgagg gtctcgggca ccgcgccggc ggcataggcc accacgggca cgtcgtgggt
    13321 catggcctcg agcagcggga tacaaaaccc ctcgtgatcg ctcaggcaca ggaacacgcg
    13381 tgccacttga taaaatgcgt tcagctccgc ctgccgcacc gcgccggcaa acaccacgtt
    13441 tttcaactgg agctcgcgcg cgcgcgccag cagcagggcg tgatattgct ccaggccggc
    13501 gtgcgagccc acgtgaatca ggcgggagag cggctgaacg tagcgctgga aataataaaa
    13561 tgcgttcaac aaatcttcga gacgcttgtt cggtgcgcag cgccctacga agagaatgtt
    13621 gaccagccca tcgcggtatt gccgcaggat gcggcggtcg ggcttggcgc gcagcagcga
    13681 aaaatccagc accaacggca gcacttgcgg attggcgtgg cccagggcgg cgatttccgt
    13741 ggcgttgaat tggctgtcgg ccaataccac ttcggcggtg ccggcgagcg ccgccgcctg
    13801 ctgccggccc cgcgccagca gatgggccgt ggcttcctgc acgccacgaa aatattccgc
    13861 cggcgtgatg ttgtgataga gaatgacctt gcggccgggc aacgtgggaa aaatgtcgtt
    13921 cacgtccgag ccgatggaca ggtgcagcag cacgatatcc gtgggccgga aatcgtcgcg
    13981 gtgggtggcg agatcgcgcg cgtcgccacg cagttcgggc aggatgcgcc gggtttcgca
    14041 gtagatgtgg gcggcgtacc cccacccccg gaaaatctcg cgcatggccc gggcttcgtt
    14101 gctgatggca tcgccattgg caaagcccgc gacaatctga tgaatggccc gcatggttcg
    14161 ttcttaccgt ggcggggcat catataaaat gtgcctgccg ggttgcaaac gcggttttcg
    14221 gttgtaggtt ttcgagaagt acatatagca aaaaaggaga tacaacatgg ctacagtgac
    14281 atttcatggc aatccggttg aagtggcggg ctcgttgccg gccaagggct ccgcagcccc
    14341 cgactttacg ctcgccaaga ccgacttgag cgatgtctcg ctcaaggatt tcgcgggcaa
    14401 aaagaaaatc ctcaacatcg tgcccagttt ggatacgggc gtttgcgcca cctccgccag
    14461 gaagttcaac gaggccgtcg ccaagttgag cgatgtggtg ctgctgaaca tttccgccga
    14521 tctgcccttt gcggcaggcc gcttctgcga cagcaacggg ctgaagaaca ttgtagcgtt
    14581 gtccacgttc cgcagtccgg gctttgccca ggcctatggt gtcaagatta ccagcgggcc
    14641 gctggccgga ctcaacacgc gcgcgattgt ggtgctggat gccgccaatc aggtggtgca
    14701 cacccagttg gtgccggaaa ttggccaaga gccggattac gcggcggcgt tggcggcggt
    14761 ctagcctgaa tcattcacct gagttccgca ttgtacgccc agcaatttcg ttgattcggg
    14821 cttttgtagg gcgggaatgg ttgaggaacg ataccttccc gccgggaacg gctggaaggt
    14881 ctgcgaccat tccagccctt ctatgggggc cgtcaagtca tggcgatgga tttattgtct
    14941 tact
//
LOCUS       DAOPNP010000056        14781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4775_length_14781_cov_7.757300, whole genome shotgun sequence.
ACCESSION   DAOPNP010000056 DAOPNP010000000
VERSION     DAOPNP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_14781_cov_7.757300"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..983)
                     /locus_tag="PKI64_06510"
     CDS             complement(75..983)
                     /locus_tag="PKI64_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOE00699.1"
                     /translation="MDISGLGILEWLQAQRTPAVEAFFSQVTLGGEGFWLLAIAGTVF
                     WLFGARPAYRMGFALAMGDLLAGTLKNLCCVARPWLRNPTIMPNPEAQAGAFGYSFPS
                     GHTTNTALLAGGLAAVVRRWWFWLLAIAWVVMVALSRLVLGVHTPLDVIAGVLIAVPI
                     VWLMGRLYDWTEDNPDKRWLVLTGATVVAAAAWGVIRLRALSIEAGPYFAQDAYGALW
                     GLLAFFAAWHIEREYIRYEPAKLGPYRFVGLVVGLIVLSVMMQHLRRYLAQYVGMDYS
                     YYLTAAAKPLWIFVGCPLLLKGLEKP"
     gene            complement(1015..4347)
                     /gene="ygfK"
                     /locus_tag="PKI64_06515"
     CDS             complement(1015..4347)
                     /gene="ygfK"
                     /locus_tag="PKI64_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOE00700.1"
                     /translation="MSDHFQPLTYDQVAAWVFTELEKRNSIFGIPRELFFQPGAADPF
                     RTALYGQPLETPFGVAAGPHSQMAQNIITAWLTGSRFIELKTIQTLDELAVSKPCIDI
                     QDEGYNVEWSQELKIDQSFTEYLMAWVLIHALQHKLGRAGAPAMIFNMSVGYNLEGIL
                     KPNVQSFLNRMANAGAELETVVAAVAKVYPAVKELAIPSRLSDNVTLSTMHGCPPDEI
                     GRIAAYLIEERGLHTNVKLNPTLLGPEDLRGILNRDLGFRQVTVPDEAFGHDLKYPDA
                     LNILNDLRARAAKKGVQFGVKLSNTLEVINHRRVFDQKEKMMYLSGRPLQAITVNLAA
                     KLAQEFRGDLMMSYAGGADCWNVADLLACGMTTITTSSDLLRPGGYLRTLQYISETSV
                     AMQKAGAGDLNAFILAKAGGKAGDVKAAALANLVAYAATVRQNPLYQRDTYVRQVTKT
                     SRPLGLFDCVKAPCVDICPISQDVPAYMQAVKDGQFDEAAAIVRRDNMMGAALGRACD
                     HICETPCSRVHYDLPLAIREMKRFIMETGRDPAIKVGAPRPGKVAIIGAGPCGISTAG
                     FLREGGVDVTIFDSRPQAGGMATMTLPTYRLMPPVVEQDVKRLEAAGVKFRFGQTAGR
                     DFSLNDLRQQGFDYIVVAAGAMRGVELGIPGQDAEGVLDGITMLRRVWDGELKSLKGR
                     VGVIGGGDVAMDCARVAKRLGGDVTVIYRRTREEMPAHHEELMSLLHEQIPIRELAAP
                     KSVVVKDGKVRGLACATMQLGEPDASGRRRPVEVPNADFTIELDILIAAIGQQPFLDF
                     IKESGIATNRKGYIVADLETGATSVPNVFAGGDAINDGPLSLVKAEGDGKKIAAEILR
                     LIAQVPATPEPMRCTGGLDMVATLKRQATRYPRVEIPELPLAQRNGFAEVIQTMTPEV
                     AQAEAARCLRCDTFCSICTSVCPNQAFLTYDCRPFSADLATWTVQNGQAVAGKPQRFV
                     VLQGVQTAVLTDYCNECGNCVTFCPTAGSPYRDKPRLYASKSEFEAQTDNAFRITGTG
                     AARKISGRFGGVTCTLTPQGAGYMYAAPGWQVQVDANLQPVGAPSVTASADAPLSLVP
                     AAIMSVLLQNIAPEQLPIVAET"
     gene            complement(4593..5645)
                     /locus_tag="PKI64_06520"
     CDS             complement(4593..5645)
                     /locus_tag="PKI64_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00701.1"
                     /translation="MTKWLWLALIPAIAFAGARPYNMRKDPVYIDVHPDRIIVMPEKI
                     EIAESELTVPQNNFECLLEQVASVRNSRYVLLILRPGSEILQHQLRQTIRKYDVDIGV
                     DVWEEGREFSPIELMGIYEMGTADPPKPDPLEHIRMYPVTVEKDRIIFDNDKIVVTRE
                     ELQIPGNPFETLVDRFEANGGNPYIFPYPINSPLVDDLWNVMRKRAPQMVSNYLAKNA
                     TVITPTPIEANTEEKRPVVFECRHNQLFFIRLEQPQATDPETQGYSFDSPREETDEKW
                     FGSQLTELDPDTQYIWFKVRPDSFDIFRKARKVVWDYKLESTCELLGPNDPIQIESKI
                     ITDPQFNHTNEIPTKP"
     gene            complement(5709..7310)
                     /locus_tag="PKI64_06525"
     CDS             complement(5709..7310)
                     /locus_tag="PKI64_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOE00702.1"
                     /translation="MNRTRSTICCKMAGRWGVAFMAIWLAATDAGARVVNLTILNTTD
                     LHGTLRQETIAEAGRGAGSLLQCATLIRRAQADNPHTLLLDCGDIFQGSAASFLTQGR
                     VMATVMNELGYDAFAIGNHEFDWGVETLAAFLGQMRAVPLAANMRVGSGAPLALQRVL
                     PFIIKEVDGIRVAVVGLTTPNIPQWTRGLAQQDISFTDSRRALERTLPLVRAQQPHIM
                     VLLVHQGLLSSDDAANEINTIARRFGEFDAVLGGHLHYVLTGIRVGNVSYAQAGYGAA
                     GVMRVDFAYDTVENRLVDKQFRFVPIDDDVPEDPALAKLVERDLAKATKWLETRVGTT
                     KRDINYSAALPGLCPVQQLLSAAIAAHTGAEVVLHGPLVETGIPAGDIRVADVWRIVP
                     YENTAGCLWLTLGEIQALMEEATEYLGTERYPGAWGLSYDLYPKAPHGHRIRNLRAMD
                     GSAINGKRRLKVALNSYHLAGGGGRFPKIAKMAAMPNARLETSDVVVRDMVLEYIRQQ
                     RVLDIPPGTNATVVLKEPRAWHRRH"
     gene            complement(7307..8581)
                     /gene="clpX"
                     /locus_tag="PKI64_06530"
     CDS             complement(7307..8581)
                     /gene="clpX"
                     /locus_tag="PKI64_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOE00703.1"
                     /translation="MAGKRLPPRSEFCVFCGRERKSVKSMVTGPDDMSICGDCIDVCN
                     AVLARNQGPAGPRPDAARLKVPKPHEIKAKLDEYVVGQERVKKVLAVAVHNHYKRVKF
                     RLAGKTADDDVEIDKSNILMIGPTGSGKTLLARTLARILDVPFSISDATTITEAGYVG
                     DDVENVLLRLIRAADGDVARAEVGIVYIDEIDKIARRTENVSITRDVSGEGVQQGLLK
                     ILEGTISNVPPQGGRKHPQQETIPINTENILFICGGAFVGLDDIIKRRVGKGVIGFDS
                     EKEQRKRLKARHDVHSVEPEDLVKFGMIPEFVGRLPVVATLDDLTEDDLVRILTEPKN
                     AMTRQYQKLLAMEGVKLDFMPAALRELAREAVRRNTGARGLRSLLESIMLDAMYEAPR
                     QRGAKHYVISEKTVTGQENILDTQPLPKEEAG"
     gene            complement(8584..9189)
                     /gene="clpP"
                     /locus_tag="PKI64_06535"
     CDS             complement(8584..9189)
                     /gene="clpP"
                     /locus_tag="PKI64_06535"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOE00704.1"
                     /translation="MNENAYLIPIVVEQTGRGERSYDIYSRLLKERIIFIGSEISDAL
                     ANLVVAQLLFLQSEDATKDVSVYINSPGGSVTAGLAIYDTMQFLKCDVVTYCLGQAAS
                     MAAVLLTAGTKGKRHALPGARIMVHQPWGGTQGTASDIHIQAQEILRLKDYLNGLLAK
                     HTGKSVKDIARDTDRDFFMSAAEAVKYGLVDDVLGAAPAKA"
     gene            complement(9182..10540)
                     /gene="tig"
                     /locus_tag="PKI64_06540"
     CDS             complement(9182..10540)
                     /gene="tig"
                     /locus_tag="PKI64_06540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOE00705.1"
                     /translation="MKVNVENTGPCRKKISVEFSAEEVDNEYGSSLKYYLRHGTVKGF
                     RPGKAPEAMIRRAYDKQILSVLKDHLLATGFQQAMKEHKLQTVAEYDLQQSTLASGQP
                     FSFSLVVDVAPEFELPEYKGLAVDAKKIEIADEAVTQALNDYLKSLGQYEDVTEPLPV
                     ELEDIVAVDYTSTLDGEVPAEMSKELKDLLEATDRWVVANQEYSFLPEFGTQLIGAKV
                     GDTRDITVQFEQDAQPAEVRGKKGVFKTTVKKIRRRKMPVLDEAFLQRIGAKSEEDVR
                     DMFRQLLRQQAEIQEQRRRREQVEAHLLKTVENLDLPESEESEVHNNLVYSIVDENLQ
                     RGVKEQEIRENLDKISASAKEAARTQLKLRYLLKRIADAENLVVTDQEVDSALLRQAQ
                     RDGVSVKDFIRTRKLNEAKLRRDIRTGLRNGKAMDAVLSHAKLTGPGAEQAAQKQEDT
                     HE"
     gene            complement(10660..12579)
                     /locus_tag="PKI64_06545"
     CDS             complement(10660..12579)
                     /locus_tag="PKI64_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOE00706.1"
                     /translation="MIEFRQVSKRYGPQIILDRVDFRLLAGERVGLVGPNGAGKSTVF
                     DLMVGECEADAGRITLAKDIRIGYLRQQLRSGGEQIPVQAYVEQAAPSSATIRHEVQH
                     IEARLATGEDPEARILLRQLGELQTQFELQGGYDVPARTAATLAGLGFHAEELQRPLG
                     EFSGGWQMRAELARALLAEPDLLLLDEPSNYLDVPAVEWLRRRLERFRGTLAMISHDR
                     YLLESLCEITVEVAGGQVTRYPGRYSYYAVEREKRRELALARQANEDRKREQIERFVE
                     RFRAKNTLATRVQSKIKMLEKMERTTVADIARGRREFRLAPPPRSGQEVLRCSGVGFA
                     YEPGQWIFRGVDLAVQRGEKLAVVGPNGAGKTTLLRLMAGQLPPGEGSCTLGHLVVPG
                     YQSQELTETLDRTRTCLDTLKAVAPEEPESSIRTLLGCFGFSGEAVEKRVEVLSGGER
                     IRLAFARILIRPPNLLLLDEPTTHLDVESREALQEALAAYTGTVVLVSHDIAFVRAVA
                     GGIVAVAPAGGGVRRFAGGYDYYCEKIGREAPAASEAAASAETPAKEAQIQAQRARKA
                     QKAEQRKLERRLAVVEEKIAALESGQPELLALLSAAETSPEDRQEAGRRLQDITRQIT
                     ELTAEWDELATRLSG"
     gene            complement(12668..13396)
                     /locus_tag="PKI64_06550"
     CDS             complement(12668..13396)
                     /locus_tag="PKI64_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE00707.1"
                     /translation="MPFRTFNLAEAAEYLHLSAQRLEELARRGEIPCERQGGRLVFRH
                     NQVDEWASQQLLGSSTDKIEDFHRRTTAKYHDLSAEAAIIPALMKPEWIRPEMTCRSK
                     ASVIREMVDLADATGLLCYRDELLESLIEREKLCSTALSGGLALLHPRNHEPYRFEDS
                     FVVLGKTVAQVPFGSPDGTTTDIFFLTCCQNDRIHLHVLARLCMMCHHTSLLLELRET
                     NDPKELYNLLVASEQQVIRLAAKS"
     gene            complement(13416..13808)
                     /locus_tag="PKI64_06555"
     CDS             complement(13416..13808)
                     /locus_tag="PKI64_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00708.1"
                     /translation="MKKTAQQPKKSEPPPPASWRDILSTPAMGETLSTLFGLLFLFQC
                     MILPLVGKAAMQGSGSPGAGPAATVWKNQLFFGVMLLLTMAVGGAAFFAKRLRQQNDG
                     SPFPLFTAGLLGVCALLLVAFATGLLGI"
     gene            13938..14450
                     /locus_tag="PKI64_06560"
     CDS             13938..14450
                     /locus_tag="PKI64_06560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE00709.1"
                     /translation="MSNPQPIIRKARFNDGPAIFKRIKAHPNELVPRPISDIMLNIDR
                     FLVAEANGEIVGTAAWSVLPELDTTKNPSVEIQSVSVREDYQKAGLGRRLVEAALARV
                     AELKPDQVIVLTFTPPFFAKLGFVPVSKEKLMYKLYKGCINCTKYDSPYTCPEEAMAL
                     DFRNWKGNAS"
     gene            14450..>14781
                     /locus_tag="PKI64_06565"
     CDS             14450..>14781
                     /locus_tag="PKI64_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00710.1"
                     /translation="MAGRWRILIAAALLAAGVPAAAWAWAGVQHIQINKLAGRNVPDE
                     MQDFRNFSRPMALPGIFPDLWKEADPDESPRHYFEPDRLPAGFDLKQLSPDLPTALGQ
                     MNIAPEIIG"
BASE COUNT         2813 a         4580 c         4418 g         2970 t
ORIGIN      
        1 aggaaaccga tcctcttttg ccttctcatc ctatctgtgg aaatattttt tccacggtgt
       61 ggaaaagttt gggttcaggg tttttcgagg cccttgagca ggagggggca gccgacaaaa
      121 atccaaagcg gcttggcggc ggcggtgagg tagtaggaat aatccatgcc gacatattgc
      181 gcgaggtagc ggcgcaagtg ctgcatcatg acggacagca cgatcaggcc caccaccagg
      241 ccgacgaagc ggtaggggcc gagtttggcg ggttcgtagc ggatgtattc gcgttcaata
      301 tgccacgcag cgaaaaaggc gagcaggccc cagagggcgc cgtacgcatc ctgcgcaaag
      361 tacgggcccg cttcgatgct cagggcccgc agccggatga cgccccaggc cgcggcggcg
      421 accaccgtcg caccggtcaa caccagccaa cgcttgtcgg ggttgtcttc ggtccagtcg
      481 tagaggcggc ccatcagcca aacgatgggc acggcgatca gcacgccggc gatgacatcc
      541 agcggcgtat gcacgcccag caccaagcgg gacaaggcca ccatgaccac ccacgcaatg
      601 gccagcagcc agaaccacca gcggcgcacc accgcggcca gcccgccggc cagcagcgcg
      661 gtgttggtgg tgtggccgct cgggaatgag tagccaaagg cgccggcctg agcttcggga
      721 ttcggcatga tggtcgggtt gcgcagccaa gggcgcgcca cgcagcaaag gtttttgagg
      781 gtgccggcca gcagatcgcc catggccaac gcaaagccca tgcgataagc cgggcgcgcg
      841 ccaaacagcc agaacaccgt gccggcgatg gccaacagcc agaagccctc gcccccaagc
      901 gtaacttggg aaaagaaggc ttccaccgcg ggggtgcgct gcgcttgcag ccactccaaa
      961 atgccgagtc cactgatatc catgggattt cccgcttggc tcaggagcgg caactcaagt
     1021 ttccgccaca atgggcaact gctccggcgc gatgttctgt aacaacacgc tcatgatggc
     1081 ggcgggcacc aagctcaacg gggcatcggc cgaggcggtc acgctcggcg cgcccacggg
     1141 ctggaggttg gcatccacct gcacttgcca gccgggcgcg gcatacatat agccggcgcc
     1201 ttgcggggtc aacgtgcagg tgacgccgcc aaagcgtccg ctgattttgc gcgccgcgcc
     1261 ggtgccagtg atgcggaagg cattgtccgt ctgcgcctcg aattcgctct tggaagcgta
     1321 gaggcggggc ttgtcccggt atgggctgcc ggcggttggg cagaacgtga cgcagttgcc
     1381 gcattcgttg cagtagtcgg tcagcaccgc ggtttgcacg ccttgcagca ccacaaaacg
     1441 ctgcggtttt ccagccacgg cctgaccatt ttggacggtc cacgtggcca gatcggcgga
     1501 gaacggccgg caatcatagg tcaggaaggc ttgattggga cacacgctgg tgcagatgga
     1561 gcagaaggtg tcgcaccgca agcagcgggc ggcttcggct tgcgccactt ccggcgtcat
     1621 ggtttggatg acctcggcaa atccgttgcg ctgcgccaac ggcaattccg gaatctcgac
     1681 gcgcgggtag cgggtggctt ggcgtttcaa cgtggctacc atgtccagcc cgcccgtgca
     1741 gcgcataggt tccggggtgg cggggacctg cgcgatgagg cggaggattt cggcggcaat
     1801 tttcttgcca tcgccttcag ccttcacgag cgagagcggg ccatcgttga tggcatcgcc
     1861 gcccgcgaag acgttgggta cggaagtcgc gccggtttcc agatcggcca caatatagcc
     1921 cttgcggttg gtggcgatgc cgctctcttt gatgaaatcc aggaacggct gctggccgat
     1981 ggccgcaatc aggatatcca gctcgatggt gaagtccgcg ttgggcactt cgacagggcg
     2041 gcgccggccg gaggcatccg gttcgccgag ttgcatggtg gcgcaagcca acccgcgcac
     2101 tttgccatcc ttcaccacca ccgacttggg cgcggcgagt tcacgaatcg gaatttgctc
     2161 gtgcagcagg ctcatcagct cttcgtgatg cgccggcatt tcttcgcgag tgcggcgata
     2221 aatgacggtg acatccccac cgagccgctt ggcgacgcgc gcgcaatcca tggcgacatc
     2281 gccgccaccg atgaccccca cccgcccttt gagcgacttg agttcgccat cccacacgcg
     2341 gcgcagcatg gtgatgccgt caagcacacc ttcggcgtct tggccgggga tgcccagttc
     2401 cacgccgcgc atcgcgccgg cggcgacgac gatataatcg aagccctgct ggcgcagatc
     2461 gttcagcgag aagtcgcggc cggcggtttg gccgaagcgg aacttaacgc cagcggcttc
     2521 gaggcgcttg acgtcctgct ccaccaccgg cggcatcagg cgatacgttg gcagcgtcat
     2581 ggtggccatg ccgcccgctt gcggacgcga atcaaagatc gtgacatcca ccccgccctc
     2641 gcggaggaaa cccgctgtgg aaatcccgca cgggccggcg ccaatgatcg ccactttgcc
     2701 ggggcgcggc gcgcccacct tgatggcggg atcgcggccg gtctccatga tgaatcgctt
     2761 catttcgcgg atggccagcg gcagatcgta gtgcacgcgg gagcatggcg tttcacaaat
     2821 gtggtcgcag gcgcgcccga gggccgcgcc catcatgttg tcgcgccgca cgatggccgc
     2881 ggcttcgtcg aattgcccat ccttgaccgc ctgcatatag gcgggcacat cctggctgat
     2941 ggggcaaata tccacgcatg gcgccttgac gcaatcgaag agccccagcg ggcgcgaggt
     3001 cttggtgact tgccgcacgt aagtatcgcg ctgatacagc gggttttggc gtacggtggc
     3061 cgcgtaggcc accagatttg ccagggcggc ggccttgaca tcgcccgcct tgccgccggc
     3121 cttggccaga atgaacgcat tgaggtcccc tgcaccggcc ttttgcatgg ccacggaagt
     3181 ttccgaaata tattgcaagg tgcgcaggta gccgccaggg cgcagcaagt cggagctggt
     3241 ggtgatggtg gtcatgccgc aggcgagcag atcggccacg ttccagcaat ccgcgccgcc
     3301 ggcatacgac atcatcaaat cgccacggaa ttcttgcgcc aacttggccg ccagattgac
     3361 ggtgatggcc tgcagcgggc gaccggacag gtacatcatc ttttctttct ggtcgaacac
     3421 gcggcggtgg ttgattacct cgagggtgtt ggacagcttg acgccgaact gcacaccctt
     3481 cttggcggcg cgcgcgcgca gatcgttcag aatgttcaat gcgtcgggat atttgagatc
     3541 gtggccgaac gcctcatccg gcacggtgac ctgccggaag ccgagatcgc ggttcaagat
     3601 accgcgcaaa tcttccgggc cgagcagggt cgggttcagc ttgacgttgg tatgcagccc
     3661 gcgctcctcg atgagatacg cggcaatccg gccgatttca tccggcgggc acccgtgcat
     3721 agtggacagc gtgacgttgt cggacaggcg gctgggaatc gccagctcct tgaccgctgg
     3781 atacaccttg gccacggcgg ccaccaccgt ctccaactcc gcgcccgcgt tcgccatgcg
     3841 gttgagaaaa ctctgcacat tgggcttgag gatgccttcg aggttgtagc ccacgctcat
     3901 gttgaagatc atggccggcg cgcccgcgcg gccgagcttg tgctgcaagg cgtggatcaa
     3961 cacccaggcc atcaggtatt cggtgaacga ctggtcaatc ttgagttcct gcgaccactc
     4021 gacgttgtag ccttcgtcct ggatatcaat gcagggcttg gacactgcca attcgtcgag
     4081 ggtctgaatg gtcttcagct cgatgaaccg cgaacccgtg agccaagccg tgatgatgtt
     4141 ttgcgccatt tgcgagtgcg ggccagcggc cacgccaaac ggggtttcca acggctggcc
     4201 atacaacgca gtccggaacg gatccgccgc gccgggctgg aaaaacaact cgcgcggaat
     4261 accaaaaatg gaattgcgct tttccagttc agtgaaaacc caagcggcga cttggtcgta
     4321 ggtcagcggt tgaaaatgat cagacatggt tgtactcctc gcactgctag catttgacat
     4381 cgggccggaa aattccggac gtgtgaaggg tgattcttac acaccccgcg cgcgatgagc
     4441 aagcgcccag ccttggtgcg cggcactttc tgcgaaaccg tttcaccaga ggccaacttc
     4501 gctccccccc ccaacatttg cgagcgtcag cgcccaccat cgcgccacta ccggcgcgta
     4561 agagggaacg ccttgttcca aaccttcatc ctttagggct tcgtggggat ttcattcgtg
     4621 tgattaaatt ggggatcagt gattattttg ctttctattt gaatcggatc atttggcccc
     4681 aacagttcgc aggtggattc cagtttgtag tcccagacca ctttccttgc ctttcgaaag
     4741 atgtcaaagc tgtccggccg aaccttaaac caaatgtatt gagtatcggg gtcgagttca
     4801 gtcagttgtg agccgaacca cttttcatcc gtttcctcac gaggagaatc gaatgagtac
     4861 ccttgggttt ccgggtcggt tgcttgaggc tgctcaagcc gaatgaaaaa tagttgattg
     4921 tgtcggcatt cgaaaacaac cggccgtttc tcctcggtat tcgcctcaat aggggtggga
     4981 gttataaccg tggcgttttt ggccaaatag tttgaaacca tttgaggagc acgctttcgc
     5041 atcacgttcc agagatcatc aaccaatgga ctatttattg ggtaaggaaa gatgtaggga
     5101 tttcctccgt tggcttcaaa ccgatcaacc aatgtctcaa agggatttcc cggtatctga
     5161 agttcttcgc gcgtaacaac aattttgtcg ttgtcaaaaa tgatgcgatc cttttctacg
     5221 gttacaggat acatcctaat gtgttcaagg gggtccggtt tgggcggatc ggcagtaccc
     5281 atttcataga ttcccatcag ctctattggg gagaattctc gtccttcctc ccatacatcc
     5341 acgccaatat ccacatcgta ttttcgaatg gtttggcgaa gctggtgttg gagtatttca
     5401 ctcccgggcc gcaggatgag cagtacatag cggctattgc gtacgcttgc cacttgttcg
     5461 agtaagcact caaaattgtt ttggggaacg gtcaactcag attccgcgat ttcaatcttt
     5521 tctggcatca cgatgatcct gtctggatgg acatcaatat atacgggatc tttccgcatg
     5581 ttgtatgggc gggcgcctgc gaacgcgatg gcgggaatca aggcaagcca gagccattta
     5641 gtcatgtggt gccgctgtgt agagtctcgc acagtcattt tatcgtatct cttattctga
     5701 acagtgaatc agtgccggcg gtgccaggcg cgcggttctt tcaagaccac ggtggcattg
     5761 gtgccgggcg ggatatccaa gacgcgctgc tgacgaatat attccagcac catgtcgcgc
     5821 accacgacat cgctggtttc cagccgcgcg ttgggcattg cggccatctt ggcaatcttg
     5881 gggaaccgcc cgccaccccc ggccaagtga taactgttca gcgcgacttt tagccggcgc
     5941 ttgccgttga tggcactgcc atccatggcg cgcaggttgc gaatccggtg gccgtgggga
     6001 gccttgggat acaggtcata actcaagccc cacgcgccgg gataacgctc ggtgccgaga
     6061 tattccgtgg cttcctccat cagcgcctgg atttcgccga gggtcagcca gaggcagccc
     6121 gcggtatttt catagggcac gatgcgccag acatccgcca cgcgaatatc gccggcggga
     6181 atgccggttt ccaccagcgg accgtgcagc accacttccg cgccggtatg cgcggcgatg
     6241 gccgcgctca acagttgctg caccgggcac aagccgggca gcgcggcgga atagttgatg
     6301 tcccgcttgg tggtccccac ccgggtttcg agccatttgg tggctttggc gaggtcgcgc
     6361 tcgaccagtt tggccagagc cggatcttcc ggcacatcgt catcaatggg cacaaagcgg
     6421 aattgtttgt ccaccaagcg gttttccacg gtgtcgtagg caaagtctac gcgcatcacg
     6481 ccggcggcgc catagccggc ctgtgcgtag ctcacgttac ccacgcggat gccggtcagc
     6541 acatagtgca gatgcccgcc cagcacggca tcaaactcgc cgaagcgccg cgcgatggtg
     6601 ttgatttcgt tggcggcatc gtcggaactc aacaaccctt gatgcaccag cagcaccatg
     6661 atgtgcggct gctgggcgcg aacaagcggc agggtgcgct ccaacgcgcg tcgcgaatca
     6721 gtgaaggaaa tatcctgctg cgcgaggccg cgcgtccatt gcgggatatt gggcgtggtc
     6781 agcccgacca cggccacgcg gatgccatcc acttccttga tgatgaacgg cagcacgcgc
     6841 tgcaacgcca gcggcgcacc ggacccgacg cgcatgttgg ccgccagcgg caccgcccgc
     6901 atttggccca gaaacgccgc caacgtctcc acgccccagt cgaattcatg gttgccgatg
     6961 gcgaaggcgt cgtagcccaa ttcgttcatc accgtcgcca tgacgcggcc ctgcgtcagg
     7021 aaactggccg ccgatccctg aaaaatatcg ccgcaatcca gcagcagcgt gtgcggatta
     7081 tccgcctgcg cgcggcgaat caaggtggcg cattgcagca gcgagcccgc accacggccc
     7141 gcttcagcga tggtttcctg ccggagcgtg ccgtgcaaat ccgtggtgtt cagaatggtc
     7201 aggttgacca cccgcgcgcc ggcgtccgtc gccgccagcc agatggccat gaaagccacc
     7261 ccccagcggc cagccatctt gcagcagatg gttgaccgcg tgcggttcat cccgcctctt
     7321 ccttgggcag aggctgggta tccagaatgt tttcctggcc cgtcaccgtt ttttcgctga
     7381 tgacataatg cttggcgcca cgctggcgcg gggcttcata catggcatcc agcatgatgg
     7441 attccagcag cgaccgcaac ccgcgcgcgc cggtgttgcg gcgcacggct tcgcgggcca
     7501 attcccgcaa tgcagccggc atgaagtcca gtttcacgcc ctccatcgcc agcaactttt
     7561 ggtactggcg ggtcatggca tttttgggct cggtgagaat gcgcaccaaa tcgtcttcgg
     7621 tcagatcgtc cagtgtggcc accaccggca ggcggccgac aaattcggga atcatcccga
     7681 atttgaccag gtcctccggc tcgaccgaat ggacatcatg ccgcgccttg aggcgcttgc
     7741 gctgttcctt ctcgctgtcg aatccgatca cccctttgcc cacgcggcgc ttgatgatgt
     7801 catccagccc aacaaaggcg ccaccgcaaa tgaacaggat attttccgta ttgatcggaa
     7861 tggtttcctg ctgcgggtgc ttgcggccgc cctgcggcgg cacgttggaa atagtgcctt
     7921 cgagaatctt gagcagcccc tgctgcacgc cctcgcccga cacatcgcgc gtgatggaca
     7981 cgttttcggt gcggcgggcg attttgtcaa tttcgtcaat atagacgatg cccacttccg
     8041 cacgcgcaac atcgccatcc gccgcgcgga tcagccgcag cagtacgttt tcgacatcgt
     8101 cgccaacata gccggcttcg gtgatggtgg tcgcatccga aatgctgaac ggcacatcca
     8161 aaatgcgggc cagagtccgc gccagcaggg tcttgccgct gccggtcggc ccgatcatca
     8221 ggatgttgct cttgtcaatt tcaacatcgt cgtcggccgt ttttcccgcg aggcggaact
     8281 tgacccgctt gtagtgattg tgcacagcca cggccaacac ttttttgacg cgctcctgcc
     8341 ccaccacata ttcatcgagc ttggccttga tttcgtgcgg cttgggcacc ttgaggcggg
     8401 cggcatccgg ccgcgggccg gcggggccct ggttgcgcgc caacaccgcg ttgcacacat
     8461 caatgcaatc gccgcagatg ctcatgtcgt ccgggccggt gaccatggat ttgaccgact
     8521 tgcgctcgcg gccgcaaaac acgcagaatt cgctgcgcgg aggtagacgt ttgccggcca
     8581 tgcttatgcc ttggcggggg ctgcgcccag cacgtcatcc accaggccgt atttgacggc
     8641 ttcggcggca gacatgaaga aatcgcggtc ggtgtcgcgg gcgatatcct tgacggactt
     8701 gcccgtatgc ttggccagca ggccgttgag ataatccttg aggcgcagga tttcctgggc
     8761 ctggatgtgg atgtcgctgg cggtgccttg cgtgccgccc cacggctggt gcaccatgat
     8821 gcgcgcgccg ggcagcgcgt gccgcttgcc cttggtgccg gcagtcagca gcacggcagc
     8881 catgctggct gcctgcccga ggcagtaggt cacgacgtcg cacttcaaaa actgcatggt
     8941 gtcataaatt gccagcccgg cagtcacgga gccacccggc gaattgatat atacgctgac
     9001 atccttggtg gcgtcctcgc tttggaggaa caggagctgc gccaccacca aattggccag
     9061 cgcgtcggaa atttccgaac cgatgaaaat gatccgctcc ttgagcaggc gcgagtaaat
     9121 gtcataggac cgctcgccgc gcccggtttg ctccacaaca atcgggatca gataagcatt
     9181 ctcattcatg ggtatcctcc tgcttttggg cggcttgttc cgcacccggg ccggtcagtt
     9241 tggcgtggct gagcacggcg tccatcgcct ttccgttgcg caggccggtt cggatgtcac
     9301 gccgcaactt ggcttcgttc agcttccgcg tgcggatgaa atccttgacc gacactccgt
     9361 cccgttgggc ctgccgcagc aaggcgctgt ccacttcctg atccgtcacc acgaggttct
     9421 ccgcgtcggc gatgcgcttc agcagataac gcaacttgag ctgggtccgc gccgcttcct
     9481 tggcggatgc ggaaattttg tcgaggtttt cgcgaatctc ctgctccttg acgccgcgct
     9541 gcaaattttc atccacaatg gaatagacca gattgttgtg aacttcggac tcttccgact
     9601 ccggcaggtc gaggttctcc accgtcttca acagatgtgc ttccacctgc tcccggcgac
     9661 gccgctgctc ctggatctcg gcctgctggc gcagcagttg gcggaacata tcccgcacat
     9721 cctcttccga ttttgcgccg atcctctgca ggaaggcttc gtccagcacc ggcatcttgc
     9781 gccggcgaat cttcttcacg gtggtcttga atacgccctt tttgccgcgc acttccgccg
     9841 gttgggcatc ctgttcaaac tggacggtga tgtcgcgcgt atcgcccacc ttggcgccaa
     9901 tcaattgcgt gccgaattcc ggcaggaagg agtactcctg attagccacc acccagcgat
     9961 ccgtagcttc cagcaaatcc ttcaattcct tggacatttc cgccggcact tcgccatcga
    10021 gcgtgctggt gtaatccacc gccacgatgt cttcgagctc caccgggagc ggttcggtca
    10081 catcttcata ttggccgaga cttttcaggt agtcgttcag cgcttgcgtc actgcctcgt
    10141 cggcgatttc gatcttcttg gcgtccaccg cgagcccttt atattccggc agctcgaatt
    10201 ccggcgccac atccaccacc agggaaaatg aaaacggttg gccgctggcc aaggtggatt
    10261 gctgcaagtc atactccgca acggtttgca acttgtgctc cttcatggcc tgctgaaaac
    10321 ccgtcgccag caggtggtct ttgaggacgc tcaggatttg cttgtcgtag gcgcgccgga
    10381 tcatggcttc gggagctttg ccgggacgga accctttgac cgtgccatgg cgcaggtaat
    10441 atttcaggga agaaccatac tcgttgtcca cttcttcggc ggaaaactcc acggaaatct
    10501 ttttccggca agggccggta ttttcgacat taaccttcat gggggaaaag agtcctttcg
    10561 ctacaaaacg gaataaacag cccgcaacca tacccgcgcc cccctacccc gtcaagcttc
    10621 gcaccgggcc gaatccggcc ggttttgcgc cctcggcggc tacccggaaa ggcgggtggc
    10681 caactcgtcc cactcggcgg ttagttcagt gatttgtcgc gtgatgtcct gcaaccgacg
    10741 cccggcctcc tggcggtctt cgggcgaggt ttccgccgcg gaaagcaggg ctagcaactc
    10801 gggctgcccg gactccaacg cggcaatttt ctcttccacc acggcgaggc ggcgctccag
    10861 tttgcgctgc tcggctttct gggccttgcg tgcgcgttgg gcttggattt gtgcttcctt
    10921 ggctggggtt tcggctgatg ccgccgcctc cgatgccgcc ggagcttcgc ggccgatttt
    10981 ttcgcagtaa taatcatagc cgccggcgaa gcggcgcaca cccccgcccg ctggcgccac
    11041 cgccacaatg ccccccgcca ctgcacggac aaatgcgata tcatgtgaga ccaacaccac
    11101 cgtgcccgtg taagcggcca gtgcctcctg caaggcctcg cggctttcca catcgagatg
    11161 cgtggttggt tcatccagca gcagcaagtt cggcggccga atgaggatcc gcgcgaacgc
    11221 cagccgaatg cgctcaccgc cggacagtac ctccacgcgc ttttccacgg cctcgccgga
    11281 aaagccaaag cagcccagca gagtgcggat ggaagattcc ggttcttccg gcgcgaccgc
    11341 cttaagcgta tcgaggcagg tgcgggtgcg gtccaaggtt tccgtcaact cctgactctg
    11401 gtatcccggc acaaccaagt ggcccagcgt gcaggagcct tcgccgggcg gcagttggcc
    11461 cgccatcagg cgcagcagcg tagttttacc ggcgccgttg ggtcccacca cggccagctt
    11521 ttcgccgcgc tgcaccgcca aatccacacc gcggaaaatc cattgccccg gctcataggc
    11581 aaagcccacg ccactgcagc gcaatacctc ctgcccactg cgcggcggcg gcgccaagcg
    11641 gaactcgcga cgcccgcgcg caatgtccgc gacggtcgtg cgctccatct tctccagcat
    11701 tttgattttg ctttgcacgc gcgtggcgag ggtgttcttg gcccgaaagc gttccacaaa
    11761 ccgttcaatt tgctcccgct tgcggtcttc gttggcttgc cgggccaggg ccagttcgcg
    11821 acgtttttca cgctccacgg catagtagga atagcgcccg gggtagcggg tcacttgacc
    11881 gccggccacc tcgacggtga tttcgcaaag gctttcgagc aaatagcggt cgtgcgagat
    11941 catggccagc gtaccgcgga agcgctccaa tcgccggcgc aaccattcca cggccggaac
    12001 atcaagatag ttgctgggtt cgtccagcag caataaatcc ggctcggcga gcagggctcg
    12061 cgccaactcg gcgcgcattt gccagccgcc ggaaaactcg ccgagggggc gttgcagttc
    12121 ctcggcgtgg aaacccaggc cggccaatgt cgcggccgtg cgcgccggca catcgtagcc
    12181 gccttgtagc tcaaactggg tttgcaactc gcccagttgg cgcagcagga tgcgtgcttc
    12241 ggggtcttcg ccggtggcga gacgcgcctc gatgtgctgc acctcgtgac ggatggttgc
    12301 gctgctcggc gcggcttgct ccacataggc ctgcacgggg atttgctccc cgccagagcg
    12361 cagttgctgg cgcaggtagc caatgcggat atccttggcc aatgtaatgc gtccggcatc
    12421 ggcctcgcac tcgcccacca tcaggtcaaa cacggtggac ttgcccgcgc cattgggccc
    12481 cacgaggccc acgcgctcgc ctgccagcaa ccggaaatcc acccggtcca gaatgatttg
    12541 cggcccgtag cgcttgctga cttgccggaa ctcaatcatg ggcgcaggcg catggggaaa
    12601 atggaaagcg aaagtgtgaa ccgcaacgcg ccgcgtttct caactggccc ggcgagtccg
    12661 ccacgggtca ggatttggcc gccagcctga tgacctgctg ctcgctggcc accagcagat
    12721 tgtatagctc tttggggtcg ttggtttcgc gcaattccaa gagcaggctg gtgtggtggc
    12781 acatcatgca aagacgcgcc agcacgtgca ggtggatgcg gtcattctgg cagcacgtca
    12841 ggaagaaaat atccgtggtg gtgccgtcgg gcgagccgaa tggcacttgg gcgacggttt
    12901 tgcccaacac cacaaacgag tcttcgaacc ggtagggctc gtggttgcgc gggtgcaaca
    12961 aggccaaccc gccggaaagc gcggtggagc agagtttttc gcgctcgatc aggctttcca
    13021 gcaattcgtc acgataacac agcaggccgg tggcgtcggc caggtccacc atttcgcgga
    13081 tcaccgaggc cttggagcgg caggtcattt ccggccggat ccattccggc ttcatcaatg
    13141 ccggaatgat ggcggcttcc gccgacaggt cgtgatattt ggccgtggtg cggcggtgga
    13201 aatcttcgat cttgtccgtt gacgagccca gcaattgctg cgaggcccat tcatccacct
    13261 gattatgccg aaagaccagc cgcccgccct gccgctcgca ggggatttcc ccgcggcgcg
    13321 ccagctcttc caggcgctgg gccgacagat gcaaatattc cgcggcttcg gccaaattga
    13381 acgtccggaa gggcatcgcc agtcaccatg ccttcctaaa tacccaagag ccccgtggca
    13441 aacgcgacca acagcagcgc gcaaaccccc agcaagccgg ccgtgaacaa tggaaatggg
    13501 ctgccatcgt tttgctgacg caggcgcttg gcaaaaaaag ccgcgccacc aacagccatc
    13561 gtcagcagca gcatgacgcc aaaaaacaac tggtttttcc agaccgtggc ggccggcccc
    13621 gcgccgggcg agccggagcc ctgcatggcg gctttgccca ccagcggcag gatcatgcac
    13681 tggaacagga acaacaaccc gaatagtgtc gagagggtct cgcccatggc gggcgtggac
    13741 aggatatccc gccacgaggc cggcggcggt ggctccgact tcttcggttg ttgtgcagtc
    13801 tttttcatca ggcgcgcatt ttgcacattt tcgggcgcgc agttcaagca aaaggcgaaa
    13861 attgaaatcg agtcattgcc ccggaccgaa taagatgcta gcgtgaatag ttacgtcaac
    13921 agcaggtaaa acccgacatg agcaatcccc aaccgatcat ccgcaaagcg cgcttcaacg
    13981 acggcccggc cattttcaag cgcatcaagg ctcaccccaa cgagctggtg cctcggccca
    14041 tcagcgacat catgctcaac attgaccgtt ttctggtggc ggaggccaac ggcgagattg
    14101 tcggcactgc cgcgtggagc gtgctgccgg agctggacac caccaaaaat ccgtccgtcg
    14161 aaatccaatc ggtttcggtg cgagaagatt accagaaggc cgggctgggt cgccggctgg
    14221 tggaggccgc cctggcgcgc gtggcggaat tgaagccgga ccaagtcatt gtgctgacct
    14281 tcaccccgcc gttctttgcc aagctgggct tcgtgccggt atccaaggaa aagctgatgt
    14341 ataaactgta caagggctgc atcaactgca ccaaatacga ctcgccctac acctgccccg
    14401 aagaggccat ggctctggat ttccggaact ggaaaggcaa cgcttcgtga tggcggggcg
    14461 ctggcgcatc ctcattgcgg cagccctgct ggcggcaggc gtgcccgcgg ccgcctgggc
    14521 gtgggccggc gtgcagcaca tccaaatcaa caagctcgcc gggcgcaatg ttcccgacga
    14581 aatgcaggat ttccgcaact tttcccggcc gatggcgctg ccggggattt ttcccgactt
    14641 gtggaaagag gccgatcccg atgaatcacc gcgccactat tttgagccgg accgtctgcc
    14701 ggcgggcttc gatttgaagc agctcagccc ggacctgccc accgccctcg gccaaatgaa
    14761 catcgcgccg gagatcattg g
//
LOCUS       DAOPNP010000057        14404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4921_length_14404_cov_8.673915, whole genome shotgun sequence.
ACCESSION   DAOPNP010000057 DAOPNP010000000
VERSION     DAOPNP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_14404_cov_8.673915"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..416
                     /locus_tag="PKI64_06570"
     CDS             123..416
                     /locus_tag="PKI64_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00711.1"
                     /translation="MNPLVKRVVLLLNTVGFVAYLVWLSTFSTGETLRSQDGILFYLP
                     CVPFLFVYMLLMPQKPAAKAKPWWQSDEDFAREQREKEAAANPPPPPSPPPGT"
     gene            426..1307
                     /locus_tag="PKI64_06575"
     CDS             426..1307
                     /locus_tag="PKI64_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE00712.1"
                     /translation="MASPERKITLVSIAVNIVLTVIKLAAGVLGRSQALLADAVHSLS
                     DFATDIAVLIGLRFSSKPVDEDHAYGHGKYETIVAAIIGLALAYVAVQIATSAIGRIF
                     GALHGKELPRPATYAFWVALASFGVKEWLFHATMRVARATGSSALVANAWHHRSDAFS
                     ALASSVGVGAAAFLGADWLILDPIAALFVSMFLLRVAWQIIRTQIGFLTDQSLAPEVC
                     EEILALARATPGIMEPHNLRTRMVGPICVIDLHIQMRADLSLAAAHDIADALEHTLRQ
                     RFGKETIANLHLEPYAG"
     gene            1477..1740
                     /locus_tag="PKI64_06580"
     CDS             1477..1740
                     /locus_tag="PKI64_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00713.1"
                     /translation="MEQGAAKIAKLLLVAILLGVSVTAFNPSYRQAFLALARGTPQEN
                     PIWKSNADYYPDIVLPGMPPWKPVLPVDDRNIPAAAREAAAQP"
     gene            1756..5130
                     /locus_tag="PKI64_06585"
     CDS             1756..5130
                     /locus_tag="PKI64_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOE00714.1"
                     /translation="MTRNKLISFCYFFIVGIGLAMLYPAQADALTLLWGLTGLAALAT
                     IAAFVYVYRHPRNVEEELAFGAPPPNRQQITLWVLMLLAAVIFGYTRYIAFIQAPDTV
                     VGTLAVTSSGQTWKDGRPISQTSFLKVVNLTPVKEDIHLRLVGRLEALEPNRDTAGVP
                     IMDADGNWTFTQYNLPQTSDEIIIPAGTPARSVVLVEQAFTHLDRVELVQPPAQPVKL
                     AILQPENTVALFARSGHTVVPISVLGRINSDPWVYSFKTILSVKPDFIQYQPDGPFFP
                     VDRQNIRITVDPETPGYERLACSAAYGYDVYVTGELVSAPHSSNKAGFDQANYLRSYN
                     IGGQMRLRVPREGPAPIAIVTPAGAAAPRQGNGLVEFSLYLRDEIVRVIKQTTPQPNS
                     AFHGALTLGLRYGMQSTVSMANDMYKTSPVPPLIPLGKHTDRLIADEFRYSGINHVLA
                     VSGLHVTIITVLFMGIFILLKVSKKVYVPMIIFALVVFAVITGARPSTLRAVIMNSLF
                     LLTWGYLGESVRSSALLGVPVAALMILLQNPAMCVDPSFTLSFMAILSLALMTQPFFD
                     LFKRLKGNNFAALVILLGVLTWAFAAHWFLVTTLRFWLFLLVFGIALFALARFLTKIG
                     FTPLRDYGLANIPPAIAGFFAAQFGIQIGMMIPLSPYYFMRWPVAGAYANLIAIPLVG
                     VVLQLSMLAGLLGLIPGIGIYLALLLSAANWVFSIGFLLIGHYFSRWFSYPFMIKPTV
                     FDLGVYFALCAVFVWWRPLWFRIVRPGWRNAPRSRRVIFVALLLLLGGGVAAKRHSEM
                     ERMRPAGKLEISVLSLGYASSILLDTPTRETVLIDTGMVVTSPATRVDAERTILPQLY
                     ARKMTRLDELIVTTPNPEHSAGIATILTGIPVDHFTYAPGVAPLLREEPVAGLLAERS
                     PARLKHRLSGTTLAPRAVAPGDVIYRAEYAGKPILIEAIGPADPAGENPLSLRIEYGA
                     YAMLVLSDLTLAQQKAFLAGTPPEKLKAQVVVAPHHGTAGIEDITLGLPDDMAQRLAD
                     STGELLKATGAEAVIFEFGNPRPVEPLRYKEARKIASLARRAAEDALPDAINVATDTD
                     GAIHISSDGITYQLATQLSETGSEVDEPTMLEIGGF"
     gene            5498..5824
                     /locus_tag="PKI64_06590"
     CDS             5498..5824
                     /locus_tag="PKI64_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00715.1"
                     /translation="MTQQTARWQGQSGRTYDYEVHLIGTTFAEKPGNYIFAAISDGRW
                     KACYIGQTENLHDRLANHEKEPCAKRYGASHVHAHVTTGGEAVRKAEEEDLIRLHKPP
                     CNDQLK"
     gene            5895..6998
                     /locus_tag="PKI64_06595"
     CDS             5895..6998
                     /locus_tag="PKI64_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE00716.1"
                     /translation="MNAMVPNNGLALLTTRTMGETAGLRRLAEETRVAIALLNYQVGQ
                     MPSPNLLLDLLPYQEARGSSQIENIVTTNDELYKGLAFGEYTETAKEVVRYKTALFAG
                     MDILKARGVLSVGDIETIHAQLAANAPGLRTNLPQFESSLTRVARVKSGEREVIYTPP
                     HGKPLLQRLLLDMLEYVYNDEMYPAHPLIKIALAHMQFERIHPFHDGNGRTGRILNIL
                     FLCQKGYLALPVLYASGEILRRREEYYAAFTVPEEEQGAARFAEFMLGIFQDTARRTL
                     KIVNRIGKLTADHTSDQFLNQLRGRKSELQKVLATVFSKPYVRISDLNELHRETASRH
                     LRQLVEKGVLREEKSGRDKVFLNEVLLGIFHDE"
     gene            7250..8980
                     /locus_tag="PKI64_06600"
     CDS             7250..8980
                     /locus_tag="PKI64_06600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOE00717.1"
                     /translation="MNHADIQTKAKLVTDIANKLRGPYTPERYGNVIIPMAILRRFDC
                     VLADKNAAIKPVLAKMERLKADDEHKEQAVKKACGVPFFNAKCVTFEDLVGDSDNLGA
                     NFLEFIGAYSKNIAEIVKGLKFREEVEFMIKKGLLFEVVKAFAAVDFHPETTDPLSMG
                     YVFEEIIRTYKANAEAGDHYTPREVIELCVDLMMAEGAEELKKPGKIIRVYDGCCGTG
                     GMLTVAKRKLKELLGTDEGIRLFGQDSNPESYAICRAEMLMLGEDPRNIHEGNTLTDD
                     KLKGEKFDLTITNPPFGYEWKTDKALVEQEFKAKYGSRFMAGLPRISDGQLLFLQNLI
                     AKTEKNGRIAIILNGSPLFSGNAGSGESEVRRYVLENDLVEAIVALPDQLFYNTGIST
                     YVWILSKNKAKKRHRKVQLIDATGCWTEQKPKSLGNKRKEITPEHRAEIVKLYRGFKE
                     GDRVKILETEDFGYHKVTVERPLRDEQGNIVLKKGKKQADSALRDTENVPLKEKIADY
                     IAREVLPYAPDAWVDDSKTKIGYEIPFTRYFYRYAPPRPSRDILQEVVAIEDDIRDTL
                     ASLLGRNGNP"
     gene            8977..10278
                     /locus_tag="PKI64_06605"
     CDS             8977..10278
                     /locus_tag="PKI64_06605"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOE00718.1"
                     /translation="MKPRKMKDSGIPWIGLIPEGWEARKIKTIFQVLGGATPASGNVD
                     YWDGDIPWVTPADMSDDKIYLKDSKRKITREGLESCAAELVPVESIIVSNRAPIGKVA
                     LAGVPLCTNQGCKSLRASESVCSKYFYYYLRTQTETLNMFGQGTTFLELSSRSLNDFL
                     VPYPAENEQSRIAAYLDEKCGEIDRVIAAKEQQNKLLRDQRTAIISEAVTKGLNPNAK
                     FKDSGFEWLGEIPVDWCHLPLRYAVVSITGGATPQKENPLFWDGDIPWASSKDLKTNK
                     IYKTEDSITKEGLERCSVSLIDAGRLILCARSGILRHTLPACVNMVPLTINQDLKALE
                     FRKEINIYYVQYYFLGLNREILTFSQKEGATVESLNMELLMKLPIFFPRMPEQEKIVS
                     YLDSRCAEIDQVIAANEKMVAKLKEYRSSLIWEAVTGKTSL"
     gene            10420..11028
                     /locus_tag="PKI64_06610"
     CDS             10420..11028
                     /locus_tag="PKI64_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00719.1"
                     /translation="MYQWRKMTVEEREEALRERRQRRLPHHSPPHFAGDAPRLYHLTA
                     ACYEHRPIVGLSPNRMAEFEAELVKTLRAEGRELAVWCVLPNHWHALVRTGDLRGTTK
                     AVGRLHGRMSHAWNGEADCRGRTCWHRCADRAMRSDAHVFATINYIHHNPVHHGYVEQ
                     WTQWPFSSAADYLDAIGQEEAKRRWKQYPLLDYGKGWDEPEM"
     gene            11256..14189
                     /locus_tag="PKI64_06615"
     CDS             11256..14189
                     /locus_tag="PKI64_06615"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE00720.1"
                     /translation="MAAEDLRENPFRDEICAALVAKGYVEGQKGDYVMDRALDRARLL
                     EFLERTQEPELAKFRAAHGADWQNRLLRLWDETIEHNGLIKTLKGLVEDYPSGAKFHV
                     VDFPRNLAGMGAGSLEKNVFSVRREFIYENKPEPHRVDLAIFLNGIPVAMIELKKRTA
                     GQSAGVEGMRQYRNTRDPREKVFGFNRRTLFYLVMDEFEAFVSTRLAGPETKFLPFNR
                     GTATGGAGNPIEEGKHPTHHVWDELLERDMLLRIIRDYLFIDEEGKMIFPRYHQLDAV
                     LKLERNVREHGMGGRYLVWHSAGSGKTKTISWLAFRLLNLPGIKTVIVISDRKVIDSQ
                     LAGQALTMDGKTGRVQWAEDSAELAALLNEGGMIVVTTLQKFPLVLEKLQEKTGEQYG
                     IIIDEAHSSTAGKTMSKLSETLAGKSLAEAAELEQAYEEREDGQRQLQELEPRITATK
                     NVSYFAFTATPQTKTMELFGTRNSRGKDCFHKYSMRQAIEEGFILNPIACYTVYSDKL
                     KVRKKDEAEGEFESARTAAAILHYLTSSPEVVERKTEIMLTDFLARRQHWLGGTAKAM
                     ILTASRKHAVCYKLAADRFLEERGCDVKAVVGFTGSIVLDDKTYTEENLNAGLEGRDL
                     RQIIQKDPRARIIVVADKLQTGFDEDRLCVMYIDKHLNGPVKTVQTISRLNRARAGKR
                     TFVMDFVNEARDIKAAFEFYYGGELWLPEENEIDPNVLFQKRNRLLEYGVFTMEEARR
                     AYGFIVAEEKHSPELSALFAELIGRHARLPVTPKENKKKLFLAEASQFVSLFYYVSTV
                     YARWDLEMKRLAAFLDTLLNLLKAGEDQEQIRPEKLAELVKFTTRKVLHEQNLLPESV
                     AQELDSVSTEVRVAEPKVSALDEIIQRINAKYGYEQAKDAIESIVTTLAADAGLAMNV
                     RNSAKSAYEAETMDRLNEICVNGMLSSEGEQERFYAELSKDKEFLQNLAFEVIRRIQQ
                     IPQAV"
     gene            complement(14318..14394)
                     /locus_tag="PKI64_06620"
     tRNA            complement(14318..14394)
                     /locus_tag="PKI64_06620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14358..14360),aa:Pro,seq:ggg)
BASE COUNT         3223 a         4457 c         3997 g         2727 t
ORIGIN      
        1 accgcttctg ggatgatcgc gcggcttaat aaccgacttt tatgtcgtgc acccaggttc
       61 gcgccccgcc cgcccaaaac ccgctgttgc caaagagggc gccgccgcgc taggttgtgc
      121 ggatgaatcc attggtcaag cgcgtggtgc tgctgctcaa taccgtcggg tttgtggcct
      181 acctggtctg gctctccacg ttcagcactg gcgaaacctt gcgctcgcag gacggcatcc
      241 tgttttacct gccctgcgtg ccgtttttgt ttgtgtatat gctgctcatg ccccaaaaac
      301 ccgccgccaa agccaagccc tggtggcaga gcgatgaaga cttcgcccgc gagcagcgcg
      361 aaaaagaagc cgcggctaac ccgccccccc ctccctcccc gccccccggc acctagccgc
      421 ccgccatggc gtcccccgag cgcaaaatca cgttggtttc catcgcggtc aacattgtct
      481 tgaccgtcat caagctggcc gcgggcgtgt tgggccggtc gcaggcgctg ttggcggatg
      541 ccgtccactc gctctccgac tttgccacgg acatcgccgt gctcatcggc ctgcgctttt
      601 cctccaagcc ggtggatgaa gaccacgcct atggccacgg caaatatgaa accatcgtgg
      661 ccgccatcat cggactggcc ctcgcctatg tggccgtgca aatcgccacc tcggccatcg
      721 gccggatttt cggcgcgctg cacggtaaag agcttccgcg cccggccacc tatgcctttt
      781 gggtggcgct ggcctctttt ggcgtcaagg aatggctctt tcacgccacc atgcgggtgg
      841 cgcgcgccac cggcagctcc gcgctggtgg ccaacgcctg gcaccaccgc tccgacgcct
      901 tttccgcgct ggcctcctcg gtcggcgtgg gcgccgccgc ttttctgggc gcggactggc
      961 tcatcctgga ccccatcgcc gcgctgttcg tcagcatgtt cctgctgcgc gtagcctggc
     1021 agatcatccg tactcaaatt ggctttctga ccgaccaaag cctcgcgccg gaagtctgtg
     1081 aggaaatcct ggccctcgcc cgcgccaccc ccggcatcat ggaaccgcac aacctgcgca
     1141 cccgcatggt cggccccatc tgcgtgattg atctgcatat ccagatgcgg gccgatttat
     1201 cgctggcagc cgcccacgac atcgccgatg ccctcgaaca taccctgcgc cagcgcttcg
     1261 gcaaagaaac cattgccaac ctccatctgg aaccgtatgc tggctagtta atttttttga
     1321 cagttgacca tgaaatcagg tcccacgcat gcccacacca gccgcccggc tttacggccg
     1381 gattggcggg cccaattatc ggttgatgcc cccgtcccct cactcccccc gccggcctgc
     1441 cgccggcggc tctccgcatc atagaaagga tctcgtatgg aacaaggcgc tgcaaaaatc
     1501 gccaagctgc tgttggtggc catcctgctg ggggtgtccg tcacggcctt caacccctca
     1561 taccggcaag cctttttggc gctggcccgc ggcacgccgc aggaaaatcc catctggaaa
     1621 tccaacgcgg actattatcc cgacatcgtt ctgcccggca tgcctccctg gaaacctgtg
     1681 ctgcccgtgg acgaccgcaa catccccgcc gccgcccgcg aagcggctgc tcaaccctaa
     1741 tcccccgccc gtgccatgac ccgcaataaa ctcatttcgt tttgttattt cttcatcgtc
     1801 ggcatcggcc tcgccatgct ctaccccgcg caggcggacg ccctcaccct gctgtggggc
     1861 ctgaccggcc tcgccgcgct ggccaccatc gccgcgttcg tctacgtcta ccgccaccca
     1921 cgcaacgtcg aagaagaact cgcctttggc gcccccccgc ccaatcgcca gcaaatcacg
     1981 ctgtgggtgc tcatgctgct ggccgcggtg attttcggct acacccgcta tattgccttc
     2041 attcaggccc ccgacaccgt cgtgggcacg ctggccgtca cctcctccgg acaaacctgg
     2101 aaagatggcc gcccgatctc gcaaacttcc ttcctcaagg tcgtcaacct tacgcccgtc
     2161 aaggaagaca tccatctgcg cctcgtgggt cgtttggaag cgctggaacc caaccgcgat
     2221 accgccggcg tgcccatcat ggatgccgat ggcaactgga ccttcaccca gtataacctg
     2281 ccgcaaacca gcgatgaaat catcatcccc gccggcaccc ccgcccggag cgtggtgctg
     2341 gtggagcaag ccttcaccca cctcgaccgc gtggaattgg tgcagccccc cgcccagccg
     2401 gtcaagctgg ccatcctgca gcccgaaaac accgtggccc tgttcgcccg ctccggccac
     2461 accgtggtgc ccatcagcgt ccttggccgc atcaacagcg acccctgggt ctattccttc
     2521 aagaccatcc tttccgtcaa acctgacttc atccaatacc agcccgacgg cccgttcttc
     2581 ccggtggacc ggcaaaacat ccgcatcacc gtggaccccg aaacgcccgg ctatgagcgc
     2641 ctcgcttgct ccgcggccta tggctacgat gtttacgtca ccggcgaact cgtctccgcc
     2701 ccgcactcct ccaacaaggc cggctttgac caagccaact atctgcgcag ctataacatc
     2761 ggcggccaaa tgcgcctgcg cgtgccgcgc gaaggccccg cgcccatcgc catcgtcacc
     2821 cccgccggcg ccgctgcccc ccggcagggc aacggcctcg tcgaattctc gctctacctg
     2881 cgcgatgaaa tcgtgcgcgt gatcaagcaa accacgcccc agcccaactc cgccttccac
     2941 ggcgccctca cccttggcct gcgctacggc atgcaaagca ccgtctccat ggccaacgac
     3001 atgtataaaa cctcccccgt gccccccctc atccccttgg gcaagcacac cgaccgcctc
     3061 atcgccgacg aattccgcta ctccggcatc aaccacgtcc tcgccgtctc cggcctgcac
     3121 gtcaccatca tcaccgtcct tttcatgggc atcttcattc tgctgaaggt ctccaaaaag
     3181 gtgtacgtgc ccatgatcat cttcgcgctc gtcgtgtttg ccgtcattac cggcgcgcgc
     3241 ccctccaccc tgcgcgccgt catcatgaac tccctgttcc tgctcacctg gggctacctc
     3301 ggcgaaagcg tgcgctcttc cgccctcctc ggcgtgcccg tcgccgccct catgatcctg
     3361 ctgcaaaacc ccgccatgtg cgtggacccc tccttcaccc tctccttcat ggccatcctc
     3421 tccctggccc tgatgaccca gcccttcttc gacctcttca agcgcctcaa aggcaataac
     3481 tttgccgcgc tggtgatcct gctcggcgtg ctcacgtggg ccttcgccgc ccactggttc
     3541 ctggtcacca ccttgcgctt ctggctgttc ctgctggtgt tcggcatcgc cctgttcgcg
     3601 ctggcccgct tcctgaccaa gatcggcttc acccccctgc gcgactacgg cctcgccaac
     3661 atcccccccg ccatcgccgg cttcttcgcc gcccaattcg gcatccagat cggcatgatg
     3721 attcccctct cgccctacta cttcatgcgc tggcccgtcg ccggcgccta cgccaacctc
     3781 atcgccatcc cgctcgtcgg cgtggtgctc cagctctcga tgctcgccgg cctgctcggg
     3841 ctcatccccg gcatcggcat ctacctcgcc ctgctcctca gcgccgccaa ctgggtcttc
     3901 tcgattggct tcctgctcat cggccactac ttctcccgct ggttctccta tcctttcatg
     3961 atcaaaccca ccgtgtttga tctgggcgtc tacttcgccc tctgcgccgt attcgtgtgg
     4021 tggcggccgc tctggttccg catcgtgcgc cccggctggc gcaacgcacc ccgctcccgt
     4081 cgcgtcatct tcgtggccct cctcctgctg ctcggcggcg gcgtcgccgc caagcgccac
     4141 tccgaaatgg aacgcatgcg ccccgccggc aaactggaaa tctccgtcct ctccctcggc
     4201 tacgcctcca gcatcctact cgacaccccc acccgcgaaa ccgtcctcat tgataccggc
     4261 atggtcgtca ccagccccgc cacccgcgtg gacgccgagc gcaccatcct gccccagctc
     4321 tacgcccgca agatgacccg cctcgatgaa ctcatcgtca ccacccccaa ccccgaacac
     4381 agcgccggca tcgccaccat cctcaccggc atcccggtgg accacttcac ctacgccccc
     4441 ggcgtcgccc cgctcctccg cgaggaaccc gtcgccggcc tcctcgccga gcgctccccc
     4501 gcgcgcctga aacaccgcct ctccggcacc accctcgccc cccgcgccgt cgcccccggc
     4561 gatgtcatct accgcgccga atacgccggc aagcccatcc tcatcgaagc catcggcccc
     4621 gccgaccccg ccggcgagaa ccccctctcc ctgcgcatcg aatatggcgc ctacgccatg
     4681 ctcgtcctct ctgacctcac cctcgcccag caaaaagcct tcctcgccgg caccccgccg
     4741 gaaaaactca aagcccaagt cgtcgtcgcc ccgcaccacg gcaccgccgg catcgaggac
     4801 atcaccctcg gcctccccga cgacatggcc caacgcctcg ccgactccac cggcgaactc
     4861 ctgaaagcca ccggcgccga agccgtcatc tttgaatttg gcaacccccg ccccgtcgaa
     4921 cccctgcgct acaaggaagc ccgcaaaatc gccagcctcg cccgccgcgc cgccgaagat
     4981 gccctccccg acgccatcaa cgtcgccacc gataccgacg gcgccattca catcagcagc
     5041 gatggcatca cctatcagct cgccacccag cttagcgaaa ccggcagcga agtggacgaa
     5101 cccaccatgc tcgaaatcgg cggcttctaa cccggatcat tcacgccata agtccgccac
     5161 ggatgcccgg ccgttggcga cgaagccgga ttggcatact gcgagcctaa aagaatgatg
     5221 cagaagacgt attgagatca ttgtataaaa taatttctat atgtcggatt atattattga
     5281 ttacgacata taaaatatat ttgtcgtatt tttatattga catttggtcg aaagtatgat
     5341 tgacttgcgg gcgacaaaaa aggcggagaa ggttgtgtac accaactccg cccgaggtgt
     5401 ggaaccagtg gcccacatca ctcattgaag acaggggcaa ggtatgcctc gggtgaaggt
     5461 ttgtcaatag atttgccgct gggaaagaga aaaaaacatg actcagcaaa cagcacgttg
     5521 gcagggccag tctggaagaa cctacgatta tgaggttcat ctgattggaa caacctttgc
     5581 cgaaaagcca ggcaactaca tcttcgccgc catttcagac ggcaggtgga aagcctgcta
     5641 cattggacaa acggagaatc tccatgaccg tttggccaat catgagaagg agccatgcgc
     5701 aaagcgatat ggtgcctctc atgtccacgc ccatgtgaca acgggcggag aagcagtgcg
     5761 aaaggcggaa gaagaagacc ttattcgact tcataagccg ccctgtaacg accagctcaa
     5821 gtagcgtggt caacggtggc cggccaagtg ttatgattgg ccggccaccc ttaaagattt
     5881 tgaagggaaa aaagatgaat gcgatggtgc ccaacaacgg gctggccttg ctgacgacgc
     5941 ggacgatggg ggaaacggcg ggactgcgtc ggctggcgga agagacccgg gtggcgattg
     6001 cgctgctgaa ttaccaagtg gggcaaatgc cttctccgaa tctgttgctc gacctgctgc
     6061 cctatcagga ggcgcgcggc agcagccaga tcgaaaacat cgtcaccacc aatgacgagt
     6121 tgtacaaagg tctggcgttt ggcgaataca cggaaaccgc caaggaggtc gttcgctaca
     6181 agaccgccct gtttgccggc atggacatcc tgaaggcgcg cggcgtgttg tccgtggggg
     6241 atatcgagac catccatgca caactcgccg cgaatgcgcc gggtttgcgc acaaatctgc
     6301 cccagtttga aagcagcctg acgcgggtgg cccgggtgaa gagcggtgaa cgcgaagtga
     6361 tttatacccc tccgcacggc aagccgctcc tgcaacggct gctgctggac atgctggaat
     6421 atgtgtacaa tgacgagatg tatccagccc acccgctcat caaaattgcc ttggcccaca
     6481 tgcagttcga gcgcatccac ccgttccacg acgggaacgg gcgcacgggg cgcattctga
     6541 atatcctgtt tctttgccag aaggggtatc tggccctgcc ggtgctgtac gccagcgggg
     6601 aaatcctgcg gcggcgggag gagtattatg ccgccttcac cgtgccggaa gaggaacagg
     6661 gcgcggcccg gttcgccgaa tttatgctgg ggatattcca agacaccgcg cgacgaacgc
     6721 tcaaaatcgt gaaccggatc ggcaagctga cggccgatca caccagcgac cagttcctga
     6781 accagttgcg gggccgcaag agcgagctac aaaaagtcct ggccacggtt ttcagcaagc
     6841 cctacgtgcg tatttcggac ctgaatgagt tgcaccgcga aaccgcttcg cggcatctgc
     6901 ggcaactggt tgaaaaaggg gtgctgcgcg aagagaaaag cgggcgggat aaggttttcc
     6961 tgaacgaagt gttgcttggg atttttcacg atgaatgaaa gtaaccccat gttcgtagtc
     7021 ccgccttcag gcggaaggat cggaaaatgc cgcctaaagg cgggactaca aacggaagag
     7081 aaaaaacgtg ttcgtagttc cgccttcagg cggaaggagc gaaaaccacc gcctaaaggc
     7141 ggggctacaa acggaagcga aaagaacgtg ttcgtagttc cgccttcagg cggaaggttc
     7201 gaaaaaaaca ccgcctaaag gcgggactac taacgtgaaa ggaagaaaca tgaaccacgc
     7261 cgacatccaa acaaaagcga agctggtcac cgacatcgcc aacaagttgc gcgggcccta
     7321 tacgcccgaa cgctacggga acgtgatcat ccccatggcc atcctgcggc ggttcgactg
     7381 cgtgctggcc gacaagaatg ccgccatcaa gccggttctg gcgaaaatgg agcgcttgaa
     7441 agcggacgac gagcacaagg aacaggccgt caaaaaagcc tgcggcgtgc cgtttttcaa
     7501 tgccaaatgc gtgaccttcg aggacctggt gggcgacagc gacaatctgg gcgccaactt
     7561 cctggaattc attggcgcct attccaaaaa catcgcggaa atcgtgaaag gcttgaagtt
     7621 ccgcgaagaa gtcgaattca tgatcaaaaa aggactgctg ttcgaggtgg tcaaggcgtt
     7681 tgcggcggtg gacttccatc cggaaaccac cgacccgctt tcgatgggct atgtgttcga
     7741 ggaaatcatc cgcacctaca aggccaatgc cgaggcgggc gaccactata ccccccgcga
     7801 agtgatcgag ctgtgcgtgg acctgatgat ggccgagggt gccgaggaac tgaagaaacc
     7861 cggcaagatc atccgcgttt atgacggctg ctgcggcacc ggcggcatgc tgacggtggc
     7921 caagcgcaaa ctcaaggaac tgctgggcac cgacgagggc atccgcctgt tcgggcagga
     7981 cagcaacccg gaatcctacg ccatctgccg ggccgaaatg ctcatgctgg gcgaagaccc
     8041 ccgcaacatc cacgaaggca acacgctgac cgacgacaag ctgaagggcg aaaagttcga
     8101 tctgacgatc accaatcccc ctttcggcta cgaatggaag acggacaagg ccttggtgga
     8161 gcaggaattc aaggccaagt acggctcgcg cttcatggcc ggcctgccgc gcatcagcga
     8221 tggccagttg ctgtttctgc aaaacctgat cgccaagacg gaaaaaaacg ggcggatcgc
     8281 catcatcctg aacggctcgc cgctgttttc cggaaacgcc ggcagcgggg agagcgaagt
     8341 ccggcgctac gtgctggaaa acgatttggt cgaggccatc gtggccctgc ccgatcagtt
     8401 gttctacaac accgggattt ccacctacgt atggatcctc tcgaaaaaca aggccaagaa
     8461 acggcaccgg aaagtgcagt tgattgatgc caccgggtgc tggacagagc agaaaccgaa
     8521 atcactgggc aacaaacgca aagaaatcac ccccgaacac cgggccgaga ttgtaaaact
     8581 ctaccgcggg ttcaaggaag gcgaccgggt gaagattctc gaaacggagg acttcggcta
     8641 ccacaaagtg accgtcgaac gccccctgcg cgacgaacag ggcaacattg tcctgaaaaa
     8701 aggaaaaaaa caggccgatt ccgcgttgcg cgacaccgaa aacgtgccgc tgaaggaaaa
     8761 aatcgccgac tacatcgccc gcgaagtgct gccctacgct ccggatgcgt gggtggacga
     8821 ttccaagacc aaaatcggct acgaaatccc cttcacccgc tatttttacc ggtacgcccc
     8881 ccccagaccg tcccgcgaca tccttcaaga agtcgtcgcc atcgaagatg acatccgtga
     8941 caccctcgcc agcctgctcg gaaggaacgg caacccatga aaccccgcaa aatgaaggac
     9001 agcggcatcc cctggattgg cctgatcccg gaggggtggg aagcgcgcaa gattaagacc
     9061 atttttcaag tgcttggagg tgcaactccg gcaagcggga atgttgacta ttgggacggc
     9121 gatattccgt gggtaacgcc ggccgatatg agtgatgata aaatttattt gaaagactcc
     9181 aaacgaaaga taacacgaga aggtcttgag tcatgcgcgg cagaactcgt ccctgtcgaa
     9241 tcaataattg tttcaaatcg tgctccgatt ggaaaagttg cattggcggg cgtaccacta
     9301 tgcaccaacc aaggctgcaa gagtcttcgg gcatctgaat ccgtatgcag taaatatttc
     9361 tattattatt tgcggactca aacagaaacc ctgaacatgt ttggccaggg gacgacattc
     9421 ttagagcttt ctagtcgatc gttgaacgat tttcttgtcc cgtatcctgc agaaaatgaa
     9481 caatcccgaa tcgcggccta tctggacgaa aagtgtggtg aaattgatcg ggtcattgca
     9541 gcgaaggagc aacagaacaa actgttgcgc gaccaacgca ccgccatcat cagtgaggcg
     9601 gtcacaaagg ggctcaaccc caatgcaaaa ttcaaagata gcggatttga atggcttggg
     9661 gagatacccg ttgattggtg ccatttaccg ttgcgatatg cggttgtttc cattaccgga
     9721 ggtgcgacgc ctcaaaaaga gaatccacta ttctgggacg gcgatattcc ctgggcatct
     9781 tcaaaagatt taaaaaccaa caagatttac aaaaccgagg attcaattac aaaggaagga
     9841 ctcgaacgct gctcggtttc actcattgac gcagggcggc ttatcctgtg tgcccgttct
     9901 ggcattcttc gccataccct gcctgcatgc gtcaatatgg tgccgctaac aataaaccag
     9961 gacctgaagg cactcgaatt cagaaaagaa atcaacatct actatgttca gtattacttt
    10021 ctgggtttga accgggagat tctgaccttc agtcaaaagg aaggcgccac tgttgaaagt
    10081 ctcaatatgg agttgctcat gaaattgccc atcttttttc cgcgaatgcc tgaacaagag
    10141 aagattgtgt catatctgga ttcccgttgc gcggagatcg accaggtaat cgcggcaaac
    10201 gagaaaatgg tggccaagtt gaaggaatac cggagcagcc tgatttggga agccgtgacg
    10261 ggaaaaacca gtttgtagtc ccgcctttag gcggaaggat cggaagatgc cgcctgaagg
    10321 cggtactacg agccgggagc tccgcctgaa ggcgggacta caaacccaaa gctccgccta
    10381 aaggcggtac tacaaacgaa gtaaaaaaag gaatacacga tgtaccagtg gcgaaaaatg
    10441 acggtggagg aacgagaaga ggcgttgcgc gagcggcgcc aacgcaggtt gccgcatcac
    10501 agtccgccgc attttgcggg cgatgcgcca cggctttatc acctgacggc ggcctgctac
    10561 gagcatcgcc ccattgtggg gctttcgccg aatcgcatgg cggaattcga ggccgagctg
    10621 gtcaaaaccc tgcgcgccga gggccgcgaa ctggcggtct ggtgcgtgct gccgaaccat
    10681 tggcatgcgc tggtgcgcac aggggatctg cgtgggacga ccaaggctgt gggccgattg
    10741 catgggagaa tgtcgcacgc gtggaacggg gaggcggatt gccgcgggcg gacatgctgg
    10801 catcgctgcg cggaccgggc catgcggagc gatgcgcatg tttttgcaac gatcaactac
    10861 atccatcaca atcccgttca tcatggatat gtggagcaat ggacgcagtg gcccttttca
    10921 agcgcggcag actatctgga cgccataggc caggaggaag cgaaacggcg ctggaaacaa
    10981 tatccacttt tggattacgg aaagggctgg gacgagccgg agatgtgatc aatgttcgta
    11041 gtcccgcctt taggcggaag gatctaaaac cgccgcctaa aggcggaact accaacccga
    11101 aatccccgcc taaaggcgga actacaaact cgatccaaga ccgcctgaag gcggaactac
    11161 gagccagaaa acccccgctt gcgaaagcgg agggaaggct gataggatgc ggactgaacc
    11221 agggcgtgga caactggatg ggatgaggta aggcgatggc ggcagaagat ttaagagaaa
    11281 atccgttccg ggacgagatt tgcgccgcgc tggttgccaa gggctatgtc gagggacaaa
    11341 agggggatta cgtaatggat cgcgcgctgg acagggcgcg attgctggaa tttctggagc
    11401 ggacgcagga gccggagctg gccaagttcc gggcggcgca cggggccgat tggcaaaacc
    11461 gcctgttgcg gctgtgggac gagaccatcg aacataatgg cctgatcaaa acgctcaagg
    11521 ggttggtgga ggattatccg agcggcgcca aattccatgt tgtggatttt ccccggaatc
    11581 tggcggggat gggagcgggt tcgctcgaaa agaacgtgtt ttcggtgcgc cgggagttta
    11641 tctacgagaa caagccggaa ccgcaccggg tggatttggc gatcttcctg aacggcattc
    11701 ccgtggcgat gatcgaactg aaaaaacgca cggcggggca aagcgcgggc gttgagggca
    11761 tgcggcaata ccgcaacacc cgcgatcccc gggaaaaggt gttcggtttc aaccgacgca
    11821 ctttgttcta cctggtgatg gatgaattcg aggcctttgt gtctacgcgg ctggccgggc
    11881 cggaaacgaa attcttgccg ttcaaccggg ggacggcgac gggcggtgca ggcaatccca
    11941 ttgaggaggg caaacacccg acgcaccatg tgtgggatga actgctcgag cgcgacatgt
    12001 tgctgcggat cattcgcgac tacctgttta ttgacgaaga agggaagatg atttttcccc
    12061 ggtatcacca actggacgcg gtactgaaac tcgaacggaa cgtccgggaa catggaatgg
    12121 gcggccgcta tctggtgtgg catagcgccg gtagtggcaa aaccaagacc atctcctggc
    12181 tggcattccg cctgctcaac cttcccggaa tcaaaaccgt gatcgtgatc agcgaccgca
    12241 aggtgattga ctcgcaactg gccggccaag cactgacgat ggatggcaag acgggccggg
    12301 tgcaatgggc ggaggatagc gccgagctgg cggcattgct gaacgagggc ggaatgattg
    12361 tcgtgacgac cctgcagaaa ttccccctgg ttctggaaaa actccaggag aagactggcg
    12421 agcagtacgg gatcatcatt gatgaagcac attcctcaac cgcgggcaag acgatgtcga
    12481 agctgtccga aaccttggcc ggaaagagct tggcggaagc ggcggaactg gaacaggcct
    12541 acgaggaaag agaggacggg caacggcagt tacaggaact ggaaccccgc atcacagcaa
    12601 cgaaaaacgt gagctatttt gcgttcaccg caacaccgca aacaaaaacc atggagttgt
    12661 tcggaacccg gaattcaagg ggtaaagact gtttccacaa gtattcgatg cggcaggcga
    12721 tcgaagaggg attcattcta aatcccatcg cttgctacac ggtgtattcc gacaagctca
    12781 aagtccggaa gaaggacgag gcggaaggag aattcgagag tgcgcggacg gcggccgcga
    12841 ttctgcacta tctgaccagt tcacccgagg tggtcgaacg caaaacagag atcatgctga
    12901 ccgattttct ggcgcggcgc cagcattggc tgggcggaac ggcaaaagcc atgatcttga
    12961 cggcgtcgcg caagcatgcg gtgtgctaca aactggccgc ggatcgcttc ctagaagaac
    13021 ggggctgcga tgtcaaggcg gtggtgggct tcactgggag catcgtgctg gatgataaaa
    13081 cctataccga ggagaacttg aacgccgggc tggagggccg ggatctgcgg cagatcattc
    13141 agaaagaccc ccgcgcgcgc atcatcgtgg tggccgacaa gctccaaacc ggcttcgacg
    13201 aagaccggct gtgcgtcatg tacattgaca aacacctgaa cgggccggtg aagaccgtgc
    13261 agacgatctc gcgcctgaac cgcgcgcgcg cgggcaagcg caccttcgtc atggactttg
    13321 tgaacgaggc ccgggacatc aaagccgcct tcgaattcta ctacggcggc gagctgtggc
    13381 ttccggagga aaacgaaatt gatcccaatg tgcttttcca gaagcggaat cgtttgctgg
    13441 aatatggcgt gttcacgatg gaagaagccc ggcgggccta tggattcatc gtcgccgaag
    13501 agaaacattc gccggaattg agcgcgttgt tcgccgagct gatcggacgc cacgcccgct
    13561 tgccggttac tcccaaggaa aataagaaaa agttgttttt ggcggaggcg agccaattcg
    13621 tttcactctt ttactatgtc tccacggttt atgcccgctg ggacttggaa atgaagcgcc
    13681 tggcggcctt tctggacacc ctgttgaatc tgctgaaggc gggcgaggat caagagcaaa
    13741 tccgccccga aaagctggcc gagctggtga agttcaccac ccggaaagtg ttgcatgagc
    13801 aaaacctgct ccctgaatcc gttgcgcaag aattggattc cgtatccacc gaagtccggg
    13861 tggccgaacc taaagtatcc gcgctggatg aaatcattca gcggatcaac gccaaatatg
    13921 gctatgagca ggccaaggat gcaattgagt ccattgtgac cacactggcc gcagatgctg
    13981 gactcgccat gaatgtgcgc aatagtgcca agagtgccta cgaggccgag acgatggatc
    14041 gcctgaacga aatctgcgtc aacgggatgc tgtcttcaga gggtgaacag gaacgcttct
    14101 atgccgagtt aagcaaagat aaggaatttc tccagaatct ggcttttgaa gtgatccgcc
    14161 gaattcagca aatccctcag gctgtttagc gatatagaaa ttttggggtc agcccttaac
    14221 aattaacaat ctccgtcctg ttcccctctc gaggcctgca tgggcggagt tggcgaaggg
    14281 atcttcgcgc gcggtgggcg tggcgtgggg gaaaaagtgg tcggggcgac gagatttgaa
    14341 ctcgtgacct tttgcacccc aagcaaacgc gctaccagtc tgcgctacgc cccgaccaaa
    14401 ggcg
//
LOCUS       DAOPNP010000058        14370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4936_length_14370_cov_7.972057, whole genome shotgun sequence.
ACCESSION   DAOPNP010000058 DAOPNP010000000
VERSION     DAOPNP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4936_length_14370_cov_7.972057"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..154)
                     /locus_tag="PKI64_06625"
     tRNA            complement(79..154)
                     /locus_tag="PKI64_06625"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Glu,seq:ctc)
     gene            270..346
                     /locus_tag="PKI64_06630"
     tRNA            270..346
                     /locus_tag="PKI64_06630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Arg,seq:cct)
     gene            404..1012
                     /locus_tag="PKI64_06635"
     CDS             404..1012
                     /locus_tag="PKI64_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOE00721.1"
                     /translation="MVWREVIVEPLLAGLATGIFCCGTCFPFLLPIFAAEQRTARQTI
                     KVWLQLMGGRLTGYLLMGALVGLLGEQLRAAWLERVMTAGMMLMALVLIFYALGFRRP
                     RWSFCAAGTGRAASAPILLGLLLGLQACPPFLLSVAYVFMLHSVAKGLVYFAVFFGAT
                     SLYFLPLIGVGWLGRLAEFRWAARLSALGVGILFFVYGMLKL"
     gene            1009..2142
                     /gene="amrS"
                     /locus_tag="PKI64_06640"
     CDS             1009..2142
                     /gene="amrS"
                     /locus_tag="PKI64_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOE00722.1"
                     /translation="MKKALAAFFLVGVLGLAAALIWVREKPAALTKVAAPAPADTNRL
                     HEASWYDKLPDGMVHCRLCPNSCRLPEGALGLCRARQNIGGTLYSLVYGQMAAVHVDP
                     IEKKPFFHVLPGGRAFSLATSGCNMRCLFCQNWEISQALPGELQTTAATPEEVVAAAV
                     RSGAQAIAFTYNEPTIFYEYMLDIARLAKARGLKTLVISNGYIQPEPLRALLPHIDAY
                     KVDFKAFSPQFYTEVTGGSREPILETMKIIHEAGVWLEIVNLLITGLNDSEDEVRELA
                     RWVRDNLGPDVPVHFTRFHPLYKLANLPPTPIERIVRARDIAMAEGLHYVYTGNIPSS
                     PGDSTYSPQTGEVLIRRQGFFVLENRLTNGVAPSGEVIPGVWQ"
     gene            complement(2090..4372)
                     /locus_tag="PKI64_06645"
     CDS             complement(2090..4372)
                     /locus_tag="PKI64_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00723.1"
                     /translation="MRKALAVSFIGLGITSVMAQVLLVRELLIVFYGNEFFIGWTLFA
                     WLVWTAAGVGVGGRVGQAPSGALRWLVVSHAATAVLLPGLLALIRAARTLLGVLPGSL
                     PDLLPAMAFSLLVFAPLGLVLSAHFVVATRLWPAGAANGRPDAGGLGWGYVCETIGFV
                     LGGVLFSFVFATASELRTLVLLGCLNALTGFGLCRGFHSRAGSLRVVLLMALAALLVL
                     STHARRWEWRTAAWRYPGQLLVAARTSIYGHLAVTAIGRQINFHENGALLGAEDEQQA
                     AENLVHIPMLWHEQPRRVLLIGGGFNGALGEILKHQPAHVDYVELDAALIALARQYAA
                     PSRQQALIDPRVHPIFADGRAFLNALAADPAAEPYDVVILNLPGPSTVLLNRYYSREF
                     FRDVRRLLASDGVLAVRLPFAPDYLCRELENLGVSIDRALRAEFPAVSLLPDYDLLYV
                     ATPSAAPAPTAATLQARYQVRGFRTEFVIPPAIAYRLTTDRVTQTAALFDRNTDARLN
                     RDSRPVACVYSFAYWLRLFHPRVADAVLRASARSWGWGAGGLLLLLLLMGSAVGARPA
                     HLGPWAMGVGGFTMMGAELVLLLGFQIVHGHLYYRLALLLAVCMAGMALGGWWGLRRT
                     ATATPRTLAKLHAVLAGYLLIMAGVLRLMAVGTWQHGTGGELAFLLLAVGVGIVVGWE
                     FPLATRLHWAANPAAAARGAVYGADILGSCLGALLVSLWALPLYGIPATLLGLAALNL
                     GMAAIARLPGSLRRMAPRHS"
     gene            4538..5320
                     /gene="udp"
                     /locus_tag="PKI64_06650"
     CDS             4538..5320
                     /gene="udp"
                     /locus_tag="PKI64_06650"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOE00724.1"
                     /translation="MAKKTKRPAKPAAKKKEELQYHVHLKRGDVARYVLLPGDPGRVP
                     LIASFFDNAKEMAFNREYRTWTGTVDGIKISCCSTGIGCPSTAIAVEELIKCGADTFI
                     RIGTCGALQREVKLGDLCITTGAVREEGTSRQYVPLSYPAVADLDVTLALRQAARNLK
                     ISAHTGIGHCKDAFFIEDKDIPIREDIDAKWNAWYRSNVISTSMESAALFVVSSIRRV
                     RAGEVLATIGLTYDDAPIIAKVGIEEAIRTAIEAVKILERQR"
     gene            5333..6394
                     /locus_tag="PKI64_06655"
     CDS             5333..6394
                     /locus_tag="PKI64_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00725.1"
                     /translation="MSTPKKSAHPLHRIPLEKWQALYDLAFRLWDMAPWEQMEETEIF
                     GIQPAASPEPWFVSVMGAIGEFRAITFYDGMEGLRLFWQIQTTPGNSLRDSGQVLNTP
                     QLMAAFGEAGDIFPEEKKLLKQIGYAPRGKRGWPYFRSYRAGYAPWILEPAEADCLLT
                     CLTKALEFLPQYLADPGLLPPTAPGADVLVLVEKADGWARQLVKPTIPLVRYDFKLPM
                     AVIHQVRDIPRTTHTFELGLWPLPMYTGEKNERPQIPYMLLLTDRESSFILGAQLLSV
                     NDDGGWPGMMAKIPDHLMETLLKHKVRPTRIHVSTPWLEKLLDPPGRATDVYVEYRED
                     MSAQMAVLGELLNSPPFQQ"
     gene            complement(6427..7122)
                     /locus_tag="PKI64_06660"
     CDS             complement(6427..7122)
                     /locus_tag="PKI64_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE00726.1"
                     /translation="MKRLGLILAPHPDDECITGLLPLRLQEEAGFTICAVPVTLGSNR
                     ARQAARRKEFIAACAVLGFKPLAMPSGAPEEAIAKLLCRWQPGILLMPHSRDGHPTHR
                     QVHRWGVAALDAVGGSWRVVETEYWHPLTRPNLLVAARGGQLARLRRALACHVGEIAR
                     NDYAARLPAWMSDNVRRGAELVGAPGAAAPRVAQATLYRARIRAGGRWQPAPRGRFIV
                     SRQNLCDWLAAWD"
     gene            7227..7988
                     /locus_tag="PKI64_06665"
     CDS             7227..7988
                     /locus_tag="PKI64_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE00727.1"
                     /translation="MRLMLYNMRYATGTGFKFHLPVPFGGFFRRTNKNLDALIQFFRE
                     QKADVLGLVEVDDGSYRTGHRSQAEAIARQLGKRTFCRTKYSYQSWWSRLPIMRKQAN
                     ACLSGPAVLRERTHFLRKGMKRLVMELELEDVIIYIVHLAVGRRCREAQLADLARLVK
                     RSRKPYLIAGDFNAFAGRIELQSFMQELRLASANYANLPTFPSQSPRWQLDFILHSPE
                     IRVTRFEVARHVQLSDHLPLICDFEVRAQAKAASA"
     gene            8419..9003
                     /locus_tag="PKI64_06670"
     CDS             8419..9003
                     /locus_tag="PKI64_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00728.1"
                     /translation="MKKFAIFLVLVTLALPAVRADIQTDWAYHSSGHSGRYTDLVPGE
                     DFCFLPNGAEAIPNDDTITLYVLTAHDFAGPADEQIFVRWWDGRMSHWIMGVWVKNIT
                     LHAEPEGPTLRDLPAEGSVLLDLWKVDIPAWVTQPGDNFYAIQLKGYSPDGMDERYLL
                     CKPGGDFSQTNNVGQIWSASEEFDGQDWRVEVLP"
     gene            9139..9417
                     /locus_tag="PKI64_06675"
     CDS             9139..9417
                     /locus_tag="PKI64_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00729.1"
                     /translation="MSFSKRSLGQSLKYRWGVVGVIFAVAGLIWFANSTGIMRNTKDV
                     QENLNVKIVCPRCEGEPGRKENCALCRGTGYLWVDKTKYLPGEIESVE"
     gene            9457..10656
                     /locus_tag="PKI64_06680"
     CDS             9457..10656
                     /locus_tag="PKI64_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOE00730.1"
                     /translation="MKLVSRYLLSNMFRPWCYIMAGFGIVAILVDLFGNFVDFMEAGT
                     PLRQVLLYYAVLLPTFLPYLLPVSLLLALLYALWQLGKNSEIIAMRASGLSLTQLLMP
                     YLMTGLLASFFLLAVNELFNPWATHWTRQFLRLQGAKHTNSTFAALDLMYKNVAGRRT
                     WRIRSFDPRASASFEMRDINLVQQRPDGSDEYQMDVDRARWLGSHWWFETVQTRYYST
                     SNQPLGRKESAASMEMTMLSETPQDFVNEIKDTTERSASEIRQFIDSHQGISHKTRNR
                     LMVDFHYRLAAPWLCLLVILVGVPFGMFTGRRGMGHGILYALLAFFGYYILMGLGLAY
                     GKERILSPVLAGWLPDIVSPVLAGWLPDIVFHIRQLHAAARPVLAGWLPDIVFLALGL
                     YLLRRVR"
     gene            10744..11964
                     /locus_tag="PKI64_06685"
     CDS             10744..11964
                     /locus_tag="PKI64_06685"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOE00731.1"
                     /translation="MKTKITREFLEKIPKTDLHLHLDGSLRLNTLIELAKAAKVPLPS
                     ETESGLQELVFKEKYQDLPDYLRGFGLTCAVLQTPENLERVAYELAVDSMAENVRYIE
                     VRYAPQLHIRPGFTVEEVIECVNRGLARAQQEHNASAAVKDGRDIPFHYGIITCAMRF
                     FNRHMSAYYAQLFDVMPHTPAKEVMAIASLEMARAAANLAHTKGIPVVGFDLAGAEAG
                     FPAIDHKEAYQYAHVHFLKKTVHAGEAYGPESIFQAITECHADRIGHGTFLFAADKIK
                     DPAIKDPEYYVRCLVEHVASRRITLEVCPTSNLQTIPAITSVAEHPLRKMLEASLSVS
                     ICTDNRLVSRTSVTRELELVCSQLPVTPAQLRNIILAGFKGSFFPGPYSEKRALVRQV
                     INRYEALEKQYLKA"
     gene            11973..12740
                     /locus_tag="PKI64_06690"
     CDS             11973..12740
                     /locus_tag="PKI64_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE00732.1"
                     /translation="MRRWRLPILLLLGTALAGALGCRSIRAPTVGTLLIGVRADAPPL
                     CFRAKGQWQGVEVELGRALAARLNLQPVFKAYPAEDLTDALLNGKVDVIMAGLPITPE
                     KRLLMDFASPYLVSGQAAIVRTPDLPHANTLIKIRSARIRIGVLADSPGAEYAARYFT
                     RATRQPFPDAAAAVDALRAGQVDLVVHDAPAAWWLTLRHAPHLALAPVLFDRVELAWA
                     FRRSSVGLRESANQALAAWQQDGTLAAILRRWVPFSS"
     gene            12759..13970
                     /locus_tag="PKI64_06695"
     CDS             12759..13970
                     /locus_tag="PKI64_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOE00733.1"
                     /translation="MDASLLIIGAGPAGLLAALAAARRNLRHGVLVLERLLTPGVKLA
                     ATGGTRGNLSHHADEEEFAAAFGRAGRFTLPAFRALPPAALQELLAAHGCPTVVDNTG
                     RIYPRSQSAAQCRDALYAAAQRVGVRFVLGHPVRTIRPPDSPTAPWRVDGWAAHSVLL
                     AAGGQAAPQLGSDGSGFALARDLGYTITPPAPALTALYTTETWPATHSGLSLPQVTLT
                     IDAPRTTPSHGEILFTHRGISGPAALNLAGQVARLLLRQQTVTLRLNLLTSPVDVARL
                     RQTAGASLVLTWLAQQVPRSLAHTLLQLAGIPETTPFARLTAQQQERLRTTLHALPLT
                     VRATGGFRESMVTAGGVSLKQVIPDTLAGRLTPRLHFAGEILDLAGPTGGWNLQWAFC
                     SGHLVGSLAAE"
     gene            14099..14183
                     /locus_tag="PKI64_06700"
     tRNA            14099..14183
                     /locus_tag="PKI64_06700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14133..14135,aa:Leu,seq:tag)
BASE COUNT         2794 a         4611 c         4298 g         2667 t
ORIGIN      
        1 tcgcagacct tccagccgct cccggcggga aggtttcatt cctcaaccat tcccgtccga
       61 ccaaagtatt ctgcgagttg gcacccccaa ccggattcga accggtgtta ccaggatgag
      121 aacctggcgt cctaggcctc tagacgatgg gggcgcagag gaattggacg gggactttgc
      181 cccagcaggc ggggtttgac aatcaaaact ttcgcggcca gcgcaagctt gaacgagccg
      241 gcgaatggtg gttgaatcct gcgcaacagg ccccggtagc tcagttggac agagcggcgg
      301 tttcctaaac cgcaggtcgg aggttcgatc cctctccggg gtaccatcga ggccggcgat
      361 gaaagtttcg tttccatttg ggtgcggggt ggcgggggcg tagatggtgt ggcgcgaggt
      421 catcgtggag ccgttgctgg cggggctggc gacggggatt ttttgctgtg ggacgtgttt
      481 cccttttttg ttgccgattt ttgcggcgga gcagcgcacc gcgcggcaga ccatcaaggt
      541 ttggttgcaa ctgatgggcg ggcggttgac gggctatttg ctgatggggg cgctggtggg
      601 gttgctgggc gagcaacttc gcgcggcgtg gctggagcga gtaatgacgg cgggcatgat
      661 gctgatggcg ctggtgctga ttttttatgc gctgggattc cggcgtccgc gctggtcgtt
      721 ttgcgcagcg ggcacgggcc gcgcggcaag cgcgccgatt ttgctggggt tgttgctggg
      781 gttgcaggcg tgtccgccat ttttgctgtc ggtggcgtat gtgttcatgc tgcatagcgt
      841 ggccaaaggc ttggtatatt ttgcggtctt ttttggcgcg acgtcgctgt actttctgcc
      901 gctgatcggc gtgggctggc tggggcggct ggcggagttc cgctgggcgg cgcggttgag
      961 cgcgctgggg gttggcatcc tgtttttcgt ttatggtatg ctgaagctat gaagaaggcg
     1021 ttggcagctt tttttctggt gggtgtgctg gggttggccg cggcgctgat ttgggtgcgc
     1081 gagaaaccgg cggcgctgac caaggtggcg gcgcccgcgc cggcggatac caaccggtta
     1141 cacgaagcaa gctggtacga caagttgccg gacggtatgg tgcattgtcg gctttgcccg
     1201 aattcatgcc gtttgccgga gggtgcgctg gggctgtgcc gcgcgcggca gaacatcggc
     1261 ggcacgctgt attcgctggt ctatggccag atggcggcgg tgcatgtgga cccgatcgaa
     1321 aagaagccgt ttttccatgt gctgccgggg gggcgggcgt tttcactggc gacatcgggt
     1381 tgcaacatgc ggtgcctgtt ctgtcagaat tgggaaatct cccaggcgct gccgggcgag
     1441 ttgcaaacca ccgccgccac gccggaggaa gtggttgcgg ccgcggtgcg cagcggggcg
     1501 caagcaatcg ccttcaccta taacgagccc acgatttttt atgaatacat gttggacatc
     1561 gcgcggctgg ccaaagcgcg cggcctcaag acgctggtga tcagcaacgg ctacatccag
     1621 cccgaaccac tgcgagcgct gctgccgcac attgatgcct acaaggtgga tttcaaggcg
     1681 ttcagtcccc aattctacac cgaagtgacc ggcggcagcc gcgaaccgat tttggaaacc
     1741 atgaagatta ttcacgaggc cggcgtgtgg ttggagatcg tgaatttgct gattaccggg
     1801 ctcaacgaca gcgaggatga agtgcgtgaa ttggcgcgct gggtgcggga caacctgggg
     1861 ccggatgtgc cggtgcattt cacgcgcttt cacccactct acaagttggc caatctgcca
     1921 cccacgccga tcgagcgcat tgtccgcgcg cgagacattg cgatggcgga gggactgcac
     1981 tatgtctata cgggcaacat ccccagctcg cccggtgact cgacctactc cccgcaaacc
     2041 ggcgaagtac tgatccggcg gcagggattt ttcgtgctgg aaaaccggct cacgaatggc
     2101 gtggcgccat ccggcgaagt gatcccggga gtctggcaat agcggccatg ccgaggttga
     2161 gcgcggccag gcccagcagc gtggccggga tgccgtagag cggcagcgcc cacaagctca
     2221 ccagcaacgc cccgaggcag gagcccagaa tatctgcgcc atagaccgcg ccgcgggcag
     2281 cagccgccgg gttggcggcc cagtggaggc gcgtggccag cgggaactcc caccccacca
     2341 cgatgcccac gcccacggcg agcaacaaaa aggccaactc gccgcccgtg ccgtgttgcc
     2401 aagtgccaac ggccatgagc cgcagcaccc cggccatgat gagtaaatag ccggccaaga
     2461 ccgcgtgcag tttggccagt gtgcgagggg tagccgtcgc cgtgcggcgc aatccccacc
     2521 acccgcccaa ggccattccg gccatgcaga cggctagcag cagcgccagc ctgtaataca
     2581 agtggccgtg cacaatctga aagccgagca acagcaccag ctccgcgccc atcatggtaa
     2641 agccgcccac gcccatggcc cacggtccca agtgcgcggg acgcgcgcca actgcgctgc
     2701 ccatcagcaa caacagcagc agcaacccgc ccgcgcccca cccccacgac cgggcactgg
     2761 cacgcaatac ggcatcggcc acccgcggat gaaacaggcg cagccaatac gcaaaactat
     2821 acacacaggc cacggggcgt gaatcgcggt tgaggcgggc atccgtgttg cgatcaaaaa
     2881 gcgcggccgt ctgggtcacg cggtcggtgg tcaggcggta cgcgatggcc ggggggatca
     2941 caaactccgt gcggaagcca cgcacttggt aacgagcttg caaggttgcc gcggtgggtg
     3001 cgggtgcggc ggacggcgtg gccacataga gcaaatcgta atccggcagc aacgacaccg
     3061 ccggaaattc cgcccgcaag gcgcggtcaa tggagacgcc cagattttcc agctcgcggc
     3121 ataaatagtc cggcgcaaac ggcaggcgca cggccagcac accgtcggac gccagcaacc
     3181 ggcgcacatc gcggaaaaac tcgcggctat aatagcgatt aagcagcact gtgctggggc
     3241 cgggcagatt gagaatcacc acgtcgtagg gttccgcagc ggggtcggcg gcgagggcat
     3301 tcaggaacgc gcggccatcg gcaaaaatgg ggtgtacacg cgggtcaatc aaggcctgct
     3361 gccgggaagg cgcggcgtac tgccgggcca gcgcgatcag cgcggcatcc agctccacat
     3421 aatccacgtg cgcgggctgg tgcttcagga tttcccccag cgcaccattg aagccgccgc
     3481 caatcagcag cacgcggcgg ggttgctcgt gccagagcat ggggatatgg accagatttt
     3541 cggcggcttg ttgctcatct tcggcgccga gcaaagcgcc attttcgtgg aagttgatct
     3601 gccggccaat ggcggtcacg gccaagtggc catagatgga ggttcgcgca gccacgagaa
     3661 gttggccggg ataacgccaa gcggcggtgc gccactccca acgacgggcg tgggtggaga
     3721 gtaccaacag cgcggctagc gccatcagca acacgacgcg caggctcccc gcacggctgt
     3781 ggaagccccg gcagagccca aacccggtga gggcattcag acagcccagc agcaccaacg
     3841 tgcgcaattc gctggcggtg gcgaacacaa aactgaacag cacaccgccc aaaacaaagc
     3901 cgatggtctc gcatacatag ccccagccca agccaccggc gtcaggccgt ccgttagcgg
     3961 cacccgccgg ccacaggcgg gtggccacca caaaatgcgc gctcaacacc aatcccagcg
     4021 gcgcgaatac cagcagggaa aaggccatgg ctggcagcag atcgggcaaa ctgccgggca
     4081 gtacgcccag caacgtccgc gccgcgcgga ttaacgccag caaccccggc aataaaactg
     4141 cggtcgccgc atggctaacc accagccacc gcaaagcgcc ggatggcgct tgccccacgc
     4201 gcccccccac ccccacgccg gcggctgtcc acacgagcca cgcaaacaac gtccagccga
     4261 tgaaaaactc gttgccgtaa aacacgatca acagctcgcg caccagcagc acttgggcca
     4321 tcaccgacgt gatgcccagc ccgatgaacg aaacggccag cgccttcctc atacccgcag
     4381 tatggcccag ccgtcgcgca aaaggaacct tttccggcgc gcgcggcggc gcgcgggcaa
     4441 aaagccaata ggctccgctc aaaaatactt ggcggtagca gggctttgcg ccaaactgct
     4501 tttttgtagt gcaacccatt ccagcaaagg aaataccatg gccaagaaaa cgaaacgtcc
     4561 tgccaaaccc gccgccaaga aaaaagaaga gctgcaatat cacgtgcatt tgaaacgggg
     4621 cgatgtggcc cgctatgttt tgctgccggg cgatccgggg cgcgtgccat tgattgcgtc
     4681 tttcttcgac aacgccaagg aaatggcgtt caaccgggaa taccgcacct ggaccggcac
     4741 ggtggatggc attaagattt cgtgctgctc gacgggcatc ggctgtccct ccaccgcgat
     4801 tgccgtggaa gagttgatca agtgcggggc ggacacgttt atccgcatcg gtacctgcgg
     4861 cgcgctgcaa cgcgaggtca aactgggcga cctgtgcatc actaccggcg ccgtgcgcga
     4921 ggaaggcact tcgcggcaat atgtgccgct gtcctacccg gccgtggcgg atttggatgt
     4981 gacgctggcg ctgcgccaag ccgcgcgcaa cctcaagatt tcggcgcata ccggcatcgg
     5041 gcactgcaag gatgcgttct tcatcgagga caaggacatt cccatccgcg aggacattga
     5101 tgccaagtgg aacgcgtggt atcgctcgaa tgtgatttcc acctccatgg aatcggcggc
     5161 gctgttcgtg gtgtccagca tccgccgggt gcgcgcgggc gaagtgctgg cgacgatcgg
     5221 cctgacctat gacgacgcgc ccatcatcgc caaggttggc attgaggaag ccatccgcac
     5281 cgccattgaa gccgtcaaga ttctcgaacg ccaacgctaa ccggccacga ccatgagtac
     5341 cccaaagaaa tccgctcacc cgttgcaccg cattccgctg gaaaagtggc aggcgctgta
     5401 tgacctggcc ttccgcttgt gggacatggc gccgtgggag caaatggaag aaacggagat
     5461 tttcggcatt cagccggccg ccagcccgga gccgtggttc gtcagcgtca tgggcgccat
     5521 cggcgaattc cgggccatca ccttctatga cgggatggag ggtcttcggc tgttttggca
     5581 gatacaaacc acgccgggca attccctcag ggactcgggc caagtcctca acacgccgca
     5641 gctcatggcg gcttttggcg aagccgggga catcttcccg gaagagaaga agttgctgaa
     5701 acaaatcggc tatgcaccac gcggcaagcg gggctggccc tacttccgga gctatcgcgc
     5761 gggctatgcg ccgtggattc tggaaccggc ggaggccgac tgcctgctta cgtgcctgac
     5821 gaaggcgctg gagtttctcc cgcagtactt ggccgatccc ggcttgctgc caccgaccgc
     5881 gcccggtgcc gacgtgctgg tgctggtgga gaaagccgat gggtgggcca ggcaattggt
     5941 caaacccacg atccccttgg tgcgttacga ctttaaactg ccgatggcgg tcatccatca
     6001 ggtacgagat attcctcgca ccacgcacac gtttgagctg gggttatggc ccttgcccat
     6061 gtacacgggc gaaaagaatg aacggccgca aatcccctac atgttgttgc tcacggatcg
     6121 cgagtcgtcg ttcatcctcg gcgcccaact cctctcggtg aatgacgacg gcgggtggcc
     6181 gggcatgatg gcgaaaatcc cggaccacct gatggagacg ttgctcaagc ataaggtgcg
     6241 ccccacccga atacacgtca gcacaccatg gctcgaaaag ttgctcgacc cacccggccg
     6301 ggcgaccgac gtctatgtgg aatatcggga agacatgtcg gcacaaatgg cggtcttggg
     6361 agagttgctg aattccccgc catttcagca gtgagttcta cgcggcgcca gcctgaaaaa
     6421 atcaggctaa tcccatgcgg cgagccagtc gcacaagttt tggcgtgaca cgatgaagcg
     6481 cccgcgcggg gcaggttgcc aacgcccgcc agcgcggata cgcgcgcggt acagcgtggc
     6541 ttgggccacg cgcggcgcag cggcaccggg tgcacccacc aactccgcac cgcgccgcac
     6601 gttgtcgctc atccaagccg gcaaacgggc ggcataatcg ttgcgcgcga tttcgcccac
     6661 gtggcaagcc agcgcgcggc gcagccgggc cagttgcccc ccgcgcgcgg caaccaacag
     6721 attgggccga gtcagcgggt gccaatattc cgtttccacc acccgccatg atccacccac
     6781 ggcatccagc gccgctacgc cccagcggtg tacttgccga tgcgtgggat ggccgtcgcg
     6841 ggagtgaggc atcagcagga taccgggctg ccaacggcat agcagcttgg cgatggcttc
     6901 ttccggcgcg ccgctgggca tggccaatgg cttgaagccc aacacggcgc atgcggcgat
     6961 gaactccttg cgccgcgcgg cttggcgggc gcggttgctg ccaagagtca ccggcaccgc
     7021 gcaaatggtg aaaccggcct cctcttgcag gcgcagcggc aacaaaccgg tgatgcattc
     7081 atcatccgga tgcggggcca aaatcaagcc gagacgcttc atgccggcgt tgtatcctgt
     7141 ccgtcgttgg ggggcgagat ttttcttatc gaaacgcttg aattcccgcc ggggcggtgt
     7201 atggttcccc acatatttct tttgccatgc gtctaatgct ctacaatatg cgctatgcca
     7261 ccggtacggg attcaagttc cacctgccgg ttccctttgg cggctttttc cggcgcacca
     7321 acaagaatct tgacgccttg attcagttct tccgcgagca gaaagccgac gtgctgggct
     7381 tggtggaagt ggatgacggc tcctaccgca cggggcaccg tagtcaggcg gaagccatcg
     7441 cccggcaact gggtaaacgc acattttgcc gtaccaaata cagctaccaa tcctggtgga
     7501 gccgcctgcc gatcatgcgc aagcaggcca atgcctgcct ctccggcccg gcggtgctgc
     7561 gcgaacgcac gcactttttg cgcaagggca tgaagcgcct cgtgatggag ttggaactgg
     7621 aagacgtcat catttatatc gtgcacctag ccgtgggccg acgctgccgc gaagcacagt
     7681 tggcggacct cgcacgcctg gtcaagcgct cgcgcaagcc ctacttgatt gccggcgact
     7741 tcaacgcctt tgccggccgc attgagttgc aatcgttcat gcaggaactc cggctggcca
     7801 gcgccaatta tgccaacttg ccgaccttcc ccagccaatc accgcgctgg caactggatt
     7861 tcattctgca cagcccggaa atccgcgtga cccgcttcga ggtggcccgc catgtgcaac
     7921 tgagcgacca cctgccgctg atttgcgact ttgaagtccg cgcccaagcc aaggccgcta
     7981 gcgcctgaag cctcagcccg cgcggagcaa accaatagac tccccgccga tatttttgtg
     8041 gggacattgg ggtcggcaac tgccatgcgg gaccgtattg cacggataaa aaacgcagaa
     8101 atcattcacc ttgctcttat ccagacttta taaactctgg atggcaccct caaaacagcc
     8161 gattaaaaat tactacaaaa ttatgcctaa caatttgtat atgattgaga tatgaatata
     8221 accaaatcca tccagacttt ataaactctg gatagcatgg ttcaaatagg aaaaaaggtg
     8281 gtcagacaac cgctatttct tcagcccaag cgtgaccatc cagccactga aaaagcgagg
     8341 ggccaatcat ccgaccggtt ccgagagttg cgctggtccg ggggagtggc tacaatggcc
     8401 cgcgtttcgg aggttgccat gaaaaagttt gcgatattcc tcgttctggt cacgctggcc
     8461 ctgccggcag tgcgcgccga tatccaaacc gactgggcct atcattccag tggtcactcc
     8521 ggtcgctaca cggatttggt gcccggagag gacttttgct ttctccccaa cggcgccgag
     8581 gctattccca atgacgacac catcaccctc tacgtgctga ccgcccacga ttttgccgga
     8641 cccgcagatg aacaaatttt tgtgcgttgg tgggatggcc ggatgtcgca ctggatcatg
     8701 ggcgtctggg tgaaaaacat cactctccac gcagagccag aaggcccaac cctgcgcgac
     8761 ctgcccgcgg agggcagcgt actgttggat ttgtggaaag tggatattcc agcgtgggtc
     8821 acgcagccgg gagataattt ctacgccatc caactcaagg gttattcccc cgacggcatg
     8881 gacgagcgct atttgctgtg caagccgggc ggtgatttct cgcaaaccaa caacgtgggc
     8941 caaatctggt cggcctccga ggagttcgat ggccaggatt ggcgcgtgga ggttctcccc
     9001 tagccccccc tgccggtgcc tgtgaagcgc aaactcccct accgcccccc ccttgcgccg
     9061 gaaaagtgca cttgccgacc cgtcatgcct tgactttatt tgcgcggaac aggcacactg
     9121 cgggcattcg gaggactcat gtcatttagt aaacgcagcc tcggtcagtc actgaaatat
     9181 cgttggggcg tcgtgggagt aatctttgca gtggcgggct tgatttggtt tgccaactct
     9241 acggggatta tgcgcaacac caaggatgtg caggaaaatc taaacgtcaa aattgtctgc
     9301 ccccgctgcg aaggcgaacc cggccgcaag gaaaattgcg cgctgtgccg gggcaccggt
     9361 tatctgtggg tggataaaac aaaatatctg ccgggcgaga tcgaaagcgt cgagtaagat
     9421 gacgcggggc gcgccagccg cctcaaggat tccgggatga agttggtcag tcgctacttg
     9481 ttgagcaaca tgttccgccc gtggtgttac atcatggcgg ggttcgggat cgtggcgatt
     9541 ctggtggacc tgttcggcaa ctttgtggat ttcatggaag ccggcacgcc cctgcgccaa
     9601 gtgctgttgt attacgcggt cctactgccg actttcctcc cctatctgct gccggtctcg
     9661 ctgctgctgg cgctgctcta cgcgctgtgg cagcttggca aaaactccga gatcatcgcc
     9721 atgcgcgcca gcggcctgag cctgacgcaa ctgctgatgc cctacctgat gaccggcttg
     9781 ctggcctcat ttttcctgct ggccgtcaat gaattgttca atccgtgggc cacccattgg
     9841 acgcgccagt ttttgcggct gcaaggggcc aagcacacca actcgacgtt tgcggccttg
     9901 gacctgatgt ataaaaacgt ggccggccgc cgcacatggc gtattcgctc gttcgatccg
     9961 cgcgccagcg catccttcga aatgcgcgac attaatctgg tgcagcagcg gcctgacggc
    10021 tccgacgaat atcagatgga tgtggaccgc gcccgctggc tcggcagcca ctggtggttt
    10081 gaaaccgtgc aaacgcgcta ctacagcacc agcaaccagc cgctcggccg caaagagtcc
    10141 gctgccagca tggaaatgac catgctttcc gaaacgccgc aggatttcgt gaacgaaatc
    10201 aaggacacca ccgagcgctc cgccagcgaa atccgccagt tcatagactc gcatcagggc
    10261 atttcgcaca aaacccgcaa ccggctgatg gtggattttc attatcgcct cgccgcgcca
    10321 tggctttgcc tgctcgtcat tttggtgggc gtgccgtttg gcatgttcac cgggcggcgt
    10381 ggcatgggcc acggcattct gtatgcgctg ctggccttct tcggttatta cattctgatg
    10441 ggtctcggcc tcgcctacgg caaggagcgc atcctgtcgc cggtgctggc cggctggctg
    10501 ccggatatcg tctcgccggt gctggccggc tggctgccgg atatcgtctt ccacatccgt
    10561 caactccacg ccgccgcgcg cccggtgctg gccggctggc tgccggatat cgtcttcctg
    10621 gcgctggggc tctatctgtt gcgccgcgtt cgctaattgt gcggaaaaac cgttgcgcac
    10681 ccggcgcgag ttccgtagga tgctgtgcaa cgtgcaagca ttgagcatgg caggaggcgc
    10741 ctgatgaaaa ccaaaatcac ccgcgagttt ctcgaaaaaa tccccaaaac cgatttgcat
    10801 ttgcacttgg acggctccct gcggctgaac accttgatcg agctggccaa ggccgccaaa
    10861 gtgccgctgc cgtcggaaac tgaaagcggc ctgcaagaac tggtcttcaa ggaaaaatat
    10921 caggacctgc ccgactactt gcgcggcttc ggactgacct gcgccgtgct gcaaaccccg
    10981 gaaaatctgg agcgcgtggc ctatgagctg gccgtggaca gcatggccga aaatgtgcgc
    11041 tatatcgaag tgcgttacgc gccgcaactg cacatccgcc ccggcttcac cgtggaggaa
    11101 gtcattgagt gcgtcaaccg cggcttggcc cgcgcccagc aggaacacaa cgcctccgcc
    11161 gccgtcaagg atggccgcga catccccttc cattacggca tcatcacctg cgccatgcgc
    11221 ttctttaatc gccacatgtc tgcgtattac gcgcagttgt ttgacgtgat gccccacacg
    11281 ccggccaagg aagtcatggc catcgcctcg ctcgaaatgg cccgtgctgc cgccaacctg
    11341 gcccacacca agggcatccc cgtggtgggc ttcgatcttg cgggcgccga agccggtttc
    11401 cccgccattg accacaaaga ggcctatcaa tatgcccacg tgcacttcct taaaaaaacc
    11461 gtgcacgccg gcgaggccta cggccccgaa tccatcttcc aggccatcac ggaatgccac
    11521 gccgaccgca tcggccacgg caccttcctg tttgccgccg acaaaatcaa agaccccgcc
    11581 atcaaggatc cggagtatta cgtgcgctgc ctcgtggagc acgtggcctc gcggcgcatc
    11641 acgctcgaag tctgccccac cagcaacttg caaaccatcc ccgccatcac ctcggtggcc
    11701 gaacacccgc tgcgcaaaat gctggaagcc agcctgtccg tctccatctg cacggataac
    11761 cggctcgtct cccgcaccag cgtaacgcgc gagctggaac tggtgtgcag ccaactaccg
    11821 gtcaccccgg cccaactgcg caacatcatc ctggccggct tcaagggctc gttcttcccc
    11881 ggcccttaca gcgaaaaacg cgcactcgtg cgccaagtca tcaaccgcta cgaggcgttg
    11941 gaaaaacaat acctgaaggc ataggccacc ccatgcggcg ctggcgcctg ccaattctcc
    12001 tgctgctggg cacggccttg gccggcgcgc tgggttgccg ttccatccgt gcgcccactg
    12061 tcggtacgtt gctcatcggc gtccgggccg atgccccccc actttgcttc cgtgccaaag
    12121 gccagtggca gggcgtcgaa gttgaactgg gccgcgccct cgctgcgcgc ctcaacctgc
    12181 aaccggtgtt taaggcctac cccgccgaag acctgaccga cgcgctcctc aacggcaagg
    12241 tagatgtcat tatggccggc ctgcccatca cgcctgaaaa acggttgctc atggactttg
    12301 cttcgcccta cttggtctcc gggcaagccg ccatcgtccg cacgccggac ctgccccacg
    12361 ccaacacgct gatcaaaatc cgcagcgcgc gcatccggat cggcgtgctg gcggattccc
    12421 ccggcgccga atatgccgcg cgctacttca cccgcgccac ccgccagcca ttccccgatg
    12481 ccgccgccgc cgtggatgcc ctgcgtgccg ggcaggtgga tttggtggtg cacgacgccc
    12541 ccgccgcgtg gtggctcacg ctccgccatg ccccccacct cgccctcgcg ccggtgctgt
    12601 tcgaccgcgt cgaactggcc tgggccttcc ggcgcagcag cgtcggcttg cgcgaatcgg
    12661 ccaatcaggc gttggccgcc tggcaacagg atggcactct ggccgccatt ttgcggcgct
    12721 gggtgccgtt ttcctcctaa cccgcgcgag cccgccccgt ggatgcttcg ctcctgatta
    12781 ttggcgccgg tcccgccggt ttactcgccg cattggccgc cgcgcggcgc aacctgcgcc
    12841 acggtgtgct cgtgctggaa cggctcctca ctccaggcgt caaactggcg gccaccggcg
    12901 gaacccgcgg caacttgagc catcacgccg acgaagagga attcgccgcc gcattcggcc
    12961 gcgccggacg cttcaccctg cccgccttcc gcgccctgcc gcccgctgct ttgcaggaac
    13021 ttctcgccgc acatggctgc cccacggtgg tggacaacac cggacgcatc tatccccgct
    13081 cgcaatccgc cgcgcaatgt cgcgatgccc tctacgccgc cgctcaacgc gtcggcgtgc
    13141 gttttgtatt gggccacccc gtccgtacca tccggccccc cgactcgccc accgccccgt
    13201 ggcgcgtgga cggctgggcc gcgcacagcg tgctgcttgc cgctggcgga caagccgccc
    13261 cgcagctcgg ctccgatggc tccggatttg ccctcgcccg tgacctcggc tacaccataa
    13321 ccccgcccgc cccggccttg accgcgctct acaccacaga aacatggcct gccacgcata
    13381 gcggcctctc cctgccgcaa gtcacgctca ccattgatgc gccccgcacc acgcccagcc
    13441 atggcgaaat cctgttcacc caccgcggga tttccggccc cgccgcattg aaccttgccg
    13501 ggcaagtcgc gcggctgttg ctgcgccagc aaacggtaac gctgcgacta aacttgttga
    13561 cctcgccagt ggacgtggcg cgcttgcgcc aaacggccgg cgcctcgctc gtgctgacgt
    13621 ggctggccca acaggtgccc cgctccctcg cccacacttt gctccagttg gctggcatcc
    13681 ccgaaaccac accctttgcg cgattgaccg cccaacagca ggaacggtta cgcaccacgc
    13741 tccatgcact gccgttgacc gttcgcgcca ccggcggctt ccgcgaaagc atggtcaccg
    13801 ccggcggagt ctcgctcaaa caagtcatac ccgacaccct cgccggccgc ctgaccccgc
    13861 gcctccattt cgcgggcgag attctcgacc tcgccggccc caccggcggc tggaacctcc
    13921 aatgggcttt ttgctccggc catctcgtcg gctcgctcgc cgccgaatag taccgcgcct
    13981 tcaatcagtt attctccacc gcccccgcgc cgcacggcct gccggcacca aaatgcggtt
    14041 ggagcttgat cttcaccgga atttgacggt ctaatccccg gctgtcggca atccctccgg
    14101 acgggtggcg aaattggcag acgcgccaga cttaggatct ggtggagcaa tccttagggg
    14161 ttcgagtccc ctcccgtcca ccactttgca tattacccgc gtgtttattg gcgtttatgc
    14221 gttcttgcca tccagataaa cgccccggtg ataagggtag gtgataagat ggatatggat
    14281 aaaacgggct ctccatcaaa agacggggca aagccccccc ccgacttggg ttagaagttg
    14341 agtaacgaaa aacaaccata actcaaggag
//
LOCUS       DAOPNP010000059        14354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_4943_length_14354_cov_9.323030, whole genome shotgun sequence.
ACCESSION   DAOPNP010000059 DAOPNP010000000
VERSION     DAOPNP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4943_length_14354_cov_9.323030"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..325
                     /locus_tag="PKI64_06705"
     CDS             41..325
                     /locus_tag="PKI64_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008053560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOE00734.1"
                     /translation="MPSSNPPSGAGEKSKVAAGVLGILLGALGVHKFYLGYTQAGLIM
                     LLVSIVGGIFTCGMASSVIGIIGLVEGILYLTKSDEEFTATYVQNKKPWF"
     gene            386..655
                     /locus_tag="PKI64_06710"
     CDS             386..655
                     /locus_tag="PKI64_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HOE00735.1"
                     /translation="MGDTPNLNPQNIPNYLWQSIVVTVLCCLPLGIPAIIYASKVNNA
                     LMQGNQQAAWQASNTAKMWCIIALVAGLMGGFLYFMVFGLALIFG"
     gene            679..1083
                     /locus_tag="PKI64_06715"
     CDS             679..1083
                     /locus_tag="PKI64_06715"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOE00736.1"
                     /translation="MAGLLLAGLVLLAVLPPNVGCFPPCPVHTTTGLYCSGCGSLRAI
                     RSLLSGDVLQAWHYNPILLCCIPIILVGVLSEVVHTKRLSLTRIRPFLLWVWLSVILA
                     FGVLRNIPAFDYLQPTALSAKTIHAGALTWPR"
     gene            complement(1097..2935)
                     /gene="recD"
                     /locus_tag="PKI64_06720"
     CDS             complement(1097..2935)
                     /gene="recD"
                     /locus_tag="PKI64_06720"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOE00737.1"
                     /translation="MNQPRTCTPQQLRDANLIRDLDLFLIESLIRTAAAHPGFIEPDP
                     IVQCILTLASRVTGDGHVCLDLGQDAWWQLQGNVPAEPAEDSAGPGDDTTLANQTLNS
                     LPSPAIVLPLLENLPGLVSDGSTPTPFVLEETRLYLRRFWNYERLVAEKLRVFAQLDP
                     VAVPEEIQNAIRTYKLQPSGSPLAKGQQDAVLAALERQFTIITGGPGTGKTTIAAVLL
                     EQIAKLAPEHLRLRVRLLAPTGKAAARLDESLRSGLPEAARDRMEIPAAATIDRALGY
                     LPNSPYFRHNRDNPISADAFIVDEASMIDLPKMAKLLDAIPLSARLILLGDKNQLASV
                     DPGSVMAELCDSTLLQQKSVVELTESKRFDATSAVTPLSQAVRQQNANLAWQIASDPT
                     LGAPGKKIALHDATQIRYGDLPREFTNLVKERFADFKNAATPANAFATLAKFRVLCAL
                     RRGPAGVNNINRAIEDILFPNRRGEFYDHRVILITTNDYETNLFNGDVGIILPDPDRN
                     NQPAAFFEGRPHPVPCHLLPEHETAFAMTVHKAQGSGFNHVAVILPDRATPLLTRELI
                     YTAITRTETGVDLWTTKETFQSAVQTATTRAMGLAQKLDHPSAPVA"
     gene            complement(2932..6588)
                     /gene="recB"
                     /locus_tag="PKI64_06725"
     CDS             complement(2932..6588)
                     /gene="recB"
                     /locus_tag="PKI64_06725"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HOE00738.1"
                     /translation="MSDFSNLVFSPTHAFDTPQLVEASAGTGKTYNIQNVFLRLILEK
                     NLSVREILVVTFTNAATCELRERLRNVLLDCRYYLEAPPAGALTPEQQRIHDALQLVP
                     HKTDEEIQLLKQRVQLAIMDFDSAAIITIHGFCQRVLKHYAFECGHDPDAELMPEASH
                     IIREACQDWWRKNAYTPSPDSPNPFEKIGQLIQLVTELYKHPTATIRAAALPDTPAFQ
                     DATSACKTVFTGFKNMRGNFNWKSKAIITRVDDTVDASPVFDSIKQNPAEWDAWAAAQ
                     RELPAPRHELGESFLTIVNTASAPPTTGNAKPFVLALQCIAQTAAQNLLLAPQAKIAQ
                     EIRADITTRIRDRAALTYDAMLTNVRDVLRTLQTGDRLRAVLRKEFRAALIDEFQDTD
                     PVQYDIFWNLFSEGSIPLVFVGDPKQAIYGFRGGDIFTYYGAKEAIAASHQHSLETNF
                     RSEANLVAAINEFFADPVNGRTFQNEHIAYSGQLKANDVPAAKALLVDGARDDQPFHI
                     WTLDDPQQKDWSSPVAREIVRLLSDPKTTIGGSPVRPNQIAVLVNTHKEAAAVESAIK
                     GMSVNAVRQATDNIFNSPEAANLSLLMAAVLEPGKGRAIRNALASGLLPCTHDDLRHY
                     NAASDNLSPGIQPPAAASRPFEEWSEFFHGAGERWNQYSFMEAIQYLFSQLEVLPHLA
                     ASPDGTERIADLRHLIEIIHQAARSKKLGPIALTRWFNRQRKSEFRDKDGDDDATARV
                     SDDSDAVQIMTHFKSKGLQFPIVFAPTLWKLQTRSVRADDATCKYHENNQLILDLDTA
                     SAAGKEKAKTEAHQENIRKIYVALTRAINRVYLFAADGNPKPEQYALSWLLEHVSGTT
                     PHILRSPVPTDLPTAPWQDPTPPPAPESLQPRALNNPVDKSHGHASFSSLMPHPAAAA
                     VITTSAAEAAADAPVRDIDQATEAIPPTAEIVIDPGSILAIPGGAKVGNCWHDIFEHI
                     DFTHADSNPDAINEIIEITLNRYRLLPKPPANASAELRDLMTKRRAAVRTMVFNTLHT
                     PLQPLGDAPTFALKDIPIANRRSEMEFNFSLQDDNQHRLNGIGEILDKQWNSTARNEE
                     FIARLLRSEAAIPRGFMTGFMDLVFHHNNRFYIVDWKSNRLDGKIESFGPAGLAAEMS
                     RNAYYLQYLIYSVALHGFLAARLKNYSFDDHYGGAFYLFVRGMDGKTDSGVFHDKPSR
                     ELIESLAKFLGGTP"
     gene            complement(6585..9983)
                     /gene="recC"
                     /locus_tag="PKI64_06730"
     CDS             complement(6585..9983)
                     /gene="recC"
                     /locus_tag="PKI64_06730"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOE00739.1"
                     /translation="MQLNIAWGSSLERMADDLFRRLGECRVGTAEIFAKRDCIVVPNR
                     IQQAWLQHHFLFDMPRGATPHVLANCDFPPFNLFINDWLNRISPAEQPGRPDPEKHPF
                     SVKSTRWRIFNFLLNGDLSGDFAPLLQYVQSRGSGSRDSRKCFKLAGRLAALFDQYVT
                     YRPAMMLEWSKSTPASLPPELHWEPALWRQITSGAQNYTYLDLYQRMIEHLGECSIEN
                     QYRRIFVFNPSLMPPVHLAFFKLLGDILPVHLYLFNPAPAHDWFDRESLRQRIQDAAR
                     TDALFDLPGDPDDLLNLKHPLLDAYGRGARDMAGITLDFGDGQIVETATPAYSNSLLH
                     TLQQSIIECDASMDTPPLPPDGSIQIHKCHGKMREVEILRDQLLDCFNNDPTLQPRHI
                     QVQVPDLNDYAPYIEAVFSTPNRDARDTIPFVIADSVSTGESQAAEAFRQLLALSDSR
                     FTAPEILDLLRYPGIARRFHFSPGDVDNAAAWIQKAGIRWGRNRHHRREVVADFTEDT
                     TWQVGFDRLFLGYALGSKDDHFPEFQISPSDTVEGGDAILLGHLARFYNALTDFAKFS
                     SSEHSLAEWADRLDLALNDFFLSDNETYLHIAVLRKAVGLLRTTAAASGFIGKVPIAI
                     IRDFLAGHFNETEGGSELHRNAVVFSSLRPGSTAPRRVQALLGMGDSLFPRVNDRPAY
                     DLLRDTRKMGDRSSTIEDRQAFLEVILNAREILLIFYPAFSEEDNTASIESVAVRELA
                     EYINRRFQSKEGPSPLQTFTHRLQAHNPAYFTASSPLFSYSSTAGKTAQAILQPPAPA
                     PAAAAAPAAAPAPSSPTIHVGLHELISFFKNPAEYYFTKILKASLSTGDDIMLEDTEP
                     FASDTLQNWQVRKRLIDSLAAGEDDSARQRILAELTANGNAPFGPAGQKWFAELRDEA
                     EALINQSPGNHLPILKDILVAQKSLTRTEFRVSIPVRDIQVELSGFDRLHPACGIVHF
                     QPSSAQPHKHIGTWLTHLLVNADPSSSRPCSCSVMRKKAELDPLHFAPFKSPADAQNI
                     LAAYLEIFLYQSAPPPRYTPDTAWAFVEQFHGKGKTQDVSLREAKGKWENPYGGSSFQ
                     GEANPYYLQLYGADGPFEPFDKFRSAAEKIILPMWRHLNPEDPA"
     gene            10122..11534
                     /gene="leuC"
                     /locus_tag="PKI64_06735"
     CDS             10122..11534
                     /gene="leuC"
                     /locus_tag="PKI64_06735"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOE00740.1"
                     /translation="MSTTLYDKVWSRHVVREETNGSTLLYIDRQLVHEVTSPQAFDGL
                     RLAGRKVRQPRLNVATIDHNVPTVDPLNIMDRIAKAQIDTLKKNCAEFGLKLYGIGDP
                     STGIVHIMAPELGLTLPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
                     RSRSMRATFTGRPGKWVTAKDFILRLIREIGTAGGTGCVLEYAGEAIRALNMSGRMTI
                     CNMSIEAGARAGLIAPDDTTFEYIASGDKPYAPRGAALDKSIAFWKTLRSDDDATFDR
                     EITIDTSTLVPQISWGTSPGMVVDIDGVVPGPHDVPTVQPSDVEKALAYMDLKPGTPL
                     TSIPVDVVFIGSCTNGRIEDLREAAGVLKGRRVAPKVQTLVVPGSARVKAQAEAEGLD
                     QIFLAAGAEWRNPGCSMCLAMNPDRLKPGQRAASTSNRNFEGRQGQGGRTHLVSPVMA
                     AAAAVAGHFVDVRTWLEGGN"
     gene            11536..12168
                     /gene="leuD"
                     /locus_tag="PKI64_06740"
     CDS             11536..12168
                     /gene="leuD"
                     /locus_tag="PKI64_06740"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOE00741.1"
                     /translation="MDAFKTFRGVVATLERANVDTDQIIPKQFLKSIERTGFGPALFF
                     DWRYLPDGQPDPAFELNAPERAGASVLVTGNNFGCGSSREHAVWAVQQYGFKAVIAPW
                     RGEGDKRIPGFADIFKNNSYGNGLLVIELASADVDWIFSAAKGRKLEATVDLAAQTVI
                     VHADPDRVFPFEITPDVKEKLLSGLDAIGLTLTHEADITAFEAKHDVQMV"
     gene            12208..12948
                     /locus_tag="PKI64_06745"
     CDS             12208..12948
                     /locus_tag="PKI64_06745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE00742.1"
                     /translation="MNLILLEPGEIQADGTAFLSDDRARHIREVLRATPGASLRIGMV
                     DGPLGRVKVVALVPAVQLECHLAGATPPRPPVDLLLAMPRPKVMKRLWPVLAALGVGR
                     ILITNAWKTERNYFDTHVLAADFVRAQLIAGLQQAGDTRLPEVSVYRQFKKAVAALTD
                     GGPYAAQLVAHPGPAAFPHAALAALPAASRIVLAVGPEGGWVPYELEWLAARGFAAVS
                     WGPRPLRTDTACTVLLGLIHAARAGSPA"
     gene            13042..14175
                     /locus_tag="PKI64_06750"
     CDS             13042..14175
                     /locus_tag="PKI64_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOE00743.1"
                     /translation="MKIWICVLLMMGLVVGEARAEMPADLAADGQAALARGAAWLAAQ
                     QQADGHWGSPETPALTALAMWALHRANPAEYQDAIARAMVYVLTSVHEDGSIWRAPSM
                     ERRGGGLANYNTAVCMSALHALGRPELVPVIQKARTYLAKMQYLDDGIYRGGMGYDAE
                     TGRAYADLSNSYMAYEAMRLTQDVEDLRAAGDPKADLDWGAVQEFLAQVQNLPGVNTN
                     SYVSDDADSRGGFAYTPGAGAANTNAEGKVTLRSYGSMTYAGLLALIYAQVDETDVRV
                     QAAKDWAERHWSLDENPGMGQEGLYYFFNVLTKSLKAAGDATLTLPDGRTVVWREEVA
                     RKLVALQKPDGHWVNDNNRWWEAEPNLVTSYVLLSLASALTPQ"
BASE COUNT         2633 a         3625 c         5172 g         2924 t
ORIGIN      
        1 ggagacaacc atgaatgatg caacaaacaa cgtgacacca attccttcat ccaatcctcc
       61 ttcgggggcc ggtgagaaaa gcaaagtcgc cgctggcgtt ctgggtattt tgttgggcgc
      121 gcttggagtt cataagtttt atctgggcta tacccaagcc ggcctcatta tgttgctggt
      181 ttccattgtc ggcggcatct tcacctgcgg catggccagc agcgtaatcg gcatcatcgg
      241 gctcgtggaa gggattctgt atttgaccaa gtccgacgaa gaattcacgg ccacatacgt
      301 gcaaaataaa aagccctggt tctagtcggg gcattcacac acacacaagt ctgcctcgga
      361 tgcgaggcca agaggaggcc ggctcatggg tgacacaccg aacctgaacc cgcaaaatat
      421 tcccaactat ctctggcaat ccatcgtggt gacggttctt tgctgcttgc cattgggaat
      481 ccccgccatc atctacgcca gcaaggtgaa caatgccctg atgcagggga atcagcaggc
      541 cgcgtggcag gcatccaata ctgcaaaaat gtggtgcatt atcgctttgg tggccgggct
      601 aatgggtggt tttctttatt tcatggtgtt tggcttggca ctcatttttg gttaggcgtt
      661 atgacgccgg ggcggtggat ggctggcctc ctgttggcgg ggctggtgct gctggcggtg
      721 ttgccgccta atgtgggttg cttccccccg tgcccggttc acacgaccac cggactatat
      781 tgttcaggtt gcggcagcct acgtgccatt cgcagtttgt tatccggcga tgtcctgcag
      841 gcttggcact ataatccgat tctgctgtgt tgtatcccca tcatcttggt aggggtactg
      901 tcagaagtag tacacacaaa gcgcttatct ctgactcgaa ttcgcccatt tttattatgg
      961 gtttggctga gcgtcattct ggcattcggc gttctgcgca atattccggc ttttgactat
     1021 ctgcagccca ccgcattgtc ggcgaaaacc atccatgccg gcgcactgac ttggccgcga
     1081 taatacttgc cagtagctag gcaactggcg cggaggggtg gtccaatttt tgggcgaggc
     1141 ccatggcgcg ggtggtggcg gtttggacag cggattggaa ggtctctttg gtggtccaga
     1201 ggtcaacgcc ggtttcggtg cgggtgatgg cggtataaat cagctcgcga gtgaggaggg
     1261 gtgtagcacg gtcggggagg atgacggcga catggttgaa gccggagccc tgcgctttgt
     1321 ggacagtcat ggcgaaggcg gtttcgtgtt caggcaaaag gtggcaggga actgggtggg
     1381 ggcggccctc gaagaaggcg gcgggctggt tgttgcggtc ggggtcgggg aggatgatgc
     1441 cgacatcgcc gttgaagagg ttggtttcgt agtcattggt ggtaatgagg atgacgcggt
     1501 ggtcgtagaa ttcgccgcgg cggttgggga agaggatgtc ttcgatggcg cggttgatgt
     1561 tgttgacgcc ggcggggccg cggcgcagag cgcacaaaac gcggaatttg gccagggtcg
     1621 caaaggcatt ggcgggggtg gcggcatttt tgaagtcggc gaagcgttct ttgacgaggt
     1681 tggtgaactc tctcgggagg tcgccgtagc ggatttgggt ggcgtcgtgc agggcgattt
     1741 ttttgccggg ggcgccaagg gtggggtcgg aggcgatttg ccaggcgagg ttggcgtttt
     1801 gttggcggac ggcttggctg aggggggtga cggcggaggt ggcgtcgaac cgtttgcttt
     1861 cggtgagctc gacgacagat ttttgctgca gcagggttga gtcgcagagt tccgccatga
     1921 cgctgccggg gtccacggag gcaagttggt ttttgtcgcc aagcagaatg agccgggcgg
     1981 aaagggggat ggcatcgagg agcttcgcca ttttgggtag gtcaatcatg ctggcttcgt
     2041 ccacgatgaa agcatcggcg ctgataggat tgtcgcggtt atggcggaag tagggggagt
     2101 tggggagata gccgagggcg cggtcaatgg tagcggcggc gggaatttcc attcggtcgc
     2161 gggctgcctc cgggaggccg ctgcgcaggc tttcatcgag gcgcgcggcg gctttgccgg
     2221 tgggggcgag gaggcggacg cggagccgga ggtgttcggg cgcgagtttg gcgatctgct
     2281 caaggagcac ggcggcgatg gtggttttgc cggtgccggg gccgccggtg atgatggtaa
     2341 attgtcgttc aagagcggca aggacggcat cctgctgacc tttcgccaag ggggaaccgg
     2401 agggttggag tttgtaggtg cggatggcgt tttggatttc ttccgggacg gcgacggggt
     2461 cgagttgggc gaagacgcgg agtttttcag cgacgagtct ttcgtagttc cagaatcggc
     2521 ggagatagag ccgagtttct tcaaggacga agggggtggg ggtggagccg tcgctgacga
     2581 gaccggggag gttttcgagc agggggagaa cgatggccgg agacgggagg ctgttgagtg
     2641 tttggttggc gagtgtcgtg tcatcgcccg gtccggcgga atcttcggcg ggttcggcgg
     2701 gaacgttgcc ttgtaactgc caccaggcgt cctggccgag gtcgaggcag acgtggccgt
     2761 cgccggtgac gcggctggct agggtgagga tgcactggac gataggatcg ggctcgatga
     2821 agccggggtg agcggcggcc gtgcggatga gagattcgat taagaacaga tcaagatcac
     2881 ggatgaggtt ggcgtcgcgg agctgctgcg gcgtgcaggt gcggggctgg ttcatggcgt
     2941 gcctccgaga aacttggcga gggattcaat cagttcgcgc gagggtttgt cgtggaagac
     3001 gcccgaatct gttttgccat ccatgccgcg gacaaagagg tagaaggcgc cgccgtagtg
     3061 gtcgtcgaag gaatagtttt tgaggcgggc ggcgaggaat ccgtgcagcg cgacagagta
     3121 gatgagatat tggaggtagt aggcgttgcg ggacatttcg gcggcgaggc cggcggggcc
     3181 gaagctttcg attttgccgt cgaggcggtt ggatttccag tcaacgatgt agaagcggtt
     3241 attgtgatgg aagacgaggt ccatgaagcc ggtcatgaag ccgcgcggga tcgcggcctc
     3301 gctgcgcagg aggcgggcga tgaattcttc attgcgcgcg gtggaattcc actgtttatc
     3361 caggatttcg ccgataccgt tgagtcggtg ttggttgtca tcctgaagcg agaaattgaa
     3421 ctccatctcg gagcggcggt tggcaatggg gatgtctttc agagcgaagg tgggagcgtc
     3481 gccgaggggc tgcaaggggg tatgcagcgt gttgaacacc atggtgcgaa ccgcggcgcg
     3541 gcgtttggtc atcaggtcgc gtaattccgc agaggcgttg gccggtggtt tggggaggag
     3601 gcggtagcgg ttgagggtga tttcaataat ctcgttgatg gcatcgggat ttgagtcggc
     3661 gtgagtgaag tcaatgtgtt caaaaatatc gtgccagcag tttccgactt tggcgccgcc
     3721 gggaatggcg aggatcgagc cgggatcaat gacgatttct gcggtgggcg gaatggcctc
     3781 ggtggcctga tcaatatcgc gcacaggtgc atcggcggcg gcttcggcag ccgaggttgt
     3841 tatgacggcg gcggcggcgg gatgcggcat gagggaggag aagctggcgt ggccatgcga
     3901 cttgtccacg gggttgttca aggcgcgcgg ttgcagggac tccggagcag gcggcggggt
     3961 aggatcctgc cacggagcgg tgggcaggtc ggtagggacg ggactgcgga gaatgtgggg
     4021 cgtagtgccg gaaacgtgtt ccagcagcca tgacaaggca tattgttcgg gcttgggatt
     4081 gccatctgcg gcaaaaagat agacccggtt gatggcgcgg gtgagcgcga catagatttt
     4141 gcggatgttt tcctgatggg cctccgtttt ggctttttcc ttgcccgcgg cggaggcggt
     4201 gtccaagtcc aggatgagct gattgttttc gtggtatttg caggtggcat cgtctgccct
     4261 gaccgagcgg gtttggagtt tccacagcgt gggcgcaaag acgatgggga actggaggcc
     4321 cttgcttttg aagtgggtca tgatctgcac ggcgtcgctg tcgtcagaga cgcgcgcggt
     4381 ggcgtcgtcg tcgccgtcct tatcgcggaa ttccgatttg cgttgccggt tgaaccagcg
     4441 ggtgagggcg atggggccga gttttttgga gcgtgccgcc tgatggatga tctcgatgag
     4501 gtggcggaga tcggcgatgc gttcggtgcc gtcgggggag gcggcaagat ggggcagcac
     4561 ttccagttgg gagaagagat attggatggc ttccatgaag gaatactggt tccagcgctc
     4621 gccggcgccg tggaagaact cgctccattc ttcaaacggt cgcgaagcgg cggccggcgg
     4681 ctggatgccc ggagagaggt tgtcgctggc ggcgttatag tggcggaggt cgtcgtgggt
     4741 gcagggcagg aggccggagg cgagggcgtt gcggatggcg cgccctttgc cgggttcgag
     4801 cacggccgcc atgaggagtg aaagattggc ggcttcgggg ctgttgaaaa tgttgtccgt
     4861 ggcctgccgg acggcgttga cgctcatccc tttgatggcg gactccacag ccgccgcctc
     4921 tttgtgcgtg ttgacgagaa cggcgatttg gttggggcgg acgggagagc cgccgatggt
     4981 ggttttgggg tcgctcagca ggcggacgat ttcgcgggcg acggggctgg accagtcttt
     5041 ctgctgcggg tcgtccaagg tccagatgtg gaagggttgg tcatcgcggg cgccatcaac
     5101 cagcagcgcc tttgcggcgg ggacatcatt tgccttgagt tggccggagt aggcgatgtg
     5161 ttcattctgg aaggtgcggc cgttgactgg gtcggcaaag aattcgttga tggcggccac
     5221 cagattggct tcggagcgga agttggtttc gagggagtgc tgatggctgg cggcaattgc
     5281 ttccttcgcg ccgtaatacg tgaagatgtc gccgccgcgg aagccgtaga tggcttgttt
     5341 ggggtcgccg acgaaaacca gcgggatgct gccttcggaa aagagattcc agaaaatatc
     5401 gtactggacg gggtcggtgt cttgaaattc gtcaatgaga gcggcgcgga attccttgcg
     5461 gaggacagcg cggaggcggt cgccggtttg aagggtgcgg aggacatcgc ggacattggt
     5521 aagcatggcg tcgtaggtga gcgcggcacg gtcgcggatg cgggtggtaa tgtcggcgcg
     5581 gatttcttga gctattttgg cttgtggagc caataacaag ttctgggcag cagtttgggc
     5641 gatacattgc agggccagca caaaaggttt ggcattgccg gtggttggcg gagctgacgc
     5701 tgtattcacg atggttaaaa aagactcgcc gagttcatga cgcggggcgg gcaattccct
     5761 ttgtgcagcc gcccaagcat cccattcagc cgggttctgc ttgatggagt cgaagacggg
     5821 agaagcatca acagtgtcat ctacccgggt gattattgcc ttggatttcc aattgaaatt
     5881 accgcgcatg tttttgaagc cagtaaaaac ggtcttgcag gcggaggtgg catcttggaa
     5941 ggcaggtgta tcgggcagcg cggcggctct gatggtggcg gtggggtgtt tgtagagttc
     6001 ggttacgagc tggataagtt ggccgatctt ctcaaacggg ttgggtgagt cgggggaggg
     6061 ggtgtaggcg tttttgcgcc accagtcctg gcaggcctcg cggatgatgt gggaggcttc
     6121 aggcatgagt tcggcgtcgg ggtcatggcc gcattcgaag gcgtagtgtt tgaggacgcg
     6181 ctggcagaag ccgtggatgg tgatgatggc ggcgctgtcg aagtccatga tggcgagctg
     6241 gacgcgctgc ttgagcagtt ggatttcctc atccgttttg tgggggacga gttgaagggc
     6301 gtcgtggatg cgctgctgtt ccggggtgag cgcgccggcg gggggggctt ccagataata
     6361 acggcaatcg aggaggacgt tgcggaggcg ttcacggagt tcgcaggtgg cggcgttggt
     6421 gaaggtgacc acgaggattt cgcggacgga gaggtttttc tcgaggatga ggcggaggaa
     6481 gacgttctgg atgttgtagg ttttgccggt gccggcgctg gcttcgacaa gctggggcgt
     6541 gtcgaaagcg tgggtcggag aaaagacgag attggaaaag tcgctcatgc cgggtcctcc
     6601 ggattgagat gccgccacat ggggaggatg attttttcgg cggcgcttct aaacttgtcg
     6661 aagggttcaa acgggccgtc ggcgccgtag agctgcaagt agtaggggtt ggcctcgcct
     6721 tggaaggatg agccgccgta tggattttcc catttccctt tagcctccct caatgagacg
     6781 tcttgtgttt ttcccttgcc gtgaaactgc tcgacaaaag cccaggcggt gtccggggta
     6841 taacgtgggg gcggggcgga ttgatagagg aagatttcga ggtaggcggc gaggatgttt
     6901 tgggcgtcgg cgggggactt gaagggggca aagtggagcg ggtcgagttc agctttcttt
     6961 cgcattacgc tgcaggagca gggccgagat gaggaagggt cggcgttgac caagaggtgg
     7021 gtgagccagg tcccaatgtg cttgtggggt tgggcggatg agggttggaa gtggacgatg
     7081 ccacaggcgg gatgcaggcg gtcgaagccg gagagttcca cctggatgtc gcgcacaggg
     7141 atgctgacgc ggaattcggt tcgagtgagc gacttttgtg caacgagaat atctttgagg
     7201 attggcaggt ggttgccggg ggattggttg atgagggctt cggcttcatc gcgcagttca
     7261 gcgaaccatt tttgtccggc ggggccgaaa ggggcgttgc cgttggcggt gagctcggcg
     7321 aggatacgct ggcgggcgga gtcgtcttcg ccggcggcaa gcgagtcaat gagccgcttg
     7381 cgaacctgcc agttttgcag agtatcggat gcaaaaggct cggtgtcttc gagcatgatg
     7441 tcgtcgccag tggataacga ggccttgagg atttttgtga agtagtactc ggcggggttt
     7501 ttgaagaagc tgatcagctc gtgcaggccg acgtggatgg tgggtgaaga tggagcgggc
     7561 gcggcggcag gggcagccgc ggcggcgggg gccggcgccg gcggctggag aatggcttgg
     7621 gcggttttgc cggcggtgga ggagtaggaa aagagcgggg agctggcagt gaaataggcc
     7681 gggttgtggg cttgcaggcg gtgagtgaag gtttggaggg gtgatgggcc ttctttggac
     7741 tgaaagcggc ggttgatgta ttccgccagc tcgcggacgg cgacggattc gatggaggcg
     7801 gtgttgtctt cctcggagaa agcgggatag aagatgagga gtatttcgcg ggcgttgagg
     7861 atgacttcga ggaaggcctg acggtcttcg atggtggatg agcggtcgcc cattttgcgg
     7921 gtgtcgcgga gaaggtcgta ggcagggcgg tcgttgacgc gcgggaagag ggagtcgccc
     7981 atgccaagca gggcctgcac acggcggggg gcggtgctgc cggggcggag ggaactgaag
     8041 acgacggcgt tgcggtgcag ctcggagccg ccttcggtct cgttgaagtg gccggcgagg
     8101 aaatcgcgga tgatggcgat ggggactttt ccgataaagc cggaagcggc agcggttgtg
     8161 cgcaggaggc caacggcttt tctgaggacg gcgatgtgga gataggtttc gttatcgctg
     8221 aggaagaagt cgttgagggc gaggtcgagg cggtccgccc attcggcgag agagtgttcg
     8281 gaggaggaga atttggcaaa gtcggtcagg gcattgtaga agcgggcgag atggccgagg
     8341 aggattgcgt cgccgccttc gacggtgtca ctgggagaaa tttggaattc ggggaaatgg
     8401 tcgtccttgg aaccgagagc gtacccgagg aagaggcgat cgaagccgac ttgccaagtg
     8461 gtatcttcgg tgaaatccgc cacgacttca cgccggtgat gccggttgcg gccccagcgg
     8521 atgccggctt tttgaatcca cgcggcggcg ttatccacgt cgccggggga gaagtggaaa
     8581 cggcgggcga tgccggggta gcggaggagg tcgaggattt cgggagcggt gaagcggcta
     8641 tcgctgagag caaggagctg gcggaaggcc tcggcggcct ggctttcgcc ggtggaaacg
     8701 ctgtcggcga tgacgaaggg gatggtgtcg cgagcatcgc ggttgggagt agagaagacg
     8761 gcttcgatgt aaggcgcgta atcgttgagg tcggggactt gcacctggat gtggcggggt
     8821 tgcagcgtag gatcgttgtt gaagcagtcg agaagttgat cgcgcaggat ttcgacttca
     8881 cgcattttgc cgtggcactt gtggatttgg atggaaccgt cgggggggag agggggcgtg
     8941 tccatggagg catcgcactc gatgatggat tgctggaggg tatgcaggag agagttggag
     9001 taggcgggag tggcagtttc aacgatttgt ccatcgccga aatcgagagt gatgcccgcc
     9061 atgtcgcgtg cgccgcggcc gtaggcatca aggagggggt gtttgaggtt gaggagatcg
     9121 tcggggtctc cgggcaggtc gaagagagcg tcggtgcggg ctgcgtcttg gattcgctgg
     9181 cggagggatt cgcggtcgaa ccagtcgtgt gcaggagcgg ggttgaagag gtagaggtga
     9241 acggggagga tatcgccaag gagtttgaag aaggccaggt ggacgggggg catgagggag
     9301 gggttgaaga caaagatgcg gcggtattgg ttttcgatgc tgcattcgcc gaggtgctca
     9361 atcatgcgtt gatagagatc gaggtaggta taattttggg cgccggaagt gatttgccgc
     9421 catagcgcag gttcccagtg gagttcaggc gggagagaag cgggggtgga cttgctccat
     9481 tcgagcatca tggcggggcg gtaggtgacg tactgatcga agagcgcggc gagacgcccg
     9541 gccagcttga agcatttacg ggagtcgcgg gaaccggagc cgcgggactg gacatattgc
     9601 aagagggggg cgaagtcgcc ggagaggtcg ccgttgagca ggaaattaaa aatgcgccag
     9661 cgggtgcttt tgacggagaa ggggtgcttt tcgggatcgg ggcggccggg ctgctcggcg
     9721 ggggagatgc ggttgagcca gtcgttgatg aagaggttga aaggggggaa gtcgcagttg
     9781 gccagcacgt gcggcgtggc gccccggggc atgtcgaaga gaaagtggtg ttggagccac
     9841 gcctgctgaa tacggttggg gacaacaata caatcgcgtt tggcgaagat ttccgccgtc
     9901 ccaacgcggc actcgcccaa acggcgaaag agatcatccg ccatccgctc caagctgctg
     9961 ccccacgcga tattcagttg catatcaatg agcctttccc accttgccat gtaccatcct
    10021 agcacaaaaa aacatcattt tgaaggctaa tctgggttgg tgacaaaacg tgtccgaagc
    10081 ttgcgccgtt ggggggaggg ccgtatagtc cggcgttacg tatgagcaca actctctatg
    10141 acaaagtctg gtcgcgccac gtcgtgcgcg aggaaaccaa cggatccacc ctgctataca
    10201 ttgaccggca gttggtccac gaagtcacca gcccgcaggc gtttgacggt ctgcgcctcg
    10261 ccggccgcaa ggtgcgccag ccccggctga acgtggccac cattgaccac aacgtgccca
    10321 ccgtggaccc gctcaacatc atggaccgga ttgccaaggc ccagattgac acgctcaaga
    10381 aaaactgcgc cgagttcggc ctgaaactct acggcatcgg tgatccctcc acgggcatcg
    10441 ttcacatcat ggcgccggag ctggggctga ccctgccggg gttgacgatt gtctgcggtg
    10501 actcgcacac ggccacccac ggcgcgttcg gcgcgctggc gttcggcatc ggcaccagcg
    10561 aggtggaaca cgtactggcc acgcaaaccc tctggcagcg gcgtagccgc tccatgcggg
    10621 ccaccttcac cgggcgcccc ggcaaatggg tcaccgccaa ggatttcatt ctgcgcctga
    10681 ttcgcgaaat cggcaccgcg ggcggcaccg gctgtgtgtt ggaatatgcc ggcgaggcca
    10741 tccgcgcgct gaacatgtcc gggcgcatga ccatctgcaa catgtccatc gaagccggcg
    10801 cgcgcgccgg cctgatcgcg ccggacgaca ccacgttcga atacatcgcc agcggcgaca
    10861 aaccctacgc cccgcgcggc gcggcgctgg acaaatccat cgcgttttgg aaaacgttgc
    10921 gcagcgatga cgacgctact ttcgaccgcg aaatcaccat tgataccagc acgctcgtgc
    10981 cgcaaatcag ttggggcacc agcccgggca tggtggtgga tattgatggc gtggtgcccg
    11041 gcccgcacga cgtgcccacc gtccagccca gcgatgtcga aaaagcgttg gcctatatgg
    11101 acctcaaacc cggcacgccg ctgacctcca tcccggtgga tgtggtgttc attggcagtt
    11161 gcaccaacgg gcgcattgaa gatttgcgtg aagccgcggg cgtgctcaaa ggccgccggg
    11221 tggcgcccaa ggtgcaaacg ctcgtggtgc ccggctcggc gcgcgtcaag gcgcaggccg
    11281 aagccgaagg gctggaccaa atcttccttg ccgccggcgc ggagtggcgc aaccccggct
    11341 gcagcatgtg cttggccatg aaccccgacc ggctcaaacc cggccagcgt gccgcgtcca
    11401 cctcgaaccg caactttgaa ggccgccagg ggcagggcgg gcggacgcat ctggtcagcc
    11461 cggtcatggc cgccgccgcc gcggtggccg gtcactttgt ggatgtacgc acttggctcg
    11521 aaggaggcaa ctaacatgga tgcattcaaa acttttcgcg gtgtggttgc cacgttggaa
    11581 cgcgccaacg tggatacgga tcaaatcatt cccaagcagt ttttgaagtc catcgagcgt
    11641 accggctttg gcccggcgtt gttcttcgac tggcgctacc tgcccgacgg ccaacccgat
    11701 cctgcttttg aactcaacgc accggaacgc gccggcgcct ccgtgctggt gacgggcaac
    11761 aactttggct gcggttccag ccgggaacac gccgtctggg ccgtgcagca atacggcttc
    11821 aaagccgtca ttgccccatg gcgcggcgag ggcgacaaac gcatccccgg ctttgccgac
    11881 atcttcaaaa acaacagcta cggcaacggc ctcctcgtga ttgaactggc cagcgccgac
    11941 gtggattgga ttttcagcgc cgccaaagga cgcaagctgg aagccactgt ggacctcgcg
    12001 gcgcaaaccg tcatcgttca cgccgatccc gaccgcgttt tcccgttcga gatcacgccc
    12061 gacgtgaaag aaaaattgct cagtgggctg gatgccatcg gcctcacgtt gacccacgaa
    12121 gccgacatca cggccttcga ggccaagcac gacgtccaaa tggtgtagcc cgccgccgcg
    12181 cgggccttgc tctccgggcg gttccccatg aacctgattt tgcttgagcc cggcgagatt
    12241 caagcggacg gcacggcgtt tttatccgat gaccgtgcgc gccacatccg cgaagtgctg
    12301 cgcgccacgc ccggcgcctc cctgcgcatc gggatggtgg atggcccgct cggtcgcgtc
    12361 aaggtagtgg cgctggtccc ggcggtgcag ttggaatgcc acttggccgg cgccacgccg
    12421 ccgcgtccgc cggtggatct cctcttggcc atgccgcgcc ccaaagtgat gaaacgcctg
    12481 tggcccgtgc tggccgcgct gggcgtcggg cgcattctca tcaccaacgc gtggaaaacc
    12541 gaacgcaact atttcgacac ccacgtcctc gccgccgatt ttgtccgcgc ccaactgatc
    12601 gccggcttgc agcaggcggg agatacccgg ctgccggaag tcagcgttta tcgccaattc
    12661 aaaaaagcgg tcgcggcttt gaccgatggt ggcccctatg ccgcgcaact ggtggctcat
    12721 cccggccccg ccgcattccc ccatgccgct ttggctgcgc tccctgccgc cagccgcatc
    12781 gtgctggccg tgggccccga aggcggctgg gtgccctacg aactggaatg gctggccgcg
    12841 cgcggctttg cggccgtctc gtgggggccg cgcccgctgc gcaccgatac cgcctgcacc
    12901 gtgctgctcg gcctgatcca tgcggcgcgc gccggctctc ccgcgtgacg ctttcccaaa
    12961 aatagcgtac actgattttc cgtgtggcga acccgcgcgg gcgctgcggc gtttatacag
    13021 gatgatggaa aggacaaacg gatgaaaatt tggatttgtg ttttgctgat gatgggactg
    13081 gtggtgggcg aagcccgtgc cgagatgccg gcggacctgg ccgcggatgg ccaggcggcc
    13141 ctcgcccgtg gcgcggcctg gttggccgcg caacaacagg cggatggcca ctggggcagc
    13201 cccgaaacgc ccgcgttgac ggcgctggcc atgtgggcgc tgcaccgcgc caacccggcg
    13261 gaatatcaag acgccatcgc gcgggccatg gtgtatgtgt tgaccagcgt gcacgaagat
    13321 ggctccattt ggcgcgcgcc ttccatggaa cggcgcggcg gcgggctggc caactacaac
    13381 accgcggtct gcatgtccgc gctccatgcg ctgggccgcc cggagctggt acccgtgatc
    13441 caaaaggcac gcacctacct cgccaaaatg cagtatctcg atgacggcat ctaccgtggc
    13501 ggcatgggct acgatgccga gaccggccgc gcgtatgccg acctctccaa ctcctacatg
    13561 gcctacgaag ccatgcgcct gacgcaggat gtcgaagacc tgcgcgcggc gggcgatccc
    13621 aaggccgatt tggattgggg cgcggtgcag gaatttctgg cgcaggtgca aaacctgccc
    13681 ggcgtgaaca ccaattccta cgtcagcgac gacgccgact cgcgcggcgg cttcgcctac
    13741 accccgggcg cgggcgccgc gaacaccaac gccgaaggca aagtcacgct gcgctcctac
    13801 ggcagcatga cttacgccgg cctgctcgcg ctgatctacg cccaagtgga tgaaaccgac
    13861 gtgcgcgtgc aagccgccaa ggattgggcc gagcgccatt ggtcgctcga cgaaaacccc
    13921 ggcatggggc aggagggcct ctattacttc ttcaacgtgc tgaccaagag cctgaaggcc
    13981 gccggcgatg ccaccctcac gctccccgac ggccgcaccg tggtctggcg cgaagaagtc
    14041 gcgcgcaaac tggtggccct gcaaaagccc gacggccact gggtcaacga caacaaccgc
    14101 tggtgggaag ccgagcccaa cctcgtcacc tcctacgtcc tgctgtccct cgcctccgcc
    14161 ctcacccccc aataggggat agcccgataa tttattgttc tggggttggc gcgttgccga
    14221 acgccgcact tttccacgct gtggaaaatt attcatcccg cgtgggcggg attttccacg
    14281 gtgtggaact tttgccgccg atttttccac agataggatg agaaggcaaa agaggatcgg
    14341 tttcctttgg gcgt
//
LOCUS       DAOPNP010000060        14242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5008_length_14242_cov_8.301050, whole genome shotgun sequence.
ACCESSION   DAOPNP010000060 DAOPNP010000000
VERSION     DAOPNP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_14242_cov_8.301050"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PKI64_06755"
     CDS             complement(<1..1500)
                     /locus_tag="PKI64_06755"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOE00744.1"
                     /translation="MAHFIFITGGVVSSLGKGITAASIGRLLISRGLRIRMLKMDPYL
                     NVDPGSMSPDQHGEVYVTDDGALADLDLGHYERFTGQPTTRQSSVSSGRIYWDVLQKE
                     RRGEYRGHTVQMIPHITDEIKASIRALAAPDVDVVLTEIGGTVGDIEGLPFLEAIRQI
                     GLEEGRERVLYIHMTYLPLIRAAGELKTKPSQQSVAKLREIGIVPDVLVCRSEQPLTQ
                     DIRRKLSLFCNVPLHAVIEELDVRHTIHEVPLVLAEQGLDEIILVHFRLPRPPAQLAD
                     WRAMVAAITQPRGTVRIGVVGQRAELPDTHKSLHEALRHGGGAHQQSVEIIPLPAGDF
                     TAGKAPARLPEINALLIPDGGDADGLVGRLAAVRHARETGLPFLGIGRGFHCAVIEYA
                     RHVCGLTEANDTDTAPDTPHPVLHAAPHGEQLGAKPCALKPDSLLAKIYGATRISERH
                     RHALELNPAYRPQLEAAGMVCSGTDPAGHDVQVIELPAHPWFVAVLFHPE"
     gene            complement(1518..2234)
                     /locus_tag="PKI64_06760"
     CDS             complement(1518..2234)
                     /locus_tag="PKI64_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00745.1"
                     /translation="MELALPDPITNYNRQIARQRYLRRAEQVLGHHPGFNLAYWRRIV
                     RASDRVDRLGRHWGWSADQVKLTKALLITRMACVFIDHLIQRMKQAGIIPPHPDEPHL
                     EWERQYRNFLLMFLRTPEIKDRREIVKNMQRLHRYTTPPVSLLEFRQRHWRWFAALMP
                     SLLASPNPNTLFIRRQLHLDGPPVAAARRICFTAALYSDIQERAYRQQWMYAYSDCML
                     RLYDRLRATPQVLAAIIALD"
     gene            complement(2250..2552)
                     /locus_tag="PKI64_06765"
     CDS             complement(2250..2552)
                     /locus_tag="PKI64_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00746.1"
                     /translation="MKNEIEDNFGRALQNLVVHLIKNAKKIPPPVLQGALDFENFAWP
                     PLPDGTKRARLREIAGLTAAPSDIHQHFEAYPHKFSKGSYARYLTALRLYQEQLGA"
     gene            complement(2622..4151)
                     /locus_tag="PKI64_06770"
     CDS             complement(2622..4151)
                     /locus_tag="PKI64_06770"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HOE00747.1"
                     /translation="MQAAEAQLKVDVLVVGAGPAGLACAIRLARAGKGQRQIMVLEKS
                     AQLGGHLLSGAVMRPQALQHLLMPDEYAALPIGPLVTRDTFHALLPKGAIRVPFVPPK
                     MRMRGLPLVSLAQLGSALGDIATKLGVEILTSQTADALLWEGDRVSGVRTLDDPILAD
                     TTVLAEGPAGLLTKAVLARHPQLAGPNLQTYALGIKEIVEVPANPALLGTVAHTFGYP
                     LGINIYGGGFIYHMDETHVALGLAIALDYHDPATYPHDLFRRWKRHPFVQRHIAGGKT
                     VEYGARLVPEGGWHSRLGMAAPGAFIIGDSAGLVDTMELKGIHLAVESGMAAADAIIR
                     GDALRQEDMPSLDGLRRTANYRAAFRASLPIGMAAAGLAWISGGRLPWGRIRQRDERQ
                     CLHEIGHRQPPLPQPDNGPLDLGLDSDLFLARLRYRPDTDHIAIPDADKCRECFQRFA
                     APCTRFCPAGVYEADATRTAIHIRAENCVQCRCCTLKCPYDNIRWETPEHGVGPDYQN
                     V"
     gene            4313..5416
                     /gene="ugpC"
                     /locus_tag="PKI64_06775"
     CDS             4313..5416
                     /gene="ugpC"
                     /locus_tag="PKI64_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOE00748.1"
                     /translation="MAKVVLENVYKIYPGDVTAVHDANLEIQDQEFVVLVGPSGCGKS
                     TTLRMVAGLEDISKGSIYIDGKKVNDIPPKDRDIAMVFQNYALYPHMSVYNNMAFGLK
                     LRKYPKAEIHKRVMEAAEILGITDYLERKPKALSGGQRQRVAVGRAIVRKPKAFLFDE
                     PLSNLDAKMRVQMRAEISKLHNRLKSTMIYVTHDQIEAMTMGDRIVVMKDGWIQQVDT
                     PLNIYNKPANQFVAGFIGSPPMNFFKGKIVREGNAVAFAEGRFTVALAGAVADKMAAH
                     AGQAVTMGLRPEHIDERSARPDANPASVVQAQVEVVEMMGSEVYLYANTGQSNFTARV
                     DSSCRKQIGDTVEVVFDMSKAHFFAEDDGRALL"
     gene            5426..5563
                     /locus_tag="PKI64_06780"
     CDS             5426..5563
                     /locus_tag="PKI64_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00749.1"
                     /translation="MSLAWVELIQTIAVAVVAYVLLTALALFFKAEAAGRQAPPESPA
                     P"
     gene            5560..6273
                     /locus_tag="PKI64_06785"
     CDS             5560..6273
                     /locus_tag="PKI64_06785"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOE00750.1"
                     /translation="MKLGVNIDHVATLRQQRGTVYPAPLEAAALCEKGGADGITIHLR
                     EDRRHIQDADLFALRKALRVPLNLEMANSPQIVQIALRARPAEVCLVPERRQELTTEG
                     GLDAAGQFRRLKPTIAKLQAAGIVVSLFIEPDPRQLEAAVALGAPVVELHTGKYCDET
                     GAARRAELKRLVAAAKLGHRLGLQVNAGHGLNYDNLPAFLRAVPHLHTLNIGHSIVAR
                     AVMVGMTRAVREMKALLKP"
     gene            6270..7805
                     /locus_tag="PKI64_06790"
     CDS             6270..7805
                     /locus_tag="PKI64_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOE00751.1"
                     /translation="MKFVTAKTLRAAEAKAIALDRSLALSMMDWAGKGLARVVQDVAA
                     QMGRRNAAVRLLAGPGNNGGDAFVAAMSLHEAGLLPEVWLVGPREKLRGSAKYYFDEL
                     QKSGVPWREMVGEKAWQQAAGLTLTPPIHVDALLGTGARGELLGDIRRAVEYLREKQT
                     RSIIVAADVPTGMNPDTGEAGPSCVTADFTVSMCMPKIGLAAPEARAIAGSLSLVPVG
                     LPGKFVQEMPDARPDLQLITPMDVQKALLRRERASHKGSYGAALLLGGSAKYAGAIVL
                     ATEGAIRSGTGLTRVVTVESSVHPILARVPEAIVEGSLSPDVVLPPASAILVGPGLTR
                     DPEARRLVARLLLETSAPLVLDADAIAVLEGKPEVVKKCAQPVILTPHPGELAMLLGT
                     DAATIQQDRLAAVRTAVERTGATVILKGAGTLVAQPDGPVWINLNGNPGMATGGTGDV
                     LGGLLTGLLAQKIPPLEAACLAVWLHGVAGDIAAVRYTQTAMKASDIAAALPEAFRQI
                     TIR"
     gene            complement(7861..9219)
                     /gene="rlmD"
                     /locus_tag="PKI64_06795"
     CDS             complement(7861..9219)
                     /gene="rlmD"
                     /locus_tag="PKI64_06795"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOE00752.1"
                     /translation="MKKQPQEWTLRTEKMVYGGPALARADRFVVFVDDALPNETVRAR
                     IYKKKKQFAFGRAVEILEAAPERVPSDCPWTGDCGGCTYRHCTYPAQLRFKQEVLAEA
                     LHGVPGAAACLHAVVPAAATQDYRNKMVFTFGTTPTGELRLGLHRRGSFMHIIPADTC
                     WLQSAASREIVRRVQALAKQLNAPAFHEIKKTPGLRTLTVREARGTGQRMVELVTTTI
                     YPGLAEALLAQLDDLADTIFLSTDTNIYGSARPEQRTILKGTGIIEETLNGLRFRIGP
                     DTFFQSNSAQAAALFRRLTELATQHGRPRVALDLFSGTGPIALHLAAHAERVIAVENW
                     APSVEVLRENLALNNVQNVDAVAADINQSHPPGIPSRVDLVVVDPPRPGLSPRAIEWV
                     TSRQPESVIYVSCNPATLARDLKLFLQAPYQIETIEPFDLFPHTYHIETLVLLRRTST
                     SE"
     gene            complement(9222..9680)
                     /gene="smpB"
                     /locus_tag="PKI64_06800"
     CDS             complement(9222..9680)
                     /gene="smpB"
                     /locus_tag="PKI64_06800"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOE00753.1"
                     /translation="MTTRAQATGALATNRKARHEYQILDSWEVGLALTGGEVKSCRAG
                     QASLAEAYAAFEKGELFIHGMRIQPYAAAKSGFNTHEPDRPKKVLMHRRELDRLAGTL
                     QLKGHTLVPLKLYLARGKIKMELGLGKGKLLYDKRESLRRKTEAREAART"
     gene            complement(9698..9943)
                     /locus_tag="PKI64_06805"
     CDS             complement(9698..9943)
                     /locus_tag="PKI64_06805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOE00754.1"
                     /translation="MNATYLEKAKEKMPNIPLLVNVVSKRVRQLNAGQRPLIKPDTPD
                     MDHLDLALKEIAEGKLSAELGEVTSPKADLGRSLLAF"
     gene            10164..10240
                     /locus_tag="PKI64_06810"
     tRNA            10164..10240
                     /locus_tag="PKI64_06810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10198..10200,aa:Arg,seq:acg)
     gene            complement(10337..11470)
                     /locus_tag="PKI64_06815"
     CDS             complement(10337..11470)
                     /locus_tag="PKI64_06815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOE00755.1"
                     /translation="MKKFACLLLAVVTVGLLPIAAFALGVGDPAPELKAAQWVKHGPV
                     DALDPAQTYVVEFWATWCGPCRSTIPHLTQMARQFTNVTFIGMNVWERGADPAPRVTE
                     FVNSMGSKMDYAVAMDTTNWFMAKNWMEAAGQNGIPAAFVVHQGRIAWIGHPMGDLQE
                     TLQAITAGTFNLETVRQRADAEQRVQNFVVKLMEGASDEEVAAEGAAIEALQADVGNL
                     GPDGTPFVTADMIQLVHKQKVEMETRQKAMKTIEQYLAAIGPKGSAEEAAKMGAELEA
                     LDLRNPGILNEIAWHILTGEDVQHRDLPLATRLAKKAVELTAERRGEILDTYARALFD
                     AGQVAEALAWQQKAARVAPNDREIADTLQRYQAAAQSAAPAQE"
     gene            complement(11595..12980)
                     /gene="thrC"
                     /locus_tag="PKI64_06820"
     CDS             complement(11595..12980)
                     /gene="thrC"
                     /locus_tag="PKI64_06820"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOE00756.1"
                     /translation="MRYVSTRGNQPPLTFKDAVMTGLARDGGLLIPDRIPDISAQLRG
                     WADLDYPDLAFEIIRLFVDDIPPARLRELINAAYATFDRPEVTPLRRVGPVHILELFH
                     GPTLAFKDIALQFLGRLFAEILEERDGRLNILAATSGDTGSAAICGVRGQRRIHIFVM
                     HPHGRVSALQERQMTSVLDDNVFNLAVKGSFDDCQLIMKTLFNDLAFRDAHALGAVNS
                     VNWARVLSQIVYYFYAAFRVMAQTRSAQVQFAVPTGNFGDILAGWYAKRMGLPIRRLI
                     LATNENDILARFFNTGVYATGRVVPTVNPSMDIQVASNFERWLYYKVGENPTEVARLM
                     TTFALQGSMSIPCDEHGHVDLEIVAGTSDTRRTLATIKRYHEKHSYLLDPHTAAGVFV
                     AEQYNELDEPLICLATAHPAKFPEAIEDATGEDLAHHPTLEALKDAPTRCEVIENHAG
                     AVKDFLAARAI"
     gene            complement(13044..13994)
                     /locus_tag="PKI64_06825"
     CDS             complement(13044..13994)
                     /locus_tag="PKI64_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00757.1"
                     /translation="MISAVLLFAAGIGLLWSGTSTVLRRVPKLAAWLRVSPLVITVLL
                     LAIMTSLPEFCVSLFAALRGQHSAAMGNIVGSNFVTLTFVAGLCALWRPIVVGPTIRE
                     RESSWMILSAGLLLVLALDGQLSRGDGLVLLLAYLPYFHRTLMEAKQQRQAEAGSAAV
                     VPHPGRDILLLLLGLGMIVLGANWIVVNGTTLAKALGMSDLLIGVTFYAFGTSLPELA
                     IALGAVIHRHADVALGEIYASNIFTGLAVAGALALILPLPVEPIIVQRDLPLLVVAGV
                     LLKMFVTSGGRFVRTEALAMIAIFAFFLSAQFLGFGISLP"
     gene            complement(14143..14228)
                     /locus_tag="PKI64_06830"
     tRNA            complement(14143..14228)
                     /locus_tag="PKI64_06830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14193..14195),aa:Leu,seq:taa)
BASE COUNT         2708 a         4157 c         4703 g         2674 t
ORIGIN      
        1 ttcggggtgg aacaggacag ccacaaacca agggtgggcg ggcaattcga tgacttgcac
       61 atcgtgcccg gctggatccg tgccgctgca aaccatgccg gcggcttcga gttgcgggcg
      121 ataggcgggg ttcagttcca gcgcgtgccg gtggcgttca gatatccggg tggcgccata
      181 gattttggcc aggagggaat cgggtttcaa tgcgcagggc ttggcgccga gttgttcgcc
      241 gtgcggtgcg gcgtgcagca ccgggtgagg ggtatcggga gcggtgtccg tgtcgttggc
      301 ttccgtcaga ccgcacacgt ggcgggcgta ttcgatcaca gcgcaatgga agccgcgccc
      361 aatgcccaaa aagggcaagc cggtttcgcg cgcgtggcgc accgcggcca accgcccgac
      421 caaaccgtcg gcatcgccgc catcggggat gagcagcgca ttgatttccg gcaagcgggc
      481 aggggctttg ccggcagtga aatcgccggc gggcagcggg ataatttcca ccgattgctg
      541 atgcgccccg ccgccgtggc gcagggcttc gtggagagat ttatgggtgt cgggcagttc
      601 tgcgcgctgc ccaaccacgc caatccggac ggtgccgcgc ggctgggtga tggcggccac
      661 catggcccgc cagtccgcca gttgggcggg cgggcgcggc aggcggaaat gcaccaggat
      721 gatttcatcc aagccttgtt cggcaagcac cagcggcact tcgtggatgg tatggcgcac
      781 atccagctct tcaatgacgg cgtgcaacgg cacattgcaa aagagcgaga gcttgcggcg
      841 aatgtcttgc gtgagcggtt gctccgagcg gcagaccagc acatcgggca cgatgccgat
      901 ttcgcgcagt ttggccacgc tttgttggct gggtttggtt ttgagttcgc cggccgcgcg
      961 aatcagcggc agataggtca tgtggatata caagacccgc tcgcggcctt cctcgaggcc
     1021 gatttgacga atggcttcca gaaacggcag gccttcgata tcgcccaccg tgccgccaat
     1081 ttcggtgagc acgacatcca catcgggcgc ggcgagggcg cgaatgctgg ctttgatttc
     1141 gtcggtgatg tggggaatca tttggacggt gtggccgcgg tattcgccgc gccgttcttt
     1201 ttgcagcacg tcccaataaa tgcgtcccga ggaaaccgac gattggcgcg tggtgggctg
     1261 gccggtgaag cgctcgtagt ggccgaggtc cagatcggcc agggcgccgt cgtcggtgac
     1321 gtagacttcg ccgtgctgat cggggctcat ggagccgggg tccacgttga ggtaggggtc
     1381 catcttcagc atgcggatgc gcaaaccgcg gctaatcaac agccgcccga tggatgccgc
     1441 ggtaatacct ttgcccagcg aggaaaccac gccgccggtg atgaaaataa aatgcgccat
     1501 gaggtctcct gcggggttca atccagagcg atgatggcgg caagcacctg cggggtggcg
     1561 cgcaagcggt cgtagaggcg cagcatgcaa tcgctatagg catacatcca ctgctgacgg
     1621 taagcccgtt cttgaatgtc cgaatatagc gcggcggtga aacaaatccg gcgggccgcc
     1681 gcaacaggcg gtccatccag atgcagttgc cggcggatga acaacgtgtt gggattcggc
     1741 gaggccagca gcgacggcat cagcgcggcg aaccagcgcc agtgccgctg gcggaattcc
     1801 aagagcgata ccggcggcgt ggtgtagcgg tgcaggcgct gcatgttctt cacgatttcg
     1861 cggcggtcct tgatttccgg cgtgcgcaga aacatcagca aaaagttgcg gtattgccgt
     1921 tcccattcca gatgcggctc atccgggtgc ggggggatga tgccggcttg cttcatgcgc
     1981 tgaatcaagt gatcaatgaa gacgcacgcc atgcgcgtga tcagcagcgc cttggtgagt
     2041 ttgacctgat ccgccgacca gccccagtgg cggcctagac gatccacgcg gtccgaggca
     2101 cgcactatgc gccgccagta ggcgaggttg aagccgggat gatgccccag cacctgttcg
     2161 gcgcggcgca ggtagcgttg gcgggcgatt tgccgattat agttggtgat cgggtctggg
     2221 agggccagtt ccatgacggc ggccgcgcgt caggcgccca gttgctcctg atagaggcgc
     2281 agggccgtca ggtaacgggc gtagctcccc ttactgaact tgtgcgggta ggcctcgaag
     2341 tgttgatgga tatccgacgg cgcggccgtg aggccggcga tttcgcgcaa gcgcgcgcgc
     2401 ttggtgccat caggcagcgg gggccacgca aagttttcaa agtcgagcgc gccttgcagc
     2461 accgggggcg ggattttctt ggcgtttttg atcaggtgca ccaccaggtt ttggagcgcg
     2521 cggccaaagt tgtcttcaat ttcgtttttc atggtgtttt acactcgcac gggcagagca
     2581 tagaacgggc cgccgccaaa cacaacggaa aaccaacggg tttatacgtt ctgataatcc
     2641 gggcccacgc cgtgctccgg cgtttcccag cggatgttgt cataggggca cttgagcgtg
     2701 cagcagcggc attgcacaca attttctgcg cggatgtgga tggcggtgcg tgtggcgtcg
     2761 gcttcataga ccccagcagg gcaaaagcgg gtgcagggtg cggcaaagcg ctggaagcat
     2821 tcgcggcatt tgtcggcatc agggatggcg atgtggtcgg tatcgggccg gtagcgcagg
     2881 cgcgccagaa agaggtcgga gtccagtccg agatccagcg ggccgttgtc gggctggggc
     2941 agggggggct ggcggtggcc gatttcgtgc aggcattggc gttcatcgcg ctggcgaatg
     3001 cggccccacg gcaggcgccc gccggaaatc caggccagtc cggccgcagc catgccgatg
     3061 ggcaagctgg cgcggaaagc ggcgcggtag ttcgcggtgc gccgtaggcc atccaacgac
     3121 ggcatgtctt cctgccgtag cgcatcgccg cggatgatgg catccgccgc ggccatgccg
     3181 gattccaccg ccaaatgaat gcccttcaat tccatggtgt ccaccaagcc ggcggaatcg
     3241 ccaatgatga aggccccggg cgcggccatg cccagccgcg aatgccagcc gccttcggga
     3301 accagccgcg cgccatattc caccgttttg ccgccggcaa tatgtcgctg cacaaagggg
     3361 tgccgtttcc agcgacggaa caaatcgtgc ggataggtgg cggggtcgtg gtaatccaag
     3421 gcaatggcca agccgagcgc cacgtgggtt tcatccatgt ggtagatgaa accgccgcca
     3481 taaatgttga tgccgagagg atagccgaag gtgtgcgcca cggtgccgag cagcgccgga
     3541 ttggcaggca cttccacgat ttccttgatg cccagcgcat aggtttgcag attcggcccg
     3601 gccaattgcg gatgccgcgc cagcacggcc ttggtcagca acccagcggg cccttccgcc
     3661 aacaccgtgg tatcggccaa aatggggtca tcgagggtgc ggacaccgct cacgcggtcg
     3721 ccttcccaga gcagcgcatc ggcggtttgg ctggtcagga tttccacgcc cagcttggtg
     3781 gcgatgtcgc ccaaggcgct gcccagttgg gccagcgaga ccagcggcag gccgcgcatc
     3841 cgcatttttg gtggcacaaa tggcacgcgg atggcgccct tgggcagcag cgcgtgaaag
     3901 gtgtcgcgcg tgacgagcgg gccgatgggg agggcggcgt attcgtcggg catgagcagg
     3961 tgctgcaagg cttgcggtcg catgaccgcg ccggacagca aatggccgcc aagctgggcg
     4021 gatttttcca gcaccatgat ttggcgctgg cccttgccag ctcgcgccag ccggatggca
     4081 caggccagcc cggccgggcc cgcgcccacc accagcacat ccactttcaa ttgcgcttcc
     4141 gcagcttgca tctgggaaaa cctagcaaag cgcgccggga ttacaagaaa ggacaaccgt
     4201 gccgtcgcgc cgctttgcgc cgggggattg ttttgacact gacttttgcg gaatgataca
     4261 agcaagggcc atgtgggcgg cccgtccggc gcgcccgccc ggagtattgc atatggccaa
     4321 agttgttctc gaaaacgtgt ataaaatcta ccccggcgat gtgaccgcgg tgcacgatgc
     4381 caacctggaa atccaggatc aggaatttgt ggtgctggtg ggaccctcgg gctgcggcaa
     4441 atccaccacg ctgcgcatgg tggcgggcct cgaagacatc agcaagggct ctatctatat
     4501 tgacggcaag aaagtcaatg acattccgcc caaagaccgc gacattgcga tggtgttcca
     4561 gaattacgcg ctctatcccc acatgagcgt gtataacaac atggcctttg gcctgaagct
     4621 gcggaaatat cccaaggccg aaatccacaa gcgtgtgatg gaagcggcgg aaatcctcgg
     4681 catcacggat tatctggagc gcaagcccaa ggcgctttca ggcggccagc ggcaacgggt
     4741 cgcggtcggc cgcgccatcg tgcgcaaacc caaggcgttt ttgttcgatg aaccgctttc
     4801 caacttggac gccaaaatgc gcgtccagat gcgcgcggag atcagcaaac tccacaaccg
     4861 gctcaagagc acgatgattt atgtgaccca cgaccagatc gaagccatga ccatgggcga
     4921 tcgcatcgtg gtaatgaagg atggctggat tcaacaagtg gatacgccgc tgaacatcta
     4981 taacaagccg gcgaaccaat ttgtggccgg gtttatcggt agcccgccga tgaatttctt
     5041 caagggcaag attgtgcgcg aggggaatgc cgtggctttt gccgaagggc gcttcacggt
     5101 ggccttggct ggcgcggtgg ccgacaaaat ggcggcccat gccgggcagg ccgtcaccat
     5161 gggcctgcgg ccggagcaca ttgacgagcg ttccgcccgt ccggatgcca acccggcgag
     5221 tgtagtgcag gcacaggtgg aagtggtgga aatgatgggc tccgaggtgt atctctacgc
     5281 caacaccggg cagagcaact tcaccgcccg cgtggactcc tcctgccgca aacaaatcgg
     5341 cgacaccgtg gaagtggttt tcgacatgtc caaggcgcat ttctttgccg aagacgacgg
     5401 ccgcgcgctg ctgtaggagc gaacgatgag tcttgcctgg gtggaactga ttcaaaccat
     5461 cgctgtggct gtggtggcct atgtgctgct gacggcgctg gcgcttttct tcaaggctga
     5521 agccgccggc cggcaagccc cgccggagtc gcccgcgcca tgaaattggg cgtgaacatt
     5581 gaccacgtgg ccaccctgcg ccagcagcgc ggcacggtgt atcccgcgcc cctggaagcg
     5641 gcggcgttgt gtgaaaaggg cggtgcggat ggcattacca ttcacctgcg cgaggatcgg
     5701 cggcatattc aggacgccga cttgttcgcg ctgcgcaagg cgctgcgcgt gccgttgaat
     5761 ctggaaatgg ccaacagccc gcaaattgtg cagatcgcgt tgcgcgcgcg gcccgccgag
     5821 gtgtgcctgg tgcccgaacg ccgccaagaa ctgacgaccg aaggcgggct ggatgctgcg
     5881 gggcagttcc gccggctcaa gcccaccatc gccaaactac aagctgccgg catcgtggtg
     5941 agcttgttca ttgagccgga cccgcgccaa ctggaagcgg cggttgccct gggtgcgccc
     6001 gtggtggagc tgcacaccgg caagtattgc gatgaaaccg gcgccgcgcg ccgcgcggaa
     6061 ttgaagcgcc ttgtggcggc cgccaagttg ggccaccgcc taggcctgca agtcaatgcc
     6121 ggccacggct tgaactatga caatctgccc gcttttctgc gggcagtgcc gcacttgcac
     6181 acattgaaca tcgggcatag catcgtggcg cgcgccgtaa tggtcggcat gacgcgcgcg
     6241 gtgcgcgaaa tgaaagcctt gttgaagcca tgaaatttgt caccgccaaa acgctgcgcg
     6301 ccgccgaagc caaggccatt gcgctggacc ggtcgttggc gctatccatg atggactggg
     6361 cggggaaagg gttggcccgt gtggtgcagg atgtcgccgc gcagatgggc cggcgcaacg
     6421 ccgcagtgcg tttgctggcg ggcccgggta acaacggcgg cgatgcgttt gtcgcggcga
     6481 tgagcctgca cgaggctggc ctgttgcccg aagtgtggct ggtgggcccg cgcgaaaaat
     6541 tgcgcggttc ggccaagtat tattttgatg aactgcaaaa gagcggcgtg ccgtggcgcg
     6601 aaatggtggg cgagaaagca tggcagcagg ccgcgggcct caccttgacg ccgcccattc
     6661 atgtggatgc gctgctgggc accggggcgc ggggcgagct gctgggcgat atccgccgcg
     6721 cggtggaata cctgcgcgaa aagcagacgc gctcaatcat tgtggccgcc gacgtgccca
     6781 ccggcatgaa ccccgacaca ggggaagcgg gcccctcttg cgtcacggca gatttcaccg
     6841 tgtccatgtg catgcccaaa atcggtttgg cggcgccgga agcacgcgcg attgcgggat
     6901 cgctttcgct ggtgcctgtt gggttgccgg gcaagtttgt gcaggaaatg ccggatgccc
     6961 gacccgattt gcagttgatt acgccgatgg atgtgcaaaa ggccctgctg cggcgcgaac
     7021 gcgcatcgca caagggcagt tatggcgcgg cgttgctgtt gggcggctcc gcgaaatatg
     7081 ccggagccat cgtactggcg acagaaggcg ccatccggtc cggcacgggc ctcacccgcg
     7141 tggtcaccgt ggaaagctcg gtgcacccca ttttggcgcg agtgccggaa gcaattgtgg
     7201 agggttcgct cagcccggat gtcgtcctac cgccggcctc ggcaattttg gtcgggcccg
     7261 gactcacgcg cgacccggaa gcccgccggc tggttgcgcg cctcttgttg gagacctccg
     7321 cgccgctggt gctggatgcc gatgcaattg ctgtgctcga gggtaagcca gaggtggtga
     7381 aaaagtgtgc gcaaccggtc atcttgactc cacaccccgg cgagttggcg atgctcctgg
     7441 gcaccgatgc ggccacgatt caacaagacc ggctggccgc ggtgcggacg gcggtcgagc
     7501 gcacgggcgc caccgtcatt ctcaaaggcg cgggcacgtt ggtggcccaa ccggatggcc
     7561 cggtgtggat caatctgaac ggcaatcccg gcatggccac ggggggcacc ggcgatgtgc
     7621 tgggcggttt gctgaccggt ctgctcgcgc aaaaaatccc gccgcttgaa gccgcctgcc
     7681 tcgcagtttg gctacatggg gtggctggcg acattgccgc cgtgcgctac acgcaaacgg
     7741 ccatgaaagc cagtgatatc gccgcggcct tgccggaagc cttccggcaa atcaccatcc
     7801 gctaataaca cgcgcaaatt gtgaagccgg cgaggggagg gtgcgggaag cgtctgggcc
     7861 ttattcagag gtgcttgtcc gccgcaacag aaccagcgtt tcgatgtggt aggtgtgcgg
     7921 gaacagatcg aaaggctcaa tggtttcaat ctggtagggc gcctgtaaaa agagtttcaa
     7981 atcccgcgcc agcgtggcgg ggttgcagga gacgtagatc acggattccg gttgccggga
     8041 agtcacccac tcaatagccc gcggcgaaag ccccgggcgc ggaggatcca ccacgaccaa
     8101 atccacgcgg gacggaatgc cggggggatg ggattggttg atatccgccg cgaccgcgtc
     8161 cacgttctgg acgttgttca acgccagatt ttcgcgcagg acctccacgg atggcgccca
     8221 gttttccacg gcgataaccc gctccgcatg ggcggctaaa tggagcgcga tcggtcccgt
     8281 gccgctaaac aaatccaacg ccacgcgcgg ccggccgtgt tgcgtggcca gctccgtcag
     8341 ccgccggaat agcgcggcgg cttgggcgct attgctctgg aaaaaagtgt cgggcccaat
     8401 ccggaagcgt aggccgttca gggtttcctc aatgatgccc gtccctttga gaatggtacg
     8461 ttgttcgggg cgggcggagc cgtagatgtt ggtgtcggtg gaaaggaaaa ttgtgtcagc
     8521 cagatcatca agctgggcca gcagggcttc cgccaggccg ggatagatgg tggtggtgac
     8581 cagttccacc atgcgctggc cggtgccgcg cgcctcccgc acggtcaggg tgcgcaatcc
     8641 cggagttttt ttgatttcat ggaaagccgg tgcattcaat tgtttggcca gcgcctgcac
     8701 gcggcgcaca atttcgcgcg aagcggcgga ttgcagccag cacgtgtccg ccggaataat
     8761 gtgcatgaaa ctgccccgcc ggtgcagccc cagccggagc tcgccggtcg gcgtggtgcc
     8821 aaacgtgaag accatcttgt tgcgatagtc ctgagtggcg gcggcaggaa ccaccgcgtg
     8881 caggcaggcc gcggcgcccg gtacgccgtg cagcgcctcg gccagcactt cctgtttgaa
     8941 gcgcaattgc gcgggatatg tgcagtggcg atacgtgcac ccgccgcaat cgcctgtcca
     9001 tgggcagtcg gaaggcacac gctccggggc cgcttccagg atttccaccg cgcggccaaa
     9061 cgcaaattgt tttttctttt tataaatccg ggcgcgaacg gtttcatttg gcagggcatc
     9121 atccacaaac accacgaaac ggtcggcgcg ggccaatgcc ggcccgccat acaccatttt
     9181 ttcggtgcgg agcgtccatt cttgcggctg ctttttcacg atcaggtgcg ggcggcttcg
     9241 cgggcctcgg ttttgcggcg caggctttcg cgtttgtcat agagcagctt gcctttgccc
     9301 agccccagct ccattttgat tttaccgcgt gccagataga gcttcagcgg gaccagcgtg
     9361 tgccccttga gttgcaacgt gccggccaga cggtccaact cgcgacggtg catcagcact
     9421 tttttggggc ggtcgggctc gtgggtgttg aagccggact tggcagcggc ataaggctga
     9481 atgcgcatgc catggatgaa cagttcgccc ttttcaaagg cggcataggc ttctgccaag
     9541 ctggcctggc cggcgcggca gcttttgact tcgcccccgg tgagcgccaa gcccacttcc
     9601 cacgagtcca gaatctgata ttcgtggcgg gccttgcgat tggtggccag tgcgcccgtg
     9661 gcctgtgccc gtgtggtcat ggggcgccgc gtggcggcta gaacgcgagc agcgagcggc
     9721 cgaggtccgc cttgggcgaa gtcacttcgc ccaactccgc cgacagcttg ccttccgcga
     9781 tttccttcaa tgccaaatca agatgatcca tgtccggggt gtccggcttg atcagcggcc
     9841 gctggccggc attgagctgc cgaacgcgct tcgagaccac gttgaccaac aacgggatgt
     9901 tgggcatctt ttccttggct ttctccaaat atgtcgcgtt catgcttgtc ctccgcaaat
     9961 tacaaattga ttcgaacgcg gcactatagg gggccaaacc ggcagcgtca atggcgaaaa
    10021 ccgcattttc tgcgcgcgtt acttgcaaat gtggccggag ggacgggcct cgtcagcatg
    10081 gcgcagggcg tgccgcgcca caaaaagtcg caaatggggg attgtcaaac cggggggcgt
    10141 ggtgtacaac tgcggcttat tgcgtacctg tagctcaatt ggatagagca tctgactacg
    10201 gatcagaagg ttacaggttc aactcctgtc aggtacgcca tattgttgcc ggcgcacagc
    10261 tcgccggcct ttttgttggc aggcaaaccg ggcgcgagtt gcctcgcgcc gtagcgccgg
    10321 ccgaggtttt atcgcgtcac tcttgtgccg gagcagcgga ctgggccgcg gcctgatagc
    10381 gctgaagagt atccgctatt tcccgatcgt tgggcgccac ccgcgcagcc ttttgctgcc
    10441 acgcgagcgc ttcggcgact tggccggcat cgaacagagc gcgggcatac gtgtccaaaa
    10501 tttcgccgcg gcgctcggcg gtcagttcca ccgccttttt ggcgaggcgc gtggcaaggg
    10561 ggaggtcacg gtgttggacg tcttcgccgg tcagaatgtg ccaggcaatt tcgttgagaa
    10621 tgcccggatt gcggagatcc aaggcttcca actcggcacc cattttggcg gcttcttcgg
    10681 cagagccctt ggggccgatg gctgcaagat attgctcgat ggttttcatg gccttttgcc
    10741 gggtttccat ctccaccttt tgtttgtgca ccagttggat catgtctgcc gtgacgaatg
    10801 gggtgccgtc gggccccaag ttaccaacgt cggcttggag cgcctcaatg gcggcgcctt
    10861 ccgcggccac ttcttcatcg ctggcgcctt ccatgagctt gaccacgaag ttctggacgc
    10921 gctgttccgc atcggcccgc tggcggacgg tctcaagatt gaaggtgccg gccgtgatgg
    10981 cttgcagtgt ttcctgcaaa tcgcccatgg ggtggccgat ccacgcgatg cgtccttgat
    11041 gcaccacaaa ggccgccgga atgccgtttt gcccggcggc ttccatccag tttttcgcca
    11101 taaaccaatt ggtggtatcc atggcgacgg cgtagtccat tttgctgccc atggagttga
    11161 cgaactcggt cacccggggg gcgggatcag cgccgcgctc ccagacattc atgccgatga
    11221 aggtcacgtt ggtaaattgg cgggccattt gggtcaggtg cgggatggtg gagcggcagg
    11281 gcccgcacca cgtggcccaa aactccacca cataggtttg ggcaggatcg agcgcatcca
    11341 cggggccatg cttgacccac tgcgcggctt tcaattccgg ggccggatcg ccgactccca
    11401 aagcaaatgc cgcgatgggc aggaggccaa cagttacaac agcaagaagc aggcaggcga
    11461 atttcttcat ggcggcagtc ctttggttag gggtgggggg ggcagctcaa cagagccatt
    11521 caacgccaca atacccggcg aagcaagcgc ggaatggctg gccccgggtt ggttcagctc
    11581 accgcggtgg tggctcaaat ggcgcgggcg gccaggaaat ctttgaccgc accggcgtgg
    11641 ttttcaatga cctcgcaccg ggtcggtgca tccttgagcg cttccagtgt agggtggtgt
    11701 gccaagtctt cgccggtggc atcttcaatg gcctcgggga atttggctgg gtgcgcggtg
    11761 gcgaggcaaa tgagcggctc atccagctca ttatattgct cggccacgaa cacgccggcg
    11821 gccgtgtgcg ggtcgagcaa atacgaatgc ttttcgtggt agcgcttgat ggtggccagc
    11881 gtgcgccgcg tgtcggaggt gccggccacg atttccaagt ccacgtggcc gtgctcatcg
    11941 cacggaatgg acatgctgcc ttgcagcgcg aaggtggtca tcaggcgggc cacttcggtg
    12001 gggttttcgc caaccttgta atagagccag cgctcgaagt tgctggccac ttgaatatcc
    12061 atggacgggt tgaccgtggg aaccacgcgc ccggtggcat agaccccggt gttaaaaaag
    12121 cgcgcgagaa tatcgttctc gttggtggcc agaatcaggc ggcggatggg gaggcccatg
    12181 cgcttggcat accagccggc aaggatgtcg ccaaaattgc cggtgggtac cgcaaattgc
    12241 acctgcgccg aacgggtttg cgccatgacg cgaaaggccg cgtaaaagta atacacgatt
    12301 tggctcaaca cccgcgccca gttgacggag ttgaccgcac ccaaggcgtg ggcgtcgcgg
    12361 aaggccagat cgttgaacag ggtcttcatg atgagttggc aatcgtcaaa gctgcccttg
    12421 acggccagat tgaagacgtt gtcatccagc acagaggtca tttgccgttc ttgcagcgcg
    12481 ctgacccggc catgggggtg catgacaaaa atgtggatgc ggcgctgccc gcgtacgccg
    12541 caaattgcgg cggaaccggt atcgccgctg gtggcggcca gaatgttgag ccggccgtcg
    12601 cgctcttcca ggatctcggc gaaaagacgg cccaaaaatt gcagggcaat gtctttgaac
    12661 gccagcgtgg ggccgtgaaa gagttccagt atgtgtactg ggcctacccg gcgtagtggg
    12721 gtgacttccg gccggtcgaa tgtggcatag gccgcgttga tcagctcgcg caggcgcgct
    12781 ggtggaatgt catccacgaa aagccggatg atttcaaagg cgagatcggg atagtccaga
    12841 tcggcccagc cgcggagctg ggcgctgatg tcgggaatgc ggtcgggaat cagcagcccg
    12901 ccgtcgcgag cgaggccggt cataacggca tctttgaacg tcagcggcgg ctggttgccg
    12961 cgggtgctca catagcggat cggttccata gccgcgcatt tatagggtgt gccggggagg
    13021 gctgcaagtg ttttacagtt acctcaaggc agcgaaatcc cgaagcccaa aaattgcgcg
    13081 gacaaaaaga acgcaaaaat ggcaatcatg gcgagggctt cggtgcggac aaaccgcccg
    13141 ccgctggtga cgaacatttt gagcaatacc cccgccacaa cgagcaaggg caaatcacgt
    13201 tgcacaatga tcggctccac cggcagcggc agaatcaggg ccaacgcgcc cgcgacggcc
    13261 agccccgtga aaatattgga ggcataaatc tcgcccaacg ccacatcggc atggcgatga
    13321 atcaccgcgc cgagcgcaat ggccaattcc ggcaacgagg tgccaaatgc gtagaacgtc
    13381 acgccgatca gcagatcgct catgccaagg gctttggcca gcgtggtgcc gttgaccacg
    13441 atccagttgg cgcccagcac aatcatgccc aagcccagca gcagcaaaag aatgtcgcgc
    13501 cccggatgcg gtacaaccgc cgcgctgccg gcttcggctt gccgctgctg tttggcttcc
    13561 atcagcgtgc ggtggaaata tggcaaatag gccagcaaca ggaccagccc gtcgccgcgg
    13621 ctcaactgac catccagcgc caacaccagt agcaggcccg ccgagagtat catccaactg
    13681 gattcccgtt cgcggatggt cgggcccacc acaatgggcc gccacagcgc gcacaagccc
    13741 gccacaaacg tcagcgtgac gaagttggag cccacaatgt tgcccatggc cgcggagtgc
    13801 tgcccgcgca acgccgcaaa cagcgatacg caaaactccg gcagggaagt catgatggcc
    13861 agcaacagga cggtgatgac caagggcgat acgcgtagcc aagccgccaa tttgggcacg
    13921 cgccgcaaca cggtggaagt gccggaccac aggagtccaa tccccgcggc aaagagtaaa
    13981 acagccgaga tcatgtgcgc ctcggagcaa agggtggcca tgcaggacgc ggatgggcaa
    14041 gggtaggtcg cgttagcgcg ggtttcggtt gtccactcat cgtttgtttc ctcgtcggct
    14101 ggtgttcgaa ccccattcgc ttgccgtgcc ggccgacgag cgtggtgccc gaggcgggac
    14161 tcgaacccgc acggcctttc ggccaaggga ttttaagtcc cttgcgtctg ccatttcgcc
    14221 acccgggcat ggggtgccaa gc
//
LOCUS       DAOPNP010000061        14157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5039_length_14157_cov_8.584811, whole genome shotgun sequence.
ACCESSION   DAOPNP010000061 DAOPNP010000000
VERSION     DAOPNP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5039_length_14157_cov_8.584811"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKI64_06835"
     CDS             complement(<1..489)
                     /locus_tag="PKI64_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00758.1"
                     /translation="MNTLLGISIPTYKRPEQLLATVRSVAAAGGLEHEIPVYIADDSL
                     DDTNVATLATLAGEYPFLMVRRNERNLGIDGNILNSVNINECRYAWLLGEDDRLVPGA
                     VTKMLAVLESSTAPFLYANYSSVNSDFSRVLKPASLALAGDAVQSADTFWRQSAWSAG
                     FIG"
     gene            complement(486..1241)
                     /locus_tag="PKI64_06840"
     CDS             complement(486..1241)
                     /locus_tag="PKI64_06840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00759.1"
                     /translation="MSAPTISIVTACRNARAELPAAAAAVLAALGPHDEWVLQDGAST
                     DGSQEFLATLTDGRVRLESAPDQGIYDALNKAVRRAQGDFLLILGADDRLRLPLDELR
                     PLLRDDRTVYYGDVWRVESADRYAGPFDGAKLARTNICQQAILYPRAAFVGRAFDLRY
                     RQQADWAFNMACFADPQLRFEYIPLLIADYAQGGVSSVKMDEAFQRDYRRLLKAYFPL
                     RQRWRPTLVSWLSDLFRALPGVPPARQTPARNL"
     gene            complement(1238..2461)
                     /locus_tag="PKI64_06845"
     CDS             complement(1238..2461)
                     /locus_tag="PKI64_06845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00760.1"
                     /translation="MRVLLVNTYARSGGASVAAQRLCAALNRAGVEARLLVQNPEGSA
                     APDLLTTADRWAVRRPWLDALPTFFYRNRRTPHWGNAWLNNRATRQVISHFAPEVIHL
                     HWVNHGMLSVHDIARLRGPLVWTLHDSWVFTGGCHSPQDCLRYQQQCGYCPELRSQRE
                     QDLSWRGWQRKQTAWRGVSFTVITPSRWLAAAAASSSLMAGRRIVVIPNAVDTATLKP
                     GDRAAARHALGLPANAPVFLFGAHGAASDWNKGLDLWQAALPFVARQHPEAIALVAGL
                     SGGTLSGLPLPVRELGMLNPTQLAQAMLAADATVIPSRMENLPNIAAESLACGTPVAA
                     FAVGGIPEMIENGSTGFLAHPHEPESLAQALGRLLAQGAGMRAACITAAQGRYAPDLV
                     ARQHIELYQSLLESE"
     gene            complement(2458..3489)
                     /locus_tag="PKI64_06850"
     CDS             complement(2458..3489)
                     /locus_tag="PKI64_06850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00761.1"
                     /translation="MAQPRITVLMPVWNGEAYLSQALASILRQTHDDFELLVVDDGST
                     DRTAEILTACHDSRLRVLRNETRRKLSGALNRGIAEARGEFIARMDADDLMRPDRLAR
                     QVACLERNSDWICCGGAVRTLGCGSGTILRYPCEPAELPAFCLFYAPFAHPTVMFRRE
                     WFQRAGLAYDGAYYPAEDYELWTRILRQFPAGNLPQVLLEYRVHGQSMTGGEWSAMDE
                     QTIRVQEQLLRRLGMSPTAEQLRLHRAASLGRLPPSEESLEQAADWLQQLAASNRETR
                     QYPPERLASMLNYVWFRQTMAVVRAMGQTAWVLYRQSPLAFMGPEAAKHRWLVRLAAL
                     KAQFFPKPL"
     gene            complement(3489..4655)
                     /locus_tag="PKI64_06855"
     CDS             complement(3489..4655)
                     /locus_tag="PKI64_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE00762.1"
                     /translation="MSSQPTILVTGASGFLGGRLVEQLLAEGRWHVRAMAHRPGGAVR
                     LARLGVEIAWTDLLKPDEVRAAVEGVAAVVHCAYGTDPRAARATTVDGTRNLLQAARA
                     AQVKQVVHISTIGVHSYSPPPNVTEDSPCVKSRDDYCNAKIEAEQVVRNICPTATILR
                     MGNIYGPWSLPWTVRPLAHIGEGKASVVDSGQHASNMVFIDNAVEAIRLSLATERAAG
                     ETFFITDDPCSWAEMYGAYATWLGRELRSVDSNELRSYIWPAWYNRWGSLMREIWQGI
                     LVPSMRYTAFRAAVSPRLGAGLSRLWQKLPVAIRYRLVGDPMGRSVPAAAEFADSRPA
                     TLPPAGLLELYAGRTVFSNEKAERVLGYGPCVTRQEALERTRLWAEWARLIGTT"
     gene            complement(4652..5701)
                     /locus_tag="PKI64_06860"
     CDS             complement(4652..5701)
                     /locus_tag="PKI64_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE00763.1"
                     /translation="MIRLAVIGCGSVVQQYHLPALAAVPALQLAGLVDVNPQILAQVQ
                     ARWPTKQALADYRDLRDLDAVLIASPHSLHAAMAEHFLAQGIHVLVEKPMVLHSAEAV
                     QLITLARDRQAVFAVGVFRRFYPVSRLVKAMLQQQWLGGVVAVDAEEGGRYDWELQSR
                     YLLDRNLAGGGVLVDTGSHLIDRLLWWLEGANIQLVEYLDDATTGVEADCELRFRAAW
                     QGRDIPCRVELSRTRNLRNTIRLTLQEGWVELGANAPNGMRWSSAKLAQATEVAAQLW
                     LQSGTPDDAPVLPQAYFQQQLADFAQAIVSGSLPLNHAASNLPAVRLLEECYQQRRQL
                     PEPWNQPSVATGGQP"
     gene            complement(5698..6864)
                     /locus_tag="PKI64_06865"
     CDS             complement(5698..6864)
                     /locus_tag="PKI64_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00764.1"
                     /translation="MSSAPRNKNWLRRLLSPLGRQLWQWRCARQNRAVRKSAKQIRRG
                     PTPRGGALRVAYVHPRFPLAPQMAHIGGGGAVKYLWLAERFPHSFPECDAVYAVSSAH
                     HPQTAAVFAAARQRGIPIIWNQDGAFFPHSYGAAEAQAGNRAMGELFHMADYVLYQSE
                     FARSSSHLFLGEREEPGEVLYNAIDTNFYRPLPERRDPGHVLLAAGSHDDAYRLPLVL
                     DVFQIVRRQIPDARLLLAGRMAPAVLTAAQRRIEQEQWQSCVEWLGPYTPNATPFLFN
                     RAHLFLHVKYSDVCPSVVLEAMACGLPVAFSATGGTPELVGEAGIGVPSEQDWECPRP
                     PAAAALANAVVRIMGAREEYSRLARQRAVSLFDIQPWLARHEAVMREWMERRRS"
     gene            complement(6861..7967)
                     /locus_tag="PKI64_06870"
     CDS             complement(6861..7967)
                     /locus_tag="PKI64_06870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00765.1"
                     /translation="MATVLQLIQYVGRRRMAKLVAPVARRWQPYSRLMIDRDYAGWTL
                     DIEAKAVAAVGAALRIPVVAGAWQDYCRQQALYCVDQFVLLNPPDWQQHRVGIAYYHG
                     LPGSGPPEFDQVYQNLCRQHERLTRVQVSHAAMRDVVLDSGIAPEKVCVIPIGFHREW
                     FRPPIAGQRQRIRRRLGFPEDALVVGSFQKDGVGWGEGLEPKMIKGPDVFVSAMRQLH
                     ARVPTLHVLLTGPARGYVQRELTAAGIPYRHVHLRTYRQTADMYHALDAYVVASRQEG
                     GPKAVLESMASGVPLVSTRVGQATDLVQHGHNGCLVAVEDSEGLAHWTEQVLTQPAFR
                     ATVLAGGAQTAAANCYEAQHELWRDFFTPMVTMP"
     gene            complement(7970..8980)
                     /locus_tag="PKI64_06875"
     CDS             complement(7970..8980)
                     /locus_tag="PKI64_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00766.1"
                     /translation="MRSFPGKGILQRMGQRAGYLAARLAAQWRQFDISIFHQFAPPPD
                     GGGHQFLRALVRCLQERGWRVENNTISRTTRAVLFNSFNFDAERLRWLRRHGIPMIHR
                     LDGPLAAYRGFDDGTDRVTWSLNRRFASATIFQSAYSLAQHQAMGFAPAEARVIMNAA
                     DPLIFHARKREPFSLLRKIRLVSTSWSDNRNKGADVYEWLDNHLDWTRYEYTFIGRLP
                     IQPRNIRVEPPATSERVADLLRASDIYITASRNDPCSNSLIEALSCGLPAICLNSGGH
                     PELLRGGGETFSAAEEIPALLERVARDYADYQARIPTFDIEAVTTQYLAAMGLPAVPE
                     NR"
     gene            complement(8977..10095)
                     /gene="wecB"
                     /locus_tag="PKI64_06880"
     CDS             complement(8977..10095)
                     /gene="wecB"
                     /locus_tag="PKI64_06880"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOE00767.1"
                     /translation="MKTVLTILGTRPEAIKLAPVLHELARRRDVFRSKVCVTGQHREL
                     LEPMLEFFGIEPDYDLRVMRRNQPLAALTARLLSALDPVLAQLKPDWIVIQGDTTSVL
                     AGALGAYYRGCRVAHVEAGLRTDNKYSPFPEELNRRVASVLADAHFAPTARAVRNLRR
                     EGIPGATICLSGNTVVDALHWAVQRVKRHPPAWPDDVPGLSKERRLILVTGHRRENFG
                     AGLEQICRALRDIADELPDAELVYPVHLNPNVRGPVRRLLGGHPRIHLARPVAYPVMV
                     ALLRRAWLVMTDSGGIQEEAPTFGVPCLVMRDTTERPEGIRQGVAKLVGANRRHIVAA
                     VRRLAQDSRAYARMATGGNPYGDGQAARRIVDWLETGA"
     gene            complement(10105..11187)
                     /locus_tag="PKI64_06885"
     CDS             complement(10105..11187)
                     /locus_tag="PKI64_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00768.1"
                     /translation="MSTIQIHEPELRRRRGFNGFYGHFLSALVEHAGARDTTEPRPLP
                     RVIRPREHESCWVRWGTHWVFFDMSDHVQLFDLEALKLCSVYFKANLHRGIAGRILDE
                     AGLSEQWPKLAPFLFFSEGLGDFIRDANRRRFWRMDRPRYDVCFVMGVYANPVRDGGR
                     SPFEHPDEPMTPAAYHFWIRWHIQQALREAGIPGYYRLTSRANRALEDGQNVYGNLSR
                     RAFSRRITDGRITAVCTFPHALFPWKASESFVLGRPLLIEQTPLTETPTPFMPVAGEH
                     YLELLPDTGGFDPKAPLDDPASYRVLARIASAHIRERAEWLRDVLADRERLSAMGEAC
                     QVFAARAYHKKHVADYICAEVEKRIR"
     gene            complement(11361..11900)
                     /locus_tag="PKI64_06890"
     CDS             complement(11361..11900)
                     /locus_tag="PKI64_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00769.1"
                     /translation="MSSRFPLEWKLALGTVALLLIWLPWATPWVIRLVAREGDFVIRE
                     AAAPVKLPGTTYAIDRVEVSPDTRLEMVRVVGWALPPEAWQQSDTPLTECDVAFKGER
                     AWYRVPTVPVDRRDVRDILDVPGHQVCAGFQTRFSPVVLPPGIYRMALVIREGTNDLA
                     MAWTDKVFVQSSSGFQERE"
     gene            complement(11897..13384)
                     /locus_tag="PKI64_06895"
     CDS             complement(11897..13384)
                     /locus_tag="PKI64_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00770.1"
                     /translation="MVRLLKEYVYVLVIALYPVLLVYFRNVHEAGAEQLKLPILFFLA
                     VALLAWLFFSAWAGRFAVGALIAQLTLLIAALWRPLETAVRAQWVMIRYWHLFPVALA
                     VGVIAALLLGRRARLGKTRFLAYRKWLAGLFAILIVYNAAGAVPVLWRRVTQFRRAPA
                     APASVVPVAARVDRPDVYLIILDEFAPFLQIQKHFNWRPVELAQYLRQRQFSVSGSSY
                     NPSFLTVEVLAGLVGMEWPAEITQYQVAADGRIKKYVNTPDLDQHTYFAESTLMRFFQ
                     AHGYTVYVANMLGDLFNVSSPFVADYTFQLPPNAGGVSLENTVFSSVLKNSVLEPLLT
                     IWPHDKHFYNRMVEGILGWLADPNIRRAPSFTFAHIACPHAPYLFEPDGAWRKEEGSV
                     KVDDFYLAQYQFIAKRIIAVLEQIIAANPDCIVLVQSDHSYRHELTMPVDDMASIFNA
                     VYYRGRAFNIEGLSGLDTLRRVLNETFGTDFPIHEGRRPLAGLVQ"
     gene            complement(13443..>14157)
                     /locus_tag="PKI64_06900"
     CDS             complement(13443..>14157)
                     /locus_tag="PKI64_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE00771.1"
                     /translation="AVRKISPAREARYRNMLSRVDQFVAYGYAEAEWLREWLGTPDKV
                     QFVPFGVDVEQWRPMPDAPKIADVVSVGADPQRDFPLLLAYARQHPHVRVQLVVGQDA
                     AAKLMDVPPNVTVQVQLPVKRVQEVMAGARVLVLPVKENTYSGATTTLLQGMALEKAV
                     AVSRVGAIRDGYGLQDDLNIRWLEPGSQASMNVVLNDLLARPEACQRLGATARRHVVE
                     ELSWSRYVETMRQCIMKWI"
BASE COUNT         3098 a         4598 c         3893 g         2568 t
ORIGIN      
        1 gccgatgaag ccggcagacc acgcggactg ccgccaaaat gtgtccgccg actgaacagc
       61 atcgcctgcc agcgccaacg aggctggttt taacacccgg ctaaaatcgg aattcacgga
      121 agaataattg gcatacaaga atggcgcggt gctggattcc agtaccgcca gcattttagt
      181 aacggcaccc ggcaccagcc ggtcatcctc gcccaacaac catgcgtaac ggcactcgtt
      241 gatgttcacc gaattcagga tattgccatc aattccgagg ttgcgttcat tgcggcggac
      301 catcagaaaa ggatattcgc cggccagcgt cgcgagggtc gcgacgttgg tgtcatccaa
      361 tgaatcatcg gcgatgtaca ctgggatttc atgttccaat ccgccggccg ccgccacgga
      421 gcgcaccgtg gctaacaatt gttctgggcg cttgtacgtg gggatgctga tacccaagag
      481 agtattcata aatttcttgc cggggtttgc ctggccgggg gcacacccgg caaggcccga
      541 aacagatcac tcaaccaact gaccagtgta ggccgccagc gctgacgcag gggaaaatat
      601 gctttcaaca aacggcgata atcgcgttga aatgcctcgt ccatcttcac cgaactgacg
      661 cccccctgcg cgtaatcggc aatcaacaat ggtatgtatt caaaacgaag ctgcggatcg
      721 gcaaaacacg ccatgttgaa ggcccagtct gcctgctgcc gatagcgcaa atcgaaagcc
      781 cgaccgacaa acgccgcgcg cggatacaag atggcttgtt ggcagatatt ggttcgcgcc
      841 aacttggcgc catcaaacgg cccggcgtaa cggtcggcag actccactcg ccaaacatcg
      901 ccgtaataca ccgtacggtc gtcgcgcaac aaaggacgta actcatccaa cggcaggcgc
      961 agacggtcat cggcgccgag aatcagcaga aagtcgccct gggcgcgcct gacagcttta
     1021 ttcagcgcat catagattcc ctgatctggc gcgctctcca aacgcacccg gccatcggtc
     1081 agagtagcca gaaattcctg cgaaccgtcg gtagatgcgc catcctgcag cacccattca
     1141 tcgtgaggtc ccaacgcggc caaaaccgca gcagccgctg cgggtaattc cgcacgcgcg
     1201 ttgcgacaag ccgtcacaat gctaatggtg ggagcgctca ttcggattcc aacaacgatt
     1261 gatacaactc gatgtgctgc cgggcaacca gatccggcgc ataccgcccc tgcgcggcgg
     1321 tgatacaggc agcacgcatc ccagccccct gtgccaacag tcggcccagg gcttgcgcga
     1381 ggctttcagg ttcatgcggg tgggctaaaa aaccggtact cccgttttca atcatttccg
     1441 gaataccacc cacggcaaaa gcagccaccg gcgtgccaca cgccagactt tccgcggcaa
     1501 tgttgggcag gttttccatc cgcgatggaa ttaccgtagc gtctgctgcc agcatggctt
     1561 gggccagttg cgtggggttg agcatgccta attcgcgcac tggcaatggc aagccggaca
     1621 gcgtgccgcc ggacagacca gccaccaatg cgattgcttc cggatgctgc cgcgccacaa
     1681 atggcaaggc ggcctgccac aaatccagcc ccttgttcca atccgacgcc gcgccatgcg
     1741 caccgaacag gaacactggg gcatttgccg gaagccccaa cgcgtggcgt gctgccgcgc
     1801 gatcgcccgg tttaagggtt gccgtatcca ccgcattcgg aatcacgacg atgcggcggc
     1861 cggccataag cgaggatgaa gcagccgcag ccgccaacca ccggctcggc gtaatcacgg
     1921 tgaatgacac gccccgccat gccgtctgtt tgcgttgcca tccccgccag ctcaaatcct
     1981 gttcccgctg tgaacgcaat tccgggcagt atccgcattg ctgctgatag cgcaaacagt
     2041 cttgagggct atggcacccg cccgtgaaca cccatgagtc gtgcaacgtc cacaccaacg
     2101 ggccgcgcag gcgggcgatg tcgtgcaccg acagcatgcc atggttgacc cagtgcagat
     2161 gaatcacctc tggcgcgaaa tgggaaatga cctggcgtgt cgcacgattg ttcagccatg
     2221 cgtttcccca gtgcggcgta cgccggttgc ggtaaaaaaa cgttggcagg gcatccagcc
     2281 aaggacggcg taccgcccac cgatccgccg tggtcaaaag gtctggcgcg gcactcccct
     2341 cggggttttg cactaaaagc cgcgcttcca ccccggccct atttagcgcg gcgcataggc
     2401 gttgcgcggc caccgaggca ccaccactcc gggcatacgt gttgaccaac agaactctca
     2461 taaaggtttg gggaaaaatt gcgctttaag cgcagccaac cgcaccagcc aacggtgctt
     2521 cgctgcctcc ggccccatga aggccaaggg gctttgccga tacagtaccc acgcggtttg
     2581 tcccatggcg cgaacaacgg ccatggtttg tcgaaaccag acataattca acatgctggc
     2641 caaacgctcc ggtggatatt gtctggtttc gcggttggat gccgccagtt gctgcaacca
     2701 gtctgccgcc tgctcaagcg actcttccga agggggtaac cggcccaacg atgccgcgcg
     2761 gtgcagccga agctgctcgg ctgttggcga catccccaac cgccgcaata actgctcttg
     2821 cacccggatg gtctgctcat ccatggctga ccattcaccg cccgtcatgc tttggccatg
     2881 aactctgtac tccaataaaa cctgcggcag attgccggcg ggaaattgcc ggagtatgcg
     2941 ggtccagagt tcataatctt ccgcggggta ataggcaccg tcataggcca gccccgcgcg
     3001 ttggaaccac tcccgccgga acatgacggt aggatgggca aacggcgcat agaacaaaca
     3061 gaatgccgga agttcggctg gctcgcacgg ataacgcagt atcgtgccag agccacagcc
     3121 caaggtgcgt accgcgccgc cacaacaaat ccaatccgaa tttcgctcca gacacgccac
     3181 ctgccgcgcc aggcgatccg gccgcatcag gtcatcggca tccatccgcg caatgaactc
     3241 cccccgggcc tcggcaattc cgcggttgag cgcgccggat aatttacgcc gggtttcgtt
     3301 gcgcagcacg cgcaaccgtg aatcatggca ggcagtcaaa atctccgcag tgcgatcggt
     3361 cgagccatcg tccaccacca gcaactcaaa atcatcatgc gtctggcgca aaatgctggc
     3421 caaagcctgg gacaaatagg cttccccgtt ccagacgggc atcagaaccg ttattcgcgg
     3481 ttgtgccatc aggttgtccc aatcaagcgc gcccattcgg cccatagccg agtgcgctcc
     3541 agagcttctt gccgcgtgac gcacggccca taacccaaca cgcgctcggc cttttcgtta
     3601 ctgaaaacgg ttcttcccgc atacaactcc agcagtcctg cgggaggtag tgtggccggc
     3661 ctggaatccg caaactccgc cgccgccggc acggaacggc ccatcggatc gcccaccaag
     3721 cggtagcgaa tggcaaccgg caacttctgc cacaagcgcg acaaacccgc gcctagccgc
     3781 ggcgaaactg ccgcgcgaaa agcggtatag cgcatggaag ggactaggat gccctgccag
     3841 atttcgcgca tcaatgaccc ccagcgattg taccaagccg gccagatata ggagcggagt
     3901 tcgttcgagt caactgaacg cagttcacgt cctagccaag tagcataggc accatacatt
     3961 tcggcccatg agcaggggtc atcggtaata aaaaatgttt cgccggccgc tcgttccgtg
     4021 gccaaagaca accgaatggc ttccaccgcg ttgtcaataa acaccatatt cgaggcgtgt
     4081 tggccgctgt ccaccacgct ggctttgcct tcgccgatat gcgccaacgg acgtacggtc
     4141 catgggagcg accacggtcc atagatattg cccatgcgca ggatggtggc ggttgggcag
     4201 atgttgcgca caacttgctc ggcttcaatc ttggcgttgc aatagtcatc gcgcgacttc
     4261 acacacggcg aatcttcagt gacgttgggt ggcgggctat acgaatgcac cccgatggtg
     4321 ctgatgtgaa ctacttgctt aacctgcgcc gcacgcgccg cttgcaaaag attccgggtg
     4381 ccgtccacag tcgtcgcgcg tgcggcacgc ggatccgtgc cataggcaca gtgcactacc
     4441 gccgcgacac cctcaacagc ggcacgaacc tcgtctggct tgagaagatc cgtccaagca
     4501 atttccacac ccaagcgggc caaccgtacc gctccgcctg gccgatgcgc cattgcccgt
     4561 acatgccacc gtccttcagc caacaactgt tcgaccaaac gaccaccaag aaaaccgctg
     4621 gcccccgtca ccaagatcgt gggttggctg ctcatggctg acctccggtg gcgaccgaag
     4681 gttggttcca aggctccgga agctgacgac gctgttgata acattcctcc agcaagcgta
     4741 ccgccggcag attgctggcc gcatggttga gtggcaacga tcccgacaca atggcctgcg
     4801 cgaaatcggc caactgctgt tggaaatagg cttgtggcaa aacgggagcg tcatccggcg
     4861 tgccggattg tagccaaagt tgcgccgcga cttctgtcgc ttgcgctaat ttcgctgagc
     4921 tccaacgcat tccgttgggt gcgttggcgc ccagctccac ccagccttct tgtagtgtga
     4981 gccgaatggt gttgcgcaaa tttctggtcc ggctgagttc cacccggcat gggatgtccc
     5041 gcccctgcca tgcagcgcgg aagcgcaact cgcagtcggc ctcaacgcca gtcgtggcat
     5101 catccagata ctcaaccagt tgaatgttcg cgccctccag ccaccacaac aaccggtcaa
     5161 tcaagtggct tcccgtatcc accagtactc ccccgccggc caagttgcgg tcgagcaaat
     5221 agcgcgattg caattcccag tcataccgcc cgccctcttc cgcatccacc gccaccactc
     5281 cgcccaacca ttgctgttgg agcatggcct tcaccagccg ggaaacgggg taaaagcggc
     5341 ggaagacgcc aacggcaaac acggcctgcc ggtcgcgcgc gagggtgatc agttgcacgg
     5401 cttcggctga atgcaacacc atgggttttt ccaccaacac gtggatgcct tgcgccaaaa
     5461 aatgctcggc catggccgcg tgcaaactat gcgggctggc aatcagcact gcatccaaat
     5521 cgcggagatc gcgatagtcc gcaagtgcct gcttggtcgg ccatcgggct tgcacttggg
     5581 ccaatatttg cggattcaca tccaccaaac ctgccaattg caatgcgggc accgctgcga
     5641 gtgccggcaa atgatattgc tgcactacgg aaccgcaacc aattaccgcc aaacgaatca
     5701 tgaccttctc cgttccatcc actcgcgcat caccgcctca tggcgggcca accagggttg
     5761 gatatcaaaa agactcaccg cgcgctgccg ggccaagcgg ctgtactctt cacgcgcgcc
     5821 catgatccgt acaacggcat tcgccaaggc cgctgccgcg ggcggacgtg ggcattccca
     5881 atcctgttcg gaagggacgc caatacccgc ttcccccacc agttcgggag tgccgcctgt
     5941 ggcggaaaac gccaccggca acccacaggc cattgcctcc aacacgaccg acgggcagac
     6001 gtcgctatac ttcacatgca aaaacagatg cgcgcggttg aataagaatg gcgtggcatt
     6061 gggagtatat gggccgagcc attcgacgca ggactgccat tgttcttgtt caatgcggcg
     6121 ttgagccgcc gtcaacaccg ccggagccat gcggcccgcc agcaacaaac gggcatccgg
     6181 aatttgccgg cgcacgattt gaaaaacatc caagacgagc ggcagccggt acgcatcatc
     6241 gtgtgagccc gccgccagca acacatggcc ggggtcgcga cgttccggga ggggacgata
     6301 aaaattggtg tcaatcgcat tatacagcac ttctcccggc tcttcccgct cgcccaaaaa
     6361 cagatgagag gaactgcgcg caaattcact ttgatacaac acataatcgg ccatgtggaa
     6421 caattcaccc atggcacggt tgcccgcttg cgcctcggcc gcgccatacg aatgtgggaa
     6481 aaatgcgccg tcctgattcc atattatggg aatcccgcgt tgccgcgcgg cagcaaacac
     6541 cgcggcagtc tgcgggtggt gcgcgctact gaccgcatag accgcatcac attcaggaaa
     6601 gctgtgggga aacctttcgg ccagccacaa gtatttgacc gcaccgccgc cgccaatgtg
     6661 cgccatttgg ggtgccaaag gaaaacgagg atgcacatag gccacgcgca gcgccccccc
     6721 tcgtggcgtc gggccccggc ggatttgttt ggctgatttt cgcaccgcgc ggttttggcg
     6781 tgcacaccgc cactgccaca actggcgtcc cagcgggctc aagaggcgac gcaaccaatt
     6841 cttgttgcgc ggggcagagc tcatggcatg gtcaccatcg gggtaaaaaa gtcgcgccac
     6901 agttcatgtt gcgcctcata gcagttcgca gccgcagttt gcgcgccgcc ggccagcacc
     6961 gtggcccgaa acgccggctg ggtcaacacc tgctctgtcc aatgcgccag tccctcgcta
     7021 tcttccactg ccacaaggca gccgttgtgc ccatgctgca ccaagtccgt cgcttgaccc
     7081 acccgggtgg agaccagcgg cacaccggaa gccatggatt ccaagactgc cttgggaccg
     7141 ccctcctggc gcgaggccac cacataggca tccaaggcgt ggtacatgtc cgccgtttgc
     7201 cggtaggtgc gcaagtggac atgccgataa ggaatgccag ccgctgtgag ttcacgctgc
     7261 acatacccgc gcgcgggccc agtcagcaaa acgtgcaggg ttggcacgcg ggcatgtaat
     7321 tgccgcatgg ccgacacaaa aacatccggt cccttgatca ttttgggttc gagcccttcg
     7381 ccccacccca cgccatcctt ttggaaggaa cctaccacca aggcatcctc gggaaaaccg
     7441 aggcgccggc ggatgcgctg gcgttgcccg gctatcggcg ggcggaacca ttcccgatga
     7501 aacccgatgg ggatcacgca tactttttcc ggcgcgatcc cgctgtccag caccacatcg
     7561 cgcatggccg catggcttac ttgcacacgg gtcagccgct catgttgacg gcacaagttc
     7621 tgatatacct gatcgaactc cggcggccct gaacccggca ggccgtggta ataagcaatg
     7681 ccgacgcgat gctgctgcca gtcgggagga ttcagcagca caaattgatc cacacaatac
     7741 agcgcctgct ggcggcaata gtcctgccat gctcccgcca caaccggaat gcgcaaggcc
     7801 gcgcccaccg ccgcgacggc tttggcctcg atgtccagcg tccaccccgc gtaatcgcgg
     7861 tcaatcatca aacgggaata gggctgccaa cggcgcgcca ccggcgccac gagcttcgcc
     7921 atccggcggc ggcccacgta ttggatcagt tggagtactg ttgccacagt caacggttct
     7981 ccggaactgc gggcaagccc atcgcagcca aatactgggt ggtcacggct tcaatatcaa
     8041 atgtgggaat gcgcgcttga taatccgcat aatcgcgcgc cacccgctcc agaagtgccg
     8101 ggatttcctc cgccgcggaa aaagtttctc cgccgcctct caacagctcc ggatggccgc
     8161 cactgttcag gcaaatcgcc ggcagcccgc acgacaacgc ctcaataagc gagttggagc
     8221 aaggatcgtt gcgactggcc gtgatataaa tatcgctggc ccgcagcaag tcggccactc
     8281 gttccgacgt cgccggcggc tccacacgga tgtttcgcgg ctggatcggc agccgcccaa
     8341 tgaacgtata ttcgtagcgc gtccaatcca agtggttgtc cagccactca tacacatctg
     8401 cgcccttgtt ccggttgtcg gaccagcttg tggaaaccaa gcggattttg cgcaatagcg
     8461 aaaaaggctc acgtttgcgc gcgtgaaaaa taagtggatc agccgcgttc atgatcaccc
     8521 gcgcttcagc cggcgcaaac cccatggcct gatgctgggc caagctgtac gccgattgaa
     8581 aaatagtggc cgaagcaaat cgccggttga gcgaccacgt cacgcggtcg gtgccatcgt
     8641 caaacccacg ataagccgcc agcggtccat ccagtcggtg gatcatggga ataccgtgtc
     8701 gtcgcaacca ccgcaagcgt tccgcgtcga aattgaacga attgaacagc accgcgcggg
     8761 tcgtgcggga aatggtgtta ttctccaccc gccagccgcg ttcctgcaag cagcggacca
     8821 gtgcacgtaa aaactgatga ccgccgccat ccggcggcgg cgcaaactgg tggaaaatgg
     8881 aaatatcgaa ttgccgccac tgcgcagcca agcgtgcggc caaatagccg gcgcgctgcc
     8941 ccattcgctg caagattccc ttgccgggga acgatctcat gcgccggttt ccagccaatc
     9001 cacaatgcgg cgcgccgctt ggccatcccc gtacggattg ccaccggtag ccatgcgggc
     9061 ataggcccgg gaatcctgtg ccagccgtcg cacggccgcc acgatatgcc gccgattggc
     9121 tcccaccaat ttggcaacgc cctgccggat gccttcgggc cgctcggtgg tgtcgcgcat
     9181 taccaagcag ggcacgccga aggtgggtgc ttcctcttga atgccgccgg aatcggtcat
     9241 aaccagccaa gcccgtctaa gcaaggccac catgactgga taagccaccg gtcgcgccag
     9301 atgaatgcgt ggatgcccgc ccagtaggcg gcgcacgggg ccgcgcacat tgggattgag
     9361 gtgcaccgga tataccagtt cggcatccgg caattcatcg gcaatatcac gcagtgcgcg
     9421 gcatatttgt tccagcccgg cgccaaagtt ttcgcgccga tggccggtca ctaaaatcag
     9481 ccgccgctct ttgctcagcc ccgggacatc gtccggccac gctggcggat gccttttgac
     9541 ccgctgcacg gcccaatgca aggcatccac caccgtattg ccggagaggc aaatcgtagc
     9601 gccgggaatg ccctcgcgcc gcaggttgcg caccgcccgc gccgttggcg caaagtgcgc
     9661 atccgccaac acgctggcta cccggcggtt cagttcttcg gggaaaggcg agtatttgtt
     9721 gtctgtccgc aaccccgctt ccacgtgcgc cacccggcag ccccgataat acgcccccag
     9781 cgcgccggcg agcaccgagg tggtatcgcc ctgaatcaca atccaatcgg gtttcaattg
     9841 ggccaacacc ggatccaacg cgctgagtag tcgggcggta agcgcagcca gcggctgatt
     9901 ccggcgcatg acgcgcaaat cgtaatccgg ctcaatgcca aaaaactcca gcatgggttc
     9961 aagcaattcg cgatgctgcc ccgtgacaca caccttggag cggaacacat cgcgccgccg
    10021 ggccagttca tgcagcaccg gcgccagctt gatggcctct ggccgggttc ccaaaatcgt
    10081 cagaacggtt ttcacagcat catcctaacg gattcgtttc tccacttccg cacaaatata
    10141 gtccgccacg tgttttttgt gataggcccg ggcagcgaat acctggcagg cttcccccat
    10201 cgccgacaaa cgttcgcgat ccgccaacac gtcccgcaac cattcggcgc gctcgcggat
    10261 gtgtgccgac gcaatgcgcg ccagcacccg atagctcgcc ggatcgtcca gcggtgcctt
    10321 cggatcaaat ccccccgtgt ccggcaaaag ctccaaataa tgttccccgg ctaccggcat
    10381 gaaaggtgtt ggcgtttccg tcagtggcgt ttgctcaatg agcaagggcc gccccagcac
    10441 aaaactctcc gacgccttcc atggaaacag ggcatgcggg aatgtgcaaa ccgccgtaat
    10501 ccgtccgtcc gttatccgtc gcgaaaaagc acggcgcgac agattgccgt acacattttg
    10561 tccatcttcc agcgcgcggt tcgcccggct ggtcaggcga tagtagccgg ggattcctgc
    10621 ctctcggagc gcctgctgga tgtgccagcg aatccagaag tgatacgccg ccggtgtcat
    10681 gggttcatcc ggatgctcaa atggcgagcg accgccatca cgcacgggat tggcataaac
    10741 ccccatgacg aagcacacat catagcgcgg acggtccatc cgccaaaacc gccgccgatt
    10801 tgcgtcccga atgaaatcgc ctaatccctc cgaaaaaaat aaaaacgggg ccagtttggg
    10861 ccactgttcc gacaggcccg cctcatctaa tatccgcccc gctatccccc ggtgcaggtt
    10921 ggccttgaaa tacaccgaac ataacttgag cgcctccaga tcaaacaact gcacatggtc
    10981 gctcatgtcg aaaaacaccc agtgcgttcc ccaccgcacc cagcaacttt cgtgctcgcg
    11041 tgggcgtatc acccgtggca agggccgggg ttcggtcgta tcgcgcgcac cggcatgttc
    11101 gaccagcgcg gacaaaaaat ggccgtaaaa cccgttaaaa ccgcggcggc gccgcaattc
    11161 gggttcatga atctggatgg tgctcatggt gcgggctgcc gcctccacgc cggctgaccc
    11221 ggccgacaat gccgctgcaa cggggggatt ttctgttggc gctgacgtgt catggattgc
    11281 gtgcccgatc cggccggtgc cgtggttgtt gcggattcaa ccataatcgc tgggccgtga
    11341 gcaagaaccg ctcggccatc ctattcgcgc tcctggaaac cggaactgct ttggacgaac
    11401 actttatcgg tccaggccat ggcaaggtcg ttcgtgccct cgcgaatcac caatgccatg
    11461 cgatagatgc ccggcggcaa gaccaccggc gaaaagcgtg tttgaaaacc ggcgcacacc
    11521 tgatggccag gaacatccag aatgtcgcgg acgtcgcgac gatcaaccgg cacagtgggc
    11581 acgcgatacc acgcccgctc ccctttgaag gcaacatcgc actctgtcaa aggcgtgtcc
    11641 gactgctgcc atgcctcggg cggcagcgcc cacccgacaa ccctgaccat ttccagccgc
    11701 gtatccgggc tgacttccac ccggtcaatg gcatacgttg tgcccggcaa tttgaccggt
    11761 gccgcggctt cgcggatcac aaaatctcct tcgcgcgcga caaggcgaat cacccagggc
    11821 gtggcccaag gcagccagat cagcaacagc gccaccgtgc ccaacgccag tttccattcc
    11881 aatgggaagc ggctgctcat tgaactaatc ccgccaaggg tcggcggccc tcatgaatcg
    11941 gaaaatcggt gccgaaggtt tcgttcagca cacggcgcaa ggtatccaaa cccgacaggc
    12001 cttcaatgtt gaacgcccgc ccccggtagt acaccgcatt gaaaatcgaa gccatgtcat
    12061 ccacgggcat ggtcaattca tggcggtaac tgtggtcgga ctgaaccagc acaatacaat
    12121 cgggattggc ggcaatgatt tgctccaaca ccgcaatgat ccgtttggcg atgaattgat
    12181 attgcgccaa ataaaaatca tcaaccttga ccgacccttc ctccttgcgc caagcgccat
    12241 ccggctcaaa cagataaggc gcgtgggggc aggcaatgtg cgcaaacgtg aagctgggcg
    12301 cgcgccggat attcggatcc gccagccacc caaggattcc ttccaccatg cggttgtaga
    12361 aatgcttgtc gtgcggccat atcgtcaaca gcggctccag caccgagttt ttcagcacgc
    12421 tggaaaacac ggtgttttcc aaactgacgc cgcccgcatt gggcggaagt tggaaggtgt
    12481 aatccgccac gaagggggaa ctcacgttga ataagtcgcc cagcatgttc gccacataga
    12541 cggtgtagcc gtgcgcttgg aaaaagcgca tcagtgtgga ttccgcaaaa taggtgtgtt
    12601 ggtcgaggtc cggcgtgttg acatatttct tgatgcgccc atcggccgcc acttggtact
    12661 gcgtgatttc ggccggccat tccatcccga caagtccggc caacacctcc actgtcaaaa
    12721 acgagggatt gtaactgcta ccggataccg agaactgccg ctgtcggaga tattgcgcca
    12781 gttcaaccgg ccgccagttg aaatgtttct gtatttgcaa gaaaggcgca aattcatcca
    12841 gaatgatcag ataaacatct gggcgatcca cccgcgccgc caccggcaca accgaggcag
    12901 gcgcagccgg tgcgcgccga aactgtgtaa cacggcgcca gagcaccggc acggcgccgg
    12961 ctgcattgta tacaatcaga attgcgaaca gccccgccag ccatttccgg taagccagaa
    13021 atctggtttt ccccagacgt gcgcggcgcc ccagcagcaa cgccgcaatc acgccaacgg
    13081 ctagagctac ggggaacaga tgccaatagc gaatcataac ccactgcgcg cgcacggcag
    13141 tttccagtgg gcgccacagc gccgcaatca ggagcgtaag ctgcgcaatc agggcgccga
    13201 cggcaaaacg accagcccac gcgctgaaga aaagccacgc cagcaacgcc accgcgagaa
    13261 aaaacagaat gggcaacttc agttgttctg caccggcctc atgcacattg cggaaataca
    13321 ccagcagcac cggatacagc gcaatcacga gaacgtaaac gtactccttc aggagccgga
    13381 ccattcgact gttttgaaca cgatctatca cgatactcaa caaccgcgcg cggcggccga
    13441 ctctatatcc atttcattat acactggcgc atggtctcca catagcgcga ccatgacaat
    13501 tcttccacca catgacggcg ggcggtggcg cccagtcgct gacaagcctc cggccgggct
    13561 agcaaatcgt tcagcacgac gttcatgctt gcctggctgc ccggttccaa ccagcggata
    13621 ttgagatcat cctgcaggcc atatccgtcg cggatggccc ccacgcgcga taccgccacg
    13681 gccttctcca gcgccatgcc ctgcagcaga gttgtggtgg cgccgctata ggtgttctcc
    13741 ttcaccggca gcactagaac ccgcgcccca gccattacct cctgcacccg cttcactggc
    13801 agttgcacct ggaccgtcac attgggcggc acatccatca acttggccgc cgcatcctgc
    13861 cccaccacca gttgcaccct cacgtgcgga tgctgccggg cataagccag caacaaggga
    13921 aaatcacgct ggggatctgc gcctacgctg accacatccg caatcttcgg cgcgtcaggc
    13981 attggccgcc actgttccac atccacccca aacggcacaa attgcacttt gtcgggcgtg
    14041 cccagccatt cccgcaacca ttcagcttcg gcatagccgt acgccacaaa ttggtccaca
    14101 cgcgacaaca tgttgcggta gcgcgcctcg cgcgccggac taatcttgcg cacggcc
//
LOCUS       DAOPNP010000062        13896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5154_length_13896_cov_7.175060, whole genome shotgun sequence.
ACCESSION   DAOPNP010000062 DAOPNP010000000
VERSION     DAOPNP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5154_length_13896_cov_7.175060"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PKI64_06905"
     CDS             <1..1461
                     /locus_tag="PKI64_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HOE00772.1"
                     /translation="IVGQWTFCSNESRCQPNRAYEKSDGNAGKEARMMATRGVLRGLV
                     LLGLLSAGWGLAQTVLTVDEAATRIGETVTFEGKVMSMASSPEFKATYASFGGAYPFQ
                     KLSVLFAGENEAIVSCDLPRLNGRTVRVTGKVEGGKRGPVVRVTDTNQIEVLAVAVMD
                     SLDSEGEGPAFRKQLKATLRDRFKAGDYASLDAVAAKWRTGKERLLDGTWKIAIFYGT
                     FSEPVLPYPDYFRALEEWQAAFPDSITPRLLQANARVAHAWEARGSGLAITVTEEGWK
                     AFRERLALARSELAALHARRTECPQWFAIMQVVALGQGWSRPEYEALFEEAIRYEPEY
                     LVYYDRKINYLQPKWHGEEGEWVEFVNSLLDRFPGGLGEELYARLAFVYRKDSDQEML
                     RKKGRYFPDMGFRWEPMKAGFERMRARFPKSSWILNGYAIFAGKAGDWETTRHLLLEL
                     GDNCDMNIWLTWNNVALARMWADGKGLSDSYFILFR"
     gene            1729..2268
                     /locus_tag="PKI64_06910"
     CDS             1729..2268
                     /locus_tag="PKI64_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOE00773.1"
                     /translation="MSDFVTAHNLWAPWRMEFFRAPKEAGCFLCRIIRSGARADAQNL
                     VIHRGRKCLLLMNRYPYSNGHLMVVPRRHVGPLGELTAAERAELLDLACLGQRLLAHV
                     ARPHGFNLGINEGVAAGAGLQDHLHLHVVPRWTGDTNFMPLLGQVRVVPQALAELWAA
                     LRTALAQEKPRRGTRKGGK"
     gene            2265..3239
                     /locus_tag="PKI64_06915"
     CDS             2265..3239
                     /locus_tag="PKI64_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE00774.1"
                     /translation="MKEETIHFDNGREAQEIVGLREEHLPGLEQSFAVKAVARDTWLK
                     LSGAAESVAAAEAFVDGLRRARMAGMTLTRQAVDYARAAYVAGQGAEFDRLTSLRVDV
                     APGRAPVFPRTFGQAQFLTTIRNNELTFGLGPAGTGKTWLAMAMAVSALLKNEVARII
                     LTRPAVEAGEALGFLPGDLQQKILPYLRPLYDALHDMMPPENVPELVERGVIEVAPLA
                     YMRGRTLNRAFVILDEAQNTTREQMLMFLTRLGFGSKAVVNGDLTQVDLPAHKMPGLL
                     EAEYLLRNVAGVGVVRLTAADVVRHPLVQRIIEAYQGREKGGGNGGTN"
     gene            3241..5508
                     /locus_tag="PKI64_06920"
     CDS             3241..5508
                     /locus_tag="PKI64_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOE00775.1"
                     /translation="MAMGNAKKQAVRVAAAQDEPLRVRGWRMAGNVALLLALWLIGVM
                     CIQVVGRRGYASLAPGQRAPATVVAAYDFDCVNLPVTELLRQQAADAVTPVFALPSGS
                     LQAARRGMEKALAEEAGPLRHASEDEKLLAALRDSLTDIWLEGILAPAEQDSAPHGPM
                     PHSQIDILHSLENGTPSVRTVQRDDLLTPEKARAAFAEAAQRRLGKAGGDISLHELED
                     LVKPILRPNLEYDEAATQARRLEAQNRVEPVMITVREGTTLMEEGSTVTPQTVEYISK
                     HNRKLVEKESPTDRRLKRGGDAALMLIVLVICTGWLRSTQPGVYTRSRRKGLLILLAL
                     LALLQASLFRWLSTDLYWIPPWLAPFAITITLPSMLAALMMGPAAALAVGLWSGLATA
                     LIFERSFELLLLGVGGSVLAVALLRNVRKRSQVMRAGLAVGLLEGVVALALAALYLYM
                     KKTFLIQVATGIGSGVLSALLALLILPFLEWMFQHTTDITLLELTDMGHPLLQRLAIE
                     APGTFHHSLMVGALAQAAAARIQANSLLVLVCAYFHDIGKLTKPEFFVENQRGGENPH
                     DGLAPSMSALVIQSHVKEGLTLAKRYKLPLVVSDAITMHHGTSLTSYFYQVARRALKD
                     AGMAEDGGLEHSFRYDGPRPTSREQAIVMLADTVEAASRSLEKPTPNRIEEMVTALFR
                     DKLLDGQLDTSPLTLAELHQIQESFVSGLTNILHGRNPYPRENPSAQSATGAVPQPPA
                     AAPAGAGTGSLGVSG"
     gene            5408..5866
                     /gene="ybeY"
                     /locus_tag="PKI64_06925"
     CDS             5408..5866
                     /gene="ybeY"
                     /locus_tag="PKI64_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOE00776.1"
                     /translation="MKIHLLNQQRALSLNLPPLRRLARALAAWAFPADPPFAELTILL
                     TDDAAMPDYKAGCFGVRQQTDVVAQAYAAMPGMSAATAELVINAERARQEGARRAGGA
                     ARELALYLAHGMDHLAGATDDTPARRQAMRRRENTWLEATADLWSAIVRP"
     gene            5863..6600
                     /gene="recO"
                     /locus_tag="PKI64_06930"
     CDS             5863..6600
                     /gene="recO"
                     /locus_tag="PKI64_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOE00777.1"
                     /translation="MIIKDEAIALRTYPYGNTSQIIVWLSQTHGKIATLAKGSQRTRS
                     GLLGQYDLFYTCEVLFYAREQRNLHILKKCCPLVPRVALRHDWRACAVASYAASAVDQ
                     LAPFGPAMSGLYDLLARTLDSLAGRTPGPAFLPRFELLLLHELGLAPDWRACAKCRQD
                     LRERGGRWDVAHGALRCVECAEDSGLEVSAAALRAIETMQGEALPAALPERVFREIQS
                     LAGSLLEHQADCSTVARTLAFQVLAAG"
     gene            complement(6579..7400)
                     /locus_tag="PKI64_06935"
     CDS             complement(6579..7400)
                     /locus_tag="PKI64_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE00778.1"
                     /translation="MPSVPQPITSLQNPGVKEIVKLRQRSHRDENALLIVEGYRETKR
                     ALDNGIPIRKLFFCPALYQGKNEPAIVARCAELNVPLWECSEAVFRKIAYRDRPEGLL
                     ALCPQIRRTLADLRLPKNPFIVVAEHIEKPGNLGTMLRTADAAGVDAFIVCDRCTDIN
                     NPNVVRASIGTLFTVQVAEASTPETIAWLATHQIRVVAATPHATQLYTAVDMTKPVAI
                     ILGAEQFGLSDTWLAKATDQVRIPMLGQADSLNVAAAATILLYEVVRQRQPAAST"
     gene            complement(7403..8413)
                     /locus_tag="PKI64_06940"
     CDS             complement(7403..8413)
                     /locus_tag="PKI64_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00779.1"
                     /translation="MSTQRILSVMVVTLNEARHLPSLHDALARLHLPPTWSLETILVD
                     GGSRDDTVTIARDLGFTECLVHPGASIPVCRNHALRAARGNALAFLDGDCLPSRNWLE
                     VAAPWLVKPGPVLLGYPVRPSVTGNWIQRSWHAHWHHKNPAAAAGGQREPVRHEAFRL
                     LTTRNMLFNRALLDVIPTFDESLTTGEDTDFAFRAAQAGVEVVALPDLEVTHLGEPAT
                     LRQYFRQQLWHANRRAYAGILKSSGGRAGANAIFFTLSFLLALLAALASVALAFAVSP
                     WALLGVIPLLAVTAIPAALIADRARSPLLLPQLCVLYFLYGLARSADLIGLSPRKKSW
                     KS"
     gene            complement(8410..9147)
                     /locus_tag="PKI64_06945"
     CDS             complement(8410..9147)
                     /locus_tag="PKI64_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE00780.1"
                     /translation="MNALTNIRIVLVNPIYGGNVGSVCRAMKNMGLSQLVLVNPRPGM
                     DMEDAAAMACHAGDVLQNRAVFDTTAEAVADCGLVAGTSNRGGLYRDHSKTPREWAPR
                     LLAAALDTPVALLFGSEDNGLLLDDLALCTQFIRIPSSDSYKSLNLAMSVMICAYELF
                     VASNTFEPPQERSAECPSAMREAMFAKWRKALLDIGFMTPDTADHMMLGIRRILSRGT
                     LTEKDVQILLGMASQTQWAANQLPRKP"
     gene            complement(9226..10908)
                     /locus_tag="PKI64_06950"
     CDS             complement(9226..10908)
                     /locus_tag="PKI64_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00781.1"
                     /translation="MADAPKSKTLLGVRYDHYVLPDGADLYVTQHGRPFLQHLQPENW
                     FEREWFRRSREKLQGTSTVYKVRTKPVAGQSKDLVVKWCRVGEAVPLDTFTLTKFSDA
                     EFNTPYEEFSLVMEMRATADPGTIRTHKPLAIFVPSKRLELWQTGRSRSKIALKKSKF
                     RDVELDIYRQYILIYEWIKGAACTEPAAIAAALESGYPDAQSLARDMLQRSIADMWQA
                     GYRVLDVKPEHVIVRPRRGGGLLRDTSGRPAYALVDFELLARTPEHEEQVKGARRQTY
                     LERQRDRFVAPADPALFPEHLRPMRILGVDYVYGDCASTRGKLWVVGRDPLLFDYFQP
                     ERWRRTPRSSLSDSAQVYHTKTKDGIELVWKVAHVGDPVDASQNPPALVEHGYNSPFE
                     EFAYAFQLTAAGVPTTYPRAIYMLGHQSTLPPQFLDQRRYVSHQDILQPDNTPVLQRE
                     RNYICIWGYWNGLDEVLAREDRQRPHCRGVNAAQAFTQGLISSAEHAACVVKMARLLA
                     AAGFESTNPRDTHFLLTLMPDGHLMRDPDGLPAVRLCNFEFLRPLAATKPTT"
     gene            complement(10998..12410)
                     /locus_tag="PKI64_06955"
     CDS             complement(10998..12410)
                     /locus_tag="PKI64_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00782.1"
                     /translation="MRHYFSDIFLRKAVIALSGLLMLAGGAAAREFTVNTISDEKGNA
                     RTPVISDTGLVAWQAYSLHDGDTPVHQRVDVLSAPPNTRRSDIIIWQDGKTNNLTATD
                     TRLTGRCEQPVVGGNAVAFLAWFKNETDLGMPFTLSIPDKTDEMKQMESDYPTLFDPP
                     KPPPVSTVEAEAGEAAPPPEPETPEAAATNGLNENLQRQMWRDSGQAADIVYYNTARG
                     QLERLTSGNRHYCSPVVSDAGVAFQCARGWPYGYEMVAWPASATNLVQLTTNYYYVLH
                     PDLHGNELVFQGWDGNDYEIFRYRFDTGQMEQITNNSFDDTAPVVWDGRIAWVGYPTI
                     TAEIFYWADGALRKISERTEDNTAPSIWEGQVVWQAYDDTDMEIYYFNGRRTIKLTSN
                     TWDDLAPQIRDGLITWMSYVDNWDSEIMAFDLSDNIAVQLTNNEFEDSYPQTAGGKIV
                     WQTIGAEGSFIQMAVPAAAP"
     gene            complement(12417..13709)
                     /locus_tag="PKI64_06960"
     CDS             complement(12417..13709)
                     /locus_tag="PKI64_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00783.1"
                     /translation="MRKIYKRSLALLFSVVLIVGLVVGWRAWNVVNTAYGGFQHLRYL
                     LESGETVEGAPADDVLSDSYLLSLFGDNPELVDRLKMVIDLGMATDANLKLGNVSAMV
                     VTYQLDAASNVTDTAIYAVGGFPDPKSRRLGFHSTGYFKEELDADLWHTGNSLMNLLG
                     RDIIVFCEEEKAEAHMNLLYGLLNGDIVPLAKRIVEAPVHYAIVFPRPKELAPPNLKN
                     HLQTVMFKGVMGPDTGKTEMMLVSQTMAKAYQVYTIVKDMRTLAGITFHDKFSGYIKQ
                     MPWGTMNDTWWAVEYNSLIESSELLHDQILVIGRVEYDRPKNNAILKTIERAGRDLAM
                     QKSFSLASELPWEFAYQAKDNPSGGYWSEEHRWGDQWPLGEEGVETPGSRAAKAERER
                     LRAEQEAARLQAKERGETGQPPSSPAPAAPSTTPAATL"
BASE COUNT         2648 a         3856 c         4589 g         2803 t
ORIGIN      
        1 attgtggggc agtggacttt ctgctcaaat gaatcacggt gtcaaccgaa cagggcttat
       61 gaaaagtccg acgggaacgc aggcaaggag gcgcgcatga tggcgacaag aggggtattg
      121 agggggctgg ttcttttggg cctgttgtcg gcggggtggg gattggcgca gacggtgctg
      181 accgtggatg aggccgcaac gcggatcggc gagacggtca cctttgaagg caaggtcatg
      241 agcatggcat cttcgccgga gttcaaggcc acctatgcca gcttcggggg ggcgtatccg
      301 tttcagaagc tttcggtact ttttgccggc gagaacgaag cgatcgtttc gtgcgatctc
      361 ccgcggctca acggtcggac cgtgcgcgtg accggcaagg tcgagggcgg gaagaggggt
      421 cccgttgtgc gcgtgacgga cacgaaccag atcgaggtgc tggcggtcgc ggtgatggat
      481 tcgctggatt ccgagggcga ggggccggcc tttcgcaagc aactgaaggc cacgctgcgc
      541 gaccggttca aggccggtga ttatgcgtcg ctggatgcgg ttgcggcgaa atggcggacg
      601 ggcaaggagc gcctactcga cggcacctgg aagattgcga tcttttacgg gacgttttcc
      661 gagccggttc tgccgtatcc ggactatttc cgggcgctgg aggagtggca ggcggccttt
      721 cctgattcca ttacgccccg gctgcttcag gcgaatgcgc gggtggccca tgcgtgggaa
      781 gcccgcggca gcggtctcgc catcacggtc accgaagaag gctggaaggc gttccgggaa
      841 cgcctggcgc tggcccggag cgaactggcc gccctgcatg cgcgccggac ggaatgtccc
      901 cagtggtttg cgatcatgca ggtcgttgcg ctgggacagg gctggagccg cccggaatac
      961 gaagccttgt tcgaagaggc gatccgctac gagccggaat atctggtgta ttacgaccgg
     1021 aagatcaatt atctgcaacc gaaatggcat ggtgaggaag gcgagtgggt ggagttcgtc
     1081 aactcgcttc tggatcgctt tcccggtggc ctgggagagg agctgtacgc ccggctcgcc
     1141 ttcgtgtatc gcaaggactc cgatcaggag atgctccgga aaaaggggcg gtattttccc
     1201 gacatggggt tccggtggga acccatgaaa gccgggtttg agcggatgcg cgcccgcttt
     1261 ccgaagtcct cgtggatact caacggctat gccatttttg ccggcaaggc gggcgattgg
     1321 gaaacgaccc ggcatctgct gctcgaactg ggcgacaact gcgacatgaa catctggctg
     1381 acgtggaaca acgtcgccct cgcccggatg tgggctgatg gcaaggggct ttccgacagc
     1441 tattttatct tgttccgctg atcccgaaat ctgcggcggc gagagatcat gagtccattt
     1501 ttaaggggac agccgggaaa atcagaacgt agagggggca gtttgtgctg ccgcctgccg
     1561 ccttctgggg catcttcatt cgcttcattt ccccggttgc cattctcttc gccttcctcc
     1621 acaccatcgg ctgggacaac ttaaggtaag cggggttggg ccggggcgcg cggcacaaaa
     1681 cgggattttg ggctgtgcgc cggcagggga tggggtattc tattggccat gtcggatttt
     1741 gtgaccgcac ataatttatg ggctccatgg cgcatggagt ttttccgtgc gccgaaggag
     1801 gccggttgct ttctgtgccg gattatccgg tcaggggcgc gggccgatgc ccagaatcta
     1861 gtgatccacc gcgggcgcaa gtgcctgctg ctgatgaacc gctatccgta tagcaacgga
     1921 catctgatgg tggtgccgcg ccgccatgtc ggcccgctgg gcgaattgac tgcagccgag
     1981 cgcgccgagt tgttggattt ggcatgcctc ggtcagcggc ttctggcgca cgtggcccgc
     2041 ccacacggct tcaacctggg catcaatgaa ggcgttgcag ccggcgccgg cttgcaagat
     2101 cacctgcatc tgcatgtagt accgcggtgg acgggcgaca cgaatttcat gccgctattg
     2161 gggcaggtgc gggtggtgcc gcaggccttg gcggaactgt gggccgcctt gcgcacggca
     2221 ttggcgcaag aaaaaccgcg ccgcgggaca agaaaaggcg gtaaatgaag gaagagacca
     2281 tccattttga caacgggcgc gaggcgcaag agattgtcgg cttgcgtgag gaacacctgc
     2341 ccggtctgga gcagtccttc gccgtcaagg ccgttgcgcg ggatacgtgg ctgaaactct
     2401 ccggcgcggc ggagtcggtg gccgcggccg aagcctttgt ggatggcctg cgccgcgccc
     2461 gaatggctgg gatgacgttg acccggcagg cggtggacta tgcccgtgca gcttatgtgg
     2521 cagggcaggg cgcagaattt gaccggctga ccagtttgcg ggttgatgtt gcccccggcc
     2581 gcgcgccggt gtttccccgc acatttggac aggcccaatt tttgaccacc atccgtaaca
     2641 acgaattgac gtttggtttg ggccccgcgg gcaccggcaa aacatggctg gccatggcca
     2701 tggcggtctc ggcgctgctc aaaaatgagg tggcgcggat cattctgacc cggcctgccg
     2761 tggaagcggg cgaggcactc ggttttctgc cgggcgattt gcagcagaaa atcctgccct
     2821 atttgcgacc gctttatgat gctttgcacg acatgatgcc gccggaaaac gtgccggaat
     2881 tggtggaacg cggcgtgatc gaagtggccc ccttggccta tatgcgggga cgtaccctca
     2941 accgcgcgtt tgtgattttg gacgaagcgc aaaacaccac gcgggaacag atgctgatgt
     3001 tcctcaccag gttgggtttc ggttccaagg ccgtggtcaa tggcgattta acgcaagtgg
     3061 atttgcccgc acacaaaatg ccgggactgt tggaagccga atatctgctg cgaaatgtgg
     3121 ctggggtggg ggtggtgcgc ctgacggccg ccgatgtggt gcgccacccg ctggtgcagc
     3181 ggatcattga agcctatcaa ggccgcgaaa agggtggggg gaacggagga acaaactgac
     3241 atggctatgg gaaatgccaa aaaacaagcg gtgcgagtgg ccgcggccca agatgagccc
     3301 ttgcgcgtac ggggttggcg catggccggc aatgtggcgc tgttgctcgc gctgtggctc
     3361 atcggtgtga tgtgcattca ggtggtcggc cgacggggct atgccagcct tgcgcccggc
     3421 cagcgcgcgc ccgccaccgt ggtggcggcg tacgattttg attgtgtcaa tttgccggtc
     3481 accgagctgc tccggcagca ggccgccgat gcagtgacgc cggtgtttgc cctgccctcc
     3541 ggctcactgc aggccgcgcg gcgtggcatg gaaaaggcgc tggcggagga ggccggcccc
     3601 ctgcgccacg ccagcgaaga cgaaaaactg ttggccgcac tgcgggacag cctgacggat
     3661 atttggctgg agggcattct ggcccctgcg gagcaagaca gtgcccccca cggtcccatg
     3721 ccgcactcgc agattgatat tttgcattcc ttggaaaacg ggacgccaag tgtgcggacc
     3781 gtccagcggg atgacttgct gactccggaa aaagcgcgcg cggcctttgc ggaagccgca
     3841 caacgacggc tgggcaaagc cggcggcgac atatcgctgc acgagctgga agacttggtg
     3901 aagccgattt tgcggccgaa tctggaatat gacgaagccg ccacgcaagc ccgccggctt
     3961 gaagcacaaa accgcgtgga gccggtgatg atcaccgtgc gcgagggcac cacgctcatg
     4021 gaagagggtt ccacagtcac gccgcaaaca gtggaataca tcagcaagca caaccgcaag
     4081 ctggtggaaa aagaaagccc gaccgaccgg cgtttgaagc gggggggcga tgccgctttg
     4141 atgctgattg tgctggtgat ttgcaccggc tggctgcgga gcacgcagcc gggggtctac
     4201 acccgctcgc ggcgcaaggg gctgctcatt ctgctggcct tgctggccct gttgcaggcc
     4261 agccttttcc gctggctgtc caccgacctt tactggattc cgccctggtt ggcgccattt
     4321 gccatcacca tcacgctgcc ttccatgctg gccgccctga tgatggggcc ggcagcggct
     4381 ctagccgttg gcttgtggag cggactggcc acggccctga tctttgagcg gagcttcgag
     4441 ttgcttcttc tgggcgtggg cggctccgta ctggcggtgg ccttgctgcg caatgtacgc
     4501 aaacggtcgc aagtcatgcg ggccggcttg gccgtaggtc ttttggaagg cgtggtggcg
     4561 ctggcgttgg ccgcgcttta cttgtacatg aagaaaacct tcctgattca agtggccact
     4621 ggcatcggca gcggagtgtt atcggccttg ctggcgttgt tgatcttacc gttcctggaa
     4681 tggatgttcc aacacaccac ggatatcaca ctgctagagc tgaccgatat gggtcacccc
     4741 ttgctgcagc ggttggccat tgaggcgccg gggaccttcc atcacagctt gatggtcggc
     4801 gcgctggcgc aagctgccgc cgcacgcata caagccaaca gccttttggt actggtgtgc
     4861 gcttattttc atgacatcgg caaactgacc aaaccggagt tctttgtgga aaaccagcgc
     4921 ggcggtgaaa acccgcatga tgggttggcg ccgagcatga gcgcgctggt gattcaatcg
     4981 cacgtcaagg aaggacttac tttggccaag cggtacaaac tgcccttggt ggtcagcgac
     5041 gccattacca tgcaccacgg cacctcgctg acttcctatt tctatcaagt ggcgcgtcgc
     5101 gcgctgaaag acgcggggat ggccgaagat ggcgggttgg agcacagctt ccgctatgac
     5161 ggcccgcgcc cgacgtcgcg cgagcaggcg attgtcatgc tggcggacac cgtggaggcg
     5221 gcttcgcgct cgctggaaaa accgacgccc aaccggatcg aagaaatggt gactgcactt
     5281 ttccgcgaca agctgctgga cggccaactg gacacctcgc cattgacgtt ggctgagttg
     5341 caccagattc aagagtcgtt cgtttcgggc ctgaccaaca tcctccatgg aaggaatcca
     5401 tatccccgtg aaaatccatc tgctcaatca gcaacgggcg ctgtccctca acctcccgcc
     5461 gctgcgccgg ctggcgcggg cactggcagc ctgggcgttt ccggctgatc caccgttcgc
     5521 cgagctgacg atcctgctga ccgacgatgc ggccatgccg gattacaagg ctggttgttt
     5581 cggcgtacgt cagcaaacgg atgtggtagc gcaggcctat gcggccatgc cgggcatgag
     5641 cgcggccacc gccgagttgg tgatcaatgc tgagcgtgcc cgccaagagg gcgcgcgccg
     5701 cgccggcggt gccgcgcgcg agctggcgct atatcttgcg cacggcatgg atcatttggc
     5761 gggcgccacc gatgacaccc ccgcgcgccg ccaagcgatg cgccggcgcg aaaacacatg
     5821 gctggaggca actgcagacc tttggtccgc cattgttcgg ccatgatcat caaggatgaa
     5881 gctattgccc tgcgcacgta tccttatggc aatacgtcgc aaattatcgt gtggctgtcg
     5941 caaacccacg gcaaaatcgc cacgctggcc aaaggcagcc agcggacacg cagcggcctg
     6001 ctggggcaat atgatttgtt ttacacctgc gaggtgctgt tctacgcccg cgagcagcgc
     6061 aatttgcata ttctgaaaaa atgctgcccg ttggtgccgc gtgtggcatt gcgccatgac
     6121 tggcgggcgt gcgcggtggc ttcctatgcg gccagtgccg tggatcaact ggcgccattc
     6181 ggtccggcca tgagtggact ctatgacttg ctggcgcgga cgttggactc gctggcggga
     6241 cgtacgccgg gaccggcctt tttgccacga tttgaactgc tgttattgca tgagctgggc
     6301 ttggcgcccg attggcgggc ctgcgcaaaa tgccgccaag atttgcgcga gcgggggggg
     6361 cgttgggatg tggctcacgg cgcgttgcgg tgcgtggaat gcgcggagga ttccggcctg
     6421 gaggtttccg ccgcggcatt gcgggctatc gaaaccatgc agggcgaagc gctgcccgcg
     6481 gcattgcccg agcgggtatt cagggaaatc caatcgctgg ccggcagttt gttggagcac
     6541 caggccgatt gttccaccgt ggcgcggacg ctggcctttc aagtgctggc agccggttga
     6601 cgctggcgaa ccacttcata cagcaggatt gtggcggcag ccgccacgtt gagcgagtcg
     6661 gcctgaccca gcatgggaat gcggacctga tccgtcgctt tggcgagcca ggtgtcactc
     6721 aaaccgaatt gctccgcgcc aaggatgatg gccacaggct tggtcatatc caccgcggta
     6781 tagagctggg tggcgtgagg cgtggcggcc accacgcgaa tctggtgcgt ggccagccac
     6841 gcgatggttt ccggcgtgct ggcctcggcc acttggactg tgaagagcgt gccgatgctg
     6901 gcgcggacca cgttgggatt attgatgtcg gtgcagcggt cgcaaacgat gaacgcatcc
     6961 acgccggcgg catccgcggt gcggagcatg gtgccgagat tgccgggttt ttcaatgtgc
     7021 tccgccacca cgatgaacgg atttttcggg agccgtaaat cggcgagtgt acggcgaatt
     7081 tgcgggcaaa gcgccaagag gccttcaggt cggtcccgat aggcaatttt gcgaaaaacc
     7141 gcttcgctgc attcccagag cggcacattg agttctgcgc aacgcgccac aatggccggc
     7201 tcattcttcc cctgatagag cgcggggcaa aaaaatagtt tgcggatggg aatcccgttg
     7261 tcgagcgcac gcttggtttc acggtagcct tccacaatca gcagggcatt ttcgtcgcgg
     7321 tgcgaccgct gccgcagttt gacgatttcc ttgacgccgg gattctggag actggtgatg
     7381 ggctggggca cgctgggcat ggttaggact tccaggattt tttgcgtggc gagaggccaa
     7441 tcaagtctgc gctgcgcgcg aggccataga ggaaatagag cacacacaat tgggggagga
     7501 ggagcggcga gcgggcccgg tcggcgatca atgcggcggg aatggcggtg accgccagta
     7561 gggggattac gcccagcagg gcccatggcg aaacggcaaa cgccaatgcc acgctggcga
     7621 gggccgcgag cagcgcgagc aaaaaggaca gggtgaaaaa aatggcattg gcgccggcac
     7681 gcccgccgga gcttttgaga ataccggcat aggcccggcg attcgcgtgc cacagttgct
     7741 ggcggaagta ttgccgcaga gtggccggtt cgccaaggtg ggtgacttcc aaatccggca
     7801 aggccacaac ttccacgccg gcttgtgcgg cgcggaaggc gaagtcggtg tcctcgccag
     7861 tggtcaagga ttcatcgaaa gtcggaatca cgtcaagcaa ggcgcggttg aagagcatgt
     7921 tgcgcgtggt gagcagccga aaggcctcat ggcgtacggg ttcacgctgg ccacccgcgg
     7981 cggccgcagg gtttttgtgg tgccagtggg cgtgccacga gcgctggatc cagttgccgg
     8041 tcacggaagg acgcaccgga taaccgagca acacggggcc gggtttgacc agccacgggg
     8101 cggccacttc cagccagttg cggctgggga ggcaatcgcc atccagaaaa gccagcgcgt
     8161 taccgcgcgc ggcacgcaag gcgtgattgc ggcaaaccgg gatggaagcg ccgggatgga
     8221 caagacattc cgtaaaaccc aggtcacggg cgatggtcac ggtatcatca cggctaccgc
     8281 catccaccag gatagtttcc aggctccacg ttggcggcag gtgcagacga gcgagggcat
     8341 catgcaaact gggcaaatgc cgcgcttcgt tgagcgtgac caccatgacg gataaaatgc
     8401 gctgcgtgct catggcttgc ggggaagttg attggctgcc cactgggttt gcgaggccat
     8461 gcccagcagg atttgcacat ccttttcggt gagggtgccg cgcgaaagaa tccggcggat
     8521 gccgagcatc atgtggtcgg cggtgtctgg cgtcatgaag ccgatatcga gcagcgcctt
     8581 gcgccatttg gcaaacatgg cctcacgcat cgcggaggga cattctgcgg aacgctcctg
     8641 gggtggctca aatgtgttgc tggccacaaa caactcatag gcgcaaatca tcacagacat
     8701 ggccaaatta agggatttgt aggagtcgga ggaggggatg cgaatgaatt gggtgcagag
     8761 ggccagatca tccagcagca ggccgttatc ctcggaaccg aacagaagcg ccacgggggt
     8821 atcgagtgcg gccgcgagca ggcgcggagc ccattcccgt ggcgttttgg aatgatcgcg
     8881 gtacagtccg ccgcgattag aggtgccggc caccagtccg caatctgcga cggcttccgc
     8941 ggtggtgtca aacaccgcgc ggttttggag gacatccccc gcatggcagg ccatggctgc
     9001 ggcatcttcc atgtccattc cagggcgcgg attgaccaac accaattgcg acagtcccat
     9061 gtttttcatg gcgcggcaca ccgagccgac gttgccgccg taaatcgggt tgaccaggac
     9121 aatgcggatg ttggtcagcg cattcattgt ttggtgagca tagcgtgtga cgggtaaaat
     9181 gcaattctcg ccgcggctcg ccgggcggat cgcgtttcgc cggatttatg tggttggttt
     9241 ggtggccgct agggggcgca ggaattcgaa attgcataag cgcacggcgg ggaggccgtc
     9301 ggggtcgcgc atcagatggc cgtcgggcat gagagtcaga agaaagtggg tgtcgcgcgg
     9361 gttggtgctt tcaaatccgg ccgccgccag cagccgggcc attttgacca cgcaggccgc
     9421 gtgttccgcg gaactgatca gtccttgcgt gaaagcttgc gcggcattta ctcctcggca
     9481 atgcgggcgc tgacgatctt cgcgcgccaa cacttcgtcc agcccgttcc agtatcccca
     9541 gatgcaaata tagttccgct cccgctgcag cacgggagtg ttgtcgggct gcaaaatatc
     9601 ctgatggctg acgtagcgtc gttggtcgag aaactggggg gggagggtgg actgatgacc
     9661 cagcatataa atggcgcgcg ggtaggtggt gggcacaccg gcggccgtca actgaaaggc
     9721 gtaggcaaac tcctcaaagg gactgttata accgtgttcg acaagagccg gcgggttttg
     9781 gctggcatcc acggggtcgc cgacatgcgc gaccttccaa accagctcga tgccatcctt
     9841 ggttttggtg tgatatacct gcgcgctatc cgaaagactg gagcgcggag tgcgccgcca
     9901 gcgttccggt tggaagtaat caaatagcag tggatcgcgc cccaccaccc agagtttgcc
     9961 acgggtgctg gcacaatcgc catagacata gtccacgccc aaaatgcgca tggggcgcag
    10021 gtgttctgga aaaagagccg gatcggcggg cgcgacaaaa cggtcgcgct gccgctccag
    10081 ataggtttgc cgccgagcgc ctttgacttg ctcttcgtgc tccggggtgc gggccagtag
    10141 ttcaaagtcc acgagtgcgt aggcgggccg gccgctggtg tcacgcaaca ggccaccacc
    10201 gcggcggggg cgcacgatga cgtgctccgg cttcacgtcc agcacccggt agccggcttg
    10261 ccacatgtct gcaatggagc gctggagcat gtcgcgcgcc agcgattggg catccggata
    10321 gccggactcc aaggcggcgg caatggctgc gggctcggtg caggccgcgc ctttgatcca
    10381 ttcgtaaatc agaatgtact gacggtagat atccagctcc acatcgcgga acttggattt
    10441 tttgagggca atcttggagc gcgagcggcc ggtttgccag agttcaaggc gtttcgacgg
    10501 tacaaaaatg gcgagcggtt tatgtgtgcg gatggtcccg ggatcggccg tggcacgcat
    10561 ttccatgacc agcgaaaact cttcataggg cgtgttgaat tcggcatcgc tgaacttggt
    10621 cagggtaaag gtgtcgaggg ggacagcttc gccgacccgg caccatttca ccacaaggtc
    10681 tttgctttgg ccggcgacgg gcttggtgcg cactttatac accgtgctgg tgccctgcag
    10741 tttttcgcgg ctgcggcgaa accattcacg ttcgaaccag ttttctggtt gcaggtgctg
    10801 gagaaaaggg cggccgtgct gcgtcacata gagatccgcg ccatcgggca ggacataatg
    10861 gtcatagcgc actccaagca gggttttgct tttgggggca tcagccatga ggcacacatc
    10921 cgggcggaaa gggttgcgcc gcggaccgcg cgcggtcggt ggcgaaaaac ggcagcaagg
    10981 cgtcaggcac gtggcggcta gggggcagcg gccggcacgg ccatttggat aaacgagcct
    11041 tccgcgccga tggtttgcca cacgatttta ccgccggcag tttggggata ggagtcttca
    11101 aattcgttgt tggtgagctg cacggcgatg ttgtcgctga gatcgaacgc cataatttcg
    11161 gagtcccagt tgtctacgta gctcatccag gtgatcagtc cgtcgcgaat ttgcggcgcc
    11221 aagtcatccc acgtattgct ggtcaacttg atggtgcggc ggccgttgaa ataataaatt
    11281 tccatgtcgg tgtcgtcgta ggcctgccag accacttggc cttcccaaat agatggcgcg
    11341 gtgttgtctt ccgtgcgctc gctgatcttg cgcagtgcgc cgtcagccca atagaaaatt
    11401 tcggcggtga tggtgggata gcccacccaa gcgatacgtc cgtcccaaac tacgggggcg
    11461 gtgtcgtcaa aggagttgtt ggtgatctgc tccatctggc ccgtgtcaaa gcggtagcgg
    11521 aagatttcgt agtcgttgcc atcccagcct tgaaagacca attcgttgcc gtgcaaatcg
    11581 gggtgcagga cgtagtaata gttggtggtc aattgcacca gattggtcgc gctggcgggc
    11641 catgccacca tttcgtaacc gtagggccag ccgcgcgcgc attgaaacgc cacgccggca
    11701 tccgatacca cgggcgagca ataatgccgg ttgccggaag tgagacgttc aagttgtcca
    11761 cgggcagtgt tgtagtagac aatgtcggcc gcttgtcctg aatcgcgcca catttggcgt
    11821 tgcaggttct cgttcagccc gttggtggcg gcggcttccg gtgtttccgg ttccggcggg
    11881 ggagcggcct cacctgcttc cgcctcaacc gtggaaacgg gcggcggttt cggcgggtcg
    11941 aagagggtgg ggtagtccga ctccatttgt ttcatttcgt cggttttatc cggaatactc
    12001 agggtgaacg gcatgcccaa atcggtctca tttttgaacc acgccaaaaa ggccacagcg
    12061 ttaccgccga ctaccggctg ttcgcagcgc ccggtgagcc gcgtgtccgt ggcagtcagg
    12121 ttattggttt tgccatcctg ccaaattatg atgtcggaac gccgggtgtt gggcggggcc
    12181 gaaaggacat ccacgcgctg gtgcacgggc gtgtcgccat cgtggaggga gtaggcctgc
    12241 caagccacca agccggtgtc gctaatcacc ggggtgcgcg cattgccttt ttcatccgaa
    12301 atggtgttga ccgtgaactc gcgcgcggca gcgccaccag ccaacatcag caggccggat
    12361 aaagcgataa cagccttgcg caggaaaatg tcagaaaagt agtggcgcat gaaagtttac
    12421 agggtcgcgg cgggtgtggt ggaaggagcc gctggcgcgg gggaagatgg gggttggccg
    12481 gtttcgcctc tttccttggc ttgcaggcgg gcagcttcct gttcggcgcg gaggcgctcg
    12541 cgttcagcct tcgctgcacg cgagccgggc gtctcgacgc cttcttcgcc gaggggccat
    12601 tggtcacccc agcggtgttc ctcgctccag tagccacctg acggattgtc cttggcctga
    12661 tacgcaaact cccacggcaa ctcgctggcg agagagaatg atttttgcat ggccaaatcg
    12721 cgtccggcgc gttcgattgt cttcaaaatg gcattgtttt tggggcggtc gtattccacg
    12781 cgtccgatca ccaggatctg gtcgtggagc agttcgctgg attcaatcaa gctattatac
    12841 tccaccgccc accatgtgtc gttcatggtg ccccacggca tttgcttgat gtagccgcta
    12901 aatttatcgt ggaaggtaat accggccaag gtgcgcatgt ccttgacgat ggtgtagacc
    12961 tgataggcct tggccatggt ctggctcacg agcatcattt cggttttgcc ggtatcgggc
    13021 cccatgacgc ccttgaacat gaccgtctgc aagtggtttt tgaggttggg tggcgccagt
    13081 tccttgggcc gcgggaaaac gatggcgtag tgaaccggcg cctccacgat gcgtttggcc
    13141 agtggcacga tatcgccatt gagcaagccg tagagcaggt tcatgtgcgc ctcggccttt
    13201 tcttcctcgc aaaaaacaat gatgtcgcgc ccgagcagat tcatgagcga gttgccggtg
    13261 tgccagagat cggcatccag ttcttccttg aaatagccgg tggagtggaa gcccagccgc
    13321 cggctcttgg gatcggggaa cccgcccacg gcatagatgg cggtgtccgt gacgttgctg
    13381 gccgcatcca gttggtaggt gacgaccatg gccgatacgt tgcccagctt gaggttggca
    13441 tcggtggcca tgccgaggtc aatgaccatt ttcagccggt ccaccagctc cgggttgtcg
    13501 ccaaatagtg agaggaggta gctgtcggag agcacgtcat cggccggcgc cccctcaacg
    13561 gtttcaccgg attccagcaa atagcgcaaa tgctggaagc cgccatacgc ggtgttgacc
    13621 acgttccacg cgcgccagcc cacaaccaag ccgacgatca gcaccacgct gaacaataac
    13681 gctaaactac gcttatatat tttgcgcatg actcgaccag ctcgtttagg atgctcctac
    13741 actcaaatat agggcaggcc ccataacggc acaagtacaa aaggccagaa gttggccggc
    13801 ttttttgagc aggtcaattg acaaagtaaa tgcacccccc ggggtctctt gggggtgcgt
    13861 ttagtctacc aaatggcggg ggtgcattta gtctgc
//
LOCUS       DAOPNP010000063        13820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5186_length_13820_cov_6.986778, whole genome shotgun sequence.
ACCESSION   DAOPNP010000063 DAOPNP010000000
VERSION     DAOPNP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13820
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5186_length_13820_cov_6.986778"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..865)
                     /locus_tag="PKI64_06965"
     CDS             complement(95..865)
                     /locus_tag="PKI64_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOE00784.1"
                     /translation="MKLKYMINQFCKRRVYSAITRRGYSLFPDHCSLIFEYYVGLLVK
                     KGNFTFVQIGANDGIMFDDVRNFIARHAPNASGLLVEPLPDVFVSLQKNYAPFPNVKA
                     VNVAIHNKAKEMTLFRVCDAVAQTDRYRWMSGIASFSKQHLLSHGVRLEDIVEVVVPA
                     MTYSELIAQHMPGPVGLLQIDTEGYDAEIVGQLDLGHENSPSIIRFEHNMIKEAGLSW
                     AQMQDIISRLRQHGYEIAFEEADVLAYRPFALFEFVQA"
     gene            complement(862..2007)
                     /locus_tag="PKI64_06970"
     CDS             complement(862..2007)
                     /locus_tag="PKI64_06970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00785.1"
                     /translation="MKTPRVAHLVYDLIRGGTEGQCARVAMGLSQWGAPQRVAVFHRR
                     GYFLDAVETACGPVHEVAIRHLFRPSTLREIRRLAIWLRQIKADILHTWDADAAIFGQ
                     YAARWAGVKLITSRRDLGQIYPRWKNVMLRWADQRAVRVVANAEAIKKRFMAQGLTAD
                     KMVVLPNMLDLEEFDALAQRPFSRASELPEGRRLVIVNRLDPEKNTGLLIKALPLVRN
                     QIPDAVLIIAGDGPEMPQLRRLAAEQGVQDAVCFLGEVHEVPALLRRCEIGAMVPIRN
                     EGLSNTILEYMAAGLPVLATDCGGNRELVRNRETGIMLPVSADPPRTADVWIALLRSL
                     VSSRDGYHDNRKKLEHHYGPKRVLAEYSSLYLQTDIGGEEGKAGKYR"
     gene            complement(2004..2975)
                     /locus_tag="PKI64_06975"
     CDS             complement(2004..2975)
                     /locus_tag="PKI64_06975"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00786.1"
                     /translation="MVPLSWVAAPYAEFHPGYVVQRDAYGYCNAPAVDGRGYAVVVLG
                     DSFMLALGTQTVAQVLAEVSGLSVYNHAQQGAGPFMEMRRFLASDRFDPPPRFVVWNL
                     SARELGAPLFQRQPIDYWFSQRSQESRAQNSAVSGISWEYLKPSALHGAWPNTSLISF
                     WSRHFWAQARLLVFRAWPHDVLGAEDPQYGPMLFYRENLRMLPLLVPERNATPIVEVV
                     TRVAQRFRERGITLVVLLVPEKEQIHVVALPAADQKALAHGPELLAAIHGGLISNGVE
                     SVNLLPKFREVTDQGTRLYWRDDTHWNDAGIRLAAEELWRVMEPLGP"
     gene            complement(3240..4640)
                     /locus_tag="PKI64_06980"
     CDS             complement(3240..4640)
                     /locus_tag="PKI64_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOE00787.1"
                     /translation="MLFNSYEFLFALLPLTLVGYFLVFPKPWRHAWLTLCSFVFYGWW
                     DYRYCGLLLLAITIDYFAGGRIAAANDPAKKKRWLLVSICSDMGMLGFFKYFDLGART
                     INAAMSLLGAEHPVIPLLHLVLPVGISFYTFQSMSYSIDIYRGNARPARNFIDFACYV
                     SLFPQLVAGPIVRYHELDNQLRQRTHTWAKAGAGITLFVLGLAKKVLLADGVAPIADW
                     GFAQATIGFAGAWSSLLAYTCQIYFDFSGYSDMAVGLGLMLGFQFPQNFNSPYQSLSI
                     TEFWRRWHISLSSWLRDYLYVTLGGNRKGPVRTYINLFLTMLLGGLWHGANWTFVIWG
                     AYHGVLLALERMNHKRGLGWWLPRPLQMSFTFMLVMVGWLLFRAPSLEVFSRLAKGMS
                     GLNGWGATYSFVDAGALQWGILLFSLILVFAARNTWEIRWQPSRRLALGLTFLFLVCV
                     AVFLVNTSSPFLYFQF"
     gene            complement(4644..5921)
                     /locus_tag="PKI64_06985"
     CDS             complement(4644..5921)
                     /locus_tag="PKI64_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOE00788.1"
                     /translation="MSLWAFRFLLWYICVLLVQPQNRFTFLWPLRIANLSFLIATGLH
                     ILSCLEAQKPLLRLGPATLMAFALLFFATLSQHFGVYQISPAWNPYLDIIVKNSLLLI
                     MVEVMVTSIQRVWAVQITSLFCTLWWIKGGLRLSAMGATYSGDRLMGAAVSMIENPNG
                     FAYMMCVFLPLYLYAYQQARHKWEKWFFLACALAAVWIIFETGSRTGLVTLIAIGVFL
                     VPYYGRKHFRALLLITAVLVVMFPMTGEKNRERFRTIPQSIAAFFGGASKGKEPMTMM
                     TQDEQSAEERSEKNKHTWALIKENLLFGVGINPDSSKYVDRYPMASGQVHCEILMAGR
                     QMGIIGMGLYSGFIILIFIQGWRIRKQAVGWPALRDLGWTFQLQAVAFAVGGSFSPGP
                     WNPVMMILAGLSSAMIRLVHEERVDLLASTTAA"
     gene            6177..8702
                     /locus_tag="PKI64_06990"
     CDS             6177..8702
                     /locus_tag="PKI64_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOE00789.1"
                     /translation="MTEGKAQPLAASAFGRRRYVALLLTGLRHACGWTLRAAFLLFLI
                     TLVFFAYLHLVGLPSYFSDALLNRLAAQGYFLQIERLTLEVDRGLVAKNVRLFLKEDA
                     PRPFLESAELSVALNPIPLLWHQESVPLVSIVEGSLRFQVGQNQPETPHGTHELAVTH
                     ANARLSIADRKITLHEFAADFLDIHFRGRGAFYPAARPQAPATNPLALVQQGMEEAPA
                     WVLNIVEQLNRISFDQPPQADFVFSIAPDQPARQSFSLQVRSASGGQVQGVAFSRLDF
                     DVALNEQQVRVPNIQILTTAGILALSGHFDLADQTTLVNLHNTLPPDTLLRVMPPRIQ
                     AQVAAAVPILQFPLQLDLQLGPTAATNLLQQITGRLSFGRTALRNVPIEALDVAFART
                     GPEITIGPASVQFDTGPHASKLSVPEGRYNLTRRDFQARVRGTINPHLLKPLLTPNMQ
                     AIVDWFEFQEPIQGDVMVGGTVGNPAIYCYGPVQATNFSLRNTPIQSFQTQLNITNEV
                     MHMTRALLARPEGMARGDIHMAFSNQTLRLDTVESTLDPRATADMLGPAVATFLKPFV
                     FNGPLRLRINGLLDYCNFSLNQLQAQVEAQGFGFDRWVADTATFDLAADGMRIHVTNA
                     LATAYGGQAVGSGYFYPVGSDTNWRYEVELTATNVNLTELLEASFAKPVKNMRGTLDG
                     TIRVGGYFGQGTGPQVVGSGQATVRGGLLFQTKLFSGLSAILDKILPDFNLFAQTDAN
                     GHFTIRNSRITSRDIDLQGTMFSVKATGDYFLDGNLNYRVEVQLLRNGTLATIVRLAT
                     LPVTRLLEFRLTGTFEDPRWRPVNLNLPDLFSGSHKTAEATSP"
     gene            complement(8728..9933)
                     /gene="hydF"
                     /locus_tag="PKI64_06995"
     CDS             complement(8728..9933)
                     /gene="hydF"
                     /locus_tag="PKI64_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOE00790.1"
                     /translation="MSLSGTPRGERWHIAIFGRRNVGKSSLINALTGQDVAIVSPVKG
                     TTTDPVAKAMELPPLGPVVILDTPGLDDAGELGAQRVQRAHDVLHRADIAIMVTEVQF
                     GFGEHEQALAVQARAKGIPLLLVINKADTAAPTLAARQSMATQARAPLVVVSAVTGEG
                     MDELKLALIRILPEEESPRRLVGDLLRPADVVVLVVPLDKAAPKGRLILPQQQTIRDI
                     LEAGAQALVTRDTELPQTLARLNGRPRLVITDSQVFSQVAAVVPRDVWLTSFSILFAR
                     YKGDLTALVQGARAIDSLPDNARVLVAEGCTHQRQCDDIGTVKIPARLRQYTGKQLVF
                     EHSAGMTYPRELRDYALVVHCGGCMLNRREMQWRLAQAREAGVPIVNYGVLLAHVSGI
                     LPRCLEPFQ"
     gene            complement(9930..10649)
                     /locus_tag="PKI64_07000"
     CDS             complement(9930..10649)
                     /locus_tag="PKI64_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE00791.1"
                     /translation="MMGSLQGKVVLVTGGGRRVGAAICRELAARSAAVVVHYRHSEAE
                     ALALCRELKNGVLAVRGDLSQPAQREWVFREAMEWLGRVDVLVNNAALFARDDEADPA
                     QLAAVNVAAPLDLAARIAAQSGGGCVIHLLDSWTARPEAAAFGEYAATKRELAGSIAR
                     LAQQWVPHTRVNGVAPGPVLVPERTEAAGTVPLQQRPTPEHVAHAVAFLAENTAVTGQ
                     IVYVDGGQHLLAAAHDGRQRP"
     gene            complement(10671..11999)
                     /locus_tag="PKI64_07005"
     CDS             complement(10671..11999)
                     /locus_tag="PKI64_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE00792.1"
                     /translation="MNVTTQPPNHLWNPQCETMPRSELAALQLKRLKKVVSTAWNKVP
                     YYREKMRAAGIRPRDIQTLADVAHLPFTTKDDLRACFPYQAFAVPMENVVRIHASSGT
                     TGQATVVGYTRRDMATWSELMARLQTAAGVTRGDIVQIAFGYGFFTGGLGHHYGVETV
                     GAAVLPVSSGKSERQIQIMKDYGTTVLICTPSYAMHLAEWMAKTPEKERIRLRLGLFG
                     GEAWTEGMRQALQKGLGVFATDNYGLSEVMGPGVSHECVEQKGMHIAEDHFLPELVDS
                     ATGQPVTDPAAGGELVITTLTKEAIPVVRYRTRDLCHFLPGECVCGRTGRRMSKVSAR
                     SDDMLIIRGVNVYPQQIEQALLAIEGVAPHYQIVVSREGTLDELEVLVEVTESIFFDE
                     MTKQQAFKEKIERRLATELAVHARVRLVEPGSTPRSEGKAMRVVDRRKSP"
     gene            12129..13253
                     /locus_tag="PKI64_07010"
     CDS             12129..13253
                     /locus_tag="PKI64_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOE00793.1"
                     /translation="MNSHDDTVWVPCTTLERFMRDVFCQLGTPRAEAAICANVLMAAD
                     EFGFDSHGISRLKPIYYDRIKAGTQLAKTKIRVVKNLRAAAVLDGGNGMGHVVAHRAM
                     TLAIQKARQYGLGMVAVRNSTHFGIAGYYPLMAVKAGMIGICGTNARPSIAPTFGVEN
                     MLGTNPLTFAMPTNDPFPFLLDCATSIIQRGKVEIHAKLNKPLPPGWVIAHDGSSQTD
                     PHAVLADLIAGTAALLPLGGAGEDFAGYKGYGYATVVEILSAALQQGAFLHMLSGRDA
                     RGCATPIPLGHFFIAINIRHFTPLASFKKIAGAILRQLRASRKAPGQKRIYTAGEKEY
                     ATRRERRRRGIPVNLETQREILAMRDELGLRKYHFPFAPN"
     gene            complement(13320..13601)
                     /locus_tag="PKI64_07015"
     CDS             complement(13320..13601)
                     /locus_tag="PKI64_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE00794.1"
                     /translation="MQIREAIIANEQGIHCRPSALIVKAFQGYPGKIRISNGNGSCDV
                     SSVMQLLSLEMHQNCPIKIEVMGENEAAVADRLVEMFQTHFDFPPAAEA"
BASE COUNT         3161 a         4422 c         3485 g         2752 t
ORIGIN      
        1 ccggtgtttg tcgatatcga tccggaaaca tttaatatca accccgctca aatatgcgac
       61 ccaaagcgaa ccatctcaac atggctttct ccaactaggc ttgcacaaac tcaaacaagg
      121 cgaacgggcg gtaggccagc acatccgcct cctcgaaggc gatttcataa ccatgctggc
      181 gcagccgcga tatgatatcc tgcatttgag cccaggacag ccccgcctct ttgatcatgt
      241 tgtgttcgaa ccggataata cttggagaat tctcatgccc gaggtctagt tgaccaacaa
      301 tttcggcgtc atagccttca gtgtcaattt gaagcaatcc cactggcccc ggcatatgtt
      361 gcgcaatgag ttcggaatag gtcatggccg gaaccaccac ttcaacaata tcttccagcc
      421 gcacgccatg actcagcaaa tgctgttttg aaaatgatgc aatgccgctc atccaccggt
      481 atcggtctgt ttgggcaact gcatcgcaca cacgaaataa tgtcatttcc ttggccttgt
      541 tgtgaattgc cacattgacc gccttcacat tgggaaacgg agcataattc ttttgcaacg
      601 agacaaatac atccggtagc ggctcaacca gcagccctga ggcattgggt gcatgccggg
      661 cgataaagtt tcgaacatca tcaaacatga ttccatcgtt ggcaccgatt tgaacaaaag
      721 taaaattgcc cttcttaact aacagcccga catagtactc gaaaatcaaa gaacagtggt
      781 ctgggaacag gctgtatccc cggcgtgtaa tcgcgctata aacccgccgc ttgcagaact
      841 ggtttatcat atatttaagt ttcatctgta ttttcccgct tttccttcct cgccgccaat
      901 atccgtttgt aaatataaac tagaatattc cgcaagaacc cttttgggcc catagtgatg
      961 ttctaacttc tttcgattgt catgatagcc gtctcttgaa ctgacaagcg atcgcaacag
     1021 tgcaatccat acatctgcag tgcgtggtgg atcagccgat acaggcagca ttatccccgt
     1081 ctcccgattc cgcaccaact cgcggttgcc accgcagtcg gtggcgagga ccggcaaacc
     1141 ggcggccatg tattcgagaa ttgtattaga taggccctcg ttgcgaattg ggaccatcgc
     1201 gccgatttca caccggcgca ataacgccgg cacttcgtga acctcgccga gaaagcagac
     1261 cgcatcctgt actccctgct ctgctgccaa gcggcgtaac tggggcattt cggggccatc
     1321 gccggcaatg atgagaacag catcgggaat ctggttgcgc acaagcggca acgccttgat
     1381 caataaaccc gtattctttt ccgggtccaa gcggttcacg atcacaagac gccgcccttc
     1441 tggcagttcg cttgcacgcg agaacgggcg ctgagccaac gcatcaaatt cttccaaatc
     1501 caacatgtta ggcagcacaa ccattttatc tgctgtcaac ccttgggcca taaagcgttt
     1561 tttgatggcc tcggcattgg ccaccacgcg caccgcccgc tgatcggccc agcgcagcat
     1621 gacgttcttc caccgcgggt agatttgccc caaatcacgt cggctggtaa tcagttttac
     1681 ccccgcccac cgggcagcgt attggccaaa aatcgcggca tcggcatccc aggtatgaag
     1741 aatgtccgct ttaatttgcc gaagccagat ggccagccgc cggatttcgc gcaatgtgga
     1801 cggccggaat agatggcgaa tggctacttc gtgaacaggt ccgcatgctg tttcgactgc
     1861 atccaagaaa tatccccgac gatgaaatac ggcgactcgc tggggggctc cccattggga
     1921 caaccccatg gcaacacggg cacattggcc ttccgtgccg cctctaatga ggtcgtaaac
     1981 aagatgggcc acgcgcggag ttttcatggc ccaagcggct ccatcacccg ccacagttct
     2041 tccgcggcca accggatacc ggcatcgttc caatgggtgt catcccgcca atacaaacga
     2101 gtgccttggt ccgtaacctc gcggaacttg ggcagcaaat tgactgattc caccccgttg
     2161 ctgattaacc cgccgtgaat ggccgccaaa agttctggcc cgtgggccaa ggctttttga
     2221 tcagcagccg gcaatgcgac cacatggatt tgctcctttt ccgggaccaa caaaaccacc
     2281 agcgtaattc cacgctcccg gaagcgctgg gcaacccgcg ttacaacctc cacaatgggc
     2341 gttgcgttac gctcgggcac aaggagaggt agcattcgta gattttcgcg ataaaacagc
     2401 atcgggccat actgggggtc ttctgcgccc agcacatcgt gcggccaggc acggaacacc
     2461 aaaagccttg cctgtgccca aaaatggcga ctccagaaag atatcagcga ggtgttcggc
     2521 cacgccccgt gtaatgctga tggcttcaaa tattcccacg agatgccgga aacagcgctg
     2581 ttttgtgccc ggctctcctg tgagcgttga ctgaaccaat aatctatcgg ttgtcgttgg
     2641 aataaaggcg cgcccaactc acgggcggaa agattccaca ctacaaagcg cggcggcggg
     2701 tcgaagcggt cggaggcaag aaaccgccgc atttccatga aaggcccggc cccctgctgg
     2761 gcgtggttat acacgctgag gccactaacc tccgctaaaa cctgcgccac cgtctgagtg
     2821 cccagcgcca gcataaagct atcgcccaaa accacaaccg cgtagccgcg cccatcaacc
     2881 gcaggcgcgt tgcaataacc gtaggcatcc cgctgcacca cgtaaccggg atgaaactcc
     2941 gcgtaggggg cagcaaccca tgaaagtggc accattttgg tcaggcccgc tcccacttgg
     3001 tcaggcacat ggtggactgt cggcttgctt gctgcggcag ggcctgattg cggcaaggtc
     3061 accggcgccg gccaccactg atcctgccaa cggaacacct tccaacgcgc cacccaatct
     3121 ccggcgaaaa gcaagaccca cacgcacaag gcaaacatca tccaccggcg ttgtcgcggt
     3181 tgcggcgctg cggcaaagtc attcaaaact tctggataat ccatgcgcat gaatgggcct
     3241 caaaattgaa aatacaaaaa aggtgagctg gtattgacca aaaatacggc cacgcaaacc
     3301 aagaaaagaa atgttagacc caaggccagc cggcggctgg gttgccagcg tatttcccac
     3361 gtattgcgag ccgcgaacac taaaatcagg ctaaagagta ggatacccca ttgcagtgca
     3421 ccggcatcca caaaagaata agtagccccc cagccgttaa gccccgacat ccccttggcc
     3481 aagcgactaa acacttccaa cgagggagcg cggaacaaaa gccaccccac catgaccagc
     3541 ataaaagtaa aactcatctg gagcggtcgc ggcaaccacc atcccaagcc ccgtttatga
     3601 ttcatgcgtt caagtgccag cagcaccccg tggtaggcgc cccaaataac gaatgtccaa
     3661 ttcgcaccat gccacaaccc gccaagcagc atggtgagaa acagattgat ataggtccgg
     3721 acggggcctt tccggttgcc gccaagagtc acatacaagt aatcgcgcag ccacgaagaa
     3781 agagaaatgt gccagcgccg ccaaaattcg gtgatggata gtgactgata gggcgaattg
     3841 aaattttgcg ggaactgaaa acccaacatg aggcccagtc cgaccgccat gtcggagtag
     3901 ccactaaaat caaaataaat ttggcaggta taagcaagca agctgctcca tgcaccggca
     3961 aagccaatgg tggcctgagc aaatccccaa tcggcaattg gcgccacccc atcggccaac
     4021 aacacttttt tggccagtcc caacacaaac aacgtgatgc cggccccggc tttggcccaa
     4081 gtgtgggtgc gctgacgcaa ctggttgtcc aattcatgat agcgcacaat cggccccgcc
     4141 accaattgcg ggaacagcga aacataacag gcgaaatcaa tgaaattgcg agcgggccgg
     4201 gcgttgcccc gatagatgtc aatgctgtag ctcattgact gaaaggtgta gaatgaaatg
     4261 cccaccggca gtaccagatg cagcagcggg ataaccgggt gttcagcgcc cagcaggctc
     4321 atggcggcgt tgatggtgcg cgcacccaaa tcaaagtatt tgaagaaccc caacatcccc
     4381 atgtcgctgc atatcgaaac caacagccaa cgctttttct tggcggggtc attggcggcg
     4441 gcaatccgtc ccccagcaaa ataatcaatg gtgatggcca gcaacaggag tccgcaatag
     4501 cggtaatccc accaaccata gaagacaaaa ctgcacaacg ttagccaagc gtggcgccac
     4561 ggcttgggga acaccaggaa atagcccacc aatgttagtg gcaagagcgc aaataaaaat
     4621 tcgtaggaat tgaacagcat acgtcaggcg gccgtcgtgg aagccagcaa gtcaacgcgc
     4681 tcttcgtgga ccagccgaat catggcggag gataggccgg caagtatcat cattacggga
     4741 ttccacggcc cgggtgaaaa actcccgccg actgcaaaag caaccgcttg cagttgaaaa
     4801 gtccagccca aatcgcgcag tgccggccag ccgacagcct gcttgcggat tcgccaacct
     4861 tgaataaaga ttaaaataat aaatcccgag taaagtccca taccgataat tcccatctgc
     4921 cggccagcca tcaggatttc gcaatgcact tgcccgctag ccatggggta tctatccaca
     4981 tattttgaag aatcggggtt aattcccaca ccaaacagca ggttttcttt tatgagtgcc
     5041 cacgtgtgct tgtttttttc gctgcgttct tcagcgctct gctcatcttg ggtcatcatc
     5101 gtcatgggct ctttcccttt gctcgcaccg ccaaaaaatg cggcaatgga ctggggaatt
     5161 gtccgaaaac gctcgcggtt cttctcgccg gtcattggga acatgaccac cagcacggcg
     5221 gttatgagca atagcgcccg gaaatgtttc cgtccgtaat aaggcaccaa aaaaacccca
     5281 atggcaatca aggtgaccaa tcccgtgcgg ctgcctgttt caaaaataat ccacacggcg
     5341 gctaacgcac aggccaagaa aaaccatttc tcccatttgt gccgcgcctg ctggtatgca
     5401 tacaaataaa gcggcaaaaa cacgcacatc atataggcga atccattggg attctcgatc
     5461 atggaaactg ctgcccccat caaacgatcc ccggaatagg tcgcgcccat ggcggacagc
     5521 cgtaagccgc ctttgatcca ccaaagcgtg caaaacaatg acgtgatttg caccgcccag
     5581 acccgctgga tcgaagtaac catcacttcg accatgatga gcagcaaaga attctttacg
     5641 ataatgtcga gatatggatt ccaggcggga gaaatctggt aaaccccaaa atgttgcgac
     5701 aaggtggcaa aaaagagcag ggcaaaggcc atcagtgtcg cggggccaag ccgcaacaaa
     5761 ggtttttggg cttctaggca ggaaaggatg tgcagtccgg tggcaatcaa aaatgacaaa
     5821 ttggcaatgc gcagcggcca cagaaatgtg aagcggtttt gtggttgaac caacaacacg
     5881 cagatatacc agagcagaaa acgaaaggcc caaaggctca tggcccgctt gctcccgttg
     5941 ccatggccag ccaaacggac aacatatttt ctctaactgg catctgccga tggttcaagt
     6001 tgacacctaa aaaaatgttc acaattgctt ccgccgagcc acagcagcaa tgcaagtcgc
     6061 ctggcacgag gttcgcccgc cgttgtatct caagaacacg tgagtttcca gtctagagat
     6121 gccgcctcgg cgattcgggc ttttccggtg ccccgcggtg gcgtaggatg ccccacatga
     6181 ctgaaggaaa agcccaaccc ttggccgctt ctgctttcgg caggcggcgg tacgtggcat
     6241 tgctattgac tgggctgcgg catgcatgcg gttggacact gcgggcggct tttttgctgt
     6301 ttctcatcac gctggtgttt tttgcttatc tgcatctggt cggtcttcct tcctattttt
     6361 ccgatgccct actcaaccgc ttggcggccc aaggctattt tctccaaatt gagcggctaa
     6421 ccttggaagt ggaccgcggc ttggtggcta aaaatgtgcg tttattcctc aaagaagacg
     6481 cgccccggcc attcttggaa tccgccgaac tgtcagtggc gctcaatccc atccccctgc
     6541 tctggcatca ggaatccgtt ccgttggttt ccattgtgga aggttccttg cgttttcagg
     6601 tcgggcaaaa tcagccagag acgcctcatg gcacccacga gttggcagta actcatgcaa
     6661 acgcccgcct aagcatcgcc gaccgcaaga tcaccctcca cgagttcgcc gccgattttc
     6721 tggacattca ttttcgcggg cgcggcgctt tttatccggc ggcccgccca caggccccgg
     6781 ccactaatcc attggccctc gttcagcaag gcatggagga agcccccgcc tgggtgctaa
     6841 atatcgtgga gcaactcaac cgaatatcgt ttgatcaacc cccgcaagcg gattttgttt
     6901 tctctattgc gccggatcaa cccgcgcgcc agtctttttc gctgcaagta cggagcgcct
     6961 cgggagggca agtgcagggt gtcgcattca gccgccttga ttttgatgtg gcgctgaacg
     7021 agcaacaggt ccgggtaccg aatatccaaa ttctaaccac cgctggcatc cttgccctct
     7081 ccggccattt tgacctcgcc gaccaaacca ccctggtcaa tttgcacaac acgttgccgc
     7141 cggatacgtt gctgcgcgtc atgccgcctc gcattcaggc gcaagtggca gcggcggtgc
     7201 ccatcctgca atttcctttg caactggacc tgcaactggg gcccactgcc gccaccaatc
     7261 tgctgcagca gatcaccggg cgtctcagtt ttggccgcac cgcactgcgg aatgtgccca
     7321 tcgaagcgtt ggacgtggcc tttgcacgca cggggccgga aatcaccatt ggacccgcaa
     7381 gcgtgcaatt cgacaccggc ccccacgcga gcaaactgtc cgtaccagag gggcgctata
     7441 acttgacccg ccgcgatttt caggcgcggg tacggggcac catcaacccc catctgctca
     7501 agccgctcct gacccccaac atgcaggcca ttgtggattg gttcgagttt caggagccga
     7561 ttcagggcga cgttatggtc ggcggcaccg ttggcaaccc cgcgatttat tgttacggcc
     7621 cggtacaggc caccaacttc tctttgcgca acaccccaat tcaatctttc caaacccaac
     7681 tcaacatcac aaacgaagta atgcacatga cccgggcgct tctcgcccgt cccgagggca
     7741 tggcgcgcgg cgacatccac atggcttttt ccaaccaaac gctgcggctg gacactgtcg
     7801 aaagcacact ggaccctcgc gccacggccg acatgctcgg cccggcggtt gccaccttcc
     7861 tcaaaccgtt tgttttcaac gggccgctgc gcctgcgaat caacggcttg ctggattatt
     7921 gcaacttttc actcaaccag ttgcaggctc aggtggaagc gcaaggcttc ggctttgacc
     7981 gttgggttgc cgacacggct acctttgatt tggccgccga cgggatgcgg atacacgtca
     8041 caaacgcgct ggccactgcc tatggcgggc aggctgtagg cagcggctat ttttacccgg
     8101 tgggctcgga caccaattgg cgttacgaag tggaattgac tgccaccaac gtgaacctga
     8161 cggaacttct cgaagcatcc tttgccaagc ccgtcaaaaa catgcgggga acgctggacg
     8221 gcacgattcg cgtcggcggc tattttggcc aaggaaccgg gccccaagtg gtcggttccg
     8281 gacaggccac ggtccgcggc ggcctgctct ttcagaccaa attattcagc ggtctgtccg
     8341 ctattttgga caagattttg ccggacttca acttgtttgc ccaaaccgat gccaacggtc
     8401 atttcaccat ccgcaacagc cgcatcactt ctcgcgatat tgatctgcaa ggcaccatgt
     8461 ttagcgtgaa ggccaccggt gattacttct tagacggcaa tttgaattac cgcgtagaag
     8521 tgcaactgct ccgaaatgga acgctggcca ccatcgttcg gttggccacc ctgccggtca
     8581 cgcgcctgct ggaatttcgc ctgacgggga cctttgagga cccgcgatgg cgaccggtca
     8641 atctgaattt gccggacttg ttttccggca gccacaagac agccgaggcg acatccccat
     8701 agccgagccg aaattgcccg cgccggctta ttgaaatggt tcgaggcacc gcggcagaat
     8761 gccgctcacg tgcgccaaga gcaccccgta gtttacaatc ggtacgcccg cctcgcgcgc
     8821 ctgcgccaag cgccattgca tctcacggcg gtttagcatg cagcccccac aatgcaccac
     8881 caaggcatag tcccgcagct cgcgcggata cgtcatcccc gccgaatgct caaacaccaa
     8941 ctgtttgccc gtgtactgcc gcagccgcgc gggaattttc accgtgccga tatcatcgca
     9001 ctgccgttga tgcgtgcatc cctccgccac cagcacccgc gcgttgtcgg gcaaggaatc
     9061 gatcgcgcgg gcaccctgta ccagcgcggt caaatccccc ttatagcggg caaataaaat
     9121 ggaaaacgag gtcaaccaca catcgcgggg gaccaccgca gccacttgcg aaaacacttg
     9181 tgagtcggta atcaccaaac gcggccggcc gtttaaacgc gccagcgttt gcggcagctc
     9241 cgtatcccgg gtcaccagcg cttgcgcccc cgcctccaaa atgtcacgga tggtctgctg
     9301 ctgtggcaaa atcagacggc ccttcggcgc agccttgtcc aacggcacca ccaaaacgac
     9361 cacatccgcc ggccttagca aatcgccaac tagtcgccgg ggggattctt cttccggcag
     9421 aatgcggatc aatgccagct tgagttcatc catcccctcc ccggtcacgg cggaaaccac
     9481 caccagcggc gcccgtgctt gcgtggccat gctttgccgc gcggccagcg tgggcgcggc
     9541 ggtatcggct ttgttgatga caagcaacaa cgggatcccc ttggcccgcg cttgcacggc
     9601 caaggcctgt tcgtgctcgc caaagccaaa ttggacttcg gttaccataa tagcgatgtc
     9661 ggcccggtgg agtacatcgt gcgcgcgctg aacccgctgc gcgccgagct cgccggcatc
     9721 gtccaagccc ggcgtgtcca aaatcaccac cggccccagc ggcggcagtt ccatggcttt
     9781 ggccacggga tcagtagtgg ttcctttcac gggcgacaca atcgccacat cttgcccggt
     9841 cagcgcattg atcaagctgg acttgccgac attccgccgg ccgaaaatgg caatatgcca
     9901 ccgctccccg cgcggcgtgc cgctcaagct catggccgtt gcctcccgtc gtgggcggcg
     9961 gccagtaaat gctggccacc atccacatac acaatctggc ccgtcactgc cgtattttcg
    10021 gccagaaagg ccacagcatg cgccacgtgt tcgggcgttg gacgctgctg caacggcacc
    10081 gtgcccgcag cttccgtacg ctcaggaacc aacaccgggc cgggcgcaac accattcaca
    10141 cgggtatgcg gcacccactg ttgcgctaag cgggcaatcg aacctgccag ctcgcgcttg
    10201 gtggcggcat attcgccaaa agctgcggcc tcggggcgtg ccgtccagct atccagcaaa
    10261 tgaatcacgc agcccccgcc ggactgcgcg gcaatccgcg ccgccaaatc caatggcgcc
    10321 gccacattca cagcggccaa ctgggcaggg tcggcttcgt catcgcgggc aaacaacgcc
    10381 gcattattca ccagaacatc cactcgcccc agccattcca ttgcttcacg aaaaacccat
    10441 tcgcgctggg ccggctgtga caaatcacca cgaactgcca aaaccccatt ttttagttcg
    10501 cggcacaacg ccagagcctc cgcttcagaa tgacgataat gcaccacgac cgcggcagaa
    10561 cgtgccgcca gttctcggca aatggccgcg cccactcggc gccccccgcc ggtgaccaac
    10621 accactttgc cctgcaaact acccatcatc ttgccgcggc ttgctccgcc ctagggtgat
    10681 ttccggcgat ccaccacgcg catggccttg ccctccgagc gcggcgtact accgggctcc
    10741 accaaacgca cccgcgcgtg cacggccaat tccgtggcca gccgccgttc gattttttcc
    10801 ttgaaagcct gttgcttcgt catttcgtcg aagaaaatgc tttcggtcac ttccaccagc
    10861 acttccagtt cgtccagcgt gccttcgcgg ctaaccacaa tttgataatg tggcgccacg
    10921 ccttcaatcg ccagcaacgc ctgctcgatt tgctgcggat acacattcac gccgcgaata
    10981 atcagcatgt cgtcgctgcg cgcggacacc ttgctcatgc gccgccccgt gcggccacag
    11041 acgcattcgc cgggcaaaaa gtggcacagg tcgcgcgtgc ggtagcgcac cacggggatg
    11101 gcttccttgg tcagcgtggt aatcaccagc tcacctccgg cggcgggatc cgtcaccggc
    11161 tggccggtcg ccgaatcaac cagctccggc aaaaagtgat cttcggcaat gtgcattcct
    11221 ttttgttcca cacactcatg agaaactccg ggccccatga cttcgctcaa tccgtagtta
    11281 tccgtggcaa acacgcccag ccctttttgc agagcctgtc gcatcccttc ggtccaagcc
    11341 tcgccgccga acaaccccaa ccgcaggcgg atgcgctctt tctccggcgt tttggccatc
    11401 cactccgcca agtgcatcgc gtacgatggt gtacaaatca gcaccgtcgt cccgtaatcc
    11461 ttcatgattt ggatttgccg ctcgctcttg cccgatgaca caggcaacac cgccgccccc
    11521 accgtttcca cgccgtaatg atgccccagc ccccccgtga aaaacccata gccaaaggca
    11581 atttggacaa tgtcgccgcg cgtgactccg gccgcagttt gcagacgcgc catcagctcg
    11641 gaccatgtcg ccatgtcgcg ccgcgtatag cccaccacgg tcgcctgccc ggtggtgccg
    11701 gacgaggcat ggatgcgcac cacgttttcc attggcaccg caaacgcctg atacgggaaa
    11761 cacgcccgca aatcatcctt ggtggtgaac ggtaaatgcg ccacatccgc caacgtttgg
    11821 atatcccgcg gccggatgcc cgccgcgcgc attttctccc ggtaatatgg caccttgttc
    11881 cacgccgtgg acaccacctt tttcaggcgc ttgagttgca gtgctgccaa ttccgagcgg
    11941 ggcatcgttt cgcactgggg gttccacagg tggttgggcg gctgggtggt cacattcata
    12001 tcggaggccc actctagccc ctcccccgcc gtccattcaa caccgaatcc cgccacccgc
    12061 accccgcgcg atcccacttg accgccttcc acccggcccc taggctctca tggcaaggag
    12121 tagccaccat gaacagccac gacgacaccg tttgggtacc ctgcaccaca ctggaacgat
    12181 tcatgcgcga tgtcttttgc caactcggca ctccgcgcgc tgaggccgcc atttgcgcca
    12241 acgtcttgat ggccgcggac gaattcggtt tcgactccca cggcatttct cggctcaaac
    12301 ccatttacta cgaccgcatc aaagccggca cccagctcgc caaaaccaaa atacgcgttg
    12361 tcaaaaactt gcgcgccgcc gctgtcttgg atggcggcaa cggcatgggc catgtagtcg
    12421 cccaccgcgc catgacgctg gcaatccaaa aggcccgcca atatggcctg ggcatggtgg
    12481 ccgtgcgcaa ctccacccat ttcggcatcg ccggctatta cccgctcatg gccgtcaaag
    12541 ccggcatgat tggcatttgc ggcaccaatg cccgcccctc cattgccccc acctttggcg
    12601 tggaaaacat gctcggcacc aacccgctaa ccttcgccat gcccaccaac gatcccttcc
    12661 ccttcctcct cgactgcgcc acctccatca ttcagcgcgg caaagtggaa atccatgcca
    12721 aattgaacaa acccctgccc cccggctggg tcatcgccca cgatggcagt tcccaaaccg
    12781 accctcacgc cgtgcttgcc gatctcatcg ccggcaccgc cgccctcctc ccgttgggtg
    12841 gcgcgggcga agatttcgcc ggctacaaag gctacggcta tgccaccgtg gtcgaaatcc
    12901 tctccgccgc cctacaacaa ggagctttcc tgcacatgct cagtggccgc gatgcccgtg
    12961 gatgcgccac ccccatcccg ttgggccact tcttcatcgc catcaacatc cgccatttca
    13021 cccccctcgc ctcattcaaa aaaatcgccg gcgccatcct gcgccagttg cgcgcctccc
    13081 gcaaagcccc cggccaaaaa cggatttata ccgccggcga aaaagaatat gccacccgcc
    13141 gggagcggcg acgccgcggc atccccgtga atttggaaac ccagcgcgaa atcctcgcca
    13201 tgcgcgatga actcggcttg cggaaatacc acttcccctt cgcgccaaac tgagccccaa
    13261 aacgcgataa aaaagcaacc atcggccgca ccgccaccgg ccacacaaaa aagaaaaatt
    13321 caggcctccg ccgccggcgg aaagtcaaaa tgcgtttgga acatttccac cagccggtcg
    13381 gccaccgccg cctcgttctc gcccatcacc tcgatcttga tcgggcaatt ctggtgcatt
    13441 tccagcgata gcaattgcat cacggaactc acgtcgcacg acccatttcc attggagata
    13501 cgaatcttgc ccggataccc ctggaaggcc ttgacaatca aggccgaggg ccggcagtga
    13561 atcccttgtt cgttcgctat gatggcttct ctaatttgca tgattagttc agcaaagccg
    13621 cgcattcagc cataaggccc ccaccctgtc cagtagaaaa tatccctcaa aaagcccctc
    13681 cccgttcaca ccccgcagac caccgacaat tgaaaaactt ctgccccgct tccgcggggt
    13741 ggtgaagctg ttccctttga aaatcaatgg agccggatag cggaagcagt ccgaactcac
    13801 tatgaccccg agtatgccaa
//
LOCUS       DAOPNP010000064        13779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5200_length_13779_cov_8.142961, whole genome shotgun sequence.
ACCESSION   DAOPNP010000064 DAOPNP010000000
VERSION     DAOPNP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5200_length_13779_cov_8.142961"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..548)
                     /locus_tag="PKI64_07020"
     CDS             complement(255..548)
                     /locus_tag="PKI64_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE00795.1"
                     /translation="MKVKWAPLALDRVAEIAAYISEDNPVAAEKWVRDAFARVGQLKQ
                     FPKSGRPIPETPRKDIRELVWGNYRIIYRMDTRQVSILTVRHTKQILPMEELK"
     gene            complement(545..826)
                     /locus_tag="PKI64_07025"
     CDS             complement(545..826)
                     /locus_tag="PKI64_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE00796.1"
                     /translation="MMRLNLDEDIKSLSEFRANVTACIRQIRETRRPMVITHHGKSAA
                     VLLGASEYESLMQKMEILEDIRLAEDQLANGGGIAHEAALKQVLATVRG"
     gene            1152..2051
                     /gene="murB"
                     /locus_tag="PKI64_07030"
     CDS             1152..2051
                     /gene="murB"
                     /locus_tag="PKI64_07030"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOE00797.1"
                     /translation="MSAILASHTTLRLGGPCRELVTAPDAATAVAAVQAWNAAGTPWR
                     VMGGGSNLLVADAGIPEAVLRFVAESPQCAREGPDLIVVTGGTELDALAQFALHEGCA
                     GLGFAAGIPGTVGGGLVGNAGAFGRQLGDVVEKIEVLTCDGLIQQRQATELTFQYRAS
                     SLQQTGEIVLRAWFRCALGDRGALQAEREEILALRRAKHPDWRVLPTAGSFFKNLPPA
                     KPGEHRQAAGRLLEQAGAKSMREGGAYVFPRHANIVIAETGATARDVARLAARMAAAV
                     QALFGLTLEPEVRYWGDIKVSRE"
     gene            complement(2038..3360)
                     /locus_tag="PKI64_07035"
     CDS             complement(2038..3360)
                     /locus_tag="PKI64_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOE00798.1"
                     /translation="MASIFKAYDIRGIYGETLTDDLAFKIGRAIFAFLGCKKVVIGRD
                     MRPHSKPLFDALARGLVMHGAEVIDLGLCSTPMSYYANKLLGADASIMITASHNPGPW
                     NGFKICRANATPLSGATGIMDLERIVKEEDFCPPAAAKGRITAYDILPAYTEHFRAFA
                     DIRRPLNVLVDYANAMGILEGKTFAGLFKETPLFDTLDGTFPNHEANPLDTHTLVAAQ
                     QAVRAGQYDFAACFDGDADRVGFLDEKGDIIPMDMITALIAKMVLAKNPGANIFYDLR
                     SSWAVKEVILENGGIPHRSRVGHAFIKAQMREKDALFAGELSGHYYFKENSFAESSAL
                     AVLFIANLVSQSDKPLSELIAPIRRYFASGEINSTVSDSARVFQALRAKYADADLLEL
                     DGLSFEYPTWWFNVRSSNTEPLVRLNLEAKTKAEMEQRRDEVLALIRG"
     gene            complement(3439..4116)
                     /locus_tag="PKI64_07040"
     CDS             complement(3439..4116)
                     /locus_tag="PKI64_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE00799.1"
                     /translation="MQMTVKDAANALNVAEKTVYRWIQTGGLPAYRVAGQYRINRAML
                     FEWATSKRINFNAQESLKEPDNLPLPTLATALQDGGIHYRVSGATKNDVLKALVGLLK
                     LPDHVNRDFLFQALLAREQLQSTGVGDGVALPHVRNPAVLEIATPMVCLCFLETPVEF
                     GALDGRPVKVLFTPLSPNVRTHLHLLSRISFALRDAHFKSLLTAEGRRNEIIEAAQAL
                     EPDTPTT"
     gene            4252..5394
                     /locus_tag="PKI64_07045"
     CDS             4252..5394
                     /locus_tag="PKI64_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE00800.1"
                     /translation="MPFIQPMPDLREKLSILAADAQYDLSCACGSTPTDRRRRGLDDR
                     WLYPVTLQDGGHSLMFKTLVSNICSSDCKYCPLRAGQDRVRRCSLTPEEIVRAFMPYW
                     RAGEVLGIFLTSGVCGQPDAAMSRMLATAEILRYRERFRGVLHIKIIPGASDAAIERA
                     LSLASAVSLNLETAGATHFAKLTTRKDYLQNIIAPLKRIAALTAPGAPYARVKTTTQF
                     IVGAADETDREVVNYMEGLYDRLGLHGIYFSAYQRGLGTPDLPGETSAVSNADLLRRE
                     HRLYQTDFLLRKYGFLAAEIGFDDSGNLPLDVDPKEHWARRHRERFPVDVNRADKAEL
                     LRVPGLGPVSVRRILNARRNGGRVRSMESIGLRGARARRVAAWLTY"
     gene            complement(5568..7307)
                     /locus_tag="PKI64_07050"
     CDS             complement(5568..7307)
                     /locus_tag="PKI64_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00801.1"
                     /translation="MNQRPIPRVPAERLEEVSSAYTFSDMEIFVFPSLMYPLVLANLM
                     SPLIWKWREDPWFANFPKLTPYRRILRLKQYIMDHYVFNLDLETWGLTTQQRELDRFA
                     PFLSPETIAKSNALFGYEGDKFYFDLDIRRHFGLEKYTADTIPYWKTETVEAMDAFRY
                     KPGHENQGAGECVSLSTLYAAALYILCGISLDDIFLVATPLHSQNFVDVNEGILTNNR
                     RLVTKTMWFNGTELSTKARRALENEQVTIVAHHTGWVHTVYPEASIDHAAYTRMQTKL
                     RAFLKTPVTSEILFNFLRQSPERQKCFQIEHTIHGKRRWLPAERAYAFEDSCSFKVSD
                     STRSKLLAEMEEDDFFAEPMPNRIPLNKFDEFFKQGQIDLNKEEDRQRLAREVDCYHA
                     NACEIIKELHAFCQLEPRWPDAHKPRQFSRNPELGLKPGMTREEIIATLESIRDTHPV
                     ADLAFHAYRDLSRVDPRPYLKAAIERSPVCIAESRPMDVPMAVACLREMANESIYDST
                     RVAQPDEVWNARRGDGLEKAVTLAAILHERHPEEVFTIQAAGDTATLSFADKEYSFPT
                     HKNLNLTLHWPLD"
     gene            7460..8941
                     /locus_tag="PKI64_07055"
     CDS             7460..8941
                     /locus_tag="PKI64_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOE00802.1"
                     /translation="MLQFSDKVKAKLAALPDQPGVYLMRNRQGRIIYVGKAASLRNRV
                     SSYFRRHTSAKGEPKLRGLIRSIDDFDIIPCKTEAEATLTESRLIKDYRPRYNVSLKD
                     DKRFLLLRVNLHDPFPRFDAVRLRKEDGARYWGPYASSAAARASLEFLDKQFGLRRCR
                     PREPGPEDHRHCLNDIVRFCSAPCIGKVTPEQYRERVLTACAFLDGERREYLENVRQQ
                     MAQAAKELKFEKAAALRDMYLLLLRGIREKARGRKSPQLKKEEARQGLVELQTRLGLP
                     QPPAVIECYDISNISGTNSVASRVVAVEGVPFPARYRMYRIKTVAGADDPASMAEIIQ
                     RRFSRALAEGEALPDLVLVDGGITQLRAARAELDALGLPHQRVAGIAKQFEELYHDPA
                     NEQAPLRFAPDSPALRVLQQIRDEAHRFALTYHRKLRAQRIRESVLDDIEGVGEKRKE
                     QLLAYFGSVDRLRRATEAELAAAPNIGPKFAALIYSVLHPNGN"
     gene            complement(8962..10377)
                     /locus_tag="PKI64_07060"
     CDS             complement(8962..10377)
                     /locus_tag="PKI64_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOE00803.1"
                     /translation="MNASSVAEQIRARMATDGAPPLVMEAFLQNVQRWNAGDVGTIPG
                     ASLAPLGRLPRLDELDEFNPAGESAIAQTVIIKLNGGLGTSMGLEQAKSLIIAKNGLS
                     FLDIIVRQVEFLRQRCRAPLPLLLMNSFSTETDSLAALAQTHPRFHNPAGLPLSFVQH
                     RVPKLSLATRAPVSWPTQPELEWCPPGHADIYYALQTTGLLDKLLAGGFRYAFVSNAD
                     NLGALLHLGILGHIASHNVPFLMEVTARTECDRKGGHLGRDAATGRLLLRESAQCPPD
                     ETEDFQDITKFSLFNTNNLWLDLAALREMLRAADGLPQLPLIVNRKTVDPVRSDSPAV
                     VQLEAAMGSAISCFDNAAALVVPRFRFAPVKTTNDLLAIRSDLYTLGEEHQVALHPQR
                     FSPPPAITLDARYYRNIADFEQRFAKGAPSLIACDSLDITGDIYFGANVRIEGAVHLR
                     NPTDTPARIADGTRLHTPIRP"
     gene            complement(10392..11273)
                     /locus_tag="PKI64_07065"
     CDS             complement(10392..11273)
                     /locus_tag="PKI64_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00804.1"
                     /translation="MALINFDCPECGHNLEVDERGAGFIIKCPECANPLQIPELPKAR
                     RIRKITMAAITLVALVVLCLNNIYLWQRGNRLRQEVANLQPLQVALQQAQEISMQQET
                     EISRLQGQLKSIKVPDMTAWHEAAQAAVNEAELLARELEDTSRRLLDSSADERTALLR
                     RYMAKEIAAAKDGLPAQPIIKDVNPGQGINGRQIIFPILPGPEGQVLRENAEIIAVDG
                     DKVSVKHSGGVHTYSLPELHRGVAAFLPVDPLLVLPRNQRNATILHVHQTQNAIRDQK
                     IKQLRDTLDDLLAATEP"
     gene            complement(11408..12088)
                     /locus_tag="PKI64_07070"
     CDS             complement(11408..12088)
                     /locus_tag="PKI64_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOE00805.1"
                     /translation="MSEYPIIKRIAARLPARWQTELKRIRCARQIRKGTFATNEPEYD
                     RLAEWLRPGDWVLDIGANVGHYTKRFSELVGAQGRVIAFEPTPTTFSLLASNSQHFAH
                     PNVTLINAAVSDRADLVGLAIPQFDSGLVNYYEAHLAPARPGTLAVMTLAIDSLQINH
                     RIALIKIDAEDHEAAVLAGMKNLLARDHPILIVETGAQTVVDALTASGYASHKLPGSP
                     NFLFMPAI"
     gene            12147..13616
                     /locus_tag="PKI64_07075"
     CDS             12147..13616
                     /locus_tag="PKI64_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE00806.1"
                     /translation="MKPEKYTSNLLQSRYLAWCHRELLFMHRLAESLIWMLVTAFRFV
                     AIAVAIMLFVFPAYKGVWAGYLPLLLAAILLRGGEGFYNLIQSGLQSFSEARYYVLLL
                     ILSVGIQFALILIFRPEPASDGFFVYREACRLLETGGMDPLTYYAPAQIWYYALFFKL
                     FGSSFLTAQLCQLPLAALIPLLGYIIARRVTPTRQARWAGLCIGIYPGLNAYILVTPY
                     YFYMYTVAILFVVWAWVRILARADDWISAAWGGLAAGWGALTKATLLVAPVQTLFLWM
                     LASGCTNYRKRWIAWLIFSIMMASVVAPWTIRNKQVFGETVLVNTAGPLVFYSSNNPE
                     SNGLYSSIPDETPVRTSDEMLAHMASCNKRAWAFIREQPVKFTRLVLLKLLHTWGTET
                     SFVEFINWHGAPLGTLDPAMRLVIQIGWATLVLAWCFRATAALWQRLPPSVLEIATSI
                     VVLSKIIIYSIYEGGARHHMPAIPLLIVCVISLTKQRKK"
BASE COUNT         2699 a         3705 c         4315 g         3060 t
ORIGIN      
        1 gcgagcggtt gagtccagaa aattggcgtt ttggcgtcaa aatggcgtgt ttttcgggtt
       61 ttggggtcat tttgaggcgt ttttggcgat tttggggcgc aaaaccggca cttttactca
      121 atttttggcg taacggcacc cttccgcgag ggtgttaagg ttttggggcc cactcctgat
      181 ttattgatcg tcacgggtcg gtggactgtt agtccgctgc tggcggtgcg taggcatgaa
      241 aaacatggtt gggtctattt cagttcttcc ataggtagga tctgctttgt atgccgaacc
      301 gtcaggatcg aaacctggcg ggtatccatg cggtaaatga tgcggtaatt gccccagact
      361 agttcccgga tgtctttacg cggcgtttcc ggaatgggtc tgccgctttt gggaaattgt
      421 ttcaattgtc cgacacgcgc gaaagcatcg cgaacccatt tctcggcggc aaccggattg
      481 tcttccgaaa tataggctgc gatttctgca acccgatcaa gggccagcgg tgcccatttt
      541 actttcatcc gcgcaccgtg gccaacactt gtttgagcgc ggcttcatgc gcaatgccac
      601 cgccgtttgc gagttggtct tcggcaaggc ggatatcctc aagaatctcc atcttttgca
      661 ttaaactctc atattccgag gcacccaaga gtacggcggc ggatttacca tgatgggtga
      721 taaccatcgg acgccgggtc tcccggatct ggcggatgca cgccgtgaca ttcgcgcgga
      781 attcggatag ggacttgata tcttcgtcta aattgaggcg catcattgtt aatctccctt
      841 gtactatcaa acgtacatta ttatgtacgc atgtcaagac ttgctgccaa gctgaaaatg
      901 gcgttttagc atcaaaatgg cgtgtttttc tgattttttg gctattcttg ggcatttatg
      961 acgattttga ctggcaaaac aggcgcttta tcacattttt cgccgtaaca tcacccttca
     1021 gcgagtgagt aggatcttgg gagttacttt ttttgtttta tggcacaaaa gcgggggtaa
     1081 ggagcaagac aacgctcgag aaaagcggca aaaggctggc gcgggggggg gcgtctgcgt
     1141 aggatgaggc aatgagtgca atcttggcca gccataccac gctgcgtttg ggcggtccct
     1201 gccgcgaact ggtgactgcg cccgatgcag cgacagcggt cgcggcggtg caggcatgga
     1261 atgccgccgg cacaccgtgg cgggtgatgg gcgggggctc gaacttgctg gtggcggatg
     1321 ccgggattcc cgaggcggta ctgcgctttg tggccgagtc gccgcaatgc gctcgcgagg
     1381 ggccggacct cattgtggtg actggcggca ccgagctgga tgcgctggcg cagttcgcgt
     1441 tgcatgaagg ttgcgctggg ttgggatttg ccgcaggcat ccccggcacc gtgggtggcg
     1501 gcttggtggg taatgccggg gcatttggcc gacaactggg cgatgtggtc gagaaaatcg
     1561 aagtgctgac ctgcgacggc ctgattcagc agcggcaggc aaccgaactg acttttcagt
     1621 atcgggcgtc atcgctgcaa caaaccggcg agattgtgct gcgggcatgg ttccggtgtg
     1681 cgctgggtga tcgcggggct ttgcaggcgg aacgcgagga gatattggcg ttgcgtcgcg
     1741 ccaagcatcc cgactggcgc gtgctgccga cggccggcag ttttttcaag aatttgccgc
     1801 cggccaagcc gggcgagcac cgacaggcgg cgggccgcct gctggagcaa gccggcgcaa
     1861 aatccatgcg cgaaggcggg gcctacgttt ttccgcggca tgccaatatc gtcattgcgg
     1921 aaacgggggc caccgcgcgg gatgtggcgc ggttggcggc acggatggcg gctgctgtgc
     1981 aagccttgtt tggcctgaca ctggagccgg aagtgcgcta ctggggcgat atcaaggtta
     2041 gccgcgaata agcgcgagaa cttcgtcgcg ccgctgctcc atctcggcct tggtcttggc
     2101 ttcgaggttc agccgcacca gaggctcggt gttggagctg cggacgttga accaccacgt
     2161 gggatactcg aagctcaagc cgtccagttc cagcaagtcc gcatcggcat atttggcgcg
     2221 tagcgcctgg aaaacccgcg cggaatcgct gacggtggaa ttgatttcgc cgctggcaaa
     2281 ataccgccgg atgggggcga tcagctccga gagcggcttg tcggactgcg agacgagatt
     2341 ggcgatgaac agcaccgcca gcgcggaaga ttccgcaaag gagttttcct tgaaataata
     2401 gtggccggaa agttccccgg caaagagcgc atccttttcg cgcatttggg ccttgatgaa
     2461 cgcatggccc acgcggctgc ggtgtggaat gccgccattt tccagaatga cttccttcac
     2521 ggcccacgag gagcgcaggt catagaaaat gttggccccg ggatttttgg ccagcaccat
     2581 cttggcgata agcgcggtga tcatgtccat ggggatgatg tcgccttttt cgtccaaaaa
     2641 cccgacgcgg tcggcatcgc catcgaagca ggcggcaaaa tcatattggc ccgcgcgcac
     2701 ggcctgttgc gcggccacca gggtgtgggt gtcgagcggg ttggcctcat ggttggggaa
     2761 ggtgccgtcg agcgtgtcga acagcggggt ctccttgaac aggccggcaa aggttttgcc
     2821 ttcgaggatg cccatggcgt tggcgtaatc caccagcaca ttcagggggc ggcggatatc
     2881 ggcaaacgct cggaaatgtt cggtgtaggc gggcaaaata tcgtaggccg tgatgcgccc
     2941 cttggcggcg gcgggcgggc agaaatcctc ttctttgacg atgcgttcca gatccatgat
     3001 gccggtggcc cctgaaagcg gcgtggcgtt ggcgcggcaa atcttgaagc cgttccacgg
     3061 ccccggattg tgcgaggccg taatcatgat ggaggcatcg gcgcccagca gtttgttggc
     3121 gtaatacgac atgggcgtgg agcagagccc caaatcaata acctcagccc cgtgcatgac
     3181 cagaccgcgg gccaacgcat cgaacagcgg cttggagtgg gggcgcatgt cgcggccgat
     3241 caccaccttt ttgcagccaa ggaaggcaaa aatcgcgcgg ccgatcttga aggcgaggtc
     3301 gtcggtcagg gtttcgccgt agatgccgcg gatgtcgtat gccttgaaga tgcttgccat
     3361 gacttgctcc tgtgaggggt ttgcttgtgg gtcagtgtaa tgccgggggc gggatgtcgc
     3421 aagggtcttt gcgcgcggtt aggtcgtggg ggtgtccggt tcgagcgctt gggcggcttc
     3481 gatgatttca ttgcggcgac cttcggccgt cagcaggctc ttgaagtggg catcgcgcaa
     3541 agcgaaggaa atccgggaga gcaggtgcag gtgggtccgg acgttggggg agagcggggt
     3601 aaagagtacc ttgaccggac ggccatccag cgcgccaaac tccaccggag tttcgaggaa
     3661 gcacaggcaa accatgggcg tggcgatttc cagcacggcc ggattgcgca cgtgaggcag
     3721 ggccacgccg tcgccgacgc cggtggactg caactgttcg cgtgccagca gcgcctgaaa
     3781 aaggaaatcg cgattgacgt ggtccggcag cttgagcaac cccaccagcg ccttgaggac
     3841 atcgtttttt gtcgccccac ttacgcggta atggatgccg ccgtcttgca gcgcggtggc
     3901 cagcgtgggt agcggcaggt tgtccggctc tttcaggctt tcctgggcgt tgaaattgat
     3961 ccgtttggag gtggcccatt caaacaacat ggcccggttg attcggtatt gccccgccac
     4021 ccgataggcc ggaagaccgc cggtttgtat ccagcgataa accgtttttt ccgccacatt
     4081 cagggcattt gccgcatcct tgactgtcat ctgcatggcc gacctcctga gcgagtattc
     4141 tttcccaacg gtatgcccct tgtcccaaat tgtcaatctt tgagaaaaat gtggcgatgg
     4201 tgagaaagct catttcttga tgcggaacaa tagttcactt atgattgttc aatgcccttc
     4261 atccagccaa tgcccgattt gcgggagaaa ctgagcattc tcgccgccga tgcgcagtac
     4321 gacctgtcct gtgcctgcgg ctcgacgccg acggaccgcc ggcggcgtgg gctcgacgat
     4381 cgctggcttt atccggtgac actacaagac ggtgggcata gcctcatgtt caaaacgctg
     4441 gtgtccaaca tttgctccag cgattgcaaa tactgcccgc tgcgcgcggg gcaagaccgc
     4501 gtgcgccgct gctcgctgac gccggaagaa atcgtgcggg cgttcatgcc ctattggcgc
     4561 gcgggagaag tcctcggcat ttttctcacc agcggggttt gcggtcagcc cgatgccgca
     4621 atgtcgcgta tgttggccac cgcggaaatc ctgcgctacc gcgagcgctt ccgcggggtg
     4681 ctgcacatca aaatcatccc cggcgcctcg gatgccgcca ttgagcgcgc gctatccttg
     4741 gcttccgcgg tatcgcttaa tctggaaacc gccggcgcga cgcatttcgc caaactgacc
     4801 acccgcaagg actacctgca aaatataatc gcgccgctta agcgcattgc cgcgctgact
     4861 gcacccggcg cgccctatgc gcgggtcaaa accaccacgc aatttattgt gggcgctgct
     4921 gacgaaaccg accgcgaagt cgtcaactat atggaaggcc tctacgatcg cctcggattg
     4981 cacggtattt atttcagcgc ctaccaacgc gggctcggca cacccgattt accgggcgaa
     5041 accagcgccg tgagcaacgc ggacttgctg cggcgagagc atcggctgta tcagaccgat
     5101 tttctgctgc gcaagtacgg gtttctggcc gcggaaatcg gctttgatga ttccggcaac
     5161 ctgccgctgg atgtggaccc caaagaacat tgggcgcgtc gccatcggga gcgtttcccg
     5221 gtggacgtga atcgggcgga caaagcggaa ctgttgcggg ttcccggctt aggaccggtg
     5281 agcgtcagac gcatcctgaa tgcccggcgc aatggggggc gagtacgcag tatggaaagt
     5341 atcgggttgc gcggcgcaag ggcgcgacgc gtggcggctt ggctaacgta ttgaatcgcc
     5401 cgggccggtg cagcggcggc gtgggggagg agttcagcat tcggactctc aaccgtggct
     5461 gttcttgtcg cgtttttgta gggcgggaat ggttgaggaa cgaaaccttc ccgccgggaa
     5521 tggctggaag gtctgcgacc attccagttt tacaatccaa cccggcttca gtccagcggc
     5581 cagtgaagcg tcaggttcag attcttgtgc gtggggaagg aatactcctt gtcagcaaag
     5641 gagagggtgg cggtgtcgcc cgcggcctga atcgtgaaca cttcctcagg atggcgctcg
     5701 tgaagaattg cggccaacgt gacggccttt tccaggccgt cgccccggcg ggcattccag
     5761 acctcgtctg gctgcgccac acgggtgctg tcataaatgg actcattggc catttcgcgc
     5821 aggcacgcga ccgccatggg gacatccatt gggcgcgatt ccgcgatgca cacgggagag
     5881 cgttcaatgg cggccttgag gtaggggcgg gggtccacgc gggacaaatc gcgataggcg
     5941 tggaacgcca gatcagcaac agggtgggtg tcgcggatac tttccagcgt ggcaatgatt
     6001 tcctcgcggg tcatgccggg tttcaggccc agctccgggt tccggctgaa ttgccgcggc
     6061 ttgtgggcgt caggccagcg cggctccagt tggcaaaacg cgtgcagttc cttgatgatc
     6121 tcgcaggcgt tggcgtgata gcagtcaacc tcgcgcgcga ggcgctggcg gtcttcctcc
     6181 ttattgaggt caatctggcc ctgcttgaag aattcatcaa acttgttgag cggaatgcgg
     6241 ttgggcatgg gttcagcgaa gaaatcatct tcttccattt ccgccagcag tttgctccgg
     6301 gtgctgtcgg aaaccttgaa ggagcatgag tcctcaaacg cgtaggcgcg ttccgccggc
     6361 aaccagcggc gcttgccgtg gatggtgtgc tcaatttgga agcacttttg gcgctccggg
     6421 ctttgacgca agaaattgaa caagatttcc gacgtgaccg gggtcttaag gaacgcgcgc
     6481 agtttggtct gcatccgggt ataggccgcg tggtcaatgc tggcttcggg atagacggtg
     6541 tgcacccagc cggtgtggtg tgccacgatg gtaacctgct cattctccag cgcgcggcgc
     6601 gccttggtgg acagctccgt gccgttgaac cacatggtct tggtaacgag ccgccggttg
     6661 ttggtcagga tgccttcatt gacatcaaca aagttctggg agtgcagcgg ggtggctacc
     6721 agaaaaatgt catccagcga aattccgcac aggatgtaca gcgccgcggc gtacaaggtg
     6781 gagagcgaga cacattcgcc cgcgccctgg ttttcgtggc cgggcttgta gcggaaagca
     6841 tccatggcct cgaccgtttc cgttttccag tacgggatgg tatccgcggt atatttctca
     6901 aggccgaaat gccggcggat gtccaaatca aaatagaact tgtcgccttc gtagccgaag
     6961 agcgcgttgc ttttggcaat ggtttccggc gagagaaatg gcgcgaaacg atccagctcg
     7021 cgttgctgcg tggtcaggcc ccaggtttcg aggtcgagat tgaacacata atgatccatg
     7081 atgtactgct tcaggcgcag gatgcggcgg tacggagtca gcttggggaa attggcaaac
     7141 cacgggtctt cccgccactt ccagattagc ggcgacatca agttggccag caccagcgga
     7201 tacatcaggc tggggaacac aaagatttcc atgtcggaaa aagtgtacgc ggaagagact
     7261 tcttcgagcc gctccgccgg cacgcggggt atgggacgtt gattcatggc gggcaagata
     7321 cggcccaacc gcgcccactg caagaagtaa ggtcaggctc agcgccaacc atgccccgtg
     7381 gccgggccgg gcgtgttcat ggtgcagttt tctttgccat tgcgtggcaa tgcggcggca
     7441 acctagtatc ttttctgcaa tgctgcaatt ttcggacaaa gtgaaggcca agctggcggc
     7501 gctgcccgat cagccgggcg tgtatttgat gcgcaaccgg caaggccgca ttatttatgt
     7561 gggcaaagcg gccagcctgc gcaatcgggt ctcatcctat ttccgccgcc atacctccgc
     7621 caaaggcgaa cctaaattgc gtgggctgat ccgcagcatt gacgacttcg acatcatccc
     7681 gtgcaaaacg gaggcggagg ccacccttac agaaagccgg ctaatcaagg actaccggcc
     7741 gcgctataat gtgtcgctca aggatgacaa gcgctttctg ctgctgcgcg tgaaccttca
     7801 cgatccattc ccgcgttttg atgctgtacg cctgcgcaaa gaggatggcg ctcgctactg
     7861 gggcccctat gcatcgtccg ccgcggcgcg ggcctcgctg gaatttttag acaaacaatt
     7921 cggtttgcga cgctgccgcc cacgggagcc cggtccggag gatcaccgcc actgcctgaa
     7981 tgacattgtg cgtttttgct ccgccccgtg tatcggcaaa gtcaccccgg agcaataccg
     8041 ggagcgggtg ctgaccgcct gcgcctttct ggatggcgag cggcgcgagt atctcgaaaa
     8101 cgtccgccag caaatggcgc aggccgcgaa agagctgaaa ttcgaaaagg ccgcggcctt
     8161 gcgagacatg tatttactgc tgctgcgcgg tatccgtgaa aaagcacgtg gccgtaaaag
     8221 tccgcaactg aaaaaagaag aagcccgcca aggtctggtg gaactgcaaa cccgtttggg
     8281 gttgccgcag ccgccggccg tgattgaatg ctacgatatt tccaacattt ccggcaccaa
     8341 ctcggtggcc agccgcgtcg tggcggtgga gggcgtgcca ttcccggcgc gctaccggat
     8401 gtatcgcatc aaaaccgtgg cgggcgcgga tgaccccgcc tccatggccg agatcattca
     8461 acgccgcttt tcccgcgcgc tggccgaagg cgaggcgctg ccggatttgg tgctggtgga
     8521 tggcggcatc acgcaactac gcgcggcgcg ggcggaactg gatgcgttgg gattgcccca
     8581 ccagcgggtg gcgggtatcg ccaaacagtt tgaggagttg tatcacgacc cggccaatga
     8641 gcaggcgccg ctgcggtttg cgccagattc gccggcgctg cgcgttttgc agcaaattcg
     8701 ggacgaagcg caccggtttg cgctgaccta tcaccgcaag ttgcgcgccc agcggatccg
     8761 agaatcggtg ttggacgaca tcgaaggcgt gggcgaaaaa cgcaaagaac aactgttggc
     8821 ctattttggt tcggtggacc gactgcggcg agccacggaa gcggagctgg ctgccgcacc
     8881 gaatatcggt cccaaattcg ctgcactgat ctactctgtg ctgcatccca acggtaattg
     8941 actccgcgcg cgggtaggga gctacgggcg gatgggcgta tgcagccggg taccgtccgc
     9001 aatccgcgcc ggggtgtccg tggggttgcg taaatgcacc gcaccttcaa tgcggacatt
     9061 tgcgccaaaa taaatatcgc ccgtaatgtc gagagaatcg caggcgatca gcgatggcgc
     9121 gcctttggca aagcgctgct caaagtcggc aatattgcgg taataccgcg catctagcgt
     9181 gatggccggt ggcggactga agcgctgggg atgtagtgcc acctgatgtt cttcgcccaa
     9241 ggtgtagagg tcggaacgaa tggccagcag atcgttggtg gttttgaccg gtgcaaagcg
     9301 aaagcgggga accaccagcg cggcggcgtt gtcaaagcag ctaatggccg aacccatggc
     9361 ggcttccaac tgcaccacgg ccggggagtc gctccgcacg gggtccaccg ttttgcggtt
     9421 cacaatcaac ggcagttgcg gcaggccgtc ggcggcgcgg agcatttcgc gtaaggcggc
     9481 caaatccagc cagaggttgt tcgtgttgaa cagcgaaaac ttggtgatgt cctgaaaatc
     9541 ctcggtttcg tccggcgggc attgggccga ttcgcgcaac agcaggcggc cagtggcggc
     9601 gtcgcgcccg aggtggccgc ccttgcggtc gcattcggtg cgggcggtca cttccatcag
     9661 gaagggcacg ttgtggctgg cgatatggcc cagaatgccc aggtgcaaca gtgcgcccaa
     9721 gttgtccgcg ttggaaacaa atgcgtagcg gaagccacca gccagtagtt tatcgagcaa
     9781 cccggtcgtt tgcagcgcgt aataaatatc tgcgtgtccc ggcggacacc actccaactc
     9841 gggttgcgtc ggccacgaga ccggcgcgcg cgttgccaat gatagctttg gcacccggtg
     9901 ctgcacaaat gacaatggca aacccgctgg attgtggaag cgcgggtggg tttgggccaa
     9961 ggccgccaac gaatcggtct ccgtcgaaaa gctattcatc aacagcagcg gcaacggcgc
    10021 tcggcagcgc tggcgcagga attccacctg tcggacgatg atatccaaaa atgacagtcc
    10081 gtttttggcg atgatgagtg atttagcctg ctccagcccc atgctggtgc caaggccgcc
    10141 gttaagcttg ataatgaccg tctgcgcgat ggcgctttcg ccggccggat tgaattcatc
    10201 caattcgtct aggcgcggca accgcccgag aggggcgaga ctggcgccgg gaatggtgcc
    10261 cacatcaccc gcgttccagc gctgcacatt ttggaggaat gcttccatga ccaacggcgg
    10321 cgcgccgtcg gtggccatgc gggcacggat ttgttcggca acggatgatg cgttcatgtc
    10381 aacgttggcc ctcacggttc ggtggccgcg agcaggtcat ccaacgtatc gcgcagttgc
    10441 ttgatttttt ggtcgcggat ggcgttttga gtttggtgta catgcaggat cgtggcgttg
    10501 cgctggttgc gcggcagcac cagcaagggg tccactggca ggaaggcggc tacgccacga
    10561 tgcaactccg gcagggagta agtgtggact ccgccggaat gtttcacgga gactttgtcg
    10621 ccatcaactg caatgatttc agcattttcg cgcagcactt ggccttccgg accaggcaaa
    10681 atcggaaaga tgatttgccg accattgatc ccttggcctg ggttgacatc cttgatgatc
    10741 ggttgcgcgg gcaagccatc cttggctgcg gctatttctt tggccatgta gcggcggagc
    10801 aaggcggtgc gttcgtctgc gctgctgtcg agcaggcggc ggctggtgtc ttccaattcg
    10861 cgggcgagca gttcggcctc gttcacggcg gcctgcgcgg cttcatgcca agcggtcatg
    10921 tcgggcactt tgatggactt caactgccct tgcaggcggc tgatctcggt ctcctgctgc
    10981 atgctgattt cctgcgcttg ttgcagggcc acttggagcg gttgcaaatt ggcgacttct
    11041 tggcgcagcc ggttgccgcg ttgccacaaa tagatgttat tgaggcagag gaccaccaac
    11101 gccaccaagg tgatggcggc cattgtgatt ttccggatgc ggcgtgcttt gggcaattcc
    11161 ggaatttgca gcggattggc gcactcgggg cacttgatga tgaaacccgc cccgcgttcg
    11221 tccacttcca gattatgtcc gcattccggg caatcaaaat tgatcaatgc catatacgcc
    11281 tgctttctcg tgggcaaacc ccacgccggc aatataggga tacccgcggc catgtgcaag
    11341 tatgcccttg ttaggcagac aggcgttgcc gagtttgcgc cctaatccgc gcgcggcagc
    11401 caggcggcta gattgccggc ataaataaaa aattgggcga tcccgggagc ttgtgagacg
    11461 catagccaga cgcggtcaag gcatccacaa cggtctgggc gcctgtttcc acaatcagga
    11521 tgggatggtc tctggcgagc agatttttca tgccggccaa gacggcggct tcatgatctt
    11581 ccgcgtcaat tttgatgagc gcgatgcggt gattgatctg caaagaatca atggccaggg
    11641 tcatgactgc taatgtgccg gggcgggccg gcgcaagatg ggcctcatag taattgacga
    11701 gaccggaatc gaactggggg atggccaggc ccacaaggtc ggcgcgatcg gagacggccg
    11761 cgttgattag cgtcacgttg ggatgggcga agtgttggct gttgctggcc agcaaggaga
    11821 atgtggtggg cgtcggttca aaagcgatga cccgcccctg cgcgccgacc agctcggaaa
    11881 atcgtttggt gtaatgaccc acattggcgc cgatgtccaa gacccaatcc ccggggcgga
    11941 gccattcggc caagcgatcg tattccggct cgttggtggc gaatgttccc ttgcggattt
    12001 ggcgggcgca gcggatgcgc ttcagttccg tttgccaacg ggcgggcaaa cgggccgcga
    12061 ttcgtttgat tattgggtat tcactcattg ccacatccta gcgcggaggc gaaaagattc
    12121 caattgtttc ccgcttgcgt tggtaagtga aaccggagaa atatacttca aacttgcttc
    12181 aatcacgtta tcttgcatgg tgtcatcgcg aattgttgtt catgcatcgt ttagcagaga
    12241 gtcttatttg gatgttggtt acggcgttcc ggtttgtcgc catcgctgtg gcaatcatgc
    12301 tttttgtatt tcctgcgtac aagggcgttt gggcgggata tcttccgttg cttctggccg
    12361 cgattctgct ccggggggga gaggggtttt acaatctcat ccaatcagga cttcaatctt
    12421 tttctgaggc gcgttattat gttcttcttc tcattctttc tgttggtatt cagtttgcgc
    12481 tgatcctcat ctttagacct gagccagctt ccgatggctt ttttgtctat cgagaagcct
    12541 gtcgcttgct ggaaacagga ggcatggatc cactgaccta ctatgcaccc gcgcagattt
    12601 ggtactatgc cttgttcttc aagctctttg gttcgtcctt tctgaccgca cagctatgtc
    12661 aattgccttt ggctgcactc attccgttgt taggatatat cattgcgcgt cgcgttacac
    12721 ccactcggca agcacgttgg gcgggattat gcatcggaat atacccggga ctgaacgcct
    12781 atattctcgt cacgccgtac tacttctata tgtacactgt cgcaatcctt ttcgttgttt
    12841 gggcatgggt gcgcattttg gcgcgagccg acgactggat cagcgcggcg tggggcggac
    12901 ttgcagcagg gtggggggca ttgaccaagg ccacactgct tgtggcaccg gtgcaaacac
    12961 tctttttgtg gatgctcgca tcaggatgta ccaactaccg aaagcgatgg atagcttggc
    13021 tgatcttcag tattatgatg gcatcggttg tggccccatg gacaatccgc aataaacaag
    13081 tatttggtga gaccgtgctg gtcaacacag ctggtccatt ggtgttttat agctccaaca
    13141 acccggaatc gaacggtttg tatagttcga ttcccgatga aacgcctgtc cgaacttcag
    13201 acgaaatgct cgcacatatg gcctcttgta ataaacgggc ttgggcgttt atccgggaac
    13261 aacccgtcaa gttcacccgt ttagtcttgt tgaaactcct tcatacatgg ggcactgaga
    13321 cttcctttgt ggaattcatt aattggcatg gcgcaccatt gggtactttg gatccagcca
    13381 tgagacttgt catacaaatc ggctgggcga cgctggtttt ggcatggtgt tttcgcgcca
    13441 ctgccgcatt atggcagcgt ctcccgccat cggtgttgga gattgccacc agtattgtgg
    13501 tcctatcaaa aattattata tactctatat acgaaggggg agcacgccat cacatgcccg
    13561 ccattccgct gttaatcgtc tgtgtcatat cgctgaccaa gcagcgcaag aaataagaag
    13621 aacgttccgc ttgtgttagg gcgttgcgtt aagtggcgat tggtagtgag caggatagag
    13681 aggcgttcat tccgggtgct gacaacttgg aagatcagga cggtgccttg cttatcggtg
    13741 ggcggctagc cgagctcctt gaagatgagc aagtcgggg
//
LOCUS       DAOPNP010000065        13751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5215_length_13751_cov_9.162237, whole genome shotgun sequence.
ACCESSION   DAOPNP010000065 DAOPNP010000000
VERSION     DAOPNP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13751
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_13751_cov_9.162237"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PKI64_07080"
     CDS             complement(<1..570)
                     /locus_tag="PKI64_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00807.1"
                     /translation="MIEITLQVDHKSALQGSGINGFLLNSDGFLALPGFPQGTRVGVE
                     DVRVFLLGQVHRFFDEAGGEGNIALPDAAAGQKGPGDGVQGVGAFQVGGLDLASQFSG
                     LARIFRLPDSFEVLPEKFGNSLGFQGLLFFGCRSFLGPLQLKTTGGNFLKNLPHLAHR
                     MADPRNPALMDYKSRGDAALLGGLHPVFRH"
     gene            898..2238
                     /locus_tag="PKI64_07085"
     CDS             898..2238
                     /locus_tag="PKI64_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOE00808.1"
                     /translation="MPVAVARPGVVVVGQRLYVIGGAQGEGEPRFKTVQIFDIPTETW
                     TLGANLPVAREGHVFEYEGKIYAPGGYDGNLAIRDFQVYDPARNRWRLWSPLPNKMSA
                     HHGCVVDGQLYLFGDYDELDRTVVCDLKTKKWARIAVGYRPARHAALASTGKDIFVLG
                     GNVQSSSPFLARIQHFSAKQLAEAPRSEWKFVPQAKPVPKTKPKTVSSQAAPVEKKAR
                     TSIFRRLFPAPKKTQSFPDPRFFRLKWKKTLEGYISPISIRIWRDWYIPPRHLVFTSG
                     DTLFIYETRKGNLVRAIPLPEKYQEKNQESRYARFSFVYLQDGNRGYVIGTRWLYEIE
                     VKESGGQHILYKGHQMICISDKGEICWEQEDPTTNVPARLDTLPVGPKRDLLFFSSRG
                     GFELKDAERNTLLEQKQVGQNRLSTWFFRLGPSGKGVELVVIGNDIACYQLIIP"
     gene            complement(2328..3929)
                     /locus_tag="PKI64_07090"
     CDS             complement(2328..3929)
                     /locus_tag="PKI64_07090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOE00809.1"
                     /translation="MTAPTYDWIIRGGLVADGRGGELVRADVALRGDRIAAIGELPAT
                     ATHEYDAAGCIVAPGFIDVHTHSDAYLLIEPGAPSKIQQGVTTEITGNCGASAAPRWP
                     GYTMPSDWLEQTYPGEWQSVADYRALLAAQRPAVNSAMLIGHRAIRAAVMGIEPRAAR
                     PEEIARMAQLLADALAEGGMGLSTGLVYAPAMHAQPDEIEALARVVARHGGIYATHMR
                     SEGGALLEAIAEAIQVARATGVRLQISHLKTAGEANWPKLDAAFAAIEAAQAEGLAVA
                     SDRYPYTASCTDLDVILPTWAAQGGRAAILARLRNPADRARIRAEMAAARSDRYWDNV
                     WIGSTRHPANAPYTGQPIARAAAAWNLHPLDAALRFMETDELFTGGIFFGMSEANMWR
                     ILAKPWVMIGSDASLRAPTGPLSHDHPHPRAYGTFGRFLRAAVDGRTVPLGEAIRKMT
                     SLPAAHFRLADRGILRPGAFADVVVFDPATIQDRATFEQPHQFCAGIHAVWVNGAFSL
                     RDGQPLVRAGRYLTPSGNQENSQGH"
     gene            4040..4372
                     /locus_tag="PKI64_07095"
     CDS             4040..4372
                     /locus_tag="PKI64_07095"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOE00810.1"
                     /translation="MSNNTNENLFQGLVISLAAAAMQHMGKTLNPVTQKIEKNLQAAQ
                     GTIDMLDMLAAKTSGNLNEAEGKLLKNILAELKLNYVETLNEKPAEPPAEKPAEAAAE
                     KPAEPPSA"
     gene            4380..6017
                     /locus_tag="PKI64_07100"
     CDS             4380..6017
                     /locus_tag="PKI64_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00811.1"
                     /translation="MRARVFTPKRILTLLLLVAAVGAAVWWVGYIPYDPLAIYRPLPA
                     NATLVGRHLRLPERWPDVLANPLAQALLRTAGVAPEDAADLVADEESFRWFQQLAGRE
                     GTLAYLPSPHGGDDAAWIAVSHLGGRSQKLRWQLALFRVPGFTRIQTFPGRAAWRVAT
                     PDLDPRQVLTIAFGEGILMACLSPDPYAIEAMLAAYDGRSQRLLEREPAFAEFAAQDD
                     RSVPDRFWIRDRSPLADPRAPGITVAVPRLTADALTLVAATAGLDVLPDDRLPTTELT
                     TLARRVGQAPCLGAAVSREALLRVATQTDLPRTERHVLRMILDVMTADRLILVGMDGE
                     MGGRLAWGAIRTLGLAGLRVPTLLLATPAPDAADAHAAIQRVLDASNARYKAAFILNP
                     VSSPAGPIYVLASAGGNEWVDELAPADRPAYAVLDGWLLAASNLAALQKLVAQAPVDA
                     MPEWAEALAGPGALTAWVDLARSGKVARDAIATWSMVQMFLNGGNSQAIREQLNNARA
                     WIDAFAPLAHARLALGRRQNQTTLTLDLGLSAAGAAE"
     gene            6030..6809
                     /locus_tag="PKI64_07105"
     CDS             6030..6809
                     /locus_tag="PKI64_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOE00812.1"
                     /translation="MSDTLVIIPTYNEKENAEAIARAVLAACPEADVLFVDDNSPDGT
                     GQLADQLAAAEPRVHVLHREQKQGLGRAYLAGFRWALERPTYRFIMEMDADFSHDPQA
                     VPQLRAAAQNADLVLGSRYIGGIRVINWPLNRLILSTGAALYVRLITGMPFTDPTGGF
                     KCFRREVLAAMNLAEIHSNGYSFQIEMTHTAWHLGFKIVESPIVFEERREGQSKMSGG
                     IINEALWMVWKLLARCGYRRRPRVVHPQSVAAPPPAGAPAP"
     gene            6806..7690
                     /locus_tag="PKI64_07110"
     CDS             6806..7690
                     /locus_tag="PKI64_07110"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOE00813.1"
                     /translation="MSALRTLLRLLRAKHWTKNAVVLAAFVFAYGDQQQSLTLFTAWK
                     AILAAVAFSLLSSAVYIFNDLRDAPQDRLHPIKRRRPIAAGTVQPLPAAALALGLLVA
                     ALWGAWRIEPALLLVLGSYLLLQVVYTLGLKRVPLVDVLVISVGFVLRALAGGVAIRV
                     PISPWLLVCTMMLALFLALCKRRHEKVNLAGRGTRDALDGYDERLLDLLITMTGTAAL
                     VCYALYTLWPETVHKFGTPWLAATIPFVVFGLFRYMDLVYRQEKGDRPEQLLLTDWPL
                     MLDVALYGLAVLGVLALR"
     gene            7793..9271
                     /locus_tag="PKI64_07115"
     CDS             7793..9271
                     /locus_tag="PKI64_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOE00814.1"
                     /translation="MKIISSINREILARSIQRAKERNIILPTFAQQKDPRKIPAKILE
                     KLKGLDLLAINPLNLFRITWKNDPVTGGFGEGNWVEFPKEITGVDARIVGLVGRYFPT
                     GAHKVGAAYGCLVPRLVSGNFDPTTQKAVWPSTGNYCRGGAFDCALLACPAVAILPEG
                     MSQERFDWLREINTDEIIATPGTESNVKEIYDMCWKLRRERGDSIVIFNQFEEFGNSI
                     WHYQMTGGIIEEIYENHFKTDRSRLAAYCSATGSAGTIAAGDFLRTIHPHIRVVANEA
                     LQCPTLYQFGFGAHRIEGIGDKHLPWVHNIRNTDAIAAIDDEQVMQLLRLFNEPAGQD
                     YLRKQGVSDEIVAQLPFAGISSICNLVSAIWTARYYEMDSRDVIFLPLTDSMDLYSSR
                     QAEMTEQHGPYTTEMAARHYGRYLEACIPSSFRELNYFERKSLHNFKYFTWVEQQAKD
                     SEDLRAMWDEDFWKEVFDPQQVAEWDQLINEFNEATGLLKNL"
     gene            9293..10507
                     /locus_tag="PKI64_07120"
     CDS             9293..10507
                     /locus_tag="PKI64_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOE00815.1"
                     /translation="MSSKLYAEAKKREGAIAEFLKDAASIPSLSSQEGDVVKFLADAM
                     KKMGYDEVVIDGMGNLLGRIGNGPRIIAFDGHCDTVDVGDITLWTKVKPYPAIIKDGK
                     VYGRGVTDQKGGLITSIAGIAMLKELGMVPENLTIWVVASVQEEDCDGLCWQYIVKEG
                     KLRPEVVVSTEPTSLQIYRGHRGRMEIEIETKGVSCHGSAPERGDNAVYKMADIIKDI
                     EKLNERLPPIKPLGKGTVTISHIRSTSPSLCAVADSCTIHLDRRLTTGENEETSVAEL
                     KALPSVQKAKAEVRVLEYSVPSWTGLVYPTKKYYPTWHIEDTTPQVTSAVAAYRNALS
                     EDPVVSYWVFSTNGVSICGMYGIPVIGFGPGHEKFAHFPNEEIDIEHLTRATAFYASF
                     ALEYAKTAAPQK"
     gene            10609..12141
                     /gene="purH"
                     /locus_tag="PKI64_07125"
     CDS             10609..12141
                     /gene="purH"
                     /locus_tag="PKI64_07125"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOE00816.1"
                     /translation="MKIRRALLSVSDKTGLLELARGLAALGVELLSTGGTAATLRAAG
                     LPVTDVSAHTGHPEILDGRVKTLHPRIHGGILQRRDNPADQETCRQLDLPPIDLVCVN
                     LYPFESNPGIETMDIGGPAMIRSAAKNHADVIILTDPTDYPHVLADLQAKGDLPHAAR
                     QALAQKAFARTAAYDTAIAAWLAGQPAPEVPLRYGENPHQTASFRPAVPPPAEANLAT
                     ARILHGKEMSYNNYVDADAALEAVRELHAAPAAVIIKHTNPCGAATGTTLAAALAAAW
                     EGDAVSSFGSVIAVTRPVDLATAQVLKGRFVEALIAPDFAPDALAFLQAKSKDIRLIS
                     LGQPLAAPLPRQLVRHINGGTLTQSADLGATEQWNNVTKALFPDRLRALAQFGITVCK
                     HIKSNAILLAWEYAPGQAAILGMGAGQPNRVDALRKLAVEKARENIALRGLDAAAVLA
                     DTVLVSDAFFPFPDNIEAAAAAGVRHIVAPGGSKRDADCIAACDQHGIAMIFTGCRHF
                     RH"
     gene            12215..13480
                     /locus_tag="PKI64_07130"
     CDS             12215..13480
                     /locus_tag="PKI64_07130"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOE00817.1"
                     /translation="MTTKTFPLTREQTEALTQRFPTPFHLYDEAAIRANVRRLLQAFS
                     ILPDFREYFAVKATPNPFLLKLLADESCGADCSSLAELRLCEAVGLRGDRVMFTSNET
                     PDAEFRAARDLGAIINLDDITHIDALIAATGSLPATLCFRYNPGPLKAGNAIIGHPEE
                     AKYGLTRDQLFTAYSRARDLGVKTFGLHTMVVSNELDAQYHIETARMLFELVVEIHQR
                     LGIRISFINTGGGIGIPYRPEQQAVDYAAIARGARQLYDQIIVPAGLAPLAYNMECGR
                     AITGPYGWLVTRAIRRKETYRSYIGVDASMANLMRPGMYGAYHHLTVLGKENAPATKT
                     YDVVGSLCENCDKFAIQRQLPEIAMGDLLVIHDTGAHGHAMGYNYNGKTRSAELLLRS
                     DGSVIQIRRAETLADLFATLDLPAAAAFH"
BASE COUNT         2602 a         4578 c         4168 g         2403 t
ORIGIN      
        1 atggcgaaac actggatgga ggccgccgag caacgcggca tccccacggc ttttgtagtc
       61 catcaaggcc ggattgcgtg gatcggccat ccgatgcgcg agatgcggga gattcttcag
      121 gaaattaccg ccggtggttt tcaactggag cgggcccagg aacgagcggc agccaaagaa
      181 cagaaggcct tggaaaccca gcgaattgcc gaacttttca gggagtacct caaagctgtc
      241 agggagccgg aagattcggg caaggccgct gaattggctc gccagatcga ggccgccgac
      301 ctgaaaagcc ccgacaccct gaactccatc gcctggaccc ttttgaccag ccgcagcatc
      361 cggcaacgcg atattccctt cgccacccgc ctcgtcaaaa aagcgatgga cctgacccag
      421 gagaaaaacc cggacatcct cgacacctac gcgcgtgcct tgtgggaagc cgggcaacgc
      481 gaggaagcca tcgctgttca gcaaaaagcc gttgatgccg ctcccctgaa gcgcggattt
      541 atggtcaact tggagcgtta tctcgatcaa acccgtccgg agggcccttt gctggcttta
      601 cgcaccgagc ggtttggatc gctggcggcg gtggtgggcg atgccattta tgtcgttggc
      661 ggttattcca atcaggggct ggagggcacc atcgagcgat tcttcgctga ctccaaagcc
      721 acggagaccc tgccggcttc gattcgtccc cggtgtttct tctccggcgc gacaagcggc
      781 ggaaaaatct acatcgttgg cggaatcgtg tggggtgaaa atccgggaaa tctggccgtg
      841 acagcttcct gcgaggaatg ggatccggcg accggggtca tccgttcgtt gccggacatg
      901 ccggtggcgg ttgcccggcc cggcgtggtg gtggtgggcc agcggctgta tgtcatcggc
      961 ggggcacagg gggaaggcga gccccggttt aaaaccgtgc agatttttga catccccacc
     1021 gaaacttgga cgttgggcgc caatctcccg gtggcgcgcg aaggccatgt cttcgaatat
     1081 gagggcaaga tttatgctcc cgggggctac gatggcaatt tggccattcg tgatttccaa
     1141 gtgtatgacc cggcccggaa caggtggcgc ttatggtccc cgttgcccaa taaaatgagt
     1201 gcgcatcatg ggtgcgttgt ggatggtcag ttatatttgt tcggcgacta tgatgagttg
     1261 gatcgcacgg tggtctgcga tctcaaaacg aagaagtggg cgcggatcgc cgtcggctat
     1321 cgtccggccc gccatgcggc cctggctagc accggcaaag acatttttgt gcttggcggc
     1381 aatgttcaaa gctcgagtcc gttcctggct cgcatccagc acttttccgc caaacaactg
     1441 gccgaagcgc cacggagcga gtggaaattc gttcctcaag caaaacccgt gccgaaaacc
     1501 aaacccaaaa cggtatcgtc ccaagcggcg cccgttgaaa aaaaggcccg cacttccatc
     1561 tttcgccggc tctttcccgc gcccaagaaa acgcaaagtt tccctgatcc gcgtttcttc
     1621 cggctgaaat ggaaaaagac actcgagggc tacatttctc caatctctat tcgcatttgg
     1681 agagattggt atatcccgcc tcgccatctg gttttcacct ccggcgacac tttgttcatc
     1741 tatgaaaccc gcaagggaaa ccttgtgcgg gcgattccgt tgccggagaa atatcaagaa
     1801 aagaaccagg aatcgcgcta tgctcgtttt tcctttgtgt atctgcaaga tggcaatcgg
     1861 ggatacgtaa tcggcacgcg atggctctat gagatcgaag tgaaggaatc gggggggcag
     1921 cacatattgt acaaggggca ccaaatgatc tgcatttcgg acaaggggga aatatgttgg
     1981 gaacaggaag accccaccac caatgtcccc gcacgcttgg ataccctgcc cgttggcccc
     2041 aagcgcgacc tgcttttctt cagctccagg ggcggattcg aacttaagga cgcagagcgc
     2101 aatacccttt tggaacaaaa gcaggtggga cagaaccgcc tctctacttg gtttttccgt
     2161 ctggggccat cgggcaaagg cgttgaactg gtggtcattg gaaatgacat cgcctgctat
     2221 caactgatca ttccatagaa gctagcgtgc cgatcccgcc gccggtacaa cggcggccta
     2281 catcttgagc ccgcatgcgc tcttttgcgg cggttgcgtc gctggcgtta atgtccctgg
     2341 ctgttttcct ggtttcccga aggggtgagg tagcggccgg cgcggacgag gggttggcca
     2401 tcgcgcaagg aaaacgcgcc gttaacccag acggcgtgga tgccggcgca aaattggtgg
     2461 ggttgctcaa aggtggcgcg gtcctggatg gtggcgggat cgaagacgac cacatcggcg
     2521 aaggcgccgg ggcgcaggat gccgcgatca gccaagcgga aatgcgcggc gggcaggctg
     2581 gtcattttgc ggatggcttc gccaaggggg acggtgcggc catccacggc ggcgcggaga
     2641 aaacggccaa aggtgccgta ggcgcgcgga tgggggtgat cgtggctcaa ggggccggtg
     2701 ggggcgcgga gggaggcatc ggagccgatc atgacccacg gcttggcgag aatgcgccac
     2761 atattggctt cgctcatgcc gaagaaaatg ccgccggtga aaagttcatc ggtttccatg
     2821 aaacgcaggg cggcatcgag ggggtgcaga ttccaagcgg cggcggcgcg ggcaatgggc
     2881 tggccggtgt agggcgcgtt ggcggggtgg cgggtgctgc cgatccagac attgtcccaa
     2941 tagcggtcgg agcgcgcggc ggccatctcg gcgcggatgc gcgcgcggtc ggcgggattg
     3001 cgcagtcggg caagaatggc ggcgcggccg ccctgcgcgg cccacgtggg gaggatgaca
     3061 tcgaggtcgg tgcaggatgc ggtgtaggga tagcggtcgg aggcgacggc gaggccctcg
     3121 gcttgggcgg cttcgatggc agcgaaggcg gcatcgagct tgggccagtt ggcttcgccg
     3181 gcggttttca ggtgcgagat ttgcaagcgc acgccggtgg cgcgggcaac ttggatggct
     3241 tcggcaatgg cttcgaggag cgcgccgcct tcactgcgca tgtgggtggc gtagatgccg
     3301 ccgtggcgcg caaccacgcg ggcaagggct tcgatttcgt cgggctgcgc gtgcatggcg
     3361 ggggcataga cgaggccggt gctgaggccc atgccgccct cggcgagggc gtcggccagc
     3421 agttgcgcca tgcgggcaat ttcttcgggg cgcgcggcgc gcggttcgat gcccatgacg
     3481 gcggcgcgga tggcgcggtg gccgatgagc atggcggagt tgacggcggg gcgctgggcg
     3541 gccaacaggg cgcggtagtc cgccacgctt tgccactcgc cggggtaggt ttgctccagc
     3601 cagtcggagg gcatggtata gccgggccag cgcggcgcgg cgctggcacc gcagttgccg
     3661 gtgatttccg tggtgacgcc ttgctggatt ttcgaggggg cgccgggttc aatcagcaga
     3721 taggcgtcgg agtgggtgtg gacatcaatg aagccgggcg caacgatgca gccggcggcg
     3781 tcatattcgt gggtagcggt ggcgggcagc tcgccgatgg ccgcgatgcg gtcgccgcgc
     3841 agggcgacat cggcgcgcac gagctcgccg ccgcggccgt cggccacgag gccgccgcgg
     3901 ataatccaat cgtaggtggg ggcagtcatg ggcgggaaga taccaccaag ggcgggcggc
     3961 gtcacgggcg gcgcgtattc cggaattgaa ccggcgggcc gcttcaggta agtacaggcc
     4021 ccaatacgga gaattggcca tgagcaacaa cacaaacgaa aatctattcc aagggctggt
     4081 gatctcgctg gcggcggcgg ccatgcagca catgggcaaa accttgaatc ccgtcacgca
     4141 aaagatcgaa aagaacctgc aagcggcgca gggcacgatt gacatgctcg acatgctggc
     4201 cgccaaaacc agcggcaact tgaacgaggc cgaaggcaag ctgctcaaaa atattttggc
     4261 cgagttgaaa ctgaattatg tggagacgct gaacgagaag ccggcggaac cgcccgcgga
     4321 aaagccggct gaggctgccg cggagaagcc ggccgagccg ccaagcgcgt aaggcgaaca
     4381 tgcgcgcgcg cgtcttcacg cccaagcgaa tcctcacgct gctgctgctg gtcgcagcgg
     4441 tgggcgcggc ggtttggtgg gtgggctaca tcccctatga cccgctggcg atctaccgcc
     4501 ccctgccggc caacgccacg ctggtgggcc gccacctgcg cctgcccgaa cgctggcccg
     4561 acgtgctggc gaatccgctg gcgcaggcgc tgctgcgcac cgcgggggtc gcgccggaag
     4621 atgccgcgga cctcgtagcc gatgaggaat cgttccgctg gttccaacag ttggcggggc
     4681 gcgagggcac gctggcctat ttgccttcgc cccacggcgg cgatgacgca gcatggatcg
     4741 ctgtgagcca cctcggcggg cgcagccaaa aattacgctg gcaactggcg ctgttccgcg
     4801 tgccgggttt cacccgcatc caaaccttcc ccggccgcgc ggcgtggcgc gtggccacgc
     4861 cggacctcga cccgcgccaa gtgctgacca tcgcgttcgg cgaaggcatc ctcatggcgt
     4921 gcctctcgcc ggatccgtat gccatcgagg ctatgctggc ggcgtacgac gggcgcagcc
     4981 agcgcctact ggagcgcgag cccgcgttcg ccgagtttgc cgcgcaggat gaccgctccg
     5041 tgccggaccg gttctggatt cgcgaccgct cgccgctggc cgatccgcgc gcgcccggca
     5101 tcacggtggc cgtaccccga ctgacggcgg atgctctcac gctggtggcc gccaccgccg
     5161 ggcttgatgt gctgccggat gaccgcctgc ccaccaccga gctgaccacg ctggcccgcc
     5221 gcgtggggca ggcgccgtgc ctcggcgcgg ccgtcagccg cgaagcgttg ctgcgcgtgg
     5281 ccacgcaaac cgatttgccg cgcaccgaac gccacgtgct gcgcatgatt ctcgatgtga
     5341 tgaccgccga ccgcctgatt ctggtgggca tggatggcga gatgggcggg cggctggcgt
     5401 ggggcgcgat ccgcacgctg ggtttggccg ggttgcgcgt gcccacgctg ctgctggcca
     5461 cgcccgcgcc ggatgccgcg gatgcgcatg cggctatcca gcgcgtgctg gatgccagca
     5521 acgcgcgcta caaggcggca ttcatcctga atccggtgtc gtcgccggcg ggcccaattt
     5581 atgtgctggc cagcgcgggg ggcaacgagt gggtggatga actcgccccc gccgaccgcc
     5641 ccgcctacgc ggtactggat ggctggctgc tggcggcaag caatctggcc gcgctgcaaa
     5701 agttggtggc acaggcgccg gtggacgcca tgccggaatg ggcggaggcg ctggccggtc
     5761 ccggcgcgct gacggcatgg gtggatttgg cgcgcagcgg caaggtggcg cgcgacgcca
     5821 tcgccacgtg gtccatggtg cagatgttcc tcaacggcgg caattcgcag gctatccgcg
     5881 agcagctcaa caacgcgcgc gcctggattg atgccttcgc gccgctggcc cacgcgcgtc
     5941 tggccctcgg ccgccggcaa aaccaaacca cgctgacgct ggatttggga ttgtccgcgg
     6001 cgggcgcggc ggagtaggat gcgcccatca tgagcgatac gctggtcatt attccgacct
     6061 ataacgaaaa ggaaaatgcc gaggccatcg cgcgcgcggt gctggccgcc tgcccggaag
     6121 ccgacgtgtt gttcgtggat gacaactcgc ccgacggcac gggccaactg gccgaccaac
     6181 tggcggcggc cgagccgcgg gtgcacgtgc tgcaccgcga acaaaagcag ggactggggc
     6241 gcgcctatct ggccggattc cgctgggcgc tggagcggcc gacgtaccgc ttcatcatgg
     6301 aaatggatgc cgacttttcg cacgatccac aagctgtgcc gcaactgcgc gcggcagcgc
     6361 aaaacgcgga tttggtgctg ggctcacgct acatcggcgg cattcgcgtg atcaactggc
     6421 cgctcaaccg cctgatcctt tcaactggcg ccgcgctcta tgtgcggctg atcaccggca
     6481 tgccgttcac cgatcccacc ggcggcttca aatgcttccg ccgcgaagtc ctcgccgcca
     6541 tgaatctcgc ggagattcac tccaacggct actcgttcca gatcgaaatg acgcatacgg
     6601 cgtggcatct ggggttcaag attgtggaat cgcccatcgt gtttgaagag cggcgcgagg
     6661 ggcagtcgaa gatgagcggt gggatcatca acgaggcgct gtggatggtg tggaagctgc
     6721 tggcgcgctg cggctaccgc cgccggccgc gcgtggtcca cccgcagagc gtcgccgcgc
     6781 cgccacccgc cggagcgccc gccccatgag cgccctgcgc acgttgctgc ggctgttgcg
     6841 cgccaagcat tggaccaaga acgccgtggt gctcgcggcg tttgtttttg cctatggcga
     6901 ccagcagcaa agcctgacac tcttcaccgc gtggaaagcc atcctggcag cggtggcgtt
     6961 ttcgctgctg tccagcgcgg tgtatatctt caacgatttg cgcgacgcgc cgcaggaccg
     7021 cttgcatccc atcaagcgcc ggcgacccat cgcggcgggc acggtgcagc ccctgccggc
     7081 ggcggcgctg gcgctcggcc tgctggtggc ggccctgtgg ggcgcgtggc gcatcgagcc
     7141 cgcgctgcta ctcgtgctgg gcagctacct gctgctgcaa gtggtctata cgctgggcct
     7201 caagcgcgtg ccgctggtgg atgtgctggt gatttccgtg ggcttcgtgc tgcgcgcgct
     7261 ggccggcggc gtggccatcc gcgtgccgat cagcccgtgg ctgctggtgt gcaccatgat
     7321 gctggcactc tttttggcgc tgtgtaaacg ccgccacgaa aaggtgaatc tggccggacg
     7381 cggcacgcgc gatgcgctgg atggctatga cgaacgcctg ctcgatttgc tgatcaccat
     7441 gaccggcacc gcggcgctgg tgtgctatgc gctctatacg ctctggccgg aaaccgtcca
     7501 caagttcggc acgccgtggc tggccgccac catcccgttt gtggtctttg gcctcttccg
     7561 ctacatggac ctcgtctatc gccaggaaaa aggcgaccgc cccgaacagt tgctgctgac
     7621 cgactggccg ctgatgctcg atgtggcgct ctacggcctc gcggtgctgg gcgtgctggc
     7681 gctccgctaa aacgcttttt cccgcgcgcc ccattcgcat tgaccccgcc gccttctcca
     7741 gccaaactgc cggcgttcac gtatctttat ttgaaaataa acaggagaac gcatgaagat
     7801 catttcctcg attaaccgcg aaatcctggc ccgcagcatc cagcgcgcca aggaacgcaa
     7861 catcattctg ccgacgtttg cccagcaaaa ggacccgcgc aagattcccg ccaagattct
     7921 ggaaaaactc aaaggcctcg acctgctggc catcaatccg ctcaacttgt tccgcattac
     7981 gtggaagaac gaccccgtca ccggcggttt cggcgaaggc aactgggtgg agtttcccaa
     8041 ggaaatcacc ggcgtggatg cgcgcattgt cggcttggtg ggccgctatt tccccaccgg
     8101 cgcgcacaag gtcggcgcgg cctacggctg cttggtgccg cgattggtga gcggcaactt
     8161 cgaccctacc acccagaagg ccgtgtggcc ctccaccggc aactactgcc gtggcggcgc
     8221 gttcgattgc gccctgctgg cctgccccgc cgtggccatc ctgcccgaag gcatgagcca
     8281 ggagcgcttc gattggctgc gcgaaatcaa caccgatgaa atcatcgcca cccccggcac
     8341 cgaatccaac gtgaaggaaa tctacgacat gtgctggaag ctgcggcgcg aacgcggcga
     8401 cagcatcgtc atcttcaacc agttcgagga gttcggcaac tccatctggc actaccagat
     8461 gaccggcggc atcatcgagg aaatctacga gaaccacttc aagaccgacc gcagccgcct
     8521 cgccgcctat tgcagcgcaa ccggctccgc cggcaccatc gccgcgggcg atttcctgcg
     8581 caccatccac ccgcacatcc gcgtggtggc caacgaggcg ctccagtgcc ccaccctcta
     8641 tcagttcggc ttcggcgcgc accgcattga gggcatcggc gacaagcacc tgccgtgggt
     8701 ccacaacatc cgcaacaccg acgccatcgc ggccattgac gatgagcagg tcatgcagct
     8761 cctgcgcctg ttcaacgagc ccgccggcca ggactacctg cgcaagcagg gcgtcagcga
     8821 cgagattgtg gcgcaactgc cgtttgccgg catctccagc atctgcaacc tcgtcagcgc
     8881 catctggacc gcgcgctatt acgaaatgga cagccgcgat gtcatcttcc tgccgctgac
     8941 cgactcgatg gatctctatt cctcgcggca ggccgaaatg accgagcagc acggccccta
     9001 caccaccgaa atggccgcgc gccattacgg ccgctatctc gaagcctgca tcccttcgtc
     9061 cttccgcgag ctgaattact tcgagcgcaa gtcgctgcat aacttcaagt acttcacctg
     9121 ggtcgagcag caggccaagg atagcgaaga tctccgcgcc atgtgggatg aggacttctg
     9181 gaaggaagtc ttcgacccgc agcaagtggc cgagtgggat caactgatca atgagttcaa
     9241 cgaggcgacc ggcctcctca aaaacctgta aaaacgaaag gagaaaacga acatgagttc
     9301 caagctatat gctgaagcca aaaagcgcga aggcgcaatt gccgagttcc tcaaggatgc
     9361 cgccagcatc ccgtccctga gcagccagga gggcgatgtc gtcaagttcc tggccgatgc
     9421 catgaagaaa atgggctacg atgaagtcgt cattgacggc atgggcaacc tgctcggtcg
     9481 tatcggcaac ggcccgcgca tcatcgcctt cgacggccac tgcgacaccg tggacgtcgg
     9541 agatatcacc ctgtggacca aggtcaagcc ctaccccgcc atcatcaagg atggcaaggt
     9601 ctatggccgc ggcgtcaccg accaaaaggg cgggttgatc acctccatcg ccggtatcgc
     9661 catgctcaaa gagctgggca tggtacccga aaacctgacc atctgggtgg tcgccagcgt
     9721 gcaagaagaa gactgcgacg gcctgtgctg gcagtacatc gtcaaggaag gcaagctgcg
     9781 ccccgaagtg gtcgtttcca ccgaacccac cagcctgcaa atctaccgcg ggcaccgcgg
     9841 ccggatggaa attgagattg aaaccaaggg cgtctcctgc cacggctccg cgcctgagcg
     9901 cggcgacaac gccgtgtaca agatggccga catcatcaag gacatcgaga agctcaacga
     9961 acgcctgccc ccgatcaaac cattgggcaa gggcaccgtc accatcagcc acatccgctc
    10021 cacttcgccg agtctctgcg ccgtggccga tagctgcacc atccacctcg accgccgcct
    10081 gaccacaggc gaaaacgagg aaaccagcgt cgccgagctg aaggccctgc cgagcgtgca
    10141 gaaggccaag gccgaagtgc gcgtgctgga atactccgtg ccgagctgga ccggcttggt
    10201 gtaccccacc aagaagtact accccacctg gcacatcgaa gacaccaccc cgcaggtcac
    10261 cagcgccgtt gccgcctatc gcaacgcgct gagcgaagac cctgtggttt cttactgggt
    10321 gttcagcacc aacggcgtgt ccatttgcgg tatgtacggc attccggtga tcggcttcgg
    10381 cccgggccat gagaagttcg cgcacttccc gaacgaggaa attgacatcg aacacctcac
    10441 ccgcgccacc gcgttctatg ccagcttcgc gctggaatac gccaagaccg ccgccccgca
    10501 gaagtaagct gcgcgcgtgc gccacgcaag ggagcccccc gctcccttgc gtttttttgt
    10561 gccccgtgcg cgcttcccat ttccccgcgc ctgtgccact atgccaccat gaaaatacgt
    10621 cgcgccctat tgagtgtttc cgataaaacc ggcctgctgg agctggcccg cggcctcgcc
    10681 gcgctcggcg tggagctgct ctccaccggc ggcaccgccg ccacgctccg cgctgccggc
    10741 ttgcccgtga cggatgtctc cgcccacact gggcatccgg aaatcctcga tggccgcgtc
    10801 aagaccctcc acccgcgcat tcatggtggc atcctccagc gccgcgacaa ccccgccgac
    10861 caagaaacct gccgccaact cgacctgccg cccattgatc tggtgtgcgt caacttgtac
    10921 ccctttgaga gcaaccccgg tatcgaaacc atggatatcg gcgggccggc tatgatccgc
    10981 tccgccgcca aaaaccatgc ggatgtcatc atcctgaccg atcccaccga ctacccccac
    11041 gtgctcgccg atctgcaagc caaaggcgac ctgccccacg ccgcgcggca ggcgctcgcc
    11101 caaaaagcgt ttgcccgcac cgcggcctac gataccgcca tcgccgcctg gctcgccggc
    11161 caacccgcgc ccgaagtccc gctgcgctat ggcgaaaacc cgcaccaaac cgccagcttc
    11221 cgtcccgccg tccccccgcc cgccgaggcc aacctcgcca ccgcgcgcat cctccacggc
    11281 aaggaaatgt cctataataa ctatgtggac gcggatgccg ccctcgaagc cgtgcgcgaa
    11341 ctccacgccg cccccgctgc cgtcatcatc aagcacacca acccctgcgg cgccgccacc
    11401 ggcacaacct tggccgcggc gctcgccgcc gcgtgggaag gcgacgccgt ctcctcgttc
    11461 ggctcggtca tcgccgtcac ccggccggtg gacctcgcca cagcccaagt cctcaagggt
    11521 cgcttcgtgg aagcgctcat cgcgcccgat ttcgcccctg atgccctcgc cttcttgcag
    11581 gccaaatcca aggatatccg actgatttcc ctcggccagc cactcgccgc gcccctgccg
    11641 cggcaactgg tccgccacat taacggcggc acgctcaccc aaagcgccga cctcggcgca
    11701 accgaacagt ggaacaatgt caccaaagcc cttttccccg accgcttgcg tgcgctcgcc
    11761 caattcggca tcaccgtgtg caaacacatt aaatccaacg ccatcctcct cgcctgggaa
    11821 tacgcccccg gccaagccgc catcctcggc atgggcgccg gccaacccaa tcgggtggat
    11881 gccctgcgca aactggccgt ggaaaaagcg cgcgaaaaca tcgccctgcg cggcctcgat
    11941 gccgccgccg tgctggccga taccgtgctg gtatctgatg ctttcttccc gttccccgac
    12001 aacatcgagg ccgccgccgc ggccggcgtc cgccatattg tcgcccccgg cggctccaag
    12061 cgggatgccg actgcatcgc cgcctgcgat cagcacggta tcgcgatgat cttcaccggc
    12121 tgccgccatt tccgtcatta accgctccgg tttcccgctt gccttcaccc cgcgccaccc
    12181 ctaacctgat tccccaatac agcagagcaa cgccatgacc accaagacgt ttccccttac
    12241 gcgcgaacaa accgaagccc tcactcagcg cttccccacc ccctttcacc tctacgacga
    12301 agccgccatc cgcgccaacg tccgccgcct cctgcaagcg ttttccatcc tgcccgactt
    12361 ccgcgaatac tttgccgtca aggccacccc caaccccttc ctactcaaac tactggccga
    12421 tgaaagttgc ggcgccgatt gctccagcct cgccgagctc cgcctgtgtg aagctgtcgg
    12481 cctgcgcggc gaccgcgtca tgttcacttc caacgaaacc cccgatgccg aattccgcgc
    12541 cgcccgcgac ctcggcgcca tcatcaacct cgacgacatc acccacattg acgccctcat
    12601 tgccgccacc ggttcgctgc ccgccaccct gtgcttccgc tacaaccccg gccccctcaa
    12661 agccggcaac gccatcatcg gccaccccga agaagccaaa tacggcctca cccgcgacca
    12721 gcttttcacc gcctattccc gcgcgcgcga cctcggcgtc aaaaccttcg gcctgcacac
    12781 catggtcgtc tccaacgaac tcgatgcaca ataccacatc gaaaccgccc gcatgctctt
    12841 cgagctggtg gtggaaatcc accagcgcct cggcatccgc atctccttca tcaacaccgg
    12901 cggcggcatt ggcatcccct accgtcccga gcagcaagcc gtggactatg ccgccatcgc
    12961 ccgcggcgcg cgccagctct acgaccaaat catcgtgcct gccggcctcg cccccctcgc
    13021 ctacaacatg gaatgcggcc gcgccatcac cggcccctac ggctggctcg tcacccgcgc
    13081 catccgccgc aaggaaacct accgctccta tatcggcgtg gatgcctcca tggccaacct
    13141 catgcgtccc ggaatgtacg gcgcctatca ccacctcact gtgctgggca aagaaaacgc
    13201 ccccgccacc aagacctacg atgtcgtcgg ctccctctgc gaaaattgcg acaaattcgc
    13261 catccagcgc caactccccg aaatcgccat gggcgatctg ctcgttatcc acgacaccgg
    13321 cgcgcacggc cacgctatgg gctacaacta caacggtaaa acccgctccg ccgaactcct
    13381 cctgcgctcc gatggctccg tcatccaaat ccgccgcgcg gaaaccctcg ccgacctctt
    13441 cgccaccctc gacctccccg ccgccgccgc cttccattga ccctacggtc agattgttgg
    13501 ctggaacggt cgtagacctt cccgccgctc ccggcgggaa ggtttcatcc cacaaccatt
    13561 ctcgctctac aggaataact tttacgagtc agcggctggc gcaagagttt caatccacgc
    13621 gccccgtttt tcgttgaagg gaaacgcaat agcttcgccc aaatccaaaa tacgagttaa
    13681 aactaaaccg ttacaagtcc tgagttcaac aatgggacac ccgaagctat ttatgggggc
    13741 agatcaagtc g
//
LOCUS       DAOPNP010000066        13311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5411_length_13311_cov_8.240646, whole genome shotgun sequence.
ACCESSION   DAOPNP010000066 DAOPNP010000000
VERSION     DAOPNP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5411_length_13311_cov_8.240646"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..314
                     /locus_tag="PKI64_07135"
     CDS             42..314
                     /locus_tag="PKI64_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00818.1"
                     /translation="MWNYPDNTATLQMIDAYDPETEFLVLFDTDEGNTRTIRIRTPEG
                     GRNPKGLWFFEMLRRIQEEPESVPDDLPAWFWGALDRLDKMKKQNE"
     gene            complement(411..1583)
                     /locus_tag="PKI64_07140"
     CDS             complement(411..1583)
                     /locus_tag="PKI64_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOE00819.1"
                     /translation="MHSSPAELFANYVMPTYAPGLTLVRGAGVNVWDSAGKEYLDFTA
                     GIAVCNTGHCHPAIRAALADQAGKLIHCSNLFYNELQPRLAQKISQLSLGGKVFFCNS
                     GAEANECLIKLARKWGSATGRHEIITMKNSFHGRTLATMTATGQTKYQNGFAPLVPGF
                     VYAEFNNLDSVRAAITPATVAVLCEAIQGEGGILPATPEFLTGLRELCTAKNLLLFFD
                     EVQTGIGRTGQWFGYQHFGIRPDGISMAKALGSGFPIGAAAVTPELSNVFQPGNHAST
                     FGGTPLASAVALATLETIEKENLLANANQMGARLMDGLRRLATTNPLITAVRGKGLMI
                     GLELNAPAKPYEAKARENGLLCIATGEHVLRFVPPLVVNADQIDRALAILTQSLTP"
     gene            complement(1573..2454)
                     /gene="argB"
                     /locus_tag="PKI64_07145"
     CDS             complement(1573..2454)
                     /gene="argB"
                     /locus_tag="PKI64_07145"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOE00820.1"
                     /translation="MKDAIQKANVLIEALPHIQRFRGETVVVKFGGSSMDNPDVVRDI
                     LKDIAFMSCVGLRPVIVHGGGKAINARMKEQGLTATFVKGLRVTDPQTMEIVEQVLNR
                     EVNPELVAALNSLGCKARGIHGEDIISVRQHTVTDPSTGEKFNWGLVGEILQVDIEPV
                     KAFLEADLTPVITPLGADANRQVYNINADAAAAGLAERLQARKLVFLSDVPGLLADPK
                     DRASLMSTLNLSEIESLIERGIIGGGMLPKISGALKALKAGVRKTHIIDAALPHSLLL
                     ELFTDKGVGTEIVNDAL"
     gene            complement(2461..3666)
                     /gene="argJ"
                     /locus_tag="PKI64_07150"
     CDS             complement(2461..3666)
                     /gene="argJ"
                     /locus_tag="PKI64_07150"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOE00821.1"
                     /translation="MKTTSLKFSAAATLPAGFRAAGIVAGLKKSGKPDMAMFYSDQPA
                     TLAGTFTTNQVRSATVRLDAARVAKVGVARGIVVNSGQANACTGKQGLRDAEAMAAHA
                     AKQFRIPAKHFLVCSTGRIGVPLRMDLIVPGIEKLAQSLAPTGGEAAAAGIMTTDTRP
                     KRFTVPFKVNGRACRLTGIAKGAGMIQPCMATMLAFLLTDAHVDRRALQRALKAAVDR
                     SFNRISVDGDQSTNDTVLLLANGCAGNALLKPGHPDWPAFVAALEGVTFRLAMEMVRD
                     GEGAQKFVTVTVQGARSVADAEAAVRSVANSFLVKTSWVGTYPNWGRLMDALGYSAAK
                     VDESKVEIRYNRLLAVRNGLKAGASIEQLSRVVAQKEFAITIDLHLGRHAATVYSCDC
                     TEEYVRINK"
     gene            complement(3737..4141)
                     /gene="rpsI"
                     /locus_tag="PKI64_07155"
     CDS             complement(3737..4141)
                     /gene="rpsI"
                     /locus_tag="PKI64_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE00822.1"
                     /translation="MATKNVEIESIATGRRKTAIARVRLTKGSGSITVNGRKFEEYFP
                     TTDLRLIVEAPFKVVEGVGKYDVIALCEGGGVPGQAGALRHGIARALIQADDNFRPAL
                     KKAGFLTRDPRMKERKKPGQPGARRRFQFSKR"
     gene            complement(4157..4585)
                     /gene="rplM"
                     /locus_tag="PKI64_07160"
     CDS             complement(4157..4585)
                     /gene="rplM"
                     /locus_tag="PKI64_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE00823.1"
                     /translation="MKTTLPKESEIKRAWHLIDATDKPAGRLAVEIARLLRGRHKPDY
                     TPHVDMGDFVVVINSAKVGLSGARKEDQKVYAKYTGFQSGYTETSAGTVRAKNPNYIL
                     WHAVRGMLPKNKLSRQLIKRLKLYPGAEHPHLPQNPQPIG"
     gene            4930..10695
                     /locus_tag="PKI64_07165"
     CDS             4930..10695
                     /locus_tag="PKI64_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOE00824.1"
                     /translation="MKWMQSCRRLLGWGLGLGMVLGGLTAAGGQTTVVAWDSATNSVY
                     SGGNWSDGQNGGTGFAAWEIAIAGGNYRGSYSGNPEQKGVGGMNSPAWGLYCHSFGNS
                     VTARRPFSQDMVVGDTLSFQWGFNLGSGGTGSKGFTIYAGATPLVTATNGAGTEVYVN
                     GVDSGFGDGTNAMTWVFEYVNATTLVVTANGRDGVSSYSNDVAIASAPDALTFFMTRQ
                     GDDTQAREPFYNDLLIQHTDNREAPEILSWPAASPLAWGDRLADAGLTGGVAGIDGVF
                     EWVNEEVIPGAGTTLQEVRFVPADTDTYSVVVSQVLVTAVGGIYINEVQSSNGLTIED
                     EDGDTPDWIELYNATDAAIPLAGWGLSDSAKKPFKWVFGEVSIQPHEYLIVFASDKDR
                     RAITNGNYLHTSFGISSDGEDIVLTDSHTNRMDLVPAKVISRDYSLGRYPNGTGDLWF
                     FQAPTPGAANHNNGYLTDLPPVAFSVPGGYYTSTVSLELSTTEEDATIRYTLDGSEPT
                     ASSPAYSNALSIGVRSTPNDLSAIQTTYGTGGEDDWEAPDGSVFKFYTVRARIFKDGS
                     PGPHTTHSYIVTPAGTNRYTLPVVSIATDKANLFDNTIGIYTPVNNNMMQKGAAWERT
                     GTIEYFEPDGTLAFSGNVGLRLHGNTTRSRPRKAIRVYARGPGKFKYKLFDDLNLATF
                     ETFILRNGGNDWGQGLLRDLYAQSLTANPTLDRQHGRPVIVFLDGEYWGIHDLRERFD
                     EGYIDNHYGLGELEYVQVEIDRDVGGNPNVPVYDSGNRAWGADYSNLWAYVKNHNLTV
                     ATNYAVVQDWMDLDNFADFFQAHIFVGNSDWPGNNIRAWRSIAQVRDEGAPHGLDGRW
                     RYMIYDMDFAFGLDYSYVPGNKNFDIYKKNFGLVDQFNMLAYVQNAEQDGATWANEPD
                     ATVMYRKLMANPEFRQHFMLRFSDQLNTSYSRAWATSQWARVSATIQPELAEHVARWR
                     QPTNWAQHGERIEYFAQQRPAAVWTHLQDVLKLDWRVPVTVEANDEQGLVRINTIELS
                     DQTPGFAGYPWTGTYFTNYPVTLTAVAKPGYEFVEWRAALAADVVIAADSANNYTRWN
                     NGNNRGYGFGAWTLSKSGNSGYFLDQGGQWGLWANNGGRAEIERPLSTALGVGQTIGF
                     RMQNRWVTSGGSVGVSLRNAADQTLWSITFTNGHNFYNIAGQTNVLAYTTAPIDVELT
                     LTAANTYTARITAGGAEPVILSGNLAGSGSIEKIYAVNHNAGDTSNCDFFVNDLRVFA
                     RGTVGAKTSYSTNETIAVTLTGASQLEAVFEEVAAPDYKLIHYWNFNDSGALLAPTFT
                     KVAGAGMAFDLPEGSEAVAGTGQDFFAENARFGDEASSHLRVNNPLGVVMDVAMPTRG
                     YEDIVVKFEVRRSGKGAGTLNLYYTLDGEDYEPLQTVTTYDAAPNLITLDFSAMTSVD
                     NNTNFALRIEFEQGGGGNAGNVRFDNWTVEGLALEGFNLPPEIVAPVGHQSLMEGGGS
                     IQVDLNDVFQDPDYDALAFEASSSRSNLVALNLAGSMLTVIPQARGGATVTVTADDGE
                     NEPVSSTFYVLVNPTAHVLANAAYTFGEWETNEPAFSYPTNMLFLQSAKNDPGLNDEL
                     LYAYMIPAADAALPEDAEYPYAASSRTRINGLGTNGIAFINTGRGRDVGGALVALDTR
                     GVAEAPVTWLGGTVLPNKRIYAIRLQYRVGATGAFTDVLDDNGQLVEYLRSETVDHSA
                     TLGPVSLPAAALEQEYMQVLWRYYYQTEGSGARAQLRLDDLMIANGPPPLPTGFAAWQ
                     GAEFSPEELANPAISGPLADPTDSGLANLMRYALGLTRNADFHQALPFATVTGETPDT
                     AAVFRHRRLLTPNSGVEYIPEYAEGLITAQWHTAITNSDLIWQGATPTGDGLTEEVEY
                     LVPAGSLQPLRFFRLKVRLME"
     gene            10703..13234
                     /locus_tag="PKI64_07170"
     CDS             10703..13234
                     /locus_tag="PKI64_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE00825.1"
                     /translation="MKPRTALSGWRQFFWIGWLALGSLPASAATWYVATNGSDAAAGT
                     LAAPFRSVNHALQQAVSGDVVEVRGGAYQDTSEVRFRRPGITLRSYSNEWARLVAPLN
                     NENDFSSCVWVDADAKGTVLSRLEIQGGYYYGVVLQTKWDWGDPADRSGASGVIIQDC
                     VIHDTGRDAIKITPNCDDVVIERCEIYRSGVGPANQHAQNAEGIDCVNSDRLTVRDCV
                     IRDAFSTGIYLKGGATDGLIERTRVERCGAGGILLGFDTSPEFFDVEANPSYYENIRG
                     TVRNCLVRDTAWEGIGIYAASNPVVFNNTLLNVCTDAVHSAIYFGLSYQDWEPEAGRP
                     ATVLPQIYNNLVIQPAGFDDEMVEIRYADELGGLSALQGWPAMDYNLYFVAGGGQPWF
                     TDRRPGTELERGTLAQWRAHSGADAHSRVANPLALGGEHPLLLLGSPGLNAGTNAAWM
                     AGVTDLVGQPRILGGQVDIGAYETGTNDWAQLIIGHAALAAATNPPPAAAARVGQLRW
                     FFTHASVGGNLITGLNGLYQENAARYPLQMYNYDGQNGDGDYHGAVPTAGTSDAPDYR
                     AAAVPSVTANGRIYECQRGNPSWENKLVCFSNSVVQSGWRFPKVNVALDKFCWIDPAA
                     DPAAYCATMAGLAARYPQTLLVYMTMPLTTETAGSENDARNDFNRYVRAFCRTTGAWL
                     LDLADITAWTAAGSEQTYVAGGVTNQRLVAAYAVDAGSGDFHLNATGRRRGALGWHAL
                     AQALFQTDRDGDGVTDGDELIAGTVPTDPDSFLALVQDTGGAGERVLRWAGVTNRWYN
                     VQESTALGTNSAWVTRAAHLPAAAINLHTVAVTEASSFWRLTVEQ"
BASE COUNT         2535 a         3716 c         4440 g         2620 t
ORIGIN      
        1 aggcgcccct gttcagacca tggccggact gatccatctc tttgtggaat tatccggaca
       61 ataccgccac ccttcaaatg attgacgcct acgatcctga aactgaattc ctcgtgctat
      121 tcgataccga cgaaggcaat acccgaacta tccgcatcag aacgcctgaa ggtggtcgaa
      181 atccaaaggg cctctggttt tttgagatgt tgaggcgtat tcaggaagag cctgaatcgg
      241 tgccagatga tctcccggct tggttctggg gagccttgga cagactggat aagatgaaga
      301 aacaaaacga gtgattgaag aatagagcca catcctcgga aaagaaaaac gattactttt
      361 gggcgtgctt tttcggagaa agagatcgag cagccacgtc agggagggcg ttatggggtg
      421 agggattggg tgaggatggc gagggcgcgg tcaatttggt cggcgttgac gacgaggggg
      481 gggacgaagc gcaggacgtg ttcacccgtg gcgatgcaga ggagtccgtt ttcgcgggct
      541 ttggcttcgt agggtttggc gggggcgttg agttccaagc caatcatgag ccctttgccg
      601 cgcacagcgg tgatgagggg attggtggtg gcaaggcggc gcaggccatc catcaggcgc
      661 gcgcccattt ggttggcgtt ggcgagcagg ttttcttttt cgatggtttc aagggtggcg
      721 agggccaccg cactggcgag cggggtgccc ccgaaggtgc tggcgtgatt gccgggttgg
      781 aagacattgg aaagttccgg ggtgacggcg gcagcgccaa tcgggaagcc gctgcccagc
      841 gccttggcca tggagatgcc atcggggcgg atgccgaaat gttgatagcc aaaccattgg
      901 ccggtgcggc cgataccggt ttggacttca tcgaagaaca gcagcaggtt tttggcggtg
      961 cacagctcgc gcaggccagt caggaattcg ggggtggcgg gcaggatgcc gccttcgcct
     1021 tgaatggcct cgcagaggac cgcgacggtg gcgggggtga tggcggcacg gacggagtcg
     1081 aggttattga actcggcata gacaaacccg ggcacgaggg gagcaaaccc attttgatat
     1141 ttggtctggc cggtggcggt catggtggcg agggttcggc catgaaagga gtttttcatc
     1201 gtgatgattt cgtggcggcc ggtggcggag ccccacttgc gggcgagctt gatgaggcat
     1261 tcgttggctt cggcgccgga gttgcaaaag aacaccttgc cgcccagtga aagttgcgag
     1321 attttttggg cgaggcgcgg ttgcagctcg ttgtagaaaa ggttggagca gtggatcagt
     1381 ttgccggctt ggtcggcgag ggcggcgcgg atggccgggt ggcagtggcc agtgttgcag
     1441 acggcaatgc cggcggtgaa atcgaggtat tccttgccgg cgctatccca gacgttgacg
     1501 ccggcgccgc gcacaagggt caggcccggc gcgtaggtgg gcatcacgta gttggcaaac
     1561 agttccgcgg ggctagagtg catcgttgac gatctccgtg ccgacacctt tgtcggtgaa
     1621 cagttcgagg aggagggagt gggggagggc ggcatcaatg atgtgcgttt tacgcacgcc
     1681 ggccttgagg gccttgagcg cgccgctgat tttcggcagc atgccgccgc cgataatgcc
     1741 gcgctcaatc aggctttcga tttccgacag gttgagggtg ctcatgaggg aggcgcgatc
     1801 cttggggtcg gccaagaggc cggggacgtc ggaaagaaaa accaatttgc gcgcttggag
     1861 ccgttcggcc aggccggcgg cggcggcatc ggcattgatg ttatagacct gccggttggc
     1921 atccgcgccg agcggggtga tgacgggggt gaggtcggct tcgaggaagg ctttgacggg
     1981 ttcgatgtcc acttgcaaga tttcgccgac gagcccccag ttgaactttt cgccggtgct
     2041 gggatcggtg acggtgtgct ggcggacgga aatgatgtcc tcgccatgga tgccgcgggc
     2101 tttgcagccg agcgagttga gcgcggcgac caactccgga ttcacctcgc ggttgagcac
     2161 ttgctctacg atttccatgg tttgcgggtc ggtgacgcgc aggcccttga cgaacgtggc
     2221 ggtgaggccc tgttccttca tccgcgcatt gatggccttg ccgccgccgt gaacaatcac
     2281 ggggcgcaag cccacgcagc tcatgaaggc gatatccttg aggatgtcgc gcaccacgtc
     2341 ggggttatcc atggaggaac cgccgaattt caccacgacg gtttcgccgc ggaaccgctg
     2401 gatgtgcggc agggcttcga tgaggacatt cgctttttgg atggcgtctt tcatgggtaa
     2461 ttatttgttg atcctaacat actcttcggt gcaatcgcac gaataaaccg tggcagcgtg
     2521 gcgacccagg tgcagatcaa tcgtgatggc aaactctttt tgggccacga cgcgcgagag
     2581 ctgctcgatg gacgcgccgg ccttgaggcc attccgcacg gccagcaggc ggttgtagcg
     2641 aatttccacc ttggattcat ccaccttggc ggcggagtag cccagcgcat ccatcagccg
     2701 cccccagttg gggtaggtgc ccacccacga ggttttcacc aggaacgaat tggccaccga
     2761 gcgcacagcg gcttcggcat cggcgacgct ccgcgcgccc tgcaccgtga ccgtgacgaa
     2821 cttttgcgcg ccttcgccgt cgcgcaccat ttccatggcg aggcggaagg tgacgccttc
     2881 gagcgcggcc acaaaagcgg gccaatcggg gtggcccggc ttgaggagcg cgttgccggc
     2941 gcagccgttg gccagcagca ggacggtatc gttggtgctt tggtcgccat ccacggaaat
     3001 ccggttgaaa gagcggtcca cggcggcttt caacgcgcgt tgcagcgcgc gccggtccac
     3061 gtgggcatcg gtcagcagaa acgccagcat ggtggccatg cagggctgga tcatgccggc
     3121 gcccttggcg atgcccgtca accggcaggc gcggccattc accttgaacg gtacagtgaa
     3181 gcgcttgggc cgggtatcgg tggtcatgat gccggcggcc gcggcctcgc cgccggtggg
     3241 ggcgagggat tgcgccagtt tttcgatgcc gggcacgatg aggtccatgc gcagcggcac
     3301 gccgatgcgg ccggtggagc acaccagaaa atgtttggcg ggaatccgga attgcttggc
     3361 ggcgtgggcg gccatggctt cggcatcgcg cagcccctgc ttgccggtgc aggcgttggc
     3421 ttgaccgcta ttgaccacga ttccccgtgc cacgccaacc ttggctacgc gcgcggcatc
     3481 cagccggacg gttgcggagc gcacttggtt ggtggtgaag gtgccggcca gcgtggccgg
     3541 ctggtcggaa tagaacatgg ccatgtcggg cttgccgctc tttttgaggc cagccacgat
     3601 tccggcggcg cggaagccgg ccggcagcgt ggcggcggcg ctgaatttca aggaagttgt
     3661 tttcattggg ggaccacctt tcataagaaa aaccccggac aaggtgcccg gggttgaatc
     3721 gtccagcgga aacgacttag cgtttcgaga actggaagcg gcgccgggcg ccgggctggc
     3781 cgggcttctt gcgctctttc atgcgcgggt cgcgggtgag gaagccggcc tttttcaggg
     3841 cggggcggaa gttgtcgtcc gcctgaatca acgcgcgggc gatgccgtgg cgcagggcac
     3901 cggcctggcc gggcacaccg ccgccttcgc acagcgcaat gacatcatat ttgccgacgc
     3961 cttccaccac cttgaacggc gcttcgacaa tcaggcgcag gtcggttgtg gggaaatatt
     4021 cttcgaactt gcggccgttg accgtgatgg agccggagcc cttggtcagg cggacgcggg
     4081 cgatggccgt tttgcggcga ccggtggcga tggattcgat ttcaacgttt ttcgtggcca
     4141 tgttgcgtct ccgaaattag ccgatgggct gggggttttg gggcaggtgc gggtgctcgg
     4201 cgcccgggta cagcttgagg cgcttgatca actggcggct cagcttgttt ttgggcagca
     4261 tgccgcggac ggcgtgccag aggatatagt tggggttctt ggcgcggacg gtgccggcgc
     4321 tggtttcggt gtagccgctt tggaagccgg tatatttggc gtacaccttc tggtcttcct
     4381 tgcgggcgcc gctcaggccc accttggcgg aattgatcac cactacaaaa tcgcccatgt
     4441 ccacgtgggg ggtatagtcg ggcttgtggc ggccacggag cagccgggca atttccacgg
     4501 ccaagcggcc ggcgggcttg tcggtggcat caatcagatg ccacgcgcgc tttatttcag
     4561 attctttcgg caatgtcgtc ttcataatcc ttcaatatcc aaacaatgag cggcgaacag
     4621 tagcgcgact ggcgcgcgac tgtcaacgcg gttctttcca tgttgcaatg tgaaacaacc
     4681 ccgtgccgga gggatcggat tatccgacga cagaggactc tttgcaccac gttgccggcg
     4741 gaaatccgcc cgcagccgca ccaccggtta ccgccggcag ccagcccgct gcgcaaaaga
     4801 aacaggcgcg caccgtcgca cacttggccg gttctgtcaa ttcttccgca atattttttt
     4861 tgtgttaagg gaaaaaaggg tggccatggg ggctcccggc ggggtaaaat gttttattgg
     4921 agtgtgatta tgaaatggat gcaatcttgc cgccggctgc tgggctgggg cctcggactg
     4981 gggatggtac tgggggggct aacggcggcg ggggggcaaa ccacggtggt ggcatgggac
     5041 agcgcgacca atagcgtgta ttccggtggc aactggtccg acggccaaaa tggcgggact
     5101 ggctttgcgg cgtgggagat tgccatcgcg ggcggaaatt accgtggttc ctattccgga
     5161 aatccggagc agaagggggt gggagggatg aactcgcccg cgtgggggct ctattgccac
     5221 tctttcggta attccgtgac ggcccgccgc ccgttcagcc aagacatggt cgtgggcgat
     5281 acgttgagct tccagtgggg gttcaatttg ggttccgggg gaaccggcag caaggggttc
     5341 acgatttatg cgggagcaac gccgctggtg acggctacca acggcgccgg caccgaagtc
     5401 tatgtgaatg gggtggacag tggttttggc gatggcacca acgccatgac ctgggtgttt
     5461 gagtatgtga acgccaccac gctggtcgtg accgccaacg ggcgcgacgg ggtgagttca
     5521 tatagcaacg atgtggccat cgccagcgcg cccgacgcgc tgacgttttt catgacccgg
     5581 cagggggatg acacccaagc gcgcgagccg ttttacaacg acctgctgat tcagcatacc
     5641 gacaatcggg aagcgccgga aatcctctcg tggcccgcgg ccagcccgct ggcgtggggc
     5701 gaccggttgg ccgacgccgg gctgacggga ggagtcgccg gcatagacgg ggtatttgag
     5761 tgggtgaacg aagaggtgat tccgggcgcg ggcacgacgc tccaggaagt acgttttgtg
     5821 ccggcggata ccgacaccta ctccgtggtt gtgagtcaag ttttggtgac ggccgtcggc
     5881 gggatttaca tcaatgaagt gcagtcctcc aacgggttga ccattgaaga cgaagacggc
     5941 gacacgccgg actggatcga gctctacaac gccaccgatg cggccatccc gttggcgggg
     6001 tgggggctgt cggacagcgc caagaagccg ttcaaatggg tatttggcga ggtgagcatc
     6061 cagcctcatg aatatctgat tgtgtttgcg tcggacaaag accgtcgggc gatcaccaac
     6121 ggcaactatt tgcacaccag ttttggcatc agctccgatg gtgaagacat tgtgctgacc
     6181 gactcgcata cgaatcggat ggacctggtg cctgccaagg tgatcagccg ggattactcg
     6241 ctgggacggt atccgaacgg cacgggcgac ctgtggtttt tccaagcccc cacgccgggc
     6301 gcggccaacc acaacaacgg ctatttgacg gacctgccgc cggtggcgtt ctccgtgccg
     6361 ggcggctatt acacctcgac ggtgtcgctg gagctatcca ccaccgagga agacgcgaca
     6421 atccgctaca cgctggatgg cagcgagccg acggcaagct cgccggctta ttccaacgcg
     6481 ctgagcatcg gcgtgcgatc aacacccaat gatttgtcgg cgattcagac gacctacggc
     6541 accgggggcg aggatgattg ggaagcgccg gatggttccg tgttcaagtt ctacaccgtg
     6601 cgcgcgcgca ttttcaaaga cggctcgccg ggaccgcaca cgacgcattc gtatattgtc
     6661 acgccggccg gcaccaaccg ctacacgttg ccggtggtgt ccattgcgac ggacaaggct
     6721 aacctgtttg acaacaccat cggcatctac acgccggtga acaacaacat gatgcagaag
     6781 ggtgcggcgt gggagcggac gggcacgatt gagtatttcg agccggacgg cacgctggca
     6841 ttcagcggca acgtcggatt gcgcctgcac ggcaacacca cgcgcagccg cccgcggaag
     6901 gccatccggg tttatgcgcg cgggccgggc aagttcaaat acaagctgtt cgacgacctg
     6961 aatctcgcca cctttgaaac gttcattctg cgcaatggcg gcaacgactg ggggcagggg
     7021 ctgttgcgcg atttgtatgc gcaatcgctg actgccaatc caacgctgga ccgccagcac
     7081 gggcgccccg tgattgtgtt tttggatggt gaatattggg gcatccacga cctgcgcgag
     7141 cggtttgatg agggctatat tgacaaccat tacgggctgg gcgagctgga gtatgtgcag
     7201 gtggagattg accgcgacgt gggaggaaac cccaacgtgc cggtatatga cagcggcaac
     7261 cgcgcttggg gcgcggacta ttccaacctg tgggcctatg tgaagaatca caatttgacc
     7321 gtggcaacca actatgccgt ggtgcaggac tggatggatt tggacaactt tgccgatttc
     7381 ttccaagcgc acatttttgt ggggaactcg gattggcccg gcaacaacat ccgcgcgtgg
     7441 cgttccatcg cgcaagtgcg cgatgaaggc gcgccccacg ggctggacgg gcgctggcgc
     7501 tacatgatct acgacatgga tttcgcgttt ggcctggact acagctatgt gccgggcaac
     7561 aagaacttcg acatatacaa aaagaatttc ggtctggtgg accagttcaa catgctcgcc
     7621 tatgtgcaga acgccgaaca ggacggcgcc acgtgggcca atgaaccgga tgcgacggtg
     7681 atgtatcgca agctgatggc caacccggag ttccggcagc attttatgtt gcggttcagc
     7741 gaccagttga acacgtccta cagccgcgct tgggccacca gtcagtgggc gcgcgtgagc
     7801 gcaaccattc agccagaact ggcggaacac gtggctcgct ggcgccagcc gacgaattgg
     7861 gcgcagcacg gggagcgtat cgagtacttt gcgcagcaac gcccggccgc ggtttggacg
     7921 cacttgcaag atgtgttgaa actggattgg cgcgtgccgg tcacggtgga ggccaacgat
     7981 gaacaaggcc ttgtacggat caatacgatt gagctgtccg atcagacgcc cggctttgcg
     8041 gggtacccgt ggacgggcac ctatttcacc aattatccgg tgacgctgac ggccgtggcc
     8101 aagccgggct acgagtttgt cgaatggcgg gccgcactgg cggcggatgt tgtcattgcc
     8161 gcggactcgg caaataacta cacccggtgg aacaacggca acaaccgggg gtatgggttt
     8221 ggcgcttgga cgctgtccaa gagcggcaat agcggctatt tccttgacca gggcgggcag
     8281 tgggggctgt gggcgaataa tggcgggcgg gcggagattg agcgtccgct cagcacggca
     8341 ttgggcgtgg ggcaaacgat cggcttccgc atgcaaaacc gctgggttac ctccggcggg
     8401 agcgtggggg tcagcttgcg caatgccgcc gaccaaacac tctggtccat cacgttcaca
     8461 aatgggcaca acttctacaa catcgccggg caaaccaatg tgcttgccta tacaaccgcg
     8521 ccgattgacg tggaactgac gctgaccgcg gccaacacct acaccgccag aatcaccgcc
     8581 ggcggtgcgg agcctgtcat tctttcgggc aacctcgccg gcagtggcag cattgaaaag
     8641 atttacgcgg tcaaccacaa cgcgggcgac accagcaact gcgacttctt tgtgaacgac
     8701 ctgcgggtct ttgcgcgcgg gacggtcggc gcgaagacaa gctacagcac gaacgaaacc
     8761 atcgcggtga cgctgaccgg ggcgagccag ttggaagctg tcttcgagga agtggcagcc
     8821 ccggactaca agttgatcca ctattggaac tttaacgaca gcggcgcatt gttggcgccc
     8881 acattcacca aggtggccgg cgcaggtatg gcgtttgacc ttccggaggg ttcagaagcg
     8941 gtggccggca ccgggcaaga tttcttcgca gaaaacgccc ggttcggtga tgaggcatcg
     9001 tcacatctgc gcgtcaacaa tccgcttggg gtggtaatgg atgtcgccat gcccacgcgc
     9061 ggttatgagg acattgtggt gaagtttgag gtccgccgct cgggcaaggg ggcggggaca
     9121 ttgaatctct attacaccct cgatggcgag gattatgagc cgctgcaaac cgtgacgacg
     9181 tatgacgcgg cgcccaatct gatcacgctc gatttcagcg ccatgaccag cgtggacaac
     9241 aatacgaatt tcgctctgcg gatcgagttt gagcagggcg ggggtggcaa cgcaggcaac
     9301 gtgcgctttg acaactggac ggtggagggc cttgcgctcg aaggcttcaa tctgccgccg
     9361 gaaattgtcg cccccgttgg tcaccaatcg cttatggagg gcggtggttc catccaggtg
     9421 gatttgaacg atgtgttcca agatccggat tatgacgcct tggcttttga agcttcgtcc
     9481 agccggagca atcttgtggc gctcaatttg gccggttcca tgttgacggt gattccgcag
     9541 gcgcgtggcg gagccacggt gaccgtgacg gcggatgacg gggagaatga accggttagc
     9601 agcacgttct atgtgctggt gaatccgacg gcgcacgtct tggcgaatgc cgcgtacacc
     9661 tttggcgagt gggaaaccaa cgaaccggcg ttcagttatc cgacgaatat gctcttttta
     9721 cagagcgcca agaatgatcc cgggctgaat gatgaactac tttacgccta catgattccc
     9781 gcggcggatg cagccttacc ggaagacgcg gagtatccgt atgccgcatc atcgcgcacg
     9841 cgcatcaacg ggctgggaac gaacggcatt gcgttcatca atacggggcg cgggcgcgat
     9901 gtcggcggcg cgctggtcgc gctggatacg cgcggagtgg ctgaagcacc ggtcacctgg
     9961 ctcggcggca cggtgctccc gaacaagcgg atttatgcca ttcgcttgca gtatcgtgtc
    10021 ggcgccacgg gggctttcac cgatgtgctg gacgataatg gccagctggt ggaatacctg
    10081 cgcagcgaaa ccgtggatca cagcgcgaca ctcggcccgg tgtccctgcc cgccgccgca
    10141 ctggaacagg aatatatgca ggtgctgtgg cgctattatt accaaacgga agggtccgga
    10201 gcgcgcgcac aattgcgact ggatgatctg atgattgcca acggaccccc gccgttgccg
    10261 acaggttttg cggcatggca gggggcggag ttctcgcccg aagaattagc gaatcccgcc
    10321 atcagcggtc ctttggccga tccgactgat agtggtttgg ccaatctgat gcgctacgcg
    10381 ctgggattga ctcgtaacgc ggacttccat caggcgctgc cattcgccac cgtgaccggc
    10441 gaaacgcccg ataccgccgc cgtgttccgt catcgccggc tgttgacgcc gaacagcggc
    10501 gtggagtaca ttcctgaata tgctgaaggg ctaatcacgg cgcagtggca caccgcaatc
    10561 accaacagcg acttgatctg gcagggggcc acccccaccg gcgacggcct gaccgaagag
    10621 gtggaatacc tcgtgcccgc cggcagtctc cagcccttgc gcttcttccg tttgaaagtt
    10681 cgactcatgg agtaaaccca gcatgaagcc acgcacggca ctctccggtt ggcggcaatt
    10741 tttctggatc ggttggctgg ccttgggaag cctgccggca tcggcggcaa catggtatgt
    10801 ggccaccaac ggcagcgatg cggccgccgg gacgttggct gcaccttttc ggagcgtcaa
    10861 tcacgccttg cagcaggcgg tgtccggcga cgtggtcgag gtgcgcggcg gcgcgtatca
    10921 agacaccagc gaagtgcgtt tccggcggcc cggcatcacg ctgcgctcct attccaacga
    10981 gtgggcccgg ctcgtggcgc cgctgaataa cgaaaacgac ttctcctcgt gcgtctgggt
    11041 ggatgccgat gccaagggca cggtgctgtc gcgcctggaa atccaaggcg gctattatta
    11101 tggcgtggtg ctgcaaacca aatgggattg gggcgatcca gcggaccgca gcggcgccag
    11161 cggcgtcatc attcaggatt gcgtcattca tgacaccggg cgcgacgcca tcaaaatcac
    11221 gccgaactgc gacgatgtgg tgatcgagcg ctgcgagatt tatcgcagcg gggtggggcc
    11281 ggccaatcag cacgcccaaa atgcggaggg aattgattgc gtcaacagcg accgcctgac
    11341 tgtgcgcgat tgcgtcatcc gcgacgcctt ttccaccggt atttatttga agggcggcgc
    11401 cactgatggg ttgatcgaac gcacccgggt ggagcgctgc ggcgcaggcg gtattttgct
    11461 gggctttgac accagccccg agtttttcga cgtggaggct aatccaagct attacgaaaa
    11521 catccgcggt acggttcgta actgtctggt gcgcgacacc gcctgggagg gcatcggaat
    11581 ctatgcggca tccaacccgg tggtgttcaa caacacgctg ctcaatgttt gcaccgatgc
    11641 ggtgcactcc gccatttact tcgggctgag ctatcaggat tgggagccag aggccggtcg
    11701 ccccgccacc gtcctgccgc agatttacaa caatctggtg attcaaccgg ccggtttcga
    11761 cgacgagatg gtggaaattc gctacgccga tgagttgggc gggttgagtg cgctgcaagg
    11821 ctggccggcc atggattata acctgtattt tgtggccggt ggcggccagc cgtggttcac
    11881 cgatcgccgc ccaggtacgg aactggagcg cggcacgttg gcgcaatggc gggcgcattc
    11941 cggcgctgat gcgcatagcc gcgttgccaa cccgctggcc ttgggcgggg aacatccgct
    12001 gctgctgctc ggtagccccg gcctcaacgc cggcaccaat gcagcgtgga tggcgggcgt
    12061 gacggatttg gtcggccagc cgcgcatctt gggtgggcag gtggatattg gcgcttatga
    12121 aaccggcacg aatgactggg cgcagctcat catcggccat gctgcgctgg ccgccgccac
    12181 gaacccgccg ccggcggccg ccgcgcgcgt ggggcagttg cggtggtttt tcacccatgc
    12241 ctcggtgggc ggcaatctga ttacggggct gaacgggttg tatcaggaaa acgccgcgcg
    12301 ctatccgttg cagatgtata actacgacgg ccaaaatggc gatggcgatt accatggcgc
    12361 ggtgccgact gcaggcacat ctgacgcgcc cgattaccgc gccgccgcgg tcccgtccgt
    12421 caccgccaac gggcggatct atgaatgcca gcgcggcaat ccctcgtggg aaaacaagtt
    12481 ggtttgcttc agcaattctg tggtgcaatc cggctggcgc ttcccgaagg tcaatgtggc
    12541 gctagacaag ttttgctgga ttgatcccgc agccgatcct gccgcctatt gcgccacgat
    12601 ggccggcctt gccgcgcgct atccgcaaac cctgctggtg tatatgacca tgccgctgac
    12661 caccgagact gccggcagcg aaaatgacgc gcgcaacgat ttcaaccgct atgtgcgcgc
    12721 attttgtcgc accaccggcg cgtggctgct cgatctggcg gatatcaccg cgtggaccgc
    12781 ggcgggcagc gagcaaacgt atgtggccgg cggcgtcacg aaccagcggc tggtggccgc
    12841 ctatgctgtg gatgccggca gcggggattt tcatttgaat gcaacgggtc gccgtcgcgg
    12901 cgcgctcggc tggcatgcgc tggcccaagc ccttttccaa accgatcgcg atggcgacgg
    12961 cgtcaccgat ggcgatgaac tgattgccgg caccgtgccg accgatcccg acagcttttt
    13021 ggcccttgtg caggataccg gcggcgcagg cgaaagagtg ctgcgctggg cgggggtgac
    13081 caaccggtgg tacaacgtgc aggaatccac tgcgctgggg accaattccg cgtgggtcac
    13141 gcgcgccgcg catctccccg ccgccgccat caacctgcac acggtggccg tcactgaggc
    13201 atcttcattt tggcgcctta cggtcgaaca atagcgtcaa tccggcatct tttttgcggc
    13261 ggaaagtcgc ggttggaaat gcggcaatgc aaaggccggc tccagcgggg a
//
LOCUS       DAOPNP010000067        13041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5565_length_13041_cov_9.687895, whole genome shotgun sequence.
ACCESSION   DAOPNP010000067 DAOPNP010000000
VERSION     DAOPNP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5565_length_13041_cov_9.687895"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1057)
                     /locus_tag="PKI64_07175"
     CDS             complement(113..1057)
                     /locus_tag="PKI64_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE00826.1"
                     /translation="MKSPAPSHSFSWAIAAMLLALFFWAVVPMMLVHFTQWMDPWTAN
                     GTRYFFAASFWLPFVVRALRCMTPAARRTAWRAAVIPAAAHGTAQVFFGIAPYFNNAT
                     LLNFGCRLSIPFATIFGFWLLKSERALARSPLFWAGLLCALGGFLLMFAQGLGESRAS
                     LLGMLMLLGFSVTWGLYVVFVRRNLTAYPAHLAYGMVSVLTGIPLVGLMFAFGQWAEL
                     GHLTLPQWGWLVLSSLLGLTLGHVLYYRAIRVMGPIASEGFMLLIPFQTAVLAHFLMG
                     DQLTPTQWLAGAVLILGCVLLLRARFQQDRRTTVPAAN"
     gene            1486..2436
                     /gene="fmt"
                     /locus_tag="PKI64_07180"
     CDS             1486..2436
                     /gene="fmt"
                     /locus_tag="PKI64_07180"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOE00827.1"
                     /translation="MEKLRIILLGAGTLACPLLDSILAAGRDELLAVVTQPDRAGGRG
                     LQCQACALKTVALARGLPVLTPADASAPDTVAMLAAWQPDVLIVASFGQFLKKNLLAV
                     PRLGTINVHPSLLPKYRGASPIQWALANGDEQTGVSVLYVTPRMDAGDVLAQAPHPIA
                     PEDTFCTLEPKLAALGAQLLQEVLDGFRAGRTQGVPQDEAQVTLARKLTKDDGRVDWS
                     LPAATIRNRLRGFMPWPGAFTVLPGGLLLKLHGVQVEPWAGGTASPGTVLAVDGAGPL
                     VATGRDALRLTHVQPAGKKAMAGAAFLRGHALPPGSRLGQ"
     gene            2510..3139
                     /locus_tag="PKI64_07185"
     CDS             2510..3139
                     /locus_tag="PKI64_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00828.1"
                     /translation="MDVSLLKELIGYAASLIILVSLLMTNVFRLRLINGVGSVLFAAY
                     GWLIGSWPVCLINLVIAGIDAWYLLEILTSSAYFELAPASSVGPEYLKRIFLFHEREL
                     QKYAPGLTLEDLQEACTCLIFRNMLPVGVFSIRQEGEDAHIIIDFLIAEYRDFKAGRF
                     LYRTKRMFFKERGIKRFHAVARHPSQPKYYLKNGFTRNPDAAGEFLLNL"
     assembly_gap    3204..3303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3304..3987
                     /locus_tag="PKI64_07190"
     CDS             <3304..3987
                     /locus_tag="PKI64_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00829.1"
                     /translation="RRIQMVFQDPQAALNPRLTICDLLTEGLVEHRLLRGESREAAAT
                     RLLAEVGLDESALWKYPFEFSGGQRQRICIARALALEPDVVVCDEVVSALDVSVQAQV
                     INLLVDLRRRHGLAYLMIGHDLSVLRHVAQRLAVMYLGRLAEIGPADSVVGAPQHPYT
                     QASVAAVPVPGAKRERRVLKGEPPSAAKPPAGCAFHPRCPRAMPICRTTPPPACAVGA
                     HTVWCHLYA"
     gene            4092..5063
                     /gene="rfaD"
                     /locus_tag="PKI64_07195"
     CDS             4092..5063
                     /gene="rfaD"
                     /locus_tag="PKI64_07195"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOE00830.1"
                     /translation="MDRKTWTIVTGGAGFIGRNVVAELNRRGVERILVVDDLGHDARW
                     KNLRGLQFEEIWSIAQFREAVAADRVPPTQVVYHLGACSATTETDADYLLDNNFRYTR
                     ELAEWCLRGKARLVYASSAATYGNGEQGYSDADEVTPTLLPLNMYGMSKHLFDLWALR
                     TGALRQMAGLKYFNVYGPGEDHKGDMRSLVNKAYGQVRERGSIRLFKSYRPDYEHGEQ
                     VRDFVYVKDAVDVTLFCGEHPEVAGLFNCGTGEARSWNALAKAVFAAMGLEPNIEYID
                     MPPELRGMYQYFTRAEMGKLRAAGYAAPFTALEDGVRDYVQGYLAKD"
     gene            5101..5913
                     /gene="kdsA"
                     /locus_tag="PKI64_07200"
     CDS             5101..5913
                     /gene="kdsA"
                     /locus_tag="PKI64_07200"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOE00831.1"
                     /translation="MRAVKAGRVKFGGGAPLALIAGPCVLENRAMAFDLAARLVALAR
                     KENIPLVFKASYDKANRTSGQAFRGPGLKEGLAILAAIKEKHGVPILVDVHQVSEVQP
                     AAEVADILQLPAFLCRQTDLVVALGESGRVVNIKKGQFLAPWDIRHVIEKVVATGNRN
                     ILLTERGVSFGYNNLVADMRSLLIMRQFGYPVVFDATHSVQLPGGRAEGTGGDGQWAP
                     YLSRAAVATGCDALFWETHRNPAEAKSDRDNALPLALLGPLWRQCRRLDELA"
     gene            5900..6355
                     /locus_tag="PKI64_07205"
     CDS             5900..6355
                     /locus_tag="PKI64_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00832.1"
                     /translation="MNWRSMGLALALLLGAVGAAAGQIEGSAVTGFRLPEYDEDGNLT
                     QLLYGETATFLPDNIVELTGLKIELYRQGAVYLRAAASQCALDSARGRAASRQHIRIV
                     TDKSVLTGDGFAWNRENQQFQIFQNVKVTLDAQMDKGDLLKPQPAAKKE"
     gene            6358..6882
                     /locus_tag="PKI64_07210"
     CDS             6358..6882
                     /locus_tag="PKI64_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOE00833.1"
                     /translation="MTKLPIGLVAALAVAWLAGSAPRAAAALGDDGQSLEASPNATVI
                     TADKLTFDYLQKFALFENNVLVNDPRLQLSANRLTITLTEDGGVQTIKAEGKVLLTQG
                     DKKARSDMATYDVASGKIVLAGGPPQVMQGRNILEGEVITFWRDEQRLECKPKARLVV
                     YSDDFGNADAYFKK"
     gene            6945..7688
                     /gene="lptB"
                     /locus_tag="PKI64_07215"
     CDS             6945..7688
                     /gene="lptB"
                     /locus_tag="PKI64_07215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOE00834.1"
                     /translation="MTAPDRENYLVETRGLVKEYRRRRVVNGVDMNVRPGEIVGLLGP
                     NGAGKTTTFYMITGLIRPTRGQVHFKGRDVTRLPMYRRARMGLGYLAQEPSIFRNLTV
                     EENVLAILETLPLGARERRRRLEFLLAELKISYLAKQKAYTLSGGERRRLEITRALVS
                     DPALILLDEPFSGVDPLAVYDVQQIIRDLKQRGLGILITDHNVRETLSVVDRAYVIYS
                     GEVLTEGTRDFLVNDPQAREKYLGREFSM"
     gene            7742..8083
                     /gene="raiA"
                     /locus_tag="PKI64_07220"
     CDS             7742..8083
                     /gene="raiA"
                     /locus_tag="PKI64_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOE00835.1"
                     /translation="MNIQITGRNVTVNAGLKEYVEGRLGPVLAGYPRVESAHAILTME
                     KYRFTAEFVVQGKDKLREVAGDTTNDMYAAIDSAAVKLDKQLRKSRQKVVQKHKSRQG
                     RTRLADVEPTE"
     gene            8142..9095
                     /gene="hprK"
                     /locus_tag="PKI64_07225"
     CDS             8142..9095
                     /gene="hprK"
                     /locus_tag="PKI64_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOE00836.1"
                     /translation="MWRVANSPRITVADFFAAGRAPLQLVWEANPEAAVGRIITEVAL
                     NRPGLALAGFRRYFANRRLQVFGLAEMAYLGSLPPEERSQRLRALGRVPALVMSRGRR
                     LPAYAREVVEHLKIPVMRTQLVTGHFMNNATVLLQNMTSPRIRVAGTMVEINGVGVLL
                     EGEPGIGKSEIALALIERGHSLVSDDTTVLMRDSTGTIQGSAVEITRHHMEIRGLGII
                     HVPSLFGIASMRNERRLDLIICMRKAENVGGDIDRTGLETASRNVLGVEIPLITVPVA
                     PGRDLTNVVEVAALNQRMKQMGRDAARELDEKLKVALARKD"
     gene            9100..9402
                     /locus_tag="PKI64_07230"
     CDS             9100..9402
                     /locus_tag="PKI64_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE00837.1"
                     /translation="MTPNPSSSAAPVTLTQEVVVQNRYGIHARPAALLVKTATQFACE
                     IMLEKNGVKVNAKSIMGLLTLEGNHGAKMKIHATGADAAAAVAALVELFARKFHED"
     gene            9416..11185
                     /gene="ptsP"
                     /locus_tag="PKI64_07235"
     CDS             9416..11185
                     /gene="ptsP"
                     /locus_tag="PKI64_07235"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE00838.1"
                     /translation="MAAQPELELSGIGVAPGIVFGPALVLPADDVAPPDFAIPPEAVE
                     HETLRFERALLATRRQLNDLQTRVSLAMGTESARIFEAHLHVVDDPSFVDEIYHDLRA
                     ELRNIESITYRVAERYAQALQQMEDDYLRERATDIRDVTRRILRNLGGGGSELVGSFD
                     APRVLIARDISPSELAAYDRSKLLGMVTELGSLTAHTAILARAMDLPAIVGLRGATAR
                     VPEGSAVILDGARGVLVVNPTAERTAEYQRLARGQQAIQQRLATLKDELAVTRDGHLV
                     ELSANIELPADADMALARGARGIGLFRSEYLFLAGNGEPTEDAQYQAYADVARKIHPE
                     PVIIRTLDLGGDKLLADGRAHTEANPFLGWRAIRLCLDRPDFFKTQLRAILRASAHNP
                     RVRIMYPMISSAAEVTQANALLAECKQDLAAAGVPFNAAIETGVMIEIPAAALTAELI
                     APHVQFFSIGTNDLVQYTLAVDRGNERVASLYDPAHPAVLQLIQRTVAAGQRHGIWTG
                     VCGEAASNPLFVPLLLGLGVTELSVGPGAVPLVKDVIRHIHLAAAQDLAREALALADG
                     AAIRSRCRDFVRECAPEILELAG"
     gene            11235..12428
                     /gene="metK"
                     /locus_tag="PKI64_07240"
     CDS             11235..12428
                     /gene="metK"
                     /locus_tag="PKI64_07240"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOE00839.1"
                     /translation="MSKNTVFTSESVSEGHPDKLCDAVSDAILDACLAQDPRSRVACE
                     TLAKTGMVVVAGEITTKAVVNYAEVARKAVNEIGYNHQEAGFNGNICAVLVAIENQSP
                     DIAQGVNAAKATGKATAEQGAGDQGMMFGYACDETPELMPLPIVLAHRIMRKLTDLRK
                     AGKMPWVRPDSKSQVSVEYDANGNPVRVDTIVVSTQHAPHVTHQQIRKEIIEKVIKTS
                     VPAKLLDKRTVYHVNPTGRFVVGGPQGDCGVTGRKIIVDSYGGMGRHGGGAFSGKDPS
                     KVDRSAAYMARYVAKNIVAAKLAKRCEVQLAYAIGYPEPVSVHVDTFGTGTVADDKIA
                     KAVRQVFGLKPAEIIKQLDLLRPIYSLTNQYGHFGRKKGDKNFKLFTWEQTDKASALV
                     KALAK"
BASE COUNT         2441 a         4006 c         4241 g         2253 t
ORIGIN      
        1 catcgtgctg attgcggtga cgctgctggc gctggcgggc tacattatgc accaccgagt
       61 gcggatgccc agccggccgg gggccgttga atttatcgag ccgacgcctt gatcagtttg
      121 cggcggggac ggttgtgcgc cggtcttgct ggaagcgtgc gcgcaacagc aggacgcatc
      181 cgagaatcaa caccgcgccg gccagccatt gggtgggggt gagttggtcg cccatcagga
      241 aatgggccag cacggcggtc tggaagggga tcagcagcat aaagccttcc gaggcgatgg
      301 gacccatcac acggatggcg cggtaataca gcacatggcc aagcgtgagc cccagcagcg
      361 acgacaacac cagccacccc cactgcggca gcgtcaagtg gcccagctcc gcccactggc
      421 caaaggcgaa catgagaccc accagtggaa tgccggtcaa cacgctgacc atgccgtagg
      481 ccagatgcgc gggataggcc gtgagattgc gccgcacaaa caccacgtag agtccccacg
      541 tcaccgaaaa acccagcagc atcagcatgc ccagcaggga ggcgcggctt tcgccgagcc
      601 cttgggcaaa catcagcaag aagccaccca gcgcgcagag cagacccgcc cagaacagcg
      661 gggagcgggc cagcgcgcgt tcgcttttca gcagccagaa gccgaaaatg gtggcgaatg
      721 ggatggagag ccgacagccg aagttgagca gcgtggcatt gttgaagtag ggcgcgatgc
      781 caaagaaaac ctgcgcggtg ccgtgcgccg ctgcgggaat cacggcggcg cgccaggcgg
      841 tgcgccgcgc ggcgggcgtc atgcagcgca gcgcgcgcac cacaaatggc agccagaacg
      901 atgccgcaaa aaagtagcgc gtgccattcg ccgtccacgg gtccatccat tgcgtaaaat
      961 gcacgagcat catgggcacc acggcccaga aaaacagggc cagcagcatg gcggcaatgg
     1021 cccaagagaa tgaatgggat ggcgcggggg acttcatcgg gcaggggagt gtacgccctc
     1081 gcgcccggaa gcaaagcaaa atgcgcccgc cggttttttg cgctgcgccg gacaggggcg
     1141 gggggttcta gcctgagttc cgcgcactcc gaaaatctcg ttgtcacgcg ttgcattgcc
     1201 agcggacgat ttcgttttgg ggtgccccgg tcccaaaacc gcttccggtc ggattgtaag
     1261 gctgaaaagg tcgcagacct tccagccgtt cccggcggga aggtttcgtt cctcaaccat
     1321 tcccgcccta caaaagccct gcaaaaaaac tgcgaaattg ttgggtgtcc caatgctgaa
     1381 ctcagcaaaa tgcgcccgcg gtaatttctg cgctgcgccg cgcgggggca ttgggggtgg
     1441 gttttgcttg ccgcgcgggg gtggggggtc tagcgtaggg gccgtatgga aaagttgcgg
     1501 atcattttgc tcggtgcggg cacgttggct tgcccgttgc tggattccat cctggcggcg
     1561 ggtcgcgatg aactgctggc agtggtgacg cagccggacc gcgcgggcgg tcgcggattg
     1621 caatgccaag cctgtgcgct gaaaacggtg gcgttggcgc gcggcttgcc ggtcttgacg
     1681 cccgcggacg ccagcgcgcc ggatacggtg gccatgctgg ccgcatggca gcccgacgtg
     1741 ctgattgtgg cgagcttcgg ccaattcctg aaaaagaatc tgctggcggt gccgcgtttg
     1801 gggacgatca atgtgcatcc ctcgctgctg ccgaagtacc gcggggccag ccccatccaa
     1861 tgggcgctgg cgaatggcga cgagcaaacc ggggtcagcg tgctttatgt gacgccgcgg
     1921 atggatgcgg gcgacgtgct ggcgcaagcg ccgcacccca tcgcgccgga ggacacgttc
     1981 tgcacgctgg agccgaagct ggcggcgctg ggcgcgcaac tgctgcaaga ggtgttggac
     2041 ggtttccgcg cggggcggac gcagggagta ccccaagacg aagcgcaagt gacgctggca
     2101 cgcaaattga cgaaggacga cgggcgcgtg gattggtcgc tgccggcggc gaccatccgg
     2161 aaccggctgc gcgggtttat gccgtggccg ggggcgttca ccgtgctacc cggcggcttg
     2221 ttgctcaagc tgcacggggt tcaggtggag ccatgggcgg gcggaaccgc ctcacccggc
     2281 accgtgctgg cggtggatgg cgcggggcca ctggtggcca ccggccgcga cgccctgcgc
     2341 ctgacccacg tgcagccagc gggcaaaaag gccatggcgg gcgcggcgtt tttgcgcggg
     2401 cacgcgctgc cgccgggttc ccggctgggg caatgacttt gacgcgtcgc cgggaggcgg
     2461 ctatagtggg gctgccgctg ggtacccgcc cggcgcaaga gagggtagca tggatgtttc
     2521 gttgttgaag gagctcatcg gttacgcggc gtcgctgatc attttggtct cgctgctgat
     2581 gaccaatgtg tttcgcctgc ggttgatcaa tggcgtcggc tcggtgttgt ttgcggccta
     2641 cggttggctg atcggctcgt ggccggtgtg cctcatcaat ctggtgattg ccggcattga
     2701 cgcgtggtac ttgctggaga ttctgaccag cagcgcttac tttgagttgg ccccagcctc
     2761 cagcgtgggg ccggaatatc tcaagcgtat tttcctgttc cacgagcggg aattgcaaaa
     2821 gtacgcgccg ggactcacgc tggaagattt gcaggaagcc tgcacgtgcc tgatcttccg
     2881 caacatgctg ccggtgggtg tgttttccat ccggcaagag ggcgaggatg cgcacatcat
     2941 cattgatttt ctgatcgcgg aatatcgcga cttcaaagcc gggcgttttc tgtaccgcac
     3001 caagcggatg tttttcaagg agcggggcat caagcggttt cacgcggtgg cgcggcatcc
     3061 gtcgcagccg aaatattatt tgaaaaatgg cttcacgcgt aacccggacg cggccggtga
     3121 atttttgctg aacctgtgag ttccgttccg gcaacgccct ggctggagat ggccgatgtg
     3181 gcggtacggt ttccgatccg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnncggcgca tccagatggt ttttcaggat ccacaggcgg cgctgaatcc gcgcctgacc
     3361 atctgcgacc tgctgacgga aggcctcgtg gagcatcggt tgctgcgcgg cgagagccgc
     3421 gaggcggctg ctacccgctt gttggcagag gtggggctgg atgaaagtgc gctctggaaa
     3481 tatccgttcg agttttccgg cgggcagcgg cagcgaattt gcatcgcgcg ggcgctggcg
     3541 ctggagccgg atgtggtggt gtgcgacgaa gtggtcagcg cattggatgt cagcgtgcag
     3601 gcgcaggtga tcaacctgct ggtggatttg cgccggcggc acggcttggc ctatctgatg
     3661 atcggccacg atttgagcgt gctgcggcat gtggcccagc ggctggcggt gatgtatttg
     3721 ggccgcctgg cggaaatcgg cccggcggat tccgtggtgg gcgcgccgca gcatccttat
     3781 acgcaggcat cggtggcagc ggtaccggta cccggagcca aacgcgagcg ccgggtgctg
     3841 aagggcgagc cgccttccgc cgccaagccc ccggccggct gcgcgtttca tccgcggtgc
     3901 ccgcgggcca tgcccatctg ccgcaccacg ccgccgccag cctgcgccgt gggcgcgcac
     3961 accgtttggt gccatctcta tgcctgatgc ccgccgggca ggctgcgtct gcgccccctc
     4021 cgcggtttag aaaatgcggc ttgctggtgc gggcgggggg gtgctaggtt gccggtttga
     4081 aaggccagtc aatggatcgc aagacgtgga caattgtgac gggcggcgcg ggattcatcg
     4141 gccgcaacgt ggtggcggaa ctgaatcggc gcggggtgga gcggattctg gtggtggacg
     4201 atttgggcca tgacgcgcgc tggaagaact tgcgggggtt gcagttcgag gaaatctggt
     4261 ccatcgccca atttcgcgag gcggtggcgg cagaccgtgt gccgcccacg caggtggtct
     4321 atcatctcgg ggcgtgcagc gccaccaccg agaccgatgc ggattatttg ttggacaaca
     4381 acttccgcta cacgcgcgaa ttggccgagt ggtgcctgcg cggcaaggcg cgcttggtct
     4441 atgcctccag cgcggccacc tatggcaacg gcgaacaggg ttattcggac gcggacgaag
     4501 tcacgcccac cctgctgccg ctcaacatgt atggcatgag caaacacctg tttgatttgt
     4561 gggcgctgcg caccggcgcg ctgcgccaga tggcgggcct caagtatttc aatgtgtacg
     4621 gccccggcga ggatcacaag ggcgacatgc gttcgctggt gaacaaggct tatggccagg
     4681 tgcgcgaacg cggctcgatc cggctcttca aatcgtaccg ccccgattat gaacacggcg
     4741 agcaggtgcg cgatttcgtc tatgtaaagg atgccgtgga tgtgacgctc ttctgtggcg
     4801 aacatccgga ggtggccggg ctgttcaact gtggcactgg cgaggcgcgc tcgtggaacg
     4861 cgctggccaa ggcggtgttt gccgccatgg gcctcgaacc gaacatcgaa tatattgaca
     4921 tgccgccgga attgcgcggg atgtaccagt attttacgcg ggccgagatg ggcaagctgc
     4981 gggcggcggg ctatgccgcg ccgttcactg cgctggaaga tggcgtccgc gactatgtgc
     5041 agggctattt ggccaaggat tgaggcgcgc gtgagcaaga atccatttcc caaagcgccg
     5101 gtgcgggcgg tcaaggcggg ccgggtgaag tttggcggcg gcgcgccgct ggccctgatt
     5161 gccggtccct gcgtgttgga aaaccgcgca atggcttttg atttggcggc gcgcctcgtg
     5221 gcgctcgcgc gcaaggaaaa catcccgctg gtgttcaagg cttcctacga caaggccaac
     5281 cgcaccagcg ggcaggcgtt ccgtgggccg ggcctcaagg agggcctcgc cattctggcc
     5341 gccatcaagg aaaaacacgg cgtgccgatt ctggtggatg tgcatcaagt cagcgaagtc
     5401 caacctgccg cggaagtggc cgatattctg caattgcccg ccttcctctg ccgccagacg
     5461 gatttggtgg tggcgctggg cgagagcggg cgggtggtga atatcaagaa ggggcaattc
     5521 ctcgcgccgt gggatattcg ccacgtcatt gaaaaagtgg tcgccacggg caaccgcaac
     5581 attttgctga cggagcgcgg ggtgagcttc ggctacaata atctggtggc cgatatgcgc
     5641 agcctgctga tcatgcgcca gttcggctac ccggtggtat tcgatgccac gcacagcgta
     5701 caactgcccg gcgggcgcgc ggagggcacc ggcggcgacg gccagtgggc gccctatttg
     5761 tcgcgcgcgg cagtggccac cggttgtgac gccctgttct gggaaaccca ccgcaacccg
     5821 gccgaggcca agtcggaccg cgacaacgcg ctcccgctgg cgctgttggg gccgctgtgg
     5881 cggcaatgca ggaggctgga tgaactggcg tagcatgggc ttggcgctgg cgctcctgct
     5941 gggggcggtg ggggcggccg ccggccagat cgagggctcg gcggtgaccg ggttccgcct
     6001 gccggaatat gacgaggacg gcaacctgac gcaactgctc tacggcgaaa ccgccacgtt
     6061 tctgccggac aacatcgtgg agctgacggg gctcaaaatc gagctgtacc ggcagggggc
     6121 ggtctatctg cgcgcggccg cgtcccagtg cgcgctggat tcggcgcggg gccgcgcggc
     6181 ttcgcgccag catatccgca tcgtgaccga taaatccgtg ctgaccggcg acggcttcgc
     6241 gtggaaccgc gagaatcaac agttccagat tttccagaat gtgaaggtga cgctggatgc
     6301 gcagatggac aagggcgatt tgctcaagcc gcagccggcg gcgaaaaagg agtgagtatg
     6361 acgaagttgc caattgggct ggtggcggcg ctggccgtcg cgtggctggc ggggagcgcg
     6421 ccgcgggccg ccgccgcgct gggggatgac gggcagtcgc tggaggcttc gcccaacgcc
     6481 acggtgatca ccgccgacaa gctcacgttc gactacctgc aaaaattcgc gctgtttgaa
     6541 aacaacgtgc tggtgaacga cccgcgcctg caactgagcg cgaaccgcct gacgatcacc
     6601 ctcaccgagg atggtggcgt gcaaaccatc aaggccgaag gcaaagtgct gctgacccag
     6661 ggcgacaaaa aagcgcgctc cgacatggcc acctacgacg tggcctccgg caagatcgtg
     6721 ctggccggcg gcccgccgca ggtcatgcag ggccgcaaca tcctcgaagg cgaggtcatc
     6781 acgttctggc gcgatgaaca gcgcctcgaa tgcaagccca aggcgcggct ggtggtctac
     6841 tcggacgact tcggcaatgc ggatgcctac ttcaagaaat agggcgcaaa agacttgtcg
     6901 ggccatcgcc gccaaggtaa agtgtccttg atgaggtatt actggtgacc gcaccggatc
     6961 gagaaaacta tctcgtcgaa acccgtgggc tggtgaagga ataccgtcgc cggcgcgtgg
     7021 tgaacggcgt ggatatgaat gtgcgcccgg gggaaattgt ggggttgctc ggtcccaatg
     7081 gcgcgggcaa aaccaccacg ttttacatga ttacggggct gattcggccc actcgcggcc
     7141 aagtccactt caagggccgg gatgtgacgc gcctgccgat gtatcgccgg gcccgcatgg
     7201 ggctggggta tctggcgcag gagccgagca tcttccgcaa cctgaccgtg gaagaaaacg
     7261 tgctggccat tctcgaaacc ctgccgctgg gcgcgcgcga gcggcggcgg cgcctcgaat
     7321 tcctgctggc ggagttgaag atcagctacc tcgccaagca aaaggcctac acgctcagcg
     7381 gcggcgagcg ccgccggctc gaaatcaccc gcgcgctggt gtcggatccc gcgttgatac
     7441 tgctggatga acccttcagc ggcgtggacc cgctggcggt ctatgacgtg cagcaaatca
     7501 tccgcgacct caagcagcgc ggtctgggta ttctcatcac cgaccacaac gtgcgcgaga
     7561 cactctccgt ggtggaccgc gcctatgtga tttactccgg cgaggtgctc accgagggca
     7621 ctcgcgattt cctggtgaac gatccgcagg cgcgggagaa atatttgggc cgggagttca
     7681 gcatgtgagc cggccccgtg gagcagcaaa caacacaacc aaaccaaaaa ggacaaccgg
     7741 aatgaacatc cagatcacag gacggaacgt aacggtaaat gccggcttga aggagtatgt
     7801 ggaaggacgg ctggggccgg tcttggccgg ctacccgcgc gtggaaagcg cgcatgcgat
     7861 tctcacgatg gaaaaatacc ggtttaccgc ggagttcgtg gtgcagggca aggacaagct
     7921 gcgcgaagtg gccggcgaca ccaccaacga catgtatgcg gccattgatt ccgccgccgt
     7981 caaattggac aagcaattgc gcaagtcgcg ccagaaagta gtgcaaaaac acaagagccg
     8041 ccagggtcgg acccggctgg cggatgtgga gccaacggag taggacagca gccagcaaac
     8101 cggggccgac ccgccgccgg ccccgcccgg aaaacaacgc catgtggaga gtagccaata
     8161 gcccgcggat cacggtggcc gacttttttg cggccggccg cgcgccgttg caattggtgt
     8221 gggaggccaa tcccgaggcg gcggtcgggc gcatcattac cgaggtggcg ctcaatcgtc
     8281 cggggttggc gctggcgggc ttccgacgct attttgccaa ccgccgcttg caggtgtttg
     8341 gcctcgcgga gatggcttat ctcggcagtt tgccccccga agagcgcagc cagcggctgc
     8401 gcgcgctggg gcgcgtgccg gcgctggtga tgtcgcgcgg gcggcgcttg ccggcctatg
     8461 cgcgcgaagt ggtcgagcac ctgaaaatcc cggtgatgcg cacccaactg gtcaccggcc
     8521 acttcatgaa caacgccacg gtgctgttgc aaaacatgac cagcccgcgc atccgcgtgg
     8581 ccggcaccat ggtggaaatc aacggcgtgg gcgtgctgct ggaaggcgag ccgggcatcg
     8641 gcaaaagcga aattgcgctc gcgctgatcg agcgcggcca cagtcttgtg tccgacgaca
     8701 ccaccgtgct catgcgcgac agcaccggca ccattcaggg ttctgcggtg gagatcacgc
     8761 gccaccacat ggaaatccgc ggcctcggca tcatccatgt gcccagcctc tttggcattg
     8821 cctccatgcg caacgaacgc cgcctcgatt tgatcatttg catgcgcaag gccgagaatg
     8881 tcggcggcga tattgaccgc accggcctcg aaaccgccag ccgcaacgtg ctcggcgtgg
     8941 agatcccttt gattaccgtg cctgtggcgc ccggccgcga cctgaccaac gtggtcgaag
     9001 tggccgcgct caaccagcgc atgaaacaaa tgggccgcga tgccgcccgt gaattggacg
     9061 aaaaactgaa agtggccttg gcccggaaag attgatcccg tgacgcccaa cccctcctca
     9121 tccgccgccc ccgtcaccct cacccaggag gttgtggtac aaaaccgcta cggcatccat
     9181 gcgcggcccg ccgcgctgtt ggtcaagacc gccacccagt tcgcctgcga aatcatgctc
     9241 gaaaaaaacg gggtgaaggt gaacgccaag agcatcatgg gccttttgac gctggaaggg
     9301 aaccacggcg ccaaaatgaa aatccacgcc acgggcgccg atgccgccgc cgccgtggcg
     9361 gcgctggtgg agttgttcgc ccggaagttc cacgaggatt gagggggccg gcagtatggc
     9421 ggcgcagccg gaactcgaac tcagcgggat cggcgttgcg cccggcattg tgttcggccc
     9481 ggccctcgtg ttgccggcgg atgatgttgc cccgcctgac ttcgccattc ctcccgaggc
     9541 cgtcgaacac gaaaccctgc gctttgagcg cgcgctgctg gccacccgcc gccagctcaa
     9601 cgacctgcaa acgcgcgtca gcctcgccat gggcaccgaa agcgcacgaa tttttgaagc
     9661 ccacctgcac gtggtggacg accccagctt cgtggatgaa atctaccacg atttgcgcgc
     9721 tgagctgcgc aacatcgaga gcatcaccta ccgcgtggcc gaacgctatg cgcaggcgct
     9781 ccagcaaatg gaagacgact atttgcgcga gcgcgccacc gacatccgtg atgtcacccg
     9841 ccgtattctg cgcaacctgg gcggcggcgg cagcgagctg gtcggcagct tcgacgcgcc
     9901 ccgcgtgctg atcgcgcggg atatttcacc ctccgaattg gccgcctacg accgcagcaa
     9961 gctgcttggc atggtgaccg agctgggcag cctcaccgcg cacaccgcca tcttggcgcg
    10021 agccatggac ctgccggcca tcgtgggcct gcgtggcgcc accgcccgcg tgcccgaagg
    10081 cagcgccgtt attctcgatg gcgcgcgcgg cgtgctggtg gtcaacccca ccgccgaacg
    10141 cacggcggaa tatcagcgcc tcgcgcgcgg gcagcaggcc atccagcagc gcctcgccac
    10201 gctgaaagat gaactcgccg tcacccgcga tggccacttg gtggagctct ccgccaacat
    10261 cgaactgcct gccgatgccg atatggcgct ggcccgcggc gcgcgcggca tcggcctgtt
    10321 ccgcagcgaa tacctgtttt tggccggcaa cggcgagccg accgaagacg cgcaatatca
    10381 ggcctatgcc gatgtcgcgc gcaaaatcca tcccgaaccg gtcatcatcc gcacgctgga
    10441 tctggggggc gataaactcc tcgccgatgg ccgcgcgcac acggaagcca atccgttcct
    10501 cggctggcgc gccatccggc tctgcctcga ccgccccgac ttcttcaaaa cccaactgcg
    10561 ggccattctc cgcgcctccg cgcacaatcc gcgggtgcgc atcatgtatc ccatgatcag
    10621 cagcgcagcg gaggtgacgc aagccaatgc cctgcttgcc gagtgcaaac aggacttggc
    10681 cgctgccggc gtgccgttca atgccgccat cgaaaccggc gtcatgatcg aaatccccgc
    10741 tgccgcgctg accgccgaat tgatcgcgcc ccacgtacaa tttttcagca tcggcaccaa
    10801 tgacttggtg caatatacgc tggcggtgga ccgcggcaac gagcgcgtgg catcgttgta
    10861 cgaccccgcc catcccgccg tgctgcaact catccaacgc accgtggccg ccgggcaacg
    10921 gcacggcatc tggaccggcg tttgcggcga agccgccagc aatccgctgt tcgtgccgct
    10981 gttgctcggg ctgggcgtga cggaattgag cgtgggcccc ggcgccgtgc ctctggtcaa
    11041 ggatgtcatc cgccacatcc atctcgccgc agcgcaagac ctggcccgcg aagcgcttgc
    11101 cttggccgac ggcgccgcca tccggagccg ctgccgcgat tttgtgcgcg aatgcgcgcc
    11161 agaaatactt gaactggccg ggtaatcgag tagattcccc aagtcaactc gtcaaaaaaa
    11221 cgaaggagcc tcccatgtcc aagaatactg tgttcacctc cgaatccgtc tcggaaggac
    11281 atcccgacaa actgtgcgac gccgtctcgg atgccattct ggacgcctgc ctcgcgcaag
    11341 acccgcgcag ccgcgtcgcg tgcgaaacgc tcgccaagac cggcatggtc gtggtcgccg
    11401 gcgaaatcac caccaaggcc gtggtcaact acgccgaagt cgcccgcaag gccgtcaacg
    11461 aaattggcta caaccatcag gaagctggct tcaacggcaa catctgcgcc gtgctcgtgg
    11521 ccattgaaaa ccagtcgccg gatatcgccc agggcgtcaa cgccgccaag gccaccggca
    11581 aagccactgc cgagcagggc gccggcgacc aaggcatgat gttcggctac gcctgcgacg
    11641 aaacccccga gctgatgccg ctgcccatcg tgttggcgca ccgcatcatg cgcaaactga
    11701 ccgatctgcg caaagccggc aagatgccgt gggtccgccc cgactccaag agccaggtgt
    11761 ccgtggaata cgacgccaac ggcaaccccg tgcgggtgga taccatcgtt gtctccacgc
    11821 agcacgcccc ccacgtcacc caccagcaaa tccgcaagga aatcattgag aaggtcatta
    11881 agacctccgt ccccgccaag ctgctcgaca agcgcacggt ctatcacgtc aaccccactg
    11941 ggcggttcgt ggtgggcggt ccgcaaggcg attgcggcgt caccggccgc aagatcatcg
    12001 tggattccta tggcggcatg ggccggcatg gcggcggcgc attctccggc aaggacccct
    12061 ccaaggtgga ccgcagcgcc gcctacatgg cgcgctacgt ggccaagaac atcgtggccg
    12121 ccaaactcgc caagcgctgc gaagtgcaat tggcctacgc catcggctat cccgaaccgg
    12181 tttccgtgca cgtggatacc ttcggcaccg gcaccgtggc cgacgacaag attgccaagg
    12241 ccgtgcggca agtgttcggt ctgaaacctg cggaaatcat taagcagctt gatctcctgc
    12301 gccccatcta ctccctcacc aaccaatacg gccacttcgg ccgcaagaag ggcgacaaaa
    12361 acttcaagct cttcacgtgg gagcagaccg acaaggcttc cgccctcgtc aaggccctcg
    12421 ccaagtaatc cgccccgccg ccgccgcctc aaggcggcgg caaaggcttg atgatttgca
    12481 atagccatgc tcgttgagcg tggggttgtt ggcccgacgg cagtatcggc tgttggctgt
    12541 tcttgtagcc ggcctcggtg aggccggggc tgttctggct gttcgcctgt aacctgcaac
    12601 cttgaacctg caaccctctt ctggtgtttg atcaggagga tttcttgccg attttggcgg
    12661 cgccaaaacc aaccgacgtc ttttccgccc tggcgcgccg cgcccgaccc ctcgcggaca
    12721 cgagtccagc tcgggtcgtc cgcaacgcgc catcatcgga aaatccaccg tttattttgg
    12781 cgcaacagcc ccgccaaaat ccgcaagaaa tcctccgctt gcaggtgctg cgcacagcgg
    12841 acaaggcccg gcaaggcccc tatggaactg cctacttcgc gggacgctcg tcagcagaat
    12901 cagaggtcag agattttttc tgtagccggc ctcactgaac acagggctgt tgcccgtctt
    12961 tcgctattcg gatcaaaaac aggctgtttt tgaggtgcgg accatgattt ggccctttgt
    13021 ttacaagggt tgatgcttca a
//
LOCUS       DAOPNP010000068        12790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5696_length_12790_cov_9.146133, whole genome shotgun sequence.
ACCESSION   DAOPNP010000068 DAOPNP010000000
VERSION     DAOPNP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_12790_cov_9.146133"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..1044)
                     /locus_tag="PKI64_07245"
     CDS             complement(367..1044)
                     /locus_tag="PKI64_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE00840.1"
                     /translation="MPPAVPYSLLLVDDHAIVRQGLAALLTRESDFTIVGEAGTYADG
                     LAAVLALQPAGVVLDLSLRDQSGLDWIREARAKGYTGKILVLSMHDEDLYADKALRAG
                     AQGYVMKDQAEETLVAALRTVLAGGTHLSPAAAALLAGSAESAGKAADGFGALTARES
                     EILASMGSGLTTRDIAEKFGLSGRTVEVHRGNIKRKLGCHSLAEMMVKAAEFRRAQDA
                     ALQPPAL"
     gene            complement(1051..2049)
                     /locus_tag="PKI64_07250"
     CDS             complement(1051..2049)
                     /locus_tag="PKI64_07250"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOE00841.1"
                     /translation="MRVAVLGDGGWGTALALTLVRNGHSPTVWGPNAEYLDTVRQTRA
                     NPKFLPGVPLPESVSWTADPATALAGAEAVMLVVPSQYVRATLTRFAPAYHAAGSPPV
                     VSATKGFAEDTRERISALLTSLWGVAQPAVLSGPSIAPETARGVPTAVTVACADSAVA
                     ARFQELLTGLTFRVYTSDDVCGVEVGGALKNVIAIAAGVCDGLGFGHNAKAALITRGL
                     AEMTRFGVDLGAHPQTFQGLSGIGDLLVTCMSPQSRNRGVGERLGRGETLADILRGME
                     QVAEGVWTSGTALALARQLGVAVPVMEQVDAILHHGKRPKEAVVELMSRDPRAERD"
     gene            complement(2046..3008)
                     /locus_tag="PKI64_07255"
     CDS             complement(2046..3008)
                     /locus_tag="PKI64_07255"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOE00842.1"
                     /translation="MTIRLSPNHRLKPLFEQHHVAWLSEEAAERQDIRPLHIGILNIM
                     PRAEDYEFNLLAPMGRSILQIIPIWIRLRNHSYTSSDQDHLDKNYLPFDWAQSVAALD
                     GLIVTGAPVEELPWEQVTYWPELCGIFDAAMRGGTCLLGICWGGLALAKYLGIEKMKY
                     PQKIFGVYPTRNLVPDHPIMGGLDDVFWCPQSRHSGVADDVLEAAAAAGKVRLLAHAD
                     KGGYTIFETPDHQVIMHLGHQEYNSSRLLAEAQRDRLKARADVGPLENLDEQNPINNW
                     RANRNEFFNSWIKHVYLTTPYDKGADPAKATNEIVVGKQEPPAP"
     gene            complement(3064..4557)
                     /gene="hydG"
                     /locus_tag="PKI64_07260"
     CDS             complement(3064..4557)
                     /gene="hydG"
                     /locus_tag="PKI64_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOE00843.1"
                     /translation="MLAVEQKQWLRERIVPAEIEKYLPADGQDFINDADIERLLVITG
                     EPEAGAVREIIAKALAVQTLSQAELATLMRVRDPELWAEMRTAAGAVKRKVYDNRIVT
                     FAPLYMSSKCVNDCVYCGLRRSNPAVERRVLNMAEVRAETEALAGKLGHKRLIVVYGE
                     HPESGVDYIAESIRTVYSVRVSTRRGMGQIRRVNVNAAPMSVAELKVLHECGLGTFQV
                     FQETYDRRAYARLHPAGTIKSDFRWRLYAMHRAQEAGIDDNGIGALYGLTDWRFELLG
                     QLAHAIEMERFCGIGPHTLSFPRLEPAVNTPFNEQSPWRVSDDDFERIVVLARLAVPY
                     AGLIITARETPAMRRRLLPLGVTQMDAASNIGVGAYADGQEKQKGERQQFLLGDPRGL
                     DEVVREWAHAGHITSFCTAGYRCGRTGKCIMDLLRTGVEGKFCKLNAILTFKEWLDDF
                     ATPETQAVGEALLARELEEVRQQQPRAWPAFWANYQRTVAGERDLYF"
     gene            4768..6282
                     /locus_tag="PKI64_07265"
     CDS             4768..6282
                     /locus_tag="PKI64_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOE00844.1"
                     /translation="MNTPATPQSPLRMVMLITLPIVGILLAIIIALVMRKPHDNARVT
                     VVVTDGQPGLDVNTGAILDATVTDQPVLPRVGYRYKVFVDDESDDRSSGIAKIGGRAT
                     FIPGARRGQTAIVDVTRVRDRAVDAIMVKVLSEVALPPKVLRPAFVPQPGDSAAHVMP
                     GAEMDVVITEASERNPDTEGVAKVSGLVVFVRGATQIGERVNVRITERREKMAFAMLT
                     GKPAGTEPLPAAAPDRPAYVPPPGDPVVVGAEMDVQITEASEKNPDTEGVARIGGLVV
                     FVKGVTAIGERVNVRIVERRPKSAFAEPTGKPAGAAPLASQGTTGDILRRAFTPKAGD
                     PAGHVIAGAEMDVVITEASQQRPGQEGVAKVAGLVVFVDGATTIGERVNVRITARRER
                     MAFAELTGKPAGTEPLPAAAPSAPPPPPRPYVPPADDATAFIVPGAVISATISEASEK
                     NPETEGVARVAGLVVSVKGATTIGETVNVRITERRRSMALGEKTTDAVTAHPAD"
     gene            6318..7094
                     /locus_tag="PKI64_07270"
     CDS             6318..7094
                     /locus_tag="PKI64_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOE00845.1"
                     /translation="MAQTLTNETNLRTRQAAIERELTWLKRIRVIEWGGAILFALIGL
                     ALRQMGIAAANFVLFIAGVLVFLALGHEARRREQISDLADTADLRGVKEKFAQLLDEK
                     LANDHFVLNDLHLIVGREKCWIDHLVVAPSGMFVIDILNWDGTIVGDTGKQVHTWKIR
                     TPDGKVRPARNPVSRTLRGRRILCNWLKGTNLIWERVFPLVVLANPDADIQVTNAARR
                     VLTPEQAVTFINDFCFEKPVLSNAEVEKLARGLNQHTTGA"
     gene            7111..7866
                     /locus_tag="PKI64_07275"
     CDS             7111..7866
                     /locus_tag="PKI64_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOE00846.1"
                     /translation="MAKILRNESSLRRRQDAIRRELEFRQITRIIAWSIVLAIVVWGL
                     LTTIRGGSFAGVVVAGVVIMLGAGYEMRLREIEKEIRNIEGGRRGEQKMAERLAEQLA
                     DDHVILNDLDLRIAHERGQIDHLIIAPSGIYVMESKYWAGILSGEADAATWTQRRTNG
                     LTRRVKSPVQQCERQRRMFITLLAKRVPDDHVHAMAVFTHPSVELHIANGENRAFLIR
                     DAIRFINDRCFEPPVLTPEQVQEIAESVLRQQT"
     gene            complement(8007..8186)
                     /gene="ssrS"
                     /locus_tag="PKI64_07280"
     ncRNA           complement(8007..8186)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKI64_07280"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(8313..9263)
                     /locus_tag="PKI64_07285"
     CDS             complement(8313..9263)
                     /locus_tag="PKI64_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE00847.1"
                     /translation="MVSGDQCLVTGGAGFIGSNLVHRLVRDGARVRVLDNLSSGHREN
                     LAGVAADVEFVLGDLRDAAAVRAAARGVRHVFHIGALASVQASVDDPATTHEINVTGT
                     LNVLLAAREAGVERVVFSSSASVYGDSSEMPKRETMPPAPLTGYALSKLAGEHYGRIF
                     HGLFGLSFFALRYFNVFGPRQDPASHYAAVIPLFMCAYAAGRQPTIFGDGSQTRDFTF
                     VEDIVQANLACLTAPAAAAGRVYNVAYGERISILDLAREIAALAGQPCAPQFAPARPG
                     DIHDSQADSTLARQVLGWQPAHTFTAGLRATFDWFVQHQK"
     gene            complement(9266..>9877)
                     /gene="lptD"
                     /locus_tag="PKI64_07290"
     CDS             complement(9266..>9877)
                     /gene="lptD"
                     /locus_tag="PKI64_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOE00848.1"
                     /translation="VKPEELWQFDSIDRLDERNDLRLGLRNYLQTKREYRTHDLVFAD
                     VFTTLLIDPDEQERTLNDIGFKVETRLVSWFAWDFDGEYDTRDSSIRTFTTQAQLSQE
                     DIVTLGVDYRYKRDSRDTIAGDLVVFPEQRWSGRIYARMDLEASQVEEHSYFVIHRTR
                     CLGIGLGVRIRPEQAANGDDSYTFWLRIWPLALPQFASSLGGG"
     assembly_gap    9878..9977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9978..11200)
                     /locus_tag="PKI64_07295"
     CDS             complement(<9978..11200)
                     /locus_tag="PKI64_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOE00849.1"
                     /translation="MTGLVDVQADDLSYDGIRQLVIARGNVKVTRGADSVSADYAEVD
                     TAAQQVHARGNIFIEFQGNTWRGDEATYNFRTGQGDFGAFEVFAPPYHVTARDSRRLS
                     LNMMELEGVMLTTCDPEHPEYSVRASSASLQDNKILRAKHVRFQFGPVPFFWFPYVRA
                     NLEELAKFEFTPGWSSDNGPFLLTAYNQPINDTFKTRTHVDIRQKRGLGIGEDLLWKD
                     PRQGDYAGQLRLYYANDRRPWHDEKQQKEREELIDENRYWVQLRDRHNVTDRDYLITE
                     LNYVSDPWLLNDFFDDEYQKNVQPENRVVLSHRGDHYTAGVLLNLRLNDFYENVDRLP
                     EVFLNFNRQQILDTPLYYEGGNTLSYLSRVYPAGSPKERYDSFRFDTRHMLYWPGRYF
                     GFLSLIPRAGYRGTF"
     gene            complement(11671..>12790)
                     /locus_tag="PKI64_07300"
     CDS             complement(11671..>12790)
                     /locus_tag="PKI64_07300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE00850.1"
                     /translation="EKESQARAYDRIQGQLFLLRFGLLFAAAAAFWLSGLAEAVAAGL
                     ATRFTFPLAWPLVCAGVTALAVAGYEVLLFPLSVLADYSIERAYGRFRGEFGQWLRGY
                     LLSMLFEIGLLSGAFTGLYALHALLPNTWWLMATWLYGTLVGGLGKLGPAWLLPRVRP
                     PVPITDEDLLAELRRVGKLAGLEITGAAWWNFDYQDDLDAVQLTGWGRRRQVVFTERA
                     WRELDRRELVFLAARLMGWEQRHAALGMHLLHLTLTAAVFWGGGIIVAALAQARGYDA
                     IHPAILPFWVVVLFGLAAVAGVVVHAVGRRGELRADQFALRHVGGADTLQTCLRHEFE
                     REPFALDAAWWQVLLLRKTPTPAHRLAQAAALPRATDV"
BASE COUNT         2390 a         4098 c         4004 g         2198 t
ORIGIN      
        1 ttcggaaaaa tggcggacac gccaaattga ttgtcgagga atccgcaagc gagtcgcggg
       61 aaccgctggc gatcagctca aggccagttt ggcggtgccg ccgagaactt gcggcaaggg
      121 ttgaaaggcc ccgctgtgcc gagtggcacc cgtgcttctg gcttttttag cagcgactag
      181 aaaaagcctt tgccggatgc caccgccttc tggagttggc cgggcaatat gccgctggcg
      241 ccattcaggc tacatgaact caacacaaat ccgcctcggc gccgcgccat tcctggccca
      301 accaccagaa aaagaaaaag taaggcgctt gcccggatgg gcgtggcggc tggtggcggc
      361 ggcgggttag agcgcgggcg gctggagggc ggcatcctgc gcgcggcgga attcggcggc
      421 cttgaccatc atttccgcaa gggaatggca gccgagcttg cgcttgatgt tgccgcggtg
      481 gacttccacc gtgcggccgc tgaggccaaa cttttcggcg atatcgcgcg tagtgaggcc
      541 gctgcccatg gaggcgagga tttcgctttc acgggcggtg agcgcgccga agccatcggc
      601 cgccttgccg gcgctttccg ccgagccggc cagcagggcg gcggcagcgg ggctcaggtg
      661 cgtgccaccg gccagtactg tccgcagcgc ggccaccagc gtttcctcgg cctggtcttt
      721 catgacgtag ccttgggcgc cggcgcgcag ggctttgtcg gcgtagaggt cttcgtcgtg
      781 catggagagc accagaatct tgccggtgta acccttggcg cgcgcctcgc ggatccaatc
      841 gagcccgctt tggtcgcgca gcgaaagatc caacaccacg ccggctggtt gcagcgccag
      901 cacggcggcg aggccatcgg cataggtgcc ggcttcgccg acgatagtga aatcgctctc
      961 gcgcgtaagc agggcggcga ggccctggcg cacgatggcg tgatcatcca ccaacagcag
     1021 ggaatatggc acggcgggcg gcatgactgg tcaatcgcgc tctgcgcgcg gatcgcggct
     1081 catcagttcc accacggcct ccttgggccg cttgccgtgg tgcaagatgg catccacctg
     1141 ttccatgacg ggtaccgcca cgccaagttg gcgggcgaga gccaaggcgg tgccgctggt
     1201 ccagacgccc tcggccacct gctccatgcc gcgcaaaata tccgccagcg tttcgccgcg
     1261 gcccaagcgt tcgcccacgc cgcgattgcg gctttgcggg ctcatgcagg tgacgagcaa
     1321 atcgccgata ccgctcaagc cttggaaggt ttgcgggtgc gcgccgaggt ccacaccgaa
     1381 gcgcgtcatt tcggcgagac cgcgggtgat gagcgcggct ttggcgttat ggccgaagcc
     1441 gaggccgtcg cacacgccgg cagcaatggc gatgacgttc ttcaacgcgc cgccgacttc
     1501 cactccgcag acatcgtcgc tggtatatac gcggaaggtc aggccggtga gcaattcctg
     1561 gaagcgcgcc gccacggcgg aatccgcgca cgccacggtg accgcggtgg gcacgccgcg
     1621 cgcggtttcg ggggcgatgc tcggcccgga aagcacggcg ggctgcgcca cgccccagag
     1681 ggaagtcaac agcgcgctaa tgcgttcgcg cgtgtcttcg gcaaacccct tggtggcgct
     1741 gaccaccggc ggcgaaccag cggcgtgata agccggtgca aaacgcgtta gcgtggcgcg
     1801 cacatattgc gagggcacca ccagcatgac ggcttcggcg ccggctaggg ccgtggccgg
     1861 atcggcggtc cagctcacgc tttcgggcag aggcacgccg ggcaggaact tggggttggc
     1921 gcgggtctgg cgcacggtgt cgagatactc cgcattgggc ccccaaaccg tcggcgaatg
     1981 gccgttgcga accaaagtca gagcaagcgc cgtcccccac cccccatcgc cgaggactgc
     2041 caccctcatg gcgcgggcgg ctcctgcttg ccgaccacga tttcgttggt tgccttggcg
     2101 gggtcggcgc ccttgtcata gggcgtggtc agatacacat gcttgatcca ggaattgaaa
     2161 aactcgttgc ggttggcgcg ccagttattg atgggatttt gctcatccag attttcgagc
     2221 gggccgacat cggcgcgggc cttaaggcgg tcgcgctgtg cttcggccag cagccggctg
     2281 gaattatatt cctgatggcc gaggtgcatg atcacttggt ggtcgggggt ctcgaaaatc
     2341 gtgtagccgc ccttgtcggc gtgggccagt agccgcacct tgccggcggc ggcggcggct
     2401 tcgagcacgt catcggccac gccggaatgg cggctttgcg ggcaccagaa gacgtcatcg
     2461 agcccgccca tgatgggatg gtccggcacc agattgcgcg tgggatagac cccgaagatt
     2521 ttttgcggat acttcatttt ttcgatgccg agatatttgg ccagagccag cccgccccag
     2581 caaatgccaa gcaggcaggt gccgccgcgc atagcggcat caaaaatacc gcagagctcc
     2641 ggccaatagg tcacctgttc ccacggcagc tcttccaccg gcgcgccggt gacgatcagg
     2701 ccgtcgagcg cagccacgga ttgcgcccaa tcgaagggca ggtagttttt gtccaagtgg
     2761 tcttggtcgg agctggtata ggaatggttg cgcagccgga tccagatggg aatgatctgc
     2821 aaaatgctac ggcccatcgg ggcgagcaga ttgaactcat agtcctcggc gcgcggcatg
     2881 atgttgagga tgccgatgtg cagcgggcga atatcctgcc gctcggcggc ctcctcactg
     2941 agccaggcca cgtggtgctg ttcgaacaac ggcttgagcc gatgattggg cgacaaacga
     3001 atggtcacgg gttattcctc acaaacgagt aatcctgcca ctgggcggcg ggggctgtcc
     3061 accttagaag tacaaatcgc gctcgccggc gacggtgcgc tgatagttgg cccaaaatgc
     3121 cggccacgcg cgcggctgct gctggcgcac ctcttccaac tcgcgcgcca gcagggcttc
     3181 accaacggcc tgcgtttcgg gcgtggcgaa gtcatcgagc cattccttga aggtcaggat
     3241 ggcattgagc ttgcaaaact tgccttccac gccggtgcgc aacaggtcca tgatacactt
     3301 gccggtgcgc ccgcagcggt agcccgcggt gcagaagctg gtgatgtgcc cggcgtgggc
     3361 ccactcgcgc acgacttcat cgaggccgcg cggatcgccc aacaggaatt gctggcgctc
     3421 gcctttttgt ttttcctggc cgtcggcata ggcgcccacg ccgatgttgg aagccgcgtc
     3481 catttgggtg acccccagcg gcagcaggcg gcggcgcatg gctggtgttt cccgcgcggt
     3541 gatgatgagg ccggcgtagg gcacggccag ccgcgccagc accacgatac gctcaaaatc
     3601 gtcgtcgctc actcgccacg gcgattgctc attgaacggc gtgttgaccg ccggctccag
     3661 ccgcggaaat gaaagcgtat gcgggccaat gccgcagaac cgctccatct cgatggcgtg
     3721 cgccaactgg cccagcaact cgaagcgcca atcggtcagg ccgtaaagcg cgccgatgcc
     3781 gttgtcgtca atgcccgcct cctgggcgcg gtgcatggcg tagaggcgcc accggaaatc
     3841 gctcttgatc gtgccggcgg gatgcaggcg cgcgtaggcg cggcggtcat aagtttcttg
     3901 aaacacttgg aaggttccca gcccgcattc gtgcagcacc ttcagctcgg ccacgctcat
     3961 cggcgcagcg ttcacgttca cgcggcggat ttggcccatg ccgcggcgcg tggacacccg
     4021 caccgaatac accgtgcgaa tgctctccgc gatgtagtcc acgcccgatt ccggatgctc
     4081 accatagacc acgatcaacc gcttgtgccc gagtttgccg gccagcgctt cggtttcagc
     4141 acgtacttcc gccatgttca gcacgcggcg ttccacggcc gggttggagc ggcgcagacc
     4201 gcaatagacg cagtcattga cgcacttgct gctcatgtag agcggagcaa aagtcacgat
     4261 gcggttgtca taaaccttgc gtttgacggc cccggccgcc gtgcgcattt ccgcccacag
     4321 ctccggatcg cgcacgcgca tcagcgtggc caattcggcc tggctcagcg tttgcaccgc
     4381 caacgccttg gcgatgattt cgcgcaccgc gccggcctcc ggctcgccgg tgatgaccaa
     4441 gaggcgctca atgtcggcat cgttgatgaa atcctgccca tccgcgggca ggtatttctc
     4501 gatttccgcg gggacgatcc gttcccgtag ccactgcttt tgttcaaccg ccaacatgtg
     4561 ccaacacaat cctttccaaa tcgttcgcgc gcgcgacaac cgtgcgcccc cggcgactcc
     4621 gcggggtaaa acggttttat caaatcatca ggttaatcta cttcatcccc gtgggtttct
     4681 caactgattc gcggcgctct tttccttgtc aaagcggatg gtcgcaccta ttctctttcg
     4741 gcaataacgc acaaaaaagg aagcttcatg aacacccccg ccacccccca gagccccctg
     4801 cgcatggtca tgctcatcac gctgccgatc gttgggattt tgctcgccat catcattgcc
     4861 ttggtcatgc gcaagccaca cgacaacgcc cgcgtcaccg tcgtggtcac cgatggccag
     4921 cccggccttg acgtcaacac aggcgccatt ctcgatgcca ccgtgaccga ccagcccgtg
     4981 ctgccccgcg tgggctaccg ctataaggta tttgtggatg acgagagcga tgaccgctcc
     5041 tccgggatcg ccaaaatcgg cggccgggcc acattcattc ccggcgcccg ccgcggccaa
     5101 acggccattg tggatgtgac ccgcgtgcgc gaccgcgccg tggatgccat catggtgaag
     5161 gtgctctccg aggtggccct gccgcccaag gtgctgcgcc ccgcctttgt gccccagccc
     5221 ggcgattccg ccgcgcatgt gatgcccggc gcggagatgg atgtggtcat taccgaagcc
     5281 tccgagcgca atcctgacac cgagggcgtg gcgaaagtca gcggccttgt ggtctttgtg
     5341 cgtggcgcca cgcaaatcgg cgagcgcgtc aatgtgcgca ttaccgagcg tcgcgaaaaa
     5401 atggcgtttg ccatgctgac cggcaaaccc gccggcacgg agccgctgcc tgctgccgcg
     5461 ccggaccgcc cggcttatgt gccgccgccc ggcgaccccg ttgtggtcgg cgcggaaatg
     5521 gatgtgcaga tcaccgaggc ctccgaaaag aatcccgaca ccgaaggcgt ggcgcgaatc
     5581 ggcgggctag ttgtgtttgt gaagggcgtc accgccatcg gcgaacgcgt caatgtccgg
     5641 attgtggaac gccggcccaa gtctgcattt gcggagccca ctggcaaacc ggccggcgca
     5701 gccccgctgg cctcgcaagg caccaccggc gatatcctgc ggcgcgcatt cacccccaag
     5761 gccggcgacc ctgccggcca cgtgattgcc ggcgccgaaa tggatgtggt cattaccgaa
     5821 gcctcgcagc agcgtcccgg ccaggaaggc gtggccaaag tggccggcct cgtggttttt
     5881 gtggatggcg ccaccaccat cggcgagcgc gtcaacgtcc gcatcacagc ccggcgcgag
     5941 cgcatggcgt ttgcggaact gaccggcaag ccggcgggca ccgaacccct gcctgccgcc
     6001 gccccaagcg cgcccccccc tcccccccgc ccctacgtgc cgcccgcgga tgacgccacg
     6061 gcgttcatcg tgcccggcgc ggtgatcagc gccaccatca gcgaggcttc cgaaaagaat
     6121 cccgaaaccg aaggcgtggc gcgcgtggcc ggattagtcg tgtccgtcaa aggcgccacc
     6181 accatcggcg agacggtcaa cgtccgcatc accgagcgcc gccgcagcat ggccctcggc
     6241 gaaaagacca cggatgccgt gaccgcccac cccgccgact aacccggatt ttacgccacc
     6301 cagccccgga ggacgccatg gctcaaaccc tcaccaacga aaccaatctc cgcacccgcc
     6361 aagcggccat cgagcgcgag ctgacgtggc tcaagcgtat tcgcgtcatc gagtggggcg
     6421 gcgccatcct ttttgccttg atcggcctgg ctctgcgcca gatgggaatt gcggccgcca
     6481 actttgtgct gttcattgcc ggcgtgctcg tttttctggc cttgggccat gaagcccgcc
     6541 gtcgcgaaca aatcagcgat cttgccgaca ctgccgacct ccgcggcgtg aaggaaaagt
     6601 ttgcccaact gcttgatgaa aaactcgcca acgatcactt tgtgctcaac gacctgcacc
     6661 tgattgtcgg ccgcgaaaaa tgctggattg atcacctggt ggtcgcgccc tccgggatgt
     6721 tcgtcattga tatcctgaac tgggacggca ccatcgtggg cgacaccggc aagcaggtcc
     6781 acacatggaa aatccgcacg ccggacggca aagtgcgccc cgctcgcaat cccgtcagcc
     6841 gaacattacg cgggcgccgc atcctgtgca actggctcaa gggaaccaac ctgatttggg
     6901 aacgcgtgtt cccgctggtg gtcctggcga atcccgacgc ggatattcaa gtcaccaacg
     6961 ctgcccgccg cgtcctcacc cccgaacagg cggtgacgtt catcaacgac ttctgcttcg
     7021 agaagccggt gctttccaac gccgaagttg aaaagctcgc ccgcggcctc aaccagcata
     7081 cgaccggcgc ttgagcgccg ggcctcgccc gtggccaaga ttctccgcaa tgaaagcagc
     7141 ctgcgccgcc ggcaggacgc catccggcgc gagctggagt tccgccagat cacccgcatt
     7201 attgcctgga gcatcgtgct ggccatcgtg gtgtgggggc tgttgaccac catccgcggc
     7261 ggcagctttg cgggcgtggt agtggccggc gtagtcatca tgctcggcgc cggctatgaa
     7321 atgcgcctgc gcgaaatcga aaaagaaatc cgcaacatcg aaggcggccg gcgcggcgaa
     7381 caaaaaatgg ccgagcgcct cgccgagcag cttgccgatg atcatgtcat cctcaacgat
     7441 ctcgacttgc gcatcgccca cgagcgcggg caaattgatc acctcattat cgcgccctcc
     7501 ggcatctacg tcatggaatc caagtattgg gcgggcattt tgagcggcga ggccgatgcc
     7561 gccacctgga cccagcgccg caccaacggc ctcacgcgac gcgtcaaaag ccccgttcag
     7621 cagtgcgaac ggcagcggcg gatgttcatc accctgctgg ccaagcgtgt gccggacgac
     7681 cacgtccacg ccatggcagt cttcacccac ccctcggtgg aactgcacat tgccaacggc
     7741 gaaaaccgtg cattcctgat ccgcgacgcc atccgcttca taaacgaccg ctgttttgag
     7801 ccacccgtgc tcacccccga gcaggtgcaa gaaattgccg aatcggtcct gcgccagcag
     7861 acctgagggg agttccgcct tggaacaccc aacaatttca ctgtttttgt cgggcgggaa
     7921 tggttgaggg acgagacctt cctgtcggga gcccaaccgg aatcggtttt tgtgaccagg
     7981 tccctcggcg ggggggtgag gacaaaaaaa tccccgcctt gccggtggga cgaatctcct
     8041 gaccttatgt acataaggtg ggaacctgtc gcttgcttgc cggaaattcc ggggatcgga
     8101 cacgccgtcc acaaacgaac accagctccc gttttcaatg gtgttgggta ggagaattag
     8161 gcccctgtcg tgcaaggcag ggaaaaattc ttgctcacgt tttgcagcta ccaaagaaca
     8221 tctgctctcg acatcttgaa tgtaccaccg cgcaagattt tttgcaagcg ttccggaaca
     8281 ttttttcggc gccggtgcgc gccggaaaat cgctacttct gatgctggac aaaccagtca
     8341 aatgtggcgc gcaagccggc ggtaaacgtg tgggccggtt gccagccaag gacttggcgc
     8401 gccagcgtgg aatcagcttg cgagtcgtgg atgtcgcccg ggcgagccgg ggcgaattgc
     8461 ggggcgcagg gctgcccggc cagcgcggca atttcgcggg ccaaatccag aatggaaatc
     8521 cgttcaccgt aggcaacgtt atacacccga ccagcggcag ccgccggcgc ggtgaggcag
     8581 gccagattgg cctgcacaat gtcttcgaca aacgtgaagt cgcgggtttg ggagccatcg
     8641 ccgaagatgg tgggctggcg cccggcggca tacgcgcaca tgaagagcgg gatgaccgcc
     8701 gcgtagtgcg aggcaggatc ctgccgtggg ccgaacacgt tgaagtagcg cagggcgaaa
     8761 aaggagagcc caaagaggcc gtggaaaatg cggccataat gttcgccggc cagcttggag
     8821 agcgcgtagc ccgtgagcgg ggcgggcggc atggtttcgc gcttgggcat ttcggaagaa
     8881 tcgccataga cggaagcgga agatgaaaac accacgcgct ccacgccagc ttcccgcgcg
     8941 gccagcagca cgttcagggt accggtgacg ttgatctcgt gcgtggtggc gggatcatcc
     9001 accgatgcct gcacggaggc cagcgcgccg atgtggaaaa catggcgcac gccgcgcgcg
     9061 gccgcgcgga ccgccgcggc atcgcgcagg tcgcccagca caaactccac atctgcggcc
     9121 acgccggcca gattttcgcg gtggccggaa gacagattat ccagcacccg cacgcgcgcg
     9181 ccgtcccgca cgaggcggtg caccagattc gagccgatga aaccggcccc ccccgtcacc
     9241 agacattggt cgccgctcac catgctcacc cgccgcccag tgaactggca aactgcggca
     9301 gagccaaggg ccaaatgcgg agccagaagg tgtagctgtc gtcgccgttg gcggcctgct
     9361 cgggacgaat ccgcacgcct aggccgatgc ccaggcagcg cgtgcggtga atgacaaagt
     9421 aggagtgctc ttccacttgt gaggcttcaa ggtccatgcg cgcgtagatg cgcccggacc
     9481 accgctgctc ggggaacacc accaaatcgc cggcgatggt gtcgcgcgaa tcgcgcttgt
     9541 agcggtaatc caccccaagc gtgacgatgt cttcctgcga gagttgcgcc tgcgtggtga
     9601 aggtgcggat gctgctgtcg cgggtgtcat actcgccgtc gaagtcccac gcgaaccacg
     9661 acaccagccg ggtttccacc ttgaagccga tgtcgttgag cgtgcgttcc tgttcgtcgg
     9721 ggtcaatcaa caacgtggtg aatacatcgg caaagaccag gtcgtgcgtg cggtattcgc
     9781 gtttggtttg caaatagttg cgcaggccga ggcgcaggtc gttgcgttca tccaggcggt
     9841 caatggaatc gaactgccac aactcctccg gcttgacnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnntag aacgtgccgc gatagcctgc gcgcgggatc aggctcaaga
    10021 agccgaaata gcggccgggc caatacagca tgtggcgggt gtcgaaacga aaggaatcat
    10081 agcgctcctt gggcgagccc gcaggataca cgcggctcag gtagctcagc gtgttgccgc
    10141 cctcgtaata aagcggcgtg tcgaggattt gctgccggtt gaagttgagg aacacttccg
    10201 gcaagcggtc cacgttttcg taaaaatcgt tcagccgcag attcagcagc actccggcgg
    10261 tgtagtgatc gccgcggtgc gagagcacca cgcggttttc gggctgcacg ttcttctggt
    10321 attcgtcgtc aaagaaatcg ttgagcagcc acggatcact gacgtagttg agctcggtga
    10381 tcaggtagtc gcggtcggtg acgttgtgcc ggtcgcgcaa ctggacccag tagcggttct
    10441 cgtcaatgag ctcctcgcgt tctttctgct gtttttcgtc gtgccaaggg cggcggtcgt
    10501 ttgcgtaata caaccgcagt tggccggcat aatcgccctg acgcgggtct ttccaaagca
    10561 ggtcttcacc gatacccagc ccgcgcttct ggcgaatgtc cacgtgagtg cgggttttga
    10621 aggtatcgtt gatcggctgg ttgtaggcgg tcagcaggaa cggcccgttg tccgaactcc
    10681 agcccggggt gaactcgaat ttggccagct cctcaaggtt tgcgcgcaca tacgggaacc
    10741 agaagaaagg caccggcccg aattggaaac gcacatgttt cgcgcgcagg attttgttgt
    10801 cctgcaacga ggccgaactc gcccgcactg aatattccgg atgttccgga tcgcacgtgg
    10861 tgagcatcac gccttccagt tccatcatgt tgagcgacag tcggcgcgag tcccgggcgg
    10921 tgacgtggta cggcggcgca aacacctcga atgcgccaaa gtccccttgc cccgtgcgga
    10981 agttatacgt tgcttcatcc ccgcgccacg tgttgccctg aaactcgatg aagatgttgc
    11041 cgcgcgcgtg gacttgctgg gcggcagtgt ccacctcggc atagtcggcg gaaaccgagt
    11101 ccgcaccgcg cgtcaccttg acgttgccgc gggcgattac cagttggcgg atgccgtcat
    11161 aggaaaggtc atcggcctgc acatccacca ggccggtcaa atcagcgcct gccggcgtag
    11221 caggagtggc gataggcgca ggcggtggcg caacaggcgc aacaggcgcg accggggtcg
    11281 gacgcggtgc ggccgcgggc gcggtgggtg ccggccgcgg cgccatggcc ggggcggcag
    11341 tcggggccgg ctgcacgggc cggggcgttg ccgccggggc gggcgttgcc gggcgcggcg
    11401 caacggcagg cgccgggtgc tgcaccgggg ccggcgcgtg ccgcatgacg ggttcctcgc
    11461 tctccgggcg cgaaggcgca atgcgcggcg tctgttgggc ggcggccacc cccgccatca
    11521 gggcgaacaa caggccagcg cgtgccaagc gcccagcggt ttggacaaca aaacagggct
    11581 gaaaaggatt ttgcatgtgg cgggcaatct aacagccgcg cccaagttgc gccaagtgca
    11641 atctgaactg tccgcccgcg cgcgggcgcg ctagacatcg gtggcgcgcg gcagggcggc
    11701 ggcctgcgcc aggcggtgcg ccggcgtggg cgttttgcgc agcagcagca cctgccacca
    11761 ggcggcatcc aacgcaaacg gttcacgttc gaactcgtgc cgcaaacagg tttggagcgt
    11821 atcggccccg cccacatgac gcagcgcgaa ctgatccgcg cgcagctcgc cgcgccggcc
    11881 aacggcatgc accaccaccc ccgccaccgc ggccagaccg aacagcacca ccacccaaaa
    11941 cggcaggatg gcgggatgga tggcgtcata gccacgcgcc tgcgccaacg cagccacgat
    12001 gatccccccg ccccagaaaa cggcggcggt cagggtcaaa tgcagcaggt gcatgcccag
    12061 cgccgcatga cgctgttccc accccatgag ccgcgcggcc agaaacacca gctcgcgccg
    12121 atccaactcg cgccacgcgc gctcggtgaa caccacttgc cgccgacgcc cccacccggt
    12181 cagttgcacg gcatcgaggt cgtcttgata atcaaaattc caccacgccg cgccggtaat
    12241 ctccaagccg gcaagtttcc ccacccgccg caattcggcc agcaaatcct catcggtgat
    12301 gggtaccggg gggcgcaccc gcggcagcaa ccacgcgggc cccagtttcc ccaagccgcc
    12361 aaccagtgtt ccgtagagcc aggtggccat cagccaccac gtgttcggca gcagcgcatg
    12421 cagcgcataa agcccggtga aggcgccgct gagcaggccg atttcaaaca acatgctcag
    12481 caagtagccg cgtagccact ggccgaactc gccgcgaaac cggccatagg cgcgctcgat
    12541 ggaataatcg gccagcacgg agagcgggaa cagcagcact tcgtagcccg ccacggccag
    12601 cgccgtcacc cccgcgcaca ccaagggcca cgccaaagga aacgtgaagc gcgtggccaa
    12661 gccagccgca accgcttctg ccaagcccga tagccaaaac gccgctgccg cggcaaagag
    12721 caagccaaag cgcaacagga acagttgccc ctgaatgcgg tcataggcgc gggcttgtga
    12781 ttctttttct
//
LOCUS       DAOPNP010000069        12726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5738_length_12726_cov_9.200063, whole genome shotgun sequence.
ACCESSION   DAOPNP010000069 DAOPNP010000000
VERSION     DAOPNP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12726
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5738_length_12726_cov_9.200063"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..195
                     /locus_tag="PKI64_07305"
     tRNA            120..195
                     /locus_tag="PKI64_07305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Phe,seq:gaa)
     gene            321..1178
                     /locus_tag="PKI64_07310"
     CDS             321..1178
                     /locus_tag="PKI64_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOE00851.1"
                     /translation="MNQILFKEQLAPNVYRLRVHAPLIAEERQPGQFVILQLDTEFGE
                     RIPLTIADANLAEGSITLIFQAVGKSTKLLAQMQTGEKIANLVGPLGRPTHIAKFGTV
                     VCVGGGIGVAPLYPIAQAMRRAGNRLVVVMGARSRELVILEPEMRALTDDVVVCTDDG
                     SYGRKGLVTEPLRELCAQTPKPDLAVAIGPPLMMKFCAETTRPFGVPTVVSLNTIMID
                     GTGMCGGCRVTVGGRTKFVCVDGPEFDGHQVDFDQMIQRLGSYREQERLASAGCNLHD
                     EAVRKGLQE"
     gene            1175..2662
                     /gene="gltA"
                     /locus_tag="PKI64_07315"
     CDS             1175..2662
                     /gene="gltA"
                     /locus_tag="PKI64_07315"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOE00852.1"
                     /translation="MTFQTPASLQEAARQEWARIQTLPQPLKPRDRLAIPPQEMPAQD
                     PLARAKNMREVALGYFEEQMRVEASRCLQCKNAPCVAGCPVRIDIPRFIAALAVGDPA
                     TAIAVIKETSLLPAVCGRVCPQETQCQAPCTVGKSLKSVDKAVAIGRLERYAADWERL
                     QGQAAAPEVAPETGKKVAVVGSGPAGITVAADVRRAGHAVVLFEAFHKAGGVLVYGIP
                     EFRLPKEIVRAELDTLRRMGVELRTNFVVGRTRKVTDLIEKDGFDAVFIGSGAGLPKF
                     MNIPGENLVGVYSANEYLTRSNLMRAYDREHAVTPIHAAQRVAVLGGGNVAMDAARTA
                     LRLGASEVHLIYRRTEAEMPARVEEVAHAKEEGVQFHILQNAKRILGENGKVTGIECL
                     RYELGEPDASGRRRPVEIPGSEFQLPMDAVIVAIGNESNPLIEQTTPGLAVDKNGHIV
                     TDENGRTSLPRVFAGGDIVLGAATVILAMGEGRKAAAAINELLRA"
     gene            2771..4039
                     /locus_tag="PKI64_07320"
     CDS             2771..4039
                     /locus_tag="PKI64_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE00853.1"
                     /translation="MMQKKRSKAWLLWLLILAAVGAGGWWYMRGGNGNHQLNYKTVAL
                     ERGDIVQSVTANGALGAVQTVTVGSEVSGKIVELFADYNSVVTNGQILAKLDSSTYER
                     QLEQALAELDSARANLKLIAANFRRAQELRGLDLVSQADYDEAEATLAQAQATLKVRE
                     ANVSKVQVDLEKTTIVSPMDGVVISRAVDVGQTVAASLNAPTLFTLAQDLREMRIEAQ
                     ISEADVGGVAEGQAVTFTVDAFPELTFTGLVSQVRYEPVTNQGVVNYMAIVNVRNPDL
                     KLRPGMTANAAVITAKREQVVRLPNAALRFRPPENAVVAAGEPSADEPIAANQHGGEH
                     RAGKRPRSAARDAASTRKPIYLLAADGTLTRQMAELGISDGTWTEVIGPSPQVGDQVV
                     TGILSASDKESARGGSRSPFMPRPPGLRPH"
     gene            4044..4808
                     /locus_tag="PKI64_07325"
     CDS             4044..4808
                     /locus_tag="PKI64_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00854.1"
                     /translation="MTPADHPVIQLAGLDKVYQTGEVAVHAVRGVTLRVMPGEFVAIM
                     GASGSGKSTLMNLIGCLDRPTAGTYLLDGEDVSAHSPNNLARVRNQRLGFVFQGFNLL
                     SRTSALENVELPLLYAPHGHGRRELRRRALAALARVGLAERAAHHPSQLSGGQQQRVA
                     IARALVNEPALLLADEPTGNLDTRTSLDIMGIFQDLNDKGMTILMVTHEPDIARFCRR
                     AILMRDGRLVSDRPIEDRLNAAQELAAWQDAAEVGA"
     gene            4805..6022
                     /locus_tag="PKI64_07330"
     CDS             4805..6022
                     /locus_tag="PKI64_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE00855.1"
                     /translation="MKFSATWMIALRALRRNLMRTGLTMLGIIIGVAAVIAMVAIGTG
                     AREQVAASIAQIGQNTILVMSGATSRGGVRGGWGSAPTLTRQDYEAIREEIPGVIRAT
                     PDVRKGAQVMAGNQNVNTSVNGAGEDFVEIRAWGFAEGGNFSALDIKTKAKVAILGQT
                     VATNLFGDASPVGQVVRIQNVPYQVVGLLKPKGMSMMGSDQDDVVLVPWTSALVRLTG
                     TDSFRAITVQVESAEMVEQVMADITALLRQRHKIRDEGDDDFHVRSQQEIMEMATATA
                     KTMTILLGAIAGVSLLVGGIGIMNIMLVSVTERTREIGVRMAVGARGSDILLQFLVEA
                     VVLSVGGGLLGIGLGFGVAQLVSAELGWNTLVSPQSVALAFLFSAAIGIFFGFYPARK
                     AAGLDPIEALRYE"
     gene            6015..7535
                     /locus_tag="PKI64_07335"
     CDS             6015..7535
                     /locus_tag="PKI64_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE00856.1"
                     /translation="MNKFLKQSGVILGLLLIMAGAAPAQTNATQSLTLAECVAIALEH
                     NLALQIEKLSSRSAQEDVRAALGGYDPQLTLSAQRAHTEIEGKPAEDDELLVGSGSDT
                     DRDTYEAAIRGATALGGLSYEVGTKLGRNEGTRSGNPFDTSTGSAGVTLTQPLLKGFT
                     TDATRYQVTLARKLSTEALVQLETAMANTIHDVEVAWFGLVQALESIAVQEEALRLAT
                     QLYEDNRNKVQIGAMSILDEKQAESQAAAARADLSAAKQAYAEAQNRLKLLVFADHRQ
                     TATTTIAPVGELVSEPVMTDVAASSAQALEQRPELRQARLALARQDVVVAYQRDQTRP
                     ALNLVGNAGVAANDERDSSGVFRQLESANEPYWSAGITLAIPLGNRAAQARHEQSRLT
                     ADKMKLQLRQLEEQVLVEVDNAANAVAAGYERLQATRDARDYARQALLAEQRKLERGK
                     STSFVVLQLQRNLTAARNAAVQALMDYNKQCANLALAEGATLARYHITFAPPPPEK"
     gene            7611..9356
                     /locus_tag="PKI64_07340"
     CDS             7611..9356
                     /locus_tag="PKI64_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE00857.1"
                     /translation="MGRGDIKDNMLSAASGTVAAARDFDQFRVSVPCQFACPAHTDIP
                     GYLAAIAKGDYDTAYRINLRDNVFPGVLGRVCTQPCETVCRHGREGLGESVAIRVAKR
                     AAADFRRHTDPIILEPIFSASGKRVAVVGAGPAGLTTARELCRLGHAVDLYEKDDQPG
                     GLMVQGIPLFRLPREIIAHEVAQALAVGIKLHLGVSADLAELRQTHDAVVWATGAQDP
                     VLPDLPGADLPGVMHGLPFLRRVNRGEKPAIGRRVVVIGGGFTAVDCARIARRLGAEH
                     VRIFYRRSEVEMYISTDEMDALAAEGITLETRVMPLACLGDSHVKEIRLARTVPGELD
                     AHGRRQYDLVPGTEFTVAADTVLFGTGQRPIRPASTQPPVFLAGDVATGSTSLIDTIG
                     HAKRVVRAVDQFLMGARRIGDDIGIGPVVPSTGRTREMDAIPRQEMALVALTPDQPDA
                     EVEQGFTREQAMTEAIRCYQCQYLFEIDNRRCIYCDMCITACPVPECIVKVSAVHRDA
                     EGRWQGFQRATGPQDYNLLCIDPDLCIRCDLCVKACPVHCIPAQEMAFHQAAPGAAGQ
                     FEQAVANTAKESPHG"
     gene            9349..10827
                     /locus_tag="PKI64_07345"
     CDS             9349..10827
                     /locus_tag="PKI64_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE00858.1"
                     /translation="MGDDHLDLFLIFPNNRQHAYGDLGRDIAGVTPPVQLGLLAAYAR
                     RHGLTVDLLDADAAGLLPEQVAARVKAAAPALVVVGTDQVNSGDVTKMQAAGETVRAI
                     HALATDIPVLLDGVVPSAYPERILRSEEADFVCCGESYEPATKLAAWLKAHGRNRRPA
                     AGEIPGIWSRQGDEVTQGGRAPMFADVEQLPDAAWDLMPPARYRAHHWHCFDRLNARS
                     PYAAIYTNHGCPYNCSYCSVNVVAGRPNLRLRTADRVLGELETLVKLGVRNVRLLDNV
                     FTINAERVEEICDRISAKGWDLNFWAYAHVSSIRGPDLLKKLKKAGVNWLAYGFESAN
                     ARVRGSVNKMTTEDATDQVIGWTRDADISIVGNFIFGLPEDDLASMQESFDMAKKFQF
                     EWANFYCAMAYPGTRLYDELVAKGVSMPTKWSAYGHYSRDSRPLSTKYVDWKEVVRFR
                     DAAFKEYYESPAYQAMITKRFGPESAAYVRRILSQPLVRDFT"
     gene            10824..11651
                     /locus_tag="PKI64_07350"
     CDS             10824..11651
                     /locus_tag="PKI64_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOE00859.1"
                     /translation="MTSAHQHSGSLRELAARFRADTLRIHAQAPETRVASSLSAVEIY
                     TCLFYGGLLRHNPANPRDPARDRLIVSKGHGSISLYPILADRGFFSPEELARVCRPGT
                     FLGGIPDPIIPGYETVNGSLGHGPGVGTGMAIALQRQGSDRRVVVVVGDGELHEGAVW
                     EALLFAAQQKLNNLILIVDDNGRCMMDYTSHVVDLAPLPDKLRAFGWSVEEVNGHDVD
                     ALCATLRRSMESSFPGPRAVITHTIKGKGAPALEDDPLCHVRTLTPGEVEELTGGAP"
     gene            11648..12610
                     /locus_tag="PKI64_07355"
     CDS             11648..12610
                     /locus_tag="PKI64_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE00860.1"
                     /translation="MSTPKRRMRDAFLQVIKDRMGAESDIFFLTGDFGSPVLDGVRRD
                     FADRFLNVGIAEQNLVNVATGLALEGYRVIAYAIAPFITMRCLEQIRINLAILSQVRP
                     MNVTLVGVGAGYSYEVSGPTHQALEDMALMRALPNIGVWSPADAATAAAMANRALDTP
                     GVRYVRLDSHPLPDLGPAPTPQELQCGFRLSGTTGAAAALVSTGYLSHLAAGVRDELE
                     AGGLPVLTVDMMDLAHADMTELTTVLAPCRNILTMEEGFIGCGGMDALLMLHATPRLP
                     KAKWAHAGLQPHYHFEIGPRDALLAAQGISRSALAARMIELINR"
BASE COUNT         2375 a         3762 c         4234 g         2355 t
ORIGIN      
        1 tttcccccaa aaaaaagaga gattttcact taaccaactt ttatttcgtg caccctgggc
       61 ggtgtgctgt attgacagcg ggggggcctt gccgttatac acagcggaac agttttggtg
      121 gcgagatagc tcagctggta gagcaacgga ctgaaaatcc gtgtgtcacc ggttcgatcc
      181 cggttctcgc caccattttt tttgtgcccg tgcggggcgg gatgggcaaa gcgttttcct
      241 gcgccgggcg gaaaattctt tgcgggtggc agggggatgg gtaggatggg cgggttgttt
      301 gtcggcagga ggctagacgc atgaaccaga ttcttttcaa ggagcagttg gcgccgaatg
      361 tgtaccgcct gcgggtgcat gcgccgctga ttgccgagga gcgccagccg gggcagttcg
      421 tgattctgca actggatacg gagtttgggg agcgtatccc gctgacgatt gccgatgcca
      481 atttggcgga aggcagcatt acgctgattt ttcaggcggt gggcaagagc accaagttgc
      541 tggcgcagat gcaaacgggg gagaaaatcg cgaatctggt ggggccgctg ggccggccaa
      601 cccatattgc caagttcggc acggtggtgt gcgtgggtgg cggcatcggc gtggcgccgc
      661 tctacccgat tgcgcaggcc atgcggaggg cgggcaaccg gctggtggtg gtgatgggcg
      721 cgcggtcgcg cgagctggtg attctggaac ccgaaatgcg ggcgttgacc gatgacgtgg
      781 tggtgtgcac ggatgacggc agctatggcc gcaagggcct cgtgaccgaa ccgctgcgag
      841 aactgtgcgc tcaaacgccc aaaccggacc tggccgtggc gattggcccg ccgctgatga
      901 tgaaattctg cgcggaaacc acgcggccct tcggcgtgcc cacggtggtg agtttgaata
      961 ccatcatgat tgatggcacg gggatgtgcg ggggctgccg ggtgacggtg ggcgggcgca
     1021 ccaagtttgt atgcgtggat ggcccggagt ttgacggcca ccaagtggat tttgaccaga
     1081 tgattcagcg gctgggttcc taccgcgagc aggagcgctt ggcctcggcc ggctgcaacc
     1141 tgcacgacga agccgtgcgg aagggtttgc aagaatgact ttccaaacgc ccgcctcctt
     1201 gcaggaggcc gcgcgccagg agtgggcgcg catccaaact ctgccgcaac cgttgaagcc
     1261 acgtgaccgc ttggccattc cgccgcagga gatgccggcg caagacccgc tggcgcgggc
     1321 caaaaacatg cgcgaggtgg cgctgggtta ttttgaagag caaatgcgcg tggaggcatc
     1381 gcgctgcttg cagtgcaaaa acgcgccgtg cgtggccggc tgtccggtgc ggattgatat
     1441 tccccgtttt atcgcggcgc tggcggtggg cgatccggcc acggccattg cggtcatcaa
     1501 ggaaacaagc ctgttgccgg cggtgtgcgg tcgcgtatgc ccgcaggaaa cgcaatgtca
     1561 ggccccgtgc accgtgggca agagcttgaa atccgtggat aaagccgtgg ccatcggccg
     1621 tctggaacgc tacgccgcgg actgggaacg cttgcagggg caggccgcgg cgccggaggt
     1681 ggcgccggaa accggcaaaa aagtggcggt ggtcggcagc ggcccggcag gaattaccgt
     1741 ggctgcggat gtgcgccgcg cggggcacgc cgtggtgctg ttcgaggcct tccacaaggc
     1801 cggcggcgtg ctggtgtatg gcattccgga gttccgcctg cccaaggaaa tcgtgcgcgc
     1861 cgagctggat actctgcggc gaatgggcgt ggaactgcgt accaactttg tggtagggcg
     1921 cacccgcaag gtcacggacc tcatcgaaaa agacggcttc gatgcggtat tcatcggctc
     1981 gggcgcgggg ctgcccaagt tcatgaacat ccccggcgag aatctggtgg gggtgtattc
     2041 cgccaacgaa tatctgacgc gctccaacct gatgcgagcc tatgaccgcg agcacgccgt
     2101 cacgccgatc catgccgcgc agcgcgtggc cgtgctgggt ggcggcaacg tggcgatgga
     2161 tgccgcgcgc accgcgttgc gcctcggcgc gagcgaagta cacctgattt accggcgcac
     2221 cgaagcggag atgccggcgc gcgtcgagga agtggcccac gccaaggaag aaggtgtgca
     2281 attccacatt ctgcaaaatg ccaaacgcat cctcggcgag aacggcaagg tgacgggcat
     2341 cgaatgcctg cgctatgaac tgggcgagcc ggatgccagc gggcggcggc gccccgtgga
     2401 aatccccggc agtgaattcc agctccccat ggatgccgtg atcgtcgcca ttggcaacga
     2461 atccaatccc ttgatcgagc aaaccacacc cgggttggcg gtggataaaa acggtcatat
     2521 cgtgacggat gaaaacgggc gcaccagcct gccgcgcgtt tttgccggcg gggacatcgt
     2581 gctgggcgca gccaccgtca ttctggcgat gggcgaaggc cgcaaggccg ccgccgccat
     2641 caatgagctg ctgcgggcct gacccctgcc cccccccctg cggggcaaac gggattggag
     2701 aaaggcctgc gtcgaatgta accttggcta ctggacgggc gactgatcta ctaatacccc
     2761 tgtggagaac atgatgcaga aaaagcgaag caaggcgtgg ctgctctggc tgctgatatt
     2821 ggcggccgtg ggcgccgggg gttggtggta tatgcgcggg ggaaatggaa accaccagct
     2881 caattataaa acagtcgcgt tggagcgcgg cgatatcgtg cagtccgtga cggccaacgg
     2941 cgcgctgggc gcggtgcaaa cggtcacggt gggcagcgag gtttccggca aaattgtgga
     3001 gctgttcgcg gattataatt cggtggtcac caacggccag attctcgcca agctggattc
     3061 ctccacctac gagcggcaac tcgagcaggc gctggcggag ttggacagcg cgcgggccaa
     3121 tctgaaactg attgccgcca atttccggcg cgcgcaggag ctgcgggggt tggatttggt
     3181 gagccaagcg gattacgatg aagccgaggc caccctcgcg caggcccaag ccacgctgaa
     3241 agttcgcgag gccaacgtca gcaaagtgca ggtggatttg gaaaaaacca ccatcgtttc
     3301 accgatggat ggcgtggtga tttcgcgcgc ggtggatgtg ggccaaaccg tggccgccag
     3361 cctgaacgcc cccaccctgt tcacgttggc gcaggacttg cgcgaaatgc gcattgaagc
     3421 gcagatttcc gaggccgatg tgggcggagt ggcggaaggg caggccgtga cgttcaccgt
     3481 ggatgccttc cccgagttga cgttcaccgg gctggtgagt caggtgcgct atgaaccagt
     3541 gaccaaccaa ggcgtggtga actacatggc cattgtcaac gtccgcaacc ccgacttgaa
     3601 actgcgaccg ggcatgaccg ccaatgcggc ggtgattacc gccaagcgcg agcaagtggt
     3661 gcggctgccg aatgccgcgc tgcgcttccg cccgcccgag aatgcagtgg tggcagcggg
     3721 tgaaccatcg gcagatgaac ccatcgctgc caaccagcac ggcggtgaac accgcgcggg
     3781 gaaacgcccg cgatccgcgg cgcgagatgc ggcttcgact cgcaagccaa tttatctgtt
     3841 ggcggcagat ggcacgctga cccggcaaat ggccgaactc ggcatcagcg atggcacctg
     3901 gacggaagtg atcggcccca gcccccaagt tggcgatcag gtggtaactg gcattttgtc
     3961 cgcttctgac aaagaatccg cgcgcggggg ctcccgctcg ccttttatgc cgcgcccgcc
     4021 cggcctccgc ccgcactaag ccgatgacgc ccgccgatca tcccgtcatt caactggccg
     4081 gcctggataa ggtgtaccag accggcgagg tggcggtgca tgccgtgcgc ggtgtgacgc
     4141 tgcgcgtgat gcccggcgaa tttgtcgcca tcatgggcgc cagcggttca ggcaagtcca
     4201 ccttgatgaa tctgatcggc tgcctcgacc ggccgaccgc cggcacctat ttgctggatg
     4261 gcgaagatgt cagcgcgcac tcgccgaaca atttggcgcg ggtgcgcaac cagcggttgg
     4321 gctttgtatt tcagggcttc aatctgctct cgcgcacctc ggcgctggaa aatgtggagc
     4381 tgccgctgct ctacgccccg catggtcacg gccgccgtga gttgcgtcgc cgcgccctgg
     4441 cggcgctggc gcgggtgggc ttggccgagc gcgccgcgca ccacccgagc caattgtcgg
     4501 gcggccaaca acagcgggtg gccatcgccc gtgccctggt gaacgaaccc gcgctgctgc
     4561 tggcggatga gcccaccggc aatctcgata cgcgcaccag cctcgacatc atgggtattt
     4621 tccaagactt gaatgacaag ggcatgacca tcctcatggt gacccatgaa ccggatatcg
     4681 cgcggttttg tcggcgcgcc attctgatgc gcgatggccg cctcgtcagt gaccgcccca
     4741 tcgaggaccg gttgaatgcg gcacaagaat tggccgcgtg gcaggatgcc gcggaggtgg
     4801 gcgcatgaag ttttccgcca cgtggatgat tgcgctgcgc gcgctgcgcc ggaatctgat
     4861 gcgcaccggg ctgaccatgc tgggcattat tattggcgtg gcggcggtga tcgccatggt
     4921 ggccatcggc accggcgcgc gggagcaggt ggcggccagc atcgcgcaga ttgggcaaaa
     4981 caccattttg gtgatgtcgg gtgcgacgtc acgcggtggc gtgcgcggcg gttggggcag
     5041 cgcccccacc ctcacccggc aggactatga ggccatccgc gaggagattc ccggcgtaat
     5101 tcgcgccact cccgatgtgc gcaagggcgc ccaagtcatg gccggcaacc agaacgtcaa
     5161 tacctccgtc aatggcgccg gcgaagattt cgttgagatt cgtgcctggg gctttgccga
     5221 aggcggcaac ttttcggcgt tggacatcaa gaccaaggcc aaggtggcca ttttggggca
     5281 aaccgtggcc accaatctgt ttggtgacgc cagcccggtg gggcaagtgg tgcgcattca
     5341 gaatgtgccg tatcaggtgg tgggcctgct caagcccaaa ggcatgagca tgatgggctc
     5401 ggaccaggat gacgtggtgc tggtgccgtg gaccagcgcg ttggtgcggc tgaccggcac
     5461 ggacagtttc cgggccatca ccgtgcaggt ggaaagcgcc gaaatggtgg agcaagtcat
     5521 ggcggacatc accgcgctgc tgcgccagcg gcataaaatc cgcgacgaag gcgacgacga
     5581 ttttcatgtg cgtagccagc aggaaatcat ggaaatggcc accgccactg ccaaaaccat
     5641 gaccattctg ctcggagcca ttgccggagt gtcgttgctg gtgggcggga tcggcatcat
     5701 gaacatcatg ctggtgagcg tgacggagcg cacgcgcgag attggcgtgc gcatggcggt
     5761 cggtgcgcgc ggcagcgaca tcctgctgca atttctggtc gaggcggtcg tgctgagtgt
     5821 gggcggcggg ttgttgggca tcgggctggg tttcggcgtg gcgcagttgg tgtccgccga
     5881 actgggctgg aacacgctcg tctcgccgca gtcggtggcg ctggcgtttt tgttcagcgc
     5941 ggccatcggc atctttttcg gtttttatcc cgcgcgcaag gccgccgggt tggaccccat
     6001 cgaggcgttg cgctatgaat aaatttttaa aacaatcggg cgtgatcctc gggctgttgc
     6061 tgatcatggc gggcgcggcg cccgcgcaaa ccaacgcgac gcaaagcctg acgctggccg
     6121 agtgtgtggc catagcgctg gagcataatc tggcgctgca aattgaaaaa ttatcgagcc
     6181 gctcggctca agaagatgtc cgcgccgcgt tggggggcta tgatccacaa ctcaccctgt
     6241 ccgcccagcg ggcgcacacg gagatcgaag gcaagccggc ggaagacgat gagctgttgg
     6301 tgggaagtgg ctccgatacc gaccgcgata cttatgaagc cgccattcgc ggggcaaccg
     6361 cgctaggcgg attgagctat gaagttggca ccaaactggg gcgcaacgaa ggcacgcgca
     6421 gcggcaaccc atttgatacc agcactggca gcgcgggcgt gaccttgacg cagccgctgc
     6481 tcaaggggtt caccaccgat gccacgcgct atcaggttac tctcgcgcgc aaactctcca
     6541 ccgaggctct cgtgcaattg gagacggcca tggccaatac cattcacgat gtggaggtgg
     6601 cctggttcgg tttggtgcag gcgctggaga gcatcgccgt gcaggaagag gcgctgcggt
     6661 tggccaccca gctttacgag gacaaccgca acaaagtgca gatcggcgcc atgtccattc
     6721 tggatgaaaa acaggccgag agccaagcgg cggcggcgcg ggcagacttg agcgcggcca
     6781 agcaggctta tgccgaagca caaaatcgcc tcaagctgct ggtctttgcc gaccaccggc
     6841 aaacggcgac gacgaccatt gctccggtgg gcgaactggt gtcggagcca gtcatgaccg
     6901 atgtggccgc cagtagcgcg caggcgctgg agcagcggcc ggaactccgg caggcacggc
     6961 tcgctttggc gcggcaagat gtggtggtgg cctaccagcg cgaccaaacc cgtcccgcgc
     7021 tcaacttggt gggcaacgcg ggcgtggctg ccaatgatga gcgcgattca agcggcgtgt
     7081 tccgccagtt ggaatccgcc aatgagccgt actggtccgc cggcatcacg ctggccatcc
     7141 cgctgggcaa ccgcgccgca caagcccggc atgagcaaag ccggctgacc gccgacaaaa
     7201 tgaaactgca attgcgccaa ctggaagagc aggttttggt ggaggtggat aacgcggcca
     7261 acgcggttgc tgccggctat gaacggctgc aagccacccg ggatgcccgc gactatgccc
     7321 ggcaggcgct gctggccgaa cagcgcaagc tggaacgcgg caaaagcacc agctttgtcg
     7381 tgctgcaact gcaacgcaac ctgaccgccg cccgcaatgc cgcggtgcag gcgctcatgg
     7441 actacaacaa acagtgcgcc aaccttgcct tggccgaagg cgccaccctc gcccgctacc
     7501 acatcacctt cgcccccccg cccccggaaa agtaatcatt ttccgcggtg ttttcgttga
     7561 agaccggcag ggcgcgtgat aggttgccgg ttcagacaac gagcgcaggc atgggtagag
     7621 gcgacatcaa agacaatatg ctttcggccg caagcggcac cgtggctgcc gcgcgcgatt
     7681 ttgatcagtt ccgggtgagt gtgccatgcc agttcgcttg tccggcccac accgatatcc
     7741 ccggctactt ggcggccatc gcgaagggcg actatgacac cgcctatcgc atcaacctgc
     7801 gggacaatgt atttccgggc gtgttgggcc gggtctgcac ccagccatgc gaaaccgttt
     7861 gtcggcacgg ccgcgaagga cttggcgaat cggtggccat ccgtgtggcc aagcgtgcgg
     7921 ccgccgattt ccgccggcat accgatccga ttattttgga acccattttt tcggccagcg
     7981 gcaagcgggt ggccgtggta ggcgcaggcc ctgccggatt gaccaccgcg cgcgaattat
     8041 gccgccttgg ccatgcggtg gacctctatg aaaaagatga ccagcccggg gggctaatgg
     8101 tgcagggtat tccgctgttt cgcttgccgc gggagatcat tgcccatgaa gtcgcccagg
     8161 cgctggccgt gggaatcaag cttcacctcg gcgtgagcgc cgatttggcg gaattgcgcc
     8221 agacccacga tgccgtggtg tgggccacgg gcgcccagga cccggtgctg ccggatttgc
     8281 cgggcgccga tttgccgggc gtcatgcacg ggctgccctt tttgcgacgg gtcaaccgtg
     8341 gagaaaaacc ggcgatcggg cggcgggtgg tagtgatcgg cggtgggttc acggcggtgg
     8401 attgcgcgcg gattgcgcgg cggctgggtg cggaacatgt gcggattttc tatcggcggt
     8461 ccgaagttga gatgtacatc agcaccgatg aaatggacgc cttggctgcg gaaggtatta
     8521 cgctggaaac ccgcgtgatg ccgttggcct gcctgggcga ttcgcacgta aaagaaatcc
     8581 gtctggcccg caccgtcccc ggtgagttgg atgcccacgg acgcaggcag tacgacctgg
     8641 ttcccggcac ggaatttacc gttgcggccg atacggtgct gtttggcacc gggcagcgtc
     8701 ccatccggcc cgcgtcaacg cagccgccgg tttttctggc cggcgatgtg gccacgggat
     8761 ccacttcgct gattgatacc atcggccacg ccaagcgcgt cgtccgcgcg gtggaccaat
     8821 tcctcatggg cgcgcggcgc atcggcgatg atatcgggat cggcccggtt gtgccttcga
     8881 ccggccgcac aagggaaatg gatgcgatcc cccggcagga gatggcgttg gtggcgctta
     8941 cgccggacca gccggatgcc gaagtggagc aaggattcac gcgggagcag gccatgacgg
     9001 aggccatccg ctgctatcag tgccaatatc tttttgaaat tgataaccgg cgttgcatct
     9061 attgcgacat gtgcatcacc gcctgcccgg tgccggagtg cattgtcaaa gtttcggcgg
     9121 tgcaccgcga tgccgaaggg cgctggcagg gctttcaacg ggccaccggt ccgcaagact
     9181 acaatttgtt gtgcattgat ccggatttgt gcatccgctg cgatttgtgc gtcaaagcct
     9241 gcccggtgca ctgcatcccc gcgcaagaga tggccttcca tcaggccgcc ccgggcgcag
     9301 ccggccaatt tgaacaagcc gtggccaaca cggcaaagga gagtccccat gggtgatgac
     9361 cacttggacc tgtttttgat tttccccaac aaccggcaac atgcctacgg tgacttgggg
     9421 cgggatatcg ccggggtcac gccgccagtc caattgggac tgctggccgc ctatgcgcgc
     9481 cggcacggcc tgacggttga tttgctggat gccgacgcgg cggggctgct gcccgaacag
     9541 gtggcggcac gggtcaaggc ggccgcgccg gcactggttg tggtcggcac cgaccaagtc
     9601 aactcgggcg atgtgaccaa aatgcaggca gcgggcgaaa cagtccgcgc gatccatgcg
     9661 ctggccacgg acattcccgt gctgctggat ggcgttgtgc catccgccta cccagagcgg
     9721 attttgcgca gcgaagaagc ggactttgtc tgctgcggcg agtcttacga gccggccacg
     9781 aagttggcgg cctggctaaa ggcccatggc cggaaccgcc ggccggccgc gggagaaatt
     9841 cccggcattt ggtcgcggca gggcgatgaa gtgacccaag gggggcgcgc accgatgttt
     9901 gcggatgtgg agcaattgcc ggacgcggcc tgggacctga tgccgcccgc ccgttaccgg
     9961 gcccaccatt ggcattgctt tgaccggctg aacgcgcgca gtccttacgc cgcgatttat
    10021 acgaaccacg gctgccccta caactgctcc tattgcagcg tgaacgtcgt tgccggccgc
    10081 ccgaacctgc gcttgcgcac cgccgaccgg gtgctgggcg agttggaaac gctggtgaag
    10141 ctgggggtgc gcaacgtgcg cctgctggac aatgtgttca ccatcaatgc tgaacgcgtc
    10201 gaagaaattt gcgaccggat tagcgccaag ggctgggacc tgaatttctg ggcgtatgcc
    10261 cacgtgtcat ccatccgcgg gcccgacctc ttgaagaagc tgaagaaagc cggcgtgaat
    10321 tggctggcct acggctttga atccgccaac gcacgggtgc ggggctcggt caacaaaatg
    10381 accactgaag atgccacgga ccaagtgatc ggctggaccc gcgacgccga tatttccatc
    10441 gtcggcaatt tcatcttcgg gctgccggag gatgatttgg cgagcatgca ggaatcattc
    10501 gacatggcca aaaagttcca gtttgaatgg gccaacttct attgcgcgat ggcctatccc
    10561 ggcacacggt tatatgacga attggtggcc aaaggcgtgt ccatgcccac gaagtggtcg
    10621 gcctacgggc actacagccg ggactcacgc ccgctctcca ccaagtatgt tgactggaag
    10681 gaagtcgtgc gtttccgcga tgcagcgttc aaggaatact acgaatcacc ggcctatcag
    10741 gcgatgatca ccaaacgctt tggccctgag tcggcggcct atgtgcggcg gattctctcc
    10801 caaccgctgg tcagggattt tacatgacca gcgcccacca gcattccggt tccttgcgtg
    10861 agctggcggc acggttccgc gcggacacct tgcggattca cgcgcaagcg ccggaaaccc
    10921 gcgtggcgtc ttccctgtcg gccgtggaaa tctatacctg cctgttctat ggcgggctgt
    10981 tgcggcacaa cccggcgaac ccgcgggacc ccgcgcggga ccggctgatc gtcagcaagg
    11041 ggcacggctc catcagcttg taccccatct tggccgaccg gggattcttt tcgccggagg
    11101 agctggcgcg cgtctgccgg ccgggcacat tcctcggcgg cattcccgac cccatcatcc
    11161 ccggttacga aaccgtcaat ggctcattgg gccatggccc cggcgttggc acgggcatgg
    11221 ccattgcgtt gcaacggcaa ggttcagacc gccgcgtggt ggtggtggtc ggcgatggcg
    11281 aactgcacga gggcgccgtg tgggaagccc tgttgtttgc ggcccagcag aagttgaaca
    11341 acctgattct gattgtggat gacaacggcc ggtgcatgat ggattacacc tcccacgtgg
    11401 tcgatcttgc gccgctgccg gataaattac gcgcgttcgg ctggtcggtt gaggaggtca
    11461 acggacacga tgtggatgct ttgtgcgcca ccctgcgccg cagcatggaa agcagcttcc
    11521 ccggaccgcg cgcggtcatc acgcacacga tcaaaggcaa aggggccccg gccttggaag
    11581 acgatccgct gtgccacgtc cgcaccctga ctccgggcga agtggaagaa ttgacgggag
    11641 gcgcgccgtg agcactccca aacgccgcat gcgtgacgcg tttcttcagg tcatcaagga
    11701 ccggatgggc gcggaatccg atattttctt tttaaccggt gattttggtt cgccggttct
    11761 cgatggtgtg cgccgggatt ttgcggaccg ttttctcaac gtgggcatcg ccgagcagaa
    11821 tctggtgaat gtggcgaccg gcctggccct cgaaggctat cgcgtcattg cctatgccat
    11881 tgcgccgttt atcaccatgc ggtgcttgga gcagattcgc atcaatctgg ccatcctctc
    11941 ccaagtccgg ccgatgaatg tgacgctggt gggcgtgggc gccggctaca gttatgaagt
    12001 gtccggcccg actcatcagg ccttggaaga catggccctg atgcgggccc tgccgaatat
    12061 cggggtctgg tcccccgcgg atgcggccac tgctgccgcc atggcgaacc gggcgctgga
    12121 caccccgggc gtgcgctatg tgcgcctcga cagccatccg ctgccggatc tcggccccgc
    12181 accgacaccg caagaacttc aatgcggctt ccgcctcagc ggcactacgg gtgcggccgc
    12241 agcgcttgtt tccaccggct acctgagcca tctggccgcc ggggttcgcg atgaactgga
    12301 agccggcggg ctgccggtct tgactgtgga tatgatggac ttggcgcacg ctgacatgac
    12361 tgaactaacg accgtgctcg cgccctgccg caatatactg accatggaag aaggatttat
    12421 cggctgcggc ggcatggatg ctctgctgat gttgcacgcg accccacgcc tacccaaagc
    12481 caaatgggcg cacgcgggac ttcagccgca ttatcacttt gaaatcggcc cgcgcgatgc
    12541 tttgctcgcg gcacaaggga tttcacgttc cgcgctggcg gcgcgcatga tcgagctgat
    12601 caatcgttga tcctaccatt ccagtcctgc aatccaatcg gctgtttttg tagggcggga
    12661 atggttgagg gacgaaacct tcccgccggg aaaggctgga aggtctgcga ccgttccagc
    12721 cctaca
//
LOCUS       DAOPNP010000070        12503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5866_length_12503_cov_9.610861, whole genome shotgun sequence.
ACCESSION   DAOPNP010000070 DAOPNP010000000
VERSION     DAOPNP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12503
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_12503_cov_9.610861"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..874)
                     /locus_tag="PKI64_07360"
     CDS             complement(176..874)
                     /locus_tag="PKI64_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOE00861.1"
                     /translation="MRLSLTLHSVVLAGILLWPLIFRGCHRPKKPEQVMMVEFTVSIP
                     PPPEPETPPTPEPPAPEPPKPADDIKLPDPEKPKPPAPPKPPKPPEPPKDIRQTNRVV
                     RNAPPPKDKPLSPEEIARLLKQGAVVGTETKIPADDQIAFAAYLNRVRELLYAVWQQP
                     AQLASLGSLSTDVNITVAPDGTITGHSKTRGSGNALMDDSVIRAITSVKKLPALPVGY
                     RTSKTISITFQLDN"
     gene            complement(901..1308)
                     /locus_tag="PKI64_07365"
     CDS             complement(901..1308)
                     /locus_tag="PKI64_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE00862.1"
                     /translation="MARRTSLTNTRFISELNMTSIMDLTFLLLLTFMITFPNIEQGIA
                     VRLPKGKTTTLEHQDSLAITLNANAELFLDNAAITEEALANALKTKVADNPEITVLVR
                     ADETLAYGKVVNVLRLMHEAQITRMAIVTDPGK"
     gene            complement(1318..2058)
                     /locus_tag="PKI64_07370"
     CDS             complement(1318..2058)
                     /locus_tag="PKI64_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE00863.1"
                     /translation="MNVSFLTGALPLAGVLWAFNISDPVGKIIVVVLIFASVFAWSIM
                     AAKYLELRKVGRADANFMRDFDQQHNPLEMFVRGPVHHDSPMAKVYAAACLAVKREFE
                     TRAQKQQRPLSQIDLSQERLTALQIDAIRKVAECAVADQILLLETQMSMLGSTYTIAP
                     MLGLFGTVWGISVSFEQMAKQGSANLSEAAPGISSALLTTIVGLLVAIPSAFGCNKLN
                     DQIRFLSIQLENFTEKFATRLQQSFVYE"
     gene            2201..3868
                     /locus_tag="PKI64_07375"
     CDS             2201..3868
                     /locus_tag="PKI64_07375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOE00864.1"
                     /translation="MAEKKRTAETAESMAKRQREISVSEFFLKNRHLLGFDSPRKAIM
                     TAVKEAVDNSLDACEEAGILPEISVELQQQAENRFVVIITDNGPGIVRAQIPKVFAKL
                     LYGSKFHRLRMSRGQQGIGISAAGMYGQLTTGTATIILSRTRSAGNAHRLSVQIDTRA
                     NKPAVLGDEEVDWRPTFPPEAPGQEPVTHAHGTQVTIEMTAQYVRGRLSVDEYLKQCA
                     VANPHLRLHFRVTTLKKDKSGALAPETGDWQTYLRAVRTLPPVTKAIQPHPHGVELGV
                     LLQMLKDTAARTLKGALEQEFSRVSARAAQEICERAGLNPKANPTRVAHHEIEALFKA
                     IQETKLMRPPTDCLAPIGEEQILAGLKKEFPADFYAAVTRAPEVYRGNPFQVEVGVAY
                     AKPGTNAELGAEEPVRVMRFANRAPLLYMGGACAMTEALENVNWKAYGLLQPKGSLPL
                     GPVVFFLHIASVWVPFTSESKEAVAHYPEILREMTFALQEAGRQLAVYLSRRRRQAEA
                     DRKRGYIEKYIPHLALGLQEILTLSDKQKTKVEDDLTRMLERTHLET"
     gene            3947..4294
                     /gene="mscL"
                     /locus_tag="PKI64_07380"
     CDS             3947..4294
                     /gene="mscL"
                     /locus_tag="PKI64_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOE00865.1"
                     /translation="MSIIKEFQEFAVKGNMVDMAVGIIIGGAFGKIVTSLVNDVIMPP
                     LGMLLGKVDFKDLAITLKEAGVDAAGAPVEAVKLSYGLFIQNTVDFLIVAIAVFMMVK
                     AINTLRRKQEAAS"
     gene            4320..5081
                     /locus_tag="PKI64_07385"
     CDS             4320..5081
                     /locus_tag="PKI64_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOE00866.1"
                     /translation="MKKFWILLIAASLATAASAAEKKAAKAAEKPTAKPAFATTLNAG
                     LTMTDGNSETLAANASLVTEGEKEGLGSVRAGVEGNYGESTVNDEKETTVENAKVFAN
                     VKKTLTPRTFAVLDGSVLYDDVALVDYRVTLGPGLGVYLVKSDKHELSLEIGPSYVWE
                     KVDGATDDFLALRIAERGSCQITDNTKFVQSVEYVPNTEDFNDYLITAEAGVETAMSE
                     RWSLRVVLQDKYDSTPAADAEHNDISLIAGVAFTL"
     gene            5243..5911
                     /locus_tag="PKI64_07390"
     CDS             5243..5911
                     /locus_tag="PKI64_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
                     /protein_id="HOE00867.1"
                     /translation="MKPTGAQSSLHSAIHRLAGWALVWLLLAGCTPPPKPPPPKVVNT
                     SSELAAVAAPPVTRDPALRQVDKGPEDALLLMQVAAYRERLPEEFRKRNNFAWAQAHI
                     AGLEKTEYFAHSSIQDFLDFSTEAAEAMIGISLKPPEPARFTTLCVNQAGAVDGADCW
                     SRSVDTEFKILEDIASRLPDPSAKGHIRLFTELYPCPSCWNVMKQFLAIYTNVEMEVL
                     YRTP"
     gene            5925..6296
                     /locus_tag="PKI64_07395"
     CDS             5925..6296
                     /locus_tag="PKI64_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00868.1"
                     /translation="MTAHSPTPTSPGSYYELRCAVVEMFYETMLAEGYTIGQAASRCL
                     VEFRQEITPGGRDALVALSVILSRVAHHDAPALSRFAAEVARLKQLAAQNELRAGLSA
                     AEHSRLREDLSYLWERVPPAT"
     gene            complement(6730..7434)
                     /locus_tag="PKI64_07400"
     CDS             complement(6730..7434)
                     /locus_tag="PKI64_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00869.1"
                     /translation="MAYRFLCAAALVAVALAGVVGAAPAAEPRQDQDILKHAVVCPPF
                     KGDPTLAAVYHNELVKILRESPAVELYEGTRQLPDFYFRVNGSIVTNEDNQFFVLVTV
                     FDDERKEQLASHVAPATLDPEAILGWTRLLQADIARRGSRLPFECRISRKLGQESITL
                     DRGLGSGLKPGMVLYVSEDEEPLLSPVTGAIIGRDSPRAAGQIQVFRVLDETAYARPV
                     MNTKLPRTAKLFARSF"
     gene            complement(7456..7971)
                     /locus_tag="PKI64_07405"
     CDS             complement(7456..7971)
                     /locus_tag="PKI64_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00870.1"
                     /translation="MKKILLLACALALTAGAAMALEVTPLQIGVAGTKAQLFAAETRV
                     MGLRLNIACSDNDDVTGIDVGLVGLGRRMDALQLNLVNRVDAEFAGVSVGLFGEIGSL
                     AGIQAGLFNTVTHDMSGFQLGVFNTADSVMGIQVGLINRAVTLRGLQLGLINLVEEGP
                     LTFFPVLNFAF"
     gene            complement(7973..8560)
                     /gene="lpoB"
                     /locus_tag="PKI64_07410"
     CDS             complement(7973..8560)
                     /gene="lpoB"
                     /locus_tag="PKI64_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOE00871.1"
                     /translation="MKTTWTLAITGCLLALLATGCRTTVEYGDATSAKPISTDFGSAD
                     LQQIAAKMVDSLLADDVLADISAGGPPLLIVDKVKNKTMQHIDTESVTDSIRTKLIRS
                     RKFSFQDRTTEAALQEELAYQQAAAHDAIAFGQQDAPRYMLTANLSEIEQQQGRLKDV
                     YYKFTMSLRDLKSGRLIWADEQEIRKQKTRAIFGG"
     gene            complement(8608..9054)
                     /locus_tag="PKI64_07415"
     CDS             complement(8608..9054)
                     /locus_tag="PKI64_07415"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HOE00872.1"
                     /translation="MKSKWMTGIALAALVVLVAGCASPNTSGITVGAETDEYGNLQQV
                     LQVDNAKLAKQLLVQDVVVGQTKNGLMKASIKLTSRLNKDVVSQSKFAWFDADGNELD
                     PDTDPWRPLLLHGKETRTIQGVAPNAAAASFKLRVRAGEKTQWIIK"
     gene            complement(9073..10140)
                     /locus_tag="PKI64_07420"
     CDS             complement(9073..10140)
                     /locus_tag="PKI64_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOE00873.1"
                     /translation="MKKMWWIIALGLMAGVAAQAAPRAALFVQNQAGAKLENQLDAFN
                     DLVSTRLSAAGIETIRAQDVLARFTESRAAAQAQELRQAVEALQTLKAEGTVDGPLYE
                     AAALRTAQLMDADFLVFASLVSLGENQMTVQAYGLTQQQQITTLRVALRVLDGTLGTQ
                     LYGDTVAVSEKVLQNSNMQVQLGDLVNTLLDRGAEELAGRVKSQVARIEAAKAEAPAL
                     ASVTINSSAAGAAVELDGVVIGTAPGVFQIRPGVHEIRVTREGYATWEKSIALADGQV
                     LDVPMELSGAGIARKGELEAQDIAREQSAADAQATTTIAGGVAAQASNSYIRLEGMPG
                     QSLTIGDTDSSRGNVNVIQQQ"
     gene            complement(10163..11638)
                     /locus_tag="PKI64_07425"
     CDS             complement(10163..11638)
                     /locus_tag="PKI64_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00874.1"
                     /translation="MVRGRFIPVNLQIWRRVGLGLLLILGSAGCSTFSHYPTGMETTT
                     LAPLRAGKTPDYKKNFGPRLQGNTGVLFAMEMGRVAQVTGDADASREAFAKASALTRE
                     QDEKAVISASGAAAQGGAVLVNDKAIPYRAPSYERTLVHHYQALNYLTTNDLTGAGVE
                     VRLANREQEEARRKREAEINRAKSKRKNPEAAPEATADGEERDPHLDKVYAGLDQVAG
                     EVKSSFQNAATFYMSGVIWEMLGEQNDAYIDYKRALEIYPENPYLQADVVRLGKRLGM
                     REDVDDFARRFPAAADTPAAGTTAWADKARLVVLLDQGLVIPKEEISVAYPLGRTLSA
                     IALPAYTIAPPAPVPLVVKVDGKRAGTTAPICNISALAARALGEQMPGILTRQIARVV
                     AKTAAAEVASQQGGEIVGLLAAIYNVASEQADLRSWLTLPAHIQIASVWVTPGEKEVA
                     LAAPGDGTTWSGVVTLTAGKTTILYVTQLDYAVFSRIMMQP"
     gene            11663..12493
                     /locus_tag="PKI64_07430"
     CDS             11663..12493
                     /locus_tag="PKI64_07430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE00875.1"
                     /translation="MPRDTKKLVWCDAHCHLQDPRLAPHLAGVLRRAAESNVALLHVA
                     ATSEADWPAVRALAAAHPRRIIASYGLHPWYIANRTPRWLHRLREILAAAPAGIGEIG
                     LDGKLADGLDRDREAVFLAQLDLAYELGVPATIHCRDAWGPMLAILLNAPRHPAGLLI
                     HAYSGPPDVLRDLAARNIHISFGGTLTRPQNHRARENARRVPDGHYLLESDAPDLPPT
                     LPPGRSPHLLGDDSRTLSEPAYIPYTGAILAELRQTTPAEIADATTANAHRLFAKLLP
                     "
BASE COUNT         2382 a         4015 c         3679 g         2427 t
ORIGIN      
        1 ggttgatcca catcaccatg atgcatgaac gaaaccccat agcatctaca atcgaaccgg
       61 ctgcttttgt aggccgggaa cggctggaag gtctgcgacc gttccagcca caatcgaacc
      121 ggatgctttt gtggggcggg accggctgga acgtctgcgt ccgatggcgg gcgcgtcagt
      181 tgtcgagctg gaacgtgatg ctgatggttt tggaggtgcg atagcccaca ggcagcgcgg
      241 ggagtttctt gaccgaggtg atggcccgga tcaccgaatc atccatcagc gcattgccgg
      301 atccgcgcgt tttgctgtgg ccggtgatgg tgccatcggg ggcgacggtg atgttaacat
      361 cggtgctgag actgcccagc gacgccaact gcgccggctg ttgccacaca gcatagagca
      421 gctcgcgcac gcggttcaaa taggcggcaa aggcgatttg gtcatcggcg gggattttgg
      481 tttccgtgcc caccaccgcg ccttgtttga ggagccgcgc aatttcctcc ggggagagcg
      541 gcttgtcctt gggcggcggc gcgttgcgga ccacgcggtt ggtttggcgg atatccttgg
      601 gtggttcagg cggcttggga ggtttgggcg gggcgggagg cttgggtttt tccggatccg
      661 gcaacttgat gtcgtccgcc ggtttggggg gctcaggcgc cggcggttcg ggggtggggg
      721 gcgtctccgg ctcggggggc ggcggaatcg aaaccgtgaa ctccaccatc attacctgct
      781 ccggtttttt gggccggtgg caaccgcgaa aaatcagcgg ccacaggaga atgcccgcca
      841 gcaccacgct gtgcagcgtg aggctgagcc gcattccttg gcgcctgaac gagcgggggt
      901 tcatttcccg ggatcggtca ctatggccat gcgcgtaatc tgggcctcgt gcatcaaacg
      961 cagcacattg accaccttgc cgtaggccag ggtttcatcc gcgcgcacca ggacggtgat
     1021 ttccggattg tccgccacct tggttttgag tgcgttggcc agggcttcct ccgtgatggc
     1081 cgcattgtcc aaaaacagct ccgcattggc gttgagtgtg atggccaggc tgtcctgatg
     1141 ttccagcgtg gtggtcttgc ccttgggtag ccgcacggca atgccctgct cgatgtttgg
     1201 aaacgtgatc atgaacgtca gcagcagcag gaacgtgagg tccatgatgg acgtcatgtt
     1261 cagctcgctg atgaagcgcg tgttggtgag cgacgtgcgg cgggccatgg ccgattccta
     1321 ttcgtacacg aaggattgct gcaggcgcgt ggcgaacttc tccgtgaagt tttccaactg
     1381 gatgctcaaa aaccggattt ggtcattcag tttgttgcag ccgaatgccg agggaatcgc
     1441 caccaacaag cccacaatgg tcgtcagcag cgcgctggaa atgcccggcg ccgcttcgga
     1501 caaattggcc gagccctgct tggccatttg ctcgaacgat accgagatgc cccataccgt
     1561 gccgaacaag cccagcatcg gcgcgatggt gtaggtggaa cccagcatgg acatttgcgt
     1621 ttccagcagc aggatttgat cggccacggc gcattcggcc accttgcgga tggcatcaat
     1681 ctgcaacgca gtgagccgct cctgcgagag gtcaatctgg ctgagcggcc gctgctgttt
     1741 ttgcgcgcgg gtttcaaatt cccgcttcac ggccaggcag gcggcggcat acaccttggc
     1801 catcgggctg tcgtggtgga ccggcccgcg cacaaacatt tccagcgggt tgtgctgctg
     1861 gtcgaagtcg cgcatgaaat tggcgtccgc gcgtccgacc ttgcgcaact ccagatattt
     1921 tgccgccatg atgctccagg caaaaaccga ggcaaaaatc agcaccacca cgatgatttt
     1981 gccgaccggg tcggagatgt tgaacgccca caacacgccc gccagtggta atgctcccgt
     2041 caagaaactc acattcatat ttgcctcgcc aaaagaaggg tggaatttgg cgcgcatcat
     2101 acggcaaccc accggggggg tcaatcggcc tcgctgcagg gttgccgccg cgcgcgcggc
     2161 gtgggatact ccgcgctccg ttgcactatg gagatgacca atggctgaaa agaaacgcac
     2221 cgcggaaacc gccgaaagca tggccaagcg ccagcgcgag atttcggtct ccgaattttt
     2281 cctgaagaac cgccatctgc tcgggttcga cagcccccgc aaggccatca tgaccgccgt
     2341 caaggaggcc gtggacaact cgctggatgc ctgcgaggaa gccggcatcc tgcccgaaat
     2401 ctccgtggaa ttgcagcagc aggccgaaaa ccgctttgtg gtgatcatca ccgacaacgg
     2461 ccccgggatc gtgcgggcgc aaatccccaa agtgtttgcc aagctgctct acggctccaa
     2521 atttcaccgc ttgcggatgt cgcgcgggca acagggcatc ggcattagcg cggcggggat
     2581 gtacggccaa ctaaccaccg gcacggccac catcattctt tcgcgcacgc gcagcgccgg
     2641 caacgcccac cgactgtccg tgcagattga cacccgcgcc aacaagccgg ccgtgctggg
     2701 tgacgaagaa gtggactggc gtcccacctt cccccccgaa gcgcccggcc aagaaccggt
     2761 gacccatgcc cacggcacgc aggtgaccat cgaaatgact gcgcaatatg tgcgcggccg
     2821 gctctcggtg gatgaatatc tcaagcaatg cgcggtggcc aacccgcatc tgcggctgca
     2881 cttccgcgtc accacgctca agaaggacaa gagcggcgcg ttggcgccgg aaaccggcga
     2941 ctggcaaacc tacctgcgcg ctgtgcgcac gttgccgccg gtgaccaagg ccatccagcc
     3001 gcatccgcac ggcgtggagc tgggcgtgct gctgcaaatg ctcaaggata ccgcggcgcg
     3061 cacgttgaaa ggcgcgctgg aacaggagtt ttcgcgcgtg agcgcgcgcg ccgcgcagga
     3121 aatttgcgag cgcgccggac tcaatcccaa ggccaacccc acgcgcgtgg cccatcacga
     3181 aatcgaggcg ctcttcaagg ccattcagga aaccaaactg atgcgcccgc ccaccgactg
     3241 tctcgcgcct atcggcgagg aacaaattct ggcgggattg aaaaaggagt tccccgccga
     3301 tttttatgct gccgtcacgc gcgcgccgga agtatatcgc ggcaatccgt ttcaggtgga
     3361 agtgggcgtg gcctatgcca agcccggcac caacgcggaa ctgggcgcgg aagaaccggt
     3421 gcgcgtcatg cgttttgcca accgcgcgcc gctgctctac atgggcggcg cctgcgccat
     3481 gaccgaggcg ctcgaaaatg tgaactggaa agcctacggc ctgttgcagc ccaagggcag
     3541 cctgccgctg ggacccgtgg tgttcttcct gcatatcgcc agcgtgtggg tgcccttcac
     3601 cagcgaaagc aaggaagccg ttgcgcacta cccggaaatc ttgcgcgaaa tgacctttgc
     3661 gcttcaagag gcggggcggc aactggccgt gtacttgagt cggcgccgcc ggcaggccga
     3721 agccgaccgc aaacggggct acatcgaaaa atatattccc cacctcgcgc tcggcctgca
     3781 agaaatcctg accctctccg acaagcaaaa aaccaaggtt gaagacgacc tcacgcggat
     3841 gctcgaacgc acccatctgg aaacatgatc cagcgcgcga gttgacaaac cggcgcccgt
     3901 gtgtaacgtt ttacgcgcaa taaaacgcaa ccaaggagaa cataacatga gtatcatcaa
     3961 ggaattccaa gagtttgccg tcaagggcaa catggtggat atggctgtcg gtatcattat
     4021 cggcggtgca ttcggcaaga ttgtcacctc gctggtgaac gatgtcatca tgcccccgct
     4081 cggcatgctg ttgggcaagg tggatttcaa ggacttggcc attacgctca aggaagcggg
     4141 cgtggatgcc gccggcgcgc cggtggaagc cgtcaagctc agctacggcc tgttcattca
     4201 gaataccgtg gattttctga tcgtggccat cgccgtgttc atgatggtca aggccatcaa
     4261 taccctgcgc cgcaagcagg aagccgccag ctaacattca actgcaagaa agaaaaccca
     4321 tgaagaagtt ttggattttg ttgatcgccg ccagcttggc caccgccgcc tcggccgcgg
     4381 aaaagaaagc cgccaaagcc gctgaaaagc cgaccgccaa acccgccttt gccaccacgc
     4441 tgaacgcggg attgaccatg acggatggca acagcgagac gctcgcggcc aatgccagcc
     4501 tcgtcaccga aggcgaaaaa gagggtctcg gctccgttcg cgccggcgtg gaaggcaact
     4561 acggcgaaag caccgtgaac gatgaaaagg aaaccacggt ggaaaacgcc aaggtgttcg
     4621 ccaacgtgaa gaagacgttg accccccgca ccttcgcggt gctggatggt tccgtgttgt
     4681 atgacgacgt ggcgctcgtg gattaccgcg tgaccctcgg ccccggcctc ggcgtgtatc
     4741 tggtcaagag cgataaacac gaactttcgc tggaaatcgg cccctcctac gtctgggaaa
     4801 aagtggacgg cgcgacggat gacttcctcg cgctgcgcat tgcggagcgc ggctcctgcc
     4861 agattacgga caacaccaag tttgtgcagt cggtcgaata cgtccccaac accgaggatt
     4921 tcaacgacta tctgatcacc gcggaagccg gcgtggaaac cgccatgagc gagcgctgga
     4981 gcctgcgcgt ggtcctgcag gacaagtacg acagcactcc ggccgccgat gccgaacaca
     5041 acgacatcag cctgattgcc ggcgtggcgt tcacgctgta accacaaacc ggcttggccg
     5101 acgttgtgcc aacgcccgcg caatcccgcg cgggcgtttt gcttggctgc atcgtgccag
     5161 gggggggcac gaaaacgttt ttgcgtggcg atcagaatag gcttggggcg cgcgggcccc
     5221 tggcctagcc ttgtcgaacc tcatgaaacc aaccggagcc cagagcagct tgcattccgc
     5281 catccatcgg ctggccggct gggcgctggt gtggctgctt ttggcgggct gcacgccccc
     5341 gcccaagcca ccgccgccca aagtggtcaa cacgtcgtcc gagttggcgg cagttgccgc
     5401 gccgccggtc acacgcgacc cggcgctgcg ccaggtggac aaaggtccgg aggatgcgct
     5461 cctgctcatg caggtggcgg cctaccgcga acgcttgccc gaggagttcc gcaagcgcaa
     5521 caattttgct tgggcgcagg cgcacattgc ggggctggaa aaaacggaat attttgccca
     5581 cagcagcatc caggatttct tggatttctc caccgaggcc gccgaggcga tgatcggcat
     5641 ttccctcaaa cctcctgaac cggcgcgctt caccacgttg tgcgtgaatc aagccggcgc
     5701 ggtggatggc gctgattgtt ggtcgcgcag cgtggatacc gagttcaaga ttttggagga
     5761 tatcgcctcg cgactgccgg acccctctgc gaaggggcac atccggctgt tcacggaact
     5821 ttatccgtgt cccagttgct ggaacgtgat gaagcaattt ttagccatct acaccaatgt
     5881 ggagatggag gtactatacc gcaccccgta agacgacctg catcatgaca gcccactccc
     5941 caactccgac ttcgcccggc agctattacg aactgcgctg tgccgtggtg gaaatgtttt
     6001 atgaaaccat gctggccgag ggctacacca ttggtcaggc cgccagccgc tgtttggtgg
     6061 agtttcggca ggaaatcacg cccggcggcc gggatgcact ggtggcgttg agcgtgattc
     6121 tgtcgcgcgt ggcgcatcac gatgcgcccg cgttgtcgcg gtttgctgcc gaggtggcgc
     6181 ggctcaagca gttggccgca caaaatgaat tgcgcgccgg cttgtccgcc gcagaacact
     6241 cccgcctgcg cgaagacctc agctatctct gggaacgagt gccacccgca acataatccc
     6301 cagcgtggga tcgtcgcctt ttttttgcgg gggtattggg agcggcaacc gccacacgag
     6361 accgcacctc gcggataaag atgcaaaaac cactgacctt aaaaatgtcc agagtttata
     6421 aactgtggac aatggtcaaa aaatggtgga tcataaaacg cctaagaaat agatgtaata
     6481 aattgtgtat gagtaagata taaatattac gaaatatgcc cagagtttat aaagtctgga
     6541 cagtgtggtt caaatggaaa aaagaaggta gcctgacaac tgctgactcc acatcccaag
     6601 caggacgatc cagtcactga aaacagcgcg gaaccccacc gcacgggggg ggggcttgat
     6661 ccatttacac gacgcacccc gcggcgtgga gccgtggggc gcgtacgggc cgggctgtgg
     6721 ccggcggcat caaaaactgc gcgcgaagag cttggcggta cgtggcagct tggtgttcat
     6781 cacgggccgg gcgtaagcgg tttcgtccaa cacgcggaat acctggattt gtcccgcggc
     6841 gcgcggcgag tcccggccaa taattgcccc ggtgaccggc gaaagcagcg gttcttcgtc
     6901 ttccgagaca tacaacacca tgccgggctt caagccagag ccaaggccgc ggtccagcgt
     6961 gatggattcc tgcccgagct tgcggctgat ccggcactca aacggcagcc gcgacccgcg
     7021 ccgcgcaata tccgcttgca aaagacgcgt ccagccgagg atggcttcgg gatcaagcgt
     7081 ggccggagcc acgtgcgaag ccagttgctc cttgcgctcg tcatcgaaca ccgtcaccag
     7141 cacgaagaat tgattatcct cattggtgac gatggaacca ttgacgcgga agtagaaatc
     7201 cggcaattgc cgggttcctt catacagctc cacggcgggg gactcccgca ggatcttgac
     7261 cagctcgtta tgatacaccg cggccaaagt cgggtcgccc ttgaacggcg gacagacgac
     7321 cgcgtgcttc aagatgtcct gatcctgccg gggttcggcg gccggtgcag ctccgaccac
     7381 gccggccaag gccacggcca ccagtgccgc cgcgcataaa aaccgatatg ccatgcgata
     7441 tcctttcgtc tgccgctaaa acgcgaagtt gagcaccggg aagaacgtca acgggccttc
     7501 ttccaccaga ttgatcaagc ccagttgcag gccgcgcagt gtcaccgcgc ggttgatcaa
     7561 cccgacctga atgcccatga cgctgtcggc cgtgttgaac acgccgagct ggaagccgga
     7621 catgtcgtgg gtcacagtgt tgaacagccc ggcttgaatg ccggccagcg agccgatttc
     7681 accgaacagg ccgacactca cgccggcaaa ctcggcatcc acccggttca caaggttgag
     7741 ctgcaaggca tccatccgcc ggcccagccc cactaggccc acatcaatgc cggtgacgtc
     7801 gtcattgtcg ctgcacgcaa tgttcaaccg caggcccatc acgcgggtct cggcggcgaa
     7861 cagttgcgcc ttggtgccgg ccacgccgat ttggagcggc gtgacttcca gcgccattgc
     7921 cgcgccggcc gtgagggcca gcgcgcaggc cagaagcagg atttttttca tgtcagcctc
     7981 cgaagatcgc gcgggtcttt tgcttgcgga tttcctgttc atcggcccag atcaggcggc
     8041 cgctcttcag gtcgcgcagg ctcatggtga acttgtaata gacatctttc aggcggccct
     8101 gctgctgctc gatttccgac aggttggcag tcagcatgta gcgcggggca tcctgctggc
     8161 caaaggcaat cgcgtcgtgg gctgccgcct gctgataggc gagctcttcc tgcagcgcgg
     8221 cttccgtggt gcggtcctgg aagctgaact tgcgcgagcg gatgagtttg gtgcggatgc
     8281 tgtccgtgac cgactcagtg tcaatgtgct gcatggtctt gttcttgacc ttgtccacaa
     8341 tcagcagcgg cgggccgccg gcggagatgt cggccagcac gtcatcggcc agcagcgagt
     8401 ccaccatctt ggcggcgatc tgctgcaagt ccgccgagcc aaagtccgtg ctgatgggct
     8461 tggcggaggt ggcatcgcca tattccaccg tggtgcggca gccggtcgcc agcagcgcca
     8521 gcaaacaacc cgtaatcgct aatgtccatg tagttttcat tttcttttcc ctcggcttgc
     8581 tgtggttcat tcgtgttttg acttcggtta tttgatgatc cattgcgtct tttcgccggc
     8641 gcgcacgcgc agtttgaagc tggccgcggc ggcgttgggc gccacaccct ggatggtgcg
     8701 tgtttccttg ccgtgcagca gcagcggccg ccacggatcg gtgtccgggt ccagctcatt
     8761 gccgtccgcg tcgaaccagg caaacttcga ctgcgagacc acatccttgt tcagccgcga
     8821 ggtcagcttg atgctggctt tcatcaggcc gtttttggtt tggcccacga ccacatcctg
     8881 caccagaagc tgcttggcca gcttcgcgtt atccacctgc aacacctgtt gcaagttgcc
     8941 atattcatcc gtttccgcgc ccacggtgat gcctgacgta ttgggcgagg cgcaccccgc
     9001 caccaaaacc accagcgccg ccaacgcgat cccagtcatc cacttgcttt tcatgtgtgt
     9061 ctccttccgc ctctattgtt gttgaatcac attcacattc ccgcgcgagg aatccgtgtc
     9121 gccaatggtg agcgactgtc ccggcatacc ttccaggcgg atataactgt tgctggcctg
     9181 cgccgcgaca ccgccggcaa tggtcgtggt ggcctgtgca tccgccgcgc tttgttcgcg
     9241 cgcgatgtcc tgcgcttcca gttcgccctt gcgcgcgatg ccggcgccgg aaagctccat
     9301 cggcacatcc aacacttggc catccgccag cgcgatgctc ttttcccacg tggcatagcc
     9361 ttcgcgcgtc acgcggattt catgcacgcc cggccggatt tggaaaacgc ccggcgcggt
     9421 gccgatgacc acgccatcca gttccactgc cgcgcccgct gccgaagaat tgatcgtcac
     9481 gctggccagc gccggcgctt cggctttggc ggcttcgatc cgcgccacct ggctcttcac
     9541 ccgaccggcc agctcttccg cgccgcggtc cagcaacgtg ttcacgaggt cgccaagttg
     9601 cacctgcata ttggagtttt gcaggacttt ttcgctcacc gccactgtgt cgccataaag
     9661 ctgcgtgccg agggtgccgt ccagcacgcg caacgccacc cgcagggtgg tgatctgttg
     9721 ctgctgggtc aggccgtacg cctgcacggt catttggttt tcacccagcg aaacaagcga
     9781 cgcaaacacg aggaaatccg cgtccatcag ttgcgcggtg cgcagcgccg ccgcttcata
     9841 cagcgggcca tccaccgtgc cctcggcctt caatgtctgc agcgcctcga ccgcctgccg
     9901 cagctcctgc gcttgcgccg ccgcgcgcga ttccgtaaag cgcgccagca cgtcctgcgc
     9961 ccggatggtc tcaatcccgg cggccgatag ccgtgtgctg accagatcgt tgaatgcgtc
    10021 caattgattt tccagcttgg cgccggcttg gttttgcacg aacaatgccg cacgcggcgc
    10081 cgcctgtgcc gccactcccg ccatcaaccc cagcgcgatg atccaccaca tttttttcat
    10141 gtttgcgctc ctttagtgtt cctcatggtt gcatcattat tcttgaaaag acagcataat
    10201 ccaattgcgt cacataaaga atagtcgttt tgcctgccgt caacgttacc acacccgacc
    10261 acgttgtgcc gtcgccgggc gcggccaggg cgacttcttt ttcgccgggg gtcacccaca
    10321 cgctggcaat ctggatgtgc gccggcaggg tcagccagct ccgcaggtct gcctgttcag
    10381 acgcgacgtt gtaaatcgcc gccagcaacc cgacgatttc gccaccctgt tggctggcca
    10441 cctctgccgc cgcagttttg gccaccacgc gcgcgatttg ccgggtcagg ataccgggca
    10501 tctgctcgcc caaagctcgc gccgccagcg ccgagatatt gcagatgggc gcggtggtcc
    10561 ccgcccgctt tccgtcaacc ttcaccacca gcggcacggg ggccgggggg gcaatggtgt
    10621 aggcgggcag ggcaatggcg ctcagcgttc tgcccagcgg ataggccacc gaaatttcct
    10681 ctttgggaat caccaagccc tgatccagca gcaccactag tcgcgctttg tcagcccatg
    10741 ccgtggtgcc ggctgccggc gtatctgccg ccgccggaaa gcgtcgcgcg aagtcgtcca
    10801 catcctcgcg cattcccaac cgcttgccca gccgcaccac atccgcctgc aaataggggt
    10861 tttcaggata gatctccagc gcgcgcttat aatcaatata ggcgtcattc tgctcgccca
    10921 gcatttccca gatcacgccc gacatgtaga acgtcgccgc gttttggaac gacgacttca
    10981 cctcgccagc cacctggtcg agccccgcat acaccttgtc caaatgcgga tcgcgctctt
    11041 cgccatcggc ggtcgcttcc ggcgcggcct cgggattctt tcgcttggat ttggcgcgat
    11101 tgatttcggc ctcgcgctta cgccgcgctt cctcctgttc gcgattggcc agccgcacct
    11161 ccacgcccgc gcccgtcagg tcgttcgtcg tcagataatt cagcgcctga taatgatgca
    11221 ccagcgtgcg ctcgtaactg ggtgcgcggt aggggatggc cttgtcgttc accagcaccg
    11281 cgccgccctg cgccgccgca cccgatgccg aaatcaccgc cttctcatct tgctcgcgtg
    11341 tcagcgcgct ggctttggcg aatgcctcgc gcgacgcatc ggcatcgccc gtcacctgcg
    11401 ccacgcggcc catctccatc gcgaaaagca cccccgtgtt gccctgcaac cgcgggccga
    11461 agtttttttt gtaatccggc gtttttcccg cgcgtagcgg cgccagcgtc gtcgtctcca
    11521 tgccggtggg ataatggctg aaagtgctgc atcccgcgct gcccagaatc agcagcagac
    11581 ccaaccctac ccgccgccag atttgcagat tgactggaat gaaccggcct ctcaccatgt
    11641 cccgtggaaa ataccatgcc ttatgccgcg cgacactaaa aaattggttt ggtgcgatgc
    11701 ccactgccac ctgcaggacc cgcgccttgc gccgcacctg gcgggcgtgc tccgccgcgc
    11761 cgcggagagc aatgttgccc ttctgcacgt cgccgccacc agcgaggccg actggcccgc
    11821 cgtccgcgcc ttggccgcgg cccatccgcg ccgcatcatt gcctcgtacg gcctgcaccc
    11881 gtggtacatc gccaaccgca ccccgcgatg gctccaccgc ctgcgcgaaa tcctcgccgc
    11941 cgcccccgcc ggcattggcg aaatcgggct ggatggcaaa ctcgccgacg gcctcgatcg
    12001 cgaccgcgaa gccgtcttcc ttgcccaact cgacctcgcc tatgaactcg gcgtgcccgc
    12061 caccatccat tgccgtgatg cctggggccc gatgctggcc attctcctca atgccccacg
    12121 ccaccccgcc ggcctcctca tccacgctta ttccggcccc cccgacgtcc tgcgcgacct
    12181 cgccgcgcgc aatatccaca tttcgtttgg tggcaccctg actcggcccc aaaaccaccg
    12241 cgcccgcgaa aacgcccgcc gcgtccccga cggccattac ctcttggaat ccgacgcccc
    12301 cgacctgccg ccgaccctgc cccccgggcg ctctccgcat ttgcttggcg atgattcccg
    12361 taccctctcg gagcccgcct atattcctta cactggcgct atcttggccg aactgcgtca
    12421 aaccactcct gccgagattg ccgacgccac cacggccaac gcccatcgct tgttcgccaa
    12481 gctgctgccc taacaccgcc ata
//
LOCUS       DAOPNP010000071        12413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_5917_length_12413_cov_9.290824, whole genome shotgun sequence.
ACCESSION   DAOPNP010000071 DAOPNP010000000
VERSION     DAOPNP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5917_length_12413_cov_9.290824"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKI64_07435"
     CDS             <1..293
                     /locus_tag="PKI64_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE00876.1"
                     /translation="IFDMRWVVTGSYNFTTTAQVSNWENLVWVDSPDMAEQYKQAWDA
                     IVSDEVVEPRAEKTAAPKNPRPPKRRNIDGGAGAGLVFREDWRHADPGTEPA"
     gene            290..1177
                     /locus_tag="PKI64_07440"
     CDS             290..1177
                     /locus_tag="PKI64_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOE00877.1"
                     /translation="MSLMSMTGRGGAAASCRLAHVEVELSSVNRKQLDMDISLPRSLA
                     LFESRIQEQVRARIARGRISGEVRVTWSAAAQAAGVRVDQGLARASVAALRAAAKTLG
                     LPDDLQASALLAVPGVIVQERRAQDIEALWPALRRALAEALTQLLAMRRKEGAALQRD
                     LRRRLATLRRLARQIAARAPAVAAAYRESLRQRIAAAVPGSDLAADDRLLKEVALFAD
                     RADITEELVRLQSHFAQTAEALQQGGVVGRTLDFLVVEMGREINTIGAKANDAAIARL
                     VVAGKTELERVREQVQNIE"
     gene            1192..1833
                     /gene="gmk"
                     /locus_tag="PKI64_07445"
     CDS             1192..1833
                     /gene="gmk"
                     /locus_tag="PKI64_07445"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOE00878.1"
                     /translation="MNGIKPCWKNIIFVVSAPSGAGKTTLCDKLQKEFKDIHYAITAT
                     TRAPRGDEEDGKSYYFMTMPDFEKKLAEGGFIESAVVHGNRYGSPKGPVVAALEDGCD
                     VLMNLDVQGAAAVRNYIQNAAPGDPLKRTLVDIFVVPPSAEVLRNRLVARGTDALEII
                     EKRMRQAREEISHWKEYKYMVVNDDLAEAYDNMRAIILAERHRNDNRREDYHG"
     gene            1826..2377
                     /locus_tag="PKI64_07450"
     CDS             1826..2377
                     /locus_tag="PKI64_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOE00879.1"
                     /translation="MAEAPKKIVLGVTGSISAYKSAELARLMKKRGWDVWMVLTSCAT
                     QYVGPLMLRALTGNPVTCGSFAEATLDTYSHLALAGGAAAMVVAPCSAQTIARLAHGM
                     ADDLLSCTALALTAPLLVAPAMNTHMWQNAAVQENVATLQRRGVIIIPPAEGELACGE
                     IGPGRLNELPVIMAAVARAVGET"
     gene            2382..3047
                     /locus_tag="PKI64_07455"
     CDS             2382..3047
                     /locus_tag="PKI64_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOE00880.1"
                     /translation="MNVLVTAGPTREAIDPVRFVSNRSSGKMGYAIAAAARRAGHTVC
                     LISGPVARPAPRGVEVVRVTTAAEMLAAVQRRVRSCDLLVMAAAVADWRPKQPARRKL
                     KKQDGPPRITWEPTPDILQTVAQRKTARQIFCGFAAETHDLAREAQRKLRAKNLDAIA
                     ANDVARADRGFEADHNALTIFFADGCVRQVGWTTKARAARVLLRELAAYADRKKSENI
                     FEK"
     gene            3266..3388
                     /locus_tag="PKI64_07460"
     CDS             3266..3388
                     /locus_tag="PKI64_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00881.1"
                     /translation="MAVKKAKKAAPKKAAKKAAPKKAAKKAAPKKAAKKVAKKK"
     gene            3568..3795
                     /locus_tag="PKI64_07465"
     CDS             3568..3795
                     /locus_tag="PKI64_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOE00882.1"
                     /translation="MQEKVEQFIEEIRPLIQGHGGDVEFVSMEGNTVHLRLHGACHSC
                     PHAMMTLKNGIEAEMRDRLGPELTVERVMDA"
     gene            complement(3875..4816)
                     /locus_tag="PKI64_07470"
     CDS             complement(3875..4816)
                     /locus_tag="PKI64_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE00883.1"
                     /translation="MNMRKVCRATYQARNGPQDKSSTIFPAAGRFCFPSRVGSVILWS
                     MMDTAIPALPDRLRLDRPLVFFDIEATGLNKRTDRIVAIALVRYEPSGTAQKISYLLN
                     PGVPIPEDATAIHGITDADVEGAPTFADMAEILLDHFAGADLAGYNILGYDIQILTEE
                     FARAGHPFPLEGRRILDAQRIFFRNEPRDLAAALRYYCADDHTDSHDALGDVLATIRV
                     LGGQFQKYAELPADLDGLHEYCDPRDPSWVDRTGRLKWSRGEIVFNFGKVQGQPLREV
                     VAKDPNFLTWLLRSDFPEDTKQIVRNAQAGHYPAPPA"
     gene            4881..5135
                     /locus_tag="PKI64_07475"
     CDS             4881..5135
                     /locus_tag="PKI64_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOE00884.1"
                     /translation="MKIYVGNLSFKATDDDLRAAFEPYGEVVSARVIKDKETGRSRGF
                     GFVEMPNQEQGNAAISKVNNTDIVDRPVRVNEAQERPPRR"
     gene            complement(5220..5471)
                     /locus_tag="PKI64_07480"
     CDS             complement(5220..5471)
                     /locus_tag="PKI64_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE00885.1"
                     /translation="MAYNIQYKRSVAKDLARLDKAAARQLLAKIEKELATRPGRFPAL
                     VGPFAGLRKMRMGDYRVIFTLLDKEVLVLRIGNRRDVYK"
     gene            complement(5461..5679)
                     /locus_tag="PKI64_07485"
     CDS             complement(5461..5679)
                     /locus_tag="PKI64_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOE00886.1"
                     /translation="MSAILSIRVPVPLAEELNQIAEETERSRSFHIQKALENYIAEYA
                     DLQIALDRLRNPRDPAVSGNELRKSLGL"
     gene            complement(5795..6631)
                     /gene="rsmA"
                     /locus_tag="PKI64_07490"
     CDS             complement(5795..6631)
                     /gene="rsmA"
                     /locus_tag="PKI64_07490"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOE00887.1"
                     /translation="MIEPPILTRPSEVRALLAQLDFHPSRILGQNFLIDRNILGILLA
                     AAELQPTDRVVEVGPGLGVLTDELLMRASAVSAVEKDHRLAAYLRTRFAEEPRLVLHE
                     GDILEADLPALFAAPGHKLVANLPYAIAARLLVELGALPHPPELIVVTIQREVAERLV
                     ARPGGKDYGLLAILLQRHYEIAVVKNISPSCFWPPPEVQSAIVKLTRRAAPLGGAANE
                     QALRELLRHAFSQRRKTLARSLRGVVVDPLPALAQVGIAPTARAEEIPPDRWPPLLRA
                     LR"
     gene            6691..7182
                     /locus_tag="PKI64_07495"
     CDS             6691..7182
                     /locus_tag="PKI64_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOE00888.1"
                     /translation="MRDAEQKIWTREALAGECTRLRTAGRKIVFTNGAFDLLHAGHAT
                     YLQFARRQGDVLIVALNTDASIRRYKGEKRPIVDGPNRARLMAALECVDYVVFFDEDE
                     PKALIAELQPDVLVKAEDWAHYVSGRDIVEARGGKVVLAPMVAGLSTTKLIEKILQAY
                     GAG"
     gene            complement(7167..8858)
                     /locus_tag="PKI64_07500"
     CDS             complement(7167..8858)
                     /locus_tag="PKI64_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOE00889.1"
                     /translation="MIPRPAHFSTLWKTFFHTVEKFLKVFPLCGKIRESFSIVWKSLV
                     AVALFSGTAAWASPFAVSAALTTHEQQPAVRVDFTVPEGHHLFESFTVQDTAGTALTP
                     LFVPPPDTNNPDDIEPSYGTSFTAFYKPPAGDRIVVGFQGCKGELCYLPEEVTLSLGA
                     ATAEESAPAIAASTTDWPDLLAQRGAPRRLVGYADAPEFLAFLDPAAAPPAPASAWRL
                     FLDDPAQFYLRRGLLPVLLLILVGGILLNLTPCVLPMIPINLAIIGAGGADTPRAMRF
                     GLGLVYGLGMALVYGALGLVVVLTGSVFGAINSSPVFNLAIAVLFVALALAMFDVWQL
                     DFSRFRKTGGVPGRIRLPAVLVMGGVSALLAGACVAPVLIAVLALAGSLYGQGIAAAL
                     ALPFLLGVGMALPWPLAAAGLAVLPKPGAWMEKVKKAFGVLILLLAAYYGWNAYTALR
                     AVPAVPEGAAPTTGGTALRSFDFKSGTSEEFAALLGEAVASGKPILLDFGAHWCKVCK
                     LMDATTLRDADVVAKLADYAAIQILADEPNKPPARDALAPFNIQGYPTFLLYPAP"
     gene            complement(8855..9460)
                     /locus_tag="PKI64_07505"
     CDS             complement(8855..9460)
                     /locus_tag="PKI64_07505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE00890.1"
                     /translation="MKTTQPVLLNNAAPAPRGRDPLVRLLLAGALGLLLASCSCRPVP
                     PAPVPIPPIHDFTLKNLASEFPGEIETVHYAGQVQLIIFLLTDDPGCRATLAEWNRLQ
                     QGYAAAGFTVIGVVADDRPLAAITAEAAELAPEFPLGLADVPVVTAFGGLSAIRAVPT
                     LFLLDRAGNLAHTYTGFVPMDTLRDDIAALLAAPAPSEDEP"
     gene            complement(9457..10632)
                     /locus_tag="PKI64_07510"
     CDS             complement(9457..10632)
                     /locus_tag="PKI64_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOE00891.1"
                     /translation="MTFTLYLLIGLAVGLTLGYLLAHFRAVAQVAQARAETIRAQTQV
                     EMLEAARTKLTDEFQRLSLQILGEQSAKFTQQQRAALSELLGPLDEKIKDFKQKVEHL
                     HSEESQQRSALRQQVELLATQSQAVSQQTGQLATALRGDNKTLGNWGELTLERLLDAS
                     GLEKGRDYQTQVSATSEGGQRQQPDVLINLPDNRCVIIDAKTSLKDYLAHVNATDEPA
                     RTATLKAHAVAFDTHINNLAKRRYQDLFPDRSPDFVLMFVPSEPALALALYARPALYE
                     TAAKANIILTGPGGLLATLRLVAQLWRQENQRQAITSIFDTIRKIYEKYVNFAEDMQQ
                     IDDALAKARNAYENAFKKLSTGDGNLLRQMENFRNQNIISPKKLPPPRFQVGGESEA"
     gene            10919..11569
                     /locus_tag="PKI64_07515"
     CDS             10919..11569
                     /locus_tag="PKI64_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00892.1"
                     /translation="MNTIKSLFGGSNGSARRDARTYVVDAARLVDEKTGRRLAPREQV
                     QLLNALARVAKQENLVFHAVFESERPLREVEHGGEFNGVKVYFAADSEKLVEQALKLC
                     KSEGSALISSGATLESRATEEGIAVLCSSTFRKAFLPSGLPSNERNGRGDRGSRERRR
                     DRGPRRERNDQRPPRPEAAPGASAGDNAAPDTGANPDPAPPQEDNSAVRNLIDLVE"
     gene            12076..>12413
                     /locus_tag="PKI64_07520"
     CDS             12076..>12413
                     /locus_tag="PKI64_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00893.1"
                     /translation="MDAFRIEKAVTRNNAHLITTRHHFYKIGIGSTNTVRLEYNKYRQ
                     IALHCQELTSHLPQVSFSTQCRRPVLQMKRIPQTDMTDIQHPQAARVLDILKRYSHPG
                     IPADWEAFPYL"
BASE COUNT         2523 a         3718 c         3966 g         2206 t
ORIGIN      
        1 tgattttcga catgcgctgg gtcgtcaccg gcagctacaa cttcaccacc accgcacaag
       61 tgtccaactg ggaaaatctg gtgtgggtgg attcgcccga catggccgag caatacaagc
      121 aggcgtggga tgccatcgtc tccgatgaag tcgtcgagcc gcgcgcggaa aaaaccgccg
      181 cgccaaaaaa tccaaggccg ccaaaacggc ggaacattga cggcggcgcg ggggcgggtt
      241 tagtattccg ggaggattgg cggcacgccg atccaggaac ggaaccagca tgagtttgat
      301 gagcatgacc ggccgcggcg gggctgcggc atcttgccgc ctcgcccacg tggaggtgga
      361 gctgagcagc gtcaaccgca agcaactgga catggacatc agcttgccgc gcagcctggc
      421 tctgttcgaa tcgcgcattc aagagcaggt gcgcgcgcgg atcgcgcgcg gtcggatctc
      481 gggcgaagtg cgcgtgacgt ggagcgccgc cgcgcaagcc gccggcgtgc gggtggacca
      541 agggttggcc cgggccagcg tggccgccct gcgcgccgcc gccaaaacgc tcggcctgcc
      601 ggatgatttg caagcctccg ccttgctggc ggttcccggc gtgattgtgc aggagcgccg
      661 cgcgcaggat atcgaggcgc tatggcccgc gctgcggcgc gcgctggcgg aagccttgac
      721 ccaactgctg gccatgcgcc gcaaggaggg cgccgccctg cagcgggatt tgcgccggcg
      781 gctggccacg ctgcggcggc tggcccgcca gattgccgcg cgcgcgccgg cagtggcggc
      841 ggcctaccgc gaaagtttgc gccagcgcat cgctgccgct gtccccggca gcgaccttgc
      901 cgccgatgac cgcctgctga aagaagtggc cctgttcgcc gaccgggcgg acatcaccga
      961 agaattggtg cgcctgcaaa gccattttgc gcaaacggcg gaagcgctgc aacaaggcgg
     1021 tgtcgtgggc cgcacgctgg attttctggt tgtggaaatg gggcgcgaaa tcaacaccat
     1081 cggcgccaag gccaacgatg ccgccatcgc gcggctcgta gtggctggca agacggagct
     1141 ggaacgcgtc cgcgaacaag tgcagaatat cgaatagaac tcggaggaga catgaacgga
     1201 atcaagccct gctggaaaaa cattattttt gtggtgtccg cgccctcggg cgccggcaaa
     1261 acgaccttgt gcgacaaatt gcaaaaggaa ttcaaggata tccattacgc catcaccgcc
     1321 accacgcgcg cgccgcgggg cgacgaagag gacggcaaga gctactattt catgactatg
     1381 cccgatttcg agaagaaact cgccgaaggt ggttttatcg agagcgccgt ggtgcatggc
     1441 aatcgctacg gctcgcccaa ggggcccgtg gtggccgcgc ttgaagatgg ttgtgatgtg
     1501 ctgatgaacc tggatgtgca gggcgccgcc gccgtgcgca actatatcca gaatgccgcc
     1561 cccggcgatc ccctcaagcg cacgctcgtg gatatttttg tggtgccgcc ctccgccgag
     1621 gtgctgcgca accgcctcgt ggcccgcggc accgatgccc tggaaatcat cgaaaaacgc
     1681 atgcgccagg cccgtgaaga aatttcccac tggaaggaat acaagtacat ggtggtgaac
     1741 gacgacctcg ccgaggctta cgacaacatg cgcgccatca ttttggcgga gcgccaccgc
     1801 aatgacaacc ggcgggagga ttaccatggc tgaggccccg aaaaaaatcg tgctgggcgt
     1861 caccggctcc atttcggcgt acaaatccgc cgagctggcc cgactgatga agaagcgcgg
     1921 ctgggatgtc tggatggtgc tgacctcgtg cgccacgcag tatgtcggcc cgctgatgct
     1981 gcgcgcgctg acgggcaacc ccgtcacctg cggctccttc gcggaagcca cgctcgacac
     2041 ctattcgcat ctggcgctgg ccggcggcgc ggcggccatg gttgtggcgc cgtgctcggc
     2101 gcagaccatt gcgcggctgg cgcacggcat ggccgatgac ttgctcagtt gcaccgcgct
     2161 ggccctgacg gcgccgctgc tggtggcgcc ggccatgaat acgcacatgt ggcagaacgc
     2221 ggccgtgcag gaaaacgtgg caacgctcca gcggcgcggg gtcatcatca tcccccccgc
     2281 ggaaggcgaa ctggcctgtg gcgaaatcgg tcccggccgg ctcaatgaac tgccggtcat
     2341 catggcggcc gtggcgcgcg cggtggggga aacctgaacg cgtgaacgtg ctggtgacag
     2401 cggggccgac gcgggaagcc attgatcctg tccgcttcgt cagcaaccgc tccagcggca
     2461 aaatgggcta cgccattgct gctgccgcgc gccgcgccgg gcacacggtc tgtttgatct
     2521 ccggccccgt ggcccggccc gcgccgcgcg gcgtggaagt cgtgcgcgtg accaccgctg
     2581 ccgaaatgct cgccgccgtg caacgtcgag tccgttcctg cgacctcctc gtgatggccg
     2641 cggcggtggc tgactggcgg cccaaacaac ccgcgcgccg caagttgaaa aaacaggatg
     2701 gcccgccgcg catcacgtgg gaacccacgc cggatatttt gcagaccgtt gcacaacgca
     2761 aaaccgcgcg ccaaattttt tgcggcttcg cggcggagac gcacgacctc gcgcgcgagg
     2821 cgcaacgcaa gctgcgcgcg aaaaatctgg atgccatcgc cgccaacgat gtcgcgcgcg
     2881 ccgaccgcgg attcgaagcg gatcacaacg cgctgacgat tttttttgcg gatggctgcg
     2941 tgcgccaagt gggatggacc accaaagcgc gcgccgcacg ggtgctgttg cgcgaactcg
     3001 ccgcgtatgc cgaccgaaaa aaaagtgaaa atattttcga aaagtagttg acgctgttaa
     3061 taacttcgca ttactgtgtt gttcaatgag atgaagattg tgaaaacaac catgcgcaac
     3121 ccagttcaat ttcggcgctg ccacgggatc gcgatctcgc ctaccgattt aaatgaacac
     3181 ggaaccacgg acaatgttcc catccacaac ttcaagaata ccccgtccgc ctggcgcggc
     3241 gggttggaag ggggggaaat ccaacatggc agtgaagaag gctaagaagg ccgctcctaa
     3301 gaaggcggcg aagaaggctg ctcctaagaa ggcggcgaag aaggctgctc ctaagaaggc
     3361 ggcgaagaag gttgcgaaga agaagtaatt tcttcccctt gcggccggcc gtgcaacgcg
     3421 gtcggcaaat gaacggcggt gattcctctc tggattcacc gtcgtttttc attcggtcga
     3481 tggccggtgc ggacgggttt cccggtgcca cttgccaagg cgggcaacgt ggagtaggtt
     3541 tccgcccact ggaaacagga gacgaccatg caagaaaaag tggaacagtt tattgaggaa
     3601 atccggccgc tgatccaagg ccacggcggc gatgtcgaat tcgtgtccat ggaaggcaac
     3661 accgtgcacc tgcggttgca cggcgcctgc cattcctgcc cgcacgccat gatgaccctc
     3721 aaaaacggca tcgaagctga aatgcgcgac cgcctaggcc ccgagctgac cgtggaacgc
     3781 gtgatggatg cctgacccgg cgcggcaagc agccgatcaa cccaacggcc gtcccgcggg
     3841 acggccgttt tgcgtggcac gatcgaagcg gaaatcaggc cggcggggcg ggatagtggc
     3901 cggcttgcgc gttgcggacg atttgtttgg tgtcctccgg aaaatcggag cgtagcagcc
     3961 acgtcaggaa attgggatcc ttggccacca cctcgcgcag gggttgcccc tgcactttgc
     4021 caaagttgaa cacaatttcg ccgcggctcc atttcagccg cccggtgcgg tccacccaac
     4081 tcgggtcgcg cgggtcgcag tattcatgca ggccgtccag atcggcgggc aattccgcgt
     4141 atttctgaaa ctggccaccc agcacgcgaa tggtggccaa cacatcgccg agcgcatcgt
     4201 gcgaatcggt gtggtcgtcg gcgcagtaat agcggagcgc cgcggcgagg tcgcgcggct
     4261 cgttgcggaa gaaaatccgc tgcgcgtcca ggatccgccg gccttccagc gggaacgggt
     4321 ggccggcgcg cgcgaattct tcggtcagaa tctggatgtc atagcccagg atgttgtatc
     4381 ccgcgaggtc cgcgcccgca aaatgatcca gcaggatttc ggccatgtcg gcaaacgtcg
     4441 gcgcgccttc cacgtcggca tcggtaatgc cgtggatggc cgtggcgtcc tcgggaatcg
     4501 gcacgccggg attcagcaga tagctgattt tctgggcggt gccagaaggt tcatagcgca
     4561 ccagcgcaat cgccacgatg cgatccgtgc gcttgttcaa gccggtggct tcaatgtcaa
     4621 aaaacaccag cgggcggtca agccgcaggc ggtcgggcag ggcgggaatg gcggtgtcca
     4681 tcatggacca caggatgacc gaacccacgc gcgagggaaa gcagaatcgc cccgccgctg
     4741 gaaaaatagt tgacgatttg tcttgcgggc cattccgggc ctgatatgtt gcgcggcata
     4801 ccttccgcat attcatgggt cgccggtttg cggggggcgg gttaatgcgc ggcccatggt
     4861 tacatggagt tgatagttac gtgaaaatat atgtcggcaa tctctcgttc aaggccacgg
     4921 acgatgatct gcgcgcggct ttcgagccgt acggcgaagt ggtctccgca cgggtgatca
     4981 aagacaagga aaccggccgc tcccgcggtt ttggtttcgt tgaaatgccc aatcaggaac
     5041 aaggcaacgc cgcgatctcc aaggtgaaca acaccgatat cgtggatcgc cctgtgcggg
     5101 tgaacgaggc gcaggaacgc ccccctcgcc gctaagttcg aataccaaac aagccccggc
     5161 tggttttccc cggccggggt tttttgttgg tccggcgcct caattcccgc gtgttgcggt
     5221 tatttgtaca cgtcccgccg gttcccaatc cgcaaaacca aaacctcttt gtccaacaaa
     5281 gtgaagatca cccggtaatc gcccatgcgc attttgcgca atccggcaaa ggggccgacc
     5341 aaggccggga agcggcccgg gcgggtggct agctcttttt cgatcttggc cagaagctgc
     5401 cgggcggcgg ccttgtccag ccgagccaaa tctttggcga cactcctttt atactggatg
     5461 ttataggcca agggacttcc tcagctcgtt gccggatact gccggatccc ggggattgcg
     5521 cagccggtcc aacgcgattt gcaggtccgc atattccgca atgtagtttt ccagcgcctt
     5581 ttggatgtgg aatgaacggg agcgttccgt ttcttcggca atttggttca gctcttcggc
     5641 caaaggcacc ggcacacgaa tggataaaat ggcgctcatg gttcacctcc tgtagtcatt
     5701 tgtaatacaa tgtacacaac gggagattcc gtggcaagca gggaatggcg actggccgcg
     5761 cggggagatg accgagcgtt tgccgggggg gaagctaacg cagggcgcgc aggagggggg
     5821 gccagcggtc gggcgggatt tcttcggcgc gggcggtggg ggcgatgccc acttgcgcca
     5881 gcgccggcag aggatcgacc accacgccgc gcagggaacg cgccagcgtt ttgcgccgct
     5941 gcgaaaaagc gtggcgcagc agctcgcgca gggcttgttc atttgccgcg ccacccagcg
     6001 gcgcggcccg ccgcgtcagc ttgacgatgg ctgactgcac ttccggcggc ggccagaagc
     6061 acgacggcga aatattcttc accaccgcaa tctcgtagtg gcgttgcagc aggatggcca
     6121 gcagcccgta gtctttaccg ccgggtcgcg ccacgagccg ctcggccact tcgcgttgga
     6181 tggtcaccac gatcagctcc ggcgggtggg gcagggcgcc cagctccacc agcagtcgcg
     6241 cggcgatagc atagggcagg ttggctacca gcttgtgccc tggggcggca aacaacgccg
     6301 gcaagtcggc ttccagaata tcgccttcgt gcaggaccag gcgcggttct tccgcgaagc
     6361 gcgtacgcaa atacgcggct agccgatggt ctttttccac ggcactcacc gcgctcgccc
     6421 tcatcagcaa ttcgtcggtt aacacgccca gtccggggcc aacttccacc acgcggtccg
     6481 tcggctggag ttcggccgcc gccagcagga tgcccagaat gttgcgatca atcaaaaagt
     6541 tttgccccag aatgcgcgac ggatgaaaat ccagttgcgc caacagggcg cgcacctctg
     6601 aagggcgcgt caggatgggc ggctcaatca tttttggtga ttccgttcac ggcgcaaacc
     6661 tgctaccagt tggccaaggt aggagctgcc atgagagatg ccgagcaaaa aatctggacg
     6721 cgggaagcct tggccggcga atgtacgcgg ctgcgcacgg ccgggcgcaa aattgtgttc
     6781 accaatggcg cgttcgattt gctccacgcc ggccacgcca cctacctgca gtttgcgcgc
     6841 cggcagggcg atgtgctgat tgtcgcgctc aataccgatg ccagcatccg ccgctacaag
     6901 ggcgaaaagc gccccatcgt ggatggcccc aaccgcgcgc gtctcatggc cgcgctggag
     6961 tgcgtggatt acgtggtgtt tttcgacgag gacgaaccca aggcgctgat cgccgagttg
     7021 cagcccgatg tgctggtgaa ggctgaggat tgggcgcact acgtcagcgg gcgcgacatc
     7081 gtggaagccc gcgggggcaa ggtcgtcctc gccccgatgg tcgccggcct ctccaccacg
     7141 aagctaatcg aaaagattct gcaagcctac ggcgcggggt agagcaggaa cgtcgggtag
     7201 ccctgaatgt tgaagggcgc cagcgcgtcg cgcgcgggcg gcttgttggg ttcatcggcc
     7261 aaaatctgga tggcggcata atccgccaac ttggccacga cgtccgcatc gcgcagggtg
     7321 gtggcatcca tcagcttgca caccttgcac cagtgcgcgc cgaaatcgag caggatgggc
     7381 ttgccggagg ccacggcctc gccgagcagc gcggcgaatt cttcactggt gccgctcttg
     7441 aaatcgaacg aacgcaatgc cgtcccgccg gtcgtgggcg cggcgccttc aggaacagcc
     7501 ggcacagcgc gcagcgcggt gtaggcattc caaccgtagt aagccgccag cagcagaatc
     7561 agcaccccga acgccttttt gactttctcc atccatgcgc ccggtttggg cagcacggcc
     7621 aatcccgccg cagccagcgg ccacggcagc gccatcccca cacccaacaa aaacggcaac
     7681 gccagcgcgg cggcaatccc ctgcccatag agcgaaccgg ccagcgccag cacagcaatc
     7741 aataccggcg ccacgcacgc ccccgccagc agcgcggaaa cgccgcccat caccagcacc
     7801 gcgggcaagc ggatgcgtcc cggcacgccg cccgttttgc ggaagcgcga aaaatcgagc
     7861 tgccacacat cgaacatcgc cagcgccagc gccacgaaca gcaccgcgat ggccaagttg
     7921 aaaaccggcg atgaattgat ggcgccgaac accgaacccg tcagcaccac cactaatccc
     7981 agcgcgccat aaaccagcgc catgccgagg ccatacacca gcccaaggcc gaaacgcatc
     8041 gcgcgcggcg tatccgcgcc gcccgcgcca atgatggcga ggttaatcgg aatcatcggc
     8101 aacacgcacg gcgtcaggtt gagcaaaatg ccgcccacca aaatcagcag cagtactggc
     8161 agcaagccgc ggcgcaaata aaactgcgcg gggtcatcca gaaacagccg ccacgcggaa
     8221 gccggagccg gcggtgcggc tgcggggtcc aggaacgcga gaaattccgg cgcatcggca
     8281 tagcccacca agcgacgtgg cgcgccgcgc tgcgcgagca agtcgggcca gtcggtcgtg
     8341 gatgccgcga tggccggcgc ggattcttcc gctgtggcgg cgccgagcga tagcgttacc
     8401 tcttcgggca aatagcacag ctcgcccttg cagccttgga aacccaccac aatgcggtcg
     8461 cccgccggcg gtttataaaa cgccgtgaag gaggtgccgt aggagggctc gatatcgtcg
     8521 ggattattcg tgtccggcgg cggcacaaac agcggcgtca acgccgtgcc cgccgtgtcc
     8581 tgcacggtaa aactctcaaa caggtgatgc ccttccggca cggtaaaatc cacgcgcacc
     8641 gcgggctgct gctcgtgggt ggtcaacgcg gcagacaccg caaatggcga ggcccacgcc
     8701 gccgtcccgc tgaacaatgc gacggccacc aaacttttcc acacaatgga aaaactttca
     8761 cggatttttc cacacaatgg aaaaactttg aggaattttt ccacagtgtg gaaaaaagtt
     8821 ttccacagtg tggaaaaatg ggcggggcgc gggatcatgg ttcatcctcc gagggggcag
     8881 gggcggcaag gagcgcagcg atatcgtcgc gcagggtgtc catggggaca aagccggtat
     8941 aggtgtgggc aaggttaccg gcgcgatcca aaagaaacag agtaggcacg gcgcggatgg
     9001 cgctgaggcc gccgaaagcg gtgaccaccg gcacatcggc caaccccagc gggaattcgg
     9061 gcgcgagttc ggcggcttcg gcggtgatgg cggccagggg gcggtcgtcg gccaccacgc
     9121 caatgacggt gaagccggcg gcggcgtaac cctgctgcag gcggttccac tcggcgaggg
     9181 tggcgcggca gccgggatcg tcggtcagca aaaagatgat gagctgcact tgcccggcgt
     9241 agtgcacggt ttcaatttcg ccgggaaatt ccgacgccag gtttttcaac gtgaaatcat
     9301 ggatgggcgg aatggggacg ggcgccggag gcacaggccg gcacgaacac gaggccagca
     9361 acaggcccag cgcgccagcc agcagcaggc gaaccaaagg atcgcgccca cgcggggcgg
     9421 gggcggcgtt gttcaacaaa acgggttgcg ttgtcttcat gcttccgatt cgccgccgac
     9481 ttggaaacgc ggcggcggca gcttcttggg actaatgata ttctgatttc ggaaattttc
     9541 catttggcgc agcaaattgc cgtcgccggt ggataatttt ttgaaggcgt tttcgtaggc
     9601 attgcgcgct ttggcgaggg cgtcgtctat ctgctgcatg tcttcggcga agttgacgta
     9661 tttttcgtaa atcttgcgga tggtatcgaa aatgctggtg atggcttggc gttggttttc
     9721 ctgtcgccag agttgcgcca cgaggcgcag ggtggccagc aacccgccgg gaccggtcag
     9781 gatgatgttg gctttggcgg cggtttcgta gagcgcaggc cgggcgtaaa gcgccaacgc
     9841 cagcgcgggc tcggagggca caaacatcaa cacaaagtcg ggcgagcggt cgggaaagag
     9901 gtcttggtaa cgccgtttgg caagattgtt gatgtgggta tcgaaggcga cggcgtgggc
     9961 cttgagcgta gcggtgcgcg cgggctcgtc ggtggcattg acgtgcgcga ggtagtcttt
    10021 cagtgaagtt ttggcgtcaa taattacgca gcggttgtct ggcagattga tgaggacatc
    10081 gggctgctgg cgctggccgc cctcgctggt ggcgctgact tgtgtttggt agtcgcggcc
    10141 tttttccaag ccggatgcgt caagcaggcg ttcgagggtc agttcgcccc agttgccgag
    10201 ggtcttgttg tcaccgcgga gcgcggtggc cagttggccg gtttgctggc tgacggcttg
    10261 gctttgtgtc gccaacagtt ccacttgctg gcgcagggcg ctgcgttgct ggctttcttc
    10321 ggaatggaga tgctccacct tttgcttgaa gtccttgatt ttttcatcaa gggggccgag
    10381 cagctcgctg agcgcggcgc gctgctgctg ggtaaattta gcggactgct cgccgaggat
    10441 ttgcagcgag agccgttgga attcatcggt gagtttcgtg cgcgccgcct ccagcatttc
    10501 cacctgggtt tgggcgcgga tggtttcggc gcgggcttga gccacttgcg caacggcacg
    10561 gaagtgagcc agcaaatagc ccagcgtcag gccgacagcg aggccaatca acaaatacag
    10621 cgtgaaggtc atggaggcga gttagccgca gcccgccatg gaaatcaacc gaaaaggggg
    10681 ggcggtgcgc gccgtgagtt ggtccgcggg gaaatcaggg cttgcggtca ggcaggcggc
    10741 agcgtagatt gcggcccaag tttgtccgga aaaagtgtag gacattgcga cggatggcgc
    10801 gcagcgatcg gggcggagcc aagcaggcgg ggttaaggga ttggttgcgg agtgcggccg
    10861 cggcaattgt cgttttcgga caagacaaca agagaaggat ggaagtctca tgggcttcat
    10921 gaatacgatc aaatcgctct tcggcgggtc caatggttcg gcccggcggg atgcgcgcac
    10981 ctatgtggtg gacgcggcgc gattggtgga tgagaaaacc gggcggcgac tcgccccgcg
    11041 cgagcaggtg caactgctca acgcgctggc ccgcgtggcc aagcaggaaa atctggtgtt
    11101 ccatgcggtg ttcgaaagcg aacgccccct gcgcgaagtg gaacacggcg gcgagttcaa
    11161 cggcgtgaag gtgtactttg cggccgatag cgagaagctg gtggagcagg ccctcaaact
    11221 gtgcaagagc gaaggctccg cattgatcag ctccggcgcc acgctcgaaa gccgtgctac
    11281 ggaagaaggc attgccgtgc tgtgcagcag tacgttccgc aaggcgtttt tgccctccgg
    11341 cctgcccagc aacgagcgca atggccgggg tgaccgtggc agccgtgagc ggcggcgtga
    11401 ccgcggccca cgccgtgagc ggaacgatca gcgcccgccg cgccccgaag ccgcgcctgg
    11461 cgcgtccgcc ggcgacaacg ccgcgcccga taccggcgcc aacccggacc ccgccccccc
    11521 gcaagaggac aacagcgcgg tgcgcaacct gattgatctg gtggaataac cctccccaga
    11581 ttccccaagg ccgtggcccc gccccggcct tttttgttgg acactttcgc cacgcgcgcc
    11641 agttccccgc tgtgatgcca cccaatggac attatgaaga ttgcgcgaag attgcacatc
    11701 cgctacaagc tgacgatagg tgccaaccca tgagtgttcc cgccatcgtc catctgatgt
    11761 attttccctg gggttgagaa ctaactccca cggggaaacc tgattgcttg ttcgactgat
    11821 acggaggaaa atttatgcaa ctactttcaa ggctgtacag gtctaaaccc aagctccaga
    11881 tttataaaat ccggctcgaa aatgatggta actccatccc gcaagaatat gttgaccacc
    11941 tcgccagatg taagccaagt ggcgccaact tatgcttgtg ctccaatacc caatagtatg
    12001 caaccttttc gctttgctca atatcgacag acttttcaag cctgtcgaca ctggaggtgg
    12061 cttccttgga tcggattgga tgcatttcgt attgagaaag ccgttaccag aaacaatgcc
    12121 catctgatca ccactcgcca ccatttctac aagatcggga tcggttcaac caacacagtc
    12181 aggcttgagt acaacaaata tcgccagatt gcgcttcact gtcaggaatt aacttcccac
    12241 ctgcctcaag tcagcttttc aactcaatgc cgccgccctg tcctacaaat gaaacgaatt
    12301 ccgcaaaccg acatgaccga catacaacat cctcaagcgg cgcgggtctt ggacatctta
    12361 aaacgatatt cacatcccgg cattcctgcc gactgggaag cattccctta cct
//
LOCUS       DAOPNP010000072        12234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6009_length_12234_cov_8.132934, whole genome shotgun sequence.
ACCESSION   DAOPNP010000072 DAOPNP010000000
VERSION     DAOPNP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6009_length_12234_cov_8.132934"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="PKI64_07525"
     CDS             <1..1657
                     /locus_tag="PKI64_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00894.1"
                     /translation="GQLRDKTYGIDYIVSLYNRINSAGRKVEQQEIAYATIVHLYPET
                     TACLKNFFKDVHPQKGVERDTLLRREKERNFGFKLYLRTLVQVCNYHFGRSQGTQMFS
                     FDILKSSDFSGQITQNREYAGMFFKWTHMILVFVHEVLCDSLFCDDLRMLPDIDCLIP
                     ALQLLIRYPRLIDTRYKQIIAYLILRTAIDPERTQESTLKLVRYIERTHDAGSCIKGL
                     PTQFGAWSEDKGNRFRNALASANTMNDRYVLLLYWLARRTGAKDFSYANLSSNKPLSH
                     RAGKEMVLSTEVVPEKQHIAPAADLSRDIYGGDSRITTSRHPINNIGNLTYISRELNH
                     FESGLGPQWIDPTKETDPAVLAGHFLQGEALDLYEKLRSRALSKADKDLFEQQYCKAR
                     RELIQAGFEAWMDELQKGLDVVDERIKPKKKLIVPDTADKIRWLDYPNRIEDALLMML
                     GGQMLKLMSEKAKKNKPGDIIFQLQDSGSKSKANIFMFRVPRRIEIEHRDGHGVLAKL
                     ESIMEQAGIEKQPNQARPWVLPCGPDEESLTAGILEVFYLNRL"
     gene            complement(1859..2878)
                     /locus_tag="PKI64_07530"
     CDS             complement(1859..2878)
                     /locus_tag="PKI64_07530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOE00895.1"
                     /translation="MKKYNVCMVGIGAVGTEIVRLLRKRNFPLGKLTVFATRERTEMI
                     DDIPVEVKVATGPESFKGFDFAFFAGTEGAKGASKTLGWGAAETGCTVIDNGDDYRMD
                     PRVPLCIPEINAATLKNHQNFIANPNCSTSTALMAIYALHNEAKIKRIVCCTYQAVSG
                     TGSAAIRELDAQVRAWAAGEPLPREVYPVQIAFNVIAQIGSESKDMPGYTSEEVKLGR
                     ETRKILGDDSIQVVSTCVRVPVFNGHSEALHVEFHNPITPERAREILAKTPGVALRDD
                     LAHSVFPTPLEATGSYDTLVGRIRKDPTVPHGLSLFVSGDNIWKGAAQNAIQIAEKMI
                     EMGLK"
     gene            complement(3026..4090)
                     /locus_tag="PKI64_07535"
     CDS             complement(3026..4090)
                     /locus_tag="PKI64_07535"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOE00896.1"
                     /translation="MKKTYSIGLIPGDGTGPEVTREAVKVLGAAAAKFGFGLDYTTYD
                     FGGDRYLKTGETLPDSALEEFRKHSALYLGAIGHPDVKPGILEVGILLKTRFALDQYI
                     NLRPVKLYRAEDCPLKDKEPKDVDFVVVRENTGGIYTGVGGAVQQGTPLEVATQVMIY
                     SRPVVDRCLRYAYEYARRRNRKKMLTLVHKCNVLTHCGDLWVRAHKEMGDKEFPDIKQ
                     DYNHVDACTMWFVKQPEWYDVVVTENLFGDIITDLAAIVQGGLGVAAGGNINPEGVSM
                     FECMGGSAPKYTGKNVINPVAAIAAGGMMLDTLGETAAAVAVDQAITAALNGGKIKSL
                     AAGRMGLGTSEVGDLIASLV"
     gene            complement(4186..9327)
                     /locus_tag="PKI64_07540"
     CDS             complement(4186..9327)
                     /locus_tag="PKI64_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOE00897.1"
                     /translation="MKFGKHIAIVGILATALAPVRGFAAEISGQVISRADGTPLHNIY
                     ASAYRQNGNSWQLMNGAYTDSAGNYTIKWLSAGTYRVQFTHWGGAYLQTHYTNALDLA
                     DATDVPVNSETSKVNGINVTMRTIPTFTGRVTGPDGETPLAGIQVTAYLRTGGGWQEK
                     SCAFTDTYGEYSILWLDPGTYRVYFADWSGPYLNEYYNRADDLNSAQDIVFPRDEDVA
                     NINAALRYKPTISGRVTGPDGVTPLAGIYAAAFQWTGTAWQEISSCTTDAAGNYTIKW
                     LGLGTYRVQFFEWSGKYLGECYPHADTLADAADIVMGTNTVYTGIDAALRPVRSISGR
                     VTDRQNQAALSGIAVTAYKSAGASWQSVSNRLTDADGFYVFSNLVAGTYRVQFADPAG
                     AHHGEYYCQAPDLAAANDIVLNATSAVRNVDAALRPLASISGRVKAAGNEAPVPGIRV
                     VALRQCENCWHEASSGYSDEAGTYALSGLEAGAYRVAFEDPAGHFVGEYYDCSDDISE
                     AQDVIVAQGAETTGVDAILEAASGVSGRITALASGEPLPNITVTLTLVGEWGPGFYQI
                     TSTDDNGEYEFQGVEAGTYRLEFRDNNLDYIGQFYDQQTDWFSATLIYVPESTLVTGL
                     NAALVEGAKLSGTVTDTQGAPAPGINVSCERWTGSYWQWMTGTLSDDDGQYTLRGLPA
                     GDYRVWFWDPNGQFISEYYDDQIDWNEATSVAVATATHVTGVDATLAKAGTISGTVTG
                     QAGAPLADIAVTVYRQQGGQWWWQENAMTDENGHYTIGRLPGGLYHVYFTDWQNDYLP
                     EYYNDATEYDDAEILMLPAEGALTGIDAELTKGATISGMLTGPDGQTPAGGVTVTAYM
                     YTNHYWYWVGSSSSAQNGMFTIKSLRAGTYRLQFNDYNETYLAQVYAQGNTLDDGTDI
                     PVATGEDVTGIDASLRLAARISGTVTAEDGVTPLANIAVIVGSRNGMGMNFQGNTDDN
                     GQYTIRGLPAGSYIVQFVDYQNAYLREFYDDAQTRDAATEFTLASGDWLTGIDATLGP
                     GSRLSGRVTAAATQSPVAGISVSALHWNGSWWDTWGSATTDDNGEYSLGGLMPGSYKV
                     QFQDWSHTYLPEFYNQAWTMEQATVVQVSADEDVAEIDAALSVAAQLSGRVTSATTQQ
                     PLAGISVTAVLWINSSWGASQHATTDSNGEYTITGLMPGTYRVFFTDYTQTHSTEYYD
                     NAPSIASATDIPVASQAHITGIDAALESASSIAGTVTALGGSPVLANISVQAFRWDGA
                     KWAWAGWAETDAAGNYTFNGLAAGEYRLQFHDQSGNYAGACYSNVPSLDAANSIALAA
                     GQQFTGINMHLGPAAWISGCVTGPDGSTPLRNIEVNVGRWTRHGWQLVNGTYTDQNGE
                     YSLGGLRAGEYRVQFFDHEGVYATEYFNDKRLLDEAEDIVLAVAAHLPGLNASLAYAG
                     SISGQVTAQDSGLPMPSLWVTAYTWTCEGWQEVAWTGTDENGAYTVGSLTPGTYRLGF
                     RDPSETYATKFYNNAPSAESAGNITLTDYDHLTGYNVALERGASIAGRVTGSNGETPL
                     AGIEVEAHYWTGNNWAPAARDFTAADGTYQIVNLLPGTYRVQFLDHSLTHAGQSYANA
                     ATLAEGTDITLAAGQQVNGIHAALQPLAKPEPPVMISIADCKSGNCRFTIMESVGRKY
                     VLQGNTALDKEWGDLGEPFYCAPGTNSIVRGSSKTFLYWRIRAVQ"
     gene            complement(9715..11517)
                     /locus_tag="PKI64_07545"
     CDS             complement(9715..11517)
                     /locus_tag="PKI64_07545"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOE00898.1"
                     /translation="MKTKMVVRMFLTLGLAWTARAATFSPAPTTNDLNNPHKGFMLWG
                     TDYANGAPDNHYGATIYHIYVPWREIETSDQVFEWGRFETNHLLPILNDHPDATFVLR
                     PVADYPDGENSGITLFYSGGELARDFPAFLTNAPFSIPAYPYTSCDGDGPGITPDWNH
                     PAMITQLVQFVEAFAARYDGDPRLTAVQVGLLGLWGEWHQSGCDDRAPSNAVKIAVRD
                     AYAASFTNTPIQTRYPGNPDAVGVEFGFHEDYFPSFTAPCIYGFPECSDDGPWNMDYC
                     FRHVTPGSADNWKSNPISGESPLNGQKKTWSNDVADVMTVLRDYHFSFLGPAGGHERK
                     GNQANLNPMKRLLGYNYHIVRADWPDAVAAGYPFAITLAMTNSGAAPCYHPFMLEVAL
                     CATNGTPAWRGTFAYNLRELLPGQPVEITESFTATSLTPGTYSLRIGILDPRTGEPGV
                     RIQSTGEDGNRRITLGAIQVNDPWTTDSDGDGLPDAWEHQYFGGFTNADPNADSDGDG
                     QLNWEHYISGTDPTNPASYFRLEYVGRDSGFAVRLDSVTNRVYAIQRAADLELADWIN
                     WHTGVAGNGAPLEFPETNPVSPGFFRAGVRLAPF"
     gene            complement(12062..12226)
                     /locus_tag="PKI64_07550"
     CDS             complement(12062..12226)
                     /locus_tag="PKI64_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOE00899.1"
                     /translation="MVEDRGGDIKGRHRLDLYYNTHNEARQWGRRRVDVTIFPPGTAP
                     IPQNARPPSL"
BASE COUNT         2498 a         3446 c         3647 g         2643 t
ORIGIN      
        1 aggccagctg cgggataaaa cgtatgggat cgactatatt gtttctctgt acaaccggat
       61 aaatagcgct ggtcgcaagg tggaacagca ggaaatcgca tatgcgacta ttgtacatct
      121 ctatccggag acaactgcct gcctgaaaaa ctttttcaaa gacgttcatc cccaaaaagg
      181 ggtcgagcga gatacgcttc tccgccgaga gaaagaacgc aatttcgggt tcaagctata
      241 tcttcggaca ctggtccagg tgtgcaacta tcactttgga cgttcccaag gcacacagat
      301 gttctcattc gatatcctta aaagtagtga tttttcaggg cagataactc aaaaccggga
      361 atatgccggt atgtttttta aatggaccca catgattctc gtgtttgtac acgaagtatt
      421 gtgtgattct ctattttgcg acgatctacg gatgctgcca gacatcgatt gtctcattcc
      481 cgcacttcag cttctcattc gttatccacg acttatcgat accagatata aacagattat
      541 cgcgtatctc atcctgcgga cagccattga tccggaacgg acccaggaat caaccctgaa
      601 gcttgtacga tacattgagc gcacccatga tgcgggttct tgtatcaagg ggctccctac
      661 tcagttcggc gcttggtctg aagacaaggg aaatcgtttt cggaacgcct tggcgtccgc
      721 caacactatg aatgataggt atgtcctgct gctatactgg ctggctcggc gcaccggggc
      781 caaagatttt agttatgcga atctgtcgtc aaacaaaccg ttatctcaca gagccggtaa
      841 ggaaatggtc ttatcaaccg aggttgttcc tgagaagcag cacatagcac ccgctgcaga
      901 ccttagccgt gatatctacg ggggcgattc gcgtataact acgagtcggc atccgattaa
      961 caatattggt aacctgacct atatttcccg agaattaaat catttcgaga gcggtcttgg
     1021 ccctcagtgg atcgatccaa cgaaggaaac tgacccggcg gttctggccg gtcattttct
     1081 gcaaggagag gccctggacc tgtatgaaaa gctacgtagt cgtgccttat ccaaggccga
     1141 caaagatttg ttcgagcagc agtattgcaa ggctcgacgt gagctgatcc aagctggctt
     1201 tgaagcatgg atggacgaac tccaaaaagg ccttgacgtg gttgatgaga gaatcaagcc
     1261 gaagaaaaag ttgattgtac cagatacagc agacaaaatc cgctggttag actacccaaa
     1321 tcgaatcgaa gatgcacttc ttatgatgtt gggggggcaa atgctaaaac tgatgtctga
     1381 aaaggcaaaa aagaacaaac ccggcgacat aatatttcaa ttgcaagatt caggatcaaa
     1441 atcgaaagcc aatatattta tgtttagggt tccccgcaga attgaaatcg agcatcgtga
     1501 cggccatggt gtgctggcaa agctggagag catcatggag caggcgggaa ttgagaaaca
     1561 gcctaatcag gctaggccat gggtattgcc gtgcgggccg gatgaggagt ctcttacggc
     1621 cggaatcctg gaagtttttt acctcaacag attatgactt gggattttcg accggcatga
     1681 gcgttctcaa ttgaaccaat cctcgtaacg gatggagagg cagttaaggg tagccttgta
     1741 gttgcgccag ttggggtggt gatgccgtat cccactcgtt gcttttgcgt tcggcgggga
     1801 agggggggat aaaaaaaggg ctctggcgac gagccagagc cctgtgggtg ggtgggggtt
     1861 acttgaggcc catttcgatc atcttttcag cgatctggat ggcgttttgg gcggcgccct
     1921 tccagatgtt gtcgccggag acgaacaacg acaggccgtg ggggacagtg gggtccttgc
     1981 ggatgcggcc cacgagggtg tcgtagctgc cggtggcttc tagaggtgtg gggaagaccg
     2041 agtgggcgag gtcgtcgcgc agggccacgc cgggggtctt ggcgaggatc tcgcgcgcac
     2101 gttccggggt gatgggattg tggaattcca cgtggagcgc ctcgctatga ccattgaaca
     2161 cggggacgcg cacgcaggtg gagaccacct ggatggaatc gtcgccgagg attttgcggg
     2221 tttcgcggcc gagtttcact tcttccgagg tgtagccggg catgtccttg gattccgagc
     2281 cgatctgggc gatgacgttg aaggcgattt gcacgggata gacctcgcgg gggaggggct
     2341 cgccggcggc ccaggcgcgg acttgggcat ccaactcacg gatagccgcg gagccggtgc
     2401 cggagaccgc ctgataggtg cagcacacga tgcgcttgat cttggcctcg ttatgcaggg
     2461 cataaatggc catcagcgcc gtggaggtgg agcaattcgg gttggcgatg aagttctggt
     2521 ggttcttcag cgtggcggcg ttgatttccg ggatgcacag aggcacgcga gggtccatgc
     2581 ggtagtcatc gccattgtca atgacagtgc agccggtttc cgccgcaccc cagccgagag
     2641 tcttggaagc gcccttggcg ccttcggtgc ccgcgaaaaa ggcaaaatcg aagcccttga
     2701 atgattccgg gcccgtggcg actttgacct cgacggggat atcgtcaatc atttcggtgc
     2761 gttcgcgggt ggcgaacact gtcagtttgc cgaggggaaa attacgcttg cgtagcagcc
     2821 gcacgatttc cgtgcccacc gcgccaatac cgaccatgca cacattgtat tttttcattt
     2881 ttctctcctg attgcttgcc gtacgaattg gtttttaccg ttaccgccgg cggtcatcat
     2941 tagccttgag atacgccatg acgagcgctt tgtaaaatgg atttccgctc cggcggtgtt
     3001 gctttgccgt tcgctggggt gggggctaga cgagcgaggc gatcagatcg cccacctcgg
     3061 aggtccccaa gcccatgcgg ccggccgcga gcgacttgat cttgccgccg ttgagcgccg
     3121 ccgtaatggc ttgatccacc gccaccgccg ccgcagtttc accgagggtg tcgagcatca
     3181 tcccgccggc cgcaatagcc gcgacggggt tgatgacgtt tttgccggtg tacttgggcg
     3241 cgctgccgcc catgcactcg aacatggaaa cgccttccgg gttgatgttg ccgccggcgg
     3301 ccacgccgag cccaccctgc acaatcgcgg cgaggtcggt gatgatgtcg ccgaacaggt
     3361 tttcggtgac gaccacatcg taccattctg gctgcttcac aaaccacatc gtgcaggcat
     3421 ccacgtgatt atagtcctgc ttgatgtcgg gaaattcctt gtcgcccatt tccttgtggg
     3481 cgcgcaccca gaggtcgccg cagtgggtca ggacgttgca cttgtggacg agagtgagca
     3541 ttttcttgcg gttgcgccgc cgggcatatt cataggcgta gcgcagacaa cggtccacca
     3601 cggggcggct atagatcatg acctgcgtgg cgacttccaa cggggtgcct tgctgcaccg
     3661 cgccgcccac gccggtataa atgccgccgg tgttttcgcg caccacgaca aaatccacat
     3721 ccttgggttc cttgtccttc agcgggcagt cttcggcgcg gtagagtttg accgggcgca
     3781 ggttgatgta ctgatccagc gcgaaacggg ttttgagcag gatgcccact tcgaggatgc
     3841 cgggtttgac gtcgggatgg ccgatggcgc ccaaatacaa cgcgctatgc ttgcggaatt
     3901 cttccagcgc ggaatccggc agcgtttcgc cggtcttgag atagcggtca cccccaaaat
     3961 cataggttgt gtaatcaagg ccaaagccga atttggccgc ggccgcaccg agcaccttca
     4021 cggcctcgcg ggtgacttcg gggccggtcc cgtcgccggg aatcaatccg atggaataag
     4081 tctttttcat aaggcggggc atttttagtt gatttggctg ctaaagcaag ccctgaaaca
     4141 ggctcgcggg caattggggc ggcccgcagc gggcgggacg gtggattatt gcaccgcgcg
     4201 gatgcgccaa tagaggaaag tcttggacga tccgcgcaca atgctgttgg tgcccggtgc
     4261 gcaatagaac ggttcgccga ggtcgcccca ctccttgtcc aatgcggtat tgccctgcag
     4321 gacatatttg cgaccgacgc tttccataat ggtgaaccgg cagttgccgc tcttgcaatc
     4381 cgcaatgctg atcatgaccg gcggctccgg cttggccaac ggttgtaacg ccgcatggat
     4441 gccgttgacc tgttgccctg cagcaagcgt gatatcggtc ccttccgcca aagtggccgc
     4501 attggcatag ctttgcccag cgtgggtcag gctgtggtcg aggaactgca cacggtaggt
     4561 gcccggcagc aggttgacaa tctgataggt gccatcggcg gcggtgaaat cgcgtgcagc
     4621 cggcgcccag ttgttgccgg tccagtagtg tgcttccacc tcaatacccg ccaacggagt
     4681 ttcgccgttg gagcccgtga cccgaccggc gatgctcgcc ccgcgttcca gcgccacgtt
     4741 atagccagtg aggtgatcat agtcggtcag cgtgatattg ccggcggatt ccgcgctggg
     4801 tgcattgttg tagaacttgg tggcatacgt ttcgctgggg tcacggaaac cgagacgata
     4861 cgtgccgggt gtcaggctgc cgacggtata agcaccgttt tcgtcggtgc cggtccaagc
     4921 cacttcctgc cagccctcgc aggtccaggt gtaggcggtg acccacagag agggcatggg
     4981 caggccggaa tcttgggcgg tcacttggcc ggaaatgctg ccggcatagg ccagcgaggc
     5041 attgaggccc ggcaggtggg ctgcgacagc caagacaata tcttccgctt cgtccagcag
     5101 gcgcttatcg ttgaaatatt ccgtggcata aacgccctcg tggtcgaaga attgcacgcg
     5161 gtattcaccg gcgcgcagcc cgccgaggga atactcccca ttttgatcag tgtaggtgcc
     5221 gttcaccaac tgccagccgt ggcgcgtcca gcggccgacg ttcacctcga tgttgcgcag
     5281 cggcgtgctg ccgtccggcc ccgtcacgca cccggaaatc caggccgccg gcccgagatg
     5341 catattgatg ccagtgaact gctgcccggc ggccagcgcg atgctgttgg ccgcgtcgag
     5401 gctaggcacg ttggaatagc aagccccggc gtagttgccg ctctggtcgt ggaattgcaa
     5461 gcggtattcc cccgcggcca gcccgttgaa agtgtagttt ccggcggcat cggtttcagc
     5521 ccagccggcc caagcccact tggcgccatc ccaccggaat gcctgcacgg aaatattcgc
     5581 gaggaccggg gagccgccca aggcggtgac ggtgcccgcg atggaggagg cgctctccag
     5641 cgcggcatca atgccggtga tgtgcgcttg cgacgccacc ggaatgtcag tggcgctcgc
     5701 gatgctcggt gcgttgtcgt aatactcggt ggaatgggtt tgagtgtagt ccgtgaagaa
     5761 cacgcggtag gtgcccggca tcaatccggt aatcgtgtat tcgccgttgc tgtccgtggt
     5821 ggcgtgctgc gaggcacccc acgaggaatt gatccacagc acggcggtga cggaaatacc
     5881 cgccagcggc tgctgggtgg tggcagaagt cacccggccg gacagttgcg cggcgacgga
     5941 tagcgcggca tcaatttctg ccacgtcttc atccgccgac acctgaacca cggtggcttg
     6001 ctccattgtc caggcctgat tgtaaaactc ggggagataa gtgtggctcc agtcctggaa
     6061 ctggactttg taggaaccgg gcatcaagcc gcccagcgaa tactcaccgt tgtcatcagt
     6121 ggtggccgag ccccacgtgt cccaccacga cccattccag tgcagcgcag agacggagat
     6181 tccagccacc ggcgattgcg tggccgcagc cgtgacccgg ccagacaacc ggctgcccgg
     6241 ccccaaggtg gcgtcaatgc ccgtgagcca gtcgcccgat gccagagtga actcggtggc
     6301 cgcatcccgc gtttgggcgt catcgtagaa ctcgcgcaga tacgcattct ggtaatccac
     6361 aaattggaca atataagagc cggccggcag cccgcgaatg gtgtattgcc cattgtcgtc
     6421 ggtattgccc tggaaattca tgcccatgcc gttccggctg ccgacaatga cggcgatatt
     6481 cgccagcggt gttacgccat cttccgcggt gaccgtgccg gaaatcctgg cggccagccg
     6541 cagcgaggcg tcaatgcccg taacatcctc gccggtggcc actggaatgt cggtgccatc
     6601 gtccagcgtg ttgccctgcg catacacctg cgccaaatag gtttcgttgt aatcgttaaa
     6661 ttgcaggcgg taggtgccgg cgcgcaggct cttgatggtg aacatgccgt tctgcgcgga
     6721 ggagctggag ccgacccagt accaatagtg gttggtgtac atataggcgg tcaccgtgac
     6781 tccgcccgcc ggtgtctggc cgtccgggcc ggtcaacatg cccgaaatgg tggcgccctt
     6841 ggttagctcg gcatcaatgc ccgtcagtgc gccctccgcc ggcagcatca aaatctcggc
     6901 atcatcatat tcggtggcat cgttgtaata ttcgggcaga taatcgtttt gccagtcggt
     6961 gaagtagaca tgatacaagc cacccggcag ccggccgatg gtgtagtgcc cgttttcgtc
     7021 ggtcatggcg ttttcctgcc accaccattg gccaccctgt tggcgataaa ccgtcaccgc
     7081 gatgtcggcc agcggcgcgc cggcttgccc ggtcaccgtg ccagaaatgg tgccggcctt
     7141 ggccagcgtc gcgtcaacgc ccgtcacgtg cgtcgccgtg gcaacggcca ccgaagtggc
     7201 ttcgttccag tctatctggt cgtcgtaata ctcgctaatg aactggccgt tgggatccca
     7261 aaaccacact ctgtaatcgc ctgccggcag gccgcggagc gtgtattggc catcatcgtc
     7321 cgacagcgtg ccggtcatcc attgccaata ggagcctgtc cagcgctcac aactgacatt
     7381 gatgcccggc gccggtgcgc cctgcgtatc cgtcactgtg ccggacaact tggcgccttc
     7441 gactagcgcc gcgttgaggc cggtgaccag tgtactctcc ggcacataaa tcagcgtggc
     7501 agagaaccaa tccgtttgct ggtcataaaa ttgcccgatg taatccagat tgttgtcgcg
     7561 gaattcgagg cggtacgttc cggcttccac cccctggaac tcatattcgc cgttatcgtc
     7621 ggtgcttgtg atctggtaga agccggggcc ccattcgcca accagcgtga gcgtgacggt
     7681 gatattcggc aacggctcgc cacttgccag tgcggtgatc cgcccggaca cgccggacgc
     7741 tgcttccagg atggcatcca cgcccgtggt ttctgcgccc tgtgccacaa tcacatcctg
     7801 cgcttcggag atgtcgtccg agcagtcgta atactcgccg acaaagtggc cggcggggtc
     7861 ctcgaacgcc acgcggtacg cacccgcttc caggccgctc agcgcgtaag tgcctgcttc
     7921 atccgagtag ccggaactcg cctcatgcca gcagttctca cattgccgga gcgccaccac
     7981 gcgaatcccc ggcacgggcg cctcgttacc cgcggccttg acccggcccg aaatggaagc
     8041 cagcggccgg agcgcggcgt ccacatttcg cacggccgag gtggcattca gcacaatgtc
     8101 attggccgcg gcgagatccg gcgcctggca ataatattcg ccgtggtgtg cgccggcggg
     8161 atcggcaaac tgcacgcgat atgtgcccgc caccagattg gaaaaaacat agaaaccgtc
     8221 ggcatccgtc agccggttgc tcacgctttg ccacgacgcg cccgcggact tgtacgccgt
     8281 aacggcaata ccagacaagg cggcctgatt ctgccggtca gtcacccgtc cggagatgga
     8341 acgcactggc cgaagcgccg catcaattcc cgtataaacc gtgttcgtgc ccatgacgat
     8401 gtccgctgca tcagcgagtg tatcggcatg gggatagcat tcgccaaggt atttgccgct
     8461 ccactcgaag aactgcacgc ggtacgtccc cagacccaac cacttgatcg tataattacc
     8521 ggcggcatcg gtggtgcaac tggatatttc ctgccacgcg gtgcctgtcc attggaaggc
     8581 cgccgcataa atccccgcca gcggggtgac gccatccggc ccggtcaccc ggccggaaat
     8641 ggtcggcttg tagcgcaggg cggcgttgat attggccaca tcctcgtcgc gggggaacac
     8701 aatatcctgt gcggaattca aatcatccgc ccgattgtaa tattcgttca ggtaggggcc
     8761 gctccagtca gcgaagtaca cgcgataggt tcccgggtcc aaccaaagaa tagagtattc
     8821 cccataggta tcggtgaagg cgcagctttt ttcctgccag ccacctcccg tgcgcaggta
     8881 agcggtcact tgaattccgg cgagcggggt ttcgccatcg ggcccggtca cacgcccagt
     8941 gaacgtggga atcgtgcgca tggtcacatt gatgccgttg acctttgagg tttcagaatt
     9001 gacgggcaca tcggtggcat cggccagatc cagtgcgttg gtgtaatgcg tttgcaaata
     9061 cgcgccgccc cagtgggtga actgtacgcg gtaggtgccg gcgctcagcc acttgatcgt
     9121 ataattaccg gcgctgtcgg tataggcgcc attcatgagt tgccatgaat tgccattctg
     9181 gcgataggcc gaggcataga tattgtgcag aggcgtacca tcagcccggc tgatcacttg
     9241 gccggaaatt tcggcagcaa acccccgcac cggcgcgagc gccgttgcga gaatgccaac
     9301 tatggctata tgtttgccaa acttcattcc aacccctgtt tcttctgcgt tcagagccgt
     9361 gcccacccgg gcgcgctttt ctgcagatga atatagatct actaatacac aagaatcgga
     9421 aatctatgcg gtagaagcaa gaaatatagt gttaaaaatg tgttaagaaa acgacaggat
     9481 cactgcgtta acacagatta gaaacaactg tgagttgcaa ataaataaat aaccaaaaaa
     9541 ccaaatgtta agattgtgtt aagaaacggt atacctgata ttattagtgc gcgcatggct
     9601 gctcgtacca gcgttcagaa aactgaaaat tccgccgcaa cacgttaggg cataaaccga
     9661 ccggatagtt cgacaggtga ggccttcggc cgtcgcgccg tatacgcgtt tgcgttaaaa
     9721 gggcgccagc cgaacgccgg cgcgaaagaa gccgggtgaa acgggatttg tttccggaaa
     9781 ttccagcggc gcgccattgc cggcgacgcc ggtatgccag ttgatccaat cggcaagttc
     9841 gaggtcagcc gcccgctgaa tggcatagac gcgattcgtg actgaatcca agcggacggc
     9901 aaaaccggaa tcgcggccga cgtactcgag gcgaaaatat gacgcaggat tggtcgggtc
     9961 cgttccgctg atgtagtgct cccagttcaa ctgcccgtcg ccatcggagt cggcattcgg
    10021 gtcggcattc gtaaacccgc cgaagtattg atgctcccac gcatcgggta gcccgtcgcc
    10081 atcggagtcc gttgtccacg ggtcgttgac ctgaatggcg ccaagggtaa tccggcgatt
    10141 tccatcttcc ccggttgact gaatccgcac ccccggttcg cccgtgcgcg ggtcgagaat
    10201 gccgattcgc agcgagtacg tccccggcgt gaggctggtg gcggtgaacg attcggttat
    10261 ttcgaccggt tggccgggca gcagttcacg cagattgtat gcgaatgtcc cgcgccacgc
    10321 tggcgtgcca ttcgtggcgc agagcgcgac ttccagcatg aacggatgat aacacggcgc
    10381 tgcgccgctg ttcgtcatgg ccagcgtgat cgcgaacgga tagccggcgg caaccgcgtc
    10441 gggccagtcc gcgcgcacga tatgatagtt atagccgagc aggcgtttca tcggattcag
    10501 attcgcctga ttccccttgc gctcgtgtcc accggccggg ccgagaaacg aaaaatggta
    10561 gtcgcgcagc accgtcatga cgtcggcgac atcatttgac caggttttct tctggccgtt
    10621 tagcgggctc tctccggaga ttggattcga tttccagttg tcggctgagc ccggcgtgac
    10681 gtgccggaaa caataatcca tattccacgg cccgtcgtcg ctgcactcgg ggaacccgta
    10741 gatacaaggc gcagtaaacg atggaaaata gtcctcgtga aaaccgaact caacgccgac
    10801 cgcgtccggg ttgccggggt accgcgtttg gatgggtgtg ttcgtgaatg aggccgcgta
    10861 cgcatcacgc acggcaatct tcacggcgtt ggatggcgcc cggtcatcgc agcccgattg
    10921 atgccactcg ccccagagac cgagcaggcc cacctgcacc gcagtgagac gcgggtcccc
    10981 gtcataacgc gcggcgaaag cctccacgaa ttggaccaac tgggtgatca tcgctggatg
    11041 gttccaatcc ggcgtgatgc cggggccatc gccatcgcaa ctcgtgtagg gataggccgg
    11101 aatcgagaac ggcgcatttg tcaaaaacgc ggggaaatcg cgggctaatt cgccgccaga
    11161 ataaaacagc gtgattccac tattctcgcc gtcgggatag tcggcgacgg ggcgcagcac
    11221 aaatgtcgcg tccggatgat cgttcagaat cggcaaaaga tgattggtct cgaatcggcc
    11281 ccactcgaac acttgatcgc ttgtctcgat ctcgcgccac gggacataga tgtggtagat
    11341 cgtcgcgcca taatggttgt ccggtgcgcc gttggcgtag tccgtgcccc aaagcatgaa
    11401 gcctttgtgc ggattattca ggtcattggt cgtgggtgcg gggctgaatg tcgcggcgcg
    11461 cgcagtccac gcgagcccga gggtcaggaa catgcgcaca accatttttg tcttcaattg
    11521 actctcgttc atccgcatct tatggctcgc ctatttgtac agcatactcg gcaaccgctt
    11581 ccacctccac gcaatatctc cccattctac ccgcaatcaa gaaagattct gccgcttggc
    11641 ttgttgggca tgaacgggcg cggaccttgg aatgggagac atcgctgttt tcagtgtatg
    11701 gagcgtctcg ctggggatgt ggaagcagcc cttatcgggc cctcttcgtt ttttcatttg
    11761 aaccatgctg tccacacttt ataaactctg gacagatgtt ataatatttc tatctcaatt
    11821 atacacaatg taatacatct aatttgcggt ttatttttaa tcatccattt tcagggagtt
    11881 gtccacactt tataaactct ggacagattt tgggtgggtg attttatgcg cttttctccg
    11941 tgcggttcgt tcccgcatgg aaggaaccaa tcccacgatc ctcgctgaaa tcggcgaatg
    12001 ttctgaaatg ctcgcaggcc tttcagccgt tcctggcggg tcaatgattc ccgctcgggc
    12061 tctacaagga gggggggcgg gcgttttggg ggatgggggc ggtgccgggg gggaagatgg
    12121 tgacatccac gcggcggcgg ccccattgcc gggcctcgtt gtgagtgttg tagtacaggt
    12181 cgaggcggtg gcggcccttg atgtcgccgc cgcggtcttc gaccacgccg tagc
//
LOCUS       DAOPNP010000073        12143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6077_length_12143_cov_5.074537, whole genome shotgun sequence.
ACCESSION   DAOPNP010000073 DAOPNP010000000
VERSION     DAOPNP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6077_length_12143_cov_5.074537"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>12143
                     /locus_tag="PKI64_07555"
     CDS             <1..>12143
                     /locus_tag="PKI64_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOE00900.1"
                     /translation="SLERSPANNDTNRRNDWRDQPTPNPGSGNLPQGLSSGAEITIVY
                     AVEINCAAVSGQLFARAGFEQPIGTATNATGSAFLTVNIPDLVVTKTPIMQEAGEGDE
                     VVWTVRIANEGFGTAQNVVASDRLGHGLAFTGFDPTPANATVSNAVWDSTVIPALASL
                     APGAYVDIVVTAQVVACKGLFNQADAKWGCQNLQVLANEICEDTSLENETATAGIRFL
                     DRFPSISYAMDPSPILVDYCAGTEVTLYITNASGAEVGTAYNMTGTPVMPPGWSLTGD
                     NVISNVLHFDPLPAGTATSVTFRVNAGGCPISTNEFYLYFRPYYEDACGTPFTGPIGV
                     SLARVINPPSASVTKIMPTSVSGDDGSIPVTVRLSYTNFTGSETITVTDVFPAHTNLT
                     APQNITGGGTLTSTSVVWTVSGLSGSGTWSANFDMGILEHCGGPRAIVYNTIYATTYT
                     DCKGCERTVTGSGERYPVQVRAGIGCETNACSFSSAKFISTTEAEVCEDVIITHVFSS
                     FTGSLTDWTGVTFFADLAENGWTDTNAVRVWIDSTDVTSYVAISTNASGMTIGLSGLN
                     GSPYPDMASVTSNLRVIYQVASASAGRQYDYSYLSTPQCGTEEDIVSWLNGAGRMAIT
                     LKPVMIAGACATTDGEIDLAILPLDGDTLYPVRDVVVTLNLDYDGNNFSAFHYEPGST
                     VFHNIVDPAGNPITASDPTISPSGYQWTWHLGDVGTNNYANMSITYKIHMGCTNDTSA
                     QHRAEVRYNDNCSLGQDPPARVAESGINGPPLFGTPVLFVNLQPEMQFLVDTQIVNKI
                     LVMNTCAIEANNLRVEIALPPNVSFAGAQISPSTVTATNVVWDFHTINQAFGPLQDMD
                     GDGSIDDLPPRQVFEFWVTNNVDYCAPLTELNLRASYGCYGDDCQVTPWDTAGYESIS
                     GSLVTRATFPVQNELCGTNPVVYSVRNSGLTVDYDVITRQTLPAGISYVGGSSQVSIN
                     GGALAPISDPSGSGLPGDPLVWDSSLIPLLAAMPPTNEVAIHYNVALGCEAVSGDNRF
                     IAEGVYTDLCDNILTNREIVSVLAPKEPLLVMTKTASMPVADADEEVVYTITISHDPN
                     SAAPVPYLKLTDVLPSAMDYVGASVLPDSVTGAGPGGQTLEWSATNLLAQIGDGLPPF
                     ALNEGPITILVTAKVVACEASVINQATLDYGCSAADACIQITANASIVTSPRLQPPGV
                     DGELVLDTCGGTKTVLVTNSGASTAFLFNYTEWAPAGYVFTGASVTGEFNASSAELIV
                     SYSGTPHGSVAHIDFSTKASSGATDAKDDAGDGIDNLDLGKNSGFVVTWTLRSTGENL
                     DCLADPTDLDFEDPEQGVPSLLGTSNLVTYTDLCHNPGEVTGSTSTYPDIPDLDIDLQ
                     PNSLIVTNGEVVNFKVTVINDSETVNADGIYVRVLLGEGWTNVTYVSSNIVSSGANAM
                     TMDQVGDTNLLFNFPGVILNPINDIIEINFTAQATTNGGALTALAEVVGDCGNSAITP
                     SCTFTNTLGGNPLANTMQGSTLGPVNGQYYSFDQDSFAAAGLELTKTVRYDEEPASAA
                     GTERDARVGEDLIYRIEAFYFGGTFTNVTISDSLPLGLHFGTPTVAATSGGITGGSWG
                     AGTGVFTLAPTAFSVPSSITLDIPVVVSNMMFVQDGVVITNVATTHFEVDGVTNSAPE
                     ASTEVEVLEPNLKITKVADKTSNIQAGDIITFTNIIEHTDLSRTSAYSVVFTDVLPAG
                     LQFNTWVTPASGQVLGQSITVDSTDLPALAEFKTNAPPIAIVFTVLVQDQIIGSTATN
                     ISVATYESLDSGSKNGNERDGSDGPGGLNDYYTDDPEVLNFEDLRGINKTFVSSSQQN
                     THDYATSNDWTIGERFIYQIRVDMPQGVVTNLVLTDTVPVGIDFVGTNPDAALAYPGK
                     GYEFEIPAGGPQFVEANVVVTDPDPSPASSVDTNGSGRAITFTFPAITNAADGNATND
                     YFLLRMEFVALDMPYNAGLRPNPRMGSNVVSVTDGTTTLGATGPVHRIVEHDIRVSKS
                     RTPLEGDAGDPITFTIVASNHVNALANAYKVVVRDYLTNSVYDVTSFTSLSTPPGWTL
                     NWQNHGTYGLYELSANEGVGLAPGTGVSAQFSINLAQTVRPNQVYQNTVTIPQSSTIS
                     NDVPTGIKDRQDGATNMVPHGIPGMSVSKILEATSETNNPPDSTGSNVQIGEVVTYRI
                     GVTMPESTITNLSVVDHVSNNGLAYIYGSARLDTGAWGPNAAFVGNTGNFTENPSGAL
                     GDLAPLGQAITFGFTNVVVTGDNNTTNNTFYILADYLVRSNAVNNGLGPNPTVHTNHA
                     TLTYQGNPGNTVTSAVVTTTVIEPKLEITKSLSPTNHVDAGDIISVTLVVTNSGTGTA
                     YDVVIEDELALPYFDISTLANVIVPTGFVHEVDGDILRFKSYEFAPTGTNTIEAGETL
                     TFTFDVHTGAGLPPNSVVTNTATVKGDSIKDNGPKNEQRDTNDEDEDQFTSDDFHPTK
                     ALIATSETGPVDSTGNNLQIGEVATYEIKVNLPEGVIPDLTITDVMPAGMAYVVGSAT
                     VDASEVSGAMGAFTVTPNVGVLGASGENVVMTWQGNSVITASTNHSHTANVLRVTMQA
                     VMMDLPANSGLPPQTVLTNKATVTFTGNPNPPAEIDGPPVTAVEPDIAIFKTVSPTNG
                     VDAGDEITITLVATNSGTATAYDLVISDVLSEDIFNLASVTNVSMPAGFVFATTPGAG
                     TLTVTYSSDPASSQPTNSLEVGESLEFVFTVHASQAVEPNSGYTNTVSLRADTIDGTP
                     PTPEEREYTEDDEDDFDTPKMSIAKVLTGTSENGPADSTGSDVQIGETATYRLTVTLP
                     EATITNLTVVDLVPVGMEYVAGSVAVDTNGFGGTLPAGSPTVTTLGGSGDDITIVFEG
                     NTVVTDNNDPSDNSFTITLTLLTLNVGSNTGKVAGAQTVLNNRANVSYDGKDPEDETP
                     DVPVVVIEPVLAIEKTMALIGLDVVEIKLVVTNSGLATAFDVEIQDVLTTNWWDTDTI
                     TNVTIPDGFTFSFAGNPGDATVTIASDPASGQPTNSIEVGEALEFVFRATLIPDAPSP
                     VTNRATVTEYSSKDGKDDDERKYPDEDDEKELPLPKYTLTKTLTSPLGRPAVVGETVT
                     FTIEVANTGDVGLGLVPLDDTYDATYLSFQSASPAETSTGPGSIHWDDIGPIGVGSSK
                     VVTVDFTAIESTLPSDTTNWVTTSPFTTNDIPLKPKTNEAPVEVRYAGFTLEKTRTSP
                     AGRAAQIGEEVVFTITVVNTGEVNLVTVPLEDTYETAFLSYIESEPASDDNNNDGVIN
                     WANIGPLAMGETAVVTSRFTAVATTTNQSRTNIVVASPTTTNTPPLPPQTNPAPYEIV
                     NADYTLIKEVVSPIGRAAEVGETVVFRLTVENTGEAPLVTVPVEDTYETAYLEYVSSV
                     PASDDNVNDGVINWADVGPIAVGDSVSIDVTFTAVASTGGEQKTNTVTTAPTTPPDYP
                     DVPPKTNEVPYEVQIVGYTLVKEVTSPLGRPANIGEEVVFTITVANTGEVALVTVPVE
                     DTYETAYLSYVSSLPASDDNINDGVINWANIGPIPVGESRSIVATFTAVASTTNSSRT
                     NIVVTAPETPPESPPVPPKTNEVPYEVLSSDYILTKEIVSPTNRAAEVGETVVFRLTV
                     ENTGEVELVTVPVEDTYETAYLEYVSSVPASDDNVNDGVINWTDIGPIPVGESRSILA
                     TFTAVASTEGDSKTNRVVTAPTTPPNTPPVPPKTNEVPYEVQIVGYTLVKEVTSPVGR
                     AANIGEDIVFTITVANTGEVALVTVPVEDTYETAYLEYVSSVPASDDNINDGVINWAN
                     IGPIPVGGSASIVATFTAVASSTNLARTNIVVTAPETPTNSPPVPPKTNDVPYQIVSA
                     SYKLLKERIRPADRPAIIGEEVVFRITVENDGEADLVEVPVEDRYETTYLSYVGSEPA
                     SDDNIDDGVINWTNIGPLPAGATTSIVATFTAVLNTTEVHTNWVETAPETPPGYPPVP
                     PQTNPAPYEVDIPARLGDRVWEDLNGDGIQDAGEPGIPGVKVTLFGTNGSVVATTVTD
                     V"
BASE COUNT         2566 a         3526 c         3825 g         2226 t
ORIGIN      
        1 attccttgga acgcagtccg gccaataacg ataccaaccg gcgcaatgac tggcgagatc
       61 agcccacgcc caatcctgga tcggggaatc tgccgcaggg attgtccagt ggcgcggaga
      121 ttaccattgt ctatgcggtg gaaatcaatt gcgcggcagt atcaggccag ttgtttgcgc
      181 gggcgggctt tgagcaaccc atcggcaccg ccaccaatgc cacggggtcg gcatttttga
      241 cggtgaacat cccggacctg gtggtcacca agaccccgat catgcaggag gcgggggagg
      301 gggatgaagt ggtgtggacg gtgcggattg ccaacgaagg ctttggcacg gcacaaaatg
      361 ttgttgcgtc tgaccggttg ggccacgggc tcgctttcac cggctttgac cccacaccag
      421 ccaatgcaac tgtctccaat gcggtgtggg actccacggt gatcccggca ctggctagct
      481 tggcgccggg cgcttatgtg gacattgtgg tgacggcgca agtggtggca tgcaaggggc
      541 tgttcaacca ggccgatgcc aagtggggtt gccagaacct gcaagtgttg gccaatgaga
      601 tttgcgaaga cacctcgttg gagaacgaga cggccacggc gggcattcgt tttctcgacc
      661 gcttcccgag catttcctac gcaatggatc cctcgcccat tttggtggac tattgcgcgg
      721 gcaccgaggt gacgctgtat atcacgaatg cctccggcgc cgaggtgggc acggcgtaca
      781 acatgacggg cacgccggtc atgccgccgg gctggtcttt gacaggcgac aacgtgatta
      841 gcaacgttct ccactttgac ccgctgcctg ccggcacggc gacatcggtg acgttccggg
      901 tcaacgcggg cggctgcccg atttccacca acgagtttta cctttacttc cggccctatt
      961 atgaggatgc ctgcggtacg ccgttcacgg gcccgatcgg tgtgagtttg gcgcgggtga
     1021 tcaacccacc ctcggcatcg gtcaccaaga tcatgccaac gagtgtttcc ggcgacgacg
     1081 gcagcattcc ggtgacggtt cgcctgtcgt acaccaattt cacgggaagc gaaaccatta
     1141 cggtgacgga cgtattcccg gcgcacacca acctgaccgc gccgcaaaac atcacgggcg
     1201 gaggcacgtt gaccagtacc agcgtggtct ggaccgtgag cggattgagc ggttcgggca
     1261 cctggtcggc caacttcgac atggggatct tggagcattg cggcgggccg cgggcgatcg
     1321 tatacaacac gatttacgcg acgacctaca ccgattgcaa gggctgtgaa cggaccgtta
     1381 ccggttcggg agagcggtac cccgtccaag tgcgtgccgg catcggttgc gaaacaaacg
     1441 cgtgcagctt tagcagtgcc aagttcatct ccacgaccga ggcggaagtt tgtgaagatg
     1501 tcatcatcac ccacgttttc agctcgttca ccggttccct gacggactgg acgggagtga
     1561 ctttctttgc cgatttggcg gagaacggct ggacagacac caatgcggtc cgggtatgga
     1621 ttgattcgac ggatgtcact agttatgtgg cgatctcgac caacgcctcc gggatgacaa
     1681 ttggcctgag cggtttgaac ggctcgccct atcccgacat ggcctcggtg accagcaacc
     1741 tccgcgtcat ttatcaggtg gcgtcagcct ccgccggccg gcagtatgat tattcgtatc
     1801 tttccacgcc gcaatgcgga acggaggaag acattgtcag ttggctgaat ggcgccgggc
     1861 gcatggccat taccctcaag ccggtcatga ttgcgggcgc ttgcgccacg acggatggag
     1921 agattgatct ggcgattcta cccttggatg gcgacacgct ctatccggtc cgggatgtgg
     1981 tggtcacgct gaacctggat tatgacggca acaacttctc cgctttccat tatgaaccgg
     2041 gttctaccgt gttccacaac attgtggatc cggcgggcaa tccgattacc gccagcgatc
     2101 cgacgatcag tccttccggc taccaatgga catggcactt gggcgatgtg ggcaccaaca
     2161 actatgccaa catgtccatt acctacaaga ttcacatggg ctgcacgaac gacaccagtg
     2221 cccagcaccg ggcggaagtg cgctataacg acaattgcag tctcggccaa gatccaccgg
     2281 cgcgggtggc cgagtccggc atcaacgggc cgccgctgtt cggcacgccg gtgctgttcg
     2341 tcaacttgca acctgaaatg caattcctgg ttgacacgca gatcgtcaac aaaatcctcg
     2401 tgatgaacac ctgtgcgata gaggccaaca acttgcgcgt ggaaatcgcc ctgccgccca
     2461 acgtctcgtt tgccggcgcg cagatttcgc cttcaaccgt gacggcgacc aatgtggtct
     2521 gggacttcca cacgatcaac caggccttcg ggccgctgca agacatggac ggcgacggca
     2581 gcattgacga tttgccgccc cggcaagtct ttgagttctg ggtgaccaac aacgtggatt
     2641 actgcgcccc gttgacggag cttaacctgc gcgcgtcgta tggttgctat ggcgatgact
     2701 gccaagtgac gccatgggac actgccggct atgaaagcat ctccggttcg ctggtgaccc
     2761 gggcaacgtt cccagtgcag aatgagctgt gcggcaccaa cccggtggtg tacagcgtgc
     2821 gcaacagcgg tttgaccgtg gattatgacg tgattacccg ccaaaccctg ccggcgggca
     2881 tcagctatgt gggtggcagc tcgcaggtga gcatcaatgg cggcgcgctg gccccgatta
     2941 gcgatcccag cggcagcgga ttgcccggcg acccgctggt ttgggattcg tccttgatcc
     3001 cgctgctggc ggccatgccg cccaccaatg aagtggccat ccattacaac gtggccttgg
     3061 gctgcgaagc agtaagcggt gacaaccggt tcatcgccga aggcgtttat acggatttgt
     3121 gcgacaatat tttgaccaac cgcgaaatcg tgtcggtgct ggcccccaag gagccgcttt
     3181 tggtgatgac caaaaccgct tccatgcccg tggccgatgc ggatgaggaa gtggtgtaca
     3241 ccatcacgat cagccacgat cccaactcgg ctgcgccggt gccgtacctc aagctgaccg
     3301 atgtgttgcc gtccgcaatg gattatgtgg gcgcttcggt cctaccggac agcgtgacgg
     3361 gtgcggggcc cggcggccaa accctggagt ggagcgccac caacctgttg gcccaaatcg
     3421 gcgatggcct gccgccgttt gccttgaacg aaggccccat cacgatactg gtgacggcaa
     3481 aggtggtggc ttgcgaagct tccgtcatca atcaagccac gctggattat ggttgctcgg
     3541 cggcggatgc ctgcatccag atcacggcca acgcgtccat cgtcacctcc ccgcggctgc
     3601 aaccgccggg ggtggatggc gaattggtgc tggacacctg cggcggcacc aagacggtgc
     3661 tggtgacgaa tagcggcgct tcgacggcct tcctcttcaa ttacacggag tgggcgccgg
     3721 cgggttacgt cttcacgggc gcttcggtga cgggtgagtt caacgccagc agtgccgagc
     3781 tcattgtgag ttatagcggt actccccatg gctcggtcgc gcacattgat ttcagtacca
     3841 aggccagcag cggcgcgacg gacgccaagg acgatgccgg ggatggcatt gacaacctcg
     3901 acttgggcaa aaacagcgga tttgtggtga catggacgct gcgcagcacg ggcgaaaacc
     3961 tcgactgcct cgccgacccg accgacctcg atttcgagga tccggaacag ggggtgccga
     4021 gccttctggg caccagcaac cttgtgacgt ataccgacct gtgccataac cccggcgagg
     4081 tcacggggag taccagcacc tatccggaca ttccggacct ggacattgat ttgcagccca
     4141 acagcctgat tgtgaccaac ggcgaagtgg tcaacttcaa ggtcaccgtc atcaacgatt
     4201 ccgaaaccgt gaatgccgat ggcatttacg tgcgggtgtt gctgggtgag gggtggacga
     4261 atgtcacgta tgtgtcgagt aacatcgtct cgtccggggc gaacgccatg acgatggacc
     4321 aggtggggga cacgaacctc ctgttcaact tccccggcgt gatcctgaat ccgatcaacg
     4381 acatcatcga aatcaacttt accgcgcaag ccacgacgaa cggcggcgcc ctgaccgcgc
     4441 tggccgaagt ggtgggcgat tgcggcaata gcgccatcac cccgagttgc accttcacta
     4501 atacgctggg cggcaacccg ctggccaata ccatgcaggg gagcacgctg gggccggtga
     4561 acgggcagta ttactcgttt gatcaggaca gctttgcggc cgcgggcctg gagctgacca
     4621 agaccgtgcg ctacgatgaa gagccggcgt cggcggcggg caccgagcgc gacgcgcgcg
     4681 tgggcgaaga tctgatctac cgcatcgagg cgttctactt tggcggtacg ttcaccaatg
     4741 tcaccatttc cgattcgctg ccgctggggc tgcacttcgg cacgccgacg gtggcggcga
     4801 cttcaggcgg catcacgggc gggagctggg gtgccggcac cggcgtgttc acgctggcgc
     4861 caacggcgtt cagcgtgccg tcaagcatca cgctcgatat ccccgtggtg gtgtcgaaca
     4921 tgatgttcgt gcaggacggg gtggtgatca ccaacgtggc cacgacccac tttgaggtgg
     4981 atggcgtgac caactcggcg ccggaagcct cgaccgaggt ggaagtgctg gagcccaacc
     5041 tgaagattac caaggtggcg gacaagacca gcaacattca agccggtgac atcatcacgt
     5101 ttaccaatat cattgagcac acggacttga gccgcaccag cgcctatagc gtggtgttca
     5161 cggacgtgtt gccggcgggc cttcagttca atacgtgggt gacgcccgcg agcggccagg
     5221 tgttggggca gagcattacg gtggatagca cggacttgcc ggccctggcg gagttcaaga
     5281 cgaatgcccc gccgatcgcg atcgtcttta cggtactggt gcaggaccag atcatcggtt
     5341 caaccgcaac caacatttcg gtggcgacgt atgaatcgtt ggattccggc tccaagaacg
     5401 gcaacgaacg tgacggctcg gacggccccg gcgggttgaa cgactactac acggacgatc
     5461 cggaagtatt gaactttgag gatttgcgcg gaatcaacaa gacgttcgtc tcgtcaagcc
     5521 agcagaacac ccatgattat gcaaccagca atgactggac gatcggcgag cggtttattt
     5581 accaaatccg cgtggacatg ccgcagggtg tggtgacgaa cctcgtgctg acggatacgg
     5641 tgccggtagg cattgacttc gtgggcacca acccggacgc cgcgctggcg tatccgggca
     5701 agggatatga gtttgaaatc ccggcgggcg gaccgcaatt tgtggaagcc aatgtggtgg
     5761 tgacggatcc ggatccatcg ccggcctcgt cggtggatac gaacggctcc gggcgggcga
     5821 tcaccttcac gttcccggcc atcaccaacg cggcggatgg caacgcgacc aacgactact
     5881 tcctgctgcg gatggaattc gtggcgctgg atatgcccta taacgcgggg ctgaggccga
     5941 atccgcgcat gggcagcaat gtggtgagcg tgacggacgg cacgaccacg ctgggcgcga
     6001 cggggccggt gcatcggatt gtggagcatg acatccgcgt gagtaagagc cgtacaccgt
     6061 tagagggcga tgcgggcgac ccgattacct tcacaattgt ggcctcgaac catgtgaacg
     6121 ctcttgccaa tgcctacaag gtggtggtgc gggactactt gaccaattcg gtgtatgacg
     6181 tgacgtcgtt cacatctctc tccacaccgc cgggctggac gctgaattgg cagaatcacg
     6241 gcacctacgg cctctatgag ctgtccgcga acgagggcgt gggattggca ccgggaacgg
     6301 gcgttagcgc tcagttcagc atcaacctgg cgcagacggt tcgtccgaac caggtttatc
     6361 agaacacggt gacgattccg cagagttcga cgatctcgaa cgatgtgccc accggcatca
     6421 aggaccgcca ggatggcgcg acgaacatgg tgccgcacgg cataccgggc atgagcgtca
     6481 gcaagattct ggaagcgacg agcgagacga acaatccgcc ggattcgacc ggttccaatg
     6541 tgcagatcgg cgaagtggtg acttaccgca tcggcgtcac catgccggaa tccaccatca
     6601 cgaacttaag cgtggtggac catgtgtcca ataacgggtt ggcctacatc tatgggtcgg
     6661 caagactgga cactggtgcg tggggcccga atgccgcgtt tgtgggcaac actggcaact
     6721 tcacggagaa cccatccggg gcgttgggcg atctggcgcc actggggcag gccatcacct
     6781 tcgggttcac caacgtggtc gtaacgggcg acaacaatac aacgaacaac acgttctaca
     6841 ttctggcgga ctatctggtg cgtagcaacg cggtgaacaa cgggttgggc ccgaacccga
     6901 cggtgcacac caaccatgcc acgctgacct atcagggtaa cccggggaat accgtgacct
     6961 ccgccgtggt gacaactacc gtgatcgaac caaaacttga gatcaccaag agcctgagtc
     7021 ccacgaacca cgtggacgcg ggcgacatca tttccgtgac gctggtggtg accaatagcg
     7081 gcacgggcac ggcctatgat gtggtgattg aagacgagct ggcgctgccg tatttcgata
     7141 tttcgacgct ggccaatgtc atcgtgccga cgggcttcgt gcacgaggtg gatggcgata
     7201 tcctgcgctt caagtcctac gagtttgcgc cgacgggtac gaacacgatt gaagccggcg
     7261 aaaccttgac ctttaccttt gacgtgcaca cgggcgccgg tttgccgccg aactccgtgg
     7321 tgaccaacac cgccacggtg aaaggcgact cgatcaagga taacggcccg aagaacgaac
     7381 agcgggacac caacgacgag gatgaagatc agttcacgtc cgatgatttc catcccacca
     7441 aggcgctcat tgccaccagc gaaacagggc cggtggattc cacggggaat aacctgcaaa
     7501 tcggtgaagt ggcgacgtat gagatcaagg tgaatctgcc ggaaggcgtc attcccgacc
     7561 tgaccatcac cgacgtgatg ccggcgggca tggcgtatgt ggtgggctcg gcgacggtgg
     7621 atgcctccga ggtgagtgga gccatgggcg cctttacggt cacgccgaat gtcggcgttt
     7681 tgggtgccag cggcgaaaat gtggtgatga cctggcaagg gaacagcgtg atcacggcat
     7741 ccaccaacca tagccacacg gccaatgtcc tgcgcgtgac gatgcaggcg gtgatgatgg
     7801 acctgcccgc caactcgggc ttgcctccgc agaccgtgtt gaccaacaag gccaccgtca
     7861 cctttaccgg caacccgaat ccgccggcgg aaattgacgg cccgccggtg acggccgtgg
     7921 agccggacat tgcgatcttc aagacggtct ccccgaccaa cggcgtggat gcgggcgatg
     7981 aaatcaccat cacgctggtg gccaccaaca gcggcaccgc caccgcctat gacctcgtga
     8041 tcagcgacgt actgagcgag gatatcttca atctggcgag cgtgacgaat gtcagcatgc
     8101 cggccgggtt tgtctttgcc accacgccgg gtgcaggcac gctgacggtg acctactcat
     8161 cggatccggc cagcagccag ccgaccaatt cactggaagt gggcgagagc cttgagttcg
     8221 tcttcacggt gcacgcgtcg caggcggtcg agccgaacag cggctacacc aacacggtca
     8281 gtttgcgcgc cgatacgatt gatggcacgc cgccgacccc ggaggagcgc gaatataccg
     8341 aagacgatga ggacgacttc gacacgccca agatgagcat tgccaaggtg ctgaccggca
     8401 cctcggagaa cggcccggcg gattcgacgg gcagcgatgt gcagatcggc gagaccgcga
     8461 cctaccgcct gacggtgacc ctgccggagg ccacgatcac gaacctgacg gtagtggacc
     8521 tggtgccggt gggcatggag tatgtggcgg gcagcgtcgc ggtggatacc aacggtttcg
     8581 gcggcacctt gccggccggc tcgccgactg tgacgacgct cggcggctcc ggagacgaca
     8641 tcacgatcgt cttcgagggc aacacggtgg tgacggataa taacgatccg agcgacaact
     8701 ccttcacgat taccttgacc ctgttgacgc tgaatgtggg ttccaacacc ggcaaggtgg
     8761 ccggagcgca gacggtgctg aacaaccggg ccaatgtgtc gtatgacggc aaggatccgg
     8821 aagacgagac cccggatgtg ccggtggtcg tgattgaacc ggtgctggcc atcgagaaga
     8881 ccatggcgct gatcgggctg gatgtggtcg aaatcaagct ggtggtcacc aacagcggtt
     8941 tggcgacggc attcgacgtg gaaattcagg acgtgctgac caccaactgg tgggataccg
     9001 acacgattac gaatgtgacc attccggatg gattcacctt cagcttcgcg ggcaacccgg
     9061 gcgacgcgac ggtgacaatc gcgagcgacc cggcatcagg ccaaccgacc aactcgattg
     9121 aggtcggcga agcactggaa ttcgtgttcc gcgcgacatt gatccctgat gctccgagcc
     9181 cggtgaccaa ccgcgccact gtgacggaat acagctcgaa ggacggcaag gatgatgacg
     9241 agcgcaagta tccggatgaa gacgacgaga aggaactgcc gctgccgaag tacaccctga
     9301 ccaagacact gaccagtccg ttgggccgcc cggcggtggt gggcgagacg gtcaccttca
     9361 ccatcgaggt ggcgaacacc ggcgacgtgg gccttggctt ggtgccgctg gatgacacct
     9421 atgatgcgac gtacctgagt ttccagtcag cgtcgccggc ggaaaccagc acaggcccgg
     9481 gcagcatcca ctgggatgac atcgggccga ttggggtagg cagttccaag gtggtgacgg
     9541 tggacttcac agccatcgaa agcacgctcc cgagcgacac gaccaactgg gtgacgacca
     9601 gtccgttcac cacgaacgat attccgctga agcccaagac caacgaggcg ccggtggaag
     9661 tgcgttacgc tggctttacg ctggagaaaa cgcgcaccag ccccgcgggc cgcgccgcgc
     9721 agataggcga ggaagttgtg ttcaccatca cagtggtcaa tacgggtgag gtgaacctgg
     9781 tgacggttcc gctggaagac acgtatgaga cggcattctt gagctatatc gagtcggaac
     9841 cggcctcgga tgacaacaat aacgacggcg tgatcaactg ggccaacatt ggtccgctgg
     9901 caatgggcga gacggccgta gtcacatccc gcttcaccgc ggtggctacg acgaccaacc
     9961 agtcgcgtac caacatcgtg gtggcatcgc cgaccacgac caacacgccg ccactgccgc
    10021 cgcagaccaa tccggcgccc tacgagatcg tgaacgcgga ctataccttg atcaaggaag
    10081 tggtcagccc catcggccgc gcggcggaag tgggcgaaac cgtggtgttc cgcctgacgg
    10141 tggagaatac cggcgaggcg ccgttggtga cggtaccggt agaggacacc tacgagacgg
    10201 cgtatctgga atatgtctcg tcagtgccgg catcggatga caatgtgaac gatggcgtga
    10261 tcaactgggc ggatgtcggg ccgatagcgg ttggcgacag tgtctcgatt gatgtgacct
    10321 tcacggcggt ggcgtccacg gggggcgaac agaagaccaa cacggtgaca acggctccga
    10381 caacgccgcc agattatcca gacgtgccgc cgaagaccaa tgaagtaccg tacgaggtgc
    10441 aaatcgtcgg ttacacgctg gtgaaggaag tcacgagtcc gctgggtcgc ccggccaaca
    10501 tcggcgaaga agtggtgttc accatcacgg tggccaacac gggcgaagtc gctttggtga
    10561 cggtgccggt ggaggatacc tacgagacgg cgtacttgag ctacgtctcg tcgctgccgg
    10621 catcagatga caacattaat gacggcgtga tcaactgggc gaacattggg ccgatccccg
    10681 tgggcgagag ccgctcaatt gtcgcaacct ttacggcggt ggcttcgacc accaacagtt
    10741 cacgcaccaa cattgtagtg acggctcccg agacgcctcc ggagtcgcct ccggtgcctc
    10801 cgaagaccaa cgaagtgccg tacgaggtac tcagctccga ctacatcctg accaaggaaa
    10861 ttgtcagccc gacgaaccgc gctgcggaag tcggcgaaac cgtggtcttc cgcctgacgg
    10921 tggagaacac gggcgaggtg gaactggtga ccgtgccggt tgaagacacc tatgagacgg
    10981 cgtatctgga atacgtttcg tcggtgccgg catcggacga caacgtgaat gacggcgtta
    11041 tcaactggac ggatatcggt ccgattcccg ttggcgagag ccgttcaatt ttggcgacct
    11101 tcacggcggt ggcatccacg gagggcgact ccaaaaccaa tagagtggtg acggctccga
    11161 caacacctcc gaatacgcct ccggtgccgc cgaagaccaa cgaagtgccg tacgaggtgc
    11221 aaatcgtcgg ttacacgctg gtgaaggaag tcacgagccc ggtggggcgt gccgccaaca
    11281 tcggcgagga catcgtgttc accatcacgg tggccaacac gggcgaagtc gctttggtga
    11341 cggtgccggt ggaagacacc tacgagacgg catatctgga atatgtctcg tcggtgccgg
    11401 cgtcggatga caacatcaat gacggcgtga tcaactgggc gaacattggg ccgattccag
    11461 ttggtggcag cgcatccatt gtggcgacct tcaccgctgt tgcctcgagc accaacctgg
    11521 cgcgcaccaa catcgtggtg acggctccgg agacgccgac gaattcgccg ccggtgcctc
    11581 cgaagacaaa tgacgtgccg tatcagattg tgtcggcctc ctacaagctg ctgaaagagc
    11641 ggatcagacc ggcggaccgt cccgccatca tcggcgagga agtggtcttc cggattacgg
    11701 tcgaaaacga cggcgaagcg gatctggtgg aagttccggt ggaagaccgg tatgagacga
    11761 catacttgag ctacgttggg tcggagccgg cctctgacga caacattgac gatggcgtga
    11821 tcaactggac gaatattgga ccgttgccgg cgggcgcgac cacctcgatt gtggcgactt
    11881 tcacggcggt gctgaacacg accgaagtcc acaccaactg ggtggagacg gctcccgaga
    11941 cgcctccggg atatccgccg gtgccgccgc agaccaatcc cgccccctac gaggtggata
    12001 ttcccgcgcg tttgggtgac cgggtctggg aggacttgaa cggcgacggc atccaggatg
    12061 ccggcgagcc gggtatcccg ggtgtgaagg tgacactgtt tggcacaaac ggcagcgtgg
    12121 tggcgacaac cgtcacggac gtg
//
LOCUS       DAOPNP010000074        12012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6140_length_12012_cov_9.120348, whole genome shotgun sequence.
ACCESSION   DAOPNP010000074 DAOPNP010000000
VERSION     DAOPNP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12012
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6140_length_12012_cov_9.120348"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1760
                     /locus_tag="PKI64_07560"
     CDS             402..1760
                     /locus_tag="PKI64_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00901.1"
                     /translation="MGGMMAAGGAWLWRRARGCLLAGFWLLVLMLASVPAVADDVAVK
                     IGNPLLAKFPVGGDARPRSIWDLQVFNDKLYLAHGDYWNNAGPIDVWTYQGDGTNFVK
                     EYTTQEEMIWDFFVQDDLLFIPGVDSVEESPIRANIYVQDPQNGPGGGWTRRATLTGG
                     VHSYDVALFQNRLFTSMTMENQQARTLVSTDMGASWSVLLQQYSAPIPFADFLFLQGP
                     TNYVYDGSQLTAVTPQLHVSQLSMARRVRVGDGLLYAYPVRYLLTPTPLYYMTAAQIQ
                     ASGAANQLMAFAGTCVRDILAHDGLCYVMTAETNTTSTFRGRIYASADLTHWWQASEF
                     TVPGVPLSFELYRNRFYVGLGSLFTGNWDKLVGPEAGSIWRIQPAAVEMAWVPAKEGV
                     GGSMTVLGAAGLTLTVEATSSLQDPQWEVIGTVQSASGIADLVDPDAAAHEARYYRVI
                     LE"
     gene            1850..2869
                     /gene="ruvB"
                     /locus_tag="PKI64_07565"
     CDS             1850..2869
                     /gene="ruvB"
                     /locus_tag="PKI64_07565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOE00902.1"
                     /translation="MSDRFASETLNRADVEFEQTLRPGRFVDFVGQGKVRERLELSVA
                     AARGRGESLDHVLLCGPPGLGKTTMAYILGAAMGVNVKATSGPILAKPADLAGLLTNL
                     EKGEILFIDEIHRIQKSVEEYLYSAMEDFVIDIVLDQGAHARSVRLNLPRFTLVGATT
                     RSGLLSAPMRSRFGLINRLDYYSAEELYAVVMRSAEILGVNIADDGAQEIAARARGTP
                     RIANNLLRWVRDYAQVRAGNRITRDVADRALAMLDVDRHGLDEMDKRILATIIEKYGG
                     GPVGLATVAVSVGEEPGTLEEVYEPYLIQQGLLARTPQGRTATLTAYQLLGLRPPAGF
                     QQELL"
     gene            2869..3876
                     /locus_tag="PKI64_07570"
     CDS             2869..3876
                     /locus_tag="PKI64_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOE00903.1"
                     /translation="MNILVTGAAGFIGYHVAQALLARGDTVTGFDSINDYYDVTLKEA
                     RVARLRPQPGFTFVKGSLEDAAAVRALFAGSPFDRVIHLAAQAGVRYSLDHPEAYIQS
                     NIVGFLNILEACRHARTPHLAYASSSSVYGLNTQQPFSVHDNVDHPISLYAATKKANE
                     LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGQPIEVYNHGKMRRDF
                     TYIDDIVQGVMRVSDRIAQPNPAWDAARPDPGTSPAPFRVYNIGNHSPVELDEFIATI
                     EAALGKRAIRRDLPMQPGDVPATCADVADLERDVGFAPATPLATGIARFIEWYREYYG
                     V"
     gene            4043..5329
                     /locus_tag="PKI64_07575"
     CDS             4043..5329
                     /locus_tag="PKI64_07575"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOE00904.1"
                     /translation="MANTILIGAQWGDEGKGKIIDVLTERVDWVVRAQGGNNAGHTVV
                     VGGERIVLHLVPSGILHAGKRCVIGNGVVLDPMALVAEIAEVTRRGHEVAGRLFISDR
                     AHVVFPYHRMLDAQGEARRAKGEQIGTTQRGIGPTYSDKAARFGLRMCDLLDPDFPAL
                     LRARVEEKNRVLAAMGAPVADYAAVLADIQQAMTTLAPLVCDTIPLLHDAVQRGERIL
                     FEGAQGIMLDIDFGTYPFVTSSNASSGGAATGSGVPPTRMNNVIGVLKAYTTRVGEGP
                     FPTELHDANGDEIRRVGNEFGATTGRPRRTGWFDAVVARHAVMTCGVTEWALTKLDVL
                     DGFATIPICVAYELDGQRINHFPANIRAAARCRPVYEEMPGWHTPTRGITNFADLPDA
                     AKRYVARIVQLTGVPVGILSLGPGRESTLLLGAQPA"
     gene            5326..6045
                     /locus_tag="PKI64_07580"
     CDS             5326..6045
                     /locus_tag="PKI64_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOE00905.1"
                     /translation="MSPAATPAIPRHVAIIMDGNGRWAKQRRKPRLFGHRAGADSLRA
                     VLRACRDHGVEYLTVYAFSTENWVRPKQEVAGLMSLLKTFLKKDEHELHENQVRLRMT
                     GRMGDLPAAVRAELERVMVATRHYTKGHLILALSYGGRAEIVDAARALAADIQAGRSR
                     PEDISEKTFATRLYLPDVPDPDLVIRTSGEIRLSNFLLWESAYSEFYFTETLWPDFRE
                     ADFAKALAEYARRQRRFGAPS"
     gene            6042..>6418
                     /locus_tag="PKI64_07585"
     CDS             6042..>6418
                     /locus_tag="PKI64_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00906.1"
                     /translation="MSLPNIRHRLLSGFTIAGVLFAAFFFLPDAGAPFLLAALSAMIA
                     LEFYQLMTAGGVANFRYYGTVGCVALVFATWHVGRQGDAGSWDALVLFLITLGIFIRQ
                     FPQKNNPHPLRTIGGTLFGVLYVG"
     assembly_gap    6419..6518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6519..6707
                     /locus_tag="PKI64_07590"
     CDS             <6519..6707
                     /locus_tag="PKI64_07590"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE00907.1"
                     /translation="PLGVGLAVCAIVGDLVESLFKRAANVKDTGGVIPGMGGLLDVLD
                     SILFTAPALYLVLRLLQA"
     gene            6747..8195
                     /gene="rseP"
                     /locus_tag="PKI64_07595"
     CDS             6747..8195
                     /gene="rseP"
                     /locus_tag="PKI64_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOE00908.1"
                     /translation="MALLYILAMILLFSVSIFVHELGHFLAARACGMVADVFSIGMGP
                     ALWKRKIGATTYKIGCLPIGGYVALPQMDPEAFLARAAARDGEDNDAPPAPPLPRIAP
                     GKKILVAMAGAAGNVILAFVLATLVWVVGKPASLAERNSVVGFIATNSPARAAGLVVG
                     DVITTVNDQPVGNWLQIMEAVALAGRPTVTLRIQPAAGDSARELSLPAAKNEVGMWEL
                     PDIAGQTPCHVAAVYPNSGALAAGLRAGDQLLRFDGQTIHSQGHLSQLVEAAAGRAAT
                     IEFLRDGVIHTASVTSTYDAELHRHLIGIQFNTLADLDYTTRVHPTPWAQVRDHAGGI
                     FRFLSALTTPATSGAAAGAVGGPVLILVMLWLMVKSSFILAIWFTGFLNVNLAIINLL
                     PLPLLDGGHVIFNLWEMATRRPVPAKWIHALTNLFVVLFIGVFLLLVYRDSVRHIIPG
                     LHRWWTDAPDPAPHVFQTPPGATEDSPSASTP"
     gene            complement(8222..9772)
                     /locus_tag="PKI64_07600"
     CDS             complement(8222..9772)
                     /locus_tag="PKI64_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00909.1"
                     /translation="MKRSTAVLLAVVLWGGQSVAGELVWQTFDNVATGIISTVPGWIA
                     TPTQPTVSAANAYSAPHALEIATPAGGSSAAYTNFATIYSILLGTEHPVIRVSAKLFV
                     PHTNVNLSFGLKDTLSNREFNFVCTNGVAKLHNSPVSFPLVTGRFVDVTFHLNRSNGA
                     TRLYYDVTNIVAWGTYNAGTNYTRFDRFVVTRTKSPSDGDSVFLDNIRVDTFPPYVWA
                     WWRFDEDSPSYQDHVGAFGVTNNRPINLTPTDPAYAYRPLYDGQTEYSNEVVSRRSSS
                     SYLPCEVPTPTMTDWTVEFIFQHDPTETAHELFDWGSDLGFNSTSAYVALTYHTNATG
                     FSAEFRDSQQTAAGYHWLSKTFRYQPDARWHHVAFIKSSTNLHIYFDYRLAHTTNLDS
                     RARGAYRFDTNSRVRLGMALNGANPSDEYTYFDEVRFSTKALDLREFLQPTRPIILSC
                     DNSALNPLWELTLKTIKGREYRTEAASSPTNATWSSLGTFIAGDPVHSIQVPTTPGNR
                     FVRIRPLP"
     gene            complement(9878..11902)
                     /locus_tag="PKI64_07605"
     CDS             complement(9878..11902)
                     /locus_tag="PKI64_07605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE00910.1"
                     /translation="MRTPRPKSQTPKSPNHIFCLAQADKPKPAGLERKTKGVACYYAC
                     GQHETMTRKHSSWRGALLVFGVAIVLRAAFLLEASTKPAFSLVYMDPEYNLEWARGMA
                     TGVWNPPYDTLREGPFFRAPLYSMFLAAIWGVFGEKLWIFRSFQAVLGSFSCVLVFLL
                     AKRLYDQRVGILAGLLCACYWVLIYFDSQFLFPVLLVFLVLAGFLALIWSLDRKNMMF
                     GAISGLFFGLFAITRPNILVFFPFLALWGLWAWPGTFKKQILFAFWLAFGAGLPPLAA
                     TVYNGVVGDDWVLVASQGGVNFYIGNNPQSNGMQAVVPGTRASWWGGREDTIAIAEQA
                     AGRLLKPSEVSSYWYAKSLDYIREQPVEWLKLTLRKAIAMFGDVEIPNNAPYQARRGE
                     FFTLSAIPLGFAAIFALFLVSTPWILPKKSDFEAQNTARSVILLILVFLATYSASIIA
                     FFVTGRYRMPLVPFFAMGAAVGIVRAHDFIRARQWRSTTALVAVFLLLFGVLKMDFFK
                     IREQTAEFTRLTIAQDHMQLGEWDEAIQQLEAIRQTRRLSMVEVYISLARAYLHRGSP
                     NDAAAILAVAEEGLQLHPSTPELLWYAAVGRVQMADWPHARLWIEQYVLAEPGDVRGL
                     HLAHLVATQQNRHADAQEALRRAQSIDPHHPAVRDMLKASGMGGNTPPFH"
BASE COUNT         2376 a         3614 c         3599 g         2323 t
ORIGIN      
        1 ttcaggttct tatgccctta aaagcgtgtg ggttcgagtc cctccgggtg caccactttt
       61 tttgtccgca cgcggaccca accggcccgc gtgacgcttt tttgcggtgc gcgaactgag
      121 ttttccacag tgtggaaaag ttgggccaaa gttgttccat tgcgtggaaa aatttcccaa
      181 agttgttcca ttgtgtggaa aagtgaggtc aaagtttgcc gctgcgttcg ctatggctca
      241 ctggctcgtc cggctgcgcc ggccgctata gaactcgcgt tcgcccatcg cccttcgggc
      301 tcgggctcgg cactcgtcta ttttccattg cgtggaaaaa tcgtgccgcg tttttccacg
      361 gtgtggaaaa aagactttgt gcgcggacaa agttgggtaa cgtgggcggg atgatggcgg
      421 caggcggagc atggttatgg cgtcgggcgc gggggtgcct gctggcggga ttctggctgc
      481 tggtgctgat gctggcgagc gtaccggcgg tggccgacga tgtcgccgta aaaattggca
      541 acccgctgct ggccaaattt ccggtggggg gggatgcccg cccgcgatcc atctgggatt
      601 tgcaggtgtt taacgacaag ctctaccttg cgcacggcga ctactggaac aacgccggcc
      661 cgattgatgt gtggacttac caaggcgatg gcaccaactt cgtcaaggag tacaccacgc
      721 aagaggaaat gatttgggat ttttttgtgc aggatgatct cctgtttatt ccgggggtgg
      781 actcggtgga ggaaagcccc atccgcgcca atatttatgt gcaggacccg caaaacgggc
      841 cgggcggtgg gtggacgcgt cgagccacgc ttaccggcgg cgtgcactcc tatgatgtgg
      901 cgctgtttca gaatcgcttg ttcaccagca tgaccatgga aaaccagcag gcgcgcacgc
      961 tggtttccac cgacatgggc gccagttggt cggtgttgct ccaacaatac agcgcgccga
     1021 ttccgttcgc ggactttttg tttctgcagg ggccgacgaa ttatgtgtac gacggcagcc
     1081 agttgacggc agtgactccg cagttgcacg tcagccagtt gtccatggcc cgccgcgtgc
     1141 gggtgggcga cggcctgctc tatgcctacc ccgtgcgcta tctgctcact ccaacgccgc
     1201 tgtactacat gacggccgcg cagatccaag cgagcggcgc ggcaaaccag ttgatggctt
     1261 tcgcggggac ttgcgtgcgc gatattctgg cgcacgacgg cctgtgctac gtgatgacgg
     1321 cagagacgaa tacgaccagc acgttccgcg gccggattta tgcgtcggcc gatctcaccc
     1381 actggtggca ggccagcgag ttcaccgtgc cgggggtgcc gctctcgttt gagctgtacc
     1441 gcaaccggtt ttatgtgggg ctggggagtc tgttcactgg caattgggac aagttggtgg
     1501 ggccggaagc gggcagcatc tggcgtatcc agcctgccgc cgtggaaatg gcgtgggtgc
     1561 ccgcgaaaga gggcgtgggc ggctccatga cggtgctggg cgcggcggga ctcacgctga
     1621 cggtggaagc cacatcctcg ctgcaagacc cgcaatggga ggtcatcggc acagtgcaat
     1681 cggccagtgg catcgcggat ttggtggatc ccgacgcggc ggcacatgaa gcccgctatt
     1741 accgcgtcat tctggaataa cccggaccgg gtgcgcgcgg cgggggtttg ccggtgcttg
     1801 tcaggcgggg ggctttctgg tttagtggcc ggactggcac gaacaacgca tgagtgaccg
     1861 ttttgcatct gaaaccctga accgcgcgga cgtggaattt gaacagacgc tgcggccggg
     1921 acgctttgtg gattttgtcg gccaaggcaa ggtgcgcgag cggctggagt tgagcgtggc
     1981 ggcggcgcgg ggacgagggg agtcgctgga tcacgtgctg ctgtgcgggc cgccgggcct
     2041 cggcaagacc accatggcct acatcctcgg ggcggcgatg ggcgtcaacg tcaaggctac
     2101 ctccgggccc attctggcca agccggccga cttggccggg ctgctgacca accttgaaaa
     2161 aggcgaaatc cttttcattg acgaaatcca ccgcatccaa aaaagcgtgg aggaatatct
     2221 ctactcggcg atggaagact ttgtgattga tattgtgctg gaccagggcg cccacgcgcg
     2281 cagcgtgcgc ttgaacctgc cgcgctttac gctggtgggg gccaccacgc gcagcggcct
     2341 gctcagcgcg cccatgcgct cgcgcttcgg cctgatcaac cgcctcgatt attactcggc
     2401 agaggagttg tatgccgtag tcatgcggtc ggcggaaatc ctcggggtga acattgccga
     2461 cgacggcgct caggagattg ccgcgcgcgc ccgcggcacg ccccgcatcg ccaacaacct
     2521 gctgcgctgg gtgcgtgact acgcccaagt gcgcgccggc aaccgcatca cacgcgacgt
     2581 ggccgaccgc gcgctggcca tgctggatgt ggaccgccac ggcctcgacg aaatggacaa
     2641 acgcatcttg gccaccatca ttgaaaagta tggcggcggg ccggtgggcc tcgccaccgt
     2701 ggcggtgtcc gtgggtgaag aaccgggcac gctggaagaa gtgtatgagc catatctgat
     2761 ccaacagggc ctgctggcgc gcacgccgca ggggcgcacc gcgacgctga cggcctacca
     2821 gttgctgggc ttgcgtccgc cggcgggctt tcaacaggag ttgctctgat gaatatcctc
     2881 gtgaccggcg cggccggctt cattggctac cacgtggcgc aggcgctgtt ggcgcgcggc
     2941 gacaccgtaa ccggctttga ttccatcaac gattattacg acgtgacgct caaggaggcg
     3001 cgcgtggcgc ggctgcgccc gcagccgggc tttacgtttg tgaagggcag cctcgaagac
     3061 gccgcggcgg tgcgcgcgct gtttgccggg agccccttcg accgcgtaat ccatttggcc
     3121 gcgcaggcag gcgtgcgcta ttcactggat catcccgagg cctacatcca gagcaacatc
     3181 gtcggctttt tgaacattct ggaagcgtgc cggcacgcgc ggacgccgca tctggcctat
     3241 gcctccagca gctcggtcta tggcctcaac acgcagcagc cattttcagt gcacgacaac
     3301 gtggaccacc ccatctcgct ctacgcggcc accaaaaagg ccaacgagct gatggcccac
     3361 acctactcgc acctctacgg gctgcctacc accggcctgc gcttcttcac cgtctatggc
     3421 ccgtggggcc gccccgacat ggcgttgttc aaattcacca aggccattct ggccggccag
     3481 cccattgagg tttataatca cgggaagatg cgccgcgatt ttacgtacat tgatgacatt
     3541 gtgcagggcg tgatgcgcgt gagcgaccgc atcgcccagc caaatcccgc gtgggatgcc
     3601 gcccgcccgg acccgggcac cagccccgcg ccattccggg tgtataacat cggcaaccat
     3661 tcgccggtgg agttggacga atttatcgcc accatcgaag ccgcgctggg caagcgggcc
     3721 attcgccgcg atttgcccat gcagcccggc gatgtccccg ccacttgcgc cgacgtggcc
     3781 gacctcgagc gcgacgtggg ctttgccccc gccacccccc tcgccaccgg catcgcgcgg
     3841 tttattgagt ggtatcgtga atattatggg gtctaggagt gtaggagtct aggagtgtag
     3901 gagtcgagga gtgtaggagc cgaggagtgc agaatgaaca acccccaaaa taaatttacg
     3961 ctactcatcc acgctggcgc gcttctggaa tccgctgctc ccaaactccc atactcctaa
     4021 tactcccgaa aggatctccg ccatggccaa caccattttg attggcgccc agtggggcga
     4081 tgagggcaaa ggcaaaatta ttgacgtgct gaccgagcgc gtggattggg tggtgcgcgc
     4141 gcagggcggc aacaatgccg gccacacggt ggtggtgggg ggcgagcgga tcgtgctgca
     4201 tctggtgccc tcagggattt tgcacgccgg caagcgctgc gtaatcggca acggcgtggt
     4261 gctcgacccc atggcgctcg tggccgaaat cgcggaagtg acccgccgcg ggcacgaagt
     4321 ggccgggcgg ctgttcatta gcgaccgcgc gcacgtggtg ttcccctatc accggatgct
     4381 cgatgcccaa ggcgaggcgc gtcgcgccaa gggcgagcaa atcggcacca cgcagcgcgg
     4441 catcggcccg acctattctg ataaggccgc gcgcttcggc ctgcggatgt gcgatttgct
     4501 cgatccggat ttccccgcgc tgctgcgcgc ccgcgtcgaa gagaaaaacc gcgtgctggc
     4561 tgccatgggc gcgcccgtcg ccgactacgc ggctgtcctc gccgatatcc agcaggccat
     4621 gaccaccctc gcgccgctgg tgtgcgacac catccccctg ctgcacgatg ccgtccagcg
     4681 cggcgaacgc atcttgttcg agggcgcgca gggcatcatg cttgatattg atttcggcac
     4741 gtatccgttt gtcaccagct ccaacgcgtc cagcggcggt gcggccactg gctccggcgt
     4801 gccgcccacc cgcatgaaca acgtcattgg cgtgctgaag gcctatacca cgcgcgtcgg
     4861 cgagggcccg ttccccaccg aactgcacga tgccaacggc gacgaaatcc gccgcgtggg
     4921 caacgagttc ggcgccacca ctggccgccc gcgccgcacc ggctggttcg atgccgtcgt
     4981 ggcccgccac gccgtcatga cttgcggcgt gaccgaatgg gcgctcacca agctcgacgt
     5041 gctcgatggc tttgccacca tccccatttg tgtggcctac gagctcgatg gccagcgcat
     5101 caaccatttt ccggccaaca tccgcgccgc cgcgcgctgc cgccccgttt atgaagaaat
     5161 gcccggctgg cacaccccca cgcgcggcat caccaacttt gccgacctgc ccgatgccgc
     5221 caaacgctac gtggcgcgga tcgtgcaact gaccggcgtg cccgtgggca tcctctcgct
     5281 gggcccgggc cgtgaaagca ccctgctgct gggggcgcaa cccgcatgag tccggccgcc
     5341 actcccgcca tcccgcgcca tgtggccatc atcatggatg gcaacggacg ctgggccaag
     5401 cagcggcgca agccccgcct cttcggacat cgcgcgggcg cggattcgct gcgcgccgtg
     5461 ctgcgcgcct gccgcgacca cggcgtggaa tacctgacgg tctatgcttt cagcacggaa
     5521 aactgggtgc gccccaagca ggaagtcgcc ggcctgatgt cgttgctgaa aacttttctg
     5581 aaaaaagacg aacacgagct gcacgaaaac caggtgcgcc tgcgcatgac cggccgcatg
     5641 ggcgatttgc cggccgccgt gcgcgccgag ttggagcgcg tgatggtcgc cacccggcac
     5701 tataccaaag ggcatttgat tctggccttg agctacggcg ggcgcgccga aatcgtggat
     5761 gccgcgcgcg cgctggcggc cgatatccaa gccggacgct cgcgcccgga ggacatcagc
     5821 gagaaaacct tcgccactcg cctctacctg cccgacgtgc ccgaccccga tctggtcatc
     5881 cgcaccagcg gcgaaatccg tctgagcaat ttcctgctct gggagtcggc ctattccgaa
     5941 ttctatttca cggaaacctt gtggccggat tttcgcgaag ccgacttcgc caaagcgctg
     6001 gctgaatacg cgcggcggca gcggcgcttt ggagccccct catgagtttg cccaatatcc
     6061 gccatcgttt actgagcggt ttcaccatcg ccggcgtgtt attcgccgcg ttctttttcc
     6121 tgcccgatgc cggcgcaccg tttctgctgg cggcgctctc tgccatgatc gcgctggagt
     6181 tttaccaact gatgaccgcc ggaggcgtgg ccaacttccg ctattacggc actgtcggct
     6241 gcgtggcgct ggtgtttgcc acctggcacg tgggccggca aggcgatgcc ggcagttggg
     6301 atgcgctggt gctgttcctc atcacactgg gcatttttat tcggcagttc ccgcagaaaa
     6361 ataatccgca cccattgcgc acgatcggcg gcacgctgtt cggcgtgctg tatgtcggnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gctgggcgtc ggcttggcgg
     6541 tttgcgccat cgtgggcgat ttggtggaat cgctcttcaa gcgcgccgcc aatgtcaagg
     6601 ataccggcgg cgtcattccg ggcatgggcg gcttgctcga cgtgctcgac agcattcttt
     6661 ttaccgcgcc tgccctctat ttggtgctgc gcttgctcca ggcctgaccg ttgacccgcc
     6721 acccctaatc accccgcgca atccccatgg ctcttctcta tattttggcg atgatcctgc
     6781 tcttcagcgt gagcattttt gttcacgagc tgggccattt tttggccgcg cgcgcctgcg
     6841 gcatggtggc cgatgtgttt tccatcggca tgggcccggc gctgtggaag cgtaaaatcg
     6901 gcgccaccac ctacaagatc ggctgcctgc ccatcggcgg ctatgtcgcc ctgccgcaaa
     6961 tggaccccga ggctttcctg gcccgcgcgg ccgcgcgcga tggcgaggac aacgacgccc
     7021 cccccgcgcc tccactgccg cgcattgcgc ccggcaaaaa aatcctggtg gccatggccg
     7081 gcgctgccgg caacgtgatc ctggcgtttg tgctggccac gctggtttgg gtggtgggca
     7141 agccggcctc gttggcggag cgcaacagcg tggtgggttt catcgccacc aacagcccgg
     7201 cccgcgccgc cggccttgtc gtgggcgatg tcatcaccac cgtcaacgat cagcccgtcg
     7261 gcaattggct gcaaatcatg gaagctgtcg ccttggccgg ccgtccgacc gtcacgctcc
     7321 gcatccaacc cgccgccggc gacagcgcgc gcgagttgag cctgcccgcc gccaaaaacg
     7381 aagtcggcat gtgggagttg cccgacatcg ccgggcaaac gccgtgccac gttgccgcgg
     7441 tatatcccaa ttctggcgcg ttggccgccg gcctgcgcgc cggcgatcag ctcctgcgct
     7501 tcgatgggca aaccatccac agccaaggcc atttgagcca gttggttgag gccgccgccg
     7561 gccgcgccgc caccatcgag tttctgcgcg atggcgtcat ccacactgcc agcgtcactt
     7621 ccacctacga tgccgagctc caccgccatc tcattggcat ccaattcaac acgctggcgg
     7681 atttggacta caccacgcgc gtgcatccca cgccgtgggc gcaggtgcgc gaccacgccg
     7741 gtggcatctt ccgttttttg agcgcgctga ccacgcccgc cacttccggc gccgcagccg
     7801 gtgcggtggg cggccccgtg ctgattctcg tgatgctctg gctaatggtc aaatccagct
     7861 ttattctcgc catctggttc acgggatttc tcaacgtcaa cctggccatc atcaatctgc
     7921 tgcccctccc gctgctggat ggcgggcacg tgattttcaa cctttgggaa atggccaccc
     7981 gccgccccgt gcccgccaaa tggattcacg ccctgacgaa cctttttgtg gtgctgttca
     8041 tcggcgtgtt tctcctgctg gtctatcgcg attccgtccg ccacatcatc cccggcctgc
     8101 accgctggtg gaccgatgcc cccgaccctg cgccccacgt cttccagacg ccccccggcg
     8161 ctacggaaga ttcaccctcc gcgtccaccc cgtagtgtaa tccaccacgt ccgctggcgg
     8221 cctacggcaa cggccggatg cggacaaaac ggttgcctgg cgttgtgggc acctgaatag
     8281 agtgcaccgg atcgccggca atgaaagtgc cgagagagga ccacgtcgca ttggtcggcg
     8341 aagaggcggc ctcagtgcgg tactcgcggc ctttaatggt cttgagcgta agctcccata
     8401 gggggttcag cgcggagttg tcgcacgaca aaatgatggg gcgggtgggt tggagaaatt
     8461 ctcgcaggtc caacgccttg gtggaaaaac ggacctcgtc gaaataggtg tactcgtcgc
     8521 ttggattggc accgtttagc gccatgccta ggcgaaccct actgttggtg tcaaagcggt
     8581 aagcgcccct cgcgcgggaa tcgagatttg tggtgtgggc gaggcggtaa tcgaaataaa
     8641 tatgcaggtt ggtggaagat ttgatgaagg caacgtggtg ccagcgggca tcgggttgat
     8701 agcggaaggt tttcgagagc caatggtaac ccgccgcggt ttgttgggaa tcgcgaaact
     8761 cagcagaaaa tcccgtggcg ttggtgtgat aggtgagcgc cacgtacgca ctggtgctat
     8821 tgaaaccgag atcactcccc cagtcgaaca gctcgtgggc ggtttcggtg ggatcgtgct
     8881 gaaaaatgaa ttccaccgtc cagtctgtca ttgtgggtgt tggcacctcg cagggtagat
     8941 aagaagaaga tgatcgccgg gaaaccactt cattggagta ttcggtttgc ccgtcgtaga
     9001 gcgggcgata agcgtaggcg ggatccgtcg gggtcagatt gatgggcctg ttgttcgtga
     9061 caccaaacgc accgacatgg tcttgatacg agggggagtc ttcatcaaag cgccaccagg
     9121 cccagacata gggcgggaag gtatccaccc ggatgttgtc cagaaataca ctgtcgccat
     9181 cgctggggga tttggtgcgg gtgaccacaa accggtcaaa gcgggtgtag ttggtccctg
     9241 cgttgtaggt tccccacgcg acaatgttgg tgacatcgta atagagccgc gtagcgccgt
     9301 tggagcggtt gagatggaag gtcacatcca caaaacgccc cgtgaccagc ggaaagctca
     9361 ccggcgagtt gtggagcttg gccacgccgt tggtgcagac aaagttgaac tcccgattcg
     9421 atagggtgtc cttcagcccg aaagaaagat tcacgttggt gtggggcaca aacagtttgg
     9481 ccgaaacgcg gatgacggga tgctccgtgc caagaagtat cgaatagatg gtcgcaaagt
     9541 tggtgtaggc ggcactactc ccgccggcag gcgtggcgat ttcaagggca tgcggcgcgg
     9601 aatacgcatt ggccgcggaa accgtcggct gcgtgggcgt cgcgatccag ccgggaacgg
     9661 tgctgatgat tccagtggcg acgttatcaa acgtctgcca gaccaattcc cccgcaactg
     9721 actgcccacc ccacaaaacc accgccaaca atacggccgt gcttcttttc atgatgacac
     9781 ctcacccact gtgttgcgta aactatagaa agaatcttcc gggcaatgca atatccgatc
     9841 cttcggaata taaagatccg ggtgcgaaac gcggaattta gtgaaagggg ggagtgttgc
     9901 ctcccatacc ggatgctttg agcatatcgc gaaccgccgg atggtgaggg tcaattgact
     9961 gggcgcggcg caaagcttct tgcgcatcgg cgtgacggtt ttgttgggtc gccaccaaat
    10021 gggccaagtg cagcccccgg acatcgccgg gttccgccaa aacatattgt tcaatccaaa
    10081 gccgcgcgtg aggccaatct gccatctgca cccgacctac cgccgcatac cacagcagtt
    10141 ccggagtgga cgggtgaagt tgcaaaccct cctccgccac cgctaaaatg gccgcggcgt
    10201 cgttggggct cccgcgatgc agataggcgc gtgccaatga aatatagact tccaccatgg
    10261 ataaacgccg ggtttggcga atggcctcca actgctggat ggcttcgtcc cattcgccca
    10321 gttgcatgtg gtcttgggcg atcgtcagac gggtaaattc agcggtttgt tcccggattt
    10381 tgaaaaaatc cattttcaac acgccaaaca acagcaaaaa taccgccacc agagctgtcg
    10441 tggagcgcca ctggcgcgcc cggataaagt catgggcgcg aacaatacca accgccgcac
    10501 ccatggcgaa aaaagggacg aggggcatgc ggtaacgccc ggtgacaaaa aacgcgatga
    10561 tggaggccga ataagtcgcc aaaaacacca aaatgagcaa aatcactgat cgtgcggtgt
    10621 tttgggcttc aaaatcggac tttttaggca aaatccacgg tgtggagacc aaaaacaggg
    10681 cgaaaatggc cgcaaaaccg agaggaatgg ctgaaagcgt gaaaaattcg ccccgtcgcg
    10741 cctgataggg agcattattg ggtatttcca cgtcaccaaa catggcaatt gccttgcgca
    10801 aggttaattt taaccattcg actggttgct cgcgaatgta atccaacgat ttagcgtacc
    10861 agtagctcga aacctccgaa ggtttcaata ggcgccccgc cgcttgttcg gcaatggcga
    10921 tggtgtcttc gcggccaccc caccaactgg cgcgggttcc tggcacaaca gcctgcattc
    10981 cattggattg cggattatta ccgatataga aatttacgcc gccttgggag gcgaccaaca
    11041 cccaatcgtc gccgactaca ccgttataga cagtggcagc caaaggaggc aaaccagcgc
    11101 caaaagcgag ccaaaatgcg aataaaatct gttttttgaa ggtcccgggc catgcccaga
    11161 gtccccaaag cgccaaaaat gggaaaaaca ccaaaatgtt gggccttgtg atggcaaaaa
    11221 ggccgaaaaa cagcccagaa attgcaccaa acatcatgtt tttccggtcc aggctccaaa
    11281 tcagcgcaag aaacccagcc aaaaccaaaa agaccagtaa aaccggaaaa aggaactggc
    11341 tgtcgaaata aatcaaaacc cagtagcacg cacacaaaag gccggcgagg ataccaacgc
    11401 gctggtcata aagacgtttc gccagcaaaa acaccaaaac gcacgaaaat gaccccaaaa
    11461 cagcctgaaa agaccgaaaa atccacaact tttcgccaaa aacgccccaa attgccgcca
    11521 aaaacatgga atagagcggc gcgcgaaaaa atgggccttc gcggagggtg tcgtaaggcg
    11581 gattccacac ccccgtggcc atgccgcgcg cccattccag attgtattcc ggatccatgt
    11641 acaccagcga gaaagccggt ttcgtactcg cttcaagcaa aaaagcggcg cgcaaaacaa
    11701 tggcaacgcc aaaaactagc aacgcaccac gccaggaact gtgcttcctc gtcatagtct
    11761 catgttgccc acaagcataa tagcaggcaa cacctttcgt cttcctctcc aaccccgctg
    11821 gcttgggttt gtccgcctgc gcgagacaaa aaatatggtt tggacttttc ggagtttgac
    11881 tttttggccg aggagttcgc ataatctttg gcgatgcggt gggtagaata ttgaaaaaaa
    11941 taagggtcta acaacaagga gacaaccatg aatgatgcaa caaacaacgt gacaccaatt
    12001 ccttcatcca at
//
LOCUS       DAOPNP010000075        11950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6172_length_11950_cov_9.224380, whole genome shotgun sequence.
ACCESSION   DAOPNP010000075 DAOPNP010000000
VERSION     DAOPNP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11950
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6172_length_11950_cov_9.224380"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PKI64_07610"
     CDS             <1..799
                     /locus_tag="PKI64_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOE00911.1"
                     /translation="VIADFEVTEAMLRYFIRKSHNRKWVRPRIIISVPSGITEVEKRA
                     VKDSAIHAGAREVYLIEEPMAAAIGVGLPVQEPAGNMIVDLGGGTTEVAIISLAGIVL
                     SRSVRVGGDEMDEAIVQYMKRVYNLMIGERTAEEIKITIGSAYPLGDEVSMEVKGRDL
                     VAGLPKTLTITSEEIREALQEPVAAVVEAIRITLERCPPELASDLVDRGIVLAGGTSQ
                     LRGLDKLLAEQTGLPVHVAEDPVSAVAEGTGVVLHELNFLRKVSTGG"
     gene            880..1767
                     /gene="mreC"
                     /locus_tag="PKI64_07615"
     CDS             880..1767
                     /gene="mreC"
                     /locus_tag="PKI64_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOE00912.1"
                     /translation="MMKKRRWIMLSALALVALVLFNLPEATSTRVKGAVRDGISPLQA
                     AWHRGLRGLRETFRYIRGLGDLALENRALSEEIVYLRGQVRMAQELARDNQLLREQLG
                     FQQRFPATLISCEVIGRDSAGWWQTVRLDKGALAGVAAERAVITTDGLIGRTVAVSDR
                     TADVLLLADPSCKVAVRVARGGVFGICTGLGNKPGEQPVCRMDYIPQTASVEEGDEVL
                     TSGLGGVFPKGLLVGHIVRVEPSESGLYRTAWIKSAADLGKLEYGFVAATETDLANAR
                     RAAETQPALDDRVAEDEWP"
     gene            1764..2261
                     /gene="mreD"
                     /locus_tag="PKI64_07620"
     CDS             1764..2261
                     /gene="mreD"
                     /locus_tag="PKI64_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOE00913.1"
                     /translation="MTLLVLLFWLLVGGTLQGVMPAWRHMGQPAFPILLGVVIYAATN
                     KKFGYFLLAALIAGVLEDSLSLVPLGSSVAAFLVAGWLALRLRTHFFADKVSHAAWFG
                     ALSAATATGTMALLLRWQGLLALPLRDALWRMAGSAVLGLVVMPVLFALMRGLERRLG
                     TWEGA"
     gene            2261..4198
                     /gene="mrdA"
                     /locus_tag="PKI64_07625"
     CDS             2261..4198
                     /gene="mrdA"
                     /locus_tag="PKI64_07625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE00914.1"
                     /translation="MPRERERERLLPEVHVWRLLLLWVALLALFGVLVHRLWSLQVAQ
                     SSEYQRRLVKQSLRSVRLPGIRGRIYDRNGERLADNQPSYCVSLYLEELRKTGSVQRT
                     VDAVMDTLYRLAETMDRPLQLTPQDVRLHLGRRTPLPLVAWRGLDEVAVARFMEHALE
                     FPGAALTVEPVRVYPQGAVAGHVLGYVGRADDTTLRDYTADHEPETYHYYLPEMAGKS
                     GIEKRLDGLLRANEGGKLEIQVDVTGFKFDEVSTRAPGMGSDITLALDLRIQRAAEQA
                     LAGERGAVVVVDPRNGDILALASMPGYNPNDFIPAVPADIWNRLLKDPARPLFNRATG
                     GEYAPGSTFKPVTLLAALESGRIKADTRYSCPGYFDLGSHRFRCWQHWGHGAIDLQAA
                     IRYSCNVYLYHAALDAGADSVQAMARAVGFGRKTDISLDFERAGLVPDAAWKRTARHD
                     NWRDGDTCNLAIGQGALLVTPLQLAMFAAALANGGTLWHPRLMARIVTASGVTNIIPA
                     TAVEDGPHWQPQNIRVVRQGMRDAVNQPDGSGKRAMLPNVVVAAKTGTAEYGVKGAGR
                     KMTWMIAFAPYDQPRYALALLVEDGISGGTTAAPRVKQLLTQVFNEVEHMNLPLPAAP
                     LRPAPDPNAPADEEPPAEEPA"
     gene            4195..5337
                     /gene="rodA"
                     /locus_tag="PKI64_07630"
     CDS             4195..5337
                     /gene="rodA"
                     /locus_tag="PKI64_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOE00915.1"
                     /translation="MKKRARNGWQLFVAQDWLAILLAAGLLAAGLTFVYSASWRGDDI
                     GIVGAWFIKQIWWVALGAGVATALAFSDYKLWLRHAWWIYLAALALLVLVLFFGKRVY
                     GAYRWLVVFGVPVQPSEFAKVALVLLLARVLASPAIRPRSFWTVLLALFLVGVPGGLI
                     LLEPDFGTAMILAPTVILVLFAAGCALRHLLLLGAVAGGGLALLLSPLARFVLSDYQQ
                     ERIATFLDPGRDPLGSGWNALQSAIAVGSGGVWGKGLLNGTQNVLGFLPRTVAPTDFI
                     FPVLAEEKGFIGASVLLLAFACLLTCYLRTAWTAKDPAGSLLATGIAALLFCHVFVNI
                     GMTIGLLPITGLPLPFVSSGGSFMVSTLAGFGLVQSVHVRRKTREV"
     gene            5339..6919
                     /locus_tag="PKI64_07635"
     CDS             5339..6919
                     /locus_tag="PKI64_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOE00916.1"
                     /translation="MWEWLKMIGLGKKIKREIIVNAESLETRVAVMEDGHLEEFAIER
                     PTEERIVGSIFKGRIQNIEDGLQAAFVDIGLKKNAFIHFWDMIPEDAARLEAEEGVAA
                     NRPRKKKTQPGEMAKQFPPGSEIIVQVTKGPIGTKGSRVTANLSIPGRYLVMMPGSKL
                     KGVSRKIEGEKERARLKKALARLPIPSSVGLIIRTSAEGVRASSLARDLRGLLDIWQK
                     IEDGIKNQSAPCCLYHEPDLVERVVRDSLTAEIDRIVIDSRAVFERIRDMTSRISRRV
                     KNRVKLYDGAAPIFEHFEVEKQLENAFRRKVWLKSGGYLIFDETEALVAIDVNTGRHK
                     GGQNQEESILQVNTEAAGEVARQLRLRNIGGLVVIDFIDMKSRKNQSAVYKTLKEALQ
                     KDKARTNVLPVSQLGLVEMTRQRMEESLRAANYADCPYCHGRGKVLSTLSMSVRIQRR
                     ILEVIKKNQHRGEIPLRIAINPMIMERLRKEDEQVLIDLEKRGHTQLTFVSDVNLHVE
                     DFRITHRQTGEVFYEEHD"
     gene            complement(6930..7952)
                     /gene="hypE"
                     /locus_tag="PKI64_07640"
     CDS             complement(6930..7952)
                     /gene="hypE"
                     /locus_tag="PKI64_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOE00917.1"
                     /translation="MNCPLPTSRYDTITLAHGGGGTLTASLLNEIFLPAFGPPAPAPH
                     DASAFAIGPQQLAITTDSYVVQPLFFPGGNIGSLAVNGTVNDLLMAGARPRALTASFI
                     LEEGLPLATLRTVVDTMQQAAQAAGVRIIAGDTKVIHRANGDGLYITTTGVGDIVTPA
                     PLAPERIAAGDQIILTGDLGRHGIAVMAQRLGLTFEPPVQSDCAALSAPLLELFAAGI
                     PLHCARDATRGGLGGILCELAAAAQLAVEINEAALPVAPAVRSACELLGFDPLFVANE
                     GCAALFVAAADVEPTLAILRRYAVTAQALVIGQVAAGRGVTARTALGTTRRLVPPSGE
                     QLPRIC"
     gene            8284..9300
                     /locus_tag="PKI64_07645"
     CDS             8284..9300
                     /locus_tag="PKI64_07645"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOE00918.1"
                     /translation="MNIEEVDGLTAAALAALGSAADLAALEALRVKYIGRNGLLPELM
                     QGLKAVPAADKPALGQALNRFKVSVTAAVAARQAALAAAAKAAKAGAFDWSLPGRWAR
                     QGRRHPIQQLIDRTSEIFRRMGFTVADGPDVETEYYNFDALNTPADHVSRDVQDTFYL
                     PGGLLLRTQTSAVQARYMESHPPPVRIVCPGRCYRRDTTDATHSANFHQIEGLYVDTR
                     VSLADLKGDIDHFARELMGPGVKSRFRTHFFPFTEPSVEVDFSCHMCGGAGCRVCKQS
                     GWIEIMGAGMVDPNVLQLAGYDPAAVMGYAFGMGIERLAMILFGIDDIRRLYENDVRY
                     LAQF"
     gene            9310..11712
                     /gene="pheT"
                     /locus_tag="PKI64_07650"
     CDS             9310..11712
                     /gene="pheT"
                     /locus_tag="PKI64_07650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOE00919.1"
                     /translation="MNIPLSWLQEYVDIPDTPEGLAERLTFSGVEVEGIRRVGGGVPG
                     VVVGEVVGIEKHPNADKLTLCRVRYGAAAETTVVCGAPNAAVGGKYPFAPLGTALPCG
                     ITIAKRKVRGVASEGMLCAEDELGLSEDHTGLLELAAHWAPGTPLAEVLGPPETVLEV
                     EITPNRPDCLSLLGLAREVAALYGTEVKWPDTNLPEVHPAVEKATSVIVEDLTDCPRY
                     TARVLHDVRVGPSPEWLRRRLEAAGIRAINNIVDITNYVMLEYGQPLHAFDQKLLAEG
                     RIVVRHPRPGEKILTLDGVERTLEPQMLVIADAQRAMAVAGVMGGEDSGINERTTDVL
                     LESASFRAGAVRATSKKLGMLSDASYRFARGVDIGLAEVASRRAAKLMVELAGAKVLM
                     GVVDVHAPLPQPWRVTCRPARLHALLGLAPEPAAIAKDFTALGLKVLTCDAERIEVEV
                     PTFRGDLTREADLIEEYARLHIRELPAVRPLATIVPGADDRPALAAARLREVLVGLGL
                     QQIMNYSFLAEGLLDLFDGGAAAQRVKLANPLTADHTVMRDALLPQMVETIGLNVSRQ
                     VTQAALFETGRVFRLGADGLPAEADHVAIGLAGAVGRAGMDQFQPVSEQDMFLWIKGV
                     WEQAAAALNVADVTLRPADRPWSEPGRGLELVAGAEVIGWLGLLKTDIAKEWRIHEPV
                     GLLEAAVAPLVKNVVAIRPLAPPPSYPSIRRDAALIVAPDVRHEQVLAVAREAAPPEL
                     ERIELFDIYTGKNLAGRKSMAYAFTYRSATGTLTDEQANAFQEKVNAALVARLPAEIR
                     AG"
     gene            11741..>11950
                     /locus_tag="PKI64_07655"
     CDS             11741..>11950
                     /locus_tag="PKI64_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015274273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE00920.1"
                     /translation="MPTYEYQCRKCGHEFEKFQSITAAPVKTCPKCRGRVARLVSGGA
                     GIIFKGTGFYQTDYKNAKSPSTSATS"
BASE COUNT         2037 a         3625 c         4218 g         2070 t
ORIGIN      
        1 cgtcatcgcg gatttcgagg tgaccgaggc gatgctgcgg tatttcatcc gcaagtcgca
       61 taaccgcaag tgggtgcgcc cccgcatcat catttcggtc cccagcggaa tcacggaagt
      121 ggaaaagcga gcggtcaagg attccgcgat ccatgcaggc gcgcgggaag tgtacttgat
      181 cgaggagcca atggcggcgg caatcggcgt gggccttccg gtccaggagc cggcgggcaa
      241 catgattgtg gacctgggag gcggcaccac cgaggtggcg atcatctccc tggccgggat
      301 cgtgctgagc cgcagtgtgc gcgtcggcgg cgacgagatg gatgaagcaa ttgtccaata
      361 catgaagcgg gtgtacaacc tgatgattgg cgagcggacg gcggaagaga tcaagatcac
      421 gattggttcc gcctatcccc ttggcgatga ggtgagcatg gaagtgaagg gccgcgacct
      481 cgtggccgga ctgcccaaga ccctcacgat tacgtccgag gaaatccgcg aggcactgca
      541 agagccggtg gccgccgttg tcgaagccat tcgcatcacg ctggaacgtt gcccaccgga
      601 gctggcctcg gaccttgtgg accgcggcat tgtgctggcg ggcggcacct cgcaactgcg
      661 gggcttggat aaattgctgg ccgagcagac gggcctgccc gtccacgtgg ccgaggaccc
      721 ggtgagcgca gtggccgaag gcacgggtgt cgtgctgcac gagctgaact tcctgcgcaa
      781 ggtctccact ggcggctaag ccggcggccc gcccgtccct catcctgaac gctttctttt
      841 cccttctgcc gggcaaacat tgccggctgg ccggaaggaa tgatgaagaa acgacgttgg
      901 atcatgttga gcgcgctggc gctggtggcg ctggtgctgt tcaatttgcc ggaggccacc
      961 tccacgcgcg tgaagggcgc ggtgcgcgat ggcatcagcc cgctgcaagc cgcctggcac
     1021 cgcggcctgc gcggcttgcg cgaaactttc cgttacatcc gcggcctcgg cgatttggcg
     1081 ctggaaaacc gcgcgctctc cgaagaaatc gtctatctgc gcggccaagt gcgcatggcg
     1141 caggaactgg cgcgcgacaa ccaactgctg cgcgagcaac tgggctttca gcagcgcttc
     1201 cccgccacgt tgatttcgtg cgaggtcatc gggcgcgata gcgccggctg gtggcagacc
     1261 gtgcgcctcg acaagggcgc gctggccggc gtggccgcgg aacgcgcggt catcaccacc
     1321 gatggcctca tcggccgcac ggtggcggtg tcggaccgca cggcggatgt gctgctgctg
     1381 gcggacccct cctgcaaggt ggcggtgcgc gtggcgcgcg gcggcgtgtt cggcatttgc
     1441 acgggtctcg gcaacaagcc gggcgagcag ccggtgtgcc gcatggatta cattccgcaa
     1501 accgcctccg tggaggaagg cgatgaagtc ctcacctccg gcctcggcgg cgtgtttccc
     1561 aagggcctgc tggtggggca catcgtgcgc gtggagccca gcgagtcggg gctgtatcgc
     1621 acggcgtgga tcaaatcggc ggcggacctc ggcaagctgg aatatggctt tgtggccgcc
     1681 acggaaaccg atttggccaa tgcgcggcgc gccgcggaaa cccagccggc gctggacgac
     1741 cgtgtggcgg aggatgagtg gccatgaccc tgctggtgct gctgttctgg ttgctggtgg
     1801 gcggcacgct gcaaggcgtg atgcccgctt ggcggcacat ggggcagccg gcgtttccca
     1861 ttttgctggg cgtggtgatc tatgccgcca ccaataaaaa gttcggctac ttcctccttg
     1921 ccgcgctgat tgcgggcgtg ctggaagatt cgttgagcct cgtgccgctg ggcagttccg
     1981 tggcggcgtt tctcgtggcc gggtggctgg cgctgcggct gcgcacccac ttttttgcgg
     2041 acaaagtcag ccacgcggcg tggttcggcg cgctcagcgc ggcgacggcc acgggaacca
     2101 tggcgctgct gctgcgctgg cagggcctgc tggcgttgcc actgcgcgac gcgctctggc
     2161 gcatggccgg ctccgcggtg ctggggctgg tggttatgcc ggtacttttt gcgttgatgc
     2221 gtggcctcga acggcggctg ggcacgtggg agggcgcctg atgccccgcg agcgcgagcg
     2281 cgagcgcttg ttgccggaag tccacgtctg gcggctgctg ctgttgtggg tggcgctgct
     2341 ggcgcttttc ggcgtgctgg tgcaccggtt gtggagtttg caggtggcgc aaagctcgga
     2401 atatcagcgc cgcctcgtga agcaaagcct gcgcagcgtg cggctgccgg gcatccgcgg
     2461 gcgcatctat gaccgcaacg gcgaacgtct cgccgacaac cagccgagct actgcgtctc
     2521 gctctatctc gaagaactgc gcaagactgg cagcgtgcag cgcaccgtgg atgcggtgat
     2581 ggacacgctg taccggctgg ccgaaaccat ggaccgcccg ctgcaactga ccccgcagga
     2641 tgtgcgcctg catttgggcc ggcgcacgcc gctgccgctg gtggcctggc gcgggttgga
     2701 cgaagtagcc gtggcgcgct tcatggaaca cgcgctcgaa ttccccggcg cggcgctgac
     2761 agtggagccg gtgcgcgtct atccacaggg cgcggtggct ggccatgtcc tgggttatgt
     2821 gggccgcgcc gacgacacca cgctgcgcga ctacaccgcc gaccatgagc cggagaccta
     2881 ccactattat ctgcccgaaa tggccggcaa gtccggcatt gaaaaacggc tcgatggcct
     2941 cctgcgcgcc aacgagggcg gcaagctgga aatccaagtg gacgtgaccg gcttcaagtt
     3001 tgacgaagtc tccacccgcg ctcccggcat gggcagcgac atcacactgg cgctggattt
     3061 gcgcatccag cgtgcggcgg aacaggcgct ggccggcgag cgcggcgccg tggtggtggt
     3121 ggacccgcgc aacggcgaca tcctcgcgct ggccagcatg cccggctaca accccaacga
     3181 tttcatcccc gccgtgcccg cggatatttg gaaccggctg ctgaaggatc ccgcccgtcc
     3241 gctcttcaac cgcgccacgg gcggcgaata tgcgccgggc agcacgttca aacccgtcac
     3301 gctgctggcc gcgctggaga gcgggcgcat caaggccgac acgcgctact cgtgccccgg
     3361 ctacttcgat ctgggctcgc accgcttccg ctgctggcag cactggggcc acggcgccat
     3421 agatttgcaa gcggccatcc gctactcctg caacgtctat ctctaccacg ccgcgctcga
     3481 cgccggcgct gactccgtgc aggccatggc gcgcgccgtg ggctttggcc gcaagacgga
     3541 catcagcctc gatttcgagc gcgccggcct cgtgcccgat gccgcgtgga agcgcaccgc
     3601 gcgccacgac aactggcgcg acggcgacac ctgcaacctc gccatcgggc agggggcgct
     3661 gctggtgacg ccattgcaac tggcgatgtt cgccgccgcg ctggccaacg gcggcacgct
     3721 gtggcatccg cgcctcatgg cgcgcatcgt caccgccagc ggcgtgacca acatcattcc
     3781 cgccaccgcc gtggaggatg gcccgcactg gcagccgcag aacatccgcg tggtgcggca
     3841 aggcatgcgc gatgccgtca accagcccga cggctccggc aagcgcgcca tgttgcccaa
     3901 tgtggtcgtg gccgccaaaa ccggcaccgc cgaatatggc gtgaagggcg cgggccgcaa
     3961 aatgacgtgg atgatcgcct ttgcccccta cgaccagcca cgctacgcac tggcgctgct
     4021 ggtggaggat ggcatcagcg gcggcaccac cgccgcgcct cgcgtgaagc aactgctcac
     4081 gcaggtgttc aacgaggtgg agcacatgaa cctgccgctg cccgccgcgc cactgcgccc
     4141 ggcgccggac cccaacgcgc ccgccgatga agaaccgccc gccgaggagc ccgcatgaaa
     4201 aagcgcgcgc gcaacggctg gcagcttttt gtggcgcagg attggctggc gatcctgctc
     4261 gccgccggct tgcttgcggc ggggttgacg tttgtttaca gcgcctcgtg gcgcggcgac
     4321 gacattggca tcgtcggcgc gtggttcatc aagcagattt ggtgggtggc gctgggcgcg
     4381 ggcgtggcca ccgcgctggc cttcagcgat tacaaactct ggctgcgcca cgcgtggtgg
     4441 atttatttgg cggcgctggc gctgctggtg ctggtgttgt tcttcggcaa aagagtttat
     4501 ggcgcctacc gctggctggt ggtgtttggc gtgccggtgc agccgtcgga gttcgccaag
     4561 gtggcgctgg tgctgttgtt ggcgcgggtg ctggccagcc cggccatccg cccgcgttcg
     4621 ttctggacgg tgctgttggc gttgtttttg gtcggcgtgc ccggcggcct gattctgctg
     4681 gaacccgatt tcggcaccgc gatgattttg gcccccaccg tcattttggt gctgtttgcg
     4741 gcgggctgcg cgctgcgcca tttgctgctg ctgggcgcgg tggcgggggg ggggctggcg
     4801 ctgctgcttt cgccgctggc gcgctttgtg ctgagcgatt atcagcagga gcgcatcgcc
     4861 acgtttctcg accccgggcg cgatccgctc ggctccggct ggaatgcgct gcagtcggcc
     4921 atcgcggtcg gttcgggcgg ggtatggggc aaaggactgt tgaatgggac gcaaaacgtg
     4981 ctgggctttt tgccgcgcac ggttgcgccc acggacttca ttttcccggt gctggccgag
     5041 gaaaaggggt ttattggcgc cagcgtgctg ctgttggctt ttgcctgcct gctgacgtgc
     5101 tatctgcgca cggcatggac ggccaaagat cccgccggca gcttgttggc gacgggtatc
     5161 gccgctttgt tgttctgcca tgttttcgtg aacatcggga tgaccatcgg cctgctgccg
     5221 attacggggc taccgcttcc gtttgtcagc agcggcgggt cgttcatggt gagcacgctg
     5281 gcgggcttcg ggctggtgca aagtgttcat gtgcgtcgga aaacgcgcga ggtgtaatat
     5341 gtgggaatgg ttgaaaatga tcggactagg caaaaaaatc aaacgggaaa tcatcgtcaa
     5401 cgcggagagt ttggaaactc gcgtggcggt catggaggat ggccacctcg aagagtttgc
     5461 catcgagcgc cccaccgagg agcgcatcgt gggcagcatc ttcaaggggc gcatccagaa
     5521 catcgaggac ggcctgcaag ccgccttcgt ggacatcggc ctcaagaaaa acgcgttcat
     5581 tcacttctgg gacatgattc ccgaagacgc cgcgcggctg gaggctgagg aaggcgtggc
     5641 cgccaaccgg ccgcgcaaaa agaaaactca gcccggcgag atggccaagc aatttccgcc
     5701 cggcagcgag atcattgtgc aagtgaccaa ggggcccatc ggcaccaagg gctcgcgcgt
     5761 gacggccaac ctgagcatcc ccggccgcta cctcgtgatg atgccgggca gcaagctcaa
     5821 gggtgtctcg cgcaagatcg agggggagaa ggagcgcgcg cgcctcaaga aggcgctggc
     5881 ccgcctgccc attccctcca gcgtggggct catcatccgc acctccgctg agggcgtgcg
     5941 cgccagcagt ctcgcccggg atttgcgcgg cctgctcgac atctggcaga aaatcgaaga
     6001 cggcatcaaa aaccagtccg cgccctgctg cctctatcac gagcccgacc tcgtcgagcg
     6061 cgtggtgcgc gattcactga ccgccgagat tgaccgcatt gtcattgatt cgcgcgcggt
     6121 gtttgagcgc atccgcgaca tgaccagccg catctcgcgc cgcgtgaaaa accgcgtgaa
     6181 gctctacgac ggcgccgcgc ccatctttga gcattttgag gtggagaagc agttggaaaa
     6241 cgccttccgc cgcaaggtat ggctcaagtc cggcggctat ttgatcttcg acgaaaccga
     6301 agcgctggtg gccattgacg tgaataccgg acggcacaag ggcggccaga accaggagga
     6361 aagcatcctg caagtcaaca ccgaggccgc cggcgaggtg gcgcgccagc tccggctacg
     6421 caacatcggt gggctggtgg tgattgactt cattgacatg aagtcgcgca agaatcaatc
     6481 ggcggtgtac aaaaccctca aggaggcgct gcagaaggac aaggcgcgca ccaacgtgct
     6541 gccggtgtcg caactggggc tggtggaaat gacgcgccag cgcatggagg aaagcctgcg
     6601 cgccgccaac tacgccgact gtccctactg ccacgggcgc ggcaaagtgc tctccacgct
     6661 gagcatgagc gtgcgcatcc agcggcgcat tttggaggtc atcaagaaga accagcatcg
     6721 tggcgaaatc ccgctgcgaa ttgccatcaa cccgatgatc atggaacgcc tgcgcaagga
     6781 agatgagcag gtgctcattg acctcgaaaa acgcgggcac acgcaactga cgtttgtcag
     6841 cgacgtcaac ctgcacgtgg aagatttccg catcacccac cggcagacgg gcgaagtctt
     6901 ctacgaagag cacgactagg cgcggcgcgt tagcaaatcc gcgggagctg ttcgccggag
     6961 ggcggcacga ggcggcgcgt ggtgcccagc gcggtgcgcg cggtcacgcc gcgccccgcc
     7021 gccacttgac cgatcaccag cgcctgcgca gtgacggcgt accggcgcag gatggccagt
     7081 gttggttcaa catccgccgc cgccacgaac agcgccgcgc agccttcgtt ggctacaaag
     7141 agcggatcaa accccagcag ctcgcacgcg ctgcgcacgg caggcgcgac gggcagggcg
     7201 gcttcgttga tctccaccgc cagttgcgcg gcggcggcca gctcgcacaa aatgccgccg
     7261 aggccgccgc gggtggcgtc gcgcgcgcag tggaggggaa tgccggccgc gaatagttcc
     7321 agcagcggcg cgctcaacgc ggcgcaatcg ctctgcacgg gcggctcgaa cgtcaggcca
     7381 aggcgctggg ccatgacggc gatgccgtgg cggccgagat cgccggtcaa aataatctgg
     7441 tcgccggcgg cgatgcgctc cggggcgagg ggggcggggg tgacgatgtc gcccacgccg
     7501 gtggtggtga tgtagaggcc atcaccgttg gcgcggtgga tcaccttggt gtcgccggcg
     7561 atgatgcgca cgccggcggc ttgcgcggcc tgttgcatgg tgtccaccac ggtgcgcagc
     7621 gtggccagcg gcaagccctc ttccagaata aagctggcgg tcagcgcgcg aggtcgcgcg
     7681 ccggccatga gcaggtcgtt cactgtgcca ttgacggcga gagagccgat gttgccgccg
     7741 gggaaaaaga gcggctggac cacgtaggaa tcggtggtga tggccagttg ttgcgggccg
     7801 atggcgaaag cgctggcatc gtggggggcg ggggcggggg ggccgaaggc gggtaggaaa
     7861 atctcgttga gcaggctggc ggtcagcgtg ccgccaccgc cgtgggcgag ggtaatcgtg
     7921 tcgtagcgcg aggtgggcag ggggcaattc atggcgtcaa caactacggt ttcgccccgc
     7981 ggaagaaaag ccaaaagcgg cgccggcgcg tgttttcggc ttttcatttt tccacggtgt
     8041 ggaaaaatgg ccggacattt ttccacggta tggaaaaaat ttcatcccgc gggcgcggga
     8101 ttttccacag tgtggaaaaa ccggccggat ttttccacgc aatggaaaaa ctttgcgcga
     8161 tttttccaca caatggaaaa actttggtgc ggtttttcca cacaatggaa aaactttgcg
     8221 gattttttcc acggtgtgga aagatttttt ccacagtgtg gaaaagtgga gttgaggagc
     8281 aagatgaaca tcgaggaagt ggacggattg acggcggcgg cgttggcggc gctgggcagc
     8341 gcggcggatt tggcggcgct ggaggcgttg cgggtgaagt atatcggccg caacgggctg
     8401 ttgccggagc tgatgcaggg gctcaaggcc gtgccggcgg cggacaagcc ggcgctgggg
     8461 caggcgctca accgcttcaa ggtgtcggtg acggccgcag tggcggcgcg gcaggcggcg
     8521 ctggcggcgg cggccaaggc ggccaaggcg ggggcgttcg attggtcgct gccggggcgc
     8581 tgggcgcggc agggacggcg gcaccccatc cagcaactga ttgaccgcac cagcgagatt
     8641 ttccggcgga tgggtttcac ggtggcggac gggccggacg tggagacgga gtattacaat
     8701 ttcgacgcgc tcaacacgcc ggcggaccat gtctcgcgcg atgtgcagga cacgttttat
     8761 ttgccgggcg ggctgctgct gcgcacgcag accagcgcgg tgcaggcgcg ctacatggaa
     8821 agccatccgc cgccggtgcg cattgtgtgt ccggggcgct gctaccggcg cgatacgacg
     8881 gatgccacgc atagcgccaa cttccaccag atcgaggggc tttatgtgga cacgcgcgtg
     8941 agcctcgccg atttgaaggg cgatattgac cacttcgcgc gcgagctgat ggggccgggc
     9001 gtgaagtcgc gcttccgcac gcactttttc ccgttcaccg agccgagcgt ggaagtggat
     9061 ttttcctgcc acatgtgcgg cggggcgggc tgccgggtct gcaagcagag cggctggatc
     9121 gaaatcatgg gcgcgggcat ggtggacccc aatgtgctgc aactggccgg ttatgacccc
     9181 gcggcggtca tgggctacgc cttcggcatg ggcatcgaac ggctggcgat gattctcttc
     9241 ggcattgatg acatccgccg gctctacgaa aacgatgtgc gctatctggc ccaattctga
     9301 ggactcgcca tgaatattcc gttgagctgg ttgcaagaat atgtggacat tccggatacg
     9361 ccggaagggc tcgccgagcg tctgacgttt tccggcgtgg aggtggaagg catccgccgc
     9421 gtgggcggcg gcgtgccggg cgtggtggtg ggcgaagtgg tgggcatcga gaagcatccg
     9481 aatgccgaca agctgacgct ctgccgcgtg cgctacggcg cggcggcgga aaccaccgtg
     9541 gtgtgcggcg cgccgaatgc ggcggtgggc ggcaagtatc cgttcgcgcc gctgggcacc
     9601 gcgctgccgt gcggcatcac catcgccaag cgcaaggtgc gcggcgtggc gagcgagggc
     9661 atgttgtgcg ccgaggatga actggggctt tccgaggacc acacggggct gctggaattg
     9721 gccgcgcatt gggcgccggg cacgccgctg gccgaagtgc tggggccgcc ggaaaccgtg
     9781 ttggaagtgg aaatcacgcc gaatcgtccg gactgcctga gtctgttggg cctcgcgcgc
     9841 gaagtggccg cgctctatgg cacggaagtc aaatggcccg acaccaacct gccggaggtg
     9901 catccggccg tcgaaaaggc cacgagcgtg atcgtggagg atttgaccga ttgcccgcgc
     9961 tataccgcgc gtgtgctgca tgatgtgcgc gtggggccca gccccgagtg gctccggcgc
    10021 cgcttggagg ccgccggcat ccgcgccatc aataacattg tggacatcac caactacgtg
    10081 atgctggagt atggtcagcc gctgcacgcg ttcgaccaga agctgctggc cgaagggcgc
    10141 attgtggtgc gccacccgcg ccccggcgaa aagattctca cgctggatgg cgtggagcgt
    10201 acgctggaac cgcaaatgct ggtgattgcc gatgcccaaa gggccatggc tgtggccggc
    10261 gtgatgggcg gcgaggatag cggcatcaac gagcgcacga cggacgtgct gctcgaaagc
    10321 gccagcttcc gcgcgggcgc ggtgcgcgcc accagcaaga agctgggcat gctctcggat
    10381 gcgtcgtacc ggttcgcgcg cggggtggat attggactgg cggaagtggc cagtcgccgc
    10441 gcggcaaaat tgatggtgga gctggccggc gccaaggtgc tgatgggcgt ggtggatgtc
    10501 cacgcgccgc tgcctcagcc gtggcgggtg acgtgtcgcc cggcgcggct gcacgcactg
    10561 ctggggctcg cacccgagcc ggcagccatc gccaaggatt tcaccgcact gggcctcaag
    10621 gtgctgacgt gcgatgccga gcgcatcgag gtggaagtgc ccacgttccg cggcgacctg
    10681 acgcgcgaag ccgatttgat cgaggaatac gcgcggctgc acatccggga gctgccggcg
    10741 gtgcgcccat tggcgaccat cgtgcccggc gccgatgacc gcccggcgct ggccgcggcc
    10801 cgtctgcgtg aggtgctggt gggcctcggc ttgcagcaaa tcatgaatta cagctttttg
    10861 gcggagggct tgctggacct gtttgacggc ggcgcggccg cgcagcgcgt caaactggcg
    10921 aatccgctga cggcagacca caccgtgatg cgcgacgcgc tgttgccgca aatggtggag
    10981 accatcgggt tgaatgtgtc gcgccaagtg acgcaagccg cgctgttcga gacggggcgc
    11041 gtgttccgcc tcggcgcgga tggcttgccg gcggaggccg accatgtggc cattgggctg
    11101 gccggcgcgg tggggcgcgc cgggatggac cagttccagc cggtgagcga gcaggacatg
    11161 tttttgtgga tcaagggtgt gtgggagcag gcagccgctg cgctgaacgt ggcggacgtg
    11221 acgctgcgcc ccgccgaccg cccctggagc gagcccggcc gcggcctgga attggtggct
    11281 ggcgcggagg tgatcggctg gctcggcttg ctcaagaccg acatcgccaa ggaatggcgc
    11341 atccacgagc cggtggggct gctggaagcg gccgtggcgc cgttggtgaa gaatgtggtg
    11401 gccatacgcc cgctggcgcc gccgccgagc tacccgagca tccggcgcga cgccgcgctg
    11461 attgtcgcgc ccgacgtgcg ccacgagcaa gtgctggcgg tggcccgcga ggccgcaccg
    11521 ccggagttgg agcgcatcga gctgttcgac atctacaccg gcaagaatct cgccggacgc
    11581 aagagcatgg cctacgcgtt cacctatcgt tccgccaccg gcacgctgac ggatgaacag
    11641 gccaacgcct ttcaggaaaa agtgaacgcg gcgctggtgg cccggctgcc ggcagaaatc
    11701 cgcgcgggct aggccgcgcc agaccccaga ggtgatctcc atgccaactt atgaatacca
    11761 atgccgcaag tgcggacacg agtttgagaa gtttcagtcc atcaccgccg caccggtgaa
    11821 gacctgcccc aagtgccgcg ggcgggtggc gcggctggtt tcgggtggcg cgggtatcat
    11881 cttcaagggc acgggatttt atcagacgga ttacaagaac gcgaaatctc ccagcactag
    11941 cgccacgtcc
//
LOCUS       DAOPNP010000076        11832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6245_length_11832_cov_9.426679, whole genome shotgun sequence.
ACCESSION   DAOPNP010000076 DAOPNP010000000
VERSION     DAOPNP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11832
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_11832_cov_9.426679"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKI64_07660"
     CDS             <1..183
                     /locus_tag="PKI64_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00921.1"
                     /translation="GHAATLYAVWLARATVLSDASARRYFTTENTEKHRDEKKDRAEP
                     SDRSNPVCGLPLNPEP"
     gene            complement(200..2317)
                     /locus_tag="PKI64_07665"
     CDS             complement(200..2317)
                     /locus_tag="PKI64_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00922.1"
                     /translation="MELLAKKPAWFWCVAVAVAVFLVYAPTLGHDFSLMDDAYFVTEN
                     PVVQGGLTLAGVKAAFTGVYASYWAPLLWLSFMVDQTVSGGAPWSFHLTNVVLFALSA
                     GLLFLLAVSWTGGRLGVSLAAALLWALHPIRVESVAWITERKDVLSGIFFLLGLWCYT
                     RARSRVASSVRRGVLLGFAWFSMLLGGMAKQVIFVMPAAFVLLDVWPLQRTSWDRLGR
                     DLWRLIAEKWAFWLLTAIFLIVAYGTQSEGGAIMTIPLTLRLQMLPVHYLFYLEKTFW
                     PTTLAPLQADLPPEIWRVTVGVVILIGLTGVLWELRKKSPWALWGWLWFVGLIFPLSG
                     LIWAGSERVALRFLYLPHIGLSLALLMGLNAVAQRRPALHALVRGGVAVVLLFAAGAT
                     LRTQNYWRDPNAFGLWIYECHPEQGGACAMGGDTYLAGGAWDLALDAYAKGAAMPDRH
                     CFIRQCSVWNQLGYPERTAQAWPEFERALGKPLAEFAPWERPTERELFWRVRGQALLA
                     LRDYPAAIAALREAVRWEPDPGSFTVAEYLRACHEADRPEEAGAAAVAMAKATGIDVR
                     EWKDLLPCYVEFWKTGARGYAYRFFEAYAERFPDDSMPLSFLAWLLATAEPDGRDHAR
                     QEEWPQTALHWAEQALDQTQNPPANMWLTLAAARANAGHFSEAVPAAEKARELAKDNG
                     DNALLEVIEKQIMNYRLGLAWRE"
     assembly_gap    2501..2600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2833..3813)
                     /locus_tag="PKI64_07670"
     CDS             complement(2833..3813)
                     /locus_tag="PKI64_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00923.1"
                     /translation="MPSSPKSKIAFAHFQQAFAVNRMPHAVLVNGAPRGAGGAFAEQL
                     LSLPFQETDPARLRAHVDIRWIEPESKSRQITIDDQIRPLLDFIALTSYEGGWKAAVI
                     LFADRLNINAQNALLKTLEEPPPRSLLILVTDIPAALLPTIRSRTQYVDVTTATRDTD
                     APWLETVLDLLRNPPARQAIDMIAWTDRLTAPLRELKELAEADETAAAEQTAQAQDGE
                     VALTEATKSMVEGRVATRVKEMREDVLRTILLWQRDVLAAACHGENTPANFPDEAAAI
                     AAQAKDLSFAEAAARVDAVEEVRSLMEHNIRDEAALLRLARALSTPPINQ"
     gene            complement(3791..4441)
                     /gene="tmk"
                     /locus_tag="PKI64_07675"
     CDS             complement(3791..4441)
                     /gene="tmk"
                     /locus_tag="PKI64_07675"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOE00924.1"
                     /translation="MERGKFITFEGPEGAGKSTQAAMLIAKLEAHGIEVIYTREPGGT
                     KLGEAIRGILQYNAANEDPCPESEVLLFEASRAQLVRHVIAPALARGAWVLCDRFADS
                     TTAYQGFGRGFPVDLMETINRFAIGSAVPDMTILLDVNVSLGMQRCAKRQVGQKIKYD
                     RIESEALAFHEKVRQGYLELARRYPERFRKVDAMRLPEPIAEDIWKLVHDAFLAKK"
     gene            complement(4496..5896)
                     /gene="cysS"
                     /locus_tag="PKI64_07680"
     CDS             complement(4496..5896)
                     /gene="cysS"
                     /locus_tag="PKI64_07680"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE00925.1"
                     /translation="MSVFRVHNTLTRRLEEFQPLEPGHARLYTCGPTVYNYAHIGNFR
                     AYVFEDLLRRWLKFKGFRVTQVMNLTDVDDKTIRDSQAAGLSLAEFTARYKQAFFEDI
                     RVLNIEAAEHYPAATDHIPEMIALIQVLQDKGYAYRADDGSVYFSIDRWPQYGKLAHL
                     DRSGLRAGVRVNADEYDKENVADFALWKAWDEKDGDVAWDSPWGRGRPGWHIECSAMS
                     QRYLGPSFDIHTGGVDNIFPHHEDEIAQSEAATGQPFATYWLHCAHLLVDGQKMSKSR
                     GNFHTLRDVLQRGYTGREVRYELLATHYRASLNFTFASLDAARTALRRVDEFTARLTD
                     AAGSAASAELPTWARLAATRFEAAMDEDLNISAALAALFDAIREGNAALDARALAPSA
                     AAAVQALLARWDTALGVLAKPATAIPADVQALLDRRQAARTAKDWAQSDALRGEIANL
                     GWNVKDTPKGQVATPR"
     gene            complement(5893..8445)
                     /locus_tag="PKI64_07685"
     CDS             complement(5893..8445)
                     /locus_tag="PKI64_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOE00926.1"
                     /translation="MNNFTPRAQRVLQLAKQEADRFNHGYIGTEHLLLGLIALGEGVA
                     VTVLRRLGLDLDTVRMEVEKAVGVGSDMKIVGNVPYTPRVKKVLALAGTEARAMNMDY
                     VGTEHILLGLLREAEGVAARVLTNLHVDIEKTRDAVLKELDPNYEAPPADEETPGDAP
                     GPELGPGPDKARGPKEQKTPALSTFGRDLTALAQKGALDPVIGRADEIERVMRILCRR
                     TKNNPALLGEAGVGKTAIAEGLAQAIVNGEVPELLLGKRVISLDLALMVAGTKYRGQF
                     EERIKAVMDEIRRVKNVILFVDELHSLVGAGSAEGAMDAANILKPALSRGELQVIGAT
                     TLNEYRKFIEKDAALERRFQSVLVREPSIEESIEILKGLRARYEAHHHARFGDDAIEA
                     AVRLSARYLQDRFLPDKAIDVIDEAGARARIGAMTRPVHVKEKEREIANLLEQRKVAI
                     ENEQFEEAAKLRDAERDMRAALEKELADWRRDHTERIVDVNEEDVRLIVSKWTGVPLE
                     KMEQKETAKLLEMEAALAKTVIGQQDAISSICKALRRSRADLKDPRRPIGSFIFLGPT
                     GVGKTLLAKSLAEFMFNSPDALIQIDMSEYMEKFNASRLVGSPPGYVGYEEGGQLTER
                     VRRRPYSVVLFDEIEKAHPDVMHMMLQILEEGRLTDGLGRAVDFRNTIIIMTSNIGSE
                     AARKGAGLGFAEHTAAADYAALREQMMEAAKQNFRPELLNRVEEIIVFRQLQREDVRT
                     ILEIEVGKVAARLKAREIKLSVLASAYDFLLNKGFDRAYGARQLRRAVERWLENPLAE
                     EILRGNFSQGEVVEVEAGDDRLDFHLPTGEPPPETEAAAPVRRRKKKDADSP"
     gene            complement(8438..9484)
                     /locus_tag="PKI64_07690"
     CDS             complement(8438..9484)
                     /locus_tag="PKI64_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOE00927.1"
                     /translation="MSESANYADAHAVVLSSRVRLARNLRGLPFPDRAPSAVRAEVCQ
                     RVMDAARERLPLQEALAGAPTLRRQLLFEGHLISRHLLTERPESAICMDAAHSCTVMI
                     NEEDHLRIQVLQPGLALEKAWQVANDLDDFLEQKLDYAFSSTLGYLTSCPTNVGTGMR
                     ASAMMHLPALVLQNEMEPIANGLAKIGLTVRGRWGEGTAALGHMFQVSNQRTLGLTEE
                     RIVEDLGEIVRELEGHEQNARARLKRERRLFVEDLAARAKGILTAARLMTAQEALERL
                     SELRLGILLGLVRGLDVQLVDELWLAVQPAHLQAAAGRAMNSAERDKARARMLRTMLK
                     GRTPKRAQKENPHE"
     gene            complement(9481..9981)
                     /locus_tag="PKI64_07695"
     CDS             complement(9481..9981)
                     /locus_tag="PKI64_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOE00928.1"
                     /translation="MKEAQFQFTKTEDGQVVKLSLCAECAAALKMPLADKTVDLSGMM
                     AELKNQLDQLQETLAADTPPAGQHACPVCGMKRTDVLKKGRLGCDRCYEVFGAEMVPV
                     VVSLQRGDQHVGKVPKRISSGLRLSVETGRLRRELDKAIAAEKYEEAALLRDQLRALS
                     AGKEQP"
     gene            10100..10291
                     /locus_tag="PKI64_07700"
     CDS             10100..10291
                     /locus_tag="PKI64_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00929.1"
                     /translation="MTWKDTMGALVSPLKACFDLTLAERRFLLGVLLLFALGLGARWL
                     HLRSDRSTPYTPPAEQRHP"
     gene            10288..10704
                     /locus_tag="PKI64_07705"
     CDS             10288..10704
                     /locus_tag="PKI64_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOE00930.1"
                     /translation="MSNCIFCKIVAGTIPATKLYEDDDVLAFMDIGPIVKGHVLVIPK
                     THTETIGQTPPETLAKVIAVVRRMARAQQAGLGADGINIHQSNGAAAGQVVPHIHFHV
                     IPRFKHDGHHWNWTAQSYADPSEVAALADRIKAALD"
     gene            10709..11131
                     /locus_tag="PKI64_07710"
     CDS             10709..11131
                     /locus_tag="PKI64_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00931.1"
                     /translation="MKSPTFAEIIARICARDRRYAADAFVFLSEGLQFTVKAIQEKEK
                     NRRHVTGQELALGLRDYALEQFGPLAMTVLARWGVQTTRDFGEMVFLLIGAGLFGKTE
                     EDKIEDFDDVYNFATAFRTPFLPKLTAQRQPRPRPTTA"
BASE COUNT         2215 a         3576 c         3691 g         2250 t
ORIGIN      
        1 ggtcatgccg ccacactcta tgctgtgtgg ttggcacggg caactgtttt gagcgatgct
       61 tcagccagaa gatatttcac cacagagaac accgagaaac acagagatga aaaaaaagac
      121 agggcagagc cgtcggatcg atcaaatcct gtctgcggcc tccccctaaa ccccgaaccc
      181 tgaacccttc cctcttcccc tactcccgcc aagcgaggcc gaggcgatag ttcatgatct
      241 gcttttcgat gacctccagc agcgcgttat ccccattgtc cttggcgagc tcgcgcgcct
      301 tttctgcggc cggcacagcc tcggaaaaat ggccggcatt ggcccgggca gcggccaatg
      361 tgagccacat gttggccggc gggttttgag tttgatccaa ggcctgctcc gcccaatgca
      421 gcgccgtttg cggccactcc tcttgacgcg catgatcgcg gccatcgggc tccgcggtgg
      481 ccagcagcca ggctagaaag cttagcggca tggaatcgtc ggggaagcgt tcggcatagg
      541 cctcaaaaaa gcggtaggcg tagcctcgcg caccagtttt ccaaaactca acatagcacg
      601 ggaggagatc tttccattcg cggacatcaa tgccagtggc cttcgccatg gcgacagcgg
      661 cggcaccggc ttcctccggg cggtcggcct cgtgacaggc gcgcaggtac tccgcgacgg
      721 taaaagaacc gggatcaggc tcccagcgca cggcctcccg aagcgcggca atggcggcgg
      781 ggtaatcgcg cagggcaagc agggcttgtc cccgcacccg ccaaaacagc tcgcgttcgg
      841 ttggccgttc ccaaggcgca aactccgcca gcggtttgcc caatgcccgc tcaaactccg
      901 gccacgcctg cgcggtgcgt tccggataac ccaactggtt ccatacggaa cattggcgga
      961 taaagcagtg cctgtccggc atggcagcgc ccttggcata ggcatcaagc gccaagtccc
     1021 atgcgccacc ggccagatag gtgtcgccgc ccatcgcgca ggcgccgccc tgttcagggt
     1081 ggcattcgta aatccataag ccaaaggcat tgggatcacg ccaataattc tgggtacgca
     1141 gcgtggcgcc ggcggcaaaa agcaaaacca cagccacacc gccgcgaacc aaggcatgca
     1201 aggccggccg ccgctgcgcc actgcgttca gccccatcag caacgcgagg ctcaagccaa
     1261 tatgcggcaa ataaagaaac ctcagggcca cccgctccga accagcccag atgagtccgc
     1321 tcaacgggaa tatcaacccc acaaaccaaa gccaccccca gagagcccaa ggggattttt
     1381 tgcgcagctc ccacagcacc ccagttaggc cgatgaggat gacaactccg accgtgacgc
     1441 gccatatttc cggcggcagg tccgcttgca gcggtgccaa ggttgtgggc cagaaggttt
     1501 tttcaaggta gaaaagataa tgcactggca acatttgcaa gcgcagggtc aggggaatgg
     1561 tcataatggc ccctccctcc gattgcgtgc cgtaggccac aatcaaaaaa attgcagtta
     1621 gcagccaaaa ggcccacttt tccgcgatca accgccagag gtctcgcccg aggcgatccc
     1681 acgatgtgcg ctgcaatggc cagacatcca gcagcacgaa tgccgccggc atcacaaaga
     1741 tcacttgttt ggccatcccc cccagcaaca tgctgaacca ggcaaatccg agcaggacac
     1801 cccgacgcac ggatgatgcg acccgcgaac gggcccgcgt atagcaccac agtcccagca
     1861 gaaaaaatat gccgctcagc acgtccttgc gttcggtgat ccatgccacg ctttcgaccc
     1921 ggatcgggtg caatgcccaa agcaaggcag cagccaatga cacccccaac cgcccccccg
     1981 tccagctcac ggccaaaaga aacaacaacc cggcgctcag tgcaaacaac accacgttcg
     2041 ttaggtgaaa gctccatggc gcgccaccgg aaacggtctg atccaccatg aaactcaacc
     2101 agagcaatgg cgcccaataa ctggcataga cccccgtaaa agccgcttta acccccgcca
     2161 gagtcaaccc accctgaaca actggatttt ccgtcacgaa ataggcgtca tccatcaggg
     2221 aaaagtcgtg gcccaacgtc ggggcgtaga cgaggaatac tgccactgca actgcgacac
     2281 accagaacca cgccggtttt tttgccaata gttccataac cggcatcgta cggcatacgc
     2341 ggctgcgcgc caagcattcc tcacccgtgt tacaggcggg gtcaccgccc ccgcctacag
     2401 tcaaagcaat tccgccccct gtagccggcc ccgctgaggt cggggctgtt ccagatccaa
     2461 aaatccgcgc aacagcgggg tcaccgcccc cgcctacagt nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn atccgcgcaa cagcggggtc accgcccccg cctacagttg
     2641 gagccacgct gttctgatcc aagcggggtc agcgagccag ccttcaggta ctaggagcgg
     2701 gagggggaaa aggcattttg cgcaaagggc cgatgcacgc aaagatgcgt gggagatata
     2761 taattataac cgattaatag ttagattact attttaacta ataacggtca ttgagtgttt
     2821 atgatttggt tattattgat ttatgggtgg ggtggagagg gcgcgggcga ggcgcaggag
     2881 ggcggcttcg tcgcgaatgt tatgctccat gaggctgcgg acttcctcga cggcatccac
     2941 gcgggcggcg gcttcggcaa aggagaggtc cttggcttgg gcggcgatgg cggcggcctc
     3001 gtccgggaag ttggcggggg tgttttcgcc gtggcaggcg gcggcgagga catcgcgctg
     3061 ccacaggaga atggtccgca ggacatcctc gcgcatttcc ttgacgcggg tggccacgcg
     3121 gccttcgacc atgcttttgg tggcttcggt aagggccact tccccatcct gggcttgggc
     3181 agtttgttcg gcggcagcgg tttcgtcggc ttcggccagt tcttttagtt cacgcagcgg
     3241 ggcggtgagg cggtcggtcc aagcgatcat gtctatcgcc tgccgggccg gggggttacg
     3301 caataaatcg aggacagttt cgagccaggg ggcgtcggta tcccgggtgg cggtggtgac
     3361 atccacatac tgggtgcggg agcggatggt gggcaaaagc gcggcgggaa tatcggtgac
     3421 gaggatgagc aggctgcggg gcggcggctc ttccagcgtt ttgagaaggg cgttttgggc
     3481 gttgatgttg agccggtcgg cgaacaagat cacggcggct ttccacccgc cttcgtagga
     3541 ggtcaaagcg atgaaatcca agagcggccg gatttggtca tcaatggtga tttgccggct
     3601 tttgctttcg ggctcgatcc accggatatc cacgtgggca cgcaggcggg cggggtcggt
     3661 ttcctgaaac gggaggctca gcaattgttc agcgaaggcg ccgccggccc cgcgcggggc
     3721 gccgttgacc agcacggcgt ggggcatccg gttgactgcg aacgcttgct gaaaatgagc
     3781 aaacgctatt ttactttttg gcgaggaagg catcgtggac cagcttccaa atatcctcgg
     3841 caatgggttc gggcaggcgc atggcatcca ccttgcggaa ccgctccgga tagcggcggg
     3901 ccagttccaa atagccctgc cgcacctttt cgtggaaagc aagggcttcg ctttcgatgc
     3961 ggtcgtactt gattttctgg ccgacctgcc gtttggcgca gcgttgcatg ccgaggctga
     4021 cgttcacatc cagcaggatg gtcatgtcgg gcacggcaga accgatggca aagcggttga
     4081 tggtttccat gaggtccacc gggaaaccgc ggccaaagcc ttgataggcg gtggtggagt
     4141 cggcgaagcg gtcgcagagc acccacgcgc cccgcgccag tgcaggggca atgacgtgcc
     4201 ggacaagctg ggcgcggctg gcctcgaaca ggaggacttc gctttcgggg cacgggtctt
     4261 cattggcggc gttgtattgc aggatgccac ggatagcttc gccgagcttg gtaccaccgg
     4321 gttcgcgggt atagatgacc tcaatgccgt gggcttccag cttggcaata agcatggcgg
     4381 cttgcgtgct tttgcccgcg ccctcggggc cctcgaaggt gatgaatttg cctcgttcca
     4441 tggggtatcc tttattgacg ggttcggatg gtggctggcg cgggcatggt gtcggtcagc
     4501 gcggggtggc gacttgccct ttgggggtgt ctttaacgtt ccagccgagg ttggcgattt
     4561 cgccgcgcag ggcatcggac tgcgcccaat ccttggcggt gcgagcggct tgccggcgat
     4621 ccagcagggc ttgcacatcg gccgggatgg cggtggcggg tttggccagc acgccgaggg
     4681 cggtatccca acgggccaac agcgcctgca cggcagcggc ggcagacggg gccagagcac
     4741 gggcatcgag cgcggcattg ccttcgcgga tagcatcgaa gagcgcggcc agcgccgcgg
     4801 aaatattcag gtcctcatcc atggcggcct cgaagcgcgt ggcggcgagg cgcgcccatg
     4861 tgggcagttc cgccgaggca gcggatccgg cggcatcggt gaggcgtgcg gtgaactcat
     4921 ccacgcggcg caacgcggtg cgggcggcat ccagcgaggc gaaagtgaaa ttgagcgagg
     4981 cgcgataatg ggtggccagc agctcgtagc gcacttcgcg gccggtgtag ccgcgttgca
     5041 ggacatcgcg cagggtgtgg aaattgccgc gcgatttgga cattttttgg ccatccacca
     5101 gcagatgcgc gcagtggagc caataggtgg cgaagggctg gccggtggcg gcctcactct
     5161 gcgcaatttc gtcctcgtgg tgtgggaaaa tgttatccac gccaccggta tggatgtcaa
     5221 agctagggcc gagatagcgc tggctcatgg cggagcattc gatgtgccag ccggggcggc
     5281 cgcggcccca gggggaatcc caggccacgt cgccatcttt ttcatcccac gctttccaga
     5341 gagcaaagtc ggcgacgttt tccttgtcgt attcgtcggc attcacgcgc acgccggcgc
     5401 gcaagccgct gcggtcgagg tgcgccagct tgccgtactg cggccagcga tcaatgctga
     5461 aatagaccga gccgtcatcg gcgcggtagg catagccttt gtcttgcagc acctgaatca
     5521 aggcgatcat ttcggggatg tggtcggtgg cggcgggata atgctcggcg gcctcgatgt
     5581 taagcacgcg gatgtcttcg aaaaacgcct gtttatagcg ggcggtgaac tccgcgaggc
     5641 tgaggccggc ggcttgggag tcgcggatgg ttttgtcgtc cacgtcggtg agattcatca
     5701 cctgcgtgac gcggaagccc ttgaacttga gccagcggcg gaggaggtcc tcgaagacgt
     5761 aggcgcggaa gttgccgata tgggcatagt tatagacggt gggcccgcag gtatagaggc
     5821 gggcgtggcc gggttccagc ggctggaact cctccagccg gcgcgtgagg gtgttgtgga
     5881 cgcgaaagac gctcatggcg agtccgcatc tttctttttg cgacggcgga ccggtgcggc
     5941 ggcctcggtt tcgggcggtg gctcgccggt gggcaggtgg aaatccagcc ggtcatcgcc
     6001 agcctcgact tccacgactt cgccttggct aaagttcccg cggaggattt cctcggccag
     6061 cggattttcc agccagcgtt cgacggcgcg gcgcagttgg cgcgcgccat aggcgcggtc
     6121 aaagcctttg ttgagcagga agtcgtaggc cgaagccagc acggagagct tgatctcgcg
     6181 cgccttgagg cgggcggcga ccttgccgac ctcaatttcg aggatggtgc ggacatcttc
     6241 gcgctgcaac tggcggaaca cgatgatttc ctccacgcgg ttgagcagct cgggacggaa
     6301 gttctgcttg gcggcttcca tcatctgctc gcgcagcgcg gcataatcgg cggcggcggt
     6361 atgctcggca aagcccagcc ccgccccctt gcgcgcggct tcggagccga tgttggaggt
     6421 catgatgatg atggtgttgc ggaaatccac ggcgcggccc aagccatcag tcaggcggcc
     6481 ttcttcgagg atttgcagca tcatgtgcat gacatcgggg tgggcctttt cgatttcgtc
     6541 gaacagcacg acggaatagg gacggcggcg gacgcgctcg gtgagctggc cgccttcttc
     6601 atagcccaca tagcccggcg gggagccgac gagccgcgag gcgttgaact tttccatgta
     6661 ttccgacatg tcaatctgga tgagggcgtc ggggctattg aacatgaact cggcgagcga
     6721 tttggccagc agcgttttgc cgacgccggt cgggccgagg aaaataaagg aaccaatggg
     6781 ccggcgcggg tctttgaggt ccgcgcgcga gcgacgcagc gccttgcaaa tcgaggaaat
     6841 ggcgtcctgc tggccgatga ccgtcttggc cagcgcggct tccatttcca gcaacttggc
     6901 ggtttccttc tgctccattt tttcgagcgg tacgccagtc cacttggaga cgatcaggcg
     6961 cacgtcctct tcgttgacat ccacaattcg ctcggtgtgg tcgcgccgcc agtccgccag
     7021 ctctttttcg agcgcggcgc gcatatcgcg ttcggcgtcg cgcaatttgg cggcctcctc
     7081 aaactgctcg ttttcgatgg ccaccttgcg ctgctccagc aagttggcga tttcgcgctc
     7141 cttctccttg acgtggacgg ggcgggtcat ggcgccgatc cgggcgcggg cgccggcttc
     7201 atcaatcacg tcaatggcct tgtcggggag gaagcggtcc tggagatagc gggcggagag
     7261 gcgcacggcg gcctcgatgg cgtcatcgcc aaagcgcgcg tggtggtggg cctcgtagcg
     7321 ggcgcgcagg cccttgagga tttcgatgga ttcctcgatg gacggctcgc gcacgaggac
     7381 gctctggaag cggcgttcca aggcggcatc tttttcgatg aacttgcggt attcgttcag
     7441 cgtagtggcg ccgatcactt ggagttcgcc gcgggagagc gcgggcttga ggatgttggc
     7501 cgcgtccatg gcgccctcgg cgctgccggc gccgacgagg ctatgcagct catccacgaa
     7561 cagaatcaca tttttgacgc ggcggatttc atccatgacc gccttgatgc gttcctcgaa
     7621 ttggccgcgg tattttgtgc cggccaccat cagcgccaaa tccagcgaga tgacgcgctt
     7681 gccgagaagc agttccggaa cttcgccgtt cacgatggcc tgggccagtc cttcggcaat
     7741 cgccgttttg cccacgccgg cttcgcccag caatgcagga ttatttttgg tgcgccggca
     7801 caggatgcgc atgacgcgct cgatttcatc ggcgcggccg atcaccgggt ccaacgcgcc
     7861 cttttgcgcc agtgcggtca aatcgcgtcc gaacgtagac agcgccggag ttttttgctc
     7921 tttggggcca cgggctttgt cgggcccggg gccgagttcg ggccccgggg catcgccggg
     7981 cgtttcctca tcggcgggcg gcgcctcgta attcggatcc agctccttga ggacggcatc
     8041 gcgggttttt tcgatatcca cgtgcaggtt ggtcagcacg cgtgccgcca caccctccgc
     8101 ctcgcgcagc aggcccagca ggatgtgctc ggtgccgacg taatccatgt tcatggcccg
     8161 cgcctcggtg ccggccagcg ctagcacttt tttcacgcgc ggcgtatagg gcacgttgcc
     8221 gacgatcttc atgtccgagc ccacgccgac tgccttttcc acctccatgc ggacggtatc
     8281 caagtcgagc cccagccggc gcaggacggt gacggcgacc ccttcgccca aggcaatcag
     8341 gcccagcaac aggtgttctg tgccgatata gccatggttg aagcgatccg cttcctgctt
     8401 ggccagttgc aatacgcgct gcgcgcgtgg cgtaaaatta ttcatgaggg ttttcctttt
     8461 gcgcgcgttt cggggtccgg cccttgagca tggtgcgcag catgcgggcg cgcgctttat
     8521 cccgctcggc gctgttcatg gcgcggccgg ccgccgcctg taaatgcgcg ggctgcaccg
     8581 ccagccacag ttcatccacc aactgcacat cgagcccgcg cacgagcccc aggaggattc
     8641 ccagccgcaa ttccgagagg cgctccagcg cctcttgcgc ggtcatgagg cgggcggcgg
     8701 tcaaaatgcc tttggcccgc gcggccaaat cttccacaaa gagccgccgt tcccgcttga
     8761 ggcgggcgcg ggcgttctgc tcgtggcctt ccagctcgcg tacgatttcg ccgaggtctt
     8821 ccacgatgcg ctcttcggtc aggcccagcg tgcgctggtt cgacacctga aacatgtggc
     8881 ccaaagccgc ggtgccctcg ccccagcgac cgcggaccgt caggccgatt ttcgccagcc
     8941 cgttggcgat gggctccatc tcgttctgca acacaagggc gggcaggtgc atcatggcgc
     9001 tggcacgcat gccggtgccg acattggtcg ggcaactggt cagatagccc agcgtggatg
     9061 aaaaagcgta gtccagtttt tgctccagga aatcgtccag atcgttggcc acctgccagg
     9121 ctttctccaa cgccagcccc ggttgcagca cttggatgcg caagtgatct tcctcattga
     9181 tcatcacggt gcaactgtgg gcggcatcca tgcagatggc ggattcgggc cgttcggtca
     9241 gcaagtggcg gctgatgaga tgcccttcga aaagcagttg gcggcgcagc gtcggcgcgc
     9301 cggccagcgc ttcttggagc ggcaatcgtt cccgcgcggc atccatcacg cgctggcaaa
     9361 cctcggcgcg cacggcgctg ggagcgcggt cagggaatgg cagaccgcgc aggttacgcg
     9421 ccaggcgcac ccggctgctc aacaccacgg cgtgggcgtc cgcatagttg gcggactcgc
     9481 tcacggctgc tcctttccgg cgctcaacgc ccggagctgg tcgcgcagca gggcggcttc
     9541 ctcatacttt tcagcggcga tggccttatc cagctcgcgg cggagccggc cggtttccac
     9601 cgagaggcgc aagccggagg aaattcgctt gggcaccttg cccacatgct ggtcgccgcg
     9661 ctgcagggac accaccacgg gcaccatttc ggcgccgaac acttcgtagc agcgatcgca
     9721 gccgagccgc ccttttttca gcacatcggt ccgcttcatg ccgcacaccg ggcaggcatg
     9781 ctggccggcc ggcggggtgt ccgccgccaa cgtctcctgc aactggtcca gttgattctt
     9841 caattcggcc atcatgccgg aaaggtccac ggttttgtcc gccagcggca tcttcaatgc
     9901 ggcggcacac tccgcgcaaa gggagagttt gaccacttgg ccgtcttccg ttttggtgaa
     9961 ctggaattgg gcctctttca ctttacattt ctggcatagc atcgcggcgc accctatcac
    10021 cgccacgcca gtttttccag ccccaaaccc gtgcgttttt tgctcgtgcc gagccaccgc
    10081 cgcgtgctac agtgacccca tgacgtggaa agacaccatg ggcgcgttgg tttccccgtt
    10141 gaaggcgtgc tttgacctca cgttggccga gcgacgcttt ttgctggggg tgctgctgct
    10201 gtttgccctt ggcctgggcg cgcgctggct gcacctgcgc agcgaccgct ccacgcccta
    10261 caccccaccc gcggagcaac gccatccatg agcaactgca ttttctgcaa gatcgtcgcc
    10321 ggcaccatcc ccgccaccaa gctgtatgag gatgatgatg tcctcgcgtt catggacatt
    10381 gggccgattg tcaaaggcca cgtgctggtg attcccaaaa cccacacgga gaccatcggg
    10441 caaacgccgc cggaaactct cgccaaggta atcgccgtgg tccggcgcat ggcgcgcgcc
    10501 cagcaggcgg gactcggcgc ggacgggatc aacattcacc agtccaacgg cgcggccgcg
    10561 ggccaggtgg tgccgcatat ccatttccac gtgatccccc gcttcaagca cgatggccac
    10621 cactggaact ggaccgcgca atcctatgcc gaccccagcg aagtggctgc tctcgccgac
    10681 cgcatcaagg ccgctctcga ctgagccaat gaaaagccca accttcgcag aaatcatcgc
    10741 ccggatttgc gcccgcgacc ggcgctacgc cgccgatgcg tttgtttttt tgagcgaagg
    10801 gctccaattc acggtcaagg ccatccagga aaaagagaaa aatcgccgcc atgtcacggg
    10861 ccaagaactg gcgctgggct tgcgtgatta cgcgctggaa caattcggcc cgctggccat
    10921 gacggtcctc gcgcgttggg gtgttcagac cacccgcgac tttggcgaga tggttttcct
    10981 gctgatcggg gccggcttgt tcggcaaaac cgaagaagac aaaatcgaag acttcgatga
    11041 cgtctacaac ttcgccacag ccttccgcac ccctttcctg cccaagctta ccgcccagcg
    11101 ccagccgcgc ccccgcccca caaccgcata accgcgccgc gagacggcgg tggccggggg
    11161 agaggtcagg gggcagaggt cagaagtcag aggtcagagg tcaggggaag gccaagaacc
    11221 ggaacagccc cggcctcagc gaggccggct acagtaactc tcggttacgg actttagcgt
    11281 ctcttgcgtc gattgcgtta aacttcttct tggcggcttg ggagagaagt tttccacggt
    11341 gtggaaaaat attttccacg gtgtggaact tttgccgttg atttttccac agtgtggaaa
    11401 aaatggccca aagttgttcc attgtgtgga aaagtcgggt caaagttgtt ccattgcgtg
    11461 gaaaaaattg ggtgattttt ccacggtgtg gaaaatcccg cccacgcgtg atgaactttt
    11521 ttccacggtg tggaaaacca tcgaacccag caccgccgca aggcggcgga ggggtcaggg
    11581 ttcagagttc aggtttcaag taaaaaccag cagccaacag ccccggcctc agcgaggccg
    11641 gctacagtaa gacaggaaaa ccaacagccc gccgccggta caccggcggc ctacagaaaa
    11701 ccggcagccc gccgccacga agtggcgcga ttatgaaccc gaaaaatgca tttgggcata
    11761 ggggttgcgt catcactcga tgaacactgg gtgatttgag ggtgttattc gtttggatca
    11821 atggtgacgc tc
//
LOCUS       DAOPNP010000077        11762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6298_length_11762_cov_8.548304, whole genome shotgun sequence.
ACCESSION   DAOPNP010000077 DAOPNP010000000
VERSION     DAOPNP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_11762_cov_8.548304"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..959)
                     /locus_tag="PKI64_07715"
     CDS             complement(207..959)
                     /locus_tag="PKI64_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOE00932.1"
                     /translation="MKISLRTIRRSVHLLLLLAGFAALLAAAVEFTDLPWRAYKHLSS
                     APGLSAEPPALILVMGGSGIPGDSGLMRTFYAAEAAHQFPAAQVLIAMPLDAPQSPAS
                     QAYMDELRLRGVAPDRLHILPDGRNTREQAIRMAEYLRNHPDQRHVLIVSSPEHIRRT
                     AASLRIAFAAARLDVVLGALPAFPLSLEDPLPWRAQDLDTPATATLIQAAVPDLGSSL
                     HLRYQLWSNLRYTLDSLREITALLYYRLRGWA"
     gene            1081..3096
                     /locus_tag="PKI64_07720"
     CDS             1081..3096
                     /locus_tag="PKI64_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOE00933.1"
                     /translation="MKKWVRALVSVGVSAGIVILVLRAAGTSAGEVLLAVANLGLVTW
                     GLYIAAQLGQGWLRAVRYRLLLGGAGVEPPGAARMFGVALARNMFVDMLPARAGELMY
                     WALLNRGEGVRNEACVSSMTISVLFDFVALALVLAGVLVAPLATAGGRGILVWSMGGV
                     LLVVLVGAVALSIGPAWAAAVFRRLPARWQQWRAVAWVEEFMDKLTDSFRQVGRSGVL
                     WRAAGLSVGIRAVKYLGLLAAFVGVAGILRPALAGLPVWQVLVGLISGEGGAALPVPT
                     FLSMGSYEAAGAGALRLTGVSAADAAIVLLGTHVASQVVDYALGGLGLLGLLWGGRKR
                     VAWAAGIALLLLVLAAGGAVKWRAQQKAGAREAPPAGEVLPMEPEARAALERAWGGRT
                     GFIVWSSTMFGNHELVRLDWPSGQITRLTQNGVVDSTPKISPDGRQVVFARSRREWVS
                     CRNFTDWDIWVLDLASGKEHRVAEFGVEPSWTGDGRAVVFQRGGQSVLQVDLQTGKEA
                     VLLAPQGKLQWTGPSMDPRGGRVAVTVRGGRRGTSLFSVPDGHETRVAGGCQLAFVPG
                     GEWLVLVEDGGRLKNRICRVDRDGRNVQTLLDMPGEWSHEYFPRVSNDGGLLVFGASR
                     GDHEHDTADYEIFLWPLGAPPEQAARVSFHTGNDQWPDIWVQPAAQP"
     gene            3164..>4476
                     /locus_tag="PKI64_07725"
     CDS             3164..>4476
                     /locus_tag="PKI64_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE00934.1"
                     /translation="MTTQTTRIVPEREPSLAAEQQPRVMRVGTMLLDRYEVLAVLGSS
                     QLGEVWQCFDRHESRHVAIRWLPTDLQRSKRLLNAIHEGIRRLSDKTHDNIAVIRRLM
                     HVGEQTYVVGDYAPGMDIGMWSRAGTDGRRTLTEALPILGQIAAGLDFVHQQRVVHGN
                     LKPSNVFLGPDGVVRITDFGLTPRGHLVFVRGEIQRTGSTGAYLAPEIRTADDEPDAG
                     SDQYALAVLACEMLGGPELNPTKLVPGDLPATARAALARALSRKPRNRFASCDDFVRA
                     LGGERIARRRGRSAAEWQRIKNHLILSASTVAMAAVLGVIGYITVEVMKRPKPEPAPI
                     KLSAPAPLPAKKAEPAGPTVPRVPPLVITTEMPTPGQPWATQTGNMEFVWVPGLSMWV
                     GRFEVTNDQYKLKDPEHDSGDFRELPLTGPRQPVVRINFEDCVEYA"
     assembly_gap    4477..4576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4577..4832
                     /locus_tag="PKI64_07730"
     CDS             <4577..4832
                     /locus_tag="PKI64_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00935.1"
                     /translation="GAGGNVWETTSKVPGGKEFGGWQGGGWDDHLPSRMGCEVTYGFL
                     GNARGAVNGLRLVLAPIADEAPPPPAAQAPATAAVTPTSG"
     gene            5417..5725
                     /locus_tag="PKI64_07735"
     CDS             5417..5725
                     /locus_tag="PKI64_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HOE00936.1"
                     /translation="MQEKGFFELLFDFSFSEFVTTKIIKVLYVLAIIGAGIAALTTLF
                     SGFATKSFSGVLYGLVMTPIVFLLGTLVARIWLETLIVLFRIAENTTKLVRGHEPPTS
                     "
     gene            complement(5760..6524)
                     /locus_tag="PKI64_07740"
     CDS             complement(5760..6524)
                     /locus_tag="PKI64_07740"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOE00937.1"
                     /translation="MKKPLREQAIEYLRYQKEQGVTHIPTTRAWKPRPPAAATPGRPS
                     PISASVPPATPRTGALAPLVQSGSPELDAITAEIAACQRCPLAATRLNTVPGQGALHP
                     DIMFIGEGPGADEDAQGRAFVGRAGQLLTKMIAAMGYTREQVFIGNIVKCRPPGNRVP
                     TPEEMAGCIPYLKRQIAIIKPKVIVCLGATAARGLVEEVLPIGKARGQWREFEGVPVM
                     LTFHPAYLLRDPSKKGLCWADLKAVLARLGKEPPRA"
     gene            6583..7251
                     /locus_tag="PKI64_07745"
     CDS             6583..7251
                     /locus_tag="PKI64_07745"
                     /inference="COORDINATES: protein motif:HMM:NF025780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
                     /protein_id="HOE00938.1"
                     /translation="MRILPGAEMVGFWSKPHESQLTTRPFLMISQPLQRIGRHLAGWA
                     LLLLLAVNVIAAPPARQPAIRQIDAKRDRTVLQRIEQVRQKLPEKYQHRNNFAWAAVK
                     IAGVEKTEYFAHSGIQRQSDVSAEAWAGISVISLRCRKGRFTVLCVNHNDEIEGENCW
                     PRHVDTECKILEDLAARIPLPVARGQVLLYTDLYPCASCRYVMEQFLAAFSNVTLQVL
                     FREY"
     gene            complement(7269..7913)
                     /locus_tag="PKI64_07750"
     CDS             complement(7269..7913)
                     /locus_tag="PKI64_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE00939.1"
                     /translation="MPPQLFLFDLDGTLIDSRADIAAAVNQMRARHDLPPLPLERVVT
                     YVGDGVRVLAQRALEGAPVELDAAVNEISAAYAADLTSRTTPYPGVTDGLRALHSAGH
                     HLGLVTNKPGPHARRLLDHFGWTPLFSVALGGGDTAELKPSPLPLQTAMLRAGHAADT
                     TWMVGDHHTDLAAARRAGVRAIFLANGFGNTGTEQADWTFPDFAAFTAHFLASP"
     gene            complement(8004..8882)
                     /locus_tag="PKI64_07755"
     CDS             complement(8004..8882)
                     /locus_tag="PKI64_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE00940.1"
                     /translation="MTYWLYRALAFLIRLLPLKLAYWIGLRICDLFYFLNRRGARAVH
                     EHIRIIFAHQGIIPSRQILAGCTRKTFQAFGKYLADFIRFRKLTEQEVRDSISIQGLE
                     NIERIRQEKRGAIFLTAHYGNWELGGSFIASLGIPLNAVVRPVSSPSLERIFHYFREQ
                     RGLKVIPLAHAGVGVIKALKRGEAVALVGDRDFTGNGRPQTFFGREVSLPRGAAWFAH
                     RTGAPLYLGFVTRAPDDTFILRIHPPIDPTEVRTEEEIQERVIAIMQDTIARDPTQWF
                     IFDPFWTHEFAAAPSA"
     gene            complement(8879..10012)
                     /locus_tag="PKI64_07760"
     CDS             complement(8879..10012)
                     /locus_tag="PKI64_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00941.1"
                     /translation="MSRPPPRILICYLVRNSGHHAAARAIEAEIRKQSPHAEIRCLDL
                     LETTHPTWSALVQYAYMSTIRRTPELWEALYDNFWFDWLTKWIRPLVQRGNSLKLRIL
                     MDAFQPDAMVCTQAHPFAVLSAYARRQGQPIPLWGVVTDFLPHRFWFVPPNEHIRYVV
                     STETAVQRLMILGIPGERIHTFGIPIQADQPADRKRPTRLDRRRVLVMGGSRGLGARF
                     ATIRHLDRATSDFTIDVVTGTNRRLRKQLLGARHKFKHPMRIRGYVRNVAAMMRHSSL
                     LIGKPGGLTSAEAMVAGTPMLIIRPLPGQERGNTEMLVRHGAAIHLDRDRDLPAVVTS
                     LLTNPTLLDMMAARARALGKPDAARDIANAVLAHIPPEAAPAP"
     gene            complement(10009..10869)
                     /gene="lipA"
                     /locus_tag="PKI64_07765"
     CDS             complement(10009..10869)
                     /gene="lipA"
                     /locus_tag="PKI64_07765"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOE00942.1"
                     /translation="MPARLPNWFRRPLLPGTTGSRVKQLLGQHGLHTVCEGAKCPNRA
                     ACWHDAAAAFLILGDTCTRDCGFCAIPHGAVLPPPDPGEPQRLAEAAAGMGLRYVVVT
                     SVTRDDLPDGGAAHFAATIHALRARIPGVEVEVLIPDLQGDTTALDIVLAAQPDILNH
                     NLETVARLQPTVRPQADYARSLGVLRYAAQAGARVKSGLMLGMGETREELMAALHDLR
                     AAGVTLLTLGQYLAPSAAHHPVARFVPPEEFAALHEQALALGFVAVAAGPQVRSSYHA
                     GELVARGKVS"
     gene            complement(10878..>11762)
                     /locus_tag="PKI64_07770"
     CDS             complement(10878..>11762)
                     /locus_tag="PKI64_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOE00943.1"
                     /translation="QDVIKKHEFTRKDKEDDRTKHTAVLNANIGPVFIAYRDVPAIRD
                     ALAPIPERTAPLYDFVDELNVRHRVWPVADPAPIVKLFAEQVPISYIADGHHRSASAV
                     RVGTERRAANPQHTGAEEYNWFLAVLFPGSALQILPYNRAVKDLNGLASDAFLAKIKE
                     LFTVTENPNPSPQNPCQISMYLGGKWYGLSWPAVTADNPVDRLDVSTLQNRVLKPLLG
                     IDDPRTSQRISFIGGIRGPQELVRLVDQKGYAVAFSMYPTTVDQLMAIADAGQIMPPK
                     STWFEPKLRTGLLSHELN"
BASE COUNT         2128 a         3076 c         4267 g         2191 t
ORIGIN      
        1 atgcatgaac gaaaccccat agcatctaca attcagccgg aatcagcttt ttgggccggg
       61 acaccccaaa acgaaatcgt ccggttgcaa tgcaacgagt gacaacgaga ttttcggggt
      121 atgcggaact caagaaagct ttcctcgggg tttgaagcga tttttttggg ggaagcattg
      181 agatggggga agctggattt gagttgctag gcccagccgc ggaggcggta gtagaggagg
      241 gcggtgattt cgcggaggga gtcgagggtg tagcgcaggt tgctccagag ttggtagcgc
      301 aagtggagag aggaaccgag atcagggacg gcggcttgaa tgagcgtggc ggtggcgggg
      361 gtatcgaggt cttgggcgcg ccacgggagg gggtcttcga gggataacgg gaaggcggga
      421 agagcgccca agacgacgtc cagtcgggcg gcggcgaagg ctatgcgcag ggaggcggcg
      481 gtgcggcgga tatgttcggg gctgctgacg atcagcacat ggcgctggtc aggatgattg
      541 cggagatatt cggccatccg gatggcttgt tcgcgggtat tgcggccatc gggcagaata
      601 tgcaaccgat caggggcaac accgcgcaag cgcaattcat ccatataagc ctgtgatgcg
      661 ggggactggg gggcatccaa cggcatggcg atgagcactt gggcggcagg gaattggtgc
      721 gcggcttcgg cggcgtagaa ggtgcgcatg agaccggagt cgccggggat gccgctgccg
      781 cccatcacca gaatgagggc gggtggttcg gcgctcaagc cgggtgcgct ggaaaggtgc
      841 ttgtaggcgc gccagggcag gtcggtgaat tcaacggccg cagcgagcaa cgcggcgaag
      901 ccggcgagca gcaggaggag gtgcacggag cggcgaatgg tgcgcaatga gattttcatg
      961 ccggcatgtt tcccgaaaaa atgggcgagg ggaaggctat ttgtatcggc gcggggagta
     1021 agcagtgacg tgcaggattt acggggtagc ataaatggcg ggttgtatta tgatggcgcc
     1081 atgaaaaaat gggtgcgagc gttggtgtcg gtaggcgtat ccgccggcat tgtgattttg
     1141 gtgttgcgcg cggcgggcac atccgcgggc gaagttttgc tggcggtggc gaatctgggg
     1201 ctggtgacgt gggggctgta tattgccgcg caactagggc aggggtggtt gcgggcggtg
     1261 aggtatcgct tgttgctggg gggggcgggg gtggagccgc caggtgcggc ccggatgttt
     1321 ggtgtggcgt tggcgcgcaa tatgtttgtg gacatgttgc cggcgcgcgc gggcgaactg
     1381 atgtactggg cgttgctgaa tcgcggcgag ggggtgcgca acgaggcttg tgtgtcatcc
     1441 atgaccatca gcgtgctgtt tgattttgtg gcgttggcgc tggtgttggc gggagttttg
     1501 gtggcgccgc tggcgacggc gggcgggcgc ggtatcttgg tctggagcat ggggggcgtg
     1561 ctgctggtgg tgctggtggg ggcggtggcg ttgagcatag ggccagcgtg ggcggccgcg
     1621 gtattccgcc ggctgccagc gcgctggcag cagtggcggg cggtggcatg ggtggaggag
     1681 ttcatggata aactgaccga ttcattccgg caggtggggc ggtcgggggt gctttggcgg
     1741 gcggcggggc tgtcggtggg aattcgcgcg gtgaaatacc tcggcttgct ggcggcgttt
     1801 gttggggtgg cgggcatatt gcgcccggcg ttggccgggt tgccagtgtg gcaggtgttg
     1861 gtgggattga ttagcgggga ggggggggcg gcgctgccgg tgcccacttt cctgagcatg
     1921 gggagctatg aagcggcggg cgcgggcgcg ttgcggttga cgggggtgtc ggccgcggat
     1981 gcggcgattg tgctgctggg aacccacgtg gcgagccaag tggtggatta tgcgttgggc
     2041 gggctgggtt tgctggggct gctttggggc gggcgaaagc gagtggcgtg ggcggcggga
     2101 atcgcgctgc tgcttttggt gttggcggcc ggcggggcgg tgaagtggcg cgcgcagcag
     2161 aaggcaggcg cgcgagaggc tccaccggcg ggggaggtgc tccccatgga gccggaggcg
     2221 cgggcagcat tggaacgggc gtggggcggg cggacgggtt tcattgtgtg gagttcgacg
     2281 atgttcggca atcatgagtt ggtgcggttg gattggccgt cgggccaaat cacgcggctg
     2341 acgcaaaacg gggtggtgga cagcaccccc aagatttcgc cggacggccg gcaggtggtt
     2401 tttgcgcggt cgcgccggga gtgggtgtcg tgccgcaatt ttacggactg ggatatctgg
     2461 gtgttggatt tggccagtgg caaggagcac cgggtggctg agtttggcgt ggagcccagt
     2521 tggacggggg atgggcgggc ggtggtgttt cagcgcggag ggcagagcgt gttgcaagtg
     2581 gatttgcaaa ccggcaaaga ggctgtgctg ctcgccccgc aaggcaaatt acaatggacc
     2641 gggccgtcaa tggacccgcg gggcggacgg gtagccgtga cagtgcgcgg cgggcgtcgc
     2701 ggcacatcgc tcttcagtgt gccggatggc catgaaacac gtgtggcggg ggggtgccaa
     2761 ctggcgtttg tgccgggtgg cgaatggctg gtgttggtgg aagatggcgg gcgattgaaa
     2821 aaccggattt gccgggtgga ccgcgacggt cgcaacgtac agacgctgct ggatatgccc
     2881 ggggagtgga gccacgaata cttcccgcgc gtatccaacg atggcgggct gctcgttttt
     2941 ggtgcgtcgc gcggcgatca cgaacacgac acggccgatt atgagatttt cctgtggccg
     3001 ctgggcgcgc cgccagagca agcggcccgg gtatccttcc acactggcaa tgaccaatgg
     3061 ccggatatct gggtgcagcc agccgcccag ccatgacaaa atggaccgta ttttacggat
     3121 ttgattgtcc gcagcgcggg aaaatgacag tattgggctg atcatgacca cacagacgac
     3181 caggattgtt ccggaacggg agccctctct ggccgcagaa cagcagccgc gcgtgatgcg
     3241 ggtgggcacg atgctgctgg atcgctacga agttctggcg gtgctcgggt cgagtcagtt
     3301 gggagaagtg tggcagtgct ttgaccgcca tgagtcgcgc catgtggcca tccgctggct
     3361 gccgacagac ttgcagcgtt ccaagcggct tttgaatgcc atccatgagg gcattcgccg
     3421 gctttcggac aaaacgcacg acaacattgc ggtgattcgg cgtttgatgc acgtgggcga
     3481 gcagacctat gtggtgggtg attacgcgcc ggggatggac atcggaatgt ggagtcgcgc
     3541 gggcaccgat ggccggcgca cgttgacaga agcgttgccg atactggggc agatcgcggc
     3601 agggctcgat tttgtacacc agcagcgcgt ggtgcatgga aacctcaagc caagcaatgt
     3661 gttccttggg ccggatggcg tggtgcggat tacggatttc gggctgaccc cgcgcgggca
     3721 cctagttttt gtacgcggcg aaatccagcg gaccggctct accggcgcgt atttggcccc
     3781 agaaattcga acggccgacg acgagcccga tgcgggcagt gaccagtatg cgctggccgt
     3841 gttggcctgc gaaatgctcg gcgggccgga acttaatcct accaagctgg tgccgggcga
     3901 cttgccggcc acggcccgcg ccgcgttggc ccgggcattg tccaggaagc cgcgcaaccg
     3961 ctttgcctcg tgcgatgatt ttgtgcgcgc gctgggtggc gaacgaattg cgcggcggcg
     4021 cggacgcagc gcagccgaat ggcaacgcat caagaaccat ttgattctgt ccgcctcgac
     4081 cgtggccatg gccgcggtac tgggagtgat tggttacatt accgtggaag tcatgaagcg
     4141 gcccaagccg gagcccgcgc ccatcaaact atccgcaccc gcccctttgc cggccaagaa
     4201 agccgaaccg gccggcccga ctgttccgcg cgtgccgccg ctggtcatca ccacggagat
     4261 gccgacgcca ggccagccgt gggccacgca aaccggcaac atggagtttg tgtgggtgcc
     4321 gggattaagc atgtgggtgg ggcgctttga agtgaccaac gaccaataca aactcaagga
     4381 ccccgaacat gacagcggcg atttccgcga attgccgttg acggggcccc gccagccggt
     4441 ggtgcgcatt aattttgagg attgcgtgga gtatgcnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnntggc gccgggggca acgtgtggga aaccacatcc aaggtgccgg
     4621 gcggcaagga gtttggcggc tggcaggggg gggggtggga tgatcacctc ccttcgcgga
     4681 tgggctgcga agtgacctac ggcttcctgg gcaacgcacg gggggcggta aatggtctac
     4741 ggttggtgct ggcgcctatt gctgacgaag caccgccgcc gcccgctgca caggcacctg
     4801 ccacggctgc cgtcacgccg acatcgggat aattccgact acctcgttgc gtcaaatgaa
     4861 aacgacatcg aaatatacgc cgcaggggta gattcgtgag aagggtcacg tttcttactc
     4921 ctcttgccgc ccctgtcagc ttgagccctt tctgaacccg tccacgcggg ggcggttgcc
     4981 aattttctag tggtcccctc gagctctttg gacacaaggc gtacactagg aatgtagaga
     5041 gttttgatga aaccatccaa ggcattgatc tcattcaaga ggtcaatcag tccgggaact
     5101 acgatccggc catggccgga caacgtgcgc gctctggctc cgttcttttg ctcaacatgg
     5161 gtgttgtcgc tcttgcaata accgcatgac cgggaacggc agacttgccg cacgtgctcc
     5221 actcagtgct gtgcgatatg agtatagagt acttccccgg tgttgtctga gtttaggaag
     5281 agcgagggga acggcatggc gctgctcatg caccttgtgt ggccgtggcc gacctgaatt
     5341 tgcgggacac cgcaagatgc gctacggtgt ggacgtggtt cgccacgacg gcaacgcata
     5401 aaacaaggag aaggccatgc aagaaaaggg attctttgaa cttctgtttg atttttcatt
     5461 cagcgagttc gtcaccacca agatcatcaa ggttctgtat gttctggcga tcatcggtgc
     5521 gggcattgcg gctttgacga cattattcag cggctttgcc acgaaatctt tttctggcgt
     5581 gctgtacggc ttggtgatga cccccattgt gttcttgttg ggcaccttgg tggcgcgtat
     5641 ttggctggaa acgctaattg tgttgttccg cattgcggaa aacaccacga agctggtgcg
     5701 cggtcacgag ccgccgacca gctagcgttt gctcaccggc gtgctggcgc ctatcccagt
     5761 caagcgcggg gcggttcttt cccgaggcgg gcgagaactg ccttgagatc ggcccagcac
     5821 aatccttttt tggaaggatc gcgcaggaga taggcggggt ggaaggtcag catgacgggc
     5881 acgccttcaa actcccgcca ctggccgcgg gccttgccga tgggtaggac ttcttctact
     5941 aagccgcgcg cggctgtcgc gccgaggcac acaatgacct tgggtttgat gatggcgatt
     6001 tggcgtttga gataggggat gcagcccgcc atttcttccg gggtgggcac gcggttgccg
     6061 ggggggcggc atttgacgat gttgccgatg aacacctgct cgcgggtgta gcccatggcg
     6121 gcaatcattt tggtgagtag ttgaccggcg cggcccacga aggcacggcc ttgtgcgtct
     6181 tcatcggcgc cggggccttc gccgatgaac atgatgtcgg gatgcaacgc gccttggccg
     6241 ggcacggtgt tgagacgagt ggctgccaaa ggacagcgct gacacgcggc aatttccgcc
     6301 gtgatggcat ccagctcggg cgagccgctt tgcaccagcg gggccagcgc accggtgcgc
     6361 ggagtggccg gcggcacgct cgcggagatc ggagacgggc ggcctggcgt ggcggccgcg
     6421 gggggacgtg gcttccacgc ccgcgtggtg gggatgtgcg tgacgccctg ctctttttgg
     6481 taacgcaaat attcaatggc ctgttcgcgc aggggctttt tcatggggca gcattttagg
     6541 ccagcggcgc gaaataacga atgaaaaaat ggggtgcaac aaatgcgtat cttaccgggg
     6601 gcggagatgg taggcttctg gagtaagccc catgaatcac aactcaccac gcggccattt
     6661 ctcatgattt cgcaaccgtt gcagcgaatt ggacggcatc tggccggctg ggcgttgttg
     6721 ctgctgctgg cggtgaacgt aatcgctgcg ccgccggccc gccaaccggc cattcggcaa
     6781 attgacgcaa agcgcgaccg gacggtgctg cagcgcatcg aacaggtacg gcaaaagctg
     6841 ccggaaaaat atcagcaccg gaataatttt gcgtgggcgg cggtaaagat cgcgggcgtg
     6901 gagaaaaccg aatacttcgc gcacagcggc attcagaggc aatcggatgt ttcagcggag
     6961 gcgtgggccg gcatcagcgt catttctttg cgctgccgca aagggcgctt cacggtgttg
     7021 tgcgtcaacc acaacgatga aatcgaaggc gagaactgct ggccgcgcca cgtggacacc
     7081 gaatgcaaaa ttctggaaga cctcgccgcg cgcattcccc tgcctgtcgc gcgcgggcag
     7141 gtgctgctct ataccgatct ttatccgtgc gccagttgtc gctatgtgat ggagcaattt
     7201 ttggcggcgt tcagcaatgt gacactgcag gtgctattcc gcgaatattg agcgcagggg
     7261 cgggggcgct acggcgaggc aagaaaatgc gcggtgaacg cggcaaaatc ggggaatgtc
     7321 caatcggctt gttccgtgcc ggtgttgccg aatccgttgg cgagaaaaat ggcgcgtacg
     7381 ccggcgcggc gcgcggcggc gagatcggta tgatgatcgc ccaccatcca cgtggtgtcc
     7441 gccgcgtggc cggcgcggag catggcggtt tgcaacggca gtggggaggg cttcagctct
     7501 gccgtgtcgc caccgcccaa cgccacgctg aagagcgggg tccacccgaa gtggtcaagc
     7561 aggcgtcgtg catgggggcc gggtttgttg gtgacgaggc ccaagtggtg tccggcggaa
     7621 tgcagcgcgc gcaggccgtc cgtcacgccg gggtaggggg tggtgcggct ggtgaggtcg
     7681 gcggcatagg ccgcggaaat ttcattcacg gcggcatcga gttccacggg cgcgccttcc
     7741 agcgcgcgct gagccagcac gcgcacgcca tcgcccacat aggtcaccac gcgttccagc
     7801 ggcaggggcg gcaaatcgtg gcgcgcgcgc atctggttga ccgcggcggc aatatccgcg
     7861 cgcgagtcaa tcagcgtgcc atccaaatca aacaggaaaa gttgcggcgg caattttcca
     7921 tggtgtggaa aaacattttc catagtgtgg aaaactataa gccatccgcg aggaatccca
     7981 aaccggaaag tccgcgcggc ccatcaggcg gaaggcgccg cggcaaattc gtgggtccag
     8041 aagggatcaa aaatgaacca ctgcgtgggg tcgcgcgcga tggtgtcttg catgatggca
     8101 atgacgcgct cctggatttc ttcctcggtg cggacttcag ttggatcaat gggggggtgg
     8161 atgcgcagga taaaggtgtc gtcgggggcg cgggtgacaa agcccagata aagcggcgcg
     8221 ccggtgcggt gggcaaacca tgccgcgccg cggggcaggg agacttcgcg cccgaaaaag
     8281 gtttgcgggc gaccgttgcc ggtgaagtcg cggtcgccca cgagcgccac ggcttcccca
     8341 cgtttgagcg ccttgatgac gccaacgccg gcatgggcga gcggaatgac cttcaggccg
     8401 cgctgctcgc ggaagtaatg gaagatgcgt tccagcgatg gggaggagac gggccgcaca
     8461 acggcattga gcggaatgcc cagcgaggca atgaaggagc cgcccagttc ccagttgccg
     8521 tagtgcgcgg tgagaaaaat tgcgccgcgt ttctcctggc gaatacgctc aatgttctcc
     8581 aagccctgaa tgctgatgga gtcgcggact tcctgttcgg tgagtttgcg aaagcgaatg
     8641 aaatcggcga gatatttgcc gaaggcctga aaggttttgc gggtgcagcc ggccagaatc
     8701 tgccgggagg ggatgatgcc ctgatgggcg aaaatgatgc ggatgtgctc gtgcacggcg
     8761 cgcgcaccgc gccggttgag gaagtagaac aagtcacaga tgcgcagccc gatccagtag
     8821 gcgagtttca gcggcagaag ccggatcagg aaagccagcg cgcggtacag ccagtaggtc
     8881 atggggcggg cgcagcctcg ggcgggatgt gggcgagaac ggcgttggcg atgtcgcggg
     8941 cggcgtcagg cttgccgagg gcgcgggcgc gggcagccat catgtccagc agcgtggggt
     9001 tggttaggag cgaagtcacc acggcgggca aatcgcggtc gcgatcgaga tggatggccg
     9061 caccgtgacg caccagcatt tccgtattgc cgcgttcctg gccggggagg gggcggatga
     9121 tgagcatggg agtgcccgca accatggcct cggcagaggt gaggccgccg ggcttgccga
     9181 tcagcaggct ggaatggcgc atcatggccg cgacattgcg cacatagccg cgaatacgca
     9241 tcgggtgctt gaatttgtgg cgggcgccca gcagttgctt gcgcaggcgg cggttggtgc
     9301 cggtgacgac atcaatggtg aagtcgctgg tggcccggtc gagatggcgg atggtggcga
     9361 accgcgcgcc gagcccgcgc gatccgccca tgaccagcac gcggcggcgg tccagccgcg
     9421 tggggcgttt gcggtcggcg ggctggtcgg cttgaatggg aatgccgaag gtgtggatgc
     9481 gttcgccggg aatgccgaga atcatcaggc gctggacggc ggtttcggtg gaaaccacgt
     9541 agcggatgtg ctcgttgggc ggcacgaacc agaagcggtg tggtagaaaa tcggtcacca
     9601 cgccccacag cggaatgggt tggccctgcc ggcgggcata ggcggagagc acggcgaaag
     9661 gatgcgcctg ggtgcagacc atggcatcgg gttggaaggc atccatcagg atacgcagct
     9721 tgaggctgtt gccgcgttgc accagtgggc ggatccactt ggtgagccaa tcgaaccaga
     9781 agttgtcgta gagggcttcc cacaattcgg gagtgcggcg gatggtggac atataggcat
     9841 attgcaccag cgcgctccac gtagggtgcg tggtttcgag caaatcaagg cagcggattt
     9901 ccgcgtgggg gctctgcttg cggatttcgg cttcgatggc gcgcgcggcg gcatgatggc
     9961 cgctgttgcg cacgagatag caaatcaaga tgcgcggcgg ggggcgactc atgagacctt
    10021 tccgcgggcg accaattcgc cggcgtgata cgaggagcgc acctgcgggc cggccgccac
    10081 ggccacaaag cccaacgcca gcgcctgttc gtgcagggca gcgaattcct cgggcggcac
    10141 aaaccgcgcc acgggatgat gggccgcgga aggcgcgagg tattgcccga gcgtcaacaa
    10201 cgtgacgccc gcagcgcgca aatcatggag cgcggccatg agttcctcgc gtgtttcgcc
    10261 catgcccagc atgaggccgg atttgacgcg cgcgccggct tgtgcggcgt agcgcaacac
    10321 gccgagcgac cgcgcgtaat cggcttgcgg ccgcacggtc ggttgcagac gggcgacggt
    10381 ttccagatta tggttgagaa tgtccggctg ggcggccaag acgatgtcca gcgcggtggt
    10441 gtcgccttgc aaatcgggaa tgagcacctc aacctccacg ccggggatgc gcgcgcgcag
    10501 cgcgtggatg gtggcggcaa aatgcgccgc gccgccatcg ggcaggtcat cgcgcgtgac
    10561 ggaagtgacc accacatatc gcaggcccat gccggccgcc gcttcggcaa ggcgctgtgg
    10621 ctcgccgggg tccggcggcg gcaacaccgc accgtggggg atggcgcaaa aaccgcaatc
    10681 gcgcgtgcaa gtgtcaccca gaatgaggaa cgcagcggcg gcatcgtgcc agcacgcggc
    10741 gcggttcggg cacttggcgc cctcgcacac ggtgtgcagg ccgtgctggc ccaacagttg
    10801 tttcacgcgg ctccccgtag tgccgggcag cagggggcgg cgaaaccagt tgggcaggcg
    10861 cgccggcatg gtgttgctca gttgagctcg tgcgagagca agccggtgcg cagcttgggc
    10921 tcaaaccatg tgctcttggg cggcatgatt tggccggcat cggcgatggc catgagctga
    10981 tccaccgtgg tcggatacat cgagaacgcc acggcgtagc ctttttggtc cactaggcgc
    11041 accaactcct gcgggccacg aataccgcca atgaacgaaa tgcgctgact ggtacgcggg
    11101 tcatcaatgc ccagcagcgg cttgagcacg cggttttgca gcgtgctgac atccagccgg
    11161 tccaccgggt tgtccgcggt gacggccggc cacgagaggc cataccattt gccccccaga
    11221 tacatgctga tctgacaagg attttgcggg ctgggattgg gattttcggt gacggtgaac
    11281 agctccttga ttttcgccag gaacgcatcc gatgccaggc cgttgaggtc cttgaccgcg
    11341 cggttatacg gcaggatttg cagcgcggag ccggggaaca aaacagcgag gaaccagttg
    11401 tattcttcgg cgccggtgtg ttgcgggttg gctgcacgac gttcggtgcc cacgcgcacg
    11461 gcggaggcgc tgcggtggtg gccatcggcg atgtagctga tgggaacctg ctcggcgaac
    11521 agtttcacga tgggcgcggg gtcggccaca ggccacacgc ggtgccgcac gttgagctca
    11581 tccacaaaat catacagcgg ggcggtgcgt tcgggaatgg gcgcgagcgc gtcgcggatg
    11641 gccggcacat cgcgataggc aatgaacacc gggccgatgt tggcgttgag caccgcggtg
    11701 tgcttggtgc ggtcgtcttc cttgtccttg cgcgtgaatt cgtgcttttt gatgacgtcc
    11761 tg
//
LOCUS       DAOPNP010000078        11634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6389_length_11634_cov_8.720960, whole genome shotgun sequence.
ACCESSION   DAOPNP010000078 DAOPNP010000000
VERSION     DAOPNP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6389_length_11634_cov_8.720960"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..931)
                     /locus_tag="PKI64_07775"
     CDS             complement(170..931)
                     /locus_tag="PKI64_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOE00944.1"
                     /translation="MVNPWVTGAMGWAFGGPLGGLIGYAAGTLINALAENATKAAKPP
                     PRPPRSAPRTSAGADRHNFAVSLLALTAAMMQADGRAMRSELIVAQKFFIQQFGRNLA
                     QTLMPILRDLLREEIPVEIVCRQIRANMSNPQRLTLLHYLYSLAYADGHLHPNEERLL
                     DLIARTLGIRPADRDSIAAMFAPRGDKYADYRVLEVAPDATDDEIRKAYRRLSMKHHP
                     DKVATLGPEFQRAATEKFQRINAAYSAIKKARGMT"
     gene            complement(924..4223)
                     /gene="mfd"
                     /locus_tag="PKI64_07780"
     CDS             complement(924..4223)
                     /gene="mfd"
                     /locus_tag="PKI64_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOE00945.1"
                     /translation="MAAREQPLNVPPLLAATRDYVARALAPGGHGELAILDDSARAWL
                     ALGAAQVAPGMLVVVAENTMALELLWQDVQTLGGGLPMVKLPALNVEASKLITGQPSP
                     DVSGERMNTLRQCLAWQGRGLLVTCVQALLEPVATPEQLQQDTLHLREQQECDLEQLC
                     GKLVAAGYAFESEVLAKGQASRRGGLLDVWPLTEDWPVRLEFFGDVLEGLRSFDPAEQ
                     RSRDKLREVWLPPARDAAADGPAAAPTDYVPADASFLWLDNEAITHHYAMSAELAGSG
                     ETALAAERDYANWQLMLARRCAGGGQLTAGGADLAHDSGAHPVAGLAALTAQAVPPDV
                     LETERQKWFAQLHERAAAGWTVDIFFATDAGRRRFAASFRKTKGIHLRLGGLSGGCEV
                     PAAQWMAVAEADLYGARRALPPRRKPRGGAARDATGERLTDWSDLRPGELVVHVEHGI
                     GKYLGLYEIVFDGQLQEALAVEYADEARLYVPTSQTHLLSRYIGVGRTKPPLHTLGGK
                     RWTKEKIAAARAANDLAAQMLETQARRAALQGHAFAPDTPWQHDFEQAFPFPETDDQL
                     RAIADVKRDMEAPQPMDRLICGDVGYGKTEVALRAAFKAVMDGKQVAVLVPTTVLAQQ
                     HYETFQERMGRFPITVEMVSRFRSRAEQAATLVRLAEKKVDVLIGTHRLLQRDVRFAD
                     LGLVIIDEEQRFGVAAKEHLKRLRELVDVLTLSATPIPRTLYLSLTGARDMSLIQTPP
                     RERLAIETYIAQDSDELLREVILREVNRGGQVFLLHNRVRTIEWMRERLARLLPGIRV
                     GVGHGQMKERQLADIMRRFVRREFDVLLCTTIIESGVDIPNVNTILIDRADRFGMADL
                     YQLRGRVGRSRHQAYAYLLLPRHGALFNAARRRISAIRRHTSLGAGFKLALRDLEIRG
                     AGNVLGAAQSGHIAAVGFDLYCQLLNRTIARLKGAPERPLIDVQLHLDFLDLTPDPAH
                     EAQAAVIPRNYVEDESQRIGLYRKLAGVAYASEVRDLQAEFRDRYGPLPAPVRRLLAC
                     ARIRLLAAALGVKSVATDGNRLLLGLGADEYLMVKGRHLRLHQHDPDDRLAEIEKRLR
                     QLVAAKEKSDG"
     gene            4777..5259
                     /locus_tag="PKI64_07785"
     CDS             4777..5259
                     /locus_tag="PKI64_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE00946.1"
                     /translation="MKKLLLSFFLLALLLAGARPATAQELQVFAARDFAAALKEIGAQ
                     FHGQTGFAVDFHWGDSTALAEKIIRTDRLPDVFFPASEAAMQLVVQKGLVDVALKRNI
                     LVVPAAVPATDETSPEMDYTSAAVLLDAANRLQAMAFLEFLVSEAAQTVFARYGFALP
                     "
     gene            complement(5238..6746)
                     /locus_tag="PKI64_07790"
     CDS             complement(5238..6746)
                     /locus_tag="PKI64_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00947.1"
                     /translation="MKQWVLVLAVAAAPWLAGLSFPFLYDDIGMIAENPFLEDPANVW
                     TVLSGRTLADPHVVNGRRPVVLATYFLDRAWHGLQPAGWRVTNLALHLACVALFMGLV
                     RRLTGHRFMAAAAGVLFGLHPVVSEVVHAPGFRADMLCLLLGLLALHGWLTPRARGVW
                     LGTVAAALALLAKETALALPLALALVLWLFPRDGLVRGAAARRVALAGGMAAAFFLLW
                     VWLPTELQAAGRVWNGESLRFPETIFSAPALWTRTLRLALVPWPLNVTPGFVPVASAF
                     SLRFGLGCGWLLLCGWGAWAARRVAPALSLALGWMLIFFLPVANLWPLLHPVADRYLY
                     PVLPGFALLVAWVLAQQTRRGRNVGLALLAVVYAGLSLVRVGQWQSAETLWTHAYFQN
                     QQSATAATWLGLLREDAADPAGARVWYQAAIAANPQAAPAWANWGMLEAKAGNLTTAE
                     PLLRRVTELQPDNPKGWRDWATCLAQMGRAEEAAAAHRVAAARAARQGSAKP"
     gene            6843..7700
                     /locus_tag="PKI64_07795"
     CDS             6843..7700
                     /locus_tag="PKI64_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOE00948.1"
                     /translation="MKQTHQPHRACLAAALATLLLLAPAAHAAKKWREYTDCHVIPHA
                     SNDGDSFHVKAGNIKTKTYLFRLYFVDTPESEKSLPERLQEQADYFGIANPLDVVQVG
                     KDAVKFTENFLADGKFTVYSRLADALGRSKMSRDYAMVLNSAGQDLGYELVRNGLARV
                     YGSSTDLSELDPYKKTADAWFRRLRQAELEAKNAKRGAWGVAAGPVSRLDALMAPRQV
                     DEQDVVLTRPTLLYPMDPANPRPLGTLQAGLTVHVIKGATPDKAHVRFTSSSGQVYEG
                     LARYQDLGL"
     gene            7707..8579
                     /locus_tag="PKI64_07800"
     CDS             7707..8579
                     /locus_tag="PKI64_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE00949.1"
                     /translation="MFDLPTYLAAGARWIEDALRRLVPPADTRPATLHAAIHHSLFAG
                     GKRLRPILCAAAAEACGASREVALFPAAAIECLHTYTLIHDDLPCMDNDDLRRGQPTC
                     HVVYGQAIALLAGDALQALAFELATRTPVATVEIIRELATAAGSTGVVGGQVEDIQGE
                     RLPPDADRLRYIQQHKTGDLIICACRMGMLAAGAPPTDLAAITDYAAAIGEAFQIADD
                     ILNATATPEQLGKAVGTDAAREKTTAVSVYGLAGARQRLHQLVATATAALAPLPGNTQ
                     PLAALAQHIATRQS"
     gene            8597..9901
                     /locus_tag="PKI64_07805"
     CDS             8597..9901
                     /locus_tag="PKI64_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00950.1"
                     /translation="MKKSCGLLVCAWLAVAAAGQDLAGYEWGEPKALAWGFNLTQQAV
                     IPDTVTNVIALAGGVYHSIALQADGRVAAWGWNKYGQTNVPPDLRDVVAIDAAGFYSL
                     ALRADGTVVTWGANYEERPIIMPPLTNIIAISAGFRHGLALTKDHTIINWGIPGFGPG
                     PPPPLTNVVAISAGDSHDLALCADGTVVAWGAEMPAGLQVPTNLSRVVAIAAGHYHNL
                     ALQADGTVVAWGWNNFGQTDVPPGLTNVIAIDAGTYHSLALQADGRVVAWGWNKYGQT
                     DVPSDLTNVALIAATEYHNLALRRPSEPTAETAQAAPSDAAARTTREPAALWRWGRND
                     GVSTGHAAQPTGGTTGEAAVEILAIAPAAGTALQSDRPGLEITWRSRAGHIYCVQVTD
                     DLGQAAWQTIQAGIPATPPQNAFTLATDERLRFYRIGEKLAE"
     gene            10321..10941
                     /locus_tag="PKI64_07810"
     CDS             10321..10941
                     /locus_tag="PKI64_07810"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOE00951.1"
                     /translation="MKKRIAVLLLGMAFVGPWAGAQQFNVIINNQWAMHYAEARNQPH
                     VVFSTSFQTQVGHGFVSPWLGQPPFCLVIGEERSFFNTQARIITPDDQKRNPPGTLLF
                     SLPRNLQAAAHVLQASGWRFYYSLGGYHVTLLIENIWMDPMQIVCCKGYMVVTADPDL
                     ALSSGPSSELTDWHVEQLERLAGLRDRGILTDDEFTREKERILNNR"
     gene            complement(11095..11487)
                     /locus_tag="PKI64_07815"
     CDS             complement(11095..11487)
                     /locus_tag="PKI64_07815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOE00952.1"
                     /translation="MSTSCPFCTLPADRIIATDGPCVAFRDGFPVSVGHTLIIPQRHV
                     ASFRDLAPDEWAAALRLAQRLAAELQQADPAIAGFNFGVNDGVTAGQSVFHCHFHLIP
                     RRAGDHPNPRGGVRGVIPHKADYPCPHR"
BASE COUNT         2139 a         3940 c         3511 g         2044 t
ORIGIN      
        1 acccacggta aaaatcacga gccaacagcc ccggcctcac cgaggccggc tacagaaaaa
       61 cctctgcacc ctcttctgtc cgctgtgcgc agcacgtggc cgtttcctga actctgaacc
      121 ctgacctctg ccgccgcacg tcccgcgcgc ggtcgggcgc ggcgcggggc taggtcatgc
      181 cgcgggcttt tttgatggcg ctgtaggcgg cgttgatgcg ctgaaacttt tcggtggcgg
      241 cacgttgaaa ctcagggccg agggtggcga ctttgtcggg gtggtgtttc atggagaggc
      301 ggcgataggc cttgcggatt tcgtcatcgg tggcatcggg ggctacttcc agcacgcggt
      361 agtcggcgta tttgtcgccg cgcggggcga acatggcggc gatggaatcg cggtcggcgg
      421 gacggatgcc cagagtgcgc gcgatgaggt cgagcaagcg ttcttcattc gggtgcaagt
      481 ggccgtcggc gtaggcaagc gaatagaggt agtgcaggag cgtgagccgc tgtgggttgc
      541 tcatgttggc gcggatttgg cggcaaacga tttcgacggg gatttcctcg cggagcagat
      601 cgcgcaggat gggcatgagg gtttgggcca gattccgccc gaattgttgg ataaagaatt
      661 tttgcgccac gataagttca gagcgcatgg cacggccatc ggcctgcatc atggcggcgg
      721 tgagggcgag caggctgacg gcaaaattgt ggcggtcggc gccggcggat gtgcggggcg
      781 ccgagcgtgg cggtcggggc gggggcttgg cggctttggt ggcattctcg gcgagggcgt
      841 tgatcagtgt gccggcggca tagccgatca gcccgccaag cggcccgcca aatgcccacc
      901 ccattgctcc ggtgacccac ggattaacca tcggatttct ccttggcggc aaccaactgc
      961 cgcaagcgtt tttcaatttc agccaagcgg tcgtccggat cgtgctggtg caggcggagg
     1021 tgccggccct tgaccatcag atattcatcg gcgccgaggc ccagcagcag gcggttgccg
     1081 tcggtggcga cgctcttcac gccgagggcc gcggccagca ggcggatgcg tgcgcaggcc
     1141 agcaggcggc gcacgggggc gggcaacggg ccgtagcggt cgcgaaactc ggcttgcagg
     1201 tcgcgcactt cgctggcgta ggccacgccg gcgagcttgc ggtagaggcc gatgcgctga
     1261 ctttcgtctt cgacatagtt gcgcgggatg acggcggctt gcgcctcgtg ggcggggtcg
     1321 ggcgtgaggt ccagaaaatc gaggtgcagt tgcacatcaa tcagcgggcg ttcgggagca
     1381 cctttcaggc gggcgatggt gcggttgagc aactggcagt agagatcaaa tcccacggcg
     1441 gcaatgtggc cgctttgcgc cgcgccgagg acattgccgg cgccgcggat ttcgagatcg
     1501 cgcagggcga gcttgaaacc ggcgccgagg ctggtgtggc ggcggatggc gctgatgcgc
     1561 cggcgggcgg cgttgaaaag cgcgccatgg cgcggcagca gcagataggc ataggcctgg
     1621 tgccgactgc gccccacgcg cccgcgcaac tgatacagat cggccatgcc gaagcggtcg
     1681 gcgcggtcaa tcaggatggt gttgacgttg gggatatcca cgccgctctc gatgatggtg
     1741 gtgcagagaa gcacatcgaa ttcgcgccgc acaaaccggc gcatgatgtc agcgagctgg
     1801 cgctccttca tttggccgtg gcccacgccc acgcggatgc ccggcagcag gcgcgccaac
     1861 cgctcgcgca tccactcaat ggtgcgcacg cggttatgga gcaggaagac ctgcccgccg
     1921 cggttgacct cgcgcaggat gacctcgcgc aggagttcgt cggagtcttg ggcgatgtag
     1981 gtttcgatgg cgaggcgctc gcgcgggggg gtctggatga ggctcatgtc gcgcgcgccg
     2041 gtgaggctca ggtagagcgt gcgcgggatg ggcgtggcag agagagttag cacatccacc
     2101 agctcgcgca gacgcttgag gtgctccttg gcggccacgc cgaaccgttg ttcttcatca
     2161 atgatcacga gcccgagatc ggcaaagcgg acatcgcgtt gcagcaggcg gtgggtgccg
     2221 atgagcacat ccactttttt ttcggctaac cggacgagcg tggccgcctg ttccgcgcgc
     2281 gaccggaagc ggctgaccat ttccaccgtg atgggaaagc gccccatgcg ctcctgaaaa
     2341 gtttcgtaat gctgctgggc cagcacggtg gtcggcacca gcacggccac ctgcttgcca
     2401 tccatcacgg ccttgaacgc ggcgcgcagg gccacttcgg ttttgccgta gccgacgtcg
     2461 ccgcaaatca ggcggtccat cggctggggg gcttccatgt cgcgcttgac gtcggcgatg
     2521 gcgcgcaact ggtcgtcggt ttctggaaag ggaaaggcct gctcaaaatc gtgctgccat
     2581 ggcgtatcgg gcgcgaaggc gtggccttgc agcgcggccc ggcgcgcctg ggtttccagc
     2641 atttgcgcgg cgagatcgtt cgcggcgcgc gcggcagcga ttttttcttt ggtccagcgc
     2701 ttgccgccca gcgtgtgcag cggcggcttg gtgcgcccca ccccgatata gcggctgagc
     2761 aggtgcgtct ggctggtggg cacatagagc cgcgcctcgt cggcatattc cacggcaagg
     2821 gcttcttgca gttggccatc gaagacaatt tcatagaggc caagatattt gccgatgccg
     2881 tgctccacgt gcacgaccaa ttcgcccgga cgcagatcgc tccagtcggt caaccgttcg
     2941 ccggtggcgt cgcgcgcggc gccgccgcgc ggtttgcgac ggggggggag cgcgcggcgc
     3001 gcaccgtaca aatcggcttc ggccacggcc atccactggg cagccggcac ttcgcaaccg
     3061 ccactcaagc cgcccagccg caggtgaatg cccttggttt tgcggaacga cgcagcaaaa
     3121 cgccgccgcc ccgcatccgt cgcgaaaaag atatccaccg tccagccggc ggcggctcgt
     3181 tcgtgcaact gcgcgaacca tttttggcgc tcggtttcga ggacatccgg cggcacggct
     3241 tgcgccgtca gcgcggccaa accggccacg ggatgcgcgc cggagtcgtg cgccaggtcc
     3301 gcgcccccgg ccgtcaactg cccgccgccc gcgcagcggc gggccagcat cagttgccag
     3361 ttggcataat cgcgttcggc ggccaacgcg gtttcgccgc tgccggccaa ctccgcgctc
     3421 atggcgtagt ggtgggtgat ggcctcattg tccagccaca gaaacgaggc atccgcaggc
     3481 acataatccg tgggcgcggc ggcggggcca tccgcggcgg cgtcgcgcgc gggtggcagc
     3541 catacttcgc gcaatttgtc gcgcgagcgt tgctccgccg gatcaaacga gcggaggcct
     3601 tcgagcacat cgccaaaaaa ctcgagacgt acgggccagt cttccgtcag cggccacaca
     3661 tccagcaaac caccacgacg cgaagcctgc cctttggcga ggacttcgct ctcgaaggcg
     3721 taacctgcgg caaccagctt gccgcagagt tgttcaagat cgcactcctg ttgttcgcgc
     3781 aggtggaggg tatcctgctg caactgctcc ggcgtggcca ccggttccag cagtgcttgt
     3841 acgcacgtca ccagcagccc ccgcccctgc caggccaagc attgccgcag ggtattcatc
     3901 cgctcgccgc tgacatcggg cgaaggctga ccggtaatca gcttggaagc ctccacattc
     3961 agcgcgggca gcttgaccat gggcagaccg ccaccgagcg tttgcacgtc ctgccatagt
     4021 aattccagcg ccatggtgtt ttcggccacc accaccagca ttcccggcgc cacctgcgcc
     4081 gcgcccagcg ccagccaggc gcgcgccgag tcatcgagaa tggccagctc gccatgcccg
     4141 cccggcgcca gcgcccgcgc cacatagtcc cgcgtggccg ccagcagcgg cggcacattc
     4201 agcggttgct cacgcgccgc catggcatcc ccgtgcgctt ccggtagcaa ccagccgcat
     4261 gtgccgctgc gatgggtttg gtaatgtggc cgcctgaaac atatccgcct actataacaa
     4321 cccacgctcg ctttacgcaa aaaatcggtt cctcgctgtt ttcagtggct gaatcgtcct
     4381 gtttgagacg tggaagcggc tgtcatcggg ctaatttctt tttatcattt taactacact
     4441 gtccagagtt tataaagtct ggacaaatgt tgtagtattc atatctcaat cacatacaac
     4501 ttgttatgtt taattttctg actattttta atcggtcatt tttagggaaa tgtccacagt
     4561 ttataaagtg tggacataat taaggtgagt gatttttgtg tttttatccg cgcggcgcgg
     4621 ttccgcatga cagttgctcc ccacaaccgg cactcaaatc ggcgacgagc aaaaaacggc
     4681 gtggcccgcc gcccgatttg gcgtggccat gtccggcatt gacaaaccaa ccccgctttt
     4741 cttgaatgcc gcgcaattca atttctacgg acatccatga aaaaactgtt gttatccttt
     4801 tttctgctgg ccttgctgct tgccggcgcg cgccccgcca ccgcgcagga gttgcaggtg
     4861 ttcgccgcgc gtgatttcgc ggccgcgctc aaggaaatcg gcgcgcagtt tcacgggcaa
     4921 accggttttg cggtagattt tcactggggc gattccaccg cgttggcaga aaaaatcatc
     4981 cgcaccgacc ggctaccgga cgtctttttt ccggcaagcg aagccgccat gcagctcgtg
     5041 gtgcaaaagg gtctggtgga tgtggcgcta aaacgcaaca ttctcgtggt gcccgccgcc
     5101 gtaccggcca ccgatgaaac ctcgccggaa atggactata cctccgccgc cgtgctgttg
     5161 gatgccgcca accggctgca agccatggcg tttttggagt ttctggtctc ggaagccgcg
     5221 cagacggtgt ttgcccgtta cggcttcgcg ctgccctgac gcgccgcccg cgccgcggcc
     5281 acgcggtgcg ccgccgcggc ttcctcggcg cggcccattt gcgcgaggca cgtcgcccaa
     5341 tcgcgccagc ctttcgggtt gtcgggctgg agttccgtca cgcggcgcag cagcggctcg
     5401 gccgtcgtca gattgccggc cttggcctcc agcattcccc agttagccca cgcgggcgcg
     5461 gcctgcggat tggccgcgat ggcggcctga taccacaccc gcgcaccggc ggggtcggcg
     5521 gcatcttcgc gcagcagacc cagccaggtg gcggccgtcg cgctttgctg attttgaaaa
     5581 taggcgtggg tccagagcgt ttcggctgat tgccactgcc ccacgcgcac caaggaaagc
     5641 cccgcataga ccaccgccag cagggccagc cccacgttac gcccgcgccg ggtttgctgc
     5701 gccagcaccc acgccaccag cagggcgaag cccggcagta cgggatatag atagcggtcg
     5761 gccaccggat gcagcagcgg ccagaggttc gccaccggca gaaagaaaat cagcatccac
     5821 cccaacgcca gcgagagtgc cggcgccact cgccgcgcgg cccacgcgcc ccagccgcac
     5881 agcagtagcc agccgcaccc cagcccgaac cgcagcgaga acgccgacgc caccgggacg
     5941 aatcccggcg tgacgttcaa cggccaaggc accagcgcca gccggagcgt acgcgtccaa
     6001 agcgccggcg cggaaaaaat ggtttccgga aagcgcagcg attctccatt ccacacccgc
     6061 cccgccgctt gcagctcggt gggcagccaa acccacaaca gaaaaaaggc tgccgccatg
     6121 ccgcccgcca gcgcgactcg ccgcgcggca gcgccgcgca ccagcccatc gcgcgggaac
     6181 agccacagca ccagtgccag cgccagcggc aacgccagcg cggtttcctt ggccagcagc
     6241 gccaacgcgg cggcgaccgt gcccaaccac actccgcgcg cgcggggtgt cagccatccg
     6301 tgcagcgcca acagccccag cagcaaacac agcatatccg cgcggaagcc cggcgcgtga
     6361 accacctccg acaccaccgg atgcaggccg aatagaaccc ccgccgccgc ggccatgaag
     6421 cggtggcccg tcagccgccg caccagcccc atgaacagcg ccacgcaggc caaatgcagc
     6481 gccagattcg tcacgcgcca gcccgccggc tgcaacccat gccacgcccg atccagaaaa
     6541 taggtcgcca gcaccactgg gcggcgtccg ttcactacgt gcgggtctgc cagcgtccgc
     6601 ccgctcaaca ccgtccacac gttggctggg tcttccagaa acggattttc ggcaatcatg
     6661 ccaatgtcgt catataaaaa cggaaacgac aaccccgcca gccacggtgc ggccgccacc
     6721 gccagcacca acacccattg cttcatccgc cacatgcccg cattgaagtc gcagcgcccc
     6781 cgctttgtca acgccccctt ttttgctttt cctcacgcgc caaatgtccc aaggtatctg
     6841 gcatgaagca aacgcaccag ccccaccggg cctgccttgc ggcggccctc gcgactctgc
     6901 tcctgctggc tcccgccgcc cacgctgcca aaaaatggcg cgaatatacg gactgccatg
     6961 tcatcccgca cgcctccaac gatggcgaca gtttccacgt caaggccggc aacatcaaaa
     7021 ccaagaccta cctcttccgg ctgtacttcg tagacacccc cgaatcggaa aaatccctgc
     7081 ccgagcgctt gcaggagcag gccgattatt tcggcatagc caatcctctc gatgtggtgc
     7141 aggtcggcaa ggacgccgtc aagttcaccg aaaacttttt ggccgatggc aaattcaccg
     7201 tctattcccg tttggccgac gccctcggcc gttccaaaat gagccgcgac tacgccatgg
     7261 ttctcaactc cgccggccag gacctcggct atgaactcgt gcgcaacggc ctcgcccgcg
     7321 tctatggcag cagcaccgac ctctcggaac tcgatccgta taaaaagacg gccgacgcct
     7381 ggttccgccg cctgcggcaa gccgaattgg aagccaaaaa cgccaagcgc ggcgcgtggg
     7441 gcgtcgccgc cggcccggtc tcgcgccttg atgccctcat ggccccgcgc caggtggatg
     7501 agcaggatgt ggtccttacc cgccccaccc tgctctaccc gatggaccct gccaaccccc
     7561 gtcccctcgg cacgctgcag gccggcctga ccgtccacgt gattaaaggc gccacgcccg
     7621 acaaagccca cgtccgcttc acctcatcct ctggccaagt ctatgaaggc ttggcccgct
     7681 accaggacct cggcctctga accgccatgt tcgacctgcc aacctatttg gccgctggcg
     7741 cacgttggat tgaggacgcg ctgcgccgcc tcgtccctcc cgccgatacc cgccccgcca
     7801 ccctgcatgc ggccatccac cacagcctct tcgccggcgg caagcggctg cgccccatcc
     7861 tctgcgccgc cgccgccgaa gcctgcggcg cgtcgcgcga ggttgccctc ttccccgccg
     7921 ccgccatcga gtgcctccac acctacaccc tcatccacga cgacctcccc tgcatggaca
     7981 acgacgacct gcgccgcggc cagcccacct gccatgtggt gtatggccag gccatcgccc
     8041 tcctcgctgg cgatgccctc caagccctcg cctttgaact cgccacgcgc acacccgtcg
     8101 ccaccgtgga aattatccgt gaactcgcca ccgccgccgg ctccaccggc gtagttggcg
     8161 ggcaggtgga agacatccaa ggcgaacgcc tcccccccga tgccgaccgc ctgcgctaca
     8221 tccagcaaca caaaaccggc gacctgatca tctgtgcctg ccgcatgggc atgctggccg
     8281 ccggcgcccc ccccaccgac cttgccgcca tcaccgacta cgccgccgcc atcggcgaag
     8341 cctttcaaat tgccgacgac attctcaacg ccaccgccac ccccgagcaa cttggcaaag
     8401 ccgtcggcac cgatgccgcg cgcgaaaaaa ccaccgctgt ctcggtctat ggcctcgccg
     8461 gtgcgcgcca acgcctccat caactcgtcg ccaccgccac tgccgccctc gcccccctcc
     8521 ccggcaacac ccaacccctc gccgccctcg cccaacacat cgccacccgc caatcctgaa
     8581 cgacccgcgg aacaaaatga aaaaaagttg cggcctcttg gtgtgcgcgt ggctggccgt
     8641 ggccgcggca gggcaagacc tggccggcta cgaatggggt gaaccgaagg cgctggcgtg
     8701 gggcttcaac ctaacccagc aggccgtcat ccccgacacc gtcacaaacg tcattgcgct
     8761 ggccggcggc gtctaccaca gcatcgcgct gcaggccgat ggccgggtcg cggcgtgggg
     8821 ctggaacaag tatggccaaa ccaatgtgcc gcccgacctg cgggatgtgg tggccattga
     8881 tgccgccggg ttttacagcc ttgctctgcg cgccgatggc accgtcgtca cctggggcgc
     8941 gaactacgaa gaacgaccga tcatcatgcc gccactgacg aatatcatcg ccatctccgc
     9001 gggctttcgc cacggcctcg ccctcaccaa agaccacacc atcatcaact ggggcatccc
     9061 cggctttggc cccggcccgc cgccaccact caccaatgtg gtggcgattt cggcgggcga
     9121 tagccatgac ctcgccttgt gcgcggatgg caccgttgtc gcgtggggcg cggagatgcc
     9181 ggcggggctc caggtgccca ccaacctctc gcgcgtggta gccatcgcgg ccggtcatta
     9241 ccacaatctc gccttgcagg ccgatggcac cgtggtggcc tggggctgga ataacttcgg
     9301 ccaaaccgat gtgccccccg gcttgaccaa cgtcatcgcc attgatgccg gcacatatca
     9361 cagcctcgcc ctgcaagccg atggccgggt cgtggcgtgg ggctggaaca agtatggcca
     9421 aaccgatgtg ccctccgatc tgactaacgt ggccctgatt gccgccaccg aataccataa
     9481 tctggccttg cgccgcccgt cggaacccac cgcggaaact gcgcaagccg cgccctccga
     9541 cgccgcggca cgcaccaccc gcgaacctgc ggcactgtgg cgctggggtc gtaacgatgg
     9601 cgtcagcacg ggccacgccg cccaacccac cgggggtacc accggcgaag ccgcagtgga
     9661 aatcctcgcc atcgccccgg ccgccggcac cgccctgcaa agcgaccgcc ccggccttga
     9721 gattacgtgg cgtagtcgcg ccggccatat ttattgcgtg caagtcaccg acgacctcgg
     9781 gcaagcggca tggcaaacca tccaagccgg catccccgcc accccgccgc aaaacgcctt
     9841 cacgctcgcc actgacgaac gcctgcgctt ttaccgcatc ggcgaaaaac ttgccgaata
     9901 aattctcacc ccccaccgca cttttccaca ctgtggaaaa ttattttcca cactatggaa
     9961 aactccacgg cgaaagttcc acaccgtgga aaaattcacg ttctgccgcc ccattttttc
    10021 cacacaatgg aacaactttg agtccacttt tccacacaat ggaacaactt tgagcaaaaa
    10081 gttccacact gtggaaaaaa attttttttg aacccgccgc cggtacaacg gcggcctaca
    10141 ttaaacccgg cctcagcgag gccggctaca gatgaaccca ttcctcaaaa ccccatgcat
    10201 tctctccctt tacgcctctc gcgtcgctta cgttgcacgt cttcggctgt tttcgcccgg
    10261 taacctaatc ggcaccgccc gcgactcttt tcgtaacgag caaggcgaaa gggtgtgaga
    10321 atgaaaaagc ggattgcggt tcttttgttg ggaatggcgt tcgtcggacc atgggcgggc
    10381 gcgcagcaat tcaatgtgat catcaacaac caatgggcca tgcattacgc ggaggctcgg
    10441 aatcagccgc atgtggtctt cagcacctca ttccaaacgc aggtgggcca cggattcgtt
    10501 agcccgtggc tgggccagcc ccctttttgc ctggtcatcg gcgaggaacg ctcctttttc
    10561 aatacgcagg cacggattat tacccccgat gaccagaaac gaaatccgcc cggcacgttg
    10621 ctgttttccc tgccgcgcaa tctgcaggcc gccgcccatg ttttgcaggc cagcggatgg
    10681 cgtttctatt actcccttgg cggctatcac gtcaccctgc tcatagaaaa catttggatg
    10741 gacccgatgc agattgtctg ttgcaagggc tacatggttg tgaccgcaga cccggacctt
    10801 gctctgtcct ccggcccaag ttcggagctg accgattggc atgtcgagca attggagcgt
    10861 ctggccggct tgcgcgaccg cggcatcctg accgatgatg aatttacccg ggaaaaggaa
    10921 cgcattttga acaaccgctg atccagtcca gcttgcaatt tccggtacga tgtttcaacc
    10981 ggcctcggcg aggccggcta caggcgaacc ctttccccaa ccccaaccat tcactccctt
    11041 ttccgcgctc tacgcttgcg gccttttgcg tctctcgcgg cgtttgcgtt aatgtcagcg
    11101 gtgggggcag gggtaatcgg ctttgtgggg gatgacgccg cggacgccgc cgcggggatt
    11161 ggggtggtcg cccgcgcggc gggggatcaa atgaaaatgg caatggaaga ccgattgccc
    11221 ggctgtgacg ccatcgttga cgccaaaatt gaagccggcg atggcggggt cggcctgctg
    11281 caattccgcg gcgagtcgtt gcgcgaggcg caaggcggcg gcccattcgt ctggcgcgag
    11341 atcgcggaac gacgccacgt ggcgttgcgg gatgatcagc gtatggccga cgctgacggg
    11401 gaatccatcg cgaaaggcca cacaggggcc atcggtggca atgatccggt cggccggcag
    11461 cgtgcagaat gggcaggacg tactcatggc gcggagcata aaacccgtgc acgcgccgcg
    11521 caaatgaaaa gcgcgggaac gccattttgc ggatggcccg cgcgcggcgg gcggcggctt
    11581 gggcggtggg gtgcacgaca taaaagtcgg ttattaagcc gcgcgatcat ccca
//
LOCUS       DAOPNP010000079        11632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6392_length_11632_cov_7.614753, whole genome shotgun sequence.
ACCESSION   DAOPNP010000079 DAOPNP010000000
VERSION     DAOPNP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_11632_cov_7.614753"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKI64_07820"
     CDS             complement(<1..343)
                     /locus_tag="PKI64_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00953.1"
                     /translation="MRVTHPICIFGLLLTVTLGAGCRSFDAARVRQEQTDTFTSNLAA
                     RAEAELAQPLSLADCVRIAMSNNYAVRKADLDQELYRIGKNVAFTAFLPNVAAQAGYT
                     SFAKDPKITERE"
     gene            complement(362..832)
                     /locus_tag="PKI64_07825"
     CDS             complement(362..832)
                     /locus_tag="PKI64_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOE00954.1"
                     /translation="MKTKRTSTAGHARPQPAQSLLPLWLRMGREYNCRLQQWQLPINL
                     TIPLLKLHLHADISEPAALAEASSLPRQTMTFVLDALEKRELAYRTPHPNDRRRKIIQ
                     LSPKGRKLATALYKELLAFEAAALQSLGETAAPMLHQLVARFTDALAAENRRHA"
     gene            1128..2207
                     /locus_tag="PKI64_07830"
     CDS             1128..2207
                     /locus_tag="PKI64_07830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE00955.1"
                     /translation="MKTFNHRTDPLQETRTPFLSVVIPTMGRDILIRTLESLAAAEGF
                     AAIEIIVAGRVPDATVAAQLRDLCARHANIRHLDIQFATGDSSRKKNEGAAMARAPLI
                     AFLDDDVIVATDWPRRLAEPFQDPQVGLLSGPSLVPEDINWVGRLAGLALSSPAAAYV
                     AERYRPNRDAPYAVDWDRIIGCNAAYRREAFEQMGGFPVDFYPGEEMIAAFRAEQAGW
                     KLMFVPAAWVKHYPRQSLRRFWRQVWGYGATRIRLLRAGVSFNPLSLVPAAWVTGTAV
                     LVALSFVWRWAGWLLALELGLYGLFALWVTGGTVRQTRRWADLGLLVVIPLMHLAYGL
                     AEWAEFLRPGKDFSETVAPAKNQSK"
     gene            2402..3838
                     /locus_tag="PKI64_07835"
     CDS             2402..3838
                     /locus_tag="PKI64_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00956.1"
                     /translation="MAKEKLDEQVKPLETTPPPAAVVDDADETGGFSSTRPTTDANVW
                     IQIVGAVICTMATCYFLYFWGHNAELGSWPSYLNALVNQRGPVQWLELLCFYVIIFFL
                     FQKNRILRNQIKHIAADSAKVRGVNMESEEEIQALRRRIQETGGDKESVLLSRLNRAL
                     GLWVSTKDLGRVTGWIGTEAGRDSTISDMTFTLVRLMMWVIPILGFIGTVQGLGAAVG
                     GFANFLSGSAELSAIKGAIANVTISLGVAFDTTFLALMLVTVVQFPLTSLMRRESTFM
                     AEVDIYLDEHFIRRLPSAEQQPVVIENLEDSIEAAFRRYIPDPDRYEEVFTVSIEKAG
                     DELKRQFEELTRRYVEVRQQATDDEVKALAAAMEHAHKRAAEMADRYARSADSIQATL
                     GESLQKAANAAGVVEQQITTIMDLGAKIQELLKVEQALERAMAGIAGSEEFRLAFSQL
                     REHLATTDEFCRRLSKPRVITLHEEVIS"
     gene            3931..5811
                     /locus_tag="PKI64_07840"
     CDS             3931..5811
                     /locus_tag="PKI64_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00957.1"
                     /translation="MASGQTLTGRVLASGLQEPTGTAVHPISGDVYVSEQATGRILVL
                     KNGKAESAMAADWGVSNVLPRWAISAEMPTEKWMEAKLNKPGAIAFTTNGTLFVTEQI
                     PNGRILELYPDEHGLFTTAVGVPVPWLDQEFQWRNIFVDPIGRVFIAGADEIGSDFMK
                     FGSVLMREANGNWWVIDFGPFANFTALALSERLDVLLVGDRTKGNLSWWEVDRHMMLG
                     GAPDVLGRADMAALAVYPDGTFIIAAQEGTGKATLRRFEPFTGQQQTLASDLTSVGAI
                     AMDRPRARYIVTDPAAGKVVEYTPPAGLKFNESVMRQIVRSVEGMVGMPSEAPAFLNN
                     FFERLQDAAKEILPDDSTHAVDFNLSDIAGKMPVVAGRVRATVEVEGAEDDPLQSVEF
                     FLLFPSKVVLTESAVSPSLSFFSARRKSGKLEQTRPVFQGNVGVYRMSGTNYSQVASA
                     QGGLHIPIVVCGMDQADGGVYVNLSFLGAGIYGDYYLTLFQGPREQKAKLVVKSVEAA
                     SGNVTYEASFMEDAVIEGMQGSTISKEQYSNLLISGFGGGAGANRSVGWLKLGQFPAQ
                     MTIAFGDSAMDTEVTSAQSGLKEIVEKKRVELSLETTEDIGTAEEQHPAAPDNKESAP
                     WP"
     gene            5802..6464
                     /locus_tag="PKI64_07845"
     CDS             5802..6464
                     /locus_tag="PKI64_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00958.1"
                     /translation="MAMKMAEFDQSGFQNIVLMLIGLMTLVMVSNVLTIISNPDNIKI
                     GAVVTGSVYGEEEDAETFIPPKFQNMKQDPIYLDVEPTRLTIYPELKVIEERDLVFEG
                     NDFEQFLDDVEKVRSRRYIVLLLRPGSAQFQRKLRKVIRDRGIDVGFEPWEAGREIQV
                     VRAWEELVPEGEGGEGEAVEGTEAGATETTPAGEPAAGEESMTEAPAATETMPEPTGE
                     GM"
     gene            6468..7094
                     /locus_tag="PKI64_07850"
     CDS             6468..7094
                     /locus_tag="PKI64_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00959.1"
                     /translation="MSEGPSIDLSSLTDIMTCILGCLVFIIMLTGIDASQITVLVATP
                     KEMTADDKSPVFFECRKDQLFEISLDQLKKACDEKTEEIRDWVKGNEQEFLKQAAQTM
                     LEVNGQRLDYTYALMGKYVLMPIMDATGYALGTATEETPDKWYASRLAQIDPETQFIC
                     FLVRPDSYRVFQRARNLAWINNINVACELQDEKNPISIGPGGERMYMQ"
     gene            7116..7460
                     /locus_tag="PKI64_07855"
     CDS             7116..7460
                     /locus_tag="PKI64_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOE00960.1"
                     /translation="MPESFFQCQHCGACCRWPGSVLLTAADVAKLARHLNMTEGEFIQ
                     QHTTLARNRAQLTLKEQADGACAFLTADNRCAVYPARPKQCRDFPHGWTVAGCPAAQE
                     PAVTKPAATAPG"
     gene            complement(7429..7890)
                     /locus_tag="PKI64_07860"
     CDS             complement(7429..7890)
                     /locus_tag="PKI64_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 domain-containing protein"
                     /protein_id="HOE00961.1"
                     /translation="METLAMIVVRRGESPRKCTVRPLRGTPGLIFMSYPLRQRPNLAG
                     YLLLAPDAPPLTPADAGSPLLLLDASWHHAAAMRRAVEPIAARSIPPGWQTAYPRTSR
                     AHSDPPAGLATVEALFAALCTLGRRDDSLLRHYQWRKQFLTLNQALLPPVL"
     gene            7991..9592
                     /locus_tag="PKI64_07865"
     CDS             7991..9592
                     /locus_tag="PKI64_07865"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOE00962.1"
                     /translation="MKVALLQMNPTVGALRENAARILKLAHAAAEAGAEIAVFPEMAL
                     CGYSPGDLVQRARFLDDVAATMAQLGAELPAGLLAIIGAPWRKDGQVINAALLWRDGQ
                     LLAVAGKTVLDDSVEFAESQVFVPASATLVHEVKGVRVGVGIGRSTPGSGEVVIQLAA
                     QPYRHERVAALTERASQLAQATGVPVLVANLTGSHEGLVFAGGNVAVAADGTVATRAT
                     LLREEMLLVNLVRKNGGWQPVAGAVVPLPSAVAGTYAALQTGLRDYVEKNKFPGAMVA
                     LSGGMDSALVAAIAVDALGPNRVFGVTMPSPVTANETLADALGLAQRLNIPCLKIPLA
                     PAMGTLQDLLDRADLQTYWAAPLPGSTAAENVQARLRGLLAMALSNRYGHLLVCASNK
                     SEMAVGYATLYGDMAGGLALLKDVPKTLVYELAHWRNRQGEVIPPGTIARPPSAELKA
                     NQRDTDSLPAYDELDPILERLMDRQQDVAEIVRAGFAEATVRRVARMVARAEFKRRQS
                     APGIWVTSPQRRWSASRPLTNGYAE"
     gene            complement(9580..10887)
                     /locus_tag="PKI64_07870"
     CDS             complement(9580..10887)
                     /locus_tag="PKI64_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE00963.1"
                     /translation="MPRTTNLSLPVIMSSVLLAVLLAFGLICWWLLPVATLAAHFGAF
                     VGLAAVLAGLLVASLVRAWRTPALPPLPPQRANPILHATGREDFDALLDLFQDGTLTV
                     EENDHILRANTAAEKLLAPTGPGLAGRHLADLADGAHLLALLTAIRTSAAFQAVDLRL
                     TASNSLCNVAGVPLLHEHAGKPGQVLLVLRDISRITQLEQAGEEYAVNVSHELKTPLT
                     LILGYTETLLADPNVTPEFRTQSLRTIERHSRRILRIVDDLLRLAWLQSERDAIGGVP
                     ITPTIVADVVAEAVSVCAEWAQQADIAIQTDIPVGLVWGLHANLMTVALANLIKNAVL
                     YALVGPVEIRAQVLPSGNLELSVKDRGPGLKPEAAQHIFDRFYRTDKGRSRAAGGSGL
                     GLPIVQQILLAHHGTARVETAPGAGCNFILELPSGRAASDYSA"
     gene            complement(10892..11569)
                     /locus_tag="PKI64_07875"
     CDS             complement(10892..11569)
                     /locus_tag="PKI64_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE00964.1"
                     /translation="MIVEDEPDIQELLKFHLEKHEYAPVVAGTGEEALAILKSRSIVL
                     VLLDLMLPGMDGLAVCSKMKQHRKLRAIPIIMLTAKNSEKDIITGLEIGADDYVTKPF
                     NPSVLMARVQAILRRPGIAKPDADETPPIKYGPLVIDPGRHQVTVKGREVHLTYTEFR
                     ILQLLAQNPGKVFSRSQIVEYVRGEAQTVTERIVDVQMVSLRKKLNLRDDLIETVRGV
                     GYRGKEV"
BASE COUNT         2284 a         3319 c         3755 g         2274 t
ORIGIN      
        1 actcgcgctc ggtgattttg gggtccttgg caaaggatgt gtagcccgct tgcgcggcca
       61 cgttgggcag gaatgccgtg aaagccacat ttttgccgat gcggtacagc tcttgatcga
      121 ggtcggcctt gcgcacagca tagttgttgc tcatggcgat gcggacgcaa tcggccagcg
      181 acagcggctg ggccagttca gcctctgcgc gcgcggccaa gttcgaggtg aacgtatcgg
      241 tctgctcctg ccggacgcgt gcggcatcaa agctgcggca gccggcgcca agcgtcacgg
      301 tcagcaggag gccgaagata cagatggggt gggtgacacg catgagaatt atcctttcgg
      361 tttaggcgtg gcggcgattt tctgcggcca gcgcgtcggt gaaccgggcc accagttggt
      421 ggagcatggg cgcggcggtt tcgcccagcg attgcaacgc ggccgcctcg aaggccagca
      481 gttccttata caaggccgtg gccagcttgc ggcccttggg ggaaagctga atgattttgc
      541 gccggcggtc gttggggtgg ggggtgcgat aagccagttc ccgtttttcg agcgcgtcga
      601 gcacaaaggt catggtctgg cgcgggagac tgctggcttc ggccagcgcg gcaggttcgg
      661 aaatatccgc gtgcaggtgc agcttcagca gggggatggt caaattgatc ggcaactgcc
      721 actgctggag gcggcaatta tactcgcgcc ccatgcgcag ccagagcggg aggaggcttt
      781 gcgccggttg cggccgcgcg tggccagccg tcgaagttcg tttggttttc atcggttctg
      841 ctctcatttc gtatgattcg atacgggata atacaatgtg ggattaaaat gtcaagaggc
      901 cgggcaaaat atttttttta cattgccagc aggggcggga atgactttga tgggccgcat
      961 gaaatccgtg ctcatgtccc cggctgtgcc ggtctacgcg ctctgcggca aacccagcca
     1021 agaggcgccc acaatggtgc ggcgttacgc acggtcgcca atgccatgct gcaggcagga
     1081 cgctgatttg ccggtgggcg tctggtggcg cgacgcatgc ggcggagttg aaaacattca
     1141 atcacaggac tgaccccttg caggagacgc gcacgccgtt tttgtccgtt gtgatcccga
     1201 cgatgggtcg ggacattctg attcgtacgc tggaatcgtt ggcggcggcg gaggggttcg
     1261 cggcgattga aatcatcgtg gccgggcggg tgccggatgc gacggttgcc gcccagttgc
     1321 gcgatttgtg cgcgcgccat gccaacatcc gccacttgga catccaattt gccaccggcg
     1381 actccagccg caagaaaaac gaaggcgccg ccatggcgcg agcgccgctg attgcgtttt
     1441 tggatgatga tgtgattgtg gccaccgatt ggccgcggcg cttggcggaa ccgtttcaag
     1501 acccgcaagt cggtctgctc agcggcccca gcctggtgcc ggaggatatc aattgggtgg
     1561 ggcggctggc ggggctggcg ctgtcgtcgc cggcggcggc ttatgtggcc gaacgctatc
     1621 ggcccaaccg cgatgcgccc tacgcggtgg actgggaccg gatcattggc tgcaacgcag
     1681 cctatcggcg cgaggcgttt gagcaaatgg ggggcttccc ggtggatttt tatcccggtg
     1741 aagaaatgat cgcggctttc cgcgcggagc aagccggctg gaagctcatg tttgtgccgg
     1801 cagcttgggt gaagcactat ccgcggcaat cgctacgccg cttctggcgg caggtgtggg
     1861 gctatggcgc cacgcgcatc cggttgctgc gggccggggt atcattcaac ccactctcgc
     1921 tggtgccggc cgcgtgggtg accggcacgg cagttctggt ggcgttaagc tttgtttggc
     1981 gctgggcggg gtggctgctg gcgctggaac tcgggctcta cggtcttttt gccctgtggg
     2041 tgacgggcgg cacggtgcgg caaacccgcc ggtgggccga cctcggtttg ctggtcgtga
     2101 ttcccctgat gcacctggcc tatgggttgg ccgaatgggc ggaatttttg cggccgggaa
     2161 aagattttag tgaaacggtg gcaccagcca aaaaccagag caagtaattt tgccggcgac
     2221 ggttgcatga acagtctggc cgatgtcccc gattggggcg gcgcagagat attcgccgcc
     2281 gggaaatatt caggtgcggt taatttgtta tcgacggcag ggcaaagttt tgggatagta
     2341 tctcgaaaaa tcaggccgca tgtgcggcga ggccaagcca cccgtaagca ggttatagat
     2401 tatggcgaaa gaaaaactcg acgaacaggt taagcccttg gaaaccacgc ccccccccgc
     2461 cgcggttgtg gacgacgcag acgaaaccgg ggggtttagc agtacccgcc ccactacgga
     2521 tgccaacgtc tggatccaga ttgttggcgc ggtcatctgc accatggcca cctgctattt
     2581 cctgtatttt tggggtcata acgccgaatt aggctcttgg cccagctacc tgaacgcgct
     2641 ggtgaaccaa cgcgggccgg ttcaatggct cgaattgctt tgcttctacg tcattatttt
     2701 cttcctgttc cagaagaacc ggattctgcg caaccagatc aagcatatcg cggctgactc
     2761 cgccaaggtc cgcggcgtga acatggaaag cgaagaagaa atccaggccc tgcgccggcg
     2821 cattcaggaa accggcggcg acaaggaaag cgtgctgttg agccgcttga accgcgccct
     2881 cggcttgtgg gtgtccacga aggacttggg ccgcgtgacg ggctggatcg gcacggaagc
     2941 cggccgcgac tccacgattt cggacatgac cttcaccctc gtgcgtctga tgatgtgggt
     3001 gattcccatt ctcgggttca tcggtacggt gcagggactg ggcgcggccg tcggcggatt
     3061 cgccaacttc ttgtcgggct cggcggaact gtcggccatc aagggcgcca tcgccaacgt
     3121 gaccatcagc ctgggtgtgg cgttcgacac cacattcctt gcgctgatgc tggtgacggt
     3181 ggtgcaattc ccgctgacct cgctgatgcg gcgtgaatcc acattcatgg cggaagtgga
     3241 tatctatttg gacgaacact tcatccgccg tctgccttcg gccgagcagc agcccgtggt
     3301 cattgaaaac ctggaagact ccatcgaagc ggccttccgc cgttacattc cggacccgga
     3361 tcgctacgaa gaagtcttca ccgtgtccat cgaaaaggca ggcgatgaac tcaagcgtca
     3421 atttgaagag ctaacccgcc gctatgtcga ggtgcgccag caggccacag acgacgaagt
     3481 caaggcgctg gccgccgcaa tggagcacgc ccacaagcgc gccgcggaaa tggccgaccg
     3541 ctacgcccgc agcgccgaca gcatccaggc gacgctcggc gaaagcctgc aaaaagcggc
     3601 caacgccgcc ggcgtggtgg agcagcagat taccaccatc atggatttgg gcgccaagat
     3661 tcaggaactg ctcaaggtgg aacaggcgct cgagcgcgcc atggcgggca ttgccggttc
     3721 cgaagagttc cggttggcct tcagccaatt gcgcgaacat ctggccacca ccgacgaatt
     3781 ctgtcgccgc ttgagcaagc cgcgcgtcat taccctgcac gaagaagtga tctcctgagc
     3841 ggggagggcc gaagggtgct cggcatgcgc aagcaactct caacccggtg gctcgccata
     3901 ggctttcttg cgtggggcgg gtgggcggga atggcctctg gccagaccct taccggccgg
     3961 gtgctggcga gtggcttgca agagccgacc ggcacggcgg tgcatcccat ctcgggcgat
     4021 gtgtatgtat ccgagcaggc caccgggcga atcctcgtct tgaaaaacgg caaggctgaa
     4081 tccgcgatgg cggccgactg gggagtctcc aacgtcctgc cgcgctgggc catttccgcc
     4141 gaaatgccga ccgaaaaatg gatggaagcc aaactcaaca agccgggtgc cattgcgttt
     4201 accaccaatg gcactctgtt tgtgacggaa cagattccca atgggcgcat tctggagcta
     4261 taccccgacg aacacggatt gttcaccact gctgtcggcg tgccggtgcc gtggttggat
     4321 caggagttcc aatggcgcaa tatttttgtg gatcccatcg gtcgcgtgtt cattgcaggg
     4381 gcggacgaga tcggctccga tttcatgaag ttcggttccg tgctcatgcg cgaagccaac
     4441 ggcaactggt gggtgattga cttcggtccc ttcgctaatt tcaccgccct cgcgctgtct
     4501 gagcggctgg atgtgctgct ggtgggcgac cgcaccaagg gcaacctgtc gtggtgggaa
     4561 gtggatcggc acatgatgct gggcggcgcg ccggatgtgc tcggtcgcgc ggacatggcg
     4621 gccttggcgg tgtatcccga cggcacattc atcattgccg cgcaggaggg gacggggaag
     4681 gccacgctgc ggcgttttga accctttacc ggtcagcagc agacgctggc cagcgacttg
     4741 acctcggtcg gcgccattgc catggaccgg ccccgcgcgc gctatatcgt cacggacccc
     4801 gccgcgggca aggttgtgga atatacgccg ccggccggcc tcaagttcaa tgaatccgtc
     4861 atgcggcaga ttgttcgttc cgtggagggc atggtcggca tgccgtcgga ggcaccggca
     4921 ttcctgaata acttttttga acggttgcag gatgcggcca aggagatttt gccggacgat
     4981 tccacccacg cggtggactt caacttgagc gacattgccg gtaaaatgcc ggtggtggcc
     5041 gggcgagtgc gggccaccgt ggaagtcgag ggtgcagaag acgaccctct ccaaagtgtg
     5101 gagtttttcc tgctctttcc gagcaaagtg gtgctgactg aatccgccgt cagtcccagc
     5161 cttagtttct tctccgcccg ccgcaagagc ggtaaactgg agcaaacccg cccggttttc
     5221 caggggaatg tgggcgtgta ccggatgtcc gggaccaact attcccaggt tgcctctgcg
     5281 caaggcggcc tccacattcc catcgtggtg tgcggcatgg atcaggccga tggcggggtt
     5341 tatgtgaacc tgtcgttcct cggcgcgggg atttacggtg attattatct gacgctcttc
     5401 caagggccgc gcgaacaaaa ggccaaattg gtcgtcaaat ccgtggaggc ggccagcggc
     5461 aacgtgacct atgaagcgtc attcatggaa gatgccgtca ttgaagggat gcaaggcagc
     5521 accattagca aggagcaata cagcaacctg ctcatttccg gttttggtgg cggcgcgggg
     5581 gccaaccgct ccgtgggctg gctcaagttg ggacagttcc ccgcccaaat gacgattgcc
     5641 ttcggcgata gcgccatgga tactgaggtg accagcgcgc aaagcggcct gaaagaaatc
     5701 gtggaaaaga agcgtgttga gttgagtttg gaaaccaccg aggatattgg cacggctgag
     5761 gaacaacacc ccgccgcgcc ggacaataag gagtccgcgc catggccatg aagatggccg
     5821 agtttgatca gtccggcttc caaaacatcg ttttgatgtt gatcggcctg atgacgctgg
     5881 tgatggtgag caacgtgctg accatcatca gcaatccgga taacatcaag atcggggcgg
     5941 tggtcaccgg gtctgtctat ggtgaggaag aagatgccga aaccttcatc ccgcccaaat
     6001 tccagaacat gaagcaggat ccgatttatc tggatgtgga gccgacccgc ctgacaattt
     6061 accccgaact caaggtgatc gaggaacgcg atctggtgtt tgaaggcaac gactttgaac
     6121 agtttttgga cgatgtggaa aaagtgaggt cgcgtcgcta catcgtgctg ctgctgcgtc
     6181 cgggcagcgc ccaattccag cgcaaactgc gcaaagtgat tcgggaccgc ggtattgatg
     6241 tgggttttga gccgtgggaa gccgggcgcg aaatccaagt ggttcgcgct tgggaagaat
     6301 tggtgccgga aggcgagggc ggcgagggcg aagctgtcga aggaaccgaa gccggagcga
     6361 ccgaaaccac tcctgccggg gagcctgctg caggcgagga gtccatgact gaagcgccgg
     6421 ccgccaccga aacgatgcct gaaccaaccg gggaaggaat gtaagccatg tccgaaggcc
     6481 ccagcattga cctgagttcc ctgaccgaca tcatgacgtg cattcttggc tgcttggtgt
     6541 tcattatcat gctcactggt attgacgcct cgcagatcac cgtgctcgtc gccacgccga
     6601 aggaaatgac ggccgacgat aaaagcccgg ttttctttga atgccgcaaa gaccagttgt
     6661 ttgaaatctc gctggatcag ttaaagaaag cctgcgacga aaaaaccgaa gaaatccgcg
     6721 actgggtcaa gggcaacgag caggaattcc tcaagcaagc ggcgcaaacc atgctggaag
     6781 tgaacggtca gcgcttggac tacacctatg ccctgatggg caaatacgtg ctgatgccca
     6841 tcatggatgc caccggctac gcactcggca ccgctacgga ggaaactccg gataaatggt
     6901 atgcctcgcg tttggcgcag attgatccgg agacccaatt catttgtttc cttgtgcggc
     6961 cggacagcta ccgggtgttc cagcgcgcgc gcaacctggc gtggatcaac aacatcaacg
     7021 tggcctgcga attgcaggac gaaaagaatc cgatcagcat cggccccggc ggcgaacgca
     7081 tgtacatgca atagccgcgg cgccggtttt cactcgtgcc tgaatctttt ttccagtgcc
     7141 agcattgcgg cgcctgttgc cgctggcccg gctccgtcct gttgaccgcc gcggatgtgg
     7201 ctaaactcgc gcggcacttg aacatgaccg agggtgaatt catccaacag cacaccacct
     7261 tggcccgcaa ccgcgcgcaa ttgacgctga aagagcaggc tgacggcgcc tgtgcattcc
     7321 tgaccgccga caaccgttgc gctgtttatc cggcccggcc caagcaatgc cgcgacttcc
     7381 cgcatggctg gacggtggcg ggttgccccg cagcgcagga accggcggtc acaaaaccgg
     7441 cggcaacagc gcctggttga gcgtcaaaaa ttgtttgcgc cattggtagt gccgcaaaag
     7501 ggaatcatcc cgccgcccca atgtgcagag tgcggcaaac agcgcctcca ccgtggcaag
     7561 gcccgcggga gggtcagagt gagcgcgaga ggtgcgggga taggccgttt gccagcccgg
     7621 cggaattgaa cgggccgcga tgggttcaac ggcacgccgc atggcggcag catggtgcca
     7681 actggcatcc agcagaagca ggggcgagcc ggcatcagcc ggggtgagcg gcggggcatc
     7741 cggcgccagc aacaagtagc ccgcaagatt ggggcgctgg cgcaacggat agctcataaa
     7801 aatcaacccc ggcgttcccc gcagggggcg caccgtacat ttgcgtggac tttcgccgcg
     7861 ccggaccaca atcatggcca gtgtttccat gcctgcgact atcgcgtatc cggccgctga
     7921 ttccaacgtc caagtgattg ctgaaccgcc gttggtgtgc ttgaatggcc gcatgacaaa
     7981 ggaaatcggc atgaaagtgg ccttgctcca aatgaatccc accgtcgggg ccttgcgcga
     8041 aaacgcagcg cgaatcctaa aactggccca cgcggcggcg gaagccgggg cggagatcgc
     8101 ggtctttccg gaaatggcct tgtgcggata ttcgcccggc gatctggtgc agcgcgcccg
     8161 ttttttggac gacgtcgcgg ccaccatggc gcaactgggg gcggagttgc ctgcgggcct
     8221 gctcgctata attggcgcgc cttggcgaaa ggacggccaa gtgatcaatg cggccctgct
     8281 gtggcgcgac ggccagttgc tagctgtggc cggaaaaact gtactggacg acagcgtcga
     8341 gtttgccgaa agccaagtgt tcgtgcctgc gtcggcaacg ctggtgcatg aggtgaaagg
     8401 cgtccgggtg ggggtgggga ttggccgttc cacgccgggg tcgggcgagg tggtaatcca
     8461 attggcggcg cagccttatc gtcatgaacg ggtcgcagcg ctgaccgaac gcgccagcca
     8521 acttgcgcag gccaccggcg tgccggtctt ggtggccaat ctgacgggca gccatgaagg
     8581 gctggttttt gccggcggca acgtggccgt ggcggcggat ggcacagttg ccacgcgcgc
     8641 gaccttgctc cgcgaggaaa tgttgctggt gaatctggtc cgaaaaaatg gcggctggca
     8701 gccggtggcc ggtgcagtgg ttcctttgcc atccgccgtg gccggcactt acgcggcctt
     8761 gcagacgggc ttgcgcgatt atgtggagaa aaacaagttt ccgggcgcaa tggtggcgct
     8821 gtccgggggc atggactccg cgctcgtggc cgccattgcg gtggatgcct tggggccaaa
     8881 ccgggtattc ggagtgacga tgccctcccc ggtgaccgcc aacgaaacat tggccgatgc
     8941 gctgggacta gcccagcgat tgaatattcc ttgcctgaaa atcccgctgg ctcccgccat
     9001 gggcacgctg caagacttgc tggaccgtgc cgatttgcag acctattggg ctgcgccctt
     9061 gcccggctcc accgcggcgg aaaatgtgca ggcgcgcctg cgcggtttgc tggccatggc
     9121 gttgtccaac cgctacggcc atttgctggt ctgcgccagc aacaaaagcg aaatggcggt
     9181 cggttatgcc acgctgtatg gcgacatggc tggcggcttg gcgctgctca aggatgtacc
     9241 caaaactttg gtgtatgaac tcgcgcattg gcgcaaccgg cagggcgaag taattccgcc
     9301 cggcactatt gcgcgaccgc cttcggcgga gctgaaggct aatcaacgcg acaccgattc
     9361 gttgccggcc tatgacgaat tggaccccat tctcgagcgg ctcatggacc gccaacagga
     9421 cgtggctgaa atcgtgaggg ctggctttgc cgaagccacc gtgcgccggg tggcgcgaat
     9481 ggtggcgcgt gcggagttca agcggcggca aagtgcgccg ggcatttggg tgacctcgcc
     9541 tcaacgccgg tggtcggcat cacgcccgtt gaccaatggc tatgcggaat agtcggacgc
     9601 ggctcggccg gaaggcaatt ccaagatgaa gttgcagccg gcgccgggcg cggtttccac
     9661 tcgcgcggtg ccgtggtggg ccagcaggat ttgctgcacg attggcaggc ccaggccact
     9721 gccgccggcg gcgcgcgagc gccccttgtc tgtgcgataa aaccggtcaa aaatgtgctg
     9781 tgccgcttcc ggtttcaggc cggggccgcg atccttgacg gataattcca gattgccgct
     9841 gggcagcacc tgcgcgcgga tttccaccgg ccccacaagc gcgtagagca cggcattctt
     9901 gatcaggttg gcgagggcca cagtcatcag attggcgtgc aggccccaga cgagccccac
     9961 ggggatatcc gtttggatgg caatatcggc ctgttgtgcc cattcggcac aaaccgacac
    10021 ggcctccgcc acgacatcag ccacaatggt cggcgtgatg ggcacaccac cgatggcgtc
    10081 gcgctcgctc tgcagccagg ccaagcgcaa gagatcgtcc acaatccgca ggatgcggcg
    10141 ggagtggcgt tcgatggtac gcaaggattg ggtgcgaaac tctggggtga cgttgggatc
    10201 ggccagcaat gtttcggtat agcctagaat cagcgtcagc ggagttttaa gttcatgaga
    10261 cacattgacg gcgtactctt cgccggcttg ttcgagctgc gtgatccgtg aaatatcgcg
    10321 cagcaccagc agcacctgtc ccggtttgcc ggcatgttcg tgaagcaggg gcaccccggc
    10381 cacgttgcac agcgaattgg atgcggtgag gcgcaggtcc accgcctgaa aagccgccga
    10441 tgtccggatc gcggtcagca gcgccaacaa atgcgcgcca tcggcgaggt ctgctagatg
    10501 ccggcccgcc aagcccggcc cggtcggtgc gagcagtttt tcggcggcag tgttggcccg
    10561 caaaatatga tcattttctt ctacggtcag cgtgccgtct tgaaacagat ccagcagggc
    10621 gtcgaagtct tcgcggccgg tggcgtgcag gatgggattc gcgcgttggg gcggcaaggg
    10681 gggcaaggcg ggggtgcgcc atgcccggac caaactggcc acaagcaaac cggccagaac
    10741 agcggccagc ccaacaaacg ccccgaagtg cgcagccaaa gtcgccaccg gcagcaacca
    10801 ccaacagatc aagccaaacg ccaacaacac ggcgagcagc acgctgctca tgatgaccgg
    10861 cagcgagaga ttggtcgtgc gcggcacgga cctagacttc cttgccgcga taccccacgc
    10921 cccgcacggt ttcgatcaaa tcgtcgcgca ggttcagttt tttgcgcagg ctgaccattt
    10981 ggacatccac gatgcgctct gtgaccgttt gcgcttcgcc gcgcacatac tccacaattt
    11041 ggctgcgact gaacaccttg cccgggtttt gcgcgagcag ttgcagaatc cggaattcgg
    11101 tataggtcag atgtacttcg cggcccttca cagtgacttg atgtcgtcca gggtcaatga
    11161 ccaacggccc gtatttgatt ggcggcgttt catcggcatc cggcttggcg atgcccgggc
    11221 gccgcaggat ggcctgcacg cgggccatga gcacagacgg attgaacggt ttggtcacgt
    11281 agtcatcggc gccgatttca aggccggtaa taatgtcttt ctcgctattt ttggcggtga
    11341 gcatgatgat ggggatggcg cgcagcttgc ggtgctgctt catcttgctg cacacggcca
    11401 agccgtccat gcccggcaac atgagatcaa gcaaaaccag cacgatggag cggcttttca
    11461 ggatagccag cgcctcttcg cccgtgccgg cgaccaccgg cgcatactcg tgcttttcga
    11521 ggtggaactt caacagttcc tgaatgtccg gctcatcctc aacgatcaat atattttcag
    11581 tagtgtccca acgtgtaagc aaaccatctt cgcaacatgt tatttatgag ca
//
LOCUS       DAOPNP010000080        11347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6574_length_11347_cov_9.084662, whole genome shotgun sequence.
ACCESSION   DAOPNP010000080 DAOPNP010000000
VERSION     DAOPNP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6574_length_11347_cov_9.084662"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..858)
                     /locus_tag="PKI64_07880"
     CDS             complement(223..858)
                     /locus_tag="PKI64_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOE00965.1"
                     /translation="MPKPKQRPARKLSPVLHILCEGTKTEPYYLGQYIHEHFPGTKLI
                     RIEKTPKRTPKELVAAAREILRCAPEGDIAWVVYDRESPTKYSDSLHAAARQKAGGKV
                     QIALSNVCFEVWLLLHFQGTCKAHDSCDDLLQRSRLKELIPDYDKANRRRYSEDEINF
                     ARQNAKQMNESTKRGANADWTQPHQWNPYTDVYKLLDAIDQFGGTYCTRPP"
     gene            complement(851..2140)
                     /locus_tag="PKI64_07885"
     CDS             complement(851..2140)
                     /locus_tag="PKI64_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE00966.1"
                     /translation="MIIEFTVSNFRSIKDDQSFSLYAAKPSGHLALNIAPTPAPGGIY
                     PLKSVGIYGANASGKTNLLQAFRALQYLVARSGSLKDGEHIDCYEPHLLAKNTKNAPV
                     RLALEFQIPNGFRYLYAIAFVQNRIVEERLDLYRTARVANLFDRGENDTWESIKFGPF
                     LKGGTRKFPFFANNAYLSKAGNSADAPEVIRDAYNYLLKDMVHLGISDEVSSEELLEN
                     EDLLQTVSQFLSLVDTGIARVGLYQDVDRAKNIIFPPDMPEDLMRRIRKEASLRFLFF
                     HQTDTAEVTTFPINAESAGTRKLFKLAPLIVNTLKDGGVLIIDELDRSMHPHMAELII
                     QLFHDPEVNPGGAQLIFSTHNANLMSRELLRRDQIWFAEKSNGASRFFSLDDFDKKKV
                     TPASPFARWYAEGRFGAIPSIDYSGVVKLLRKRRPDA"
     gene            complement(2619..5516)
                     /locus_tag="PKI64_07890"
     CDS             complement(2619..5516)
                     /locus_tag="PKI64_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE00967.1"
                     /translation="MKQKKKKNSDIEIVRGLALSYGPGMMLMETLVQAVLTYLPARRC
                     FMKLETSIHPDDLAGPSPFPDTLCDPKPSTPWPGPIAEYALELKSDGKPVTKKARSDA
                     LFWQPDSLKQLVHAIWTKSDPTMWTKVSLNTTLHEAERAVRRAVAAAQAAVPAQAEAI
                     ARMGQIMLEQACSRPFPHQESRRAPIFVFSHPGHGVTYILRAMANALQGGFFAVSQEQ
                     TSATGLIRWALGTDPAIIQIHGFATREHIAGSAVHAILRYYQYGHYVVDHVSGIVTDD
                     DEDSSEGPPQGYLLEAVLERSFVFIRVTPPIPPADAADYTPEEMVAMLREAGKQAYRS
                     GLSPAQVEDGIWDMVRNWNSVVFRRTPVRDIVEQVMREFAHARDNMPELVLAHLSRAD
                     HLRLAALFILERGFPSPKEIRGAAQRFLHSLPPAAREAKQLKFDVAEPEWEALFIADT
                     PGSEAWFARLEGLAKQFNYRGNRLTAQAVYDDTGKVLTMTRFEQRAPRAISGNAYLQA
                     RPKLRFRDVAGHEEAKQRFRQILAYFKDPEPYRRLNVIPPLRILLHGPPGTGKTLLAQ
                     ALAGETGLPFFAIAASEFMTREYAGQGATLMREFFQTAESCRPCIIFMDELDAFSARD
                     QMSDGSAGIDFRGTLNTLLTLLDGVQSAPDILVIGATNRPEDLDPALLRPGRFGTRIQ
                     IQHLSARDRDRLIRQYLTPEQCAGDYEDIVAALCRRTTGGLSPVEIVQIIHEAKLEVI
                     QQGRPHVTREDVMTQADRILLGKKFQPLQNGLRRATAIHEAGHAFLLRTLLPEQKLDR
                     LSIGHREETVGMVRILDEEDPSRFLDRDDTLCQLLCRLGGMAAEETFVGHWDLGASDD
                     FDVAVVLARYILTNIAMGDLGLHGIVRSRMREESEPDTALEAMVAQLLERCRRLAGDI
                     FKTHEPVLLKLARELELREDLEQDDVDRILAGVVIDRKAIVQQMLAPPAGS"
     gene            6085..7905
                     /locus_tag="PKI64_07895"
     CDS             6085..7905
                     /locus_tag="PKI64_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE00968.1"
                     /translation="MRIKPRYRLLALAFWCGATTGFPQTAPPLGCFPHTNGTTFRVWA
                     PNATNVALGGDFNNWQAAAMRPETDGLWSLTIPAAQPGQHYKYLINNTLWKRDPRARQ
                     VNHSNGDSIVYDPAAFDWGRDRPPRSARNDWVIYQLHIGTFAGRPLPATFDDAIPYLD
                     HIRALGCTAVAPLPVNEFPGAFSWGYNPSDPYAIETDYGGPAAFKRFVHAAHARGLAV
                     IADVIHNHYGPTDLDLWRFDGAFWNGEYGGIYFYNDSRAETPWGHTRPDFGRPEVRAY
                     IRDQIFMYADEYHVDGFRWDSIYHIINSGWGPNPEAIQMLHDINRELAHSRPDILRIA
                     EDHGFDHDMGFDAQWDVGHRWALFNQLVTAHDPERDMHTVAGTLTDWPGFQRIIFTEA
                     HDYVARMNENRSRVPTMIHPEDPTSIWARKRALLGAAMIFTTPGIPMIFQGQEMLETR
                     AFHDDVPMNWSLTNTYAGNVQAYADLIRLRRNLNGATPGLQGSGVYVQHLDDEQKVIA
                     FTRWNQGGATDDVVVIANFSSHDFTANDYSIRFPTAGTWHRQFNSDSTCYFADFGDVG
                     ADQVEATGNPPAAPVNMGRYSLQIFSKNAPDSPPPATISD"
     gene            7969..10344
                     /locus_tag="PKI64_07900"
     CDS             7969..10344
                     /locus_tag="PKI64_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00969.1"
                     /translation="MKRFLMVGLFLFTTAVMARAFQFENLHHFETPPYQPEAALTQGP
                     DRRLWGTTCYGGTHDCGAIFKSSTNGGYATVWNFTGGPDGSFPQAALLLASDTNLYGT
                     TSAGGTGGAGVIFRITSAGVLTVLHSFSGGTGGSSPCGGLIQGADKALYGITTYGGNN
                     SCGTVFKITTNGTFTQLWSFTGGDDGATPYGKLVQGPDFALYGTCSRGGSNDWGTVFR
                     ITTSGAMKPLWSFTKGADGAQPFGGLAAGTNGVFVGQTYSGGNNGYGTIFNITTNGTL
                     TSLVSFNQSNGANPRSGLIRATNGFFYGTTQFGGHSGKGIIYKIGQHGGMTTLWSLDP
                     QTEGSEPNSALFQMPSGAFYGVSPRGGAYDTGTMFEYLESAEDVKCLRSFPPAPMGRE
                     PSAALVQGSDGAFYGTALWGGTNNFGTIFKLDTNGVVSCLWPFTGGDDGANPGAELLP
                     LPDDGGLALYGTTQRGGISNCGTAFVLYSDGTGNAYSFTNSPSDGNNPRGGLVRGPDQ
                     AFYGSTYLGGISNKGTIYRIATNGEESLLYSFRGTDGANPRGTLAVASNGVFYGVTER
                     GGANDMGTLFRITTNGVFTTLWNFSGDSDGGAPRAGVILARDGHLYGTTVRGGIEDAG
                     TIYRITTNGVITILHSGSWGPNGGRIQAPLVQGRDGNLYGTTLFGGDNDCGVIFRITT
                     DGVFTRLWSFAGGIEGGLPQAGLIEAGNWFYGTTPMNNGTIFRFSMGPALTTAGPSGL
                     SARVLLWDQPGFQLQSTTNALGPYTTLLPVATSPYTNKFNDRLRFFRLRQE"
     gene            complement(10449..11270)
                     /locus_tag="PKI64_07905"
     CDS             complement(10449..11270)
                     /locus_tag="PKI64_07905"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE00970.1"
                     /translation="MIVVSCRQGFGSDCKLAGRLLVREYPDPARLDELRDVGMSELRD
                     RAAGQTVGVLVHGYNTKLSEVLTAYGEMQERMTRAGLLGRQGCYGLLVGFAWPGWTRV
                     AFLLARRAANQAGLFLRELMNELRGTAQAVDVQTHSLGARVALSALRKKDGTAARHVL
                     LSAAAVDYTILEPGRFFQPSLRACARCVVYHSRRDKVLRQAFPVGDSPDGIQPALGLN
                     GPRSRAITLRACPNVHVVDCAAVVGEHSGYRKADAYYQHWYRVASGAALERYEKL"
BASE COUNT         2352 a         3592 c         3041 g         2362 t
ORIGIN      
        1 aaaatacgag ttaaaactaa accgttacaa gtctgagggc taccactccc gaaaatgcaa
       61 aataccgtac gttaactgcg gcagtcgaac ccatgtgtcg ggccatgatt caactgcacc
      121 ccctaatatc acaggacaca atgggctggg ttccacatcg aatgtgctat cttatcgcct
      181 accaagcgga atcaacagtc tgggttcagg attcgggcat gctcaagggg ggcgagtaca
      241 gtacgttcca ccgaactgat caatggcatc gagcaatttg tacacgtcgg tgtagggatt
      301 ccattggtga ggctgggtcc agtcggcatt tgctccacgt ttggtagatt cgttcatctg
      361 tttcgcattc tgccgagcaa agttgatttc atcttcagaa tacctacggc gattcgcctt
      421 gtcatagtcc ggaatgagtt ccttcagccg actgcgttgc aacagatcat cacaagaatc
      481 gtgtgctttg caggttcctt ggaaatggag caacagccag acttcaaagc agacattgga
      541 cagtgcgatc tgtacttttc cacctgcttt ttggcgagct gccgcatgca gagaatcgga
      601 gtattttgtc ggcgactccc ggtcgtatac aacccaagcg atatcgcctt ccggcgcaca
      661 gcgaagtatt tcacgcgccg ctgcgacgag ttccttgggt gtccttttgg gtgtcttttc
      721 gattcgaatc agcttcgtgc ccggaaaatg ctcgtggata tattgcccca gataatacgg
      781 ttccgtcttg gtcccctcgc acaagatatg caagacgggg ctgagtttcc gtgcggggcg
      841 ctgtttgggt ttaggcatcg ggccgccgct tccgtaacaa tttgaccacc ccgctgtaat
      901 caatcgaggg aatggcccca aaacgaccct ccgcgtacca ccgggcaaac gggctggcgg
      961 gcgtgacctt tttcttgtcg aagtcgtcca aggagaaaaa gcgtgaggct ccattcgatt
     1021 tctccgcgaa ccagatttga tcccggcgga ggagctcccg tgacattaga ttggcattgt
     1081 gtgtggaaaa aatgagctgt gcacccccag ggttcacctc gggatcatgg aacaactgga
     1141 tgatcagctc cgccatgtgc gggtgcatgc ttcggtccag ctcgtcaatg atcagtaccc
     1201 cgccatcttt aagggtgttg acgatcaacg gcgccaattt aaataatttt cgagttcccg
     1261 cagactcagc gtttatgggg aaagtggtga cctctgccgt gtccgtttga tggaagaaca
     1321 gaaatctcaa agatgcttcc ttgcggatac gtctcatcaa atcctctggc atatcaggcg
     1381 ggaaaatgat gtttttggct ctgtccacat cctggtacaa gcccaccctg gcaatgcctg
     1441 tgtccaccag tgaaagaaac tgtgaaacgg tttgcaatag gtcctcattc tccagcagct
     1501 cctcggatga cacctcatcg gatattccga gatgcaccat atcctttaac agataattgt
     1561 aagcatcccg aataacctca ggggcgtccg cgctgttgcc agccttggac aaataggcat
     1621 tattggcgaa gaatggaaat ttccgggtgc ctcctttgag aaacggcccg aatttaatgc
     1681 tctcccacgt gtcgttttct ccgcgatcaa acagattggc aactctcgcc gtccggtaca
     1741 aatccaggcg ctcctccaca atccggttct gcacaaaggc aatggcgtac aaataacgga
     1801 atccattggg aatctggaac tccagcgcca atcgcacggg cgcatttttc gtatttttgg
     1861 ccaacagatg gggctcgtaa caatcaatgt gctcgccatc tttcaggctg cccgaacgag
     1921 ccaccaggta ctggagcgca cggaaagcct gcaacagatt cgtcttgcca gaggcattcg
     1981 cgccatagat acccacgctt ttcagcgggt agatgcctcc cggagcgggg gtgggggcaa
     2041 tattcagagc cagatggccg ctcggtttcg ccgcatacag agaaaacgac tgatcgtcct
     2101 tgatggatcg aaagttggat actgtaaact caataatcat attaaattgt tatctatttc
     2161 cacagatgtt tgtccgtatt tatacagaca aatatgcact atatgagata gcaatcaaga
     2221 aaaatattgc tttttgaatc atttttttgc atccatttgc gagagtaaga gatagggtag
     2281 cgttgatccg ttcttccctc ggtgctggat caatcggccc tggctttggc ttcggttgcg
     2341 cgcatcggcg attttcttgg gctggctgct ctcgccaaaa ggataacccg aatagcccca
     2401 acacccggtc cctcagctcg gactacgccg ctccgctgag ctcctcctgc gtcccgatgg
     2461 ctccgtcatc caaatccgcc gcgctgaaac cctcgccgac ctcttcgcca ccctcgacct
     2521 ccccgccgcc gccgccttcc attgacccta cggtcagatt gctaggctgg catggtcaca
     2581 gaccttccag ccgtttctgc ccaccaaaac aacagctttc acgatccggc gggtggcgcg
     2641 agcatttgct ggactatggc cttgcggtct atgacaacgc cggcgaggat gcgatccacg
     2701 tcgtcttgct ccaaatcttc gcgcagctcc agctcccggg ccagtttcaa caagacgggc
     2761 tcgtgggtct tgaagatgtc gccggccaaa cggcggcagc gttccaagag ctgggccacc
     2821 atggcctcca gcgccgtgtc gggctcggat tcctccctca ttcgcgagcg tacaatgcca
     2881 tgcagaccga gatcccccat ggcgatattg gtcaatatat agcgggccag gacgaccgcc
     2941 acgtcgaagt cgtcgctggc gcccaagtcc cagtggccga caaaagtttc ttccgccgcc
     3001 atgccgccca aacggcagag taattgacac agggtgtcat cccgatcgag gaagcggctc
     3061 ggatcctctt cgtccaaaat gcgaaccatg ccaacggtct cttcgcgatg accaatggac
     3121 aggcggtcga gtttttgttc gggcagcagc gtgcgtagca ggaaagcatg gccggcttcg
     3181 tggatggcgg tggcccggcg cagcccattc tgcaaaggct ggaatttctt gcccagcaaa
     3241 atacggtcgg cctgcgtcat cacgtcctcg cgggttacgt ggggccggcc ctgctggatg
     3301 acctcaagct tggcttcatg gatgatctgt acaatctcga ctgggctgag gccgccagtc
     3361 gtgcggcggc acagggcggc cacgatgtct tcgtagtctc cggcgcattg ttcgggggtg
     3421 agatactggc ggatcaaccg atcgcggtcg cgcgccgaca agtgctggat ttgaatacgg
     3481 gttccaaaac ggcccgggcg caacagggcc gggtccaagt cctcgggacg attcgtcgcg
     3541 ccaatgacga ggatatccgg cgcggactgt accccgtcaa gaagcgtcaa cagcgtattc
     3601 aacgtgccgc gaaaatctat tcccgccgag ccatcggaca tttggtcacg ggcgctgaag
     3661 gcgtcgagtt catccatgaa aatgatgcag gggcgacagc tttctgccgt ctgaaaaaac
     3721 tcccgcatca gggtcgcgcc ctggccggcg tattcgcgag tcatgaactc gctggcggca
     3781 atcgcaaaaa aaggcagccc ggtttcgccg gccagcgcct gggccagcag ggtcttgcct
     3841 gtacccggcg gtccatggag caggatgcgc agaggtggaa tcacgttcaa gcggcgatag
     3901 ggctccgggt ccttgaaata ggccaggatt tggcggaacc gctgcttggc ttcttcatga
     3961 ccggccacat cccggaagcg cagtttcgga cgcgcctgca gataggcatt tccggaaatg
     4021 gcccgcggcg cacgttgctc gaatcgggtc atggtcagga ctttgccggt atcatcataa
     4081 acagcctgcg cagtcagccg gttgccacga taattgaatt gcttggccaa tccttccaga
     4141 cgcgcgaacc atgcctcgga gccgggagta tccgcgatga ataaagcctc ccactccggc
     4201 tccgccacgt cgaatttcaa ctgcttcgcc tcgcgcgcgg ccggtggcag ggaatgcaaa
     4261 aaccgctggg cggcgccgcg aatctctttg ggcgacggaa atccgcgctc caggatgaag
     4321 agcgccgcca aacgcaaatg gtcggcgcgg gacaaatggg ccaagaccag ctcgggcatg
     4381 ttgtcccgtg cgtgcgcgaa ttcccgcatg acctgctcaa cgatatccct cacgggtgtg
     4441 cgccgaaaga ctaccgaatt ccagttgcgc accatgtccc agatgccgtc ctccacctgt
     4501 gcgggtgaca gtccgctccg ataagcctgc ttcccggcct cgcgcagcat cgcaaccatt
     4561 tcctccggcg tgtagtccgc ggcatctgcc ggcggaatgg gcggagtcac ccgaatgaaa
     4621 acaaacgacc gctccagaac cgcttcgagt aggtagcctt gcggggggcc ttctgaggag
     4681 tcctcatcgt cgtcggtgac tatgcctgac acatgatcca ccacatagtg accatactgg
     4741 taataccgga ggatggcatg cacggccgag cctgcaatgt gctctcttgt ggcaaagcca
     4801 tgaatctgga tgatggcagg atcagtcccc agagcccagc ggatcaatcc ggttgcgctg
     4861 gtctgctctt ggctgaccgc gaagaagcct ccttgcaacg cgttcgccat cgcccgcaaa
     4921 atataggtca cgccatgacc cggatggctg aaaacaaaga tgggcgcacg ccgggactcc
     4981 tgatgaggga aaggtcgcga acaagcctgc tccagcatga tttgccccat ccgggcgatg
     5041 gcttccgcct gagccggcac ggcggcttgc gccgcagcca cggctcgccg gactgcgcgt
     5101 tccgcctcgt gcagcgtagt attcaaactc accttggtcc acatggtcgg atccgatttc
     5161 gtccaaatcg catggaccag ttgcttcaac gaatcgggct gccaaaagag ggcgtccgac
     5221 ctcgcctttt tggtgaccgg cttcccatca cttttcagct ccaaggcata ttccgcaatc
     5281 ggacccggcc agggtgtgga cggcttggga tcgcacagtg tgtcgggaaa ggggcttggg
     5341 ccggccagat catccggatg gatggaagtc tccagcttca taaaacagcg acgggcaggc
     5401 agatatgtca gcaccgcctg caccagagtt tccataagca tcatccccgg gccataggac
     5461 agtgccaaac ctcgcacgat ctcaatgtcc gagttctttt tcttcttttg tttcatgggc
     5521 gccctcccgt cggctggccg tatatacaga ttttacccat ccagatgcta cttctcaagt
     5581 acacatataa tggcgtaccc gccatttccc caaacccgta acctcggcgc cgctttccat
     5641 tgaccctgat acggccggat tgtcgggctg gaacggtcgc aggccttcca gccttgcccg
     5701 accaaaacac tacgctgtcc agagtttata aactctgggc atatattgta gtatttctat
     5761 ttcacttata tacaatttgt tatgtctaat tattgggcta tttttaatca gccgttttta
     5821 tggtgatgtc cacactttat aaactctgga caaatttatg gcgggtgatt tttgcgtttt
     5881 tatctgtgcg gtgccaaccc gcacggtgat tgtcgccccc cccactctcg cggaaaaatc
     5941 ggcaaagagc caagcggccg gattgtcggg ttggaatggt cgcaggcctt ccaaccgttc
     6001 ccggcggacg ctttgttgcc aacccgcgaa attgggtttg ttgtgtgccg ccgggtatgg
     6061 ttcgatctac cgcaaggtga acgcatgagg atcaagcccc ggtaccggct actggctctc
     6121 gccttctggt gcggcgccac caccggcttc ccgcaaaccg ccccgccgct gggctgtttt
     6181 ccgcacacga atggaaccac cttccgcgtt tgggcgccca acgccaccaa tgttgcgctc
     6241 gggggcgatt tcaacaactg gcaggccgcc gccatgcggc ccgaaaccga tggactctgg
     6301 agcctcacca tccccgccgc gcagcccggc cagcactaca aatatctcat caacaacacg
     6361 ctctggaagc gcgacccgcg cgcgcggcaa gtcaatcact ccaacggcga ttccattgtc
     6421 tatgatcccg ccgccttcga ttggggtcgg gaccgccctc cccgctctgc gcgcaacgac
     6481 tgggtgatct atcaactgca catcggcacg tttgccggcc gcccgctgcc ggccacattc
     6541 gatgacgcca tcccgtatct cgaccacatt cgcgcgctcg gctgcaccgc cgtcgcgccg
     6601 ctgccggtca acgaatttcc cggcgcgttc agttggggct ataacccgag cgacccctac
     6661 gccatcgaaa ccgattatgg cggccccgcc gctttcaaac gctttgtcca cgccgcccac
     6721 gcgcgtggcc ttgccgtcat tgccgatgtc atccacaacc attacggccc caccgacctc
     6781 gacctctggc gcttcgacgg cgcgttctgg aatggcgagt acggcggaat ttatttttac
     6841 aatgattccc gcgccgaaac gccgtggggc cacacccgcc ccgattttgg ccgccccgaa
     6901 gtccgcgcat atatccgcga ccagattttc atgtatgcgg acgaatatca cgtggatggc
     6961 ttccgctggg attccatcta tcacatcatc aattccggat ggggccccaa ccccgaagcc
     7021 atccagatgc tacacgatat caaccgcgaa ctcgcgcact cccggcccga tattctgcgc
     7081 attgccgaag accatggctt cgaccacgac atgggcttcg atgcccaatg ggatgtcggc
     7141 caccgctggg cgctgttcaa tcagctcgtc accgcccacg accccgaacg cgacatgcac
     7201 accgtcgccg gcaccctcac cgactggccc ggcttccagc gcatcatctt caccgaggcc
     7261 cacgactacg tggcccgcat gaacgaaaac cgcagccgcg tacccaccat gatccacccg
     7321 gaagacccca ccagcatttg ggcccgcaag cgcgccctgc tcggggcggc catgattttc
     7381 accacccccg gcattccgat gatctttcag gggcaggaaa tgctggagac ccgcgcgttt
     7441 cacgacgatg tgcccatgaa ctggagcctc accaacacct acgccggcaa cgttcaagcc
     7501 tatgccgacc tgatccgcct gcgccgcaat ttgaatggcg ccacccccgg cctgcaaggt
     7561 tctggcgtct atgtgcaaca tctcgatgac gagcaaaaag tcatcgcctt cacccgctgg
     7621 aatcagggcg gcgccacgga cgatgtcgtg gtaattgcca acttttccag ccacgacttc
     7681 accgccaatg actactccat ccgcttcccc accgccggta catggcaccg ccagttcaat
     7741 agtgattcca cctgttactt tgccgacttt ggcgatgtcg gcgcggacca agtggaagcc
     7801 accggcaacc cgcccgccgc ccccgtcaac atgggccgct acagcctcca gatattctcg
     7861 aaaaacgcgc cggattcccc gccgccggca accatttcag attgaccggt tgatgaccgc
     7921 ggggatacgc tatcggcgtt gaggttaagc atacaggcgg agggtgccat gaagcggttt
     7981 ctgatggttg ggttgttcct gttcacaacc gccgtgatgg cacgagcctt tcagttcgag
     8041 aatcttcacc atttcgaaac accgccatac caaccggaag ccgcactgac ccaaggcccg
     8101 gatcgccggc tttggggaac aacgtgctat ggcgggaccc atgactgtgg tgcaatcttc
     8161 aagagctcaa ccaacggcgg atatgctacg gtatggaact ttaccggcgg cccggacggc
     8221 tcctttcccc aggccgcctt gttgttggcc agcgatacca acctgtacgg cacaacctct
     8281 gccggtggga ccggcggtgc tggcgtcata ttccggataa cctccgcggg ggtcctaacc
     8341 gtccttcatt ccttctccgg cggtaccggc ggcagttctc cctgcggtgg attgattcaa
     8401 ggcgccgata aagcactgta tggcatcacc acctacggag gaaacaacag ctgcggcacg
     8461 gtgttcaaaa tcacgacgaa cggcaccttc acgcaactgt ggtccttcac gggtggagat
     8521 gacggcgcaa caccctatgg caagctggtg cagggtccag atttcgcgct ctacggcact
     8581 tgttcccggg gcggcagcaa cgattggggc acggtgttcc gcatcaccac cagcggcgcc
     8641 atgaaaccgc tgtggtcgtt cacaaaggga gcggatggcg cccagccctt tggcggattg
     8701 gcggccggca ccaacggggt attcgtgggt caaacctata gcggcggcaa caacggctat
     8761 ggcaccattt tcaatatcac caccaacggg accttgacgt ctcttgtgtc gttcaaccaa
     8821 agcaacggcg ccaatccccg ctccggactc attcgcgcca cgaatggatt cttctatggc
     8881 acgacacagt ttggtggcca ctccggcaaa ggtatcattt acaaaattgg gcagcatggc
     8941 ggaatgacca ccctgtggtc gctggacccc cagaccgaag gatcggaacc caattccgcg
     9001 ctgttccaga tgccttctgg cgcgttctat ggcgtgtctc cacggggggg cgcttacgac
     9061 acaggaacca tgttcgagta tctggaatcg gccgaggatg tgaaatgcct gaggtccttc
     9121 cccccggcgc ccatgggccg ggagccttcc gccgctctcg tacagggcag tgatggcgca
     9181 ttctacggca cggcgctctg gggcgggacg aacaatttcg gcaccatttt caaattggac
     9241 accaacggtg tggtgtcatg cctctggccg ttcacaggcg gagatgacgg cgcaaatccc
     9301 ggcgcggagc tgctgcctct ccccgatgac ggcggcctcg cgctctatgg cacgacccaa
     9361 cgtggcggta tttccaactg cggcacggcg tttgtgctgt attccgatgg caccggcaat
     9421 gcttattcgt tcaccaattc accttccgac ggcaacaatc cgcggggtgg attggtgcgc
     9481 ggccccgatc aggctttcta tggcagcacc tacttgggcg ggatcagcaa taagggcaca
     9541 atctatcgaa tcgcgaccaa cggggaagaa tccctgctgt acagtttccg cggcaccgat
     9601 ggcgccaatc cgcgcggcac cctcgcggtc gccagcaacg gcgtattcta cggcgtgacg
     9661 gaacgaggcg gcgccaacga catgggtacg ttgttccgca tcactaccaa cggtgtgttc
     9721 accacactat ggaatttcag cggagattcg gatggaggtg cgccccgcgc cggcgtcatc
     9781 ctcgctcgtg atggccacct ctatggcact acggtccgtg gcggcatcga agatgccggc
     9841 acaatctatc gcatcaccac caacggcgtg ataaccatcc tgcattccgg ttcttggggc
     9901 ccaaacggcg ggaggataca agcgccgctg gtgcagggcc gggacggcaa cctgtacggc
     9961 acgaccctct ttggtggcga taatgactgt ggagtgatct ttcgcatcac taccgacgga
    10021 gtattcacgc ggctgtggtc tttcgctggc ggtattgaag gcggattgcc ccaagccggc
    10081 ctcatcgagg cgggcaactg gttttatggc acaacaccga tgaacaacgg cacgatcttc
    10141 cgtttctcca tgggcccagc tctcaccacg gctgggccgt ccggtctctc ggcgcgagtg
    10201 ctgttgtggg atcaacccgg ctttcaactt caatccacca ccaacgcgct gggcccctac
    10261 accaccctcc tgcctgtcgc caccagcccc tataccaaca agttcaacga ccgcctccgt
    10321 ttcttccgcc tccgccaaga gtaattctcc ggaagttccg ccaccccgaa aatacccttg
    10381 tcatcgaatt gccgggccga aacggtcgca gaccttcccg ccacagaggt tttgcatcag
    10441 cagcagactc aaagtttttc gtagcgttcc agcgccgccc cgctcgccac gcgataccag
    10501 tgctgataat aagcatccgc cttgcgatac ccgctgtgct ccccgaccac ggcggcacaa
    10561 tccaccacat ggacgttggg gcacgctctc aacgtgattg cccggctgcg cggcccattc
    10621 aaccccaagg ccggctgaat cccgtccggg ctgtccccca ctggaaacgc ctgccgcaac
    10681 accttgtccc ggcgcgagtg atacaccacg caacgcgcgc acgcccgcaa cgatggctgg
    10741 aaaaatcgcc ccggctccaa aatcgtgtaa tccactgccg cggcggaaag cagtacatgc
    10801 cgcgcggcgg tgccatcctt cttgcgcaac gcgctcaacg ccacccgcgc accgagggaa
    10861 tgggtttgca catccaccgc ctgcgccgtg ccgcgcaact cattcatcaa ctcgcgcaga
    10921 aaaagccccg cttgattcgc cgcccgccgc gccagcagaa acgcgacgcg cgtccacccc
    10981 ggccacgcaa accccaccag cagcccgtag catccttgcc gtcccagcag cccggcccgc
    11041 gtcattcgtt cctgcatttc gccatacgcg gtcaacacct cggacaactt ggtgttgtag
    11101 ccatgcacca gcacccccac cgtttgccct gccgcccggt cccgcagctc ggacatcccc
    11161 acgtcacgga gctcatccag ccgcgccgga tcgggatact cccggaccaa caaccgcccc
    11221 gccaacttgc agtccgaccc aaacccctga cgacaactca ccacgatcat gcccccattt
    11281 tctcataatc cccgcctaac gccacctttt ccacaccgtg gaacttttca gcccgttttt
    11341 tccacag
//
LOCUS       DAOPNP010000081        11171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6692_length_11171_cov_8.147355, whole genome shotgun sequence.
ACCESSION   DAOPNP010000081 DAOPNP010000000
VERSION     DAOPNP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6692_length_11171_cov_8.147355"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKI64_07910"
     CDS             complement(<1..602)
                     /locus_tag="PKI64_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOE00971.1"
                     /translation="MTSLRTTGYALFTLLTLLQPLSAAPKLIINPETFDFGEVPSGES
                     VQATFTLRNAGDEPLHINHVRTSCGCTTSRLDKRELAPGEETPLTATLSLRNRSGPQH
                     QRVTIASNDPDAPSRQAHIQGTAVQYLTLTPSAVLLFSAPQGQSITRTLSLHSENQTP
                     FQLLSIEVTHPNLTVAVTPVSPSSYRLALTIPATWPAGRI"
     gene            complement(599..1378)
                     /locus_tag="PKI64_07915"
     CDS             complement(599..1378)
                     /locus_tag="PKI64_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOE00972.1"
                     /translation="MSLRYSLLSLLLLARATFGLAQTNPSDLILLRNGRELSGRLIQE
                     TSAGYVLIPAGAPTQIQRIPREATAYVLYADPAQAAKLLGLDSVARNLSKAPAPAEVR
                     LLAAEPFGKALLEAARSATNSIWISAYYFSDSRAAPIKDFYATLREKAAAGVEVVILV
                     ENSTASPPAMRRANANFAAQLSGDGIQTLLWSGTNKALHKKLVIIDGRILLLGSSNLS
                     LSGTYGSIEMNILTEAPHLVQPAIADFQRLCTQLKQRQAAP"
     gene            complement(1375..3291)
                     /locus_tag="PKI64_07920"
     CDS             complement(1375..3291)
                     /locus_tag="PKI64_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE00973.1"
                     /translation="MHDFGHLGISASRHLGISASRHLASRIARHVSFLITSLLLPISS
                     LAATALDVRGVSVTFSPQGDSLSEIIRTLHSAKRRIDVAPFFLSHTNLIDTLCFLSAK
                     QKLKVRCFMDPASTAPAECHVLDRLAQNGVEVYVVQVPDGKMHLKCAVVDDGVVITGA
                     ANWTVRGFENNVEDSLFLQSPALAQAYRARMDRLLPISELYHAPRLDDSERFESKPSK
                     LSQSKPPTLTYAAPRLQRFSSVRSARVFFSSDSEPDNGLLASETILSRIHAAQKRVDV
                     AMYLLVEPTLVEALAQKATEGKVPVRLLVDSGMLDSSSRTVLETLVAAGVQVNWLGNE
                     KFSMHLKTLVLDEAQVWTGSANWSQSAFAHNIEDLVLIESADLARAYTRHFDTLHAAA
                     TPFQPASLSVSAPSNAPASAEFPIGLPPTGPRTNWPSGLQDVASEDLLLSASARYLAD
                     EEYLPALLDLIRNAHQSVLASMYVMGEPQKDQPNLNQLTAALAQAVQRGVYVHLVLHM
                     PLSEAVTMNQIHLDWAENLRAKGVDVRLHIPTIPLHEKVVVVDLAKVLIGSHNWSEGA
                     LSSQKVTESGVLLVLPQQDRRLADHILNRESIRDMRTPALWQEELRTLRQLGNTSGKE
                     RDTLLDQLEPEEMP"
     gene            3494..4489
                     /locus_tag="PKI64_07925"
     CDS             3494..4489
                     /locus_tag="PKI64_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE00974.1"
                     /translation="MAERLLEVKDLRAGFATPEGFVRAVDGVSFEIRRGEVLGLVGES
                     GCGKTVTALTLLRLLPRPPAVIEGGQALFHGRDLLTMPTEELRTIRGGKIGMIFQEPM
                     TALSPLHRIGDQLAETARLHRRLSAAEAWALGLEWLKRVGLPDAAQRMKDYPHQLSGG
                     MRQRVMLAMTMLPEPELIIADEPTTALDVTIQDQIFDLLLKLRGGETAVLLITHDMGV
                     VWEVCDRVHVMYAARLAEKGTREQLFSTSAHPYTRGLLAAMPRLHGRAERLPDIPGQL
                     PSLRNPPPGCRFAPRCPLARAVCRQHAPALRELGGGHEVACWDGIWDQAPGAEHG"
     gene            4482..5852
                     /gene="rimO"
                     /locus_tag="PKI64_07930"
     CDS             4482..5852
                     /gene="rimO"
                     /locus_tag="PKI64_07930"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOE00975.1"
                     /translation="MASRAPRFSLVNLGCPKNTVDSEGILGSMVLAGFQLVDDPATAD
                     ICLVNTCGFLAASRQEAAGVLAELAAQRGRRRRPLLVALGCMVERAGGAPTLADGLAA
                     ADARVGFADYVKLPEICRNLLAGKRAAARARGYTGRTLPKTYLAWLDRPRLRIGSVAA
                     AYLKVGEGCSNCCAYCSIPLIRGRRASRPLAAVVKEARELVASGAKELNLIAQDTTAY
                     GVDWAGRPQLARLLRELLAMPDDVWFRILYAHPKHLSAEVLAVMAGDARVCPYLDLPL
                     QHVHDRMLRRMARGYDRGRVDDVLRMIGHALPNAALRTTFIVGHPGEGDAEFCELLEF
                     VEQGHFDHVGVFCWSPEPGTASTAMRERVPPEVAEERRAALLTAQAAVSARRLRARLG
                     RETRLLLEEKIKRGWRGRTPWQAPEVDGHTTLVGSRRGLQAGDFVTAQIVHTDVYDCR
                     ARQVMP"
     gene            5888..6952
                     /locus_tag="PKI64_07935"
     CDS             5888..6952
                     /locus_tag="PKI64_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00976.1"
                     /translation="MGLLCLLAVAARAEEIADTTPPAIAKPERSRILRLNLEEIYYLQ
                     GLELEEQSSHEHAVITRENNVTSWLDRQHGRLFRWLDNRVRWVDMAWLGEKRAYDPEL
                     STFRLSTMTRVGGRSSEKEFDIKVRFRGDFALPALSHRLHVFLDNVGRESLPGKDPMQ
                     QDSNTRLGIRAMLKTLKNSELSANGGLRWRRSHPVAYAGVDWRYARPVGDAVYAFTPR
                     VVYYTDDGFAQQAIMTWTRQVGERKIFQVRLGERSAENMDGVEFEQSLRFAWLRSGRG
                     RGWVAQASVFPHLESSDWYWDDSLINVAWRDALYRKWIYYTIIPQLQFPKEDQYEPRP
                     SLRIGLDILFGGRIGELLSH"
     gene            7081..9225
                     /locus_tag="PKI64_07940"
     CDS             7081..9225
                     /locus_tag="PKI64_07940"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOE00977.1"
                     /translation="MNESTLLFAALGSAVLALAFAWWRTQWINKQDPGTARMQEIGAA
                     VREGAMAFLRREYKVLAIFVIVVAGLLAYLNVKVTGQKNLSLVALSFVVGALCSVLSG
                     YFGMRVATAANMRTTNAARKGLPEALAVAFAGGSVMGMCVVGLGLLGLTGLTLVYFKM
                     FGTENEMLNGTVLPILTGFSMGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPED
                     DPRNPAVIADNVGDNVGDVAGMGADLFESYVGAIVAAMVLAAVSSIPAGAKGSEMNLV
                     MLPLLLAGVGILFSILGTFMVRTREGGNPQNALNAGSLSAAVLTAAASYVVIAKFAPS
                     AFSVAALPGMAAKVYNANGIFWATLAGLASGVAIGFLSEYYTAEGKGPSNRIADASST
                     GAATNIIAGLGNGMMSTALPVIVLAAATMISYMTAGLYGIAIAGLGMLATTGIQLAVD
                     AYGPIADNAGGLAEMAHLEKEVRQRTDKLDACGNTTAAIGKGFAIGSAALTALALFAA
                     FGQTVKLDKIDVMDPNVTAGLLIGAMMPFLFSAFAMQAVGQAAFAMVEEVRRQFKEIP
                     GLLEGKEGARADYARCVDISTAAAIKKMTVPALLGVLTPVIIGFVFGTKMLGGLLAGV
                     TVSGVMMALFMANAGGSWDNAKKHIEGGSHGGKGSPAHKAAVVGDTVGDPFKDTAGPS
                     LNILMKLMSVVALVIAPMLVKTTPLLEKFLHR"
     gene            complement(9401..10093)
                     /locus_tag="PKI64_07945"
     CDS             complement(9401..10093)
                     /locus_tag="PKI64_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE00978.1"
                     /translation="METFDNYSESADTDRRHTVTVVAVEPLAGEAFLLRTTRGGYDFI
                     PGQNVSIALHLDYARSKDFSICSGQADDFLEFMIKGNRAGTITPLLRTLEPGAKLDLT
                     GPYGEFFYRADEKCRHVFLATGIGIGPFRSFLRSFTIPDYLVVHGVRRKADLALAAGI
                     DPTRLVTCVSREDGGTLRGRITDYLRNTELGVRDFYYLSGNPFAVKDVFDILSQRGVP
                     RERIVREFYYTY"
     gene            complement(10112..11171)
                     /locus_tag="PKI64_07950"
                     /pseudo
     CDS             complement(10112..11171)
                     /locus_tag="PKI64_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
BASE COUNT         2044 a         2856 c         4041 g         2230 t
ORIGIN      
        1 ttgatgcgtc cggcgggcca ggtggcgggg atggtgaggg cgaggcggta ggagctgggg
       61 gagaccggcg tgaccgcgac ggtaagattg ggatgggtga cctcgatgga gagaagttgg
      121 aagggggtct gattttcgga atggagcgaa agagttcggg taatggattg cccttggggg
      181 gcggagaaaa gtaggacggc ggagggggtc aacgtgaggt attggacggc ggttccctgg
      241 atgtgggcct gcctggaggg ggcatccgga tcgttggagg cgatggtgac gcgctggtgt
      301 tgggggccgc tgcggtttct gagggaaagg gtggcggtta gcggggtttc ttctccggga
      361 gcaagttcgc gtttgtccag gcgagaggtg gtgcaaccgc aactggtgcg gacatggttg
      421 atgtggaggg gttcatcgcc agcgttgcgg agggtaaagg tggcctgaac ggattcaccg
      481 gagggtacct cgccgaaatc gaacgtctcg ggattgatga tcagttttgg ggcggcagag
      541 agcggttgga gcagagtcaa gagggtaaac aaagcgtagc cagttgtgcg cagggaggtc
      601 atggggcagc ctgtcgttgc ttgagttggg tgcagaggcg ctggaagtcg gcaatggcgg
      661 gttgaacgag gtggggggcc tcggtgagga tgttcatttc gatgctgccg taggtgccgg
      721 aaagggagag gttggagctg ccgagcaaaa ggattcgtcc gtcgatgatg acgagttttt
      781 tatggagggc tttgttggtg ccgctccaga ggagggtttg gatgccgtcg ccggagagtt
      841 gggcggcgaa attggcattg gcgcggcgca tggcgggcgg cgaggcggtg ctattttcga
      901 cgaggatgac aacctcgacg ccggcagcgg ctttttcgcg gagggtagcg tagaaatctt
      961 tgatgggagc ggcgcggctg tcgctgaagt agtaggcgga aatccagatg gagttagtgg
     1021 ccgaacgggc ggcttcgagg agggctttgc cgaagggttc ggcggcgagg aggcgaactt
     1081 cggcgggggc gggggctttg gagagatttc gggcgacgga gtcgaggccg aggagtttgg
     1141 cggcctgggc tggatcggca tagaggacgt aagcagtggc ttcgcgggga atgcgctgga
     1201 tttgggtggg ggcccccgcc gggatgagaa catatccggc ggaggtttcc tgaatgaggc
     1261 gaccggaaag ctcgcgtccg ttgcggagga gaatcaggtc ggaagggttg gtttgggcta
     1321 ggccgaaggt cgcccgagcc aacaagagga gtgaaaggag ggaatagcgg aggctcacgg
     1381 catttcctcc ggttcgagct gatcgaggag ggtgtcgcgt tccttgccgg aagtgtttcc
     1441 cagttgccgg agggtgcgca actcctcctg ccaaagcgca ggggtgcgca tgtcgcggat
     1501 ggattcgcgg tttaggatat ggtcggccag ccgccgatcc tgttggggca ggacgagcag
     1561 gacgccggat tcagtgactt tttggctgga gagggcgcct tcggaccaat tgtgggagcc
     1621 gatcaggact ttggcaaggt ccacgacaac gactttttca tgcaggggga tggtggggat
     1681 atgcaagcgg acatccacgc ctttggcgcg gagattttcg gcccagtcca gatggatttg
     1741 gttcatggtg acggcttcgg acaggggcat gtggaggacc agatggacat agactccgcg
     1801 ttgaacagcc tgcgccagag cggcggtgag ttggttgagg ttgggttggt ccttttgggg
     1861 ctcgcccatg acatacatgg aggcaaggac cgattgatgg gcatttcgga tcaggtccag
     1921 caaggcgggg aggtattctt cgtcggcgag atagcgggcg gaggcggaaa gaaggaggtc
     1981 ttcgctggcg acgtcttgaa ggccagacgg ccagttggtg cggggaccgg tgggtggcaa
     2041 gccgatgggg aattcggcgg aggccggggc attggatggt gctgaaaccg aaagagaagc
     2101 gggttggaaa ggggttgcgg cggcatggag ggtgtcgaag tgccgggtat aggcgcgcgc
     2161 cagatcggcg gactcaatga gaaccaaatc ctcgatgttg tgcgcgaagg cggattgaga
     2221 ccagttggcg gagccggtcc agacctgcgc ttcgtccagg acaagcgtct taagatgcat
     2281 actgaacttc tcgttgccca gccaattcac ctgcaccccg gcggccacga gtgtttccag
     2341 cacggttcgc gaagaagagt cgagcattcc ggaatcgaca agcaggcgaa cgggcacttt
     2401 gccctcggtt gctttttggg cgagggcctc gacaagcgta ggttcaacca gcagatacat
     2461 ggcgacatcc acccgcttct gggcggcgtg aatgcgggat agaatggttt cggatgccaa
     2521 caatccgttg tcgggttcgg aatcagagga gaagaagacg cgggcggatc ggacggagct
     2581 gaagcgctgg agccgcgggg cggcatatgt cagcgtcggg ggtttacttt gggaaagctt
     2641 gctgggcttg gactcgaagc gttcggagtc gtcaaggcgg ggggcatgat aaagttcgga
     2701 aattggcagc aaacgatcca tacgcgcacg ataggcttga gcaagggccg gggattgcag
     2761 aaagagagaa tcctcgacat tgttttcgaa tccgcgaacc gtccaattgg ctgctccggt
     2821 gatgacaacg ccgtcgtcca cgacggcgca cttgaggtgc attttgccgt cgggcacttg
     2881 gaccacgtag acctcgacgc cgttttgggc aagccggtcc agaacatgac attcagcggg
     2941 agcggtggag gcggggtcca tgaagcagcg gaccttgagc ttttgcttgg ccgacaggaa
     3001 gcagagggtg tcgatcagat tggtatggct taggaaaaat ggcgcgacat ctatgcgccg
     3061 cttggcggaa tggagcgttc ggataatttc agaaagagaa tccccctgag gggagaaggt
     3121 gaccgagacg ccgcggacat ccagagccgt cgcggcaaga gaagaaattg gaagaagcag
     3181 ggaggtgatt aggaaagaaa catgccgcgc tatacggctt gcgagatgcc gagatgccga
     3241 gatgccgaga tgccgagatg ccgagatgcc gagatgccca aaatcatgca atagttgtgg
     3301 gcaaaagaat ttgattttgc aagataatca acattaggat ttcgttaaaa taggagataa
     3361 ttgttttgca aagattgttc ataaatccgt cgggctgtag aaaaatacat cagcggcgac
     3421 ttggggcttt tcttggttgt agaatcccaa aacgcttgcg ttgggcgcgg gcggcaagga
     3481 tgatgcggga caaatggcgg agcgtctcct agaagtgaaa gatttgcgtg cggggttcgc
     3541 gacgccggaa ggttttgtgc gggccgtgga tggggtgtcg tttgagattc gccgtggcga
     3601 agtgctgggg ctggtgggag aatcgggctg cggcaaaacg gtgacggcgc tgacattgct
     3661 gcggctgctg ccgcgcccgc cggcggtgat cgaaggcggg caggcgttgt tccacgggcg
     3721 ggatttgctg acgatgccca ccgaagaatt gcggacgatc cgtggcggta aaatcgggat
     3781 gatttttcag gagccgatga cggcgctctc gccattgcac cgcatcggcg accagttggc
     3841 ggaaaccgcg cggctgcacc gcaggctatc cgcggcggag gcgtgggcgc tggggctgga
     3901 gtggctcaag cgcgtgggcc tgccggatgc cgcgcagcgg atgaaggatt atccgcacca
     3961 gctttccggc gggatgcgcc agcgggtgat gttggcgatg acgatgttgc cggagccgga
     4021 gttgatcatt gcggatgagc cgaccaccgc gctggatgtg acgatccaag accagatttt
     4081 tgatttgttg ctgaagttgc gcggtggcga gacggcggtg ctgctgatta cgcacgacat
     4141 gggcgtggtg tgggaggtgt gcgaccgggt gcacgtgatg tatgccgcgc ggctggcgga
     4201 aaagggcacg cgtgagcaac tgttttcgac gtcggcgcat ccgtacacgc gggggctgtt
     4261 ggcggccatg ccgcgcttgc atggccgagc ggagcggttg ccagatattc ccggccagtt
     4321 gccgtcgctg cggaatccgc cgccgggctg ccggtttgcg ccgcgttgtc cgctggcgcg
     4381 cgcggtgtgc cggcagcacg cgccggcact gcgcgagttg ggcggggggc atgaagtggc
     4441 ctgctgggat ggcatttggg atcaagcgcc gggagcggaa catggctagc cgcgcgcctc
     4501 gtttcagttt ggtgaacctt ggttgcccaa agaatacagt ggattccgag gggattctcg
     4561 gcagcatggt tttggcggga tttcagttgg tggatgatcc ggcgacggcc gacatctgcc
     4621 tggtgaacac gtgcggattt ttggcggcgt cgcggcagga ggccgcgggg gtgttggcgg
     4681 aattggcagc gcagcgcggt cgccggcgcc gcccgctgct ggtggcgttg gggtgcatgg
     4741 tggagcgggc ggggggggcg ccgacgctgg cagacgggtt ggcggcagca gacgcgcggg
     4801 tgggctttgc cgattatgtg aagttgccag agatttgccg caacctgctg gcggggaaga
     4861 gagcagcagc gcgcgcccgc ggttacaccg ggcgcacgtt gcccaagacg tatctggcgt
     4921 ggttggatcg tccgcgcctg cgcatcggct ccgtggccgc ggcttacttg aaggtgggcg
     4981 aaggttgctc gaattgctgc gcgtattgtt cgattccgct gatccgcggc cggcgggcca
     5041 gccggccatt ggccgcggtg gtgaaagagg cgcgagaact ggtggccagc ggcgcgaagg
     5101 aactgaacct gattgcgcaa gacaccacgg cctatggcgt ggattgggcg ggccggccgc
     5161 aactggcgcg gctgttgcgt gagctgctgg ccatgccgga cgatgtgtgg ttccgcattc
     5221 tgtatgcgca ccccaagcat ctctcggcgg aggttctcgc ggtgatggcc ggcgacgcgc
     5281 gggtgtgccc ttatttggat ttaccgttgc agcacgtcca tgaccggatg ttgcgacgga
     5341 tggcgcgtgg ttatgaccgc gggcgcgtgg atgatgtgtt gcggatgatc ggccacgcgc
     5401 tgcccaacgc ggcgctgcgg accacgttca ttgtcggcca tccgggcgaa ggcgatgccg
     5461 agttttgcga gctgctggag tttgtggagc aggggcattt tgatcatgtg ggcgtatttt
     5521 gctggtcgcc ggaaccgggc accgcctcca cggccatgcg cgagcgcgtg ccgccggagg
     5581 tagccgaaga acgccgcgca gctttgctaa ccgcgcaggc cgcggtttcc gcccgacgcc
     5641 tgcgggcgcg gctgggacgg gaaacgcgcc tgctactgga ggaaaaaatc aaacgcggct
     5701 ggcgcgggcg tacaccatgg caggcgccgg aggtggatgg ccacaccacg ttggttggaa
     5761 gccggcgcgg gttgcaggcc ggtgattttg tgacggccca aattgtgcac accgatgtct
     5821 atgattgccg cgcgcggcag gtgatgccgt gaaccgggtc agttgttggc gccgccgggg
     5881 agcggtgatg ggtctgcttt gcctgctggc tgttgcagcg cgcgcggagg aaatcgccga
     5941 taccacaccc cccgcaatag caaagccgga acggtcgcgc atcctgcggt tgaacctcga
     6001 agaaatttac tatttgcagg gcttggaact ggaggagcaa tccagccacg agcatgccgt
     6061 aatcacccgc gagaacaacg tgacctcgtg gctggaccgg cagcatggcc ggctattccg
     6121 ctggctggac aaccgcgtgc gctgggtgga catggcttgg ctgggcgaaa agcgggccta
     6181 tgatccggag ctttccacct tccggctttc cacgatgacg cgcgtgggcg ggcgcagcag
     6241 cgaaaaggaa tttgacatca aggtccgctt ccgcggcgat ttcgcattgc cggcgttgtc
     6301 ccaccggctg cacgtatttc tggacaacgt ggggcgcgaa tcgctgcccg gtaaagaccc
     6361 catgcaacaa gactccaata cgcggctggg gattcgcgcg atgctcaaga ccctgaagaa
     6421 cagcgagcta tccgccaatg gcggtttgcg ctggcggcgc tcgcatccgg tggcttatgc
     6481 gggagtggat tggcgctacg cgcggccggt gggcgacgcg gtgtacgctt ttacgccccg
     6541 cgtggtgtac tacaccgatg atgggtttgc ccagcaggcg atcatgacgt ggacgcggca
     6601 ggtcggcgag cgcaagattt tccaggtgcg gctgggtgag cggtcggcgg agaacatgga
     6661 cggcgtggag ttcgagcaaa gcctgcgctt tgcgtggttg aggagcgggc gggggcgcgg
     6721 gtgggtggcg caggcgagcg tgttccccca tctggaaagc tctgactggt attgggatga
     6781 ctcgttgatc aatgtggctt ggcgcgatgc gctgtatcgc aagtggatct attacactat
     6841 tattccgcag cttcagttcc cgaaggaaga ccagtatgag ccgcgaccgt cgttgcggat
     6901 cgggctggac attctgttcg gggggcgcat cggcgaactg ttgtcccatt gagtctgggc
     6961 ccgcgggggg ggggcggaaa tcatgcgatt ttggcgggtt catgtttgac agggggcggt
     7021 gaatccccta ggctcccgac gttcgatagt tagaaaagaa acaaggagaa acgttttact
     7081 atgaacgaaa gcacacttct ttttgcggct ttgggttcgg cggtattggc gttggcgttc
     7141 gcttggtggc gtacgcaatg gatcaacaaa caagatccag gcacggcacg gatgcaggaa
     7201 atcggcgcgg cggttcgcga aggcgccatg gcttttctgc ggcgtgaata caaggtgctg
     7261 gccattttcg tcattgtggt ggcgggcctg ctggcctacc tgaacgtcaa ggtgacgggg
     7321 cagaagaatc tgtcgctggt ggcgctatcg tttgtggtcg gcgcgctttg ttcggtgctg
     7381 tccggctatt tcggaatgcg cgtggccacg gcggccaata tgcgcaccac gaacgcggcc
     7441 cgcaagggtc tgcccgaggc cttggccgtg gcgtttgccg gcggctcggt catgggaatg
     7501 tgcgtggtgg gcctgggcct gctgggcctg accggcctga cgctggtgta tttcaagatg
     7561 tttggcaccg aaaatgagat gctcaatggc acggtgctgc cgattctgac cggcttctcg
     7621 atgggcgcca gctcgattgc gctgttcgcg cgcgtgggcg gcggcatcta taccaaggcg
     7681 gcggatgtcg gcgcggactt ggtaggcaaa gtcgaagcgg gcatccctga agacgatccg
     7741 cgcaacccgg ccgtgatcgc cgacaacgtg ggcgacaacg tcggcgatgt ggccggcatg
     7801 ggcgccgact tgttcgagtc ctatgtgggt gccatcgtgg cggccatggt gctggcggcg
     7861 gtgtcctcca ttccggcggg cgccaagggt tcggaaatga atctggtgat gctgccgctg
     7921 ctgttggcgg gcgtgggcat tctgttttcg attctaggca cattcatggt gcgcacccgc
     7981 gaaggcggca acccgcaaaa cgcgctgaac gcgggctcgc tgagcgcggc ggtgctgacg
     8041 gccgcagcgt cgtatgtggt gattgccaag tttgcgccgt cggcctttag cgtggccgcg
     8101 ctaccgggca tggcggccaa ggtgtacaac gccaatggca ttttttgggc cacgctggcc
     8161 ggtttggctt cgggcgtggc gattggcttc ctgtcggaat actacaccgc ggaaggcaag
     8221 ggcccctcga accgcattgc ggatgcttcc tccaccggcg cggcgaccaa tatcatcgcc
     8281 ggcctcggca acggcatgat gtccaccgcg ctgccggtga ttgtgctggc ggcggccacc
     8341 atgatttcct acatgacggc gggcctctac ggcatcgcca tcgccggcct cggcatgctg
     8401 gccaccaccg ggattcaact ggcggtggat gcttatgggc ccatcgcgga taacgcgggc
     8461 ggcctcgccg aaatggcgca cctcgaaaag gaagtgcgcc agcgtaccga caagctggat
     8521 gcctgcggca acacgaccgc ggccatcggc aagggttttg ccatcggttc cgctgcgctg
     8581 acggccctcg cgttgtttgc ggctttcggc cagaccgtca agctcgacaa gattgacgtg
     8641 atggatccga acgtgaccgc cggcctgttg atcggcgcga tgatgccgtt cctgttctcc
     8701 gcgttcgcca tgcaagcggt gggtcaggcc gcgtttgcca tggtggaaga agtccgccgc
     8761 cagttcaagg agatccccgg cctgctggaa ggcaaggaag gtgcgcgcgc cgactacgcg
     8821 cgttgcgtgg atatttccac cgccgccgcc atcaagaaga tgacggtgcc ggcgctgttg
     8881 ggcgtgttga cgccggtaat catcggcttt gtgttcggca ccaagatgct cggcgggttg
     8941 ctggcgggcg tgacggtgtc gggcgtgatg atggccctgt tcatggccaa cgcgggtggc
     9001 tcgtgggata acgccaagaa gcacatcgaa ggcggttcac atggcggcaa gggcagcccg
     9061 gcgcacaagg cggccgtggt gggcgacacc gtgggtgacc ccttcaagga caccgccggg
     9121 ccgtcgctga acatcctgat gaagctgatg agcgtggtgg cgttggtgat tgcgccgatg
     9181 ctggtcaaga ccacgccgct gctggagaaa ttcctccatc gctaaccgcc ggtcggcgat
     9241 cggcccccgc gcttgccgcg ggggcttttt cattggctag agcattttca gcaatgcgag
     9301 aaggttgcgt tcctcaacca ttcccgcccg acaaaagcag ccggttggat tgtggggctg
     9361 gaacggtcgc agacctgcca gccttagttc ttctgatgct ctaatacgtg tagtagaact
     9421 cgcgcacgat gcgctcgcgt ggcacgccgc gctgactcag gatgtcgaac acatccttga
     9481 cggcgaacgg attgccgctg aggtagtaga aatcacgcac gccaagctcg gtgttgcgca
     9541 gataatcggt aatgcggccg cggagggtgc cgccatcctc gcgcgagacg caggtgacca
     9601 gccgcgtggg atcaatcccg gcggccagcg ccaaatcggc cttgcggcga accccgtgca
     9661 ccacgagata gtctgggatg gtaaagctgc gcaggaatga gcggaagggg ccgatgccaa
     9721 tcccggtggc cagaaacacg tgccggcatt tttcgtcggc gcggtagaaa aactcgccgt
     9781 aggggccggt caagtcgagc ttggcgccgg gttcaagagt ccgcaatagc ggcgtgatgg
     9841 tccccgcgcg gttgcccttg atcatgaact ccaaaaaatc atccgcctgc cccgagcaga
     9901 tggaaaaatc cttgctgcgc gcatagtcca gatgcagcgc gatgctaaca ttttggccgg
     9961 ggatgaagtc gtagccgccg cgcgtggtgc gcagcaggaa agcctcgccg gcgagcggct
    10021 ccaccgccac cacggtgacg gtatggcggc ggtcggtatc ggcgctttcg ctgtagttgt
    10081 cgaaagtttc cacttcgccg gtgtgctcgg gttcgcgcag atcgccttgc cgcgggtctt
    10141 ggccgacatc gaagggcagt ggcaacttgc cgcgttcgat cttgcggccg gccatactgc
    10201 gcagcttgtt tttgagcacc cacgtggcgg gatggcgcag atattttttc atgacgggcg
    10261 cggcggtgcc gaccatccag caattcttgg ggcaggtgcg gaccaacgac cgcactttgt
    10321 cggcctgcgg gccataccaa atgtcttcga acgatttgac ttgccggatg ttgccgaaac
    10381 tttccttcca gtagcgctct tccagcccgt tgcacggata gacatcgccg taaggttcga
    10441 tgaagaaatt gacggtgcct gcctcgcagg gcaggagccg ccggttgccc ttgatgtagt
    10501 tgatcaaccc cagattgaaa aacgcccgaa accagctctt gggattccgt tcttccagca
    10561 gccgatcaat caactcgtag aaattgttgc agacttcgtc ctggttggtg atgacgttgt
    10621 cgtctttgtg gaagtagtac gagttatggt aggcggcggt ggcgaactcc atcttgagat
    10681 ttttggacag ctcatacaac tcgagcatat cggcgctgtt gttgttggag accgtgatgc
    10741 cgaaaccgat gtccttgatg cccaaccggc gcaagcccaa cagcgttttg agcccgcggt
    10801 caaagccgct gtcgcgcccg cgcaggtagt cattgatggt ggagaggccc tcgatgctga
    10861 cccggatgcc aatgcgcgga aacttttccg ccagggcgag gatttcatcc acctgatagc
    10921 ccgaggtgga aatcaccacg cgtggcgctt tgcggaagct gacctccacg atttcgtcca
    10981 gatcgcgccg gacgaacggc tcgccgccgg tgatgttgac gaacttcaac tgtggcagaa
    11041 tctccaattc ttccgcgcgg atttcttggg cgatttcggt cgggtagcgc cagatgttgc
    11101 acatcttgca cttcatgcag cagcggtaag tggtgatgat ggagacatcc gtggggaggg
    11161 gggcgcgggg g
//
LOCUS       DAOPNP010000082        11167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6696_length_11167_cov_9.126080, whole genome shotgun sequence.
ACCESSION   DAOPNP010000082 DAOPNP010000000
VERSION     DAOPNP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_11167_cov_9.126080"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1539
                     /locus_tag="PKI64_07955"
     CDS             157..1539
                     /locus_tag="PKI64_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00979.1"
                     /translation="MEELTLDQIPRKTRDLYEKAMAALERGNTGYALDMLKQVITVEP
                     RFLLARKNLRIAQVKLAVAAKPNAMTHQLSSLKGAFTLMAAQGKLKKDPKAALDGAEK
                     LLAMDPLNLSFLKFFAQAAEAAEMTELAVQTLEIARPYYTRNVDFLRLLAKLYIDTNQ
                     PGGAKDCYAAVVELRPNDQQAIKNLKDAAALDTMKAGNWTDMKSDFRSKLKDQKEAIR
                     LEQQAKSVKGESDIDALIEDRLRDIQREPQNMNFRRALADLYVRAERFDEALQALADA
                     TEAAGRSDPQIERTVSQIKARQFDNAIAAAKAANDEAAVAAQEAAKAQFLYDDAVEMV
                     KRYPNDLQFRYELGYQYYLRDQFNEAIEEFQLAQRNPQRRTRALYYLALCFTAKGQYD
                     IAFEQLQKAASELTLMDDTKKDVVYEMGVLAEKMGRAQEATGYFKEIYAVDIKYRDIA
                     QRIEAAYQKK"
     gene            1548..2573
                     /locus_tag="PKI64_07960"
     CDS             1548..2573
                     /locus_tag="PKI64_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE00980.1"
                     /translation="MKRTALLLAAGWLLAVVFIGSGCGRSTCAPAPAVAESAAPAGFP
                     RAVAGEYSVMTFNLHQYALLDRDGNSDTLEPKPREETEAIINAIQEAAPDILAVQEMG
                     DPAAWAEFKYNLRQAGLDYPHEEYLRRGKSDLNIAVLSRFPIVERQSHTSDTYTIGPA
                     QFPILRGVIDITIEINPDYRLRLMVAHLKSKVFHSFGQAEMRRSEARLLNNHVRDALR
                     ENPEVNLLVVGDFNDDPSSAAVREIRTYKRETLLHDLRPTDAVGDAWTHRQNDDVYHR
                     IDFMLASRGLLPEVLPDKCYAVRAPGLERASDHRPIVATFAATDRGAESAPDLSARIP
                     PEIPQND"
     gene            2594..3451
                     /gene="purU"
                     /locus_tag="PKI64_07965"
     CDS             2594..3451
                     /gene="purU"
                     /locus_tag="PKI64_07965"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOE00981.1"
                     /translation="MPSPTAILLVSCPDQRGIVAALSSFITQHNGNIIALDQYVDNQE
                     GVFFARMEWELAGFGCPLDDFTRCFAPVADPLHMTWRLFRSDKPHRMAVMVSKQAHCL
                     YDILARYESGEWPAELVLVLSNHEDHRGLVERHGLPFHHIPVTAATKAAAEAQQIALL
                     REAGVDLVVLARYMQILSPAFVDAFPMRIINIHHSFLPAFAGANPYRAAHERGVKIIG
                     ATAHYVTAALDEGPIIEQDVHRVSHRQGVDDLVRLGRDLEKIVLSRAIYWHLHHRLLV
                     YKNRTVVFS"
     gene            3451..3948
                     /locus_tag="PKI64_07970"
     CDS             3451..3948
                     /locus_tag="PKI64_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00982.1"
                     /translation="MPAPAAPLPQRRAECVAELLRALAACATAARATPPPWPAPEPRL
                     PAPPLADIFYALAPAGAADPVEAHRALYRAFTAYQQLICAEAEAKAAQHPADFQTALA
                     AGDREQIALLLTDLGGELQMLDHVRTVTPNIAPALPTDVALFLWREHLLPWSRAVALA
                     HNCEP"
     gene            4025..4555
                     /locus_tag="PKI64_07975"
     CDS             4025..4555
                     /locus_tag="PKI64_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00983.1"
                     /translation="MNLKRQDILVTLKLLVRGGLDCTFAQLGRELGISASEANQAFHR
                     ARQAGLIHPLEDVAIESSVAELLIHGLKYVFPVQPGRRTRGMPTGFAAPPLKQFFKVV
                     RDDPNLPVWPDPEGEYAGVEIKPMSRSVPDAARRDPKLYEWLVLADALRGAGRARERE
                     LAEKIVHEKLGGHAPH"
     gene            4518..5240
                     /locus_tag="PKI64_07980"
     CDS             4518..5240
                     /locus_tag="PKI64_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00984.1"
                     /translation="MSTKNSVVMHPIDFHSLAAIARQLDLLQTPYAFTGGAIVGFLLD
                     HPHLASLRPTADVDVIVAVITHLQHAALEERLRKLGFHHDTSAGAPICRWRYLGTRVD
                     IMPAQDTAGQKSNPWFAHALRGASLKTWDQVTARIVSATSFVATKLVAFADRGRNDFF
                     GSHDLEDVVTIVDGRAAFVDELAAEEPTMRNYVGCEIRRLLGLPKFIDSLPGHLASDE
                     ASQQRLPILLSRLQAIADLCAP"
     gene            complement(5262..6887)
                     /gene="pgi"
                     /locus_tag="PKI64_07985"
     CDS             complement(5262..6887)
                     /gene="pgi"
                     /locus_tag="PKI64_07985"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOE00985.1"
                     /translation="MPRRKRLDISPEWKALKAHAAQMKTASLRELFAADPARGTTFSL
                     DVGGWHLDYSKNLITNETMRLLQALCAAADLRGEIDAMFAGKKINQTENRPVLHIALR
                     NRANTPIYVDRKDVMPAVNAVLAKMSDFAARVRAGEWRGFTGKPIRNIVNIGIGGSDL
                     GPVMAYEALKPYSRRDLTVRFVSNVDGTHIVETVRDLDAAETLFIVASKTFTTQETMT
                     NAGTARQWLLERLGDPAAVARHFVAVSTAAQEVQAFGIDPANMFEFWDWVGGRYSLTS
                     AIGLPLMIAIGPENFVAMLEGYHAMDRHFATAPFTANMPVILALLGIWYGNFFGAQST
                     ALLPYDQYLSRFAAYFQQGDMESNGKRVTKNGKIVAYETGPVVWGEPGTNGQHAFYQL
                     IHQGTKLIPCDFIGFCRSHNPVGDHHVKLMSNFFAQTEALAFGKTAEECRAEGVPEKL
                     VPHKTFSGNRPTNTLLADQLTPETFGQLVALYEHKIFVQGVIWNIFSFDQWGVQLGKV
                     LANRILPELQDKSAPLQHDSSTNELIRRFRERA"
     gene            complement(7067..8284)
                     /locus_tag="PKI64_07990"
     CDS             complement(7067..8284)
                     /locus_tag="PKI64_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE00986.1"
                     /translation="MAKKNKKITGNMLIAQSGGPTVVINQSLIGAVLEARKHKEIKKI
                     YGALHGIKGILNEDLIDLGRESVATLKAVAQTPSSALGSVRKKPTPDDCAKIFEKLQK
                     YGVRYFFYIGGNDSAETAHIINEEAKKAGYELHCFHIPKTIDNDLRENDHTPGFASGA
                     KFVAQAFMGDDLDNAALPGVKINVVMGRHAGFLTAAAALARTYPGAGPHLIYLPERPF
                     DLELFPQQVKAMVEKHGRCVVAVSEGIADITGTAIAATFTKEVDSHGNVQLSGSGALG
                     DALANLIKTKTDITRVRADTFGYLQRSFPGVVAANDAKEAFAVGRAAVKLATSGDVDG
                     SVAIRRKKGKKYQVYFERVELRSVAKETRHMPNEFIAANGCDVTKAFIDYAAPIVGPL
                     PKIGRFKRIKVKK"
     gene            8466..8735
                     /gene="rpsO"
                     /locus_tag="PKI64_07995"
     CDS             8466..8735
                     /gene="rpsO"
                     /locus_tag="PKI64_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005507098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOE00987.1"
                     /translation="MMMDSAVKAAIKQEYQQHEKDSGSVEVQVALLSKRIQDLTAHLQ
                     SHKADHNSRRGLIVMVSKRRRLLDYLRDTDLARYQKLIARLGLRR"
     gene            8935..11052
                     /locus_tag="PKI64_08000"
     CDS             8935..11052
                     /locus_tag="PKI64_08000"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOE00988.1"
                     /translation="MKNTTSVAVNLGGKTMTFETGLLARQAAGQATCQLGETMVFSAV
                     TSTSKPREGIDFFPLQVEYRERFYAAGRFPGGYFKREGRPTEKEILTARVTDRPIRPL
                     FAEGFFNDVQINNALMTADGEHESDILSINAASAALMLSELPFLGPIGAVRVGRVDGQ
                     FILNPTQQQLKASDLDLVYVGRRDLPMMIEGSAKEISNADMVAAMKFGHEGVVAICDA
                     QLELRAKMGLPPKTFTETGPDAEQVAKLREIAGADLEAALFTVKKLERQEKMGKVFEA
                     AFAKMQEIYGEEVMTENRFKMLFDVIEIEIVRKGVLEKGKRMDGRAMDELRPLIADVA
                     LLPRVHGSALFSRGETQAIAVATLGTGQDEQEMDGLTGGDTKKRFTLHYNFPPYSTGE
                     VKRLGGLNRREIGHGALAERSIAQMMPADYPYTVRVVSEIMGSNGSSSMASVCVGTLA
                     LMDAGVPLKKPVAGISIGLVTGASQKPVLLTDILGAEDHCGDMDFKVAGTRDGITGFQ
                     VDLKIRGLEWDLVAGAFDRAHAARLKILDTMQAALPAPRPELSPHAPRIMVVHINPEK
                     IGALIGPGGKNIRRITETTGTQIDIEDDGKVSIFSNNKDNLDAALREVEALTAEAEVG
                     KIYRGAVTGIKDFGAFVEFLPGQEGLVHISELADFRVNRTEDVVKMGEEIWVKVLSVE
                     DNGKVRLSRKAAMAEKGEKSRKG"
BASE COUNT         2182 a         3534 c         3344 g         2107 t
ORIGIN      
        1 aaacggggcc ccacgcagag aaatatttcg gtgtcatgaa ttgtgaggca acgcgagcga
       61 ttcgcggtta tgccgtttgc gattgacggg gtgttgcttt ccaacctatt gtctacggaa
      121 taccacctcg ttgttgcgtt ggtgaaggag tgcgatatgg aagagctgac attggaccaa
      181 attccccgca aaacccgtga tctttatgaa aaggcgatgg cggcgcttga gcgcggcaac
      241 accggctacg cgctggatat gctcaagcag gtcattaccg tcgagccgcg tttcctgctg
      301 gcgcgcaaga acctgcggat tgcccaggtc aagctcgccg tggcggccaa acccaatgcc
      361 atgacccacc agttgtcctc gctcaaaggc gcgttcaccc tgatggccgc gcagggcaag
      421 ctcaagaagg atcccaaggc ggcgctggat ggggcggaaa agctgctggc catggacccg
      481 ttgaatctgt cgttcctcaa gttttttgcg caggcggccg aggcggcgga gatgaccgag
      541 ctggcggtgc aaacgctgga aatcgcccgc ccttactaca ctcgcaacgt ggactttttg
      601 cgcctgttgg cgaagcttta cattgatacc aaccagccgg gcggcgccaa ggattgctat
      661 gccgcggttg tcgaattgcg ccccaacgac cagcaggcca tcaagaacct gaaggatgcc
      721 gcggcgcttg ataccatgaa ggccggcaac tggaccgaca tgaagagcga cttccgctcc
      781 aagctcaagg accagaagga ggctatccgc ctggaacagc aggccaagtc cgtcaagggc
      841 gagagcgata ttgacgcgct gatcgaggac cggctgcgcg acatccagcg cgagccgcaa
      901 aacatgaact tccgtcgcgc gctggccgat ctctacgtcc gcgccgagcg tttcgacgag
      961 gccctgcagg cgctggccga tgccaccgaa gccgccggcc gttccgatcc gcagatcgag
     1021 cgcacggttt cgcagatcaa ggcacgccag ttcgacaacg ccattgccgc cgccaaagcg
     1081 gccaacgatg aagcggctgt ggccgcgcag gaagccgcca aggcgcaatt cctgtatgac
     1141 gacgccgtcg agatggtcaa gcgctacccg aacgacctcc aattccgcta cgagctgggc
     1201 taccagtatt acctgcggga tcagttcaat gaggccatcg aggaattcca attggcacag
     1261 cgcaacccgc agcggcgcac ccgcgcgctg tattatctgg cgttgtgctt caccgccaag
     1321 ggacaatatg acatcgcgtt tgagcagctc cagaaggcgg catccgagct gacgttgatg
     1381 gacgacacca agaaagacgt ggtgtatgaa atgggcgtgc tggccgagaa gatgggccgc
     1441 gcgcaagagg ccaccggtta cttcaaggaa atctacgcgg tggatatcaa gtatcgcgac
     1501 attgcccagc ggatcgaagc cgcctaccaa aagaaatagg cgccgccatg aagcgtaccg
     1561 cattgttgtt ggcggccggc tggctgctgg ctgttgtgtt cattggttcc ggttgcggtc
     1621 gctcaacgtg cgcgcctgcg ccggctgttg ctgaatccgc cgccccagcc ggttttccgc
     1681 gcgcggtggc tggcgaatat tcagtaatga ccttcaacct gcaccagtac gccctgctgg
     1741 accgcgacgg caacagcgac actcttgaac ccaagccgcg cgaggaaacg gaagccatca
     1801 tcaacgccat tcaagaggcc gcaccggata ttctggcggt gcaggaaatg ggcgatcccg
     1861 ccgcctgggc cgagttcaaa tacaacctgc ggcaggccgg tttggattat ccccacgagg
     1921 aatatctgcg ccggggcaag tccgatctca acatcgccgt gctgtcgcgc ttccccatcg
     1981 tggagcggca atcgcacacc agcgatacct ataccatcgg ccccgcgcag tttcccattt
     2041 tgcgcggcgt gattgacatc accatcgaaa tcaatccgga ttaccgcctg cggctgatgg
     2101 tggcacacct gaagtccaag gtcttccatt cgttcgggca ggccgaaatg cgccgtagcg
     2161 aagcccgttt gctgaacaac cacgtccgcg acgccctccg cgaaaacccg gaggtcaacc
     2221 tgctggtggt gggcgatttc aacgacgacc cctcctccgc cgccgtgcgc gaaatccgca
     2281 cctataaacg cgaaaccctg ctgcatgacc tgcgccccac cgatgccgta ggtgacgctt
     2341 ggacccaccg gcagaacgat gatgtctatc accgcattga tttcatgctg gccagccgcg
     2401 gcctgctgcc ggaagtactt cccgacaagt gctacgccgt gcgggcgccc ggcctcgaac
     2461 gcgcctcgga ccaccggccc attgtggcca ctttcgcggc cactgatcgc ggcgcggagt
     2521 ccgcccctga tctctcggcg cgcatcccgc ccgagattcc gcaaaacgat tagccgcccg
     2581 cccgctcaac gtcatgccct cccccactgc cattcttttg gtttcctgcc ctgaccaacg
     2641 cggcattgtc gcggcgttgt cgtcgttcat cacgcagcac aacggcaaca tcattgcgct
     2701 cgaccaatat gtggacaacc aggaaggcgt attcttcgcc cgcatggagt gggaattggc
     2761 cggctttggc tgcccgctgg atgacttcac acgatgtttc gcgccggttg ccgacccatt
     2821 gcacatgacg tggcggctgt tccgctccga taagccccac cgcatggccg tcatggtgtc
     2881 caagcaggcc cactgcctct atgacatcct ggcccgctac gaaagcggcg aatggcctgc
     2941 cgagctggtg ctggtgctct ccaaccacga ggaccatcgt ggtcttgtcg aacggcacgg
     3001 cttgccgttt caccatattc ccgtcacggc cgccaccaag gccgccgccg aggcccaaca
     3061 aatcgcgttg ctgcgggagg ctggggtgga cctggtggtc ttggcgcgct acatgcaaat
     3121 cctgagcccg gcgtttgtgg atgctttccc catgcggatc atcaacatcc accactcctt
     3181 cctgcccgcc tttgccggcg ccaaccccta tcgtgcggcc cacgagcgcg gggtcaagat
     3241 cattggcgcc acggcgcact acgtcaccgc cgccctcgat gaaggaccca tcattgagca
     3301 ggatgtccac cgcgtctcgc accggcaggg cgtggatgat ctggtgcgcc ttggccgcga
     3361 ccttgaaaaa atcgtgctgt cccgcgccat ctactggcac ctgcaccacc gcctgctggt
     3421 gtataaaaac cgcacggtgg ttttctcctg atgccggccc ccgccgcccc cctgccccaa
     3481 cgccgcgcgg agtgtgtggc ggagttgctg cgcgcgctgg ctgcgtgtgc caccgccgcg
     3541 cgcgccaccc cacccccatg gcccgccccc gaaccgcgcc tgcccgcgcc gccgctggcc
     3601 gatatttttt acgcgctcgc acccgccggc gctgccgatc ccgtcgaggc gcaccgcgcc
     3661 ctgtaccgcg cgttcacggc ttaccagcaa ctcatctgcg ccgaggctga agccaaggcc
     3721 gcgcagcatc cagcagattt tcaaacggcg ctggccgcgg gcgaccgcga acaaatcgcg
     3781 ctgctgctga ccgatctggg cggtgagtta cagatgctgg accacgtacg taccgtcacg
     3841 ccgaacatcg cgcccgcact ccccacggac gtggcgctgt ttttgtggcg ggagcatctc
     3901 ctgccctggt cccgtgccgt cgcgctggcc cacaactgcg agccctgaag cgggcgaaaa
     3961 gaaaaacgcc agcgaccggc cagttggggt tgacatattc gcaattcgcg aatagcgtat
     4021 ttatatgaac ctaaagagac aagatatatt agtcaccctg aaacttctgg tgcggggagg
     4081 cttggattgc acgtttgcgc aattggggcg ggaacttggg atcagtgcat ccgaggccaa
     4141 tcaggctttt catcgggcgc ggcaggccgg gctgattcat ccgctggaag atgtcgctat
     4201 tgaatcctcc gttgccgaac tgttgatcca cggattgaaa tatgtctttc cggttcaacc
     4261 cggccggcgc accagaggca tgcccaccgg ttttgccgcg ccgccgctga aacagttttt
     4321 taaagttgtg cgggacgacc ccaatttacc agtttggcca gacccggaag gcgagtatgc
     4381 gggcgtggaa atcaaaccca tgtctcgttc cgttcccgac gctgcccgcc gggatcccaa
     4441 gctatatgaa tggctcgttc tggcggatgc cctgcgcggc gcgggccggg cccgggagcg
     4501 ggaattggcg gagaagattg tccacgaaaa actcggtggt catgcacccc attgattttc
     4561 attctctcgc cgcgattgcc cggcagttgg atttgctcca aaccccatac gctttcaccg
     4621 gcggggccat tgtgggattc ctgctggatc atccccatct ggcctccctc agacccacgg
     4681 ccgatgtgga tgtgattgtt gccgtgatca cccacctcca gcatgccgcg ttggaagagc
     4741 gattgcggaa actgggcttc caccatgaca ccagcgcggg cgcgccgatt tgccgctggc
     4801 gttaccttgg cacgagggtg gatatcatgc cagcccagga tacagcaggg caaaagagca
     4861 acccgtggtt cgcgcacgct ctgcgcgggg ccagcctcaa gacatgggat caggtcacag
     4921 cgcgaatcgt gagcgccacc agctttgttg ccaccaagtt ggttgctttt gccgaccgtg
     4981 gcagaaacga cttctttggc agccatgatt tggaagatgt ggtgaccatc gtggacggac
     5041 gcgccgcatt cgtggacgag ttggccgcgg aagaacccac catgcgcaac tatgtgggct
     5101 gtgaaattcg gcgtttactg gggttgccca aattcattga ttccctgccg ggccatttgg
     5161 caagcgatga ggccagccaa caacgtttgc ccatactatt gagccgtctg caagctatcg
     5221 ccgacttgtg cgcgccttga cgcctgaatg gcttggaatg gctaggctct ttcgcggaag
     5281 cggcggatga gttcgttggt ggagctgtcg tgctggagcg gggcggattt gtcttgcagc
     5341 tcgggcagga tgcggttggc gaggaccttg cccaactgca cgccccactg atcgaacgaa
     5401 aagatattcc aaatcacgcc ctgcacgaag atcttgtgtt cataaagcgc aaccagttgg
     5461 ccgaaggttt cgggggtcag ttggtcggcc agcagggtgt tggtgggtcg gttgccggag
     5521 aaagttttgt gcggcacgag cttttcgggc acgccttcgg cgcggcattc ctcggcggtt
     5581 ttgccaaaag ccagcgcttc agtctgggca aagaagttgc tcatcagctt gacgtggtgg
     5641 tcgcccacgg gattgtggct gcggcagaag ccgatgaaat cacacgggat cagcttggtg
     5701 ccctgatgga tcaactgata gaacgcgtgc tggccgttgg tgccgggttc accccacacc
     5761 acgggcccgg tttcgtaggc cacgatcttg ccgtttttag tcacgcgctt gccgttgctt
     5821 tccatgtcgc cctgctggaa gtaggccgcg aagcgcgaga gatattggtc atagggcaac
     5881 agcgcggtgc tctgcgcgcc aaagaagttg ccataccaga tgcccagcag tgccaggatc
     5941 acgggcatgt tggccgtgaa gggcgcagtg gcgaaatggc ggtccatggc atggtagcct
     6001 tccagcatgg ccacaaagtt ttccgggccg atggcgatca tcagcggtaa accgatggcg
     6061 ctggtcagcg agtagcgtcc gccgacccaa tcccagaact cgaacatgtt ggccggatca
     6121 atgccgaacg cctgcacttc ttgggcggcg gtcgagaccg ccacaaagtg ccgcgccact
     6181 gccgccgggt cgcccaaacg ctccagcagc cattggcggg cggtgccggc gttggtcatg
     6241 gtttcctgcg tggtgaacgt cttggaggcc acgatgaaga gcgtttcggc cgcatccagg
     6301 tcacgcacgg tttcaacgat gtgcgtgcca tccacgttgg agacaaaacg caccgtcaga
     6361 tcgcgccggc tatagggctt gagcgcctca taggccatga cggggccgag gtcgctgcca
     6421 ccgatgccga tgttgacgat gttgcggatg ggcttgccgg tgaatccccg ccactcgccg
     6481 gcgcgcacac gcgcggcaaa atcgctcatc ttggccagca ccgcgttgac agcgggcatg
     6541 acatccttgc ggtccacata aatgggcgtg ttggcgcggt tgcgcagggc aatatgcagg
     6601 acggggcggt tctcggtttg attgatcttt ttgccggcga acatggcgtc aatttcgccg
     6661 cgcaaatcgg cggcggcgca aagcgcctgc agcagccgca tggtttcgtt ggtgatcagg
     6721 tttttggagt aatccaaatg ccacccgccc acatcgagac tgaaggtggt gccacgcgcc
     6781 ggatctgccg caaacagctc ccgcagcgaa gcggttttca tctgcgcggc atgcgccttg
     6841 agggctttcc attccggtga gatgtcgagt cgtttgcggc ggggcatggc aggtccttgg
     6901 gagtctagag gggctaggag tttaggagtc tagaagtcta ggagtttagg agtttaggga
     6961 gctagaaatt ccggggcgtg gcaaggggga gcataggagg gagagcgcga acatcggtta
     7021 tgctcctgca ctccctgact cctatactcc gccattcctc accactctac ttcttcacct
     7081 tgatccgctt gaagcggccg atcttgggca gggggcccac gatgggggcg gcgtagtcaa
     7141 tgaacgcctt ggtgacgtcg cacccgttgg cggcgatgaa ctcgttgggc atgtggcggg
     7201 tttccttggc cacgctgcgc agctccacgc gctcgaaata cacctggtac ttcttgccct
     7261 tcttgcggcg gatggccacg ctgccatcca catcgccgct ggtggccagc ttgaccgccg
     7321 cgcggcccac cgcgaacgcc tccttggcat cgttggcggc gaccacgccg gggaacgaac
     7381 gctgcaaata gccgaacgtg tcggcgcgca cgcgcgtgat atcagtcttg gtcttgatga
     7441 ggttggccag tgcatcgccc aacgcgccgg agccggaaag ctgtacgttg ccgtggctgt
     7501 ccacttcctt ggtgaaggtg gcggcaatgg ccgtgccggt gatgtcggca atgccttcgg
     7561 acaccgccac cacgcagcgg ccgtgctttt caaccatggc cttgacctgc tgcgggaaaa
     7621 gttcgagatc gaacggtcgc tcgggcagat agatcaggtg cgggccggcg ccgggatagg
     7681 tgcgggccag cgcggcggcg gcggtcagga agccggcatg gcggcccatg accacgttga
     7741 tcttcacgcc cggcagcgcg gcattgtcga gatcatcgcc catgaacgcc tgcgcgacga
     7801 acttggcgcc gctggcaaag ccgggggtgt ggtcgttttc gcgcagatca ttgtcaatgg
     7861 tcttgggaat atgaaagcaa tgcagctcgt agccggcctt cttagcttcc tcgttgatga
     7921 tgtgcgcggt ttcagcggaa tcgttgccgc cgatatagaa gaagtagcgc acgccgtact
     7981 tctggagctt ttcaaaaatc ttggcgcagt catcgggcgt gggctttttg cgtacggagc
     8041 cgagggcgga ggagggcgtc tgggccacgg ccttgagggt ggccacgctt tcgcggccca
     8101 gatcaatgag gtcttcgttg aggatcccct tgatgccgtg cagcgcacca taaatctttt
     8161 tgatttcctt gtgtttccgg gcttccagca ccgcgccaat caaactctgg ttgatgacga
     8221 ccgtcggccc gccgctctgg gcgatgagca tgttgcctgt gattttcttg tttttcttgg
     8281 ccataaaacg cttgatcctt tccggcgggg cgtccgccta cgcccgcgca tgaaaccaaa
     8341 ccggcgcccg tgctggcaag cggttttgcg gcccattttt ggccttgcat cgccccaccc
     8401 ccgcccgtat agtccgccct tcgaacaatc aagaccagag aagcagaaga gaagttggat
     8461 caagcattat gatggatagc gcagtcaaag cggcaatcaa gcaggaatat caacagcacg
     8521 aaaaggatag cggttccgtg gaggttcaag tggccctcct ctccaagcgg atccaagacc
     8581 tgaccgcgca ccttcagagc cacaaggccg accataactc gcgccggggc ctgatcgtca
     8641 tggtcagcaa acgccggcgc ctgctggatt acctgcgcga caccgatctc gcccgctacc
     8701 agaagctcat cgcccgcctc ggcctgcgcc gctagtcccg cgggggcgcc ccccttggcg
     8761 gggaggcccg cccctctcgc tggcgaccag gcacggccgt ggacggaccg ggctggcctc
     8821 ctttccttcc ttgcgccgcg tcggcatcgc cgcgaaacgc cggcttatgc cccgcgctca
     8881 aacgtcgaaa aagcagtcag taagtaagaa gaagaaaaga aggaaagaag caagatgaag
     8941 aacaccacct ccgtcgccgt gaatctcggc ggcaaaacca tgacattcga aacgggcctg
     9001 ctcgcccgcc aggccgccgg ccaagccact tgccaactcg gcgagaccat ggtcttttcg
     9061 gccgtcacct ccaccagcaa gccccgcgaa ggcattgatt tcttccccct gcaagtggaa
     9121 taccgcgagc ggttctatgc cgccggccgt ttccccggcg ggtatttcaa gcgcgaaggc
     9181 cgccccaccg aaaaggaaat cctgaccgcc cgcgtcaccg atcgccccat ccgcccgctc
     9241 ttcgccgagg gcttcttcaa tgatgtccaa atcaacaacg cgctgatgac cgccgacggc
     9301 gaacacgaaa gcgacatcct cagcatcaac gccgcttccg ccgccctgat gctcagcgag
     9361 ctgcccttcc tcggccccat cggcgccgtg cgcgtgggcc gcgtggatgg ccaattcatc
     9421 ctcaacccca cccagcaaca gctcaaggct tccgacttgg acctcgtcta tgtcggccgc
     9481 cgcgacctgc ccatgatgat cgaaggctcc gccaaggaaa tcagcaacgc cgacatggtc
     9541 gccgccatga agttcggcca cgagggcgtg gtcgccattt gcgatgccca gcttgagctg
     9601 cgcgccaaga tgggcctgcc gcccaagacc ttcaccgaaa ctggccccga tgccgaacaa
     9661 gtcgccaagt tgcgcgaaat tgccggcgcc gatctcgaag ccgcgctctt caccgtcaag
     9721 aagctcgaac gccaggaaaa gatgggcaag gtgttcgaag ccgccttcgc caagatgcag
     9781 gaaatctacg gcgaagaagt catgaccgaa aaccggttca agatgctctt cgatgtcatc
     9841 gagattgaaa tcgtccgcaa gggcgtgctg gaaaaaggca agcgcatgga cggtcgcgcc
     9901 atggacgaat tgcgtccgct aattgccgat gtggccttgc tgccgcgcgt gcacggctcc
     9961 gccctcttct cccgcggcga aacgcaagcc atcgccgtcg ccaccctcgg caccggccag
    10021 gatgagcagg aaatggacgg cctcaccggc ggtgacacca agaagcgctt cacgctgcac
    10081 tacaacttcc cgccctactc caccggcgaa gtcaaacgcc tcggcggcct caaccgccgc
    10141 gaaatcggcc acggcgcact cgccgagcgt tccatcgcgc agatgatgcc ggcggactac
    10201 ccctacaccg tgcgggtggt atccgaaatc atgggctcca acggttcttc ctccatggcc
    10261 tccgtttgcg tgggcaccct cgcgctgatg gatgccggcg tgccgctgaa gaagcccgtg
    10321 gcgggcatct ccatcggcct cgtcaccggc gcaagccaaa agcccgtcct gctgaccgac
    10381 atcctcggcg cggaagacca ctgcggcgac atggacttca aggtcgccgg cacccgggac
    10441 ggcatcaccg gcttccaggt ggatctcaaa attcgcggcc tcgagtggga cttggtggct
    10501 ggcgcgttcg accgcgccca tgccgcccgc ctgaagattc tggacaccat gcaagccgcc
    10561 ctgccggcgc cgcgtccgga actctcgccc catgccccgc gcatcatggt cgtccacatc
    10621 aaccccgaaa agatcggcgc gcttatcggc cccggcggca agaatatccg ccgcatcacc
    10681 gaaaccaccg gcacgcagat tgacatcgag gatgacggca aggtctcgat cttctccaac
    10741 aacaaggaca acctcgacgc cgccctgcgg gaagtcgaag ccctcaccgc cgaagcggaa
    10801 gtgggcaaga tttaccgcgg cgccgtcacc ggcatcaagg atttcggcgc gttcgtcgaa
    10861 ttcctgcccg gtcaggaagg cttggttcac atcagcgaac tcgccgactt ccgcgtcaac
    10921 cgcacggaag acgtcgtcaa gatgggcgag gaaatctggg tcaaggtgct cagcgtggaa
    10981 gacaacggca aagtccgcct ctcccgcaaa gccgccatgg ccgagaaggg cgaaaagagc
    11041 cgcaagggtt aaaacaaccc ctgcccccgc cgccgggttc cccccggcgg cttttttgtc
    11101 ggcgcagaac aagggtgcac gacataaaag tcggttatta agccgcgcga tcatcccaga
    11161 agcggtc
//
LOCUS       DAOPNP010000083        11060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6765_length_11060_cov_9.397547, whole genome shotgun sequence.
ACCESSION   DAOPNP010000083 DAOPNP010000000
VERSION     DAOPNP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_11060_cov_9.397547"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKI64_08005"
     CDS             complement(<1..895)
                     /locus_tag="PKI64_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOE00989.1"
                     /translation="MKKLPRPPPPATLPRMKIFVYGSLGLAVLWTAMTSAQADTRMKV
                     AASSTPIYAQANAGAQRVGAATAGEILFVTRTEGDWAAISAPDRLSLWLNKDFIDGNR
                     VVAKSIQVRSGPGIEYSVVGTLERGALVMPRGEDGEWRKIAAPSSATLWVKKSDLTEV
                     RAQSSPIGDVATVPEPKPAPPPPQPAPAPSVPPAPEPPPVPAHTVEVAHAPAPSPSPA
                     TPAPAPAVKVAPSTPGAHPAPAAPSKPAPAPQPTHAAPQPKPATPAHAPAPTAPVLRP
                     ATSLPAPTPAPAPAPSRPAPGS"
     gene            936..2381
                     /locus_tag="PKI64_08010"
     CDS             936..2381
                     /locus_tag="PKI64_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00990.1"
                     /translation="MSQPDTSENSVPAAPEWVRALPAAVAAEVAAARADLPTTAAAAG
                     EALPAAIAAWLLLDPAPAARLLAEWLATQDDRGCLHPAVPVACQLAERIAAVLPNATE
                     WLAAILPALGRSITEAFSCFDPRNTGLPRWDGAASALMPETFTPRGFGVDLAVLLANE
                     TAAFLRLAKDRADLGDAINLAEGEMRELDSWLRDNFWDEEAGAFLALDENNRATPDWS
                     PRGFFPLVWRSATEEMNAGLRARAADVLRQTWTPRAWLLLFVLLLPTTHGSIVAQMRR
                     QGLPDGATVAERAAWTVLSHGADAARARYQQDISRPARWLDAHARQLWRALAVGGLAA
                     LILLLGWGYHQRHRAAATPAELEQQARLACAEDQHEAAAALYARAAQQGDTAYFLYRQ
                     AGEWFHAERFAEAEAVCRDILRREPDSPNVRFNLPLAVLKQGRREEALAMYRALADEL
                     AISASPELAARAQLAAELLARQIELDQPADR"
     gene            2433..2789
                     /locus_tag="PKI64_08015"
     CDS             2433..2789
                     /locus_tag="PKI64_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOE00991.1"
                     /translation="MKKILAGWMGCVALLALATGCFRNTISEIELDIPAMRTQACAAA
                     VEGALAKLGIGSNNVVRTVKLDVDARKGWVTYDNVRLGRRNIEVAVRHAGFAVNDLPA
                     DEAARAQLAPECRGDE"
     gene            2782..4263
                     /locus_tag="PKI64_08020"
     CDS             2782..4263
                     /locus_tag="PKI64_08020"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOE00992.1"
                     /translation="MNKSIDFRWVKNAASGSLHILLVQDGRLLAPDGFSAVAQQELNQ
                     WLKNAKFEGDVAACARPAGLSATYLLLGLGKAKDFHAARWLRGWTTVGRELAKDATVQ
                     RAVLAWPAKAEFPVAAPQLLGLAVEGLLAGSYLFETFKSKKKARRFGVDLVGAEVATA
                     AARAAVRRAEITAATLREVRELANLPANEFGPQEVAAHAKRLAKAAGVSCRVWDQAAL
                     KKEKCGALLAVAQGSRRPGCLIRLAYAGTKRQKPLVVVGKAVTFDTGGISLKPPKNME
                     WMKYDKSGGMAALATVLLAARWKIARPVVAYIPAVENMPGGNAARPGDIVTARNGKTI
                     EVLNTDAEGRLILADALDFAAAEKPAAIVDIATLTGAVIIALGYEATAVMGNDDRLIL
                     DLMLAGETTGERLWQLPLWNEYDQMLQGQFADIKNIGDGTAGTIAGGAFLRQFVPEGI
                     PWAHLDIAGTAWHETATAAHAPGATLVPARLLAEWAANFRLDA"
     gene            4276..5169
                     /gene="xerD"
                     /locus_tag="PKI64_08025"
     CDS             4276..5169
                     /gene="xerD"
                     /locus_tag="PKI64_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOE00993.1"
                     /translation="MRFLLDQFMDYLALERGLSENTRLGYGHDLREFLAYLSRRGYTS
                     LQQVARRDLLDFLLASKQKGLAAPSLARRLIAIKVFFRHLAAEGLLATNVADAMDSPR
                     LWKILPPTLSVAEVDRLLAMPLATTPRGLRDRAILETFYATGLRVSELAGLTLDSLRF
                     DAEYIRCVGKGDKERVVPIGARAIAAVQAWLDQGRPAYAVKSRPVNRAVFLSVRGQPL
                     SRVALWQTIKAYARQAGISKEISPHTLRHSFASHLLANGASLRVIQEMLGHADISTTQ
                     IYTHVDQGRLQAIHQKFHPRA"
     gene            5177..5905
                     /gene="rnc"
                     /locus_tag="PKI64_08030"
     CDS             5177..5905
                     /gene="rnc"
                     /locus_tag="PKI64_08030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOE00994.1"
                     /translation="MTSDCTIHWPDLEARLGHVFARRDLLAAALTHSSYVAENAAPGI
                     PESQRLEFLGDAVLSLVAAEWLVAHCAGWREGTLTKVRSRLTNATTLARVARRLQLEE
                     CLRWGRGLAQQTGRARHKPLADSLEALLGALWLDGGLPVVRAVFRTWFADEIHAALAA
                     GSDDNPKGNLQELLQKNHDPAPHYEVITQDGPPHAPHFVCAVFCGDRQLGEGAGPSKR
                     EAEVNAARAALRRLHEQTGEHLLN"
     gene            6053..6484
                     /locus_tag="PKI64_08035"
     CDS             6053..6484
                     /locus_tag="PKI64_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOE00995.1"
                     /translation="MRWMATLLMVCALATGCESDKDNGGGGKLVGTKWRLQAWSASSL
                     DPTRFNITAEFGDAQISGTAAVNLYGGPYTASDGNFSVGDLYITQMAGSEAAMRAEAI
                     YMDLLGQARHYEVTGTTLTLRNASHQTTLVFRSTGQKIPEF"
     gene            6773..7066
                     /locus_tag="PKI64_08040"
     CDS             6773..7066
                     /locus_tag="PKI64_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE00996.1"
                     /translation="MLLLLAIPAGWRPDGGWSGLHALFVSLAIVVVVHGICLLGLVGV
                     LISRLAKKEPIGRGLGMALLYFALLVGGSVLMNGPQELWSDISVLIRSLFTRL"
     gene            7526..7783
                     /locus_tag="PKI64_08045"
     CDS             7526..7783
                     /locus_tag="PKI64_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOE00997.1"
                     /translation="MKDTKKTTVKKAAPKAPKAPKVAKPAAKPVTKPAAKAASKPAPT
                     PEERYRMIQEAAYYIAERHGFNGDSAYFWSLAEAEINAKLN"
     gene            complement(7983..8462)
                     /gene="gspG"
                     /locus_tag="PKI64_08050"
     CDS             complement(7983..8462)
                     /gene="gspG"
                     /locus_tag="PKI64_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOE00998.1"
                     /translation="MTATTPFPALSLRQFLTARSGFTLVEILVVIAIISLLAGVVLLN
                     VAPQLGMGKQAAARAQIKVFASALDTYNMANGRYPTQAQGLDALVRKPTQPPIPDNYP
                     DGGYLQGRTIPHDPWKNPYVYLIPGRQNEPFEVISYGADGEPGGQGNNADLSSSDAQ"
     gene            complement(8527..10782)
                     /locus_tag="PKI64_08055"
     CDS             complement(8527..10782)
                     /locus_tag="PKI64_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HOE00999.1"
                     /translation="MKPISRAELVYGFTPEPGLVAVEHLPGETTDAMTLFVRRDGRLL
                     TRQEPFTPFLWLTKAEWLQGFDPPPDTVRLAGDNPLRYLAQFPTWNALEKALAHLRKA
                     TGRTPSDPGAPYFVLRDPVQQFLLATGRTSFGEMVLPDLSALYVDIETDCDPARDFSN
                     AERPEDRILAIALADGQGWELVLDGRELDERRLLQEFCRVVRQRDPDLIAGHNLFRFD
                     LPYLVTRAKRNRVALDLGRNGSLLTARASRYTAAERTVAFTHYHVHGRHLADTYLLAL
                     GYDVSYRALEHHSLKAVARHFGVAAPGRTYLEGRQIAATFRTDHAAFLRYARDDIRET
                     AAIAPILLNAFFAQSMLLPLPLEAVCLRGTASKVEALLLREYLRTRQSIPAPDPPQAF
                     AGGYTDLFYEGLARNVHHCDVRSLYPSILLREGRNPRHDAGGVFLQLLAHLRDFRLEA
                     KQRAREPGQPPAVAGRWEALQTTFKLLINSFYGYLGFPLARFNDYDLAASVTEIGREI
                     LRGMVATLRDLGAQPIEIDTDGIYFTPPEGLTASTLADFHERFRAHLPPGIEVEFDGQ
                     YPAMFSYKMKNYALLEADGAVIIKGAALKSRGLEPFLRDHLRDWLTLTLNGRAEDIPA
                     MTARRREDIAQRRLPITALAKTEWLSDSPATYQRKREAGGKSRQAAYEVALASGRGYQ
                     AGDAVSYYVTGEKKSVPVHAHAKLVSAFNPQQRDENVAYYVAKLDALIKKLDARLPES
                     EPPNDDQPTLF"
BASE COUNT         2020 a         3276 c         3706 g         2058 t
ORIGIN      
        1 cgctgcccgg agccgggcgg gagggggcgg gcgccggagc gggagtcggc gcggggaggc
       61 tggtggccgg gcgcaatacc ggtgcggtgg gcgcgggcgc atgagccggc gtggcgggtt
      121 tgggttgagg cgcggcatgg gtgggctgcg gcgcaggggc cggcttgctc ggcgcggcgg
      181 gcgcagggtg cgctcctggt gtggagggcg caaccttcac ggccggcgcg ggcgcaggag
      241 tggcggggga cggggacggc gccggcgcat gcgccacttc caccgtgtgg gccggcaccg
      301 ggggtggctc gggggcgggc ggcacactgg gtgcgggggc cggttgcggt gggggcggcg
      361 cgggcttggg ctcgggcacc gtcgccacgt cgccgatggg cgaactctgg gcgcgcactt
      421 ccgtcagatc gcttttcttc acccacagcg tagcggagga gggcgcagcg attttacgcc
      481 attcgccatc ttcgccgcgc ggcatcacca atgcgccgcg ttccagcgtg cccaccacac
      541 tatattcaat gccggggccg gagcgtacct gaatgctctt ggccaccacc cggttgccgt
      601 caatgaagtc cttgttcagc caaagactca ggcggtcggg cgcagagatg gcggcccagt
      661 cgccttccgt gcgcgtaaca aacaaaattt caccggcggt ggctgcgccg acccgctgcg
      721 cgccggcatt ggcctgcgca tagatggggg tggacgaagc cgccactttc atccgggtat
      781 cggcttgggc gcttgtcatt gccgtccaca agaccgccag ccccaaactg ccataaacaa
      841 aaattttcat gcgcggcagt gtagcggggg gtggggggcg aggcaacttt ttcatttgtt
      901 gcccggcggg ataatgcgca atatgtccgg cagccatgag ccagcccgac accagcgaaa
      961 attccgtccc cgccgcgccg gaatgggtgc gcgcgttgcc ggcggcggtt gccgccgagg
     1021 tggccgcggc tcgcgcggat ttgcccacga ccgccgcggc ggcgggcgaa gcgctgcccg
     1081 ccgcgattgc cgcatggctg ctgctggacc ccgcccccgc cgcgcggctc ttggccgaat
     1141 ggctggccac ccaggatgac cgcggatgcc tgcaccccgc cgtgcccgtg gcctgccaat
     1201 tggccgagcg tattgccgcc gtgctgccca atgcaaccga atggctcgcg gccatattgc
     1261 cggcgctggg ccgctccatt acggaagctt tttcctgctt tgatccgcgc aataccggcc
     1321 tgccgcgctg ggatggcgcg gcaagcgcgc tgatgccgga gaccttcacg ccccgtggct
     1381 ttggcgtgga tttggcggtg ttgctggcca acgaaaccgc cgcatttctg cgcttggcca
     1441 aagaccgcgc cgatcttggg gacgcgatca atctggctga aggggaaatg cgcgagctgg
     1501 atagctggct cagggataat ttctgggatg aagaagccgg cgcgttcctc gcgctggacg
     1561 aaaacaaccg cgcgacaccc gattggagcc cgcgcggatt tttcccgctg gtgtggcgct
     1621 cggccacgga ggaaatgaat gcggggctgc gcgcccgcgc cgcggatgtg ctgcggcaaa
     1681 cctggacccc gcgcgcatgg ctgctgctgt ttgtgctgct gttgccgacg acacacggca
     1741 gcatcgtggc gcagatgcgt cggcagggat tgccggacgg cgcgacggtg gcggaacgcg
     1801 ctgcgtggac ggtgctgtcg cacggcgcgg atgccgcgcg tgcgcgttat cagcaggata
     1861 tttcgcgtcc cgcgcgttgg ctggatgccc atgcgcgcca gttgtggcgc gcgctggccg
     1921 tcggtgggct ggccgcgttg attttgctgc tgggctgggg atatcaccag cgccaccgcg
     1981 ccgcggccac ccccgccgaa ctggagcaac aggcccgcct cgcttgcgcg gaagaccagc
     2041 acgaagccgc ggccgcgctc tacgcgcgcg ccgcgcagca aggtgacacc gcctatttct
     2101 tgtaccggca agccggcgaa tggttccatg cggagcgttt tgcggaagcc gaagctgtgt
     2161 gccgcgacat cctgcgccgg gagccggatt cgcccaacgt gcgcttcaat ctgccgttgg
     2221 cggtgctgaa gcagggtcgc cgcgaggagg cgttggccat gtatcgcgcg ctcgccgatg
     2281 aactggccat ttccgcctcg cccgagctgg ccgcgcgcgc gcaactggcg gccgaactgt
     2341 tggcacgcca gatcgagctg gatcagcccg ccgatcgttg acaggggacc cgtgcggtgg
     2401 aatagtgacc ggactgcttt gggagataca ccatgaagaa aatattggcc ggctggatgg
     2461 gttgcgttgc gctgttggcg ctggcgacgg gctgcttccg caatacgatt tccgagattg
     2521 aattggatat tcccgccatg cgcacacagg cctgcgcggc ggcggtggaa ggcgcgctgg
     2581 ccaagctggg catcgggtcg aataacgtcg tccgcacggt gaagctggat gtggacgcgc
     2641 gcaagggctg ggtcacctat gacaacgtgc gtttgggccg gcgcaatatc gaggtggccg
     2701 tgcgccacgc gggattcgcc gtgaacgacc tgcccgccga tgaagcggcg cgcgcccaac
     2761 tggccccgga gtgccgcggc gatgaataaa tccattgatt tccgttgggt gaaaaacgcg
     2821 gcgtcggggt cgctgcacat tttgctggtg caagacggcc gcctgctggc gccggatggc
     2881 ttctccgccg tcgcgcaaca ggagctgaac cagtggctca agaatgccaa attcgagggc
     2941 gatgtggcgg cttgcgcgcg gccggcgggg ctctcggcaa cctatttgct gctgggcttg
     3001 gggaaggcca aggatttcca cgcggcgcgc tggctgcgcg ggtggaccac cgtcgggcgt
     3061 gagttggcca aagatgccac cgtccagcgc gctgtgttgg cgtggccggc caaggcggag
     3121 ttcccggtgg ccgcgccgca gttgctgggc ttggcggtcg agggcctgct ggcgggcagc
     3181 tatttgttcg agacgttcaa atccaagaaa aaggcacggc gctttggcgt ggacttggtc
     3241 ggcgcggaag tcgccaccgc ggcggcgcgc gcggcggtgc gtcgtgctga aatcaccgcg
     3301 gccactttgc gcgaagtgcg cgagctggcc aacctgccgg ccaatgaatt cgggccgcag
     3361 gaagttgccg cgcacgcaaa gcggctggcc aaggctgccg gtgtttcgtg ccgcgtgtgg
     3421 gatcaggccg cactgaaaaa agaaaaatgc ggtgcgttgc tagcggtggc gcaaggtagc
     3481 cgccggccgg gttgcctcat tcggctggcc tatgccggca ccaagcggca aaagccgctg
     3541 gtggtcgtcg gcaaggccgt gaccttcgat accggcggca tcagcctcaa gccacccaaa
     3601 aacatggagt ggatgaagta tgacaagagc ggcggcatgg cggcgctggc caccgtgctg
     3661 cttgccgcgc gttggaaaat cgcgcggccc gtggtggcct atatccccgc cgtggagaac
     3721 atgcccggcg gcaatgccgc gcgccccggc gacattgtca ccgcgcgcaa cggcaaaacc
     3781 atcgaagtgc tcaacaccga tgccgaaggt cgcctcatat tggccgacgc gctggatttt
     3841 gccgccgcgg aaaaaccggc ggctattgtg gacatcgcca cgctgaccgg tgcggtgatc
     3901 attgctcttg gctacgaggc caccgccgtc atgggcaacg acgaccgcct tattctggac
     3961 ttgatgctcg ccggcgaaac cacgggcgaa cgcctctggc aactgccgct atggaatgaa
     4021 tatgaccaga tgctgcaagg ccagtttgcc gacattaaaa acatcggtga tggcaccgcc
     4081 ggcaccatcg ccggtggcgc gttcttgcgc cagttcgttc ccgaaggcat tccgtgggcg
     4141 catctggata ttgccggcac ggcctggcac gaaaccgcca ccgcggccca cgcccccggc
     4201 gccacgctgg tgccggcccg cctgctggcg gagtgggccg cgaatttccg gctggatgcc
     4261 tgacccgccg ccggcatgcg cttcctgctc gatcagttca tggattacct ggcgctcgag
     4321 cgcgggctaa gcgaaaatac acggctgggc tacggccatg acttgcggga gttcctcgcg
     4381 tatctctcgc gcaggggtta caccagcctg caacaagtgg cgcggcgcga cctgctggat
     4441 tttctgttgg cgagcaaaca aaagggattg gccgcgccga gcctcgcccg ccggttgatt
     4501 gccatcaagg tattctttcg tcatctggcc gccgaaggcc tgttggccac caacgtcgcg
     4561 gatgccatgg attcgccgcg cttgtggaaa atcctgccgc cgaccttgag cgtggccgag
     4621 gtggaccggc tgttggccat gcctctggcc accacgcccc gcggcctgcg tgaccgggcc
     4681 attttggaaa ctttttatgc caccggcctg cgtgtcagcg agctggccgg gctgacgctc
     4741 gattcgctac gcttcgatgc cgaatatatc cgctgcgtgg gcaagggaga caaagagcgc
     4801 gtggtgccca tcggcgcgcg cgccatcgcc gcagtgcagg catggctgga ccagggccgc
     4861 cccgcctatg ccgtcaaaag ccgccccgtc aaccgggccg tttttttatc agtgcgcggc
     4921 cagccgctga gccgcgtggc gctctggcaa accatcaagg cctacgcgcg ccaagccggc
     4981 atcagcaagg aaatttcgcc gcacaccttg cggcattcgt tcgcctccca cctgctggcg
     5041 aatggcgcct cgttgcgcgt cattcaggaa atgctcggcc atgccgacat ctccaccacg
     5101 cagatttaca cccacgtgga ccaaggccga ttgcaggcca tccaccagaa attccacccc
     5161 cgcgcgtgaa tccgccatga cgtctgattg caccatccat tggcccgatt tggaagcccg
     5221 cttgggccac gtctttgctc ggcgcgactt gctggctgcc gcgcttacgc acagctccta
     5281 cgtggccgaa aacgcggccc ccggcattcc cgaaagccag cggctggaat ttttgggcga
     5341 tgccgtcttg agcctcgtgg cggcggagtg gctggtggcc cattgcgcag gctggcgcga
     5401 aggcacgctc accaaagtac gcagccggtt gaccaatgcc accacgctgg cgcgagtggc
     5461 ccgccgcttg caactggaag aatgcctgcg ctgggggcgc ggccttgcgc agcaaaccgg
     5521 gcgggcccgc cataaaccac tggccgactc cctggaggcg ttgctcggtg cgctgtggtt
     5581 ggatggcggc ctgcccgtcg tgcgcgcggt gttcaggacg tggtttgccg acgaaatcca
     5641 cgccgcactc gccgccggca gcgacgacaa ccccaaaggc aatttgcaag agctactgca
     5701 aaaaaaccac gatcctgcac cgcactacga ggttatcact caagatggcc cgccccacgc
     5761 cccgcatttt gtctgcgcgg tgttttgcgg cgaccgccag ttgggcgagg gggccggccc
     5821 cagcaagcgc gaggccgaag tcaacgccgc ccgcgccgcc ctccgccggc tgcacgaaca
     5881 aacgggcgag catttgctca attgagcggg ccttttgcaa ttcagcaacc ggccccaggg
     5941 gggggggatt ggacgcgcgt tttttcttta tgcggatgaa gcccagggta gaatgccggc
     6001 ttgtcgtgtt ctctggggtg atgtgttttg ggatttagct tgggagacga gcatgagatg
     6061 gatggcgact ctgctgatgg tgtgcgcgct ggcgacaggt tgtgagagcg acaaagacaa
     6121 tggcggcggc ggcaagctgg tcgggacgaa gtggcggctg caagcgtggt cggccagttc
     6181 gttggaccca acgcggttca atatcaccgc cgagtttggc gatgcccaaa tatccgggac
     6241 agcggcagtc aatttgtacg gcgggcctta cacggccagc gacggcaatt tttccgttgg
     6301 cgacttatac atcacccaga tggccggttc cgaggccgcc atgcgcgccg aggcgatcta
     6361 tatggacttg ttggggcagg cccggcacta cgaggtgacg gggaccaccc tcacgctccg
     6421 caacgcgagc catcaaacca cgctggtttt ccgatccacc ggccaaaaaa tcccggaatt
     6481 ttaggggacc gtcaaatggc aggcgctgga aagcagattt tttgccattg atgctgacaa
     6541 cgggcagaag tatgaaccca ttagcctgcc cgaggcttat cgagtcagcg ggttgcgtgt
     6601 tcatgtgcag gccaaagaac gcggcgattt ggcgagcatc aacatgcacg gcacgatgat
     6661 tgaaatcatc agcatcgcca aattgtgatg cgtctacgcc gccgggtgac agggtaggtt
     6721 gatcatgcgc aagattgtgc ccctgtgtgg ggttggggca cccgcttcgt ggttgctgct
     6781 gctgctggcg attccggccg gatggaggcc ggatggcggc tggagcggcc tgcacgcttt
     6841 gtttgtgtcg ttggcaatcg ttgtggttgt gcatgggatt tgtttgcttg ggctggtggg
     6901 tgtgttgatc agtcgtctgg ccaaaaaaga accgattggg cgcgggctgg ggatggcatt
     6961 gctttatttt gcgctgttgg ttggcggcag tgtgctgatg aacggaccgc aagagctttg
     7021 gagcgatatt tcggtgttga tcaggtcgct tttcacgcgg ctatgacgcg cccgccggcg
     7081 actggcatcg cccactagca gggggggcgt ttgaaaaaaa gaatctcgcc aaggcgccaa
     7141 gaacgcaacg aagagcgagg caacttcctt caaaaaacgt cgttgggcgg tccgccgtac
     7201 gggcctacac tacggcgaca aaaagggggt ggcagggaaa aatgtcaaaa catgattttg
     7261 gcgggataga tcattaaaat cgcgttagaa ctgacttgct tggttaatct gcgagggtaa
     7321 aacgatttat ttttataaaa atctatctta ttgcccgcct agggaatacg ttaaaaccac
     7381 gggaacttgt tcgttttttc cgcaaaaaag gtcttgcaaa cccaacgtgg tgcaataaag
     7441 ttcgcgcaga tttgcacccc gctgtgggga gcaggtgtca acccaatcaa cgcttcggac
     7501 cgccccgagg caggagttct gcaaaatgaa ggatactaaa aagacgacag tgaaaaaggc
     7561 cgccccaaag gcgccgaagg cccccaaggt tgccaagccg gctgccaagc cggtgactaa
     7621 acccgccgcg aaggctgcca gcaagccggc gccgactccg gaagagcgct accgcatgat
     7681 ccaagaagcg gcgtactaca tcgccgagcg tcatggcttc aatggcgaca gcgcctattt
     7741 ctggtcgctt gccgaagcgg aaatcaacgc gaagttgaac taaacttatt gaccgtgatg
     7801 ggtcaagacg gccgcaggtt cgcctgcggc ttttttgttg gatgtccgaa ttcggattgt
     7861 gggatggaaa tagtatcagg ccttccaatc tttttcggtg ggaaggtttc gttcctcaac
     7921 cattcccgcc cgacaaaagc atccggcgac gacctcccag ctacattatt ttttcaaatg
     7981 ctctattgcg cgtcgctgga ggataaatcg gcgttgttgc cttggccgcc gggctcgcca
     8041 tcggcgccgt aggagatgac ctcgaacggt tcattttgcc ggccggggat caggtacacg
     8101 tacggattct tccagggatc gtgggggatg gtgcggcctt gcaggtagcc gccatcggga
     8161 tagttatctg gaatgggggg ctgggtgggc ttccgcacca gcgcatcgag gccctgggcc
     8221 tgcgtgggat aacggccatt ggccatgttg taggtgtcga gcgccgaagc gaacaccttg
     8281 atttgcgcgc gggcggcggc ttgtttgccc atgccgagtt gcggggccac attcagcagc
     8341 accacgccgg ccagcagcga aataatggca atcaccacga ggatttccac cagcgtgaac
     8401 ccgctgcgcg cggtcaagaa ttggcgcagg gagagagcag ggaacggggt tgttgcagtc
     8461 atgatgggtt ctccttgcgc gccattgggg gacaccgcca gccggtgggc agttccgcgg
     8521 ctgcgttcaa aataatgtgg gctggtcatc attgggcggc tcactttccg gcaggcgagc
     8581 atccagtttt ttaatgagcg cgtcaagttt ggctacgtag taggccacgt tttcatcgcg
     8641 ctgctgcgga ttgaacgcag aaaccaactt ggcgtgggcg tgaacgggca cgctcttttt
     8701 ttcgccggtg acgtaatagg acacggcatc acctgcttga tagccgcggc cggatgccaa
     8761 cgccacttcg taggctgcct gccgcgactt gccgccggcc tcgcgcttgc gctggtaggt
     8821 ggcgggagag tcggaaagcc attccgtttt ggccagcgca gtgatgggca gccgtcgctg
     8881 ggcgatgtcc tcccgccgcc gggcggtcat ggcgggaatg tcttccgcgc ggccgttgag
     8941 cgtcagcgtc aaccagtcac gcaagtggtc gcgtaaaaac ggctccagcc cgcgcgattt
     9001 gagcgcggcg cccttgatga tcaccgcgcc gtcagcctcc aacagcgcgt agtttttcat
     9061 tttataggaa aacattgccg gatattggcc gtcgaattcc acctcgatgc ccggtggcag
     9121 atgggcgcgg aagcgttcat gaaaatcggc cagcgtgctg gcggtgaggc cttccggtgg
     9181 ggtgaagtag atgccgtcgg tatcaatctc gatcggttgc gcgccgaggt cgcgcaaggt
     9241 ggcgaccatg ccacgcagaa tctcgcgtcc gatttccgtg accgaagcgg ccagatcata
     9301 atcgttaaag cgggcgagcg ggaagccgag atagccgtaa aacgagttga tcagcaactt
     9361 gaacgtggtt tgcagcgctt cccagcggcc ggccaccgcg ggcggttggc ccggctcgcg
     9421 cgcgcgctgc ttggcttcga ggcggaagtc gcgcaggtgt gccagcagtt gcaaaaacac
     9481 accgccggca tcgtgccgcg ggttgcgtcc ctcgcgcagg aggatggaag gatacagcga
     9541 gcgcacgtcg cagtggtgta cgtttcgggc gagaccttcg tagaacaggt cggtatagcc
     9601 gccggcaaaa gcctgtggcg gatcgggcgc agggatggac tgccgggtgc gcaggtattc
     9661 gcgcaggagc aacgcctcga ctttgctggc ggtgccgcgc aggcacacgg cctccagcgg
     9721 cagcggcaac aacatgcttt gcgcgaaaaa ggcgttcagc agaatgggtg caatggccgc
     9781 ggtttcgcga atgtcgtcgc gggcatagcg caggaaggca gcgtggtcgg tgcggaaggt
     9841 ggcggcaatc tgccggcctt ccaaatacgt gcggccgggc gccgccacgc cgaagtgccg
     9901 tgctactgcc ttgagcgaat gatgctccag cgcgcggtac gaaacatcat agcccaacgc
     9961 cagcaaatag gtgtccgcca agtgccggcc atgcacatga tagtgcgtga aggccaccgt
    10021 gcgctccgcg gcggtatagc gcgaggcgcg cgcggtgagg agggagccat tgcgcccgag
    10081 gtccagcgcc acacggttgc gcttggcgcg ggtgaccaaa tagggcagat caaatcggaa
    10141 caagttgtgc cccgcgatca agtcgggatc gcgctggcgc accacgcggc aaaattcttg
    10201 cagcaggcgg cgctcatcga gttcgcggcc atccagcacc agctcccaac cttggccatc
    10261 ggccagcgcg atggccaaaa tacgatcttc cgggcgttcg gcattggaaa aatcgcgcgc
    10321 ggggtcgcaa tcggtctcga tgtctacata tagcgcggac aaatccggca gcaccatctc
    10381 gccgaacgaa gtccgcccgg tcgccagcaa aaattgttgc acgggatcac ggaggacaaa
    10441 gtagggcgcg ccggggtccg atggtgtgcg ccccgtggcc ttgcgcagat gggccagcgc
    10501 tttttccagc gcgttccacg tgggaaactg cgccagatag cgcaagggat tatcgcccgc
    10561 caaccgcacg gtgtcgggag gcgggtcaaa gccttgcagc cattcagcct tggtcagcca
    10621 caaaaaggga gtgaacggtt cttggcgggt cagcaggcgg ccatcgcgcc gcacaaacag
    10681 cgtcatggca tcggtggttt caccggggag atgctccacg gccacgaggc cgggctccgg
    10741 ggtgaaccca tagaccaatt ctgcgcggga gatgggtttc atgtcagcca ccagcataaa
    10801 cccgctgctg caccctgtcg aggcttcaag gcgagccaaa tcattgcgta agatttttgc
    10861 gatttgacat tcgagcggca agtaatgtgc agaagcgctc ttaccaacgt ttttgccata
    10921 tatttaatgc caactgcctc tggctcaagg aggtcgattc ccctttgtca caaatcgtcg
    10981 acacttctcg ctacatttgc agcgaggtca attgacaaag taacggcgca gtttgggggt
    11041 tatgggcggc aagagttgta
//
LOCUS       DAOPNP010000084        10953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6843_length_10953_cov_9.734814, whole genome shotgun sequence.
ACCESSION   DAOPNP010000084 DAOPNP010000000
VERSION     DAOPNP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6843_length_10953_cov_9.734814"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKI64_08060"
     CDS             complement(<1..501)
                     /locus_tag="PKI64_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE01000.1"
                     /translation="MDLTQLVLSRHSTKAFDPARKISGAVMESVRALLRNCPSSVNSQ
                     PWHFVIAASEVGKARIARATGGPYAYNGPKIQNASHVVVLCAKRDLDDAAVAERVAQE
                     MRDGRFAEAADAEKQRASKAFYVSLHRDTRHDLPHWLEKQTYIALGSLLLGAAALGLA
                     ACPMEGF"
     gene            complement(571..1704)
                     /gene="mutY"
                     /locus_tag="PKI64_08065"
     CDS             complement(571..1704)
                     /gene="mutY"
                     /locus_tag="PKI64_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOE01001.1"
                     /translation="MPSFSSRLAPIRAAARRRLLPWFARHQRNLPWRAEPRTPYAVWI
                     SEIMLQQTRVDTVIPYYERFLARFPDVAALAAAPRQAVLKAWENLGYYSRARNLHRTA
                     RLLMRDFAGKLPATQAELATLPGLGPYTAAAIASLAFGQPCAALDGNIIRVLARLLAL
                     PDDVARPATRHKLQRVADQLLVRGQPGRTNEAWMELGALVCTPRAPRCAECPLRSVCR
                     AARQPDPTIYPRRKKKMKVPHKVVGAAIILNRRNQILIAQRPPEKGMLAGLWEFPGGK
                     QNPGETMPECIARELHEEMGLRLAVGPRLLVVHHAYSHFTIELHAHFARIVSGRPRHL
                     ECASHAWTTRDGFDDFPFSKADLEIIRALRALPHPPQWRDYFA"
     gene            complement(1795..2820)
                     /locus_tag="PKI64_08070"
     CDS             complement(1795..2820)
                     /locus_tag="PKI64_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOE01002.1"
                     /translation="MKILNIVAWVLVVVLGVAAGTAGFLIKKQSDRANGLQNALAQVA
                     TAVGVEETDTAAPLANKLEIVQAAIEHAQQELAATKAGLSAAQTEATEAKAQVATLTQ
                     AAQEQSAKLDSLAQEVAAKDEAMAAAQAAAAKAQEDADAALAQAGKEKAALEKDLARA
                     QKKLAEEKARLEAELAAVQSNEESDLETVDFPLVGAEELPPVVAAPVAQPADEACPED
                     VSPAEFAASRILGQSPMLSLLRYNPEDQTLYVKLLDGQTLTYQDVPEGVYAAFVADPD
                     KLDMNFRFKIQGIYKSLPPDSVVIRKYWKWQRKHRYPQGDVRVIDPPEKKVAPAPAEE
                     ADATVAD"
     gene            complement(2951..3700)
                     /locus_tag="PKI64_08075"
     CDS             complement(2951..3700)
                     /locus_tag="PKI64_08075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE01003.1"
                     /translation="MTEKQQQNTSDGAAGVRLQKVLAAAGIGSRRHCEELIVAGRVQV
                     DGQVVTQLGTRVDPARQQVALDGRVLRAEPLRTFLVDKPRGILCTAHDPEGRPTIGEW
                     AREMGAPLQRLYTIGRLDYDSEGLILLTNDGHLAQRMAHPRYQTEKEYRAWTDRAPTR
                     EEVAAMLAGIEDNDERLRALAVRVKRTGAHPLIRIVLGEGRNRHIRRMLGALGIKTHR
                     LRRVRLGPLTEKDLRGRRLRELSAAELARLR"
     gene            4208..6343
                     /gene="ligA"
                     /locus_tag="PKI64_08080"
     CDS             4208..6343
                     /gene="ligA"
                     /locus_tag="PKI64_08080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOE01004.1"
                     /translation="MERPVQASAKVAERAAFLRAELTRHNHLYYVEAQPEISDREFDR
                     LLRELQDLEAAHPELQTADSPTQRVGGAPLTGFQQVKHAVPMMSLDNTYSAEELREFD
                     GRVCKALGVERVEYVVEPKVDGVSISLRYEHGVLVQAATRGDGRTGDDITANARTIRS
                     IPLRIESATPVLEVRGEVYLGHAAFARLNEERAAAGEPLFANPRNATAGSLKQLDPKV
                     VAARPLAAVFYATGEWTGPEFATQAELLRGLKQLGFPVAALWWECRDIAAVIERAMEL
                     QQREGELAYDMDGAVVKVNRLALWRQLGATAKAPRFAMAYKYSREQAQTILRGITLQV
                     GRTGVLTPVAELEPVELAGSTIARATLHNEDEVRRKDIRVGDTVVIEKAGEVIPAVVA
                     VVPDLRPPGTQPFDLFAHAGGRCPSCGQPISRDPAAAAWRCTNDDCPAQVRGRLEHFV
                     ARKAMNIDGLGEAILAALASPVTVQEKVDDGLFGQKIEERVLPPVVHDVADLYALTPE
                     DIELRRPNRALDSKAASMKLATKLCAAVAASKNNELWRLIHGLGIPNVGEGLARSLAQ
                     ELGSLDALLAASAETLAGVRDVGDIVAQSVVDYFANPRNRETVEKLRRAGVRFDRVER
                     AAAGASTDGFFFGKRVVITGTLAKFTRQQAQEELRRRGATVAETVGKTTQILVAGEAA
                     GSKLAKAQKLGITILDEVAFLEKLGETNG"
     gene            complement(6347..6913)
                     /locus_tag="PKI64_08085"
     CDS             complement(6347..6913)
                     /locus_tag="PKI64_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOE01005.1"
                     /translation="MAPATNSSPAPRPCVFFDRDGIVNRSPGPGYVNHLDDFHILPGF
                     ADCVRAAAAKGWPAVIVTNQRGISRGITPPDQLAAMHQRLHDELARAGLALLDILICT
                     ADDAAHPNRKPNPGMLFDAARRHHLDLARSWIIGDRESDVQTGHNAGLAVTVLVDPAA
                     PATAATYCVPDMPACAALLADKLPPASA"
     gene            complement(6897..8303)
                     /locus_tag="PKI64_08090"
     CDS             complement(6897..8303)
                     /locus_tag="PKI64_08090"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOE01006.1"
                     /translation="MLRRSFKFQSELAAIVDALLVAGALLAALVTHKMLVWLFPGYFA
                     IFDMFWSNSWLYVLVVPVWIFVLDFSGFYRQFIGLPHARIFQQAARGGILSFAVLLGL
                     LYAFKLHAVPRTILGIHAIYSSLAVALRAIYLQPYILRLEARRRLLFIGSPARAAGIR
                     AWLSHPTRARFFEIVGFLSPAGTTPPPDFPAAGTLDDFSAILHSTVVDAVVILPDDVS
                     TAQVTQWLRQCEIEGIEAWLLPPYLHTTIAETTLDEVAGDPVLLFTTTAKSSWALLFK
                     RLLDICGAAIGLIVLSPLLLGIALAIRLTSPGPIFFTQRRSTLRGREFNMIKFRTMVA
                     NAEQIRAELEQLNEVTGPVFKIKDDPRITPVGRWLRRYSLDELPQLWNVLVGDMSLVG
                     PRPPIPAEVAKYENWQRRRLSMRAGCTCLWQVGGRNDLPFEEWMRLDLKYIDTWSLGL
                     DFQILLKTVGVIFRGTGY"
     gene            complement(8349..8879)
                     /locus_tag="PKI64_08095"
     CDS             complement(8349..8879)
                     /locus_tag="PKI64_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE01007.1"
                     /translation="MSRAAPQSPRILAATADNCTLIVVSGAATFKLAPAFKQALQAAK
                     LAGSPLVVVDMADCVSMDSTFMGALATAGLAQRTSDCRLALLNPTPHARSLLRGLGVD
                     RLLKSYSAAELPAGLADLSGLKQNLRPVEAAPVSQRDMAALMYDAHETLTRVDPENLQ
                     RFKDVLAFLREDLQRF"
     gene            complement(8882..9343)
                     /locus_tag="PKI64_08100"
     CDS             complement(8882..9343)
                     /locus_tag="PKI64_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01008.1"
                     /translation="MVRRGAGVALAMLLMPAVVRAEPYTYVARAHIIGVQQSFLYQAT
                     PSVPGAGVRAFQDPDSETGGRERFDIRWYANPPGIPPGTVLLLETVQERSPVVRSYFL
                     RTTDKSEGNTLSVLEIPPDKVRQGGRVIKWRLRVVWRGKVLATQTSANWTS"
     gene            complement(9408..10406)
                     /locus_tag="PKI64_08105"
     CDS             complement(9408..10406)
                     /locus_tag="PKI64_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOE01009.1"
                     /translation="MKYLDYYAVLGVPRTATAEEIKKAYRKLARQYHPDVNKTAGAET
                     RFKEINEANEVLSDPEKRKRYDALGANWKAGQNFTPPPGWGGFGGASSGPGGIRFEFG
                     DSGGGDFSDFFSALFSDLAGAGRSGRSRRSSPFTDFGGFGAGSPEEAFQPRGQDFEAT
                     LAITLEDLINREPKAITLQVPVVRPNGAVAHEAKTFHVRIPTGATEGTRIRLSGQGAA
                     GGNLYLNLRIAPHPIFQLNGYDLDQVVAIAPWEAVLGAKVGIPTLEGPATLSIKPGTQ
                     NGARLRLKGKGLPNKTGSRGDMLVTVRIVVPDRPTAKEKALFEQLARESSFQPRPR"
     gene            complement(10419..10694)
                     /locus_tag="PKI64_08110"
     CDS             complement(10419..10694)
                     /locus_tag="PKI64_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01010.1"
                     /translation="MKTMMLAATLSLLLAGGVFAESPPDDAVTRDVRNLQHDIEDLTA
                     RLNKIEDRLDTVEARLGRTFRAPSPFDNVERRLDDLEKDVQDLKRRR"
     gene            10867..10953
                     /locus_tag="PKI64_08115"
     tRNA            10867..10953
                     /locus_tag="PKI64_08115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10901..10903,aa:Leu,seq:cag)
BASE COUNT         2064 a         3121 c         3774 g         1994 t
ORIGIN      
        1 aaagccttcc attgggcagg cggccaagcc gagcgccgcc gcgcccagca atagcgaacc
       61 gagggcgata tacgtttgtt tttccagcca atgtggcaaa tcgtggcgcg tgtcgcggtg
      121 caaggacacg taaaaggcct tggaggcgcg ctgtttttcc gcgtcagcgg cttcggcaaa
      181 acggccatcg cgcatttcct gggccacgcg ctcggccacc gcggcatcgt cgagatcgcg
      241 cttggcacac agcaccacca catgcgaggc gttttggatt ttggggccgt tgtaggcgta
      301 ggggccgcct gtagcgcggg cgatgcgcgc tttgccgact tcgcttgcgg cgatcacaaa
      361 atgccagggc tgcgagttga cggacgacgg acaattgcgc agcagcgcac gcacgctctc
      421 catgaccgcg ccggaaattt tgcgcgcggg gtcaaaggcc ttggtggagt ggcgggacaa
      481 aacaagttgg gtcaggtcca tgagaaagct cctcggttgc atgtggcggc aatgtaatgc
      541 accgcgtgcg cggcggcaag cagcggcgtg tcaggcaaaa taatcacgcc actgcggcgg
      601 gtgcggcagg gcgcggagcg cgcggatgat ttccagatcg gctttcgaga atggaaaatc
      661 gtcaaacccg tcgcgggtgg tccacgcatg gctggcgcac tccaggtggc gcgggcgacc
      721 ggacacaatg cgcgcgaagt gcgcgtgcag ttcgatggtg aaatggctgt aggcgtggtg
      781 gaccaccagc aggcgcgggc ccacggccaa ccgcaggccc atttcctcgt gtagctcgcg
      841 ggcaatgcac tcgggcatgg tttcgccggg attttgtttg ccgccgggaa actcccacaa
      901 tcccgccaac attccttttt cgggcgggcg ctgcgcaatc aggatttgat tgcggcggtt
      961 taaaatgatg gcggcgccaa cgactttgtg aggaactttc attttctttt ttcgccgggg
     1021 atagatggtg ggatcgggtt ggcgggccgc gcggcaaacg gaacgtagcg ggcactctgc
     1081 gcaacgcggc gcgcgcgggg tgcacaccag agcgcccagt tccatccatg cctcattggt
     1141 gcggccgggt tgcccacgca caagcagttg gtcggccacg cgttgcagtt tgtgccgtgt
     1201 ggcgggccgt gcgacatcat ccggcagcgc cagcaggcgc gcgagaacgc ggatgatatt
     1261 gccatccagc gcggcgcaag gctggccgaa cgccagcgag gcaatcgcgg cggcggtgta
     1321 cggacccaaa ccgggcaatg tggcgagctc cgcctgggtc gcgggcaact tgccggcgaa
     1381 gtcgcgcatg agcagccgcg ctgtgcggtg gagattgcgc gcccgcgagt aatagcccag
     1441 attttcccac gctttgagca ccgcttgccg cggcgcggcg gccagcgcgg ccacatcggg
     1501 gaagcgcgca agaaaccgtt cgtaatacgg gatgacggtg tccacgcggg tttgctggag
     1561 catgatttca gaaatccaaa ccgcataagg ggtgcggggt tccgcgcgcc agggcaagtt
     1621 gcgttgatgc cgagcaaacc acggcaagag gcgacgccgc gccgccgcgc gaatcggcgc
     1681 taaacggctg gagaaagacg gcatgtattg cgacgagggg gccacgcacc tagttcggcc
     1741 acgcgcccgg gagtgtcgcg tgcgccgggg agggggtggt gggcgtgacc gctattagtc
     1801 ggccacggtt gcgtcagctt cttccgcggg agccggcgcg acttttttct ccggcgggtc
     1861 aatgacgcgc acatcgccct gcggatagcg atgcttccgc tgccacttcc agtacttgcg
     1921 gattaccacg ctgtccggcg gcaagctctt gtagatgccc tggatcttga atcggaagtt
     1981 catatccaat ttgtcggggt cggcgacgaa tgccgcatac acgccttcgg gcacatcttg
     2041 ataggtcagc gtttggccat ccagcagttt cacgtacaga gtttggtctt caggattgta
     2101 gcgcaatagc gagagcatgg gggactggcc caagatgcga ctggcggcaa actcggcggg
     2161 ggagacatcc tctgggcatg cttcgtcagc gggttgtgca actggggcgg cgaccaccgg
     2221 cggtaattct tccgcgccga caagtgggaa atccacggtt tcaaggtcgc tttcttcgtt
     2281 cgattgaacg gcggccaact cggcttccaa acgggctttt tcttcggcca gcttcttttg
     2341 cgcgcgggca aggtcttttt ccagcgcagc tttttccttg ccggcttggg caagggcggc
     2401 atcggcatcc tcctgcgctt tcgcggcagc agcttgcgcg gcggccatgg cttcatcctt
     2461 ggccgccact tcctgcgcga gactgtccag cttggcggat tgttcctgcg cggcctgcgt
     2521 cagggttgcc acttgggcct tggcctccgt ggcctcggtt tgcgcggcgc tcagcccggc
     2581 cttggttgcg gccaactcct gttgtgcgtg ctcaatggcc gcttgcacga tttccaactt
     2641 gttggccagc ggcgccgcgg tatcggtttc ttcgacgccg actgccgtcg ccacttgggc
     2701 gagcgcgttc tgtagaccat tggcgcggtc cgattgtttt ttgatcagga aacccgcagt
     2761 tcccgcggcc acccccaaga cgaccaccaa cacccaagcc acaatattca atatcttcat
     2821 cgcgctctcc tgtgtgtgcg ttacaacagt ggtgaattgg acttgaagcc gtgcggattg
     2881 tcaagacatc gcgcggcgcg gtgtcggcca tttcgctgtc ttgccggatg cgggagggga
     2941 ggggggggcg tcagcgcagg cgggccagtt cggcggccga taactcacgt aggcggcggc
     3001 cgcgcaaatc cttttcggtc agcggcccca agcgcacccg tcgcaagcga tgagtcttga
     3061 tgcccagcgc gccgagcatc cggcggatat gccggttgcg gccttcaccc agcacgatcc
     3121 ggatcaacgg gtgggcaccg gttcgtttca ccctcacggc caatgcccgc agccgttcgt
     3181 cattatcttc tatgccggcc agcatggctg cgacttcctc gcgagtgggg gcgcgatcgg
     3241 tccaggcgcg atactccttt tcggtttggt atcgcgggtg cgccatgcgc tgcgcaagat
     3301 ggccatcgtt ggtcagcagg atcagccctt cgctatcgta atccaagcgg ccgatggtat
     3361 agagccgctg gagcggcgca cccatttccc gggcccattc gccgatggtc gggcggccct
     3421 ctggatcatg cgccgtacac aaaatgccgc gcggtttatc caccaaaaac gtgcgcaggg
     3481 gttccgcgcg cagcacccgg ccatccagcg ccacctgttg cctggcggga tcaactcgcg
     3541 tgcccagttg cgtcaccacc tggccatcca cctgcacccg gcccgcgaca atcaactcct
     3601 cgcagtgccg tcgcgaacca attcccgccg ccgccagcac tttttgcagc cggacccccg
     3661 cggccccatc actcgtattt tgctgttgtt tctccgtcac gcgcgttttt ccaaaactga
     3721 atgaagggca ctatatcgga tctgacccaa tgcgtgagaa aagttttcag cctgaccgcg
     3781 caatccgcgc gccaagcgcc aaatcaggaa agccagtcag gcatcaattt aagggtgatt
     3841 tcacttataa ttataagtga aatcaacgct gaaaaacacg atatttttaa agaaatataa
     3901 tatttataat tgacgtatca gcaataggat atagataatc atgttgaggt gtatccacat
     3961 ataataaaaa gtgaaatcgt gtcgcatttt tggggctggt ggacgtttgg cggtcttggc
     4021 gcgtagaatg ccaatacagt ttcgcctcgc ccgccttgca tctggggcag acttgtcgcg
     4081 tacattatcc tggttagggg agcgttgggg ggatatccag agtttataaa ctgtggatgg
     4141 acacggcggg gggtggggat tttgattggg ttggacgggg ggggaggggt ggggctataa
     4201 atgaggtatg gaacggcctg ttcaggcatc ggcaaaggtg gcagagcgcg cggctttttt
     4261 gcgcgcggag ctgacgcggc acaatcatct gtattacgtg gaggcgcagc cggaaatctc
     4321 ggaccgggaa ttcgaccggt tgctgcggga gttgcaggac ctggaggcgg ctcatccgga
     4381 gctgcaaacg gcggattcgc cgacgcagcg ggtggggggg gcgccgctga cgggattcca
     4441 gcaggtgaag catgcggtgc cgatgatgtc gctggacaac acgtacagcg cggaggagtt
     4501 gcgggagttc gatgggcggg tatgcaaggc gctgggggtg gaacgggtgg aatatgtggt
     4561 ggagccgaag gtggatgggg tgtccatcag cctgcgctac gagcacggcg tgctggtgca
     4621 ggcggcgacc cgcggcgacg gccggacagg ggacgacatc acggccaacg cacgcacgat
     4681 ccgctcgatt ccgctgcgga ttgaatcggc gacgccggtg ctggaggtgc gcggcgaggt
     4741 gtatttgggt catgcggcgt ttgcgcggct gaacgaggag cgggcggcgg ctggtgagcc
     4801 gttgtttgcc aatccgcgca acgccacggc aggttcgctg aagcaactgg acccgaaggt
     4861 ggtggcggcg cgtccgctgg ccgcggtgtt ttatgccacc ggcgaatgga cgggccctga
     4921 attcgccacg caggcggagt tgctgcgtgg attgaagcaa ttgggtttcc cggtggcggc
     4981 actgtggtgg gaatgccgcg acattgcggc agtgatcgag cgcgccatgg agctgcaaca
     5041 gcgcgagggc gagctggcct acgacatgga tggcgcggtg gtgaaggtga accgcctggc
     5101 gctctggcgg cagctcgggg ccacggccaa agcgccacgc tttgccatgg cgtacaaata
     5161 ctcgcgcgag caggcgcaaa ccatcctgcg cggcatcacg ctgcaagtgg ggcgcacggg
     5221 cgtgctgacg ccggtggcgg agcttgaacc ggtggagttg gccggctcga ccatcgcgcg
     5281 cgccacgctg cacaacgagg atgaagtgcg gcgcaaagac atccgggtgg gggatacggt
     5341 ggtgattgaa aaagcggggg aggtcatccc ggcggtggtg gcggtggtgc cggacttgcg
     5401 gccgccggga acgcagcctt ttgatttatt cgcccacgcc ggcggccgct gtccctcatg
     5461 cgggcaaccg atcagccgcg atccggcggc ggcggcgtgg cgctgcacca atgacgattg
     5521 ccccgcccag gtgcgcgggc ggttggagca tttcgtggcg cgcaaggcga tgaacattga
     5581 cggcctcggc gaggccattt tggccgcgct ggcttcgcca gtgacggtgc aggaaaaagt
     5641 ggacgacggc ctgttcggcc aaaaaatcga ggaacgggta ttgccgccgg tggtgcacga
     5701 cgtggcagat ctctacgccc tgacgccgga agacatcgaa ttgcggcggc ccaaccgcgc
     5761 gctggattcc aaggcggcaa gcatgaaact ggccaccaag ttgtgcgcag cggtggccgc
     5821 aagcaagaat aacgaactgt ggcgactgat tcacggcttg ggcattccga atgtgggcga
     5881 ggggttggcc cgatcgctgg cgcaggagtt gggctcgctg gatgcgctgc tggcggcatc
     5941 ggcggagacg ttggcgggcg tgcgcgatgt gggggacatc gtggcgcaaa gcgtggtgga
     6001 ctatttcgcg aatccgcgca accgggagac cgtggaaaaa ctgcgccggg cgggggtgcg
     6061 ctttgaccgt gtggagcggg cggcagcagg cgcgagcacg gacggatttt tcttcggcaa
     6121 gcgggtggtg attacgggca cgctggcaaa gttcacgcgg cagcaggcgc aggaagaatt
     6181 gcgacggcgc ggtgcgacgg tggcggaaac cgtcggcaag accacgcaga ttctggtggc
     6241 aggcgaagcg gcaggcagca aactggccaa agcccagaag ttgggcatca cgatcctgga
     6301 tgaagtcgcg ttcctggaaa aactgggcga aacgaacggc tgaaacttac gccgaagcgg
     6361 ggggcaattt atcggcgagc agcgcggcgc aagcgggcat gtccggtacg caataggtgg
     6421 cggcagtggc aggcgcggcg gggtccacca gtacggtgac cgcgagaccc gcgttatggc
     6481 cggtttgcac atcgctctcg cggtcgccaa tgatccagga gcgcgccaag tccagatgat
     6541 gccggcgggc agcatcgaag agcattccgg gattgggttt gcggttgggg tgggcggcat
     6601 cgtcggcggt gcaaatcagg atatcgagca gggcgaggcc ggcgcgggca agttcgtcgt
     6661 gcaggcgttg gtgcatggcg gccagttgat cgggcggggt gatgccgcgg ctgatgccgc
     6721 gctggttggt gacgatgacg gcgggccagc ctttggccgc agccgcgcgt acgcaatcgg
     6781 caaaaccggg gagaatgtgg aaatcatcga gatgattcac gtagccgggg ccggggctac
     6841 ggttgacaat gccgtcgcgg tcaaaaaaaa cgcacgggcg gggggcgggg gaggaattag
     6901 tagccggtgc cacggaaaat cacacccacg gttttcagca ggatttggaa atcgagaccc
     6961 agcgaccacg tatcaatata cttgagatcg aggcgcatcc attcctcgaa gggcaggtcg
     7021 ttacgcccgc cgacttgcca gagacaggtg cagccggcgc gcatggagag gcggcggcgc
     7081 tgccagtttt catatttggc gacctcggcg gggatggggg ggcgggggcc cacgagggac
     7141 atatcgccca cgaggacgtt ccacagttgg ggcagctcat cgagcgaata gcggcgcagc
     7201 cagcggccca cgggggtgat gcgcgggtcg tccttgatct tgaacacggg gccggtgact
     7261 tcgttgagct gttcgagttc agcgcgaatt tgctcggcgt tggcgaccat ggtgcggaac
     7321 ttgatcatgt tgaattctcg gccgcgcagg gtgctgcgcc gttgcgtaaa gaaaatgggg
     7381 ccgggcgagg tgaggcggat ggcgagcgcg ataccgagga gcagcggcga gagcacaatc
     7441 aggccgatgg ccgcaccgca gatatccagc aaccgcttga acaggagcgc ccacgaggat
     7501 ttggcggtgg tggtgaagag caggacggga tcgccggcca cttcatcgag cgtggtttca
     7561 gcaatggtgg tgtgtagata gggcggcagg agccatgctt cgatgccttc gatttcgcac
     7621 tggcgcagcc actgggtgac ttgcgcggtg gaaacatcgt cgggcagaat caccacggca
     7681 tccaccacgg tggaatgcag gatggcggaa aaatcgtcga gcgtgccggc ggcggggaaa
     7741 tcgggcggcg gagtcgtgcc ggcgggggag aggaagccga caatttcaaa aaagcgcgcg
     7801 cgggtgggat ggctgagcca ggcgcggatg ccggcggcgc gggcggggct gccaatgaac
     7861 agcaggcggc ggcgcgcttc cagccggagg atatagggtt gcagataaat ggcgcgcaag
     7921 gccacggcca agctggagta gatggcatga atgcccagga tggtgcgggg tacggcgtgc
     7981 agcttaaacg cgtaaagcaa gcccagcaaa accgcaaagc tcaagatgcc gccacgggcg
     8041 gcctgttgaa aaattcgggc gtgcggcagg ccgatgaatt ggcggtaaaa gccggagaaa
     8101 tccagcacga aaatccacac cggcaccacg agcacataga gccaggagtt ggaccaaaac
     8161 atgtcaaaaa tggcgaagta gccgggaaag agccacacca gcatcttgtg ggtgacaagc
     8221 gcggccagca gcgccccggc cacgagcagc gcatccacaa tggcggccag ttccgactgg
     8281 aatttgaaac ttctacgcag catggttttg cggagcgaag gataacaggc ggcggcggta
     8341 aaaccagcct aaaaacgctg caagtcttcg cgtagaaacg cgaggacatc cttgaagcgc
     8401 tgcaaatttt cgggatccac gcgggtgaga gtttcgtggg cgtcgtacat cagcgcggcc
     8461 atatcgcgct gacttaccgg ggcggcttcc accgggcgca agttttgctt caagccggag
     8521 agatcagcca agccggcggg cagctcggcg gcggaataac ttttgagcag gcggtccacg
     8581 cccagcccgc ggagcaggct gcgggcgtga ggtgtgggat tgagcagcgc gaggcggcaa
     8641 tcggaggtgc gctgggccag gccggcggtg gctagcgcgc ccatgaaggt gctatccatg
     8701 gaaacgcaat cggccatgtc caccaccacc agcgggctgc ccgccagttt ggccgcttgc
     8761 agcgcctgct tgaacgccgg cgccagcttg aacgtggctg cgccgctcac gacgatcagg
     8821 gtgcaattgt cggccgtggc cgccagaatc cgaggagatt ggggggcggc gcggctcatg
     8881 gttagctggt ccagttggcg gaagtttgcg tggcgagcac cttgccccgc cagactacgc
     8941 gcaaacgcca tttgatcacg cgtccgccct gccggacttt gtcgggcggg atttccagca
     9001 ccgagagtgt attgccctcg gacttgtccg tggtgcgcaa aaaatagctg cgcaccacag
     9061 gactgcgctc ttggacggtt tcgagcaaga gcacggtacc cggcggaatg ccggggggat
     9121 tggcgtacca acggatgtcg aaccgctcgc gcccgccggt ttcggagtcg gggtcttgga
     9181 aggcgcgtac gccggcgccg ggcacgctgg gggtggcttg gtacagaaac gattgctgta
     9241 ccccgatgat gtgggcgcgg gccacatagg tataaggctc ggcgcggaca accgccggca
     9301 tcagcagcat ggccaacgcc acgccggcac cccggcggac cacgcctcgc aatctgtcgg
     9361 cgcaccctac catggccggc caccctagca tccggaacaa cacggtgtca acgcgggcgc
     9421 ggttggaatg aagactcgcg ggcgagctgc tcgaacagcg ccttttcttt ggcggtggga
     9481 cgatcaggca ccacaatgcg gaccgtcacc agcatatcgc cgcgcgaccc cgtcttgttt
     9541 ggcaaccctt tgcctttcag acgcaagcgc gcgccgtttt gtgtgccggg tttgatgctc
     9601 agcgtggcgg gtccttccag cgtggggatg cccactttgg cgccgagcac ggcctcccag
     9661 ggcgcaatgg ccaccacttg gtccaagtcg tagccgttca actggaaaat cgggtggggt
     9721 gcaatccgca aattgagata caaattgccg ccggccgcgc cctgcccgga gaggcggatg
     9781 cgggtgccct cggtggcacc ggtgggaatc cgtacatgga acgttttggc ctcgtgcgcc
     9841 accgcgccgt tgggccgcac cacgggtacc tgcaaagtga tggccttggg ctcgcgattg
     9901 atcaaatcct ccagcgtgat cgccagcgtg gcctcaaaat cctggccacg cggctgaaag
     9961 gcttcttcgg gcgagccggc gccaaacccg ccaaagtcgg taaacggact gcttcggcgg
    10021 ctccgcccgc tacggcccgc cccggccaga tcgctgaaca gcgctgagaa gaaatcgctg
    10081 aaatctccgc cgccgctgtc gccaaactca aaacgaatgc cgcccggccc gctgctggcg
    10141 ccaccgaatc caccccatcc tggaggcggc gtgaagttct gcccggcctt ccagtttgcg
    10201 cccagcgcat cgtagcgctt gcgcttttcg ggatcggaga gcacctcgtt ggcttcgttg
    10261 atttccttga aacgggtctc ggcgccggcg gttttgttga catccggatg atactgccgc
    10321 gccaacttgc ggtaggcttt tttaatttcc tcggcggtgg ccgtgcgggg cacgccgaga
    10381 acggcataat aatcgagata cttcatgggg caggcggttt accggcggcg cttgaggtcc
    10441 tgcacatcct tttcgagatc atccaaacgg cgttccacat tatcgaacgg cgatggcgca
    10501 cggaatgtgc gccccaaccg cgcctcgacg gtgtccaacc ggtcttcgat tttgttcaaa
    10561 cgcgcggtca ggtcttcgat atcgtgctgc aggttccgga catcgcgggt gactgcgtcg
    10621 tccggcggtg attcggcgaa caccccgccg gccaacagca gcgacagcgt ggcggcaagc
    10681 atcatagttt tcatggcagc tccttctttg atgcggcaac tatacgccac cctcccgccc
    10741 gagggcaacg tccttgccgg gtcgccacca cgccgccacg cggcgccggc gggcgcaaaa
    10801 aaagaatggc gttcagttga cgcggggcgg ggggggctgg tatggtgcgc gagatttcat
    10861 accggggcac ccgtggtgaa attggcagac acgtaagatt caggttctta tgcccttaaa
    10921 agcgtgtggg ttcgagtccc tccgggtgca cca
//
LOCUS       DAOPNP010000085        10938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6854_length_10938_cov_10.906276, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000085 DAOPNP010000000
VERSION     DAOPNP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6854_length_10938_cov_10.906276"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKI64_08120"
     CDS             complement(<1..397)
                     /locus_tag="PKI64_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOE01011.1"
                     /translation="MRIHPFAALRPRPDIVGQVAAPPYDTLDTAEAAALAKDNPMSFL
                     HINHSEIDLPAGTNLYAEEVYAKAAENFANFAKQGWLVREDRPLMYLYAQTMGSHTQT
                     GLVCCAHIDDYVQDVIKKHEFTRKDKEDDR"
     gene            457..1680
                     /locus_tag="PKI64_08125"
     CDS             457..1680
                     /locus_tag="PKI64_08125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOE01012.1"
                     /translation="MTATKQDEAQPGPGLRVRLKPGREKPILRGSAWVFSGALDEVDG
                     AAPAGAPADVFDYAGDWLGRGLWHPTADLSVRLLTRDPHKEVNEAFFRRQIRQSVQRR
                     NRLFGPPESWQQTNAFRLVFSEADGLSGLIADQYAGTVVLQVGSKVWEPWLAVVRDEL
                     LAATGASGVVMRTDADAVKREGLPETLGGDAAPESVRIRENGLTFEVALAGQKTGFYL
                     DQRDNRRKVAAYARDRDVLSAYCYTGAFEVLAAHAGARSIRGWDSSAPALAQARRHHE
                     LNPHPVPVAYEEADVPQRLRLARDQRESWDLIILDPPRLVTRREGLDKGLRAYKDINL
                     LAIKLLRPGGILATFSCSGLVDMAEFRKAVGWAAQDAARAVRIVECLGQPADHPVPVE
                     MPECEYLKGLVCVVD"
     gene            1677..2543
                     /locus_tag="PKI64_08130"
     CDS             1677..2543
                     /locus_tag="PKI64_08130"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOE01013.1"
                     /translation="MSILLLTTLGWTGCGGPRHAPADATAQRVVCASPAVAEIVFALG
                     VGERVVGVSDFSDWPPEAKAKPRIGGALVPNREVILALQPNLILAQGRAEILHRFATA
                     QGIHFAAWPLDTLADLRAMIRGIATTLGEDEHGRQLVARLDADFAALAPERMTPVFIA
                     LSHAPGELAGLMTAGPHTFLSELVELAGGQNIFADVTASWPHISQETLVRRRPELILD
                     FQPTQLSDAQRTALLADWGKLGFSPEQVRILEEDYLLKPGPRAPVAAGIIAAALQPLT
                     SGMGQRNAPVSR"
     gene            complement(2492..3499)
                     /locus_tag="PKI64_08135"
     CDS             complement(2492..3499)
                     /locus_tag="PKI64_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE01014.1"
                     /translation="MTAASSGVCELFFYEAFAEEAERLRHYAAAASVRVGHTPLTIQE
                     AGHTAPPAPVISVRTQSVLPLDWAAQLRGILSRSTGYDHLTVYRAAAGESCPQLGYLP
                     LYCARAVAEQAMLLWTALLRHLPRQVAQFATFHRDSLTGWESQGKTIALFGVGNIGYE
                     IWRIATALGMTVRGVDPVQRHADVTYYSPADALAAADIIVCAMNLTPANHRYFTRVVL
                     AQAPRRPILVNIARGEFTPATVLEAALNDGLLSGVALDVFNEEATLAPALRANNPGMF
                     TADNQALLRLHQRPEVILTPHNAFNTAEAVERKSEQSLRAFLHYRETGAFLWPIPDVN
                     G"
     gene            complement(3501..4514)
                     /locus_tag="PKI64_08140"
     CDS             complement(3501..4514)
                     /locus_tag="PKI64_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01015.1"
                     /translation="MTDPTPDTPDVLPPEPAGALSVPPPIPQKKPGFFRRLFTSRKKQ
                     QAVAMQNGYLEMVDLIRAIRAHLDRQEVVQTSVLTMLEKVPGAMDRQHEVMTMFKQQL
                     ESGMENNRRLTESMGNLNNTLASMDESQRASSRTITDLIHRSRESEQLLREVMRRSER
                     RMTLLVVFFFVAVVGLGFYFSHGQARAPKVKAPVPSVVTEPARSAKPAKVEPVKDAPK
                     KVEPAKAEPKPSPLVAPPVVKEQPAKDQPTQPAAKPEKAAEKPAEKAKPQADKPAKTD
                     KADKPAKKAKREPAAAPEPAPTAAEAPPAAEPEAAVGQPDLTLEQLRPAISPAADRPV
                     SLP"
     gene            complement(4618..5358)
                     /locus_tag="PKI64_08145"
     CDS             complement(4618..5358)
                     /locus_tag="PKI64_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOE01016.1"
                     /translation="MAARGASPAVIPTVRNILRDLAQQKNPAQAKHLRRFFRTGPGEY
                     GAGDQFWGLKVPQTRAILGRYGPIPLPVAAELLAHPVHEARFFAAIALARDYAKATPA
                     ARVAIFNFYLAHARRINNWDLVDLSAPNIVGVHLPAGGGRQVLARLARSTNMWERRIA
                     IVATYTHIRQGDLRNTFWLAERLLNDHEDLLHKAAGWMLREAGKRDPAALRDFLQRFA
                     PRMPRTMLRYAIEKFPPAERRAWLTFGK"
     gene            complement(5362..6015)
                     /locus_tag="PKI64_08150"
     CDS             complement(5362..6015)
                     /locus_tag="PKI64_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01017.1"
                     /translation="MKTAWIALLATALLTGCETGTMDNQGTVTLNELQGIPEPTPEPE
                     TPPAEQGNTNSQTQTNSPSDGGSTGSTSTTPDGGTTSNEGFPSEIDTPIKWLHTDVSR
                     WPVTADISVSVSGGKVNFPYSKSRSWPAVDGVNANPWVFAKKDGQWYAATFEWLRFGQ
                     TAKPTYTVRGDHVKVAPLNGSWAPKSGERIGIMVSGLARHKQRNVQERSRVVMITWP"
     gene            complement(6163..7143)
                     /locus_tag="PKI64_08155"
     CDS             complement(6163..7143)
                     /locus_tag="PKI64_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOE01018.1"
                     /translation="MTTPSHFTVQDFADFCARNRPAYHAGYYAMYSSWWNAITTDPNL
                     MVVPVDDHMVHRGDGLFETFKCVDGAIYNLDAHLARLENGCRAIGLHLRWSRAEITRL
                     TGEVLRAGGHRDALVRILVSRGPGGHTANPYECPEPLLMILAIRLPPPFMTAHPAGAS
                     VGVSSVPPKGMFFAQVKSVNYLPNVLMKKQAVDAGVDFMLGFDANGNMTELPTENFGI
                     VTRERVLRVPKPDHILQGTTMARVLELARRELVPAGILAGVEEADVPRAQLADAAEML
                     VFGTTPNVTAVTQFDGRPVGDGQPGPVWRELSRLFEADLRDPQRLTPLFD"
     gene            complement(7158..8606)
                     /gene="rpoN"
                     /locus_tag="PKI64_08160"
     CDS             complement(7158..8606)
                     /gene="rpoN"
                     /locus_tag="PKI64_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE01019.1"
                     /translation="MKQGLHQQQKQRQEMKMAPRMIQSLRFLQSPLPELHELVRAELE
                     QNPVLEEKLGGDELPLAAPEPLTAAAYDDVDQENDFDSDDYAREIDALERMLAERPTD
                     GLPGPPAPSPEAEQKRQYFLDSITAPPSLHAHLMDQLNEAGMSAAQKKAGEYIIGNVD
                     ENGWLAADVAEIAREAAVTPDDVLAALAIVQEFDPPGVAARSLQECLLLQLRRTGHAP
                     DSLPGQLVVSHLAELRTQSAAELAKAGGWAEADTAAAMQTVLALDPKPGVKFSAPPPV
                     YVTPEVEIRPTPEGGYAVDMLRDGLPRLHLSGEYTEMLQDPTTAADAKKFLQEKLRDG
                     QFVLDSLHQRRQTIRRIAEEIAAAQQEFFAHGISKLKPLTMGALAAKLGLHETTVGRA
                     VSGKYVRTPQGVFELKFFFTPSLKTADGGAVSTVAVREAVRRLIAREDIRKPLSDQAI
                     ADALGAQGLQVARRTVAKYREELGLPPAHKRK"
     gene            complement(8685..9683)
                     /locus_tag="PKI64_08165"
     CDS             complement(8685..9683)
                     /locus_tag="PKI64_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOE01020.1"
                     /translation="MLAPMAGYTGGAFRSLCLEQGCGAVVTEMVSAQGLIRHHARTKH
                     YLDRFGDEQPIGAQIYGSDPGAMAAAAAMVAERNEFVFLDINAGCPMPKIRNRGDGAG
                     LMRDPARLFEIVRQVCGAVGGKMPVTVKTRIGWSADTINVMETTAGVEEAGAAAIFLH
                     GRVASVRHSGPVNWPLLAEVKRARRIPVLGNGGIQTATDVFHMIRETGVDGAMIGRAA
                     LGNPWLFHDIRELAAGRSSSVPSVEEVRGMIFTHLDRELAMMARRGSRQLRLGAELTA
                     CLVMRPHLVRYLRGFRGFGVLARTLEERVDAATLRQRIDAVLATGRRCEPPSDKAS"
     gene            complement(10055..10804)
                     /locus_tag="PKI64_08170"
     CDS             complement(10055..10804)
                     /locus_tag="PKI64_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE01021.1"
                     /translation="MSSSYSIGHVFGIPVRVHITLLIFLPFFAFSFMPVDGLIGLWYG
                     AIGAVGLFGSVVLHEVGHSLVARAKGAHILEILLLPIGGMARLSQMPRRPMDEIQTAL
                     AGPAVSLALGGLGWFGAPWVYPFNPLLAVMIDALGRINFMLVLFNLIPSFPMDGGRVF
                     RAALVPRLGRLAATQLAAKVGRAFAWLFAIWAIWPLFHGDSIRITLLLIAYFIHQAAT
                     AEARVAEMEAWADHQRAQSSGLFDDLLHKWR"
BASE COUNT         2143 a         3335 c         3303 g         2157 t
ORIGIN      
        1 tgcggtcgtc ttccttgtcc ttgcgcgtga attcgtgctt tttgatgacg tcctgcacgt
       61 aatcatcaat gtgcgcgcag cagaccaagc ccgtttgggt gtgcgaaccc atggtctggg
      121 catagagata catcagcggg cggtcttccc gcaccaacca gccctgcttg gcaaagttgg
      181 caaaattttc cgcggccttg gcatagactt cttcggcgta gaggttggtg ccggcgggca
      241 aatcaatctc cgagtggttg atgtgcagaa acgacatcgg attatccttg gccagcgcgg
      301 cggcttcggc cgtgtccaat gtgtcgtacg gcggcgcggc cacttggcca acaatgtcgg
      361 ggcgggggcg gagagcggca aatggatgaa tacgcatggg atacctcttt ttccggtttg
      421 acctgttatc tgaatgggct gactatccta atgaatatga ccgcgacaaa gcaagatgaa
      481 gcacagccgg gccccggctt gcgggtacgg ctgaaacccg gccgcgaaaa accgattttg
      541 cgcggcagcg cgtgggtctt ttccggcgcg ctggatgaag tggacggtgc cgcaccggcc
      601 ggagccccgg ctgacgtttt tgattacgcc ggcgattggc tcgggcgcgg cttgtggcac
      661 cccaccgccg acttgtccgt gcgcctgctc acccgcgatc cgcacaagga agtgaacgag
      721 gcctttttcc ggcggcaaat ccgccagtcc gtgcaacggc gaaaccggtt gttcggcccg
      781 ccggaatcgt ggcaacaaac caacgcgttc cggttggttt tctcggaagc cgatggcctc
      841 tccgggctga ttgccgatca atatgccggc acggtggtgc tgcaagtggg cagcaaggtg
      901 tgggaaccgt ggctagccgt ggttcgtgac gagttgctcg cagcgaccgg cgcgagcggg
      961 gtggttatgc gcacggatgc cgatgccgtc aaacgcgagg gcctgccgga aacacttggc
     1021 ggcgatgccg cgcccgaaag cgtgcgcatc cgtgaaaatg ggctgacgtt tgaagtcgcg
     1081 ctggccggcc aaaaaacggg attctatctc gaccagcgcg acaaccgccg caaggtggcg
     1141 gcctatgccc gcgaccgcga tgtgctgagc gcctattgct acaccggcgc attcgaagtg
     1201 ctggcggccc atgccggcgc caggagcatc cgcggctggg attcatccgc gccggcgctc
     1261 gcgcaggccc gacggcatca tgaactcaac ccgcacccgg ttcctgtggc gtatgaagaa
     1321 gcggatgtgc cgcagcgcct gcgcctcgcg cgcgatcagc gcgaaagctg ggatttgatc
     1381 attctggacc cgccccgcct ggttacccgc cgcgagggcc tcgacaaagg actgcgcgcc
     1441 tacaaggaca tcaacctgct ggcgatcaag ctgctgcggc cgggcgggat tttggccact
     1501 ttctcctgct caggcctggt ggacatggca gaatttcgca aagcagtggg ctgggccgca
     1561 caggatgccg cccgcgcggt tcggattgtg gaatgccttg gccaacccgc ggatcaccct
     1621 gtgccagtgg agatgcccga gtgcgaatat ctgaaggggc tggtgtgcgt ggtggattga
     1681 gcatcttgct gctcaccacg ctgggctgga cgggttgcgg cgggccgcgc catgcgccgg
     1741 ccgatgccac cgcgcaacgc gtggtttgcg caagtccggc ggtggcggaa attgtctttg
     1801 cactcggcgt cggcgagcgc gtcgtggggg tgagcgattt ttccgactgg ccgccagaag
     1861 ccaaagccaa gccgcgcatt ggcggcgcgc tggtgccaaa ccgcgaggtg attttggcgc
     1921 tccagcccaa tttgattttg gcccaaggcc gggccgaaat tttgcaccgt tttgcaacgg
     1981 cgcaaggaat tcattttgcg gcatggccgc tggatacgct cgccgatcta cgcgccatga
     2041 tccgcggcat tgcgacaacg ctgggcgagg atgaacatgg gcgccagctt gtggcgcggt
     2101 tggatgctga ttttgccgcg ctggcaccgg agcggatgac gccggtgttt atcgcgttga
     2161 gccacgcccc cggtgagttg gcgggactga tgactgccgg cccccatacg tttttgagcg
     2221 agttggtcga gctggccggc ggacaaaata tctttgcgga tgtcactgcc agttggccgc
     2281 acatttcgca ggaaaccttg gtgcgccgcc ggccggagct tattttggat ttccagccga
     2341 cccaattaag cgacgcacaa cggacggcct tgttggccga ttggggcaag ctcggctttt
     2401 ccccggaaca ggtgcggatt ctggaggaag actatctgct caagccaggc ccgagggctc
     2461 ccgtggcggc gggcatcatc gcggcagcgc ttcagccgtt gacgtcgggg atgggccaga
     2521 gaaacgcgcc cgtctcgcga tagtgtaaaa atgcgcgcag cgattgctcg gatttgcgct
     2581 caacggcttc ggccgtgttg aaggcgttgt gcggcgtcag gatgacttcc gggcgctggt
     2641 gcaaccgcag caacgcctga ttgtcggcgg taaacatgcc cggattgttt gcgcggagcg
     2701 ctggcgccaa cgtggcttcc tcattgaaaa catccagcgc cacgcctgag agcaggccgt
     2761 cgttgagcgc ggcttccaac actgtcgcag gcgtgaattc accgcgcgcg atattgacca
     2821 agatggggcg gcgcggtgcc tgcgccagca ccacgcgcgt gaaatatcta tgattggcgg
     2881 gggtcagatt catcgcgcac acaatgatgt ccgccgccgc aagcgcatcg gcgggtgaat
     2941 aataagtcac gtcggcatga cgttgcacgg gatccacgcc acgcacggtc attcccaacg
     3001 cggtggcgat gcgccagatt tcatagccga tgttgcccac accaaacagc gcgatggttt
     3061 tgccttggct ttcccagccg gtcaggctgt cgcggtggaa tgtggcaaat tgcgcgactt
     3121 gccgcggcag atgccgaagc agtgcggtcc ataggagcat ggcctgttcc gcaaccgcac
     3181 gcgcgcagta gagcggcaga tagcccagtt gtgggcaact ttctccagcg gcagcgcgat
     3241 agaccgtcaa atggtcatag ccggtggagc gtgacagtat tccgcggagt tgtgcagccc
     3301 agtccaacgg caaaacagat tgggtgcgca cgctgatgac tggcgcgggc ggtgcggtgt
     3361 ggccggcttc ttggatggtc aatggcgtgt gccccacgcg tacgctggca gccgccgcgt
     3421 aatgccggag acgctccgct tcctccgcga acgcttcgta gaaaaatagt tcgcagacgc
     3481 cggatgatgc ggcggtcatg ttaagggagg gaaaccggac gatccgccgc cggcgaaatt
     3541 gccggacgca gttgttccaa cgttagatcc ggctggccaa ccgcagcttc tggctccgca
     3601 gcgggaggcg cctccgcagc agtcggagcg ggttccggcg cagccgctgg ctcgcgcttg
     3661 gctttcttgg ccggcttatc cgccttgtcg gttttcgccg gtttatccgc ttggggtttg
     3721 gctttttccg ctggcttttc cgctgctttt tccggcttgg ctgccggctg cgtaggctgg
     3781 tccttagccg gttgttcttt cacaacaggt ggtgcaacca gtggcgaggg tttgggctcc
     3841 gccttggccg gctccacttt tttcggtgca tctttgacgg gttccacttt cgcgggtttg
     3901 gcggatcggg ccggctcagt caccacgctg ggcacaggcg ctttgacttt gggcgcgcgg
     3961 gcttgtccgt gcgagaagta gaagcccaac ccgaccaccg ccacaaagaa aaagacgacc
     4021 agcagcgtca tccggcgctc ggagcgacgc atgacttcgc ggagaagttg ttcggattcg
     4081 cgcgagcggt gaatcaaatc ggtgatcgtt cgcgaggacg cgcgctgcga ttcgtccatc
     4141 gaggccagcg tattgttgag attgcccatg ctctcggtaa gccgccggtt gttttccatc
     4201 ccgctttcca actgctgctt gaacatggtc atcacttcgt gctggcggtc catggcgccg
     4261 ggaactttct ccagcatggt cagcacggac gtttgcacca cttcctggcg atcaagatgc
     4321 gcgcggatgg cacgaatcaa gtccaccatt tccaggtaac cgttttgcat ggccacagcc
     4381 tgctgctttt tccgcgacgt gaacaagcgg cggaaaaatc cgggtttctt ttgaggtata
     4441 ggtggcggaa cggatagcgc gccggcaggc tccgggggca ggacatcggg ggtatcgggg
     4501 gtcgggtcag tcatggtgat gctcctccgc aagaatagtt cacgctcgat atggtatgct
     4561 ggatttgccg ccgcccgcaa tccaaaagag gaggaggcgc gggccgcgcg gatgcggtca
     4621 tttgccgaac gtcagccaag cgcggcgttc cgcgggcgga aacttttcga tggcatagcg
     4681 cagcatggtg cgcggcattc ggggcgcaaa gcgttgcaga aaatcgcgca aggcggcggg
     4741 gtcgcgcttg ccggcttcgc gcagcatcca gcctgcggct ttgtgcagta aatcttcgtg
     4801 atcgttcagc aagcgttccg ccagccaaaa tgtattgcgc aaatcgccct gccggatgtg
     4861 cgtataggtg gccacgatgg caatccgtcg ctcccacata ttcgtggagc gagccaagcg
     4921 cgccagcacc tgccgcccgc cacctgcggg cagatgcacg cccacgatat tcggcgcgct
     4981 caaatccacc aaatcccaat tgttgatgcg acgggcgtgc gccaaataaa aattaaaaat
     5041 cgctacacgc gcggcgggcg ttgccttggc gtaatcgcgg gccaacgcaa tggccgcaaa
     5101 aaatcgtgct tcgtgcacag gatgggcgag caactcagcg gcaacgggca acggtatcgg
     5161 gccgtagcgc cccagaatcg cgcgggtttg cggaactttc agaccccaga attgatcgcc
     5221 cgccccgtac tcccccggac ccgtccgaaa aaatcggcgc aggtgtttgg cctgcgccgg
     5281 atttttttgt tgggccagat cgcggagaat gttccgtacc gttgggatta cggccggaga
     5341 tgctccgcgt gcggccatgc tttacggcca ggtgatcatc accacgcggc tgcgttcctg
     5401 cacattgcgc tgtttgtggc gagccagtcc gctgaccatg attccgatcc gctccccact
     5461 cttgggcgcc caactgccgt tcagtggggc caccttgacg tggtcgccgc gcaccgtgta
     5521 ggtgggcttg gcggtttgac cgaagcgaag ccactcgaat gtcgccgcat accattgacc
     5581 atctttcttg gcgaacaccc acgggttggc gttcacacca tccacggccg gccacgagcg
     5641 agacttgcta tacgggaagt taaccttgcc gccgctgacg ctgacgctga tgtccgccgt
     5701 gacgggccag cgcgaaacgt cggtgtgcag ccacttgatc ggcgtatcaa tttccgaagg
     5761 gaatccttcg ttcgatgtcg tgccgccgtc gggcgtagtg gatgtggagc ccgtgctgcc
     5821 gccgtcggaa ggcgaattgg tttgcgtctg gctgttggta ttgccctgct cagcaggcgg
     5881 agtttccggc tccggcgtcg gttccggaat cccctgcaat tcgttcaaag tcaccgtgcc
     5941 ttgattatcc attgtgccgg tttcgcagcc cgtcagcaaa gcggtagcca gcagcgcgat
     6001 ccaggcggtc ttcaatatca tttcaatccg tcgcatcgaa atctctccta tcctttgttg
     6061 atggtttgcc cgtaggtact aaagggcacc gatgatgaca acctaaatcg caccaagttt
     6121 tttgcgccgc aacggggcac agcgaaaagc gactccattg attcaatcaa agaggggagt
     6181 cagccgttgc ggatcgcgca aatcggcctc gaagaggcgg ctcaattccc gccaaacagg
     6241 gccgggttga ccatcgccga ctggccggcc gtcaaactgc gtgacggccg tcacattcgg
     6301 cgtggtgccg aacaccagca tctcggcggc atccgccaac tgggcgcgcg gcacatcggc
     6361 ttcttccacg ccggccagga ttccggcagg caccaattcg cgacgcgcca gttccagcac
     6421 ccgcgccatg gtggtgccct gcaagatatg atccggcttg ggcacccgca gcacacgctc
     6481 gcgagtgacg atgccaaagt tttcagtggg cagctcggtc atgttgccgt tggcatcgaa
     6541 ccccagcatg aaatccacac cggcatccac cgcctgtttt ttcatcagga cgttgggcaa
     6601 ataattcacg cttttgacct gcgcaaaaaa cattcccttg ggcggcacgc tgctcacccc
     6661 cacgctggcg cccgccgggt gcgccgtcat gaatggaggg ggcaggcgga tcgccagaat
     6721 catcagcaac ggttccggac attcgtacgg attcgcggtg tgcccgccgg ggccgcgcga
     6781 caccaaaatc cgcaccaacg cgtcgcggtg cccgcccgcg cgcagcacct cgccggtcag
     6841 ccgggtgatt tccgcacggc tccaccgcag atgaagaccg atggcgcggc agccattttc
     6901 caagcgcgcg agatgcgcat ccaaattata gatcgccccg tccacacatt tgaatgtctc
     6961 gaacagaccg tcgccgcggt gcaccatgtg gtcatccacc ggcaccacca tcaggttggg
     7021 gtccgtggtg atggcattcc accaacttga atacatggcg tagtagcccg cgtggtaggc
     7081 cggccggtta cgcgcgcaaa aatctgcgaa gtcctgcacc gtgaaatggg atggagtggt
     7141 catgaaactc tttcggttta tttccgtttg tgggcgggcg gcaggcccag ttcttcgcga
     7201 tatttcgcca cggtgcgccg cgcaacctgc aacccttgtg cacccaatgc gtccgcgatg
     7261 gcctgatcgg aaagcggctt gcggatatct tcgcgcgcga tgagccgccg gacagcctcc
     7321 cgcaccgcca ccgtggacac cgcgccaccg tcggctgttt tcaggctcgg ggtgaaaaag
     7381 aatttcaact cgaacacccc ttgcggagtg cgcacgtatt tgccggacac cgcgcgccca
     7441 acggtggtct cgtgcaagcc gagtttggct gccagcgcgc ccatggtcag cggcttcagc
     7501 ttggaaatgc cgtgcgcaaa aaattcctgc tgtgccgcgg cgatttcctc cgcgatgcgc
     7561 cggatggttt gccggcgttg atgcaggcta tccagcacaa attgtccatc gcgcaatttt
     7621 tcctgcaaaa actttttggc atcggccgcc gtggtggggt cttgcaacat ctcggtgtat
     7681 tcgccgctca aatgcagccg tggcaaacca tcacgcaaca tatccaccgc gtacccgcct
     7741 tcgggcgtag ggcgaatttc cacctccggg gtaacataca ccggtggcgg agcggaaaac
     7801 ttaacgcccg gcttgggatc caaagccagc accgtttgca tggccgcggc ggtgtcggcc
     7861 tccgcccaac cgcccgcctt ggccagctcg gcggcgcttt gtgtgcgcag ctcggccaaa
     7921 tgtgaaacaa ccaattgccc cggcaacgag tccggcgcat ggccggtgcg gcgtagttgt
     7981 agcaacaagc attcctggag cgaacgtgcg gccacgccgg gcggatcaaa ttcctgcacg
     8041 atggccaacg ctgccaacac atcgtctggc gtaaccgctg cttcgcgcgc aatctccgct
     8101 acatccgccg ccaaccagcc gttttcatcc acgttgccga tgatgtattc cccggctttc
     8161 ttttgcgcgg cggacatgcc cgcttcgttc agttggtcca tcaaatgcgc gtgcagcgag
     8221 ggtggcgccg tgatggaatc caaaaaatat tggcgttttt gttcagcctc tggcgagggt
     8281 gccggcgggc ccggcaggcc gtccgttggc cgctcggcca gcattcgttc cagcgcgtct
     8341 atttcgcggg cgtagtcatc ggaatcaaaa tcgttttcct gatccacatc gtcatacgcg
     8401 gccgcagtca atggctccgg tgcagccagc ggcagctcat cgccgcccaa tttttcctcc
     8461 aaaaccgggt tttgctccag ctcggcgcgc accagctcat gcagctccgg cagcggcgac
     8521 tgcaaaaaac gcagcgattg aatcatccgc ggcgccattt tcatttcctg gcgctgcttc
     8581 tgctgttggt gcaaaccctg tttcatgatc cccgccagtg tagcgcaatc ccgtcgccgg
     8641 gcaaaacgca atccgcacgc tgcgagctac gcgcgggcac ccctttaaga cgctttatcc
     8701 gatggcggtt cgcaacgccg tcccgtcgcc agcacggcat caatccgctg gcgcagcgtc
     8761 gcggcatcca cccgttcctc cagtgtacgc gctaaaacgc cgaaaccgcg aaagccccgc
     8821 aaatagcgca ccaaatgtgg acgcatcacc aggcaagctg tcaactccgc tcccaaccgc
     8881 agttgacgcg acccgcgccg cgccatcatg gctagttccc gatccaggtg ggtaaaaatc
     8941 atcccgcgca cttcttccac gctgggcacg ctcgatgatc gcccggcggc caactcgcga
     9001 atatcatgga acagccacgg attccccagc gccgcgcggc cgatcatggc gccatccacc
     9061 ccagtttccc gaatcatgtg gaaaacatct gtcgccgttt gaatgccgcc gttgcccaac
     9121 acgggaatac gccgtgctcg tttgacctcc gccagcagcg gccaattgac cggccccgag
     9181 tggcgtacgc ttgccacccg cccatgcaaa aaaatggctg ccgcgccggc ctcttccact
     9241 cctgccgtgg tttccatcac attgatggtg tccgcgctcc agccgatgcg ggttttgacg
     9301 gtcactggca ttttcccacc cacggcaccg caaacttgcc gtacaatttc aaacagccgc
     9361 gccgggtcgc gcatcaaccc cgcgccatcg ccccgattgc ggattttcgg catcgggcaa
     9421 cccgcattga tgtccaaaaa aacaaattcg ttgcgttccg ccaccatcgc tgccgccgcc
     9481 gccatggccc ccgggtccga gccataaatc tgcgcgccga tgggctgctc atcaccaaag
     9541 cggtccagat agtgctttgt ccgcgcatga tgccgaatca ggccctgcgc gctcaccatt
     9601 tcggtcacca ccgcgccaca gccctgctcc agacaaagcg accggaacgc tccccccgta
     9661 taaccggcca ttggtgcgag cactaccggc cactccaacc ggaacgaacc catctgaaca
     9721 gcctgtaagg gaaacccaat catgtccggc actctacgca cttttcaccg caatgggaaa
     9781 aaatattcgc cggccccctg ccggccgcaa tgggagccat acagaggaaa aaagcagctc
     9841 gatttgacat ataataaaaa gtgaaatatg cccccagaaa acggctattt ttatatatac
     9901 ataataattt ataactgatt gtttacaaaa tggttataat attttcatct ctaatcaatt
     9961 tcacttataa ttataagtga aatcagggtt gatttattaa ggttttaaag gtgtagcgaa
    10021 aagaggcggt tttttgccgt attaggcagg aaaatcagcg ccacttatgg agcaggtcgt
    10081 cgaaaaggcc ggaagactgg gcgcgctggt ggtcggccca agcttccatt tcggcgacgc
    10141 gagcttcggc ggtggcggct tggtgaatga aataggcgat gagcaggagc gtgatgcgaa
    10201 tgctgtcgcc atggaacagt ggccaaatgg cccaaatggc gaaaagccaa gcaaacgccc
    10261 ggccgacttt ggcggcgagt tgcgtggcgg ccagccggcc cagtcgcggc acaagggctg
    10321 cgcgaaaaac ccgaccgcca tccatcggga aagaaggaat caagttgaac agcaccagca
    10381 tgaaattgat gcggcccaac gcatcaatca ttacggccag cagaggattg aatgggtaca
    10441 cccacggggc accaaaccaa ccaagccccc ctagcgccag actgacagcc gggccggcta
    10501 gcgctgtttg gatttcatcc atcgggcggc ggggcatctg gctcaaacgg gccatgccgc
    10561 caatgggcaa gagcaggatt tccaaaatgt gcgcgccttt ggcgcgggcc accaatgaat
    10621 ggccaacttc gtgcaacacg acgctaccga agagcccgac ggcaccaatc gcgccatacc
    10681 aaaggccaat cagaccatcc accggcatga atgaaaaggc aaagaacggc aggaaaatca
    10741 gcagggtaat gtgtacccga actggaatgc caaacacgtg gccgatggaa taggatgagg
    10801 acatagcgaa caggcccacc ataacacagg gccggcacaa aggggagcca cttacgtaaa
    10861 gttgctcccc ttggctcgcg cgaggtgaat tgacaaagta actgcgcccc caaggggagg
    10921 gaaggggcgc agttagtc
//
LOCUS       DAOPNP010000086        10775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_6979_length_10775_cov_9.349254, whole genome shotgun sequence.
ACCESSION   DAOPNP010000086 DAOPNP010000000
VERSION     DAOPNP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6979_length_10775_cov_9.349254"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKI64_08175"
     CDS             <1..473
                     /locus_tag="PKI64_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE01022.1"
                     /translation="LGRFLTEIWPKILEKHPDARLRVAGHGLPAEQREKWRKIRQVEL
                     LGFVSDLVAEYNQCRAVIVPIYTGSGTNIKVLEAMQMGRPCVITNFAAKGFETLLQDG
                     KNILIAQNDAEFAHKLQKLLENPDFSAQIAHSAQSDIQTHLNAISYSAILSKFL"
     gene            1079..1873
                     /gene="ppk2"
                     /locus_tag="PKI64_08180"
     CDS             1079..1873
                     /gene="ppk2"
                     /locus_tag="PKI64_08180"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOE01023.1"
                     /translation="MKESAEKKGGKLKYRKELAKLQVELVKLQEWIKQKGLKVVVIFE
                     GRDAAGKGGVIKRITECLNPRICRVVALGVPTDKEKTQWYFQRYVAQLPAAGEMVLFD
                     RSWYNRAGVERVMGFCTDAEYEEFLRSCPDFERMLVRSGIILIKYWFSVSDEEQERRF
                     QARIDSPTKRWKLSPMDLESRARWVEYSKAKDKMFEVCDIAQAPWNVVHADCKKRARL
                     NCIHHLLSQIPYKDLTPKPMKLPPRQKRKGYVRPPLSDQHFVPEVY"
     gene            2003..2078
                     /locus_tag="PKI64_08185"
     tRNA            2003..2078
                     /locus_tag="PKI64_08185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2037..2039,aa:Glu,seq:ttc)
     gene            2115..2309
                     /locus_tag="PKI64_08190"
     CDS             2115..2309
                     /locus_tag="PKI64_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01024.1"
                     /translation="MSRNVRNNDTTRPRKSGAAKNRRLLEHRRRLVALGVDEQKVLQM
                     PHDRIRLLIAKPNLIKRYVQ"
     gene            complement(2382..3722)
                     /locus_tag="PKI64_08195"
     CDS             complement(2382..3722)
                     /locus_tag="PKI64_08195"
                     /inference="COORDINATES: protein motif:HMM:NF023375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3466 family protein"
                     /protein_id="HOE01025.1"
                     /translation="MWWFFVVGLLLAVHANAAAEYTVTDLGTLGGNHAEANGLNNRGQ
                     VVGWSLDADGRHQAFVWQNGRMTGLGFLPGGTSSVAMAINNGGKITGHAHISAANQRA
                     FLYVSNSMINIGTWGSINSMGRAINDQGAISGTAPPTNNSSYNSSFYWQTNQYTQIPA
                     FAPGNSCDAYGINNLGQVCGITFVYSPNPRWWAYVWHDDNTNGLHDNGEMKLLGTLGS
                     KDSVGEVSAANAINDHGLVVGWSAISNGYYPRHAFLITPSNGIWKLPSYSNTDPTNLL
                     MRSLGTLGGPTNNSSANALNNHGWIVGTSDTPAGTNQAFLWRNETMLNLNSLIPPDTG
                     WVLTNATAINDANEIAGNGLFNGEPRAFLLRREGRIHEFESHVLHWSGIWSTNINETN
                     VFTVEYSATLQPDSWQPAPPTSQWPIVDMFWSDDILPPASTRFFRVQARPFPGE"
     gene            complement(3779..8749)
                     /locus_tag="PKI64_08200"
     CDS             complement(3779..8749)
                     /locus_tag="PKI64_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE01026.1"
                     /translation="MKRALSSRQLLIAAGLMFLATLWWSHPRLNLAWSPPGPTAVEAN
                     QQAHAAIQQARAQTRTPPPAPLGDRPVIRLRAGDLFPGPLDEQPHHTPSTRGYPWLVL
                     FDGPLQPEWRQALQDAGATVRAYLPHHALLVEAPAATIRRLAHMPHVAWHGEYRPAYK
                     LQPLLAALLKKTPAIALPVTIQTFAPDDAFAVAAVLRAAQAGEVSATPGKRWGLVRAV
                     VPAGEIAALAARADVQWIEHRPTPRLLNDVARAANRLNVDAAHDDHGLDGAGQIVAVA
                     DTGLDSGNLASLHPDFVGQVLQVFDTGRGNDWSDKYYHGTHVIGSLLGTGAASTNQFR
                     GVAPSAQLVLQSVMDASSRLNLPDDLNELYQPAYDAGARLHSDSWGGAAEGEYNSDSM
                     TTDEFVWDHPDLFVAFAAGNEGTDDNRDGVVDEQSLDAPASAKNVLAVGASESGRPAG
                     SGGQTAQNYGMNYPSRFRTEPILSDLISSSPGGGPQGISALSSRGPAMDGRTKPDVVA
                     PGSDVVSARSRASSSTGRGVLSGNTNYCFMTGTSMAAPFATGCATLLRQFCVETRGLE
                     NPSAALLKAALVGGARSLSPGQYGTNEFREIPAPPRPNSVEGFGQINLAGTLFPTGAT
                     QVALLDSPAPLTTGGSNTLEFIVRESAPLTATLAYSDYPSALAAAVNLVNDLDLALHD
                     PDGASYFPNGLAEPDRLNNVEGIDLDAPVTGRWTLVVSAHNVPEGPQPYAIYMRGAID
                     LPAAIEHAPLDNTWQTNVEYTVTARVTSPGQLDPDTVQLHWNTTGSTDDFNVVTMTPG
                     SNQMFSAAIPAQPTDTWVYYYLSAGPADLLTTHPPEAPAALHQFAVTQPLTLTITGAP
                     GEYFAVNPGYGTHTFASGIVVQADAAFSATGTNGMRTACVGWQGVGFVPAAGVANTCE
                     FTITADSSLVWQWQEQALLMHISEPHGILNTNVWHELGSAASSLPAPESALVGQTPYT
                     FAGWLVDGARWPTNGEPSQHQVDGIPMTEPRAATATYLPTEQDSDANGLPDWFELRYF
                     GALGQSRFADPDGDGHENEVEAADHTDPLDSASVPVAPGITHEPLASPVASPAPWLLS
                     AIITDNYRVATATLHWQRNGGLPRSAPMTNSAGDVFTGAIPSPVRTGDSVVYSFSATD
                     VAGFSTQSAAWTVQVAYAQFDFVPTSIVAGLPANTATNLTLQIENSGDLPLVLDIVPV
                     FAGFADDMESGTNDWTRPDGNQDWHISAQDAHSPTRAWYCGREATRTYRELTHAALVS
                     PPIQLAAESRLSFWHHARFENDVDDILDGKHYWDSGVMEISTNSGASWLPLEPDGGYP
                     GRITENPMSPFAAETPCFASTLGWEPVTASLADYAGQMVQIRFRFGADRYVNEEGWRL
                     DDVTVSPNSAATNWFGLSTTNLTLTNGYSALVQLQLDTTPLPPMGSAAVALYLHHNDP
                     EQPSPRVLLVQLQNLTRRVDITTSGPGQAVPTGTFLLAPAEPLEVELTAEPNHFIADI
                     LTNGVPVPIPNVFTTQTLSWSSLPGNLALHAIFMPLLPDNAVPPEWLAGYDLTTRHWM
                     AEASLDHDGDGLLTWQEYELDSDPLDPESAPLQVNLLAPDEPTNAWRISWHAFTNANA
                     TYTVLGSTNLTMGFHALTNILAAPPVMTSPPLPPDHRFFGLQKTLP"
     gene            complement(8793..9221)
                     /locus_tag="PKI64_08205"
     CDS             complement(8793..9221)
                     /locus_tag="PKI64_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOE01027.1"
                     /translation="MNLLLGIGNPRHGDDGLGPLFARAFRHPEWRTINAATAPENFTG
                     LIRRIRPELLVMLDAAEMGLPPGTLRRLQPAEIGARDFGTHAPSIGQLATYLAECAGQ
                     VVILGIQPATTQPGAHLSPPVRVTLRDLARQLAAGRLPPE"
     gene            complement(9275..9463)
                     /locus_tag="PKI64_08210"
     CDS             complement(9275..9463)
                     /locus_tag="PKI64_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOE01028.1"
                     /translation="MKATVDAGTCTGCTLCCDLAPDVFVMGDDGVAHPTADVVPAASE
                     GAAQEAAASCPVTAITLS"
     gene            complement(9513..10088)
                     /locus_tag="PKI64_08215"
     CDS             complement(9513..10088)
                     /locus_tag="PKI64_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOE01029.1"
                     /translation="MKSIKGTRTEKNLLASFAGESQARNRYTYFASVARKEGLIQISK
                     IFEETADQEKEHAKRFFSFLEGGMVEITAAYPAGVVSSTLDNLKAAAAGENEEWTKLY
                     PEFARIAREEGFEQIATLWEKVSNAEKQHEKRYNDLWQNLSAGKVFKRDGVVVWRCIN
                     CGYLHEAQEAPKLCPACLHPQSFFEILGENW"
     gene            complement(10132..>10775)
                     /locus_tag="PKI64_08220"
     CDS             complement(10132..>10775)
                     /locus_tag="PKI64_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOE01030.1"
                     /translation="KSNLACNFGCPYPEGYRKALRLMRLAAKFNRPIVCFIDTPGAFP
                     GIEGEERHVAEAIAVNLREMFRLAVPIIAVVIGEGGSGGALGIGVANRILILEHAYYS
                     VISPEGCAAILWKNRSHADTAAAALKLTAPDLVELGIADDIIPEPQGGAHADPAAAAE
                     LVRAALEKHLAQLLKLSPAQLLAQRYDKFRAMGVFKEGPLPALTPPPIPADNA"
BASE COUNT         2111 a         3004 c         3558 g         2102 t
ORIGIN      
        1 gtttagggcg ttttttgacc gaaatatggc ctaaaatcct cgaaaaacac cccgatgccc
       61 gtttgcgcgt cgccgggcac ggccttccgg cggaacaacg cgaaaaatgg cgcaaaatcc
      121 gtcaagttga actcctgggc ttcgtttccg atttggttgc cgaatacaac caatgccgcg
      181 ccgtcattgt cccgatttac acgggctccg gaaccaacat caaagtgctt gaggccatgc
      241 aaatgggccg cccgtgcgtc atcaccaatt ttgccgccaa aggctttgag accctcctcc
      301 aagacgggaa aaacatccta atcgcccaaa acgacgccga atttgcccat aaactccaaa
      361 aactcctcga aaaccccgat ttttccgccc aaatcgccca ttccgcccaa tccgacatcc
      421 aaacacacct caacgccatc tcctactccg ccattttgtc gaagtttctc taatcaatgg
      481 cgtgttttta aagtaacaga aggggtaacc cttattataa tcttttccgg ttttgtagcc
      541 atcacgctga agcctaacct catacgatgc accaaatcgt gcttggagat gggcaggatc
      601 ctgtagtcag ccaatggagg gataagatcc atcctgtccc gcgtccttgg aacgattccc
      661 gcgtacgttg caccactatt ttctagttta atcactcagc aataccgtgt gatatgagca
      721 tagagcactg cctcggcttt gtctaagttc aggaagagcg ggggggccgg catggcggca
      781 gcggtgtaac ccaacactgg gcaaacaacc tcagcacttc gattccacac gcttcgtgtt
      841 tcagtccatt gcgtgacaat aatggctctt tcctcctttg gacatatgca tgaccatatt
      901 ttcggtgtca tttcatttga ggcaacctgg caaacggggc cccacgcaga gaaatatttc
      961 ggtgtcatga attgtgaggc aacgtgccac agccgccggc gcctttttgc gcttggccgg
     1021 ccgggcggcc ggcggtaagc tgttgttgtg ggcgaacgcc ccgcgggagg atctgcacat
     1081 gaaagaaagc gcagaaaaaa aaggcgggaa actgaaatat cgcaaggagc tggccaagct
     1141 gcaggtggaa ctggtgaaac tgcaggaatg gatcaagcag aaggggctga aggtggtggt
     1201 gattttcgaa ggacgcgatg ccgcaggcaa gggcggcgtc atcaagcgca tcaccgagtg
     1261 cctgaatccc cgtatctgcc gggtggtggc gcttggggtg cccaccgata aggagaaaac
     1321 gcagtggtat ttccagcgat atgtggcgca actgcccgcc gccggcgaga tggtgctgtt
     1381 cgaccgcagt tggtacaacc gcgctggcgt ggagcgcgtg atgggatttt gcaccgacgc
     1441 ggagtatgaa gaattcctgc gctcgtgccc ggactttgag cgaatgttgg tgcgttccgg
     1501 gatcatcctc atcaaatact ggttttccgt atcggacgaa gaacaggaac ggcgttttca
     1561 ggcgcggatt gatagcccca ccaagcggtg gaaactcagc cccatggatt tggagtcccg
     1621 cgcgcgctgg gtggaatatt ccaaggccaa ggacaagatg tttgaagtgt gcgacattgc
     1681 ccaagctccg tggaatgtgg tgcatgccga ctgcaaaaaa cgggcccgct tgaattgcat
     1741 tcatcacttg ttgagccaaa tcccctacaa ggatttgacg cccaagccga tgaagttgcc
     1801 gccccggcaa aaacgcaaag ggtacgtgcg cccgccgttg tccgaccagc atttcgtgcc
     1861 ggaagtgtat tgattccggc acactccgtg gcgcggctgc gcggccttgt gcaaaacgcg
     1921 gcctaaaatg gcaaagattc ctgttgcatg gggcaggcag gtaggctact gttcggcgac
     1981 ttttgcttcg gcagaggccg acgaccccat cgtctagagg tctaggacac caggttttca
     2041 tcctggtaac acgggttcga ttcccgttgg ggtcgccatt tttgaagcga gctggaaagg
     2101 caaggagaga gggtatgtcc cggaacgtga gaaacaatga tacgacgcgc ccgcgcaaga
     2161 gcggcgcggc gaagaatcgc cgtttgttgg agcaccgccg gcggctggtg gcgttgggtg
     2221 tggatgaaca gaaggtgctg cagatgccgc acgaccgtat tcgcctgttg attgccaagc
     2281 cgaacttgat caagcgctac gtgcagtagc ccttggcgtg cttgacgccg tccgcttccc
     2341 aacggaagcc gggcggcttt tttgttgccc gttggtggcg cttactcgcc ggggaacggc
     2401 cgagcttgga cgcggaaaaa ccgtgtggag gccggcggca aaatatcgtc cgaccagaac
     2461 atatccacaa tgggccattg ggaggtgggg ggcgcgggtt gccatgagtc aggttgcagc
     2521 gtcgcactat attccactgt gaacacgttg gtttcgttga tgttggtgct ccagattcca
     2581 gaccagtgca aaacgtggga ttcaaactcg tggatgcgcc cctcgcggcg cagcaggaag
     2641 gcgcgaggct cgccgttgaa aaggccgttg ccggcaattt cgttggcatc attgatggcc
     2701 gtggcgttgg tcagcaccca gccggtgtcg ggcgggatca atgaattgag gttcagcatg
     2761 gtttcattac gccaaagaaa agcttgattg gtgccggcgg gggtgtcgga tgtgccgacg
     2821 atccagccgt gattattcag cgcattggcc gaggagttgt tggtgggccc acccaaggtg
     2881 ccgaggctgc gcatcagcaa attggttggg tcggtgttgc tgtaggaagg taatttccaa
     2941 atgccattgg aaggggtgat caaaaaagcg tggcggggat aatatccgtt gctaatggcg
     3001 ctccacccga ccacgaggcc gtggtcatta atggcattgg ctgcactcac ttcgccaacc
     3061 gaatccttgc tgcccaaagt gcccagcaac ttcatttcgc cgttgtcgtg gagcccgttg
     3121 gtgttgtcgt catgccaaac ataggcccac cagcgcgggt tcggagagta aacgaacgtg
     3181 atgccacaga cttggccgag gttgttgatg ccatacgcgt cgcaagaatt gccgggcgca
     3241 aaggcgggga tttgcgtgta ttggttcgtc tgccaataga aggatgaatt atatgatgaa
     3301 ttattggtgg ggggcgcggt gccggagatg gcaccctgat cattgatggc tctgcccatg
     3361 ctgttgatgc tgccccaggt gccgatattg atcatgctgt tgctgacgta taaaaaggcg
     3421 cgctggttgg ccgcagaaat gtgggcatgg ccggtaattt ttccgccatt gttgatggcc
     3481 atggccacgc tggacgtgcc gccgggcaaa aagccaaggc ccgtcatgcg cccgttttgc
     3541 caaacaaaag cctgatggcg accatcggca tccaatgacc atccgaccac ttggccgcgg
     3601 ttgttaaggc cattggcttc ggcgtggttg ccgcccaacg tgcccaagtc agtcaccgtg
     3661 tattccgcgg cggcattggc atgaaccgcc aacagcaggc cgacaacgaa aaaccaccac
     3721 atggcgcgcg aggtgttcat cggggaacag tgtgtcactc ccgggcggcg gggtcaattc
     3781 aaggcagagt tttttgcaga ccgaagaagc ggtgatccgg gggcaggggc ggagaagtca
     3841 tgactggcgg ggcggcaagg atattggtca gggcgtggaa gcccatcgtg agattggtgg
     3901 aacccagcac cgtgtaggta gcgttggcgt tggtgaaggc gtgccatgaa atgcgccacg
     3961 cattggtcgg ctcatcgggc gcgagcagat tcacttgcaa cggcgcggat tcggggtcga
     4021 gcgggtcgct atccagttca tactcttgcc acgtcagcag gccgtcgcca tcgtgatcca
     4081 aactggcttc ggccatccag tgccgcgtgg tcaggtcata gccggccagc cattccggcg
     4141 gcacggcatt gtcgggcagc aggggcatga agatcgcatg cagggccaag ttaccgggca
     4201 ggctggacca cgacagggtt tgcgtggtga acacattggg aataggcacc ggcacgccat
     4261 tggtcaggat atcggcgatg aagtgatttg gctccgcggt gagttccact tccagcggct
     4321 cggcgggagc gaggaggaag gtgccagtgg gtacggcctg cccggggccg gaggtggtaa
     4381 tgtccacgcg gcgcgtcaga ttttgcagtt gaaccaacag aacccgcggg gagggttgtt
     4441 cgggatcgtt gtgatgcaga taaagggcca cggcggcgga gcccatgggt ggcagcgggg
     4501 tggtatccaa ctgcaactgc actagagcgg aatatccgtt ggtcagcgtc aagttggtgg
     4561 tggacaatcc gaaccaattg gtggctgcgc tattgggcga aacggtcacg tcatccaggc
     4621 gccagccttc ttcgttgacg tagcggtccg ccccgaagcg gaagcggatc tgcaccatct
     4681 ggccagcata atcggccagc gatgccgtca ccggctccca accgagggtg ctcgcaaaac
     4741 aaggtgtttc ggcggcaaat ggcgacatcg gattttccgt gatccggccg ggataaccac
     4801 catccggttc cagtggcagc cagcttgccc ccgaattggt ggaaatctcc atgacgccgg
     4861 aatcccagta atgcttgccg tcgaggatgt catccacgtc gttttcaaaa cgggcatgat
     4921 gccagaaaga gagccgcgat tcagccgcca gttgaatggg cggcgaaacc agagccgcgt
     4981 gggtcagttc gcggtaggtg cgcgttgctt cgcgcccgca ataccaggcg cgggtggggg
     5041 agtgcgcgtc ttgggcgctg atatgccaat cttggttgcc atccggccgc gtccagtcgt
     5101 tggtgccgga ttccatgtca tcggcaaacc ccgcaaaaac gggcacaatg tccaatacta
     5161 gtggcaaatc accggaattt tctatttgca gggtgagatt ggtggcggta ttcgcgggta
     5221 ggcccgccac aatgctggtc ggcacgaagt cgaattgcgc ataagctact tgcaccgtcc
     5281 atgcggcgga ctgtgtggaa aagccggcga catcggtggc ggagaaggaa tagactacgc
     5341 tgtcgccggt gcgcacgggg gaagggatcg cgccggtaaa cacatccccc gcgctgttgg
     5401 tcatgggggc tgagcggggc aggccaccgt tgcgctgcca gtgcagggtg gcggtggcca
     5461 cgcggtagtt gtccgtgata atggcgctca gcaaccacgg ggcgggactg gccacgggcg
     5521 aggccagcgg ctcgtgcgtg atgccgggcg caacgggcac ggatgccgaa tcgagcggat
     5581 cggtgtggtc ggcggcttcc acctcgtttt cgtggccgtc gccatcgggg tcggcgaagc
     5641 gggattggcc aagcgcgcca aagtagcgca gctcaaacca atccggcagg ccgttggcat
     5701 cgctatcctg ttccgttggg agataggtgg cagtcgcggc gcgcggctcg gtcatgggga
     5761 tgccgtccac ttggtgctgc gatggttcgc cgttggtggg ccaacgcgcg ccatccacca
     5821 gccagccggc aaacgtatag ggtgtctggc cgaccagcgc actttccggc gccggcagcg
     5881 aggaggctgc ggagcccaat tcgtgccaga catttgtgtt gaggatgccg tgcggctcgg
     5941 aaatgtgcat caacaaggct tgctcctgcc attgccacac gagggaggaa tcggcggtga
     6001 tggtgaactc gcacgtattg gccacaccgg cggccggcac gaagccgacg ccctgccagc
     6061 cgacacatgc cgtgcgcatt ccgttggtgc cggtggcgga aaaagccgcg tcagcttgca
     6121 ccacgatgcc ggaggcgaag gtgtgcgtcc cgtagccggg gttcaccgca aaatattcgc
     6181 cgggcgcgcc ggtgatggtc agcgtcagcg gttgcgtgac ggcgaattga tgtagggccg
     6241 ccggcgcttc gggcgggtgg gtggtcagga ggtcagccgg cccggcagaa agataatagt
     6301 acacccaagt atccgtcggt tgggcgggaa tggccgcgct gaacatctga ttgctgccgg
     6361 gcgtcattgt gaccacgttg aaatcgtcgg tgctgcccgt ggtgttccag tggagttgca
     6421 cggtgtcggg gtccaattgg cccggcgagg tgacgcgcgc cgtgaccgta tattccacat
     6481 tggtttgcca cgtgttgtcc agcggcgcgt gttcgatggc cgcgggcaga tcaatggcgc
     6541 cgcgcatata aatcgcgtag ggctgggggc cttcgggaac attatgagca gaaaccacca
     6601 acgtccagcg tccggtgact ggcgcatcca ggtcaatgcc ttccacgtta ttcaggcgat
     6661 ccggctccgc caagccgttg ggaaaatatg aggcgccgtc ggggtcatgg agcgcgaggt
     6721 ccaaatcatt gacgagattc acggcggccg ccagcgcgga gggataatcg ctataggcca
     6781 acgtggcggt cagcggcgcg gactcacgca cgatgaactc cagcgtgttg ctgccgccgg
     6841 tggtgagcgg cgccggggaa tccagcaagg ccacttgtgt tgcgccggta gggaacaggg
     6901 tgccggccaa attgatttgg ccgaatcctt caaccgaatt agggcgcggg ggggcgggga
     6961 tttcgcggaa ctcattggtg ccgtattggc cgggcgagag tgagcgcgcg ccgccgacca
     7021 gcgccgcctt gagcaaggcc gcggaggggt tttccagccc gcgtgtttcc acgcaaaact
     7081 gccggagaag cgtggcgcat ccggtggcga atggcgcggc catgctggtg ccggtcatga
     7141 agcaataatt ggtgttgccg gacagcacgc cgcgccccgt gctactggag gctcgcgagc
     7201 gcgcggacac cacatcgcta cccggcgcga ccacatcggg cttggtacgg ccatccatcg
     7261 cggggccgcg tgaagacagc gcagaaattc cctgtgggcc gccgccgggg ctggaggata
     7321 tcaggtcgct gaggatgggc tctgtgcgaa agcgcgatgg ataattcatg ccgtaatttt
     7381 gggcggtttg gccgccgctg ccggccgggc gtccactttc gctggcgccc accgccaaca
     7441 catttttggc ggaggccggg gcatccaacg attgctcatc aactacgcca tcgcgattgt
     7501 catctgtgcc ttcattgccg gcggcaaagg ccacaaacaa gtcggggtga tcccacacaa
     7561 actcatcggt ggtcatagaa tcgctgttgt attcgccttc cgccgcgccg ccccagctat
     7621 cggaatgcag ccgcgcgccg gcatcatagg cgggctggta caactcgttg agatcatccg
     7681 gcagattgag gcgcgaggaa gcgtccatca cggattgcag caccagttgc gcgctgggtg
     7741 ccacgccgcg aaactggttg gtggaagccg cgccggtgcc gagcaaggag ccaatcacgt
     7801 gggtgccgtg gtagtacttg tcgctccagt cattgccgcg gccagtatcg aagacttgca
     7861 gcacttggcc gacaaaatcc ggatgcagcg aggcgagatt tccggaatcg agcccggtat
     7921 cagccacggc tacgatttgg ccagcgccgt cgaggccgtg gtcgtcatgg gcggcatcca
     7981 cattcaggcg gttggcggcg cgggcgacat cattcaacag gcgcggggtg gggcggtgct
     8041 cgatccattg cacatcggcg cgggcggcca gagccgcgat ctcacccgcc ggcacgaccg
     8101 cgcggaccaa gccccagcgt ttgccgggtg tggcgctgac ttcgccggct tgcgcggcgc
     8161 gcaagacggc cgcaaccgcg aacgcatcat cgggcgcaaa ggtctggata gtgaccggca
     8221 gcgcgatggc gggcgttttt ttcaggaggg cggcgagcag gggttgcagt ttgtaggccg
     8281 gccggtattc cccatgccaa gccacgtgcg gcatgtgggc cagccggcgg atggtggccg
     8341 cgggcgcttc gaccaacaac gcgtgatgtg gcagataggc gcgcacggtg gcgccggcat
     8401 cctgcaaggc ttgccgccat tccggttgca gcgggccatc gaacaacacc agccacggat
     8461 aaccacgcgt ggaaggtgta tggtgaggct gctcatccag cgggccggga aagaggtcac
     8521 ccgcccgcag ccggatcact gggcggtcgc ccagcggggc aggggggggc gtgcgggtct
     8581 gcgcgcgcgc ttgttggatg gcggcatggg cttgttgatt ggcttcgacc gccgttggcc
     8641 ccggcgggga ccacgccaga ttcaggcgcg ggtggctcca ccagagcgtg gccaaaaaca
     8701 tcagccccgc agcgatcagc aattgccggc tggataacgc gcgcttcatg tcaggtggtc
     8761 atccggcaat ttaccatacc tgcgggataa tctcactccg gcggcaggcg tcctgcagcc
     8821 aactgacggg ccaaatcgcg tagcgtgaca cgcaccggcg gcgagaggtg cgcgccgggt
     8881 tgcgtggtgg ccggttggat gcccagaatc accacctgcc ccgcgcattc agcaagatag
     8941 gtggccaatt gaccgatgga tggtgcgtgg gtgccgaaat cgcgtgcgcc gatctccgcc
     9001 ggctgcaacc gccgcaatgt gcccggtggg aggcccatct cggccgcatc cagcatgacc
     9061 agcaattccg ggcgaatgcg ccggatcagg ccggtgaagt tttccggcgc cgtggccgca
     9121 ttgatggtgc gccactccgg atgccggaag gcgcgggcaa aaagcggccc caagccatca
     9181 tcgccatggc gtggattgcc gatgcccagc aggagattca tgataaaaaa aggccgccgc
     9241 tggcggcggc ggccgtgctc ggcagagccg ggcgttagga cagcgtaatg gccgtcaccg
     9301 ggcagctcgc cgcggcttcc tgggccgcgc cttcggaggc cgccgggacg acatcggccg
     9361 tcgggtgggc cacgccgtca tcgcccatca caaacacatc gggagccaga tcgcagcaaa
     9421 gggtgcaacc ggtgcaggtt cccgcatcaa cagtggcttt catttcggta ttcctttcgg
     9481 gttaggggtg ggggtccaac atcagcggaa cattaccagt tctcgccaag gatttcaaag
     9541 aaggattgcg ggtgcaggca ggccgggcac agcttggggg cttcctgcgc ttcgtgcaga
     9601 tagccgcaat tgatgcagcg ccagaccacg acgccgtcgc gcttgaacac cttgccggcg
     9661 gagagattct gccagaggtc gttatagcgc ttttcgtgct gtttttcggc gttgctgact
     9721 ttttcccaga gggtggcgat ttgctcgaag ccctcttcgc gcgcgatgcg ggcaaattcg
     9781 ggatagagct tggtccattc ctcgttttcg ccggcggcgg ccgccttcag gttgtccagc
     9841 gtggaactga ccacgcccgc ggggtaggcc gcggtgattt ccaccatgcc gccttcaaga
     9901 aagctgaaga agcgcttggc gtgctctttt tcctgatccg cagtttcctc aaaaattttg
     9961 gaaatctgga tcaagccttc cttgcgcgcc acggaggcaa aataggtgta gcggttgcgg
    10021 gcttgggatt cgccggcaaa cgaggccagc aggttttttt cggtacgggt tcctttgatg
    10081 cttttcatgc ggtctcctga ttagcgggcg gggcccgcgt gttgtcaaat cctaggcgtt
    10141 atctgctgga atgggcggcg gtgtcaaggc cggaagcggg ccttccttga acacgcccat
    10201 ggcgcggaac ttgtcgtagc gctgggccag aagttgcgcg ggggataatt tgagcaattg
    10261 ggccagatgt ttttccagcg ccgcgcggac caactccgcg gcggcggcgg ggtcggcgtg
    10321 ggcgccgccc tgcggctcgg ggatgatgtc atcggcaatg ccgagttcca caaggtcggg
    10381 cgcggtgagc ttgagcgcgg cggcagcggt atccgcgtgc gagcggtttt tccacaaaat
    10441 ggcggcgcag ccttcggggg aaatcacgga ataataggca tgttccagaa tcaggatgcg
    10501 attggccacg ccgatgccca gtgcgccgcc cgagccgccc tcgccaatga ccacggcgat
    10561 gatcggcacg gccagccgga acatttcgcg cagattgaca gcgatggctt cggccacgtg
    10621 gcgttcttcg ccctcgatgc cgggaaaagc gccgggggtg tcaatgaagc acacgatggg
    10681 ccggttgaat ttggcggcga ggcgcatcag gcgcagcgct ttgcggtagc cttcgggata
    10741 ggggcagccg aagttgcagg ccagattgct cttgg
//
LOCUS       DAOPNP010000087        10553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_7161_length_10553_cov_6.989141, whole genome shotgun sequence.
ACCESSION   DAOPNP010000087 DAOPNP010000000
VERSION     DAOPNP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7161_length_10553_cov_6.989141"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..7862)
                     /locus_tag="PKI64_08225"
     CDS             complement(219..7862)
                     /locus_tag="PKI64_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOE01031.1"
                     /translation="MNNKHLFSLVWGMGGMALLLAAGLAWADPEPEPARRAKRLATPQ
                     EENAVAGQNAWDETGPDIVTDFVLPEAQDETPLVAEARRLARSQARVTGASPDEVEQA
                     AWLIPDDLDERKSEHQLALEAHAGEPPGDTSGEEAPLPSRRPTARGILGAPQIAAGKR
                     VLGWHPYWATSSDLANYQFSNLTHLAFFSYEVNPANGNATDMHGWNTTPMVSWAHSNG
                     VKAVLTVTLFGSANNQQFLSNPAATTNLIRNLLVAMTNRAGGAGDGVCIDFETIGSWS
                     GSGAAMTSFMTNLIKKFHDANPNYEVSIALPAVDWSANFNVSAYNTAGMDYAIIMGYD
                     YYYSGSSTPGPVAPLFSSAQWVGANSWCSDNYSITNYLGKGFPASKLMLGVPYYGRRW
                     AAASTNLGAASLGSSYSVALTYGAAKSEANKYGRRWNDNASVPYYVYTSNSTAYQCFY
                     DDTLSLGMKYDLANTKGLGGIGIWNLTQGTGQTELWTLIADKFGADAGPPDDPGSGDP
                     GGSASAPWVSRTSAHTANFYGVGSRAGLHVASGAGGAIYTSTNGINWTARTSGKSGLL
                     MNVNGDGPLWVIVGEGGAILTSTNGLNWVSRTSPTNALFRGVAYGNGIYVACGDGGAI
                     IRSTNGITWSSVTSGVSDSLQGVGYGAEFISTGDTNNPTAPSPLFVVSGANGTILTST
                     NGVAWTKRTSGTTVYLSDVTYGNGYYVAVGNTRTLRSADGLTWSFTTNSVYLYRVAYC
                     SGAFRAAGKNGVIWTSADGLTWSAETSGTTNDLRGMSYTADQFVSVGFNGTILTKGTM
                     AGQESGEPGDEGGSGETGGGDLPVTTPDETGLPQVAAKQPTGPLSGVVVFTSAGHGFG
                     ANEALTAWIPERPLLNAVNEDMGNIDQLNYFAESAWKAGATVVPFRPVGYQTNEVVLD
                     NMDTNLTARGQVTFGGTWFNSTNSVYYGKSGDAVSYRYAAVNSSETTAWATYRPNLPA
                     AGDYPVYTWVLQSGNRTNQLYRIYHSGGVTDVRVDHSAVGCGWVWLGTYHFAKGTNGC
                     VNISNYVPGGQEGFNVIADAIRFGNGMGTTARGSAGVSGFESELESSRFWVIKSIGQG
                     IPSTLYDMAGYDDRNDNIGQPARMADLMCRTNDRPRWRRVYVGFHSNAGGGAGARGSI
                     GLYDTRIQANYPSFYQAQTNLAVALARQCNEDMRAGVTDGVIPSWSSRTSYYYGSTYG
                     ELYNAFNEYVFKKMDTTINEVAFHDNADDCTVLKTPAGREWLARSSTRGLIKHLSGYY
                     ASGIINTNAPDRPIKLKAANSGAGAVTVSWAMPTRTAASGGVPQGFIIYTSTDGRGFG
                     NPITVSGGATTSKTITNLNAGATIFFRVCATNAGGESLNSQVAGVRVTAGGSKAGVLV
                     VAGFDRNDSSLAPTRYFANGLNGQVTLVRPKMINSGDYVKEHGLALAAAGQTFDFMDH
                     TLVTGAAVLTNYAKVVWMLGEESTVDETFSSGEVTAVTGYLNAGGRLFVSGAEVGYDL
                     GRAAISTPAELAFLTNMLRTTYIADSGNTGQVTGTASGFLNGVTIHFNYTNLLTDIYA
                     ANYPDVLGAGSGAAKAAVYGASAGGANGAIIQYSNATYRTIVMGFPFETITNEATRAT
                     VMTKAMEFLSDATVPGAVKVTLAPAGAAGTGRWIIANTTNTSGSTRTGLAPGPYQVTF
                     GPVAGYLAPAATSIVVTAGTTNAFTATYTAASGALTVNLQPASAQTDGRWRINGGTWH
                     TSGTTVSDLTNGTYAITYKDITSHTTPATNNVTISGSPVTITGTYVAQVGNLTVTLTP
                     AAAVAKGAQWSVDGGTTWYNSGATATGLLAGRQYLTFKTVDGYTVPSTTYTTVTAGET
                     VSRSYAYSAQPGSIKVNLSPAGAVATGAQWSLDGGTNWNESGATVLDLGVKTYTIVFN
                     TVAGYTAPATISHALAAGENATLSAAYDEIQLVGADCIRSQGFDDLDAHPWSWSTIYL
                     NDSGVTTAENHGAVVVVSTNKVLAGANGLRLSGSTNGAANPTILFSNVDISGYTNVTL
                     TIPFAANGPDNGDDLHVAVSYNGGSTWLPSVWGTQIADGNNNVKLEYNTFVDTNRQPV
                     GTPYVLAVDDSRTQIMVRVTFYDAISANTSDHYYLDEIQLKGQVGTPAAPSSAVKVTI
                     TPEDAIYAGAQWRVDAGAWRNSGTVVTGLTAGSHTVSFKSIEGWLEPADVVTTTTNSS
                     TNALSAAYLEKPAGPVTIMQDDFEDGDLVGWTQDSAGNWANSTNAAITGNRSLKHNLS
                     NVAVVNYVYAQPSYNVALDTTTWRFKLKNGNWDPSADNRFHVYLMASASDLAGTTVNG
                     YAVGVNFTGTDDLIKLWRVTGGGVGSTVLTSVVDWNAKMTVAIEVTRTAAGLWELKTS
                     TDGLFSGMTSAGTATDTTYTDTSYFGLYYKCTSTRAGEVWLDDVLIHQGELSSATDSD
                     GDGMPDSWEQAHFGSATGADPNADADGDGMSNLAEYIAGTDPRSGNSVLELTALNVVK
                     SSTTNLVIQWPSVTGRIYSIWLAPSATNAYTLHRDNLSGSGGTLSITNAGPAGDNSHF
                     YGIKVRLAP"
     gene            8082..>10553
                     /locus_tag="PKI64_08230"
     CDS             8082..>10553
                     /locus_tag="PKI64_08230"
                     /inference="COORDINATES: protein motif:HMM:NF023351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HOE01032.1"
                     /translation="MSTLWTRRILALVVIMGICSAATTFAAKTKPKKEAPPPEVSETE
                     APAFPPAELTVGFQARDSETEGIGDLLVPVWNPGGAGLLFLNPRTAITDHDGEEANIG
                     VGYRQLLPKWNVILGANAFYDYRDTGAYNYHQWGFGLELLSQWLDARFNYYDPEDKTY
                     VAATTTETSTRQSVRATTRWEDPYATGHEVRQNLLTIRQLTTTTTTRTFELFEQPYGG
                     HDWEIGLRLPINCNGFEARLFGGYYDFDRDFGAAARGWKTRAEIRLRSSLFLDAGLYE
                     NDKLTGSDWYLGARLTTPLDLAQLASGRNPFATAKARWSGEPADLASRLTEMVMRDPQ
                     IRLEHSKFIENPQLRTETATTTKTATRKRHVLLPDVQFVNGDAPAGGDGTGERPFNQI
                     QQGADSVFGDRNVYVYNASGPYMENVVLQAGTTLCGSGVQIPGYGGKTFGSGIAPVVD
                     GNNLGPSITVANQTTVKGFSIRNTDRGLPPIIFDTPAFYNVNPARMGIFGNGATDVTI
                     TRNLLANNETAAHFSRIGSFRLNFSDNTVEDNSLVGLEICTAGAGLPSSDTCEATVAR
                     NSIQNNGSIGLYSTMWNYQSSLLDIADSDISRNTVGVSSSQAESGDAILIVRNLHADH
                     NAIDGLLAEQILNINNLIAVAGSSFDHNTGYGVNSDQRAEENSLAFFGMPEGLDQLVN
                     AVGAFGGPVPAFLAPLLASAGGVSASHNGRGGIGCGIYAQNDAALGAFFDVTANGNEF
                     SGLVAEIVSDGNLAVGLAGGSGTLNNLLNLGSDAVAGMLGIDLPVLALGNGEMQVNDN
                     SSFGMLMAVRGDTALAAVAGVTANGN"
BASE COUNT         2039 a         3308 c         3013 g         2193 t
ORIGIN      
        1 ctacaatccg atcgaatcaa gcgatggctg ttcttgtagg gcgagaatgg ttgaggaacg
       61 aaaccttccc gccgggcacg gctggaaggt ctgcgaccat tccagcccga caatccgctc
      121 gaatcaggcc atggttgttc ttgttgggcg ggaatggttg agggacgata tcttcccgcc
      181 gcctcttccc ttcaacgtgt gtcgcggtgc gccttgcgtc acggcgcaag acgaaccttg
      241 atcccgtaga aatgcgagtt gtcgcccgcg ggaccggcgt tggtaatgct gagggtcccg
      301 ccgctgccgg agagattgtc ccgatgcagc gtataggcat tggtggcgga gggtgccagc
      361 catatggaat aaatccgccc ggtcacgctc ggccattgaa tcaccaggtt agtggtcgaa
      421 ctcttgacga cattcaacgc tgtcagttcc aacacagagt tgccgctgcg cggatcagtc
      481 ccggcaatgt attcggctag attgctcatt ccgtcgccgt ccgcatcggc attcgggtcg
      541 gccccagtcg cgctaccgaa atgcgcctgc tcccacgagt ctggcatccc atcaccatcg
      601 ctgtccgtgg cgctgctcaa ttcgccttga tgaatcagca catcatccaa ccacacctcc
      661 cctgcccggg tgctggtaca cttgtaatat aaaccgaaat acgaagtgtc agtgtacgtg
      721 gtatcggtgg cggtgcccgc tgaagtcatg ccgctgaaga gcccatccgt gctggtcttc
      781 agttcccaca atcctgccgc cgtgcgcgtg acttcaatgg ccactgtcat cttggcattc
      841 cagtccacga cggacgtcag cacagtcgag ccgaccccgc cgccggtcac ccgccagagt
      901 ttgatcagat catcagtgcc cgtgaagttg acccccaccg cataaccgtt gacagtcgtg
      961 cccgcgagat ccgatgcgga agccataaga tacacatgga aacgattgtc ggcggaagga
     1021 tcccaattgc catttttcag tttgaaccgc cacgtggtcg tatccaaggc cacattgtag
     1081 gacggctggg cgtagacata attgacaaca gccacgttac tcaagttgtg cttgagcgaa
     1141 cggttgcccg tgatcgcggc gttcgtgctg ttggcccaat tgccggcact gtcctgcgtc
     1201 cagcccacca aatcgccatc ctcgaagtca tcctgcatga tggtgaccgg gccagccggc
     1261 ttttccagat atgcggcgct caatgcgttg gttgagctgt tggtggttgt ggtgaccacg
     1321 tccgccggct ccagccagcc ctcgatggac ttgaacgaca ccgtatggct accggccgtc
     1381 agccccgtga ccactgtgcc actgttgcgc cacgcgccgg catccactcg ccattgagca
     1441 ccggcataga tggcgtcttc cggggtaatc gtcaccttca ccgcgctgct cggcgcggcc
     1501 ggcgtgccaa cctgcccctt caattggatt tcatccagat aataatgatc ggaggtgtta
     1561 gcagatatgg cgtcatagaa ggttacccgc accatgattt gcgtgcggga atcgtccacc
     1621 gccagcacgt agggagttcc aaccggctgc cggttggtgt ctacaaaggt gttgtactcc
     1681 aatttgacgt tgttgttgcc atcggcgatc tgggtgcccc aaactgaggg aagccacgtg
     1741 ctgccgccgt tatagctgac cgccacgtga aggtcatcgc cattgtccgg cccgttggcg
     1801 gcaaacggaa tcgtcagggt gacattggta tagccgctga tgtccacatt gctaaaaaga
     1861 atcgtgggat tcgccgcgcc gttggttgaa ccgctaaggc gcagcccgtt ggcgccggca
     1921 agcactttgt tggtgctgac caccaccacc gcaccatgat tttccgccgt tgtgaccccg
     1981 ctgtcattca aatagatggt gctccatgac caagggtgtg catccagatc atcaaagccc
     2041 tgcgagcgga tacaatccgc gcccaccaat tggatttcat cgtaggccgc gctcaaggtg
     2101 gcattttcgc ccgcggccaa tgcatggctg atcgtggccg gtgcggtgta accagccacg
     2161 gtgttgaaca caatcgtgta ggttttgaca cccaaatcca gcaccgtggc gccgctttca
     2221 ttccaattag tgccgccgtc gagactccac tgcgcgccgg tcgctaccgc gccggcgggc
     2281 gataagttta ccttgatgga acctggttgc gctgagtagg catagctgcg gctgaccgtc
     2341 tcgccggccg tcaccgttgt gtaggtggta gagggcacgg tgtagccatc cacggtcttg
     2401 aaggtcagat actgccgacc ggccaacaat ccggttgctg tcgcgccgct gttgtaccat
     2461 gtggtgccgc catccacgct ccattgtgca cccttcgcca ctgctgctgc cggcgtgaga
     2521 gtcacggtca aattccccac ctgcgccaca taggtgccag tgatcgtcac cgggctgccg
     2581 gatatggtca cattgttcgt cgccggcgtg gtgtgactgg taatgtcctt atacgttatg
     2641 gcataggtcc cgttggtcag atccgacacc gtcgtgccgc tggtgtgcca ggtgccaccg
     2701 ttgattcgcc accggccatc agtctgggcc gatgccggct gcagattcac agtcagcgcc
     2761 ccggaggccg ccgtgtatgt cgcggtgaat gcattggtcg tgcccgccgt caccacaatg
     2821 gatgttgccg ctggggccag ataaccggcc accgggccaa aagtgacttg gtacgggccc
     2881 ggtgccagcc cggtgcgcgt gctgccgctg gtgttggtgg tattcgcgat gatccagcgg
     2941 cccgtgccgg ctgcgccggc cggcgccagt gtcactttga ctgcgccggg gacagtggca
     3001 tcactcaaaa attccatggc cttggtcatt acggtggcgc gggtggcctc gttggtgatg
     3061 gtttcaaacg ggaagcccat cacaatggtt cgatatgtcg cgttgctgta ttgaatgatc
     3121 gccccattcg cgccgccagc gctggcgcca tacaccgcgg ctttggccgc gccgctgccc
     3181 gcacccagca catcgggata gttcgccgcg taaatatccg ttagcaggtt ggtgtaattg
     3241 aagtgaatgg tcaccccgtt aaggaaacca ctggcggtac cggtcacttg gccggtattg
     3301 ccgctgtcgg caatatacgt cgtgcgcagc atgttggtca ggaaggccag ttccgccggc
     3361 gtggatattg cggcgcggcc caagtcatag ccaacttcgg cgccggacac gaacagtcgc
     3421 ccgccggcat tgagatagcc cgtcaccgcg gtgacttcac cggagctgaa agtttcatcc
     3481 acggtggact cttcgcccaa catccagacc accttggcat aattggtcag caccgcagcc
     3541 cccgtcacga gggtgtggtc cataaaatca aacgtctgcc cggctgctgc cagcgcaagc
     3601 ccgtgttcct tcacgtaatc gccgctgttg atcatcttcg ggcgcaccag agtcacttga
     3661 ccgttgagtc cattggcaaa ataacgggtg ggcgccaaag agctgtcatt gcggtcaaag
     3721 cccgccacca ccagcacgcc cgccttggaa ccaccggccg tcacgcgcac gccggccacc
     3781 tgcgagttga gcgactcgcc gcccgcattc gtcgcgcata cacggaagaa aatcgtcgcg
     3841 ccggcattca ggttggtgat ggtcttgctg gtagtcgccc cgccggaaac cgtgattggg
     3901 ttgccgaagc cgcgaccgtc cgttgaagta taaatgatga agccttgcgg cacgccgcca
     3961 ctggccgccg tgcgggtggg catggcccac gacaccgtca ccgcccccgc ccccgaatta
     4021 gccgccttca atttgatggg ccggtccggc gcgttggtat tgatgatgcc gctggcgtaa
     4081 tatccgctca aatgcttgat gagcccgcgc gtgcttgagc gcgccagcca ttcgcgcccg
     4141 gcgggggttt tcagaacggt acagtcgtcg gcgttgtcat ggaacgccac ctcgttgatg
     4201 gtggtgtcca tctttttgaa cacatattca ttgaaggcat tgtacaactc gccataggtg
     4261 ctgccgtaat agtagctggt gcgtgaactc caactgggga tgacgccatc ggtcacgcca
     4321 gcacgcatgt cttcgttgca ctgccgcgcc aaggccacgg ccagattggt ttgcgcttgg
     4381 taaaacgacg gatagttggc ttggatgcgg gtgtcgtaaa ggccaatgga gccgcgtgcg
     4441 ccggctccac cgcccgcatt ggagtggaag ccaacataca cccgccgcca gcgtgggcgg
     4501 tcattggtgc ggcacatcag gtccgccatg cgcgcgggct ggccgatgtt gtcgttgcgg
     4561 tcgtcataac cggccatgtc atacaaggtg ctggggatcc cctgcccgat ggacttgatc
     4621 acccagaagc ggctggattc caactccgat tcaaacccgg aaactccggc cgatccacgc
     4681 gccgtggtgc ccatgccgtt gccgaaacga atggcgtcgg caatcacgtt gaagccctct
     4741 tgtccccccg gcacataatt gctgatgttc acacaaccgt tggtgccctt ggcgaagtga
     4801 taggtcccca gccaaaccca gccgcaaccc accgcgctgt ggtccacccg cacatccgtc
     4861 accccgccgc tgtgataaat tcggtaaagc tggttcgtcc ggttaccgct ttgcagcacc
     4921 caggtataaa ccggataatc ccctgccgcg ggcagattcg ggcggtacgt cgcccatgcc
     4981 gtggtttccg aggaattgac cgccgcgtat cggtacgaca ctgcatcgcc gctcttgccg
     5041 taataaacgc tgttggtcga gttgaaccaa gtgccgccaa acgtcacctg cccgcgcgcg
     5101 gtcaggttgg tgtccatgtt gtcgagcacc acctcgttgg tctggtagcc caccgggcgg
     5161 aacggaacca ccgtggcgcc cgccttccaa gcggattccg caaagtagtt gagctggtca
     5221 atgttgccca tgtcctcatt caccgcgttc agcagggggc gttcgggaat ccacgcggtc
     5281 aacgcttcat tcgcgccgaa accgtggccg gcgctggtga acaccaccac gccggagagt
     5341 ggccccgtcg gctgcttggc ggccacttgc ggcaggccgg tctcatcggg cgtggtcacc
     5401 ggcaaatcgc caccgccggt ttcgccgctg cccccctcgt cgcctggttc gccgctctcc
     5461 tgcccggcca tggtcccctt ggtcaggatg gtgccattga agcccacgga cacaaactga
     5521 tccgccgtgt agctcatgcc acgcagatcg ttggtagtcc ccgaggtttc cgcgctccaa
     5581 gtcaggccgt cagcggaggt ccaaatcacg ccgtttttgc ctgccgcgcg gaacgcgccg
     5641 gagcagtagg ccacgcgata caggtacacc gagttggtgg tgaacgacca tgttaggcca
     5701 tccgcggagc gtagcgtgcg cgtgttgccc acggccacat agtagccatt gccatacgtc
     5761 acatccgaca aatagactgt cgtgcctgaa gtccgtttgg tccacgccac gccgttggtg
     5821 gaagtgagaa tggttccgtt ggcgccagaa actacaaaca ggggcgaagg cgcggtcgga
     5881 ttgttggtgt cgcccgtgct aatgaactcc gcgccgtacc ccaccccttg cagtgaatcg
     5941 ctcacccccg aggtcacgct gctccatgtg atgccgttgg tggagcggat aatcgccccg
     6001 ccatcaccac aggccacata gatgccgttg ccatacgcca cgccgcggaa cagggcattg
     6061 gtgggcgatg tccggctaac ccagttcagg ccgttggtgg aggttagaat ggcaccgccc
     6121 tcgcccacga tgacccagag cgggccatcg ccattcacat tcatcagcag gccgctctta
     6181 ccgctggtgc gagcagtcca gttgatgccg ttggtggagg tataaatggc accgcccgcg
     6241 ccgctggcca cgtgcagtcc ggcacgactg cccacgccat agaaattggc cgtatgcgcc
     6301 gaggtgcggc tgacccacgg cgcggaggcc gacccgcccg gatcgccact gcccggatca
     6361 tccggcggcc ccgcatccgc gccgaatttg tcggcaatca gcgtccacaa ttcagtttgg
     6421 ccggtgccct gcgtcagatt ccaaatgcca atccccccca gccccttggt gttggctaaa
     6481 tcatacttca tgcccaagct gagcgtatca tcataaaaac attgataggc tgtgctgttc
     6541 gaggtgtaca cgtaataagg cacgctggcg ttatcgttcc aacgccggcc atacttgttg
     6601 gcctccgatt tggccgcgcc ataggtcaac gccacggaat aactgctgcc cagactggcc
     6661 gcgccgaggt tggtgctggc cgccgcccag cgtcgcccgt aatacggcac ccccagcatc
     6721 aatttgctgg ccgggaaacc tttgcccaaa tagttggtga tggaataatt gtccgaacac
     6781 cacgaattgg cgccaaccca ttgggcactg ctgaacagcg gcgccaccgg ccccggcgtg
     6841 ctgctgccag aataataata gtcgtagccc atgataatcg cgtaatccat ccccgcggta
     6901 ttataggctg acacattaaa attggcgctc cagtccactg ccggcagcgc gatgctcact
     6961 tcatagtttg gattcgcgtc atggaatttc ttgatcaggt tggtcatgaa cgacgtcatc
     7021 gccgcgccgg agccgctcca tgacccgatg gtttcaaagt caatgcacac gccatcgcct
     7081 gccccccccg cccggttggt catggccact aacaggttgc ggatcaaatt ggttgtagcc
     7141 gccggattgc tcaaaaactg ctgattgttc gccgagccga acagggtgac cgtcaacacc
     7201 gcctttaccc cgttgctatg cgcccaactc accatcggcg tggtgttcca gccgtgcatg
     7261 tcggtcgcgt taccattggc gggattgacc tcgtaggaaa agaacgccag atgggtcaga
     7321 ttggaaaatt ggtaatttgc caaatcgctg gatgtcgccc agtacggatg ccatcccaga
     7381 acccgtttgc cggcggcaat ctgcggcgcg cccaaaatgc cacgcgcagt gggccggcgg
     7441 gatggcaggg gcgcctcttc gccactggta tcccccggtg gctcgccggc gtgggcctcc
     7501 aacgccaact ggtgttcgct cttgcgttca tccaagtcgt ccggaatgag ccaagccgcc
     7561 tgttctactt cgtcaggact ggcgcccgtc acccgcgcct gcgaccgtgc caaacgccgc
     7621 gcttcggcca ccagcggcgt ttcatcctgc gcctccggca acacgaaatc ggtcacgatg
     7681 tccggcccgg tttcatccca ggcgttttgg ccggcaaccg cattttcttc ctgcggtgtt
     7741 gccagacgtt tggcgcgccg agccggctcc ggctctggat ccgcccatgc caatccggcc
     7801 gccagcaaca atgccatgcc gcccatgccc cagaccaatg agaacaagtg tttattgttc
     7861 atgatttgcc aactctgcta aagcctcttt cccccgtcaa ggctttctcc aggaaaaatt
     7921 gcgattgtta agatttgatt aataataatc tggggccatt tttttattat ttttttgttg
     7981 cataggattt gacccgatca ctactatatc ttccgaaagg ctggcgtgaa acttgacgca
     8041 tcattcgcca acgcccgccg gtcttcgcca aggaggtaga catgagcacg ttgtggactc
     8101 gcaggattct ggctctggtt gtaatcatgg ggatttgttc agccgctacc actttcgcgg
     8161 caaagaccaa acccaaaaag gaagcgcctc ctccggaggt tagcgaaact gaggcgccag
     8221 ccttcccgcc ggccgagttg accgtgggct tccaagcgcg cgactccgaa accgagggca
     8281 tcggtgacct gctggtcccg gtgtggaatc ccggcggcgc ggggctgctg ttcctcaacc
     8341 cacgcacggc catcaccgac cacgatggcg aagaagccaa tatcggcgtg ggctaccgtc
     8401 agcttctccc caagtggaat gtcattcttg gcgccaatgc tttctatgac taccgcgaca
     8461 ccggcgccta caactatcac caatggggtt tcgggttgga actgttgagc caatggctgg
     8521 atgcccggtt caactattac gatccggaag acaagacgta tgtggccgcc accaccaccg
     8581 aaacctccac gcgccagagc gtgcgcgcca caacccggtg ggaagatccc tacgccaccg
     8641 gccatgaagt gcgccagaac ctgttgacca tccgccaact caccaccacc accaccaccc
     8701 gcaccttcga gttgtttgag cagccttacg gtggccacga ttgggaaatt ggcctgcgcc
     8761 tacccatcaa ctgcaacggc tttgaggcgc gcctcttcgg tggctattat gatttcgacc
     8821 gcgatttcgg cgccgccgcc cgcggctgga aaactcgcgc ggaaatccgc ctgcgctcca
     8881 gcctcttcct cgatgccggc ctctacgaaa acgacaaact taccggcagt gactggtatc
     8941 tcggcgctcg cttgaccacg ccgcttgact tggctcaact ggcgagcggt cgcaatccct
     9001 ttgccactgc caaagcccgc tggagtggcg aacccgccga tttggcttcc cgcctgaccg
     9061 agatggtcat gcgcgacccg caaatccgct tggagcactc caagttcatc gagaaccccc
     9121 aactcaggac agaaaccgcc accaccacaa aaactgccac gcgcaagcgc catgtcctgc
     9181 tgcccgatgt gcagttcgtt aatggcgatg ctcccgccgg cggcgatggc accggcgagc
     9241 ggccgttcaa ccagattcaa cagggcgcgg actcggtttt cggtgaccgc aatgtgtacg
     9301 tctataatgc cagcggccct tacatggaaa acgtggtgtt gcaagccggt accaccctct
     9361 gcggcagcgg tgtccagatt cccggctatg gcggcaaaac cttcggcagt ggcatcgcgc
     9421 cggtggtaga tggcaacaac cttggcccgt ccatcaccgt ggccaaccaa accacagtca
     9481 agggattcag catccgcaat accgatcgcg gcctgccgcc catcattttt gacacgcccg
     9541 ccttttacaa tgtcaatccc gcccgcatgg gcatctttgg caatggggcg acagacgtga
     9601 ccatcacgcg caacctgctg gccaataatg agactgccgc ccacttcagc cgcattggct
     9661 cgttccggct gaacttctcc gataacactg tcgaagataa cagccttgtc ggcttggaaa
     9721 tttgcacggc gggcgccggt ttgccttcca gcgatacctg cgaggccacc gtagcgcgga
     9781 attccatcca gaataatggc agcatcggcc tctattcaac catgtggaat taccagagct
     9841 cgctcctgga cattgccgat agcgacatca gccggaatac cgttggcgtg tcaagttccc
     9901 aagcggaatc gggggacgcg atattgatcg tccgcaacct ccatgcggat cacaacgcca
     9961 tcgatggact tctcgcggag caaatcctaa acatcaataa cttaatcgcg gttgccggca
    10021 gctcgttcga tcacaacact ggctatggcg tgaattccga tcagagggct gaggaaaatt
    10081 ctttggcctt tttcggcatg ccagagggac ttgatcaact cgtgaacgcc gtcggtgctt
    10141 ttggcggacc ggtgcctgct ttcctggcgc cgctgctggc ctcggcgggc ggagtcagcg
    10201 ccagccataa tggtcgtggt ggcattggct gcggcattta tgcccaaaac gacgcggcct
    10261 tgggcgcatt ctttgacgtc acggccaacg ggaacgaatt tagcggcctc gtcgccgaga
    10321 ttgtcagcga tggcaatctg gccgtcggtt tggcgggcgg aagcggcaca ttgaataact
    10381 tactcaacct gggcagcgat gcggtggccg gaatgctggg cattgatctg ccggttctgg
    10441 cactgggcaa cggcgaaatg caggtcaatg acaattcgag cttcggcatg ttaatggcag
    10501 tgcgtggcga taccgcgcta gccgccgtgg ctggggtcac ggcgaatggc aac
//
LOCUS       DAOPNP010000088        10493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_7212_length_10493_cov_9.080475, whole genome shotgun sequence.
ACCESSION   DAOPNP010000088 DAOPNP010000000
VERSION     DAOPNP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7212_length_10493_cov_9.080475"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..10493
                     /locus_tag="PKI64_08235"
     CDS             129..10493
                     /locus_tag="PKI64_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOE01033.1"
                     /translation="MKTRFNKTVWVTSALALVLSASLASAAHILQTFFIPLPEDDVEV
                     SLKIIDSFGPNIGTEMQSIIGIAVGSDNTVIYYDHWEDGYEADLTSPTQLSTQIWGDG
                     DPSNGYPPGFPDDVLMAGSVIELSAVIDVTRNSVTIEYDGRDKLASTYPIAVTRAMYP
                     VEPGEVIAEAVGVRDIGTHGMLYRVPVGEGTGVGAHTNEMFSSSGLYIMGDYDNTRVE
                     IDADGDGIFETTVYLDQGEPYTVNSGVKAGGTVRGNQPFQCTLVTGDIGSNYETRWFD
                     LWPENQWDNDYFTPVCSRSATYTADVYLFNPNNTAITVTVVTATSTSSIPIAANTTGA
                     PFRMPVNSGARLFTADGQPFIGVSIFDTSGTCQAYDWGISLVPKKMMTTAGLVGWGPG
                     YGSTGTGDNGSPVWVSALSNTTLYVDLDGDPLTGPLVDPHGNHYDFATNLTALQVVAL
                     RDNSDNDQTGLRYYTLDGTVVLGAWGVDPQYAGTGNPYLDMGYAIPAYPAVVSRKNAA
                     LLIDVNGNGFPDAGDSLEYEIDVVNVGFASASHVIFEDDLPTNLTTYVSNSAMIAVAG
                     VTNIIPDSLPPKLTRFPFDEGGYDVGTVAIGTTTKVRYVTQIVTNLPADFTGYIHNNA
                     TVGGTNGTWTTGSTTNILVRGLTINKAVNTTNILEPGSNLTYTIKVVNTGAVAYTGLR
                     IQDELPLGLEYVPNSTVVTVSGARTNTVMDRFDERTYTNSNGTIPWLTPWVESGESDG
                     VAGGNVQVLGNFMTNSAEVYVLQVANANRAATRAANLAGATSATLSFRYRRVSMESGD
                     AVTVLISTNNWATSTTLASLAGAAPGSLTDTSFLATNYNVSAYISTNTAIRFQANGAM
                     AADDYVFFDDVTFTLVSSNAAVAGQPPPNLVDNLTLMPGSTATVSFSATVLSPPLATQ
                     VVNRAQTSADQQREPVESNPVTNAIRATAGVVLQKTSGTTNLLVVGTSVVYTIQISNT
                     GTVAQTGIRLEDLLPAGMTYSNGTAKLYRPFLHTNVFLDQFDLRQYDNSDGNVAWSGP
                     WTEVGEDGNPQSGNIQIAVDNGSVPGQTYALRTVGTTAVQRAASLAGYTNVSVAFDYR
                     RTGLRATNYVVVYASANNGATFTEVGRIGGATNDGSYFPASFNISSYASTGTVIRFAG
                     STGRGTTNIVWLDNIRILAAGARATNALPDPPLLLEGYTLPPGTNMTVYLTATVDSPL
                     QSTNFVNTARLQSDQHPDWLTATATNRAAGTVGMTLTKTAQLAGNWNLGETNVYEIVL
                     ENTGTVAQTGVYLTDVLPPGTKYVPNSVTITQQPAPAFTTQLFTTAGTATFTPPASWV
                     TSVVVMAWGGGGGGGTRTSNGRTGGGGGGAFSMSTVSVVPGSNYTVTVGAGGASSTAG
                     GASWFSLGNTTNVLANGGSGVPQNTTTGGAGGATNGGIGTVLYAGGRGANSSGTYGGG
                     GGSSAGTNATGNNATSATGATAPAGGGNGGNGATANGNGNNGNQPGGGGGGAFRSSGT
                     RYGGAGADGQVLVRYLSSPPLVPGAPPNLLSNVTIRPGEKVTIRLAATLTLPLVSTQF
                     VNMATVTSDQQPPRSASVTNVSVLNSVGDRTWYDLNNNGIQDAGEPGLTGVAVRLYSS
                     TSNLLLTTTSTVAGAYAFTNLPSGSYFLEFVTPQDFTPTLQDQGGNDALDSDIAPATG
                     RTAVFTLSGGANDTTRDAGYYQPPASVGNFVWRDVNGDGLQSGGSETGLPGVVVTLYD
                     ATSNTVGVTTSSAAGAYAFTNLPPGNYFLHFAAPNGYTYTLPDQGGNDAVDSDASPFT
                     GFTELFYLGPGTNDTTRDAGFVAIVRGLTLSKTASITNECLAGGMTNTYTIVLQNTGN
                     VAQAGINLQDLLPAGLTYVSNSVNVTLGVAPAQLVTTTYTGSGTFTVPTNVTLATVQA
                     WGGGGGGATRSTTGGGGGGGGGAYSREMLNLQSGSNYTVTVGAGGAAGNNGGDSWLSL
                     GSVTGVLARGGSGVAQNTATGGAGGATNGIGRVQYVGGNGADAGGTNGGGGGSSAGTN
                     AAGNNATSATGATAPAGGGNGGNGRSGTSGAGTAGTAPGGGGGGAYRTSGTQTGGAGA
                     AGRVTITYTADAGGYLGAPPNLLTNGTLAPGASLTITFQAVVSTPPPAGALTNRASVY
                     SSTQPAISASVTNCVVSADVGVQKLVTTNQPFSAQIIEYILVATNSGPSTATGIKITD
                     LLPSQVQYNSHSNGTYNNVSGVWTIGSLAVNTATTLYINVTVREGYEGRGITNTARIS
                     EREVYDPNPNNDTNSVVIVPKAGKIGDFVWEDLNMNGQQDPGEPGVAGVVVRLYNAAS
                     NVIGTTTTTVSGAYLFTNVPPGSWSVGFTPPAGYVFTGKDVGDDATDSDADPATGRTG
                     YITLGVGGENFTLDAGLYRYAAVGDRVWFDANLDGIQNATETTGIGNMTVQLLDRNSN
                     LVATTTTDAAGAYLFTNVVPGSYFVRFNLNPIMAHSWITAAKQAGNDVVDSDGLSLLP
                     NGYSWTALLNLASGQTNRNVDLGIRPQKATRATLSEVWGEWRDGAGRVVWRTEAEWGT
                     AGFVLYRVNPDTGAEEQLNTEAVPATLDGLGGIYEWVDPAAQEGARATYRLTEIEFAG
                     TVQDLGTHEVVFGAPPAAAFAARAEVAAAKAVRRLRKPAALLPAAESSSILKVQYRDA
                     GLYAVELAAIAAAMDRPVDEIAAHAATNGLALTQQGAPVPYIFDNARARVVFHGPATT
                     NWYARDNAVLIALGAGQAMPRRAPAAASGANVTAQRLHFEQDRYPFDSALEKPADFYY
                     WDYLIGGTGPHSLKNFPLDLSGCRGAVELTVRLQGWSRSTNTPDHSAELRWNGAVIAA
                     TTLDGAQTKDVRVTIPAGDVADGINTLAIKAYLLPGIVSSAFVLDWVEAELPREITPQ
                     AGTTYLLADGQTALAATAFAAPLAVALNAAGQPTWLADDAGELPAKAWAVAAGDARYA
                     LAEAAGIPALTPEPIADNPWFLAATNRVDYLIISSRALAAAAQELADYRAGQGLRVGL
                     ATFEDACDWFAGGLRTPEAIPALLRHAHTVWAEAPWLVALAGNGHYDYLNALNLEANH
                     VPPLLTASYEGLFAADGLLADWTGDGQPDVALGRLPARTPAELAAMIAKIKAYEQDFG
                     HAWENQIVLAADAADAAGDFTAVNAQLAALAGAEYPVATVDLAAQGLAPARAALLNWF
                     GSGAGIIHYTGHGNVNSWSGKGLLKAADVNALNNARQPVVVALSCLIGRYEAPGVQSL
                     GELLMQRAAGGAVAVWGPSGLSRNHPAAELGAAFYDLVLQQGAGTLGLAVQQARAALP
                     AETFTRDTFAIYNLLGDPALRMAGNTGAGRTAASFAQWRWAQYAPHELNDPATSGPTT
                     HNFQLYAMDGAGELVAELPEFGYAIPDEAAGGGFIMRWQRRVNRPDLDYHFYFSPDLV
                     TWSNITTGTETLSVEPAPDGIMETVRTRVNLPAAENIFLGVKAVRK"
BASE COUNT         1907 a         3411 c         3489 g         1686 t
ORIGIN      
        1 tcctgtgtcc caaggagtta tgactttcca tttcagaggt gtcgaactca ggaaattatg
       61 ccgataaatt gttccagatt agcagaaata tcggtgtgcc ccaaaaggtc aaccgattca
      121 atgggattat gaagacacga tttaacaaaa cggtgtgggt aacgtccgcg ctggcgctgg
      181 tgctatcggc ctcgctggcc tcggcggcgc atattctgca gacgtttttc attccgctgc
      241 cggaagacga cgtggaagtg tcgttgaaaa tcattgattc cttcgggccg aacatcggca
      301 cggagatgca gtccatcatt ggcatcgcgg tggggtcgga caacacggtg atttactacg
      361 accactggga agacgggtat gaggccgatc tgacgtcgcc gacgcagctt tcgacgcaga
      421 tttggggtga cggggatccg agcaacgggt atccgccggg tttcccggat gacgtattga
      481 tggcggggtc ggtgattgag ctctcggcgg tgattgacgt gacgcgcaac agcgtgacga
      541 ttgagtatga cgggcgggac aagctggcct ccacgtatcc gattgcggtg acgcgggcga
      601 tgtatccggt ggagccgggg gaagtgatcg cggaagcggt gggcgtgcgc gacattggca
      661 cgcacgggat gctgtaccgg gtgccggtgg gcgagggcac gggggtgggc gcgcacacca
      721 acgagatgtt cagctcttcg gggctgtata tcatgggcga ctatgacaac acccgcgtgg
      781 agattgacgc ggacggcgac ggcatttttg aaacgacggt atatctggac cagggcgagc
      841 cgtacacggt gaatagcggg gtgaaggcgg gcggcacggt gcggggcaac cagccgttcc
      901 aatgcacgct ggtgacgggc gacattggct cgaactacga aacgcgctgg tttgatttgt
      961 ggccggagaa ccagtgggac aacgattatt tcacgccggt ttgttcgcgg tcggccacat
     1021 atacggcgga tgtgtatctg tttaacccca acaacacggc catcacggtg acggtggtga
     1081 cggccaccag cacaagctcg attcccattg cggccaacac cacgggcgcg ccgttccgga
     1141 tgccggtgaa cagcggcgcg cggttgttca cggcggacgg gcagccgttc atcggggtga
     1201 gcatctttga caccagcggg acttgccagg cctatgactg gggcatcagc ctcgtgccga
     1261 agaagatgat gaccacggcg gggttggtgg gctgggggcc ggggtatggc tccaccggca
     1321 cgggtgacaa cggcagcccg gtgtgggtgt cggcgttgag caacaccacg ctgtatgtgg
     1381 acttggacgg cgacccgctg acgggcccgc tcgtggatcc gcacggcaac cactacgact
     1441 ttgcgacgaa cctgacggcg ctgcaagtgg tggcgctgcg ggataactcc gataacgacc
     1501 agacgggatt gcgctactac acgctggacg gcacggtggt gctgggggcg tggggcgtgg
     1561 acccgcaata tgcgggcacc ggtaatccct accttgacat gggctacgcg attccggcct
     1621 atccggcggt ggtgtcgcgc aagaacgcgg cgctgctgat tgacgtcaac ggcaacggct
     1681 tcccggacgc gggcgacagc ctcgaatacg aaattgacgt ggtgaacgtg gggtttgcct
     1741 cggcctcgca cgtgattttc gaagacgacc tgccaaccaa cctgaccacc tatgtgagca
     1801 atagcgcgat gattgcggtg gccggcgtga ccaacatcat tccggacagc ctgccgccga
     1861 agctgacgcg cttcccgttt gacgagggcg gttacgatgt cggcaccgtg gcgatcggca
     1921 cgaccaccaa ggtgcgctac gtcacgcaga tcgtgacgaa cctgccggcg gacttcaccg
     1981 gctacatcca caacaacgcg acggtgggtg gcaccaacgg cacgtggaca acgggctcga
     2041 ccaccaacat cctcgtgcgg gggctgacca tcaataaggc cgtcaacacg acaaacatcc
     2101 tggagccggg cagcaacctg acctacacga tcaaggtggt gaacaccggc gcggtggcct
     2161 acacggggct gcgcattcag gacgagctgc cgttgggcct ggaatatgtg cccaacagca
     2221 cggtggtgac ggtttccggc gcgcgcacca acacggtgat ggaccgcttt gacgagcgaa
     2281 cgtacaccaa cagcaatggc acaattccct ggctgacccc gtgggtggag agcggcgaaa
     2341 gcgacggcgt ggcgggcggc aacgtgcaag tgctgggcaa cttcatgacc aactcggcgg
     2401 aggtgtacgt gctgcaagtg gccaatgcca accgtgcggc cacgcgcgcg gcaaacttgg
     2461 ccggcgccac gagcgccacg ttgagcttcc gctaccggcg ggtcagcatg gagagcggcg
     2521 acgcggtaac ggtgttgatt tcaacgaaca attgggccac ctccacaaca ttggcctcct
     2581 tggcaggtgc ggcgccgggc agcctgacgg acacgtcgtt cctggccacc aattacaacg
     2641 tcagcgctta catttcgacc aatacggcca tccggttcca agccaatggc gccatggcgg
     2701 ccgatgacta tgtgtttttt gacgatgtga ccttcacgct ggtgagcagc aatgcggcgg
     2761 tggccggcca gccgccgccg aatctggtgg ataacctgac gctgatgccg ggcagcacgg
     2821 ccacggtgag tttctcggcc acggtgttga gcccgccgct ggccacgcag gtggtcaacc
     2881 gggcgcaaac ctccgccgac cagcagcgcg agcccgtcga atccaatccg gtgaccaatg
     2941 ccatccgggc cacggcgggc gtggtgctgc aaaaaacgtc cggcaccacc aacctgttgg
     3001 tggttggcac cagcgtggtc tacaccattc agatcagcaa caccggcacg gtggcgcaga
     3061 cgggcatccg gctggaggac ctgctgcccg ccggcatgac ctattccaac ggcacggcca
     3121 agttgtaccg cccgttcctg cacaccaatg tcttcctgga ccaattcgat ctgcgccaat
     3181 atgacaacag cgatggcaac gtggcgtggt ccggtccttg gacggaagtg ggggaagacg
     3241 gcaatccgca gagcggtaac atccaaatcg cggtggacaa cggctcggtg ccggggcaga
     3301 cctacgccct gcgcacggtg ggtacgaccg ccgtgcagcg cgcggcgagt ttggcgggct
     3361 ataccaacgt gtcggtggcg ttcgattacc ggcgcacagg cctgcgggca acaaactacg
     3421 tggtggttta tgcctcggcc aacaacggcg ccaccttcac ggaggtgggc cggattggcg
     3481 gggcgacgaa cgacggctcc tatttcccgg cctcgttcaa catttcgtcg tatgcctcga
     3541 cgggcacggt gatccggttt gccggctcga ccgggcgggg caccacgaac atcgtttggc
     3601 tggacaacat ccggattctg gcggcgggcg cgcgcgccac caatgcgctg ccggacccac
     3661 cgctgttact ggagggttac accctgcccc cgggcaccaa catgacggtc tatttgacgg
     3721 ccacggtgga tagcccgctg cagtccacca actttgtcaa tactgcgcgg ctgcagagcg
     3781 accagcatcc ggattggctg acggccaccg ccaccaaccg ggccgccggc accgtgggca
     3841 tgacgttgac caagaccgcg cagttggcgg gcaactggaa tctgggcgaa accaatgtgt
     3901 atgaaatcgt gctggagaac accggcacgg tggcgcaaac gggggtgtac ctgacggacg
     3961 tgctgccgcc gggaaccaaa tatgtgccca acagcgtgac catcacgcag caaccggcgc
     4021 cggcgttcac cacgcaattg ttcaccacgg cgggcacggc caccttcacg ccgccggcat
     4081 cgtgggtgac ctcggtggtt gtgatggctt ggggcggcgg tggtggcggc ggcacacgga
     4141 cctctaatgg gcggactggc ggcggcggcg ggggcgcatt cagcatgagc accgtatcag
     4201 tagtacccgg gagcaactac accgtcacgg tcggcgcggg cggtgcatcc agtacggcgg
     4261 gcggagcctc gtggttcagt ttgggcaata ccaccaatgt gctggccaac ggtggcagtg
     4321 gtgttccgca aaacacgacg actggcgggg ccggcggggc gaccaatggc gggattggca
     4381 cggttctgta tgccggcggt cgtggcgcga attcaagtgg cacctatggc ggcggtggtg
     4441 gctcttcagc cggcaccaat gccaccggca acaacgccac cagcgccacg ggcgccacgg
     4501 caccggcggg tggcggtaac ggcggcaatg gcgctacggc gaatggcaac gggaataatg
     4561 gaaaccagcc gggtggcggt ggtggcggcg cctttcggtc aagcggcaca cgctatggcg
     4621 gcgcaggcgc tgacgggcag gtgctggtgc gctatctcag ctcgccaccg ctggtgccgg
     4681 gtgcgccgcc caacctgctc agcaacgtca ccatccgccc gggcgaaaaa gtgacgattc
     4741 ggttggcggc caccttgacc ttgccgttgg tttcgaccca gtttgtgaac atggccaccg
     4801 tcaccagcga ccagcagccg ccgcgttcgg cgtcggtcac caatgtttcc gtgttgaact
     4861 cggtggggga ccggacgtgg tacgatctga ataacaatgg cattcaagac gcgggcgagc
     4921 cggggctaac cggcgtggcg gtgcggctgt atagcagcac atccaatttg ctgttgacca
     4981 ccacctccac cgtggcgggc gcttatgcgt tcaccaattt gccgtcgggc tcgtacttcc
     5041 tggagtttgt cacgccgcag gatttcacgc ccacgttgca ggaccagggc ggcaacgatg
     5101 cgctggacag cgacattgcg ccggccaccg ggcgcacggc cgtgttcacg ctgagcggcg
     5161 gcgccaacga caccacgcgc gatgccggct actaccagcc gccggccagc gtgggcaatt
     5221 ttgtctggcg cgatgtcaat ggcgatggcc tgcaaagcgg cggcagcgaa accggcctgc
     5281 cgggagtggt ggtcacgctg tacgacgcga cctccaacac ggtgggcgtc accacctcgt
     5341 ccgcggccgg cgcctatgcc ttcaccaatc tgccgccggg caattacttc ctgcattttg
     5401 ccgcgcccaa cggctacacc tacaccctgc cggaccaagg cggcaacgat gcggtggata
     5461 gcgatgcttc gcccttcacc ggctttaccg agctgttcta tctgggcccg ggcaccaacg
     5521 acaccacgcg cgacgccggt tttgtggcca ttgtccgcgg gctgaccctc agcaaaaccg
     5581 ccagcatcac caatgaatgc ctggccggcg gcatgaccaa cacctacacg attgtgctcc
     5641 agaacaccgg caacgtggcg caggcgggca tcaatctcca agatttgctg ccggcggggc
     5701 tgacctatgt ttccaacagc gtcaatgtga ccctcggcgt ggcgccggcc cagttggtca
     5761 ccaccaccta tacgggctcg ggcaccttca cggtgccgac gaacgtgacg ttggctaccg
     5821 tgcaggcgtg gggcggcggc ggcggtggcg ccacgcgcag caccaccggc ggcggtggcg
     5881 gtggcggcgg cggggcgtat agccgcgaga tgttgaattt gcagtccggc agcaactaca
     5941 ccgtcacggt cggcgccggc ggcgcggccg gcaataatgg cggtgattcg tggctcagct
     6001 tgggcagcgt caccggcgtg ttggcgcgcg gtggcagcgg cgtggcgcaa aacactgcca
     6061 ctggcggggc gggtggcgcg accaacggca ttggccgggt tcagtatgtc ggcggcaatg
     6121 gcgcggatgc ggggggcacc aatggcggcg gcggcggttc ttcggccggc accaacgcgg
     6181 cgggtaacaa cgccaccagc gccacgggcg ccacggcgcc ggcgggtggg ggcaatggtg
     6241 gcaacggacg tagcggcaca tccggcgcgg gcaccgcggg caccgcgccc ggtggcggcg
     6301 gcggcggggc ctatcgcact tccggcacgc agactggcgg cgcgggcgcg gcggggcgcg
     6361 taacgattac ctacaccgcc gatgccggcg gttatctggg cgcgccgccc aacttgttga
     6421 ccaacggcac gctggcgccc ggcgcgtcgc tcaccatcac gttccaagca gtggtgagca
     6481 caccgccgcc ggcgggagcg ctgaccaacc gcgccagcgt gtatagctct acccagccgg
     6541 ccatcagcgc cagcgtcacc aactgcgtgg tgtcggcgga tgtgggtgtc caaaaactgg
     6601 tgacgaccaa ccagccgttt tcggcgcaga tcattgaata cattctggtg gccaccaaca
     6661 gcggccccag tacggccacc gggatcaaga tcaccgacct gctgccgagc caagtgcaat
     6721 ataacagcca ctccaacggc acctacaaca acgtcagcgg ggtttggacc atcggctcgc
     6781 tggcggtcaa cacggcgacc acgctctaca tcaacgtgac cgtccgcgag ggctacgagg
     6841 gtcgtggcat caccaacacc gcgcgcatct ccgaacgcga ggtgtatgac cccaatccga
     6901 acaacgacac caactcagtg gtgatcgtgc ccaaggccgg caagatcggc gactttgtct
     6961 gggaagacct gaacatgaac ggccagcagg accccggcga gccgggcgtg gcgggcgtgg
     7021 tggtgcggct gtataacgcg gcctccaatg tgatcggcac caccacgacc acggtcagcg
     7081 gcgcctacct gttcaccaac gtgccgccgg gttcgtggag cgtcggcttc acgccgccgg
     7141 cgggctatgt gttcaccggc aaggacgtcg gcgacgatgc caccgacagc gatgccgacc
     7201 ccgccacggg ccgcacgggc tacatcaccc tcggcgtcgg cggggaaaac ttcacgctgg
     7261 atgccgggtt gtatcgctac gcggccgttg gtgaccgcgt ctggttcgat gccaacctcg
     7321 acggcatcca gaatgccacc gaaaccaccg gcatcggcaa catgaccgtg cagttgctgg
     7381 accgcaactc caacctggtg gccaccacca ccacggacgc ggcgggcgcg tacctgttca
     7441 ccaatgtggt gcccggctcg tactttgtcc gcttcaactt gaaccccatc atggcgcaca
     7501 gttggatcac cgccgccaag caggcgggaa acgatgtggt ggatagcgac gggctctcgc
     7561 tgctccccaa cggctactcc tggaccgcgc tgctcaacct ggccagcggc cagaccaacc
     7621 gcaacgtgga cttgggcatc cgcccgcaaa aagccacgcg cgccacgcta agcgaggtgt
     7681 ggggcgaatg gcgcgacggc gccggccgcg tggtgtggcg cacggaagcg gagtggggca
     7741 ccgcgggctt tgtgctctac cgcgtgaacc cggacaccgg cgcggaagaa caattgaaca
     7801 ccgaagccgt gccggccacg ctggacggtt tgggcggcat ctatgaatgg gtggaccccg
     7861 cggcgcagga aggcgcgcgc gccacttacc gcctcacgga aatcgaattt gccggcaccg
     7921 tgcaggacct cggcacgcac gaggtggtgt tcggcgcgcc ccccgccgcg gcgtttgccg
     7981 cgcgcgcgga agtggccgca gccaaggccg tgcgccgcct gcgcaagccg gccgcgctgt
     8041 tgccggccgc ggagtcgtcc tccatcctga aggtgcaata tcgcgatgcc gggctctacg
     8101 ccgtggagtt ggcggccatc gccgccgcca tggaccggcc agtggatgag atcgcggcgc
     8161 acgccgccac caacggcctc gccctcacgc agcagggcgc cccggtgcct tacatatttg
     8221 ataacgcccg cgcgcgcgtg gtattccacg gcccggccac caccaactgg tacgcgcgcg
     8281 acaacgcggt gctgattgcg ctgggcgccg gccaagccat gccgcgccgc gcccccgctg
     8341 ccgccagcgg cgccaacgtg accgcgcagc gcctccactt cgagcaggat cgctacccct
     8401 ttgacagcgc attggaaaaa ccggccgatt tctactactg ggattatctg atcggcggca
     8461 cggggcccca ctcgctcaag aatttcccgc tggacctgag cggctgccgc ggcgcggtgg
     8521 aactgaccgt gcgcctgcaa ggctggtcgc gctccaccaa cacgccggac cactccgcgg
     8581 agctgcgctg gaacggcgcg gtgatcgccg cgaccacgct ggatggcgcg caaaccaagg
     8641 atgtgcgcgt gaccatcccc gccggcgacg tggcggatgg catcaacacg ctggcgatca
     8701 aggcctacct cctgccgggc atcgtcagta gcgcgtttgt gctggattgg gtggaagctg
     8761 aactgccgcg cgaaatcacg ccacaggccg gcaccactta tctgctggcg gatggccaaa
     8821 ccgcgctcgc cgccacggcg ttcgccgcgc cgctggccgt ggcgctgaat gccgccggcc
     8881 agcccacctg gctggccgat gacgccggcg agctgcccgc caaggcgtgg gccgtcgccg
     8941 ctggcgacgc acgctacgcg ctcgccgaag cggccggcat tcctgcgctc acgcccgaac
     9001 ccatcgcgga caatccttgg ttcctcgcgg cgaccaaccg cgtggattac ctgatcattt
     9061 cttcgcgcgc gctggccgcc gccgcgcaag agctggccga ttatcgcgct ggacagggcc
     9121 tgcgcgtggg cctcgccact tttgaggacg cgtgcgattg gttcgccggc ggcctgcgca
     9181 cgccggaagc catccccgcc ctgttgcgcc acgcgcacac tgtttgggcg gaagcgccgt
     9241 ggctggtcgc gttggcgggc aacggccact acgattatct caacgcgctg aatctggaag
     9301 ccaaccacgt gccgccgctg ctgaccgcgt cctacgaggg cctcttcgcc gccgacggcc
     9361 tcttggccga ttggaccggc gacggccaac ccgatgtggc cctcggccgc ctgcccgcgc
     9421 gcacgcccgc cgagctggcc gccatgatcg ccaagatcaa ggcctacgag caggacttcg
     9481 gccacgcttg ggaaaatcaa atcgtgcttg ccgccgatgc cgcggatgcc gcgggcgact
     9541 tcaccgccgt caacgcgcaa ctggcagcgc tggccggcgc ggaatacccc gtggccacgg
     9601 tggaccttgc cgcgcagggc ttggcgccgg cgcgcgccgc gctgctcaac tggttcggca
     9661 gcggcgcagg catcatccac tacaccgggc acggcaacgt caattcgtgg agcggcaagg
     9721 gactgctgaa ggcggcggac gtcaacgcgc tgaacaacgc gcgccagccg gtggtggtgg
     9781 cgttgagctg tctgatcggc cgctacgaag cgcccggcgt gcagagcctc ggcgagctgc
     9841 tcatgcagcg ggccgccggc ggcgcagtcg ccgtgtgggg cccttcgggc ctctcgcgca
     9901 accatccggc ggcagaactc ggcgcggcgt tctatgacct cgtgttgcag cagggcgccg
     9961 gtacgctggg cctggccgtg cagcaggcgc gcgccgcgct gcccgccgaa accttcaccc
    10021 gcgacacctt cgccatctat aatctgctgg gcgaccccgc actccggatg gccggcaaca
    10081 ctggcgctgg ccgcaccgcc gccagctttg cccagtggcg ctgggcgcag tatgcgccgc
    10141 acgagctgaa cgaccccgcc accagcgggc ccaccacgca caacttccag ctctacgcca
    10201 tggatggcgc gggcgagctc gtggccgaac tgccggaatt cggctacgcc atccctgacg
    10261 aagcggccgg cggcggcttc atcatgcgct ggcagcggcg cgtcaaccgc ccagacctcg
    10321 actaccactt ctatttctcg cccgacctcg tcacgtggag caacatcacc accggcacgg
    10381 agaccctcag cgtggaaccc gccccggacg gcatcatgga aaccgtccgc acccgcgtga
    10441 acctgcccgc cgccgaaaat attttcctcg gcgtgaaggc cgtgcgcaaa taa
//
LOCUS       DAOPNP010000089        10471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_7228_length_10471_cov_9.947389, whole genome shotgun sequence.
ACCESSION   DAOPNP010000089 DAOPNP010000000
VERSION     DAOPNP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_10471_cov_9.947389"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1413)
                     /locus_tag="PKI64_08240"
     CDS             complement(97..1413)
                     /locus_tag="PKI64_08240"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOE01034.1"
                     /translation="MTPATYNLWTIGCQMNEADALRAATLLEAAGYRPVPHAQEASLL
                     LLNTCVVRQQAEDKIYSRLTFVTELKKRHPDLVVVLMGCLVGKNPLEIQRLQERLPYV
                     DVFLPPSDVTPLREFLQARDMALPADEPDEDLPPPLPPSLAGRAIVAHVPAVLGCSHA
                     CTYCVIPYRRGGERSRPAAEILAEVADLAARGVREVILLGQIVDRYGLDRPGEIRLPE
                     LLRRVAAVPGILRVRFLTSHPQWITSELIATVADTPGLCPYFEIPVQAGSDAVLAAMR
                     RGYTVAEYEALIARIRAAIPHAGLSTDVIVGFPGETAAQFEESMALMRRINPDMIRIA
                     KYSPRPLTFSARHLPDDVPAEEKERRRSAMEAQLRVMLTEKNVPLRGQTVEILVESIE
                     ASGRRYGRTPDYKPVFVDHGTARPGELIRARIVWAGPFSLIATAAE"
     gene            complement(1431..1937)
                     /locus_tag="PKI64_08245"
     CDS             complement(1431..1937)
                     /locus_tag="PKI64_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01035.1"
                     /translation="MRDDDEFDPYRRFESGCDIHGDNYLRECSVCGAEFCFACFSQSA
                     LCADCAAQGEMEDDEEAADNASEPELALLDGFDDDEPPADAPSLPPPPPAKPATPVKK
                     PKGQAKPTAKAKPKAKAKPAIKPTRKAPPKTKTKPKAKAKPKATRKIAKPAKAKAKAK
                     AKRAARRA"
     gene            2134..3318
                     /locus_tag="PKI64_08250"
     CDS             2134..3318
                     /locus_tag="PKI64_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE01036.1"
                     /translation="MKSAFWNKLIERLDKLDSGSVQAQFLRLASERGLLETIFHALRE
                     GILMLDAEGRVLYANRGACRLLGMDETTATGQPMKRYIREIEWDRLLRLDETEWARLA
                     NREIEITHPEHRFLAMYLAPLENPTDGRPGVVMILRDVTQERHRAAEVVESERLNAIL
                     MLAAGVAHEIGNPLNSLAIHLQLMDRDLRKLPAEQVANLWESLDVARGEIQRLDHILA
                     QFLKAIRPSLPNFARHSLPELVAATLQTLDAEIHDRKVVVDVDAPTDLPSAWVDEGQV
                     RQAFFNVIRNALQAMGGGGVLRISFVTLDRLVGVVFQDSGPGIPPEQMTQLFEGFRST
                     KRTGHGMGLMIVQRIVRDHGGTVLVDSLPAGTRIQLNFPRDDQRIRLLAAPAEPAPEA
                     VP"
     gene            3315..4682
                     /locus_tag="PKI64_08255"
     CDS             3315..4682
                     /locus_tag="PKI64_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE01037.1"
                     /translation="MSQRPTILIVDDERHTREGLARALRGDYAVAEAENGQRALEWLE
                     THAADVILTDLRMPHMDGLQLLARLLGREPKPIVIMLTAYGNVETAVEAMKRGAYDFL
                     AKPVNLDRLELLLTRALAERQLGVENQRLRAQLDSKYGFANIIGTSPAMQAVFDTIRH
                     VAPTKATVLIQGESGTGKELVARALHQASPRREGPFVPVHCAALAPTLLESELFGHEK
                     GAFTGAVERRRGRFEMADGGTLFLDEIGEIEPSLQVKILRVLEERAFERVGGSETVTV
                     NVRLVAATNKDLRQLVADGKFREDLFYRLFVVNLTLPPLRERDGDIVLLTQHYLKTLA
                     AENGKSVPTLTAEAMDALTAYSWPGNVRELRNVIERMVVLNSTGKLNLDDVPATVRQG
                     AHDSGRDATRAGRVLRDAERQLIEDALRQHRGNRTKAAQHLGISRRTLHRKLNEFGLR
                     SRSND"
     gene            4698..6422
                     /locus_tag="PKI64_08260"
     CDS             4698..6422
                     /locus_tag="PKI64_08260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOE01038.1"
                     /translation="MALAFESKVQNLIFQMDQGSGRKVIHAVLFVLFACAVAALYTFT
                     NFQGLNDEGAFDSAQLALNHAEQGELMTRCVRPLSIWKVAAHSPTDDAAVMHHPDLLH
                     PPLWPLMLSGVFRLTGIPTAGQPTTAYVNGWDYVPVVASHVFTALATLLVWLIGRKLF
                     DHRVGMLAAISFLVCDGIWRQSLLGAEWSAALFFVLGAFYAAILAADLPPGIGPQDDQ
                     GAAWRWLIPLMISSVFTTAAFLTRYAAGFAAVAIFLFMGASRRSRPWSKAGLYLVLAA
                     VPVIPWVVRNIKVSGSAFGLALYNLLSDTYLFPGDALARSVTPTMPDALTAIYAVQLK
                     AMANLREFAGQGFGLAGVGILLALFGAMYFHRFVRPATRTLRWSLLPALVLTILSAAA
                     FGADSLRAMVFYWPLVMVFGWAFLLVLLDRRQFELRFLPPAVLTLVMFLSAFPLLLQV
                     LPPRTGLPYPPYFHRYIGWTVAMVEPDECLTTDIPWATAWYGNRTSILLPRTLDGFYE
                     IHNQYQPLAMAYFTTVTRDKPWVRGLSDPNAPEYSWYQIFSAGKVPANFPLTNGRFLA
                     GTDQMILR"
     gene            complement(6446..7636)
                     /locus_tag="PKI64_08265"
     CDS             complement(6446..7636)
                     /locus_tag="PKI64_08265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE01039.1"
                     /translation="MAAPDRLAFVCPRLAGSGAVGGAETLLKNLATHAAARGVAVDFL
                     TTCAQSHFTWENTLPPGVERMGGMDVHFFPVDERNPGQFLQIQQQIDRGRPVSVEEEL
                     LWLKNGVNSTALMQHLAQHAASYRAILLGPYLFGLTWFAALAHPERALLVPCLHDEPF
                     AKLACMRRLFAETAGCLFNTQAERELAGELFQYPDARARIVGMGLDAFTADPTAFAKR
                     RKINAPYVLYSGRREPLKGTPLLCDYLHAFRQRTGRDVKLVLTGSGDIDAPAELWPHI
                     FDAGFVSEDEKHEAMAGAVAFVHPSVNESFGIVLLEAFLAGTPGLVHARSRVLVSQCR
                     AANAGLWFRHYPDFETQLTYLLDHPAERAQLGRNGRDYVTREYAWPVIEDKFFAALDA
                     LLPR"
     gene            complement(7623..8081)
                     /locus_tag="PKI64_08270"
     CDS             complement(7623..8081)
                     /locus_tag="PKI64_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01040.1"
                     /translation="MSDTPILSIGAEGLDVAQLVANLQATVERKARDGVYADARVARA
                     EKTNLIHLRGTDDFITFYLASLREAVFVDIADFEIRERRRVFGRLLVAFKTLIWKLLK
                     FYTYRLWSQQNQVNGLIITALEGMEEQNAARVAALEKRIAELEKAGHGRA"
     gene            complement(8142..8627)
                     /locus_tag="PKI64_08275"
     CDS             complement(8142..8627)
                     /locus_tag="PKI64_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOE01041.1"
                     /translation="MYLRKVISGGQTGADQGALSACWRLGVPHGGWVPKGRRTEKGKV
                     PARYRMRQHWSRHYPPRTEKNIVDSDGTLIFTFGALDGGSLLTKQLARKHRRPCLHVD
                     LRQPLPANVRRIERWIHRRLPRGGVLNVAGSRRSKAPGIHLAVRRAMTAVLRRLAASP
                     A"
     gene            8881..10224
                     /locus_tag="PKI64_08280"
     CDS             8881..10224
                     /locus_tag="PKI64_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOE01042.1"
                     /translation="MKQNETQWIERLSAMAAASCQLDSELYVKYDVKRGLRDINGKGV
                     RAGLTQIGDVQSHPEGDETGPGHLIYRGIDIDDLVAGFLTDGRPGFEETTYLLLAGEL
                     PNREELNGFCELLGSYRGLPDSFVHDSLLKLTSRDMMNAMAQSILAMYILDDQAEDVS
                     LANVLRQCLKLIAVFPLLAAYSYQAYTYFYGKGSLVIHSPQPELSTAANFLHMLRNDS
                     AYTPLEAQLLDLALVLHAEHGGGNNSSFTTHVVTSSLTDTYSTIAAAMGSLKGPRHGG
                     ANAKVVAMFEDMKQEVKDWTNDNQISDYLHKLLKKEAFDRAGLIYGIGHAVYSTSDPR
                     TIILREQVEKLAAEKGLAAEMDLYRRVERLAPEIISTQRKMYKGVSANVDFYSGFLYR
                     LLEIPAELFTPIFAISRIAGWSAHRIEELANGGKIIRPAYKSVAPRRDYQPLAAR"
BASE COUNT         1948 a         3109 c         3395 g         2019 t
ORIGIN      
        1 gctagagcac aggctttcgt cgggtgggaa tggctggtcg gtctgcgacc gttccagccc
       61 tgtaatccag atttctgcaa atgcgcgaaa aacaaatcac tccgccgcgg tggcgatcag
      121 tgaaaacggc ccggcccaca caatgcgggc gcggatcagt tcgccggggc gcgcggtgcc
      181 atggtccaca aacaccggct tgtaatccgg cgtgcggccg taccgacgac cggaagcctc
      241 gatgctttcc accaggattt ccactgtttg gccgcgcagg gggacgttct tttccgtcag
      301 catcacgcgc agttgtgctt ccatggcgct gcggcggcgt tctttttcct cggcagggac
      361 atcatcgggc aaatgccgcg cggaaaaggt cagggggcgc ggcgaatact tggcaatgcg
      421 gatcatgtcg gggttgatgc gccgcatgag cgccatgctc tcctcgaatt gcgccgcggt
      481 ttcgccggga aaaccgacaa tgacatcggt ggatagtcca gcgtgcggaa tggccgcgcg
      541 gatgcgcgca atcagcgcct catattccgc gacggtgtag ccccggcgca tggcggccag
      601 cacggcatcg gaaccggcct gcaccggaat ctcaaagtag gggcagagac ccggggtatc
      661 ggcgacggtg gcaatgagtt ccgacgtgat ccactgcgga tgactggtca gaaagcgcac
      721 gcgcagaata cccggcacgg ctgccacgcg gcgcagcagc tccggcagcc ggatttcacc
      781 ggggcggtcc agaccgtagc gatccacaat ttgcccgagt aaaataacct cgcgcacgcc
      841 gcgcgcggcg agatcggcca cttccgccaa aatctcggcg gcgggacggg agcgctcgcc
      901 accacgacgg taaggaatca cgcagtaggt gcaggcgtgc gagcacccca gcacggccgg
      961 cacgtgggcc acgatggcgc ggccggccaa cgaaggcgga agcggcgggg gcaaatcctc
     1021 atcgggttca tccgccggca gcgccatgtc ccgcgcttgt aaaaattcgc gtagcggagt
     1081 gacgtccgac ggcggcagaa acacgtccac gtagggcagc cgttcctgca gccgctgaat
     1141 ctccagtggg tttttgccca cgaggcaacc catcagcacg accaccaaat caggatggcg
     1201 ttttttaagt tcggtgacga acgtcaggcg cgagtagatt ttatcttccg cctgctgccg
     1261 caccacgcac gtattgagca acaggagcga ggcttcctgg gcatgtggca cagggcggta
     1321 gcccgcggct tcaagcaggg tggccgcgcg cagggcatcg gcctcgttca tctggcagcc
     1381 gatggtccac aaattgtacg tggccggcgt caacaggctg actcaaccgc tcaggcgcgc
     1441 cgtgcggccc gtttagcttt ggccttggcc ttggctttgg ccggcttggc gattttgcgt
     1501 gtggccttgg gcttggcttt ggccttgggc ttggttttcg ttttgggcgg ggctttacgc
     1561 gtcggcttga tggcgggctt ggccttggct ttgggtttag ccttggcagt cggcttggct
     1621 tgacccttgg gcttctttac gggtgtcgcc ggtttggcag gagggggagg ggggagagag
     1681 ggggcgtcgg cgggaggttc gtcatcatcg aatccatcca gcagggcaag ttccggctcg
     1741 ctggcgttgt ccgcagcctc ttcgtcatcc tccatctcgc cttgcgcggc gcaatcggcg
     1801 cacaacgcgg actgggagaa gcaggcaaaa cagaactcag cgccgcagac gctgcattcg
     1861 cgcaggtagt tgtccccgtg aatgtcgcac ccgctttcaa agcggcgata aggatcaaat
     1921 tcgtcatcgt ctctcatggt ccacacctat ccaaaaatga tttctgtcgc gcgtcctgtc
     1981 gcatgatcca tcatacaacc atatcctact gttcgcacga gcttcgcata gcgtcaagtg
     2041 gcgaattgca ggaaaatttc acccgcgatt ttttgtttgc ggcgcccgcg ggcattgtgg
     2101 agagtaagga actgcggtga aacgcggcaa gacatgaagt cggcattctg gaacaaattg
     2161 atcgaacggc tggacaagct ggactcgggc agtgtgcagg cgcaatttct ccgcttggca
     2221 tcggaacggg ggctgctgga aaccatcttc cacgcgttgc gcgaagggat cctgatgctg
     2281 gatgccgaag gccgcgtact ctatgccaac cgcggcgcgt gccgcctgct ggggatggat
     2341 gaaaccaccg ccaccggcca acccatgaag cgctacatcc gcgaaatcga atgggaccgg
     2401 ctgttgcggc tggatgaaac ggaatgggcg cgcctggcca accgggaaat tgaaatcacg
     2461 catcccgagc accgttttct ggccatgtat ttggcaccct tggaaaatcc cacggatggc
     2521 cggccaggcg tggtgatgat tctgcgcgat gtgacccagg aacgccaccg cgccgcggaa
     2581 gtggtggaat ccgagcgatt gaatgccatc ctgatgctgg ccgcgggcgt agcgcacgaa
     2641 atcggcaacc cgctcaactc gttggccatt catttgcaac taatggaccg cgatctgcgc
     2701 aagctgccag cagaacaggt ggccaatttg tgggaatcac tggatgtggc gcgtggcgaa
     2761 atccagcggc tggatcatat tctggcgcag ttcctcaagg ccatccggcc cagcttgccg
     2821 aactttgccc gccactcgtt gccggagttg gtggcggcga cgttgcaaac gctggatgcc
     2881 gaaatccatg accgcaaggt ggtggtggat gtggacgcgc caaccgactt gccatcggcg
     2941 tgggtggatg agggacaggt gcggcaggca tttttcaacg tgattcgcaa tgcgttgcag
     3001 gcgatgggcg ggggcggggt tttgcggatt tcgttcgtga cgctggaccg gctggtaggc
     3061 gtggtctttc aggactcagg cccggggatt ccgcccgaac aaatgacgca actatttgaa
     3121 ggctttcgca gcaccaagcg caccggccac ggcatggggc tgatgattgt gcagcggatt
     3181 gtgcgcgacc atggcggcac ggtattggtg gacagcctgc ccgcgggcac gcgcatccag
     3241 ttgaacttcc cgcgcgatga ccagcgcatc cgcctgctgg ccgcgccggc ggaaccggcc
     3301 ccggaggccg tgccatgagc caacggccaa ccattttgat tgtggatgat gagcgccaca
     3361 cccgcgaagg attggcccgc gccctgcgcg gcgactacgc cgtggccgaa gccgaaaacg
     3421 gccagcgggc cttggagtgg ctggagaccc acgccgccga tgtgatcttg accgatctgc
     3481 gaatgccgca catggacggc ctgcaactgc tggcccggtt gctgggacgc gagcccaagc
     3541 ccatcgtcat catgctgacc gcgtacggca atgtggaaac cgccgtggag gcgatgaagc
     3601 gcggcgcgta cgattttttg gccaagccgg tcaacttgga ccggctggag ctgctgctga
     3661 cccgggcgtt ggccgaacgg caactgggtg tggaaaatca gcgcctgcgc gcgcaattgg
     3721 attccaagta tgggtttgcc aacatcatcg gcacatcgcc ggccatgcag gcggtgttcg
     3781 acaccatccg ccatgtggcg cccaccaagg cgacggtact gattcagggc gaaagcggca
     3841 ccggcaagga actggtggcg cgcgcgctgc accaggcaag cccgcgacgc gaaggtccct
     3901 ttgttcctgt ccattgcgcg gcgctggccc cgaccttgct cgaaagcgaa ttattcggcc
     3961 acgaaaaagg cgctttcacg ggcgcggtgg aacggcggcg ggggcgcttc gagatggccg
     4021 atggcggcac gctgtttctg gatgaaatcg gcgaaatcga gccgtcgctt caagtcaaaa
     4081 tcctgcgggt gctggaagaa cgcgcgtttg agcgggtggg cggctcggag accgtgacgg
     4141 tgaatgtgcg tttggtggcg gccaccaaca aagacctgcg gcaactggtg gccgacggaa
     4201 aattccggga agacctgttt taccggcttt ttgtcgtgaa tctcacgttg ccccccctgc
     4261 gcgaacgcga tggcgacatt gtgctgctga cccagcatta tctgaaaacg ctggcggccg
     4321 aaaacggcaa gtccgtgccg acactgactg cggaagccat ggatgcgctg acggcgtatt
     4381 cgtggcccgg caacgtacgc gagttgcgca acgtaatcga gcgcatggtg gtgctgaaca
     4441 gcactggcaa actgaatctt gatgatgtgc ccgccaccgt gcggcaggga gcccatgatt
     4501 ccgggcgcga cgccacccgt gccgggcggg tgctgcgcga cgcggaacgg cagctcatcg
     4561 aagacgcgct gcgccagcac cgcggcaacc gcaccaaggc cgcgcagcac ctcggcatca
     4621 gccgccgcac tttgcatcgc aaactcaacg aatttggttt acgctcccgg agtaatgact
     4681 aacccaaggc ggaccccatg gcgcttgctt ttgaatccaa ggtacaaaac ctgattttcc
     4741 agatggatca gggctccggc cgcaaggtga tccatgccgt gctgttcgtg ctgtttgcct
     4801 gcgccgtggc ggcgctctac acgttcacca actttcaagg actgaatgac gaaggcgcgt
     4861 ttgactctgc ccagcttgcg ctcaaccatg cagagcaagg ggagctgatg acccgctgcg
     4921 tccggccgct gtccatttgg aaagtggcgg cgcattcgcc gaccgatgat gccgcggtga
     4981 tgcaccaccc tgatttattg cacccgccgc tgtggccgct catgctctcc ggcgttttcc
     5041 ggctgacggg catccccacc gccgggcaac ccaccacggc ttacgtcaac gggtgggact
     5101 atgtgccggt ggtggccagc cacgtcttta ccgcgctggc aaccctgctg gtgtggttga
     5161 ttggccgcaa gctgtttgat caccgggtgg gcatgctggc ggccatttcg tttttggtct
     5221 gcgacggcat ttggcggcag agcctgctcg gcgccgaatg gtccgccgcg ctcttttttg
     5281 tgctgggcgc gttttatgcc gcaatcctgg cggcggattt gccgccgggc atcgggccgc
     5341 aggatgacca aggggcggcg tggcgctggc tgatcccgct gatgatcagt tcggtcttca
     5401 ctaccgcagc cttcctgact cgctacgcag ccggatttgc ggcggtggcc attttcctgt
     5461 ttatgggcgc gtcgcggcgg tcgcggccct ggtccaaggc cggcttgtat cttgtgctgg
     5521 cggcggtgcc cgtgattccg tgggtggtgc gcaacatcaa ggtgagcggc agtgcgtttg
     5581 gcttggcgct ctataatctg ctgagcgaca cctacctgtt cccgggggat gcactggcgc
     5641 gcagcgtcac gcccaccatg ccggatgcgt tgaccgccat ctatgccgtg caactgaaag
     5701 ccatggccaa cctgcgcgag tttgccgggc aggggttcgg tttggctggc gtcggtattt
     5761 tgctggcgct gtttggcgcg atgtatttcc accgtttcgt gcgtccggcc acccgcacgt
     5821 tgcgttggag cctgctgccg gcgctggtgt tgaccattct ctccgccgcc gcattcggcg
     5881 cggatagcct gcgcgccatg gtgttctact ggccactggt catggtgttc ggctgggcct
     5941 ttctgctggt gctgctggac cgccgtcagt ttgaactgcg cttcctgccg cccgcggtct
     6001 tgacgttggt gatgtttttg agcgccttcc cgctgctgct gcaagtgctg ccgccacgca
     6061 caggcctgcc ctatccgccg tatttccacc gctacatcgg ctggaccgtc gccatggtgg
     6121 agccggatga atgcctcacg acggacatcc cctgggccac ggcgtggtac ggcaaccgca
     6181 cctccatttt gctgccgcgg acgttggacg gattttacga aatccacaac cagtaccaac
     6241 cgctggccat ggcctatttc accacggtca cgcgcgacaa accgtgggtg cgcggtcttt
     6301 ccgatccgaa tgcgccggag tattcgtggt atcaaatctt ttccgcgggc aaagtgccgg
     6361 ccaacttccc gctgaccaac ggccgctttt tggccgggac cgatcaaatg atcctgcgct
     6421 gacgccgccg cgcggcgggg cagggtcagc ggggcaacag cgcgtcgagc gcggcgaaaa
     6481 acttgtcttc aatgactggc cacgcgtact cgcgggtgac atagtcgcgg ccgttgcggc
     6541 ccagttgggc gcgttctgcc ggatggtcca gcaagtaggt cagttgcgtt tcaaagtcgg
     6601 gatagtgccg gaaccagagc ccggcattgg cggcacgaca ttggctgacc agcactcggc
     6661 tgcgggcgtg gaccagtccc ggcgtgccag ccaaaaaagc ctccagcagc acgataccga
     6721 aactttcatt caccgacgga tgcacaaacg caaccgcgcc cgccatggcc tcgtgttttt
     6781 catcttcact gacgaagccc gcatcgaaaa tgtgcggcca gagctccgcc ggcgcatcaa
     6841 tatcaccgga gccggtcagg accagcttta cgtcgcgccc ggtgcgctgg cggaaggcgt
     6901 gcagataatc gcagagcagc ggggtgccct tgagcggctc gcggcggccc gaatagagga
     6961 cgtaaggcgc gttgattttg cggcgtttgg cgaaggcggt ggggtcggcg gtgaacgcat
     7021 ccagccccat gcccacgatg cgggccctgg catcaggata ctggaacagt tcgccagcca
     7081 actcgcgttc ggcctgcgta ttgaaaaggc agccggcggt ttccgcgaac aggcggcgca
     7141 tgcaggccag tttggcaaag ggttcatcgt gcaggcaggg caccagcagc gcgcgctcgg
     7201 ggtgagccag cgcggcgaac caagtcagcc caaagaggta gggccccagc aaaatcgcgc
     7261 ggtagctcgc cgcgtgttgc gccaagtgct gcatgagcgc agtcgaattg accccgtttt
     7321 tcaaccagag caattcctcc tcgacggaca ccggccggcc gcggtcaatc tgctgctgga
     7381 tttgcaggaa ctggccggga ttgcgttcat ccaccgggaa aaaatgcaca tccatcccgc
     7441 ccatgcgctc cacgccgggc ggcaacgtgt tttcccaagt gaagtggctc tgggcgcaag
     7501 tggtcaggaa atccacggcc acgccgcgcg ccgcggcatg agttgccaga ttcttgagga
     7561 gcgtttccgc gccgccgacg gcgccggagc ccgcgaggcg cgggcagaca aacgccagcc
     7621 ggtcaggcgc ggccatggcc cgccttttcc agttctgcaa tgcgcttttc cagcgcggcc
     7681 acgcgggcgg cgttttgttc ttccatgccc tccagcgcag tgatgatcag cccgttgact
     7741 tggttctgtt gcgaccagag ccgataggtg taaaacttca gcaacttcca gatcagggtt
     7801 ttgaaggcca ccagcaggcg gccgaaaacg cgccggcgtt cgcggatttc aaagtcggcg
     7861 atgtccacaa ataccgcctc gcgcaggctg gcgagataaa aggtgatgaa gtcatcggtg
     7921 ccgcgcaagt ggatcaggtt ggttttttcg gcgcgggcca cgcgggcatc ggcatagacg
     7981 ccgtcgcgcg ctttgcgctc gaccgtggct tggaggttgg ccaccagttg ggccacatcc
     8041 agcccttcgg cgccaatgga taagattggg gtatcgctca tgggggcgaa tgtaaagcgc
     8101 ggggaggggg gggtgtcaag gcgcgaggtg cgcgggttgg ctcaggcggg cgacgccgcc
     8161 agccgccgca gcacggccgt catggcgcgg cgcacggcca gatgaatgcc gggcgccttg
     8221 ctgcggcgcg agcccgccac gttgagcacg ccgccacgtg gcaggcgacg atggatccaa
     8281 cgctcgatgc ggcgcacgtt ggccggcagc ggttgccgca aatccacatg gaggcagggg
     8341 cggcggtgtt ttcgggccag ttgcttggtc agcagcgagc cgccatccag cgcgccaaac
     8401 gtgaaaatga gcgtaccgtc ggaatccacg atgttttttt cggtgcgcgg cggataatgg
     8461 cgcgaccaat gctggcgcat ccggtagcgt gcgggaactt tgcccttttc ggtgcgacgg
     8521 cccttgggta cccagccgcc atgcggcacg ccgagtcgcc agcacgcgct caacgcacct
     8581 tgatcggcgc cggtttgccc gccggagatg actttacgca aatacattta tattttttta
     8641 tcacgcgcgc tggcgcggca cgagcgtttt cccggttgcg cggcgtgagg gatggtcgtc
     8701 gcgcgcaacg atgcaaaaaa gagaaactgt gttttatgcg ctttgatttc ggggcggatg
     8761 cggtatactg cttgcctcca tagatttgac agggagcgct tccccggttc gcatgccaca
     8821 gggccgccac ggaagcgcac ttgccgcgcg agggcggggc gcaggcagaa aggacaagac
     8881 atgaaacaaa atgaaaccca atggattgaa cggttgagcg ccatggccgc ggccagttgc
     8941 cagttggatt ccgaattgta tgtgaagtat gacgtgaagc gcgggttgcg cgacatcaac
     9001 ggcaagggtg tgcgtgcggg gctgacgcag atcggcgacg tgcagtcgca tcccgaaggc
     9061 gatgaaaccg ggccgggcca tctgatttat cgcggaattg atattgatga cctcgtggcc
     9121 ggttttttga cggatggccg cccgggattt gaagagacga cctacctgct gctggcgggc
     9181 gaattgccaa accgtgagga actgaatggt ttttgcgagc tgctcggctc ctaccgggga
     9241 ttaccggata gttttgtgca tgattcgctg ctaaaactca ccagccgcga catgatgaac
     9301 gccatggctc aaagcattct ggccatgtat atcctcgatg accaagcgga ggatgtgtcg
     9361 ctggcgaacg tgctgcggca atgcttgaag ctgattgcgg tgttcccgct gctggcggcc
     9421 tacagctatc aggcctacac ctatttttac ggcaagggca gcttggtcat tcacagtccg
     9481 caaccggaac tgtccaccgc ggccaacttc ctgcatatgc tgcgcaacga cagcgcctac
     9541 acgccgctgg aagcgcagtt gctggatttg gcgctggtgc tgcacgcgga gcatggtggc
     9601 ggcaacaatt cgtcgttcac cacccacgtg gtcacatcga gcctcaccga cacctattca
     9661 accatcgcgg cggcgatggg atcgctgaag gggccgcggc acggcggggc caacgccaaa
     9721 gtggtggcaa tgttcgagga catgaaacag gaagtcaaag actggaccaa tgacaaccag
     9781 atcagcgact acctccacaa gctgttgaag aaggaagcct ttgatcgggc ggggttgatc
     9841 tacggcatcg gtcacgcggt ctattccacc tccgacccgc gcacgatcat cttgcgcgag
     9901 caggtggaaa aactcgcggc ggagaaaggc ctcgccgcgg aaatggactt gtaccgccgg
     9961 gtggaacggc tggcgccgga aatcatcagc acgcagcgca agatgtacaa gggcgtcagc
    10021 gccaacgtag atttttattc gggctttttg tatcgcctgc tggaaatccc cgcggaactc
    10081 tttacgccga tattcgccat ctcacgcatt gcgggctgga gcgcgcaccg gatcgaagaa
    10141 ctggccaatg gcggcaaaat tatccgcccc gcctacaaga gcgtggcccc gcggcgcgat
    10201 taccagccgc tcgcagcgcg ctaacgcatt gtcgggcagg aacggctgga aggtctgcga
    10261 ccgttccagc ccttcaatcc gaccggctgc ttttgtaggg cgggaatggt tgaggaacga
    10321 taccttcccg ccgggaacgg ctggaaggtc tacgaccgtt ccagcccttc aatccgaccg
    10381 gctgcttttg tagggcggga atggttgagg aacgatacct tcccgccggg aaaggctgga
    10441 aggtctgcga ccgttccagc ccttctatgg g
//
LOCUS       DAOPNP010000090        10334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_7365_length_10334_cov_9.548302, whole genome shotgun sequence.
ACCESSION   DAOPNP010000090 DAOPNP010000000
VERSION     DAOPNP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7365_length_10334_cov_9.548302"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..500
                     /locus_tag="PKI64_08285"
     CDS             63..500
                     /locus_tag="PKI64_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE01043.1"
                     /translation="MKISTKGRYGLRTLMDIALHQGKGPVNLNDIAQRQGISAKYLWQ
                     IVNLLKTAGFVRGTRGPKGGYVLLRPTADITLLDVLQTLEGPFSLVECVDDPEVCQRS
                     GVCVAHSIWEEVSQAIRAALQKITLAEILRRHHSADSAHHFVI"
     gene            complement(514..1623)
                     /gene="hemW"
                     /locus_tag="PKI64_08290"
     CDS             complement(514..1623)
                     /gene="hemW"
                     /locus_tag="PKI64_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOE01044.1"
                     /translation="MNHPAGMSPGLYIHFPFCVRKCPYCAFFSETPRSATVAAWREGL
                     VRELESLPRGFVPRSIFFGGGTPTALSPDDVASVLDLVRAHVPLTQIEEWTCEVNPGT
                     LTRPMAELLRQAGVNRLSIGAQSFTDPTLQKLGRIHQSVETRECVAMARAAGFDNIGL
                     DLMYGIPGVAADDFMADVEAALELGPEHISGYALEIEEGTPWAREAAAGRLAVVEDDQ
                     RAQFDWLRQRLPAAGLVHYELSNFARPGHECRQNLLYWSGGEYIGVGPAAHSHWQGAR
                     WGNTNTLPNWQREFTEQLEPAAKARETLVMGLRRLAGWGREEFRAATGFDYDDLRGAE
                     IARLGREGLLVVTPERIRLADDALFISDTVFAELV"
     gene            complement(1620..1850)
                     /locus_tag="PKI64_08295"
     CDS             complement(1620..1850)
                     /locus_tag="PKI64_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOE01045.1"
                     /translation="MCLAIPGKIVAIREGEGFDRRGTVDYDGVRQEVALAYLPEAQIG
                     DYVLVHVGFAMTRLDTAEAERILRELHELENP"
     gene            complement(1855..2277)
                     /locus_tag="PKI64_08300"
     CDS             complement(1855..2277)
                     /locus_tag="PKI64_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01046.1"
                     /translation="MPMTPDLQCTLLCDDVRQERSGKFILIGLFDQLGSPSFPFRHPR
                     MFLVTRWCSGEGEFRQHTRILRPDMSTVMIEGRDIPVKLPTAEATATNVELFINTEFH
                     EAGIHWVEVLLDNDIKMRFPLRVTKMTQPPPGMAEGGV"
     gene            complement(2401..3816)
                     /gene="pyk"
                     /locus_tag="PKI64_08305"
     CDS             complement(2401..3816)
                     /gene="pyk"
                     /locus_tag="PKI64_08305"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOE01047.1"
                     /translation="MRKTKIVCTIGPASESPEVLGRMMDAGMNVARLNFSHGTHADHA
                     AKIALLRQIADEKGLPIAILQDLAGPKIRTGAFACGSIQLQTGAEFVLTARDVPGDEH
                     EVGLTYPELPQDVQVGDILLLADGALELRVEKVEGPDIHCRVILGGRLSSHKGINLPS
                     RSIRAPILTKKDYDDLAFGLAQGVDFVALSFVRSAADIEVARAYMRAQGRIRPIIAKI
                     EKHEALQDLDTIIAAVDGVMVARGDLGVDIPPEKVPTAQRMIIEKANFAGKPVITATQ
                     MLKSMTDAPRPTRAEVTDVTNAVLEGTDAVMLSEESAMGNYPVEAVATMARIVVEAEK
                     GFLRAAWWQRLQQVQTADESEALTRAACSLAETIEAKALVIATQTGGTAKLAAKTRPV
                     QPILAGTTEVDAFRCLSLVWGVRPMMIQRVDLLIDLFAEMHRAAKATGVVRPGDSVVC
                     TAGFPLGKTGASNLIKVEIVP"
     gene            complement(3859..4551)
                     /locus_tag="PKI64_08310"
     CDS             complement(3859..4551)
                     /locus_tag="PKI64_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOE01048.1"
                     /translation="MKTWRRKKVVLFDIDGTLLDTHGHGREAFIRGLAKLTGERDELE
                     YIAFAGNTDRNVLDQVMARRGLKFDESEIEQVFRHVAEELRGLLAKHPAREINGARRL
                     LSALAESGAQLGLVTGNIRDCAFLKLGSVGLADFFSFGGFGDHHADRAEIARAALGEM
                     KQMGVVAELDEVCLVGDTPYDVQAGVALGIPVLGVATGRHDVGILREAGAALAVADYG
                     DVPGLLGWLGHG"
     gene            4724..6667
                     /gene="ftsH"
                     /locus_tag="PKI64_08315"
     CDS             4724..6667
                     /gene="ftsH"
                     /locus_tag="PKI64_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE01049.1"
                     /translation="MDPKQNNPKDPQGQRRPPMPMRALVAWFLLIALVVTVFQLATQH
                     SNKATSLPYNPDFLTLLKEGRINKCEVVMEVSGQSFIRGELKELDGTGIKQFRVETPV
                     TENLVEKLTAAKVPFEFKPQNPLVWQIVSSTVPFLLVFALIYFLFYRNMSGSARGAFN
                     FGKSRARLMTRDTNKITFKDVAGADEAREEVQEIIEFLRDPKKFQRLGGRIPKGVLLV
                     GPPGTGKTLIAKAIAGEAQVPFFSISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIF
                     IDEIDAVGRSRFSGIGGGHDEREQTLNALLVEMDGFDTQEGVIIIAATNRPDVLDPAL
                     LRPGRFDRQIVVDLPNLTGREAILKIHARKVKLDATVQLRNIARGTPGFSGADLANLM
                     NEAALLAARRGAETIHEKDLEEARDKVRWGRERRSRVLDDKEKRLTACHEAGHAIVLA
                     LTPGAEPLHKVTIIPRGQALGATFQLPEKDRYTEGRERLLGMITGTMGGRVAEQLCFG
                     EITTGAASDIKQATHIARMMVTEWGMSDLLGFQHFGTREELLFLGREVSRTNEMSEET
                     ARKIDAEVKRIIDECYQKAVDILTHNRAKLDLLTSLLIERETLDGRDVEEILKHDRIL
                     TAAERNAEDAVKEAETPAAPAAE"
     gene            6888..7613
                     /gene="folP"
                     /locus_tag="PKI64_08320"
     CDS             6888..7613
                     /gene="folP"
                     /locus_tag="PKI64_08320"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOE01050.1"
                     /translation="MNLTPDSFTDGGQFLDPAAAEEQAYKLAADGAHILDIGAESTRP
                     GHELIPAAEELRRLLPVLDRLAGKLRIPISIDTSKPEVARAAFDRGATILNDVAALTR
                     PGMLDFARAGHFPVILMHGIAHELPEEEAHPSAAIANWLTSHIAQIGISPERIVIDPG
                     IGFGTTRPQDAAILADLDPLLELGLPVLIGLSRKRVVRHLYPDADRDQASAVLAHAAW
                     RNGAAILRLHELQPLQQILAQKN"
     gene            7750..8166
                     /locus_tag="PKI64_08325"
     CDS             7750..8166
                     /locus_tag="PKI64_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOE01051.1"
                     /translation="MGQYPVSVEKEAALLRRMAELGIHEEEVEEWFIRGGGPGGQKTN
                     KTASSVCLRHKPSGCEVRCGRERSQALNRFLARRELCEKVAAKIHGEKNARQQAREKI
                     RRQKRRRSRRQTAIMVDNKKKHGAKKALRRRPEPDA"
     gene            complement(8183..9463)
                     /gene="murA"
                     /locus_tag="PKI64_08330"
     CDS             complement(8183..9463)
                     /gene="murA"
                     /locus_tag="PKI64_08330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOE01052.1"
                     /translation="MARLIIRGGKAITGTFRPLGNKNAVLPMLAASVLTDEPLTLDNV
                     PQILDVENMLKLLAGLGVVISRRGHSVTLCARGLHTTTLDPALCAKVRGSILLAGPLA
                     ARHGRAILSAPGGDVIGRRRLDTHFEGLAQLGIRMQWHNNTFHLTRRRLRGAELLLDE
                     ASVTATENILMAATLAPGRTTIFNAACEPHVQDLGNLLTKMGARITGLGTNRLEVEGV
                     KTLRGARHRVGPDYIEIGSVLAAAAATGGALTVTELPDATTLQVMQRAFGRLGVTWRV
                     ADSRLILPARQRLRVQQDLGGAIPKMEDGIWPAIPSDLLSVALVLATQTKGEILFFEK
                     LFESRMYFVDRLLEMGATLVQCDPHRVVVSGPAKLRAQHITSPDIRAGMALIIAALCA
                     KGTTVIEQAEMVDRGYERIEARLRALGADIRRAE"
     gene            complement(9586..10200)
                     /locus_tag="PKI64_08335"
     CDS             complement(9586..10200)
                     /locus_tag="PKI64_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE01053.1"
                     /translation="MKRKSVGFTLIELLVVIAIIAMLAAILVPAVNKALVSAAMVQTT
                     SNGANIYKSAFAGQMDDVVLGGGYSAWPSNAVTSTEYFISLVTSGVMTVSFDFFSARG
                     LPARKTSDPSQFDGAGNAWNLVNYLSDPKEGTTWLYTRNGPDAVPMNNSPIQLKADTE
                     PFRDAGMVVVLKGGSAFSIKTKQLTGEYMNPAQDSAATLQVVGP"
BASE COUNT         2126 a         3341 c         2906 g         1961 t
ORIGIN      
        1 tttattgcaa ttatccagaa aggcagtagg gtataaccag tttcttgttc ggaaggatac
       61 ccatgaaaat ctcgacaaaa ggccgttacg ggttgcgcac gctgatggat atcgccctgc
      121 atcagggcaa gggcccggtc aatctcaacg acatcgccca gcgccagggc atctcggcca
      181 agtatctttg gcaaatcgtc aacctgctca aaaccgccgg ttttgtgcgc ggcacgcgcg
      241 gccccaaggg aggctatgtt ttgttgcgcc ccaccgccga catcacgttg ctggatgttc
      301 tgcaaactct cgaaggcccc ttctcgctgg tggagtgtgt ggatgatccc gaggtgtgcc
      361 agcgttccgg cgtctgtgtg gcgcattcca tttgggagga agtcagccaa gccattcgcg
      421 ccgcgttgca aaaaatcacg ttggccgaaa tcctgcgtcg ccaccattcc gccgatagcg
      481 cgcaccattt tgtgatttga gacgccgcgc gcgtcacacc agctcggcaa aaacggtgtc
      541 gctgatgaat agcgcatcgt cggccaaacg aatacgttcc ggggtcacca ccagcaatcc
      601 ctcgcgcccc agccgcgcaa tctccgcgcc gcgtaaatcg tcatagtcga atccggtcgc
      661 cgcgcggaac tcttcgcgcc cccaccccgc cagccggcgc agtcccatca ccagcgtctc
      721 gcgcgccttg gccgctggtt ccagttgctc ggtgaattcc cgctgccaat tgggcagggt
      781 gtttgtgttg ccccagcgag ccccttgcca atgcgaatgg gccgccggcc cgaccccgat
      841 atattccccc ccgctccaat acaacagatt ctgccggcac tcatgccccg ggcgcgcgaa
      901 gttggagagt tcatagtgga ccaggcccgc ggcgggcaag cgctgccgca gccagtcaaa
      961 ttgcgcccgc tgatcatctt ccaccaccgc caaccgccct gccgccgctt cacgcgccca
     1021 cggtgtgcct tcttcgattt ccagcgcata gcccgaaata tgctccggcc ccagttccag
     1081 cgcggcttcc acatcggcca tgaagtcatc cgccgccacg ccggggatgc catacatcag
     1141 gtccagccca atgttgtcaa atcccgccgc gcgcgccatc gccacgcatt cgcgcgtctc
     1201 aacggattga tgaatccgcc ccaacttctg caaggtggga tccgtgaacg actgcgcgcc
     1261 aatggaaaga cgattcactc ctgcctgacg caagagctcg gccatcggcc tcgtcaacgt
     1321 gccgggattg acctcgcacg tccattcttc aatttgcgtc aaagggacat gcgcgcgcac
     1381 cagatccaga acactcgcca cgtcgtccgg cgaaagtgcg gtgggcgttc ccccgccaaa
     1441 aaatatgctc cgcggcacaa acccgcgcgg cagactttct agttcgcgaa ccaaaccctc
     1501 ccgccatgct gccacggtgg ccgaccgggg ggtttccgaa aaaaaagcgc aatagggaca
     1561 cttgcgcacg caaaacggaa aatggatgta gaggccgggg ctcatgcccg ccggatggtt
     1621 caaggatttt ccagctcgtg cagttcgcgc aagatgcgct cggcttccgc ggtgtccagc
     1681 cgcgtcatcg cgaagcccac gtgcaccagc acatagtcgc cgatttgcgc ttccggcaaa
     1741 taggccagcg ccacttcctg gcgcacgccg tcatagtcca ccgtgccgcg ccggtcgaaa
     1801 ccttcgccct cccgaatggc tacaattttt cccggaatag ccaggcacat ggcgtcagac
     1861 tcccccctct gccatgcccg gcggcggctg ggtcattttg gtcacccgca gcgggaaccg
     1921 cattttgatg tcgttgtcca gcagcacttc cacccaatga atgccggctt catggaactc
     1981 cgtgttgatg aacagttcca cgttggttgc cgtggcttcc gcggtgggca gtttgacggg
     2041 gatgtcccgg ccttcgatca tcacggtgct catgtccggc cgtaaaatcc gggtgtgttg
     2101 ccggaattcg ccctcgcccg agcaccagcg cgtcaccaaa aacatccgcg ggtgccggaa
     2161 gggaaacgac ggactgccca actggtcgaa caggccaatc aaaataaatt tgccgctccg
     2221 ctcctggcgg acgtcatcgc acaacaacgt gcattgcaaa tcaggggtca tcggcatttg
     2281 tgcctacctt tccacatcgg gagcgcacgc ccacgctcgc caccgcaatc caccatacct
     2341 gcgcgccacg cgcttggcaa tgctccgcct tgcgccgccg ccggagttca ccccgccgcc
     2401 tcacggcaca atttccacct tgattaaatt actggcgccc gtcttgccca gcggaaagcc
     2461 ggcggtgcaa accacggaat cgccgggacg taccaccccg gtggccttcg ccgcgcggtg
     2521 catctccgca aacaggtcaa tcagcaaatc cacccgttgg atcatcatcg ggcgcacccc
     2581 ccacaccaac gataagcagc ggaaagcgtc cacctccgtc gtgccggcca aaatcggttg
     2641 caccgggcgc gtcttggccg caagcttcgc cgtgccgccc gtttgcgtgg caatcaccaa
     2701 agctttcgct tcgatggttt cggccagcga gcacgccgcg cgggtcaatg cctcggactc
     2761 atccgccgtt tgcacctgct gcaagcgctg ccaccaggct gcgcgcaaaa atcctttttc
     2821 ggcttccacc acaatccgcg ccatggtcgc caccgcctcc accgggtaat tccccatggc
     2881 gctttcttct gacagcatca ccgcatcggt tccctccagc accgcgttgg tgacatccgt
     2941 cacctccgcc cgtgtggggc gtggcgcatc cgtcatgctc ttgagcatct gcgtggccgt
     3001 aatcaccggc ttgccggcaa agttggcttt ctcaatgatc atccgctgtg cggtgggcac
     3061 cttttccggc ggaatatcca cccccaaatc gccgcgcgcc accatcacgc catccaccgc
     3121 ggcaataatg gtatccaagt cctgcaacgc ctcgtgcttt tcaatcttgg caatgatcgg
     3181 ccggatgcgc ccttgtgcgc gcatgtaggc ccgcgccact tcaatgtccg ccgccgaacg
     3241 cacaaacgat agcgccacaa aatccacgcc ctgcgccaaa ccaaacgcca gatcgtcgta
     3301 atccttcttg gtcagaatcg gcgcgcggat gctccgcgaa ggcaaattga ttcccttgtg
     3361 gctgctcagc cgcccgccca aaatgacccg gcagtggatg tccggcccct ccactttttc
     3421 cactcgcagt tccagcgccc catccgccag cagcaggata tcgcccactt gcacatcctg
     3481 cggcaactcc ggataggtca gccccacttc gtgctcatcc cccggcacat cccgcgccgt
     3541 cagcacaaac tccgctccgg tctgcaactg gatgctgccg caagcgaacg cccccgtccg
     3601 gattttcggc ccggccaaat cttgcaagat ggcaatcggc agcccttttt catcggcgat
     3661 ctgccgcagc agcgcgatct tggccgcatg gtccgcgtgc gtcccgtgcg aaaaattgag
     3721 ccgcgccaca ttcatcccgg catccatcat ccggcccaga acttccggcg actccgacgc
     3781 cggaccaatc gtgcacacaa ttttggtttt tctcatacgc ccctcactct ccacgatttt
     3841 ttcggctagt caagcgattt acccgtggcc cagccaaccc agcaatcctg gcacatcgcc
     3901 ataatccgcc accgccagcg cggctcccgc ctcccgcaaa atccccacat catgccgtcc
     3961 cgtcgccacc cccaacacgg gaatccccaa agccactccc gcttgcacgt catagggcgt
     4021 gtctccaacc aagcagacct catccaattc cgccacaacc cccatctgct tcatttcgcc
     4081 cagcgctgcg cgggcaatct ccgctcgatc cgcatgatga tccccaaacc cgccgaacga
     4141 aaagaaatcc gccaacccca cgctccccag cttcagaaaa gcgcaatccc tgatgttccc
     4201 cgtcaccagc cccagttgtg ccccgctttc cgccaaggcc gagagtaacc ggcgcgcgcc
     4261 gttgatttcc cgcgctgggt gtttcgccag caacccccgc aattcttcgg ccacatggcg
     4321 aaatacctgt tcaatttcgg attcgtcgaa cttcaacccg cgccgagcca tcacctgatc
     4381 cagcacattg cgatccgtat tccccgcaaa cgcgatatac tccagctcgt cccgctcccc
     4441 cgtcagcttg gccaacccgc ggataaacgc ctcccgccca tggccatgcg tatccagcaa
     4501 cgtgccatca atatcaaaca aaaccacttt tttgcgccgc catgttttca tcggccccca
     4561 tcctactcga attaccacct cccttgccag ccccgcatcc ccatccgcag gaacgctccc
     4621 atcacgggta tacacaaggt atgcgccggg gttgctccat ccggcgcggc gtgatagggt
     4681 gccctctcaa atcgcaaaat catccactaa atgatcgcag ctcatggacc ccaaacaaaa
     4741 taatcccaaa gatccccaag gccagcggcg cccccccatg cccatgcggg cgttggtggc
     4801 ttggttcctc ctcattgcgc tggtggtcac ggtgttccaa ctggccactc aacacagcaa
     4861 caaagccacc agtctgccgt ataacccgga ctttctgacg ctcctcaagg aggggcggat
     4921 caacaagtgc gaagtggtga tggaagtatc cggccagagc ttcatccgcg gcgaactcaa
     4981 agaactggat gggaccggaa tcaaacagtt ccgggtggaa acgccggtga ctgaaaacct
     5041 ggtggaaaaa ctgacggcgg ccaaagtgcc gttcgagttc aagccgcaga atccgctggt
     5101 gtggcagatc gtctcgtcca cggttccctt cctgttggtg ttcgcgctga tctatttcct
     5161 tttctaccgc aacatgtccg gcagcgcgcg cggcgcattc aatttcggca aatcccgcgc
     5221 acgcctcatg acgcgcgaca ccaataaaat caccttcaag gatgtggcgg gcgcggacga
     5281 agcccgcgaa gaggtgcagg agatcatcga atttttgcgc gatcccaaga aattccagcg
     5341 gctgggcggg cgtatcccca agggtgtgct gctggtgggc ccgccaggca ccggcaagac
     5401 gctgattgcc aaggccatcg cgggcgaggc ccaagtgccg ttcttcagca tttccggatc
     5461 ggactttgtc gaaatgttcg ttggcgtggg cgcctcccgt gtgcgtgaca tgttcgaaca
     5521 aggcaaaaag aatgcgccgt gcatcatctt cattgacgaa attgatgcgg tgggccgctc
     5581 ccgcttttcg ggcatcggcg gcgggcacga cgagcgcgag caaacgctca acgccttgct
     5641 cgtggaaatg gacggcttcg acacgcaaga gggtgtcatc atcattgccg ctaccaaccg
     5701 acccgatgtc ctcgaccccg ccctgctgcg cccggggcgc ttcgaccgcc aaattgtcgt
     5761 ggacctgccc aacctgaccg gccgcgaggc catcctgaaa atccacgcgc gcaaggtcaa
     5821 actggatgcc accgtccaac tgcgcaacat cgcccgtggg acaccgggct tctccggtgc
     5881 ggacctcgcc aacctgatga acgaagccgc gctcctcgcc gcccgccgtg gcgcggaaac
     5941 catccacgaa aaagatttgg aagaggcccg cgacaaagtt cgttggggcc gcgagcgccg
     6001 ctcgcgcgta ttggacgaca aggaaaagcg tctgacggcc tgccacgaag cgggccacgc
     6061 cattgtgctg gcgctgacgc ccggcgcgga gccgttgcat aaagtcacca tcatcccgcg
     6121 tggccaggcg ctcggcgcga ccttccaact gccggaaaag gaccgctaca ccgaaggccg
     6181 tgaacggctg ctgggcatga ttaccggcac catgggcgga cgggtggccg aacaactgtg
     6241 cttcggcgaa atcaccaccg gcgcggcgtc cgacatcaag caggccacgc acatcgcgcg
     6301 catgatggtc accgaatggg gcatgagcga cctgctcggc ttccagcact tcggcacgcg
     6361 cgaggagctg ctcttcctcg gccgcgaagt atcgcgcacc aacgaaatgt ccgaagaaac
     6421 cgcgcgtaaa attgatgccg aggtcaagcg gatcattgac gagtgctacc aaaaggccgt
     6481 ggacattctt acccacaacc gtgccaagct cgatctgctg acgagcctgc tcattgagcg
     6541 cgagacgctc gatggccgcg acgtggaaga aatcctcaag catgaccgca ttcttacggc
     6601 ggcggaacgc aatgccgaag atgccgtcaa ggaagcggaa acgccggcgg cccctgctgc
     6661 ggagtaaccg cggggcgagg ttttccacgc catggaaaaa attgcccaag gttttccaca
     6721 ctgtggaaaa taaggcgtca agttttccac actgtggaaa aaagttttcc acgccgtgga
     6781 aaaatcggcc ggaattttcc acactatgga aaaaagtttt ccacactgtg gaaaaatccg
     6841 cggccccggc aatacccgcc ccccccgtcc ccgttttgat ggggattttg aacctcacgc
     6901 ctgattcgtt cacggatggc ggccaatttc tggatccggc cgcggccgaa gaacaagctt
     6961 acaaactggc ggcagatggt gcgcatattt tggacattgg cgcggagtcc acccgccccg
     7021 gccatgaatt gattccggca gcggaagagt tgcgccgcct gctgccggtg ctggatcgcc
     7081 tggccggaaa gttgcgcatt ccgatttcga ttgataccag caaaccggaa gtggcgcgcg
     7141 cggcgttcga ccgcggcgcc acgattctca atgacgtggc tgccctcacg cggcccggaa
     7201 tgttggattt tgcgcgggcg gggcacttcc ctgtaatttt gatgcacggc atcgcccacg
     7261 aattgccgga agaagaggcg catccatcgg cagccatcgc caactggctg acgtcccaca
     7321 ttgcgcaaat cgggatttcc cctgaacgca ttgtgattga cccgggaatc ggattcggaa
     7381 caacccgtcc acaggatgcc gccatcttgg cggatttgga cccgctacta gaattgggat
     7441 tgcccgtttt gatcgggctg tcgcgcaagc gggtagtgcg acacctgtat cccgacgctg
     7501 atcgtgacca ggccagtgca gtcttggcac acgcagcatg gcgcaacgga gccgccatcc
     7561 tgcgcctgca cgaattgcaa cctttgcagc agattttggc gcagaaaaac tgaaatgcct
     7621 ccattttatt agcacctata acgcaaagcg aatgtttcac ttattgcaat aagtgaattc
     7681 actttttgca atatgtgaag cggcttttcc gttttcattt gggagaaact gaactaggct
     7741 tttcctgcca tgggtcaata tccggtcagc gtcgaaaaag aagccgcgtt gctgcggcgg
     7801 atggcggagt tgggcattca cgaggaagag gtggaggagt ggttcattcg cggcgggggg
     7861 ccgggcgggc agaaaacaaa caagaccgct tccagcgttt gcttacggca caagcccagc
     7921 ggctgtgaag tgcgttgcgg gcgagagcgc tcgcaggcgc tgaaccggtt tttggcacgg
     7981 cgggagcttt gtgaaaaagt tgccgcgaaa attcacgggg aaaaaaacgc ccggcaacag
     8041 gcacgcgaaa agattcgccg ccagaagcgg cggcgttcgc gccggcaaac ggccatcatg
     8101 gtggacaaca agaaaaagca tggggccaag aaggcgctgc gacggcggcc ggagccggac
     8161 gcgtaaatca accggggagt atctactcgg cgcggcggat atccgcgccc agcgcgcgca
     8221 accgcgcttc gatgcgttca tagccgcggt ccaccatctc ggcttgctcg atgacagtgg
     8281 tgcctttggc gcacaaggca gcgatgatca gcgccatgcc ggcgcggatg tccgggctgg
     8341 taatgtgctg ggcgcggagt ttggcagggc cgctgaccac cacgcggtgc ggatcgcatt
     8401 gcacaagcgt ggcgcccatt tccagcagtc ggtccacaaa atacatccgg ctttcgaaca
     8461 gtttttcaaa aaatagaatc tcgcccttgg tctgggtggc gagaacgagc gccacgctaa
     8521 gcagatcgga cgggatggcc ggccagatgc catcttccat cttgggaatg gcgccgccca
     8581 aatcctgttg gacgcgcagg cgttggcggg caggaagaat cagccggctg tcggccacgc
     8641 gccaagtgac acccagccgg ccaaacgcgc gctgcatcac ttgcaaggtg gtggcatcgg
     8701 gcaattcggt gacggtgaga gcgccgccgg tggcggcggc ggccgccagc acactgccga
     8761 tctcgatata atccggtccg acacgatggc gcgcaccgcg gagagttttg accccttcga
     8821 cttcgaggcg gttggtgccc agtccggtaa ttcgtgcgcc cattttggtg agcagattgc
     8881 cgaggtcctg cacgtgcggc tcacaggcgg cattgaaaat cgtggttcgg ccgggggcaa
     8941 gcgtggcggc catcaggata ttttcggtgg cggtgacgct ggcttcgtcg agcagcaatt
     9001 ccgcgccgcg caggcggcgg cgggtgagat ggaaggtgtt gttgtgccac tgcatgcgga
     9061 tgccgagttg ggcgagaccc tcgaagtggg tatcgagccg gcggcggccg atgacatcgc
     9121 cgcccggtgc ggacaggatg gcacggccat ggcgcgcggc caagggaccg gccagcaaaa
     9181 tggagccgcg gactttggcg caaagggcgg ggtccagcgt ggtggtgtgc agcccgcgcg
     9241 cgcacaatgt gacggaatgc ccgcggcggg aaatgaccac gccgaggccg gccagcagct
     9301 tgagcatgtt ctcgacatcg agaatctgcg gcacgttgtc gagtgtcagg ggttcatccg
     9361 tgaggacgct ggcggccaac atgggcagca cggcattttt gttgcccaac gggcggaagg
     9421 ttcccgtgat ggcctttcct ccgcgaatga tcaaccgcgc catgcgtact tctctccctt
     9481 ggcgggcgtt tcagtcaatg gccacgccac gccttgccac cctattcaac agaaaaggcc
     9541 gggaccgcgc aaggtgtccc ggcctcatcc agaccaaatc cgaccttatg gaccgacgac
     9601 ttgcagggtc gccgcactat cctgagcggg gttcatgtat tcgcccgtca attgcttggt
     9661 cttgatggaa aatgccgaac cgcccttgag tacgaccacc atgccggcat cccggaaagg
     9721 ttcggtatcg gccttgagct ggatcgggct gttgttcatg ggcacggcat cagggccatt
     9781 gcgagtgtag agccaagttg tgccttcctt ggggtcggaa aggtaattga ccaaattcca
     9841 cgcattgcct gccccgtcaa actggctggg atcggatgtt ttgcgcgcgg gaaggccgcg
     9901 ggcggagaag aagtcgaagg acaccgtcat gacgccggag gtgaccaagc tgatgaaata
     9961 ttcggtggaa gtgacggcat tggaaggcca agcggaataa ccaccgccga gcaccacgtc
    10021 atccatttgc cccgcaaagg ccgacttata gatattcgcg ccattggagg tggtctgcac
    10081 catggccgca ctgaccagcg ccttgttgac ggcagggacg agaatggcgg cgagcatggc
    10141 gatgatggcg attaccacca gcagttcgat cagggtaaaa ccaacggact tgcgcttcat
    10201 aatgacgggt ctcctttatc ggtagtcttg aagggtatct tactttatct gcacgacccg
    10261 tcaaccatga agatttacgc tgccgtgtag ttttcaaacc cgcacttttt tgtagttagc
    10321 aaagtcgcac tttt
//
LOCUS       DAOPNP010000091         9858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_7812_length_9858_cov_9.047740, whole genome shotgun sequence.
ACCESSION   DAOPNP010000091 DAOPNP010000000
VERSION     DAOPNP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7812_length_9858_cov_9.047740"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..578)
                     /locus_tag="PKI64_08340"
     CDS             complement(165..578)
                     /locus_tag="PKI64_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOE01054.1"
                     /translation="MNKTIKMLFHADADRNLGLLILRVFLGASLLTHGWAKMFGGLAK
                     HAQFVSSLGMPAPHVMAFLSAFAESFAALFLIVGFLTRPAALMVVVNMLVAICVVHLH
                     DGFSKQEAAWLYLVPALFFVLKGAGYWSVDRTLSR"
     gene            complement(571..1722)
                     /locus_tag="PKI64_08345"
     CDS             complement(571..1722)
                     /locus_tag="PKI64_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE01055.1"
                     /translation="MKKRVIILGGGFAGQQAARALAATDADITLLDPSPVATMLPLLP
                     DLAGGWVRETIAYRPLAQRLPRRVHLIPEAATEIHLAAKTVTLRDQTLPFDHLLIATG
                     STPNLRRMIFPREKIHVLGNLADARRIREEFTAYLQEADAPHILVSGTGYTGLELAVS
                     LLFRAKAAGKKCGLTIVDPNPILLPSLPENHRNYVLRFLQSHGAEVRLGVAVETYDGH
                     RATAGGQTYENPFFCWTVGSAFPEVTIHGAGERARDGRLHVQPDLSLPGHPNIFVAGD
                     AAAITQNDRVLRKAVNFAYYGGRHAGRNIARQLRGRPTRPFRPVDLGWIVPFHATSIG
                     RLPGGLWVRGPLGVRLHHLMCGVRNYSFRNFAGCARVALTLYKGETQHE"
     gene            1910..3256
                     /gene="trkA"
                     /locus_tag="PKI64_08350"
     CDS             1910..3256
                     /gene="trkA"
                     /locus_tag="PKI64_08350"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOE01056.1"
                     /translation="MRIVIIGGGQTGAHLAETLSPDGHDVVLIDSSAEVLAELDAHLD
                     LMTIQGDGANPETLEKADLERADLVVAVTGRDDTNILACIFARQRGGARTVARVSSSA
                     YTTSRHLDFRALGVDLLVNQYDEYARDLYNVLRLPGSIEVADLLGERVMVVGMPVHMD
                     SPLLGQALKNLAGKEWLKRIRFMALVRADQVMTPHGETTFLVGDDVYFSGAPDAVADF
                     LQKAWPEYRPFQRVVIAGGGDLGLELARMLEHTRMQIVLVEQDEERADYCSAELNRTL
                     VIKGDALDQETLAGIGDATDMAYVAATGSDEHNIIGCLVAEKYGARFTAAQVGKKEYI
                     GIVNQLRLLDRIVDPRLAMINSILHFVRGKNVQAAAVLSRLPGELIELRLTAQHEWSG
                     KAIKDLKLPAGVIVVTVLRQDQVKTAIGDLVLAVGDRVVVYCLPAVRPKMESLFTS"
     gene            3267..4718
                     /locus_tag="PKI64_08355"
     CDS             3267..4718
                     /locus_tag="PKI64_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOE01057.1"
                     /translation="MNFKPVFRVIAALLGVMALLMGLCGGVSYWQGEAAVTWRALLHS
                     AGGTLVVAGILWALTRNRQELSRRDGLGVVTFGWLLAGLAGAAPYWLSGVIASPMAAI
                     FESVSGLTTTGATVIPVLEGVPRGILLWRALTQFIGGMGVLVLVMAVLPFSGAGGMQL
                     FRAEMPGPTKDRIEPRMASTAKMLWGVYLILAAVLLVLLRAGGMTWCDAICHSLTTVA
                     TGGFSTRTASIAAFDSVYIETVLTFFMLLCGVNFAVHYRALRGDVAAWWRDAEARFFL
                     GIFFVAAVAATFGLHHARAGLAWSSAAREAAFTCASLITSTGFSTGNFDLWPALLKWM
                     LALLMLMGGCAESTAGGIKAGRIQVLLKTVIREVRLFMQPQAVIPVKIGRKVLNDELL
                     RSILAFVLLFFLILVGGIAALLPFMPDIQTAASSAIASLSNVGPGFAGVGPASSYAAI
                     PAGGQGILAVLMLVGRLELLTVLAILMPSFWRK"
     gene            4718..5440
                     /locus_tag="PKI64_08360"
     CDS             4718..5440
                     /locus_tag="PKI64_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01058.1"
                     /translation="MARPPLFPDLAASAHLADVQVRQTLALGLLPLGVAVLLMAAGFP
                     PTGLAVTAGLVMALMALLWRQWWRAQRGRTRGFSTLHFLARYFFVAACPVLLWLVFGG
                     TILELTGAFPPLLLALLLAIHPVGLILRARSGPDPSLSPRNELARIICQQTEMVLLVL
                     ALAGLLSGAVLDANRDYPTEPLPLLIAIWLLAVVVLVAGVVTSYAHASQLRGHPGPPQ
                     PLDDPPPAPPADQPARYGSDRF"
     gene            5520..7307
                     /gene="aspS"
                     /locus_tag="PKI64_08365"
     CDS             5520..7307
                     /gene="aspS"
                     /locus_tag="PKI64_08365"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE01059.1"
                     /translation="MKMRTHTCGQLRATDDGAEVRLCGWVDSVRDHGGLIFLDLRDRY
                     GVTQCVFDPQDSQAAWDAAQACRGEYVVQITGQVRQRPADMVNPNILTGGMEVRATGI
                     SVLNKAHTPPFPLEDRQAELVSEDLRLEYRYLDLRRPAMQQALALRHRLIQAARNYFD
                     EQQFVEIETPILTKSTPEGARDYLVPSRVCPGSFFALPQAPQQYKQLLMMAGMDRYVQ
                     IARCFRDEDLRADRQPEFTQIDLEMSFVTADDIIATVDGLLARLMAAAELPVPELPLP
                     RMTYREAMDRFGSDKPDLRFGMEIVDLANVFKGTQFKVFGRALESGGVVKAINAKGLA
                     HAPIKLVEEDWTGLAKEGGLGGLAYIRVQEDGTWKSPIVKFFTEAEKTSLQLLLEIQP
                     GDLILFGADTASKVLPVLGRIRLLAAAWAKIIPDNVFKFTWVTDFPLFERNEEGRLVS
                     VHHPFTSPMPEDIPLLDTDPEKVRAQAYDIVLNGTELGGGSIRIHDPQFQARMFQLLG
                     LPEAEIQSRFSHLIRALEYGAPPHGGLAIGADRLVMLLAGRHSIRDVIAFPKTQKAMD
                     LMMNAPAPVDDKQLRDIHIKLAVAPKVDA"
     gene            7304..8092
                     /locus_tag="PKI64_08370"
     CDS             7304..8092
                     /locus_tag="PKI64_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE01060.1"
                     /translation="MNPAGQLVLNAIRFEYESAAWTLAVPTLTLGSEPLCAIIGPNGS
                     GKSTLLKVAAGLLRPAAGEARWQGRRVADYSRRTLAQQVGYLPQECPVLFDYTVEQVV
                     SMGRHAHGGVLELTTPGDRAAVDRALADVRLMDYRQRRLSQLSGGERRRAWLAAALAQ
                     EPAILLLDEPTQALDVHQAAAMMEILTARAAEGLRVVAVLHDLNLAALFCPRLVLLAG
                     GAVVADGPPAELLTPDRLRAVYGDKLDVVPHPRSGQPLVLAATS"
     gene            8089..9066
                     /locus_tag="PKI64_08375"
     CDS             8089..9066
                     /locus_tag="PKI64_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE01061.1"
                     /translation="MKRNQIILLGGLSLLCLLLLPWVGPERLSLRGLLAWGGDWTADQ
                     IIFVQLRLTRVVLGLLAGGALAMSGATLQVLFRNPLAEPWTLGVAGGASLGAFIARLC
                     PICWWAWGPFHSGQLLALLGAAGALLLVGAVARRAGGAGTPALLLAGITLSVVCGGAI
                     MMISGFVSPWKLAEFQRWLLGGLDAASWGDVAALLLLVVPGGAILLALARHYNPLALG
                     EDMAAGQGVDVAAVRCWTAIGAGVAAAGVAAVAGPIGFIGLLAPHAVRRLVGPDMRRV
                     VPASFLLGGSLLAACDALGRWAAAPLEMPVGALTAVLGGPVFLWLLARR"
     gene            9116..>9858
                     /locus_tag="PKI64_08380"
     CDS             9116..>9858
                     /locus_tag="PKI64_08380"
                     /inference="COORDINATES: protein motif:HMM:NF022760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE01062.1"
                     /translation="MNPFEHFVLTRCNAPLKAADTAAEHNNDWLTRRFDLFERVCLPS
                     MQRQLEGAYQWLVFMDWATPVHFKERMAALSVRHEFLRPVYCSHFDEATALAEIRRRE
                     TAGRARVTTQLPCAAALHPRMVEQVHEAVRQHIASHDLQRGFAISFPLGCCERKGDFY
                     LRRERANPFISFVSAPETERTALAGLADVPVVTRTRRPLWCEVLQDDTDATKPCGIYW
                     PMGGSSEFAPGITNGFRRGALWQGAEMVR"
BASE COUNT         1626 a         2900 c         3392 g         1940 t
ORIGIN      
        1 ctcgccaacg cggtgtccgt caaaacggac ctcttgacat gccttctcat aagagcgtgg
       61 aaaaatatcg tcctcgcgct cctgattttt ccacagtgtg gaaaaaagtt tatcccgcgg
      121 aggcaggatt ttccacacta tggaaaagta aattattcgg gggattagcg ggagagggtg
      181 cggtccacgg accagtagcc ggcgcctttg agtacgaaaa acaacgcggg caccagatag
      241 agccaggcgg cttcctgttt gctaaagcca tcgtgcaggt gcaccacgca aatggcgacg
      301 agcatattga cgaccaccat gagcgccgcg ggtcgggtca aaaaaccgac gatcaaaaag
      361 agcgcggcaa atgactcggc gaacgcggac aggaaggcca tcacgtgcgg cgccggcatg
      421 ccgaggctgc tgacaaactg cgcgtgcttg gccaagccgc caaacatctt ggcccagccg
      481 tgggtcaaga gcgacgcgcc tagaaagacg cgcaggatca acaggccgag attgcggtcg
      541 gcatccgcgt ggaagagcat ttttattgtt ttattcatgt tgggtttctc ctttgtaaag
      601 ggttaaggcc acgcgcgcgc agccggcgaa gttgcggaaa gaatagttcc gcacgccgca
      661 catcaggtgg tgcaggcgca cgcccagcgg gccgcgcacc cacagtccgc cgggaaggcg
      721 cccgatgctg gtggcgtgga aaggaacaat ccagccaagg tccaccgggc ggaacggacg
      781 ggtgggccgg ccgcggagtt ggcgggcaat gttgcgcccg gcgtggcgcc cgccgtagta
      841 ggcaaagttc acggccttgc gcaaaacgcg atcgttttgc gtgatggccg cggcatcgcc
      901 cgcaacaaag atgttgggat ggccggggag ggaaagatcg ggttgcacgt gcaagcggcc
      961 gtcgcgcgcc cgctcgccag cgccatggat ggtcacctcc gggaacgcgg aaccgaccgt
     1021 ccagcagaag aacggatttt cgtaggtttg gccgccggcc gtggcgcggt ggccgtcgta
     1081 ggtttccacg gccacgccga ggcggacctc cgcgccgtga gattgcagga aacgcagcac
     1141 atagttgcgg tgattctcag gcagggaggg gaggagaatc gggttggggt ccacgatggt
     1201 caggccgcat tttttgcccg cggccttggc acggaagagc aggctcacgg cgagttccaa
     1261 accggtatag cccgtaccgg aaacgagaat atgcggggca tctgcctctt gcagataggc
     1321 ggtgaactct tcacgaatgc gtcgtgcatc ggcgaggttg ccgaggacgt ggattttttc
     1381 gcgcgggaaa atcatgcggc gcaggttggg ggtcgagccg gtggcaatga gtagatggtc
     1441 gaaggggagg gtctgatccc ggagtgtcac ggttttcgca gccaaatgga tttcggtggc
     1501 ggcctcggga ataagatgca cacggcgcgg gagccgctgg gccagcggac ggtaggcgat
     1561 ggtctcccgt acccagccgc cggccaaatc aggcagcagc ggcaacatgg tggcgaccgg
     1621 actggggtca agcagcgtaa tgtcggcatc cgttgccgcc aacgcgcggg cggcttgctg
     1681 gccggcaaaa ccaccaccta aaataatgac ccgttttttc atagtccagt aaactacttc
     1741 acaatgctga aaatgcaaaa aacggttttg agctaatgaa tttgatgggc aacccattgc
     1801 tgttgcgcga gttggactcg cggcggaaga gagttgaccc ttcccccttg ccagcgctgt
     1861 gctgatggag gatactgccc catgttcttc aaaacagaac ggacgagcta tgcgcattgt
     1921 gattataggc ggcggccaaa cgggcgcgca cttggcggag accctttccc ccgacgggca
     1981 cgacgtggtg ttgattgact catcggccga ggtgctggcg gaactggacg cgcacctcga
     2041 tttgatgacg atccaaggcg acggcgccaa ccccgaaacc cttgaaaagg ccgatttgga
     2101 acgggccgac ttggtggtgg cggtcaccgg ccgcgacgac accaacattc tcgcctgcat
     2161 ttttgcccgg caacgggggg gggcgcggac ggtggcgcgg gtgtcgtcct cggcctacac
     2221 caccagccgg cacctcgatt ttcgggcgct gggggtggat ttgctggtga accagtacga
     2281 tgaatacgcg cgcgatttgt ataacgtcct gcgcctgccg gggtccatcg aggtggccga
     2341 tctgctgggc gagcgggtga tggtggtggg gatgcccgtc catatggata gcccgttgct
     2401 ggggcaggcg ctcaaaaatc tggcgggcaa ggagtggctc aagagaatcc gcttcatggc
     2461 gctggtccgg gcggatcagg tgatgacccc gcacggcgaa accacgtttt tggttggcga
     2521 cgatgtctat ttttccggcg cgccggatgc tgtggccgat ttcctgcaaa aagcatggcc
     2581 ggaatatcgc ccgttccagc gggtggtgat tgccggcggc ggcgatctgg gcctggaact
     2641 ggcccggatg ctggagcata cccggatgca aattgtgctg gtggagcagg acgaagagcg
     2701 cgcggattat tgctcggcgg aattgaaccg cacgttggtc atcaagggcg acgcgttgga
     2761 tcaagaaacc cttgccggca tcggcgacgc caccgacatg gcctatgtgg ccgctaccgg
     2821 cagcgacgaa cacaacatca tcggctgcct cgtggcggaa aaatatggcg cgcgcttcac
     2881 cgcggcccag gtgggcaaaa aggaatatat cggcatcgtc aaccaactgc ggctgctgga
     2941 ccgtatcgtg gatccgcggc tggcgatgat taattccatc ctgcattttg tgcgcggcaa
     3001 gaatgtgcag gcggcggcgg ttttgtcccg cttgccgggc gagttgattg agcttcgcct
     3061 gaccgcgcag cacgagtggt cgggcaaggc catcaaggat ctcaaactgc cggccggggt
     3121 gattgtggtg acggtgttgc ggcaggatca ggtcaagacc gccatcggtg atttggtact
     3181 ggcggtgggc gaccgcgtgg tggtgtattg cctgccggct gtgcggccca agatggaatc
     3241 gctctttacc tcgtgagggg aacgtcgtga atttcaaacc ggtattccgg gtcattgccg
     3301 cgctgctcgg cgtgatggcg ctgctgatgg ggctttgcgg cggcgtttcg tactggcagg
     3361 gggaggccgc ggtgacatgg cgcgccctgc tgcattcggc aggcggcacg ctggtggtgg
     3421 cggggattct ctgggcattg acgcgcaacc ggcaggagct gtcgcggcgc gatggcctcg
     3481 gcgtggtgac gttcggctgg ctgctggccg gactggccgg cgccgcgccc tactggcttt
     3541 ccggcgtgat tgcctcgccc atggcggcga tttttgaatc ggtttcgggc ctgaccacga
     3601 cgggcgccac ggtgattcct gtgctggagg gcgtgccacg gggcatcctg ctctggcgcg
     3661 cgctgaccca gtttattggc ggcatgggcg tgctggtgtt ggtgatggcg gtactgccgt
     3721 tttccggggc gggggggatg cagttgttcc gcgcggagat gccggggccc accaaggacc
     3781 gcatcgagcc gcggatggcc tccaccgcca aaatgctgtg gggggtgtac ctcattctgg
     3841 cggccgtgct gttggtgctg ttgcgcgccg gcggcatgac gtggtgcgac gccatttgcc
     3901 attcgctaac cacggtggcc accggtggat tctccacgcg caccgccagc attgcggcct
     3961 ttgacagcgt atatatcgag accgtgctga cgttcttcat gctgttgtgc ggcgtcaact
     4021 ttgccgtgca ctaccgtgcg ctgcgcggcg atgtcgcggc gtggtggcgc gacgcggaag
     4081 cgcggttctt cctcgggatt ttttttgtgg cggcggtggc cgcaacattc gggctgcatc
     4141 acgcgcgggc ggggttggcg tggtcgagcg ccgcgcggga ggccgcgttc acttgcgcca
     4201 gcttgattac ctccaccggg ttcagcaccg gcaactttga tctctggcct gcgctgctga
     4261 aatggatgct ggcgctcctg atgctgatgg gcgggtgcgc cgagtccacc gcgggcggca
     4321 tcaaggccgg gcgcattcaa gtgctgttga agaccgtcat tcgtgaagtg cggctgttca
     4381 tgcagccgca ggcggtcatt ccggtcaaaa tcgggcgcaa agtattgaac gatgaactgc
     4441 tgcgctccat cttggctttt gtgctgctgt tcttcctgat tctggtgggc ggcatcgcgg
     4501 cgttgctgcc gtttatgccg gacatccaga cagcggcctc gtcggctatc gccagcttga
     4561 gcaacgtggg gcccggattt gccggcgtgg ggccggccag ctcttatgcg gccatccccg
     4621 cgggggggca ggggattctg gcggtattga tgctggtggg ccgcttggaa ttgctcacgg
     4681 tgttggccat tttgatgccg tcgttctgga gaaagtgatg gcgcgtccgc cgttgtttcc
     4741 ggatttggcg gccagcgcac acttggccga cgttcaagtc cggcaaacat tggcgctggg
     4801 gctgttgccg ctgggtgtgg ccgtgctgct gatggccgcg ggcttcccgc ccacgggctt
     4861 ggccgtaacg gcgggactgg tcatggcact gatggcgctg ctctggcggc aatggtggcg
     4921 cgcccagcgc ggccgcacgc gcggtttctc cacactgcat tttctggcgc ggtatttttt
     4981 cgtggcagcg tgtcccgtgc tgctgtggct ggtgttcggc ggcaccattc tcgaactgac
     5041 gggagccttc ccgccgctgt tgctggcgct gttgctggcc atccatccgg tgggcttgat
     5101 cctgcgcgcg cggagcgggc cggacccgag cctttcgcca cggaatgaat tggcgcggat
     5161 tatctgccag caaacggaaa tggtgctgct ggtgctcgcg ctggcggggc tgttaagcgg
     5221 cgcagtactg gatgccaacc gcgattaccc cactgagccg ctgccgctgc tgatcgccat
     5281 ttggctgttg gcggtggtgg tattggtggc cggcgtggtc accagctacg cgcatgccag
     5341 ccagctccgc ggtcaccccg gcccgcccca gccgctcgat gatccgccac ccgcgccgcc
     5401 tgccgatcag ccggcgcgct acggctccga ccggttttga accgctggat ttgtttgcgc
     5461 gtggcgcgcg ggaaaggtac agtcggcact cattattttt tcagccaaag gatgcttcaa
     5521 tgaaaatgcg gacacatacc tgtggtcagt tgcgagccac cgatgacggc gccgaggtgc
     5581 gcctgtgcgg atgggtggat agcgtgcgcg atcatggcgg actcatcttt ttggatttgc
     5641 gcgaccgtta cggtgtaacg cagtgcgtgt tcgatccgca ggactcgcag gcggcgtggg
     5701 atgccgcgca ggcatgccgc ggggaatacg tggtccaaat caccggccag gttcgccagc
     5761 gcccggcgga catggtcaac ccgaacatcc tcactggcgg catggaagtg cgcgccaccg
     5821 ggatcagcgt cctgaacaag gcgcacaccc cgccgttccc gctggaggac cgccaggcgg
     5881 agttggtcag cgaagacctg cggttggaat accgctattt ggatttgcgc cgcccggcca
     5941 tgcagcaggc gctggccctg cgccaccgcc tgattcaggc cgcgcgcaac tatttcgatg
     6001 agcagcagtt tgtcgaaatc gaaacaccca tcctgaccaa gagcacgccg gaaggcgcgc
     6061 gcgattacct cgtgcccagc cgcgtgtgcc ccggctcatt cttcgcgctg ccgcaggccc
     6121 cacagcaata caagcaattg ctgatgatgg ccggcatgga tcgctatgtg cagattgcgc
     6181 ggtgtttccg cgacgaagat ctgcgcgccg accgccagcc ggaattcacg cagattgacc
     6241 tcgaaatgtc gttcgtcacc gcggatgaca tcatcgccac tgtggatggc ctgctggcgc
     6301 gactgatggc cgccgccgaa ctgcccgtgc ccgaactgcc cctgccgcgc atgacctacc
     6361 gcgaggccat ggaccgcttc ggcagtgaca agcccgactt gcgcttcggc atggaaattg
     6421 tggatttggc caacgtcttc aagggcaccc agttcaaggt gtttggccgc gcgctggaaa
     6481 gcggcggcgt ggtcaaggcc atcaacgcca aggggctggc ccacgcgccg atcaagctcg
     6541 tggaggaaga ctggacgggc ttggccaagg agggcggcct tggcggcttg gcctatattc
     6601 gcgtgcagga agacggcact tggaaatcgc ccatcgtcaa gttcttcact gaagccgaga
     6661 aaaccagctt gcagttgctg ctggagatcc aacccggcga cctgattttg ttcggtgcgg
     6721 ataccgcgtc caaagtgctg ccggtgctgg gccggattcg cctgctggcg gcggcgtggg
     6781 ccaagatcat tcccgacaac gtcttcaagt tcacgtgggt gacggatttc ccgctgttcg
     6841 agcgcaatga ggaaggccgg ctggtcagcg tccaccatcc gttcacttcg cccatgcccg
     6901 aagatattcc gctgctcgac acagaccctg aaaaggtccg cgcgcaagcc tacgacatcg
     6961 tgctcaacgg cacggagctg ggcggaggca gcatccggat tcacgatccg caattccaag
     7021 cgcgtatgtt ccagcttctg ggcttgcccg aggcggaaat tcagagccgc tttagccacc
     7081 tgattcgcgc gctggaatac ggcgcgccgc cgcacggcgg cttggccatc ggcgcggacc
     7141 gcctggtcat gctgctggcc ggacggcaca gcatccgcga tgtcattgcg ttccccaaaa
     7201 cgcaaaaagc catggacctg atgatgaacg cccccgcccc ggtggatgac aagcaactgc
     7261 gcgacatcca tatcaagttg gcggtggcgc ccaaagtgga cgcttgaatc ccgccggcca
     7321 gcttgtcctg aacgccattc gttttgaata cgaatcggcc gcatggacgc tggcagtacc
     7381 gaccctcacg ctgggctccg agccgttgtg cgccatcatt ggccccaatg gttcaggcaa
     7441 aagcaccttg ctcaaagtcg ctgcgggcct gttgcggccc gccgcgggcg aggcgcgctg
     7501 gcaaggccgg cgcgtagcgg attattcgcg ccggactctc gcccaacagg tgggctattt
     7561 gccgcaagag tgccccgtac tgttcgatta caccgtggag caagtcgtgt ccatgggccg
     7621 ccacgcccac ggcggcgtgc tggaactgac cacccccggc gaccgcgccg ccgtggaccg
     7681 cgcactggcg gatgtgcgcc taatggatta ccgccagcgg cggctttcgc agctgtctgg
     7741 cggtgagcga cggcgcgcgt ggctggccgc ggcgttggcg caggagcctg ccatcctgct
     7801 gctggacgag cccacccagg cgctggatgt gcatcaggcc gcggccatga tggagattct
     7861 gaccgcgcgc gcggccgaag gcttgcgcgt ggtggcggta ttgcatgacc tcaatctggc
     7921 ggcgttgttt tgtccgcggc tggtgctgct ggcgggcggt gcagttgtgg ccgatggccc
     7981 gcccgcggaa ttgctgacgc cggaccgcct ccgtgcggtg tatggcgaca agctggacgt
     8041 tgtgccgcat ccgcgctccg gacaacctct cgtcttggcg gcgacctcat gaagcgcaac
     8101 caaatcatcc tgttgggcgg gttgagcctc ttatgcttgt tgctgttgcc gtgggtgggg
     8161 cccgaacgct tgagcctgcg cggtctgttg gcgtggggcg gggactggac ggcggaccaa
     8221 attatttttg tccaattgcg cctgacgcgc gtggtgctgg ggttgctggc aggcggcgca
     8281 ctggccatga gtggcgcaac gctgcaagtg ttgttccgta atccgctggc ggagccttgg
     8341 acgctgggcg tggccggtgg tgcgagcttg ggcgcattca tcgcgcgtct gtgcccgatc
     8401 tgctggtggg cgtgggggcc gttccactcc ggacagttgc tggccttgct gggcgcggct
     8461 ggcgcgctcc tgctggtggg ggccgtggcg cggcgcgcgg gtggcgcggg cacaccggcg
     8521 ctgctgctgg ccgggatcac gctcagcgtg gtttgcggcg gagccatcat gatgattagt
     8581 ggatttgtgt cgccatggaa gttggcggaa tttcagcgct ggctgctggg cggattggat
     8641 gccgccagtt ggggcgacgt agccgcgctg ttgctcttgg tggtgcccgg cggcgcaatc
     8701 ctgctggcgt tggcccggca ctataatccg ctggcgctgg gcgaagacat ggctgcgggg
     8761 cagggcgtgg atgtggccgc cgtgcgttgc tggacggcca tcggtgcggg cgtggccgcc
     8821 gctggcgtag ccgccgtggc cggaccgatt ggcttcatcg ggctgctggc gccgcacgcc
     8881 gtgcgccggt tggttggtcc cgatatgcgc cgcgtggttc ccgcttcctt tctgctgggc
     8941 gggagcctgc tggccgcctg cgatgccctc gggcgttggg ccgccgcgcc gctggaaatg
     9001 cccgtgggcg cgctgaccgc ggtgctgggc ggccccgtat ttctctggct gctcgcccgc
     9061 cggtgattgc caaaaacgct tgtttgcgcg gcggggcgct ggcatccttc ggggcatgaa
     9121 cccttttgag catttcgtgc tgacccggtg caacgcgccg ctcaaagccg cggacaccgc
     9181 cgccgaacac aacaacgact ggctgacccg ccgcttcgac ttgtttgagc gggtatgcct
     9241 gccctccatg cagcggcaat tggaaggcgc gtatcaatgg ctggtcttca tggattgggc
     9301 cacgccggtt catttcaagg aacgcatggc ggcgttgtcg gtgcgtcacg aatttttgcg
     9361 tccggtctat tgctcgcact tcgatgaagc cacggcgctg gcggaaattc gccgtcgcga
     9421 aaccgctggg cgtgctcgcg tgaccacgca gttgccctgc gcggctgcgc tgcatccgcg
     9481 catggtggag caagtgcacg aggccgtgcg ccagcatatc gccagccacg atttgcaacg
     9541 cgggttcgcc atcagctttc cgctgggctg ctgcgagcgc aagggcgact tttatctccg
     9601 ccgcgagcgg gccaatccgt tcatcagctt tgtctcagcg ccggagacag agcgcacggc
     9661 gctggccggt ttggcggatg tgccggtggt cacgcgcacc cgccgcccgc tctggtgcga
     9721 ggtccttcag gatgacaccg acgcaacgaa accctgtggc atctattggc ccatgggggg
     9781 cagcagcgaa ttcgcgcccg gcatcaccaa cggcttccgt cgtggcgcgc tgtggcaggg
     9841 tgcggaaatg gtgcgcag
//
LOCUS       DAOPNP010000092         9772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_7885_length_9772_cov_9.535968, whole genome shotgun sequence.
ACCESSION   DAOPNP010000092 DAOPNP010000000
VERSION     DAOPNP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7885_length_9772_cov_9.535968"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..764)
                     /locus_tag="PKI64_08385"
     CDS             complement(27..764)
                     /locus_tag="PKI64_08385"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOE01063.1"
                     /translation="MFRCLSSLLAVGVIGARLAAAQTVVPPPAGTADTNATQPIVIYP
                     AGQEPQPEPSPVLWQGHVATLGAVTLDASTKTVTATGWVNQTAGLIEVLACGIQGKVH
                     ESVFVIPLNPLDLQAALLLTGLKSGPPMPAVGEGPPHGDPVNIFVDWQEGDETRTARA
                     EAFIWNNETDSVLPDTPWTFTGSMIKDGHFKAFAEESYVVSYWDPYAIINLPLPCGAD
                     DEILAVNTNIVPAYRTPITMRFVPAAP"
     gene            complement(725..1315)
                     /locus_tag="PKI64_08390"
     CDS             complement(725..1315)
                     /locus_tag="PKI64_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01064.1"
                     /translation="MRWGLIAILLAVCGGSVDAQTSEWPQLSPEVEQLNRTADNAASP
                     AAAAKLYTESLRLQPDNGPALYGLGRALLEQDRAAESLEILRKLDKLTPNHPDTLTAM
                     AAALARQPNLRRAQIAEGLDYATRAVELRSDSPAAWHILSVLHHANGDYAAAAEAAIH
                     SVTLASQQELDPDLIMVYQQQELACFDALAVFSPLE"
     gene            complement(1318..2268)
                     /locus_tag="PKI64_08395"
     CDS             complement(1318..2268)
                     /locus_tag="PKI64_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOE01065.1"
                     /translation="MKTLRKIIDFLNSRNGTRVLSVVVAVVVWHAIKTATSNPLPITD
                     IPLIIQPPPGWSVVDSSAKSVNINFRGTRDDLRYLNRDAIKVSLDGRAHTATNPLTKV
                     LGPADVNAPGNARIENIIPAEVTVRFDRIIKKQVPVRLETLNILPDGFALEKTVITPA
                     TVELTGLAYLLDNVDYISTTPIDLDGRSRSVNKRRSQLVLPETITGIKVDPATVTVDL
                     TITEHFINTTFDGLTILPMLPIGHCPTIDLTPDIANVTVRGRPELMHKLAAEDLRLFV
                     DLTALPANRKSGTLPIRAVLPSGVLLVSTDPDTVKISLKE"
     gene            complement(2252..3100)
                     /gene="cdaA"
                     /locus_tag="PKI64_08400"
     CDS             complement(2252..3100)
                     /gene="cdaA"
                     /locus_tag="PKI64_08400"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOE01066.1"
                     /translation="MGNALWPGTHVLLEIAILSVLFYYTILFFRGTRGSQVLFGFVIA
                     LIILLLLTRNFHLDTLNWLLQQVSVYFVIAFVIIFQPEIRRALAELGKQHVFSVKRER
                     SVLEEVVKAVTHLANGKIGALIAIEREINTLVVQETGVRLDALVSADLLTTIFYPRTT
                     LHDGGVIIEGNRVRAAGCVFPLSARDIGKTGTRHRAAVGLAEDTDAVVIAISEETGAI
                     SLAFRGRLIRGLDEERLRRILTTILARAPRIRSRWQRLRSALDLSAEGLAHTEEMMER
                     EIEDTP"
     gene            3427..3846
                     /gene="msrB"
                     /locus_tag="PKI64_08405"
     CDS             3427..3846
                     /gene="msrB"
                     /locus_tag="PKI64_08405"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOE01067.1"
                     /translation="MLEKVVKTDAEWRQQLTPEQYRVTRQAGTERACSGALYLNREPG
                     EYHCICCDLPLFVSGHKFDSGTGWPSYTQPVAAEHIVEHEDRTWGMVRTEVRCARCDA
                     HLGHVFNDGPPPTGLRYCINSVALKFVPRNPDAHAEQ"
     gene            complement(3851..3934)
                     /locus_tag="PKI64_08410"
     tRNA            complement(3851..3934)
                     /locus_tag="PKI64_08410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3899..3901),aa:Leu,seq:gag)
     gene            complement(4025..4100)
                     /locus_tag="PKI64_08415"
     tRNA            complement(4025..4100)
                     /locus_tag="PKI64_08415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4065..4067),aa:Met,seq:cat)
     gene            4227..5486
                     /locus_tag="PKI64_08420"
     CDS             4227..5486
                     /locus_tag="PKI64_08420"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOE01068.1"
                     /translation="MADLASKPLLDWVENSRAGVSSPCTALTRLLPPGITGIARQLHR
                     QLDGYRMSPLKSLSGLAQLIGVGGVWVKDESQRLSLDSFKVLGGSFAIYQFLRKRLGI
                     RDRELTVTELKSEKTRAQLGDLCFATATDGNHGRGVAWAAKQLGFRCVIYVHERTSRA
                     RIKAIEAFGAQVKVVAGTYDDAVRMVMRDAATNGWQIISDTSWPGYEDIPCWVMQGYT
                     TMMTEIQEQLAGQGIARPTHVFVQAGVGALAAAVAGYYRGLFGDSRPVMTVVEPDRAA
                     CLYESARQGTGQARSVGGDLDTIMAGLACGDPSPIAWEILDHCADFFLSCPDFVAAKG
                     MRVYAVPVSGDPFIVSGESGAVTLAAMMYIVEHESLQDLKRRMKLGPDSQILLINTEG
                     NTDPGHFRRVVWEGSDPVPQEYRFPLV"
     gene            5570..7021
                     /locus_tag="PKI64_08425"
     CDS             5570..7021
                     /locus_tag="PKI64_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOE01069.1"
                     /translation="MRDFHGRILLIGFGIVAQTLLKMLPRHLRVPLNRITVIDFADCT
                     EILRPWTAKGVRYVRERVTAINLARLLSTYVEAGGIIVDLAWSIDCFDIMEWARKNGV
                     LYVNTSLESWDSTGASTQRTTFEKSLYVRYNKVLAAMAAAPRHAPTAVLDHGANPGLI
                     SHFVKRGLLDIGQHILKKNRMTRGSARRLQRLMEDGEFAELARAVGVRVIHCSEWDRQ
                     LSRVPKRHEEFVGTWSIEGMWEEAISPSELGWGTHEKRLPPLAIVPQNGPGNQIILPQ
                     MGMNTLVRSWVPDQEIVGMLVTHGEAFTISHALTVRRRGRVVYRPTVHYAYMPSNDTL
                     ASLHEMRCRNYELQTNKRIITDEISEGDDRMGALLMGPKFQAWWTGSILSAAEARKKV
                     PHSNATAVQVAAGMLAAVLWAIANPDRGIHLPEDLPHEEILRHAAPYLGQLVSQHSTW
                     SPLQRHRVFFAENHEATPDTSDPWQFVNFRFQP"
     gene            7097..7173
                     /locus_tag="PKI64_08430"
     tRNA            7097..7173
                     /locus_tag="PKI64_08430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7131..7133,aa:Pro,seq:tgg)
     gene            complement(8931..9164)
                     /locus_tag="PKI64_08435"
     CDS             complement(8931..9164)
                     /locus_tag="PKI64_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOE01070.1"
                     /translation="MHFELTRLSSRGQIVIPEVTRKVIGLRTGTKFAIFTDGKNILLQ
                     PLAAPDVKGFKKIIAEAEKTKARAQTKAKEGAK"
BASE COUNT         1873 a         2912 c         3064 g         1923 t
ORIGIN      
        1 ctacaatcca aattgatgaa aatgcgctag ggcgctgccg gcacaaagcg catggtgatg
       61 ggggtgcggt aggcggggac gatgttggtg ttgacggcga ggatttcatc gtcggcaccg
      121 caggggaggg ggaggttgat gatggcgtag gggtcccagt agctcaccac gtaggattcc
      181 tcagcgaagg ctttgaagtg gccatcttta atcatggagc cggtgaaggt ccacggggtg
      241 tccggcagaa cagaatcggt ttcgttgttc caaatgaagg cttcggcgcg ggcggtgcgg
      301 gtctcgtcgc cctcctgcca atcaacgaaa atgttaaccg ggtcgccatg cggtgggccc
      361 tcgccgacgg ccggcatggg tgggccggat ttgaggcccg tgaggaggag ggcagcttgc
      421 agatccaatg ggttgagcgg aatgacaaac accgattcat ggaccttgcc ttgaatgccg
      481 caggccaaca cttcgatcag gccggcggtc tggttgaccc agccggtggc ggtgacggtt
      541 ttggtgctgg catcgagcgt gaccgcgcca agagtggcga catggccttg ccacaaaacc
      601 ggcgagggtt caggctgcgg ctcttggccg gcaggataaa tcacaatggg ctgggtggcg
      661 ttggtgtcgg ccgttccggc gggcgggggg acaactgtct gcgcagcggc aaggcgcgcg
      721 ccgattactc caacggcgag aagactgcta aggcatcgaa acatgcgagc tcctgttgtt
      781 gatagaccat gatcagatcc ggatccaatt cttgttgact ggcgagagtg acggaatgaa
      841 tggcggcttc ggcggcggcg gcgtaatcgc cgttggcgtg atgcagcacg gacaagatat
      901 gccaggcagc gggcgagtcc gaacgcaact cgacggcgcg cgtcgcgtaa tccaagcctt
      961 ccgcgatctg ggcgcggcgc aggttgggct ggcgggcgag tgccgcggcc atggccgtga
     1021 gagtgtccgg atggttggga gtcagtttgt caagtttacg cagaatttcg aggctttcag
     1081 ccgcgcgatc ttgttccagc agcgcccggc ccaagccata gagcgcgggg ccgttgtcgg
     1141 gctggaggcg cagagactct gtatacagct tggccgcggc tgcgggggag gcggcattgt
     1201 cggcggtgcg gttgagctgc tcgacctccg gggagagctg tggccattca gatgtttgcg
     1261 cgtcaacgga gccaccgcaa accgccagca gaatggcaat caggccccag cgcattgcta
     1321 ttccttgagg ctgattttga cggtatcggg atcggtgctg accagtaaga cgccgctggg
     1381 caatacggcg cgaatcggca gggtgcccga tttgcggttg gcgggtaatg cggtcaaatc
     1441 aacaaagagg cgcaggtctt ccgctgcgag tttatgcatc agctccgggc ggccgcgaac
     1501 ggtcacgttg gcgatgtccg gggtgagatc aatggttggg caatggccga tgggcagcat
     1561 tggcaggatg gtgaggccat cgaaggtcgt attgatgaaa tgttcggtga tggtgaggtc
     1621 cacggtgacc gtcgccgggt ccaccttgat gccggtgatt gtttccggca ggaccaattg
     1681 ggagcgtcgt ttgttgacgg agcggctgcg cccgtccaga tcaatgggcg tggtgctgat
     1741 gtagtccacg ttatcgagca ggtaggccaa gccggtgagt tccaccgtgg ccggcgtgat
     1801 gacggttttt tccagtgcga agccgtccgg aaggatgttc agcgtttcca gccggacggg
     1861 gacctgcttt ttgatgatcc ggtcaaagcg cacggtgact tcggcgggaa taatgttttc
     1921 gatccgtgcg ttgccgggcg cgttcacatc ggcggggccg agcactttgg tcagcgggtt
     1981 ggttgcggtg tgcgcgcggc cgtccaagct gaccttgatg gcgtcgcggt tgagatagcg
     2041 caggtcatcg cgcgtgccgc ggaaattaat gtttacgctc ttggccgagc tgtccaccac
     2101 gctccagccg ggcggcggct ggatgatcag cgggatatcc gtaatgggca gcggattgct
     2161 ggtggcagtt ttgatggcgt gccaaaccac cacggccacc accacggaaa ggacacgggt
     2221 gccgttgcgt gaattcagaa aatcaatgat tttacggagt gtcttcaatc tcgcgctcca
     2281 tcatttcttc agtatgggcg aggccctcgg cggacaaatc cagcgcgctg cgcaagcgtt
     2341 gccaccggct gcggatgcgc ggggcgcgcg cgaggatcgt ggtcaagatg cggcgcaggc
     2401 gttcttcatc caggccccgg atcaagcggc cccgaaacgc cagcgaaata gcgccggttt
     2461 cttcggaaat ggcaatgacg acggcgtcgg tatcctcggc cagccccacg gcggcccggt
     2521 ggcgggtgcc ggttttgccg atgtcgcgcg cggagagcgg aaatacacaa ccggccgccc
     2581 gcacccggtt gccctcaatg atcacgccgc catcgtgcag cgtggtgcgg ggataaaaaa
     2641 tcgtggtgag cagatcggcg gaaaccaggg catcgaggcg cacgccggtt tcctgcacga
     2701 ccagggtgtt gatttcgcgc tcaatggcaa tcaacgcgcc aattttgccg ttggccaaat
     2761 gagtgaccgc cttgacaact tcctccagca cgctgcgttc gcgcttcacg gaaaacacgt
     2821 gctgtttgcc caattcagcc agcgcgcggc ggatttccgg ctggaaaatg atgacgaatg
     2881 caatgacgaa atagacggac acctgctgca gcagccaatt caacgtatcc aagtggaagt
     2941 tgcgggtcag caacaggagt atgatcaggg cgataacaaa cccgaacaaa acctgcgagc
     3001 cgcgcgtccc ccggaagaac aggatggtgt agtaaaacaa caccgataaa atggcaattt
     3061 ccaggagcac gtgtgtaccc ggccacaggg cgttgcccat cgcttttaac caatcccaat
     3121 tcacgcaaag caatctgcca tgcgtcgaga actggcgcaa gttccaaagc aaatggtgcg
     3181 ccgcgcggct tgacgtgcgg ctggctcacg gccagcgcgg ggccaaagcc ttcacgcgca
     3241 gcggcgtgaa caatcgcata acattcgccg tgcgccgccc cgtgtggtac actggtacaa
     3301 gtgaaccgga gcagcagaaa gagtggtttg gcagccgggc ggctggcgct gctgtgtacg
     3361 ttggtcagcg gcgtggcgct cctggcttct gcgcggccgg cggtggcgga acaaagggaa
     3421 cggaacatgt tggaaaaagt ggtcaagact gatgcggagt ggcggcagca acttacgccg
     3481 gaacaatacc gggtgacccg gcaggccggc acggaacgcg cctgttccgg ggcgctgtat
     3541 ctgaaccggg agccgggcga ataccactgc atctgttgcg acttgccgct ttttgtttcg
     3601 ggccataagt tcgattctgg taccggctgg cccagctata cccagccggt ggctgcagag
     3661 catattgtgg agcacgaaga ccgcacttgg ggaatggtgc gcacggaagt gcgctgtgcc
     3721 cggtgcgatg cccatttggg ccatgtcttc aacgatggcc ccccgcccac gggcctgcgc
     3781 tattgcatca attcggtcgc gctgaagttt gtgccgcgga atcccgacgc tcacgccgaa
     3841 caataaaaaa tggtgctcgg gacaggactt gaacctgcac ggcttgcgcc acatgcccct
     3901 caaacatgcg tgtctgccat tccaccaccc gagcaccgaa ctcaaccatg agggtttcgt
     3961 gagttggcgc ccgacgatac ccatccggtt tctcccccgc aagcattttg cgccggcagg
     4021 gaagtggtgg cgggaaccgg ggtcgaaccg gtgacctaag gcttatgagt cctccgctct
     4081 gccaactgag ctatcccgcc aaagggattg attgaatcgc ggggaatgta cggaactggc
     4141 ccgcggatgt caaccagccc gctttgcgct tgataataat ttcgttctgg cgaagatgcg
     4201 gtattcgcat gaataaggag aacgtcatgg ctgacctcgc gtcgaaacct ttgctggatt
     4261 gggtggagaa ttcccgggcc ggagtgtcca gcccctgcac ggcgctgacg cggttgttgc
     4321 caccgggcat caccggcatt gcccgccagt tgcaccgcca attggacggc taccggatga
     4381 gcccgctgaa aagtttgtcc ggcctggcgc agttgatcgg cgtgggcggg gtttgggtga
     4441 aggatgaatc gcagcgcttg agcctcgact cattcaaggt gctgggcggg tcattcgcca
     4501 tctaccaatt tttgcgcaag cggctcggca ttcgcgaccg cgagctgacc gtgacggagc
     4561 ttaaatcgga aaaaacccgc gcgcagttgg gcgacctgtg cttcgccacc gccaccgacg
     4621 gcaaccatgg ccgcggggtg gcgtgggcgg ccaagcagtt gggtttccgc tgcgtgatct
     4681 atgtgcacga gcgcacctcg cgggcgcgga tcaaagccat cgaggcgttt ggcgcgcagg
     4741 tcaaggtggt ggccggcacc tatgacgacg ccgtgcgcat ggtcatgcgc gatgccgcaa
     4801 cgaatggctg gcagatcatt tccgacacct catggccggg ctacgaagac attccttgct
     4861 gggtcatgca gggctacacc accatgatga cggaaatcca ggagcaactg gccgggcagg
     4921 ggatcgcgcg gcccacgcac gtgtttgtgc aagccggggt gggggcgctg gccgccgccg
     4981 tggccggtta ttaccgcggg ttgtttggcg attcgcgccc ggtcatgacg gtggtggagc
     5041 cggaccgcgc ggcttgcctc tatgaatcgg cgcgccaagg cacgggccaa gcccgctccg
     5101 tcggcggcga tctggatacc atcatggccg gcctcgcctg tggcgacccc agccccatcg
     5161 cgtgggagat tttggaccat tgtgcggact tctttttgtc gtgcccggat tttgtggcgg
     5221 ccaagggaat gcgggtatat gcggtgcccg tgtcgggcga tccgttcatc gtgtccggcg
     5281 aatcgggcgc ggtcaccttg gcggccatga tgtacattgt cgagcacgag tcgctgcagg
     5341 acctgaaacg ccggatgaaa ctgggtccgg actcgcagat cctgctgatc aacaccgaag
     5401 gcaacaccga ccccggccac ttccggcgcg tcgtgtggga gggcagcgat cccgtgccgc
     5461 aagaatatcg tttccctttg gtgtagtgcg gttgcaatcc gccaattcag ggaggtggcc
     5521 cattcggcaa tggccggccc cgcgccggcc ccaaatcagg agataaaaca tgagagattt
     5581 tcacggacgc attttgctta tcggctttgg cattgtggcg caaaccctgc tcaaaatgct
     5641 gccgcgccat ctgcgcgtgc cgctgaaccg catcacggtc attgactttg ccgattgcac
     5701 cgaaatcctg cggccatgga ccgccaaggg cgtgcgctat gtccgcgagc gcgtcaccgc
     5761 catcaatctg gcccggctgc tgtccactta tgtcgaagcc ggcgggatca ttgtggattt
     5821 ggcatggagc attgactgct tcgatatcat ggaatgggcg cgcaagaacg gcgtgctcta
     5881 cgtcaacacc tcgctggagt cctgggacag caccggcgcc agcacgcaac gcaccacttt
     5941 cgaaaagtcc ctgtatgtgc gctacaacaa ggtactggcc gccatggccg ccgccccgcg
     6001 ccacgccccc acagccgttc tggaccacgg cgccaacccc ggcctgattt cgcattttgt
     6061 gaagcgcggc ttgctggata ttggccagca catcctgaag aagaaccgca tgacccgcgg
     6121 cagcgcccgg cgcttgcagc gcctgatgga agatggcgaa ttcgccgagc tggcccgggc
     6181 ggtcggcgtg cgggtgatcc attgcagcga gtgggatcgg cagctcagcc gcgtacccaa
     6241 gcgccacgaa gagtttgtcg gcacatggag catcgaggga atgtgggaag aagccatctc
     6301 gcccagcgag ctggggtggg gtacccatga gaaacgcctg cccccgctgg ccattgtgcc
     6361 gcaaaacggc cccggcaacc aaatcatcct gccacagatg ggcatgaata cgctggttcg
     6421 ctcgtgggtg ccggatcagg agatcgtggg catgctggtc acgcacggcg aagccttcac
     6481 catttcccac gccttgaccg tgcgccggcg cgggcgggtg gtgtaccgcc ccacggtgca
     6541 ctacgcctat atgccttcca acgatacctt ggcctccttg catgaaatgc gctgccgcaa
     6601 ttatgagttg caaaccaaca agcggatcat caccgacgaa atcagcgaag gtgacgaccg
     6661 gatgggcgcg ctgctcatgg gacccaagtt ccaggcgtgg tggacgggct ccatcctttc
     6721 cgcagcggaa gcccgcaaga aagtgccgca ttccaacgcc acggccgtgc aagtggccgc
     6781 gggaatgctg gccgccgtgc tgtgggccat tgccaaccct gaccgcggca ttcacttgcc
     6841 cgaggacctg ccgcacgagg aaatcttgcg ccatgccgca ccctatctgg gccagctggt
     6901 ctcccagcat tccacttggt cgcccctgca acgccaccgt gttttctttg ccgaaaatca
     6961 cgaggccacc cctgacacca gcgatccgtg gcagttcgtc aacttccgtt tccaaccctg
     7021 atccggactg tgttcggatt ccggcggttt tgctttgacg gcacgcggct tttgggcaaa
     7081 agtgcccgcc gattgacggg gcgtggctca gcctggtaga gcgcaaggtt tgggaccttg
     7141 atgtcgcggg ttcaaatccc gccgccccga ccactttaga ttcggctttt tttcggccca
     7201 gtttttccaa gggccgacaa actccacgca cagacgcggc gggaggtttc ccctattgct
     7261 caccctttgc gatgaccact ttttcccggc ggacagatcg ggggcggcgg ctacgccgtt
     7321 ggtttggcaa gggtgttcag cggcggcgcg acgagcgcgg gaggcgcgaa ctttcaggct
     7381 ggggtacaag gatttgcggc acggatcggc ggaagcgatg cgcgggggtt cctgcggggt
     7441 cgggaggtcg gcgcgtgcgg caaggccgcc gcgccgagcg gctgggtcgg atttctggcc
     7501 cgaccgttcc gaaggggggt gcatccggct ttcgtcctcg ttttccccgg cttccgggcg
     7561 ctctaaaaag aggttcaatt ggaaccgctg gaaaaattcg cgcaattccg catagctgcc
     7621 cgaaatcgcc gtgttttggg ccagattcgc ggctttcaca aaatcggcca gaggttcaat
     7681 ccagcagttg ccttgagact tgatccggtc gattttctcc cgtaggcggg tcttctcggt
     7741 aaaatattcg gctttgcggg cggcgtactc ctcccgcgtg atagacccat cgaggaatac
     7801 atccagcaga cgcgccacct tcgcatcgat ctccgccagc cgcttttgat gggccgaagc
     7861 ctccgccgtc gcgcggtccg ccccatcgcg ccgccattcc tcgatccgcc ccagcatcaa
     7921 ctccgcccac tcgtcggata tccccacgcg caggagttcc cgccgcatct gctgggcaag
     7981 gtattcctcg cggatgtacc caaccaggtc gcaggaaccg agtttcttgg cgcaacggta
     8041 atagtgatag gtcttgtgcc gctccaccgt cacccaggac ccgcacaccc ggcaccgcac
     8101 gaagcccagg aacgccaccg gctgcaaccc cttcttggtg cggtagcgcc cccggccgcg
     8161 ctccagcaag acccgctgga cgcgctcgaa aagctccacg gggataatgc gctcgtgtgt
     8221 gccctcgtac tcgtcgcccg cccacgcgaa aaacccgatg tagaaccgat ccgaaagaat
     8281 ctgcggaatc acgctgcggg cgatgggctt ttcctgatgg ctcaaaagcc cccactgcgt
     8341 agccaattcc accatttggt cgtagttgta gcggcctgtc gcataggtct cgaacaatcg
     8401 ccggaccatc ggggctttct gctggtgcac ttcgatggtc cgcaagcgcg gttcgttcac
     8461 atagcccaaa ggcggtttgt gcggatagac gccccgccga atcttctccc gcataccccg
     8521 ccgcacattc tccgaaagcg catccacata atatttcgac tgtccgaacg ccatattgag
     8581 gacgaatttc ccctgcggcg tgttctcaaa ccagaatgtc gggaacttca acgccgtgat
     8641 tttccccgtg tccaccagcc agatgatttt tccgccgtcc acgctgttgc gggcgaggcg
     8701 gtctggatgc cacgccaaca gcgcatccgc ttcgcccttc tccaccctta aaagcatgtc
     8761 gttgaagatc ggtcgccccg gctccttcgc cgtcatggac tcgacaaatt cgcggacaat
     8821 caccagcccc tgtttctttg cgtactcgcg caattcgttt cgctgcgcat caatcgaaag
     8881 aatctgccgc tcttcatcgt ccgtgctttt ccgcgcgtat aggaatactt tcattttgcc
     8941 ccctccttgg ccttggtttg cgccctggcc tttgttttct ctgcttctgc gatgattttc
     9001 ttgaagccct tcacatccgg cgcggcaagc ggttgcaaga ggatgttttt accatccgtg
     9061 aaaatggcga acttcgtacc cgtgcgcaga ccaatcacct tccgcgtcac ctctggaatc
     9121 acaatctgcc cccgcgaaga aagccgggtc aattcaaagt gcattcgcag cctccattta
     9181 taagatgatc ttatatattc actttacccg aggaaggaaa cgctggcaag gcgattcctg
     9241 gaaataatct aaatttaacg cggacgggat cgaaccgctc ctgcccgcgt gcgaagaatg
     9301 agcgccaaag ggcaggaagt ggggcggcct ggccgctacc gacggcggca agcgaagcgc
     9361 gcagggatgc accccccttc ggaacggtcg ggccagaaat ccgacccagc cgctcggcgc
     9421 ggcggccttg ccgcacgcgc cgacctcccg accccgcagg aacccccgcg catcgcttcc
     9481 gccgatccgt gccgcaaatc cttgtacccc agcctgaaag ttcgcgcctc ccgcgctcgt
     9541 cgcgccgccg ctgaacaccc ttgccaaacc aacggcgtag ccgccgcccc cgatctgtcc
     9601 gccgggaaaa agtggtcatc gcaaagggtg agcaataggg gaaacctccc gccgcgtctg
     9661 tgcgtggagt ttgtcggccc ttggaaaaac tgggccgaaa aaaagccgaa tctaaagtgg
     9721 tcgagcttac tggacagcca aagaaactgt tttgcgtagt ttccccctct gc
//
LOCUS       DAOPNP010000093         9593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8045_length_9593_cov_8.920843, whole genome shotgun sequence.
ACCESSION   DAOPNP010000093 DAOPNP010000000
VERSION     DAOPNP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_9593_cov_8.920843"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKI64_08440"
     CDS             complement(<1..865)
                     /locus_tag="PKI64_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOE01071.1"
                     /translation="MQKAYIDYSMSVIIGRALPDARDGLKPGNRRILFAMREGNYTHS
                     RPYVKCARVVGDVIGKYHPHGDAAVYDTLVRMAQDFSTRYLLIDGQGNFGSIDGDPAA
                     AYRYTECRLKRTAEEMLADIDKDTVEMQPNYDEKLLEPKVLPARIPNLLVNGSTGIAV
                     GMATNIPPHNLGEIIDGTVHLIDNPTATVADLMQFVKGPDFPTGGTLYGLAPIREMYL
                     TGRGHLRLRGKATVEPWKADRERIIITEIPYAVNKATLIEKIADLVHEKKIEGISDLR
                     DESDKDGIRVVV"
     gene            complement(1030..3480)
                     /gene="gyrB"
                     /locus_tag="PKI64_08445"
     CDS             complement(1030..3480)
                     /gene="gyrB"
                     /locus_tag="PKI64_08445"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOE01072.1"
                     /translation="MSEEELKPDTGAAPVAKPEAYDGSKIQVLEGLEAVRKRPAMYIG
                     DTFQRGLHHCVYEVVDNSIDEALAGYCTRILVHLAADGSVSVSDNGRGIPVDPHPKLK
                     KPAVEVALTVLHAGGKFDHGSYKVSGGLHGVGVSCVNALSEWMEVEVRRNGKIYRMRF
                     ERGVTKSPLEIIGSCTDQGTKVTWYPDDQIFSTLEFSWDILAARLRELAFLNKGIEIV
                     FQQEQHDGAMRTETFRYDGGIREFIQHLNKNKVTLHPEVIYFEKEQDGVAVEIAMQYN
                     DGYAENVLSYANNINTIEGGTHLTGFRGALTRVVNTYGRNNKLLKEEDTMSGEDIREG
                     LVAVVSVKVPDPQFEGQTKTKLGNSEVEGIVSSIVNEQLANFFEETPAVARKVVEKGV
                     LAARAREAARKARDLTRRKGALDSGSLPGKLADCSERDPALCELFLVEGDSAGGSAKQ
                     GRDRRFQAILPLRGKILNVEKARLDRMLNNQEIRTMITAIGAGIGHDEFDVAKVRYHK
                     IIIMTDADVDGSHIRTLLLTFFYRQMPQLIERGYVYIAQPPLYKIKRKKQERYIDNDR
                     HLSRVLIELGIEDVRMLDLAGGTVLDDGKMAEVLKILEEIEHLAGSLARKSVDFDEYV
                     KRYDSAQNRLPLYRVRVIPPGGDIENQEIHLAFSDEEMRKIREDAEQRMGPLDPAQFK
                     WDELHQAPGLVKQFAQLQARGLGIDALLRSDNPIYEAEVDGKKVSLHALGDLLNLVRE
                     VGRRGLSIQRYKGLGEMNPEQLWETTLEPTKRKLLQVQLDDAVRADQTFTILMGDEVE
                     PRRAFIEENALNVRNLDV"
     gene            complement(4056..5813)
                     /locus_tag="PKI64_08450"
     CDS             complement(4056..5813)
                     /locus_tag="PKI64_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE01073.1"
                     /translation="MRKLAWWLAVWVVGQAALAEEMFPRPGWHEAPNPLADEWAVKGG
                     QLTVYAGPYPKSFNYYLDQNVFSAELFGQLYETLLTENPVTLELEPLLAARCTLGDDR
                     KTFTFYLDPRARWSDGQPVTAEDVRWTFDAVMDPRHLTGPHKIGLGRFAPPEVLDERT
                     IRFVANEVHWVNLLVLAGLQVLPKHAWAGQDFNLQNFEFPVVSGPYRLGVIKEGLYAN
                     LERRADWWAAGLPRFQHVANFATLRYRFFEEAENAFEAFKKGEIDLCPIYAAHRWANQ
                     TSGEKYDRNWIVKQEVVNLNPVGFQGIALNMRRPPLDDPRVRRALAHLFNREKMNATL
                     MFNAYFLHKSYYETLYDDAHPCPNEEFHFDKERARALLAEAGWRADPTTGLLMKDGRP
                     LRLQYLSRGGTDDKFIAIFQEDLKDVGISLSVDKKDWAAWAKDMDHYNYDMTWAAWSG
                     GLYPDPESMWLSSEADRPAGQNITGYKNPAVDELIIAQRAEFDVQKRAGLVRQIDGLV
                     TRDVPYILLWNLNYTRLLWWNKFGMPDAPLGKYGSERAAASYWWYDPDAVADLQHAQA
                     LGRALPPRPARSTYSDPEP"
     gene            5917..7785
                     /locus_tag="PKI64_08455"
     CDS             5917..7785
                     /locus_tag="PKI64_08455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE01074.1"
                     /translation="MNTNSPALDSASPQPDDLVAARYEIRRSLGSGGSGTVFLAWDVQ
                     LKRQVAIKRWNPLEPMLDDPAGTERLWREAMTLAAIQHPNILTIHDFGVDAKGPYVVM
                     EFIDGETLNQAVHRAPFSLEEFTEAAQQVLEALAAAHQAGMIHRDLKPQNIMRMRLPS
                     GAWQYKVLDFGLARFVQQPTVQSMEGNTSIYGSILYIAPEQLRHLPLDARTDIYAIGC
                     VFYYLLSGQNAIIGDTIPALITSRLENRVKSLAEWRPDLPKALCDWVMKAMAYDPDDR
                     FPSAVAALGALRKILPNTGRPSSIRVTSTPAGAAGIRIMTPPPAAHKTGPLTPTASFA
                     IPTAPLPAGGGIAATAPLPAAPPAPRASRQWPLVAAVSGLALLVAFALFLHFRPARHA
                     AEPEPPTAAATTAPQAPVVVSWRDESMPVEDKISSWPASIAEARRPLIQKYFEQLRDL
                     QDPKWGSIGTVLECLAAIQYNSRHDPKKTKALCNFTYHDETGRTLGEIDVVIWNVQEN
                     RAEVMYEAAVSDQLHRKAVPSRNQLQRFPDSIRAGKVAKVLYPYDSSLHFEPSQFVDA
                     KVCIMGNQGALAAGFDYEVDITRFENDYLQRRLIDWRKAHQPAKKKKGAPPSEPAP"
     gene            7782..8291
                     /locus_tag="PKI64_08460"
     CDS             7782..8291
                     /locus_tag="PKI64_08460"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOE01075.1"
                     /translation="MKHAHARRADFDFRWPEPPLNVVLVSPEIPPNTGNIARLCAATG
                     TRLHLVEPLGFQITNAQLRRAGLDYWDDVNVRVHASWPAFLAAAQPDRLFLYSTGSAR
                     RYDRVAYRPGDTLVFGCETKGLPDELLAAHAESVCGIPIQTRHVRSLNLSSAVAVVVY
                     EALRQLNAP"
     gene            complement(8298..8750)
                     /gene="dtd"
                     /locus_tag="PKI64_08465"
     CDS             complement(8298..8750)
                     /gene="dtd"
                     /locus_tag="PKI64_08465"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOE01076.1"
                     /translation="MRAVVQRVARAEVRSEGRVTGRIGPGAVVLLGVGRTDTAAEAAK
                     LADKVAKLRIFDDADGKMNLSIAAVPDGKFIVVSQFTLLGNTERGNRPSYIEAAPPEQ
                     AEALYELFVAHLRGLGFGVETGVFRTMMQVELVNDGPVTLLLDTADWA"
     gene            complement(8757..9464)
                     /locus_tag="PKI64_08470"
     CDS             complement(8757..9464)
                     /locus_tag="PKI64_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01077.1"
                     /translation="MASEDLLLRCDQCGEMNRLPSVYCKKCGTKLNFAKAEQKMMTAD
                     RPTLAQQTRTLVRLAVALGMLVVVLLTIWPARMVRTVGEELDAKRYRLKGELLIDALN
                     REVPASQVIEEKEINAYLRGVVAAQPAAHGVLAAALQDAGARFFPGRAEIYLAISRGP
                     LTFTSLFYVQVKNDQLVVTGAKLGHLPLPGILGRAYANTQINLFRQFRNEARIVRHLS
                     GALIQSETIELLVGPVQ"
BASE COUNT         1761 a         3116 c         2753 g         1963 t
ORIGIN      
        1 ccaccaccac gcggatgccg tccttgtcgg actcgtcgcg caggtcgctg atgccttcga
       61 ttttcttctc atggaccaaa tcggcgattt tttcgatcag ggtggccttg tttaccgcgt
      121 aagggatttc ggtgatgatg atgcgctcac ggtcggcttt ccacggctcc acggtggcct
      181 tgccgcgcag gcgcagatgg ccgcggccag tcaggtacat ctcgcggatt ggcgccaggc
      241 cgtagagcgt gccgccagtc ggaaaatccg gccccttcac aaactgcatc agatcagcaa
      301 ccgttgccgt tgggttgtca atcaggtgga cggtgccatc aatgatttcg cccaggttgt
      361 gcggcgggat attggtggcc atgccgaccg cgatgccggt ggagccattg accagcaggt
      421 tgggaatccg cgcgggcaag actttgggct ccagcagctt ttcgtcgtaa ttcggctgca
      481 tttcgacggt gtctttgtca atgtccgcca gcatttcttc agcagtgcgc ttgaggcggc
      541 actcggtata ccggtaggcg gcggcgggat cgccatcaat gctgccgaag ttgccctgcc
      601 cgtcaatcag caggtagcgc gtggaaaaat cctgcgccat gcgcacgagg gtgtcataga
      661 ccgcggcgtc gccgtgggga tggtatttac cgatgacatc acccaccacg cgcgcgcatt
      721 tgacgtaagg ccgcgagtgg gtgtagttgc cctcgcgcat ggcaaacagg atgcgtcggt
      781 tgccgggctt gaggccgtca cgagcgtcgg gcaacgcccg gccaatgatg accgacatcg
      841 agtaatcaat gtaggccttc tgcatctcat cttcgatgtt gatgcgttcg atgcggtccg
      901 gcgcgttttg cggcgcggcg gcggcgcggg tttcgccggc gaactcatcg ccgggcgcgg
      961 gggtgacttc atcgctcatg gactatcctt tttcggtcaa ctttcggcac ggtggcgttc
     1021 ccgccgcgac tacacatcca aattccgcac attgagggcg ttttcctcga tgaatgcgcg
     1081 gcggggttca acttcatcgc ccatcaggat ggtaaaggtc tggtcggcgc gcacggcgtc
     1141 gtccagttgc acttgcagca gtttacgctt ggtgggttcg agggtagttt cccaaagctg
     1201 ttcgggattc atttcgccca agcccttgta gcgctggatg cttaggccgc ggcggcccac
     1261 ctcgcgcacc aggttgagca aatcgcccaa cgcgtggagc gacacttttt tgccgtccac
     1321 ctcggcttca tagatggggt tgtcgctgcg cagcagcgcg tcaatgccca gcccgcgcgc
     1381 ctgcaactga gcaaactgct tgaccaggcc gggggcctga tgcaactcat cccacttgaa
     1441 ctgggcaggg tccaacggcc ccatgcgttg ctccgcgtcc tcgcggattt tgcgcatttc
     1501 ttcatcgctg aacgccagat ggatttcctg attttcgatg tcgccgcccg gcggaatcac
     1561 ccgcacgcgg tagagcggca agcggttctg cgcggagtca tagcgcttca catactcgtc
     1621 aaaatccacg ctcttgcgcg cgagagaacc ggccaaatgc tcgatttctt cgaggatttt
     1681 gaggacttcg gccattttac cgtcgtccag cactgtgccc ccggcgaggt cgagcatccg
     1741 cacatcttcg atgcccagct cgatcagcac gcgcgagaga tggcgatcgt tgtcaatgta
     1801 gcgctcctgt ttcttgcgct tgattttgta gagcggcggc tgcgcaatgt acacatagcc
     1861 gcgctcgatc aactgcggca tctggcggta gaaaaacgtc agcagcagcg tgcgaatgtg
     1921 cgagccgtcc acgtcggcat cggtcataat gatgattttg tgatagcgca ctttggccac
     1981 atcgaactcg tcatggccga tgcctgcgcc gatcgcggtg atcatggtgc ggatttcctg
     2041 attattgagc atccggtcca gccgcgcctt ttccacgttg aggattttgc cgcgcagcgg
     2101 caggatggct tggaagcggc ggtcgcgccc ctgcttggca gagccgccgg cggaatcgcc
     2161 ttccaccaga aacagctcgc atagcgccgg gtcgcgttcg gagcaatctg ccagcttgcc
     2221 gggcaacgat cccgaatcca gcgccccttt gcggcgggtc aggtcgcgcg ccttgcgcgc
     2281 ggcttcgcgg gcgcgggcgg ccagcacgcc tttttccacc accttgcggg ccactgccgg
     2341 cgtttcctcg aaaaagtttg ccaactgctc attgacgatg gacgacacaa tgccctcgac
     2401 ctcgctgttg cccagcttgg tcttggtttg gccctcaaac tgcggatcgg gcaccttcac
     2461 gctcaccacg gccacaaggc cctcgcggat atcctcgccg gacatggtgt cttcttcctt
     2521 cagcagcttg ttgttgcggc cgtaggtatt taccacgcgc gtcaacgcgc cacggaagcc
     2581 ggtcaggtgc gtgccgccct cgatggtgtt gatgttgttg gcgtagctca acacattctc
     2641 ggcgtagccg tcgttgtact gcatggcaat ttcgaccgcc acgccatcct gctccttctc
     2701 gaaataaatc acctcgggat gcagggtcac cttgttcttg ttgaggtgct gaatgaactc
     2761 gcggatgccg ccgtcatagc ggaaggtttc ggtgcgcatg gcgccatcat gctgttcctg
     2821 ctggaacacg atctcgatgc ccttgttcag gaacgccagc tcgcgcaggc gcgccgccag
     2881 aatgtcccac gagaactcca gcgtggagaa aatctggtcg tcgggatacc acgtcacctt
     2941 ggtgccctga tcggtgcagg agccgatgat ctccagcggc gacttcgtca cgccgcgctc
     3001 gaagcgcatc cggtagatct tgccgttgcg ccggacttcc acttccatcc attcgctcag
     3061 cgcgttcacg cagctcacgc ccacgccgtg caagccgccg gagaccttgt agctgccgtg
     3121 atcgaacttg ccgccggcgt gcagcaccgt cagcgccact tccacggcgg gctttttcag
     3181 cttcgggtgc gggtccacgg gaatgccgcg cccgttgtcg gacacggaaa ccgagccatc
     3241 cgcggccaga tgaaccagaa tgcgcgtaca atagccggcc agcgcttcat caatggagtt
     3301 gtccaccact tcgtacacgc agtggtgcag gccgcgttga aacgtatcgc caatatacat
     3361 cgccgggcgc ttgcgcacgg cctccagccc ttccaagacc tggattttcg acccatcata
     3421 cgcttcgggc ttggcgactg gggctgcccc ggtatccggc ttcaattctt cttctgacat
     3481 aaatctaatt tctctctatt caaataatag gaacagtcta ccaaaccagt gcgcaaacgc
     3541 aataaaaacc taaccaaacc ccgccgattt ctcgacgagt aaaccgtttc aattcaaaac
     3601 cgtttttgag ggcctaacgg cctattaact agtttatgac tggaatatga ggttttcctc
     3661 tctatcgggg tgctttcgag tagaacccat atcaatcgct gttttcagtg gcttgtagtc
     3721 gggtttggca tgcggaaaca accgttgtca gccaccttct ttttcccatt tgaaccatgc
     3781 tgtccagagt ttataaactc tggacagttt ttatattatt catatcccaa tcatatacaa
     3841 tttgttatgt atatattttg ggtatttttt aatcgtccat tttcagggga ttgtccacac
     3901 tttataaact ctgggcatat ttaaggtgcg tgatttttgt gtttttatct gcgcggttcg
     3961 gtcctgcatg gcagttgccg accgcaataa ccccactaaa atcagcaaat aacccctccc
     4021 tcccccgcgc ccttggcccg tggggcgtgt gggtatcagg gttcgggatc ggaataggtg
     4081 ctgcgggcag ggcgcggcgg gagggcacga ccgagagcct gagcgtgttg caggtcggca
     4141 acggcatcgg ggtcatacca ccaataactc gcggcggcgc gttcgctgcc atacttgccc
     4201 agcggcgcat cgggcatgcc aaacttgttc caccagagca gccgggtgta gttcaggttc
     4261 cacagcaaaa tgtagggcac atcgcgcgtg acgagcccgt caatctgccg cactaacccg
     4321 gcgcgctttt gcacgtcaaa ttcggcgcgc tgcgcaatga tcagctcatc caccgccgga
     4381 ttcttgtagc cggtgatgtt ctgcccggcg gggcggtcgg cctcgctcga aagccacatg
     4441 ctttccggat cgggataaag ccccccactc cacgcggccc aagtcatgtc gtagttgtag
     4501 tgatccatgt ccttggccca cgccgcccaa tcttttttgt ccacgctcaa ggaaatgccg
     4561 acatccttca agtcttcctg aaagatggcg ataaacttgt catcggtgcc gccacgcgag
     4621 agatattgca ggcgcagcgg ccggccatcc ttcatcagca acccggtggt tggatcagca
     4681 cgccagccgg cttcggccag caaggcgcgg gcgcgttcct tgtcaaaatg aaactcctcg
     4741 ttggggcacg ggtgcgcgtc gtcgtagagg gtttcatagt aggacttgtg caaaaagtag
     4801 gcgttgaaca tcagcgtggc gttcattttt tcgcggttaa acaagtgggc cagcgcgcgg
     4861 cgcacacgcg gatcatccag cggcgggcgg cgcatgttga gcgcaatgcc ctggaaaccc
     4921 accggattga ggttgaccac ctcctgcttc acgatccagt tgcgatcgta tttttcgcca
     4981 ctggtctggt tggcccagcg gtgcgccgca tagatggggc ataggtcaat ctcgcccttt
     5041 ttgaacgcct caaacgcgtt ctccgcttct tcgaaaaacc ggtagcggag cgtcgcgaag
     5101 ttggccacgt gttggaagcg cggcagcccc gccgcccacc aatcggcgcg gcgctcgagg
     5161 ttggcgtaga ggccctcctt gataacgccc agccggtacg ggcctgatac cacgggaaac
     5221 tcaaagttct gcaaattgaa atcctgcccc gcccacgcgt gtttgggcag cacctgcaaa
     5281 ccggccagca ccagcagatt cacccagtgc acctcgttgg ccacaaaccg gatggtacgc
     5341 tcatccagca cctccggcgg cgcaaaccgg cccagaccga ttttatgcgg gccggtcagg
     5401 tggcgcgggt ccatcacggc atcgaacgtc cagcgcacat cctcggcggt gacgggttgg
     5461 ccatcgctcc aacgcgcgcg cgggtccaga taaaaggtga aagtcttgcg gtcgtcaccc
     5521 agcgtgcacc gggcggccag cagcggttcc aattccagcg tcaccggatt ttcggtcagc
     5581 agcgtctcgt acaactggcc aaacaactcg gcggaaaaca cattttggtc gaggtaatag
     5641 ttgaaactct tggggtatgg cccggcatac accgtcaact ggccgccctt cacggcccac
     5701 tcgtccgcca acgggttcgg cgcctcgtgc caccccggac gcggaaacat ctcttcggcc
     5761 agtgccgcct ggcccaccac ccaaaccgcc agccaccatg ccaattttct catgcgggca
     5821 ttatccgcaa ataccgcccg caggaaagcc ggaaagtccg tgtcgccgcc tcgcacctcg
     5881 acaaaacgcg ggcggctctc tatactcccc ggcgtgatga atacgaatag cccggccctc
     5941 gattccgcca gcccgcaacc ggatgacttg gtcgccgccc gctatgaaat ccgccgctcg
     6001 ctcggctccg gcggttccgg caccgtcttc ctggcgtggg acgtgcaatt aaagcggcag
     6061 gtcgccatca agcgctggaa tccgctggag cccatgcttg acgatcccgc cggcaccgaa
     6121 cgcctctggc gcgaagccat gacgctggcc gccatccagc atcccaacat cctcaccatc
     6181 cacgattttg gcgtggacgc gaagggcccc tacgtcgtca tggagttcat tgacggcgag
     6241 acgctcaacc aagccgtaca tcgcgcaccg ttcagtttgg aagaatttac ggaagccgcc
     6301 caacaggtgt tggaggcgct ggccgccgcg catcaggccg gcatgatcca ccgcgatctc
     6361 aagccgcaaa acatcatgcg catgcggctg cccagcggcg cgtggcaata caaggtgctg
     6421 gattttggcc tcgcgcggtt tgtccaacag cccacggtgc agagcatgga aggcaacacg
     6481 tccatctatg gctccatcct ctacatcgcg cctgagcagt tgcgccacct gccgctcgac
     6541 gcgcgcacgg acatctacgc catcggctgc gtgttctact acctgctctc cggccagaat
     6601 gccatcatcg gcgacaccat ccccgcgctg atcaccagcc gactggaaaa tcgcgtcaaa
     6661 tcgctggccg aatggcggcc cgacctgccc aaggcgctgt gcgattgggt catgaaggcc
     6721 atggcctacg atcccgatga ccgcttccct tccgctgttg ctgcgctggg cgcactgcgg
     6781 aaaattttgc ccaacaccgg ccggccatcc tccatccgcg tcacatccac gcccgccggc
     6841 gcggccggca tccggatcat gacgccgccg cctgcggcgc acaaaaccgg accgcttacg
     6901 ccaacggcct catttgccat ccctaccgcg ccattgcctg ctggcggcgg aattgccgcc
     6961 accgcaccac tccccgccgc cccccccgcc ccgcgcgcgt cgcgccaatg gcccctcgtg
     7021 gccgccgtta gcggtttggc gttgctggtg gcgtttgccc tcttcttaca tttccgcccc
     7081 gcgcgccacg cggccgaacc cgagccgccc accgcggcgg ccacgaccgc cccgcaggcg
     7141 cccgtagtcg tgtcctggcg tgacgaatcc atgcccgtcg aagacaaaat ttcgtcatgg
     7201 ccggcctcca tcgccgaagc gcgccgcccg ctgatccaga aatactttga gcagttgcgc
     7261 gatttgcaag accccaagtg gggcagcatc ggcaccgtgc tcgaatgcct tgcggccatc
     7321 cagtacaact cacggcacga tcccaagaaa accaaggcgc tgtgcaattt cacctatcac
     7381 gacgaaaccg gtcgcacact gggcgaaatt gacgtggtga tttggaacgt gcaggaaaat
     7441 cgcgccgagg tcatgtatga agccgcggtg tccgaccaac tccaccgcaa ggccgtgccc
     7501 agccgcaacc agctccagcg cttcccggat tccatccgcg ccggcaaagt cgccaaggtg
     7561 ctctatcctt acgactcttc gctgcatttt gaaccgtcgc aatttgtgga cgccaaggtg
     7621 tgcatcatgg gcaaccaggg cgcactggct gccggattcg attacgaagt ggatatcacc
     7681 cgcttcgaga acgactatct ccaacgccgg ctgattgact ggcgcaaggc gcaccagccc
     7741 gccaaaaaga aaaaaggcgc gccgccttcc gaaccggctc catgaagcac gcccacgcgc
     7801 gccgcgccga tttcgatttt cgctggcccg aaccaccgct gaatgtggtg ctggtcagcc
     7861 cggaaattcc gcccaatacc ggcaacatcg cgcggctctg cgcggccact ggcacgcggc
     7921 tgcatctggt ggagccgctg ggtttccaga tcaccaacgc ccaactgcgc cgcgcggggc
     7981 tggactattg ggatgacgtg aatgtgcgag tccacgcctc gtggccggcc ttcctcgctg
     8041 ccgcgcaacc cgaccgcctg tttctgtata gcaccggcag cgcgcgtcgc tatgaccgcg
     8101 tggcctaccg ccccggcgac acgctcgtct tcggctgcga aaccaagggc ctgccggatg
     8161 agctattggc cgcgcacgcg gaatcagtat gcggcatccc catccagacc cgccatgtcc
     8221 gttcgctcaa tctgtccagc gcggtggcgg tggtggtgta tgaagcgctg cggcagctca
     8281 acgcaccgta agcgggctca cgcccagtcg gcggtgtcca gcagcagtgt cacggggccg
     8341 tcgttgacca gttccacctg catcatggtg cggaacacgc ctgtctccac gccgaacccc
     8401 agcccgcgca agtgggccac aaacaattcg tagagcgcct cggcttgttc cggcggcgcg
     8461 gcttcgatgt agctgggccg attgccgcgc tcggtattcc ccagcaaggt gaactggctc
     8521 accactatga acttgccgtc cggcaccgcc gcgatggata gattcatctt gccgtcggca
     8581 tcgtcgaaga tgcgcagctt ggccaccttg tcggccagct tggcggcttc cgcggcggta
     8641 tccgtgcggc ccacgcccag cagcaccacc gcgccggggc cgatgcgccc cgtcacgcgc
     8701 ccctcgctgc gcacttccgc gcgcgccacc cgttgcacca ccgcccgcat cgccggttat
     8761 tgcaccgggc cgaccagcag ctcaatggtt tcggactgga tcagcgcgcc cgacaggtgc
     8821 cgcacaatcc gcgcctcgtt gcggaattgg cggaacaaat tgatctgcgt attcgcgtag
     8881 gcgcggccga gaatgcccgg caatggcaag tggcccagct tggcgccggt caccaccagt
     8941 tgatcgtttt tgacctgcac ataaaacagg ctggtgaacg tcagcggccc gcggctgatg
     9001 gccagataaa tttcggcgcg ccccggaaag aagcgcgcac ccgcgtcttg cagcgcggcc
     9061 gccagcacgc cgtgggccgc cggctgcgcc gccaccacgc cgcgcagata ggcgttgatt
     9121 tctttttctt cgatgacctg cgaggccggc acctcgcggt tcagcgcgtc aatcagcagt
     9181 tcgcccttca ggcggtagcg cttggcgtcc agttcctcgc cgacggtgcg caccatgcgc
     9241 gccggccaaa tggtcagcag aacaaccacc agcatgccca gcgccaccgc cagccggacc
     9301 agcgtgcgcg tttgttgggc cagcgtgggc cggtcggccg tcatcatttt ctgctcggcc
     9361 ttggcaaagt tcagtttggt gccgcacttc ttgcaataca ccgacggcag gcggttcatt
     9421 tcaccgcact ggtcgcaacg caacagcagg tcttccgagg ccatgatcag gctcctttct
     9481 tttccgcgcc ggctgccggc ttggactccg ccggcttgga ttcggccggc ttgtcaccgg
     9541 acgtggcgct agtgctggga gatttcgcgt tcttgtaatc cgtctgataa aat
//
LOCUS       DAOPNP010000094         9498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8143_length_9498_cov_8.945674, whole genome shotgun sequence.
ACCESSION   DAOPNP010000094 DAOPNP010000000
VERSION     DAOPNP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_9498_cov_8.945674"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1368
                     /locus_tag="PKI64_08475"
     CDS             64..1368
                     /locus_tag="PKI64_08475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOE01078.1"
                     /translation="MKISVVGTGYVGLVTGACFSDAGHEVMCIDNDPRKIEMLHRGEM
                     PIYEPGLDVIVERNVKAGRLKFSTSIAEGTAFAEVIFIAVGTPPGVGGEANMTYVEQV
                     GREVAEHMTSYRLLVEKSTVPAKTSEHLKRTVLKYLREDTDFDVASNPEFLREGTAIE
                     DATHPDRIVVGVESQRAGDLLEEIYRPILEKGGGEFLRMSVTSAELTKHASNSFLAMK
                     ISYINAVARICELAGADVQQVARGMGLDKRIGPRFLHAGVGYGGSCFPKDVDAFVRLS
                     DHLGYDFKLLKEVQMINKTQRDYVMKKIKQELWVVQDKRIAIFGLAFKPETDDVREAP
                     SLYFVPKLQEMKAKLRAWDPVAEGKFKEIHPDLECVQDLYECAKDADLVLILTEWDAV
                     KNIDLKKLKEVMACPVIVDGRNIFKPAEVRALGFTYHSVGRG"
     gene            1566..2051
                     /locus_tag="PKI64_08480"
     CDS             1566..2051
                     /locus_tag="PKI64_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOE01079.1"
                     /translation="MTTRIHYVQAAPEGIAPLIKLESYLKTSGLEPTLLELVKLRASQ
                     LNGCAYCIDMHTKDARSHGESEQRLYGVSAWHEAPFYTARERAALAWTEAVTLVSQSQ
                     VPDEVYAQVRAHFSEKETVDLTLAIAAINSWNRLAISFRAEAGSYQPQHPVTQRGVPP
                     A"
     gene            2048..2971
                     /locus_tag="PKI64_08485"
     CDS             2048..2971
                     /locus_tag="PKI64_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOE01080.1"
                     /translation="MTERVRHLHPAAPYRLAWAVWALRRRPENQVDRWDGHAYRRVFV
                     TPSGAPVVVKVTQAGPATKPELVVTMTGAKVPASFQQEVLATLERMLGVQTNLAGWQR
                     LVARDPRLAALAQPWRGFRPPRYASVFEALVNAVACQQLTLTMGVRLVNQLAAAYGVA
                     SSNAEGPCYSFPTPAALARANHDDLRRLKFSYRKAAYLTTLAQAVESGALNVVALETM
                     DDREVVERLCRLPGVGRWSAEYVLLRGLGRTHVFPADDVGARNHLQRWLALPEPLDYA
                     GVQHVLRDWQPYGGLIYLHLLLKGIAERGWM"
     gene            complement(3014..4855)
                     /locus_tag="PKI64_08490"
     CDS             complement(3014..4855)
                     /locus_tag="PKI64_08490"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOE01081.1"
                     /translation="MTSYDIAMLLFQVLGGLSLFIFGMNAMTSSLRTAAGSSLRTILA
                     RATRSRVHGSVFGTIFGFLAHSGAAVAALASFINIGVMTLEQAIAPVFGANIGTSLSM
                     LLVSFKIADYCWAVIGIGFLTEALVPSQRWRKLGGALIGFGLLFLGMDTIATGIAPYK
                     EALEPWLGLFRGDIWTYRLASVAVAALLTAIMTSSGAMIGLCFALVKAGVFTRFDQVA
                     VVILGAHIGTCIVPIVAAIPMRIGAWRVAIAHLWFNVLHVILFLVVWPLSIWCGEATA
                     PGNLLRQAANFNTYTMLVGTLLFLPFIKQFTALVRLSTPSNEPIPTPSFLDDKLLRKP
                     EQALAAVVRELRRMAEVCVENMLLNGELILAPTRQVYRKLLANEEILNDVRKAMDDYL
                     GQLTHRYLSRRQTLFLQHLDRCMKDIERIGDHLTHIGDTSRERARIPDALLPEDLFNT
                     WFNLFLAAKHVITLLAKSFDPDSNTFQNTALEVLRARDAYMEQSMDAKARFTDATRDK
                     RITPIGAYYVSRYIEDLDRLVRRAKSIALAERQPDFFVKASKLDRKAKDALAYTPPTP
                     VDARKYLALLQSDHHLDDADLPDEAASAIPLESPHQAPPDEHPATPE"
     gene            5294..6538
                     /locus_tag="PKI64_08495"
     CDS             5294..6538
                     /locus_tag="PKI64_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HOE01082.1"
                     /translation="MKQMKYLAGTVSVAALLAAQTVFGAGFGIYEGSARGNAMGTEVT
                     ADPASPSVLYNNPAAITELEGTQVEVGATFIRPAQTVTTMTPAGEVKNYADSRWWTPP
                     NAYVTHQFNDKIWTGLGVFSRYGLGIEFEDNWLGRYNCQEATIQSIDINPSVAFKVLD
                     NLSLAAGLRAEWFEFELFRALPTGAPFVDPDLQLRIKGDSWGLGYNLGAFFKATEWLS
                     LGVAFESEVDQDVEGDYSVKHPAGNIINNGDGKGDVTTPAIIRLGASIKATDKLTLNA
                     GVVYTMWSSYDQLAIEFDPALLGMVPASVTEKDWDDVFRYQFGVEYALDETWALRAGY
                     IFDETPDPDAHVDYIVPANDRHLFSVGVGYKKDNFFCDLGYTYLMIRDRDVAERQADG
                     VVEGKFKDGDAHMVGVSVGYKL"
     gene            complement(6605..7441)
                     /locus_tag="PKI64_08500"
     CDS             complement(6605..7441)
                     /locus_tag="PKI64_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOE01083.1"
                     /translation="MTQPPTNAERPPRESLRERLRQKAVVAYYMVEDFIPRPPNFAAW
                     LRLVRAPNLLTVPGDILAGFLLAPAATTGDWGQLLLAIPAGLLLYAAGLILNDIFDYA
                     EDLRERPARPLPSGAVSREAAVAVALICLWVAAFLAAFFDALPVALLLILMIILYDAG
                     MKRHPVVGPLLMGGCRAANLLMGATVAADRVWAGPIPLAGALLLGIYIAGVTALARNE
                     THAGTSFPPERIGRLLRMLIPLQALLGVAAIHRFPFNLLALVLLPLLPLHRRLARRFP
                     PS"
     gene            complement(7416..8264)
                     /locus_tag="PKI64_08505"
     CDS             complement(7416..8264)
                     /locus_tag="PKI64_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE01084.1"
                     /translation="MIIIEPHIHMLSRTTDDYTAMYQAGIRCVVEPSFWLGANRRHAG
                     TFFDYFQLSLEFEHTRAQRYGVDHWSCISVNPKEAEDLPLARETLAGIGPYLDHPRCV
                     AVGEIGFNRITHNEEVVFLEQLKLAKKHNLLVLIHTPHDTPEVSKKAGTARILDILRE
                     FNYDTGRIIVDHNTEDTMPLTRKTDYWAGLTVYPYSKLNPRRVVDILQRWGLERVLVN
                     SSADWGVSDPCSLPKTATFMAQQGFSPAQVTRLMHDNPLAFYSQTPKFKPRLDLPYID
                     PATYQR"
     gene            complement(8304..8675)
                     /locus_tag="PKI64_08510"
     CDS             complement(8304..8675)
                     /locus_tag="PKI64_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOE01085.1"
                     /translation="MKPIHTDNAPAAIGPYSQAIEHQGLLFISGQLGLDRVTGELVAC
                     TEAQTRLALQNLQAILAAAGLGPQNVVKATIYVANMADFALVNRLYAEVFSPPYPARE
                     VVQAAALPKGGLVEISAIAAQ"
     gene            8852..9400
                     /locus_tag="PKI64_08515"
     CDS             8852..9400
                     /locus_tag="PKI64_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOE01086.1"
                     /translation="MRKWVLVLMMVGLCATMASAAGGLGIFGSYWDTKDLGPGFGGGV
                     KFSGDLTEFLAIELRASCVTKFDDWDGDDELFIIPLEAGLIFNLPLGEDVPLTLYGGG
                     GGGYAIIPEADDVDLDDEFCFYGVAGAAFALNESIAIFGEAQYRFLEVDGVKVDGRRI
                     DTGGEKAKLSGLGVNVGLRFSF"
BASE COUNT         1938 a         2641 c         3122 g         1797 t
ORIGIN      
        1 ccgcggaatc ccataaggtg cgggcttcgg ttcacagatt cggacgttag gagagaaggc
       61 attatgaaaa tatcggttgt cggaacaggt tatgtcgggt tggtgacggg cgcgtgtttc
      121 tcggatgccg gccacgaagt catgtgcatt gacaacgatc cgcgcaaaat cgagatgctg
      181 caccggggcg aaatgccgat ctatgagccg gggctggatg tcatcgtgga gcgcaacgtc
      241 aaggccgggc ggctgaaatt cagcacttcg atcgccgaag gcaccgcgtt cgccgaagtg
      301 attttcattg ccgtgggcac cccgccgggc gtgggcggtg aagccaacat gacctatgtg
      361 gagcaggtgg gccgcgaagt ggccgagcac atgaccagct accgcctgct ggtggaaaaa
      421 agcaccgtgc ccgccaaaac cagcgagcac ctcaagcgca ccgtgttgaa atacctgcgc
      481 gaagacaccg actttgacgt ggcctccaac ccggaattcc tgcgcgaagg cacggcgatt
      541 gaagacgcca cgcatcccga ccgcatcgtg gtgggcgtgg aaagtcagcg cgccggcgac
      601 ctgctggaag aaatctaccg tcccattctg gaaaagggcg gtggcgaatt tttgcggatg
      661 agcgtgacca gcgccgagct gaccaagcat gcttccaact cgttcctggc catgaagatt
      721 tcctatatca acgcggtggc gcggatttgc gagctggcgg gcgcggatgt ccagcaggtg
      781 gcccgcggca tggggctcga caagcgtatc gggccgcgct tcctgcacgc aggggtgggc
      841 tatggcggct cgtgcttccc gaaagacgtg gatgccttcg tgcgcttgag cgaccacctt
      901 ggctacgact tcaagctgct caaggaagtg cagatgatca acaagaccca gcgcgactac
      961 gtgatgaaaa agatcaagca ggaactctgg gttgtgcagg acaagcgcat tgccattttc
     1021 ggtttggcgt tcaagccgga gacggacgac gtgcgtgaag cgcccagcct ctacttcgtg
     1081 cccaaactcc aggaaatgaa ggcgaagttg cgcgcgtggg accccgtggc cgaaggcaag
     1141 ttcaaggaaa tccacccgga tttggagtgc gtacaggatt tgtacgaatg cgccaaggat
     1201 gccgatttgg tcctgattct caccgagtgg gatgcggtca agaatattga cctgaaaaag
     1261 ttgaaagaag tgatggcgtg ccccgtgatt gtggacggcc gcaacatctt caagccggcg
     1321 gaagtgcgcg cgctgggttt cacctatcac agcgtgggcc gcggctaagc cacgtcgcca
     1381 gcatgttcca agaaacgccc ggccggcttt cgcgccgggc gttttgttgc tcggtggcag
     1441 ccgcgcccgg tggaatgtgc gcaacaaaac gcaaggcagc atttgcggcc cgccgcaacc
     1501 cggcataggt tgaacaaagg ggccgcgcgc gtggagcgcc ggccgccaac acaaggcagg
     1561 tcaacatgac aacacgcatt cattatgtgc aggcagcgcc ggagggcatc gcgccactca
     1621 tcaagctgga aagttatctc aagacatcgg gcctggagcc aacattgctg gaattagtga
     1681 agttgcgggc ctcgcaactg aacggctgcg cctactgcat tgacatgcac accaaggacg
     1741 cgcgcagcca tggcgaaagc gaacaacggc tctatggcgt gagcgcgtgg cacgaagcgc
     1801 cattttacac cgcgcgagaa cgcgccgcgc tcgcttggac ggaagcggtc accttggtca
     1861 gccaaagcca agtgcccgac gaagtgtatg cccaggtgcg cgcgcatttt tctgaaaaag
     1921 agacggtgga tttgacgctc gccattgccg ccatcaattc gtggaaccgc ctggccatca
     1981 gtttccgggc ggaagcggga tcgtatcagc cgcagcatcc ggtcacgcaa cgcggcgtcc
     2041 cgccggcatg accgagcggg tgcgccatct gcatccggcc gcgccatacc ggttggcgtg
     2101 ggcggtgtgg gcactgcggc gccgcccgga aaatcaggtg gaccgctggg atggccatgc
     2161 ctaccggcgc gtgtttgtga caccaagcgg cgcgccggtg gtggtgaagg tgacgcaggc
     2221 cgggccggcg actaagccgg agttggtcgt gaccatgacc ggcgccaagg tgcccgcaag
     2281 ctttcagcaa gaagttctgg ccacgctgga acggatgctg ggcgtgcaaa ccaatctggc
     2341 tggttggcag cggctggttg cgcgtgaccc gcgcttggcg gccttggccc agccatggcg
     2401 cggctttcgc ccgccgcgat atgcttccgt ttttgaagcg ctggtgaatg ccgtggcgtg
     2461 ccagcaatta acgctcacca tgggcgtgcg gctggtcaat cagttggctg cggcttacgg
     2521 cgtggccagc tccaacgcgg aaggaccgtg ctattctttc cccacgccgg cggcgttggc
     2581 acgcgccaac catgacgatt tgcggcggct caagttcagc tatcgcaagg cggcttacct
     2641 caccacgctg gcccaggcgg tggagtcggg cgcgctgaat gtggtcgcgc tggaaaccat
     2701 ggatgatcgg gaagttgtcg agcggctttg ccgtttgccg ggcgtgggcc ggtggagcgc
     2761 ggaatatgtg ctgttgcgcg gcttgggccg aacgcatgtg tttcctgcgg atgacgtcgg
     2821 cgcgcgcaac catctgcaac gctggttggc gctgcccgaa ccgctggatt atgccggcgt
     2881 gcaacatgtg ctacgcgatt ggcaacccta cggcggcctg atctatttgc acctgctgct
     2941 gaaggggatc gcggagcggg gctggatgtg acaatgcgca gggggggagg gcagcccgcg
     3001 ggcggtggcc gcatcactcc ggcgtggcgg gatgctcgtc cggcggcgct tggtgcggcg
     3061 attccagcgg aatggcggaa gcggcttcgt cgggcaggtc ggcatcgtcc aagtggtggt
     3121 cgctttgcag gagggcgaga tatttgcggg catccaccgg cgtgggtggc gtgtaggcca
     3181 gggcatcctt ggctttgcga tccagcttgg acgccttgac gaagaaatcc ggctggcgtt
     3241 ctgccagcgc gatggatttg gcccggcgga ccaagcggtc cagatcctcg atgtagcgcg
     3301 aaacataata tgcgccgatg ggggtgatgc gtttgtcgcg ggtggcatcg gtgaagcgcg
     3361 ccttggcatc catggattgc tccatatagg cgtcgcgcgc gcgtagaact tccagcgcgg
     3421 tgttttggaa ggtgttggaa tcaggatcga aggatttggc cagcagcgta atgacgtgtt
     3481 tggcggcgag gaacagattg aaccaggtgt tgaacaaatc ttccggcagg agggcatcgg
     3541 gaatgcgcgc ccgctcgcgc gaggtgtcgc caatgtgcgt gagatggtcg ccaatgcgtt
     3601 cgatgtcttt catgcagcgg tccagatgct gcaaaaaaag cgtttggcgg cgggagaggt
     3661 agcggtgggt gagttgcccg agatagtcgt ccatggcctt gcggacatcg ttgaggattt
     3721 cttcgttggc cagcagtttg cgatagacct gccgggtggg cgcgaggatc agttcaccgt
     3781 taagcagcat gttttccacg cagacctccg ccatgcggcg caattcgcgc acgactgccg
     3841 ccagcgcttg ttccggtttg cgcaggagtt tgtcgtccag gaatgacggc gtgggaatgg
     3901 gctcattgga gggagtgcta agccggacca gagcggtgaa ctgcttgatg aagggcagga
     3961 acagcagggt gcccaccagc atggtgtagg tgttgaaatt ggccgcctgc cgcaacagat
     4021 tgccgggcgc ggtggcttcg ccgcaccaaa tggatagcgg ccaaacgacc aggaacagaa
     4081 tcacgtgcag cacgttgaac cacaggtgcg caatcgccac ccgccacgcg ccgatgcgca
     4141 tggggatggc cgccacaatc gggacgatgc acgtaccgat gtgcgcgccc aaaatcacca
     4201 cggccacttg gtcaaagcgg gtgaatacgc cggccttcac cagcgcgaag cacaggccga
     4261 tcatcgcgcc ggatgacgtc atgatggccg tcagcagcgc cgccaccgcc acactggcga
     4321 gacggtacgt ccagatgtcg ccccggaaaa gccccagcca aggctccaag gcttctttat
     4381 acggggcaat accggtggcg atggtgtcca tgcccagaaa aagcaggcca aaaccgatca
     4441 gcgcgccgcc cagtttgcgc cagcgttgcg agggcaccag cgcttccgtc agaaagccga
     4501 tgccgattac cgcccagcaa taatcggcga tcttgaatga aaccaacagc atggaaagcg
     4561 acgtaccgat gttggcgcca aacaccgggg caatggcctg ttcgagcgtc atgacaccga
     4621 tgttaatgaa tgaggccagc gcggccactg ccgcccccga gtgcgcgaga aagccgaaaa
     4681 tggtgccaaa caccgagccg tgaactcggc tgcgggtcgc tcgagccaga atggtgcgca
     4741 gggagctgcc cgcggctgtg cgcaagctgc tggtcatggc attcatgcca aagataaaca
     4801 ggctcaaccc gcccagcact tgaaacagca acatggcaat atcgtacgac gtcatctcaa
     4861 cactccacac cgccggcgtg gccgcccgcg gagaaccgcc actatgcgcc cgcgttgttg
     4921 caaaacgcaa gaatggaatc gaaaaaaatg tgattgcgtc ggcgtccttc tacaccggca
     4981 acagaaaccc gcccgggcgc ggtggtatgt cagaggtcag gtgaaaaccg tggctgtatc
     5041 ctgatcgctg cctcatccta ctgcgttcac tatagttgcc agccacccct cacgggatgc
     5101 tcaacgaagg gcttgggcgg tcatcaaaaa aattggaaaa accggaaaaa gggcttgcgc
     5161 gggggtgggg ccttgggttc caatgggcaa atcttccatt tccaccgtgg agatggaggg
     5221 gttgaaccgg cgagggttgc ggcggtgaaa cgctccttcc ttgccgatgt aagataaata
     5281 aggaaggaac ctgatgaaac agatgaagta cctcgccggg acggtcagcg ttgcggcgtt
     5341 attggccgcg caaaccgtgt ttggcgcggg gtttggtatc tatgagggta gcgcgcgcgg
     5401 caacgcgatg ggcacggaag tcaccgcgga tccggcgtcg ccttcggtgt tgtacaacaa
     5461 cccggcggcc atcacggagc tggaaggcac ccaggtcgaa gtcggcgcga catttattcg
     5521 cccggcccag accgtgacga ccatgacccc cgccggcgag gtgaaaaact acgcggacag
     5581 ccgctggtgg actcccccga atgcctacgt gacgcaccag ttcaatgata agatttggac
     5641 gggcttgggc gtgttcagcc gctatggctt gggcatcgag ttcgaagaca actggcttgg
     5701 tcgctacaac tgccaggaag ccacgatcca aagcattgat atcaaccctt ccgtggcctt
     5761 caaggttctg gataacctct cgctggcggc cggcctgcgg gcggaatggt ttgagttcga
     5821 actgttccgc gcattaccga cgggcgcccc gtttgtggat cccgacctcc aactgcgcat
     5881 caagggcgat agctggggtc tgggttacaa cctcggcgcg ttcttcaagg ccaccgaatg
     5941 gctatcactc ggcgtggcat tcgaaagcga agtggatcag gatgtcgaag gcgattactc
     6001 ggtcaagcac ccggctggca acatcatcaa caacggtgat ggcaagggcg atgtgacgac
     6061 cccggcgatc atccgcttgg gcgcctcgat caaggccacc gacaagctga cgctgaatgc
     6121 cggcgtggtg tacaccatgt ggagcagcta cgaccagttg gccatcgagt tcgatcctgc
     6181 gctgttgggc atggtcccgg ccagcgtgac ggaaaaggac tgggatgatg tgttccgcta
     6241 tcagttcggc gtggaatatg cgttggatga gacctgggcc ctgcgcgcgg gttacatctt
     6301 tgatgaaacg ccggatcccg atgcgcacgt ggactacatt gtcccggcca acgaccgcca
     6361 cctgttctcg gtgggcgtgg gctacaagaa ggacaatttc ttctgcgacc ttggctacac
     6421 ctacctgatg atccgggatc gcgatgtcgc ggagcggcag gcggatggcg tggtggaagg
     6481 caagttcaag gatggcgatg cccacatggt gggtgtctcg gtcggctaca agctgtaatc
     6541 cttaccgcct agtgcaacaa aagccccgcg gccacggctg cggggctttt tcatggctca
     6601 acgctcaact cggcgggaag cggcgggcta gccggcggtg caagggcagc agtggcagga
     6661 gaaccaacgc cagcaaattg aaggggaagc ggtggatggc ggccacgccc aacagcgctt
     6721 gcagcgggat cagcatgcgc agtaggcggc cgatgcgctc cggcgggaat gaggtgccgg
     6781 cgtgtgtttc gttgcgggcc aaggcggtga ctcccgcaat gtagatgcca agaagcaacg
     6841 cccccgccag cggtattgga ccggcccata cgcgatccgc ggcgaccgtc gcgcccatga
     6901 gcaagttggc ggcacggcaa ccgcccatca gcaacggtcc gaccaccgga tgccgcttca
     6961 ttcccgcgtc gtacaaaata atcatcaaaa tcagcagtag cgccacgggc agcgcatcga
     7021 aaaacgcggc cagaaatgcg gccacccaca ggcagatgag cgcgacggcc acggccgctt
     7081 cgcgcgacac ggcgccggaa gggagcgggc gtgccggtcg ttcgcgcagg tcttcggcgt
     7141 aatcgaagat atcgttcagg atcaggcctg cggcgtaaag cagcaagccg gcggggatgg
     7201 ccagcagcag ttggccccag tcgccggtgg tggcggcggg ggccagtaga aatccggcca
     7261 gaatatcgcc gggtactgtc aacagattgg gcgcgcggac caagcgcaac caggcggcaa
     7321 aattcggggg gcgggggatg aaatcctcca ccatgtagta ggccacaacg gctttttgcc
     7381 gcagccgttc ccgcaggctt tcgcgtggcg ggcgctcagc gttggtaggt ggctgggtca
     7441 atatagggca ggtccaggcg gggtttgaac ttgggggttt gggagtaaaa cgccagcgga
     7501 ttgtcgtgca tgaggcgggt cacttgcgcg ggagaaaagc cctgttgcgc catgaatgtg
     7561 gccgtcttgg gcagggagca ggggtcggat actccccaat ccgccgagga gttgaccagt
     7621 acgcgctcca agccccagcg ctggagaatg tccaccacgc gccgcgggtt gagtttggaa
     7681 tacggataga ccgtcagccc cgcccagtag tccgtcttgc gggtcagcgg catggtgtct
     7741 tcggtgttgt ggtccacaat gatgcgaccg gtgtcgtagt tgaactcgcg cagaatatcc
     7801 agaatgcgcg cagtgccggc ttttttgctc acctcgggcg tgtcgtgcgg agtatggatc
     7861 aacacgagca gattgtgttt tttcgcgagt ttgagctgtt cgagaaacac aacctcttcg
     7921 ttgtgggtga tgcggttaaa gccgatctcg cccacggcca cgcagcgcgg gtgatccaga
     7981 taggggccaa ttcccgccag cgtttcgcgc gccagcggca ggtcctcggc ctctttgggg
     8041 ttcacagaga tacacgacca atggtcaacg ccgtagcgct gggcacgggt atgctcgaac
     8101 tccagggaaa gttgaaaata atcgaaaaac gtgccggcgt ggcgacggtt ggcgcccagc
     8161 caaaatgacg gctccaccac gcagcggatg cccgcctgat acatggcggt gtagtcatcc
     8221 gtggtgcgtg ataacatgtg aatgtgcggt tcaatgatga tcatggcttg ctcccgtcga
     8281 agatggattg gccgcgcaca ggtttattgc gcggcaatgg ctgaaatttc gaccaagccg
     8341 cctttaggca atgcggccgc ctgaacgact tcccgcgcgg gatagggcgg gctgaacacc
     8401 tcggcgtata gccgattgac cagtgcgaag tcggccatgt tagccacata aatggtggct
     8461 ttaaccacgt tttgggggcc aagcccggct gccgccaaaa tggcttgcaa gttttgcagc
     8521 gccaaacggg tttgggcttc cgtgcaggcc accaactcgc cggtgacccg gtcaagcccc
     8581 aattgcccgg aaatgaacag cagcccttgg tgctcgatgg cttgtgaata gggcccgatg
     8641 gcggcagggg cgttgtcggt atggattggt ttcatggttg cactcctcaa aacagtttca
     8701 tcctagcccg tggatggcag caggacaagc ctttgcgcaa ttttgtcgtt gtttgagctt
     8761 gcctgcacct cgcttgcgag gctacgctaa cactcgacag ggcggcgggt gctgtccgtc
     8821 gtagagggtc acaacaagga gaaaatcggt catgagaaaa tgggttcttg tgttgatgat
     8881 ggtggggctg tgcgccacga tggcgtctgc cgcaggcggg ctggggatct tcgggtccta
     8941 ttgggatacc aaggacttgg gcccgggatt cggcggcggc gtcaagttct ctggagatct
     9001 tacggaattc ctggccattg agctgcgcgc ctcgtgcgtg accaagttcg acgactggga
     9061 tggcgacgac gagctgttca tcatcccgct cgaagctggc ttgatcttca atctcccctt
     9121 gggcgaagac gtaccgctga cgctgtacgg cggtggcggc ggcggttatg ccattattcc
     9181 ggaagctgac gacgttgatc tcgacgacga attctgtttc tacggagtgg ccggtgccgc
     9241 ttttgcgctc aacgaatcca ttgcgatatt tggcgaagcc cagtaccgct ttttggaggt
     9301 tgatggcgta aaggttgacg ggaggagaat agatactggc ggcgagaagg ccaagttgag
     9361 cggcctcggt gtaaacgtcg gcctgcggtt ctcattctaa ccgtgcgcta gtccaagtat
     9421 ctgatcagtc aatccgcgtt gccgtcgtat tctgacggca gcgcggattt tgtttgcgca
     9481 aataaaatca gtcacctc
//
LOCUS       DAOPNP010000095         9465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8194_length_9465_cov_7.219129, whole genome shotgun sequence.
ACCESSION   DAOPNP010000095 DAOPNP010000000
VERSION     DAOPNP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8194_length_9465_cov_7.219129"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PKI64_08520"
     CDS             <1..1156
                     /locus_tag="PKI64_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOE01087.1"
                     /translation="ANANIGSACSMPMLQALPMFALAGYILAGSRASERLLRITRAAF
                     GWMPGGLAMVSIVTCTFLTAFTGASGVTIVALGGLLLPALLKDGYREKAALGLVTSGG
                     NMGVLFAPSLPLILYAIIAQPISGEVTIDALFRAGIIPGLLALAVLCAYGVWAGLHAQ
                     IPRRPFAWGELGAALKEAKWELPLPFVVLGAIYGGFLVASEAAVITAAYVLVVEVLIY
                     REIPWRKLTGIIRDTVVLVGGVLLILGMGMAITNYLIDQEVPQLILDWVGTRIESPIV
                     FLIALNVFLLAVGCVMDIYTATVVIVPLLAPLAVRYGINPVHLAIIFLANLAIGYSTP
                     PVGMNLFIAAMRLNRPLMKLAAASLALMGLMLITLLLITYWPALSLVFVK"
     gene            complement(1669..1830)
                     /locus_tag="PKI64_08525"
     CDS             complement(1669..1830)
                     /locus_tag="PKI64_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOE01088.1"
                     /translation="MMKYLCTVCGYVYDPEVGDPDGGVAPGTKFEDIPDSWVCPVCGV
                     GKDSFIPQE"
     gene            complement(1850..2911)
                     /locus_tag="PKI64_08530"
     CDS             complement(1850..2911)
                     /locus_tag="PKI64_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOE01089.1"
                     /translation="MAGGKGERFWPLSTAQRPKQLLALAGGKPLIIQAVERLAGIVPP
                     GRVLIVTNQSLVAPIRAMLGEGSPVGILGEPVGRDTAAAIAAGAAWIKRRDPNAVFCV
                     LTADHIIGDLPIFRETLRQGLELCAQNDILLTIGIAPSEPSNAYGYIETGDLWQEAGG
                     ISFFKARRFVEKPDIETAKGYLATGRYAWNSGMFAWSVKSILQALHEFQPQLASVAER
                     WALCTDDHIFQEQLNADFPDLNKISIDYAVMEKAQNIVVCKGTFAWDDVGSWPALEAH
                     LPKDESGNAVLGDVETWVSKGNIVVGKEGHLTALVGVEGLVVVQADGVTLVCSKERSQ
                     EIKGLLGQLRGQQQREKLL"
     gene            complement(2985..5636)
                     /locus_tag="PKI64_08535"
     CDS             complement(2985..5636)
                     /locus_tag="PKI64_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01090.1"
                     /translation="MRQIRVGFAMASGLLAGLLVYAAVAATPDSYTKVEAASLVTSPQ
                     NSWARAILFADELVQAPAGRAQRLDRKNYLPMRLKVAGTVWVPEALAPKFQALVVGNT
                     YSFAGTVDQISRRYYIIVDACYVLQTAADMTQRWTDMLNPPTQTEAERQASMSEAAMQ
                     GLLIAAQNSLIKMAAANNVTVGQLIEAQVDGGQRIAEHIVAEALQGELRAQNKTAEEL
                     MIGSVLALLQKQAVLDESQKAAAENAARAEAAATETPSVETEPAVEVAAVEVPAVEPP
                     AIEIPVEEVAAEAAAPEVASTDVADVAPPGEPVEVPVEEPVVQAPEAVEPETVVAVEE
                     PQAVEPVSPEEEIAAVVETEPVLEAQSEIPAEETPVEAAIVEPTAEEPVKVAASEEKP
                     GRKAKKKKQKSREKAPVEVTPEEPAVEVVEAEPAGVEPAEMVEIEMQPGPTVEQEMAA
                     ADETPPRPLASNYNGEPIAPPPSSMLVVPLTGQPEVVPMVSMQPTKAELARQKKQAAL
                     AERERVRAERKAAEEAKAAAAKQAREEALAKQAAEREARLESKRVAEAEAKAAAEARK
                     EAQLLAAAEAQAAKEARQAELARQRAEEQRACEEAAALKAAEWKAQQEAKQAAAEAQR
                     LQKEEAIRIAQEQREAEKAMREAEALAKQAEAAAAQRAETEKRLAEMAARKAAAEEAI
                     RALEAEKEAALAQRQAEMEALAAAEREAMEAEQAAVRARQAEEIRIAQEVAEHERQMA
                     EEAAERLAAEETARQAAADELARLEQEARELEKQAAKTRQEIKRGTIETPATVAQVDV
                     EPEPAVVEPTQAPVTDVELRKQEAAARKAEWLAEREAKAKAKADAKAQAQERKAAAEE
                     AKREAAAELQKAITDGEVPEWMQPVQF"
     gene            complement(5699..7075)
                     /locus_tag="PKI64_08540"
     CDS             complement(5699..7075)
                     /locus_tag="PKI64_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOE01091.1"
                     /translation="MSDLFDQNPSPPKVDAALRRPLAVRMRPRVLEDIVGQDHILGPG
                     KLLRRAIEADRLTSIILYGPPGCGKTSLAEVIARATQRVFDRSSGVLSNVAGLRQALE
                     AARMRRVNSGRETILFIDEIHRFNKAQQDVLLPYVEEGAVTLIGATTHNPFFFINSPL
                     TSRSQIFQLEPLSVAAVTELLRRALKAPDGLGHLAIEADEAALAHLAAVCEGDGRRAL
                     NALEIAALTTAPAADGVIHITRAVTEESIQKKAVVYDHDEDEHYNTISAFIKSVRGSD
                     PNAALYWLAKMLYAGEDPRFIARRLVILASEDIGNADPRGLSLAVAAMQAVDFVGMPE
                     ARITLAQVTTYLATAPKSNAAYMGLEAAWADVKEGRTLPVPRHLRSTGSKRAAKEFGH
                     QGYQYAHDFPGHFVDQEYVPTDKVYYEPTTAGYEDMIRKRLEHWAGLRQAAREAAPPA
                     KPAGEGRG"
     gene            complement(7163..9274)
                     /locus_tag="PKI64_08545"
     CDS             complement(7163..9274)
                     /locus_tag="PKI64_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01092.1"
                     /translation="MRARCTGWLGWSIGWMLMALTAHAGVETFDNTGLAGSWVASGSF
                     TGQENVVWTYANARGTPTIYSNNPSITLRGGSAATKGWLLSGTITGGVEQLTATFKQV
                     LSAKADYDVWVNDRLITNYTSSGGSGSVETVTFPVHDPVNRLPFTNEFLLMISNRLAT
                     SGPLAIDDLEWKPFELFVRLDRVGTNLLYVGGEYDTHAEVFSLDQPLAGEWIIEPDFA
                     GTILGTETNLLTLIPVVADAGKTFTVTYRVTGTEDPDLTAETGCYLRVEEAPSARRIN
                     FEGVSFNYNTNAGEEVTLTNMPWLFFNVRTSGSTDKRIGATSARFRHSADSLPARMES
                     LDTFDGVGAVILHAAYYGSNRVVKFDVQSRGKDEESWTTHGSFNVQDCEDITNSIFVV
                     EIQRLGPTYIRLITTGNFNEIANVDDIFISEYDNLPPRVSWSGSTNVPVGWTSVVDFT
                     LHNAAGVVREWEWTLEPPNPNAEFAVTPDDQLQLVFAPTATNEWGNYTASATARSGGE
                     PLGSTSVVLRVVSPPSFTLAPVAANIVVPDVVDIWVTNVVLHAAGTNWTTAWLIEPPF
                     VNTSSVSNKSRFRIAAGTVLADVGTHLVNALLTDKGSGVTVTNSVLLTVTAGGGGDIT
                     NEMYFITAFDPQGQVTVSGKVGRVFQVFAASNLPAGPAQTNWVWQGTPITNTADGDVV
                     LPLPAPEGNMRFYGVIIRPAP"
     gene            9389..9465
                     /locus_tag="PKI64_08550"
     tRNA            9389..9465
                     /locus_tag="PKI64_08550"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9423..9425,aa:Val,seq:gac)
BASE COUNT         1711 a         2996 c         2628 g         2130 t
ORIGIN      
        1 ggccaacgca aacatcggca gcgcttgcag catgcccatg ctgcaagcgc tgccgatgtt
       61 tgcgttggcc ggctatattc tggcgggcag tcgggcctcg gagcggttgc tgcgcatcac
      121 ccgcgcggca ttcggttgga tgccgggggg gctcgccatg gtgagcattg tcacctgcac
      181 atttttgacc gcctttaccg gcgcgtcggg cgtgacgatt gtggccttgg gcggattgct
      241 cctgccggct ttgctcaagg atggctatcg tgagaaagcc gcgttggggc tggtcaccag
      301 cggcggcaac atgggtgtgc tctttgctcc cagcttgccc ttgattttgt acgccatcat
      361 tgcccagccg atttcgggtg aggtgaccat tgatgcgttg ttccgtgccg gcatcattcc
      421 cggcctactg gctttggccg tgctttgcgc ctacggcgta tgggccggtc tgcacgcgca
      481 gatcccacgt cgaccttttg cgtggggtga acttggcgcg gcacttaaag aagccaaatg
      541 ggaattgccg ttgcccttcg tggtgctggg cgccatctat ggcggttttc tggtcgccag
      601 cgaagccgcc gtgatcaccg ccgcatacgt gctggtggtc gaagtcctca tttatcggga
      661 aatcccatgg cgcaaattga ccggcatcat tcgcgacacc gtcgtgctgg tcggcggggt
      721 gctgctcatc ttgggcatgg gaatggccat caccaattac ttgattgacc aggaagtccc
      781 acagctcatc ttggattggg tcggcacgag aattgagtca ccgatagttt tcctcatcgc
      841 tctgaacgtc ttcttgttgg ccgtcggctg tgtaatggac atttataccg cgacggtggt
      901 catcgtgccg ctgctggctc cgctggcggt ccgctacggg atcaacccag tgcacctggc
      961 catcatcttt ttggcgaatc tggccatcgg ctacagcacg cccccggtgg gcatgaatct
     1021 gttcattgcc gccatgcggc tcaaccgccc gctgatgaag ctggccgcag cctcgctcgc
     1081 cctgatgggg ctgatgctga ttactttgct gctgatcact tattggccgg ccttgagctt
     1141 ggtctttgta aagtagccca gtcttacgac acgaggaggg gtgggtaggg cagggcaggg
     1201 gggggcatac cccctaccct gtgggtgtca cagaaataac cgatgaggcg cgcggataaa
     1261 accacgacgg gcaaagcctc acaaacatga cgcggaagtc ccatcatcct gacgccgacg
     1321 gggcgcacct tgtttgattc ttaagcagat aaagtccaac aggccttaag tgcttgttca
     1381 caagcccacc caagtcgctt ttacttcaat ccagtgggcg gaaatagcct cctcgagcgc
     1441 atgaattata ggcgctattc acaagttact cacaaagtta tcgtcggggc ataaatcacg
     1501 ctgaatcgcc aattgatcta tatgtagtat gcctgaccgg ccaatactta acataatata
     1561 tattatgcga cgtaatatga aaggtgtgga atgactggga ttttggcttg aactaacaaa
     1621 aaagcccggc cgaaccttgt agctcagccg ggctctgtat gaaggcggtt attcttgcgg
     1681 gataaatgaa tccttgccga cgccacacac cgggcaaacc cacgagtccg gaatgtcttc
     1741 gaacttggtg ccgggcgcta cgccaccgtc gggatcgccg acttcaggat catagacata
     1801 accgcaaacc gtacatagat atttcatgat gatctcctgt tgttttgttt tagagtagtt
     1861 tttcgcgctg ttgctggccg cgcaattggc ccaaaagtcc tttgatttcc tgcgagcgtt
     1921 ccttgctgca caccagtgtc acgccatctg cttgcaccac gactaaaccc tcgacgccca
     1981 ccaaggccgt caaatggcct tccttgccga ccacaatatt gcccttgctg acccaggttt
     2041 ctacatctcc aagcactgca tttccgctct cgtccttggg cagatgcgct tccaacgccg
     2101 gccaagatcc tacgtcatcc caagcaaatg tgcctttaca cacgacgatg ttctgcgcct
     2161 tctccatgac ggcgtaatca atggagattt tgttcagatc ggggaaatcg gcattgagct
     2221 gctcttggaa tatgtgatca tcggtgcata aagcccagcg ttcagctaca gaggccaact
     2281 gcggctgaaa ctcgtgcagc gcctgcagaa tgctttttac tgaccaggca aacatgccgg
     2341 aattccaagc ataacggccg gtggccaaat accctttggc ggtttcaata tcgggttttt
     2401 caacaaaccg gcgggccttg aaaaaagata ttccgcccgc ctcctgccaa aggtcgccgg
     2461 tttcaatgta gccatacgca ttgctgggct cagacggcgc aatgccgatg gtcagcaaaa
     2521 tgtcgttctg agcacacaac tccaaccctt gccgcagggt ttcccggaaa atcggcaaat
     2581 cgccaatgat gtgatcggcg gtcagtacgc agaatacggc gttaggatcg cggcgtttga
     2641 tccatgcggc gccggcggct attgcggcgg ctgtatcacg ccccaccggt tcgcccagaa
     2701 tgcccacggg actcccctcc cccagcatcg cgcgaatggg cgccacgagc gattggttgg
     2761 tgacaatcaa cacccgccct ggtggaacaa tgccggccaa gcgttcaaca gcctggatga
     2821 tcaagggctt gccgccagcc aatgccaaaa gctgcttggg gcgctgggcg gtgctcaagg
     2881 gccaaaagcg ctcgcctttg cccccggcca tgatgattgc atacgcgttg tgctcgcctg
     2941 ccatgattat tcccggtgcc ctaccttatc gttccattcg gcagttagaa ctgcaccggt
     3001 tgcatccatt ctggcacctc gccgtcggtg atggcctttt gtaattcggc agcggcctcc
     3061 cgcttggctt cttccgcagc agctttacgc tcctgtgctt gcgccttggc atcggccttg
     3121 gcctttgcct tggcttcccg ctcagccagc cattccgcct tgcgggcggc tgcctcttgc
     3181 ttgcgaagct caacatccgt caccggcgct tgtgtcggtt caaccacggc cggttccggt
     3241 tctacatcca cttgagccac agtggcgggc gtctcgatgg ttccgcgctt aatttcctgg
     3301 cgtgtcttgg cagcctgttt ttccagttcg cgggcttctt gttcaagacg cgccagttcg
     3361 tccgcagctg cttggcgtgc ggtttcttcc gcggccaagc gctcggcggc ttcctccgcc
     3421 atttggcgct catgttcggc cacttcttgg gcgatgcgga tttcctctgc ctgccgcgca
     3481 cggacagccg cttgctcggc ttccattgcc tcacgctcgg cggcagcaag agcttccatc
     3541 tcggcttgtc gctgggctag ggccgcttct ttttcggctt ccagggcgcg tatcgcttct
     3601 tcggcagcgg ccttgcgcgc agccatttct gccaagcgtt tttcggtttc cgcgcgttgg
     3661 gcggcggcag cctcggcctg cttggccaag gcttcagctt cgcgcatggc tttttcagcc
     3721 tcgcgttgtt cttgtgcgat gcggatggcc tcttcttttt gcagacgttg ggcttccgcc
     3781 gcagcctgct tggcttcttg ttgcgccttc cactcggcag ccttcagggc tgccgcttcc
     3841 tcgcaggcgc gctgttcttc cgcgcgctga cgggccaatt cagcttgccg tgcttccttg
     3901 gcggcctgtg cttccgccgc ggccaaaagt tgtgcttcct tgcgtgcttc cgcggccgcc
     3961 ttggcttcgg cctcagccac tcgcttggat tccagccgcg cctcgcgttc agcggcctgc
     4021 ttggccaagg cttcttcgcg cgcctgcttg gccgccgccg ccttggcttc ttccgccgcc
     4081 ttgcgctcag cgcggacgcg ttcgcgctcg gccaacgccg cttgcttttt ctgccgggcc
     4141 aactcggcct tggtcggctg catactgacc atcggcacga cttccggttg tccagtcaac
     4201 ggcaccacca gcatgctgct gggcggtggc gcaatcggtt cgccattgta attggaggcc
     4261 aaggggcgtg gcggggtctc atcggccgcc gccatttcct gttccacagt cggccccggc
     4321 tgcatctcga tttccaccat ttctgccggt tcaacgccgg ctggttccgc ctcaacaacc
     4381 tcgaccgccg gttcttccgg cgtcacttcg acaggcgctt tttcgcggga cttttgcttt
     4441 ttctttttcg ccttcctgcc cggcttttcc tcgctggccg cgaccttgac gggttcttcc
     4501 gccgtcggct ctacgatggc tgcttccacg ggcgtttcct cagccgggat ctcgctttgt
     4561 gcttcaagaa ctggctcggt ttccactaca gcagcaattt cctcttccgg agagacaggc
     4621 tcgacagctt gtggttcttc aaccgccacg acagtttccg gctccaccgc ctccggagcc
     4681 tgcaccacgg gctcttcaac cggtacttcc accggttccc ccggcggggc cacatctgcc
     4741 acatccgtgc tcgcgacttc cggagccgca gcctcggcgg cgacttcctc aaccggaatt
     4801 tcgattgctg gaggttcaac tgccggaact tcaactgccg cgacctcgac tgccggctcc
     4861 gtttccacgc tgggagtttc cgtcgccgcc gcttccgcgc gggcggcatt ttccgccgcc
     4921 gccttttgcg actcatccag caccgcttgc ttttgcagca acgccagcac ggagccaatc
     4981 attagttctt cagcagtttt attttgagcg cgcaattcac cttgcagggc ttcggccacg
     5041 atatgctcgg cgatgcgctg gccgccatcc acctgcgcct cgattaattg ccccacggtg
     5101 acattgttgg ccgcggccat tttgatcagg ctgttttgcg cggcaatcag cagaccctgc
     5161 atggcggctt cggacatgct ggcctgccgc tccgcttcgg tttgcgtcgg cggattgagc
     5221 atatccgtcc agcgctgggt catgtcggcg gcggtctgca gcacgtagca cgcgtccacg
     5281 atgatgtaat agcgccgcga aatctggtcc accgtgccgg caaatgaata cgtattgccc
     5341 acgaccagcg cttgaaactt cggcgccagc gcttccggca cccacacggt gccggcaact
     5401 ttcaggcgca tgggcaggta gtttttgcga tccagccgct gtgcgcggcc ggccggcgcc
     5461 tgcaccaatt cgtcggcaaa caagatggcg cgggcccatg agttttgtgg gctggtcacc
     5521 agcgatgcgg cttccacctt ggtgtaggaa tccggcgtgg ccgccactgc cgcatatacc
     5581 agcaaacctg ccagcagacc cgacgccatc gcgaagccga ctcgtatttg tctcatagcc
     5641 ttaacctttt cccaaaaata tggttatttg tataacgccc aacaagtcca aggcaagatt
     5701 atcctcgccc ctcgccggcg ggcttggccg gaggggcggc ctcccgggcg gcctgccgca
     5761 ggccggccca atgctccaac cgcttgcgga tcatgtcttc atagccggcg gtggtcggct
     5821 cgtagtacac cttgtccgtg ggcacatatt cctgatccac gaaatgaccg ggaaaatcgt
     5881 gggcatattg atagccctga tgcccgaact ctttggccgc gcgcttggag cccgtgctac
     5941 gcaaatgccg cggcaccggc aacgtgcggc cttccttcac atccgcccat gcggcctcca
     6001 agcccatgta ggccgcgttg ctcttgggcg cggtggccag atacgtcgtc acttgcgcca
     6061 gggtaatccg cgcttccggc atgcccacaa agtccaccgc ctgcattgcg gccaccgcca
     6121 ggctcagccc ccgagggtcg gcattgccga tatcctcgct ggccagaatc acaaggcggc
     6181 gggcgatgaa tcgcgggtct tctccggcat agagcatctt ggccagccaa tacagcgccg
     6241 cattggggtc cgacccgcgc acgcttttga tgaatgccga aatcgtgttg tagtgctcat
     6301 cttcgtcgtg gtcgtacacc acggcttttt tctggatgct ctcttccgtc accgcgcggg
     6361 taatatggat cacgccatca gccgccgggg cggtggtcaa ggccgctatt tccagcgcat
     6421 tcagcgcgcg gcggccatca ccctcgcaca ccgccgccaa gtgcgccagc gccgcctcat
     6481 ccgcctcgat tgccagatgg cccaaaccgt cgggggcctt cagcgcgcgc cgcagcagtt
     6541 ccgtcaccgc tgccacgctc aacggctcca attggaaaat ctggctgcgc gaagtcagcg
     6601 ggctgttgat gaaaaaaaac ggattgtggg tggtcgcccc gatcaaggtg acggcgccct
     6661 cctccacgta aggcaacagc acatcttgtt gcgccttgtt gaagcggtgg atttcatcaa
     6721 tgaacaaaat ggtttcgcgc ccggaattga cgcggcgcat ccgtgcggcc tccaacgcct
     6781 gccgcagccc ggccacattt gaaagcacgc cgctggaacg gtcaaaaacc cgctgcgtcg
     6841 cgcgcgcgat aacctcggcc agcgaggtct tgccgcagcc gggcgggcca tagaggatga
     6901 tgctggtcag ccggtcggcc tcgatggcgc gccgcagcaa cttgcccggc cccagaatgt
     6961 gatcctgccc cacgatgtct tccagcacgc gcgggcgcat ccgcacggcc agcggtcgcc
     7021 gcaatgcggc atccaccttg ggcgggctgg ggttttggtc aaacaaatcg ctcatgggta
     7081 aggtgcggaa accatgccag aaccgccgtg actacccaat catattttgc ccctcccccg
     7141 ccgccttggc ttgcacgccg gctcagggcg ccggccggat aataacccca taaaagcgca
     7201 tgttcccttc cggcgccggc aacggcagca ctacgtcgcc gtccgctgtg ttggtgatcg
     7261 gggttccctg ccatacccag ttggtttgcg ccgggcctgc cggcagatta gatgccgcaa
     7321 aaacttgaaa aacccgcccg accttgccgc ttacagtcac ctggccctgc ggatcaaagg
     7381 ccgtgatgaa gtacatttcg ttggtgatat cccccccacc ccctgccgtc accgtcaaca
     7441 gcacgctatt ggtgaccgtc acccccgacc ctttgtcggt gagcagggca ttgaccaggt
     7501 gcgttcccac gtccgccaaa acagttccgg ccgcaatgcg gaaacggctc ttgttgctga
     7561 ccgaggatgt attgacaaac ggcggctcga taagccaggc ggttgtccag ttggtgccgg
     7621 cagcatgcag caccacattg gtcacccaga tatccacgac atcgggcacc acgatgttgg
     7681 ccgccaccgg tgccaacgtg aatgaaggcg gcgacaccac gcgcagcacc acgctggtgc
     7741 tgcccagcgg ttccccgccg ctacgcgccg tggcgctggc tgtgtagttt ccccactcat
     7801 tggtggcggt gggggcaaac accaactgta attggtcgtc cggcgtcacg gcaaattccg
     7861 cgttggggtt gggcggttcc aacgtccatt cccattcccg gacaaccccc gcggcgttgt
     7921 gcagggtgaa atccaccacc gaagtccacc ccaccggcac attggtgctg ccgctccatg
     7981 acacccgcgg cggcaggttg tcgtattcgc tgatgaaaat gtcatctacg ttggcaattt
     8041 cattaaagtt gcccgtggtg atcagccgga tatatgtggg cccaagccgc tggatttcca
     8101 ctacaaaaat ggagttggtg atgtcctcgc aatcctgcac gttgaaagag ccgtgtgtgg
     8161 tccagctttc ttcatctttg ccacggcttt gcacatcaaa tttgacgaca cggttggacc
     8221 cataataggc cgcgtgtaaa atcaccgcac cgaccccatc aaaagtgtcg aggctttcca
     8281 tgcgcgcggg caggctatct gccgaatggc gaaaccgcgc cgacgtggcc ccaatccgtt
     8341 tatcggtgct accgctggtg cggacattga aaaacagcca tggcatgttg gtgagtgtga
     8401 cttcctcgcc ggcgttggtg ttgtaattga aactgacgcc ttcgaaattg attcgtcttg
     8461 ccgacggcgc ttcttccacg cggagataac aaccggtttc cgcggtcaaa tcaggatcct
     8521 cggtgcccgt cacccgataa gtgacagtga aagttttccc agcatccgcc acaacaggga
     8581 tcagagtcaa aagattggtt tccgtcccca gtatggtgcc ggcgaaatcc ggttcgatga
     8641 tccattcccc ggccaaaggc tgatccagcg aaaacacttc cgcgtgggta tcgtactcgc
     8701 cccccacata cagcaaatta gttcccaccc ggtccagccg cacgaataac tcgaatggtt
     8761 tccactccaa atcgtcaatc gccaatggtc cgctagtggc cagccgattg gaaatcatca
     8821 gcaagaattc gttggtaaac gggagccggt tgacgggatc atgaaccgga aaggtgacag
     8881 tttccactga gccagatccc cccgatgatg tgtaattggt gatcaaacga tcattcaccc
     8941 atacgtcata atcagctttt gccgaaagca cctgtttgaa agtggccgtg agctgctcta
     9001 cgccgccggt gatcgtccct gaaagcagcc agcccttggt ggccgccgaa ccgccacgca
     9061 acgtgatgga tggattattg gaatagattg ttggcgtacc acgggcattc gcgtacgtcc
     9121 agaccacgtt ttcctggccg gtaaacgacc cgctggccac ccacgagcct gccaaaccag
     9181 tattgtcgaa tgtttccacc ccggcgtgtg cggtgagcgc catcagcatc cagccgatgc
     9241 tccaacccaa ccagcctgta cagcgcgccc gcatctattc gttcattgaa acatactccc
     9301 ccgccacccg cgaggcaaaa accaattggc cgcgggcaaa aaaggattga acacccgcgc
     9361 ggttgtggca tagtccaccg cgacttccgg gcgattagct cagttggtta gagcgcttgc
     9421 ttgacatgca agaggtcaca gattcgagtt ctgtatcgcc cacca
//
LOCUS       DAOPNP010000096         9347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8325_length_9347_cov_8.865260, whole genome shotgun sequence.
ACCESSION   DAOPNP010000096 DAOPNP010000000
VERSION     DAOPNP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8325_length_9347_cov_8.865260"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKI64_08555"
     CDS             complement(<1..648)
                     /locus_tag="PKI64_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HOE01093.1"
                     /translation="MQLNLTPLEARILGALVEKELSTPEYYPLTLNSLVAACNQKNNR
                     DPVLQLATSEIEAALATLQYDKQLVGTFSGVGSRVLKYTHRLLETLAVDTPELAVLCE
                     LLVRGPQTPGELRGRTARLHTFASPAEVRRVLDALAARRPDPYVVELPRAPGSKENRF
                     AHALGDAPPATAPPPHKTAPSPMVAVVATADNPRIRELEQRIADLEAQIAALRKDG"
     gene            complement(699..1736)
                     /locus_tag="PKI64_08560"
     CDS             complement(699..1736)
                     /locus_tag="PKI64_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01094.1"
                     /translation="MTCRQILLATLLTLTTGTGALCAGVEWGGCLEPGRDQNLRLSFG
                     VVSEFEGMIAETRRKLYDVTGSTWKQDDAEIYSTSDFNLDGSYRAMGFSLDMAWAFVQ
                     LQLDSIFLNPTTSATARRDYYIAISDSIEYRGQSYDHLMIPAGAPFHAELFGNMTELN
                     FLLVPVGFNIGDFVRINPSWAFGLVLFGGRYEIDAGEPRGTKTYQNPPEEFVVGGQTS
                     GLTILGAPQWGPGVDLHLGQTEGVQVRLRVQYLFASYDGGTGWFTTADHREKNLDFSH
                     KNLRIRGQVEIPLNRVALTIGLQAQFIETDGTVSSSATDPADILARQERFDKEFSFRL
                     QSLLGTIGITF"
     gene            complement(1774..2529)
                     /locus_tag="PKI64_08565"
     CDS             complement(1774..2529)
                     /locus_tag="PKI64_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOE01095.1"
                     /translation="MKIKLFRLALLALVAGWLAACSSRTTTPPLVLGTDAKLPPFASR
                     SDTAEGGFAGFDIEIAKAIAAQAGRPLLIKELDFVDLLPALAADQVDFVVAAMSITPE
                     RRQAADFSEPYYNATPVVITLAGQPAPQTAEDLRDKRLAALPGSAGAALAGDLTSMEN
                     VLLMGALWDAVILLKNSGTDAIVLDEQPARALVQGDPEITLTQLDFPPDHYGVAVRTG
                     RADLLTTVNAALAAAKADGRYTRWLNQWLLLRD"
     gene            complement(2615..2944)
                     /locus_tag="PKI64_08570"
     CDS             complement(2615..2944)
                     /locus_tag="PKI64_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01096.1"
                     /translation="MTEFPLVIRTPDRECFSGPAESITVPALNGELGVLAGHLPLLAG
                     LKAGVVHVRTPGGGERWFAVDGGILGVERGSPVRLLAMQIADCASATAARAAAAGFQN
                     EAAPAAP"
     gene            complement(2941..4383)
                     /gene="atpD"
                     /locus_tag="PKI64_08575"
     CDS             complement(2941..4383)
                     /gene="atpD"
                     /locus_tag="PKI64_08575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOE01097.1"
                     /translation="MKPTATPPAKPRLGHITQVMGAVVDVEFPPGDLPPILHALTTTN
                     AAINDQPDNLVLEVAQHLGDSLVRAIAMHQTDGLVRGAEVRDTGREIEVPVGPGTLAR
                     VMNLLGEPVDGGGPITAAKRLPIHRPTPAFTDQSTQENILVTGIKVVDLLTPYRKGGK
                     IGLFGGAGVGKTVLIQELIHNIAKTYGGYSVFAGVGERTREGTDLYQEMKDSGVIDKT
                     VLVYGQMNEPPGARARVALSALTVAEYFRDEMQQDVLLFIDNIYRFTQAGAEVSAMLG
                     RIPSAVGYQPTLGTDMGELQERITSTKQGSITSIQAVYVPADDYTDPAPATTFVHLDA
                     TTELSRAMVELGIYPAVDPLTSSSSLLNPYVVGEEHYRVAQGVKEILQRYKDLQDIIA
                     ILGMDELSDEDRAVVARARRVQRLLSQPFFVAEQFVNIPGRFVPLEQTIRSFKEVLAG
                     QHDDVPEQAFYMVGSIDDVLAKAQTMKAQS"
     gene            complement(4412..5278)
                     /gene="atpG"
                     /locus_tag="PKI64_08580"
     CDS             complement(4412..5278)
                     /gene="atpG"
                     /locus_tag="PKI64_08580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOE01098.1"
                     /translation="MANYREYNAKLRQLQGMRRVTGAMKMLATTKLARAQAMLTQAQG
                     FGTEWRHLLRMAEHLVNRQNPLVAPRAKPENVFVLMLTADMGLCGGFNNNLIRLVGRW
                     LDENAARYRILRFSFCGRKGYQHFRRQVEVRAFYEQAVRHPDLTLARRLSREVFTPYV
                     EGKYDEIYLARNRYFSPLWQEPVVERLLPVDLSALPPAPAGREPDVLLEPHSPRLLTH
                     VLELHVCQRIFSALWENASGEHAARMTAMDSAAKNIDHLCQTYTLLRNRARQDAITTE
                     LTEVVAGAEALH"
     gene            complement(5297..6808)
                     /gene="atpA"
                     /locus_tag="PKI64_08585"
     CDS             complement(5297..6808)
                     /gene="atpA"
                     /locus_tag="PKI64_08585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOE01099.1"
                     /translation="MELKPAEISAILKRQIENFDRAAEVFESGTVLSVGDGIARIFGL
                     QQAMAGELVEFTNGVRGIVLNLEEQSVGVAIMGDAAGIREGDLVRRTRQIAAVPVGPA
                     LAGRVVDALGNPLDGGPPPDAAELRPLEAPAPGILDRQKVREPLQTGIKVIDALTPIG
                     RGQRELIIGDRKTGKTTLGIDTIINQADKNVHCFYVAIGQKRSTVTQVCEKLRQHGAM
                     AYTTIVAATAADPAPLQYLAPFAGCAMAEYWRDRGGHALIVYDDLTKQAQAYRQLSLL
                     LRRPPGREAYPGDIFYLHSRLLERAAKVSPARGGGSLTALPIVETQANDISAYIPTNV
                     ISITDGQIFLESSAFNAGQRPAINPGISVSRVGGDAQLKAMKKVAGTLRLTLAQYREM
                     AAFAQFAGDLDKATLQQLARGKRLMEAFKQGPQRPLPVSQQIIFLYACSTGACDDIPV
                     PQMAEYERQLGLALTGPHAALRERLEQTGDLTSDLKAELDAMLAAFNKTFVVA"
     gene            complement(6829..7371)
                     /gene="atpH"
                     /locus_tag="PKI64_08590"
     CDS             complement(6829..7371)
                     /gene="atpH"
                     /locus_tag="PKI64_08590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOE01100.1"
                     /translation="MAQPLAIIRRYAKALLDLATQNDSLARVKAEVETVQAWLQTTPD
                     FRTFVACRHLGEKPARLAALRELAQRAAFSPTMTEFLAQCEGAQLLEHLPRILAEFAR
                     RQQAQARVMPAEIVSARPLTGAQREVLLARLGAGRRLAPVFREAPELRGGFIAYIGDE
                     VHDYSVAGRLRRLRRRLTAG"
     gene            complement(7373..7909)
                     /gene="atpF"
                     /locus_tag="PKI64_08595"
     CDS             complement(7373..7909)
                     /gene="atpF"
                     /locus_tag="PKI64_08595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOE01101.1"
                     /translation="MNETQEMVAVPAPATEIGNVLQVSDQMVMLTWVAFLLACFALYK
                     LAWKPICQALDRREDKIRQSLQAAEDIRAQAELDKEAQRVMLANAKQEAAALVARAQE
                     AAAATAKAVEEKAAADAAQAVRAAQDAIRRAQEQAVGELRRTAAELALDVAGRVLDQK
                     LDAPADHALTDRLLQELE"
     gene            complement(7935..8159)
                     /gene="atpE"
                     /locus_tag="PKI64_08600"
     CDS             complement(7935..8159)
                     /gene="atpE"
                     /locus_tag="PKI64_08600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOE01102.1"
                     /translation="MTELAFKGLALASLGAGLVAIGAGVGIGFVWSAGLKGIARQPEL
                     AAKLQTSMFIGAALVEGIALFALVICFLQI"
     gene            complement(8208..9056)
                     /gene="atpB"
                     /locus_tag="PKI64_08605"
     CDS             complement(8208..9056)
                     /gene="atpB"
                     /locus_tag="PKI64_08605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOE01103.1"
                     /translation="MEPALEMVLPAVTNVVAGVVAAPDRKDIVHHYIQHHVLYHVADG
                     NPSAWNLPFIRVQALDVFRNDGVMLALALLLLVAGFAFLYRRQQGDVPRGWSNLLELF
                     VVFVRNQIVQPYFPERDARRFAPVFCAFFFLILTLNLLGLCPLFSAATSNINVTGGLA
                     LVAGAFMVGVTIWQKGVRGFLRTFVPTGAPWILLPVLVPVEVISFFSRVFALAIRLFA
                     NVLAGHILIFALLSMVVLFGWLASPIILLVVGIYFFEIFMSFLQAYIFSLLAAIFIGQ
                     MAYEGH"
     gene            complement(9057..9284)
                     /locus_tag="PKI64_08610"
     CDS             complement(9057..9284)
                     /locus_tag="PKI64_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01104.1"
                     /translation="MTGQSPFFAGGQQAIGLAWTIPVLLAVYLGLGYWIGGLLGARLA
                     GVVVGWLAGMGAVAYEIRKVLRRDAGNQDPR"
BASE COUNT         1633 a         2917 c         3157 g         1640 t
ORIGIN      
        1 tccgtctttg cggagggcgg cgatttgggc ttcgagatca gcgatgcgtt gttcgagttc
       61 gcggatgcgg gggttgtccg ccgtggcgac gacagcaacc atcggcgagg gtgcagtttt
      121 gtgggggggc ggcgcggtcg ccggcggggc atcgcccagg gcgtgggcaa agcggttttc
      181 cttgctgccg ggagcgcggg gcaattcgac cacgtaggga tcgggtcggc gcgcggcgag
      241 ggcatccagc acgcggcgga cctcggcggg cgaagcaaag gtatgcagcc gcgcggtgcg
      301 tccgcgcagc tcgccggggg tctgcggacc gcgcacgagc agctcgcaca ggacggccag
      361 ttcgggggtg tccacggcga gggtttccag cagccggtgg gtgtacttca gcacgcgcga
      421 gccaacgccg ctgaaggtgc cgacgagctg cttgtcgtat tgcagcgtgg ccaaggcggc
      481 ttcaatctcg ctggtggcga gctgcaggac gggatcacgg ttgtttttct ggttgcaggc
      541 ggccacaagc gagttgagcg tcagcggata gtattcgggg gtggagagtt ctttttccac
      601 cagcgcgccg aggatacgcg cttccaaggg cgtgaggttc agttgcatgc ggccagtata
      661 accatggggc cggtgcggcg caaggcgacg cgagtattct aaaaagtgat gccgatggtg
      721 ccgaggagcg attggaggcg gaaggaaaat tccttatcga agcgctcctg ccgggcgagg
      781 atatccgcgg ggtcggtggc gctggaggac acggtgccgt cggtctcgat gaattgtgct
      841 tgcagcccga tggtcagggc cacgcggttg agcggaattt ccacctgccc gcgaatccgc
      901 aggtttttgt ggctgaaatc cagatttttt tcgcgatggt cggcggtggt gaaccagccg
      961 gtgccgccgt catagctggc aaacaggtat tgcacgcgca ggcgcacctg cacgccctcg
     1021 gtttggccga ggtgcaggtc cacgccgggg ccccactgcg gggcgcccag gatggtcagg
     1081 ccgctggttt ggccgccgac cacgaattct tccggtgggt tttgataggt cttggtgccg
     1141 cggggttcgc cggcatcaat ttcatagcgc ccgccaaaca gcaccagccc gaacgcccag
     1201 gaggggttga tgcgcacgaa gtcgccgatg ttgaagccca cgggcacgag caggaagttg
     1261 agttcggtca tgttgccgaa caactcggcg tggaagggcg cgccggcggg aatcatcagg
     1321 tggtcgtagc tctggccccg atattcgatg ctgtcgctga tggcgatata gtaatcgcgc
     1381 cgggcggtgg cggatgtggt ggggttcagg aaaatggaat ccagttggag ctgcacaaag
     1441 gcccaggcca tatcgagcga gaagcccatg gcgcggtagg agccatcgag gttgaagtcg
     1501 ctggtgctgt agatttcggc atcgtcctgc ttccaggtgc tgccggtgac atcgtagagc
     1561 ttgcggcggg tttcggcgat catgccctcg aattcggaca ccacgccgaa cgagaggcgc
     1621 aggttttggt cgcggccggg ttcaaggcag ccgccccatt ccacgccggc gcacagcgcg
     1681 cccgtcccgg tggtcaaggt gagcagcgtg gcaagcagga tttggcggca ggtcatgtga
     1741 tccttggggt tgaggtgtgt tttttggggg cgcttagtcg cgcagcagca gccactggtt
     1801 cagccaccgg gtgtagcggc catcggcctt ggcggcggcc agcgcggcat tgacggtggt
     1861 gagcaaatcc gcgcggccgg tgcgcacggc cacgccgtag tggtcgggcg ggaagtccag
     1921 ttgcgtgagg gtgatttcgg gatcgccttg cacgagggcg cgggcgggtt gttcgtcgag
     1981 cacgatggca tccgtgccgc tgtttttgag caggatgacg gcatcccaca gcgcgcccat
     2041 cagcagcacg ttttccatgc tggtgaggtc gccggccaac gcggcgccgg cggagccggg
     2101 cagtgcggcg agccgcttgt cgcgcaggtc ttcggcggtt tggggcgcgg gctgtccggc
     2161 cagcgtaatg acgacagggg tggcgttgta gtacggctcg gaaaaatcgg cggcctgccg
     2221 gcgctccggc gtgatgctca tcgcggcgac cacaaagtcc acctggtcgg cggccaacgc
     2281 cggaagcagg tccacaaaat caagttcctt gatgagcagg gggcggccgg cttgcgcggc
     2341 aatggccttg gcgatttcga tatcgaagcc ggcgaagccg ccttcggcgg tatcgctgcg
     2401 ggaggcaaac gggggcagtt tggcatcggt gccaagcacc agcggcggcg tggtggtgcg
     2461 ggagctgcag gcggccaacc accccgcgac gagagccagc aacgccagac gaaacaactt
     2521 gattttcatg gtgtactccc tgctgaaaac atagggtcag cttaccgtgc cggccaacgg
     2581 gttgtaaatg gcattccccc gccgcggcgg ggaatcaagg cgcggcgggt gcggcttcgt
     2641 tctggaatcc ggcggcggcc gcgcgggcgg cggtggcgga ggcgcagtcg gcaatttgca
     2701 tggccagcag gcgtacgggg ctgccgcgct ccacgccgag aatgccgcca tccaccgcga
     2761 accagcgttc gccgccgccg ggggtgcgca catggaccac gccggctttc agcccggcca
     2821 gcagcggcaa gtgcccggcc agcacaccca gctcgccatt caacgcgggc acggtgatgc
     2881 tttcggcagg accggaaaaa cattcccgat cgggcgtgcg aatgaccagc gggaattcgg
     2941 tcatgactgc gctttcatgg tttgcgcctt ggccaagacg tcatcaatgg agccgaccat
     3001 atagaaggcc tgttcgggga cgtcgtcatg ctgcccggcg aggacttcct tgaaggagcg
     3061 gatggtctgc tccagtggca cgaaacggcc gggaatgttg acgaattgct cggcgacaaa
     3121 aaagggctgg gacagcaggc gctgcacgcg gcgcgcgcgg gcgaccacgg cgcggtcttc
     3181 gtcggagagt tcatccatgc ccaggatggc gatgatgtcc tgcaaatcct tgtagcgctg
     3241 gaggatttcc ttgacgccct gcgccacgcg gtaatgctcc tcgcccacga catagggatt
     3301 gagcaggctg gaggaggacg tgagggggtc cacggcggga taaatcccca gctcgaccat
     3361 ggcgcgggag agctcggtgg tggcatcgag gtgcacgaag gtggtggcgg gggcggggtc
     3421 ggtatagtca tccgccggca catagacggc ctggatcgag gtgatggagc cctgcttggt
     3481 ggaggtgatg cgctcttgca gctcgcccat gtcggtgccg agggtgggct ggtagcccac
     3541 ggccgaagga atgcgcccga gcatggcgga cacttccgcg ccggcctgcg tgaagcggta
     3601 gatgttgtca atgaacagca ggacatcctg ctgcatttcg tcgcggaaat attcggcgac
     3661 ggtcagggcg ctgagcgcga cgcgggcgcg cgcgccgggg ggctcgttca tctggccata
     3721 gaccagcacg gttttgtcaa tcacgccgga gtccttcatc tcctgataca ggtcagttcc
     3781 ttcgcgcgtg cgttcgccca cgccggcaaa caccgagtag ccgccgtagg tcttggcgat
     3841 gttgtggatc agctcctgga tcagcaccgt tttgcccacg cccgcgccgc cgaacaagcc
     3901 gatcttgccg cccttgcggt atggcgtcag caagtccacc accttgatgc cggtgacgag
     3961 gatgttttcc tgcgtgcttt gatcggtgaa ggccggcgtg gggcggtgaa tcggcaggcg
     4021 cttggcggcc gtgatgggcc ccccgccatc caccggttcg ccgagcaggt tcatgacgcg
     4081 cgccaaggtg ccggggccca cgggcacttc gatttcgcgc ccggtatcgc ggacttccgc
     4141 gccgcgcacg aggccatcgg tctggtgcat ggcaatggcg cgcacgaggc tgtcgccgag
     4201 gtgctgggct acttccagca cgaggttgtc cggctggtcg ttaatggcgg cattggtggt
     4261 ggtgagcgcg tggagaatgg gcggcagatc gccggggggg aattccacat ccaccacggc
     4321 gcccatgacc tgggtgatat ggccgaggcg cggtttggcg gggggggtgg cggttggttt
     4381 catgatcgtt ttccttcggt tgggcgtggg ttcaatgcag ggcctccgcg ccggcgacaa
     4441 cttcggttaa ttccgtggtg atggcatcct gccgcgcgcg gttgcgcagc agagtatagg
     4501 tctggcacaa gtgatcaatg ttcttggccg cgctatccat ggcggtcatg cgcgcggcgt
     4561 gttcgccgct ggcgttttcc cacagcgcgg aaaagatgcg ctggcaaacg tgcagctcca
     4621 ggacgtgggt cagcagccgg ggcgaatgtg gctccagcag cacatcgggc tcgcgcccgg
     4681 cgggcgcggg gggcagcgcg gagaggtcca cgggcagcag ccgctcgacc acgggttcct
     4741 gccagagggg gctgaagtag cggttgcggg ccaggtagat ttcgtcgtac ttgccttcca
     4801 cgtagggggt gaacacctcg cggctcagcc ggcgggccag cgtcaggtcc gggtggcgca
     4861 cggcctgctc gtagaacgcg cgcacttcca cctgccggcg aaaatgctgg tagcccttgc
     4921 gtccgcaaaa actgaagcgc aggatgcggt agcgcgcggc gttttcgtcg agccagcggc
     4981 ccaccagccg gatcaggttg ttgttgaacc cgccgcagag ccccatgtcg gccgtcagca
     5041 tcagcacaaa cacgttttcc ggcttggcgc ggggcgccac cagcggattc tgccggttga
     5101 ccagatgttc cgccatgcgc agcagatgcc gccattccgt gccgaacccc tgcgcctgcg
     5161 tcagcatggc ctgggcgcgc gccagcttgg tggtggccag cattttcatg gcgccggtca
     5221 cccggcgcat tccctgcaac tgccggagct tggcgttgta ttcccggtaa ttggccatgg
     5281 cgcggcgcgg ttctcctcaa gccaccacga atgttttgtt gaacgcggcc agcatggcat
     5341 cgagctccgc cttgaggtcg ctggtcaagt cgcccgtctg ttcgaggcgt tcgcggagcg
     5401 cggcgtgggg cccggtgagc gcgaggccga gttgccgttc atattcggcc atctgcggca
     5461 cggggatgtc gtcgcacgcg ccggtgctgc aagcgtagag aaagatgatt tgctggctaa
     5521 ccggtaatgg acgttggggg ccctgcttga aggcttccat caggcgcttg ccgcgcgcca
     5581 attgctggag agtggctttg tccagatcgc ccgcgaactg ggcaaacgcc gccatttcac
     5641 ggtattgcgc cagcgtcagg cgcagcgtgc cggccacttt tttcatggcc ttgagctggg
     5701 catcgccgcc cacgcgggaa acggaaatgc ccggattgat ggccgggcgc tggccggcgt
     5761 tgaaggcgct ggactccagg aaaatctggc cgtcagtgat cgaaatcacg ttggtgggga
     5821 tgtaggccga aatgtcgttg gcctgggttt cgacaatcgg cagggcggtc aagctgccgc
     5881 caccgcgcgc gggactcact ttggccgcgc gctccagcaa ccggctgtgc agatagaaga
     5941 tgtcacccgg ataggcctcg cgccccggcg ggcggcgcag cagcagcgag agctggcggt
     6001 aggcctgcgc ctgcttggtc aagtcgtcat acacaatcag cgcgtggccg ccgcggtcgc
     6061 gccaatattc cgccatggcg cagccggcaa acggcgcgag atattgcagg ggggcggggt
     6121 cggctgccgt ggcggccaca atggtggtat aggccatggc gccgtgctgg cgcagctttt
     6181 cgcacacctg cgtgacggtc gaacgcttct ggccaatggc cacataaaaa caatgcacgt
     6241 ttttgtcggc ctggttgatg atggtgtcaa tgcccagcgt ggttttgccg gttttgcggt
     6301 cgccgatgat caactcgcgc tggccgcggc cgatgggcgt caaggcgtca atcaccttga
     6361 tgccggtttg gaggggctcg cgcacttttt ggcggtcgag gatgcccggc gcgggcgctt
     6421 ccagcgggcg caattcggcg gcgtccggcg ggggaccgcc atccagcgga ttgccgagcg
     6481 catccaccac gcgcccggcc agcgcggggc ccacgggcac ggcggcaatc tgccgtgtcc
     6541 ggcgcaccaa gtcgccctcc cggatgccgg cggcatcgcc catgatggcc acgcccacgc
     6601 tttgttcttc caggttaagc acaatgccgc gcacgccgtt ggtgaactcc accagctcgc
     6661 cagccatggc ctgctgcaag ccgaaaatgc gcgcgatgcc atcgcccaca ctgagcaccg
     6721 tgccgctttc gaacacctcg gcggcgcggt caaagttttc aatctgccgc ttgaggatgg
     6781 cggatatttc tgcgggtttg agctccacgg tcatggcaca acactccttc aacctgccgt
     6841 caaccgacgg cgcagccgcc gcaaccggcc ggccacacta taatcatgca cttcgtcacc
     6901 gatgtaggcg ataaaaccac cgcgcagctc cggggcttcg cggaaaaccg gcgccagtcg
     6961 ccggccggcg cccagtcgcg ccagcagcac ttcgcgctgg gcgccggtca ggggccgggc
     7021 ggagacaatt tccgccggca tcacgcgggc ctgcgcctgc tgccggcgcg caaattcggc
     7081 gagaatccgc ggcaaatgtt ccagcaattg cgcgccttcg cactgcgcca aaaactcggt
     7141 catggtgggg ctaaatgccg cgcgctgcgc cagctcgcgc aaggcggcca ggcgcgccgg
     7201 tttttcgccc agatgccggc aagccacgaa cgtccggaaa tccggtgtag tctgcagcca
     7261 cgcctgcact gtttccacct ccgccttcac gcgggccagc gaatcgttct gcgtggccag
     7321 gtccaggagg gccttggcgt agcggcgaat aatggccagc ggttgcgcca cgtcactcca
     7381 gctcctgcaa gaggcggtcg gtcagcgcgt ggtcggcggg ggcatccagc ttctgatcca
     7441 gcacgcgccc ggccacatcc agcgccaatt ccgccgcggt gcgccgcagt tcgcccaccg
     7501 cctgctcctg cgcgcggcga atggcgtctt gcgcggcgcg aacggcctgc gcggcatccg
     7561 ccgcggcttt ttcctccacc gccttggcgg tggcagccgc cgcttcctgc gcgcgggcca
     7621 ccagcgcagc cgcttcctgc ttggcgttgg ccaacatgac ccgctgagcc tctttatcca
     7681 gctccgcctg cgcgcggatg tcttccgccg cctgcaggct ttggcggatt ttatcctcgc
     7741 gccggtccag cgcctggcag atgggcttcc acgccagttt gtaaagtgcg aagcaggcca
     7801 gcaggaacgc cacccaggtc aacatgacca tttggtcgct gacctgcagg acgttgccga
     7861 tctccgtcgc cggcgccggc acggccacca tctcctgtgt ttcgttcatg cgcgcgcgcg
     7921 tccgatcgcc tggcctagat ctgcaggaag cagatcacca gcgcgaacag cgcgatgcct
     7981 tccaccagcg ccgcgccgat gaacatcgaa gtttgcagct tcgccgccag ttccggttgc
     8041 cgggcgatgc ccttgaggcc cgccgaccat acaaaaccaa tgcccacgcc cgcgccaatg
     8101 gccacgaggc ccgcgcccaa gctggccaat gccagcccct tgaatgccaa ttccgtcatg
     8161 cttcagctcc ttgtctttcc ttatgggttg tccacaccgg tttgcgctca atgcccttca
     8221 tacgccattt ggccaatgaa gatcgcggcc aagagcgaaa aaatgtacgc ctgcaaaaag
     8281 gacatgaaaa tctcaaagaa ataaatcccg accaccagca agatgatggg cgaggccagc
     8341 cagccgaaca gcaccaccat gctcagcagc gcaaaaatca ggatgtgccc cgccagcacg
     8401 ttggcaaaca accggatggc cagcgcgaac acgcgcgaga agaagctgat gacctccacc
     8461 ggcaccagca ccggcagcag aatccacggc gcgccggtgg gcacaaacgt ccgcagaaaa
     8521 ccccgcacgc ccttttgcca gatggtcacc cccaccatga acgcgccggc caccagcgcc
     8581 aggccgcccg tgacgttgat gttgctggtg gccgcgctga acaacggaca aagtcccagc
     8641 aggttcagcg tcaggatcag gaaaaaaaac gcgcaaaaca ccggcgcgaa ccggcgcgca
     8701 tcccgctcgg gaaaataagg ctgcacaatc tggttacgca caaacaccac gaacaattcc
     8761 agcaaattgc tccagccgcg cggcacgtca ccctgctggc gacgatacaa aaacgcaaag
     8821 ccggccacca gcagcagcag ggccagcgcc agcatcacgc catcgttgcg gaacacgtcc
     8881 agcgcctgca cgcggatgaa cggcaggttc cacgcgctgg ggttgccatc ggccacgtgg
     8941 tacagcacgt ggtgctggat gtagtggtgg acgatgtcct tgcggtcggg cgccgcaaca
     9001 acgccggcca ccacattggt cacggccggc aggaccatct ccagcgccgg ttccatttag
     9061 cgggggtcct gattgcccgc atcccggcgc agcactttgc gaatctcata cgccactgcc
     9121 cccatgcccg ccagccaccc caccaccacc cccgccagcc gcgcccccag cagcccgccg
     9181 atccaatagc ccagccccag atagaccgcc agcagcaccg ggatggtcca agccaagccg
     9241 atggcttgct gcccccccgc aaagaatgga gattgcccgg tcatgccagt tcactcatgc
     9301 ggaaaatact gctcccccct accccgtaga acaagaaaaa atcacac
//
LOCUS       DAOPNP010000097         9277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8402_length_9277_cov_9.398829, whole genome shotgun sequence.
ACCESSION   DAOPNP010000097 DAOPNP010000000
VERSION     DAOPNP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8402_length_9277_cov_9.398829"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..645)
                     /gene="tadA"
                     /locus_tag="PKI64_08615"
     CDS             complement(139..645)
                     /gene="tadA"
                     /locus_tag="PKI64_08615"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOE01105.1"
                     /translation="MNDAPSMVVHEARMRLALRQARAAAAAGEVPVGAVIYNSGQLAG
                     QAWNQTQTLKDPTAHAEILAITQAASAAGDWRLTDSILYVTKEPCPMCAGAIVLARIP
                     LVVWGATDPLRGGAVSRFQILQTAELNHRPQIITGVLADECAALLKDFFRQRRLENKQ
                     ENAADDAE"
     gene            complement(642..1547)
                     /locus_tag="PKI64_08620"
     CDS             complement(642..1547)
                     /locus_tag="PKI64_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01106.1"
                     /translation="MKYKLSHVLEYAAARAVSALLVLLPHRVTLALVWGPVRLAWVLK
                     PAFRARTLRRLHQALGPGKTEAELRRIAWLAFRNLAFTAVESIRFPGTSRTWVERHMK
                     LGNVDDVHAALATGRGFIAAVPHMGNWELAGIALHSLGVELVTLARRQKNPLMDAWIN
                     RLRMSTGVEAIDTRSRAIAEIPRKLLEEKKVLALLPDVRSRVGGVPVRFFGVEIEVPG
                     GAAHYARATGVPVFTAEVTRTGWARHGWRKTGRIESDPALSEAEDYRRIMQYILTRFE
                     ESVRANPENYFWFNKRWVLGEEPRP"
     gene            complement(1544..>1936)
                     /locus_tag="PKI64_08625"
     CDS             complement(1544..>1936)
                     /locus_tag="PKI64_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE01107.1"
                     /translation="WLSRASGRIVRLPTEAEWEWAARGGVRGAPYPWGWGGDPRRLAQ
                     FATNAPARRGGLFPANGFGLYDMAGNLYEWCALAAAEPATRRVARGGSWAEQDPTRLE
                     NGHRQPFAADYSGRDVGFRVLREWPVTE"
     assembly_gap    1937..2036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2170..3414
                     /locus_tag="PKI64_08630"
     CDS             2170..3414
                     /locus_tag="PKI64_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01108.1"
                     /translation="MEQRKSKSSKYGMFTGICRIIGLGIILGVALGIPFKTDDGWSQS
                     ELAALAATGIALAYVILAAIDWALQRLALGKNPLPDDLDLADRAVARERCQALNGNSL
                     LQRHCRRLLAAWAAGASGPQVTMMAANQTFRMLGLLAAEPLAILALLFATAGFQAPGP
                     LLTLGTGLMVLVALAGLARFQLAAHAAGYIESNLLARIGNDTPAAAAADFTKNAAKAV
                     ADSMDSLAAAQAKFAEQLTQAQQQATAQLAKAQEAAAAQIAKAQQDAAAQVAKAQEAA
                     STQIAKAQQDAAAQVAKAQQEAAAQAAQAQEAAAAQIAKTHSDVAAKLSATQQETGAQ
                     LAKAQDKVAEQLGRVTDVAASVDQVLKLQQAVDGTLKGVTVTDEFKSTLLELKRHLAE
                     SDQLLRNAAKPRTIRLVEKDNE"
     gene            3428..4168
                     /gene="rsmI"
                     /locus_tag="PKI64_08635"
     CDS             3428..4168
                     /gene="rsmI"
                     /locus_tag="PKI64_08635"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOE01109.1"
                     /translation="MAAESAPAPAGEPGAASSEPLPPGLYLVGTPIGNLEDITLRALR
                     VLRGVTLILAEDTRHSRRLLDRYGITTPLLSYHKFNERARADDILRRIRAGESLALIT
                     DAGMPGISDPGARAAVAVRAAGLYVTVIPGPSALSAAAALCGATRHDGFVFAGFLDHK
                     TAARRRRLRELAGGGTPVVIYESTHRILKLFAEVAEELGPDRRLFVARELTKKHEETV
                     VGTPAELQAHYATHSRKGEFVVILLPAD"
     gene            4219..5091
                     /gene="aroE"
                     /locus_tag="PKI64_08640"
     CDS             4219..5091
                     /gene="aroE"
                     /locus_tag="PKI64_08640"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOE01110.1"
                     /translation="MKTARVVPYALLGHPVAHSLSPAMHNAAFKALRLPAHYSLLDVA
                     PGDIPRTLQELQTRQYGGLNITIPHKEAAYQFLAANGQLTATARLLRAVNTVVFRRNG
                     VMLGDNTDAPGFLDALQESFRTTPRNKTVLLLGCGGAGRALGLICALRGAKAIYLADL
                     NLAARRRLRLAIQRAAPTVPVTCVSLDRARRVAADCTLIIHATPVGMHAGDSSLLPPE
                     SFRPGQIVFDLIYNPAVTPLLATAQAAGARTANGLGMLLHQGARAFRQWTGRRPPVAV
                     MRRALLKALKARTP"
     assembly_gap    5186..5285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5286..5738
                     /locus_tag="PKI64_08645"
     CDS             <5286..5738
                     /locus_tag="PKI64_08645"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOE01111.1"
                     /translation="DRVTLGGMAAGLVLSAAFPTLQGQADVLSALRESVLGLVVGYGI
                     LWLVAVVGRLVLRRDAMGLGDVKLLGAIGACLGWPAVLFTIFVSSLTGTLLGLGLIAA
                     RRKDLQSKIPYGPHLALGATLWMFCGPVCVDWYLSLASAAGRAATLPP"
     gene            5748..6860
                     /locus_tag="PKI64_08650"
     CDS             5748..6860
                     /locus_tag="PKI64_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE01112.1"
                     /translation="MPEPQPLLRPRNLSASALSLGILALIAVGTVLKLAQSVVVPLLI
                     AWLLSYLLAPVVTFLVRRLRFPSFLAVASVVVLLLLAAWGAGLVIQARLLEFARVFPE
                     YQKQLAALTDTYGKLLDLPTAVLKEADWTAQLGPLVLRTSKFFLAFIANAILVLIFLV
                     FMLLAKPYANDKLRAALPDRADGLIALTNTISRQIGTYLGTLFVISLLTGLCVWGALA
                     ALGVEFSFTWGLLAFVLNFIPNIGSIVASIPPVLIALVQFAPALWPAVMTALTLLVLH
                     QVSGNLIAPKLYGERLNLSPVVILLFLLFWGWLWGITGALLAVPIAAAIQITLAHIPA
                     LAPLAVLMGSGRRYARAAPPPPPAAPDAPPAPPTEG"
     gene            complement(6857..8158)
                     /gene="purB"
                     /locus_tag="PKI64_08655"
     CDS             complement(6857..8158)
                     /gene="purB"
                     /locus_tag="PKI64_08655"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOE01113.1"
                     /translation="MIARYTRPEMGALWTDEHKFQTWLKVELAVAAAQAKSGEIPARE
                     YGVIRRKARCDVARIREIEAEVRHDVIAFLTNVAENVGPAARYLHQGLTSSDVVDTAL
                     AVNLVRALDLLVAGVGKVRAAVARVARRHAFTPMIGRSHGIHAEPTTFGLKMLLMYDE
                     FGRALARLQTARETVRVGKLSGAVGTHAHVSAKVEAAVCRQLGLRPAAVSTQILQRDR
                     HAELVGTLALVGASVERWAQEFRHLQRTEVREVEEFFGRRQKGSSAMPHKRNPIVAEQ
                     LCGLARVLRGHAVTALENVALWHERDISHSSAERIILPDATILLDYMLDKLAALVAGL
                     QVDARRMQENLDSTHGLIHSQQVLLALVAKGLAREEAYRVVQGAAMVAWRTGQPLAEL
                     LAADAVVAARLTPRELAACFDVRRHFRDVRRTFRAAGLPLA"
     gene            complement(8239..9195)
                     /locus_tag="PKI64_08660"
     CDS             complement(8239..9195)
                     /locus_tag="PKI64_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01114.1"
                     /translation="MDKKTDDIEKTPATEQGAAPARPSEDMQQFSKWMAEYGKPALTG
                     LVLAAVILAGISVWRGRQASKAEAAVQALFQSQSPEEFQQLALRDPQAATAPLALASA
                     AAEYYAQGRYDEALAAYEHFLTAYPSNMLAPEAKYGVAACQEALDEYAAAVAGYQAFA
                     ADYPENPQRPQALFAAARCLEQMESFAEARAVYEDFLAENPDSAWQEQAESGLLFLKK
                     TERAKQAAASAPAAEETVTEAATPPVAVEKKATKPKATTKKPKAAAKAKEAEEPQAAA
                     EAQVTDEPQADEPAPAVEPPKKKSAGKKKAAAAEPAAEAATD"
BASE COUNT         1425 a         3345 c         2667 g         1640 t
ORIGIN      
        1 ctacaatcca accaagatcg gtttcgggga ctgggacacc ccaaaatgaa attggctgct
       61 ggcaatgcaa cgagccacaa cgagattttc ggggagcgcg ggactcagga cgagcgccgc
      121 tacgagccga agtagcgcct actcggcatc gtccgccgcg ttctcctgtt tgttctccaa
      181 ccggcgctgc cggaaaaaat ctttgagcaa tgcggcgcat tcgtcggcca gcacgcccgt
      241 gatgatttgc ggccggtgat tcagctccgc cgtttggaga atttggaagc gcgacaccgc
      301 gccaccgcgc agcgggtcgg tggcgcccca caccaccagc ggaatccgcg ccagcacgat
      361 ggcgccggcg cacatggggc aaggttcctt ggtcacgtag agaatgctgt cggtcaagcg
      421 ccaatcgccg gcggcgctgg ccgcctgcgt gatggccaga atctccgcat gggcggtggg
      481 gtccttgagc gtttgcgttt ggttccacgc ctgccccgcg agttggccgc tgttatagat
      541 gaccgcgccc acgggcactt cgccggcggc cgccgccgcg cgggcctgcc gcaacgcgag
      601 ccgcatccgc gcttcatgca cgaccatgct gggggcgtcg ttcacggtcg tggctcctcg
      661 cccagcaccc agcgcttgtt gaaccagaaa tagttttccg gattcgcgcg cacggattct
      721 tcaaagcgcg tcaggatgta ttgcatgatg cgccggtagt cttcggcttc ggaaagcgca
      781 ggatcgcttt cgatgcgccc ggttttccgc cagccgtgcc gtgcccagcc ggtgcgggtg
      841 acttccgcgg tgaacaccgg cacaccggta gcgcgggcat aatgcgcggc gccgccgggc
      901 acttcgattt ccacaccaaa aaaccgcacc ggaacaccgc ccacccgcga gcgtacgtcc
      961 ggcaacagcg ccagcacttt tttctcttca agcaacttgc ggggaatttc ggcgatggcg
     1021 cggctgcgtg tgtcaatggc ttccacgccg gtgctcatcc gcagccggtt gatccaggca
     1081 tccatcaacg gatttttctg gcggcgggcc agcgtgacca gttccacgcc cagcgaatgc
     1141 aacgcaatgc cggccagctc ccaattgccc atgtgcggta cagccgcgat gaacccgcgc
     1201 cccgtggcca gcgccgcgtg aacgtcgtcc acgttgccga gcttcatgtg ccgttccacc
     1261 cacgtgcggc tggtgccggg aaagcggatg ctctccacgg cggtgaatgc caaattacga
     1321 aacgccaacc acgcgatgcg ccgcaactcg gcctctgttt ttcccggccc gagcgcttgg
     1381 tgcagccggc gtaacgtccg tgcgcgaaat gccggtttga gcacccatgc cagccgcacc
     1441 ggcccccaca ccaacgccag cgtgacgcga tgcggcaaca gcaccaacag cgcggaaacg
     1501 gcacgggccg cggcatattc caacacatgt gacagtttgt atttcattcc gtaaccggcc
     1561 attcccgcag cacccggaag cccacatcgc gcccgctgta atccgcggca aacggctggc
     1621 ggtggccgtt ctccaagcgg gtggggtctt gctccgccca actgccgccg cgggccacgc
     1681 gccgggtggc gggttcggcg gccgccaacg cgcaccattc atacaagttg ccggccatgt
     1741 cgtacaatcc gaatccattg gcggggaaca aaccgccgcg gcgcgcgggt gcgttggtcg
     1801 caaactgcgc caagcggcgt gggtccccac cccaccccca cggatacggc gcgccccgca
     1861 ccccgccgcg cgcggcccat tcccattcgg cctcagtcgg caagcgcacg atgcggccgc
     1921 tcgcgcgcga caaccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg
     2041 atcagggcca ccgtcagcgc gattcttgtc cgccatttca ttccgcggat taaagcgcat
     2101 cggcctttga caatccaccg tcttttccgc atactggttt gttagtcaat tcagcgaaag
     2161 gcggatgaca tggagcagcg gaagtcgaag agtagcaaat acggcatgtt caccggcatt
     2221 tgccgaatca tcggactggg cattattttg ggcgtggcgc tgggcattcc gttcaaaacg
     2281 gacgatggct ggagccaatc ggaactcgcc gcgttggccg ccaccggcat tgcgctggcc
     2341 tacgtgattc tggccgcgat tgattgggcg ctgcaacggc tggcgctcgg caagaatccg
     2401 ctgccggacg atttggattt ggcggaccgc gccgtcgcgc gcgaacgttg ccaggccctg
     2461 aacggcaatt cgctcctgca acggcattgc cgccggttgc tcgccgcgtg ggcggccggt
     2521 gcctccggcc cacaagtgac catgatggcc gccaaccaaa ctttccggat gctgggcctg
     2581 ctggccgcgg agccgctggc gattctggcc ctgttgtttg ccaccgccgg ttttcaagcg
     2641 ccgggccccc tgctcacgct gggcacgggc ttgatggtcc tggtggcgct ggcgggtttg
     2701 gcgcggttcc agttggccgc gcacgccgcc ggctacattg aatccaacct gctcgcgcgc
     2761 attggcaacg atacgcccgc cgccgccgcc gctgatttca ccaagaacgc cgcgaaggcc
     2821 gtcgcggatt ccatggacag ccttgccgcc gcccaagcca agtttgccga gcagttgacc
     2881 caggcgcagc agcaagccac ggcccagttg gccaaggcgc aggaagcggc ggccgcccag
     2941 atcgccaagg cgcagcagga cgccgccgcg caagtggcca aagcgcagga agcggcatcc
     3001 acgcagatcg ccaaggcgca gcaggacgca gccgcgcaag tggccaaggc ccagcaagag
     3061 gccgccgcgc aagcggcgca agcgcaggaa gccgcggcgg cgcaaattgc caaaacccat
     3121 agcgacgtgg ccgccaagct cagcgccacg cagcaggaaa ccggcgcgca gctcgccaag
     3181 gcgcaggaca aggtggccga gcagttgggc cgcgtgacgg atgtcgccgc ctcggtggac
     3241 caagtgctca agctgcaaca ggcggtggat ggcacgctca agggcgtgac ggtcacggac
     3301 gaattcaagt ccacgctgct ggagctgaag cgccacctgg ccgaatccga ccagttgctg
     3361 cgcaatgccg ccaagccgcg caccatccgt ttggtggaga aggacaacga atagcgtccc
     3421 cgccaacgtg gccgctgaat ccgcccccgc gcctgccggc gaaccggggg cggcttcgtc
     3481 tgaaccgctg ccaccggggc tttatctggt cggcacgccg attggcaacc tcgaggacat
     3541 caccctgcgc gcgctgcgcg tgctccgcgg cgtcacgctc attctggccg aggatacccg
     3601 ccacagccgt cgcctgctgg accgctacgg catcaccacg ccgctgctct cctaccacaa
     3661 atttaacgag cgcgcgcggg ccgacgacat cctgcgccgc atccgcgccg gcgaatccct
     3721 cgccctgatc accgatgccg gaatgcccgg catttccgac cccggcgcgc gcgccgccgt
     3781 cgccgtgcgc gcggcggggc tgtacgtcac cgtcattccc ggccccagcg ccctttccgc
     3841 cgcggcggcg ttgtgtggcg ccacgcggca tgacggcttt gtgttcgccg gatttttgga
     3901 tcacaagacc gccgcccgcc gccgccgctt gcgggagctg gccggcggcg gcacccccgt
     3961 ggtgatctat gaatccaccc accgcatttt gaaactgttt gcggaagtgg ccgaagaact
     4021 cggccccgac cgtcgcctgt tcgtggcccg cgagctaacc aaaaagcacg aggaaaccgt
     4081 ggtcggcacc cccgccgaac tacaggccca ctatgccacc cactcgcgca agggcgagtt
     4141 tgtcgtgatt ttgctgccgg cggactaggc cgcttttcac ttcccctgcc cgccaaaatg
     4201 cgccactctt tgaccaccat gaaaaccgcc cgtgttgttc cgtacgcgct cttgggccac
     4261 cccgtggcgc attctctttc gccggcgatg cacaacgccg cattcaaggc cctgcgcctg
     4321 ccggcgcatt attcgttgct cgatgtcgcg cccggcgaca ttccgcgcac gctgcaagag
     4381 ctgcaaaccc ggcaatacgg cgggctgaac atcaccattc cgcacaagga agctgcctat
     4441 cagtttttgg cggcgaacgg ccaactgacc gccaccgccc gcctgcttcg cgcggtcaac
     4501 accgtcgtct ttcggcgcaa cggcgtcatg ctgggcgaca acaccgacgc ccccggcttc
     4561 ctcgatgcgc tccaagaatc tttccgcacc acgccgcgca ataaaaccgt cctgctgctg
     4621 ggttgcggcg gcgccgggcg cgcgctgggc ctgatctgcg cgttgcgcgg cgccaaggcc
     4681 atttatttgg ccgatctgaa tctcgccgcg cgccgccgcc tgcgcctggc catccagcgc
     4741 gccgcgccga cggtgccggt gacgtgcgtc tcgctggatc gcgcccgccg cgtcgcggcg
     4801 gattgcacgc tgatcatcca cgccacgccc gtcgggatgc acgccggcga ttcttcgctg
     4861 ctgccgcccg agtccttccg ccccggccaa attgtgttcg acctgattta caatcccgcc
     4921 gtcacgccgc tgctggccac cgcccaagcc gccggtgcgc gcaccgccaa tggcctcggc
     4981 atgctgctgc accaaggcgc gcgcgccttc cggcaatgga ctggtcgccg tccgccggtg
     5041 gccgtcatgc gccgggcgct gctcaaggcg ctgaaagccc gcaccccatg atgcccctca
     5101 cgccagccgg cgaagccgtt tggacgctct ttatttttct ggccgggctg tgcctgggca
     5161 gttttctcaa cgtgtgcatt tggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnngaccg cgtcacgctg ggcggcatgg ccgcggggtt ggtgctatcc gccgcattcc
     5341 ccacgctgca agggcaggcc gatgttctct ccgcgctgcg cgaatcggtg ctgggcctcg
     5401 tggtgggtta cggcatcctg tggctggtgg ccgtcgttgg ccgcctcgtg ttgcgccgcg
     5461 acgccatggg tctcggcgat gtcaaactgc tcggcgcgat tggcgcgtgc ctcggctggc
     5521 cggccgtgct cttcactatc tttgtttcgt cgctgaccgg cacgctgctc ggcctggggc
     5581 tgattgccgc gcgccgcaaa gacctgcaaa gcaaaatccc ctacggcccg catctggcgc
     5641 tgggcgccac gctctggatg ttttgcggcc cggtctgtgt tgattggtat ttatcgttgg
     5701 cgtcggccgc tggccgcgcc gccaccctcc ccccctagga aaccgccatg ccagaacccc
     5761 aacctctcct gcgtccccgg aacttgagcg cctcggcgct ttccctcggc atccttgcgc
     5821 tgattgccgt gggcaccgtc ctcaaactgg cccaatccgt cgtagtgccg ttgttgattg
     5881 cgtggctgct gagctacttg ctcgcgccgg tggtgacgtt cctcgtgcgc cgcctgcgtt
     5941 ttccttcatt cctcgcagtg gccagcgtgg tggtgttgct gctgctggcc gcgtggggcg
     6001 ccggcctcgt gattcaggcg cgcctgctgg agttcgcccg cgtgtttccc gaataccaaa
     6061 agcaactcgc cgcgctcacc gacacctacg gcaagctact cgacctgccc accgccgtcc
     6121 tcaaggaggc cgactggacc gcgcaactgg gccccctcgt gctgcgcacc tccaaattct
     6181 ttttggcctt cattgccaac gcgattctgg tgctaatctt tctggtgttc atgctgctgg
     6241 ccaaacccta cgccaacgac aaactgcgcg ccgcgctgcc cgaccgcgcc gatggtctca
     6301 tcgcgctgac caacaccatt tcgcgccaga tcggcaccta cctcggcacg ctgttcgtca
     6361 tttcgctgct gaccggcctt tgcgtctggg gtgcgctggc cgcgctcggc gtggaatttt
     6421 cgttcacgtg ggggttgctg gcgtttgtgc tgaacttcat tcccaacatc ggctccatcg
     6481 tggccagcat tccaccggtg ttgattgcgt tggtgcaatt cgcccccgcg ttatggcccg
     6541 ccgtcatgac cgcgcttacg ctccttgtgt tgcatcaggt ttcaggcaac ctgatcgccc
     6601 ccaaactcta cggcgaacgg ctcaacctct cgcccgtggt gatcttgctc ttcctgctgt
     6661 tttggggttg gctgtgggga atcaccggtg cgctgctggc ggtgccgatt gctgccgcca
     6721 ttcagatcac gctggcccac atccccgcgc tggcgcccct ggctgtgctg atgggctccg
     6781 gccggcgcta tgcccgcgcc gccccgcccc cgccccccgc ggccccggac gcgccgccgg
     6841 ccccgccgac cgagggttaa gccagcggca agcccgccgc gcggaacgtc cgccgcacat
     6901 cgcggaagtg ccgccgcaca tcgaagcatg ccgccagttc gcgcggcgtg agacgcgcgg
     6961 ccaccacggc atccgccgcc agcaattccg ccagcggctg ccccgtgcgc cacgccacca
     7021 tcgccgcgcc ttgcaccacg cggtacgcct cctcccgcgc caaccctttc gccaccagcg
     7081 ccaacagcac ctgctgcgaa tgaatcagcc cgtgcgtcga atccagattc tcctgcatcc
     7141 gccgtgcatc cacctgcaac cccgccacca gcgccgccaa cttgtccagc atgtagtcca
     7201 gcaggatcgt cgcatcgggc aggatgatcc gctcggcgga ggaatgcgaa atatcgcgct
     7261 catgccacag cgccacattc tccaacgccg tcaccgcgtg gccgcgcaac acgcgcgcca
     7321 gcccgcacaa ctgctccgcc acaatcggat tgcgcttgtg gggcatcgcg cttgaaccct
     7381 tttgccgtcg gccaaaaaat tcttccacct cgcgcacttc ggtccgctgc aaatggcgga
     7441 actcctgcgc ccagcgctcc acgctcgccc ccaccagcgc caacgtgccc accagctccg
     7501 cgtgccggtc gcgctggagg atttgggtgg ataccgccgc cgggcgcagc cccagttgcc
     7561 ggcataccgc cgcctccacc ttggcgctca cgtgtgcgtg cgtccccacc gcgcccgaca
     7621 acttgcccac ccgcacggtt tcccgcgccg tttgcagtcg cgccagcgcc cgcccgaact
     7681 cgtcatacat cagcagcatt ttcaacccga acgtcgtcgg ctcggcatgt atgccgtggc
     7741 tccggccgat catcggcgtg aatgcgtgcc gccgcgccac cctcgccacc gccgcgcgga
     7801 ccttgcccac ccccgccacc agcaaatcca gcgcccgcac cagattcacc gccagcgccg
     7861 tatccaccac atccgagctg gtcagcccct ggtgcagata ccgcgctgcc ggcccgacat
     7921 tctccgccac gttcgtcaga aacgcgatga catcatgccg tacctcagcc tcgatctcgc
     7981 ggatgcgcgc cacatcgcag cgcgccttgc ggcggatcac gccgtactcg cgcgcgggaa
     8041 tctcgccgct tttcgcctgc gccgccgcca cggccagctc caccttcaac cacgtctgaa
     8101 acttgtgctc gtccgtccac aaagcgccca tctcgggccg cgtatagcgc gcaatcatgc
     8161 tccacctccc acaccaaacg atacgcgccg gaaccctgac gttttccggc gcgtgttcat
     8221 ccagctcgct cctcgcggct agtctgtggc cgcttcggcc gccggctccg ctgccgccgc
     8281 cttctttttg ccggccgact tcttcttggg cggctccacc gccggcgccg gttcatccgc
     8341 ctgcggctcg tcagtcacct gcgcttcagc ggccgcttgc ggctcctcgg cttcctttgc
     8401 cttggcggca gccttcggct tcttcgtcgt ggccttgggc ttggtcgcct tcttttccac
     8461 cgccactggg ggcgtcgccg cctcagtcac cgtttcttcc gccgccggcg cactggccgc
     8521 ggcctgcttg gcccgctccg tcttcttcaa gaaaagcaac ccggattccg cctgttcctg
     8581 ccacgcgcta tccggatttt ccgccagaaa atcctcgtac accgcccgcg cttccgcgaa
     8641 gctctccatc tgctccagac accgcgccgc tgcaaacagc gcctgcggcc gctgcggatt
     8701 ctccggatag tccgccgcga aagcctgata gcccgccact gccgcggcat actcatccag
     8761 cgcctcttgg cacgccgcca cgccgtactt cgcctccggc gccagcatgt tgctcggata
     8821 cgcggtcaaa aaatgctcat acgccgccag cgcctcatcg tagcgcccct gcgcataata
     8881 ctccgccgcc gcggaagcca acgccagcgg cgcggtcgcc gcctgcggat cacgcaacgc
     8941 caattgctga aactcctccg gactctggct ttggaacaac gcctgcaccg ccgcctcggc
     9001 cttgctggcc tgccgcccgc gccacaccga aatccccgcc agaatcactg ccgccaaaac
     9061 caggccggtc agcgccggtt tgccatactc ggccatccac ttggaaaatt gctgcatgtc
     9121 ctcgctggga cgtgccgggg ccgccccctg ctcggtggcc ggggtcttct cgatatcatc
     9181 cgttttcttg tccatgaccg cttctttcta tgggctagag aatcgggggc ggattctccc
     9241 cactccgcca tcaaaacgca agccaatcgt tgccccc
//
LOCUS       DAOPNP010000098         9177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8499_length_9177_cov_9.135606, whole genome shotgun sequence.
ACCESSION   DAOPNP010000098 DAOPNP010000000
VERSION     DAOPNP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8499_length_9177_cov_9.135606"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKI64_08665"
     CDS             complement(<1..434)
                     /locus_tag="PKI64_08665"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015228.2"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer composed of two alpha (AccA)
                     and two beta (AccD) subunits; one of the two catalytic
                     subunits that can form the acetyl CoA carboxylase enzyme
                     together with a carrier protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOE01115.1"
                     /translation="MNKGASVAAYTLPFEKPLLDLENKIAELRQFSKDQGLTASQDAA
                     RLEAELVETRRTLYATLTPWQKVMVARHPRRPYTRDYIAAFVRDFTELHGDRLLADDL
                     SIVGGMGWIGEHPVMVIGTQKGRDTKSNLACNFGCPYPEGYR"
     gene            complement(427..1524)
                     /locus_tag="PKI64_08670"
     CDS             complement(427..1524)
                     /locus_tag="PKI64_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOE01116.1"
                     /translation="MELQTPPDIYLMKREKSRAGCMTALFAALIVVALAVIFGLVHWF
                     NQRGTEPAEPVEFPVAPIQDEIPPVQLPDIPLPEVKPTAKPDTKSAAKPSAKPAPAPT
                     PAPAPVPEAAAADTTPESAAVEQAPAANTNEAVATLAKARRKLKAGDHAAARTLALQA
                     LAASPGDAATEEFLSELAMPLLASQRPMPEKVEHVVQSGDYLGKLAATYNTPVALIAK
                     ANNIQGATIRIGETLRLLDGNKHIFALTVSKSRNDCVVTLDGNFFKRYRVSTGTNNIT
                     PTGVFKIVDKIEHPPWHKPGSKVIPYGSPDNQLGTHWLAFDQPGYGMHGTWEPDSIGK
                     QSSAGCVRLLNSDIEELYTILPKGTLVTIIE"
     gene            complement(1546..2364)
                     /locus_tag="PKI64_08675"
     CDS             complement(1546..2364)
                     /locus_tag="PKI64_08675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE01117.1"
                     /translation="MNQEFAGYSITRSISGGGMTKLYVAIDAQQTRYVIRVLDSVMAK
                     DRRNRHRFFHGGEVLAKMHTPQRHPNIVPFVKAGYENKTAYMVLEYIESRTLRDLILF
                     REPLLTDNVMIFIRQLANVIQYVHARGYLHLDIKPENILIQPDGRLVLIDFDLALPRK
                     RFFKKLATVSGTTAYVPPETLVNRVADDRADIYAFGICCYEMLTFRKPYEGEKIDQVR
                     AAQIDPKVPPTPIRQLNPNIPVALANIVMKCIAKNPEDRYPDMVLVLRDLNKLS"
     gene            2554..3282
                     /gene="ric"
                     /locus_tag="PKI64_08680"
     CDS             2554..3282
                     /gene="ric"
                     /locus_tag="PKI64_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOE01118.1"
                     /translation="MSALDQQPIGQIVAQDLNTAKVFERHGMDYCCHGHQPLAAACQT
                     HGVALEQVKAELAAAAQTKDALLPDANAMAIDELARYIVATHHVFTKQILPSIATHFT
                     AVVRAHSENHPELPAQQEAFAALHAELDMHLQKEERVLFPYIEAMVRAERAGQPRPVA
                     GFGTVNAPIAAMISEHETAGAILDKLHAETNGFQPPADACNTYRLLFKELGDLEQDLH
                     RHIALENYLLFPKAIALEAKLQPA"
     gene            complement(3289..5103)
                     /locus_tag="PKI64_08685"
     CDS             complement(3289..5103)
                     /locus_tag="PKI64_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOE01119.1"
                     /translation="MAKLVHNYDESKIQTLSALEHIRKRTGMYIGRVGDGSQYDDGIY
                     VLIKEVMDNSVDEFIMGFGQRVDITLDGAKVRVRDYGRGIPLGKVVECVSRINTGAKY
                     NDDVFQFSVGLNGVGTKAVNALSLSFFVQSHRDGQYANARFERGELVKQATGKCPGEP
                     NGTLIEFEPDPQIFGVVTYQDDLLQRRLRLYACLHSGLAIVYNGTEILSENGLTDLVL
                     EEVGEEAIYGPLHHRTPTLEIAFTHTQRFNETFFSFVNGQYTVDGGTHLSAFREGFLK
                     GVNEYANAKFDGDDVRESMVGAIAIRLKEPVFESQTKNKLGNTEIRSDLVAQIRDVVA
                     DLLHRNPDAAQVLVKKIEETQSLRKELQTVKKLARERSKAVSIRIPQLKDCKSHLDPR
                     RRRGLDSMLFITEGQSAAGSITACRNVTTQAVFTLRGKPLNVAELDRAVMYTNEELYN
                     LMRALNIEDALDGLRFQKVIMATDADVDGLHIRNLLLTFFLRFFEPLVLAGHLFILET
                     PLFRVRNKKETRYCYSEAERDAAMAGLRGAEVTRFKGLGEISPDEFKHFIGPQMRLTP
                     VQVAHDHKIANLLRFYMGRNTPERRQFIMDHLRVTAEE"
     gene            complement(5173..6162)
                     /gene="pdxA"
                     /locus_tag="PKI64_08690"
     CDS             complement(5173..6162)
                     /gene="pdxA"
                     /locus_tag="PKI64_08690"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOE01120.1"
                     /translation="MKPIAIGITCGDANGIGPEVALRAARQPQPANRRIVLIGHRTVW
                     ERTARLIGCPLPPEISALGGPLPRCSTWDPAMAPLPAHRPGRVRADSARAAYAAILAA
                     TAAAQNRQLAAIVTAPISKEAFQRAGIREPGHTELLARLTGTRRFGMMLFGDKIRVIL
                     ATRHLPLRKVADALTADEIVHAVELLARALPWMGFKRARIGVCGLNPHAGDGGALGTE
                     ERDIIAPAIARCRRRGWQVAGPIPADVIFHQHLAGKHDAVVAMYHDQGLAPLKMLSFE
                     NGVNLTLGLPIIRTSPDHGTAFDLAGQGHANPASTRAALDWAARLATRPNPWA"
     gene            complement(6170..7120)
                     /locus_tag="PKI64_08695"
     CDS             complement(6170..7120)
                     /locus_tag="PKI64_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE01121.1"
                     /translation="MKRLAMFTLLLWGCLPAGAQSTPPAGILLDSYAALVNGKVITVG
                     DVLVAMQPAQERLTAQYAGRELERKLQELYTTTREHLVDSELILLEFEKQGGALPDRA
                     LENHVNSVIHDQFNNDRTAFLKALADQRLTYTEWHKQIKEQFIVQLMRQKEVVAKILI
                     TPLDLQQAYDEQKATAFTTPARVQLRTLTLTGAARSQAADLRQRLLAGQLTFAAAAAE
                     DIAAQDEPEWLATDSLAAPLRAALADLAPGDIAEPLELGEATYLVQLVAQEDAHTRPM
                     DDVLPELERQVRNAQFDRLNQAWLESLRAKYYIKLYEHDL"
     gene            7203..8399
                     /locus_tag="PKI64_08700"
     CDS             7203..8399
                     /locus_tag="PKI64_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01122.1"
                     /translation="MNAGVFRRFWLAGVLGALAAQARDIPVHREYTKPITFYDESGQV
                     ARFADVAPDLAAQQSLRDAQAQELLLGREPVLAVAFSEAGLISRRPDHIPGKLSPQTD
                     PQERRVSQRKQAERNWLAGSLSLKNLGQSNSNVATEVFSRGSDSSGWGWLANDVARAG
                     SQQDADSTAKLTEEIMRAEESATAANPSAAMLTGAANPFWREDPALAKVTGPDATGSD
                     AARGTTLQPGNLPTPDVAARPDLMPKTDAVRTAPSALAGDSRGYAPAAAAPDLGQTRQ
                     LLDSFKTESRPDFTALRESLTRGTPPAAPAQAMAPTEPISIKWGGAADRPAPDRGGWS
                     WGQTGTPGASGGLEKPKWGGDWGRPTTGANSWLQHTEQPAVQPVTYPGMTKPKPSSGG
                     LKPGWY"
     gene            8593..>9177
                     /locus_tag="PKI64_08705"
     CDS             8593..>9177
                     /locus_tag="PKI64_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOE01123.1"
                     /translation="MKQSAWGLIIAMGKSEKIASDVETAFLYLNDRPVLAYSLAAFNQ
                     CPEIDGIVVVVSRERAESVLGMVQLFGFAKVRKIVAGGATRAAAMAAGLQHVDDSVEW
                     VCVHDASRPMVKADLIAETVKTARRHGSGIAATEIPDAVVAAKKSAVHARFDQDERLW
                     AAISPQTWPLPALLKAYPKSGKARKNYADDLEAML"
BASE COUNT         1679 a         2771 c         2963 g         1764 t
ORIGIN      
        1 ttgcggtagc cttcgggata ggggcagccg aagttgcagg ccagattgct cttggtatcg
       61 cggccctttt gggtgccgat caccatcacc gggtgttcgc caatccagcc catcccaccc
      121 acgatggaaa ggtcatccgc cagcaggcgg tcgccgtgca gttcggtgaa atcgcgcaca
      181 aaagcggcaa tatagtcgcg ggtgtagggc cggcgcggat ggcgggccac catcactttt
      241 tgccatgggg taagcgtggc gtagagcgtg cggcgtgttt ccaccagctc ggcttccagc
      301 cgggctgcgt cttgcgaggc ggtcagccct tggtctttgg agaattggcg cagctccgca
      361 atcttgtttt ccagatcgag cagcggtttt tcgaagggaa gcgtataggc ggccacggaa
      421 gcacctttat tcaatgatgg tgacgagcgt ccccttgggc aggatggtgt acaattcttc
      481 gatatcgcta ttgagcagcc gcacgcaccc ggcgctggat tgtttgccga tggaatccgg
      541 ctcccacgtg ccgtgcatgc cgtagcccgg ttggtcaaag gccagccagt gcgtgccaag
      601 ctggttgtcc ggcgaaccgt agggaatgac cttgctgccc ggcttgtgcc acggcggatg
      661 ctcgatcttg tccacaatct tgaacacgcc ggtgggcgtg atgttgttgg tgccggtgct
      721 gacccgatag cgcttgaaga agttgccgtc cagggtcacc acgcaatcgt tgcggctctt
      781 gctgacggtg agcgcaaaga tgtgcttgtt gccatcgagc aggcgcaggg tttcgccgat
      841 gcggatcgtg gccccctgga tattgttggc cttggcaatc aatgccacag gcgtgttgta
      901 ggtggccgcc agtttgccga gatagtcgcc gctttgcacc acgtgctcca ctttttccgg
      961 catggggcgc tgcgaagcca gcaggggcat ggcgagttcg ctcaaaaatt cttccgtggc
     1021 ggcatcgccc gggctggcgg ccagggcttg cagcgccaag gtgcgggctg cggcgtgatc
     1081 gccggccttg agtttccggc gggccttggc cagcgtggcg acggcctcgt tggtgttggc
     1141 ggcaggagct tgttctacag cggccgattc cggcgtggta tccgccgccg cggcttccgg
     1201 aacgggcgcg ggggccggcg tgggggccgg ggccggcttg gcactcggct tggctgcgga
     1261 tttggtgtcc ggcttggccg tcggcttgac ttccggcagc ggaatgtccg gcaattgcac
     1321 cggcggaatt tcgtcttgaa ttggcgccac agggaactcg accggctcgg cgggttcggt
     1381 gccgcgctgg ttgaaccaat gcaccaagcc gaaaatgacg gccaacgcca caacgatcaa
     1441 cgcagcgaag agcgccgtca tacacccggc gcgcgacttc tcgcgcttca tcaaatagat
     1501 atcgggaggg gtttgcagtt ccatgggcgg gcgggggcgg gggggttagg atagtttgtt
     1561 gaggtcgcgc agcaccagca ccatgtcggg gtagcggtct tctggattct tggcgatgca
     1621 cttcatcacg atattcgcca gcgccaccgg gatgttgggg ttcagttggc ggatgggcgt
     1681 gggcggcacc ttcggatcaa tttgcgcggc gcggacctgg tcaatttttt cgccttcgta
     1741 gggcttgcgg aaggtcagca tttcgtagca gcaaatgccg aacgcataga tgtcggcccg
     1801 gtcatcggcc acgcggttca ccagcgtttc cggcggcaca taggccgtgg tgccggaaac
     1861 cgtcgccaat tttttgaaga agcgcttgcg gggcagcgcc aggtcaaaat caatcagcac
     1921 gagacggccg tcgggctgga tgagaatgtt ttccggcttg atgtccagat gcaggtagcc
     1981 gcgcgcgtgg acgtattgaa tcacgttggc cagttggcgg atgaaaatca tcacgttatc
     2041 ggtcagcagc ggctcgcgga agaggatcaa atcgcgcagg gtgcgcgact caatatactc
     2101 cagcaccatg taggccgttt tgttttcgta gccggccttg acgaacggca cgatgttcgg
     2161 gtgccgctgg ggggtgtgca tcttggcaag cacttcgccg ccatggaaaa aacggtggcg
     2221 attccgccgg tctttggcca tcacggaatc cagcacgcgg atgacgtagc gcgtttgttg
     2281 cgcgtcaatc gccacgtaca gtttggtcat gccgccgccg gaaatggagc gggtgatcga
     2341 gtagcctgcg aattcttgat tcatgcgagc agttctaccc caaagcgaga agaaacgcaa
     2401 tgccttgttg gcttgccggc gggggggcga aatgtcgagc cagacgtggc tgaaccggtg
     2461 aaaaacaggg caatcatttg gcatttggcc cgcccggggc tatactatcc cgagccggcg
     2521 gcaggccggt ggaaaccttc aggagaaaga accatgtcag cattggacca acaacccatc
     2581 gggcagattg tcgcccaaga cctcaacacg gccaaagtat ttgaacgcca tggcatggat
     2641 tactgttgcc acggccacca gccgctggct gcggcctgcc agacacacgg cgtggcgctg
     2701 gagcaggtga aggccgaact ggccgccgct gcccaaacca aggatgccct gctgcccgat
     2761 gccaacgcca tggcgattga cgagctggcc cgctacatcg tggccaccca ccacgttttc
     2821 acaaaacaga ttttgccctc tatcgccacg cactttacgg cggttgtgcg ggcgcacagc
     2881 gaaaaccatc ccgaactgcc cgcgcagcag gaggcgtttg cggcgttgca cgccgagctg
     2941 gacatgcacc tgcaaaagga agagcgggtg ttgttccctt acatcgaagc catggtgcgg
     3001 gcggagcgcg cgggccagcc gcgccccgtg gcgggattcg gcacggtcaa cgcgcccatc
     3061 gccgccatga tcagcgagca tgaaaccgcc ggtgccatcc tggataaact gcacgcggaa
     3121 accaacggct tccagccacc ggcggatgcc tgcaacacct atcggctgct gttcaaggaa
     3181 ctcggcgatc tggagcagga cctccatcgc cacatcgcgc tggaaaacta tttgctcttc
     3241 cccaaggcca tcgcgctcga ggccaaactg cagcccgcgt aaagcggact attcttccgc
     3301 ggtcacgcgc aggtggtcca tgatgaactg ccggcgctcc ggcgtgttgc ggcccatgta
     3361 gaagcgcagc aggttggcaa tcttgtggtc gtgtgccacc tgcaccggcg tcagccgcat
     3421 ctgtgggccg atgaaatgct tgaactcatc cggcgaaatt tcgccaaggc ccttgaagcg
     3481 ggtgacttcc gcgccacgca accccgccat ggcggcatcg cgttccgctt cgctatagca
     3541 gtagcgcgtt tcctttttgt tgcgcacgcg gaagagcggc gtttccagga tgaacaggtg
     3601 gcccgccagc accagcggct caaaaaagcg caggaaaaat gtcagcagca gattgcggat
     3661 gtgcagcccg tccacgtcgg catcggtggc catgataact ttttggaagc gcaggccgtc
     3721 gagcgcgtcc tcgatgttca gcgcgcgcat caggttgtag agctcctcat tggtgtacat
     3781 gactgcgcgg tcgagttccg caacattgag cggcttgccc cgcagtgtga acaccgcctg
     3841 cgtggtcacg ttgcggcacg cggtgatgga gcccgccgcc gattggcctt cggtgatgaa
     3901 cagcatgctg tccaatcccc gccgccgccg tgggtccaaa tggcttttgc agtccttcaa
     3961 ttgcggaatt cggatgctga ccgccttgga tcgctcgcgg gcgagctttt tgaccgtctg
     4021 caattccttg cgcagcgatt gcgtctcttc gatttttttg accagcactt gcgcggcatc
     4081 gggatttcgg tgcaacaagt cggccaccac gtcgcggatt tgcgccacaa ggtcggaacg
     4141 gatttccgtg ttgcccagct tgtttttggt ctgcgactca aagaccggct ccttcagccg
     4201 gatggcgatg gcgccgacca tggattcccg cacatcgtcg ccatcgaact tcgcgttggc
     4261 atattcattg acgcccttga ggaacccttc gcggaacgcg gagaggtgcg tgccgccatc
     4321 cacggtgtac tgcccattga cgaacgaaaa aaacgtctcg ttgaaccgct gggtatgcgt
     4381 gaaggcgatt tccagcgtgg gcgtccggtg gtgcagcggg ccatagatgg cttcctcgcc
     4441 cacttcttcc agcaccagat ccgtcagtcc gttttcggac aggatttccg tgccgttata
     4501 aacgatggcc agcccggaat gcaggcaggc atagagccgc aggcggcgct ggagcaggtc
     4561 gtcctgatac gtcaccacgc cgaagatttg cgggtcgggc tcgaactcga tcagcgtgcc
     4621 gttgggttcg ccggggcatt tgccggtggc ctgcttgacc agttcgccgc gctcaaaacg
     4681 cgcgttggca tactggccgt cgcggtgtga ttgcacaaaa aacgaaagcg acaacgcatt
     4741 gaccgccttg gtgcccacgc cgttgaggcc cacggaaaac tggaacacat catcgttgta
     4801 tttggcgccg gtgttgatgc gcgagacgca ctccaccacc ttgcccagcg ggatgccgcg
     4861 gccatagtca cgcacgcgca ccttggcgcc gtcgagcgta atgtccacgc gctggccaaa
     4921 ccccatgatg aattcatcca cggagttgtc catgacttcc ttgatcagta catagatccc
     4981 gtcgtcatat tggctgccat cgcccacgcg gccgatatac atgcccgtgc gcttgcggat
     5041 gtgctccagc gccgacagcg tctggatttt gctctcgtcg tagttatgaa ccaatttcgc
     5101 catacgcgcg catcacagca aacgcttccc gaaaaagcaa cccgcagctt ccccggcggg
     5161 cacggttgcg tttcatgccc acgggttggg gcgggtggcc agccgtgcgg cccagtccag
     5221 cgccgcgcgc gtgctggcgg gattggcgtg gccttggccg gccagatcga acgccgtgcc
     5281 gtggtccggg ctggtgcgga tgatcggcag gccaagcgtc aggttcacgc cattttcaaa
     5341 cgacagcatt ttgagcggcg cgaggccctg gtcgtgatac atcgccacca cggcatcatg
     5401 cttgccggcc agatgctggt gaaaaatgac gtcggccgga atcgggccgg caacctgcca
     5461 gccgcgccgg cggcagcggg caatggccgg cgcaatgatg tcgcgttctt ccgtgccgag
     5521 ggcgccgcca tcgccggcgt gcggattcag gccacacacg ccgatgcgcg cgcgcttgaa
     5581 ccccatccac ggcagtgcac gcgcaagcag ttccacggcg tggacaattt catccgcggt
     5641 gagtgcatcg gccactttcc gcagaggaag atggcgcgtg gcaagaatca cccggatttt
     5701 gtcgccaaaa agcatcatgc cgaagcggcg tgtaccggtc agccgtgcca gcagctccgt
     5761 gtggcccggc tcgcggatgc ccgcgcgttg aaaggcttcc ttgctgatgg gcgcagtgac
     5821 gatggccgcc agttggcggt tttgcgcggc ggcggtcgcg gccaaaatgg cggcataggc
     5881 cgcgcgcgcg gaatctgcgc gcacccggcc gggacgatgg gccggcagcg gggccatggc
     5941 cggatcccac gtactgcagc gcggcagcgg tccgccaagt gcggaaattt ccggcggcag
     6001 gggacagcca atcaggcggg ccgtgcgttc ccagaccgtg cggtgcccaa tcagcacgat
     6061 gcgtcggttg gccggttgtg gttgccgcgc cgcgcgcagg gccacttccg ggccaatgcc
     6121 gttcgcgtcg ccgcaagtga tgccgatcgc gatgggcttc atgggccact cacaggtcgt
     6181 gctcgtagag cttgatgtaa tacttggcgc ggagcgactc cagccaagct tgattgagcc
     6241 ggtcgaactg ggcgttgcgt acttggcgtt ccagttcggg cagcacgtcg tccatggggc
     6301 gggtgtgcgc gtcctcttgc gccacgagtt gcacgagata ggtagcctcg cccagttcaa
     6361 gaggctcggc gatgtcgccg ggcgccaagt cggccaacgc ggcgcgcagc ggcgcggcga
     6421 ggctgtcggt ggccagccat tcgggttcat cttgcgcggc aatatcctcg gcagccgccg
     6481 cagcaaaggt caattggccg gctagaaggc gctggcgcag atccgccgcc tgcgaacgcg
     6541 ccgcgccagt cagcgtcaaa gtgcgcaact gcacgcgtgc cggcgtggtg aaggccgtgg
     6601 ctttttgttc gtcataggcc tgctgcaagt cgagcggggt gatgagaatc ttggcgacca
     6661 cttccttttg ccgcatcaac tgaacaatga actgctcttt gatttgcttg tgccattccg
     6721 tataggtcaa ccgttggtcg gccagcgcct tgaggaacgc ggtgcggtcg ttgttaaatt
     6781 gatcgtggat gacggagttc acatggtttt ccagcgcgcg gtcaggcagt gcgccgcctt
     6841 gcttctcaaa ctccagcagg atcagctcgg aatccaccag atgctcgcgg gtggtggtgt
     6901 atagctcttg cagcttgcgc tccagttcgc gcccggcata ttgcgcggtc aggcgttcct
     6961 gcgcgggttg catggccacg aggacatcgc ccacagtgat gaccttgccg ttgaccaatg
     7021 cggcatagct gtccagcaga atgccggccg gtggcgtaga ttgcgccccg gcgggcaggc
     7081 agccccacag cagcagcgtg aacatggcga gtcgtttcat gcgcgggata gtatgccaac
     7141 gcccccggcg acgcaaggtt ccggattgaa tccgcgcgcg gggcggggta ggctgctccg
     7201 caatgaacgc aggtgttttc aggcgattct ggctggccgg cgtgctgggc gcgctggccg
     7261 cccaagcgcg ggatatcccc gtccaccgcg agtacaccaa gcccatcacc ttttatgacg
     7321 aatccggcca ggtggcccgt ttcgccgatg tggcgccgga tttggccgcg caacaatcct
     7381 tgcgcgatgc gcaggcgcag gagttgctcc tcggccgcga accggtgctg gccgtggcct
     7441 tcagcgaggc cgggctgatt tctcgccgcc cggaccacat ccccggcaaa ttgagcccgc
     7501 aaacggatcc gcaggagcgc cgcgtcagcc agcgcaagca agccgagcgc aattggctgg
     7561 ccggcagtct cagtctgaaa aatctggggc aaagcaacag caacgtggcc accgaggtgt
     7621 tctcgcgcgg tagcgattcc tccggctggg gctggctggc caacgatgtc gcccgcgccg
     7681 gcagccagca ggatgcggat tccaccgcca aactgactga ggaaatcatg cgcgcggaag
     7741 aaagtgcaac ggcggccaat ccgtcggcag ccatgctgac gggcgcagcc aatccgtttt
     7801 ggcgcgaaga ccccgcccta gccaaagtta ccggccctga tgccaccggc agcgatgcgg
     7861 ctcgcggaac tacgctccaa cccggcaact tgccaacacc cgacgtggct gcgcgcccag
     7921 acctgatgcc caaaacagat gccgtacgca ccgcaccgag cgccttggcc ggcgattcgc
     7981 gtggttatgc tcccgccgcc gccgcgcccg atctggggca aacccgccaa ttgctcgact
     8041 ccttcaaaac cgaatcacgc ccggatttta ccgccttgcg agagtctttg acccggggca
     8101 ccccgccagc cgctcccgcg caggccatgg ccccaaccga gcccatttca ataaagtggg
     8161 ggggggcggc cgaccgtccc gctcccgacc ggggcggctg gtcatggggc caaactggca
     8221 caccaggggc ctccggaggc ttggaaaagc ccaaatgggg gggggattgg gggcgcccaa
     8281 ccacaggggc taattcttgg ctgcaacata cagaacaacc tgcagtacaa ccggttacat
     8341 atcccggcat gaccaagcca aagccttcca gcggcggcct gaagcccggc tggtattgaa
     8401 ccggcttgtg gccagcgccc gtggcaagcc aaagggcggg ggaggagttg gccaaaagtt
     8461 cagaaaaatc actgtttttg tagagttggg cttgacctcc ccctgccgca tccccaaact
     8521 tacaaatgtg gccgttggat tgcccgctca ccctgcgcgg acctgacggc tgcccaagcg
     8581 aaggaaaaaa ttatgaaaca gtcagcctgg ggtttgatta tcgccatggg gaagtcggag
     8641 aagattgcct ccgatgtgga gacggctttt ttgtatctta acgaccgccc ggtgttggcg
     8701 tactcccttg cggccttcaa ccagtgtccg gaaattgacg gcattgtggt ggtggtcagc
     8761 cgggaacgcg cagagagtgt cttgggcatg gtgcagttgt tcggctttgc caaggtgcgc
     8821 aaaatcgtgg cgggcggcgc aacgcgcgcg gcggcgatgg ccgccggctt gcagcatgtg
     8881 gatgacagcg tcgaatgggt ctgcgtgcac gatgcctcgc gcccgatggt gaaggccgat
     8941 ctgattgccg aaaccgtgaa gaccgcccgg cggcatggct ccggtatcgc cgccacggaa
     9001 atccccgatg cggtcgtcgc cgcgaagaaa agcgccgtcc atgcgcgctt cgatcaggat
     9061 gagcggctgt gggcggccat ctcgccgcag acgtggccgc tgcctgcttt gctgaaggcc
     9121 tatcccaaga gcggcaaggc gcgcaaaaat tacgcggatg atctggaagc gatgctc
//
LOCUS       DAOPNP010000099         9167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8504_length_9167_cov_7.344162, whole genome shotgun sequence.
ACCESSION   DAOPNP010000099 DAOPNP010000000
VERSION     DAOPNP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_9167_cov_7.344162"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1303
                     /locus_tag="PKI64_08710"
     CDS             134..1303
                     /locus_tag="PKI64_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOE01124.1"
                     /translation="MYTGPTIFSQLMQHLPWHHFDRCVSRYGGDHKVKEFKCSSHFRV
                     MSFAQLTCRESLRDIEACLRAMRPKLYHMGIRSTVSRNNLSNANEQRDWRMYAEFAQH
                     LISKARELYAGEELSVELEASVYALDSTTIDLCLSMFPWARFRRHKGAIKMHTLMNLR
                     GNIPEFIHISDGKLHDVNILDYLIPTPGAYYLMDRAYLDFNRLFEINVCRAFFVTRAK
                     SNFKFQRRYSHPVDRNTGLICDQTVLLTNFYPAKAYPEPLRRIKFRDPDDPSSLLVFL
                     SNDFTLPAMTIAKLYKARWMIELFFKWIKQHLRIKAFLGTTPNAVKTQIWIAISTYLL
                     VAILKKRLRIEMELYTILQILSVSLFEKTPILQAFQAWDYKTKTPESHNQLLLFG"
     gene            complement(1419..2609)
                     /locus_tag="PKI64_08715"
     CDS             complement(1419..2609)
                     /locus_tag="PKI64_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOE01125.1"
                     /translation="MTKPLRFTDLPRPNYAGGSLLNLMASLIRGRGGRTPHRALAGLP
                     PAALRNPRKVVVLLLDGLGANQLHRFILAGNGQHFLARHPWQKITTICPATTAAAVTT
                     LATGASPTEHAILGWHLHLPDLGMVGTILPFVTRLDTPIANEEFNIADYLALPSPLAT
                     IPGRRVLISQGYIPTSRTSLAQRWWTERHSFNTLTGFIQKLRAFACSRGRAVAYAYWP
                     HYDSLCHEHGPMGKLPAKHLRELDVFLGRLQRALAGTDTLLLVTADHGHMQTTTHINL
                     AQIPGLYDTQAILPSGDARMVQCFVRPNRVQDFLRLTRQAPLHGAVACVPQATLLRSG
                     ILGPGRPHPALLNRLGDYTLFAVPGHALLYPPAQAKDKKLMPGNHGGLSADELDVPLF
                     VVPC"
     gene            2800..>6208
                     /locus_tag="PKI64_08720"
     CDS             2800..>6208
                     /locus_tag="PKI64_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE01126.1"
                     /translation="MTSWAAEQEGIGSWGVNDPTYGLKTREFEPGFTYTPSPADWRDI
                     NIYQLFTDRFADSGVDQLASYKPGWKTEGKNFPYNRNYHHGGDWKGLKNNIGYLTGMG
                     IKAVWMSGVQMNDQGRDTNYTPYHQYHPENFFKCDPAMGTFAELKELIDALHAAGIYV
                     ILDVAPNHMCDKNGLWGNNQQDDKQYWGGGNNTFGWWDQNNKHPAPFDNLNYFHNNGT
                     INCWDCSPENLLGQFKGTDDLKTEDPAVQSILAKAFKNLIDATDCDGFRVDAIKHVEY
                     NWIKQWADDIRKHAAARGKNDFILFGELFSYDNNALASFCKDLNYSFNSALFFPLSQN
                     IKSVFGDGQNSDQLSNQLNNKYMYGEGADRLVAFIDNHDLNRIALQISGQDVGNAVWK
                     LKPALSFLYLATPVPCLYYGTEHAFNQGGHFNGSNRTEDNPDDGDWQRECMFDKGFQP
                     GPAQGNKLAATNAPLYQHIAALNAARATYKSLTRGSHHTLVTGDAYVFRRLYQGEDSL
                     VGININDGNKSFEVEVIKPQGTEYVNVFNPAEKLIVDQWKKVNFSLGGKETKIFVSVS
                     SESPPLWVENVSHWPVSGEITSDDEVWVNAEAGLQASVSNVTVHYSVNDGAWQTKTMA
                     VNGDWSSQMGSWFHVALGPFPASANVKYKVVLLGKNSTSVTNDNNGANYSFSVAQGSA
                     SGLWVRNAKNYPEDGAATFATEIWIDAEVGPATGVDGVWVIYKVDNGNWTTNVMAVNP
                     DWGSEGGKWYNKNIGTFNEGEVVRYFVRATAGAETVEANNGGTFYQLTIRGAGLAITN
                     PPGNIAVANNVATYTLQGTASAVTNGNIFWTNSLTGASGQFAAATPWTLANVVLGVGA
                     NTITVSGNTGSGSGTILEDKGSNYGGNWTDGSNQGAGFGAWTFNHFQDPPNSYAGTFI
                     GDPAKAGISGFGTNAFGFYANPAGSGANAEVSRTFANPMALGSVFSFKWGLNWDSNAE
                     NSYRGFSLSAGDTELIYINQGNTAAININGQQMFSEYGTQAMVLSFEYVASGSIRVTA
                     NGRNGSENYNELLSVPAGAPSKIKFYYNASDRTPEPEDEDKRQMYVDELKITGAGGGE
                     PLSAVVTITRQDVPVDSNGDGVPDAWLAGHGLPTSTDGGSVAPNGYTY"
     assembly_gap    6209..6308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6309..7847
                     /locus_tag="PKI64_08725"
     CDS             <6309..7847
                     /locus_tag="PKI64_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01127.1"
                     /translation="VRFYDAVAENEHVTVSATPGAGSFTNPAGITVTLHVSGVNVITS
                     TYAVAGGAPAGFTNGQQITIGTGWTNGQSALLTLYGRTKTGITSDKQYRYTYVEPSGV
                     AMTHVGGTHHWVSDEGQVFINSAGYPEGASTEAYVVYCINPPCEGAWPLIPMERIPDW
                     ENGDWWHKDLGIQPNGTEIAFAIMMKDSFGTEYWDNNGTSNYVVTIQSGGGGSGGGDP
                     PPQGGDKPYSTNPTKGKRGTMTIDGSPSGWTDEHLIALDMANDDPRSLGDNWTMHEAP
                     IDLTHMWAAWDDNNLYIAWQYVDVTDIIDPANAGSAGGGKISNNDGILQWIVLDTIAS
                     QGATSDMWAKKSSWTGPNKPDYQIYLAGSLWQGYISRAVGGVFPVDDGGVNYNTIAAA
                     GITVAKGSTFGGTSLWGVGDADHRKNGDAPNRNFLAEGHSTARDSFYEMSIPLSYLNI
                     TAAQLDSGAGVGIMIGGGSTSSMDSIPHDETTLDTPGVESYNSSFEWGDSDIFTTPFA
                     RIGH"
     gene            complement(7964..8200)
                     /locus_tag="PKI64_08730"
     CDS             complement(7964..8200)
                     /locus_tag="PKI64_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01128.1"
                     /translation="MKWMLRTVLLLCGLGFASGCGCGHQTTQDPNDPGRAVLDESRRL
                     HDRATKEHLIGFDDRGFPIYGVDAEGNPVYKRDK"
     gene            8325..9137
                     /locus_tag="PKI64_08735"
     CDS             8325..9137
                     /locus_tag="PKI64_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOE01129.1"
                     /translation="MDSQNAQQGNSENPAPEKMVVVVDEPLMPPFWGAQVWEPTIEEV
                     YPHIERLSLLVGRWGFKKGNLPEAEYRQILEGEAQSHFERLKRENLERRLLVPRAAVA
                     WHTCRPEGDSLIMTAHGTGKTYVQNFPRQQINEKLCLSDFYRKEGDILGCFTVTVGSA
                     EATAEVLRMKEDGEYQNYFFWMGFAAEYADAVAEWAHRKMLEPLGGRKGARFGPGYPS
                     CPDTGLSRTIAELTEAWRIGVTVTDSNMLEPEMSTSAVMAHRPRARIFNRLV"
BASE COUNT         2099 a         2486 c         2707 g         1775 t
ORIGIN      
        1 cagtagtgtc ccaacgtgta agcaaaccat cttcgcaaca tgttatttat gagcatgtta
       61 agaagtgaaa tggctgttgt cgacttgaat ttgcctgtac gcttttacct tccgaaatag
      121 gaaggagacc cggatgtaca caggcccaac aatattctct cagcttatgc aacatttgcc
      181 gtggcatcat tttgaccgat gcgtaagtcg atacggcggc gaccacaagg ttaaggagtt
      241 taagtgttct tcccatttcc gcgtgatgtc cttcgcgcag ttgacctgca gggagagttt
      301 gcgcgacatc gaagcatgtc ttcgggccat gcgtccgaaa ctctaccaca tgggcatccg
      361 cagcaccgtt tcgcgtaaca acctttccaa cgccaacgag caacgcgact ggcgcatgta
      421 cgcagagttc gctcaacacc tgatcagcaa agctcgtgag ctttacgcgg gtgaagaact
      481 ttccgtcgag ttggaggcat cagtttacgc cttggattcg acgaccatcg acttatgcct
      541 ttcgatgttt ccttgggcgc gttttcgacg gcataaaggc gcaatcaaga tgcacaccct
      601 gatgaatctt cggggcaaca tccccgaatt tattcatatt tccgacggga aactgcacga
      661 cgtcaacatt ctcgactatc ttatccccac gcctggtgca tactatctta tggaccgtgc
      721 ctatcttgat ttcaaccggc ttttcgaaat caacgtctgc cgtgcattct ttgtcaccag
      781 ggccaaatcc aacttcaagt tccaacgtcg atactctcac ccggtcgaca ggaatactgg
      841 cctgatctgc gatcagaccg tgcttctcac aaacttctac ccagccaagg cgtatccaga
      901 acctctgcgc cggatcaaat ttcgcgaccc ggacgatccc agttctctgc tcgtgtttct
      961 ctccaacgat tttacactgc cagccatgac catagccaag ttgtacaaag cacgatggat
     1021 gattgagctg ttcttcaagt ggatcaaaca acacctccgt attaaagctt tccttggcac
     1081 tacgccaaat gcggtgaaga ctcagatctg gatcgccatt tcgacatatt tgctcgtggc
     1141 gatcctcaaa aaacgtctcc gaattgagat ggaactctac acaattctac agatattgag
     1201 cgtctcgctg tttgagaaaa cccccatttt acaggcattt caggcatggg attacaaaac
     1261 caaaacaccc gagagccata accaattact gctgtttggg taatattggg acagcagtga
     1321 tatattttct ttcgtcatgg cgcgcctcct tcaccacaat gcctagcgta agcagttggc
     1381 ggttggcggt caactcacaa acgcaccacc gcattttttc agcaaggcac cacaaaaaga
     1441 ggcacgtcca gttcatcggc ggaaaggccg ccgtgattgc ccggcatgag ttttttgtcc
     1501 ttggcctgcg ccggcggata gagcagggcg tggccgggga cggcaaagag tgtgtaatcg
     1561 ccgagccggt tgagcaaagc ggggtggggg cgcccggggc ccaaaatacc gctgcgcaac
     1621 agggtagcct gaggtacgca cgcgacggcg ccatgcaacg gcgcctggcg ggtcaggcgc
     1681 aggaagtctt ggacccggtt gggccgcaca aaacactgca ccatgcgggc atcgcccgaa
     1741 ggcagaatgg cttgggtgtc atagaggccg gggatttgcg ccagattgat atgcgtggtg
     1801 gtctgcatgt ggccgtggtc ggcggtgacc agcaacagag tatctgttcc agcgagtgcc
     1861 ctttgcaggc ggccaagaaa gacatccaat tcccgaaggt gtttggccgg caatttgccc
     1921 atgggaccat gttcgtggca gagagagtcg tagtgcggcc aataggcata ggccacggcc
     1981 cggccgcgcg aacaggcaaa agcgcgcagc ttctggatga agccggtgag ggtgttgaag
     2041 gagtggcgtt cggtccacca acgctgggcc agcgaggtac ggctggttgg gatataaccc
     2101 tgcgagatga gtacacggcg gcccgggatg gtggccagcg gggaggggag ggccaaatag
     2161 tcggcgatgt tgaactcctc attggcaata ggggtatcca gccgggtgac aaacggcaga
     2221 attgtgccca ccatgcccag atcgggcagg tgcaaatgcc agccgagaat ggcgtgttcc
     2281 gttggtgagg cgccagtagc cagcgtggtg actgcggcgg ccgtggtggc agggcaaatg
     2341 gttgtgattt tttgccatgg gtggcgggcc agaaagtgct gaccgttgcc ggcaaggata
     2401 aagcgatgca attggttggc cccaaggcca tccagcaaca acaccacgac tttgcgcggg
     2461 ttgcggagcg cagcgggggg gagaccggcc aacgcccggt ggggggtgcg cccgccgcgc
     2521 ccgcgaatga gggaggccat tagattaagg agagagcccc cagcatagtt cgggcggggc
     2581 aaatcggtaa aacgcaacgg tttggtcatg gcgaaacggt agctcaaaga tcgggtcaaa
     2641 aaaagtctca aaaaaggctt gctcgggtgt agcgttatag tataacgatt tacataagct
     2701 gatggtcggt gatcaaaact tcacacggga gcgcgcatga aattcagaac gtcaggaatt
     2761 gggctggttg gtttgttgac cttgttgctt ggaatgagcg tgacgtcatg ggccgcagaa
     2821 caagaaggca ttggcagttg gggcgtgaac gatcccacct atggactcaa gacgcgggag
     2881 tttgagccgg gatttacata cactcccagc ccagccgatt ggcgcgacat caatatctat
     2941 cagttgttta ccgaccgctt cgcggattcc ggcgtggacc agttggcgag ctacaagccc
     3001 ggctggaaaa cggaaggcaa gaatttcccc tacaatcgca actatcacca tggtggcgat
     3061 tggaagggct tgaaaaacaa tatcggttat ctgacaggca tgggcattaa ggcagtctgg
     3121 atgtccggtg tgcagatgaa tgaccaaggg cgcgatacca actatacgcc gtatcaccaa
     3181 taccaccccg aaaacttttt caagtgcgac ccagccatgg gcacatttgc tgaattgaag
     3241 gaactgattg acgcgctgca tgccgcaggc atctatgtga ttctggatgt cgcgcccaac
     3301 cacatgtgcg acaaaaacgg cctctggggc aacaaccagc aggatgacaa gcaatactgg
     3361 ggcggcggca ataatacatt cggctggtgg gaccagaaca acaagcatcc ggcgcccttc
     3421 gacaacctca actacttcca caacaacggc accatcaact gctgggactg cagcccggaa
     3481 aacctgctgg gccagttcaa gggcacggac gacctgaaga ccgaagaccc ggcggtgcaa
     3541 agcatacttg ccaaggcgtt caagaacctg attgacgcga cggactgcga cggcttccgt
     3601 gtggatgcca tcaagcacgt ggaatacaac tggatcaagc agtgggctga tgacatccgc
     3661 aagcacgcgg cggctcgcgg caaaaacgat ttcatccttt ttggcgagtt gttttcctat
     3721 gacaacaacg cgctggcgtc tttctgcaag gacttgaatt attcgttcaa ctcggcgctc
     3781 ttcttcccgt tgagccagaa catcaagagc gtgttcggtg atgggcagaa tagcgatcag
     3841 ttgagcaacc agcttaacaa caagtatatg tacggtgaag gcgcggaccg tttggtggcg
     3901 ttcattgaca accacgactt gaaccgcatc gcgctgcaaa tcagtgggca ggatgtgggc
     3961 aacgccgtgt ggaaactcaa acccgcattg agcttcctgt atctcgccac gccagtaccc
     4021 tgcctgtatt acggtacgga gcacgcgttc aaccaaggcg ggcacttcaa cggctccaac
     4081 cgcaccgagg acaatccgga cgacggcgac tggcagcgcg aatgcatgtt cgacaagggc
     4141 ttccagcccg gtcccgcgca gggcaacaag ctggcggcga ccaacgcgcc gctctatcag
     4201 cacattgctg cgctcaatgc ggctcgcgcc acctacaaga gtctgacccg tggctcacac
     4261 cacaccctgg tgaccggcga tgcctatgtg ttccgccgtc tataccaagg cgaggattcg
     4321 ctggtgggca tcaacatcaa cgacggcaac aagagcttcg aggtggaagt gataaaaccc
     4381 caaggcacgg agtacgtcaa cgtcttcaac ccagccgaga aactcattgt tgaccaatgg
     4441 aagaaagtga atttttccct cggtggaaag gaaaccaaga tattcgtgtc ggtctcgagt
     4501 gagtcgccac ctctttgggt tgagaatgtt agccactggc cggtatcggg cgaaatcacg
     4561 tccgacgatg aggtatgggt gaacgcggaa gcgggcctac aggcctcggt ctcgaacgtt
     4621 acggtgcatt acagcgtgaa tgacggggcg tggcagacca aaaccatggc tgtgaacggt
     4681 gactggtcaa gtcagatggg cagttggttc catgtggcgt tgggcccctt cccggcaagc
     4741 gccaacgtga agtacaaagt ggtgctcttg ggcaaaaact ccacatccgt caccaacgac
     4801 aacaatggcg cgaactacag cttcagcgtt gcgcagggca gcgccagcgg cctgtgggtg
     4861 cgcaacgcaa agaactatcc agaggacggc gcggcgacat ttgccactga aatctggatt
     4921 gacgcggaag tcggcccagc cacgggcgtg gacggcgtgt gggttatcta caaggtggat
     4981 aacggcaact ggaccacgaa tgtcatggca gtcaacccgg attggggcag cgaaggcggc
     5041 aagtggtaca acaagaacat cggcactttc aacgaaggtg aagtcgttcg ttatttcgtg
     5101 cgcgcgacag ccggcgcgga aaccgtcgaa gccaataacg ggggcacttt ctaccaactg
     5161 accatccgcg gcgcggggct cgccatcacg aatccgccgg gcaacattgc cgtggcaaat
     5221 aacgtcgcga cctacacgct gcagggcaca gccagcgccg tcaccaacgg caatattttc
     5281 tggaccaact ctctgacggg tgcctccgga cagtttgcgg cggcgacgcc ttggacactg
     5341 gccaacgtgg tgcttggcgt cggggccaac actatcacgg tgtcgggcaa cacgggcagc
     5401 ggcagcggca ccattctgga ggataaaggt tccaactatg gaggcaactg gaccgatggt
     5461 tccaaccaag gcgccgggtt cggcgcgtgg acgttcaatc acttccagga tcctcccaat
     5521 tcctacgccg gcactttcat cggcgatccg gccaaggccg gcatcagcgg ctttggcacc
     5581 aatgccttcg gattctacgc caatccagcg gggtccggtg caaatgcgga agtttctcgt
     5641 acctttgcca acccaatggc cctcggctcg gtgttcagct tcaagtgggg cctgaactgg
     5701 gacagcaacg cggagaattc gtaccgcggc ttctcgctgt cggcgggtga cactgaactg
     5761 atttatatca accagggcaa cacggctgct atcaacatca acggccagca gatgtttagc
     5821 gaatacggca cgcaagccat ggtgttgagc tttgagtatg ttgcgtccgg cagcatccgc
     5881 gtgacggcca atggccgcaa tggaagcgaa aactacaacg agcttctgag cgtgcccgca
     5941 ggtgcgccat cgaagatcaa gttttactac aacgcgtcgg atcgaacacc ggaaccggag
     6001 gatgaagata agcgtcagat gtatgtggac gagttgaaga tcaccggagc gggcggcggc
     6061 gaaccgctga gtgcggtggt gacgatcacg cgtcaggacg tcccagtgga cagcaacggt
     6121 gatggggtgc cggatgcttg gctggcggga cacggactcc ccaccagtac agacgggggc
     6181 agtgtggcgc ctaacggcta cacctactnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnngt gcgtttctat gatgcagtgg cagaaaacga gcatgtgacc gtgagcgcga
     6361 ctccgggtgc gggtagcttc acaaatccgg cgggcattac cgtcacgctg catgtcagcg
     6421 gagtgaatgt catcacctcg acgtacgcgg tggccggcgg cgcgcccgcg gggttcacca
     6481 acggccagca aatcacgatc ggcaccggct ggacgaacgg ccagagcgca ctgctgaccc
     6541 tttatggccg taccaagacc gggatcacgt ctgacaagca gtatcgctac acctacgttg
     6601 agccaagcgg cgtggcgatg acccatgtgg gcggcacgca ccactgggtc agcgacgaag
     6661 gacaggtgtt tatcaactcc gccggatatc cggaaggggc gtcaacggag gcctatgtag
     6721 tgtactgcat caatccgccc tgtgagggtg cctggcctct gattccgatg gagcgcatcc
     6781 cggactggga aaacggcgac tggtggcaca aggatctggg catccagccc aacggcacag
     6841 aaatcgcatt tgccatcatg atgaaggatt cgtttggaac cgaatactgg gacaacaatg
     6901 gcacatcgaa ctatgtggtg actatccagt ccggcggcgg cggcagtggc ggcggcgatc
     6961 caccgccgca gggcggcgac aagccgtatt ccaccaaccc caccaagggc aagcgcggca
     7021 cgatgaccat tgacggttcg cccagcggct ggacggatga acatctgatt gcgctggaca
     7081 tggcgaatga tgatccgcgc tcgctcggcg acaactggac aatgcatgag gcgccgattg
     7141 accttaccca catgtgggcg gcgtgggatg acaacaacct ctacattgcg tggcaatatg
     7201 tggacgtgac ggacatcatt gatccggcca acgcaggcag cgccggcggc ggcaagattt
     7261 ccaacaacga cggtattctg cagtggatcg tactggacac catagccagc cagggcgcca
     7321 cctccgacat gtgggccaag aagagtagtt ggaccggtcc caacaagccg gattaccaaa
     7381 tctatctggc aggcagcctg tggcagggct acatctcacg cgcggtgggc ggcgtgttcc
     7441 ccgtggatga tggcggggtg aactacaaca ccatcgccgc ggcgggcatt actgtggcca
     7501 agggcagtac cttcggcggc accagcctct ggggcgtggg cgacgcggat cacaggaaga
     7561 acggcgacgc ccccaaccgt aacttcctcg cggaagggca cagcaccgcc cgcgacagct
     7621 tctacgagat gagcattccg ctgagctatc tcaacatcac ggcggcccag ctcgattccg
     7681 gtgcaggcgt ggggatcatg attggcggcg gttccacctc cagcatggac tccatcccgc
     7741 acgatgaaac cacgttggac acccccggcg tcgaaagtta caactcgtcg ttcgagtggg
     7801 gcgatagcga tatcttcacc acgccttttg cgcgtatcgg tcactaattg attgtggcgg
     7861 gcgggttgtt cagccaactc gcccgcccac aagtccatgc gtccgcgtat gcttggcgat
     7921 ggtccggctg cgtggcttgc cgatggcggg gattgcgtgg cgcttattta tcgcgcttat
     7981 aaaccggatt gccttcggca tctacgccat aaatggggaa gccgcgatca tcaaagccaa
     8041 tcaagtgttc tttggtggcg cggtcatgga gccggcggct ttcgtccaag actgcgcgcc
     8101 cgggatcgtt ggggtcctgc gtcgtctgat ggccgcaacc gcaaccgctg gcgaagccca
     8161 gtccacataa taataggact gtccgcagca tccacttcat gcaggcattc taacaaatcc
     8221 cgcgtgtcgg cacacgggaa aaacacgacg atttgctttt gccgccaaaa ggggtagctt
     8281 ggtttttccg caagtagatc gtgtacggaa gaggatggat ggccatggat agtcaaaacg
     8341 cacaacaggg aaactccgaa aatccggcgc cggaaaaaat ggtggtggtg gtggatgagc
     8401 cgctgatgcc gcctttttgg ggcgcgcagg tgtgggagcc aacgattgaa gaggtctatc
     8461 cccacattga acggctctcg ctactggtag ggcgctgggg ttttaaaaaa ggcaacctcc
     8521 cggaagcgga ataccggcaa atcctcgaag gcgaagcgca aagccatttt gagcggttga
     8581 agcgggaaaa tcttgagcgc cggcttttgg tgccgcgtgc ggccgtggca tggcacacct
     8641 gccggccgga aggcgactcg ctgatcatga ccgcgcacgg gaccggcaaa acgtatgtgc
     8701 agaattttcc ccggcagcag atcaacgaga agctgtgctt atccgacttc taccgcaagg
     8761 agggggatat tctgggctgc ttcaccgtga cagtgggcag tgcggaggcc accgctgaag
     8821 tgttgcggat gaaagaggat ggggaatacc agaattactt cttctggatg ggctttgcgg
     8881 cagaatatgc cgatgcggtg gcggaatggg cgcaccggaa aatgcttgag ccgctgggtg
     8941 gacgcaaagg ggcgcgcttt ggcccgggct atccgtcctg cccggacacc ggtttgagcc
     9001 gcaccattgc cgaattgacc gaagcctggc gcatcggcgt gacggtgacc gattccaaca
     9061 tgcttgaacc tgaaatgagc accagtgccg tgatggccca ccggccgcgc gcgcgaatct
     9121 tcaaccgcct tgtataacgc gctgcccgcg agggcgtggc aagcgtt
//
LOCUS       DAOPNP010000100         9125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8548_length_9125_cov_9.325469, whole genome shotgun sequence.
ACCESSION   DAOPNP010000100 DAOPNP010000000
VERSION     DAOPNP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8548_length_9125_cov_9.325469"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..210)
                     /locus_tag="PKI64_08740"
     CDS             complement(49..210)
                     /locus_tag="PKI64_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE01130.1"
                     /translation="MSKHQHLTHEDRIEIEVSLRQGHSIRLIAARLGKSRSCISREIR
                     TRSVHSEKA"
     gene            949..>2611
                     /locus_tag="PKI64_08745"
     CDS             949..>2611
                     /locus_tag="PKI64_08745"
                     /inference="COORDINATES: protein motif:HMM:NF026052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE01131.1"
                     /translation="MPGDWGKNAIAYNLLVRYGAAFDHNGDGRIACDPLASGWRETGT
                     FLKAITMLPYIRSLGCNTIHLLPITAIGQDGNKGDMGSAFAIRDPYALDEHLTEPALG
                     LGVETEFAAFVEAAHHLGLRIVVEFVFRTAAKDVAWAKEHPEWLYWIRADVPDRQPGE
                     KSENTYGAPLFTPDELRQIYDAVGNGRFGELLPPHQIYRDMFTAPPHPDTVRMENGAW
                     RGTAPDPRTGEMVTVRIPGAFCDWGPDSEQPPWTDVTYLRLYDHPDFNYIAYNTVRMY
                     AAQLARPENIVRPLWDKITEVIPHYQTHYGVDGVMIDMGHALPAELKRGLVARARQVD
                     PDFALWAEEFNLTPHAREEGYNVCVGPFMQTVRDTERFRGWLRWIHGAGLPVPFMAMA
                     ENHNCPRAVHWPGGRAYALYAQAMGAFLPGVPYLHNGIELAEPTPVNTGFDFTNEEVA
                     QLPPAVLPLFSAAAMDWTRAENLIEPLRRVHAVRAKYAELLADATPGTVIPLETANRA
                     ILGYLRQQPGGKKALAVLGNSDWHQPQPLTLPHPNAKRDLLTNGPL"
     assembly_gap    2612..2711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2874..4508
                     /locus_tag="PKI64_08750"
     CDS             2874..4508
                     /locus_tag="PKI64_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HOE01132.1"
                     /translation="MSKTFDHLRQSYQARVQAAGAFTQNKFVAKENAFALLNAVLRSG
                     DRVCIEGDNQKQADFFARELTKLDPGRINRLHMIQSALVLDAHLDVFRRGIADRLDFA
                     YSGPQSRALYQLAAEKKVSIGAIHTYLELFGRYFVDLYPQVAILVAEACDEAGNIYTG
                     SNTEDSPLIAEATRFKMGVVIVQAKQKLVQLPRVDIPASWVDFIVVTGEDYHLQPLFT
                     RNPAKITNQQVLMAMMALKGIYQPYGVQALNHGLGYATAAIELLLPTYGAQLGLKGKV
                     ATRWVLNPHPTLIPAIEAGFVEHVYAFGGEPGMEDYVRARPDIFAVGPDGTMRSNRCY
                     AHIAGLYAIDLFIGATLQIDRWGNSSTAIKGMIAGFGGAPNLGGTPPGRRHNSPATTT
                     AGCMREQAFRGRKLVVQMTPTRSEKKNIPVFVEELDAQQLYKDGVFAEPPVMIAGDQV
                     THIVTEVGIAYLDKCIDSATRQQAVAAVAGDTPVGQTSTPAQRDALRAAGLVKTVADL
                     GLDPAAATTDLLPVHSLDDLVAASGGLYRIPTGCTG"
     gene            4538..5431
                     /locus_tag="PKI64_08755"
     CDS             4538..5431
                     /locus_tag="PKI64_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOE01133.1"
                     /translation="MKKGKKASPATTRRVVVAAAQFAVVPNDIAANVERIIHYLRRAA
                     REVNAKLLVLPESITTGYNPAMPPAELYDFLPRTAALLAPIQAACRELRIHCVVPTYE
                     RGPKRGVVYNSAFLIDSRGENLGAYRKTHLFPTERLENNGWATPGRDFPVFRTEIGTI
                     GIHICYDGDFPEISRILAVKGARILCRPSALMRSFEIWEGTNQMRAYENHLYHIAANA
                     VGADAAGTTYFGHSMIVSPLAQTVALARAVDDLIYAELDPHPRRRVSFGSDMPMLFDH
                     LADRNVRSYTRDLFRPSQTPS"
     gene            5586..6779
                     /locus_tag="PKI64_08760"
     CDS             5586..6779
                     /locus_tag="PKI64_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01134.1"
                     /translation="MMPSATKRLSTLLTSVALASLLHLPALAQQSQQGPDDSPEALYQ
                     SGMSRFVAGNYEQSVEFLEQLVKVFGKEPELRTQIDLAMYARACALYNLSRWADSIKA
                     FEEYIAQYPESKFADEAMFRIASAQQQLEEYELAVAAYQKLRSAYPRSPYSEDALYQT
                     GICRLIQENHALAAAAFADFMRLYENSPLWGQAGAFCARATFDDGKGKEAIALLETIE
                     KRPRSWSVITYCNFLAFEIGDYMFNDTEYELALKAYRRVKTRRALQTHMQNYVRTLEA
                     QLAATQSGRMDMRNLRSHFQEERRLANELAQAKEMATKLDELPDYDANLFHRIGRCFF
                     NTDRYWEARAAFTRVVAIATEDTVREAGHFDLILAISRLRRFNDLVIECDHYLSTYDP
                     EGKWE"
     gene            6776..>7338
                     /locus_tag="PKI64_08765"
     CDS             6776..>7338
                     /locus_tag="PKI64_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOE01135.1"
                     /translation="MNTMRPPRLTLSALAGGLLAGLLLGALPAHAAAGKWEKLEDCVL
                     ADGYMDGDSFHIRHKNRDLIIRLYYVDCPETNKEFEDRNKDQAGYFNISDTQVVPLGN
                     LGKKFTRKTLAGQKFVVWTRWEDAMGSSQQKRFFGLVQVGKDDLGELLVANGLARVYG
                     ASTVLPNGNSVDSQFERLRHLERRAKA"
     assembly_gap    7339..7438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7439..9004
                     /locus_tag="PKI64_08770"
     CDS             <7439..9004
                     /locus_tag="PKI64_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01136.1"
                     /translation="DAEIEMRKLVRRFPEHPQRARIDFYLALSIAMQERFDEAIKRFQ
                     AWLQAYPTHNLAPDVQYWLPISYYYNGQYAEALPLFLDYAKNNPMNNYAPEALYRAAC
                     CRYALEEYQQCADDVAAWLVQTPNHYFRHEAAIMRGDALAALGELDAAKESYHQAMVP
                     DAGPFYYMALTQIARVYKALAETNEYVAMSRDYVQFIKDMPNDGNIIDAAYNAGWALK
                     QIKQPDKARDLYWQMIERHGNTPAWEGFDLMLSDLAGMYPRGSDAYANELRTRYDKAL
                     AAQRLTLAARLAWAQAQTLPADQQARQLAIFSGRFKPEYLGAETLIWLGRSWIENGMP
                     DSGIPNLEMLLEKYPDSRHVPAAQSLLAQQALQHKDYTTALLYSDSVLNNAAEVDILL
                     ESTFTRAEALLGLQRYGEAISDFNTILANRAAPRRLKPDALLGIAACMAAQQEYRQAI
                     AYYQRIYVLYGAYKKAVIEAYLRSGDCFEKLGDKTAALNTYQEFLNSDYAPGTAAAAF
                     ARQRIAQLAGGAP"
BASE COUNT         1723 a         2984 c         2626 g         1592 t
ORIGIN      
        1 ttttggcgtg aataccttac ctttcttctg ggtgtcccat tgttgaactc aggccttctc
       61 gctgtgcacg gagcgggttc tgatttccct tgagatgcag gagcgggatt tgccgaggcg
      121 ggcggcaatc agccgtatgg aatgtccctg ccgtagagag acttcaattt cgatccgatc
      181 ctcatgcgtg aggtgttgat gtttgctcat tgggttctcc ttatgttgct gggttgcccc
      241 agggttggtc aggagaatga cccaaaaact tggcagacta aatgcccccc cctcccccgg
      301 gggagggggg ggaagaggag ttgcggattc cctccctcat caacccctga gggtttggca
      361 gactaaatgc acccccgcca tttggtagac taaacgcacc cccaagagac cctggggggt
      421 gcatttactt tgtcaattga cccccttatt ttttcctaat atttagctga ttaaaacgct
      481 tgatttcact tataattata agtgaaataa tcttaaactt ccgaaggagt ttttcccctg
      541 ggcaatgaga tatgggtggt gctgcttgta aagcgcgggt cgcggaagtt tcgagggcag
      601 aaatggcgtt atgggctgag ttgccgccga tggccggggg aatgttttgg gcagaggggg
      661 tgtggagcac tcgattggcg ggcgatcaag taaagcgttt ttagttgcaa tataaagcgt
      721 ttcgattatg gtggggtctt ggttgtgaag aaaagagcct tacagcgagt catttcccgt
      781 ttggaagagc gggcacaggc gccgtcgccg gcctatctgg tgcccggtct ctggctggac
      841 ccgcatggtt cattgtcaac gcaatcagtc gttccggaag agtatttcct gcaaagcctg
      901 cgcaggattg tgaggagtcc gaggcgcaag cgggtgacgg atggcgatat gccgggcgat
      961 tggggcaaaa atgccattgc atataacttg ctggtgcgct acggcgcggc gtttgatcac
     1021 aacggcgatg gacgcattgc ctgcgatccg ctggcgtccg gctggcgcga gacgggcacg
     1081 tttctcaagg cgatcacgat gttgccctat atccgctcgc tcgggtgcaa caccattcac
     1141 ctgctaccga ttacggccat tgggcaggac ggcaacaagg gggacatggg cagcgcgttt
     1201 gccatccgcg acccctacgc gttggatgag catctgaccg agccggcgct ggggctgggg
     1261 gtggagaccg agtttgcggc gtttgtcgag gcggcgcatc atctggggct gcggatcgtg
     1321 gtggagttcg tatttcgcac ggcagccaaa gatgtagcgt gggccaagga gcacccggag
     1381 tggctgtact ggatccgcgc ggatgtgccc gaccggcagc cgggcgagaa atccgagaac
     1441 acctatggcg cgccgctgtt cacgccggac gagttgcggc agatttatga cgcggtgggc
     1501 aatgggcgct ttggcgagtt gctgccgccg caccagattt atcgcgacat gttcacagcc
     1561 ccgccccacc cggacacggt gcggatggaa aacggcgcgt ggcgcggcac ggcgcccgac
     1621 ccgcgcaccg gcgagatggt gaccgtccgc attcccggcg cgttttgcga ttgggggccg
     1681 gattccgagc agccgccgtg gacggatgtg acgtatctgc ggctctatga ccacccggat
     1741 ttcaactata ttgcctacaa cacggtacgg atgtatgccg cgcagttggc gcgtccggaa
     1801 aacatcgtgc ggccgctgtg ggacaaaatc acggaagtca tcccacacta ccaaacgcac
     1861 tacggggtgg atggcgtgat gattgacatg ggtcacgcgc tgccggccga actcaagcgc
     1921 ggcctcgtgg cgcgggcgcg tcaggtggac ccggacttcg cgctatgggc ggaagaattc
     1981 aacctgacgc cgcacgcccg cgaagagggg tacaacgtct gcgtgggccc cttcatgcaa
     2041 accgtgcgcg acaccgagcg cttccgcggc tggctgcggt ggattcacgg cgcggggtta
     2101 ccggtaccgt tcatggcgat ggcggaaaat cacaactgcc cgcgcgcggt gcattggccc
     2161 ggcgggcgcg cctatgcgct ttatgcccaa gccatgggcg cgttcctgcc cggcgtgccg
     2221 tatctgcaca acggcatcga gctggcggag ccaacgcccg tcaacactgg ctttgatttc
     2281 accaacgaag aagtggcgca attgccgccg gcggtgctgc cgctgttcag cgcagcggcg
     2341 atggactgga cgcgcgcaga gaacctcatt gagccgttgc gccgcgtgca tgccgtgcgc
     2401 gccaaatatg ccgaacttct ggcagacgcc acgccgggca cggtcattcc gctggaaacg
     2461 gccaaccgcg ccattttggg ctatctgcgc cagcaacccg gcggcaaaaa agcgctggcg
     2521 gtgctgggca acagcgactg gcaccagcca cagccgctca ccctgcccca ccccaacgcc
     2581 aagcgcgacc tgctgaccaa cggcccactg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nctcaccctg ccccacccca acgccaagcg cgacctgctg accaacggcc
     2761 cactgccggc ggttttgcag ccggggcaag tcgtggtgta cgccatcggc tgaacgcgcc
     2821 gcgcttttac cggttgaatt ggcttgccgg ttggcctagc atggccgcca attatgagca
     2881 aaacattcga ccacctccgc cagagttacc aggcccgcgt gcaagccgcc ggcgccttca
     2941 cccaaaacaa gttcgtggcc aaggagaacg cctttgcgct gctgaatgcc gtgctgcgca
     3001 gcggcgaccg cgtctgcatc gagggcgaca accaaaagca ggccgacttc ttcgcccggg
     3061 agctgaccaa gctggacccg gggcggatca accgcctgca catgattcaa tcggcgctgg
     3121 tgctggacgc gcatttggat gtattccgcc gcggtattgc cgaccggctg gacttcgcct
     3181 attccgggcc gcagtcgcgc gcgctctacc aattggccgc tgagaaaaag gtaagcatcg
     3241 gcgccatcca cacctatctg gaattgttcg gccgctactt tgtggatttg tatcctcagg
     3301 tggccattct ggtggcggaa gcgtgtgacg aagcgggcaa catctacacc ggcagcaaca
     3361 cggaggactc gccgctgatt gccgaagcca cgcgcttcaa gatgggcgtg gtgattgtgc
     3421 aagccaagca aaagctggtg caattgccgc gggtggacat cccagcctcg tgggtggact
     3481 tcattgtggt gacgggcgag gactaccact tgcagccgct cttcacgcgc aacccggcca
     3541 agatcacaaa ccagcaggtg ctgatggcaa tgatggcgct gaaaggcatt tatcagccgt
     3601 acggcgtgca ggcgctgaac cacggccttg gctacgccac ggcagccatc gaactgctgc
     3661 tgcccaccta cggcgcgcaa ctgggcctga agggcaaggt ggccacgcgc tgggtgctca
     3721 acccccaccc cacgctgatc ccggccatcg aggccggctt cgtcgagcac gtctatgcct
     3781 tcggcggcga gccgggcatg gaggattacg tgcgcgcgcg gcccgacatt tttgcggtgg
     3841 gccccgacgg caccatgcgc tccaaccgct gctacgcgca catcgccggc ctttacgcca
     3901 ttgatctgtt catcggcgcc acgctgcaga ttgaccgctg gggcaactcc tccaccgcca
     3961 tcaagggcat gattgccggc tttggcggcg cgcccaacct cggcggcacg cccccgggcc
     4021 gtcggcataa ctcgcccgcc accactaccg ccggctgcat gcgcgagcaa gccttccgcg
     4081 gccgcaaact ggtggtgcaa atgaccccca cccgctctga gaagaaaaac atccccgtgt
     4141 ttgtcgagga actcgacgcc caacaacttt ataaagatgg cgtattcgcc gagccgccgg
     4201 tgatgattgc cggcgatcaa gtgacgcaca tcgtcacgga agtcggcatt gcctacctcg
     4261 acaagtgcat tgacagcgcc acgcgccagc aggccgtggc cgcagtcgcc ggcgacaccc
     4321 ccgtgggcca gaccagcaca cccgcccagc gcgacgcgct ccgcgccgcg ggcctcgtca
     4381 agacggtggc tgatctgggg ctggaccccg ccgccgccac caccgacctg ctgccggttc
     4441 attcattgga tgaccttgtg gccgcctccg gtgggttgta tcgtattcct accggctgca
     4501 ccggctgacc agagcccccc gcatgaaagg gaacgccatg aaaaaaggaa agaaagcctc
     4561 gcccgccaca acccgccgcg tggtggtggc cgccgcgcaa tttgcggtgg tgcccaatga
     4621 catcgccgcc aatgtggaac gcatcattca ctatctgcgt cgcgccgcgc gcgaggtcaa
     4681 cgccaagctg ctcgtgctgc cggaatccat caccaccggc tacaacccgg ccatgccgcc
     4741 ggcggagctg tacgactttt tgccgcgcac ggcggcgctg ctggcgccca tccaggccgc
     4801 ctgccgcgag ctgcgcatcc actgcgtggt gcccacctat gagcgcggac ccaagcgcgg
     4861 cgtggtctat aactccgcct tcctgattga ttcgcgcggc gaaaacctag gggcgtaccg
     4921 caagacgcac cttttcccta ccgagcggct ggaaaacaac ggctgggcca cccccgggcg
     4981 cgactttccc gtcttccgca ccgagatcgg gaccatcggc atccacattt gctacgacgg
     5041 cgacttcccg gaaatctccc gcatactggc ggtgaagggc gcgcggattc tgtgccggcc
     5101 ttcggcgctg atgcgcagct tcgagatttg ggaaggcacc aaccagatgc gcgcctatga
     5161 aaaccacctg tatcacatcg cggccaacgc ggtgggcgcg gacgccgccg gcacgaccta
     5221 ttttgggcat tcgatgattg tctcgccgct ggcgcaaacc gtggcgctgg cgcgggcggt
     5281 ggatgacctg atctacgccg agctggaccc ccacccccgc cggcgggtca gttttggctc
     5341 cgacatgccc atgctcttcg accatctggc cgaccgcaac gtgcgcagct acacgcgcga
     5401 cttgttccgg ccgtcgcaga ccccgtcttg agcagaaacg ggcgccccct cctccccggc
     5461 gtgggcgcgg aaaagcggcg cggcgcggcg gatgggccgc gccggttcgc cgtgtaaaac
     5521 gcaaaactta tccgcctaaa aaaaggcgtg atttcccgtt gcgcatcccg tagatttttc
     5581 cacgcatgat gccaagcgcc accaaacggt tgtcaaccct cctgacgtca gtggccctgg
     5641 cgtccctgct gcaccttcct gccctcgccc agcaaagcca gcaggggccg gatgattccc
     5701 ccgaagccct ctaccaaagc ggcatgagcc gtttcgtggc aggcaactat gagcaatccg
     5761 tcgaattcct cgaacagctg gtcaaggtgt tcggcaaaga gcccgagctg cgcacgcaga
     5821 ttgatttggc catgtacgcg cgggcgtgcg cgctctacaa cctgagccgc tgggcggact
     5881 ccatcaaggc attcgaggaa tacatcgcgc aatatcccga atccaaattt gccgacgagg
     5941 ccatgttccg gattgcctcc gcccagcagc aattggagga atacgagctg gccgtggcgg
     6001 cctaccagaa gctgcgctcc gcctatccgc gctcgcccta ctccgaggac gcgctctacc
     6061 aaaccggcat ctgccggctg atccaggaaa accacgcgct ggccgccgcc gcgtttgcgg
     6121 actttatgcg gctctatgaa aacagcccgc tgtggggtca ggccggcgcg ttctgcgcgc
     6181 gcgccacgtt tgatgacggc aaaggcaaag aggccatcgc gctgctggag accatcgaga
     6241 agcgcccgcg ctcgtggtcg gtcatcacct actgcaattt cctggcgttt gaaatcggcg
     6301 actacatgtt taacgacacc gaatatgagc tggcgctgaa agcctaccgc cgggtgaaga
     6361 cccgccgcgc gctgcaaacg cacatgcaaa attatgtgcg cacgctcgaa gcgcaactgg
     6421 ccgccacgca atcgggccgc atggacatgc gcaacctgcg ctcccacttc caggaggagc
     6481 gccgcctcgc caacgaactc gcccaagcca aggaaatggc caccaagctc gacgaactgc
     6541 cggactatga cgccaacctg ttccaccgca tcggccgctg cttcttcaac accgaccgct
     6601 actgggaggc ccgcgccgcc ttcacccgcg tggtggccat tgccacggaa gacaccgtgc
     6661 gcgaggccgg ccacttcgat ttgatcctcg ccatctcgcg cctgcgccgc ttcaacgatt
     6721 tggtcatcga gtgcgaccac tatttgtcca cctatgatcc ggagggcaaa tgggaatgaa
     6781 caccatgcgc cccccccggt tgaccctgag cgcgctggct ggcggcctgc tggccggtct
     6841 gctgctcggc gcgctgcccg cccacgccgc cgccggcaag tgggaaaagc tggaggactg
     6901 cgttcttgcc gatggctaca tggacggcga cagcttccac atccgccaca agaaccgcga
     6961 cctgatcatc cggctctact atgtggactg ccccgaaacc aacaaggaat tcgaggaccg
     7021 caacaaggac caggccggct acttcaacat ctccgacacg caggtggtgc cgctgggcaa
     7081 cctcggcaaa aagttcaccc gcaagactct ggccggccag aagttcgtgg tctggacccg
     7141 gtgggaagac gccatgggca gcagccagca gaagcggttc ttcggcctcg tgcaggtggg
     7201 caaggatgac ttgggcgaat tgctcgtggc caacggcctc gcccgcgttt atggcgcatc
     7261 caccgtactg cccaacggca actccgtgga ctcgcaattc gagcgcctgc gccacttgga
     7321 gcggcgcgcc aaggccaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     7441 tgccgaaatc gagatgcgca agctcgtgcg ccgttttccg gagcatccgc agcgcgcgcg
     7501 gattgatttt tatctggcgc tgtccatcgc catgcaggag cggtttgatg aagccatcaa
     7561 gcgcttccag gcctggctgc aggcctaccc cacgcacaac ctcgcgccgg atgtgcaata
     7621 ctggctgccc atttcctatt attacaacgg ccaatacgcc gaggcgctcc cgctgttcct
     7681 ggactacgcc aagaacaacc ccatgaacaa ctacgccccc gaggccctct accgcgccgc
     7741 ctgctgccgc tacgcgctcg aagaatacca gcagtgcgcc gacgatgtcg ccgcctggct
     7801 ggtgcaaacc cccaaccact acttccgcca cgaagccgcc atcatgcgcg gcgacgccct
     7861 cgccgccctt ggcgaactcg acgccgccaa ggagtcctat caccaagcca tggttcccga
     7921 cgccggcccg ttctactaca tggccctcac ccagattgcc cgcgtctaca aggccctcgc
     7981 cgaaaccaac gaatatgtcg cgatgtcgcg cgactacgtc caattcatca aggacatgcc
     8041 caacgatggc aacatcattg acgccgccta caacgccggc tgggccctca aacaaatcaa
     8101 gcaacccgac aaggcgcgtg acttgtactg gcagatgatc gagcgccacg gcaacacccc
     8161 cgcctgggaa ggctttgacc tgatgctcag cgacctcgcc ggcatgtacc cgcgcggcag
     8221 cgatgcctac gccaacgagc tgcgcacccg ctacgacaag gcccttgccg cccagcgcct
     8281 gaccctcgcc gcgcgccttg cctgggcgca ggcccaaacc ctccccgccg accagcaggc
     8341 ccgccagctc gccattttct ccggccgctt caagcccgaa tatcttggcg cggaaaccct
     8401 catctggctt ggccgttcgt ggatcgaaaa tggcatgccc gattccggca ttcccaacct
     8461 cgaaatgctc ctggaaaaat atcccgactc gcgccacgtc cccgccgcgc aatcgctcct
     8521 ggcccagcag gcgctccagc acaaggacta caccactgcg ctgctctaca gcgactccgt
     8581 cctcaacaac gccgccgaag tggacatcct gctcgaatcc accttcacgc gcgctgaagc
     8641 gctccttggc ctccagcgct acggcgaggc catcagcgac ttcaacacca tcctcgccaa
     8701 tcgcgccgcg ccgcgccgcc tcaagcccga cgcgcttctc ggcattgccg cctgcatggc
     8761 cgcgcagcag gaataccgcc aggccatcgc ctactaccag cgcatctacg tcctctacgg
     8821 cgcctacaaa aaagccgtca tcgaggccta cctgcgcagc ggcgactgct tcgagaaact
     8881 cggcgacaaa accgccgccc tcaacaccta tcaggaattc ctcaacagcg actacgcccc
     8941 cggcaccgct gccgccgcgt tcgcccgcca gcgcatcgcc caactggccg gaggtgcccc
     9001 atgacccgcc tgcagcaact tttccacact gtggaaaaat caccgcaaag tttgccgcag
     9061 cgctcgctac ggctcgcttg ctcgcccggc tgcgccggtc gctatagagc tcgcgttcgc
     9121 ccggc
//
LOCUS       DAOPNP010000101         9050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8639_length_9050_cov_7.474708, whole genome shotgun sequence.
ACCESSION   DAOPNP010000101 DAOPNP010000000
VERSION     DAOPNP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_9050_cov_7.474708"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PKI64_08775"
     CDS             complement(<1..1327)
                     /locus_tag="PKI64_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01137.1"
                     /translation="MKQKWFLSLLIGLLACSVSAFAQDSRDVTFSVNMTVQIEKNNFN
                     PATDTVYVRGELNEWGQTAMSREGITDIWSVTAPVVGAANTTSQYKFCIGDEGWESRG
                     NRTFQLGPANTPQTLDIVFFNDEEPVGDEVQAAVTFSVDMSVEIAENRFDPEGGQDVL
                     VRGSFNGWAGNAAELDREDNTSVYSGTFNITTQENALVLYKFVYTTSGDDAWEGKIDN
                     RSFRMGDGSPQTLPTVVFNNETPVQPKDLQFTAGTWNPAATGNYEFELTRVDDPMDPG
                     QIALSSDNEDAVTVPLSVTFDTGSNTVTFLANVVSLTNGDATITASNTTSGAWDTYTV
                     RAPTLAINGPWEIYAAGPVSYVLTRFAGIGDNIALESSDTDVLTVPASVTFGGDSNEV
                     AFAATAIAVGQATITATAANGIKAEFNVTFGAPRLELNGPVSVTAGDSKT"
     gene            complement(1467..3155)
                     /locus_tag="PKI64_08780"
     CDS             complement(1467..3155)
                     /locus_tag="PKI64_08780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE01138.1"
                     /translation="MKHSLTMLLLGLALASGARAQSEGIFADFVTSHGNFTVWLDMDR
                     APRAVASFVGLATGEGGWVDLQTNVWHRPFYDGSLIHRIVKDVVADPPPTVTNDIAIQ
                     GGGLPLEEPSLREQIHSVYTNTYSGSVVSSNAPGITTNLLNLPVVITNPPAVATNYVW
                     KGETVTTVAPRVVRGQITASVVSSNESSGDWVTSRAPVDFGYTNATAATIVTTGIVQV
                     AVLVTNGGSNALATTHTAHVTNQAAERLQVPIVLTNFVNAGYTMLDNVTNGLLHSNGV
                     IAMANSGPNTDCSQFFITVTNAPYWDGGYSVFGHVTDGMATVRAIAAEPVQAGGSRPI
                     ADIVVSNVTIRRVGAAAEAFDISVHGVPSPESVPMDIAMQGVNVQLRMELASQTKPLL
                     FSDSTSMAPFAAWDHQETFTNLVFYTNATQTLTMLLPVAELGPRHFFHAARIRYPIPL
                     STPETHWRRTFTFRSEQLDLTYSATFPTNVMQQGTGWSKQSTNDPVARTIFAGSFWTR
                     DAYMARLNFIDNSGQEYNYTLGFTPGAVTNRFTGTYGLFISPVRYNISGTFTMD"
     gene            complement(3285..4106)
                     /locus_tag="PKI64_08785"
     CDS             complement(3285..4106)
                     /locus_tag="PKI64_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01139.1"
                     /translation="MRKTTTQIWLAIIATGLIFNAAQAQTYGVQPSPAYHQAQPTYFM
                     PAPSARPAVETDDDYADEEGIYWFWGQKWPGLSLGPKFGTTGIGADLTFGINQYLNLR
                     GGFNYGAFTWKTKLGSTKYDMDVDMVTAPILLDIHPLGGHFHLTAGLYIQPDVAADIK
                     ATPTSNKQIGEHTYPPEVIGTLRGEVEAKNAVAPYLGIGFGNTVGEDQLLTLHLDIGV
                     IFQSYDASLTSDGEGMTAKLDTFREDLKKEESNIQHDLNKLGIFPVVTLGIAWHF"
     gene            complement(4147..5859)
                     /locus_tag="PKI64_08790"
     CDS             complement(4147..5859)
                     /locus_tag="PKI64_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOE01140.1"
                     /translation="MSKEQSQATMRIDITPAMLEQLQQAPAPGGVRLGRHAPTHSGGT
                     GVIRIPAGPAARPQGGEDFQTLLQSIYDAVLITDPHGGIVSVNVRAEQFFAGTRADLC
                     RQNVLDVLCGAERTLLDTILGSLQNNRFVLIQALCQRLDGTMFPAEISVNRLPLGGKM
                     HLSFFIRDITLRKETEERLKTGYNALQNSGSGIAVADVELALAGYANPSMLTLLGLGS
                     VEEAYASNFRQFLKDPALAGDMLAAVTAGETWTGELEMQRKDGAPFYAQASLEPNLNP
                     DGAMTGIVISLLDVTPQRLAQQQLESYAAQLRQKNQEMEADLRLARELQYAFLPAEYP
                     QVTTQATGRAGRLDFAHVYHPSGLVGGDFFDILPVSASRVLVFMADVMGHGARAALVV
                     ATLRGLLEQLSKETHDPGEFMTRLNAAYCRIFSSSGDLMFATACCVCFDLDSGRILHA
                     NAGHPLPLVIEPDGTVRGARVAEEALGPALGLFPDAQYGHSRADLLPGKRVIFYTDGL
                     SEARNAAQEEFETTGMTRTLQQQAGAPLPELLAALVRDVVAFTGETVFEDDVCLLGVG
                     YVQD"
     gene            5965..8223
                     /locus_tag="PKI64_08795"
     CDS             5965..8223
                     /locus_tag="PKI64_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01141.1"
                     /translation="MTTAYSPDALQQTRNQALLADARYLRREVVSSVPPLQVDIDQVA
                     RLSAEDVHLFEALQIDAARLAIEAVASLSKINEVDHMGGGLDMIPPLLLTLALVNQAE
                     REFTIENAHTSVGYFAALATLGYLDRQRVCDTFRRGLDIPGHVAWVPGGTQLNGGRLG
                     VMIPTAVGQALGKRAAYGADAWVLCHTGDAGWIAGHALNGFHAAHIHKAPVTFIMHRN
                     GIQLSGTTDTLTGRDPRPIIEALGVQVLETPSLYDVATLWKTLREARSLAREGVPTMI
                     IPTGKKNVTLTQVGERFGITTELSAFAAKNGVEMDRAIWLPGCLMSYRDLPSMMECLF
                     LVNGLPGGKAHHDGHMKGRDLDALLANPMLQFSKKQQAALERWRALAPISVTTTARPA
                     PGSENLVIPAEALAKAEATLPAAGGKWTSPRAGVATAYELVAKTHPDAMFVIDCDLAP
                     STKVDLALKVLDANHQFQLSIEEQIATMMANGLAMSSPDPKLVVFATFAAFFEGIARE
                     GFEMWRYQRNLTGANEGLNVTFHLSHVGSCTGRDHFSGWSLDWINLAIGYLPYLDRFY
                     APADARAAFVAICDLASRSGGHILGIPRDNLPILTQPDSAAPLWTVDSPWTDVTQLRA
                     APNAKKAILGFGATAFVAAEAAAKLAAENIAVDAWVVNGLPLDAGRLEQFFAQYPDGI
                     VTIEDGLIGTPEQGLRGFAGLVRGAAYGRTIPLQHLGITDPRVAPSEGHLELWQYFGI
                     DVPSAITAVKAL"
     gene            8334..8969
                     /locus_tag="PKI64_08800"
     CDS             8334..8969
                     /locus_tag="PKI64_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01142.1"
                     /translation="MPGENELKPLDISAIKSSTSRIDLSKATIPTSPATSGIRIGANP
                     KKATSRIDINAIPGATKAQTSRVGLGAMPPADDEAFKRRTALLDTSNIPLATTPAPQP
                     RTIRIGSRPTVRVASTATPSTFSPGRSEEAAPAESSGGRPTIKLKRPGGASSITISGG
                     TTAPSVPSEPMFTIEADEGPGAAWSVISLLSMLVVIGLIVYQVLTLNGAKY"
BASE COUNT         1644 a         2811 c         2883 g         1712 t
ORIGIN      
        1 aggtcttgga atcgccggcg gtgaccgaca ccggcccatt cagttcaagg cgcggggcgc
       61 cgaaggtcac attgaattca gccttgatgc cattggccgc ggtggcggtg atggtggctt
      121 ggccgacggc aattgcggtg gcggcgaacg ccacttcgtt gctatcgccg ccaaacgtca
      181 cgctggcagg cacggtgagg acgtccgtat cggagctttc gagggcgatg ttgtcgccga
      241 tgcccgcgaa gcgggtcaac acgtagctga cggggccggc ggcataaatt tcccacgggc
      301 cattgatggc gagggtcggc gcgcgcacgg tgtaggtgtc ccacgcgccg gaggtcgtgt
      361 tgctggccgt gatcgtcgca tcgccattcg tcaggctgac cacgttggcg aggaaggtga
      421 ccgtgttgct gcccgtgtca aacgtgacac tcaaggggac agtaaccgca tcttcgttgt
      481 cgctgctcaa ggcaatttgg cccgggtcca tcgggtcgtc cacgcgggtc agttcgaact
      541 cataattgcc cgtcgcggca gggttccatg tgccggcagt gaattgcaaa tccttgggtt
      601 gaaccggggt ttcgttgttg aacaccacgg tggggagggt ttgcggcgag ccgtcgccca
      661 tccggaaaga gcggttgtcg atcttgcctt cccatgcatc atcgccgctg gtcgtgtaca
      721 caaatttgta tagaacgagg gcgttctctt gggtcgtgat gttgaatgtg ccggagtaaa
      781 cactggtgtt gtcttcacga tccagctcgg ccgcgttgcc tgcccatccg ttgaagctgc
      841 cgcgaaccaa gacgtcctga ccgccttccg gatcaaagcg attttcagca atttccacgc
      901 tcatatccac ggagaaggtg accgccgctt gcacttcatc gcccaccggc tcttcatcgt
      961 tgaagaacac aatatcaagt gtttgagggg tgttcgccgg gcccagttgg aaggtgcggt
     1021 tgccgcggct ttcccatccc tcatcaccga tgcagaactt gtattgtgac gttgtattgg
     1081 cggcacccac taccggggcc gtaacgctcc agatgtcggt gatcccttcg cgggacatgg
     1141 cagtctgccc ccactcattc agttctccgc gcacgtacac ggtatctgta gcaggattga
     1201 aattattctt ctcaatctgc accgtcatgt tcaccgagaa ggtgacatcg cgcgaatcct
     1261 gcgcgaacgc gcttacgctg cacgcaagaa gaccgatcaa cagactcaga aaccattttt
     1321 gtttcatctt accctccgga tggtttggta cccaaaacga ctatcaacca cttcattctg
     1381 ccacagcggg cctgtggcga gcaagcaaaa aacggcaaga aaaaacgctt tatttgcccc
     1441 cccccccccc gcggggcgag cgcgggtcaa tccatcgtga acgtgccgga gatgttgtag
     1501 cgcacggggc taatgaacag gccgtaggtt cccgtgaagc ggttggtgac ggcgccgggg
     1561 gtaaagccga gggtgtagtt gtattcctgc ccggagttgt caatgaagtt gagccgggcc
     1621 atgtaggcat cgcgggtcca gaaactgccg gcaaagatgg tgcgggcgac gggatcgttg
     1681 gtgctctgct tggaccagcc ggtgccctgc tgcatgacgt tggtggggaa tgtggcggag
     1741 taggtcaaat ccaattgttc ggagcggaag gtgaaggtgc gccgccagtg ggtttcgggc
     1801 gtggagagcg ggatcgggta gcggatgcgc gccgcatgga aaaaatggcg cgggcccagc
     1861 tcggcgacgg gcaatagcat ggtgagggtt tgggtggcat tggtataaaa caccaggttc
     1921 gtgaacgtct cctggtgatc ccacgcggcg aagggggcca tgctggtgga gtcgctgaac
     1981 agcaacggtt tggtctggct ggccagttcc atgcggagtt gcacgttcac accttgcatg
     2041 gcgatgtcca tgggcaccga ttcgggactg ggaacgccgt gcacggagat gtcgaaggct
     2101 tccgcggcgg cgccgacgcg gcggatggtg acgttgctga cgacaatatc ggcgatgggc
     2161 cggctgccgc cggcttgcac cggttcggcg gcaatggccc ggacggtggc catgccgtcg
     2221 gtcacgtggc caaagacgga gtagccgcca tcccagtatg gggcgttggt gacggtgatg
     2281 aaaaactgcg agcagtcggt gttggggcca gagttggcca tggcaatcac gccgttggaa
     2341 tgcaacaggc cgttggtgac gttgtcaagc atggtgtagc cggcgttgac gaagttggtg
     2401 agcacaatgg gcacttgcag ccgttcagca gcttgattgg tcacgtgggc cgtgtgggtg
     2461 gtggccagcg cgttgctgcc gccattggtg accagcaccg ccacttgcac aatgccggtg
     2521 gtgacgatgg tggcggcggt ggcgttggta tagccgaaat ccacgggggc gcggctggtg
     2581 acccaatcgc cagagctctc gttgctggag accaccgagg ccgtgatttg tccgcggacc
     2641 acccgcggcg caacagtggt gacggtttcg cctttccaca catagttggt ggcgacggcc
     2701 ggcggattgg taatcaccac cggcaaattc agcaggttgg tggtgatccc cggcgcattg
     2761 gaggatacca cgctgccgga ataggtgttg gtgtaaacgg agtggatctg ctcgcgcagt
     2821 gagggctctt ccaacggcaa cccgccgcct tggatggcga tatcgttggt gactgttggc
     2881 ggcgggtcag ccaccacatc tttgacgatg cggtgaatga gcgagccatc gtagaagggg
     2941 cggtgccaga cgttggtttg caggtccacc cagccgcctt cgcccgtggc caggccaacg
     3001 aagctggcga cggcgcgcgg ggcgcggtcc atatccagcc agaccgtgaa gttgccgtgc
     3061 gaggtgacga aatccgcgaa gattccttcg gactgggcgc gggccccgga ggccagcgcc
     3121 agtcccaaaa gcaacatggt caacgaatgt ttcatggccg agtcgcaatt ccgcgcccac
     3181 tgccggcggg cagtgtgggc ggttcactcc ggatgatagc acctcccgcc ccgcgcggct
     3241 actccatcgc gcggaatcag gctgttttgt ctactgcaaa cgccctaaaa gtgccacgca
     3301 atgccgaggg tcaccaccgg gaaaataccc agcttgttca ggtcgtgctg gatattactt
     3361 tcctctttct tcaagtcttc gcggaaggtg tcgagcttgg cagtcatgcc ctcgccgtcg
     3421 gaagtgagcg acgcatcata ggattggaaa atcacgccga tgtcgaggtg cagggtgagg
     3481 agttgatcct cgccaacagt gttgccgaag ccgatgccca gatacggggc cactgcgttc
     3541 ttggcttcca cctcgccgcg cagggtgccg atgacttccg gcggataggt gtgttcgccg
     3601 atttgcttgt tgctggtggg cgtggccttg atgtccgcgg cgacatcagg ctggatgtag
     3661 agccccgccg tcaggtggaa gtggccgccc agcggatgga tgtccagcag gatcggcgca
     3721 gtcaccatgt ccacatccat gtcatatttg gtggaaccga gcttggtttt ccacgtgaag
     3781 gcgccgtagt tgaagccgcc gcgcaggttc agatattggt tgatgccgaa ggtcaggtcc
     3841 gcgccgatgc cggtggtgcc gaacttgggc cccaacgaca ggccgggcca cttctggccc
     3901 cagaaccaat agataccctc ttcgtcggcg tagtcgtcat cggtttccac ggcggggcgc
     3961 gccgaggggg ccggcatgaa atacgtgggc tgtgcctggt gatatgccgg cgagggttgc
     4021 acgccgtagg tttgggcttg cgccgcgttg aaaatcagtc ctgtcgcgat gatggccagc
     4081 cagatttggg tggtcgtctt cctcatgtag ttgctccttg ggttgctttt aaaagtttgg
     4141 tgggactcaa tcctggacgt agccaacgcc caggaggcac acatcgtctt cgaagacggt
     4201 ttcgccggtg aaggccacga cgtcgcgcac cagcgcggcc agcagttccg gcagcggcgc
     4261 gccggcttgc tgctggaggg tgcgggtcat gccagtggtt tcaaattctt cctgggcggc
     4321 gttgcgggct tccgagaggc cgtccgtata aaagatgacg cgcttgccgg gcagcaggtc
     4381 cgcgcgcgag tggccgtatt gcgcatcggg aaagagaccg agggcggggc cgagggcttc
     4441 ctctgccacg cgcgcgccgc gcacggtgcc gtcaggctca attaccagcg gcagggggtg
     4501 gccggcgttg gcatggagaa tccggccgct gtcgagatcg aagcacacac agcaggcagt
     4561 ggcgaacatc aggtcgcccg agctgctgaa aatccggcaa taggcggcgt taaggcgcgt
     4621 catgaattcg ccgggatcgt gggtttcctt ggaaagctgt tcgagcaggc cgcgcagggt
     4681 ggccaccacg agggcggcgc gcgcgccgtg gcccatgacg tcggccatga acaccagcac
     4741 gcgactggcg gatactggca ggatatcgaa gaaatcgccg ccgaccaagc cgctggggtg
     4801 atacacatgc gcaaaatcca gacgcccggc gcggccggtg gcctgcgtgg tcacctgggg
     4861 atattccgcc ggcaggaacg catattgcag ttcgcgggcg aggcgcaggt cggcttccat
     4921 ctcctggttt ttctggcgca actgggcggc ataggattcc agttgctgct gggcgaggcg
     4981 ctgcggggtc acgtccagca gcgagatcac aatgccggtc atggcgccat cgggattcaa
     5041 gttgggctcc agcgaggcct gcgcatagaa gggggcgcca tccttgcgct gcatttccag
     5101 ttcgccggtc caggtttcgc cagcggtcac ggcggcgagc atgtcgccgg ccaacgcggg
     5161 gtccttcaga aactggcgaa aattgctggc gtaggcttcc tccaccgagc caaggcccag
     5221 cagcgtcaac atggaggggt tcgcgtagcc ggccagcgcc agctccacat cggccacagc
     5281 gataccgctg ccggagtttt gcagcgcgtt atagccggtt ttcaggcgct cttcggtttc
     5341 cttgcgcagc gtgatgtcgc ggatgaaaaa gctcaagtgc atcttgccgc ccaacggcag
     5401 gcggttcacc gaaatctcgg cggggaacat ggtgccatcc agccgctggc acagtgcctg
     5461 gatcagcaca aaacggttgt tctgcagcga gcccaaaatg gtgtccagca gcgtgcgttc
     5521 cgcgccacaa agcacatcca gcacgttttg tcggcagaga tccgcgcgcg tccccgcgaa
     5581 gaattgctcg gcgcgcacat tgacgctgac aatgcccccg tgcggatccg tgatgagcac
     5641 cgcgtcatag atgctttgca gcagcgtttg gaagtcctcg ccgccctgcg ggcgcgcggc
     5701 agggccggcc ggaatgcgga tgaccccggt cccgccggaa tgcgtcggcg catgccggcc
     5761 caaacgcacc ccgcccggcg caggagcctg ctggagctgc tccaacatcg ccggcgtgat
     5821 gtcaatgcgc atcgtcgcct gggattgttc cttggacata tttaagtgaa catagccaac
     5881 cccaccgccc tcggcaattg ttttcctgcc ctaatccgcg gcgggttgac gagccgcgcg
     5941 gcatggggca gaatcccccg cactatgact acagcctatt ctcctgatgc cctccaacaa
     6001 acccgcaacc aagccctgct ggccgatgcc cgctacctgc gccgcgaagt cgtctcctcc
     6061 gtgccgccgc tgcaagtgga tattgaccaa gtcgcgcgcc tgtccgcgga agacgtccac
     6121 ctgtttgaag ccctccagat tgatgccgcg cggctggcca tcgaagcggt ggcctcgctc
     6181 tccaagatca acgaggtgga ccacatgggc ggcgggctgg acatgattcc gcccctgttg
     6241 ctgacgctgg cgctggtgaa ccaggccgag cgcgagttca ccatcgaaaa cgcgcatacc
     6301 tcggtgggct atttcgcggc gctggccacg ctgggctatc tggaccggca acgcgtatgc
     6361 gacacattcc gccgcgggct ggacattccc ggccacgtgg cgtgggtgcc cggcggcacg
     6421 cagctcaacg gcgggcgcct cggcgtcatg atccccaccg ccgtgggcca ggccctcggc
     6481 aagcgcgcgg cctacggcgc cgatgcgtgg gtgctctgcc acactggcga tgccggctgg
     6541 atcgccggcc acgcgctcaa cggtttccac gcggcgcaca tccacaaggc gcccgtcacg
     6601 ttcatcatgc accgcaacgg cattcagttg tccggcacca cggacacgct gaccggccgc
     6661 gacccgcgcc ccatcatcga ggcgctcggc gtgcaagtgc tggaaacgcc ctcgctctat
     6721 gacgtggcca cgctctggaa aaccctgcgc gaagcgcgca gcctcgcccg cgaaggcgtg
     6781 cccaccatga tcatccccac cggcaagaaa aacgtcacgc tgacccaggt gggcgaacgc
     6841 ttcggcatca ccaccgaact gtcggctttt gccgccaaaa acggcgtgga aatggaccgc
     6901 gccatctggc tgcccggttg cctgatgagc taccgcgatt tgccgtccat gatggagtgc
     6961 ctgttcctcg tcaatggcct gcccggcggc aaggcgcatc acgacggcca catgaagggc
     7021 cgcgatctgg atgcgctgct ggccaatccc atgctgcagt tcagcaagaa acagcaagcc
     7081 gcgctggaac gctggcgtgc gctggcgccc atctcggtga ccaccaccgc ccgccccgcc
     7141 cccggcagcg agaacctcgt gattcctgcc gaagccctcg ccaaggcgga agccaccctg
     7201 cccgcggctg gcggcaagtg gacctcgccg cgcgccggcg tggccacggc ttatgagctg
     7261 gtggccaaga cccaccccga cgccatgttt gtgattgatt gcgaccttgc gccctccacc
     7321 aaggtggacc tcgcactcaa ggtgctcgat gccaatcacc agttccagct ctccatcgaa
     7381 gaacaaatcg ccaccatgat ggccaacggc ctcgcgatgt cctcgccgga ccccaagctg
     7441 gtggtgtttg ccacctttgc ggccttcttt gaaggcatcg cgcgcgaggg cttcgagatg
     7501 tggcgctatc agcgcaacct caccggcgcg aacgaaggtt tgaacgtcac cttccacctc
     7561 tcgcacgtgg gttcctgcac tgggcgcgac cacttctccg gctggtcgtt ggactggatc
     7621 aacctcgcca tcggctacct gccctatctc gaccgattct acgcccccgc cgatgcccgt
     7681 gctgcctttg tcgccatttg tgacctggcc tcgcgcagcg gcggccacat tctgggcatt
     7741 ccgcgtgaca acctgcccat cctgacccaa ccggactccg ccgcgccgct ctggaccgtg
     7801 gacagcccgt ggacggatgt gacccaactg cgcgccgccc cgaacgccaa gaaagccatt
     7861 ctgggctttg gcgccacggc atttgtcgcg gcggaagccg ccgccaagct ggccgccgaa
     7921 aacatcgcgg tggacgcgtg ggtggtcaac ggcctgccgc tggatgccgg ccggttggag
     7981 caattttttg cgcaatatcc cgatggcatt gtcaccatcg aggacggcct gattggcacg
     8041 ccggaacagg gcctgcgcgg ctttgcgggt ttggtccgcg gcgccgccta cggccgcacc
     8101 atcccgctgc agcatttggg catcaccgat ccgcgcgtgg cgccgtctga aggccatctg
     8161 gaactgtggc aatatttcgg cattgatgta ccgtccgcca tcaccgccgt taaggcgttg
     8221 tgatgtgcct tgctgccggc cacacggctg ccgccgcgcg cgccggcaat agaggttgac
     8281 gccacacggc acgcgagcct atgatgctcg gcagatagta cggaggaact tccatgcccg
     8341 gcgaaaacga actgaagccc ttggatatca gcgcaatcaa atcatccaca tcgcgcattg
     8401 atttgagcaa agccacgatt cccacatcgc cggccaccag cggcatccgc attggcgcca
     8461 atcccaagaa ggccacctcc cgcattgata tcaacgcgat tccgggcgcg accaaagcgc
     8521 aaacctcccg cgtgggtctc ggcgccatgc cgcccgcgga tgacgaagct ttcaaacgcc
     8581 ggaccgcgct gctggatacc agcaacatcc ccttggccac cacgcccgct ccgcagccgc
     8641 ggacgatccg catcggcagc cgcccgaccg tgcgcgtcgc cagcaccgcc acgccctcaa
     8701 cgttctcgcc cggccgcagc gaagaagccg cgcccgccga aagttccggc ggccgcccca
     8761 ccatcaaact caaacgtccc ggcggcgcca gttccatcac tatttccggt ggcaccacag
     8821 ccccctccgt gccatccgag ccgatgttca ccatcgaagc ggatgaaggc cccggcgcgg
     8881 cgtggtcggt gatttctctc ctctccatgc tggtggtcat tggcctgatc gtctatcagg
     8941 tgctgactct caacggcgcc aagtactaaa cgcgcggctc agtttgccaa cggtcttttt
     9001 gtagggcggg aatggttgag gaacgaaacc ttcccgccgg gaaaggctgg
//
LOCUS       DAOPNP010000102         8963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8750_length_8963_cov_9.500000, whole genome shotgun sequence.
ACCESSION   DAOPNP010000102 DAOPNP010000000
VERSION     DAOPNP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_8963_cov_9.500000"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKI64_08805"
     CDS             <1..304
                     /locus_tag="PKI64_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01143.1"
                     /translation="DLTAPIEGLEWSQTFQPFTAGRFHSSTIPALQKPDKNRHGAMRN
                     SGFPFLLFCISRFPTWFFRSRRRYNGGLHQIRTSYLQARVAPPGGRLFRAMNCPVY"
     gene            complement(483..1205)
                     /locus_tag="PKI64_08810"
     CDS             complement(483..1205)
                     /locus_tag="PKI64_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01144.1"
                     /translation="MKRLVILGILGACATTLAAPAPVRPFEDYQVILDRKPFGAPPDP
                     SQIPERTVPVSESFAASLQLSGIYELADGNLRVAITDKKDNSYFSLMVGEKDRSTGIM
                     LEDADYDAESATLQKDGQVVVLSMNDTAGGQVLSASAGREAIRQAAELRTSYAERRRQ
                     RMLERQKPVEVPKPIYTGEELERHLQEYQMEILRGGNLPPLPVQLTPENDAKLVAEGV
                     LPPVDDEGYEIEYEEDDGYYSY"
     gene            complement(1249..2520)
                     /locus_tag="PKI64_08815"
     CDS             complement(1249..2520)
                     /locus_tag="PKI64_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01145.1"
                     /translation="MNPSTAPATSFWYIPVASALHDARSLSALLAGHEQALVGAGGHR
                     GVGEVADAPGVPFYFVQTGGTEHDVLRRHRSRIQQGRGGPLLLIAHPGHNSLPAALEM
                     LAAIQQEGRPGRIFLLKGKEDDRTLAEITQTARALAANNRLAQDRFGIIGPSSDWLVA
                     SSPRAELVARRFGMWVKEFAVDELRAYIAQQPLPEDGPEFDLWDRAHGKDGLTREAFA
                     QAVRVTRGLRALVQNHQLSMLTLRCFDLVEKDGVTGCLALALLADAGITAGCEGDIPS
                     AVLLRWLWHLTGQAGWMANPSDVDVPKGELLLAHCTVPLGMVGGYRFRTHFESGIGLG
                     IAGEFASGPVTLLRLGGSNLDRWWGAEGTLVENRRAPDLCRTQVVVRVSPPAAGELLS
                     APLGNHLVLVRGHVQGLFREAFIWSTPGRGA"
     gene            complement(2517..3260)
                     /locus_tag="PKI64_08820"
     CDS             complement(2517..3260)
                     /locus_tag="PKI64_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01146.1"
                     /translation="MRMMLILVLAPAAGARAETVLEKVLAPYDSVQSMSCEIRRDTPL
                     PDGNTLRMLSRVYYQRPDKLHVENFSPVKRRTISDGTVFRQYTEGAPKGFSRPVAELD
                     GEMLRNLRMVPGSAANMLEVLRGATEVPLEPTAEFPVRAGYDNQKSFAVLNLDAQGRL
                     ARFELYGSPAMTDLLTQTDFSAFQEVAPGVWIACLQQAKVTVQGMERTETTRVDSPVA
                     NGEIPAAFFKADAFFPAVEFVDSFDKIAP"
     gene            3358..4104
                     /locus_tag="PKI64_08825"
     CDS             3358..4104
                     /locus_tag="PKI64_08825"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOE01147.1"
                     /translation="MPLPRTILLLLPLLLTGGAARAMEPERLEYDITWVGISIGTMVV
                     QNELDADGHPVRLLRMWNRPWIARFYPVDTTIECSIIPDPDGSCHVVHKHVRENTFRQ
                     DDTLQLWPAQGRAIWSNQLTQTATEFTTPTGAHDVVTFFFDLRNMLSNAPGPAGGDYE
                     LVMDDGVHGLTIHMGEAQRIRTPFGRVEAVQVKAISKSPTLFSRNRPKAVWVATSQPV
                     VLFADVDSRFGAVRATLTQWTRGNKPVQAE"
     gene            complement(4116..5177)
                     /gene="tsaD"
                     /locus_tag="PKI64_08830"
     CDS             complement(4116..5177)
                     /gene="tsaD"
                     /locus_tag="PKI64_08830"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOE01148.1"
                     /translation="MLILGIESSCDETAAAVVRDGREVLSSVVHSQIASHQPWGGVVP
                     EIASREHVSKFPEVVVAAMTQAGVAWKELTAIAVTHGPGLVSSLLIGLSGARALGQTL
                     DKPVWGVNHLEAHVAGMFLDSAAPPPATACPALVLMVSGGHTCLILMPGVGRYQLLGQ
                     TLDDAAGECLDKGASLLGLGYPGGPAIEKAARGGDAGFVRFPRGLEHAKQRTTDDGLA
                     IELCFSFSGLKTALLYHLKNHPADREQHGADLAAAYQEAVMDSLAARVARALSQTGAR
                     TLACVGGVAKNAVLRGKLEALARRHQARLLLTPLAFCTDNAAMVAAAAGLRVLAGLDN
                     PPAREADPSLPLVAEEENP"
     gene            complement(5199..6554)
                     /locus_tag="PKI64_08835"
     CDS             complement(5199..6554)
                     /locus_tag="PKI64_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOE01149.1"
                     /translation="MKTFARNYKWLLLTLVLALNLAVGARLYSQETAAEKPPKDSPLE
                     MYNLFSKVVEQVRANYVDADKSNYQDLIYGALRGMLQSLDPHSTFMDEEAFTAMREDT
                     AGKFGGLGITIGIKDNLLTVIAPMEGTPAFQAGLYSGDKIVEINGESTDGLALEEAVK
                     RLRGEPGTKVKIKIFRPKTQLMKEFELERAIINVPSVKDTRVLDGGIGYVRMLQFGEE
                     TAADLQTALDELEKEKVKALVLDLRSNPGGLLTAAVDVAQKFLKRGELIVFTRGRDNR
                     MERSYRARARHVFPNVPMVVLINGYSASASEIVAGALQDNRRAILVGEKSFGKGSVQS
                     ILPQDGGTAIRLTTAKYYTPSERVIHDVGIEPDIVVPMSGENWRDILLARNKAEEKNG
                     DDAPPASDEGWMDEEGAAPPPMPKPAATSADVLDLSKVTDPQLDRACDILRGVLVFER
                     R"
     gene            complement(6591..8816)
                     /gene="glgB"
                     /locus_tag="PKI64_08840"
     CDS             complement(6591..8816)
                     /gene="glgB"
                     /locus_tag="PKI64_08840"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOE01150.1"
                     /translation="MRTKPILSTAEIQAIIYGMHGAPFSLLGMHETGAKKPGVVVRVF
                     RPYATQVELIRKSDGQAFDMPRIHEDGLYELFFEDEKPFAYKLRLTWFDKTVAELEDP
                     YRFPLQLTDLDMYLLGEGTNVRAYNKMGAHPLTVDGVEGVHFAVWAPNAIRVSVVGWF
                     NNWDGRIHPMQQRGNSGLWEIFMPHLKPGDLYKFEVKGHQAFLAQKADPYAFASELRP
                     RSASMVWDIQAYQWNDAEWLAQRAQRHWLDEPISVYEVHLSSWRRNPAEQNRMLSYRE
                     LAETLIPYVKKMGYTHIEMLPISEHPFDGSWGYQTIGYYSPTSRFGTPDDFKYFVDQC
                     HQAGIGVIVDWVPAHFPKDGHGLAYFDGTHLYEHADPRLGEHKDWGTYIFNYGRNEVR
                     AFLLSNAMFLADVYHIDGLRVDAVASMLYLDYSRKEGEWIPNKYGGRENLDAVDFLKK
                     FNELVHVEYPGFLTFAEESTAWPMVSRPVYLGGLGFDFKWNMGWMHDTLDYMHKDPIF
                     RKYHHHQMTFSMIYAFNENFILPFSHDEVVHGKGAMLAKMPGDMWQQFANLRLLYAYM
                     FAHPGKKLTFMGMEIGQWREWQHGESLDWHLLQYDSHSGLQRLARDLNEMLKGFACLH
                     QIDFSWEGFEWIDLNDWENSMLFTLRKGKEPDDLMVCGFNFTPVPRQGYRIGVPRPGT
                     YEEVLNTDATIYGGSGMGNPRFVRTERREWQGREHSIPVTVPPLGAVYFRFRPDMNGQ
                     V"
BASE COUNT         1681 a         2978 c         2627 g         1677 t
ORIGIN      
        1 tgacttgacg gcccccatag aagggctgga atggtcgcag accttccagc cgttcacggc
       61 gggaaggttt cattcttcaa ccattcccgc cctacaaaag cccgacaaga accgccacgg
      121 cgcaatgcgg aactcaggtt ttcctttcct gttattctgc atttcccgtt ttcctacttg
      181 gtttttccga tcccgccgcc ggtacaacgg cggcctacat caaatccgca cgtcgtattt
      241 gcaggccagg gttgcacccc ccggcgggcg gttatttcgg gccatgaatt gcccagttta
      301 ttaatttata ggaccgaccc cttcgttttc ccgccctaca aaagcagccg ggtagattgt
      361 ggggctggaa cggtcgcaga ccttccagcc gttcacggcc tgcaaaggcc cccgagaggg
      421 aaactcaaac cagcatcacg ttccagctcg acaactgacg cgcccgcccc acggcgcaaa
      481 cgtcaatacg aatagtagcc gtcgtcttct tcgtattcga tttcgtagcc ttcgtcatct
      541 accggcggca atacaccctc tgccaccaat ttggcgtcgt tttccggagt cagttgcacc
      601 ggcaatggcg gcagattgcc gccgcgcaaa atttccattt ggtattcctg caagtggcgc
      661 tccaactctt cgccggtgta aatgggcttg ggcacttcca cgggcttttg ccgttccagc
      721 atccgctggc gacggcgttc cgcgtaggag gtgcgcagtt cggcggcctg cctgatggcc
      781 tcgcgcccgg cgctggcgga gagcacttgc ccccccgcgg tgtcattcat tgaaagcacc
      841 accacctggc cgtctttttg cagggtggcg ctctccgcat cataatcggc atcctccagc
      901 atgatgccgg tgctgcggtc cttctcgccc accatcaggg aaaagtagct gttgtctttt
      961 ttgtcggtga tggccacgcg caggttgccg tcggccagct catagatgcc ggaaagttgc
     1021 agcgaggccg caaacgattc actgactggc accgtgcgct ccggaatttg ggagggatcc
     1081 ggcggcgcgc cgaacggctt gcggtccagg atcacttgat aatcctcaaa cggtcgcacc
     1141 ggtgcgggag ccgccagcgt cgtggcgcac gcgcccagaa tgccgaggat gaccaaccgt
     1201 ttcatcaacc aactatgtac cgcgccgggc ccgcttgcgc aacagctatc atgcgccccg
     1261 cccaggagtg ctccagatga aggcttcgcg gaaaagaccc tgcacatggc cgcgcaccaa
     1321 caccagatgg ttgcccaacg gtgcggaaag caactcgccg gcggccggcg gcgaaacacg
     1381 caccaccact tgcgtgcggc acaggtccgg cgcgcggcgg ttttcgacca acgtgccctc
     1441 cgcgccccac cagcggtcga gattgctgcc gccgagccgc agcaaggtca ccgggccgga
     1501 tgcaaattcg ccggcaatgc ccaagccaat gcccgactca aagtgcgtgc ggaaacggta
     1561 gccccccacc atgcccagcg gcacggtgca gtgcgccagc aacagctcgc ccttgggcac
     1621 atccacatca gacggattgg ccatccaccc cgcctgcccg gtcagatgcc acagccagcg
     1681 caacaacacg gcgctgggga tatcgccctc gcagccggca gtgatgccgg catcggccag
     1741 cagcgccagc gcgaggcagc cggtcacgcc atctttttcc accaaatcga aacagcgcag
     1801 cgtaagcatc gaaagctggt ggttctgcac cagcgcgcgc aggccgcgcg tcacccgcac
     1861 ggcttgggca aaagcctcgc gcgtgaggcc gtctttgccg tgcgcccgat cccagaggtc
     1921 aaattcgggg ccatcttcgg gcagcggctg ttgcgcgata tacgcacgca attcatccac
     1981 cgcaaactcc ttgacccaca ttccgaagcg gcgcgccacc agttcggcgc gcggcgagct
     2041 ggccacaagc caatcggaac tggggccgat gatcccgaac cggtcctgcg cgaggcggtt
     2101 gttggctgcc agcgcgcgtg cggtttgcgt aatctccgcc aaggtgcggt cgtcctcttt
     2161 gcccttcagc aaaaagattc gcccgggccg cccttcctgt tgaatggccg ccagcatttc
     2221 cagagccgcc ggcagcgaat tatgccccgg gtgggcaatc agcagcagcg ggccgccgcg
     2281 gccctgctgg atgcgcgacc ggtgccggcg cagcacatcg tgctccgtgc cgccggtctg
     2341 cacaaaataa aaaggcacgc cgggcgcgtc ggcaacttcg cccacgccgc ggtgaccacc
     2401 cgcgccaacc agcgcctgct cgtgtccggc cagcagcgcg gacaggctgc gcgcatcgtg
     2461 cagcgccgaa gccaccggga tataccaaaa acttgtcgcg ggggcggtgg acggattcat
     2521 ggcgcaatct tatcgaacga atccacaaac tccaccgccg ggaaaaaggc atccgccttg
     2581 aaaaacgcgg ccgggatttc tccattggcc accgggctgt ccacccgcgt ggtttccgta
     2641 cgctccatgc cctgcaccgt caccttggcc tgctgcaggc aggcaatcca cacgccgggc
     2701 gccacctctt gaaacgcgga gaaatcggtt tgcgtcagca agtccgtcat ggccggcgaa
     2761 ccatacagtt cgaagcgcgc caagcggcct tgtgcatcca gattcagcac ggcaaacgat
     2821 ttctgattgt cgtagcccgc gcgcacggga aattcggcag tgggttccag cggcacttcc
     2881 gtggcgccac gcagcacctc cagcatattg gcggctgagc cgggcaccat gcgcaagtta
     2941 cgcagcatct ccccatccag ctccgccacc gggcgcgaaa accctttcgg cgcgccctcg
     3001 gtgtattgcc ggaaaaccgt gccgtccgaa atggtccgcc gcttgacggg gctgaagttt
     3061 tccacgtgca gcttgtccgg gcgctggtaa tacacgcggc tcagcatccg cagcgtattg
     3121 ccatcgggca agggggtgtc gcgccggatt tcgcagctca tggactgcac ggaatcatag
     3181 ggcgcgagca ctttctccaa cacggtttcc gcgcgcgccc ccgccgccgg cgccaaaacc
     3241 aagatcaaca tcatcctcat ccagcgctgc atggttttcc tccgcaatga acgggaagat
     3301 tcgcacaacc gggcccgtgc ttgcaagccg ggcatggctg cggcatgatg ccgccccatg
     3361 cccctgcccc gcaccatcct gttattgctg cccctgctgc tcaccggtgg cgccgcccgc
     3421 gccatggaac cggagcggct ggagtacgac atcacgtggg tcggcatctc catcggcacc
     3481 atggtggtgc aaaatgagtt ggatgccgac ggccatcccg tgcgcttgct gcgcatgtgg
     3541 aaccggccat ggatcgcgcg gttttatccg gtggatacca ccatcgaatg cagcatcatc
     3601 cccgaccccg acggctcttg ccacgtggtc cacaagcacg tccgcgaaaa tacattccgg
     3661 caagacgaca cgctccaact gtggcccgcg caaggccgcg ccatctggag caaccagctt
     3721 acgcaaactg ccacggaatt caccacgccc actggcgcgc atgatgtggt gacgttcttc
     3781 tttgatttgc gcaacatgct ctccaatgcc cccggccccg ccggcggcga ttatgagctg
     3841 gtgatggacg acggcgttca cggcctgacc atccacatgg gcgaagccca gcgcatccgc
     3901 acgcccttcg ggcgggtgga agccgtccaa gtgaaggcca tctccaagtc gccaacgcta
     3961 ttctcgcgca accggcccaa ggctgtgtgg gtggccacca gtcaaccagt ggtgttgttt
     4021 gcggatgtgg attcgcgctt tggcgcggtg cgtgccacgc ttacccaatg gacgcgcggc
     4081 aacaagcccg tgcaagcgga gtaacgcggg ccgacttacg gattttcttc ttccgccacc
     4141 agcggcaggc tgggatcggc ctcccgagcc ggtgggttgt ccaaccccgc cagaacccgc
     4201 agaccggccg ccgccgccac catggcggcg ttatcggtgc aaaaggccag cggggtcaac
     4261 agcagccgcg cctgatggcg ccgcgccagc gcctccagct tgccgcgcag caccgcgttt
     4321 ttcgccacgc cgcccacgca ggccaacgtg cgcgcgccgg tttgcgacaa cgcccgcgcc
     4381 acgcgcgccg ccagcgaatc catcaccgcc tcctgatagg ccgcggctag atcggcccca
     4441 tgttgctcgc ggtcggccgg atgatttttc aggtggtaga gcagcgcggt tttgagcccg
     4501 ctgaaactga aacatagctc gatggccaag ccgtcatccg tggtgcgttg cttggcatgt
     4561 tccagcccgc gcggaaagcg cacaaaaccg gcatcgccgc cgcgcgcggc tttttcgatg
     4621 gccggcccgc cgggatagcc aagccccagc aggctggcgc ctttgtcgag acattcgccg
     4681 gcggcatcgt cgagggtctg gcccagcaat tggtagcgcc ccacgcccgg catcaaaatc
     4741 aggcacgtat ggccgccgga caccatgagc accagcgccg ggcacgcggt ggccggcggc
     4801 ggcgcggcgg aatccaagaa catgcccgcc acatgggctt ccagatggtt cacgccccac
     4861 accggcttat ccagcgtctg ccccagcgcc cgcgcgccgg aaagcccgat gagcagcgaa
     4921 ctgaccaacc ccgggccgtg ggtgacggca atcgccgtca attctttcca cgccacgccg
     4981 gcttgcgtca tcgcggcaac caccacctcg ggaaacttgc tgacgtgctc gcgcgaggca
     5041 atctccggca ccaccccccc ccacggctga tggctggcaa tctggctgtg caccacggag
     5101 gaaagcactt cgcggccgtc gcgcaccacc gccgcagcgg tttcgtcgca cgaagactct
     5161 atgcccaaga tcagcacggc gcgcttcccg cgcaggcgtt agcgacgctc gaacaccagc
     5221 accccgcgca agatgtcgca ggcgcgatcc aactgcggat cggtcacctt gctcaggtcc
     5281 agcacgtcgg cgctggtggc cgccggtttg ggcatgggcg gcggcgccgc gccttcttca
     5341 tccatccaac cctcgtcgct ggccggaggg gcgtcatcgc cgttcttttc ctcggccttg
     5401 ttgcgggcga gaagaatatc gcgccagttt tcgccggaca tcggcaccac gatatccggc
     5461 tcgatgccca catcgtggat gacgcgttcg ctgggcgtgt aatacttggc ggtggtcagg
     5521 cggatggcgg tgccgccatc ctgcggcagg atgctctgca cggagccctt accgaaactt
     5581 ttttcgccca cgaggatggc ccgccggttg tcttgcagcg cgccggccac gatctcggag
     5641 gcgctggcgc tatagccatt gatcagcacc accatcggca cgttggggaa cacatggcgg
     5701 gcccgcgcgc ggtacgaacg ctccatgcgg ttgtcgcgcc cgcgggtgaa cacgatcaat
     5761 tcgccgcgtt tcaggaactt ttgggccaca tccacggccg cagtcagcag cccgcccgga
     5821 ttactgcgca aatccagcac aagcgctttg actttttctt tttccagttc gtcgagcgcc
     5881 gtttgcaggt cggcggccgt ttcctcgccg aattgcagca tccgcacata gccgatgccg
     5941 ccatccagca cgcgggtatc cttgacactc ggcacattga tgatggcgcg ctccagctca
     6001 aactctttca tcaactgggt tttgggccgg aaaatcttga ttttgacttt ggtgcccggc
     6061 tcgccgcgca ggcgcttgac ggcctcttcc agcgccagcc cgtcggtgct ttcgccgttg
     6121 atttccacga tcttgtcgcc ggaatacagc ccggcctgga acgccggcgt gccctccatg
     6181 ggggcgatca ccgtcagcag gttgtccttg atgccgatgg tgatgcccag cccgccgaac
     6241 ttgccggcag tgtcctcgcg catggcggtg aaggcctctt catccatgaa cgtgctatgc
     6301 gggtccagcg actgcagcat tccgcgcagt gcgccataga tcaggtcctg ataattgctc
     6361 ttgtccgcgt ccacatagtt ggcgcgcacc tgttcgacca ccttggagaa aaggttgtac
     6421 atctccagcg gcgagtcctt gggcggcttt tcggcggcgg tttcctgcga atagagccgg
     6481 gcgcccacgg caaggttcag cgccaggacc agcgtcagca acagccattt atagttacgt
     6541 gcgaatgttt tcatgatgtg tggaaagaat acctcagcgg gggttcaatg tcatacttgg
     6601 ccgttcatgt cggggcggaa gcggaaatag accgcgccca gcggcggcac ggtgacgggg
     6661 atggagtgct cgcggccctg ccactcgcgc cgttccgtgc gcacaaagcg cgggttgccc
     6721 atgccgctgc cgccgtagat ggtggcgtcg gtgttgagca cttcttcata ggtgcccggg
     6781 cgaggcacgc cgatgcggta gccctgtcgc ggcacgggcg tgaaattgaa gccgcacacc
     6841 atcaggtcat ccggctcttt gcccttgcgc agcgtgaaga gcatgctgtt ttcccagtcg
     6901 ttgaggtcaa tccactcgaa cccctcccac gagaaatcaa tctggtgcag gcaggcgaag
     6961 cccttgagca tttcgttgag gtcgcgggcc aggcgctgca agccggagtg cgaatcatat
     7021 tgcagcaggt gccagtcgag gctttcgccg tgctgccact cacgccactg gccgatctcc
     7081 atgcccatga acgtcagctt cttgcccgga tgcgcgaaca tataggcata gagcaaccgc
     7141 aggttggcga actgctgcca catgtcgccc ggcatcttgg ccagcatggc gcccttgccg
     7201 tgcaccactt catcgtgcga gaacggcaaa atgaagtttt cgttgaaggc atagatcatc
     7261 gaaaacgtca tctggtggtg gtgatatttg cggaagatgg ggtccttgtg catgtagtcg
     7321 agcgtgtcgt gcatccagcc catgttccac ttgaagtcga accccagccc gcccaaatac
     7381 accggccgcg acaccatcgg ccacgccgtg gattcttccg caaacgtcaa gaagccggga
     7441 tattccacgt ggaccagctc gttgaacttc ttgaggaaat ccaccgcgtc gagattttcc
     7501 cgcccgccgt acttgttggg aatccactcg ccttccttgc gggaataatc gaggtagagc
     7561 atggaggcca ccgcatccac gcgcaggcca tcaatgtggt acacatccgc caaaaacatg
     7621 gcgttggaga gcaggaaggc gcgcacttcg ttgcggccgt agttgaagat ataggtgccc
     7681 caatccttgt gctcgccgag gcgcggatcg gcgtgttcat agaggtgcgt gccgtcaaaa
     7741 taggccagcc cgtggccatc cttcgggaag tgcgccggca cccagtccac aatgacacca
     7801 atgccggcct gatggcattg gtccacgaaa tacttgaaat cgtccggcgt gccgaagcgc
     7861 gaggtcggcg aatagtagcc gatggtctgg taaccccagg aaccatcgaa cggatgctcg
     7921 ctgatcggca gcatctcgat gtgcgtgtag cccatctttt tcacgtaggg gatcagcgtt
     7981 tccgccagct cgcggtagct caacatgcgg ttttgctcgg ccggattgcg ccgccacgag
     8041 gagaggtgca cttcgtacac actgatcggc tcatccaacc agtgccgctg cgcgcgctgc
     8101 gccaaccatt ccgcatcgtt ccactgatag gcctggatat cccacaccat ggaagccgac
     8161 cgcgggcgca gctccgaggc aaaggcatac ggatccgcct tctgcgcgag gaaagcctga
     8221 tggcccttga cctcgaactt gtacaaatcg cccggcttga ggtgcggcat gaaaatttcc
     8281 cacagccccg agttgccgcg ctgctgcatg gggtggatgc gcccgtccca gttgttgaac
     8341 cagcccacca cgctcacgcg aatggcgttg ggcgcccaca cggcaaaatg caccccctcc
     8401 acgccgtcca ccgtcagcgg gtgcgcgccc atcttgttgt aggcgcggac attggtgccc
     8461 tcgcccagca aatacatgtc cagatcggtc agttgcagcg gaaaccggta cgggtcttcc
     8521 agctcggcga cagtcttgtc gaaccacgtc agccgcagtt tgtaggcaaa cggtttttca
     8581 tcctcaaaaa acaactcgta gagcccgtcc tcatgaatcc gcggcatatc gaaggcctgg
     8641 ccgtcgcttt tgcggatcaa ctccacctgc gtggcatagg gccggaaaac ccgcacgacc
     8701 acgcccggct ttttcgcccc cgtttcgtgc atgcccagca gcgaaaacgg cgccccgtgc
     8761 attccataga tgatcgcttg gatttcggcc gtggataaaa ttggttttgt gcgcataatc
     8821 ctctcccggg ccggaacatc gccagacgcg ccagcctccc gtcataatcc cacagcgccc
     8881 cgcccgccgt aaaggctgaa aggttcgcgg ggtgcacgaa ataaaagttg gttaagtgaa
     8941 aatctctctt tttttggggg aaa
//
LOCUS       DAOPNP010000103         8925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_8803_length_8925_cov_5.718715, whole genome shotgun sequence.
ACCESSION   DAOPNP010000103 DAOPNP010000000
VERSION     DAOPNP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8803_length_8925_cov_5.718715"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKI64_08845"
     CDS             complement(<1..542)
                     /locus_tag="PKI64_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01151.1"
                     /translation="MKMKQRLVCGITAGLFLFGGYAAAAGHPLLTLQDSSLAWAMSDA
                     PLGYGDYGAPSAAAPMTVDATYPLLMAAATPVPASTLALQADTTAVSVGAGQEFSTWA
                     DLTAVFRPSRWRLPIRVGEPLSFLNWRAWHDAPGRTAKVFAGVVVVVGITAAIASAGG
                     GGGGGGGDSGSRDPGVRPPNS"
     gene            complement(569..1612)
                     /locus_tag="PKI64_08850"
     CDS             complement(569..1612)
                     /locus_tag="PKI64_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01152.1"
                     /translation="MIRRLLVLLCLAVVVLALWPVAIHLRDNQPVVLLPGTEEELRER
                     AAAQAATIEQSDADFDQSIPEAALEPQVVMDADETPWPADTATDSIPFAALHKALAAA
                     MAENELAGVQQLLDSLATPAERRGAFRWLAHHDGDAGLAMALTILWMTPADDPRHADY
                     ISALHGIRHPALAAWLVQALATTTDEQTRERLAAVLATVRGPALIEAIEQQLATNYSP
                     TQRAPWLAVLRQRTDFEELPALMSLAESSDPDIAAAAAHGIANIGGWEACLWLAECAL
                     QPNAPHYYRDALAAGSSGFSQAALAFIAGGRGVPEPLRTAAVAGGTATGAGQLHPAVQ
                     PGREGMDDSERWF"
     gene            complement(1609..5502)
                     /locus_tag="PKI64_08855"
     CDS             complement(1609..5502)
                     /locus_tag="PKI64_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOE01153.1"
                     /translation="MNQLPKAWKTMIGQARRWLWLAAVAGIAWGAEPAQAALRPDDVS
                     VQLTAGDAGAVVVEWNSDIPPTAMVGWHVERQLPNGAVTRLTTSWVKVRLLALPGDAY
                     SFVDTTAGARVGDVLTYRLVVVDPEGQEWPGEFRTLAVTPRGAAEPQAEPPARGKSSM
                     QRRGLPIVSGSRIRITVTNDGVYRVTAAEIANLLVGATEAGVATMIQSQGIRLTCNGE
                     AVAWLGEIDGSALRFVGERYSDSYGHENVYWLELAPGLAMAVSNQATALSDTNGWFWE
                     TLRVERQNYYQPQLPGGVEDDYFVWDGVSLTSPESSANLANTITLPDAYSNLKTGTVS
                     VVLIGAYDGPAELDNHTRLRAGGVLVAEQQWKGDERIVLSGPTTNLGGTSLTVRVDIL
                     RDSGVVTTTVMLDALEVTYARHLRARSNQLLFTARPGTNVLTVRGFTQPDIRVFDLTN
                     PRRPVAVQGTVTPDGAAWQISWSVAESDARRYLASAVDLTPAHMTGAAGVTWRKPQAG
                     GKYVVITSYALTNAAQALVDYRQQTGLNSVLVPIEDLFNEFTYGRRDPTAVREFLKYT
                     SQNWTEKPAYVCLAGDGHQDYHDIFNQSATRPNHIPPVKSRVPVDATPSGNNLTVGLD
                     NPMADWKGNAAPDVRLGRLPAQTPAALSNMIARIIQHEAESTWKKSVLLVSDKDENNA
                     FAHAANRLAAKLPPGLTVKKLPLGMTTPVAEMRANFIQQLNMGPFLASYYGHANNFGI
                     SSPYFFQYNATNQIDMLSLTNQVQTPLLVAGTCMLNNFGEPHPNTRCLGKAFLTAAPG
                     GPVAIWSSATENTLSMAEATAGAIVSNLFGADESFLGDVIQNALDLQARSASPWTVAA
                     SVLMGDPALRMRPRRFDDLTPPNLNITTRPGASSASNRVALSGTAADFSGVAEVIVRN
                     NRDVGEWVATGTGSWSVNDIPLHQGVNFLDVIATDIYGNATTQTVKVTYSGDAYYDAG
                     LRSGQVVQAIEFPDNIPRGNTVRVRWQILSYVPVRSYLQTMHRDGTNVVWNVRRNGEL
                     VGQVESSWNVGSGAKKAKVYSFECDWTAPDSGDAPVDIFAWFNVAQMDGVRYLLANIP
                     DGVDNRPDPDIAKVFRRTLVSHASSSSPGTPGGFTEDQSPVREQNPQREFDNINDIKK
                     RSGGVVTAINIPDNWVPGTTVTCQWTVLAYMDIEGQLAAADFANSTIPLQVNGSRVSS
                     KKSTFNFTHNGTTYYAMEYTFRALVNVLAEWNTTPPGEPGVPRVQIFFRHRIQNSNGT
                     RMACNLASGVDARPYASDGMFGRFIERTIQVSPGP"
     gene            complement(5823..>8925)
                     /locus_tag="PKI64_08860"
     CDS             complement(5823..>8925)
                     /locus_tag="PKI64_08860"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOE01154.1"
                     /translation="TDVNGNYIFEDLVPGDYTVHFQRPSGYKPSPQDRGNDDAVDSDA
                     DPTSGQTTTITLTSGQEDLTWDAGFYRPASVGDYVWLDENWDGVQDAGEAGIPNVRVE
                     LLDSNNVVIATTVTDLNGLYLFTDLPPGDYTVRVDLSSLPPQLAANPTYDFDGNRDSI
                     TPVTLQSGDDERRIDFGYNWNPDDVILGSIGDRVWVDTDSDGVQDPGEIGIPDVVLNL
                     YVDDGTGSYTTLVATTTTDASGFYIFPNLPPNAYVVRVDTTTLPPGYVQTGDPDHFGA
                     DASTNPSQAGDNQTTTPIVLAPGDVFVNADFGYVPPAPLSNLGDLIYFDANADGVFNP
                     ADGDYAIPGVTVVLLDSLGNVIASTVTTGDHSATNNYLFTDLPAGTYTVWVNDTDNVL
                     NGLVQTGDPDGGMDSRSTTTLDGIHDDLLQDHGYTPDGHEPLKGLIGDTVFLDRDNDG
                     LPGPGEGIQGVTVRLYDDEGNLLATTVTDANGNYWFGGLDAGRYEIVVDTTTLPNGGA
                     GLVNTVDPDTPNPGDSRSVYDLDQGEINLDQDFGYVTDIPNAIGGTIWRDCNADGILD
                     PGEPYRMAGVGVVLRDSNGNVVGKAFTDADGDFLFEGLPDGTYTVDVVDTANILLGWW
                     HSLGPDQNADKHSKTDPYTVSVAGGEINTTADFGYYLVIAEVGDYVWYDINGNGIQDG
                     GEPGLANVRVRLALEYPDGSVINMETLTDSTGYYLFGNLLLDARYAGSTTNDPAATPD
                     RIRFTISVDGTQDVLVDDGYLPTGVDMGDGTNDSREHSGTFANVTRCSRITVYDFGYS
                     GGPLLAVIGNVDAFTRDGQTIVRWETIESWDTAGFWLEREVNGAWVRISEEMIRYPLF
                     GVAPIVFEQVDPTAVAGETYRYRIVELEKSGALLTYGPYTLTVDGAGHTYEDWAAGQF
                     SAAELLDPAISGRDADPDGDGLTNWQEFLAGTDPLNADSVLRVVDVSHVAGGTELRWN
                     SVPGRSYRIAVADSMGGEFLPVSLAIEATGAVTTYVVPSNDQRLFFQVILVTEGIPGD
                     SEP"
BASE COUNT         1716 a         2844 c         2632 g         1733 t
ORIGIN      
        1 gaattgggag gccgcacacc gggatctcgt gagccgctat ctccaccccc accaccaccg
       61 ccgccaccgg cactggcaat cgcggcggta atacccacga caaccaccac cccggcaaac
      121 actttggcag tacgccccgg agcgtcgtgc cacgcacgcc agttcaaaaa ggacagcggt
      181 tcacccaccc ggatcggcaa gcgccagcgc gaagggcgaa ataccgcggt cagatcggcc
      241 cacgtgctaa actcctgccc ggcgccaacg gataccgccg tggtgtccgc ttgcagtgcg
      301 agggtgctgg ccggcacagg cgtagcggca gccatgagca gcggataggt tgcatctacg
      361 gtcattggcg cggcagccga gggcgcgcca taatcaccat atcccagcgg tgcatcggac
      421 atcgcccacg caaggctcga gtcctgcaac gtgagtaacg gatgaccggc cgctgccgca
      481 tagccgccga acaggaacag tccggccgta attccacaga ccagcctttg tttcattttc
      541 acgatttttc ctttgcgcaa tgcaatcact aaaaccaccg ctcactgtca tccatgcctt
      601 cacggccggg ctgcaccgcc gggtgcaact gcccagcccc agtggccgtt ccaccagcca
      661 ccgctgcggt gcggagcggc tccggcacgc cacgcccgcc ggcaataaat gccagcgccg
      721 cttggctgaa tccggacgaa ccggccgcca aggcatcccg atagtagtgc ggcgcattcg
      781 gctggagcgc gcattccgcc aaccagaggc aggcctccca cccgccgata ttggcgatgc
      841 catgcgccgc cgctgcggcg atatcgggat ccgacgattc cgccagactc atcaaagcag
      901 gtaactcctc aaaatcggtg cgctggcgca aaacggccag ccaaggtgca cgttgcgtcg
      961 gagagtaatt ggtggccaac tgctgttcga tagcctcgat aagagccggc ccacgcaccg
     1021 tggccaacac tgccgcgagg cgctcccgcg tttgttcatc cgttgtggtt gccaacgcct
     1081 gcaccaacca cgcggccaaa gccgggtggc ggatcccatg caacgcgctg atataatcgg
     1141 catggcgcgg gtcatcagca ggggtcatcc acaggatggt caaagccatg gcaaggccgg
     1201 catcgccatc atgatgcgcc agccaacgaa aggccccgcg ccgttccgca ggcgtggcca
     1261 gcgaatccag cagttgctga acaccggcca gttcattttc tgccatcgct gcagccagcg
     1321 ccttgtgcaa ggccgcgaac ggaatggaat cagtcgctgt gtccgccggc catggtgttt
     1381 catcggcatc catgaccact tggggctcta acgccgcttc cgggatgctc tggtcaaaat
     1441 ctgcgtcaga ctgctcgatg gtagcagcct gcgccgcagc gcgctcccgc agttcttcct
     1501 cggtgccggg cagaagcacc acaggctggt tatcccgcag atggatggca accggccaaa
     1561 gcgctagcac gaccacagcc aagcaaagca aaactagaag gcgacggatc atggcccggg
     1621 actcacctga atggtgcgtt caatgaagcg gccaaacatg ccatcgctgg cataggggcg
     1681 tgcatccacg cccgaggcca gattgcaggc catgcgggta ccgttgctgt tttggatccg
     1741 gtgccggaag aaaatctgca cccgggggac accgggctcg ccgggcggtg tggtattcca
     1801 ttccgccagc acattcacca aggcgcggaa ggtatattcc atcgcgtaat acgtggtgcc
     1861 attgtgcgtg aagttgaatg tggatttctt ggacgacacc cggctgccgt tcacttgcag
     1921 cgggatggtc gagttggcga agtcggccgc ggccaactgg ccttcgatat ccatataggc
     1981 caagaccgtc cactggcagg tgacggtagt accgggcacc caattgtccg ggatgttgat
     2041 ggccgtgacc acgccgccag aacgcttctt gatatcgttg atgttgtcga actcacgctg
     2101 cggattctgt tcgcgcacgg gcgattggtc ttcggtgaat cccccgggcg tgccgggcga
     2161 tgaggatgag gcatgcgaca ccagcgtccg gcggaacact ttggcaatat ccggatccgg
     2221 ccggttatcc accccatcgg gaatattggc cagcaggtag cgcaccccgt ccatctgcgc
     2281 cacgttgaac catgcaaaaa tatcaactgg ggcatcgcct gaatcgggtg cggtccagtc
     2341 gcactcgaag gaatagacct tggctttttt ggcgccggaa cccacgttcc atgagctttc
     2401 cacctgcccg accaattcgc cattgcgtcg cacattccag accacgttgg tgccgtcgcg
     2461 gtgcatggtt tgcaagtagg agcgcaccgg cacataggat agaatctgcc agcgcacgcg
     2521 cactgtattg ccgcgtggga tattgtccgg gaactcgatg gcttgcacca cttggccgga
     2581 gcgtagtccc gcgtcatagt aggcgtcgcc actataggtg accttgacgg tttgggtggt
     2641 ggcgttgcca tagatatccg tagcaataac gtccaggaaa ttcacgcctt gatgcagcgg
     2701 gatatcgttc accgaccaag agccggtgcc agtcgccacc cattcgccca catcccggtt
     2761 attgcgcacg atcacttcgg ccactccgct gaagtcggca gccgtgccgg acagtgcgac
     2821 ccggttcgag gcgctgctcg cgcccgggcg ggtggttata ttgagattgg gcggggtaag
     2881 gtcatcgaaa cggcgcggcc gcatccgcaa ggctgggtcc cccatcaaga cactggcggc
     2941 gacggtccaa ggcgacgcgc tgcgcgcctg caagtccagt gcattctgga tgacgtcacc
     3001 cagaaaactt tcatcggcgc caaagagatt gctcacaatg gcaccggcgg tggcttccgc
     3061 catggagagc gtgttttcgg tggccgagga ccaaatcgcc acgggcccgc ctggtgcggc
     3121 cgtcaaaaag gctttgccga ggcagcgggt gttcgggtgc ggctcaccaa agttgttcag
     3181 catgcaggtt ccggcaacca gaagcggcgt ctggacttgg ttcgtcaacg acaacatatc
     3241 aatctgattg gttgcgttat actggaagaa ataggggcta ctgatcccaa agttatttgc
     3301 gtggccataa tacgacgcca ggaagggacc catgttgagc tgctgaatga aattggcgcg
     3361 catctcagcc acaggggttg tcatgccaag cggcaatttt tttaccgtga gacccggtgg
     3421 cagtttggcc gccaaacggt tggccgcatg cgcgaaagca ttattctcat ctttgtcgga
     3481 caccagcagc acgcttttct tccaagtgga ttcagcctca tgctggataa tgcgggcgat
     3541 catgttgctc agcgcggcgg gtgtttgcgc gggcagccgc cccagccgta catccggcgc
     3601 cgcgttaccc ttccagtccg ccattgggtt atccagcccc accgtcagat tgttgccgct
     3661 gggtgtggca tccaccggaa ctcgcgattt gaccggtgga atgtgattgg gacgcgtggc
     3721 cgactggttg aaaatgtcgt gatagtcctg atggccatcc ccagcgagac agacataagc
     3781 tggtttttcg gtccagtttt ggctcgtgta ttttaggaat tcgcgcacgg cggtcggatc
     3841 ccgccggcca taagtgaatt cattgaagag gtcttcaatg ggcaccagca cggagttcaa
     3901 accggtttgc tgccggtaat ccaccagcgc ctgcgctgcg ttggtcagag cataggaggt
     3961 aatgaccaca tactttcccc cggcctgcgg tttgcgccaa gtcactcccg ctgccccggt
     4021 catgtgcgcc ggcgtcagat ccacggcgct ggcaaggtag cggcgggcgt cactctcagc
     4081 cacgctccac gaaatctgcc aagcggcgcc atctggcgtc acagttccct gcaccgccac
     4141 cggccggcgc gggttggtga gatcaaacac acgaatatcc ggttgcgtga agccgcgcac
     4201 cgtcagcaca ttcgttcccg gccgtgcggt aaacagcaat tgattgcttc gcgcccgcag
     4261 atggcgagca taggtcacct cgagcgcgtc caacatgacc gtggttgtaa cgacgccgct
     4321 atcgcgcagg atgtccacgc gcaccgtcag cgaggtccct cccaggttgg tcgttggccc
     4381 cgacaagacg atgcgttcgt cgcccttcca ttgttgttcc gccaccaata cgccgcccgc
     4441 ccgaaggcgg gtatggttgt ccaactccgc tgggccgtca tacgcgccga tcaacacgac
     4501 ggacaccgtt cctgttttga ggttgctgta tgcatccggc agcgtgatgg tgttggccag
     4561 attggcgctg ctttccggcg acgtgagcga cacgccatcc cacacaaaat aatcatcctc
     4621 aactccgccg gggagttgcg gctgataata attttgccgt tcaacgcgca acgtttccca
     4681 aaaccagccg tttgtgtccg acaacgccgt ggcctgattg ctcaccgcca tggccaagcc
     4741 gggagccagt tccagccaat agacattttc atgcccgtag ctgtccgaat accgctcgcc
     4801 cacaaaacgc agagccgagc cgtctatctc accgagccac gccacggcct cgccgttgca
     4861 tgtcagacga atgccctgac tttgaatcat cgtcgccacg cctgcttccg tggcgccaac
     4921 cagcaagttg gcgatttctg ctgcggtcac ccggtacacg ccatcgttgg tgacggtgat
     4981 gcgaatccgg ctgccggaca cgatggggag cccgcgccgt tgcatggacg atttgccgcg
     5041 cgcgggcggt tcggcctgcg gctctgccgc gccgcgcggg gtaacggcga gcgtgcggaa
     5101 ttcgccgggc cactcctgcc cttccggatc cacgacaacc aagcggtacg tcagcacatc
     5161 gccgacccgc gcgcctgcgg ttgtgtccac aaatgaatag gcatcacccg gcagcgcaag
     5221 gagccgaacc ttgacccaac tggtggtgag ccgcgtcact gcgccattcg gtaattgccg
     5281 ctccacatgc cagcccacca tggccgtggg tggaatatcg ctgttccatt ccaccaccac
     5341 cgcgccggca tcgccggccg tcaactgcac ggaaacatcg tcggggcgca gcgctgcttg
     5401 ggccggctct gccccccaag cgattccggc cacggccgca agccacagcc agcgccgcgc
     5461 ctgcccaatc atggttttcc acgcttttgg caactgattc atctccaaca tcaaatcaaa
     5521 aacttatttt tgccggcggt catcttccac aaaaaacatg gacccaaccg cttggccaac
     5581 ggcatcaagc gtttgctcct ctacactacg gcagcacccg cgttttgcca ctgattcact
     5641 cgactttctt tcgacttctt tcccaattca ctgttttgca ttccgaaaca tcaggggcgg
     5701 ccggcttttg ggccgaaaag ttttccacag tgtggaaaaa atatttccac agcgtggaaa
     5761 accgcgcgcc cgcggacccc ggcggctgcc ccggcattcc gtggcagccg ccttggcctg
     5821 gattatggtt cgctgtcgcc cggtatccct tccgttacca aaatgacctg gaagaagagc
     5881 cgctggtcat tactcggcac gacatacgtg gtgacagccc cggtcgcctc aatggccagg
     5941 ctgaccggca agaactcgcc acccatgcta tccgccacgg cgatccggta tgaccgcccg
     6001 ggcacactgt tccagcgcag ttccgtgccg cccgccacat ggctcacgtc caccacgcgc
     6061 agcacggaat ccgcgttcag cggatcggtg ccggcaagga attcctgcca gttggtgagg
     6121 ccgtcgccat ccgggtcggc gtcacgtcca ctgatggccg ggtccagcag ttcggccgcg
     6181 gagaactggc cagccgccca atcttcatag gtgtggccgg cgccatccac ggtcagcgtg
     6241 tacgggccat aagtcaacag tgcgccgctc ttttcgagtt ccacgatccg gtagcgatag
     6301 gtctcgcccg caaccgcagt cgggtccacc tgctcgaaga cgatgggagc tacgccaaag
     6361 agcgggtagc gaatcatctc ttcgctgatg cgcacccagg cgccgttcac ttcgcgttcc
     6421 agccagaagc cagcggtgtc ccacgactcg atggtttccc agcgcacgat ggtctggccg
     6481 tcgcgcgtga acgcatccac attgccgatg actgccagca gagggccgcc actataaccg
     6541 aagtcataga cggtgatccg gctgcagcgg gtcacattgg caaaggtacc gctgtgctcg
     6601 cgcgaatcgt tggtgccatc gcccatatcc acaccggtcg gcaggtagcc atcatccacc
     6661 agcacgtctt gagtgccatc caccgagatg gtgaaccgga tccggtctgg ggtggctgcc
     6721 ggatcgttgg tggtactgcc cgcgtagcgc gcatccaaca gcagattgcc gaacaggtag
     6781 tatcccgtgg aatccgtcag ggtttccatg ttgatgacac ttccatccgg gtattccaag
     6841 gccaggcgca cgcggacgtt cgccaagccc ggctcgccgc cgtcctggat accgttgcca
     6901 ttgatgtcgt accagacgta gtcgcccact tccgcgatca caaggtagta gccgaagtcg
     6961 gccgtagtgt tgatttcacc accggccacc gagacggtgt agggatcggt cttgctgtgt
     7021 ttgtctgcat tctgatccgg gccgaggctg tgccaccagc ccaagaggat attggcggta
     7081 tccaccacat ccaccgtgta ggtaccgtcc ggcagaccct cgaagaggaa gtcgccgtcc
     7141 gcatccgtga aggccttgcc caccacgttg ccgttgctgt cgcgcaggac tacgcccacg
     7201 cccgccatac ggtacggctc gcccggatcg agaatgccat ccgcgttgca atcgcgccag
     7261 atggtaccgc cgatcgcgtt gggaatatcg gtcacatagc cgaagtcttg atcgaggttg
     7321 atctctcctt ggtccagatc atagaccgac cggctgtcgc ccggattcgg cgtgtccggg
     7381 tccactgtgt tgaccagtcc cgcgccgccg ttgggcagcg tggtggtgtc caccacgatt
     7441 tcataacgac cggcatccag cccgccgaac cagtagttgc cgttggcatc ggtgaccgta
     7501 gtcgccagca ggttgccctc gtcgtcatag aggcgcaccg tgacgccttg aatgccttcg
     7561 cccgggccgg gcaggccgtc gttgtcccga tcgaggaaga ctgtgtcgcc gatcaagccc
     7621 ttgagcggct cgtgaccatc cggcgtgtag ccgtggtctt gcagcagatc gtcatggatg
     7681 ccgtccagtg tggtggtgct gcggctgtcc atgccaccat ccgggtcacc cgtctgcacc
     7741 aagccattga gcacgttgtc cgtatcgttg acccagaccg tgtaggtgcc cgcgggcagg
     7801 tcagtgaaca gatagttgtt ggtcgccgag tgatcgcccg tggttactgt gctggcaatc
     7861 acattgccga gactatccag cagcacaaca gtgacgcccg gaatggcata atcaccgtcc
     7921 gcagggttga acacgccgtc cgcgttggcg tcgaagtaga tcaggtcgcc gaggttgctc
     7981 agcggcgccg gcggcacata gccgaagtcg gcgttcacga acacatcgcc cggcgccagc
     8041 acgatgggcg tggtggtttg gttgtcaccc gcctggctgg gattggtcga ggcatcggcg
     8101 ccgaagtggt ccggatcgcc tgtttggaca tagcccggcg gcaaggtggt ggtgtccacc
     8161 cgcaccacgt aagcattagg cggcagattc gggaagatgt agaaaccgga agcatccgtc
     8221 gtggtggtcg ccaccagcgt cgtgtaagat ccggtgccat catcaacgta gaggttcagc
     8281 acgacatcag gaatgccgat ttcgcccggg tcctgcacgc cgtccgagtc ggtatccacc
     8341 cagacacggt cgccgatgga gcccagaatc acgtcatccg gattccagtt ataaccgaag
     8401 tcaatccggc gctcatcgtc gcccgactgc agcgtgaccg gcgtaatgct gtcgcggttg
     8461 ccgtcgaagt cataggtcgg gttggccgcc aactgcggcg gcagactgga gaggtccacc
     8521 cgcacggtat aatcacccgg cggtagatca gtgaacaggt acaacccgtt cagatccgtc
     8581 acggtcgtgg caatcaccac gttgttgctg tcaagcagct ccacgcgcac gttcgggatg
     8641 cccgcttcgc cggcatcctg cacgccgtcc cagttttcat cgagccagac gtagtcgccc
     8701 accgaggccg ggcggtagaa gcccgcatcc cacgtcaggt cttcctggcc gctcgtcagc
     8761 gtgatggtcg ttgtctgccc ggaggtcgga tcggcgtcgc tgtctacggc gtcatcgttg
     8821 ccccgatcct gcggcgatgg cttgtagccg ctcggccgct ggaagtgaac ggtgtaatcg
     8881 cccggcacca ggtcttcaaa gatatagttg ccgttcacgt ccgtg
//
LOCUS       DAOPNP010000104         8624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_9151_length_8624_cov_9.160462, whole genome shotgun sequence.
ACCESSION   DAOPNP010000104 DAOPNP010000000
VERSION     DAOPNP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9151_length_8624_cov_9.160462"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..487
                     /locus_tag="PKI64_08865"
     CDS             98..487
                     /locus_tag="PKI64_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01155.1"
                     /translation="MKYLLDLILVVCVVGLGYLWNGEKKNSRTKSDQIDQLYAAQAKL
                     ELDLKAAQASGEKLAADLAALTETEQQLRQEWEAQTEALAAKTAEADELRQISQKLKE
                     RVDELQGYKDRASKAIIAEKPPPAGQP"
     gene            526..963
                     /gene="ruvX"
                     /locus_tag="PKI64_08870"
     CDS             526..963
                     /gene="ruvX"
                     /locus_tag="PKI64_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOE01156.1"
                     /translation="MSKLLGVDYGDRRTGVAISDAAQLIAFPRETLHCPQLAQAAAAV
                     AKLAEAEQVAAIIVGYPLNMDGSAGPRATRTDEFMAELARRTAIPVRQWDERLSTKSA
                     ESVLIEAGTRRDKRREVVDKLAAQIMLQNYLDAHGPAPDDPAP"
     gene            960..1787
                     /gene="truA"
                     /locus_tag="PKI64_08875"
     CDS             960..1787
                     /gene="truA"
                     /locus_tag="PKI64_08875"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE01157.1"
                     /translation="MSPVPAKGPTPRTRPPRGGQRYRLTIAYDGTDLYGWQTQPGKST
                     VQGALEAALQQLTGESPRVHSSGRTDTGVHARAQVAHLDLARPWEPWRLQKGLNALLA
                     DDIRVTELRRARPTFHARYDARGKEYRYTIWNAPVMSPLHRRQAVHIRAPLAVDAMRE
                     AAAMLEGTHDFAAFSANPHREIEGTVRTLWRLAVRRQGALVTIHAVGDGFLYRMVRSL
                     AGHLLRVGLGAVPAAETATLLASKTRTARVETAPAHGLCLWQIFYGAIPGLPKPPPA"
     gene            1842..2135
                     /locus_tag="PKI64_08880"
     CDS             1842..2135
                     /locus_tag="PKI64_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01158.1"
                     /translation="MADINFNCPHCGQNLNGPEEMAGQTIDCPVCQQSFQIPGGIIEV
                     PKDRMSPAGGRPAPEAAPPSTNDDKGKTVRIELPPEFLAEPEKHVFKIKRIQH"
     gene            2137..3507
                     /gene="argH"
                     /locus_tag="PKI64_08885"
     CDS             2137..3507
                     /gene="argH"
                     /locus_tag="PKI64_08885"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOE01159.1"
                     /translation="MRRKTTVGEVDADVLAYTAGQDRVLDLALVEADCFGTAAHVTML
                     SRMPLQPPPLTPQDARAVIAELRRIMADAAAGRFVITLEDQDVHLAVERRLTEKLGAL
                     GKRVHVCRSRNDQVAVDLRLFAKDRLLAAMAAAAALALALNKFARRHARVPMVGRTHM
                     QPAMPSSVGLWAAAWAESLLDDLALLRTAYDLNNQCPLGSAASYGVPMPIDRHLVSDL
                     LGFARPVANVLHANQARGKLEAIILSALGQAMLTLSRLAQDLILFSMPEFGYFHLPAE
                     FTTGSSIMPQKRNPDVLELLRAKAAKVLAAANLVAEIVRAAPSGYNRDLQEAKAPFME
                     GFDLTISSLQIMAPLVAGLRVDAAALRHGFTPDVFATDRALELVAKGMPFRDAYNEVK
                     NNLQQLAGYSPDEALAAKRHYGAPAGLDFTATDRRIKEFSAWTAREIKRAQACRDRLF
                     ADKTKK"
     gene            3581..3934
                     /locus_tag="PKI64_08890"
     CDS             3581..3934
                     /locus_tag="PKI64_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01160.1"
                     /translation="MTTTTYSFGDYTVALTLDQEAPLGILRDLHICHLGMRFLAHKPL
                     PEFQQYEFDISVRQPAGQDPAKVKCCGIVVSSQPEDDAYRTVIHFADLAHDDRSSLHT
                     LTKNNRMRCADCGNC"
     gene            4047..4649
                     /locus_tag="PKI64_08895"
     CDS             4047..4649
                     /locus_tag="PKI64_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01161.1"
                     /translation="MKKLLVIGMLLGTVTLAGAADDQVFALAAAGEVDAALVADVRAQ
                     LEQMSGAAVRVAEPIALTAEMAMDEIGRAAVKTMQPTDFGIIVIAAAGADAPQGVCLP
                     DEHFGVINVTKLAADATPEQTVRRVMQNALRVESMLVGMSVCPFPLCALTSYETAEDL
                     DHMSGNFCPPCQDRFTRLAGELGLRLVKAEPAAEPAPAAE"
     gene            4661..5935
                     /gene="serS"
                     /locus_tag="PKI64_08900"
     CDS             4661..5935
                     /gene="serS"
                     /locus_tag="PKI64_08900"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOE01162.1"
                     /translation="MLDIKLIRENPDFVRDGLATRNSPVDIAEVLALDERRRAAITEA
                     DQLKNTRNDVSRQIGVLKKAGQDTSEIQRQTREIGEKIAALDNAVREIEAQQEKLMLA
                     IPNLPHASVPRGRDAADNVVVRQWGQPRQFDFAPQDHVAIGERLGLFDFDRAARIAGA
                     GFPLFTGPGARLERALIQFMLDMHTTQHGYTEIAPPHLVTTAAMTGTGQLPKMADDMY
                     HASRDDLWLIPTAEVPVTNLYRDEIIRAPLPLKYVAYTPCFRREAGSAGKETRGLIRV
                     HQFDKVELVKFVPPETSYDELEALVRDVEHVLQTLELPYRVLQLCSGDMSFAAAKCYD
                     IELWAPGHNGWLEDSSCSNFEAFQARRANIRWKNAHGKTEFVHTLNGSGLALSRLYVA
                     LLENGQQADGSVVLPAAIRPYMGGLERLGPPA"
     gene            5944..>6784
                     /gene="tilS"
                     /locus_tag="PKI64_08905"
     CDS             5944..>6784
                     /gene="tilS"
                     /locus_tag="PKI64_08905"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE01163.1"
                     /translation="MLATVQNTLATRLPPLRGKRVLVAVSGGADSVALTLALHQLGVK
                     LVLAHVNHGIRGRAAAADARFVAALARRLGLPLVTGKFSVPQERRPGESLEMAARRIR
                     RDFLLRTAQRRNLHLIATGHTADDQAETLLLRIARGTSVSGLAGIRHSTRHGNITFIR
                     PLRDATRQQVEAFLRQQKQRWREDATNTDDFALRNRVRHEILPLLAARLNPNIHAALL
                     RLGEIAAAEDDFMAGLARRAPRSAKTPLALRRRQALAALHRAGADPAAVDFAAVDQFS
                     TAWK"
     assembly_gap    6785..6884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6885..7378
                     /gene="tilS"
                     /locus_tag="PKI64_08910"
     CDS             <6885..7378
                     /gene="tilS"
                     /locus_tag="PKI64_08910"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE01164.1"
                     /translation="AKKSSTFPQRGKKVHPAWAGFSIVWKNPGTFSTAWKTIFHSVEN
                     SAVPRTLRLRHGRGFTRRLDEVCLSATAVGGRDLIFRNWRAGDRMQPLGMAGSKKLSD
                     IFTDLKVPRDQRAAWVVVECGGEIAALAGWRVARAFAVPGPRAPALRIRIQPMMPRAR
                     SSK"
     gene            complement(7342..7959)
                     /locus_tag="PKI64_08915"
     CDS             complement(7342..7959)
                     /locus_tag="PKI64_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE01165.1"
                     /translation="MTMNLPFRGQSNRRLFFFDTETTGLPRDWNAQLHPRTLNNWPRM
                     IQLAWILCDDDGNELSACTTIIRPEGFVVPPDAARVHGITTEMAMKQGRPLLEVLDEV
                     LPAIEQATFAIAHNIAFDEKILGAEFMRQGRPTPFLKKPMHCTMKEATQFCAIPGHYG
                     FKYPNLNELYRKLFKKSVERAHDAGVDVRACKEIYFELRARGIMG"
     gene            8089..>8624
                     /locus_tag="PKI64_08920"
     CDS             8089..>8624
                     /locus_tag="PKI64_08920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE01166.1"
                     /translation="MDLPALQLSVVIVNWNTRELLRACLESVRQHTGGITYEIIVVDN
                     ASHDGSPAMVRQQFPDVRLIENTENLGFAAANNQAIRMARGNAVILLNSDTELVENSL
                     RILHDFLFSRDSIGAVGGRLIYPGGAPQWSYGFAPSLGRMLWITLSGFLRIQRGRKPG
                     AVIPQADEPAHPVEYIVGA"
BASE COUNT         1604 a         2974 c         2456 g         1490 t
ORIGIN      
        1 ggcttagctc agttggcaga gcagctgatt tgtaatcagc aggtcatcgg ttcgagtccg
       61 atcgccggct ccatggcgag ggcaaaggaa caaacacatg aagtacctgt tggatttgat
      121 cttggtggtg tgtgtggtgg ggctggggta tttgtggaat ggcgagaaga aaaacagccg
      181 cacgaaaagc gaccagattg accagcttta tgccgcgcag gccaagttgg aactggatct
      241 gaaagcggcg caggcttccg gcgagaagct tgccgcggac ttggccgcgc tgacggaaac
      301 cgaacagcaa ctgcgccaag aatgggaggc acaaacggaa gcgctggcgg ccaaaaccgc
      361 agaggcggat gagttgcgcc aaatcagcca gaagctcaag gagcgcgtgg acgagctgca
      421 aggctataaa gaccgggcca gcaaggcgat cattgccgaa aagccgccgc cggccggcca
      481 gccgtaattt gctgcgcccg gatagcgggt tgaggagcgc gcgaagtgtc caaactgctg
      541 ggcgtggatt atggcgaccg ccgcacgggg gtcgcgatta gcgatgctgc ccaactgatt
      601 gccttcccgc gcgagacgct gcattgcccc cagttggccc aagccgcagc cgccgtggcc
      661 aagctggcag aggccgaaca agtggccgcg atcatcgtgg ggtatccgct gaacatggac
      721 ggttccgccg ggccccgcgc gacgcggacc gatgaattta tggctgagct ggcgcggcgc
      781 accgccattc cggtgcgcca gtgggatgag cggttgagta ccaagtcggc cgagtcggta
      841 ttgatcgaag ccggcacccg ccgcgacaag cgccgggaag tggtggataa attggctgcg
      901 caaatcatgc tgcaaaacta tctcgacgcg cacggccccg cgccggacga tcccgcgcca
      961 tgagtcccgt gcccgccaaa gggcccacgc cacgcacccg cccgccgcgg ggcgggcagc
     1021 gctatcgcct gaccatcgcc tacgacggca ctgatcttta cggctggcaa acgcagccgg
     1081 gcaaatccac cgtgcagggc gcgctggagg cggcgttgca gcagcttacc ggcgaatcac
     1141 cgcgcgtcca ttccagcggg cgtactgata ctggcgtcca cgcccgcgcg caggtggcgc
     1201 acctcgattt ggcgcgcccg tgggaacctt ggcgtttgca aaaaggcctg aatgcgctgc
     1261 tggcggacga catccgcgtg acggagttgc gccgcgcgcg cccaaccttc cacgcccgct
     1321 acgacgcacg tggcaaggag tatcgctaca ccatctggaa cgcgccggtc atgtcgcccc
     1381 tgcaccggcg gcaggcggtt catatccgcg cgccgctggc cgtggatgcc atgcgcgagg
     1441 cggccgccat gctggagggt acgcacgact ttgccgcgtt ttccgccaac ccccaccgcg
     1501 aaatcgaagg caccgtccgc acgctgtggc ggttggccgt gcgccggcag ggggcgctgg
     1561 tcaccatcca tgccgttggc gatggcttcc tctaccggat ggtccgcagc ttggccggtc
     1621 atctgctgcg cgtgggactt ggcgcggtgc cggcggccga aaccgccacg ctgctggcca
     1681 gtaaaacccg caccgcacgc gtggagactg cccccgctca cggcctctgc ctgtggcaaa
     1741 ttttctacgg cgcaattccg gggcttccca aaccgccgcc ggcgtgatag ggtacttgca
     1801 caattcagga gtggcgcagc cgaaaacgcg aggaaacgga catggcagac atcaatttca
     1861 attgccccca ctgcggacaa aacctcaacg gcccggagga aatggccggc cagaccattg
     1921 attgcccggt ctgccagcaa agtttccaaa tccccggggg gatcatcgaa gtgcccaagg
     1981 accgcatgag ccccgccggc ggtcggcccg cgccagaagc cgcgcccccg tccaccaacg
     2041 atgacaaagg caaaaccgtg cgcattgagt tgccgccgga atttctcgcg gaacccgaga
     2101 aacacgtgtt caagatcaag cgaatccagc actaggatgc gccgcaaaac gacggtgggg
     2161 gaggtggatg cggatgtgct ggcctacacg gccggccaag accgcgtttt ggatttggcg
     2221 ctggtggagg ctgattgctt tggcactgcc gcgcatgtca ccatgctttc ccgcatgccg
     2281 ctccagccgc cgccgctaac cccgcaagac gcgcgcgcgg tgatcgcgga gctgcgccgg
     2341 atcatggccg atgccgctgc cgggcggttt gtcatcaccc tcgaagatca ggatgtccat
     2401 ctggcggtgg aacgccggct gacggaaaag ctcggtgcgc tcggcaagcg cgtgcacgtg
     2461 tgccgcagcc gcaacgacca ggtggctgtg gacctgcgct tgttcgccaa agaccgcctg
     2521 ctggcggcca tggcggctgc tgcggcgttg gcgcttgcgc tcaacaaatt tgcccgccgc
     2581 cacgcgcgcg tgccgatggt cggccggacg cacatgcagc cggccatgcc gtcgagcgtt
     2641 ggcctgtggg ccgccgcttg ggccgaaagc ctgctcgatg atctcgccct gctgcgcacg
     2701 gcttacgacc tgaacaacca atgcccactc ggttccgccg ccagctacgg cgtgcccatg
     2761 cccattgacc gccatttggt cagcgacctg ctcggcttcg cccgcccggt ggccaacgtg
     2821 ctgcacgcca accaggcccg cggcaagttg gaggccatca ttctctccgc gctgggccag
     2881 gccatgctga ccctctcccg gctggcgcag gacctcatcc tcttttccat gccggagttc
     2941 ggctacttcc acctgccggc ggaattcacc accggcagca gcatcatgcc gcaaaagcgc
     3001 aacccggatg tgctggaact cctgcgcgcc aaggccgcca aggtgctggc cgcggccaac
     3061 ctcgtggccg aaatcgtgcg cgccgccccc tccggctata accgcgattt gcaggaagcc
     3121 aaggccccct tcatggaagg cttcgacctg acgatttcat cgctccaaat catggccccg
     3181 ctcgtcgccg gcttgagggt ggatgccgcc gcgctgcgcc acggtttcac ccccgatgtc
     3241 tttgccaccg accgcgcgct cgaactggtc gccaagggca tgcccttccg cgatgcctat
     3301 aacgaagtca aaaacaatct ccagcaactt gctgggtata gtcccgatga agccctcgcc
     3361 gccaagcgcc attacggcgc gcccgcaggc ctcgatttca ccgccactga ccgccgcatc
     3421 aaggaattta gcgcctggac cgcccgcgaa atcaagcgcg cgcaggcctg ccgcgaccgc
     3481 ctctttgcgg acaaaacaaa aaagtagccg gagccgttgc atgtcatcca aagtgggcta
     3541 tctttcactc atcggcgggg gaagacagaa taaagatgac atgacaacaa caacctattc
     3601 atttggcgat tacaccgtcg ccctgacgct cgatcaagaa gctcccctcg gcattttgcg
     3661 tgacttgcat atttgccatc tcggcatgcg ctttctggcg cataaaccgc tccccgaatt
     3721 ccagcagtac gaattcgata tttccgtccg gcagcccgcc ggccaggatc ccgccaaagt
     3781 gaagtgctgc ggaatcgtcg tcagctccca gcccgaagac gatgcctacc gcaccgtgat
     3841 ccactttgct gatctggcgc acgatgaccg ctccagcctc cacacactca ccaaaaataa
     3901 tcgcatgcgc tgcgccgact gcggcaactg ttagttccgc ccgcgctcgc ttttccacgg
     3961 acttcccacg gccgccccgc aaatcgggtt gcggccggtg tcattttggc ctataagcat
     4021 cgcgtctgaa tcgaggagat tttgccatga aaaaactgtt ggtgatcgga atgctgttgg
     4081 gcaccgtcac gctggctggt gcggcggatg accaggtgtt tgctctcgcg gccgccggtg
     4141 aagtggatgc cgcgctggtg gccgatgtcc gcgcgcagct cgaacaaatg tccggcgctg
     4201 ccgtgcgcgt cgccgagccc atcgccctaa ccgccgaaat ggccatggat gaaatcggcc
     4261 gcgctgctgt caaaaccatg cagccaaccg attttggcat catcgtcatt gccgccgccg
     4321 gcgccgatgc cccccaaggc gtctgtctgc ccgatgaaca ttttggtgtc atcaacgtca
     4381 ccaagctggc cgccgatgcc acgccggagc aaaccgtgcg ccgcgtcatg caaaacgccc
     4441 tgcgcgtgga aagcatgctg gtgggcatga gcgtgtgccc attccccctg tgcgcgctca
     4501 ccagctacga aaccgcggaa gacctcgacc acatgagcgg taatttctgt ccgccctgcc
     4561 aggaccgctt cacgcgcctc gccggcgaac tgggcctccg ccttgtgaag gcggaacccg
     4621 ccgccgaacc cgcccctgcc gctgaatagg cgctctcccc atgctcgata tcaagctcat
     4681 tcgggaaaat cccgattttg tccgcgacgg ccttgccacc cgcaactcgc cggtggatat
     4741 cgccgaagtc ctcgccctcg atgaacgccg ccgcgccgcc atcaccgagg ccgaccaact
     4801 caaaaacacc cgcaacgatg tctcccgcca aatcggcgtg ctgaaaaaag ccgggcagga
     4861 caccagcgaa atccaacgcc aaacccgcga aatcggcgag aaaatcgccg cgctggataa
     4921 cgccgtccgc gaaatcgaag cccagcagga aaagctgatg ctcgccatcc ccaacttgcc
     4981 ccacgccagc gtgccgcgcg gccgtgacgc cgccgataac gtcgtcgtgc gccagtgggg
     5041 ccagccccgc caatttgatt ttgccccgca agaccacgtg gccattggcg aacgcctcgg
     5101 gctcttcgac ttcgaccgcg cggcgcgaat cgccggcgcg ggcttcccgc tgttcaccgg
     5161 gccgggcgca cgcctcgaac gcgcgctcat ccagttcatg ctggatatgc acaccaccca
     5221 gcacggctat accgaaatcg ccccgccgca tctcgtcacc accgccgcca tgaccggcac
     5281 cggccagttg cccaaaatgg ccgacgacat gtatcacgcc tcccgcgacg atctctggct
     5341 gattcccacc gccgaagtcc ccgtcaccaa tctctaccgc gacgaaatca tccgcgcccc
     5401 cctgccgttg aaatacgtcg cctacacccc ctgcttccgc cgcgaggccg gttccgcggg
     5461 caaggaaacc cgcggcctga tccgcgtgca ccaattcgac aaagtcgagc tggtcaaatt
     5521 tgtcccgccg gaaacctcct acgacgaact cgaagccctc gtgcgcgatg tcgaacacgt
     5581 tctgcaaacg ctggaactgc cttaccgcgt cctccagctc tgctcgggcg atatgagctt
     5641 cgccgccgcc aagtgctacg acatcgaact ctgggccccc ggccacaacg gctggctgga
     5701 agattcttcc tgcagcaatt tcgaggcgtt tcaggcgcgc cgcgccaaca tccgctggaa
     5761 gaacgcccac ggcaaaacgg aattcgttca caccctcaac ggctccggac tcgcgctctc
     5821 gcggctctat gtcgccttgc tggaaaatgg ccagcaggcc gatggctccg tcgtgctgcc
     5881 cgccgccatc cgcccctata tgggcggact ggaacgcctc ggaccgccgg cctaaacccg
     5941 cccatgctgg cgacggtcca gaacaccctg gccacgcgct tgccgcccct gcgcggcaag
     6001 cgcgttttgg ttgccgtcag cggcggcgcg gattccgtcg cgttgacgtt ggccctgcac
     6061 caactgggcg tgaaactggt cctcgcccac gtcaaccacg gcatccgggg ccgcgccgcc
     6121 gcggccgatg cccgatttgt cgccgctctc gcccgtcggc tcggcctgcc gctggtcacc
     6181 ggcaaatttt ctgtcccgca ggaacgccgt cccggcgaat cgttggaaat ggccgcccgc
     6241 cgcatccggc gagacttcct cctccggacc gcgcagcgcc gcaacctgca cttgattgcc
     6301 accggccata ccgccgacga ccaagccgaa accctgctgc tccgtatcgc gcgcggcaca
     6361 tccgtttccg gcctcgccgg cattcgtcat tccacccggc acggcaacat cacgttcatc
     6421 cgcccgctgc gcgacgccac ccgccaacaa gtagaagcat ttttacgcca acaaaaacaa
     6481 cgatggcgcg aagatgccac aaacaccgac gacttcgccc tgcgcaaccg cgttcgccac
     6541 gaaatccttc cgctccttgc cgcgcgactc aacccgaata tccacgccgc cctgctgcgc
     6601 ttgggcgaaa tcgccgccgc cgaagatgac ttcatggccg gcctcgcgcg gcgcgccccg
     6661 cgttccgcca agaccccctt ggccctccgc cggcggcagg cgctcgccgc gttgcatcgc
     6721 gccggtgccg atcccgccgc tgtggatttc gccgccgtgg accagttttc cacagcgtgg
     6781 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgcca aaaaatcgtc
     6901 aacttttcca cagcgtggaa aaaaagttca tcccgcatgg gcgggatttt ccatagtgtg
     6961 gaaaaatccc ggcacttttt ccacagcgtg gaaaactatt ttccacagtg tggaaaactc
     7021 cgccgtcccc cgcaccctgc gcctgcgcca cggccgcgga ttcacgcgcc ggctggacga
     7081 agtctgcctg tcagccacgg ccgtcggcgg acgcgatttg atcttccgca actggcgtgc
     7141 cggcgaccgc atgcagccgc tgggcatggc cggcagcaaa aaactgtccg atatttttac
     7201 tgacctcaaa gtgccgcgcg accaacgcgc cgcgtgggtg gtggtggaat gcggcggtga
     7261 aatcgccgcg ctggccggct ggcgggtggc gcgggctttc gccgtgcctg gaccccgtgc
     7321 gcccgcgctg cggatccgaa ttcagcccat gatgccgcgc gcgcgcagct cgaaatagat
     7381 ttccttgcag gcgcggacgt ccacccccgc gtcgtgtgcc cgctccaccg acttcttgaa
     7441 cagcttgcgg tacagctcgt tcagattcgg atacttgaag ccgtagtgcc ccgggatcgc
     7501 gcaaaactgc gtggcctctt tcatcgtgca gtgcatcggc tttttcagga acggggtggg
     7561 gcggccctgc cgcatgaact ccgcgccaag gattttttca tcaaacgcga tgttgtgcgc
     7621 aatggcgaag gtcgcctgct cgatcgccgg cagcacctcg tccagcacct ccagcagcgg
     7681 ccggccctgc ttcatggcca tttcggtcgt gatgccatga acccgcgccg catccggcgg
     7741 caccacaaaa ccctctggcc ggatgatggt tgtgcaagcg ctcagctcgt tgccgtcgtc
     7801 atcacacaaa atccatgcca actggatcat ccgcggccag ttgttcaacg tccgcgggtg
     7861 cagttgcgcg ttccaatcgc gcggcagccc ggtggtttca gtatcgaaaa aaaacaaccg
     7921 ccggttcgac tgcccacgaa atggaagatt catcgtcatg gctcgcatcc taccccaact
     7981 ttacccccca cgcaacctcc ccacggttcc gattcaaacg gggtggggcg ccggcccgca
     8041 ttttcttttc ctttgcctga accaccttcc tctctatatt caccacaaat ggatttgcca
     8101 gcgctccagc ttagtgtggt catcgtcaac tggaacacgc gggaactcct ccgcgcatgc
     8161 ctcgaatccg tccgccagca caccggcgga atcacctacg aaattatcgt tgtggataac
     8221 gcctctcacg atggtagccc cgccatggtg cgccagcagt ttcctgatgt gcggctgatt
     8281 gaaaacaccg agaacctcgg cttcgccgcc gccaacaatc aggccatccg gatggcccgg
     8341 ggcaatgccg tcatcctgct caacagcgat actgagctcg tcgaaaactc cctccgcatc
     8401 ctccacgatt tcctcttctc tcgagactcc atcggcgccg ttggcgggcg gctcatctac
     8461 cccggcggcg ccccccagtg gtcctatggt ttcgccccct ctctcggccg catgctctgg
     8521 atcaccctct ccggcttttt gcgcatccaa cgcggacgca aacccggcgc ggtgattccc
     8581 caagccgatg agcccgccca ccccgtcgaa tatattgtcg gcgc
//
LOCUS       DAOPNP010000105         8604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_9178_length_8604_cov_9.011112, whole genome shotgun sequence.
ACCESSION   DAOPNP010000105 DAOPNP010000000
VERSION     DAOPNP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9178_length_8604_cov_9.011112"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1183)
                     /locus_tag="PKI64_08925"
     CDS             complement(116..1183)
                     /locus_tag="PKI64_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE01167.1"
                     /translation="MNLTAVHQLVAGFAAGDAISLEAVAIREACRALGYASDIFAPAE
                     SVTPDTRHDVRPLEELPSVATQALIYHYSIQSRATDVFRRAAARKVMIYHNITPAEYF
                     RGFDEAVERQLTLGRQELGSIAALADAVWADSAFNAQELAALGVQNVRVLPLLFSPTM
                     LEVAPDSAIQQRLATPSKKILFVGRIAPNKCVEDLIEVFAWYHRHLERRSELLLIGSE
                     RSCPRYFAMLRMYAGELDLPAVSFVRYASPAGLVAFYEGADLFVTTSRHEGYCLPVVE
                     AMYKSVPVLARNVGGVPEAMDGAGVMFDDASPMELACLMHRLITPGPLRSTVLAAQQA
                     RIERLRARPVLQELRELLAAL"
     gene            complement(1180..1626)
                     /locus_tag="PKI64_08930"
     CDS             complement(1180..1626)
                     /locus_tag="PKI64_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01168.1"
                     /translation="MKLSVAGQDQSASLGNEIAAEAARLAQVPGGIGALDPAHSARQL
                     AAATSEADYLQHYLKLLRMRQGVRTSDFYIPRRAGWVGHFVACIKARLWRLLRYQHDR
                     LTLQQNLINELAAHAVEFQQEAWQQEVADLKRRVAALEQKNGVGSA"
     gene            complement(1634..2665)
                     /locus_tag="PKI64_08935"
     CDS             complement(1634..2665)
                     /locus_tag="PKI64_08935"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE01169.1"
                     /translation="MNTTLLDGLWQEGRDFLGVRYPILCGAMTWISDATLVTAVSKQG
                     GFASLAAGNMPGEALAAEIAKTAAQVNGSFAVNLVTIAPNFPAHKEAVLQSSVPIVIF
                     AGNFPKKHDVAQMKEAGKKVLSFASTDSIADQMVRFGVDGLILEGSEAGGHIGHVSTM
                     ILLQQVLFHKPNVPVFVGGGIATGRMLAHMILMGAAGVQMGTRFVMSTECNAHPKLKE
                     RFVRARAREAMSTPEYDKRLPIVAVRALKNKSTEAFGRLQLELLQLMNEGKISHMEAQ
                     YRVEGFWMGGLRKAAVEGDVDYGSCMAGQSVGLVDKIQPMSEIFAELLQEGEAELASI
                     QARLAGIKR"
     gene            complement(2794..3312)
                     /gene="idi"
                     /locus_tag="PKI64_08940"
     CDS             complement(2794..3312)
                     /gene="idi"
                     /locus_tag="PKI64_08940"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOE01170.1"
                     /translation="MAAEFVILVDEQDRPVGRMEKLAAHEHGGTRHRAFSVFLVDAQG
                     RWLLQRRAAGKYHFPNLWTNACCSHPRPGEETEAAAHRRLREELGVACALTHRFRFEY
                     KATSEAEGLTEHEMDHVFTGVYTGELQPNPEEISEIRWVSRADLEQEVAAHPERFTPW
                     FKLAMVRVREWL"
     gene            complement(3322..4368)
                     /locus_tag="PKI64_08945"
     CDS             complement(3322..4368)
                     /locus_tag="PKI64_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOE01171.1"
                     /translation="MKKAGATTTMATVPMQHVGPIQLAGPELSGEFMVPLATYESPLW
                     PSVNRGARVTAQAGGIQAVIFDERMTRSVLVVAPDVVAAQNVVEFVQQSRAELAKVAA
                     GTSRFAKLVDIHFEVVGHLVFIRFEFTTGDAAGHNMATRAADALLAWILERQPNCQYG
                     SISGNYCCDKKPSAVNGILGRGKYTVAEVTLSARLVARYLHSTAEKIAELNTRKNLVG
                     TALAGGLRSANAHFANMLLGFYLATGQDAANIIEGSQGFTLAEARPAGALYFSVTLPN
                     LIVGSVGNGKELPFVQDNLRALGCLEKRPAGQNARRLALLCAATVLCGELSLLAAQTN
                     PGELMAAHLRLERK"
     gene            complement(4400..5743)
                     /locus_tag="PKI64_08950"
     CDS             complement(4400..5743)
                     /locus_tag="PKI64_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01172.1"
                     /translation="MKHGAPTLWPRGWACGMLGLALGLAGCGQVTPQKEYEAGVAALH
                     VGRLTQGKELLESALERDPAGTFAATAQNWLGLVNWELGQTAEAMSNFEGAAKLNPAA
                     FDPVYNLGVLALELGDPQRGIALLRRAADLDARDIRALLAIGEWTTRNGRWDLAKRMY
                     FEVLRREPQTAAAATGLGRVALLEGQQAQAETFFMQALEMSKDYPPALYNLGVLYAQS
                     SGHGEQAGEYFRQYLKVAAQGPRAAAASARIGGQAIEQDSFKVHAPAPQPKSAAAIMW
                     AQARETLANGDKEEAYIQMLRALETAREGGDMSQTGEILKRALEVFGDRAMVQLEAGE
                     YQLRLNHPAEAQAAMLKAQALEPDNPMVLFVLARTSLAAEEYDTAVISLRKLVQLEPG
                     NADALWTLADVYGDKLGMISKGTAVFRDFERLFPSDPRVGEISARIKALEEAAAH"
     gene            complement(5740..6141)
                     /locus_tag="PKI64_08955"
     CDS             complement(5740..6141)
                     /locus_tag="PKI64_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE01173.1"
                     /translation="MVKISITTSRRTELVPVSRPIQAALNTLPAWDGLLNIHSPHTTA
                     GVTVNEQADPDVAADMEQALAKLVPWHANYAHAEGNSAAHIQTALIGTSVMVPVQKGR
                     LALGTWQGIFLCEFDGPRTRELWLTPIKEAT"
     gene            6229..7467
                     /locus_tag="PKI64_08960"
     CDS             6229..7467
                     /locus_tag="PKI64_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE01174.1"
                     /translation="MTLLLVGAFLGLLACSAFFSSAETAYFSIDPIQLRRMSKQRPAT
                     GERLRAILANPTRLLSTLLIGNTLVNVAIANVGYLLATRWFPAYAEAAAITAVTILLI
                     FFGEVGPKNIGLLHTAVLVRWYGPLIQVCEVVLAPLRRMLDLISQRLATLFRPHGKTL
                     SEEEFETVLDISREEGILNADELAMIKAIVDLEDLRAADVMTPRVDFIGLDLDDPDVE
                     PLAAVRKARRNFLILYHGQFDDIAGFLDVRKYLLDPAHSIAAATLPPVFVPESIQLNR
                     LLVRFQKDKIRIAIVVDEYGGVAGLVTRGDILEEITGDIYNELSKPRPIFQSAGPHAW
                     LVDANISLEELNRKLRLNLEADNADRLAGWITEHLGNVPKQDDVVEVQGVRVRVMQTI
                     RLRVALAHIEKTDVAHEVQP"
     gene            7464..8432
                     /locus_tag="PKI64_08965"
     CDS             7464..8432
                     /locus_tag="PKI64_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOE01175.1"
                     /translation="MTSLLIFLLLGCLLGSAFFAGIETGVIAMNRLRLQHLVRRQVRG
                     AKIIRHFLTHPDLLLGTTLIGTNLLHVVSAVIAAALGRQLGGTIGTTVAGVLLVLVTL
                     VFCEYIPKAWFQAAPARRTLPFARLLRLAAIVLRPASYLVNSAIRVILPRRAGKNVGG
                     KLLVTREELLHLTREGVQSGVLTRHESQMIHGVFELTHKTCAQIMTPRDKMVVVPATA
                     TPEEVLAIARQREFNRLPVFDSAQKTFVGVVHIFDILADDAPASKTAAHYMRPPQLVA
                     SYLPVDHLLPRMRVTKLPLFLVTDDRYEVIGLITLEDVLQEVTGED"
BASE COUNT         1628 a         2828 c         2477 g         1671 t
ORIGIN      
        1 gttgatccac atcaccatga tgcatgaacg aaaccccata gcatctacaa tccaagcggg
       61 atgcggccat gactgccttg catctgaggc agacttgtcg cacgcgttat tcaggtcaaa
      121 gcgcggccaa cagttcgcgc agttcctgca gtactggccg cgcgcgcaag cgttcaatgc
      181 gggcctgctg cgcggccagc acggttgacc gcaaagggcc gggcgtgatt agccggtgca
      241 tcaggcacgc caattccatc gggctggcat catcaaacat gacccccgcc ccatccatgg
      301 cttccggcac accgccgacg ttgcgggcca gcaccgggac gctcttatac atggcttcca
      361 ccaccggcag gcagtagcct tcatggcggc tcgtggtgac aaacaaatcc gcgccttcgt
      421 aaaaagccac cagccctgcc ggcgaggcgt aacgaacaaa actgaccgcc ggcaggtcca
      481 attcacccgc gtacatgcgc agcattgcga aataccgcgg gcaggaccgt tcggagccaa
      541 tcaacagcaa ttcgctgcgg cgttcaagat gccggtgata ccaggcaaac acctcgatca
      601 aatcttccac gcatttgttg ggcgcaatgc gcccgacaaa gagaatcttc ttggaaggtg
      661 ttgcgagtcg ttgctgtatt gccgagtcgg gagcaacctc cagcatggtg ggtgaaaaga
      721 gtagtggcag cacacgcaca ttctggactc ccaaggcggc aagttcttgc gcgttgaagg
      781 cggaatcggc ccaaacggca tcggccaaag cggcgatgct ccccaactcc tgccggccca
      841 aggtcagttg ccgttccacg gcttcatcga atccgcggaa atattccgcc ggagtgatgt
      901 tatgataaat catcactttg cgcgcggccg cccgccgaaa aacgtccgtg gcccggcttt
      961 ggatggaata atggtaaatc agcgcctgcg tggccacgga cggcaactcc tccagcgggc
     1021 gcacgtcatg acgggtgtcg ggcgtgacgg attccgccgg cgcaaaaata tcgctggcat
     1081 agcccaaggc gcggcacgcc tcacgaatgg ccaccgcttc cagcgaaatg gcatcgccgg
     1141 cggcaaaccc ggccacaagt tgatgaacgg ccgtaagatt catgccgacc ccaccccatt
     1201 tttttgctcc aatgccgcca cacggcgttt gaggtccgcc acttcctgct gccacgcctc
     1261 ctgctgaaat tccaccgcat gggcggccag ctcgttgatg aggttttgct ggagggtcag
     1321 ccggtcatgc tggtagcgca gcaaccgcca cagccgtgct ttgatacaag caacgaagtg
     1381 cccgacccac ccggcgcggc gcggaatata aaaatcactg gtgcgcacgc cctggcgcat
     1441 tcgcagcaac tttaaatagt gctgcaaata atccgcctct gatgtagccg cggccaattg
     1501 ccgggcagaa tgtgcggggt ccagggcacc gatgccccca ggaacttgcg ccagccgcgc
     1561 ggcctcggca gcaatctcgt tcccgaggct ggcgctttga tcctggccgg cgaccgagag
     1621 cttcatggcc gcgctagcgt ttgattccag ccaggcgcgc ctggatggag gccagttccg
     1681 cttcgccctc ttgcagcagc tcggcaaaga tttcgctcat cggctggatt ttatccacta
     1741 gcccgacact ctggccggcc atgcaggacc cgtaatccac gtcgccctcc acggccgcct
     1801 tgcgcagccc acccatccag aaaccttcca cgcggtattg cgcttccatg tggctgatct
     1861 tgccttcgtt catcagttgc agcaattcca gttgcaggcg gccgaaagct tcggtggatt
     1921 tattcttcaa cgcgcgcact gccacgatcg gcagacgctt gtcgtattcc ggcgtgctca
     1981 tggcctcgcg ggcccgggcg cgcacgaagc gttccttgag cttgggatgc gcgttgcact
     2041 ccgtgctcat cacaaagcgg gtgcccattt gcacgccggc cgcgcccatc aaaatcatgt
     2101 gtgccagcat ccggcctgtg gcaatgccgc caccaacaaa taccggcaca ttgggcttat
     2161 ggaacaacac ctgctgcagc aagatcatcg tggaaacatg gccaatgtgg ccgccggcct
     2221 cactgccttc cagaatcagg ccatccaccc cgaagcgcac catctgatcg gcaatggaat
     2281 cggtggaggc aaaggacagc actttcttgc cggcctcctt catttgcgcc acatcgtgct
     2341 tcttcgggaa gttgccggca aagatcacga tcggcacgga tgattgcagc accgcctctt
     2401 tatgcgccgg gaagttgggg gcaatggtca ccaaattgac ggcaaacgag ccgttgacct
     2461 gtgctgcggt tttggcgatt tccgcggcca aggcttcgcc gggcatgttg ccggcggcca
     2521 aggaagcaaa gccaccctgc ttgctaaccg cagtgaccag cgtcgcatcg ctgatccacg
     2581 tcatggcgcc gcacaaaatg gggtagcgta ctccgagaaa atcgcgccct tcctgccaca
     2641 aaccgtccaa aagtgttgta ttcattctga ttactccttt ggtcacaccc ggcaacacta
     2701 ccccttcagg gccggaaaat catctggaaa aatcgctttt tatgcacttt aggtgcgccg
     2761 ttgtgttcgt cacgacgacc ggcgagcagc ttttcaaagc cattcgcgca cgcgcaccat
     2821 cgccaatttg aaccacgggg tgaagcgctc cggatgtgct gccacttcct gctccaaatc
     2881 cgcgcgggag acccagcgaa tttcgctgat ctcctccgga ttgggctgga gttcgcccgt
     2941 atacactccc gtgaaaacgt gatccatttc gtgttccgtc aacccttcgg cctcggaggt
     3001 ggccttatac tcgaaacgga accgatgggt cagggcgcag gccacgccca gctcttcgcg
     3061 caaccggcgg tgcgcggcgg cctcggtttc ctcaccgggg cgcggatggc tgcagcaggc
     3121 gttggtccac aaattgggga aatgatattt gccggcggcg cgccgttgca ggagccaacg
     3181 cccttgcgca tccaccaaaa aaacggaaaa cgcgcgatgg cgcgtgccgc catgctcgtg
     3241 ggcggccaac ttttccatgc ggccaacggg ccgatcctgc tcatccacca aaatgacgaa
     3301 ttctgcggcc atggctcgcg cttacttgcg ctccaaccgc aagtgcgcag ccatcaactc
     3361 gcccggattg gtttgcgcgg ccagcagcga aagttcgccg cacagcaccg tggccgcgca
     3421 gagcaatgcc agccgccgcg cattttgtcc ggcgggtcgt ttctccaaac agcccaaagc
     3481 gcgcaggttg tcctggacaa acggcagttc tttgccgttg cccaccgacc cgacaatcag
     3541 gttggggagc gtcacggaaa aatagagcgc gccggccggg cgggcctccg ccagcgtgaa
     3601 cccctgcgac ccttcgatga tgttcgccgc gtcctgcccc gtggccagat aaaagcccag
     3661 cagcatgttg gcaaaatggg cattggcgct gcgcaggccg ccggccagcg cggtgccaac
     3721 caggtttttc cgcgtattca actcggcaat tttttccgcg gtgctgtgca gataacgcgc
     3781 gaccaggcgc gcggataacg tcacttccgc cacggtgtat ttgccccggc ccaaaatgcc
     3841 gttcacggcc gacggctttt tatcgcaaca atagttgccg gagatggagc catattggca
     3901 gtttggttgc cgctccagaa tccaagccag cagggcatcg gccgcgcgtg tcgccatatt
     3961 gtggccggcg gcatcgccgg tggtaaactc aaagcgaatg aacaccagat gcccgaccac
     4021 ctcaaaatga atatccacca gcttcgcaaa acggctggtg ccggcggcca ctttggccag
     4081 ttccgcacgc gattgctgca caaactccac cacgttttgg gcggccacca catccggggc
     4141 caccaccagc accgagcggg tcatgcgttc atcaaaaatg accgcctgaa tgccgccggc
     4201 ttgcgccgtg acgcgcgccc cacggttgac cgaaggccac agcggcgatt catacgttgc
     4261 cagcggcacc atgaattcgc cgctcaattc cgggccggcc aattgaatcg gccccacgtg
     4321 ctgcatgggg accgtggcca ttgtggtggt tgcgcctgct ttcttcatgc cgctactccc
     4381 tgcctgccgt cggccgcgct caatgtgccg cggcttcctc caaggccttg attcgcgcgg
     4441 agatctcccc cacccgcggg tcggatggga acaaccgctc aaagtcgcgg aagaccgccg
     4501 tgcccttgct gatcatgccg agtttgtcgc catacacatc ggccagtgtc cagagcgcat
     4561 cggcattgcc cggctccaac tgcacgagct tgcgcagcga gataaccgcc gtgtcatatt
     4621 cctccgcggc cagcgaagtc cgcgccagca caaagagtac catcggatta tccggctcca
     4681 gcgcctgtgc cttcagcatg gcggcttggg cttctgccgg atggttcagg cgcagttgat
     4741 actcgcccgc ctcaagctgc accatggcgc ggtcgccgaa cacctccagc gcgcgcttga
     4801 ggatttcgcc ggtttggctc atatcaccgc cctcgcgcgc ggtttccaat gcacgcaaca
     4861 tttggatgta ggcttcttcc ttgtcgccgt tggccagtgt ttcgcgggct tgtgcccaca
     4921 tgatggccgc cgccgatttg ggctgcggcg cgggcgcgtg caccttgaaa gaatcctgct
     4981 cgatggcctg cccgccgatg cgcgccgacg ccgccgccgc gcgcggtccc tgtgcggcca
     5041 ctttcaggta ttgccggaag tactcgcctg cctgctcgcc atggcctgat gactgggcat
     5101 agagcacgcc aaggttatac agggccggtg ggtagtcctt gctcatttcc agcgcctgca
     5161 tgaaaaatgt ttccgcctgc gcctgctggc cttccaacaa ggcaactcgt cccaggccgg
     5221 tggccgctgc cgcggtttgc ggctcgcgcc gcagcacttc aaaatacatc cgcttggcca
     5281 aatcccagcg tccattgcgc gtggtccatt cgccgatggc cagcagcgcg cgaatatccc
     5341 gcgcatccag gtcggccgcc cggcgcaaca gcgcgatgcc gcgttgcgga tcacccagct
     5401 ccagcgccag cacacccaga ttatacaccg gatcaaatgc cgccgggttc agcttggccg
     5461 cgccttcaaa gttgctcatg gcttcggccg tctgccccag ctcccagttg accaggccca
     5521 gccagttctg tgcggtggcg gcgaaggttc ccgccgggtc gcgttccagc gcggactcca
     5581 gcaactcctt gccctgcgtc aggcggccga catgcaacgc agccacgccg gcttcatatt
     5641 ccttttgcgg cgtcacttgg ccgcagccgg ccagccccaa ggccaagccc agcattccgc
     5701 aggcccagcc gcgcggccaa agtgtgggcg cgccatgctt catgtggcct ccttaatcgg
     5761 ggtcagccac aactcccgcg tgcgcgggcc atcaaactcg caaagaaaaa tcccctgcca
     5821 cgtacccagc gccaaccgcc ctttttgcac gggaaccatc acggatgtgc caatcagtgc
     5881 ggtctggata tgcgcggcgg agttgccttc ggcatgggca taattggcgt gccaaggcac
     5941 cagcttggcc agcgcctgct ccatatcggc ggccacgtcg gggtccgcct gctcattgac
     6001 ggtcacaccg gcggtggtgt gcggcgaatg gatattcagc agcccgtccc acgccggcag
     6061 cgtgttcagc gcggcctgaa tggggcgcga caccggcacc aattcggtgc ggcgggaagt
     6121 tgtgatggat attttcacca tggccccgta ctatacgcat agtttcggat tgcggaaagt
     6181 gaaacctgca aacacatctt gcgcccggcg cctgcggaac tcgcacccat gacgttgctt
     6241 ctggttggcg cctttttggg cttgttggcg tgctctgcct ttttttccag tgcggaaacg
     6301 gcctattttt cgattgatcc gatccagttg cgccggatga gcaagcagcg cccggccacg
     6361 ggcgagcggc tgcgcgccat tttggccaat cccacgcgct tgctctccac cctgctgatc
     6421 ggcaacacac tggtcaacgt ggccatcgcc aacgtgggct atttgctggc tacccgctgg
     6481 tttccagctt atgccgaggc ggcggccatc accgcggtca ccatcctgct gatctttttt
     6541 ggcgaggtgg ggcccaagaa catcgggttg ctccacaccg ccgtgctggt gcgctggtat
     6601 gggccgctga tccaagtgtg cgaagtggtc ctggcccccc tgcgccggat gctggacctg
     6661 atcagccagc gcctcgccac gctcttccgg ccgcacggca aaacactctc cgaggaagag
     6721 tttgagaccg tcctcgatat ttcccgggag gaaggcatcc tcaacgccga tgagctggcc
     6781 atgatcaagg ccattgtgga cctggaagac ctgcgcgccg ccgatgtcat gaccccgcgc
     6841 gtggatttta tcggcctgga tttggatgat cccgacgtgg aaccactcgc ggccgtgcgc
     6901 aaggcccgcc ggaacttcct gattctctac cacgggcagt ttgacgacat cgccggtttc
     6961 ctcgatgtgc gcaaatacct gcttgatccc gcccattcca tcgccgcggc gacgctgccg
     7021 ccggttttcg tgccggaaag catccagctc aaccggctgc tggtccgttt tcagaaggat
     7081 aaaatccgta ttgcgatcgt ggtggatgaa tatggcggcg tggccggttt ggtcacccgc
     7141 ggcgacattc tcgaagaaat cactggcgat atctacaacg agctgtccaa gccccgcccg
     7201 attttccaat cggccggccc gcacgcgtgg ctggtggatg ccaacatttc gctggaagaa
     7261 ctcaaccgca agctgcggct gaatctggaa gccgacaatg ccgaccgcct ggccggctgg
     7321 atcaccgagc atctgggcaa cgtgcccaaa caggatgacg tggtggaagt gcagggcgtg
     7381 cgggtgcgcg tcatgcagac catccggctg cgcgtggccc ttgcgcacat cgaaaaaacg
     7441 gatgtggccc acgaggtgca gccatgacct cgctgctgat tttcctcttg ctcggctgcc
     7501 tgttgggaag cgcctttttt gcgggcattg aaaccggcgt cattgccatg aaccggctgc
     7561 gcttgcagca tctggtgcgc cgccaggttc gcggcgccaa aatcattcgc catttcctga
     7621 cccaccccga tttgctcctg ggcaccacgc tcatcggcac caacctgctg cacgtagtca
     7681 gcgcggtgat cgccgcggcg ctgggccgcc aactgggcgg caccatcggc accacggtgg
     7741 caggcgtact gctggtgctg gtgacgctgg tgttctgcga atacatcccg aaagcctggt
     7801 tccaggccgc cccagcgcgc cgcaccctgc cctttgcgcg actgctgcgc ttggcggcca
     7861 tcgtcctgcg cccggccagc tatctggtca acagcgccat ccgcgtgatt ttaccgcgcc
     7921 gcgcgggcaa aaacgtgggc ggcaaactgc tcgtcacccg cgaagaactg ctgcacctga
     7981 cgcgcgaggg cgtacaatcc ggcgtactca cccgccatga atcgcaaatg atccacggcg
     8041 tcttcgagct gacccacaaa acctgcgcgc aaatcatgac cccgcgcgac aaaatggtgg
     8101 tggtgcctgc caccgccacg ccggaagaag tgctggccat tgcccgccag cgggagttca
     8161 accgcctgcc ggtgtttgac tccgcgcaaa aaacattcgt cggcgtggtg cacatcttcg
     8221 acattttggc ggacgacgcc cccgccagca aaaccgcggc gcattacatg cgcccgccgc
     8281 aactggtcgc gtcctatctg ccggtggacc atttgctgcc gcggatgcgc gtcaccaagc
     8341 tcccgctgtt cctggtgacg gatgatcgct acgaagtgat cggcctgatc acgctcgaag
     8401 acgtgctgca ggaagtcacc ggcgaagact gacgccaacg ggcctctaca cccggaacac
     8461 tgcgccgagt ttcttgcctc tgagttccgt attgagggga ccaacaattt cgctgatttt
     8521 gccgaccttt tctagggggg aatgattgag ggatgaaacc ttcccaccag gaacggctgg
     8581 aaggtctgcg acctttccag ccct
//
LOCUS       DAOPNP010000106         8598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_9185_length_8598_cov_7.709704, whole genome shotgun sequence.
ACCESSION   DAOPNP010000106 DAOPNP010000000
VERSION     DAOPNP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9185_length_8598_cov_7.709704"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(509..1579)
                     /locus_tag="PKI64_08970"
     CDS             complement(509..1579)
                     /locus_tag="PKI64_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOE01176.1"
                     /translation="MSVHADQSMWSRHEREFRRRVYPAATAADWQDWRWQLRNRARTA
                     AAVRRALGAVSPREARGLAAVRAWRMPLGITPYLLALLATEAPDGPLRRTLLPDEREG
                     IRAPGELLDPLGEEAHQVAPGLIRSYPHKALLLATTDCACFCRFCTRARTAGRPAARW
                     AAALAWLRRTPEIRDVLISGGDPLTLDDAALGRLLGELRAIPHVELLRIGTKIPAALP
                     QRVTPALARLLKAHRPLWLSLHFTHPAELTPRTAAACRRLLDAGIPLMSQTVLLRGIN
                     DDLPTLCRLNEGLLTLGVKPYYLHQGDLAAGTAHLRSTVARGRRILRDLCGLTTGYAV
                     PHFMLDRPGGGGKVPLGPAWPF"
     gene            1627..2841
                     /locus_tag="PKI64_08975"
     CDS             1627..2841
                     /locus_tag="PKI64_08975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE01177.1"
                     /translation="MPNDPVQFDQTTAIDVTPGVTPDDTTVTDTQAPTDASILRDLAP
                     TRVPLGTGSRQLTANYRAILAARAIYHPIAYRFPRELGRGRQGIVYLGLRQGARGCVT
                     RHAIKLFDPSIYGSAKKYWTDMARIAAQTSKLQLVKNPALVAPDIYEESNGIGYIQME
                     VVDGISLRELLDGTHLEQVRARSTDAEWRRFTDVIFRQEGGKLMIQPGIALYILRQVL
                     RGLETLHEMGFIHSDVKPANVMINRLGYVKLIDYGRAMLVDEKMTLLLGTPVYMAPEV
                     HTERVARTRSDIYSLGLVGLEMLRGERLFSPEVARSESELLKAKNNLPGRLYDILPEH
                     VRQNAQFLILLQRFLEPNPAGRYASAEEAEIGEQGLSMLHKQLTLAGKDTEYGRELEN
                     YLSKIVNPLGPT"
     gene            3029..3622
                     /locus_tag="PKI64_08980"
     CDS             3029..3622
                     /locus_tag="PKI64_08980"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOE01178.1"
                     /translation="MNLLAHLHLSTGQPVAMAAGNLLADFMRRFGATDIDPEFAAGVR
                     LHRAIDVFTDSHPLIRAARRLIAPPRNRLAGIIIDIALDHQLSRHWNAHAALPLTTFV
                     RARLAEIQAYLAAHDTPLNWVIRRAIRDDWLLSYATLAGLDLTFERVASRAPVAAALR
                     GATADIARHDAHFQQLFHAFYPALRAHAAQTHAALAV"
     gene            3767..3958
                     /locus_tag="PKI64_08985"
     CDS             3767..3958
                     /locus_tag="PKI64_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOE01179.1"
                     /translation="MRTTIIIKEEILSKASALTGVKEKTALVHMGLEALIARASAARL
                     ARLGRSERGLRPIPRRRVP"
     gene            3959..4312
                     /locus_tag="PKI64_08990"
     CDS             3959..4312
                     /locus_tag="PKI64_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE01180.1"
                     /translation="MMLVDTSVWVDHLRNGNDALADLLLRDEVACHPMVIGELACGNL
                     KCRKEILALLQALPTVECVSDDEILFFIGQHRLSGLGLGLVDVHLLASCAIAHTSLWT
                     LDSRLQRAADELSNK"
     gene            4440..4797
                     /locus_tag="PKI64_08995"
                     /pseudo
     CDS             4440..4797
                     /locus_tag="PKI64_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4794..5141
                     /locus_tag="PKI64_09000"
     CDS             4794..5141
                     /locus_tag="PKI64_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015193651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOE01181.1"
                     /translation="MSRPFKKLLENMPRDRRERIRLKTEVLKNEMALKDLRQALKLTQ
                     DELARSLKMKQAAISKFERQSDIYLSTLRKILFVMGADLKIVARFPEGDVLINQFNDI
                     TSANTPEDIAPVS"
     gene            5164..5616
                     /locus_tag="PKI64_09005"
     CDS             5164..5616
                     /locus_tag="PKI64_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01182.1"
                     /translation="MKEKKMKSPTKKKADKPRCGLCGKTKNLIKTECCGNWICDDEHK
                     YVMFSYARNSCSRNHRRYTLCGYHYNEGHKGDWKTCKKCRSDFEPEMVVWYGTNEYNF
                     EKMPDPPSFTPTKCSKCGQVIVLGNGGYAHGPDGYTCGECFAKDNRRK"
     gene            5677..5838
                     /locus_tag="PKI64_09010"
     CDS             5677..5838
                     /locus_tag="PKI64_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01183.1"
                     /translation="MVEKTVCRCRDAAALRGATADIATHDAHFQQLFHAFYPALRAHT
                     AQTHAARAV"
     gene            6025..6717
                     /locus_tag="PKI64_09015"
     CDS             6025..6717
                     /locus_tag="PKI64_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE01184.1"
                     /translation="MTDQATIQQPKPAGDRRLMRWILVLQLVILGWLAKNYFDGAFNR
                     RHHNPPAVATQPDTPPSLEPSAQPNAPSPFSVLPRWTAFQRATPPPRLVHPMDRIRLE
                     MERMMAEANRAAANFDALFGLDDSLWSALPASPAMNLRELPDAYELSLALPNTDPGSV
                     DVQLDGRLLSVTAQQNTQTAHSTSAQRFSSRVLLPGPVAANAACQVTNENDRLCIRIP
                     KATAPQTAARQP"
     gene            6744..7737
                     /locus_tag="PKI64_09020"
                     /pseudo
     CDS             6744..7737
                     /locus_tag="PKI64_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            7875..>8598
                     /locus_tag="PKI64_09025"
     CDS             7875..>8598
                     /locus_tag="PKI64_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOE01185.1"
                     /translation="MEFQEVREYVPGDDIRSIDWNVTARTGNPHVKKFTEERELTVML
                     LVDISASNLFGSTAQLKRDLAAELAAVLAFSAITNHDRVGLILFSDRVEKHIPPAKGP
                     THVLRVISEVLNARPVSPRTDLAPALRYLNQVVRRRCVAFLISDFIAPDCDRLLRTTA
                     RRHDLVSLIIGDKHEHALPAVGLVDFQDAETGRRLWLDTSHAPTRRALLTRQAERRTR
                     LLAQLARANSDAIELHAGERYDR"
BASE COUNT         1821 a         2946 c         2306 g         1525 t
ORIGIN      
        1 ggtgaccgcg gggatttttc cggacaccgg cctcaccgag gccggctaca ataagacagg
       61 atatccaaca gcccgctgcc ggtacaacgg cggcctacag gcctcgcccg ctgtgcgcag
      121 cacatggctg ctccctgaac tctgaaccct gacctctgac ctctccgccg tccgccgtgc
      181 gcagcacatg gctgctccct gaactctgaa ccctgaactc tgacctctct gcagtccgct
      241 gtgcgcagca cctgcatgcg gaggatttct tgcggatttt ggcgaggttg ctacgcaaaa
      301 ataagcggcg gattttctga tgatggcgcg ttgcggacga cccgagctgg actcgtgtcc
      361 gcgagtggtc ggacgcggcg cgccagggcg gaaaagacgc cggttggttt ttgcgccgcc
      421 aaaatttgca agaaatcctc ctaatccaac acagaagcac gcaacacaaa acccgctcgc
      481 tcgccaagac gccaagaacg cgaacaggtc agaatggcca cgcggggccc agaggcactt
      541 tgccgccgcc acccggacgg tcgagcatga agtgcggcac ggcgtaaccg gtggttaggc
      601 cgcacaagtc gcgcaggatg cggcgcccgc gcgccacggt ggagcgcaaa tgcgcggtgc
      661 ccgccgccag atcgccttga tgcaggtaat agggcttcac gcccagcgtc agcaggcctt
      721 cgttcagccg gcacagcgtg gggaggtcat cgttaatacc gcgcagcagc acggtttggc
      781 tcatcagcgg aatgccggca tccagcaggc ggcggcacgc cgccgccgtg cgtggcgtca
      841 attccgccgg atgcgtaaaa tgcaggctaa gccacaaagg ccggtgcgcc ttcaacagcc
      901 gtgccaaggc cggcgtgacg cgctgcggca gcgccgccgg aattttggtg ccaatgcgca
      961 acagctccac atggggaatc gcgcgcagct cgccaagcag gcggccgagc gcggcatcgt
     1021 ccagcgtcag cgggtcgccg ccggaaatca ggacatcgcg aatctccggc gttcggcgca
     1081 gccacgccag cgcggcggcc cagcgcgcgg ccggccggcc cgccgtgcgc gcccgcgtgc
     1141 aaaaccggca gaagcacgcg cagtccgtcg tggccagcaa cagcgccttg tggggatagg
     1201 aacggatcaa ccccggcgcg acttgatgcg cctcctcgcc cagcggatcc agcaactcgc
     1261 cgggggcacg gattccctcg cgttcgtcgg gcaggagcgt acgccgcagc gggccatccg
     1321 gcgcttccgt cgccaacagc gccagcaaat acggcgtgat gcccagcggc atgcgccacg
     1381 cccgcaccgc ggccagtccg cgggcctccc gcggcgacac cgcgcccagc gcgcggcgca
     1441 ccgctgccgc cgtacgcgcg cgattgcgca actgccagcg ccaatcctgc caatccgccg
     1501 ccgtcgccgc cggataaacc cggcggcgga actcccgctc atggcgggac cacatggact
     1561 ggtcggcgtg gacgctcatc ggcaaccgat attgccaaag cgccgccttt aggtaaactc
     1621 tcccgcgtgc ccaacgatcc cgtccaattt gatcagacta ccgcgattga cgtcaccccc
     1681 ggcgtcactc cggacgatac caccgtcacc gatacccagg cgcccacgga tgcctccatc
     1741 ctgcgcgacc tcgcccccac ccgcgtgccg ctcggtaccg gcagccggca gctaaccgcc
     1801 aactaccgcg cgattctggc cgcgcgcgcc atttatcatc ccattgccta ccgcttcccg
     1861 cgcgagctgg gccgcgggcg gcagggcatc gtctatctgg gcttgcggca gggcgcccgc
     1921 ggctgcgtca cccgccacgc catcaagctg ttcgatccct ccatctacgg cagcgcaaaa
     1981 aaatactgga ccgacatggc gcgcatcgcc gcgcaaacgt ccaagctgca actggtgaag
     2041 aacccggcgc tcgtcgcgcc ggacatctac gaagaaagca acggcatcgg ctacatccag
     2101 atggaagtgg tggatggcat ctccctgcgt gagctgctcg atggcaccca cctcgaacag
     2161 gtgcgcgcgc gctccaccga cgccgagtgg cgccgcttca ccgatgttat cttccggcag
     2221 gagggcggca agctgatgat ccagccgggc atcgcgctct acatcctgcg ccaagtcctg
     2281 cgcggcctgg aaaccctcca cgaaatgggc ttcatccatt ccgacgtcaa acccgccaac
     2341 gtcatgatca accgcctcgg ctacgtcaaa ctgattgact atggtcgcgc gatgctcgtg
     2401 gatgaaaaaa tgacgctgct gctcggcacc cccgtttaca tggccccgga agtccacacc
     2461 gagcgcgtcg cgcgcacgcg ctccgatatc tacagcctcg gcctcgtagg gctggaaatg
     2521 ctccgcggcg agcggctgtt ctcgccggaa gtggcccgtt cggaatccga actgctcaag
     2581 gccaagaaca acctccccgg ccgcctctac gacatcctcc ccgaacacgt ccgccaaaac
     2641 gcgcagttcc tcattttgct ccagcgtttt ctggaaccca atcccgccgg ccgctacgcc
     2701 tccgccgagg aagccgaaat cggcgaacaa ggcctctcca tgctgcacaa gcaactcacc
     2761 ttggctggca aggataccga atatggccgc gagctggaaa actacctctc caaaatcgtc
     2821 aaccccctcg gccccacctg accccctgcc ccccccgaag ttacacaaac gcgagccatg
     2881 accgatccaa acgctaaggc agacaacgga aagaagcaaa ggcgccccaa tcacattaat
     2941 gtggtactaa atgtggtact aactccgccg cgcaaggttt tgagaaatta gcccccacct
     3001 gaccccctgc ccacccccgc cctgaaccat gaacctgctg gcccacttgc acctctccac
     3061 cgggcagccg gtcgccatgg ccgcgggtaa cctgctggcc gacttcatgc gccgctttgg
     3121 cgccacggac atagacccgg aatttgccgc cggggtacgc ctgcaccgcg ccattgatgt
     3181 cttcaccgat tcccatccgc ttatccgcgc cgcgcgccgg ctcattgccc cgccgcgcaa
     3241 ccggctggcg ggcatcatta ttgatatcgc cctcgaccac cagttgtcgc gccactggaa
     3301 cgcccatgcc gcactgccgc tgaccacctt cgtgcgcgcc cgcctcgccg aaatccaagc
     3361 ctatctggcc gcgcacgata ccccgcttaa ctgggtcatc cgccgcgcca tccgcgatga
     3421 ctggctgctg tcctacgcca ccttggcggg tctggacctg accttcgaac gcgtggcctc
     3481 ccgcgccccc gtcgccgccg ctctgcgcgg cgcaaccgcc gacatcgcca ggcacgatgc
     3541 ccacttccaa cagctattcc acgccttcta ccccgctctc cgcgcccacg ccgcccaaac
     3601 ccacgccgcc ctcgcggttt gagctccccc cccccccccc ccccgccata atatcagttt
     3661 atgaaactga ccccagccat tgcgccttct ttgagattga tggcctaaca aagtggtgga
     3721 gaggcacgcc ttgacccatg catattcata tggcatattc atgagcatga gaacaaccat
     3781 catcatcaaa gaagagatcc tctccaaggc ctctgctttg acgggagtta aagagaagac
     3841 tgcccttgtc cacatgggac tggaagccct tattgcacga gctagcgcgg cgaggcttgc
     3901 cagactgggc agatcggagc ggggattgcg gcctatccca cggcgcaggg ttccataaat
     3961 gatgcttgtt gacacttccg tatgggttga ccacttgcgc aatggcaatg acgccttggc
     4021 cgatttgtta ttgagggacg aagtggcatg ccatccaatg gttatcggcg aattggcctg
     4081 tggaaatctc aagtgccgca aagaaattct ggcgttgctt caggctctcc ccacggtaga
     4141 atgcgtctct gacgatgaga tactcttttt catcggacaa caccgtctat ccggactcgg
     4201 cctgggattg gtagatgttc atcttcttgc atcctgtgct attgcccata cttcgctatg
     4261 gacactcgac agccggcttc aacgagccgc agatgaactt tcaaacaaat aagcgaacaa
     4321 gacgtttcac cgtacggcac actacgtgca ccgcgcggag agcgctgacg tccgaaaaaa
     4381 tattctttca cttgacaggt tataactaat gggttataga acggtaaggt tataaatata
     4441 tgtgggaaat tgaatatgtt ggagagttcg aattttggtg gaatcacctc tgaggacgag
     4501 caggatgaca tagcggctta tgtaaaattg cttgaagaaa agggaccgtc tctacccttt
     4561 ccgtatagtt caggcgttga gggttcacgg cacaatcata tgagagaact tagaatccaa
     4621 catgcgggtg acccttatcg ggtgctatac gcgtttgacc cgcgaagaac ggcgctgttg
     4681 ttattgggcg gaaataagaa aggcaatgat agatggtacg aagaaaatgt gccgattgcg
     4741 gataaattgt atgacaatca tcttgcccaa ctgagaaagg aaggactgat atcatgagca
     4801 gaccgttcaa aaaacttctt gaaaatatgc cgagggatcg ccgtgagcgc attcgcttaa
     4861 aaactgaggt cctaaaaaac gaaatggcac taaaagattt gaggcaagcc cttaaactga
     4921 cgcaagatga attagcccgg tcactaaaaa tgaaacaggc cgctatttct aaatttgagc
     4981 gtcaatccga tatttatctt agcacactgc gcaagatcct cttcgttatg ggtgctgatt
     5041 tgaagatcgt tgcccgtttc ccagaaggcg atgttctgat caatcagttt aatgacatta
     5101 cttcagcaaa tacccctgaa gatattgccc ccgtttcata aagagtattg aacaaggcgt
     5161 tctatgaaag agaaaaagat gaaatcacca accaagaaga aagcggacaa gccgcgctgt
     5221 gggctctgcg gcaaaaccaa gaacctgata aagaccgaat gctgcggtaa ttggatctgc
     5281 gacgacgaac acaagtatgt gatgttttcg tacgcaagaa acagttgctc cagaaatcac
     5341 cgacgttaca ccctctgtgg ctatcattac aatgagggcc ataaaggcga ctggaagacg
     5401 tgcaagaagt gtcgctctga ttttgaacca gagatggtcg tctggtacgg aaccaacgag
     5461 tataattttg aaaaaatgcc cgatccgcca tcgttcactc caacaaaatg ttcaaaatgc
     5521 ggacaggtaa ttgtactggg caatggcggg tatgcccatg gccctgacgg atatacatgc
     5581 ggcgagtgtt ttgcaaaaga taaccggagg aaatgaagca tcgaacaagg tgctcctggc
     5641 catcagcgtc aagccgcctc agctttatcg ctgatattgg ttgagaagac cgtatgccga
     5701 tgccgggatg ccgccgccct gcgcggcgcc accgccgaca tcgccacgca cgatgcccac
     5761 ttccaacagc tattccacgc cttctatccc gccctccgcg cccacaccgc acaaacccac
     5821 gccgcacgcg cggtttgagt tgcttcctac cgcatcagac ttggtgaagc agacaaaatg
     5881 tcgcaggggg agcgggcttt ttggcgcgcg agggtgtggc tttggataaa tcggggaggc
     5941 aaaagacaca caatccccgc catctggcgt attttatgtg atgtgctgca aacttggcat
     6001 ggcttctgct ccatgaaagg tgccatgaca gaccaagcaa caatccagca gcccaagccc
     6061 gccggcgatc gccgcctgat gcggtggatt ctggtgctgc aactggttat cttgggctgg
     6121 ctggccaaaa attactttga cggggcattc aaccgcagac accacaaccc acctgcggtc
     6181 gcaacccaac cggacactcc cccctccttg gagccctccg cacaaccgaa tgccccgtca
     6241 cctttttccg tactgccgcg ctggaccgcc ttccagcgcg ccacaccccc gccccgcctt
     6301 gtccacccga tggaccgcat ccgtctcgag atggagcgca tgatggcgga agccaaccgc
     6361 gccgccgcca attttgacgc gctgtttggc ttggacgact ctctctggtc cgcgctgccg
     6421 gcctcgcccg ccatgaacct gcgcgagctg cccgacgcct atgaactttc cctcgcgctg
     6481 cccaacaccg atcccggcag cgtggatgtg cagctcgatg gccggctctt gagcgtcacc
     6541 gcgcagcaaa acacgcagac cgcgcattcg acctccgcgc agcgcttcag ctcgcgcgtg
     6601 ctgctgccgg ggccggtcgc ggccaacgcc gcctgccagg tcaccaacga aaacgaccgg
     6661 ctctgcatcc gcattcccaa agccaccgcc ccgcaaactg cggcccgcca accctgaccc
     6721 ttgccggtcg tgaggagaac accatgagcg ccaccggaat tacggccatc aatgaaaaag
     6781 taaaagaaga cagcgccttt gtcgcggcgt tgcgcgctga gctggagaag gtcatcgtgg
     6841 gccagcgcta tttgctcgac cgcctgatga tcggcctgct ggccaacggc cacctgctgc
     6901 tggaaggcgt gcccggcttg gccaaaaccc tcgccgtcaa aacgctggcc gccgccatcc
     6961 acacctattt ccaccgcatt caattcacgc ccgatctgct gcccgccgac ctcgtcggca
     7021 cgcaggtcta taaccccaag acgggcgact tcggcacccg caaaggcccc atttttgcca
     7081 acattatttt ggccgacgaa atcaaccgtg cgccggccaa ggtgcaatcg gcgctgctgg
     7141 aggccatgca ggagcggcaa gtgaccatcg gcccggaaac gttccggctg ccggacccgt
     7201 tcctggtgct ggccacgcaa aacccgattg agcaggaagg cacctacccc ctccccgaag
     7261 cgcaggttga ccgcttcatg ctcaagctcg tggtcaagta tcccacgcgc gaagaggagc
     7321 gccgcattct ggaagccaac gcccgcacgg aagtcactca caacgtgcgt gccgtggtgg
     7381 agcccgacgc catccgccgc gcccgcgcca ccgtggacga aatctacgtg gacgacaaaa
     7441 tcaaggacta catcctctcc atcgtcttcg ccacgcgcga ccccgccgcg cacaagctcg
     7501 accagcttgc cgacgccatc cgctacggcg cctccccgcg cgccacgctc tacctgaccc
     7561 tcgccgcgcg cgcccacgcc ttcctccaag gccggctacg tcaccccgca ggacgtcaaa
     7621 tctatcgccc ccgatgtcct gcgccaccgc atcatcccct ccttcgaggc cgaggccgac
     7681 gacctgaccg ccgacgccat catcaccacc ctgctcaacg aactccccgt gccgtaaggg
     7741 ggccccgccg gcccgaatcc gccatgattc cgcgcgacat cctcaaaaaa atccggcgga
     7801 tcgaaatccg cacccgccgc gcggtccacg atgtcttcgg cggacgctac cactcggtct
     7861 tcaaggggca gggcatggag ttccaggaag tgcgcgaata cgtccccggt gacgacatcc
     7921 gcagcattga ttggaacgtc accgcccgca ccggcaaccc gcacgtcaag aaattcacgg
     7981 aagagcgcga gctgacggtg atgctgctgg tggatatttc cgcctccaac cttttcggct
     8041 ccaccgcgca gctcaagcgc gacctcgccg ccgagctggc cgccgtcctc gcgttttcgg
     8101 ccatcaccaa ccacgaccgc gtggggctga ttctcttttc cgaccgcgtg gaaaaacaca
     8161 ttccgcccgc caagggcccc acccacgtcc tgcgcgtgat ctcggaagtc ctcaacgcgc
     8221 gccccgtctc gccgcgcacc gatcttgccc ccgccctgcg ctacctcaac caagtcgtcc
     8281 gccgccgttg cgtcgcgttc ctcatttccg acttcatcgc ccccgactgc gaccggctcc
     8341 tgcgcaccac cgcccgtcgc cacgacctcg tcagcctgat cattggcgac aaacacgaac
     8401 acgccctccc cgccgtcggc ctcgtggatt ttcaggatgc ggaaaccggc cgccgtctct
     8461 ggctggacac cagccacgcc cccacccgcc gcgccctgct cacccggcag gccgaacgcc
     8521 gcacgcggct gctcgcccaa ctggcccgcg ccaattccga cgccatcgaa ctgcacgccg
     8581 gcgagcgcta cgaccgcg
//
LOCUS       DAOPNP010000107         8244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_9703_length_8244_cov_9.351081, whole genome shotgun sequence.
ACCESSION   DAOPNP010000107 DAOPNP010000000
VERSION     DAOPNP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9703_length_8244_cov_9.351081"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..989)
                     /locus_tag="PKI64_09030"
     CDS             complement(45..989)
                     /locus_tag="PKI64_09030"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE01186.1"
                     /translation="MNLTSARIQIIGAETLAGAALAAATQARGGAFSLLSADDTNDWR
                     DAAAIEQGIASFRPDYLFVVAGESGGIALNQAHPARLMESNLRVVLNVLGTAARLRVP
                     RLMYLASSCCYPRDAAQPLRIESLMTGSLEPTNTAYATAKLAGIQFCAAVRQEHGLAW
                     FAAIPSTLYGPEDHFDPAQAHVATSLMIRMHQAKQQRLSVFSVWGTGTPRRDFLFSRD
                     FADAAFFLAGHYDAANGPINVGPDSDYSIAEVARMVQQVVGYEGRLEFDSTKPDGAPI
                     KRLDASALRSLGWQPKTGFVDGLRETYAAFLKQLPVGL"
     gene            1018..2214
                     /locus_tag="PKI64_09035"
     CDS             1018..2214
                     /locus_tag="PKI64_09035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE01187.1"
                     /translation="MSKSKPVVVYLHRYPPEIEAFQWPALRDLAAELTPAYDLVYASM
                     MPAGRRDEELRRQMRVLELPFTVDQTHGRDKWWKTLRWYRQMPRLLRKIEELSPAVIL
                     CKETLPMIPGQVVRLKRPTIIATSDWWWSILFGQWRWGRRLADWMEAREVRSWNQPWV
                     RATVSTNAEKNLLAAKGMDAARIPVINAPQNPGIFRPLAPRPTKGELGLNEYEKHFAI
                     FGIIRGGKGYDELLDWWQKLQGRHPDWKLVIIGGAGGEAWCRQQIARRKLNDAVHMTG
                     WLPTKQEVNRWLNAMDGIIVHRRNSVDNQGIIPSALFNGLSTGRPVVASGLPGIAEII
                     RDGTDGYLYKPDDGDSFMATLERVLNHSAEATRIGQAGQLRAAECFNPRRAAQEHRAL
                     IDALVE"
     gene            complement(2267..3451)
                     /locus_tag="PKI64_09040"
     CDS             complement(2267..3451)
                     /locus_tag="PKI64_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOE01188.1"
                     /translation="MTLRLPLMENNITAEDRAAVIEFLRGDPILTQSENVRAFEQEWS
                     QWLGLQRSVFVNSGASANFITMAAVRHLYGPGEVIVPTLTWVSDIASVLAAGLTPVFM
                     DVNPRTLAMEPGQVIAALNQNTRAVFLTHCQGFDGLDDALLTELKRRNVPLIEDVCES
                     HGATHHGKKAGTFGLAANFSFYFAHHMSTIEGGMISTDDEDFYQACRMLRSHGMVRES
                     DNPAFRKKWEEANPDLNPKFIFAWPTGNFRSTEINAVIGRNQLKRLDANNRQRTENFR
                     LFLSLIDPELYWTDYKLEGSVNYAFPLVLRKADDSLRDRLMALMDANGIEHRRGNAGG
                     GNQLRQPYLHGIAPAKADIPRQFPVVDHIHFYGFYIGNYPTLEEARIRWLCDILNSLR
                     TA"
     gene            complement(3448..4449)
                     /locus_tag="PKI64_09045"
     CDS             complement(3448..4449)
                     /locus_tag="PKI64_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HOE01189.1"
                     /translation="MIITRTPYRISFFGGGTDYPAWYREHGGAVLATAIARYCYITCR
                     WLPPFFVHKSRIVYSIIEDIMRVEDIQHPAVRECLLQLKIHDGIEIHHDGDLPKMTGL
                     GTSSSFTVGLLQALYALRGEFRSKMQLAEEAIHIERNRCGDRVGSQDQVSAAFGGLNH
                     IRFAQDDSIAVHPIPLPATRLATLQQSLMLFFTGFARVASEVVGEQLENLPRKTKELT
                     TMGAMVDEGVRILMQGEMDDFGRLLHESWMLKRSLSSRVSTAEIDEMYGCARKAGALG
                     GKITGAGGGGFLLLYARPERHAAIRRALGHLLHVPFDIDHAGTQIIFSNPTPQEKST"
     gene            complement(4558..5490)
                     /gene="epmA"
                     /locus_tag="PKI64_09050"
     CDS             complement(4558..5490)
                     /gene="epmA"
                     /locus_tag="PKI64_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOE01190.1"
                     /translation="MRPRPADSDALRQQRRVLELRAKLHAAIRCFFVERGFLEVTTPL
                     ILPANAPETHIDAIAAGTGWLRTSPELAMKRLLAAGHEAIFQLGPCFRAGEQGRWHHP
                     EFMMLEWYRAPAGYLDILADTKALLAFVTTELRGGTDFSWQGKSLSCAQDMWEKYTVS
                     QAFIQFAGWDPAVRFDADRFDLDLVTRVEPGLPMDRPVVLLDYPTPQASLARRKPDDP
                     ARAERWELYLGGVELANAYSELNDPVEQRTRFAEANAARQRRGESAYPLDEAFLAALA
                     EMPPAGGIALGVDRLLMILADADSLDEVLPFRHG"
     gene            complement(5505..6074)
                     /gene="efp"
                     /locus_tag="PKI64_09055"
     CDS             complement(5505..6074)
                     /gene="efp"
                     /locus_tag="PKI64_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOE01191.1"
                     /translation="MAYTAADLRKGLKIEIDGIPYEVTEFQFVKPGKGQAMYKCRIKN
                     MIAGNTMEKTFRSVDKIDQPNIESKTLAFSYQEGDKYIFMDNKTYEQVELHKDVLAGQ
                     HHFLLEDLECEVLFFNGRAMSVTLPAFVIKEIKETEPGARGDTATNVLKPAKIDTGYE
                     IGVPLFINQGDFIRIDTRTGKYVERVKQS"
     gene            complement(6222..7622)
                     /locus_tag="PKI64_09060"
     CDS             complement(6222..7622)
                     /locus_tag="PKI64_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01192.1"
                     /translation="MRKVGVFLPWLIFFTLMVGKLALLQQTPLPDDISGDEIGYVAKA
                     RYFYQHGSFPPLTSAEDGALQYSDYRPPGYPTLISLLLPFGDEITQLRHSVNLVQYAM
                     DLLVTTLLLVVAFQFSQAKGFRIVAALILGIQPWTSAHIASLYADTLAMFLTVAGVVC
                     LAMFGHFRWVLARTTSITMGSFLLSLTATVRPEMIVLAPALLVLAGWLEIRRMPWRVW
                     LGYATLAALPFLLVVGLNMAYHWQTEKHIRIIAHFRHPTPGLNLWTKTWIGPQWLKEE
                     LLFGGLTRTRTDEEHVRLLAKYALPDPMQCDATIRIIHDVQSRGFMTAEEDDIFAEIA
                     AARIAAAPWTYYMWGRVYNSAYLWLNLSSATHYLTAQARCPRLVSKSITAGLFGMKIF
                     ILIFATGGMLSVWRNKGILRTSWPGRFVLIGAACIVLRTLFFGFVFNTAEFRYALVAW
                     PLMLAVALYGLARGWT"
     gene            complement(7627..>8244)
                     /locus_tag="PKI64_09065"
     CDS             complement(7627..>8244)
                     /locus_tag="PKI64_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOE01193.1"
                     /translation="THAHAAMAQLPRKPHQILCVSAMAPHKNAETLVRAFQLVRTTIP
                     AARLVLAGGWPDAAYERKISRLIATLGLCDAVEFAGFVSRERLEQLYAESRVFCLMSR
                     CESFGIPAIEAQLFGTPVVSSTVCAIPEICGSGGKFCDPDDVSAVAAALRQLLQDDTE
                     WQRLATAARTNARQYTWEACSAPLVDLLRTFCAAPATAMSPPAKG"
BASE COUNT         1847 a         2360 c         2438 g         1599 t
ORIGIN      
        1 ccttcccgcc gggaaaggct ggaaggtctg cgaccgttcc agccctacaa tccaaccgga
       61 agttgtttga gaaaagccgc gtaggtttct cgcaatccat ccacaaaacc ggttttcggc
      121 tgccagccaa gcgaccgcag cgccgaggca tccaagcgtt tgatgggcgc gccatcgggc
      181 ttggtggaat caaattccag ccggccctca tagccgacaa cttgttgcac catccgggcg
      241 acttccgcga tggaataatc gctgtcgggg ccgacattga tcgggccgtt ggccgcatcg
      301 taatgcccgg caaggaagaa tgcggcatcg gcaaaatcgc gggagaacag gaaatcgcgc
      361 cggggagtgc ccgtgcccca cacagagaaa accgacaagc gctgctgctt ggcctggtgc
      421 atgcggatca tcagcgaagt cgccacatgg gcttgcgcgg gatcaaaatg gtcctctggc
      481 ccataaagcg tggaaggaat cgccgcgaac caagccaggc cgtgctcttg gcgcaccgcg
      541 gcacaaaact gaatgccggc caacttcgcc gtggcatagg ccgtattggt gggctccaaa
      601 ctgcccgtca tgaggctctc gatacgcaaa ggttgcgcgg cgtcacgggg atagcagcag
      661 gagctggcca aatacatcag ccgtggcacg cgcaatcgcg cggctgtgcc cagcacattc
      721 agcaccacgc gcagatttga ttccatcaac cgcgcgggat gggcttggtt cagggcaatg
      781 ccgccagatt cgccggcaac cacaaagagg taatcgggcc ggaacgaggc aatgccctgt
      841 tcgattgccg ccgcatcgcg ccaatcattg gtatcatcgg cagacaaaag actgaacgcc
      901 cctccccgcg cttgggtggc ggcagccaat gcggctccgg ccaaggtctc ggcgccaatt
      961 atttggatgc gcgcgctggt caaattcatg ggaacagggt atagcatgga tgtcgccatg
     1021 agcaaatcga aacccgttgt tgtctatttg catcgctacc cgccggaaat cgaagccttc
     1081 caatggccgg cccttcgtga tttggcggcg gaactcaccc cggcctatga cttggtctat
     1141 gccagcatga tgccggccgg ccgccgggat gaagaattgc gccgccagat gcgcgtgttg
     1201 gaattgcctt tcacggtgga tcaaacccat ggccgcgaca aatggtggaa aaccctgcgt
     1261 tggtatcggc aaatgccccg gctgctccgg aaaatcgagg agttgtcgcc ggcggtgatt
     1321 ctttgcaagg aaaccctgcc catgattccg gggcaagttg ttcggctaaa gcgaccgacc
     1381 atcatcgcca ccagcgattg gtggtggtcc atcctgtttg gccaatggcg ctggggccgc
     1441 cggttggcag actggatgga agcaagggag gtccggagtt ggaatcaacc atgggtccgg
     1501 gccacggtca gcacaaacgc ggaaaaaaat ttgctggccg cgaaaggaat ggatgccgcc
     1561 cgcattcccg ttatcaatgc cccgcaaaat ccgggcattt tccgcccgct ggctccccga
     1621 ccgaccaaag gggaactggg tctgaatgaa tacgaaaaac acttcgctat ttttggaatt
     1681 attcgcggcg gaaaaggcta tgacgaactg ctggattggt ggcagaagtt gcaaggccgg
     1741 catcctgact ggaaattggt gatcattggc ggcgccggcg gcgaagcgtg gtgccggcaa
     1801 caaattgccc ggcgtaagtt gaacgatgca gttcacatga ccggctggtt gcccaccaag
     1861 caggaagtga accgttggct caatgcgatg gatggcatca ttgtccatcg gcgcaattcc
     1921 gtggataatc aggggattat ccccagcgcg ctgttcaatg gcctgtccac cgggcggccc
     1981 gtggtggcga gcggtttgcc gggaatcgcg gagatcatcc gcgatggaac agacggatac
     2041 ttatacaagc cggacgatgg cgacagcttt atggctacgc tggagagggt gctgaatcat
     2101 tcagccgagg ccacccgcat cgggcaagcg gggcagttgc gcgccgcgga atgcttcaat
     2161 ccgcgccgcg ccgcgcagga acaccgggca ctgattgacg ccttggtgga gtagccttcc
     2221 accgcccatg gggagcggga cgcggcaaag cggcatcaac ccaaatctat gccgtccgca
     2281 gggagttgag gatatcgcac aaccagcgga tgcgcgcctc ttccagcgtg gggtagttac
     2341 caatgtaaaa cccgtaaaag tggatgtggt ccacaacggg aaattggcgg gggatgtcgg
     2401 ctttggccgg cgcgatgcca tggagataag gttggcggag ctggttgccg cccccggcgt
     2461 tgccgcgccg atgttcaatg ccattggcat ccatcagggc catcagccgg tcgcgcaggc
     2521 tgtcatcggc cttgcgcaga accagcggaa aagcgtagtt cacgctgcct tcgagtttgt
     2581 agtccgtcca gtaaagctcc ggatcaatca gggagaggaa tagccggaag ttttcggtgc
     2641 gctgccggtt gttggcatcc agtcgcttga gttggttgcg cccaatcacg gcattgattt
     2701 ccgtgctgcg gaaattgccc gtgggccagg caaaaataaa cttgggattg aggtcaggat
     2761 tggcctcttc ccattttttc cggaacgcgg ggttgtcaga ttcgcggacc atcccatggc
     2821 tgcggagcat tcggcaggct tgataaaagt cttcatcgtc ggtgctgatc atcccgccct
     2881 cgatcgtgct catatgatgg gcaaagtaga acgagaagtt ggcggcgagg ccgaatgtgc
     2941 ccgccttttt tccatgatgc gtggcgccgt gcgactcgca cacgtcttca atcagcggga
     3001 cattgcgccg cttcaattca gtcaacaggg catcatccag cccgtcaaac ccctgacagt
     3061 gggtcaggaa aaccgcgcgg gtattttggt tcagcgccgc gatgacttgc ccgggttcca
     3121 tggccaacgt gcgcggattc acatccatga aaaccggggt caggccggcg gccagcacgg
     3181 aggcaatgtc ggacacccac gtcaaagtgg gcacaatgac ttcgccgggg ccgtacagat
     3241 gccgcaccgc cgccatggta atgaagttgg cggaggcgcc ggagttgacg aacacgctgc
     3301 gctgcaggcc cagccactgc gaccactcct gctcaaaagc gcgcacattt tccgactgcg
     3361 tcaggatggg atccccccgc aaaaactcaa tgacggccgc ccgatcttcg gccgtgatgt
     3421 tgttttccat caggggcaaa cgcagggtca tgtggatttc tcctgaggcg tgggattgga
     3481 aaagatgatc tgggtgccgg catgatcaat gtcaaacggg acatgcagca agtggcccag
     3541 cgcgcgccga atggcggcgt gccgttccgg gcgggcatag agcagcagga atccgccgcc
     3601 gccggcaccg gttatttttc cgcccagcgc cccggccttg cgcgcgcagc cgtacatctc
     3661 gtcaatttcc gcggtgctga cgcgcgaact gagcgagcgc ttgagcatcc agctctcgtg
     3721 cagcaagcgc ccaaaatcat ccatctcccc ctgcatcaga atgcgaacgc cctcatccac
     3781 catggcgccc atggtggtca gttccttcgt ctttctgggc aggttttcca gttgttcgcc
     3841 caccacttcg gaagccacgc gtgcaaagcc ggtaaaaaac agcatcagcg attgctggag
     3901 cgtggccaga cgggtggccg gcaacgggat gggatgcacc gcaatggagt catcctgggc
     3961 aaagcggata tggttcaagc cgccaaaagc cgcgctgact tgatcttgcg agccgacgcg
     4021 gtcgccgcag cggttgcgtt caatatggat ggcctcttcc gccaattgca ttttggagcg
     4081 aaactccccg cgcaaggcat acaacgcttg cagcaacccg accgtgaagg acgaggacgt
     4141 gcctaggccg gtcattttcg gcaagtcgcc gtcgtgatgg atttcaatgc catcatggat
     4201 tttgagctgc aggaggcatt cgcggacagc gggatgctga atatcctcca cgcgcatgat
     4261 gtcttcaatg atggaataga cgatccgtga cttgtggaca aaaaagggcg gcagccaacg
     4321 gcaggtgatg tagcaataac gcgcgattgc cgtggccagt acggcaccgc catgctcccg
     4381 ataccaagcg ggataatcgg tcccgccgcc gaaaaaggaa atgcgatacg gtgtgcgggt
     4441 gatgatcatg ccgggggttc caggcggagg tgattttgca gaatggcatt catgagttca
     4501 accggagccc acgatagggg caatgggcgg gaaaatgaag caaaaaccag cccggtttca
     4561 gccatggcga aaggggagga cttcgtccag cgagtccgca tcggccaaaa tcatgagtag
     4621 gcggtccacg cccaaggcga ttccgccggc gggcggcatc tccgccaagg cggccagaaa
     4681 agcttcatcc aatggatagg cggattctcc gcggcgttgg cgcgcggcgt tggcctccgc
     4741 gaaacgcgtg cgctgctcca ccggatcgtt caattccgaa taggcattgg ccagttccac
     4801 cccgcccaga tacagctccc agcgttccgc gcgggcggga tcatccggtt tgcggcgcgc
     4861 cagggaggcc tgcggcgtgg gataatccaa aagcaccacc ggacgatcca tcggcaggcc
     4921 cggctccacg cgggtcacga ggtccaaatc gaagcgatcc gcatcaaaac gcaccgctgg
     4981 atcccagccg gcaaactgaa tgaaggcctg agaaaccgta tatttctccc acatgtcttg
     5041 cgcgcaggag agagatttac cctgccagga aaaatcggta ccaccacgaa gttcggtggt
     5101 cacaaaagcc aacagggctt tggtgtcggc cagaatatcc aaataaccgg cgggggcccg
     5161 gtaccactcc agcatcatga actcgggatg atgccagcgc ccctgctctc ccgcgcggaa
     5221 gcaggggccc aactggaaaa tggcctcgtg accggccgcc agcagtcgtt tcatggccag
     5281 ctcgggcgag gtgcgcaacc accccgtgcc agcagcaatg gcatcaatat gggtttccgg
     5341 cgcattggcc ggcaaaatga gtggcgtggt gacttcgagg aaaccgcgct caacgaaaaa
     5401 gcagcggatc gccgcatgta gcttggcccg cagttccaga actcggcgct gttggcgcaa
     5461 ggcatccgaa tccgcgggcc ggggccgcat gatgctgcct atcctcagct ttgcttgacg
     5521 cgctcgacgt atttgccggt gcgcgtatca atgcggatga aatcgccttg attaatgaac
     5581 agcggcacac cgatttcgta gccggtgtca atcttggctg gcttgagcac gttggtggcg
     5641 gtgtcgccgc gcgcgccggg ctcggtttcc ttgatttcct tgatgacaaa cgccggcagc
     5701 gtgacgctca tggcgcgtcc gttgaaaaac aggacttcgc actcgaggtc ttccagcagg
     5761 aaatgatgct gaccggccag cacgtctttg tgcagttcga cctgttcgta ggtcttgttg
     5821 tccatgaaaa tgtatttgtc cccctcctga taggagaacg ccaaggtctt ggattcgatg
     5881 ttgggctggt caatcttgtc cacggaacgg aaagtttttt ccatagtgtt gccggcgatc
     5941 atgttcttga tccggcactt gtacatggcc tgccccttgc ccggtttgac gaactgaaat
     6001 tccgtcactt cgtagggaat accatcaatt tcaattttca aacccttgcg taaatctgct
     6061 gcggtgtatg ccatgatgcg atccttgtgc taactggttc caaaagcggc gtttgtttac
     6121 gccaaagcgc catttttgcc aaggaaaaag cttcgtgttg cgcctcacat cagcagcgca
     6181 tcttttgccg aaacgactac cttcgcgggc gagcccgcaa actatgtcca tccacgcgcc
     6241 agtccgtata gcgccacagc caacataagc ggccaggcca ccaatgcata tctgaactca
     6301 gccgtattga atacaaaccc aaagaaaagg gttcggagca ctatacaggc cgcaccaatc
     6361 aggacgaacc tgcccggcca acttgttctc aagattcctt tgttccgcca aacactcagc
     6421 attccaccgg tcgcaaaaat caaaataaat attttcatgc caaacaatcc cgcggtgatg
     6481 cttttactga cgaggcgcgg gcatcgggct tgcgccgtca aataatgcgt ggcgctgctc
     6541 aaattcagcc acaagtaagc cgaattatac acccggcccc acatataata ggtccacgga
     6601 gccgctgcaa tccgggcagc agctatttcg gcaaatatat catcctcctc ggcagtcatg
     6661 aatccgcgtg attgcacatc gtgaataatt cggattgtgg catcgcactg catggggtca
     6721 ggcaaggcgt atttggccaa aagtctcaca tgctcttcat cggtgcgtgt gcgagtgagg
     6781 ccgccaaata acagttcctc tttcaaccat tgtggcccga tccatgtttt ggtccaaaga
     6841 ttgaggccgg gtgtgggatg ccgaaaatgg gcaatgatcc ggatgtgctt ctcagtttgc
     6901 cagtggtagg ccatgttcaa accaaccacc aagaggaagg ggagcgcagc cagtgtggcg
     6961 tagcccaacc acacccgcca gggcatccga cgaatctcta accagccggc caaaaccagc
     7021 aatgcaggcg ccaggacgat catttccggt cgaacggtgg cggttaggct gaggagaaag
     7081 ctgcccattg tgatggacgt cgttcgcgcc aacacccagc gaaaatgccc aaacattgcc
     7141 aaacaaacga cacctgccac cgtcaagaac atcgccaatg tatccgcgta tagagacgcg
     7201 atgtgcgcgg atgtccaagg ctgtatgccc aagatcaaag ccgcgacaat ccgaaatccc
     7261 ttggcctggc taaactggaa tgccaccacc aataataatg tcgtaacaag caaatccatg
     7321 gcgtactgga caagattgac gctgtgtcgg agttgggtga tctcatcgcc aaagggaagc
     7381 agcagcgaaa tgagcgtcgg ataccccgga ggacggtaat cagaatattg caacgcgcca
     7441 tcttctgcgg atgttagcgg ggggaagctt ccgtgctgat agaagtagcg ggctttggcc
     7501 acgtaaccga tctcgtcacc cgaaatgtca tcaggcagtg gcgtttgctg taacagggcc
     7561 aacttcccca ccatgagcgt gaaaaagatc aaccaaggca aaaaaactcc gactttcctc
     7621 atgcccctac cccttcgcag gtggactcat tgccgtggcc ggcgcagcac aaaacgtgcg
     7681 caaaaggtct accagcggcg ccgagcatgc ctcccaggta tattgccggg cattggtgcg
     7741 ggcggcagtg gcgaggcgct gccattcggt atcgtcttgc aggagctggc gcagggcggc
     7801 ggcgacggcg gacacatcgt ccggatcaca aaatttgccg cccgaaccgc agatttctgg
     7861 aatggcgcaa accgtggaac tgaccaccgg ggttccgaag agctgggcct cgatggccgg
     7921 gataccaaaa gattcacagc ggctcatcag gcagaatacc cgcgattcgg catagagttg
     7981 ttccaagcgt tcgcgggaaa caaatccggc aaattccacg gcatcgcaca aaccgagcgt
     8041 ggcgatcagc cggctgattt ttcgttcgta ggcggcatct ggccacccac cagccaagac
     8101 caagcgcgcg gcaggaatcg tcgtgcggac aagttgaaac gcgcgcacaa gggtttccgc
     8161 gtttttgtgc ggcgccatgg ccgatacaca tagaatctgg tgcggcttgc gcggcaactg
     8221 ggccatggcc gcatgggcgt gggt
//
LOCUS       DAOPNP010000108         8178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_9795_length_8178_cov_9.526160, whole genome shotgun sequence.
ACCESSION   DAOPNP010000108 DAOPNP010000000
VERSION     DAOPNP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_8178_cov_9.526160"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1299
                     /locus_tag="PKI64_09070"
     CDS             217..1299
                     /locus_tag="PKI64_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE01194.1"
                     /translation="MKPSKSKTAKTKPFPVRRLYAVLLLLLAGLVWLLVQPQPGLRIA
                     RLKPVIAPEEPLPHAPPAQLRLATYNLENFTDGRNDGPERTPAVFVTHARDAAAVIAE
                     ANPDILLLQEIENRRTLEFLNSQLPEPYPYVYITKLRQSAGAREKLNLALMTRLRPRY
                     TRQLEFYYLAGKGRPARGVLAATFDLGDGSYLAVYGIHLKSNYGEAPRNQAQRGIALC
                     HIAADAIAETFRHDPAPLSVVILGDTNVDPEEPQFANDPSLEPLAGSFVDLWRGRPLA
                     ERITIPTRQPGPDGDPTMVFPPAAFDRVFVSKNLTSHGPWQALSPQAIQKGTDTTNNL
                     TLPGHNGHISDHFLVYTDLRSQAVAE"
     gene            1384..1458
                     /locus_tag="PKI64_09075"
     tRNA            1384..1458
                     /locus_tag="PKI64_09075"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1416..1418,aa:Cys,seq:gca)
     gene            1518..4214
                     /locus_tag="PKI64_09080"
     CDS             1518..4214
                     /locus_tag="PKI64_09080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOE01195.1"
                     /translation="MTNSYRQDAAAVLAAHASTPAGLTPAEAGARLAADGPNELRETV
                     SRPAWRMLLAQFIEPMILILLAAAGFAFVLGNVTEGLAILAIVVLFGILGFIQEYRAE
                     KAIAALKRLASPTVRVRRDNVLVEIPARDLVRGDIVYLETGNTVPADLRLLEAVNLRV
                     MEAALTGEAAAVLKQTAALTADQLPLGDRTNMAFLGTVVVNGRGAGVVVATGMKTELG
                     QIAGMIQGIAHARTPLQRKLAQVGKQISLLGLAAALAIFLLGFFRHEKIGELVLTAVS
                     LLVAVVPEGLPAVITATLALGSRRMLKRHALIRRLPAVETLGAVTVICTDKTGTLTEN
                     RMTVTHLHTLHGTMRLDTAALPAASRADNAELRTLLLTAALCNDAHLVFDEASSAPRA
                     MGDPTESALLIAAEQLGIATARLAGDAPRVAEYPFDAERKRMTTLHRSGDRGLPFPAV
                     MAAPVLAATKGSLDGLLARATHVVDAERVIPLTDEIRQQFLAHHERLAREGIRLLGVA
                     FRRFDAPPAPDTPADAVETDLVFCGQIGMMDPPRRAVCAAIASCHAAGIRTIMITGDH
                     PLTAVAIARGLHLAPAGQDPRVMDGTQLAALSAEELRQQVADVSVFARVSPTDKLRIV
                     TALQERGHIVAMTGDGVNDSPALKRAEIGVAMGITGTDVAKEAAQMVLLDDNYSTLVA
                     AVEEGRAIFDNLLRFLKYSFSGNLGKVLVMLAAPLAGIGVMALRPLHLLWLNLLTDGL
                     MGLGLGLEPAEADVMSRPPRRPDARILNRPALAHIGRMGLFICLGSLLLAGLWYRSPW
                     GHGEWQTVLFAAIGFAQVGQAWGLRALAPRPFRFGRNSALTGLTALTLLLQLGAMYIP
                     ALARVFNLQPLSGAGLAATIALGVVTFVLVHGERRLCLRRGC"
     gene            complement(4234..5052)
                     /gene="thiD"
                     /locus_tag="PKI64_09085"
     CDS             complement(4234..5052)
                     /gene="thiD"
                     /locus_tag="PKI64_09085"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOE01196.1"
                     /translation="MSTLKTYCRVLTIAGSDSGGGAGIQADLKTFAANGCYGMSVITA
                     LTAQNTQGVSGIHPVPPEFVAQQLDAVLADIGVDAVKIGMLFSPELIAAIARKLREYR
                     VAQVVLDPVMVAASGDKLLRDDAVAALKEHLIPLAALITPNLPEAAVLLDREIATREA
                     MASAATALADLGCQNVLVKGGHLVDGDSADCLYLGASRQIHWLASPRIATRNNHGTGC
                     TLSSAIAARLAQGDPMETAVRRAKDYITGAIQAGAEYAIGHGHGPVHHFWQVWA"
     gene            complement(5049..5738)
                     /gene="thiE"
                     /locus_tag="PKI64_09090"
     CDS             complement(5049..5738)
                     /gene="thiE"
                     /locus_tag="PKI64_09090"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOE01197.1"
                     /translation="MNLRRKKWNTARASRKDERVKPDYSLYLVTDRALARGRPLVELV
                     REAVAGGVTCVQLREKQLGTREMLAEARALLAVLRPAGVPLIINDRVDVALAAGADGV
                     HVGQQDMPLADVRRLTPRGWIVGVSAESLADAEQAARGGADYIGVSPVFATPTKTDTA
                     LPLGLAGLRAIRAAVQTPLVAIGGIHPDNAAAVLRAGADGLAVVSAILAAENPRQTAA
                     DLRRIIQETQA"
     gene            complement(5686..6501)
                     /gene="thiM"
                     /locus_tag="PKI64_09095"
     CDS             complement(5686..6501)
                     /gene="thiM"
                     /locus_tag="PKI64_09095"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOE01198.1"
                     /translation="MNTTTTLAARAAANLQRVRDTRPLVHNITNQVTINFVANVLLAA
                     GAAPVMAHAPNEVADMVALAKALVLNIGTLDDAQVAAMLHAGQAAARRGIPIVLDPVG
                     AGATPLRTATAQRILAETKVAVVRGNASEILALAGAAAQTRGVDAADSVEAAVAGGRE
                     LARATGAVVAITGPTDVIMDAQRVVRVTNGHPLMGCVTGTGCAATAVIGAFAAVDEDR
                     LLATATALAYFGLAGEYAADGAPGPGTFAVRFTDALHELTPEEMEHGARLTEG"
     gene            complement(6504..7760)
                     /gene="cytX"
                     /locus_tag="PKI64_09100"
     CDS             complement(6504..7760)
                     /gene="cytX"
                     /locus_tag="PKI64_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HOE01199.1"
                     /translation="MSTGEPNPTSERTLREWDFFLLWAGAAISLSEIWAGGLLASFGL
                     LTGLGIIVVGHLVGNTPMALGGLIGSRHGVPSMVSTRGALGNRGAMLPAVLNVLQLVG
                     WTSVMLWIGGQAAAGLLGGASSARWWIGGLGVLTTAWALAGSRMWRVLQKIGVVLLLA
                     LSVVMTVVALRAYGWRPLLAQSPPRTMPPMLGLDLVIAMPISWMPLVADYTRHAAVPG
                     RGLRGTWWGYFLVSSWMFAVGLMATLATGSAAPESMLLQLLGGHGLLAPAVAIVLLST
                     LTTTFLDIYSNALSAQSLFPRLGTRRLVLLGGLAGTLLALFFDATKYETFLLLIGSAF
                     CPLFGVVLTDYFILRRAHYEVADLYQRGRYWYRGGVNPRALLAWAVGFAIYQVCARSG
                     FVGGASLPALGAAALLYGGLARKQKG"
     regulatory      complement(7842..7952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1482 a         2973 c         2314 g         1409 t
ORIGIN      
        1 ctcgccgtta tctccgccac ggtgtgccag tccgccgcca tggcgctcgc cgcgcaacca
       61 atcatcaacc acaccgccag ccattgtgcc ttcatgttgc tccctgtgct catcgctcgc
      121 tcaatcacat tcgcgcgcaa catatcacaa ccccgcactg ctcgccaccg gtgttttcgc
      181 ttcccccgcg cgcgcgtttg tttctataac acccgcatga agccttccaa atccaaaacc
      241 gccaagacca aacctttccc cgtccgccgg ctctatgccg tgctgttgct gctgctggcc
      301 ggcctcgtct ggctgctcgt tcagccccag cccgggctgc gcatcgcccg cctcaagccg
      361 gtcattgctc ccgaagaacc gctcccccac gccccgcccg cccaattgcg cctggccacc
      421 tataatctgg aaaactttac cgatggccgc aacgacggcc ctgagcgaac tcccgccgtc
      481 ttcgtcaccc acgcgcgcga tgctgctgcc gtcattgccg aggccaatcc cgacatcctg
      541 ctgttgcagg agatcgaaaa ccgacgcacg ctggaatttc tcaacagcca actccccgaa
      601 ccctacccct atgtttatat caccaagctg cgccagagtg ccggcgcccg cgaaaaactc
      661 aacctcgccc tcatgacccg cctgcgcccg cgctacaccc ggcagcttga gttttattat
      721 ctggccggca agggccgccc cgcgcgcggc gtcctcgccg ccaccttcga tctgggcgat
      781 ggctcctatc tggccgtgta tggtatccac ctgaaatcca actatggcga agcgccccgc
      841 aatcaggccc agcgcggcat cgccctgtgc cacatcgccg ccgatgccat cgccgaaact
      901 ttccgccacg atccggcgcc gctctccgtg gtgattcttg gcgacaccaa cgtggacccc
      961 gaagaaccgc aattcgccaa cgacccctca ctcgagccgc tcgccggttc gttcgtggac
     1021 ctctggcgcg ggcgccccct cgccgagcgc atcaccatcc ctacccggca gcccggcccc
     1081 gatggtgatc ccaccatggt ctttccacct gccgcgtttg accgcgtgtt tgtttccaag
     1141 aacctgacca gccacggccc gtggcaagcc ctctcgccgc aagccattca aaaaggcacc
     1201 gacaccacca acaacctcac cctccccggc cacaacggcc acatctccga ccacttcctc
     1261 gtctataccg atttgcgcag ccaagccgtc gccgaataac cacccgcgcc gccactgctg
     1321 caaaatgcgc gcgcaagttg acaggcggcg gcgctcccgt tatctttccc ctttgacatg
     1381 aatggcgcgg tagccaagtg gtaaggcgag ggtctgcaaa atccttatac atcggttcga
     1441 tcccgatccg cgcctccagt ttccgttctg tccatcagcg ccgacaggcg cttttttggt
     1501 aaatggcgag ccaacgcatg accaactcct accggcagga cgcggccgcg gtgctagcgg
     1561 ctcacgcgtc cacgccggct ggcctgacgc ctgccgaagc cggcgcgcgc ctggctgccg
     1621 acggtcccaa tgaattacgc gagaccgtgt cgcgccccgc ctggcgaatg ctgctggcgc
     1681 agttcatcga gcccatgatc ctgattttgc tggccgcggc aggcttcgca tttgtcctcg
     1741 gcaatgtcac tgaggggctg gccattttgg ccatcgtggt gctgtttggt attctgggtt
     1801 tcattcagga gtaccgtgct gaaaaagcca tcgctgcgct taagcggctg gcctcgccca
     1861 cggtgcgggt gcgtcgcgac aacgtcttgg tggaaatccc cgcgcgggac ttggtccgcg
     1921 gtgatatcgt ctatctcgaa accggcaaca ccgtgcccgc cgacttgcgg ctgctggaag
     1981 ccgtcaacct gcgcgtgatg gaagccgcgc tgaccggcga ggccgccgcc gtcctcaaac
     2041 aaaccgccgc gctgaccgcc gaccaactac cgctcggcga ccgcaccaat atggccttcc
     2101 tcggcactgt ggtggtcaat ggccgcggcg cgggggtcgt ggtggccacc ggcatgaaaa
     2161 cggagctggg gcaaattgcc ggcatgatcc aaggcatcgc gcatgcccgc actccgctcc
     2221 aacgcaagtt ggcccaagtg ggcaagcaaa tcagcctgct ggggctcgct gccgcgctgg
     2281 cgattttttt gctcggcttc ttccgtcacg aaaaaattgg cgagttggtt ttgactgccg
     2341 tcagcctgtt ggtggccgtg gtgccggaag gattgccggc cgtgattacc gccacgttgg
     2401 ccctgggcag ccggcgaatg ctcaaacgcc atgccttgat ccgccgcctg ccggcggtgg
     2461 aaacactcgg cgccgtcacg gtgatttgta ccgacaaaac cggcacgttg accgagaacc
     2521 gcatgacggt cacgcacctg cacacgttgc atggaaccat gcgcctcgac accgccgccc
     2581 tccccgccgc ctctcgtgcg gacaatgccg aactccgcac gctcctgctc accgccgcgc
     2641 tttgcaacga tgcgcatttg gtttttgacg aggcatcttc tgcgccacgc gcgatgggcg
     2701 accccaccga atccgcgctg ctgattgcag ccgaacaatt gggcattgcc actgcccgcc
     2761 tggccggcga tgccccgcgc gtcgcggagt atccgtttga tgccgagcgc aagcgcatga
     2821 ccacgctgca ccgcagcggc gatcgcggcc ttccttttcc cgcggtaatg gccgcgcccg
     2881 tgctggcggc caccaaaggc tcgctcgatg gcctgctggc ccgcgccacg cacgtggtgg
     2941 acgccgagcg cgtcattccc ttgacggatg aaatccggca gcagtttctc gcacaccatg
     3001 agcgcttggc gcgcgagggc atccggctgc tgggtgtggc ctttcgccgc ttcgacgcgc
     3061 cgccggcgcc ggacactcct gccgatgccg tggaaacgga tctcgtgttc tgcggccaaa
     3121 tcggcatgat ggacccgccg cgccgcgccg tctgcgcggc gattgcctcc tgccacgccg
     3181 ccggcatccg caccatcatg atcaccggcg atcacccgct caccgccgtg gccatcgccc
     3241 gcggcttgca cctcgctcct gccggccaag atccgcgcgt catggatggc acgcaactgg
     3301 ccgcattgtc cgccgaagaa ctacgccaac aagtggcgga tgtttccgtt tttgcgcgcg
     3361 tttcgcccac ggataaactg cgcattgtca ctgccttgca ggagcgcgga cacattgtcg
     3421 ccatgaccgg cgacggcgtg aacgactccc ccgctctcaa gcgcgccgaa attggcgtgg
     3481 ccatgggcat caccggcact gatgtcgcca aagaagccgc ccaaatggta ctgctcgatg
     3541 acaactactc cacgctggtt gccgcggtgg aagagggccg cgccattttc gacaacctgc
     3601 tgcgtttttt gaaatattcg ttcagcggga atctcggcaa ggtgctggtg atgttagccg
     3661 cgccgctcgc gggcattggc gtgatggcgc tgcgcccact gcatttgctg tggctcaatc
     3721 tgctcacgga tggcttgatg ggtctcggcc tcggccttga accggcggag gcggatgtga
     3781 tgtcgcgccc gccccgtcgt cccgatgcgc ggattctcaa ccggcccgcg ctggcacaca
     3841 tcgggcgaat gggcctgttt atctgcctcg gctccctgct gctggccggg ttgtggtatc
     3901 gcagcccatg gggccatggc gaatggcaaa cggtgctctt cgcggccatc ggtttcgcgc
     3961 aagttgggca ggcgtggggc ctgcgcgcgc tggcgccacg ccccttccgt ttcgggcgca
     4021 actccgcgct gaccggcctg accgcgctga cgctactttt gcaactgggc gcgatgtata
     4081 tccccgccct cgcgcgcgtg ttcaacttgc aaccgttatc cggtgccggt ttggcggcca
     4141 ccatcgcgct gggcgtggtg acgtttgtgc tcgtgcacgg ggagcgccgc ctctgcctgc
     4201 ggcgcgggtg ttagactcgg cctccaccag ccgctacgcc cacacctgcc agaaatggtg
     4261 caccgggcca tggccgtggc caatcgcata ttccgcgccg gcctggatgg cgccagtgat
     4321 gtagtccttg gcgcggcgca ccgcggtttc catggggtcg ccctgcgcca gccgcgccgc
     4381 gatggcggat gacaacgtgc aacccgtgcc gtgattattg cgcgtggcga tgcgcggact
     4441 ggccagccag tggatttgtc gcgacgcgcc gagatacaga caatcggcgc tgtcgccatc
     4501 caccagatgg ccgcccttca ccaacacatt ttggcaaccc aagtcggcca gcgcggttgc
     4561 ggcgctggcc atggcttcgc gcgtggcaat ctcgcggtcg agcagcaccg cagcttccgg
     4621 caagttgggc gtgatcaagg ccgccagggg aattagatgc tccttgagtg cggccacggc
     4681 gtcatcgcgc aacagcttgt cgccactggc ggccaccatc accgggtcca acaccacttg
     4741 ggccacccga tattcccgca gcttgcgcgc aatggccgca atcagctccg gcgaaaacaa
     4801 catgccgatt ttgacggcat ccaccccgat atccgccagc acggcatcta gctgctgcgc
     4861 gacaaactcc ggcggcaccg gatggatgcc gctgacgccc tgcgtgttct gtgcggtcag
     4921 cgcggtgatg acgctcatgc cgtagcagcc attggcggca aaggttttca agtccgcttg
     4981 aatccccgcc cccccgccgc tgtcggaacc ggcaatggtc aatacccggc aatacgtttt
     5041 gagtgtgctc atgcttgggt ctcctgaatg atgcgccgta aatccgctgc ggtctggcgc
     5101 ggattctccg ccgccaaaat ggccgacacc accgccaaac catcggcgcc ggcgcgcaac
     5161 accgctgcag cgttgtccgg gtgaatgccg cctatggcca ccagcggtgt ttgcaccgcc
     5221 gcgcgaatgg cgcgcaagcc cgccagcccc aacggcagcg cggtgtccgt tttagtcggt
     5281 gtggcaaaca ctggactcac gccgatgtaa tccgcccccc cacgcgcggc ctgctcggca
     5341 tcggccagcg attccgcgga aacgccgaca atccagccgc gcggcgtcaa ccggcggaca
     5401 tcggccagcg gcatgtcctg ctggcccaca tgcacgccgt ccgcgccggc tgccagcgcc
     5461 acatccaccc ggtcattgat aatcagcggc acccccgccg gccgcaacac ggccaacagc
     5521 gcgcgcgctt ccgccaacat ctcgcgggtg cccaattgtt tttcgcgcaa ctgcacgcac
     5581 gtgacgccac ccgcgaccgc ctcgcgcacc agctcaacca gcggccgccc gcgcgccagc
     5641 gcgcggtcgg tcaccaaata caacgaataa tccggcttca cccgctcatc cttccgtgag
     5701 gcgcgcgccg tgttccattt cttccggcgt aagttcatgc agggcgtccg tgaagcgcac
     5761 ggcaaaggtg cccggccccg gcgcgccatc tgccgcatat tcgccggcca aaccgaagta
     5821 ggccagtgcc gtggccgtgg cgagcagccg atcctcatcc accgccgcga aagccccgat
     5881 gaccgccgta gccgcgcagc cggtgcccgt cacgcaaccc atcaagggat gcccgttcgt
     5941 cacccgcacc acgcgctgag catccatgat gacatccgtc gggccggtaa tcgccactac
     6001 cgcgcccgtg gcgcgcgcca gctcgcgacc gccggccacc gccgcttcca cgctgtccgc
     6061 cgcatccacg ccgcgcgtct gcgccgccgc accggccaac gccaaaatct cggaagcgtt
     6121 tccgcgcacc accgccacct tggtttcggc caggatgcgt tgcgccgtcg ccgtacgcaa
     6181 gggggtggca cccgcgccga ccggatcgag aacaatcggg ataccccgcc gcgccgccgc
     6241 ctgccccgcg tggagcatcg cggcaacctg cgcgtcatcc agcgtgccga tgttcagcac
     6301 cagcgccttg gccagcgcga ccatgtccgc cacttcattg ggcgcgtggg ccatcaccgg
     6361 cgccgcgccg gccgccagca aaacattcgc cacaaaattg atggtgactt ggttcgtgat
     6421 gttgtgcacc agcggccgcg tgtcgcgcac ccgttgcaaa ttggctgcgg cccgtgccgc
     6481 caacgtggtt gtcgtattca tgttcatcct ttctgtttcc tggccagccc gccatacagc
     6541 agcgccgccg cgcccagcgc cggcagcgat gcgccgccga caaacccgct gcgggcacaa
     6601 acctgataga tggcgaaccc gaccgcccat gcaagcaagg cccggggatt cactccgccc
     6661 cgataccagt accgcccgcg ctgataaagg tcggccacct cataatgcgc gcgacgcaaa
     6721 ataaaataat ccgtcagcac caccccgaac aacggacaaa atgcggagcc gatcagcagc
     6781 aaaaaggtct catatttcgt ggcgtcgaaa aatagcgcca agagggtgcc ggccaagcca
     6841 ccgagcaaca ccaagcgccg tgtgcctagc cgggggaaca aactctgcgc ggagagcgcg
     6901 ttggaataga tgtcgagaaa tgtggtcgtc aacgtggaga gcagcacgat ggccacggcc
     6961 ggcgccagca ggccgtgccc gcccagcaac tgcagcagca tggattccgg cgccgcggag
     7021 ccggtggcca gcgtggccat caaccccacg gcaaacatcc atgaactcac gagaaaatag
     7081 ccccaccacg taccgcgcaa accgcgcccg ggcaccgcgg catgacgcgt gtagtccgcc
     7141 accaacggca tccaggaaat cggcatggcg atcaccaagt ccaaccccag catgggaggc
     7201 atggtgcgcg gcggggactg cgccaaaagc gggcgccacc catacgcgcg cagggccacc
     7261 acggtcatga ccacggacaa ggcgaggagc agcaccaccc cgattttttg cagcacgcgc
     7321 cacatccggc tgccggcaag agcccacgcg gtcgtcaaca cccccagtcc cccgatccac
     7381 caccgggcgc tgctcgcgcc gcccagcagc cccgccgccg cctgcccgcc aatccagagc
     7441 atgacggagg tccagcccac cagttgcagc acattcagca cggcgggcaa catggcgccc
     7501 cgattgccca gcgcgccccg cgtgctgacc atgctgggta cgccatgacg actgccgatc
     7561 agtccgccca gcgccatcgg cgtattcccc accaaatggc cgaccacaat aatccccagc
     7621 ccggtcaaca acccgaacga tgccagcaac ccgcccgccc agatttcaga cagcgaaatc
     7681 gcggcacccg cccacaagag aaaaaaatcc cattcgcgca gcgtgcgctc gctcgtagga
     7741 tttggctcac cggttgacat tgcatcttct ccgatcctgc cgccggggag cctgccgcgg
     7801 ccaacaaaaa accgcggttc catcgaagaa accacggtgc cggtccacac gatccgctgt
     7861 ccctacgctg gaatgatcca acaggttcca agggttatcc ggccaaccgg aactctcagc
     7921 cctctccatc gaggacaccc ctagcgacca cattttttag tcaacgacag accataccac
     7981 ccatgccacc cgcgtcaagc ccggcccttt tccacactgt ggaaaaaagt ttatcccacg
     8041 caggcgggat tttccacacc gtggaaaaat ccaccgccac gcgcccattt tttccacacc
     8101 gtggaaaaaa gttttccacg ctatggaaaa accaggtcaa attgttccac acaatggaaa
     8161 aactttcggc ggattttc
//
LOCUS       DAOPNP010000109         8169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_9805_length_8169_cov_7.979172, whole genome shotgun sequence.
ACCESSION   DAOPNP010000109 DAOPNP010000000
VERSION     DAOPNP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9805_length_8169_cov_7.979172"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKI64_09105"
     CDS             <1..344
                     /locus_tag="PKI64_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01200.1"
                     /translation="FMGQWVGDTAPLEQDPEFLKQKEAAPEMVQQMLALMQGMKMSVT
                     LDGVTMEVMGEKAGPFGYSVIAAHDDILVIETQDGPKAGVRSRLTFRDARHVQVEELT
                     PAGRAMFFKKP"
     gene            complement(615..691)
                     /locus_tag="PKI64_09110"
     tRNA            complement(615..691)
                     /locus_tag="PKI64_09110"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Asp,seq:gtc)
     gene            complement(794..2323)
                     /gene="malQ"
                     /locus_tag="PKI64_09115"
     CDS             complement(794..2323)
                     /gene="malQ"
                     /locus_tag="PKI64_09115"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010998012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOE01201.1"
                     /translation="MKFNRASGVLLHPTSLPGAYGIGEIGPEAYRFADFLHATGQRLW
                     QILPLGPTSYGDSPYQSPSTFAGNPLWISFDLLVKDKLLTKAQLKNFPVFPAEKVDFG
                     AVIPARMAVLQQVCATFADRASPALQEQFAAFCARHAGWLDEYALFTALKDAHNGVPW
                     PQWEPALARREPAALQAARTQYAAEIRNVQIIQFLFADHWQRLRTYCAKLGIQFIGDI
                     PIFVAHDSADVWANPHLFYLRPDGQPSVVAGVPPDYFSATGQLWGNPLYNWAVHKEQG
                     YAWWLARLQRICELVDIVRIDHFRGFEAYWEIPGGETTAINGRWVKGPDQDLFDILRA
                     KMGDLPIIAEDLGVITPPVEALRDNNDFPGMRILQFAFGNDERSAEFRPESYPAHCCV
                     YTGTHDNDTTVGWFHSEAGNGSTRTAEDIAAERKMILDYLRTDGHEIHWDLINLGARS
                     NANTFVVPLQDLLGLGSAARMNTPGVAGGNWQWRFAWEQITDAIRERFSHVTRATGRR
                     V"
     gene            complement(2469..4073)
                     /locus_tag="PKI64_09120"
     CDS             complement(2469..4073)
                     /locus_tag="PKI64_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOE01202.1"
                     /translation="MDAQCAVSGATEVEYNGQLRTNDFIVYPEGIPYSVAYTPPAPRN
                     AGSYTATVTIPSTNQITGGVFVFSNSLVITKATPSCVLNAVVQTNYDGTAHTNGFTVT
                     PSELMWSVAYSPENPPVNVGSYDATVTIVGNSNWFDHEITFDGAVVIADSLPVPPQPR
                     VSLTNNNNFTVTWAGIPNAVSYRLDVSTSATFGSAGGTVYTVDFEDGTKTSFAEADVT
                     LNGKSWKLTEALIGTLAGDRRNGTKAARVRYNDPEYYGTGIMAMNEDLATGLSAVSLL
                     HGKYGDDPNTAGRVDYSTNGGTSWVSVGTFDATATNLTLFSVTNLNVAGNVRLRVVKT
                     SGNDKARFNVDDIQIYPYTPGSSFVAGYSNRAVMTTSQVVTGLQAGVTYYFRVAAIST
                     GGLGGYSPVASVTTRTGEPSGTPFENWLISRGHDPQSTDYSAEADSDGDGMTTWQEYL
                     ADTDPANSNSVLRLSGTLSAAEGRLRWTFPQSTARYYQVEYSTNLTQGLAVSNLGWGV
                     PGRMLTNAIPSSGEWFWGIRSLLDAP"
     gene            complement(4506..4805)
                     /locus_tag="PKI64_09125"
                     /pseudo
     CDS             complement(4506..4805)
                     /locus_tag="PKI64_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(5907..7304)
                     /locus_tag="PKI64_09130"
                     /pseudo
     CDS             complement(5907..7304)
                     /locus_tag="PKI64_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            7436..7510
                     /locus_tag="PKI64_09135"
     tRNA            7436..7510
                     /locus_tag="PKI64_09135"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7468..7470,aa:Asn,seq:gtt)
     gene            7730..8158
                     /gene="rplM"
                     /locus_tag="PKI64_09140"
     CDS             7730..8158
                     /gene="rplM"
                     /locus_tag="PKI64_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE01203.1"
                     /translation="MKTTQPKESEVKHAWYLIDAADKPAGRLAVDICRLLRGRGKPDY
                     TPHIDMGDFVVVINSAKVALSGNSKEEKKTYAKYTRYQGGYTETSAGKMRAKNPNYIL
                     WHAVKGMLPKNHMSRTLLKRLKLYPGAEHPHAAQNPTVIG"
BASE COUNT         1621 a         2375 c         2544 g         1629 t
ORIGIN      
        1 agttcatggg tcaatgggtc ggcgatacgg ccccgctcga acaggatccg gaattcctca
       61 agcaaaagga ggccgccccg gaaatggttc aacagatgct cgccctgatg caaggcatga
      121 agatgtccgt gaccttggac ggcgtcacca tggaagtcat gggcgaaaaa gccggcccgt
      181 tcggctacag cgttattgcc gcccacgatg atattctcgt catcgaaacc caggacggcc
      241 ccaaggccgg ggttcggtcg cggctcacct tccgcgatgc ccggcatgtg caggtggaag
      301 aactcacgcc cgctgggcgg gccatgttct tcaaaaaacc atagcccttg cccttgggat
      361 cagggaggcg ggaagggtca gtggagagct cagaggtcgg aggtcagaag tcaggagtcg
      421 gaggtcagag gtcaggtgaa ggccgctggg ccgacagcct cgcgcgggcg aggaagaaga
      481 taggacggat gaaaccgatc cggcggatag tttttgccgc aactgacccc gcccccatcc
      541 cccttgccgt ggaaaaacgt tagtttgggg gtaaaagaaa aacccccgga aatccggagg
      601 ttttcgctga tttctggcgg ggtcgacggg acttgaaccc gcaacaccca gatcgacagt
      661 ctggtactct aaccaattga gctacgaccc cgtagcgcaa aaatcacggc agaagatagc
      721 cgaaacccgt ggtcatgcaa gtagaaaatg cgcagatttc cgattgcatg agattggggt
      781 agcagcgcga acttcagacg cggcggcccg tggcgcgggt gacatggctg aagcgctcgc
      841 ggatggcatc ggtgatctgc tcccacgcga accgccattg ccagttgcca ccggccacgc
      901 cgggggtgtt catccgcgcg gcggaaccca agccgagcaa atcttgcagc ggcaccacaa
      961 acgtattggc gttggagcgc gcaccgaggt tgatcaaatc ccaatggatt tcgtggccgt
     1021 cggtgcgcag ataatcgagg atcattttgc gctcggcggc gatgtcttcg gcggtgcggg
     1081 tgctgccgtt gccggcctcg ctatggaacc agccgacggt ggtgtcgttg tcgtgggtgc
     1141 cggtataaac gcagcaatgg gcggggtagc tttcggggcg gaactcggcg gagcgttcat
     1201 cgttgccgaa cgcaaactgg aggatgcgca tgccggggaa atcgttgttg tcgcgcaacg
     1261 cctcgacggg cggcgtgatg acgccaaggt cttcggcgat gatgggcagg tcgcccatct
     1321 tggcgcgcag aatatcgaat aaatcctgat cggggccctt gacccagcgg ccattgatgg
     1381 cggtggtttc gccgccgggg atttcccaat aggcctcgaa gccacggaag tggtcaatgc
     1441 gcacgatatc caccaattcg cagatgcgtt gcaggcgcgc cagccaccag gcatagccct
     1501 gttccttgtg cacggcccag ttgtagagcg ggttgcccca cagttggcca gtggcgctga
     1561 agtagtccgg gggcacgccg gccaccacgc tgggctggcc gtcggggcgc aggtaaaaga
     1621 ggtgcgggtt agcccacacg tccgcgctgt cgtgcgcgac aaaaatggga atgtcgccga
     1681 tgaactggat gccgagcttg gcgcagtagg tgcgcaggcg ctgccaatgg tcggcgaaca
     1741 ggaattggat gatctgcacg ttgcggattt cggccgcgta ttgcgtgcgg gcggcttgga
     1801 gcgcggcggg ttcgcggcgg gccaaggcgg gttcccattg gggccagggc acaccgttgt
     1861 gcgcatcctt gagcgccgtg aacaaagcgt actcatccag ccaaccggcg tggcgggcgc
     1921 aaaacgcggc gaactgttct tgcaacgccg gagaagcgcg atcggcaaac gtggcgcaga
     1981 cttgttgcag caccgccatc cgcgcgggaa tcaccgcgcc aaaatccact ttctccgcgg
     2041 gaaacaccgg gaagtttttc aactgggcct tggtgagcag tttgtccttc accagcagat
     2101 caaagctgat ccacagcggg ttgccggcaa aggtggaggg cgactgatat gggctgtcgc
     2161 cgtagctggt gggacccagc ggcaggattt gccacaaacg ctgccccgtg gcgtgcagga
     2221 agtccgcaaa ccggtaggct tcggggccga tttcgccaat gccataggcg ccgggcagcg
     2281 atgtcggatg cagcaaaacg ccggaggcgc ggttgaattt catggggcca agctccttgt
     2341 aaatgggttt gatagcgagg cactatggca cagggcggcg cgcttgtcga agcgtttttt
     2401 cacaaggtaa aacgaaccgc aattgcgggt ttatgcggcg tttccgggcg gcaatccgcg
     2461 cgggccggtc agggcgcatc caagagcgac cggattcccc agaaccattc gccggagctg
     2521 gggatcgcat tggtcagcat gcgcccgggc accccccacc ccagattgct cacggccaag
     2581 ccttgggtga ggttggtgga gtattccact tggtaataac gcgcggtgct ttgcgggaag
     2641 gtccagcgca ggcggccttc ggccgcactc aacgtgccgc taagacgcag gacgctattg
     2701 gagttggccg gatcggtatc cgccagatat tcctgccacg tggtcatgcc gtcgccgtcg
     2761 ctgtcggcct cggcagaata gtcagtggac tgcgggtcat ggccacgact gatcagccag
     2821 ttttcaaatg gtgtgccaga aggttcgccg gtgcgggtgg tgacactcgc caccggggaa
     2881 tagcccccca gcccgccagt ggaaatggcc gccacgcgga agtagtacgt cacgcccgct
     2941 tgcaggccgg tgacgacctg gctggtggtc atcactgcgc ggttagaata gcccgcgacg
     3001 aacgagctgc cgggggtata cgggtagatc tgaatgtcat caacgttgaa gcgagcttta
     3061 tcattgccgc tggttttgac cacacggagc cgtacattgc cggccacgtt caagttggtg
     3121 acgctgaaca acgtcagatt ggtggcggtg gcatcgaacg tgcccacgct gacccatgaa
     3181 gtaccgccgt tggtgctgta gtccacccgc ccggcagtat tgggatcgtc gccatatttg
     3241 ccatgcagca agcttacggc gcttaagccc gtggccaggt cttcgttcat ggccatgatg
     3301 ccggtgccat aatattccgg gtcgttgtaa cgcacgcggg cggctttggt gccattgcgc
     3361 cggtcaccag ccaaggtgcc aattagtgct tcggtcaact tccatgattt tccgttgagc
     3421 gtgacatcgg cctcggcaaa gctggtcttc gtgccgtcct caaagtccac cgtgtacacc
     3481 gtgccgcccg cgctgccgaa agtcgcgctg gtgctgacat ccagccggta actgactgcg
     3541 ttgggaatac ccgcccacgt cacggtgaag ttgttgttgt tggtgaggct gacccgcggc
     3601 tgcggaggca ccggcaggct gtccgcaatg accacggcac cgtcaaatgt gatctcatga
     3661 tcgaaccagt tgctgttgcc gacaatggtc accgtggcat cgtaggaacc cacgttgacc
     3721 ggcggattct ctggcgaata ggccacggac cacatcagct ccgacggcgt cacggtgaag
     3781 ccgttggtgt gggcggtgcc gtcgtagttg gtttgcacca ccgcattcag gacacaggag
     3841 ggcgtggcct tggtaatgac gagagaattg ctgaaaacga aaacaccgcc cgtgatctgg
     3901 ttggtgctgg ggatggtgac ggtggcggta tagctgccgg cattgcgcgg cgcaggcggg
     3961 gtataggcga cggaatacgg aatgccttcc ggatatacaa tgaagtcgtt ggtgcggagc
     4021 tggccgttgt attccacttc cgtcgcgccg gaaaccgcgc attgggcatc catttcctct
     4081 tcgggcaaca tgaaactgct gatgacaatg tcatccaagc tggctcggta gtctttggtc
     4141 ccttcgcaca gaatccggaa ctccaccgga ccgagaacat tggccgtgac gcggttggtt
     4201 gccaagttgg cgacgccagt tgtgttgttg gaactgaggg tgatccagtc cgcgcctttg
     4261 cgatattggg caatcaatga cactggttcg ctggcatatt gtgcgtaagc aaacgacagt
     4321 tcaccgatcc cattggtcaa cattccttcc agcacgatgg cgccggaggt cttgatccga
     4381 caactgcgcg tgccgttgta ccgatcgttg gttgtctggt tcccgccggt gggaatcatc
     4441 gcattggtca gcacccaatt gcgcccgctc aactggacgg tggccgaggc gtacgcagcc
     4501 ttgttgcctt cctcaaaagt cacttgcaag gtgttcggcg tgtatggatc agggtcttcg
     4561 tcgtttaaga gcccatcgcc gtcaatatca tcgtcacatt cgtcgccaat cccatcgcca
     4621 tccagatcgg cttggtccgg attgtaggtg taggggcagt tgtcttgctc attcgcgatg
     4681 ccgtcgccgt caatatcggg atcgcattcg tcgccgaggc cgtcgccgtc ggtatcaatc
     4741 tgggtgggat tgaacgtatc gggacaatta tccaagtcgt cgggaatgcc gtcgttgtcc
     4801 gtatccaacc gcacaaccat cactgtgccg ttggtctggc gggcattgag tgtgcccggc
     4861 gtggcgggag cgacgtacca gttcgtggcg ctcaacacca cgttcggcgc ttgggggcaa
     4921 tcgttggagt ccgggtccac gccgatcaca tgcagatagt tcggactaac gctgggcacc
     4981 agccgggaca cggttcccgg atcgttgaca tcggtatcaa acttgttggt ggcgttggcc
     5041 agccatagca ccgcatccac aatattggcg gcggaatcat acaggatcaa ggcatgcggg
     5101 ccgttgccca gcgtattgcc ggcgggcagg gtcaaatcgg tattgggcac ataggcggga
     5161 tagcggctca acaccaccaa ccccagcgcc ttgccgccac gttcagtcag gccgtcgttg
     5221 ggcaccgtga acgaagggaa tgtgtaggtc caaaccgggc cgttgacatt ggttgcgccg
     5281 ttgtagtgaa cgattttata gcccagcagg ttggtgccgg cgggcgcaat cagttcaata
     5341 aactcgttgg tgtccgtggc gccggcgcca ttggcgcgca cctcgttgaa caatggcatg
     5401 aggcgggtgc cgctaccggc ctcggcgggg aatgtatagt tgttggtgct ggaattcttg
     5461 gggctgttat tccctggccc ggcgaactgg gcgcggacaa aatactccag aacatcgcca
     5521 cccgacttgg cgacactcaa ctgtggcgtc cgccagaggt tggcgccggc attggtcatg
     5581 gcattggtga tccacgcgcc gccggaaatc cggaagtgcg cccacaccgc aaaattggaa
     5641 gccgatatgt tgccggccac gtttgccacg atggcgaagc tgttggtggc ccgcggcacg
     5701 gcggggtcga gcgccaggcc ggagatggag acatcggcat cggccagggc ctggtgtcga
     5761 cccggcgtgg cggcattgat gccagcggtg atggtccatt cagcggcgga ccacgtcggc
     5821 aacggctcgc tgacgccgga cttacgcacc gcagtggagt tggtatagcc ccacgcacca
     5881 ggatagctgc caatcacgcc gtacacatcc accaccgtat tcgagcgcag tagagccacg
     5941 gcatcgttgc catccagccg cccgataact ggagcctgct ggttggcgtt tgtgccataa
     6001 aggatgcgat agttgttggt ggaggccgcc agcgtgtaca ccgcgcccgc cgccagcgtg
     6061 ccactcaacg aataggtggc cgtggcgcgc gtggccttat ccaaatagat cgccactttg
     6121 taagccgaca aatccaagct gccgttgccg gtattgcaga tttctacata gttgccattg
     6181 gtgttcgcga tatggtccac aagctcgctg atgatcccca ccggcgtcat gccggccact
     6241 tcgtcaatca gatggaaatc cgcaaaaacc atgcgatggt cggaggcgtt ggagctggtg
     6301 ttggccggca gcggcgaacc atacttggtc agccccacgt tggcgccgtc gccggcggaa
     6361 ttatatacct ccgcttgcgg cgcgccgtag gcgctttgca tgatttcctc gctgaacaac
     6421 aagtaatcca gccggtagcc ggtaccatcc gtgtgcgcgt gggtccacgt gttggttccg
     6481 ccggtgtgct tggccattac cgcgctcatg cccaccgtcc cgaggcggtc agtgggatag
     6541 tagcgatagg gcagcagcca attcgcgttg gtataccacg gcgtgtcact gccggccttg
     6601 aaggtggctg gcagtgagcc cagttgcgct tcataatagg attgcggaaa gttggtggtt
     6661 tgacccagac ccacgctgcc ttcgatggtg tcgttgaagt ctcccatcac ggcatattca
     6721 gtatccaacg gatactgcgc catcatggcg tcaatataat tgactagtcg gcggatttcg
     6781 aaagcgcggc gcatccgctc gcgcttggcc gtggtggagg acttgttgtg gacggaaaac
     6841 acgtgcagcg gattgagcgc gccgggcacc tgaattaccg cgtgcaacgg ccagcgggtc
     6901 atttctgccg cagtgggatc cactacggtc tccttcacct cgtccgaggc caggatggga
     6961 aatttgctcc aaatgccgag gcgcgaggtg ccggcaaacg tgccaccggc cggcgatgca
     7021 aacgcgtaat acgggaagcc cagagttccg gccatttgca cccaagccgc ctgatccgat
     7081 gtcgtcagtt cctggaaaca aacaatgtcc ggtttgacgc gctgcatggt ggccaacacc
     7141 gccgcgtaat cgtcatcggg attctggttg ccgcggtcgc cccccgtatc aatgccgaag
     7201 tacacattgt aggacgcaat gcgcacttgg accgcccatc ccgcaggtac gccacagaac
     7261 agcgccaccg ccagaaaaag ggctataacc gcgaactttt tcatgcgaga tggaatcgta
     7321 gcacccccac acccgctggc aaatacaatc ctaacatttt tcttgtaacc ttttaccacc
     7381 gcgccggagc ttgacattcc acgtcatccg gttatgtttg cccccgattt tcggttccag
     7441 cgtagctcaa tggtagagcg gttggctgtt aaccaatagg ttacaggttc gagtcctgtc
     7501 gctggagcca tttttcgcgt ttcgggcgcc tgttccggcg gtaaccggat ccggcgctgc
     7561 gcagggaaat ccatctgctg cgcaccgttc tgcgaaaggc gtcctctgtc gtcggatttc
     7621 ccgatccctc cggcacgggg ttgtttccat gcacatagaa agaaccgcgt tgacagccgc
     7681 gcgctccccg cgctactgtt cgccgctcat cgttgggaat tgaaggatta tgaagacgac
     7741 acagccgaag gaatctgaag tcaagcacgc gtggtatctc atcgatgccg ccgacaagcc
     7801 cgccggccgc ctcgcggtcg acatctgccg cctcctccgg ggccgcggca agcccgatta
     7861 cacgccccac atcgacatgg gcgatttcgt cgtggtcatc aactccgcca aggtcgccct
     7921 ttccggcaac tccaaggaag agaagaagac ctacgccaag tacacccgct accagggcgg
     7981 ctacaccgaa acctccgcgg gcaagatgcg cgccaaaaac cccaactaca tcctctggca
     8041 cgccgtcaag ggcatgctcc ccaagaacca catgagccgc acgctcctca agcgcctcaa
     8101 gctctatccc ggcgccgaac acccccacgc cgcccagaac cccaccgtta tcggctaatt
     8161 ccggagaaa
//
LOCUS       DAOPNP010000110         8035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_10035_length_8035_cov_8.609023, whole genome shotgun sequence.
ACCESSION   DAOPNP010000110 DAOPNP010000000
VERSION     DAOPNP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10035_length_8035_cov_8.609023"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1306)
                     /locus_tag="PKI64_09145"
     CDS             complement(164..1306)
                     /locus_tag="PKI64_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOE01204.1"
                     /translation="MKMQSIEWLRQLVALPSVNPAMSTDDAQRNEARLVDWLEPWFAA
                     RGFRVERVGQTPGRPNLLVRVGARQPRRTLLFESHLDTVGVAGFQGDPFTLREENGRL
                     YGRGACDTKGPLAAFMATLDDEVLAALATSSIELIWLGAIGEETGNLGAEEAIAAGLR
                     ADECVVLEPTDLQVVHAHKGVCWFTAATRGRAAHASDPSRGDNAILKMAQVWGILAEA
                     TAEAAKQYPGHVLGSPTISIGTIQGGSGTNIVPDRCEIQVDRRLMPGESAPAVFADLQ
                     QRLAVVPGGVELTLLKEGLPFHTPADAPLVREFSAALAAGGVPSVVTGAPWCSDAGPL
                     ALACGQTIVWGPGAIAQAHTVDEYIETAVLETARKVLRHFLLAAAK"
     gene            complement(1316..2209)
                     /locus_tag="PKI64_09150"
     CDS             complement(1316..2209)
                     /locus_tag="PKI64_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOE01205.1"
                     /translation="MAGFALKPALQLWWKAVRPYAYSASLVPVAIGGFYARFHGGDWS
                     PVRFGIALLAGLLVHTAANLWNDYYDFKSGVDHTGGGVGSGVLVAGEMSPAVCFRGAV
                     VCAALSGLLGIWLITQVGWALLWLGLFGIAGAWAYSAGPFSPKHRALGEVWVFLLMGA
                     GMTLGGYLAQTGCFAWRAVGVGVPAALLMTLILYVNNLRDIRSDRAAGLHTLPMLLKP
                     LTAKLVVALVLLGAYGLASAMAIGRHVPLATLAVWLAGPLAWRLFRQTWRGPVREQEV
                     VGAAMLHMAFGLLFAVGLWFR"
     gene            complement(2191..2760)
                     /locus_tag="PKI64_09155"
     CDS             complement(2191..2760)
                     /locus_tag="PKI64_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01206.1"
                     /translation="MPMNDQHIFRNRREKHSGALRRPWVSGVVLLTLALVTGLWLYSH
                     FQPEARVRRASTKLAVLVQKQEEESDMALALAANRLSQQLEPQAKLSVEGYGIALAER
                     TEIVQLFAQLRRTFARIELSGSRVAARQQPNGEILAQVVAKYRLVYHGEEKAGNGQAD
                     LWWRKTNQGWRIRHVEIYLENTESWPALP"
     gene            2807..3337
                     /locus_tag="PKI64_09160"
     CDS             2807..3337
                     /locus_tag="PKI64_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOE01207.1"
                     /translation="MSDQPTMQEYIHSAQEILSNLLRLSDFGDVQVETTVADGQIFFQ
                     LVSEDTGRLIGRTSQTLDALQFLLNRLLSRQYDISPYCVVDAGHYRERRREKLLADAQ
                     AALQRVRQTGAPWRMPPLSSMERRIIHQALKDLPDIRTQSEDMDSEGRKRVVISLAED
                     FQPGQPAEDAPAPSPE"
     gene            3402..4826
                     /locus_tag="PKI64_09165"
     CDS             3402..4826
                     /locus_tag="PKI64_09165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE01208.1"
                     /translation="MKHILIPFLLLSLLGWLGAGCGKKDAPVAVEPAAQVPDTQPALP
                     DLTAALVDAAEAVEVVEPELTAEEAAVVIAKVGGKDITEGDIQKILRAFKKQMQGRVM
                     PGQWEEALPKIRGRILDDLIMQQILAAEVLRQGVSLSESEYQEIKNEYAAELPPGMTF
                     ETYLAETGTTEANLREQIAIRKMLTAKGNAVEKPTDEEIQAFYEENKEGFTQGETVTA
                     SHILVKVDPDETGDAKEAKRARIAELRQQLLDGADFAEVAKANSDCPSASAGGDLGSF
                     GRGQMVAPFEDAAFAQPVGSVGDIVETQFGFHLIKVTAHDKAQTLDLNEVKPRISDIL
                     YAQKQQEVVMAYLEGLREQADVQRFDEEPAGDEAAFTLDVEEEATEAAPADEPAEDAA
                     PADTPSTVVDEAVQAADDAVEAVTEAVEEAAETAAETVVETGKKAKKAGKKAAAAVVE
                     EATEAVDAVKEAIAPAETEEPAAE"
     gene            complement(5248..5922)
                     /locus_tag="PKI64_09170"
     CDS             complement(5248..5922)
                     /locus_tag="PKI64_09170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE01209.1"
                     /translation="MNPPPESAARQPFFVHPSSEVAPTALVGEGTKIWHFCHVMKEAK
                     IGRGCNLGQNVHVASGVLIGNNVKIQNNVSLYTGATIEDDVFLGPSCVFTNVTNPRSQ
                     VNRHSLYEKTLVRRGATVGANATIICGVTLGRYCFVAAGSVVTKDVPDYALVVGNPAR
                     QKGWMSRHGHLLKNANAEGIMVCPESGFRYRKTPEGILRCLDCDEDAPLPPNLAKGTK
                     FYDEFK"
     gene            complement(5954..6904)
                     /locus_tag="PKI64_09175"
     CDS             complement(5954..6904)
                     /locus_tag="PKI64_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE01210.1"
                     /translation="MKKIALVGCGRIMGRHIEAIATAPGLEIAMVCDIDEAKARKTAE
                     RLGVPYVTDYRRIQGVDVAAILTPSGLHPQHVTDIAEQTDTPYILCEKPISLTLREAY
                     ELFDRVDRAGKTFLPIYQNRYNPLVAFTKNLIESGRLGQVHLFVCNVLWNRNDDYFKI
                     DWHGTRALDGGVLYTQASHYVDMVHYFFGDVVAHKGIGGSLRGLEVFDSVAAAMSFKN
                     GTVGTINATVSTFRENYRTEFTLIGQRGTLRLAGTNLNTIEFWDVEGMAKPDIDFTID
                     HVYGKGHDTLYQYLAAGRWDMFPTRKDVLSGIRLMEMLSY"
     gene            7122..>8035
                     /locus_tag="PKI64_09180"
     CDS             7122..>8035
                     /locus_tag="PKI64_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE01211.1"
                     /translation="MIQNKKIFITGGAGFIGSTLIGRLVEHNQIVAFDNLARNALQTK
                     PWKDHKNLKLEVGDVLDYDHVARAMAGADIVVHCAAIAGIDTVVKNPVATMRVNMVGS
                     ANVLEAASKLDSCDRVVCFSTSEVFGTNAFRSSEMDKTSIGTIGEGRWTYAVSKLAEE
                     HLAIAYFQHQWLPVAVVRPFNVYGPGQVGEGALRAFIQRALKNEPLKINGDGTQIRAW
                     CYVDDMVRGTLLCMEHPKAVGESFNIGNQRAVTTIYGLANTVIRVLNSKSKIEFVYKD
                     YADVELRIPNVKKARDLIGFEAEVDLEEG"
BASE COUNT         1750 a         2550 c         2300 g         1435 t
ORIGIN      
        1 acccacggta aaaatcacga gccaacagcc ccggcctcac cgaggccggc tacagtaaag
       61 ccgtgctggg ccttgtccgc tgtgcgcaac atgtggctgt tgcctgaact ctgaaccctg
      121 ctacctgacc tctctgccga gcgtcgcttg cgttaaaatc cttctacttg gcggcagcca
      181 gcagaaagtg gcgcaagacc ttgcgtgcgg tttccagcac ggcagtttcg atgtattcat
      241 ccacggtatg agcttgggcg atggcgccgg gaccccagac gatggtttgg ccgcaggcaa
      301 gcgccaaggg gccagcatcg ctgcaccacg gggcgccggt gaccacactg ggcaccccac
      361 cggccgccag agcagcggaa aactcccgaa cgagcggcgc atcggcgggc gtatggaagg
      421 gcaggccttc cttcaacaag gtcagctcca ccccgccggg cacgactgcc aagcgttgct
      481 gcaaatcagc aaaaacagcg ggcgcgcttt cgccgggcat tagccgccgg tccacttgga
      541 tttcgcaacg gtcgggcaca atgttggtgc cgctgccgcc ttgaatagtg ccgatggaga
      601 tggtgggcga gcccagcacg tgtccgggat attgcttggc agcttcggcg gtggcctcgg
      661 ccaagatgcc ccacacctgc gccattttca ggatggcgtt atcgccgcgg gaagggtcgg
      721 aagcgtgagc cgcgcggccg cgggtggcgg ctgtgaacca acaaacccct ttgtgggcat
      781 ggaccacttg caaatcggtg ggctccagca ccacgcattc atcggcacgc agcccggcgg
      841 caatggcttc ttccgcgcca aggttgccgg tttcttcgcc gatggcaccc agccaaatca
      901 gttcgatgga cgatgtcgcg agtgcggcga gcacctcatc atccagcgtg gccataaagg
      961 cggccagcgg ccctttggtg tcgcacgcac cgcggccata aaggcgcccg ttttcttccc
     1021 gcagggtgaa ggggtcgcct tgaaagccgg ccacgccgac cgtgtccaag tgcgactcaa
     1081 acagcagggt gcgccggggt tggcgcgcgc caacgcggac cagcagattg gggcgaccgg
     1141 gcgtttgccc cacgcgttcc acgcgaaagc ctcgcgcggc aaaccaaggc tccagccaat
     1201 ccaccaagcg ggcttcgttg cgttgggcat cgtcggtgga catggccgga ttcacgctgg
     1261 ggagcgcgac gagttgacga agccattcaa tggactgcat tttcatgatg gcagtttagc
     1321 ggaaccaaag gccaacggca aacagaagcc cgaacgccat gtggagcatg gcggcgccca
     1381 cgacttcctg ctcgcgcacg ggcccccgcc atgtttggcg gaacaagcgc cacgccagcg
     1441 gcccggcgag ccaaaccgcc agcgtggcta gcggtacatg ccggccaatg gccatcgcgc
     1501 tggccaagcc ataggccccc agcagcacca gtgccaccac cagcttggca gtcagcggct
     1561 tgagcaacat cggcaacgta tgcagcccgg cggcgcggtc agagcggata tcgcgcagat
     1621 tattcacgta gaggatcagc gtcatgagca gcgccgcggg cacgcccacc cccaccgcgc
     1681 gccacgcgaa gcagccagtt tgcgcgagat agccccccaa ggtcattccg gcgcccatta
     1741 acaggaagac ccacacctcg cccagcgccc gatgcttcgg gctgaacggc ccggcggagt
     1801 aggcccacgc ccccgcgatg ccgaacaacc ccagccacag cagcgcccaa cccacttgcg
     1861 tgattagcca aatgcccagc aagccactca acgccgcgca aaccacggca ccccggaaac
     1921 aaaccgctgg cgacatctcg cccgccacca gcacgccgct gcccactccg cccccggtgt
     1981 ggtccacccc gcttttgaaa tcataataat cgttccacag attggcggcg gtgtgcacca
     2041 gcaagccggc cagcagggca atcccgaagc gaactggcga ccagtcgccg ccgtggaatc
     2101 gggcatagaa cccgccaatg gccacgggaa ccaagctggc cgagtaggcg tagggccgca
     2161 cggctttcca ccacagttgc agcgcgggtt tcagggcaaa gccggccatg actcggtatt
     2221 ctccaaatag atctccacat ggcggatacg ccagccttga ttggttttgc gccaccacaa
     2281 atcggcttgg ccattgccgg ccttttcctc gccgtggtac accagccgat acttcgccac
     2341 gacttgcgcc aggatttcgc catttggctg ttggcgcgca gccacgcgcg atccgctgag
     2401 ttcaatgcgc gcgaaggtgc gccgcaactg ggcaaaaagc tgcacaattt ccgtgcgttc
     2461 agccaaagct attccgtacc cctccacgct gagcttggcc tgcggctcaa gctgttggct
     2521 caaccgattg gccgccaacg ccaaggccat atctgattct tcctcctgct tctgaaccag
     2581 cacggccagt ttggtcgaag cgcggcgcac ccgggcttca ggctggaaat ggctgtagag
     2641 ccaaagcccc gtcaccagcg ccaaagtcag cagtaccacg ccggacaccc acggtcgccg
     2701 caacgcgccg ctgtgctttt ctcggcgatt tcggaaaata tgctggtcat tcatcggcat
     2761 tcagcataca aaatctccct aaattggaaa tgcggaaaga agacccatga gcgaccagcc
     2821 aacgatgcag gaatatatcc attccgccca agaaatcctc tccaacctgc tgcgcttgtc
     2881 ggattttggc gatgtgcaag tggaaaccac cgtggccgat ggccagattt tttttcaact
     2941 ggtgagcgag gataccgggc ggctgattgg ccgcacatcg caaacgctgg atgccctgca
     3001 atttctcctg aaccgcctgc tgtcgcgtca atatgacatc tcgccctact gcgtggtgga
     3061 tgcaggccat taccgcgagc gccgccgcga aaaattgttg gcggatgctc aagccgcgct
     3121 acagcgcgtc cggcaaacgg gcgccccgtg gcggatgccc cctctcagct ccatggagcg
     3181 gcgcatcatc catcaggcgc tcaaagattt gccggacatc cgcacccagt ccgaggacat
     3241 ggacagcgaa ggccgcaagc gggtcgtgat ttccctcgcg gaagacttcc agcccgggca
     3301 gccggctgag gatgcccccg cccccagccc ggaatgaccc ggccatgctt ttggtcactg
     3361 gtaatccgcc ccctaacgtc aaaacccgaa aggaccgccc catgaaacat atcctgatcc
     3421 cgtttttgct cctgagtctc ctcggctggc tcggtgctgg ctgcggcaaa aaagacgccc
     3481 cggttgccgt tgaaccggcc gcccaggttc ccgacactca acccgcgctg ccggacctca
     3541 ccgccgcgct ggtggatgct gccgaagccg tcgaagtggt cgaacccgaa ctgaccgccg
     3601 aagaagccgc cgtggtcatc gccaaagttg gtggcaagga tatcaccgaa ggtgacattc
     3661 aaaagatttt gcgcgcgttc aaaaagcaga tgcaaggccg tgtgatgccc ggccaatggg
     3721 aagaagccct gccgaaaatc cgcggccgga ttctcgacga cctgatcatg cagcagatcc
     3781 tcgcggccga agtgctccgg caaggggtca gcctttcgga aagcgaatat caggaaatca
     3841 aaaatgaata tgccgcggaa ttgccgcctg gcatgacttt tgaaacctat ctggccgaaa
     3901 ctggcaccac tgaggccaac ctgcgcgaac aaatcgccat ccgcaaaatg ctcacggcca
     3961 agggcaacgc ggtcgagaaa cccaccgacg aagaaatcca ggccttttat gaggaaaaca
     4021 aggaaggctt cacccagggc gaaaccgtca ccgcctccca tattttggtc aaggtggatc
     4081 ccgacgaaac cggcgacgcc aaggaagcca agcgcgcgcg catcgcagaa ttgcggcaac
     4141 aactgctaga cggcgcggac ttcgcggaag tggccaaggc caactccgac tgccccagcg
     4201 ccagcgccgg tggcgatctc ggctcctttg gccgcggcca gatggtggcc ccctttgaag
     4261 atgcggcgtt tgcccagcca gtcggctccg tgggcgatat tgtcgagacc cagttcggct
     4321 tccacctgat caaagtcacc gcacacgaca aagcccagac gctggatctc aatgaggtca
     4381 agccgcgcat cagcgatatc ctgtacgcgc aaaaacagca ggaggttgtg atggcctact
     4441 tggaaggcct gcgcgaacaa gccgatgtgc agcgctttga cgaagaaccg gccggtgacg
     4501 aagccgcctt cactctggat gttgaagaag aagccaccga ggcagccccg gcggatgagc
     4561 ccgcggagga cgcggccccg gcggataccc cctccactgt ggtggacgaa gccgtgcaag
     4621 ccgctgacga tgccgtcgaa gccgtgacgg aggctgtgga agaagccgcc gaaaccgccg
     4681 cggaaacggt tgtcgaaacc gggaaaaaag ccaagaaagc cggcaaaaaa gccgccgcgg
     4741 ccgttgtgga agaagccacc gaggccgtgg acgcggtgaa agaagccatt gccccggcag
     4801 aaaccgaaga acctgctgcg gaatagccac cagcgcgcga cgaagcccgg gcccccgccc
     4861 gggctttttt tctgacttcc gcaatgggac acccaacaat ttcgctgatt ttgtcgggct
     4921 tttgtagggc gggaacggtt gaggaacgaa accttcccgc cgggaaaggc tggaaggtct
     4981 gcgaccattc cagccccaca atccaaaccg gaatcagttt cttgggccgg gacaccccaa
     5041 aacgaaatcg tccggttgca atacaacgac ttacaatgtg attgttaaaa ggtactcgga
     5101 actcaagcca ttgccccggc agaagccgaa gaacccgcgg cggaataact gccagcgtgc
     5161 gacgaagccc gggcccccgc ccgggctttt tgtgcccccc cccccagcct gccacctcac
     5221 cctgccggac gcaggcgttg gcgggagcta tttgaattca tcgtaaaatt tggtgccctt
     5281 ggccaagttt ggcggcaggg gcgcatcttc gtcgcaatcc aggcagcgca aaatgccctc
     5341 tggtgttttc cgataacgaa aacccgattc aggacaaacc atgatgccct cggcattggc
     5401 atttttgagc aggtggccat ggcggctcat ccatcctttt tgccgcgccg gattgccaac
     5461 caccaaggca tagtcgggga catctttggt caccacggag ccggccgcaa cgaagcaata
     5521 gcgcccgagg gtcacgccac aaatgatggt ggcattggcg ccgacggttg ccccgcggcg
     5581 aaccagcgtt ttttcgtaca ggctgtgccg gttgacctgg ctccggggat tggtcacatt
     5641 ggtgaaaaca caggaagggc cgaggaatac atcgtcttcg atggtggccc cggtatagag
     5701 cgacacgttg ttttggattt tgacgttgtt gccaatcagc acgccggagg ccacatgcac
     5761 attctggccg agattgcacc cgcggccaat cttggcctcc ttcattacat ggcaaaaatg
     5821 ccagattttg gtgccctcgc ccactagcgc ggtgggggcc acctccgaag acggatggac
     5881 aaaaaagggc tggcgcgcgg cggattcagg gggaggattc atggcgggac tccggacggg
     5941 ggcagaacat ggctcaataa ctcaacattt ccatcagccg gatccccgac aacacatcct
     6001 tgcgggtggg gaacatatcc cagcgcccgg cggcgagata ttggtagagg gtgtcatgac
     6061 ctttgccata aacgtggtca atggtgaagt caatgtcggg cttggccatc ccctccacat
     6121 cccagaattc gatggtattg aggtttgtgc cggcgaggcg caaggtgccc cgctggccaa
     6181 tgagcgtgaa ttccgtgcgg taattctcgc ggaatgtgct caccgtggca ttgatggtgc
     6241 ccaccgtgcc attcttgaag ctcatggccg ccgcaaccga atcaaacacc tccagccccc
     6301 gcaagctgcc cccgatcccc ttgtgcgcaa ccacgtcccc aaagaaatag tgcaccatgt
     6361 ccacatagtg gctcgcctgg gtgtagagca ccccaccatc gagcgcccga gtgccatgcc
     6421 agtcaatttt gaaataatcg tcgttgcggt tccacagcac gttgcataca aacaggtgca
     6481 cctggcccaa gcgcccgctt tcaattaggt ttttcgtgaa ggctaccagc gggttgtagc
     6541 ggttttgata aatcggcaaa aaagttttgc cggcccggtc cacgcggtca aaaagctcgt
     6601 aggcctcgcg cagcgtcagg gaaatcggct tttcgcagag gatgtagggg gtgtcggttt
     6661 gctcggcaat atccgtcacg tgttggggat gaaggccgga aggcgtcaaa atggccgcga
     6721 catccacacc ttgaatgcgc cggtaatcgg tcacataagg cacgccgagt cgctccgcgg
     6781 tttttctcgc ttttgcctcg tcaatatcgc aaaccatggc aatttcgagc cccggcgccg
     6841 tcgcaatcgc ctcgatatgc cggcccatga tccgcccaca gccaaccaac gcaatttttt
     6901 tcatatcagt ttgcattcca cttcgagtgc cgcgcatcct accaccccac tgcccccctg
     6961 tgcattcaaa atattggccg gatattgagg caggaaacca cgccatgccg gcgacgcttg
     7021 cgtcaacaaa aggcttgctg ctatccgcac gcctgcacca tactgcccgc tcatctcaat
     7081 aaggcaaatg gtggcctaac cgccaccggc gaggaccaac catgatccaa aacaagaaga
     7141 tttttatcac cggcggcgcg gggtttatcg gctccaccct gattggccgc ttggttgaac
     7201 acaatcagat cgtagccttt gacaatctgg cccgtaatgc gctccagacc aagccgtgga
     7261 aagaccacaa aaacctcaag ctggaagtcg gcgatgtcct cgactatgac cacgttgccc
     7321 gggccatggc gggggccgat atcgtcgtcc attgtgcggc gattgccggg attgacaccg
     7381 tcgtcaaaaa cccggtggcc accatgcgcg taaacatggt gggctccgcg aatgtcctgg
     7441 aagccgcctc caagctggat agctgcgacc gggtcgtgtg cttttccacc agcgaagtgt
     7501 tcggcaccaa cgccttccgc tcctcggaaa tggataagac ctccattggc accattggcg
     7561 agggccgctg gacctacgcc gtcagcaaat tggccgagga acacctcgcc atcgcctatt
     7621 tccaacacca atggctcccg gttgccgtgg tgcgcccatt caatgtctat ggcccgggtc
     7681 aggtgggtga aggcgcgcta cgcgctttca ttcagcgtgc tttgaagaat gagcctctca
     7741 agatcaacgg cgacggcacc caaatccgcg cttggtgcta cgtggacgac atggtccgcg
     7801 gtaccctgct ctgcatggaa caccccaagg ctgttggcga atcgttcaat atcggcaacc
     7861 aacgtgccgt caccaccatt tacggtctgg ccaacacggt tatccgcgtc ctgaactcca
     7921 agtccaagat cgaattcgtc tataaagact acgccgacgt cgaactccgc atccctaacg
     7981 tcaaaaaagc ccgcgacctc atcggcttcg aagccgaagt ggatttggaa gaggg
//
LOCUS       DAOPNP010000111         7962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_10157_length_7962_cov_9.158973, whole genome shotgun sequence.
ACCESSION   DAOPNP010000111 DAOPNP010000000
VERSION     DAOPNP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10157_length_7962_cov_9.158973"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..86)
                     /locus_tag="PKI64_09185"
     tRNA            complement(2..86)
                     /locus_tag="PKI64_09185"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Tyr,seq:gta)
     gene            complement(111..186)
                     /locus_tag="PKI64_09190"
     tRNA            complement(111..186)
                     /locus_tag="PKI64_09190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Ala,seq:cgc)
     gene            complement(260..1822)
                     /locus_tag="PKI64_09195"
     CDS             complement(260..1822)
                     /locus_tag="PKI64_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE01212.1"
                     /translation="MNFFERVKAVFGGAKTRHPANVPANVPRPAKPHGEKKAAPGTRF
                     EEAPRSAKTAEPEVWRAGSRKKRDKPPHHHPRKSGRGGAEPASATPWDRREYQVAPAE
                     DKTRFLDLDLPDVLLRALADLDFRYCTPIQAKALPVALNGQDVCGRAQTGTGKTAAFL
                     LTIFKYLLDHPAPPQRRKGFPRALIMAPTRELCLQIAKDGEALGKYTGLRLLAVYGGM
                     DLQAQRAVLRDKHVDIVVATPGRLLDFRQRQDVDFSKVEILVIDEADRMLDMGFIPDM
                     RRIVYGCPPKDERQTLLFSATLTPDVTRLAAQWMRNAVTVDIAPEQVAAETIDQRVYI
                     VTEDQKFTIVYNILKDLAPERVIIFGNRRDSTELLCANLRHQGVDVALLSGAVEQRKR
                     LAVLENFRSGRVPVLVATDVAGRGLHIDGIDLIINYNIPEQAEDYVHRIGRTGRVGEK
                     GTSITFADEIDSYYIPAVEEYMKRPLKCIPPPDEWLAPLPKPLIEKKRRSRPADARGN
                     RPRGPRRPPRRR"
     gene            complement(1857..3542)
                     /gene="rpiB"
                     /locus_tag="PKI64_09200"
     CDS             complement(1857..3542)
                     /gene="rpiB"
                     /locus_tag="PKI64_09200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOE01213.1"
                     /translation="MKIGLGADHGGFEMKQKIKDLLAKRPEVEVTDFGTYSPESVDYP
                     DFAIEVARRVSEGTLEQGILICTTGVGMSMTANKFPRVRAAQVFNAKMARMTREHNNA
                     NIMALGAAITPMEEIPAILDAWFSASFEPGSRHDRRVQKINACAMRVTEPEAIYEHDT
                     EIYAAVQGETKRQRQNIELIASENYVSRAVREAQGSVLTNKYAEGYPGKRYYNGCEFV
                     DEAERLAIERAKQLFGAEHVNVQPHSGSGANMAVYFAMMQPGDTMLAMGLAHGGHLTH
                     GHKVNFSGRFFNVVSYGVDPKTERIDYEQVEALAREHKPRMICAGASAYSRIIDFKRL
                     RAIANDVGALLMVDMAHIAGLVATGFHPSPIPYADFVTTTTHKTLRGPRGGMVLCKEA
                     YAADIDKQVFPGIQGGPLMHVIAAKAVCFLEALQPAYKEYVRQVVINAQTLAAELEKA
                     GLRIVSGGTDNHLMLADLTPLGVTGKDAATALDHALITVNKNAIPFDKQKPFVTSGIR
                     LGTPAVTSRGMKEADMAQIAAWIGAIVAKPGDEKLQQRIRGEVAAFSEKFLVP"
     gene            3850..4128
                     /locus_tag="PKI64_09205"
     CDS             3850..4128
                     /locus_tag="PKI64_09205"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOE01214.1"
                     /translation="MKKLAVFLAALFVLSAAAWALDIEMQAVESSLIAKVGYSADAKE
                     LVVQMHNSSDIYVYKGVPQAVYDDFLAADSKGAFYVKNIKGQYETERK"
     gene            complement(4531..5457)
                     /locus_tag="PKI64_09210"
     CDS             complement(4531..5457)
                     /locus_tag="PKI64_09210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE01215.1"
                     /translation="MSANLQTLANLVGTSREMQEVRDQIVCHAAESTPLLVVGEPGTG
                     RVAVAQAVHGASQRQDRPFQQVYCAALPAAALARQLFAHANDGPADKPGLLQLAAGGS
                     LLLAGADDLPTTLQLQLLTALRGEPPADVRLLLTTEHDAPQLAAEGRFHPDWAAWLAP
                     NTIYLPPLRKRKTDIVLLADHFLPHYAQALGKDVRRLASATIDMLMSYHWPGNVRELE
                     NSMARAVQQAEGSVIYPYHLPPTLQTAEAGAMPVAGDLKSLVSAFERDLIRDALKSTR
                     GNVAAAARALGTTPRILGYKIRTYQIPPRPYD"
     gene            complement(5482..7656)
                     /locus_tag="PKI64_09215"
     CDS             complement(5482..7656)
                     /locus_tag="PKI64_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOE01216.1"
                     /translation="MSNARLNAIAAVTNYRATAPALDFSKTSAGEIFGSNVFNDRVMK
                     ARLSPAVYEALRRTIERGEKLDVTVADEVAAAMRDWAIEKGATHYAHVFYPLTGLTAE
                     KHDSFLEPDGKGGAIAAFSAKQLIRGEPDASSFPSGGIRATFEARGYTAWDVTSPAYI
                     LENPNGTALCIPTAFCSWTGEALDNKTPLLRSMQALSAQAQRLLRIFGEENPELVYAT
                     AGCEQEYFLVDRNFFFARPDLISAGRTLFGAQPPKGQEMEDQYFGAIPERVLAFMLEV
                     ERELYKLGVPVKTRHNEVAPGQYELAPVFEQANVAADHQHLTMTVLQRVAQKYGMSCL
                     LHEKPFARVSGSGKHLNWSLGTRHSNLLEPGDTPERNMRFLAFCAAMIRAVDKHSTLL
                     RASVAHAGNDLRLGANEAPPPTLSIFLGDQLTDVFEGIARGGSGRARKSGVLTIGVDT
                     LPPLPKDAGDRNRTSPFAFTGNKFEFRTVGSSQSVAGPLIVLNTIMADSLDYIATKLE
                     AAGAKTPEEIGAALQGVIRKIMKDHGRVIFNGDNYSPAWHEEAAARGLPYAPYAAAAI
                     PAAQTLANSEVLARFNVLSPREFASRNEIYLEKYIKEVMIEARLTFEIAKTMIFPATV
                     TYQKLLAETSLMLKQMGKEHCTTVLDEVCELAAQLQTRIATLRTAIAHEAKGDIPAHA
                     RFCREHIVPAMDAVRAVADQLENIVADEHWPLPTYQEMLFIK"
BASE COUNT         1415 a         2224 c         2681 g         1642 t
ORIGIN      
        1 atggtggtgg gggatggatt cgaaccatcg aactcataga gggcagattt acagtctgct
       61 gcggttggcc acttcgctac cccaccgggg attcaggtta tgcgcgcact tggtggagca
      121 gaagggattc gaaccctcga cccctacgtt gcgaacgtaa tgctctacca actgagctac
      181 tgccccgagt ggcgcatatc cttggcaatc gtcgagcgcg caacctacca gccaacagca
      241 tctggcgcaa gggttttttc tagcggcggc gcgggggccg gcgcggtccg cgcgggcggt
      301 tgccgcgggc atcggcaggg cgggagcgcc gttttttttc gatgagcggt ttgggcagcg
      361 gcgccagcca ttcatcgggc ggcggaatgc acttgagggg ccgcttcatg tattcttcga
      421 cggcgggaat gtagtagctg tcaatctcgt cagcaaaggt gatggaggtg cctttttcgc
      481 cgacgcggcc ggtgcggcca atgcggtgga cgtagtcttc ggcttgttcg gggatgttgt
      541 agttgatgat cagatcaatg ccatcaatgt gcaggccgcg accggcgaca tcggtggcga
      601 ccaaaaccgg aacacggccg ctacggaagt tttccagcac ggcgaggcgc ttgcgttgtt
      661 ccaccgcgcc ggagagcagc gcgacatcca cgccctgatg gcgcaggttg gcgcacagca
      721 gctccgtgga gtcgcggcgg ttgccgaaaa taatgacgcg ctcgggcgca agatccttga
      781 ggatgttgta cacgatggtg aatttttggt cttccgtgac gatgtacacg cgctggtcaa
      841 tggtctccgc ggcgacctgc tcgggggcga tatccacggt gacggcattg cgcatccatt
      901 gagcggccag ccgggtgaca tccggggtaa gggtggcgct gaagagcagg gtttggcgct
      961 cgtccttggg cgggcagcca tacacgatgc ggcgcatgtc ggggatgaag cccatgtcga
     1021 gcatccggtc agcctcgtca atcacgagga tttcaacctt ggaaaaatcc acatcctgcc
     1081 gctggcgaaa atcgagcagg cggccggggg tggccacgac gatatccacg tgtttatcgc
     1141 gcaggacggc gcgttgggct tgcaggtcca tgccgccata gacagccagc aggcgcaagc
     1201 cagtatattt gcccagtgcc tcaccatctt tggcgatttg caggcatagt tcgcgcgtgg
     1261 gggccatgat gagcgcccgg ggaaagccct tgcggcgttg cggcggcgca gggtgatcca
     1321 gcaaatactt gaagatggtc agcaaaaaag cggcggtttt gccggtgccg gtttgtgccc
     1381 gcccgcagac atcctggcca ttgagggcca cgggcagggc cttggcctga atgggggtgc
     1441 aatagcggaa gtccagatcg gcgagggcgc gcagcagcac gtcgggcaaa tccaaatcca
     1501 agaagcgggt tttgtcttcg gcgggggcca cctgatattc gcgccggtcc catggcgtgg
     1561 cgctggcggg ctccgcgccg ccgcggccgg atttgcgcgg gtggtggtgg gggggcttgt
     1621 cgcgtttttt gcgggagccg gcgcgccaga cttcgggctc ggcggttttt gcggatcgcg
     1681 gggcttcttc gaagcgtgtg cccggggcgg cttttttctc gccatggggt ttggcggggc
     1741 ggggtacgtt ggcgggcacg ttggcaggat gccgggtctt ggcaccgcca aaaacggctt
     1801 tgacgcgctc aaaaaaattc atggcgggat atttcggggt tcgtggtggg cgcggctcaa
     1861 ggcacgagga acttttcgga gaaagcagcc acttcgccac ggatccgctg ctggagtttt
     1921 tcgtcgccgg gcttggcgac aatggcgcca atccaagcgg cgatttgggc catgtcggcc
     1981 tccttcatgc cgcgggaggt cacggcgggg gtgccgaggc ggatgccgga agtgacaaac
     2041 ggcttttgct tgtcaaacgg gatcgcgttt ttattgacgg tgatcagcgc gtggtcgagc
     2101 gcagtggcgg cgtccttgcc ggtgacgcca aggggggtga ggtccgccag catcaggtgg
     2161 ttgtcggtgc caccggagac gatgcgcagc ccggcttttt ccagttcggc ggcgagggtc
     2221 tgggcgttga tgacgacttg gcgcacatat tccttgtagg ccggctggag ggcttcgagg
     2281 aagcaaacgg ctttggcggc aatgacgtgc atgagcggcc cgccctggat gccggggaac
     2341 acttgcttgt caatgtcggc agcgtaagcc tccttgcaca acaccatgcc gccgcgcggc
     2401 ccgcgcaggg tcttgtgggt ggtggtggtg acaaagtccg cgtaggggat ggggctggga
     2461 tggaagccgg tggccaccaa gccggcaatg tgcgccatgt ccaccatcag cagtgcgccg
     2521 acatcgttgg cgatggcccg caggcgcttg aagtcaataa tgcgggaata ggcgctggcg
     2581 ccggcgcaaa tcatgcgcgg cttgtgctcg cgagcgagcg cctcgacctg ctcgtaatca
     2641 atgcgctcgg tcttggggtc cacgccgtag gacaccacat tgaaaaagcg tccagagaag
     2701 ttgaccttgt ggccgtgggt caggtggccg ccgtgggcca agcccatggc gagcatggta
     2761 tcgcccggct gcatcatggc aaaatagacg gccatgttgg cgccgctgcc gctgtgcggc
     2821 tgcacgttca cgtgctccgc gccgaataac tgcttggcgc gttcgatggc cagccgctcg
     2881 gcctcatcca caaactcgca gccgttgtag tagcgcttgc cgggatagcc ttcggcatat
     2941 ttgttggtca gcaccgagcc ttgcgcctcg cggacggcgc ggctgacgta attttcggag
     3001 gcaatcaact cgatgttttg ccgctggcgc ttggtttcgc cctgaaccgc cgcatagatt
     3061 tcggtgtcgt gctcgtagat ggcttcgggt tccgtgacgc gcatggcgca ggcgttgatt
     3121 ttctggacgc ggcggtcatg gcgcgagccg ggttcaaaag aggcgctgaa ccaggcatcg
     3181 aggatggcgg ggatttcttc catgggtgtg atcgccgcgc ccaacgccat gatgttggcg
     3241 ttgttgtgtt cgcgggtcat gcgggccatt ttggcgttga acacctgggc tgcacgcacg
     3301 cgggggaatt tgttggccgt catgctcatg cccacgccgg tggtgcaaat caggatgccc
     3361 tgttcgaggg tgccttcgga gacgcggcgg gccacttcga tggcaaaatc gggataatcc
     3421 acggattcgg ggctataggt gccgaagtcg gtcacctcca cctccggccg tttggccagc
     3481 aagtccttta ttttttgctt catttcgaag ccgccgtggt ctgcaccaag tccgattttc
     3541 atgaaaaaac ccttgttatt ggcattttgt cgctctggtt agccggcaac gggccggccc
     3601 atcggcccgg ccggcggggt gttgttcccc ggcgcggcgg cctgtccaag aatcaggcgt
     3661 ttacgaatac cccagcccac ctgatactgc aaggctggaa aacgcgttgg cggagcaggg
     3721 gcacgggaca acttcgcccc ccagggggca ctaaataccc tttttggggc atcaaacgga
     3781 cgggaaaggc agcttgattt caccggtcgc ctgaagtaaa ttggggcgca taacaaggag
     3841 tgttttgcca tgaagaaact agccgtattt cttgccgctt tgtttgttct ttctgctgct
     3901 gcgtgggcgc tggatattga aatgcaggcg gtggaatcct cgctgatagc caaggtgggc
     3961 tactctgccg acgccaagga attggtcgtg caaatgcaca acagctccga tatctatgtt
     4021 tacaaagggg tgccgcaggc ggtgtatgac gacttccttg ccgccgattc caagggcgcg
     4081 ttttacgtta agaacatcaa ggggcaatac gagacggaac ggaagtaaac gatccgtcgc
     4141 gccgagcgct gcaccatgtg tgcggcgctt tttttacgcc gccgtttcgt gcgcgctcgg
     4201 cggaggcttg gttgggagga tttcttgcag attttggcgg cgccaaaacc aaccggcatc
     4261 ttttccgcgc tggcgcgccg cgaccgaccg ctcgcggaca cgagtccagc tcgggtcgtc
     4321 cgcaacgcgc catcatcgga aaatccgccg cttattttgg cgcagcagcc ccgccaaaat
     4381 ccgcaagaaa tcctccgcat gcacgtgctg cgcacagcgg acaagatttg caggccgggg
     4441 ttgtcatgct tggcggtttt tttgcctctt ccctttgctt cctttggcgt cgcttgcgtt
     4501 aaaaggctgc tcggctgttc ggccggcggg tcagtcgtag ggccggggcg ggatttggta
     4561 ggtgcggatt ttatagccga ggatgcgcgg cgtggtgccc aacgcgcgtg cggcagcagc
     4621 cacattgccg cgcgtggatt tcagcgcatc gcggatcaag tcgcgctcga acgcgctcac
     4681 gagcgattta agatcgccgg ccaccggcat ggcgccggct tcggcggttt gcagcgtggg
     4741 cggcaggtgg tagggataaa tcacactgcc ttcggcctgt tgcacagcgc gggccatgct
     4801 gttttccagt tcgcggacgt tgccgggcca atgatagctc atcagcatgt caatggtggc
     4861 gctggcgagg cggcgcacat ctttgccgag cgcttgggcg tagtgcggca aaaagtgatc
     4921 ggccagcagc acgatatcgg ttttgcgttt gcgcagcggc ggcaaataga tcgtgttcgg
     4981 cgcgagccac gcggcccaat ccggatggaa gcgcccttcg gccgccagtt gaggcgcatc
     5041 gtgctcggtg gtgagcagca ggcgcacgtc ggcgggtggc tcaccgcgca aggccgtgag
     5101 caactggagt tggagggtcg ttggcaaatc atccgcgccg gccagcagca gcgagccgcc
     5161 cgccgccaac tggagcaggc cgggcttgtc ggcgggacca tcgttggcgt gcgcgaacaa
     5221 ctggcgcgcc aacgccgccg ccggcagtgc ggcgcaatat acctgctgaa agggtcggtc
     5281 ctgtcgctgg ctggcgccat gcaccgcctg cgctacggca acccggcccg tgcctggctc
     5341 gcctaccacc agcagcggcg tggattcggc tgcatggcag acaatttggt cgcgcacctc
     5401 ctgcatttcg cgggaggtgc caaccagatt cgcgagggtt tgcagattcg cactcatggc
     5461 ttaccggcga cgcgggccgc cttacttgat gaacagcatc tcctgatagg tcggcagggg
     5521 ccaatgttcg tcggccacga tattttcgag ttggtcggca acggcgcgca cggcatccat
     5581 cgcgggcacg atgtgctcgc ggcagaagcg ggcatgagcg gggatgtcac ccttggcttc
     5641 gtgggcgatg gctgtgcgta gcgtggcaat ccgcgtttgg agttgggcgg ccagctcgca
     5701 gacttcgtcc aggaccgtgg tgcaatgttc cttgcccatt tgcttgagca tcagggaggt
     5761 ttccgccagc aacttctggt aggtgacggt ggcagggaaa atcatggttt tggcgatctc
     5821 gaacgtgagg cgcgcctcaa tcatgacttc cttgatgtat ttctccagat agatttcgtt
     5881 acggctggcg aactcgcggg gcgagagcac attgaaccgc gccaatacct cgctgttggc
     5941 gagagtctgg gcggcgggta tggcggcggc tgcgtacggc gcatagggca ggccgcgcgc
     6001 ggcggcttct tcgtgccacg cgggggaata gttgtcgcca ttaaaaatga cgcgcccgtg
     6061 gtccttcatg atcttgcgga tcacgccttg cagcgccgcg ccgatttctt ccggcgtttt
     6121 tgcgccggca gcttccagct tggtggcgat gtagtcgagc gaatcggcca tgatggtgtt
     6181 gagcacgatc aacggccccg ccaccgactg gctggagccc acggtgcgga actcaaactt
     6241 gttgcccgtg aacgcgaagg ggctggtacg gttgcggtcg cccgcgtcct tgggtagtgg
     6301 cggcagcgta tccacgccga tggtcagcac gccgctcttg cgcgcccggc cactgccgcc
     6361 gcgcgcgatg ccctcgaaca catccgtcag ttgatcgcca aggaagatcg agagggtagg
     6421 cggcggcgct tcgttggcgc cgaggcggag atcgttgccc gcgtgcgcca cggaggcgcg
     6481 caggagcgtg gagtgcttat ccacggcacg gatcatcgcc gcgcaaaacg ccaggaagcg
     6541 catattgcgt tccggcgtgt cgcccggttc cagcaggttg ctgtgacggg tgcccagtga
     6601 ccaattcaaa tgtttgcccg agccgctgac gcgcgcgaac ggcttttcgt gcagcaggca
     6661 ggacatgccg tacttttgcg ccacgcgttg cagcacggtc atcgtcaggt gctgatgatc
     6721 cgccgccacg ttcgcttgct caaacacggg ggccagctcg tattgaccgg gcgccacctc
     6781 attgtggcgg gttttgacgg gtacgccgag tttgtagagc tcgcgctcga cttccagcat
     6841 gaacgccagc acgcgctcgg gaatggcgcc gaagtattga tcttccatct cctgaccttt
     6901 gggcggttgc gcgccgaaca gcgtgcgtcc ggcgctaatc aggtccggcc gcgcaaaaaa
     6961 gaagttgcgg tccaccaaaa aatattcctg ttcgcagccc gcggtggcat agaccagctc
     7021 gggattttct tcgccaaaga tgcgcagcaa ccgttgggct tgcgcgctca gcgcttgcat
     7081 ggagcgcagc agcggagttt tgttgtcaag cgcctcgccc gtccacgaac agaaggcggt
     7141 gggaatgcac agcgcggtgc cgttggggtt ttcgaggatg taggcggggc tggtcacgtc
     7201 ccaggcggtg tagccgcgcg cctcaaacgt ggcgcgaatg ccgccggacg ggaagctgga
     7261 ggcatccggc tcgccgcgga tcaattgctt ggcggaaaac gccgcaatgg cgccgccttt
     7321 gccgtccggc tccagaaaac tatcgtgctt ttcggcagtc aggcccgtca gtggataaaa
     7381 cacatgggcg tagtgggtgg cgcctttttc gatggcccaa tcgcgcatgg cggcggccac
     7441 ttcgtcggca acggtcacat ccaatttttc gccacgctcg atggtgcggc gcaacgcctc
     7501 atagaccgcc ggggagaggc gggctttcat cacgcggtcg ttgaacacgt tggagccgaa
     7561 aatttcgccc gccgatgttt tggaaaaatc cagcgcgggg gccgtggccc gataattcgt
     7621 caccgccgca atcgcattca gccttgcatt actcatgagt tgcctcgctt atcagggcat
     7681 ggaattgact attgaaaatc atatttctga tctttgaatg aataagtatt acaaatatgt
     7741 aatattggca agcatgaaaa tacaaaaatg taagaaagga attttgcgtt caattttctt
     7801 tttttggggg gaggggggaa gagcacgttg gggaccaaga ggatgcaggg gacatagggg
     7861 gtgggggata aaaaaagggc tcaggttgtc ctgaacccac ggggcttgcg aaactggtgg
     7921 aggcggcggg agtcgaaccc gcgtccgcac aagcgtcgca tc
//
LOCUS       DAOPNP010000112         7634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_10706_length_7634_cov_8.535295, whole genome shotgun sequence.
ACCESSION   DAOPNP010000112 DAOPNP010000000
VERSION     DAOPNP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10706_length_7634_cov_8.535295"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..404)
                     /locus_tag="PKI64_09220"
     CDS             complement(27..404)
                     /locus_tag="PKI64_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01217.1"
                     /translation="MKNPRFFNAIFPGLAALREVIFLFFLAKTPAGQKNANRGDFPGL
                     IVGLERSQTFQPFPAGRFHSSTIPALQKQPVGLLGTVFFLCALCVSARGYLFVFSRQD
                     ASGAKERKQRGFSGFNPEKCIWA"
     gene            complement(665..1306)
                     /locus_tag="PKI64_09225"
     CDS             complement(665..1306)
                     /locus_tag="PKI64_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01218.1"
                     /translation="MPPQKKGKPAGEHQRKRRQRCKRRKPEKNKTEQERRKNRKTGIA
                     PKKGLVLCYQANQPENINASDARDAKDASREKNKTDQERKKNRKTGKKLFGVMTRRLS
                     AFSCVPGFLIFLLILGSVAAFALKRLQSFPRCGNYVSTLWKNRVRKAKFFPHNGTTLR
                     PLIHTMEQLWGYFSTLWKIRRQKFHGVEIYLSRTGGIFYAIENLSAIFPRNGK"
     gene            complement(1308..1381)
                     /locus_tag="PKI64_09230"
     tRNA            complement(1308..1381)
                     /locus_tag="PKI64_09230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1347..1349),aa:Gly,seq:tcc)
     gene            complement(1442..2377)
                     /locus_tag="PKI64_09235"
     CDS             complement(1442..2377)
                     /locus_tag="PKI64_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01219.1"
                     /translation="MTRLLSIVLLLTLLLPGAAQAQTDTLCFVQFIHPGLEHQPDTPS
                     GRSWNTAPHRRKFLLNPGRYQTALAAAPQSGHLVFWGEYEPPTHLVQTYPVPVSDGPQ
                     YLFTPAPVPFYPNDPPLMNTDPFVFGNHFIYSICKQNNKRGPTAMQRLARGSVILFGS
                     GRGRNRFVVDTVFVVRDYVDCTLNTYREQLADVVPPEYFHAALEPIAYETQVRNLTPS
                     ATFRLYIGATVEKPFAGMYSFFPCQPAATGQPQGFARPAIHRPGIITDNLTQGQRMNP
                     QSDLAAVQELWQDVAQQILAQGLALGVHADLPRLP"
     gene            complement(2385..3326)
                     /locus_tag="PKI64_09240"
     CDS             complement(2385..3326)
                     /locus_tag="PKI64_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE01220.1"
                     /translation="MVFFFQILCVLGLVSAGFFARRRGLLSAHGTNDLAHVALSIVYP
                     ALIFVSILELSFADLRANLTLPLLVMAIAMVGFGLGLLAVRFLGRVPPETSRAFLFHC
                     LINNYSFLPLPLVLFRYGDSGVALLVFSSVGYELLLWSLGVMLFSRAERKRDRLKSLF
                     SPPFVTLLLSLGLVAARDLLPWPAPPPWLALAGETFRGGLKTLGAATIALSMLVAGSR
                     FATLKFATILGWRIWFVSAIRLVAVPLVMIPILRLMPLDPTARGILYIISVMPSAMVS
                     VLFSERYGGDTEFIAGGLLLTHLWALITVPVFLAWAF"
     gene            complement(3374..3919)
                     /locus_tag="PKI64_09245"
     CDS             complement(3374..3919)
                     /locus_tag="PKI64_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOE01221.1"
                     /translation="MLIIGLLLLLSLLLLNPASAAAPRFADGPPLVSLGVGVAQVFDN
                     HQEFFWGVEYRPAFRLFHVGPWLLFGTGKNDEFYAAAGVLADVELGRGWVVTPSFGAG
                     YYNASSGLDLGFDAEFRTAVELARRFGRGQRLGLCFAHLSNGSLSDTNPGTETLGISY
                     SFPIDFLFRRAPAAGVPDRTE"
     gene            complement(3925..4602)
                     /locus_tag="PKI64_09250"
     CDS             complement(3925..4602)
                     /locus_tag="PKI64_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01222.1"
                     /translation="MSTAQDILAGAQRWELLRKRRAKETLRSVFRVDDRYVAKQFEIP
                     LAARRYRRPWLAEDACLRRLDGRGAPRSFGWCEETVGDRRVVWLVKEYVVGAPVETFA
                     AADLPAAARLLAGVHAQAVVTDDASAGNFLRTPDGQMEFLDFGRARPYRRAGWRFDAA
                     IGCELAKLRREGFRWDAALWRAFRPLYFEALGASRGRQIRIRLACAAAVALRQARKTL
                     QGKNPRS"
     gene            complement(4613..>7634)
                     /locus_tag="PKI64_09255"
     CDS             complement(4613..>7634)
                     /locus_tag="PKI64_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE01223.1"
                     /translation="RKLSIENMPAVDIPYIAVVTQWVGASPEDVEKDVSKHVEDAVSG
                     LDGLKHIESSSLENVSQVVLEFDLAINVDTAAQDVREKIDTILTKLPADADRPVIQKI
                     NINAAPIANIFLSGDAPIDDLYDYADNVVADRFATIPGVGEVQVIGGNEREVWVELDR
                     ERLAAAGLTTADVVAALQGGILNLPGGRIREQGSEFAVRFDAEYQNVADVANLEVANR
                     DGARRKLGDLGTVRMTTAEVRQRAILDGRRGVVLKIVKKAEGNIVSLVEETRRRFAEI
                     QETLPGGMELTWISDEAENIQASVDSAVASVWQAVLLCAFILFVFLVNVRTTVVVAIT
                     MPATIVVSLFFMQLSGQTLNTPTLLAIGLSTGVLVSNSIVVLENIVSKFETMDDHWEA
                     ARLGTSEMSVAVLASAGTNVIVMLPIAMMTSLAGRMLTPFAITTLIVNAASIFISFTL
                     TPILCALLLEPASRRKDNVFARFGKRWENGFQAAAGRYVAGLRRVAGRKRWNLPIVAG
                     FVLIFLATLKFGGGRLGFTFFETADNGRIFVRVETPPYYDLEKTVARLEGIQARLLGY
                     SDLTHVLTTAGKAESMSGQASEGVYMGQIELFFKPKTARAWKIADRLTEIRNLLKDET
                     DCLISASVPGMMGGQSFQIDRNLSGEDLDVLDQTALAIQNAARDLPGVEFLETTVRDT
                     KPEIRVLPKRPILSDLGLTPALLGTIVRANVEGIEAADFKRGDRTYDVRVKLAEQSGK
                     DQIRQFLLPGADGRPVPLETVADVIEARVKTQIYRYDKRRTVKILGDIQPGATMSGVG
                     AEVARTITEQKLLPAGYAMDNAGFSEMMDETVADFGEAILLAAFLTLLTLAAILESWS
                     RPALVLLTLPMGLVGVIWALVLGHHAITMLVLLGVLMLIGVVVNAAILIVDKMAQHIR
                     EGVGRREAMLTAIAEEFRPVLMVVLASGLGMLPMAIGTGIGSESRAGIGLASVAGVLV
                     AGVLTMTVLPLIYTLFTGRPKGESLAKSGPPCED"
BASE COUNT         1433 a         2101 c         2759 g         1341 t
ORIGIN      
        1 gtgttcatcg ggtgatgacg caacccctat gcccaaatgc atttttcggg attaaacccg
       61 gaaaatcccc tctgtttgcg ttcttttgcg ccgctggcgt cttggcgaga aaaaacaaaa
      121 agataacctc gcgcagagac gcaaagcgcg caaaggaaga aaacagtccc caacaatcca
      181 accggctgct tttgtagggc aggaatggtt gaagaatgaa accttcccgc cgggaacggc
      241 tggaaggtct gcgaccgttc cagtcctaca attaaacccg gaaaatcccc tctgtttgcg
      301 ttcttttgtc ccgctggcgt cttggcgaga aaaaacaaaa agatgacctc gcgcagagcc
      361 gccaagcccg gaaaaatcgc attaaaaaag cgcggatttt tcatactacc ttgtcatcca
      421 aggtaccttg tgtcacaagg tagtggtctc ccctgggtgg ggtgggggaa aatggcgctg
      481 attgcgcgac cagcaacgcc ccatagccgg cttttccaca ctgtggaaaa actcgcccgc
      541 gcgggataaa ctgacctcaa tggttgccgc tgcgctcgct atggctcgct ggctcgcccg
      601 gctgtgcggt cgctatagag ctcgcgttcg cccggcgtcc ttcggacttg ggctcggcac
      661 tcgcctattt tccattgcgt ggaaaaatcg cggaaaggtt ttctattgcg tagaaaatcc
      721 cgccggtgcg ggataaataa atttccacac cgtggaactt ttgccgccga attttccaca
      781 gtgtggaaaa gtacccccaa agttgttcca ttgtgtggat aagtggcctc aaagttgttc
      841 cattgtgtgg aaaaaatttg gccttgcgca ccctgttttt ccacagtgtg gaaacataat
      901 ttccacaccg tggaaaactt tgaagccgtt tcaacgcaaa cgccgcaacc gacccaagaa
      961 tcagcaggaa aataagaaac ccaggaacgc aggaaaaggc agaaaggcgc cttgtcatca
     1021 caccgaaaag tttctttcct gttttcctgt ttttcttgcg ttcctgatcg gttttatttt
     1081 tttcccggct tgcgtctttt gcatctctgg cgtcgcttgc gttaatgttc tctggctggt
     1141 ttgcctgata acataggact aagccctttt ttggggctat tcctgttttc ctgtttttcc
     1201 tgcgttcctg ctcggtttta tttttttccg gcttgcgtct tttgcatctc tggcgtcgct
     1261 tgcgttgatg ttctccggct ggtttgcctt ttttttgggg gggcaagtgg agcgggcgat
     1321 gggaatcgaa cccacgtagc cagcttggaa ggctggagct ctaccattga gctacgcccg
     1381 cgaaggatgc aagttggcgc gcattgtgcg cgaggggtcg ggatgggtca agtcccggat
     1441 ttcagggcag gcgcggcagg tcggcgtgta cgccgagcgc caagccctgc gcgaggattt
     1501 gctgcgccac gtcctgccac agctcctgca cggcggcgag gtcggattgg gggttcatcc
     1561 gttggccttg ggtgaggtta tcggtaataa ttccggggcg gtggatggcg gggcgggcga
     1621 agccttgcgg ctggccagtg gcggccggct ggcaggggaa aaagctatac atcccggcaa
     1681 agggtttttc caccgtggcg ccgatataca gccggaaggt ggcggagggg gtgaggttgc
     1741 ggacttgcgt ttcgtaggcg atgggttcca gcgcggcgtg gaaatactcg ggtggcacta
     1801 cgtcggccaa ctgttcgcgg taggtgttga gggtgcagtc cacgtagtcg cgcaccacaa
     1861 aaacggtgtc caccacgaag cggttgcggc cgcggccgga gccgaagagg atgacggaac
     1921 cgcgggccag gcgctgcatg gcggtggggc cgcgcttgtt gttttgcttg cagatgctgt
     1981 agatgaaatg gttgccaaag acaaacgggt cggtgttcat caggggcgga tcgttgggat
     2041 aaaaaggaac gggggcgggg gtgaagagat attgcgggcc atcggaaacg gggaccggat
     2101 aggtttgcac aaggtgggtg gggggctcgt attcacccca aaaaacgagg tggccggatt
     2161 gcggggccgc agcgagggcg gtttggtagc gcccggggtt cagcaaaaac tttcggcggt
     2221 gcggggcggt gttccacgag cggccggagg gggtatcggg ctggtgctca aggccgggat
     2281 ggatgaattg cacaaaacac agggtgtcgg tctgggcttg ggccgcgccg ggcagcagga
     2341 gggtcagcag cagcacgatg gaaagcaagc gcgtcatggc cacctcaaaa agcccacgcc
     2401 aggaagaccg gcacggtgat caacgcccac aggtgggtca gcagcaggcc gccggcaatg
     2461 aattcggtat cgccgccgta gcgctcggaa aacagcacgc tgaccatggc ggagggcatt
     2521 acggaaatga tatacaggat gccgcgggcg gtggggtcga gcggcatcag ccgcagaatg
     2581 gggatcatta cgagcggcac ggccacgagc cggatggcgg aaacgaacca gatgcgccaa
     2641 ccgaggatgg tcgcgaattt cagcgtggcg aaccggctgc cggcaaccag catggacagg
     2701 gcgatcgtgg cggcgccgag ggtcttgagg ccgccgcgga aggtttcgcc cgccaaggcc
     2761 agccacggcg gcggcgcggg ccagggcagg aggtcgcggg cggcgacgag gcccaagctg
     2821 agcagcagcg tgacgaacgg cgggctgaac agggacttga gccggtcgcg cttgcgctcg
     2881 gcgcgggaga acagcatgac gccgagcgac cacagcagga gctcgtagcc gacggaggaa
     2941 aacaccagca gggccacgcc ggagtcgccg tagcggaaca gcacgagcgg caggggcagg
     3001 aacgagtagt tgttgatcag gcagtggaaa agaaaggccc gggacgtttc cggcggcacg
     3061 cgcccgagga agcgcacggc cagcaggccc agcccgaagc cgaccatcgc gatggccatg
     3121 acgagcaggg gcagcgtcag gttcgcgcgc agatcggcga aggacagttc gaggatcgag
     3181 acgaagatga gcgcgggata gacgatggag agcgcgacgt gggcaaggtc gttggtgccg
     3241 tgcgcggaga gcaggccgcg ccgccgggcg aagaagccgg cggaaacgag cccgagcacg
     3301 cacaggatct ggaagaagaa caccatgaac gcccactata ccccaagcgg gccggcgggg
     3361 caaagccgac cgttcattcc gtccggtccg gaacgccggc ggcgggcgcg cggcggaaca
     3421 gaaaatcgat cggaaaagaa tacgagatgc ccagggtttc ggtgccggga ttggtgtcgc
     3481 tcagggagcc gttggagaga tgggcgaagc agagccccag gcgctggccg cggccgaagc
     3541 gccgggccag ttcgacggcg gtgcggaatt cggcgtcgaa gccgaggtcg agcccgctgg
     3601 aagcgttgta gtagccggcg ccgaaactgg gggtgaccac ccagccgcgg ccgagttcga
     3661 cgtccgccag cacgccggcg gcggcataga attcgtcgtt tttgccggtg ccgaacagca
     3721 accaggggcc gacgtggaaa agccggaagg ccggtcggta ttcgacgccc cagaaaaatt
     3781 cctggtggtt gtcgaacacc tgggccacgc cgacgccgag ggagaccaga ggcgggccgt
     3841 cggcgaaccg gggggcggcg gccgaagcgg gattcagcag gagaagcgac agcagaagca
     3901 gtaggccgat gatcagcatg gttctcagct ccgcgggttt ttcccttgca gggtcttgcg
     3961 cgcttggcgc agcgcgacgg cggccgcgca ggcgaggcgg atgcggattt gccggccgcg
     4021 cgaagcgccc agcgcctcga aatagagcgg ccggaaggcg cgccacagcg cggcgtccca
     4081 gcggaagcct tcgcgccgca gcttggcgag ttcgcagccg atggcggcat cgaagcgcca
     4141 tcccgcgcgc cggtaggggc gggcgcggcc gaaatcgagg aattccatct ggccgtcggg
     4201 ggtgcggagg aaatttccgg cgctggcgtc gtcggtgacg acggcctggg cgtggacgcc
     4261 cgccagcagg cgggcggcgg cgggcagatc ggccgcggcg aaggtttcga cgggcgcgcc
     4321 aacgacgtat tccttcacca gccaaacgac gcgccggtcg ccgacggttt cctcgcacca
     4381 gccaaaggaa cgcggcgcgc cgcgcccgtc gagccggcgc aagcaggcgt cttcggcgag
     4441 ccaggggcgg cgatagcggc gcgcggccag cgggatctcg aattgcttgg cgacgtagcg
     4501 gtcgtccacg cggaagacgg agcgcagggt ttccttggcg cggcgtttgc gcaggagttc
     4561 ccagcgctgg gcgccggcaa ggatgtcttg ggcggtggac atggaacgac cgtcaatctt
     4621 cgcaaggcgg gccggatttc gcaagcgact cgcccttggg ccggccggtg aagagggtgt
     4681 agatcagcgg caggacggtc atggtgagga cgccggccac gaggacgccg gcgacggaag
     4741 cgaggccgat gccggcgcgg ctttcggagc cgatgccggt gccgatggcc atgggcagca
     4801 tgccgaggcc ggaggcgagc accaccatca gcacggggcg gaattcctcg gcgatggcgg
     4861 tgagcatggc ctcgcggcgg ccaacgcctt cgcggatgtg ttgggccatt ttgtccacga
     4921 tcaggatggc ggcgttcacc accacgccga tgagcatgag gacgccgagc agcacgagca
     4981 tggtgatggc gtggtggccg agcacgagcg cccagatgac gccgacgagg cccatgggca
     5041 gggtcaggag gacgagggca gggcgcgacc acgactccag gatggcggcg agcgtgagca
     5101 gcgtgaggaa cgcggccaac aggatggctt cgccgaaatc ggcgacggtt tcgtccatca
     5161 tctcgctgaa gccggcgttg tccatggcgt agcccgccgg caggagtttt tgctcggtga
     5221 tggtgcgggc cacctcggcg ccgacgccgc tcatggtggc gccgggctgg atgtcgccga
     5281 ggatcttcac ggtgcgccgc ttgtcgtagc gatagatctg ggtcttgacg cgggcctcga
     5341 tgacgtcggc gacggtttcg agggggacgg ggcggccatc ggcgccgggc agcaggaatt
     5401 ggcggatctg gtccttgccg ctttgttcgg ccagcttgac gcggacgtcg taggtgcggt
     5461 cgccgcgctt gaagtcggcg gcctcgatgc cttcgacgtt cgcgcgcacg atggtgccga
     5521 gcagggcggg ggtgaggccg aggtcggaca ggatcggccg cttgggcaac acgcggattt
     5581 cgggcttggt gtcgcggacg gtggtttcga ggaattcgac gcccggcagg tcgcgcgcgg
     5641 cgttctggat ggccaaggcg gtttggtcga gcacgtcgag gtcctcgccg gagagattgc
     5701 ggtcgatctg gaaactttgg ccgcccatca tgccggggac ggaggcggag atcaggcagt
     5761 cggtttcgtc cttcaacaga ttgcggattt ccgtcaggcg gtcggcgatt ttccaggcgc
     5821 gcgcggtctt gggcttgaag aacaactcga tctggcccat gtagacgcct tcgctggctt
     5881 ggccgctcat ggattcggcc ttgccggcgg tggtcaggac gtgcgtcagg tcggaatagc
     5941 ccagcaggcg cgcctggatg ccttcgaggc gcgcgacggt tttctcgaga tcgtagtagg
     6001 gcggagtttc cacgcggacg aagatgcggc cgttgtcggc ggtttcgaag aaggtgaagc
     6061 cgaggcggcc gccgccgaac ttcaaggtgg ccaggaatat caggacgaag ccggcgacga
     6121 tgggcaggtt ccagcgtttc cggccggcca cgcggcgcag gcccgcaacg tagcggccgg
     6181 cggcggcctg aaagccgttt tcccagcgct tgccgaagcg ggcgaagacg ttgtccttgc
     6241 gccgggaggc gggttcgagc agcagggcgc agaggatggg ggtgagggtg aagctgatga
     6301 agatggacgc ggcgttcacg atcagggtcg tgatcgcgaa gggggtgagc atgcgtccgg
     6361 ccaggctggt catcatggcg atgggcagca tcacgatgac gttggtgccg gcggaggcga
     6421 gcacggccac ggacatttcg gaggtgccga ggcgcgccgc ctcccagtgg tcgtccatgg
     6481 tctcgaactt cgaaacgatg ttttcgagca cgacgatgga attggacacc agcacgccgg
     6541 tggacaggcc gatggcgagc agcgtgggag tattgagggt ttggccggac aactgcatga
     6601 agaacaggct gacgacgatg gtggccggca tggtgatcgc cacgacgacg gtggtgcgga
     6661 cgttgaccag gaagacgaac aggatgaacg cgcaaagcag tacggcctgc cagacgcttg
     6721 cgacggcgga atcgacggag gcctggatgt tttcggcttc gtccgaaatc caggtcagtt
     6781 ccatgccgcc gggcagggtt tcctggattt ccgcgaagcg ccgccgggtt tcctccacca
     6841 gcgaaacgat gttgccttcg gccttcttga cgatcttgag cacgacgccg cgccggccgt
     6901 cgaggatggc gcgctggcgc acttccgcgg tggtcatgcg gacggtgccg aggtcgccca
     6961 gcttgcggcg ggcgccgtcg cggttggcca cctcgaggtt ggcgacgtcg gcgacgttct
     7021 ggtactcggc gtcgaagcgg acggcgaatt cgctgccttg ctcgcggatg cggccgccgg
     7081 gcaggttgag aatgccgccc tggagcgcgg cgacgacgtc ggccgtggtg aggccggcgg
     7141 cggccaggcg ttcgcgatcg agttccaccc acacttcgcg ttcgttgccg ccgatgacct
     7201 gcacttcgcc gacgccggga atcgtggcga agcggtcggc gacgacgttg tcggcgtagt
     7261 cgtagaggtc gtcgatcggc gcgtcgccgg agaggaaaat attggcgatg ggcgcggcgt
     7321 tgatgttgat tttctgaatg acggggcgat cggcatcggc cggcagtttg gtgaggatcg
     7381 tgtcaatttt ttcgcggaca tcctgtgcgg cagtgtccac attgatggcc agatcaaact
     7441 ccaggaccac ctggctgacg ttctccaagc tggaggactc aatgtgcttg aggccatcga
     7501 ggccggagac ggcgtcttcg acgtgcttgc tgacgtcttt ttcgacgtct tccggagaag
     7561 cgccgaccca ttgggtgacg acggcgatgt aggggatgtc gacggccggc atgttctcga
     7621 tggagagctt gcgg
//
LOCUS       DAOPNP010000113         7424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_11070_length_7424_cov_10.518116, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000113 DAOPNP010000000
VERSION     DAOPNP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11070_length_7424_cov_10.518116"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1270
                     /locus_tag="PKI64_09260"
     CDS             164..1270
                     /locus_tag="PKI64_09260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE01224.1"
                     /translation="MSRPLKVALAHDWLNGMRGGERCLDLIGREYPAAELYTLLYQPE
                     QVSEAIRRRTVHVSGLQRVPGFRRHYRWFLPLFPMAMESFRLPAGTDLVISTSHCVAK
                     GIRPPPGAKHLCYCFTPMRYAWALQEDYFGRGGLKRALIDRLLARLRRWDVAANERVD
                     RFVAISRHVQKRIETFYGREAAVVYPPVATQFFTPGDAAGSGDYDLIVSALVPYKRVD
                     LAVRAYAQLGTPLKIAGVGTEWDALRASAPPNVEFLGRVPDEGIRELYRGCRFLIFPG
                     EEDFGIVPLEAQACGKPVVALGRGGLLETVVAGTTGVFFAEQTPEALAAAVRDAAQRE
                     WQPATIRAHAEQFSEARFLDGLRAEINKLLAGRG"
     gene            1267..1932
                     /locus_tag="PKI64_09265"
     CDS             1267..1932
                     /locus_tag="PKI64_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01225.1"
                     /translation="MNAAGANSFSAGQQLRLARGFSSAFWSLPLMAALHAAALARLGP
                     AAWVVTGLPGSFLPLGWGLWLMMSVAPDDARWRSRVRCVGWLTLITAWLSPFLPWWLG
                     VPQLDYLAVNAGLHYLLLIACLMSLNGLAAAYAARTGDTALRREAFAGLWMVLWLSLC
                     TVGVLLWLFHRAGLLGAGLPAILRELAELPREAQSLFLLPYAMTAYVLWRAKETGFRL
                     AAE"
     gene            complement(2050..4344)
                     /locus_tag="PKI64_09270"
     CDS             complement(2050..4344)
                     /locus_tag="PKI64_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01226.1"
                     /translation="MNLFTTQRDLPPLLTGLLRWLRRLGIAAVALVVVYLVLEVICTY
                     ALPQCGSRFLVPGEVNGEPVWRDNPFFAYRFYPPRTAPAPLPIVARQTPAPGTLRVCL
                     LGGDALMGEPEPSFGPGRQLELMLRQRYPDHPVEVIHLTLEGGNSHIMREVARDLERL
                     RPQAVVIMIGNEEIAGPYGPASTLGWFHHSARLARTLVLFSRTHVSQLFAGLVGRVAP
                     ARVDLNVWRSQEPLMLKGRLAPDDPRLKSAYRAFNRNLRAILRLAKAAAPEVIVCTVP
                     VNLRNCDPFLTTYLPDEAAAQQVRETLRNAIAADAAGAPEAARLYADVIRRNPTHAEA
                     LFRAARLALADGRRAEAFDFFVRARDADSLRLRTDSRQNQMIRAAAEEAGVSLLDLEK
                     HFALSSPKGVPGHEFFLDHVHFTFAGANLLAAAVLARLESRQAFDAIPTGALPDAPTL
                     ATELLYDPWGHAAVLKTVLDQQLRPPFNRQVDNAAYRQELMNDRQAAEARVTSLSAAN
                     TKAILKRRLALAPADGWLAARAGWYLFNAGATNEAIAAAETAHQLWPHRYEPRALLAF
                     LQTVAGAEVEESIAFLKGADYAGYYDVNWALRIGEELTARGLPLAARPWLEYALTRDP
                     WNSAVAIALSQALQKAKLGQEAVDVLQAAIKRNPQNPLLWEELAALYCVLGRWKLSTQ
                     CFNKVEEIAPFRYERFYKWADALVRLKQYSRALGPINRYLAAMPDDPEGRALLAIIKD
                     HIPAGSEPPQDNDDEKPTRKLPWE"
     gene            4604..7351
                     /gene="ppdK"
                     /locus_tag="PKI64_09275"
     CDS             4604..7351
                     /gene="ppdK"
                     /locus_tag="PKI64_09275"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOE01227.1"
                     /translation="MAKKKYVYFYGFGKENTEGDASLKAILGGKGANLAEMANANLPV
                     PAGFTVSTEACAYYSSHKGKYPEGMLDQLNAQLKKLEKKMGKKLGDPKNPLLVSVRSG
                     AAISMPGMMDTVLNLGLNDKSILGFIASTGNDRAAWDSYRRFIDMFGDVVMGPTTGLE
                     HHDFEVAMADLKKKYGAKEDTDLTADQLKELCDIYKKVYQQKVKKPFPQDPMDQLLAA
                     VNAVFGSWNSERAQKYRQINKVTGLLGTAVNVQAMVFGNMGDESGTGVAFTRDGSTGS
                     EEPMGEYLVNAQGEDVVAGIRTPRHLDEMPSEKSPIWKKAHEQLIKIMKRLEEHYKYP
                     QDVEFTVEQGTLWMLQTRNAKRSGLAGVRWAVEMATGKDFYTGKPQKKILRPDEAIAT
                     VTGSDLEQLLYPIFDAAAEKKAVKLTTGLPAGPGAGVGKIVFEAHVAEEMVKANPDEK
                     LILVRHETSPEDVGGMWAAQGILTSTGGMTSHAAVVARGWGKCCIAGASALQIDYKAK
                     TISAGGTTLKEGDWISLNGTAGVVYVGQIPTQASPVVNAVVGGKKDAQKHPIYRMYKQ
                     VSDWADKYRKIAVRTNADSPKDAAAARAFGAQGIGLCRTEHMFFEGDRIWAVREFILA
                     QDTAAREKALAKLLPLQRSDFEGIFKAMDGYGVTIRLIDPPLHEFVPQDEAGQAEMAK
                     RMGTTPDVVAAHVKKLHEMNPMLGHRGCRLSITYPELCVMQTRAIIEAACNVAKTGRK
                     VLPEIMVPLVGAKAELDYCAEIIRQTADAIIKEKKAKVKYLVGTMVEVPRAALTADAI
                     AETAEFFSFGTNDMTQMTFGFSRDDIGTFLPDYLDKKILPVDPFQSLDTTGVGQLVEM
                     AVKKGRSTRPDLKCGICGEHGGDPASVKFFAKVGLNYVSCSPYRVPIARLAAAQQALG
                     K"
BASE COUNT         1303 a         2296 c         2552 g         1273 t
ORIGIN      
        1 cgccagacga tttggcgggc gcggtggcgg cgttgcgcca cgtgctgacg gactccgcgg
       61 aggccgcccg cctgcgcgcg gcgggattgg cccgggcgcg ggcgttcact tggcgcacaa
      121 cgacggccgc ggtgctgcaa acctacgcgg aggtgttggc gtcatgagtc gcccgctcaa
      181 agtggcgctg gcgcacgact ggctcaacgg gatgcgcggg ggcgaacggt gcctcgacct
      241 gattggccgc gaatatccgg cggcggagct atacacgctg ttgtatcagc cggagcaagt
      301 gtcggaggcc attcgccggc gtacggtgca tgtgtccggc ttgcagcggg tgccgggatt
      361 ccgccgccac taccgttggt ttttgccgct cttcccgatg gcgatggaat cgttccgcct
      421 gcctgcgggc acggatttgg tgatcagcac gagccattgc gtggcgaagg ggatccgccc
      481 gccgcccggc gccaagcacc tgtgctactg cttcacgccg atgcgctacg cctgggcact
      541 gcaagaggat tacttcgggc gcggcgggct caagcgcgcg ctgattgacc gcctgttggc
      601 ccggctgcgg cgctgggatg ttgcggccaa cgagcgtgtg gaccggtttg tggcgattag
      661 ccggcacgtg caaaagcgca ttgagacttt ttacggtcgc gaagcggcgg tggtttatcc
      721 gccggtggcc acgcagtttt tcacgcccgg cgatgccgcg gggtcgggag actacgattt
      781 gattgtgtcg gcgctggtgc catacaagcg cgtggatttg gcggtgcggg cctatgcgca
      841 gttgggcacg ccactgaaga ttgccggcgt gggcacggag tgggacgcgc tgcgcgcgag
      901 cgcgccaccg aatgtggaat ttctcggccg cgtgccggat gagggtattc gcgagttgta
      961 ccgcggctgc cggtttttga tttttcccgg cgaggaagat ttcggaattg tgccgctgga
     1021 agcgcaggcg tgcggcaagc cggtggtggc attgggccgt ggtggcttgc tggaaaccgt
     1081 ggtggcggga accacgggcg tattttttgc ggagcagacg ccggaggcgt tggccgcggc
     1141 ggtgcgcgat gccgcgcaac gggaatggca gccggcgacc atccgtgcgc acgcggagca
     1201 attcagcgag gcgcgttttc tggacgggct gcgcgcggaa atcaacaagt tgctggcagg
     1261 gcgcggatga acgcggcggg cgcgaacagt ttttcggctg ggcagcaact ccggctggcg
     1321 cgcggttttt ccagcgcgtt ttggagcctg ccgctgatgg ccgcgttgca cgcggcggcc
     1381 ttggcgcggc tgggccccgc ggcgtgggtg gtgaccggtt tgccgggcag ctttttgcct
     1441 ctggggtggg gcctgtggct gatgatgagc gtggcgccgg acgatgcgcg gtggcggtcg
     1501 cgagtgcgtt gcgtcggttg gctgacgttg atcacggcgt ggctctcgcc atttctgccg
     1561 tggtggctgg gcgtgccgca gttggactat ctggcggtca acgcggggct gcactatctg
     1621 ctgttgattg cctgcctgat gagcctgaac ggtctcgcgg cggcctatgc cgcgcgcacg
     1681 ggcgacaccg cgttgcgccg cgaggcgttt gccgggcttt ggatggtgct gtggttgagc
     1741 ctgtgcaccg tgggcgtttt gttgtggctg tttcaccgcg cggggttgct gggcgcgggg
     1801 ctgccggcca ttttgcgcga gctggcggaa ttgccgcgtg aggcgcaatc gctgtttctg
     1861 ctgccgtacg ccatgacggc ctatgtgctc tggcgcgcga aggaaactgg cttccggctg
     1921 gccgcggaat aatcagccgc attcacgcgg gcttttgtcg cgcgtgaatg gttaaggcac
     1981 gaaactttcc tgtcgggaac ggctgaaaga tctgggaccg ttccagccct atataaccga
     2041 ccggctgcgt tattcccacg gcagtttgcg cgtgggtttt tcgtcgtcgt tgtcttgggg
     2101 tggctcggag ccggcgggga tatgatcctt aataatggcc agcagggcgc ggccttcagg
     2161 atcatccggc atggcggcga ggtagcggtt gatgggtccc agcgcgcgcg aatattgctt
     2221 gaggcggacg agagcatcgg cccatttgta gaagcgctcg tagcggaagg gagcaatttc
     2281 ctccaccttg ttgaagcact gggtggagag tttccagcgg cccagcacgc agtagagcgc
     2341 ggccagctct tcccacagca gcgggttttg cggattgcgc ttgatggccg cttgcaggac
     2401 atccaccgcc tcctggccca gcttggcttt ttgcagcgct tgggagagag cgatggccac
     2461 cgcggagttc catgggtcgc gcgtcaacgc atattccagc caaggccgtg cggccagcgg
     2521 caggccgcgc gcggtgagct cctcaccgat gcgcagcgcc cagttcacat cgtaatagcc
     2581 ggcgtagtcg gcgcccttga gaaacgcgat gctttcttcg acctcggcgc cggcgacggt
     2641 ttgcaggaac gccagcagcg cgcgcggttc gtagcggtgc ggccagagtt ggtgggcggt
     2701 ttcggcggca gcgatggcct cgttggtggc gccggcgttg aacagatacc agcccgcgcg
     2761 cgcggccagc cagccatcgg cgggggcgag ggcgaggcgg cgcttgagaa tggccttggt
     2821 gttggcggcg gagagactgg tcacgcgcgc ttcggcggct tggcggtcat tcatcaattc
     2881 ctggcgatag gccgcgttat ccacttgccg gttgaacggc gggcgtagtt gctgatccag
     2941 cacggtcttg agcacggcgg cgtggcccca cggatcatag agcagctcag tggcgagggt
     3001 gggggcatcg ggcagcgcgc ccgtggggat ggcatcgaac gcctgccgcg attccagccg
     3061 cgcgagcacg gcggccgcca gcaggttggc gcccgcgaac gtgaagtgca cgtggtccaa
     3121 aaagaattcg tgccccggca cgcccttggg cgagctgagc gcaaaatgtt tttccaaatc
     3181 caacagggaa acgccggctt cttcggcggc ggcgcggatc atctggtttt ggcgtgaatc
     3241 ggtgcgcagg cgcaggctgt cggcatcgcg cgcgcggaca aagaaatcga acgcctcggc
     3301 gcggcgaccg tcggccagcg cgaggcgcgc ggcgcggaag agcgcctcgg cgtgggtggg
     3361 attgcggcgg atgacgtcgg catagaggcg ggcggcttcg ggcgcgccgg cggcatcggc
     3421 ggcgatggcg ttgcgcaggg tttcgcgcac ctgctgggca gcggcctcgt cgggcagata
     3481 ggtggtgagg aacggatcgc aattgcgcag gttgaccggc acggtgcaga caatcacttc
     3541 cggcgcggcg gccttggcca gccggaggat ggcgcgcagg ttgcggttga acgcgcgata
     3601 ggcgcttttc agccgcgggt catccggcgc aagccggccc ttgagcatca gcggctcctg
     3661 gctgcgccac acgttgaggt ccacgcgcgc cggcgccacg cgccccacca gccccgcgaa
     3721 gagttgcgaa acgtgcgtgc gcgagaacag caccagcgtg cgcgccagcc gtgcgctatg
     3781 gtggaaccag cccagcgtgg aggccgggcc gtagggaccg gcgatttcct cgttgccgat
     3841 catgatcacc accgcctgcg ggcgcagtcg ttcgagatca cgcgccactt cgcgcatgat
     3901 gtgcgagttg ccgccttcga gcgtgaggtg gatcacctcc acggggtggt cggggtagcg
     3961 ctggcgcagc atgagctcga gttgccggcc ggggccgaag gaaggttcgg gctcgcccat
     4021 cagcgcatcg ccgcccagca ggcaaacgcg cagcgtgccc ggcgcggggg tttggcgcgc
     4081 cacaatcggc aacggggcgg gggcggtgcg tggcggatag aagcggtagg caaaaaacgg
     4141 gttatcgcgc cagacgggtt cgccgttcac ttcgccgggc accaggaagc ggctgccgca
     4201 ttgggggagg gcgtaggtgc agatgacttc cagcaccaga tacaccacga ccagcgccac
     4261 cgccgcgatg cccaatcggc gcagccagcg caggaggcca gtcagcaggg ggggcagatc
     4321 gcgttgggtg gtgaacagat tcatgtccgg cgtgtaatat cgaatatagg agagccccgc
     4381 tttattgtaa agcggccttg caaaaccgcg cgcgcggcgg ccggcgcgcc cgcagggtaa
     4441 atcgtttcga gcttggcggt ttcgccatgt tttgccgccg gctatttact tgacaaatcg
     4501 cccaaggcga tggatagtcg cgcggatttt ttccacccgg cttgccgcgt ggtatccctt
     4561 tagaaaaaaa tcggccggcc acggccgcaa cacggagaca aacatggcga agaagaaata
     4621 tgtgtatttt tacggattcg ggaaggagaa caccgagggc gatgcctcgc tgaaggccat
     4681 cctgggcggc aagggcgcga acctcgccga aatggcgaac gccaacctgc ccgtgcccgc
     4741 gggcttcacg gtcagcaccg aagcgtgcgc ctactattcc tctcacaagg gcaagtatcc
     4801 cgaggggatg ctcgaccagc tcaacgccca gctcaagaaa ctcgaaaaga aaatgggcaa
     4861 gaagctcggc gaccccaaga acccgctgct ggtttccgtg cgttccggcg cggccatctc
     4921 catgcccggc atgatggaca ccgtcctcaa cctcggcctc aacgacaagt ccatcctcgg
     4981 cttcatcgcc agcaccggca acgatcgcgc cgcgtgggac tcctaccgcc gcttcattga
     5041 catgttcggc gatgtggtca tgggccccac caccggcctc gaacaccacg acttcgaagt
     5101 ggccatggcc gatctgaaga aaaaatatgg cgccaaggaa gacaccgatc tgaccgccga
     5161 ccagctcaag gagctgtgcg acatttacaa gaaggtctac cagcagaaag tcaagaagcc
     5221 cttcccgcag gacccgatgg accagctcct tgccgccgtc aatgcggtgt tcggcagttg
     5281 gaacagcgag cgcgcgcaga agtaccgcca gatcaacaag gtcaccggcc tgctcggcac
     5341 cgccgtcaac gtgcaagcca tggtgttcgg caacatgggc gatgaatccg gcaccggcgt
     5401 cgcgttcacc cgcgacggct cgaccggttc cgaagagccc atgggcgaat acctcgtcaa
     5461 cgctcaaggc gaagacgtgg tggccggcat ccgcaccccg cggcacctcg acgaaatgcc
     5521 cagcgaaaag agccccatct ggaagaaggc gcacgaacag ttgatcaaga tcatgaagcg
     5581 cctcgaagag cactacaagt acccgcagga cgtggagttc accgtcgagc agggcacgct
     5641 gtggatgctg caaacccgca acgccaagcg ctccggcctc gccggtgtgc gctgggccgt
     5701 cgaaatggcc accggcaagg acttctatac cggcaagccg cagaagaaaa tcctgcgccc
     5761 cgatgaagcc attgccaccg tcaccggctc cgacctcgag cagttgctct accccatctt
     5821 cgacgctgcc gccgagaaga aggccgtcaa gctcaccacc ggtctgccgg cgggtccggg
     5881 cgcgggcgtg ggcaagatcg tgttcgaggc gcacgtcgcc gaagaaatgg tcaaggccaa
     5941 tccggatgag aagttgatcc tcgtgcgcca cgaaaccagc cccgaagacg tcggcggcat
     6001 gtgggccgcg cagggcattc tgaccagcac gggcggcatg acctcccacg cggccgtggt
     6061 ggcccgcggc tggggcaagt gctgcattgc cggcgccagc gcgctgcaaa ttgactacaa
     6121 ggccaagacg atctccgccg gcggcaccac gctgaaggaa ggcgattgga tcagcctcaa
     6181 tggcaccgcc ggcgtcgtct acgtcggcca aatccccacc caggccagcc ccgtggtcaa
     6241 cgcggtggtc ggcggcaaga aggatgccca aaagcacccc atctaccgca tgtacaagca
     6301 agtcagcgac tgggccgaca agtaccgcaa gattgccgtg cgcaccaacg ccgacagccc
     6361 caaggatgcc gccgccgccc gcgcgttcgg cgcgcagggc atcggcctgt gccgcaccga
     6421 gcacatgttc tttgaaggcg accgcatctg ggccgtccgc gaattcatcc tggcgcagga
     6481 caccgccgcg cgtgagaagg cgctggccaa gctgctgccg ctgcaacgca gcgacttcga
     6541 aggcatcttc aaggccatgg atggctacgg cgtgacgatc cgcctgattg acccgccgct
     6601 gcacgagttc gtgccgcagg atgaggccgg ccaggccgag atggccaagc gcatgggcac
     6661 cacgcccgac gtggttgccg cgcacgtcaa gaagctgcac gagatgaacc ccatgctggg
     6721 gcatcgtggc tgccgcctct ccatcaccta tccggaattg tgcgtcatgc aaacccgcgc
     6781 catcattgag gccgcctgca acgtcgccaa gaccggccgc aaggtcctgc cggaaatcat
     6841 ggtgccgctc gtgggcgcca aggccgagct cgactactgc gccgaaatca tccgccagac
     6901 cgccgatgcc atcatcaagg aaaagaaagc caaggtgaag tatctcgtcg gcaccatggt
     6961 cgaagtcccg cgcgcggcgc tcaccgccga cgccatcgcc gaaaccgccg agttcttcag
     7021 cttcggcacc aacgacatga cccagatgac cttcggcttc agccgtgacg acatcggcac
     7081 tttcctgccc gactacctcg acaagaagat tctgccggtg gacccgttcc agtcgctgga
     7141 caccacgggc gtcgggcagt tggtggagat ggccgtcaag aagggccgca gcacgcgccc
     7201 ggacctcaag tgcggcattt gcggcgaaca cggtggcgat ccggcctccg tcaagttctt
     7261 cgccaaggtc ggcttgaact acgtgagctg ctcgccatac cgcgtgccca tcgcgcgcct
     7321 cgccgcggct cagcaggccc tcggcaagta atcgcgcctc gcgccacaaa ccgcctccgg
     7381 ctaccggggg cggttttttt gttgccgcca cacgttgcct cacc
//
LOCUS       DAOPNP010000114         7320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_11270_length_7320_cov_10.271576, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000114 DAOPNP010000000
VERSION     DAOPNP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11270_length_7320_cov_10.271576"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..2052)
                     /locus_tag="PKI64_09280"
     CDS             complement(136..2052)
                     /locus_tag="PKI64_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOE01228.1"
                     /translation="MTQFADGKVIHSSARRWYDALTLLLLMWPATGGMWLLGSTRTWA
                     FAPGLVLAFCGIALAASRPLWFADTPAWRWPAGWWIFAALTTYVVALAPSAMVPYAAR
                     WEALKWLCLLGALWSWTQLANQPRRWRVLVFILLLSVALICLYAIVQRVGGSRQVLWA
                     PRPEQYGLRASGTYLCPNHLANVLATLFPLAILLLFCADAGFPLRLMGLYFLGVATPV
                     LYWTQSRSAWAGLLVGCITSLLLLAWRKSRALFALALVALPLLAAGGGWVAWRTLPLV
                     QERVQPVLERGLEAGGGRLDMWRDSLVMIRERSITGFSGGSFVWAYPPYQKHVKYHLL
                     WDYLHNEYLQLQVEYGAIGSGLLVAGLLWGGIGLLVAFLRAQSAGTRVLLAAAAGAVA
                     GNMVHALFDFNFHIFPNPHVLAWLIGVAWGAWFLEEGWRKKARPQARGWRMAVAGGAV
                     LVCLCAAWRATAAGLSYYWNLRGDIARSSLNWDAAAADYARASRWDARNWQPHAGLGM
                     LRQIQAFWYRDPDEAAEKAGREELATEAAVHLRRAAELNPGDMAVVYTLGLVHNLLDD
                     PEGALAEFRRAAAYQHKHIFYREQLGVQLRRMGRDTEALAVFRQNVADGKATDISTVN
                     IRLLERKLAKPPAP"
     gene            complement(2073..3020)
                     /locus_tag="PKI64_09285"
     CDS             complement(2073..3020)
                     /locus_tag="PKI64_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOE01229.1"
                     /translation="MMRMFRTVLAMGCLGALVAGCAAPAPSQKIYNRYEALLASRNQM
                     LAKQQPATTAPAPVAEKPAEIAAPPAKPAPAPAPPKPVAPEAPRKPAAPAPAPAKPAP
                     AQPAPPVQVVLPPAEPAASPVQPAPPAPETRPSPAPETPVAPPPAPVTEEPRTEAGYV
                     LKVGDGVQVYLRGIPTPEVIEDVIDEYGMITLPFINDIRAAGLPASELERSIRQIYLD
                     QDIYRNVTVNVVVPTRFYFILGEVRGPGRFQIVSATRVSQAIAAAGGYTEFASGKVQV
                     KRNGRIFKVIRNARRLERQPEDDILLEPDDVVEVLRSWI"
     gene            complement(3040..5154)
                     /locus_tag="PKI64_09290"
     CDS             complement(3040..5154)
                     /locus_tag="PKI64_09290"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOE01230.1"
                     /translation="MAEESQTTALHFLDYWRVIRDRKEVILAVALLTIVSGTAYTLMM
                     PKKYTALTQIQVREDAMDVDPFYQRDINRMGYNPFFLMTQEKILKSKPVLTTVIRNLN
                     LQKVWGAQLNEDKSAITPELALQILQRSIRVEQDRDTMLMNISATSEDPKQAAAIANE
                     IANVYRERRLADKRREIQHAIDAMSNEVRKQQERVEEAEAELERIRQERGIALIGRGL
                     MSIRVESTRLNALENERSAARVDMLVRKARMDQLEDLEGDKLMDAAAYIVQDPTLLSI
                     RQQYIDSQVSLQLMLKNGVVGENHPDVLRLRGAQDELRRQLDSALAGLKAGVKADYEV
                     ARQKYEALDSELASIKATDIESESENYLPFDRAERNVAVNRDILTALRARITQTGIEV
                     EVPRTTVDIVEEADPPDRPSSPLVVLNIFLSVVLGLMAGTGLAFFVEYLDVSIKTVDE
                     VEKYLGLPVLAVIPQQSRPLTEAGQSSGQGEAYRSLRTSMALLGREDNKKIFAVISGG
                     VGEGKSTTLFNLAYVCAEQGSKVLVIDSDLRRPVQHKMVGLANRAGLVNVLTGELKPE
                     DVIQETGVPNLWMLTSGRLRRGTIGIMNNTRLRNLLDHLKEQYDYILLDSPPILGVTD
                     AAILASEVDGVLLVVQYRKYPKIISLRAKRMIENAGGQVLGAVLNNINIMRDDYYYYY
                     HYTTRKYYGVVRHENDDNLTES"
     gene            complement(5267..6481)
                     /locus_tag="PKI64_09295"
     CDS             complement(5267..6481)
                     /locus_tag="PKI64_09295"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOE01231.1"
                     /translation="MKQIRIAMIMGCSLLVAALAASAESPVHLNNRLRLGFDDNVYQA
                     GTATLIDGTTVHHRKTDSFRVLEELELLVNMNQPRTYLGLRYRPSFIWYSDRDEDSTD
                     FLNDLDLNFTHNFTPNLQLALSDTLRASQLPELQEDNYIVRGMDDNYYNSAIATLAIS
                     LRPETRLDISGRYITLIYDSDSDAKKNGDYYSLVGGATLRQQLASRTTLMGDFRYQTV
                     VYNEADAAHNRDSDSVFGGLGIEQTVSSKLTASLRSGVEQRMYDSDQYDDNTAPYVDL
                     SLTFLPSPATRITAATSYSIYESDIANYLSQDRTYVSLSLAHDFTAKLSFYISGAYSL
                     NQYENDYSLNPRFGDYDENSYLVSTRLAYRVNRINWVEIGYQYVKLDSDVPGRENYDR
                     NRADIGWKIQLF"
     gene            complement(6527..7123)
                     /locus_tag="PKI64_09300"
     CDS             complement(6527..7123)
                     /locus_tag="PKI64_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01232.1"
                     /translation="MKKILLYVMIGLMMTATVGMAQGRGKGKAEQDQASGPVTHPQLA
                     ELLVRTLGLLRFLPAAPTSQQLFDILMQNGIVPEKGWELSAVVSKGDLARVLVQAMKA
                     HDMVENPNDPQAWIDALKSLGISLDRLSETIQSVETLPNPMGQDITVQSADPLIFAQN
                     FTPGGTIQYTVDLNLVTRVFTELQMINGEFRPIRPTPH"
BASE COUNT         1392 a         2182 c         2329 g         1417 t
ORIGIN      
        1 acggccccca tagaagggct ggaacggtcg cagaccttcc agccgttctc ccggcgggaa
       61 ggtttcgttc ctcaaccatt ctcgccctac aaaaacagcc gggttgattg ttggctgaaa
      121 cgggcgccga gtggttcagg gcgccggggg cttggccagt ttgcgttcca acagccggat
      181 attcaccgtg ctgatgtccg tggccttgcc atcggccaca ttctggcgga acaccgccag
      241 cgcttcggta tcgcgtccca tgcggcgcaa ttgcacgccc aattgttcgc ggtagaaaat
      301 gtgcttgtgc tgataggcgg cggcgcgacg gaactcggcg agcgctcctt ccggatcgtc
      361 gagcagatta tgaaccaaac ccagcgtgta caccacggcc atgtcgccgg gattcaattc
      421 cgccgcgcgc cgcaagtgca ccgccgcttc cgtggcgagc tcctcacggc cggccttctc
      481 cgcggcttcg tccgggtcgc gataccagaa ggcctgaatc tggcgcagca tgccgaggcc
      541 ggcatgtggt tgccagttgc gcgcatccca gcggctggcg cgcgcgtaat cggcggccgc
      601 ggcgtcccag ttgaggctgc tgcgggcgat atcaccgcgc aaattccaat aataagacag
      661 gccggccgca gtggcgcgcc acgcggcaca caagcagacc aagaccgccc cgccggccac
      721 ggccatgcgc cagccgcggg cctgcgggcg ggcttttttc cgccagcctt cttccagaaa
      781 ccacgcgccc caggccacgc caatcagcca ggccagcacg tgcgggttgg ggaaaatgtg
      841 gaagttgaaa tcgaacaatg cgtgcaccat gttgccggcg acggcgccgg cggcggccgc
      901 cagtaaaaca cgtgtgccgg ctgactgcgc gcggagaaag gccaccagca agccgatgcc
      961 gccccacaac aagcccgcca ccagcaggcc gctgccgatg gcgccatatt ccacttgcaa
     1021 ttgcagatat tcgttgtgga ggtaatccca cagcaggtgg tatttgacgt gcttttggta
     1081 gggcggatag gcccagacaa acgagccgcc gctgaacccg gtgatggacc gttcgcgaat
     1141 catcacaagg gaatcgcgcc acatatccag tcgcccccct ccggcctcga ggccgcgctc
     1201 cagcactggt tgcacacgct cctggacaag cggcagagtg cgccaagcca cccagccccc
     1261 gcccgcggcc aagagcggaa gggccaccag cgccagcgcg aacagcgcgc ggctcttgcg
     1321 ccacgccagc aacagcaggc tggtgatgca accgaccagt aggccggccc aagcgctgcg
     1381 gctctgcgtc cagtacagca ccggggtggc cacgcccaga aaataaagcc ccatcaggcg
     1441 caacgggaaa ccagcatcgg cgcagaacag caacaaaatc gccagcggga acagggtggc
     1501 cagcacgttg gccaaatgat tcgggcagag atatgtgccg ctggcgcgca atccatactg
     1561 ctccgggcgc ggcgcccaga gtacctgccg cgagccgccg acacgctgga caatggcata
     1621 gaggcagatc agcgccaccg acagcagcag gatgaacacc agcacccgcc agcggcgcgg
     1681 ctgattggcc agttgggtcc aactccacaa ggcgccgagc aggcaaagcc atttcaaagc
     1741 ttcccagcgc gcggcataag gcaccatggc gctaggcgcc agcgcgacca cataggtggt
     1801 caaggcggca aaaatccacc acccggccgg ccagcgccag gcgggagtat cggcaaacca
     1861 cagcgggcgg gatgctgcca gcgcaatgcc gcagaaggcc agcacgagac cgggggcaaa
     1921 agcccacgtg cgagtagagc ccaagagcca catgccgccc gtggcgggcc acatcaacag
     1981 gaggagcgtc agcgcgtcat accagcggcg cgcggaggag tggatcactt tcccatcggc
     2041 gaactgcgtc atgggaggct tgccggtcgg catcaaatcc agctacgcag cacttccacc
     2101 acgtcgtcgg gttccagcag gatatcatct tccggctggc gctccaggcg gcgggcgttg
     2161 cgaatgacct tgaaaatgcg gccattgcgc ttgacctgca ccttgccgct ggcaaattcc
     2221 gtatagccgc cggcggctgc gatggcctgc gaaacgcgcg tggcggagac gatttggaaa
     2281 cgcccggggc cgcggacttc gcccaaaatg aagtagaagc gggtgggcac caccacattc
     2341 accgtgacgt tgcggtagat gtcctgatcg agataaatct ggcggatgga ccgctcgagt
     2401 tcggaagccg gcaggccggc ggcgcgaata tcgttgatga acggcagcgt gatcatgcca
     2461 tattcatcaa tgacatcctc aatgacctcc ggcgtgggga taccgcgcaa atagacctgc
     2521 acaccatcac ccaccttgag gacgtagccg gcttcggttc ttggttcttc cgtgaccggg
     2581 gccggtggcg gggcaaccgg tgtttcgggg gcggggctgg gacgggtttc cggcgcgggg
     2641 ggtgcgggtt gcacgggcga agcggccggt tcggcaggag gcaaaactac ttgcacgggc
     2701 ggcgccggtt gagcgggcgc gggcttggcc ggcgctgggg cgggtgccgc cggtttgcgc
     2761 ggagcttcgg gggcaaccgg cttgggcggc gctggagcgg gggccggttt agccgggggt
     2821 gccgcaattt ccgccggctt ttccgcaacc ggcgcgggcg cagtcgttgc gggctgctgc
     2881 ttagccagca tttggttgcg cgaagccaac aaggcctcgt aacggttata gattttttgc
     2941 gaaggcgcgg gagccgcaca gccggcgacc agcgcgccga ggcagcccat cgccaaaacc
     3001 gtgcggaaca tccgcatcat ggaacgatcc gctttcacat caggattccg tgaggttgtc
     3061 atcattttca tgcctgacaa caccatagta ttttctggtt gtgtagtggt agtaataata
     3121 atagtcgtcg cgcatgatgt tgatgttgtt caacaccgcg cccaggacct ggccgccggc
     3181 gttttcgatc atgcgcttgg cgcgcagcga aatgatcttg ggatatttgc ggtactgcac
     3241 cacgagcagt acgccgtcca cttcgctggc gagaatggcc gcgtcggtca caccaagaat
     3301 cgggggcgaa tccagcaaaa tgtagtcgta ctgctccttg aggtggtcca gcaggttccg
     3361 caagcgcgtg ttgttcatga tgccgatggt gccgcggcgg agccggccgc tggtcagcat
     3421 ccacaagttg ggcacgccgg tttcctggat tacatcctcg ggcttgagct cgccggtaag
     3481 cacgttgacc agtcccgcgc ggttggccaa gcccaccatc ttgtgctgta cggggcggcg
     3541 caagtcggag tcaataacca gcactttgct gccttgctcg gcgcaaacgt aggccaggtt
     3601 gaacaacgtg gtggatttgc cttcgccgac gccgccgctg atgacggcaa agattttctt
     3661 gttatcctcg cggcccagca acgccatgct ggtccgcagg gagcgatagg cctcgccttg
     3721 gccgcttgac tggccagcct cagttagcgg gcgggattgc tgcggaatca ccgccagcac
     3781 tggcaggccg agatacttct cgacttcgtc cacggtcttg atggacacat caagatattc
     3841 aacaaagaaa gcaaggccgg tgccggccat caggcccagc accacactca ggaagatgtt
     3901 cagtacgacc agtgggctgg aggggcggtc ggggggatcg gcttcttcca cgatatccac
     3961 cgtggtgcgt ggcacttcca cttcaatacc ggtttgagtg atacgggcac gcagggccgt
     4021 cagaatatca cgattcaccg ccacgttccg ctccgcgcgg tcaaagggca gataattttc
     4081 gctttcggat tcgatatcgg tcgccttgat actggccagc tcgctgtcca gcgcttcgta
     4141 cttctggcgg gcgacttcgt aatccgcttt gacgccggcc ttgagcccgg cgagggcgga
     4201 atccaactga cggcgcaact cgtcttgcgc gccgcgcaag cgcagaacat ccgggtgatt
     4261 ctcgccgacc acgccgttct ttagcatgag ttgcaggctg acctgactgt caatgtattg
     4321 ctggcggatg ctgagcagcg tggggtcttg gacaatgtag gcggcggcat ccatcagttt
     4381 gtcgccttcc aaatcctcca actgatccat gcgcgccttg cgcacaagca tgtccacccg
     4441 ggcggcgctg cgctcgtttt ccagcgcatt caggcgggtg ctttccaccc ggatgctcat
     4501 gaggccgcgg ccgatgaggg caatgccgcg ctcctggcgg attcgttcga gctcggcttc
     4561 cgcctcctcc acgcgctcct gctgcttgcg cacctcattg ctcatagcgt caatggcgtg
     4621 ctggatttcg cggcgtttgt cggcgaggcg ccgctcgcga tagacattgg caatttcatt
     4681 ggcgatggcc gcggcctgtt tgggatcctc gctggtggcg ctgatgttca tcagcatggt
     4741 gtcgcggtcc tgttcgacgc ggatgctacg ctgcaggatt tgcagggcca gttccggcgt
     4801 gatggccgac ttgtcctcgt tgagttgcgc gccccacacc ttctgcaggt tcaggttgcg
     4861 gatcacggtg gtgagcaccg gcttggactt caggattttt tcctgcgtca tcaggaagaa
     4921 cgggttgtag cccatgcggt tgatatcccg ctggtaaaac gggtccacat ccatggcatc
     4981 ttcgcgcacc tgaatctggg tgagcgcggt atatttcttc ggcatcatca gcgtgtaggc
     5041 cgtaccggac acgatggtca gcagcgccac cgccagaatg acctccttgc gatcgcggat
     5101 gacccgccaa taatcgagga agtgtaacgc agttgtttgg gattcttcgg ccatgtcttg
     5161 ttatcctgaa cattcgggcg ggatgacccg cccgcgccgg tgtgaccggc atacgtgaac
     5221 atagaggagg cgaccgttgc cggtcgcctg cctcgcccgc gaaggactag aacagttgga
     5281 tcttccagcc aatgtccgcg cggttccggt cgtagttttc gcggcccggc acatcgctgt
     5341 cgagcttcac atactggtaa ccgatttcca cccagttgat gcgattgacc cggtatgcca
     5401 agcgcgtgct gacgaggtag gagttctcgt cgtagtcgcc gaaccgcggg ttgagggagt
     5461 aatcgttttc gtattggttc agcgagtagg cgccgctgat gtagaagctc aacttcgcgg
     5521 tgaagtcgtg ggccaagctg agggagacat aggtgcggtc ctggctgagg tagttggcaa
     5581 tgtccgactc atagatggag taggacgtgg ccgcggtgat gcgggtggcc gggctcggca
     5641 ggaaggtcag ggacaggtca acatacggcg cggtgttatc gtcgtactgg tcgctgtcgt
     5701 acatgcgctg ttccacaccg gaacggaggc tggcagtcag cttggagctg acggtctgtt
     5761 cgatgcccaa gccgccgaac acagagtcac tgtcgcggtt atgggccgca tccgcctcgt
     5821 tatacaccac ggtctggtag cggaagtcgc ccatgagggt cgtgcggctg gccaattgct
     5881 ggcgcagggt ggcaccgccc accaggctgt agtaatcgcc gtttttcttg gcatcggaat
     5941 cggaatcata aatcagcgtg atgtagcgac cggagatatc caagcgggtt tccgggcgca
     6001 ggctgatggc cagcgtggcg atggcggagt tgtagtagtt gtcatccatg ccgcggacga
     6061 tgtagttgtc ttcctgcaat tccggcaact ggctggcgcg gagcgtgtcg ctcaaggcca
     6121 actgcaagtt gggcgtgaaa ttgtgcgtga agttcaggtc cagatcgttc aggaagtcgg
     6181 tgctgtcctc gtcacgatcg gaataccaga tgaacgacgg gcggtagcgc aggcccaagt
     6241 aggtccgggg ctgattcatg ttcaccagca gctcgagctc ttccagcacg cggaagctgt
     6301 ccgtcttccg gtggtggacc gtggtgccgt caatcaaggt tgccgtgcca gcctgataga
     6361 cgttgtcgtc gaagcccaag cgcaggcggt tgttgaggtg cacgggcgat tccgcgctgg
     6421 ccgccagcgc cgccaccaac aagctgcagc ccattatcat cgcaattcgg atttgtttca
     6481 tggccatatt tccttgtaag ggttgacagt ttcttgcggg ttgcaattag tgaggggtgg
     6541 gccggatggg gcggaactcg ccattgatca tttgcagttc ggtgaacacg cgggtcacga
     6601 ggttcaggtc caccgtgtat tgaatggtgc cgccgggcgt gaagttctgg gcgaaaatca
     6661 aggggtcggc ggactgcacg gtgatatcct ggcccatcgg gttgggcagg gtttccacgc
     6721 tttggatcgt ctcgctcagg cggtcgaggg aaattccaag gctcttcagc gcgtcaatcc
     6781 acgcctgcgg atcgttgggg ttttcgacca tatcgtgggc cttcatggcc tgcacgagca
     6841 cgcgggcgag atcgcccttg ctgaccacgg cggagagctc ccagcccttt tccggcacga
     6901 tgccgttctg catcaagatg tcaaagagtt gctgggaagt gggcgcggcc ggcaggaagc
     6961 gcagcaggcc aagagtccgc accagcagtt cagccaattg gggatgggtc acggggccgg
     7021 aggcttgatc ctgctccgct ttgcccttgc cccgaccttg ggccatgccg accgtggcgg
     7081 tcatcatcaa cccgatcatt acatacaaca gtattttttt catcgttcgc tctccttgcc
     7141 caagggcggc agcattttct aaaccggacc cgtcagggcc gactactcac cgtaggagga
     7201 taatgcccca ttgccccggg caacgtcaac agcaaaaccg aaaaaaattc cgcagaatac
     7261 gccccggtca attgacaaag taaatgcacc ccccagggtc tcttgggggt gcgtttagtc
//
LOCUS       DAOPNP010000115         7318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_11274_length_7318_cov_10.411538, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000115 DAOPNP010000000
VERSION     DAOPNP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11274_length_7318_cov_10.411538"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKI64_09305"
     CDS             <1..342
                     /locus_tag="PKI64_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE01233.1"
                     /translation="AHFKRNYIHVRDVARAFLHALDHFDAMRGQPYNVGLSDANLSKL
                     ELCQEIQKQVPDFTIMEAPIGKDPDQRNYIVSNAKIEATGYRPQVSIQRAIAELIKGY
                     QIIRRNQHANV"
     gene            686..3139
                     /locus_tag="PKI64_09310"
     CDS             686..3139
                     /locus_tag="PKI64_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOE01234.1"
                     /translation="MWKNPARAAPTFPQRGNIFSTAWKSWLALLWLGPAAVLANDTLE
                     SFAPATAAAWKPYLDSPAVSATDRALAFPTPFTKGNDRFAWDKTIAADLSAAVAFEVD
                     VACPNPEALRALGLYFRSGAGWYVASKPLTRAGPQTLTFAKSDFSTEGQPAGWHKIDG
                     IRLSPWKGARAADTALVFSRLGRVTGSALVVVKPTSSCPDAATRAVAEKTAARVSSLL
                     IEAGIGHRLLTDDEVAKGALSSATVALLPYNPQPGAAELKALQAFLARGGKLGVFYGA
                     SPELAAAMGFKLGPYLKAERPDRWRSIVFTDVRGGFLAPRIWQNSTSLMPAYPARADA
                     AVVAAWHNARGVLQPEPAVVISPHGFWMAHILQSDDLPSKRDMLVALCAQMDPGLWAP
                     AARRAVQEAGQINDYPNLAAAVAGIGRQANQAAQPAEIQSLLATATSLSQQAQTALAA
                     GQFTNALLLAKRQRQFLLRGDAAVQLPRRGEFVGVWDHDGTGFVPGDWPTTATTLAAH
                     GVNAIFANVAWGGCAHYPSKYLPASNTLRLYGDQIAAGLAAAQARGLQYHVWLVLGKL
                     DGAPADFVARMKKEGRLQVTDTGVTRPWLSPHHPANRALLLAVVGEIARNYPTIAGIH
                     LDYIRLPDSASCYSATTRARFEADTRKKCAAWPAEVKVGGKRHAEFRKWRTADITALV
                     RDVRSTLRRANPNAKLSAAVYGIAAPDGGNIAQYWPDWLRAGTVDFLVPMNYTESPAE
                     FANWLRTQQSYPGAKGKIIPGIGVTADESRLDPAQVVRQVVLARQAGCPGFVLFALSG
                     TLREETLPALRQGITRPIP"
     gene            complement(3152..6955)
                     /locus_tag="PKI64_09315"
     CDS             complement(3152..6955)
                     /locus_tag="PKI64_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE01235.1"
                     /translation="MKRIIRLAVGLCGVGVLSAWGGAPLILDWGTVDTSSAPQQQQTR
                     AIRRATPAVQQALTAQGKAPWLVQCDDVIHEEWKDSLVAAGATLVGYMPENAYLVEAT
                     PAQMTAIAALPQVAWVGEYRPEYKRAARVRAKLAKVAGGEETDAAMAYRVLLFSDDYL
                     ADVTERIAALTGRAVSVAEGTLIRTDLTAEQIETITGWGEVSWVEPYTRPRLWNNVAT
                     GPAMMNVSNVWSGLGLTGAGQTIAVCDTGLDTGNTGTVHPDFAGRVTGFGWEDGVYSA
                     SYSWADTDSHGTHVCGSVLGDGTMSSGLYKGAAYEANLIIQGAQEDLSGIPATLANLF
                     RQAYTNGARIHSDSWGYDDHGYYNSDSRGLDQYVWSNKNFLVLVAAGNSGTDSNTMNG
                     VIEPMSVASPATAKNCLTVGAAENYRLSGGYSTMKWGSAWPDDYPAAPIKDDYISRPM
                     SNNVQGMAAFSSRGPCNDGRIKPDLVAPGTDIISTRSRRSSDTGWGTVSGNTNYLYMG
                     GTSMATPLMAGAAGLARQWVTTTGGVTNPSAALLKALLITGARDMTPGQYLSGTKQEI
                     FPRPDKSQGWGHVNLYNALKPPTNLFLALIDTNSLTTGKTNTFTYTVSSGSTNRFIAT
                     LAYADYWGSVGSGKQLVNDLDLTVQKPSGTTVYPNNLGHADATNNVELIDLEADEAGT
                     YTVRVVGRNVPSGGSQPYALVVRGPKADETPTAPIFGANPGPLSTPAGVMIDFTVTAS
                     GYPAPVIALKSTTAAADSYDVDNGYCIYEPPLGDVGTQTFTFTASNSQGVATQVVSVT
                     VQTPPPPAPAAIWASATNSANFTAAWSAAAGATSYRLDVSTQQSFATGGGGGGGTNIA
                     ENIQSWTARASYGSWTQSISAGNVTMADCIVSPNAAASGVGSAGRVQLKKTTGVLELP
                     PVNTADTITMNLAAGAASRTVKLQKYNGGSWTDLTTWSGINTTGAAFSFNVNDSGSAV
                     RLRLASPSDPVYVHDIIVTALGGESSPSYVPGYSNRTVSGTSQSVTGLTANTTYYFRA
                     RAVNSGGTSGNSPTGSVTTLAPGTTPTMVAIPPQTTYVGGELEYRVSATEPDGDTLTF
                     ACTSAVSASTWEFDAQTGDFLFQPLLAQKGTNSFTFTASDKDGTSAPAIMTVKVYSAA
                     ASNAFTQWVEDQNQNPASPNFAPNADFDGDGLTTYEEYLADTDPASAGSVLKLEGVDT
                     AVQRVTFPASSNRYYQIVYRTDITNAAATFTNNLGWGVPGMVITNTAPNAWFGIIRAL
                     LNAPN"
BASE COUNT         1282 a         2393 c         2312 g         1331 t
ORIGIN      
        1 gcccacttca agcgcaacta catccacgtc cgcgacgtcg cccgcgcctt cctccacgcc
       61 ctcgaccatt tcgacgccat gcgcggccag ccctacaacg tcggcctctc cgacgccaac
      121 ctctccaagc tggagctgtg ccaggaaatc cagaaacagg tgcccgactt caccatcatg
      181 gaagcgccca tcggcaagga ccccgaccag cgcaactaca tcgtcagcaa cgccaaaatc
      241 gaagccaccg gctaccgccc gcaagtttcg atccagcgcg ccatcgccga actcatcaag
      301 ggctaccaga tcattcggcg caaccagcac gccaatgtct aaggcggccg cgccccaccc
      361 ccttttccac actgtggaaa gtttttttcc acagcgtgga aaaatcgcgc aaagtttgcc
      421 gcttcgctcg ctatggctcg ctcgctcgcc cggctgcgcc ggccgctata gagctcgcgt
      481 tcgcccatcg ccctgcgggc tcgggctcgg cactcgccta ttttccattg tgtggaaaaa
      541 ttcgccaaag tttttccatt gcgtggaaaa attgccgaaa ctttttccat tgtgtggaaa
      601 aatcgcccaa aggttttcca tagcgtggaa aaaagcgacg cggtgggccc aagtttttcc
      661 acagtgtgga aaactttttt ccacagtgtg gaaaaatccg gcgcgcgcgg cccccacttt
      721 tccacagcgt ggaaatattt tttccacagc gtggaaaagt tggctggcgc tgctgtggct
      781 gggacccgcc gcggtgttgg ccaacgacac gctggagtcg ttcgcccccg ccaccgccgc
      841 ggcatggaag ccctatctgg actcgccggc ggtcagcgcc acggaccggg cgctcgcctt
      901 ccccaccccg ttcaccaagg gcaacgaccg tttcgcgtgg gataaaacca tcgccgccga
      961 cttgtccgcc gccgtcgcgt tcgaggtgga tgtggcctgc cccaaccccg aggcgctgcg
     1021 cgcgctgggg ctttatttcc gcagcggcgc gggctggtat gtggccagta agccgttgac
     1081 gcgcgccggg ccgcaaaccc tcacctttgc caaaagcgat ttctccaccg agggccagcc
     1141 cgccggctgg cacaagattg acggcatccg cctctcgccg tggaagggcg cccgcgcggc
     1201 ggataccgcg ctggtgttca gccgcttggg ccgcgtgacg ggctcggcgc tggtggtcgt
     1261 gaagcccacc agctcctgcc ccgatgccgc cacgcgcgcc gtggccgaaa aaacagccgc
     1321 gcgcgtgagc agcctgctga ttgaagcggg catcggccac cggttgctga ccgatgacga
     1381 ggtggccaag ggcgcgctga gttccgccac ggtggcgctg ctgccctata acccgcagcc
     1441 cggcgcggcg gagctgaagg cgctccaagc gttcctggcg cgcggtggca agctgggcgt
     1501 gttttatggc gcatcgccgg agctggcggc ggccatgggc ttcaagctgg ggccatacct
     1561 caaggcggag cgccctgacc gctggcggtc cattgtgttc accgatgtgc gcggcggatt
     1621 ccttgcgccg cgaatttggc aaaactccac cagcctcatg ccggcctatc ccgcgcgcgc
     1681 ggatgcagcg gtggtggcgg catggcacaa tgcgcgcggc gtgctccagc cggagccggc
     1741 ggtggtgatc tcgccgcacg gtttctggat ggcgcacatt ttgcaaagcg atgatctccc
     1801 ctccaagcgc gacatgctcg tggcgctctg cgcccaaatg gaccccggcc tgtgggcgcc
     1861 tgccgcgcgc cgcgccgtgc aagaagccgg gcaaatcaac gactatccca acctggccgc
     1921 cgcggtggcg ggcatcgggc ggcaggcaaa ccaggccgcg cagccggcgg aaatccagtc
     1981 gctgctggct acggccacca gcctgagcca acaggcgcag accgcgctgg cggccgggca
     2041 gttcaccaac gcgctactgt tggccaagcg ccaacggcag ttcttgctgc gcggcgatgc
     2101 cgccgtgcaa ctgcctcggc gtggtgagtt cgtcggcgtg tgggatcacg acggcaccgg
     2161 cttcgtgccc ggcgactggc ccactacggc gaccacgctg gccgcgcacg gcgtcaacgc
     2221 catctttgcc aatgtggcgt ggggcggctg cgcgcattac ccatccaaat acctgccggc
     2281 atcgaacacc ctgcggctct atggcgacca aatcgccgcg gggctcgccg ccgcgcaggc
     2341 gcgtgggctg cagtatcacg tctggctcgt gctgggcaaa ctggatggtg cgcccgccga
     2401 ctttgtggcg cgcatgaaaa aagagggtcg gctgcaagtg actgataccg gcgtcacgcg
     2461 gccgtggctc agcccgcacc acccggccaa ccgcgcgctg ctactggcgg tggtgggcga
     2521 gattgcccgc aactatccga ccattgccgg cattcatttg gattacatcc ggctgccgga
     2581 ttccgccagt tgctactccg ccaccacgcg cgcgcgtttc gaggccgaca cccgcaagaa
     2641 gtgcgccgcg tggcccgccg aggtcaaggt cggcggcaag cgccacgcgg aattccgcaa
     2701 gtggcgcacg gccgacatca ctgcgctggt gcgcgatgtt cgttccacgc tgcgccgcgc
     2761 caatcccaac gccaagctct ccgccgcggt gtacggcatc gccgcacccg atggcggcaa
     2821 catcgcccag tattggcccg attggctgcg cgccggcacc gtggatttcc tggtgccgat
     2881 gaattatacc gagtcgcccg ccgagtttgc caactggctg cgcacgcaac agagctaccc
     2941 cggcgccaaa ggcaaaatca tccccggcat cggcgtcacc gccgatgaat cccggctgga
     3001 tcctgcgcag gttgtgcggc aagtggtcct ggcgcggcag gccggatgcc ccggtttcgt
     3061 actgtttgcg ctctccggca ccctgcgcga ggaaacgctc cccgcgctgc ggcagggcat
     3121 cacccgcccg atcccgtagg atggcggggc atcaattcgg cgcgttgagc aacgcgcgga
     3181 tgatcccgaa ccacgcgttg ggcgcggtgt tggtgatgac catgccgggc acgccccacc
     3241 ccagattgtt cgtgaaggtg gcggcggcgt tggtgatgtc agttcggtac acgatctggt
     3301 aatagcggtt cgagctggcg gggaaggtga cgcgctgcac ggcggtgtcc acgccttcca
     3361 gcttcagcac gctcccggcc gatgccggat ccgtgtcggc cagatattcc tcataggtgg
     3421 tcaggccgtc accgtcaaaa tccgcgttcg gggcgaagtt cgggctggcg gggttttgat
     3481 tttggtcttc cacccattgc gtgaaggcgt tgctggccgc ggcggaatag accttgacgg
     3541 tcatgatggc cggcgcgctg gtgccatcct tgtcgctggc ggtgaaggtg aaggagttgg
     3601 tccccttctg tgcgagaagc ggctggaaga ggaaatcgcc cgtctgggca tcaaattccc
     3661 acgtgctggc gctcacggcg gaggtgcagg caaaggtcag cgtgtcacca tccggctccg
     3721 tagcgctgac gcggtattcc agttcgccgc ccacataggt ggtttgcggc ggaatggcca
     3781 ccatggtggg cgtggtgccc ggcgccagcg tggtgacgct gccggtgggc gaattgccgc
     3841 tggtgccgcc gctgttcacc gcgcgcgcgc ggaaataata agtggtgttg gccgtcaacc
     3901 cggtcacact ctggctcgtc cccgaaaccg tccggttgga atagccgggg acgtaagagg
     3961 gagaggattc tccccccaac gcggtcacga taatgtcgtg aacgtaaacg ggatcactgg
     4021 gcgaggcgag gcgcaaccgg accgcagatc ccgaatcatt gacgttgaac gagaaggccg
     4081 cgcctgttgt gttgatcccg gaccacgtgg tgagatccgt ccaactgcca ccattatatt
     4141 tttgcagctt cacggtgcgg ctcgcagcac cggccgccag attcatggtg atggtgtcgg
     4201 ccgtgttcac tggcggcaac tccaagacac cggtggtctt tttgagttgg acgcgccccg
     4261 cgctgcctac accgctggcc gcggcgttgg gcgacacaat gcaatccgcc atggtgacgt
     4321 ttccagccga aatgctttgc gtccagcttc cgtaactcgc gcgggccgtc cagctctgaa
     4381 tgttttcggc aatgttcgtg cccccgccgc caccgccccc ggtggcgaag ctttgttggg
     4441 tgctgacatc cagccggtag ctggtggcgc cggcggcggc gctccaagcc gcggtgaagt
     4501 tggcgctgtt ggtggcgctg gcccaaattg cagccggcgc gggcggcggc ggagtttgca
     4561 ccgtgacgct taccacctgc gtggccacgc cctgcgaatt actggcggtg aacgtgaagg
     4621 tttgcgtgcc gacatcgcca agcggcggct catagatgca gtacccattg tcaacgtcgt
     4681 atgaatcggc ggcggcggtg gtgcttttca gcgcgatcac cggcgcggga tagccgctgg
     4741 cagtcacggt gaaatcaatc attacgccgg cgggggtgga cagcgggccg gggttggcgc
     4801 cgaagatcgg cgcagtgggg gtttcatcgg ccttgggccc gcgtaccacc agtgcgtagg
     4861 gctggctgcc gccagaagga acgttgcgcc cgactacccg cacggtgtag gtcccggctt
     4921 cgtccgcctc cagatcaatc agctcgacgt tgttggtggc atccgcgtga cccagattgt
     4981 tcggatagac tgtcgtgccg gagggcttct ggacggtcag gtccaaatcg ttcaccagtt
     5041 gtttgccgct gccgacggag ccccaataat cggcataggc cagggtggcg atgaaccggt
     5101 tggtcgagcc gctgctgacc gtgtaggtga aggtgttggt tttgccggtg gtcaaactgt
     5161 tggtgtcaat aagcgcaagg aacaggttgg tcggcggctt gagcgcgtta tacaagttca
     5221 cgtgtcccca gccctgcgac ttgtccgggc gcgggaaaat ttcctgcttg gtgcccgaaa
     5281 gatattgacc gggcgtcatg tcgcgcgcac cggtaatcag cagcgccttg agcagggccg
     5341 cgctgggatt ggtgacgccg ccggtggtcg tgacccactg gcgcgccaag ccggcggcgc
     5401 cagccatcag cggcgtggcc atgctggtgc cgcccatgta tagataattg gtgttgccgg
     5461 aaaccgtgcc ccagccggta tcgctgctgc gccgcgagcg ggtggagatg atatcggtgc
     5521 ccggcgcgac gaggtcgggt ttgatgcggc catcgttgca cgggccgcgg ctcgaaaatg
     5581 ccgccatgcc ttgcacgttg ttgctcatgg gccgggaaat atagtcatcc ttgatgggcg
     5641 ccgcgggata atcatccggc cacgcactgc cccacttcat ggtggaatag ccacccgaga
     5701 ggcgataatt ctccgccgcg cccacggtca ggcaattctt ggcggtggcg ggcgaggcca
     5761 cggacatcgg ttcaatcacc ccgttcatcg tattggaatc cgtgccggaa ttgccggccg
     5821 ccaccagcac gaggaagttc ttgttgctcc acacgtattg atccagcccg cgcgaatccg
     5881 aattataata gccgtgatca tcatagcccc agctatccga gtgaatgcgt gcgccgttag
     5941 tgtaggcctg cctaaacaaa ttggcgagag tggcgggaat gccggacaga tcttcttgcg
     6001 caccctgaat gatcaagttc gcttcgtagg ctgccccttt gtagagacca gacgacattg
     6061 tgccatcgcc cagcacggag ccgcacacgt gtgtgccgtg cgaatcggta tctgcccaac
     6121 tataactcgc gctatagacg ccatcttccc acccaaaacc tgtcacccgg ccggcaaaat
     6181 ccggatggac ggtgccggtg ttgccggtat cgaggccagt atcgcaaacg gcaatcgtct
     6241 gtcccgcgcc ggtcaggccc aacccgctcc acacgttcga gacgttcatc atggcggggc
     6301 cggttgccac gttgttccag agccgcgggc gggtataggg ctccacccag ctcacttcgc
     6361 cccagccggt gatcgtctca atttgctcgg cggtcaggtc ggtgcgaatc agggtgccct
     6421 cggcgacaga caccgcacgg ccggtcagcg cggcgatgcg ctcggtcaca tcggcgagat
     6481 aatcatcgct gaacagcagt acgcggtacg ccatggcggc atctgtttct tcgccgccgg
     6541 ccactttggc cagcttggcc cgcacccgcg ccgcgcgctt gtattccggg cgatattccc
     6601 ccacccacgc cacctgcggc agcgccgcga tggccgtcat ctgcgccggg gtggcttcca
     6661 ccaagtaggc gttttccggc atgtagccca ccaacgtcgc gccggcggca accaacgaat
     6721 ccttccattc ctcgtggata acatcatcgc actgcaccag ccacggcgcc ttgccttgcg
     6781 ccgtcaaggc ctgctgcaca gcgggagtcg cccggcggat ggcacgcgtt tgctgctgct
     6841 gcggcgcgct cgaggtatcc accgtccccc agtccagaat cagcggcgcg cccccccatg
     6901 ccgatagcac gccaacgccg cacaaaccca ctgccaacct gatgatccgt ttcatctgtc
     6961 actccatccg cggcccacgt gccgatacgg acaaacgtac ggcggttgcc ccctcataat
     7021 caaaaaataa cggaggcatc ttttgaaccg atttaccgcc gcccccgccc ccaccgcgcc
     7081 aagccagaaa aggccaaccg ccgcaaggcg gtggcagaag tcagaagtca gaggtcaggg
     7141 gaaaaccaac gagccagccg ccgatgcttt gcggttttct gtagccgggg cggtgacccc
     7201 ggcagcggac tcaacgagcc cgcctacagg acagccaaca gccaccgcaa ggcggtggca
     7261 gaggtcagaa gtcagaggtc aggggaaaac caacgagcca gccgccgatg ctttgcgg
//
LOCUS       DAOPNP010000116         7050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_11807_length_7050_cov_8.392280, whole genome shotgun sequence.
ACCESSION   DAOPNP010000116 DAOPNP010000000
VERSION     DAOPNP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11807_length_7050_cov_8.392280"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PKI64_09320"
     CDS             <1..1241
                     /locus_tag="PKI64_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOE01236.1"
                     /translation="LKGMLVQAKKDGVRRARVHALLDGRDVPPTSALDFVIPFEQFLA
                     DLNADNSVDFAIASGGGRMKVTMDRYEADWRIVERGWKTHVLGQGTLYPSAQAAIEAL
                     RRENPGILDQDLPPFVIGKDGQPIGPIEDNDSVILYNFRGDRAIELTRAFEEDDFAHF
                     DRQRRPKVLFAGMMQYDGDANIPKSFLVEPPRITRTMGEYLADTGVRQLAISETQKFG
                     HVTYFFNGNRAEKFSEELEDYVEVPSDRVPFEQRPWMKAAEITDNVIEAMNSGKYRFI
                     RVNFANGDMVGHTGVFPAVQIAVETVDLCLGRLIPAAQAAGAILVISADHGNSDDMYE
                     HAKDGSVIKDKATGRPKARTAHSLNPVPAIIYDPSNAAGVKLTDKKGLGISSLAATCL
                     NLIGYQTPEGYDPSLLEIGS"
     gene            1476..1763
                     /locus_tag="PKI64_09325"
     CDS             1476..1763
                     /locus_tag="PKI64_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOE01237.1"
                     /translation="MSWLTDTPAGAVLTLRIVPRAAKNAIVGEHGDALKIRLCAPPVD
                     GAANAALIEFLADALALPRSRIQILSGATSRHKRVLLAQASAAAIRSQLQP"
     gene            1828..2922
                     /gene="hypD"
                     /locus_tag="PKI64_09330"
     CDS             1828..2922
                     /gene="hypD"
                     /locus_tag="PKI64_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOE01238.1"
                     /translation="MLHLTEYSNSATIRGLAAEIARISRRPARIMEVCGGQTHAILRH
                     ALDTLLPPHVHLLHGPGCPVCVTPGDYLDHAIAIARQPGTIITTFGDLLRVPSATHGD
                     LFAAKARGADIRMITSPLQALDFAREQPEKQVVHLAIGFETTAPANAMLAYLARERGQ
                     RNVSLLVSQFLVPPILRHISADPANTPDAFLAAGHVCAIMGSAPYEPLARELRRPIAI
                     TGFEPADILLGVLAVIRQLEAGEARMENMYPRVVHPAGNPAAQKIMDTVFQIADREWR
                     GLGMVPQSGLALRPEYADLDAAQRFPVSSSPVAVASNADCPAADVLLGKLEPCDCPHF
                     GTRCTPDHPLGAPMVSPEGPCAAYYLHRHG"
     gene            3057..3929
                     /gene="dapA"
                     /locus_tag="PKI64_09335"
     CDS             3057..3929
                     /gene="dapA"
                     /locus_tag="PKI64_09335"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOE01239.1"
                     /translation="MFQGAYTALITPFQADGKVDFGKLRELVELQVAGGIDGLVPVGT
                     TGESPTLDIDEHLEVIRVVTEAAAGRVKIIAGTGANSTDEALELTRHAKDFGVDGTLQ
                     VTPYYNKPTQAGLVRHFSAVADLGLPVVLYNVPGRCSCEIAVGTIAELAKHPHVVCVK
                     EAGGSVDRVSKIRAACDIEILSGDDALTLPMMAVGASGVISVASNVAPRAVADLVHAA
                     AAGNWDEARRLHYQYYALFTDLFIETNPIPVKAAAEMMGLAPALYRLPLCAMAPANRD
                     KLRATLQQCGLVRA"
     gene            3926..4762
                     /gene="dapB"
                     /locus_tag="PKI64_09340"
     CDS             3926..4762
                     /gene="dapB"
                     /locus_tag="PKI64_09340"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOE01240.1"
                     /translation="MSTPIKILLVGAAGRMGQSITRLLAAGTVPGLELVAAVDRADSP
                     LAGQDAGTAAGTRALGVKITSDLAAALAAKPAVAIDFSAHTLTAAAAPLLAAAGVAWV
                     NGTTGLEAADNAAVAQAAQRIPVVRSANMSLGVNLLYALVEQAARALRGKDYDCEIIE
                     RHHRRKKDAPSGTALYLGEAAAQGYDWPLRDVAVDGRSGLTGARPAKQIGFHAVRGGD
                     IVGDHTVLFAADGECVELAHRATSRDTFALGALRAASWLAGRAPGLYSMRDVLGLNPG
                     SS"
     gene            4759..5271
                     /gene="folK"
                     /locus_tag="PKI64_09345"
     CDS             4759..5271
                     /gene="folK"
                     /locus_tag="PKI64_09345"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOE01241.1"
                     /translation="MNGIEVGLSLGANLDDKLANLRAARTAIAAIPYVSVLASAPLYE
                     TEPVGVPEEFANLKFFNTVVIIGTSLDAHRLFAELQKVELALGRKRTLRPNTPRVIDI
                     DMIYYNGQTIRSGGLVIPHPRWTKRRFVLQPLADVRGYLVLPGHDRRVRDILAALPPG
                     QDIKKIMVEW"
     gene            5274..6065
                     /gene="panB"
                     /locus_tag="PKI64_09350"
     CDS             5274..6065
                     /gene="panB"
                     /locus_tag="PKI64_09350"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOE01242.1"
                     /translation="MRLTPRSITAMKGREKIAALTAYDYRTAQLLDAAGIPLLLVGDS
                     LGTTLLGYENTLPVTLLDMLRHTAAVVRGVKNAVVVVDMPFMTYQASIEQALRNCGRA
                     IQKTGCHAVKIEGGEFRAPTVAALVQNGIPVLGHIGLTPQSVQTLGLRVQGRGTEAAR
                     QLVADAQAIAAAGAFAIVLEAVPSALGAEVTAAVPVPVIGIGAGPGCDGQILVINDLL
                     GLSGDFKPKFVKQYANLGAQISAAASAYKSEVAGAVFPGPEHGYD"
     gene            6095..6946
                     /gene="panC"
                     /locus_tag="PKI64_09355"
     CDS             6095..6946
                     /gene="panC"
                     /locus_tag="PKI64_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOE01243.1"
                     /translation="MKVITHSPEMQQTALALRAAGRRLALVPTMGNLHAGHLALVRLA
                     KQHADVVIVSIFVNPTQFGPNEDFQTYPRTFEADRAQCAAAGVDIIFYPADVSEIYPA
                     DASITVVESALSQTLCGASRPGHFTGVCTVVAKLFNLTLPHVAVFGEKDGQQLRIMRR
                     MARDLHFPVEIIAGPTVREQDGLALSSRNQYLTPAQRAQATCLRRALTEAEQRFAAGE
                     RNPQCLITAMWNLIAAEPDTRIDYIAIVDDETLAPVLEPITRPALVALAVWVGQPRLI
                     DNTVLRP"
BASE COUNT         1252 a         2515 c         2042 g         1241 t
ORIGIN      
        1 acctcaaggg catgcttgtc caggccaaga aggacggcgt caggcgcgcc cgcgtccacg
       61 ccctcctcga cggccgcgac gtccccccca cctccgccct cgatttcgtc atccccttcg
      121 agcaattcct cgccgacctc aacgccgaca actccgtcga cttcgcaatc gcttccggcg
      181 gcggccgcat gaaggtcacc atggaccgct acgaagccga ctggcgcatc gtggaacgcg
      241 gctggaaaac ccacgtcctc ggccaaggca cgctctaccc ctccgcgcag gcggccatcg
      301 aagccctgcg tcgcgaaaac cccggcatcc tcgaccaaga cctcccgccg tttgtcatcg
      361 gcaaggatgg ccagcccatc ggccccatcg aggacaacga ctccgtcatc ctctacaact
      421 tccgcggcga ccgcgccatc gagctgaccc gcgcgtttga ggaagacgac ttcgcgcact
      481 tcgaccgcca gcgccgcccc aaggtgctct ttgccggcat gatgcagtac gatggcgacg
      541 ccaacatccc gaaatcgttt ctcgtcgagc ccccgcgcat cacccgcacc atgggcgaat
      601 atctcgccga caccggcgtc cgccagctcg ccatcagcga gacgcaaaaa ttcggccacg
      661 tcacctactt cttcaacggc aaccgcgccg aaaaattcag cgaagaactc gaggactacg
      721 tcgaagtccc ctccgaccgc gtccccttcg agcagcgccc gtggatgaag gccgctgaaa
      781 tcaccgacaa cgtcatcgag gccatgaact ccggcaagta ccgcttcatc cgcgtcaact
      841 ttgccaacgg cgacatggtc ggtcacaccg gcgttttccc cgccgtgcaa atcgccgtgg
      901 aaaccgtgga cctctgcctg ggccgcctga ttcccgccgc ccaggccgcc ggcgccatcc
      961 tcgtcatcag cgccgaccac ggcaactccg acgacatgta cgagcacgcc aaagatggtt
     1021 ctgtcatcaa ggacaaagcc actggccggc ccaaggcccg caccgcgcac tctttaaacc
     1081 ccgtacccgc catcatctac gaccccagca acgccgccgg cgtcaaactg acggacaaaa
     1141 aaggcttggg catctcgtcg ctcgccgcca cctgcctcaa cctcatcggt taccagactc
     1201 cggaaggcta cgacccttcg ctcctcgaaa tcggcagcta gcccccccat gcggcaacgc
     1261 gcggcccatc ccttttccat ggcgtggaaa aaatatttcc acactgtgga aaaatcggtc
     1321 caaagttgtt ccattgtgtg gaaaaatcgc caaaactttt tccattgcgt ggaaaaatcg
     1381 ccaaaacttt ttccattgcg tggaaaaata tcaccccatt tttccacact gtggaaaaaa
     1441 gatttccaca ctgtggaaaa gtgcgaggcc cgcccgtgag ctggctgacg gacacccccg
     1501 ccggcgcggt gctgaccctg cgcattgtgc cgcgcgccgc caaaaacgcc atcgtgggcg
     1561 aacatggcga cgccctcaaa atccgccttt gcgccccgcc cgtggatggt gccgccaacg
     1621 ccgcgctaat tgagtttctg gccgacgccc tcgccctccc gcgctcccgc atccaaatcc
     1681 tttccggcgc cacctcgcgc cacaagcggg ttctccttgc ccaagcgtcc gctgccgcta
     1741 tccgctctca actccaaccc tgacttctga cctctgactt ctgacctctt tactgaaccc
     1801 tgaaccccga accctgaccc ctcttccatg ctccacctca ccgaatattc caactccgcc
     1861 accatccgcg gccttgccgc ggagatcgcg cgcatctccc ggcgacccgc gcgcatcatg
     1921 gaagtctgcg gcggccaaac ccacgccatt ttgcgccacg cgctggacac gctgctgccg
     1981 ccgcacgtcc acctgctcca cggtcccggc tgcccggtat gcgtcacccc cggtgattac
     2041 ctcgaccacg ccatcgccat cgcgcgccag cctggaacca tcatcaccac cttcggcgat
     2101 ttgctccgcg taccttcggc cacccacggc gacctcttcg ccgccaaggc ccgcggcgcg
     2161 gatatccgca tgatcacctc cccgctgcaa gccttggact tcgcccgcga gcagccggaa
     2221 aaacaagtgg tgcatctcgc cattggtttt gaaaccaccg cgcccgccaa cgccatgctc
     2281 gcttatctgg cgcgcgagcg cggccaacgc aatgtctccc tgctcgtctc gcagtttctg
     2341 gtgcccccca tcctgcgcca catcagcgcc gatccggcca acacccccga tgcttttttg
     2401 gccgccggcc acgtctgcgc catcatgggc tccgctccct acgaaccgct cgcccgcgaa
     2461 ctgcgccgcc ccatcgccat taccggtttt gaacccgccg atattctcct cggtgtcctc
     2521 gccgtcatcc gccaactgga agctggcgaa gcccgcatgg aaaacatgta cccccgcgtg
     2581 gttcatcccg ccggcaatcc cgccgcgcaa aaaatcatgg atacggtatt ccagatcgcc
     2641 gaccgcgaat ggcgcggcct gggcatggtc ccgcagagcg gcctcgccct ccgccccgaa
     2701 tatgccgacc tcgacgctgc ccaacgtttc cctgtctctt catcgccagt cgccgtcgcc
     2761 agcaatgccg actgccccgc cgccgatgtc cttttgggca aactcgaacc ctgcgattgc
     2821 ccgcacttcg gcacgcgctg cacccccgac caccccctcg gcgctcccat ggtctccccc
     2881 gaaggcccct gcgccgccta ctacctccac cgccacggct gactctccac acgccacgcc
     2941 gcaggcgcgc gtggcgctgc ttgggcccgc cccagcagat ggccagcgca tattttttgc
     3001 tgttcatttg ctggctattg ccgaaaatgg gggttcattt gaaagaaggc acacctatgt
     3061 tccaaggcgc atataccgca ttgatcaccc ccttccaagc tgacgggaag gtggattttg
     3121 gcaagctccg cgagctggtt gagctgcagg tggcgggggg cattgatggc ttggtgccgg
     3181 tgggcaccac gggcgagtcc cccacgctgg acattgatga gcatttggag gtcatccgcg
     3241 tggtgacgga agcggccgcc ggccgggtga agatcatcgc cggcaccggc gccaactcca
     3301 ccgatgaggc gctggaactg acgcgccacg ccaaggattt tggcgtggac ggcacgctgc
     3361 aagtgacccc gtactacaat aagccgacgc aagccggatt ggtccgccat ttttcagcag
     3421 tagccgatct cggcctgccg gtggtgttgt acaacgtgcc cggccgctgc tcatgcgaga
     3481 ttgccgtggg taccattgcc gagctggcca aacaccccca tgtggtttgc gtgaaagagg
     3541 cgggcggcag cgtggaccgc gtgagcaaaa tccgcgcggc atgcgacatc gagattctat
     3601 cgggtgatga cgcgctgacc ctgcccatga tggccgtcgg cgcaagcggg gtcattagcg
     3661 tggcgtccaa cgtggcgcca cgcgcagtgg ccgacttggt ccacgcggcg gcggccggca
     3721 actgggacga agctcgccgg ctgcactacc agtactacgc gctgttcacc gatttgttca
     3781 tcgagaccaa ccccatcccc gtgaaggccg cggcggagat gatggggctg gcgccggcgt
     3841 tgtatcgcct gccgttatgc gcaatggctc cggccaaccg cgacaaactg cgcgcgactc
     3901 tccaacaatg cggcttggtg cgcgcatgag cacgcccatc aagattctgt tggtgggcgc
     3961 ggcagggcgc atggggcaga gcatcacgcg gctgctggct gcgggcacgg tgcccggctt
     4021 ggaactggtg gccgcggtgg accgtgcgga ctcgccattg gccggccaag atgccggcac
     4081 ggcggccggc acgcgcgcgc tgggggtgaa gatcaccagc gatttggctg cggcccttgc
     4141 ggcaaaaccg gcggtggcca ttgatttttc ggcgcacacg ctgacggcgg ctgccgcgcc
     4201 cttgctggcg gcggcgggcg tggcgtgggt gaacggcacc accggactcg aagccgccga
     4261 caacgccgcg gtggcgcagg ccgcccagcg gatccccgtg gtacgctcgg ccaacatgag
     4321 cctcggggtc aacctgctgt acgccttggt ggaacaagcc gcccgcgcct tgcgcggcaa
     4381 ggattacgat tgcgaaatca tcgagcgcca tcaccggcgc aagaaagatg cgccgtccgg
     4441 caccgcgctc tatttgggcg aagcggcggc ccaaggctac gactggccgc tgcgcgacgt
     4501 ggccgtggac ggccgctcgg gtctgaccgg cgcgcgcccc gccaagcaaa tcgggttcca
     4561 cgcggtgcgt gggggcgata ttgtcggcga ccacaccgtc ctgtttgccg ccgatggcga
     4621 atgcgttgag ctggcgcacc gcgcgacctc gcgcgacacc tttgcgctcg gcgcgctgcg
     4681 cgccgcctcg tggcttgccg gccgcgcgcc ggggctctat tccatgcgcg atgttcttgg
     4741 cctcaacccc ggttcgtcat gaatggcatt gaagttggtc tgagcctcgg cgcgaatctc
     4801 gacgacaagc tggccaactt gcgcgccgcc cgcacggcca ttgcggctat tccgtatgtg
     4861 tcggtgctgg cctccgcgcc gctctacgaa accgagcccg tgggcgtacc cgaagaattt
     4921 gccaatctca agtttttcaa cacggtggtg atcatcggca cctcgctcga tgcgcaccgg
     4981 ttgtttgccg aattgcagaa ggtggagctg gccctcggcc gcaagcgcac cctgcgcccc
     5041 aatacgccgc gcgtgattga tattgatatg atttactaca acggccagac catccgcagc
     5101 ggcgggctgg tgatccccca cccccgctgg accaagcgcc gttttgtgct gcaaccgctg
     5161 gccgatgtgc gcggctatct tgtgctgccc ggccacgacc gccgcgtgcg cgacattctc
     5221 gccgcgctgc cgcccgggca ggatatcaaa aagatcatgg tggagtggtg agcatgcgcc
     5281 tgaccccccg ttcgatcacc gcgatgaagg gccgcgagaa aattgcggcg ctcacggcct
     5341 atgactaccg cacggcgcaa ctgctcgatg ccgccggcat cccgctgctg ctggtgggcg
     5401 attcgctcgg caccacgctg ctgggctacg agaacaccct gccggtgacg ctgctcgata
     5461 tgctccgcca cactgccgcc gtggtgcgcg gcgtgaaaaa cgcggtggtg gtggtggaca
     5521 tgccgttcat gacctaccag gcctccatcg agcaggcgct gcgcaactgc ggccgcgcca
     5581 ttcagaaaac aggctgccac gccgtcaaga ttgaaggcgg cgagttccgc gcccccaccg
     5641 tcgccgcgct cgtccaaaat gggattccgg tgctggggca catcggcctc accccgcaga
     5701 gcgtgcaaac gctgggcctg cgcgtgcagg gccgcggcac agaagccgcg cggcagctgg
     5761 tggccgatgc ccaggccatc gccgccgccg gcgctttcgc cattgtgctg gaggccgtgc
     5821 cgagcgcgct cggcgcggag gtcaccgccg ccgtaccggt gccggtgatc ggcattggcg
     5881 ccggccccgg atgcgacggg cagattctgg tgatcaacga tttgctcggt ctctccggcg
     5941 atttcaaacc caagtttgtg aaacaatatg ccaaccttgg cgcgcaaatc agcgcggcgg
     6001 ccagcgccta caaatccgag gtggccggcg ccgtgttccc cggcccggag catggctacg
     6061 attaaccgcg gctgcggccg cgcctgctga tgccatgaag gtcatcaccc actccccgga
     6121 aatgcaacag accgcgctcg ccttgcgcgc ggcgggccgg cggctggcgc tcgtgcccac
     6181 catgggcaac ctgcacgcgg gacacctcgc cctggtgcgg ctggccaaac agcacgcgga
     6241 tgtggtgatt gtctccattt ttgtgaatcc cacccagttc ggccccaacg aggattttca
     6301 aacgtacccg cgcacgttcg aggccgaccg cgcccaatgc gccgcagccg gggtggatat
     6361 tattttttat ccggcggacg tcagcgaaat ctatcccgcc gatgccagca tcacggtggt
     6421 ggaatcggcg ctttcgcaaa ccttgtgcgg ggcatcgcgt ccgggacatt tcactggcgt
     6481 ttgcaccgtg gtggccaagc tgttcaacct gacgctgccg catgtggcag tctttggcga
     6541 aaaagatggc cagcaactgc gcatcatgcg ccgcatggcc cgcgacttgc acttcccggt
     6601 ggaaatcatc gcgggcccca cggtgcggga gcaggacggc ctcgcgctgt cctcacgtaa
     6661 ccagtatctc acccccgccc agcgcgcgca ggccacctgc ctgcgccgcg cattgaccga
     6721 agccgagcag cgttttgccg ccggtgaacg caatccgcaa tgcctcatta cggccatgtg
     6781 gaatctgatt gccgccgagc ccgacacccg gattgactac attgccattg tggatgatga
     6841 aaccctcgca ccggtgttgg agcccatcac ccgcccggcg ctggtggcgc tggcggtttg
     6901 ggtgggccag ccgcggctga ttgacaacac cgttctgcga ccttgatact tggaaggtcg
     6961 gcgaccattc cagcccgaca atccgacccg gctgcttttc ctgagttcga cacctctgaa
     7021 atggaaagtc ataactcctt gggacacagg
//
LOCUS       DAOPNP010000117         7048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_11813_length_7048_cov_8.659660, whole genome shotgun sequence.
ACCESSION   DAOPNP010000117 DAOPNP010000000
VERSION     DAOPNP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11813_length_7048_cov_8.659660"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PKI64_09360"
     CDS             <1..1468
                     /locus_tag="PKI64_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01244.1"
                     /translation="VRGDTALAAVAGVTANGNEREGISLHVQGLDDATATIVDSHANH
                     NLGHGFDLAAVSADGDAYAGVLSSEAIFDMLASMDLLPPNPTYVPAGPCTASDNGRLG
                     LNLLSSNNTGEASAMVDGITVERNGTGGIRMNLISSAGNVFAELADARMRNNVGIGAR
                     LDLNANQNAIAVISDTLSTGNQDRGYQLKMNSDEGEAHALLGGVTASHNTRAGIWAEL
                     SATDVDGVAVVAMTAVNSISNTARGAHFDITGGDSAHLLIGSDALASYEDHFSSFNFL
                     DWFGDMIPQGPSVFSDNGAGGLQATLTGGEFAWLDLRDVTANNNANNGMNMTLTASSI
                     SAEVSNVTARSNSHGLRLEFAGTSANPGERLITTIQRSQFSDNSLNGITVESGYNGPH
                     DYRVRHNVSANNTGNGLRLNFTGAGPYNIDCGGSPLFHGVGQNSFYGNGNRDLRVNNG
                     INVSAQNNWWGQVPPVAGQFAGDGVDWSNWLLTDPNAP"
     gene            complement(1620..2507)
                     /locus_tag="PKI64_09365"
     CDS             complement(1620..2507)
                     /locus_tag="PKI64_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE01245.1"
                     /translation="MSAETPPLNLAAIVQLRMKIFLRSPLRRKLLERIMRLLDKVNGQ
                     QVLEMSGDDAMGVAMQNAGGGGGAQWSCVAVSETQAALLAEAGVEPIHLAAFPELPFE
                     DSTFDALMVSDVLEYVEKPADFMAEVHRIMRSKTRLILHVRRKRKTLVGLFRRLAGLQ
                     DPSRPMKHAGFTPTELFDALKDGFDVQETTGCGRFFTEFVHLLAELFAGVIPASCEVA
                     ALNERRLKRAAAVFTAFTPLFWLATFLDALFFFLPDHHIVLRAKRRMLWVPRVTPRLR
                     DGRSIAEAALGSKIGTAVE"
     gene            2760..3746
                     /locus_tag="PKI64_09370"
     CDS             2760..3746
                     /locus_tag="PKI64_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01246.1"
                     /translation="MDQFLTGGRDPRMYGITVYPDAAGPRFEFANRPHPGQSVRRDFV
                     TPRGLPFPVIQAQSAITDPFPMLLDTSARQTWAVMSALKGLEYRLVAPPTGEYADHVR
                     TEIPGYAGVGNKLIMGKLHMEYPIFYVAPAAGMLGALNRIAPGEPTSAKPTAREKLAR
                     KISVVMGAAAMRNFAFVRFDFPARAVSFSVTRVYQPSAPNVVAGLPLRDWRGRPAVAA
                     TLDGQPLTLVLDTAGDFDLALPGEHAAAGTLRFGDWAVDVEPRTHHELGLPERFPARL
                     GLGVLSQLAVTLDFKNNRIWFEDPNRDLVADAAPDAAESANPQPIHYRGVRP"
     gene            3743..5494
                     /locus_tag="PKI64_09375"
     CDS             3743..5494
                     /locus_tag="PKI64_09375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE01247.1"
                     /translation="MTTAPAKAQWKDAHMHPPGFRARRGLNWFTVGLLYATFYMCRYN
                     FRFAGPGMREEFGFEAQQIADLAGWFSLAYGTGQLVNGLICDRIGGRASMLLGAIGTI
                     LLNIAIGFSPFISNFSAFAVLWMLNGYLQAAGTPGMVKINAAWFNRAERGVFAGIFGF
                     MIQSGQTMISLLAPVILNGFTFLAFAVGPGNWRMLFCIPPIFVGLVAIWFYFISAPSP
                     DEAGYAGTIHDDVDNSAGTTVRVWESFKFVFSNPLVWFYAVAYGCTGAVRHASDQLAP
                     LYFEDVLGFNMKTNLPEIAVATLALIPMVAVAGSVLSGWVSDRFFRGHRYPVAMVLYF
                     TEAVVIATAAIVMGLKLIGPTPAGILLGCLFLVLIAVTANSTHSIVGAAAPMDIGGKK
                     MAGFASGVIDSFQYYGAAIGLFLTGRLLKVSDGNYTFWFGLMTCFAFFGGCAMLSLLL
                     RQKGRQAASRSVIVFMILLVLGGLVFGMPKYRAQKEVHRARVAEEAAALAAAQAQAAE
                     AMALADAQAAEAKPKVRKVKRAPEVADDHRWIAPAMDDDTFQKKATPKPKKKKPKKAA
                     INAPAAPAADAPTPANP"
     gene            5507..6004
                     /locus_tag="PKI64_09380"
     CDS             5507..6004
                     /locus_tag="PKI64_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01248.1"
                     /translation="MSAQHPVTVTPFQLKLKQLCIAVETLFECRRGRVAFLLNMIGTA
                     ALYWAIMVVQARWLAARPWPLLAISHLGQGLLIFWAIIQVVRRLHDLGRTGKLFWAIV
                     IPPWVAWRVADLFHLTERPSLWELWIVLGLMCLWTVWLILQLIFRPGSEGPNGYDGRD
                     FYKKN"
     gene            6084..6860
                     /locus_tag="PKI64_09385"
     CDS             6084..6860
                     /locus_tag="PKI64_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOE01249.1"
                     /translation="MIGAITGDIVGSVYEWANIKHKGFPFFSPRCFFTDDSVLTIALA
                     DTILTGTPYVQNLKTFYAQYPGRGYGGGFDRWARSRDSAPYNSWGNGAAMRISPVGFA
                     YDNLATVLAKAEEYTAVTHNHPEGIKGGQSVAAAIYLARTGQSKTDIKQYVETQFGYD
                     LSRHVDEIRPTYQFNESSQETVPQAIRAFLDSTDFEDAIRTAISLGGDSDTLACITGG
                     IAQAFYGGVPDDIQQKVYSILDDKLGQITREFTKIFCTPR"
BASE COUNT         1369 a         2200 c         2021 g         1458 t
ORIGIN      
        1 agtgcgtggc gataccgcgc tagccgccgt ggctggggtc acggcgaatg gcaacgaaag
       61 agaaggaatc agcttgcatg tgcagggtct ggacgacgcg acggcgacca tcgtggattc
      121 gcacgccaac cacaaccttg gacacggctt cgatctcgcc gccgtttccg ctgatgggga
      181 cgcctacgct ggcgtgctct ccagcgaagc catattcgac atgctggcca gcatggactt
      241 actccccccc aatccaacct atgtgcccgc cggcccttgc actgcgtcgg acaatgggcg
      301 cttggggctg aaccttttga gctcgaacaa cactggcgag gcctcggcca tggttgatgg
      361 cattactgtg gagcgcaacg gaactggcgg catccgcatg aacctcatca gcagcgcggg
      421 caatgtgttt gccgagcttg cggatgcccg catgcgcaac aatgtcggca ttggcgcccg
      481 actagacctg aacgccaacc aaaacgccat cgcggtcatc tcggataccc tgtctacggg
      541 gaaccaagac cgaggctacc aattgaaaat gaattcggac gaaggcgagg cccacgccct
      601 gcttggcggt gtcaccgcca gccataatac gcgtgcgggg atttgggccg agctttctgc
      661 aacggacgtc gatggggtcg ccgtggtcgc catgaccgct gtcaacagta tcagcaacac
      721 cgcgcgtggc gcccattttg atattaccgg cggcgacagc gctcacctgt tgatcggaag
      781 cgatgccctg gcaagctatg aggaccactt cagctcattt aacttcttgg attggtttgg
      841 cgacatgatc ccgcaaggcc ccagcgtgtt ctccgacaat ggcgcgggcg gcctacaggc
      901 aactttgacc ggaggagaat tcgcctggct cgacctgcgg gatgtcaccg ccaacaataa
      961 tgcgaataac ggaatgaaca tgaccttgac tgcctccagc atttctgccg aggtcagcaa
     1021 cgtcaccgct cgcagtaatt cacatgggct cagactggaa ttcgctggta cctctgcgaa
     1081 cccgggcgaa cgcctgatca ccaccatcca gcgcagccag ttcagcgaca acagcctgaa
     1141 tggtatcact gtggagtccg gctataacgg cccgcacgac taccgcgttc gacacaacgt
     1201 gtccgccaac aatactggca acgggctgcg cttgaacttt actggcgccg gcccgtacaa
     1261 cattgactgc ggtggcagcc ccttattcca cggggtcggt caaaattcat tctacggcaa
     1321 cggaaaccgc gatcttcgcg tcaacaacgg aattaatgtc agcgcgcaaa acaactggtg
     1381 gggtcaggta ccgcccgtcg ctggacaatt cgccggcgat ggtgtggatt ggagcaactg
     1441 gctcctcacc gatcccaacg caccctgatc aacacgcgat aaattcccac acgcgatcgg
     1501 cccagccaga tgctggaccg gttgtgtggg gtaacggtct tgagttccgc ggaccacaga
     1561 agggcttgaa tggggcagac cttctagcta tcagcggggc gctgggtggc gcaggttttt
     1621 tattcgacgg cggtgccgat tttggagcca agggcggctt cggcaatgct gcggccgtcg
     1681 cgcaagcgcg gggtcacccg cggcacccac agcatccggc gcttggcgcg cagcacgata
     1741 tggtgatcag gcaaaaagaa aaagagcgca tcgaggaatg tcgccagcca gaacagcggg
     1801 gtaaacgcgg taaagacggc ggccgcgcgc ttcagccggc gctcattcaa ggccgccact
     1861 tcgcaactgg ccgggattac tcccgcgaac agctccgcca gcaggtgaac aaactcggta
     1921 aaaaatcggc cgcacccagt ggtttcttga acgtcaaagc cgtctttgag cgcgtcaaac
     1981 aactcggtgg gagtgaagcc ggcatgtttc atgggacggg atggatcttg caagcccgcc
     2041 agccggcgga acaaacccac cagcgttttg cgtttgcgcc gcacgtgcag aatgagccgc
     2101 gttttggagc gcatgatccg gtggacttcc gccataaaat ccgccggctt ttcgacatat
     2161 tccagcacat cgctcaccat cagggcatcg aatgtgctgt cttcaaacgg caattcggga
     2221 aatgctgcca aatggatcgg ttccacccca gcctcggcca gtaaggcggc ctgggtttcc
     2281 gataccgcca cgcaactcca ttgggcgcca cccccaccgc ccgcgttttg catggccaca
     2341 cccatggcat cgtcgccgga catttccagt acctgctggc cattcacttt gtccagcagg
     2401 cgcatgatcc gttccaggag tttgcgccgc agcggcgaac gcaaaaagat tttcattcgc
     2461 aactggacga ttgcggccag attcaatgga ggagtttctg cgctcattac gggccaaact
     2521 atgccacccc cgcccgcctc ctgtccagtc cagaccccaa cggacgcgga gctttttccg
     2581 cttcaaaagc gggcggaggt tccgtccggt cttgcatttc accggctacg tctgcatagt
     2641 cccattcctg catgaggcct gtcctgcgca tttgttttct tctggccacg gcgacccttt
     2701 ggggcgtcgg ctgcgcttcc ccgcccccgt tgcgccaaac gccgctcacg caagaccagg
     2761 tggatcaatt tttaaccggc gggcgtgacc cgcggatgta cggcatcacg gtctatccgg
     2821 atgccgccgg tccgcgcttt gagtttgcca accgccccca ccccggccag agtgtgcgcc
     2881 gcgattttgt cacgccccgc ggccttccct ttcctgtcat tcaggcccaa agcgccatca
     2941 cggatccttt cccgatgcta ctcgacacct ccgcacgcca gacgtgggcc gtgatgagcg
     3001 cactgaaggg gttggaatac cggctggtgg ccccgcccac cggtgaatat gccgatcatg
     3061 tgcgaacgga gatccccggt tatgccggcg tgggcaataa actcatcatg ggcaaactcc
     3121 acatggaata ccccattttt tatgtggcgc cggccgccgg catgctcggt gcgttgaacc
     3181 ggattgcccc gggggagccc accagtgcca agccaactgc gcgcgaaaaa ttggcgcgca
     3241 agatttcggt ggtgatgggc gcggcggcca tgcgtaattt tgcgtttgtg cgttttgact
     3301 ttccggcgcg cgcggtttct ttttcggtca cgcgcgttta tcaaccgtcc gccccaaacg
     3361 tggtggccgg gttgccgctg cgcgattggc gcggtcgtcc ggccgttgcg gccacgctgg
     3421 atggccagcc tcttacgctg gtgttggaca cggccggtga tttcgacctt gccctccccg
     3481 gtgaacacgc cgccgccggc acgctgcgct ttggcgattg ggcggttgac gttgaaccgc
     3541 gtacccatca cgaattggga ctgcccgaac gcttccccgc tcgtctcggc cttggcgtat
     3601 tgagccaact tgccgttact ttggatttta aaaacaaccg gatctggttt gaagatccaa
     3661 accgggatct ggtcgccgat gctgcgccgg atgctgctga atccgccaac ccacaaccga
     3721 tccactaccg aggagtcagg ccatgaccac tgccccagcc aaagcccaat ggaaagacgc
     3781 acacatgcac ccgcccggat ttcgggcacg gcgcggcctc aactggttca ccgtgggcct
     3841 gttgtatgcc acgttttaca tgtgccgtta caacttccgc tttgccggcc ccggcatgcg
     3901 cgaagagttc ggtttcgagg cccagcaaat cgcggatttg gccggctggt tttcgctggc
     3961 ctacggcacc ggccagttgg tcaacggcct gatttgcgac cggattggag gccgcgcttc
     4021 gatgttgctg ggcgccatcg gcaccattct gttgaacatt gccattggtt tttcgccgtt
     4081 catctccaac ttctccgcat tcgcggtgtt gtggatgctc aacggctatc tgcaagccgc
     4141 cggcactccg ggaatggtta aaatcaacgc cgcctggttc aaccgcgccg agcgtggcgt
     4201 gtttgcgggg atcttcggct tcatgatcca gtccggacaa accatgatca gcctgctcgc
     4261 gccggtcatc ctgaacggct tcacgttcct ggcctttgcg gtgggcccgg gtaactggcg
     4321 catgttgttc tgcatcccgc cgattttcgt cggactggtg gccatctggt tctatttcat
     4381 ttctgcgcca tcgcccgacg aagccggcta tgcgggcacc atccatgacg atgtggacaa
     4441 ttccgccggc accaccgtgc gcgtgtggga gtcgttcaag tttgtctttt ccaacccgct
     4501 ggtctggttt tatgccgtgg cctacggttg cacgggcgcc gtgcgccatg cttctgacca
     4561 gcttgccccg ctgtactttg aagacgtgct gggcttcaac atgaaaacca acctgccgga
     4621 aatcgcggtg gccacgctcg cgttgattcc catggtggct gttgccggct cggtgctgtc
     4681 gggctgggta tcggaccgct ttttccgcgg tcaccgctac cccgtcgcca tggtgcttta
     4741 tttcaccgag gccgtggtga ttgccaccgc ggccatcgtc atgggcctga agctgattgg
     4801 ccccaccccc gccgggattc tgctgggctg cctgttcctg gtcttgattg cggtaaccgc
     4861 caacagcacg cactccatcg tgggcgccgc cgcgccaatg gatattggcg gcaagaaaat
     4921 ggccggcttt gcatctggtg taattgattc cttccaatat tacggcgcgg ccattggcct
     4981 cttcctgacg ggccgtctgc tcaaggtatc cgacggcaac tacacctttt ggttcggact
     5041 gatgacctgc ttcgccttct ttggcggctg cgcgatgctc tcgttgctgc tgcgccaaaa
     5101 gggccggcaa gccgccagcc gcagcgtaat tgtgttcatg atcctgctgg tgctgggcgg
     5161 gttggtgttt ggaatgccca aatatcgcgc acaaaaagaa gtacaccggg cgcgcgtggc
     5221 cgaagaagcc gccgcgctgg cggcggcaca agcccaagcc gccgaagcca tggcgctggc
     5281 cgacgcccaa gcggcggagg ccaaacccaa ggtccgcaag gtgaagcgcg ccccggaagt
     5341 ggccgacgat caccgctgga tcgcaccggc catggatgat gacaccttcc aaaagaaggc
     5401 cacgcccaag cccaagaaga aaaagcccaa aaaggctgcc atcaatgcgc cggcagcccc
     5461 cgcggccgat gcccccaccc ccgccaaccc ctaacggagc aatcccatga gtgcacaaca
     5521 ccccgtgact gtcacgccct tccaactcaa gctcaagcaa ctctgcattg cggtggaaac
     5581 tctctttgag tgccgccgcg gccgcgtggc ctttctactg aacatgatcg gcacagcggc
     5641 cttgtactgg gccatcatgg ttgtgcaggc gcgctggctg gcggcgcggc catggccgct
     5701 attggccatc tcccatctcg gccaagggct cctgattttc tgggccatca ttcaggtggt
     5761 ccgccgcctg cacgacttgg gccgcactgg caagctgttt tgggccattg tgattccgcc
     5821 atgggttgcc tggcgcgtag ccgatttgtt ccatttgacg gaacgaccct cgctgtggga
     5881 gctctggatc gtgctgggcc tgatgtgcct ctggactgtg tggcttatct tgcaactcat
     5941 cttccggccc ggctcggaag gccccaacgg ctatgacggc cgcgacttct ataagaaaaa
     6001 ctaggctgaa taagctggtt cccccttgcg gcatgtgcgg gccgcactga acccccgacc
     6061 ggcggaggac ccgcggaggt aaaatgatcg gcgcgattac gggagatatt gtcggctccg
     6121 tctacgaatg ggccaacatc aagcacaagg ggtttccttt cttttcgccg cgctgttttt
     6181 tcacggatga ctccgtgctg accatcgcgc tggcggacac catcttgacc ggcactccat
     6241 acgtccaaaa tctcaaaaca ttttatgcgc agtatcccgg tcggggatac ggcggtggtt
     6301 ttgaccgttg ggcgcgcagc cgagactccg cgccatacaa tagttggggc aatggcgcgg
     6361 ccatgcgcat cagccctgtg gggttcgcgt atgacaactt ggccaccgtg ctggccaagg
     6421 ccgaagagta taccgccgtc actcacaacc acccggaagg catcaagggc ggtcaatccg
     6481 tcgccgccgc gatctacttg gcgcgcaccg gccaaagcaa aaccgacatc aagcaatacg
     6541 tcgaaacgca atttggctac gacttgagcc gccatgtgga tgaaatccgc ccgacatacc
     6601 aattcaacga atcttcacaa gaaaccgtgc cacaagccat ccgtgcgttt ttggattcaa
     6661 ccgattttga agacgccatc cgcaccgcca tatcactggg gggtgattct gacaccttgg
     6721 cttgcatcac tggcgggatc gcgcaggctt tttatggcgg cgtaccggat gatatccaac
     6781 agaaggtcta ttccattctc gacgacaaac tcgggcagat cacccgagaa ttcacgaaaa
     6841 ttttttgcac tccgcgctga aatcgcgtcg tcatacgcga cgggtcccac ataagatcgg
     6901 cgatcattcc ataccctata atccaacccg gatgtttttg tagggcgaga acggttgagg
     6961 gacgaaacct tcccgccgag aacggctgga agatctgcga ccgttccagc ccttctatgg
     7021 gggccgtcaa gtcatggcga tggattta
//
LOCUS       DAOPNP010000118         6895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_12133_length_6895_cov_7.733041, whole genome shotgun sequence.
ACCESSION   DAOPNP010000118 DAOPNP010000000
VERSION     DAOPNP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12133_length_6895_cov_7.733041"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKI64_09390"
     CDS             complement(<1..199)
                     /locus_tag="PKI64_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01250.1"
                     /translation="MTDFEKMGVFYLGRVFDPAMQQTTAEPLLYDAKDLTTHAVCLGM
                     TGSGKTGLGIGLLEEAAIDGIP"
     gene            complement(274..2499)
                     /gene="priA"
                     /locus_tag="PKI64_09395"
     CDS             complement(274..2499)
                     /gene="priA"
                     /locus_tag="PKI64_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOE01251.1"
                     /translation="MPQPIARIITDIALDREFDYRIPEQLLGRVQVGSVVQIPFGRRH
                     ARGFVTGLRDTSPFPDLKALHDVVDAPPLFDESMLRLARWLADYYVAPFEQAIAAILP
                     APVRREGAKFKEQLIVRARSESAPTPEEVAALAKRSPKQAAAWQELLQTPEQTAAALM
                     AASGVGLPGLRALAAKGWLTLEKGVIRRNPHERMQVLPTHPPPLMAEQTAALAAIHEE
                     MTAAKPRVVLLHGVTGSGKTEVYLAAIQAALDAGKGAIALVPEIALTPQTMERFRGRF
                     GETVAVLHSHLSDGERHDEWRRIAAGQARVVVGARSALFAPVRPLGLVVVDEEHEPAY
                     KQDEAPRYNARDVAVMRGHLEGCAVVLGSATPSLESYRHARAGKYRLVNMTQRVDEQV
                     MPMVRVVDMRLETEKAGGAHLISQDLIEAVRQRLDQGEQTILFLNRRGYATTVMCPLC
                     GHTETCPDCSVKMTYHKEAEFLLCHVCGHTSPVPARCSAPQCGAPNIKMAGVGTQRVE
                     AAVRKIFPRARVARMDSDATGAKDSHAQILGKFRRGDIDILIGTQMIAKGLDFPNVTL
                     VGVIHADLSLQMPDFRAAERTFQLLTQVAGRAGRGDVPGEVLIQTYTPFHPAVQASRR
                     ADYTTFYDQEIELRRELNYPPFSRLTTVLFRGLEEANVNTAAKDFAERLQKLLPAGVR
                     LLGPNPAPLARAKKQYRTHFLLFGRNTRDYIPALREILTSFKPPKGVAIAINVDALSI
                     M"
     gene            complement(2715..4007)
                     /locus_tag="PKI64_09400"
     CDS             complement(2715..4007)
                     /locus_tag="PKI64_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOE01252.1"
                     /translation="MSQGLHTISLHAGQKADPTTGSRAVPIYQTTSYVFKSTEHAANL
                     FALKEFGNIYTRLMNPTTDVFEQRVAAIEGGTGALAVASGQTAITYALLAITRLGDEI
                     VAGNNLYGGTYQLLHHTFPKLGRTTKFVNSRDPEAFRRAITEKTRAIYVETIGNPKLD
                     VPDFEALAKIAHENGIPLVVDNTIGVGLVRPFDHGADIIATSATKYIGGHGTSVGGVI
                     VDSGKFRWDNGKFPEFTEPDPSYHGLKYWDALGNVPGMGNVAFILKIRVTLLRDTGAA
                     LSPFNAHEFLLGLETLPLRQQRHSENALAVARWLKQHSAVSWVAYPGLADDPGHAVAA
                     KYLKKGFGGMVGFGIKGGLEAGKKFINSVQMLSHLANIGDSKTLVIHPASTTHQQLTA
                     EEQQATGVTADYIRLSVGLEDVADIQADIDQALKKAVS"
     gene            4242..5201
                     /gene="cysK"
                     /locus_tag="PKI64_09405"
     CDS             4242..5201
                     /gene="cysK"
                     /locus_tag="PKI64_09405"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOE01253.1"
                     /translation="MSRIYRDNSESIGNTPLVRLNKVVAGAPATVLAKVEGRNPSYSV
                     KCRIGAAMIWDAEQRGVLKPGVEIVEPTSGNTGIALAYVAAARGYKLTLTMPETMSLE
                     RRRVLAALGANLILTPGAEGMKGAIARAEAMAAAEPQKYFLPQQFRNPANPDIHERTT
                     AVEIWNDTDGNMDVLVSGVGTGGTITGVSRYFKKIKGKALVSVAVEPKESPVITQRLA
                     GQELKPGPHKIQGLGAGFIPDTLDLSLVDRVEQVDAAESVEMARRLAKEEGILSGISS
                     GAAVVAAARLAHDPAFAGKTIVVILPDAGERYLSTILFEGIGA"
     gene            complement(5214..>6895)
                     /locus_tag="PKI64_09410"
     CDS             complement(5214..>6895)
                     /locus_tag="PKI64_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOE01254.1"
                     /translation="RQVQVKIPSLCYHPDLPPWAACGICVVKVKGLNKLVRACAAPAA
                     EGQEIITHDSELFAIRRTIIELILSTHPDDCLQCPRNQNCELQRLAAEFGIREAPYGK
                     RLRDLPEDDTTGSLVLNPEKCILCGRCAAVCQDMQGVWALEFIGRGENTRIAPAADVT
                     LNESPCIKCGQCSAHCPVGAIYEHSHAREVYAALQNPAKHCVVQIAPAVRVALGEDFG
                     YPPGTLLTGKIYAALRRLGFDAVFDTNFGADLTIMEEGTEFVERFVHGKGELPLITSC
                     CPSWTDYMEKFAPDFIHNFSTAKSPHAMVGALTKTYYAEKMGLDPKDIFMVSIMPCTA
                     KKFEIQRSAEMSSSGQQDIDISLTTRELCRMIKSAGIDFRNLPEDERVDSLLGEYSGA
                     GTIFGVTGGVMEAALRTAHWALTKERLPSMELKEIRGLDGVKEATLQVAGKEVRVAVA
                     HCMGNIETVLDRVRAARAAGKETPYHFIEVMACRGGCVGGGGQPYGATDKIRRQRAAG
                     IYRDDEKSAQRCSHENPMIQKLYADYLGAPLSKKSHQLLHTHYQQRKVYKR"
BASE COUNT         1314 a         2128 c         2027 g         1426 t
ORIGIN      
        1 ccgggatgcc atcaatggcg gcttcttcca gcaagccgat gcccagcccg gttttgccgg
       61 agccggtcat gccgaggcaa accgcgtgcg tggtcaggtc tttggcatcg tatagcagcg
      121 gttcagccgt ggtttgctgc atggcgggat caaacacccg gcccaaataa aacacgccca
      181 ttttttcaaa gtccgtcatg tggctgctcc ttgttccatc ggcgccagtg tacaacccgg
      241 cggctcgctt gcccagtccg gggagggatt ttgctacata atggacagtg cgtccacatt
      301 gatggcaatg gccactccct tgggcggttt gaaagaggtc aaaatttcac gcagggctgg
      361 gatatagtcg cgggtgttgc gcccgaacaa caggaagtgc gtacggtact gctttttggc
      421 gcgcgccagc ggcgctggat tgggccccag cagccggaca cccgccggta atagtttttg
      481 gagccgctcg gcgaagtctt tggcggcggt attcacgtta gcctcctcca gaccgcggaa
      541 caataccgtg gtcaggcggc tgaagggtgg atagttcaac tcgcggcgca gttcaatttc
      601 ctgatcgtaa aaagtggtgt aatcggcccg gcgcgacgcc tgcaccgcgg ggtggaacgg
      661 ggtgtaggtc tggatcagca cttcgccggg cacatccccc cgccctgccc gtcccgccac
      721 ctgcgtcaag agttggaagg tgcgctccgc ggcccggaaa tccggcatct gcaaacttaa
      781 gtcggcgtga atcacgccca ccagcgtgac gtttggaaag tccaagcctt tggcgatcat
      841 ctgtgtgcca atcagaatat caatgtcgcc gcgccggaat ttgcccaaaa tttgcgcgtg
      901 ggaatctttg gcaccggttg catcggagtc catccgcgcc acccgcgccc gcgggaatat
      961 cttgcggacg gcggcctcga cgcgttgtgt gccgacgccc gccatcttga tgttgggcgc
     1021 gccgcattgg ggcgcgctgc agcgcgcggg caccgggctg gtgtggccgc acacatggca
     1081 cagcaggaac tcggcctctt tgtggtacgt cattttgacg ctgcaatcgg ggcaggtttc
     1141 cgtatgcccg cacagcgggc acatgaccgt ggtggcatag ccgcgccgat tcagaaacag
     1201 gatcgtctgt tcgccttgat cgaggcgctg gcggacggct tcgatcaagt cttgcgagat
     1261 caggtgcgca ccgccggcct tttcggtctc caagcgcata tccaccacgc gcaccatggg
     1321 catgacttgt tcatccacgc gctgcgtcat gttgaccagc cgatatttgc ccgcgcgggc
     1381 gtggcggtag gattccagcg acggcgtggc gctgcccagt accaccgcgc agccctccaa
     1441 atggccgcgc atcacagcga catcgcgcgc gttatagcgg ggggcctcgt cctgcttgta
     1501 ggccggttcg tgttcttcat ccaccaccac cagccccagc ggtcgcaccg gcgcgaacaa
     1561 agcgctgcgc gcgccaacta ccacgcgcgc ctgcccggca gcaatccggc gccattcgtc
     1621 gtggcgctcg ccatccgaaa gatggctgtg caggaccgcg accgtttcgc caaagcgccc
     1681 ccgaaaacgc tccatggttt gcggggtcag ggcaatctcc ggcaccagcg cgatggcccc
     1741 cttgccggca tccaaagccg cttggatggc ggccagatag acttctgttt tgccgctgcc
     1801 agtcacgccg tgcaatagca ccacgcgtgg cttggcggcg gtcatttctt catggatggc
     1861 tgccaaggcg gccgtctgtt ccgccatgag cggcggtggg tgcgtgggca gcacctgcat
     1921 gcgttcatgc ggattgcgac ggatcacgcc tttttcgagc gtcagccagc ctttggctgc
     1981 caacgcgcgg agccccggca ggcccacgcc gctggcagcc atcaatgcgg cggcggtttg
     2041 ctcgggcgtc tgcagcaact cttgccatgc cgcggcttgc ttggggcttc gctttgccag
     2101 cgccgcgact tcttccggtg ttggcgcact ctccgaccgc gcgcgcacaa tgagttgttc
     2161 cttgaacttg gcgccctcgc gccgcacggg ggcaggcaaa atggcggcga tggcctgctc
     2221 aaacggcgca acataataat ccgccagcca acgggccagc cggagcatgg attcatcgaa
     2281 gagcggcggg gcatccacca cgtcatgcag ggccttcaag tccggaaacg gcgaagtgtc
     2341 gcgcagaccg gtgacaaacc cccgggcgtg gcgtcggcca aagggaatct gcaccacgct
     2401 gcccacctgc acccgcccca gcagctgctc cggaatgcgg tagtcaaact cgcgatccag
     2461 cgcaatgtcc gtgatgattc tggcaatggg ctgcggcaca acgggacatc ctacatgtcc
     2521 ttggtgaggt aaatcaccat gccgaatatc cacaggatca tggcggccga gatgacagtg
     2581 ttcatggccc ggactatcgc acagtttgcc gcggcggaaa agcttttccc cacccccagc
     2641 ccggctacca aaaaacaacg gcgacttcgc acgaggcggg tcgccgttgg gtcaacgtca
     2701 gacactgacg tcgtttagga cactgctttt ttcagcgctt ggtcaatgtc tgcctgaata
     2761 tcggccacgt cttcaagccc caccgacagc cggatgtagt ctgcggtgac tccggtggcc
     2821 tgctgctctt cggccgtcag ttgctggtga gtggtcgaag ccgggtgaat caccagcgtc
     2881 ttggaatcac cgatgttggc caagtgcgag agcatctgga cggagttgat gaactttttg
     2941 ccggcttcca aaccgccctt gatgccaaag cccaccatgc cgccaaaccc tttcttcaag
     3001 tattttgcgg ccacggcatg gccggggtcg tcggcgaggc ccggataggc cacccaactg
     3061 acggcggaat gctgtttcaa ccagcgggca acggccaacg cgttttccga atggcgctgt
     3121 tgccgcagtg gcaaggtttc caggcccagc aaaaactcgt gggcgttgaa cgggctcagc
     3181 gccgcgccgg tatcgcgcag caacgtcacg cggattttca aaataaacgc cacgttgccc
     3241 atgccgggca cattccctaa tgcgtcccag tatttcaggc cgtggtagct cgggtccggc
     3301 tccgtgaatt ccgggaactt gccattgtcc caacggaact tgcccgaatc cacaatgacc
     3361 ccgcccacgg aggtgccatg cccgccaata tatttggtgg ccgaggtggc gatgatgtcc
     3421 gccccatggt caaacgggcg caccagcccc acgccgatag tgttgtccac caccagcggg
     3481 ataccgttct cgtgggcaat cttcgccagc gcctcaaaat cgggcacatc cagtttcggg
     3541 ttgccaatgg tttccacata aatggcgcgg gtcttctcgg tgatggcacg gcggaacgct
     3601 tccggatcgc gcgagttcac aaacttggtt gtccggccga gtttcgggaa ggtgtggtgc
     3661 agcaactggt aggtgccgcc gtaaagattg ttgccggcca caatctcatc gccaagccgg
     3721 gtaatggcca acaacgcgta cgtaatagcc gtttgcccag aggccacagc caacgcgcct
     3781 gtgccgcctt cgatggctgc cacccgctgt tcgaacacat ccgttgttgg gttcatcagc
     3841 cgcgtgtaga tgttgccgaa ttcttttaac gcaaaaaggt tggccgcgtg ttccgtgctt
     3901 ttgaacacgt aggatgtggt ttgatagatc ggcaccgcgc gcgacccagt ggtcgggtcc
     3961 gccttttggc cggcatgtaa cgaaatggtg tgaagtcctt ggctcatgtt gatctcctgc
     4021 tgtttttaga ttctttttca ggacgggagg gcaagtcctt tttgtatgtt ttccaaccca
     4081 gaccgtcgag ttttttggtt aatttttatt tctctacatt atccatcgtc tgactggact
     4141 atctgtcatt ttcacaccac tgtcaagagt tacgcaaaaa atatttttga cattaacatt
     4201 ttatatagta tttctataga cattaaaaac aaaggagccc catgagcaga atctatcgcg
     4261 acaattcaga atccatcggc aacactccgt tggttcgttt gaataaagtt gtggccggtg
     4321 cgccggcaac ggtcttggcc aaagtggaag ggcgcaatcc ctcctactcc gtcaagtgcc
     4381 ggatcggtgc ggcgatgatc tgggatgccg aacaacgcgg cgtgctgaaa cccggcgtgg
     4441 aaatcgttga acccaccagc ggcaacaccg ggatcgccct cgcctatgtg gccgcggcgc
     4501 gcggttacaa actgacgctc accatgcccg aaaccatgag cttggaacgc cgtcgggttt
     4561 tggccgcttt aggtgccaat cttattctga cccccggcgc cgaaggcatg aagggcgcca
     4621 ttgcgcgcgc ggaagccatg gctgccgccg aaccccaaaa atattttctg ccccagcaat
     4681 ttcgcaatcc ggccaacccg gacatccatg aacgcaccac ggcagtggaa atttggaatg
     4741 acacggacgg caacatggat gtgctggttt ccggcgtcgg caccggaggc accatcaccg
     4801 gcgtttcccg ctacttcaaa aaaatcaagg gcaaggcgct ggtttcggtg gctgttgaac
     4861 cgaaggaaag cccggtgatc acgcagcgcc tggccggcca ggagctaaag cccggcccgc
     4921 ataaaatcca agggctcggc gcagggttta ttcccgacac gttggatctg tcgttggtgg
     4981 atcgcgtgga gcaggtggac gcagcggaat cagtggaaat ggcgcgccgc ctggccaagg
     5041 aagaagggat tctctccggc atttccagtg gcgcagcggt cgttgctgcg gcccgtttgg
     5101 cgcacgatcc agcctttgcc ggcaaaacca tcgtggtcat cctccctgac gcaggcgaac
     5161 gctatctctc caccatcctc ttcgaaggca tcggcgccta gcgcccaccg tgttcagcgc
     5221 ttatatactt tgcgctgttg gtaatgggtg tgcaggagct ggtgggattt cttgctcaac
     5281 ggcgcgccga ggtaatcggc gtatagcttc tggatcatcg ggttttcgtg ggaacagcgc
     5341 tgggcgctct tttcatcatc ccgatagatt cccgccgccc gttgccgccg gattttatcc
     5401 gtcgcgccat acggctggcc accgccgccc acgcacccgc cgcgacaggc catcacttca
     5461 atgaaatgat agggcgtttc cttgccggcc gcgcgcgcgg cgcgtacccg gtccaaaacc
     5521 gtctcaatgt tgcccatgca atgggccacg gccacgcgta cctctttgcc ggccacctgc
     5581 aacgtggctt ctttcacccc gtccagcccg cggatttctt tcaattccat ggatggcaga
     5641 cgttctttag tcagcgccca gtgcgcggtg cgcagcgcgg cttccatcac gccgccagtc
     5701 accccaaaga tggtgcccgc gccggaatac tcgcccagca gcgaatccac gcgttcgtct
     5761 tccggcaggt tgcggaaatc aatcccggcg ctcttgatca tccggcacag ctcgcgagtc
     5821 gtcagcgaaa tatcaatgtc ctgctggccg gagctgctca tttcggcgct gcgctgaatc
     5881 tcaaacttct tggcggtgca gggcatgatg gacaccatga agatatcctt cgggtccaag
     5941 cccatttttt ccgcataata ggttttggtc agcgcgccca ccatcgcgtg cggcgatttg
     6001 gccgtggaaa agttgtggat gaagtccggg gcgaacttct ccatgtagtc ggtccacgac
     6061 gggcaacagc tcgtgataag cggcaattct cccttgccat ggacaaagcg ctccacgaat
     6121 tcggtgcctt cctccatgat ggtgaggtcg gcgccaaagt tggtgtcgaa caccgcatca
     6181 aaacccagcc gccgcagcgc ggcatatatc ttgccggtga gcagcgtgcc gggcgggtag
     6241 ccaaagtctt cgcccagcgc cacacgcacg gcgggggcaa tctggaccac gcaatgtttg
     6301 gcggggtttt gcagcgcggc atagacttcg cgggcatggc tatgctcata aatggcgccc
     6361 accgggcaat gcgcggagca ttgcccgcat ttgatgcagg gcgactcgtt caacgtcaca
     6421 tccgccgctg gggcgatgcg ggtattctcg ccgcgcccaa taaactccag cgcccacacc
     6481 ccctgcatat cctggcacac cgccgcgcaa cggccgcaca aaatgcattt ttccgggttt
     6541 agcaccagcg agccggtggt gtcgtcttcg ggcaaatcac gcaaccgctt gccgtagggc
     6601 gcttcgcgga tgccaaattc cgccgccaac cgctgcaatt cgcagttttg gttacgcggg
     6661 cattgcaggc aatcgtccgg atgcgtggac aaaatcagtt caatgatcgt gcggcggatc
     6721 gcgaacaact ccgagtcgtg cgtaatgatc tcctgtccct cggctgccgg cgcggcgcac
     6781 gcgcgaacca gcttgttcag gcccttcacc ttcaccacgc aaatcccgca tgcggcccat
     6841 ggcggcaagt cggggtggta gcacaggctc gggattttca cctgcacctg gcggg
//
LOCUS       DAOPNP010000119         6793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_12354_length_6793_cov_8.282725, whole genome shotgun sequence.
ACCESSION   DAOPNP010000119 DAOPNP010000000
VERSION     DAOPNP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12354_length_6793_cov_8.282725"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKI64_09415"
     CDS             complement(<1..341)
                     /locus_tag="PKI64_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01255.1"
                     /translation="MILATGLMPRQTEAAYEGSGTFKLITNTNDITSGGYYVFVGAPS
                     WASTAMGTGRMTAASTAALSTYAVSYASGNIVNPAAGIVWRIDATSSSWSIYSEAASF
                     YVGQPNANGVRT"
     gene            complement(952..1359)
                     /locus_tag="PKI64_09420"
     CDS             complement(952..1359)
                     /locus_tag="PKI64_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOE01256.1"
                     /translation="MKSAVKAWLCKAEADAISARREFRARKRPNFDSACFHAQQCVEK
                     TIKAILESANCPIRKVHDLVVLLTQCPTGAARTELLRDDLELLSQYAVTFRYPGADAT
                     REQAKLAISAMDRCREQLLPLLSSSRGARGKKK"
     gene            complement(1343..1657)
                     /locus_tag="PKI64_09425"
     CDS             complement(1343..1657)
                     /locus_tag="PKI64_09425"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOE01257.1"
                     /translation="MILRQVQQYVDHLVEQFRPERVILFGSMAEGRATADSDVDLMVV
                     MAGTQNTTDSALEIRRRIPRSFPLDLVVKTPGEVRRGMQGRDGFICSVLERGKVLYEK
                     RR"
     gene            complement(1654..2079)
                     /locus_tag="PKI64_09430"
     CDS             complement(1654..2079)
                     /locus_tag="PKI64_09430"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOE01258.1"
                     /translation="MKVLNENIPPALRHPSTGLAHCLRALGQARPVQRVVLFGPYARG
                     EQRPDSDVDLCIVAEGAEDQLAAARDFRRALRNVRPKPAFTLIPITPARFEEKKAAGD
                     YFFKTIQEEGICVAEKVALGERRRPALSPRRKERQGVAP"
     gene            complement(2458..2823)
                     /locus_tag="PKI64_09435"
     CDS             complement(2458..2823)
                     /locus_tag="PKI64_09435"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOE01259.1"
                     /translation="MNRALQTTNLTDRPESRRTLGSVQLDARLIAPIVAAITAHKPVR
                     RIWLYGSRAAGVARAASDVDLAIEADNWSDSDTNLAHDRLESEVPTALKFDLVNVNAV
                     TKPRFRESIQRHGKVIYEA"
     gene            complement(2984..3475)
                     /locus_tag="PKI64_09440"
     CDS             complement(2984..3475)
                     /locus_tag="PKI64_09440"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOE01260.1"
                     /translation="MTRVASRTVLRQMLAKARRKLAAARRELKARYFEEATTHAYYAA
                     FHAVTAILATQGLAFSSHAQTIGAFNARFVRAGLFDKEFAKILHRLFGNRQLADYDWM
                     FAGDAKSAREDVQDAAALVARCHGFIRQFGVEQKTVAALRSTKSAPSKVISAKKKSKE
                     GRP"
     gene            complement(3472..3825)
                     /locus_tag="PKI64_09445"
     CDS             complement(3472..3825)
                     /locus_tag="PKI64_09445"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOE01261.1"
                     /translation="MKRQRRSRRIPNAACAIPRLARRYAQAIRQDLTDQVRLIALYGS
                     RARGDAHAGSDYDFLVVLSSPTQKERETVTAAGAKLLAETDQLCTALVYSPEQWERVQ
                     QSPLGWNILREGVEL"
     gene            complement(3929..>4298)
                     /locus_tag="PKI64_09450"
     CDS             complement(3929..>4298)
                     /locus_tag="PKI64_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01262.1"
                     /translation="PPRKPELTQASLTSQKPAKEPKLNHSSTRVKTSLPIEGWHDAAV
                     LKATVTSPSAAVRPFAALASFALMFSGLLSPCLVSHRRRHACCQPASGQRVDNRPKGL
                     AQKKRNIRKIWRIMPRKTVY"
     assembly_gap    4299..4398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4591..6744)
                     /locus_tag="PKI64_09455"
     CDS             complement(4591..6744)
                     /locus_tag="PKI64_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01263.1"
                     /translation="MTRWLASERGRMAGACLLVMVVAFALYLPTIRYDFVTLDDPMYV
                     ENSDVVRDGFTREGLQLAFTTAPENYWAPLLWMSFMLDKEISGGPPWSCHLTNTILFA
                     LNAGLLFLLVWQWTGGRAGVALATALLWACHPTRVESVAWVTARKDVLSGLFLLAGLW
                     FYTAAHQAGPSSQNPAARMRHPALFLGLAWLCMVVGGMAKQVVIIMPAAFVLLDVWPL
                     GRTDWDRIWKDGWRLVAEKWAFWLPAVAFASLPIIFHVETKAVMDVPAWHRAMMIPVH
                     YLFYLSKVLWPARLMPLQADLPSWWELLMGATLVLGGFTWGAWRWRKRFPLALWGWLW
                     FAILLFPLSGVVWAGAERVATRWLYIPQIGLLLAAVAALAEVQRVRGWKMRWNVAVAA
                     VVIAAWAGMSLRLSRHWRSRDDFGIWAYTCNPNHAVACMLGGDGYLAKKDWAQAAKAY
                     EQGSSFWETHSFLRLCLIWNCCGFPELTDDAWPRFEKGLGRSLLEALKDQRPLEREFL
                     WRVRGQCMQARGDLAGAIGAFKEALALDEDPNAFVVAEYLRASHEADAFDEEAQAVAR
                     RMTEAAGEPRMTWADLFPCYAQIWRDGARGYAYGYFLEFARREPTNAVAFNNMAWLLA
                     TAKPTKINHHREEEWPAKALEWAEKAANSEEGKQAATAWDTLGAARANAGDFAGAIEA
                     ALRGARLARRDGGKELAAEIEKRLAEYRLGKPWRE"
BASE COUNT         1338 a         2115 c         1864 g         1376 t
ORIGIN      
        1 gtgcgcacac cgttggcgtt gggttgccca acgtagaagc tggctgcctc gctatagatg
       61 gaccaagaag atgatgtagc atcaatcctc cagacgattc ctgcagcggg gttcactatg
      121 ttaccgctgg cataggacac cgcataggtg gatagcgcgg cggttgatgc cgcggtcatc
      181 cgcccggttc ccattgccgt cgatgcccat gaaggcgcac ccacaaaaac ataatacccg
      241 ccagaggtga tatcgttcgt attcgtaata agcttgaagg ttccggagcc ttcatacgcc
      301 gcctcggtct ggcgaggcat caagcccgtc gcgaggatca ttgccgccaa accccactgc
      361 gccagtctgc cccgcgtgcc acgacgcgac tccacacccc tcccgccccg cttgtcgcgg
      421 gtggcctgtc cggactgacc gcgctcaggc gcggcgcacc gcccgcccgt caacagccaa
      481 gccgcagccc tcatccaaaa aggccgcaat gtcccggagg tgtgtcgtgt tctcatggtc
      541 tcactccaaa ggggtagtcc taaattgata aattcttcca tctgctcgat ttgttttgcc
      601 cgcggggtca ccgccccctg tagccggcct cgctgaggcc ggggctgttc ctgtagccgg
      661 cctcgttgag gccggggctg ttccagatcc caaaatccgc gcaacagcgg ggtcaccgcc
      721 cccgcctaca gtccaaccca tgccgccccc tgtagccggc ctcgttgagg ccggggctgt
      781 tcctgtagcc ggcctcgctg aggccggggc tgttccagat ccaaaaaccc ccgagcccgc
      841 ggggtcaccg cccccgccta cagtccaacc cattccgcat cctgtagccg gcctcgttga
      901 ggccgggggc gttttcccat aaacggtcgg tcctacaaaa ggaatacggt ttcatttctt
      961 cttccccctt gcaccgcgcg aactggataa aagcggcaac aactgctctc gacaacgatc
     1021 cattgcgctg atcgccagtt tcgcttgttc ccttgttgcg tcagccccag ggtagcggaa
     1081 agtgacggca tactgcgaca gcaattccaa atcatcgcgc agcaattctg tccgtgccgc
     1141 tccggtcgga cattgtgtca acaacaccac gagatcatgg accttgcgga tcgggcagtt
     1201 ggctgattcc agaatagcct tgatggtctt ctcgacgcac tgctgggcat ggaagcaggc
     1261 actgtcaaag tttgggcgct tccgcgcgcg gaattcccgg cgcgcggaaa tcgcgtcagc
     1321 ttcagccttg cataaccaag ctttaacggc gcttttcata cagcaccttt ccccgctcca
     1381 acaccgaaca gatgaagcca tcgcgacctt gcatgccgcg ccgcacttca cccggagttt
     1441 tgacaaccaa atccagcgga aacgatcgcg gaattcgtcg ccgaatttcc aacgcgctat
     1501 cggtggtgtt ttgcgttccc gccatcacga ccatcagatc cacgtcgctg tccgccgtgg
     1561 cccggccttc cgccatggag ccaaacagga tgacccgctc cggacggaac tgctccacca
     1621 gatgatccac gtactgctgg acctgccgca aaatcatggc gctacccctt ggcgttcttt
     1681 gcgtcttggc gagagggccg ggcgtctccg ctctcccagc gctactttct cggcaacaca
     1741 aatgccctct tcctggatgg ttttgaaaaa ataatctccg gcagcctttt tctcctcaaa
     1801 gcgcgccggg gtgatgggaa tcagcgtgaa cgccggcttg ggtctgacgt ttcgcagggc
     1861 gcggcggaag tcgcgggcgg cagccaattg gtcctcggcc ccttcggcca caatgcacaa
     1921 atccacgtcg ctgtccggtc gctgttcacc gcgggcatag ggaccgaaca acacaacacg
     1981 ctgtacaggc cgcgcttgcc ccaacgcacg caggcagtgc gccaatcctg ttgacggatg
     2041 ccgcaaagcc ggcgggatgt tttcgtttaa caccttcatg gcgccaccgc tgttttctcc
     2101 gctttattca aggcgctaaa tcctgcaagc cacgcggggt caccgccccc gcctacagtc
     2161 caacccattc cgccccctgt agccggcctc gctgaggccg gtgtcttttc ccataaacgg
     2221 tcagccctat aaattttctg catggcccag tcgggattga tagaaccgag aggtgcgtag
     2281 tgtccttggc gctcttcgcg tcttggcgag agtcccggtt ctccggcttg cggttgttcc
     2341 cgattgcgtt tcatttgcgc cgtttgcgcc acttgcgtta aatcggctgc cagccttggg
     2401 taggatatgg ccactgccag cgccgcgcgg taatccgtga gcaattcgtt gacccggtca
     2461 ggcttcataa atcaccttcc cgtggcgttg gatggattca cgaaaacggg gtttggtcac
     2521 cgcattcaca ttcaccaaat cgaacttcag cgcggtcggg acctccgact ccagccggtc
     2581 gtgcgccaaa ttcgtgtccg aatcggacca gttgtcggcc tcaatggcga gatccacatc
     2641 ggacgcggcg cgcgccaccc ccgccgcgcg tgagccatac agccaaatcc ggcgcaccgg
     2701 cttgtgcgcc gtaatcgcgg ctacaatcgg cgcaatcaac cgggcgtcca gttggacgga
     2761 acccaaggtg cgtcgggatt caggcctatc cgtgagattt gtcgtttgca gggctcgatt
     2821 catcgctgcc ctcccgcttt ggtctgaact ttcatccgct tttcgctcgc ggggtcaccg
     2881 cccccgccta cagtcaaagc cattccgcac gctgtagccg gcctcgccga ggccggaact
     2941 ttttcgcgtt gactcgcgta aaccgcggtt ggtgcgtccc agttcatggc cgcccttcct
     3001 tcgacttttt cttggcagaa atcacctttg acggggcgga ttttgtgctc cgcaaagcgg
     3061 caaccgtctt ttgttccact ccaaactgcc ggataaagcc atggcatcgt gcaaccagag
     3121 cggccgcatc ttggacgtct tctcttgctg acttcgcatc gccggcaaac atccagtcat
     3181 agtcagcgag ttggcggttt ccaaataagc gatgcagaat tttggcaaac tctttgtcaa
     3241 acaaacccgc ccggacaaaa cgggcgttaa acgccccaat tgtttgagcg tgcgatgaaa
     3301 aggccagtcc ctgagtcgcc aaaatcgctg ttacggcatg aaaagctgca tagtaggcgt
     3361 gggtggttgc ctcctcgaaa tatctggctt tcagttcgcg tctggccgcc gccaactttc
     3421 tccttgcctt agccagcatt tggcgcaaca cagttctgga tgcgacgcgg gtcacaactc
     3481 caccccttcc cgcagaatat tccagcccaa cggcgactgc tgtacgcgct cccattgctc
     3541 cggactgtag accagcgcgg tgcagagttg atcggtttcc gccaatagtt tcgcgcccgc
     3601 agccgtcacg gtttcgcgtt ctttctgcgt gggcgaactc aacaccacca gaaaatcata
     3661 gtcggaaccg gcatgggcat cgccacgggc acgcgaacca tacagtgcga tcaaccgcac
     3721 ctgatcagtc aagtcttggc gaatggcttg ggcataccgg cgggctagtc gcggtatcgc
     3781 acatgcagca ttcggtattc tccggctacg acgctggcgt ttcatcgtcg gcgttcctcc
     3841 ctgaaaacct ctgtgcacag ttcgagccat agttgtatat atagaagatc atctattccc
     3901 cctgctgtgt caaggaacta accaaattct aataaaccgt ttttctcggc attattcgcc
     3961 atattttgcg aatattccgt tttttctggg cgaggccttt tggcctattg tcaacccttt
     4021 ggcctgaagc gggttggcag caggcgtgcc gtcttcgatg actcaccaga cacggggaca
     4081 gcaaaccgga aaacattaac gcaaaagacg caagcgccgc aaaaggacga acagccgcgc
     4141 tggggctggt aacagtggcc ttcaaaacag cggcatcatg ccaaccctca atgggtaaag
     4201 atgttttcac ccgtgttgat gaatgattca gtttaggctc ttttgcgggc ttttgcgacg
     4261 tcagcgacgc ttgcgttaat tctggctttc ttggcggcnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnct ttcttggcgg ccttggcggc ttggcgagac gagtagatca
     4441 acacaaaagg cgcaggcaat gcgaaaagca ataatcacta cgtaaattaa tgattcgata
     4501 aataattaac acattattat tggctgatta ctattattga tttcctgccg ggctttacgg
     4561 ctattcgccg cattattacg ggcagtttag ctattcacgc cagggttttc caaggcgata
     4621 ttcggccagc cgcttttcaa tctccgccgc caattccttg ccgccgtcgc gacgggccaa
     4681 acgggcgccg cgcagggcgg cttcaatcgc tccggcaaaa tcgccggcat tggcccgagc
     4741 ggcgccgagc gtatcccacg cggtcgcggc ctgcttgccc tcctcggaat tcgcggcttt
     4801 ttccgcccat tccaaggcct tggcaggcca ttcttcttcg cggtggtggt tgatttttgt
     4861 tggcttggcg gtggcgagca gccaagccat gttgttgaag gccacggcgt tggtgggttc
     4921 gcggcgggca aactccagga agtagccata ggcatagcca cgggcgccat cacgccatat
     4981 ttgggcgtag caagggaaaa gatcggccca ggtcatgcgc ggctcgccgg cggcctcggt
     5041 catgcgtcgg gcgacggctt gggcttcttc gtcgaaagcg tcagcctcgt ggctggcgcg
     5101 cagatattcg gccaccacaa aggcgttggg gtcttcatcc aaggcaaggg cttccttgaa
     5161 agcgccgatg gcgccggcca ggtcgccacg ggcttgcatg cactgtcccc gcacccgcca
     5221 gagaaattcg cgctccaagg ggcgttgatc tttgagggct tccaacaggg atctgccgag
     5281 acccttctca aagcgaggcc aggcgtcatc ggtcagctcg gggaatccgc agcaattcca
     5341 gataaggcac agccggagga agctgtgggt ttcccagaac gagctgccct gctcataagc
     5401 tttggctgct tgggcccagt cctttttggc gaggtagccg tcgcccccga gcatgcaggc
     5461 cacggcgtgg ttgggattgc aggtgtaggc ccagatgccg aaatcatcgc ggctgcgcca
     5521 atggcgggac aggcgcagcg acatccccgc ccacgcggcg ataaccacgg cggcgacggc
     5581 cacattccaa cgcattttcc agccgcgcac gcgttggact tcggccaaag cggccactgc
     5641 cgccagcaac agaccgattt gcgggatata gagccagcgg gtggcgacgc gctcggcgcc
     5701 ggcccaaacc accccactca acgggaatag caggatggcg aaccacagcc agccccagag
     5761 cgccagcgga aagcgttttc gccatcgcca cgccccccac gtaaaccccc cgagtaccag
     5821 ggttgccccc atcagcaact cccaccacga gggcaggtcc gcttgcagcg gcatcagacg
     5881 cgccggccag agcactttgc tcaagtagaa caggtagtgc accgggatca tcatggcacg
     5941 gtgccatgcc ggcacatcca tcacggcctt ggtttccacg tggaagatga tgggcaacga
     6001 ggcgaaggcc acggccggca gccagaacgc ccatttttcc gcgaccagcc gccagccatc
     6061 tttccaaatg cgatcccaat ccgttcgtcc caagggccag acgtccagca gcacaaacgc
     6121 agccggcata atgatgacca cctgtttggc catgccgcca acgaccatgc acaaccaagc
     6181 gagccccaga aaaagtgccg ggtggcgcat acgggcggcg ggattttgac tggacgggcc
     6241 ggcttggtgc gcggcggtat aaaaccacag cccggccaac aggaagagac cgctcaggac
     6301 atccttgcgg gcggtgaccc aggccacact ttccacccgc gtgggatggc acgcccaaag
     6361 cagcgccgtg gccaaagcaa caccggcacg tcccccggtc cattgccaaa ccaagaggaa
     6421 caagagcccc gcattgagcg caaacagaat ggtatttgtc aggtggcagc tccaaggcgg
     6481 accgccggag atttccttgt cgagcatgaa gctcatccag agcagcggtg cccagtagtt
     6541 ttccggggcg gtggtgaagg ccaactggag gccttcgcgg gtgaagccat cccggaccac
     6601 gtcgctgttt tccacgtaca tggggtcgtc gagggtgacg aaatcgtagc ggatggtcgg
     6661 caaataaagg gcgaaggcta cgaccatcac cagcaggcaa gctcctgcca tccggccgcg
     6721 ttcactggct aaccaacggg tcatgccgcc acactctatg ctgtgtggtt ggcacgggca
     6781 actgttttga gcg
//
LOCUS       DAOPNP010000120         6697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_12583_length_6697_cov_7.445799, whole genome shotgun sequence.
ACCESSION   DAOPNP010000120 DAOPNP010000000
VERSION     DAOPNP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12583_length_6697_cov_7.445799"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..2922)
                     /locus_tag="PKI64_09460"
     CDS             complement(370..2922)
                     /locus_tag="PKI64_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01264.1"
                     /translation="MNPRPRRLGFAGLLLVSGLLVWLYAAMPLLRYFNQAIPHSHNHT
                     RTLAPTPLVAGDHLQLLYHFWLGLDAFTGRSPLFHNVYEFNLGNDAERVQPDLYYLPF
                     SLVYMALAPWAGHAAGWNAAGLASVLLGALGAGLLARRFTTSRSVALLAALLVTALPY
                     RWITLFSGSPTGYAMAFPPLLFYGLDRAIRDRAFSGGLLAGLMILCALMTDLHVFYFS
                     MLAAPPFALFSLWLAAPTWRDWPGAIRRAVWPLLPCAALVAVAAGVSALANRHLAGSV
                     MAGGRSLAEMANYSPPAHGLISASATGMANHAYVGLPLLLLLVVGMACWVWNLRRPAP
                     DAATTTLARRAVPFLLLATAGIIILGLGIHGPHEGIVIRVIRKIVPKYSMIRQPVKIY
                     CLLPVTLGPLLAILLAQVNHARRGWPRAALWLLCGGLAAGSFMLSMGMVYPNFDRLPG
                     PNAAYEAVAHDDTANAGRPPHALALPLWPGESHWASLYEYATMSSRVRLVNGYAPAVP
                     AGYFTNVFKKYESLNQGHATDDQLDDLLAMGVRHLIFHANAFPEKVSPFPAAATLRAL
                     TGHPRLALLADDGLTFAFRILPKHPIEHTPHANWTEALYSAALQWRWDPPKEIPVNDS
                     TRLTLRAPVFPAPDLRYLLRLAEGSTQPLVIPPAADGVASVTHPVPRLPDWLQAELPS
                     PMGGLAHAAAGPVQLPAALLTAGDLPAPDVDNAIHIPPALLFHTGHSTPGQPGVQFRP
                     DTVPAGRVLYGPNLPLPPGLYDITISYSFQDPIPGDIFRLLTVPARQPLVETLLDSAR
                     QEQQFAAVMLGADPICFEFHYTGKTSVTIRDIVLTPATLRLTPAGDKPATAP"
     gene            complement(2983..3726)
                     /gene="plsY"
                     /locus_tag="PKI64_09465"
     CDS             complement(2983..3726)
                     /gene="plsY"
                     /locus_tag="PKI64_09465"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOE01265.1"
                     /translation="MSPSNIAIGKIVLEYVRSQPECGTNFIAFGWPFGGGQRILWRMI
                     TNSPLHLTLLVVAAYLLGAVPNGLLIARLKGINLQQVGSGNIGATNVFRCVGKGWGIL
                     AFVLDAVKGFVPAFALPLLVDSAPGWLGLACGVTAVAGHNWPVWLKFKGGKGVSTSAG
                     MLLGIAPAAVGIGFLVFAVTVVLTRWVSLGSILAAIAVPVAHVALHGTDNRLLTGALI
                     VMGVLVIWKHRTNIRRLLNGTEPRIVGQK"
     gene            3755..3967
                     /locus_tag="PKI64_09470"
     CDS             3755..3967
                     /locus_tag="PKI64_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOE01266.1"
                     /translation="MSLPSGGEWIIILIIAVLIFGRRLPEIARGIGKSITEFKKGIKD
                     VETDIKSVDEPAKKADEVPQKEEPKP"
     gene            complement(3964..4773)
                     /gene="tatC"
                     /locus_tag="PKI64_09475"
     CDS             complement(3964..4773)
                     /gene="tatC"
                     /locus_tag="PKI64_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOE01267.1"
                     /translation="MPFGTRSEYSNDKTDHFSANPSAMSFGAHLDELRLRLGQALGGF
                     VVAFIASLSVGKWFAGVILSPYKVAMEAAGIEVRLQAVQPAEPFLVYMKAAMVLAALI
                     SSPWVFYQLWAFISAGLHKHERRYVHMVAPASAALFAVGVLFFLLVIAPWVFKFFMKF
                     DLGIDYLTYQPGVGKTADFILVLALVFGIAFQTPIAIVFAERMGLVSFAALCEARKYV
                     FLGTFVIGAVLTPPDVISQVALAVPLYILYEGGLLVCRFWQKKRKIEPSAT"
     gene            complement(5016..5804)
                     /locus_tag="PKI64_09480"
     CDS             complement(5016..5804)
                     /locus_tag="PKI64_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01268.1"
                     /translation="MALINVCALNCAYGDDTNEAFAVMSSSSWVSQVYRDANGNELIA
                     IIHLSFPDKAEFNIQEYVRRQGEKANPDVWSLTKSIEGLVGNEITISISSSKLTLRYK
                     QDETSWPLEYRRQSVRYVQPNHDMAEALQKRIDAIGQWMSYSVNNEMWDPLIWTLAIA
                     EDDTFSMQMYSYGQPAAPNDWSLMLKLVGDIRYELGALDIKHVFNVPSGSLEQWHGET
                     PLLKEKVELLFDRTDSALFVVFPADVKDNSPNMVLTFHRNGVVP"
BASE COUNT         1584 a         1877 c         1968 g         1268 t
ORIGIN      
        1 ggaatggttg agggatgaaa ctttcccgcc gggaacggct ggaaggtctg cgaccgttcc
       61 agccctacaa tccaatcccg caagcggttt ttggggtcac gacactctca aacgaatcgt
      121 ctggttgcat ccataatgcg gtacgttacg gcgtggtagc cggattgagt ttcgccttgg
      181 gacacccaac aattgcgctg attttgtgag gtcttttgta gggcgggaat ggttgaggga
      241 tgaaaccttc ccgccgggaa cggctggaag gtctgcgacc attccagcgc tacaattcag
      301 tcggaatcgg tttttggggt caggacaccc tcaaacgaaa tcgtctggct gcacccgtaa
      361 cgtggttcgt tacggcgcgg tagccggctt gtcgccggcg ggggtcaggc gcagtgtggc
      421 gggggtcagc acaatgtccc gaatggtgac cgaggttttc ccggtgtagt ggaactcaaa
      481 acagatgggg tccgcgccaa gcatgacggc agcaaactgt tgttcctgcc gggcggagtc
      541 aagcaaggtt tccaccagcg gctgccgggc gggcacggtg agcaggcgaa aaatatcgcc
      601 ggggatgggg tcttgaaatg agtaggagat ggtgatgtcg tagagcccgg gggggagggg
      661 cagatttggg ccatagagga cgcggccggc cggcacggta tcgggccgga attgcacgcc
      721 cggttggccg ggcgtggagt ggccggtgtg gaaaagcagg gcggggggga tgtggatggc
      781 attgtccaca tcgggcgcgg ggaggtcgcc cgcggtcagg agcgcggcgg gaagttgtac
      841 cgggccggcg gcagcatggg ccagtccacc catggggctg ggcagttcgg cttgcagcca
      901 gtcgggcagg cggggtacgg gatgggtcac gctggccacg ccatccgcag cgggggggat
      961 gacgagcggc tgagtggagc cttcggccaa ccgcagcaaa tagcgcaggt ccggcgcggg
     1021 gaatacgggg gcgcgcagcg tcaggcgggt ggagtcgttg acggggattt ccttgggcgg
     1081 gtcccagcgc cattgcaagg cggcggaata gagcgcctcg gtccagttgg cgtggggggt
     1141 atgctcaatg gggtgtttgg ggaggatccg gaaagcaaag gtcaggccgt catcggccag
     1201 cagggccagg cgggggtggc cggtcaacgc gcgcagcgtg gcggcggcgg ggaatgggga
     1261 cactttttca gggaaggcgt tggcgtggaa aatcaggtgg cgcacgccca tggccagcag
     1321 gtcatcgagt tgatcgtcag tggcatggcc ttggttgagc gactcgtatt tcttgaagac
     1381 attggtgaag tagccggcgg ggacggcggg ggcgtagccg ttgacgaggc gcacgcgcga
     1441 cgacatggtg gcgtattcgt agaggctggc ccagtgcgat tcaccgggcc acaacggcaa
     1501 ggcgagggcg tggggggggc ggccggcgtt ggcggtgtcg tcgtgagcca cggcttcata
     1561 agcggcgttg gggccgggca gtcggtcaaa attgggatac accatgccca tggatagcat
     1621 gaacgatccc gcagccagcc cgccgcagag cagccaaagc gcggcgcgcg gccaaccgcg
     1681 acgagcgtgg ttcacctgcg caagtaagat ggccagtagc gggccgagcg tcactggcag
     1741 caggcaatag attttcaccg gctggcggat catcgaatat ttcggcacaa ttttgcggat
     1801 gacgcggatg acgatgccct cgtgggggcc gtgaatgccg agtcccaaaa taatgatgcc
     1861 cgcagtggcc aacagcagga aaggcaccgc gcggcgggcc agcgtggtgg tggcagcatc
     1921 cggtgcgggt cggcggaggt tccagaccca gcaggccatg ccgaccacca gcaacagcag
     1981 caacggcagg ccgacatacg catggttggc catgccggtg gcggaggcgg aaatcaaacc
     2041 gtgggcgggc ggggaatagt tggccatttc ggcgaggctg cgcccaccgg ccatgacgga
     2101 gcccgccaag tgacggttgg caagcgcgct cacccccgcc gccaccgcca ccaacgcggc
     2161 gcagggcagc agcggccaca ccgcgcggcg aatggcgccg ggccaatccc gccacgtggg
     2221 cgcggccagc cacaacgaga acagcgcaaa cggcggcgcg gccagcatgg aaaaatagaa
     2281 tacgtgcaaa tcggtcatca gcgcgcacag aatcatcagc cctgccagca ggccgccgct
     2341 gaaagcgcgg tcgcggatgg cgcggtcaag gccgtaaaac agcagcggcg gaaacgccat
     2401 ggcgtaacca gtgggcgagc cggagaagag cgtaatccaa cggtagggca gcgcggtcac
     2461 cagcagcgcg gccagcagcg ccacggagcg tgatgtggtg aaccgccggg ccagcagccc
     2521 tgcgccgaga gcacccagca gcacggaggc caagccggcc gcattccagc cggcggcatg
     2581 accggcccat ggcgccagcg ccatatacac cagcgaaaag ggcagatagt agaggtccgg
     2641 ctgcacccgc tcggcatcgt tgccgagatt gaactcatag acgttgtgga acagcggcga
     2701 gcgtccggtg aaggcatcca gccccagcca aaagtggtag agcagttgca ggtggtcgcc
     2761 ggcgaccagc ggcgtgggtg cgagggtgcg ggtgtggttg tggctatggg gaatggcctg
     2821 gttgaaataa cgcagcagcg gcatggccgc atagagccac accagcagac cggacaccaa
     2881 gagcaggccg gcaaacccca aacggcgagg acgcggattc atgcggccag aatagaaagc
     2941 cgtgcggggc gaggcaagcc aaagaccgcg cgccggcggg ccttactttt gccccacgat
     3001 gcgcggctcg gtgccgttga gcaaacggcg gatgttggtc cggtgcttcc aaatcaccag
     3061 tacgcccatc acaatgagcg cgccggtcag caagcggttg tccgtgccgt gcagggccac
     3121 gtgcgccacc ggcacggcaa tggccgccaa aatggagccc agcgacaccc agcgcgtcag
     3181 cactaccgtg acggcaaaca ccaaaaaccc gatgcccaca gcggccggcg caatgcctag
     3241 cagcatcccc gcgctggtgc tgacaccctt gccgcccttg aacttcaacc acacgggcca
     3301 gttatgcccg gccaccgccg tcacgccgca ggccaaaccc agccaaccgg gcgcgctgtc
     3361 caccagcagc ggcaacgcaa acgcgggcac aaagcctttc acggcatcga ggacaaacgc
     3421 caaaatgccc cagcctttgc ccacgcaccg gaatacgttg gtggcgccga tattgcccga
     3481 gcccacctgc tgcaagttga tgcccttgag ccgggcaatt agcaggccat tgggcaccgc
     3541 gccaagcaaa taggcggcca ccaccagcag cgtcaaatga agcggcgagt ttgtgatcat
     3601 gcgccacaag atgcgctggc cgccgccaaa tggccagccg aaagcgatga aattcgttcc
     3661 acattcgggt tgactccgca catattccag tacaatcttt ccgatggcaa tattcgaagg
     3721 gctcattatc atgattaatc caacgttggc gtttatgagt cttcccagcg gaggggagtg
     3781 gatcatcatc ctcatcatcg ctgtcctgat cttcggccga cgcctgccgg aaatcgcccg
     3841 gggaatcggc aaaagcatca cagagtttaa aaagggcatc aaggacgtcg agactgacat
     3901 caagtccgtt gacgaaccag ccaagaaggc cgacgaagtc ccccaaaagg aagagcccaa
     3961 gccctaggtc gcggacggtt cgatttttcg cttcttctgc cagaatcggc agaccagcaa
     4021 gccgccctcg tacagaatat agagcgggac cgccagtgcg acttgggaaa tcacatcggg
     4081 cggcgttaaa accgctccga taacgaaggt cccgagaaac acatatttcc gcgcttcgca
     4141 aagcgccgca aatgaaacca gccccatccg ctcggcgaac acgatggcga tgggcgtctg
     4201 gaaagcgatg ccaaacacca gcgccagcac gaggatgaag tccgccgtct tccccacccc
     4261 cggctgatag gtgaggtagt caatcccaag gtcgaacttc ataaagaatt taaacaccca
     4321 tggcgcgata accagcagaa agaacagcac gccgacagcg aacaatgccg cgctggccgg
     4381 ggcgaccata tgcacatacc gtctttcgtg cttgtgcagc cccgccgaga taaaggccca
     4441 tagctgatag aagacccacg gcgaactgat cagcgccgcc aagaccatcg ccgccttcat
     4501 gtagaccaaa aatggctccg ccggctggac agcctgcaat cgcacctcga tgcccgccgc
     4561 ctccatcgcc accttgtacg gcgacaaaat gacccctgcg aaccatttgc ccaccgatag
     4621 cgaagcaata aacgccacca caaatccccc cagcgcctgc cccagccgca gccgcaactc
     4681 gtccaaatgc gccccaaaac tcattgccga ggggttggca gagaaatgat cggtcttgtc
     4741 atttgaatac tctgatctgg ttccaaaggg catatcgcca tcctcccttt tgggcaccct
     4801 ccagtcgatg caaaaaatga agcaaatccc ccccgaaccc gcctcaaacc aaaaggctaa
     4861 ttattctgga cactaccatc tgcggtacag acggtcagga aagtggtcat gattttcccg
     4921 gtgaaaataa ggaaaggcga tccgtttttg aaaaacggct caaaggcggt ttttgtgttt
     4981 gaccggaacc tctaatgggc gcccccttac cttacctacg ggacgacccc attcctgtga
     5041 aacgtcaaca ccatgtttgg cgaattatct ttaacgtctg cggggaaaac aacaaataaa
     5101 gcactatcag tcctatcgaa aagcaattcc accttttctt tcagtagcgg ggtttctcca
     5161 tgccactgct ctaggcttcc agagggaaca ttgaatacat gtttgatgtc aagtgcccca
     5221 agttcatacc tgatgtcgcc aactagcttc agcattaatg accaatcatt tggcgctgct
     5281 ggctgcccat agctatacat ttgcatagaa aaagtatcgt cttccgcaat agcaagcgtc
     5341 catatcagtg gatcccacat ctcgttattc acagaatatg acatccactg gcctatcgca
     5401 tcaattcgct tttgtaatgc ctccgccatg tcatgattag gttgcacata tcgaaccgat
     5461 tgcctgcgat attctaaggg ccaactcgtt tcatcttgtt tataccgaag ggtcagtttg
     5521 ctagagctta tcgatatagt aatttcattg ccgacaagcc cttctatact ttttgtcaaa
     5581 gaccatacgt caggattagc cttttcacct tgccttctca catattcttg aatgttgaat
     5641 tccgccttgt cgggaaatga taaatgtata atcgcgatta gttcattgcc atttgcatca
     5701 cgatatactt gggacaccca agagcttgac gacataacag caaaagcttc atttgtgtca
     5761 tcaccgtatg cacaattaag tgcgcacaca ttaatcaagg ccatattata acatatatgt
     5821 acaatatgta gaagatattt tctgatattc atatttaaac acatcgcgca ttccttaata
     5881 ttgtagaagc acaggatcat ttccgcagct ttctatcgga tggagtaaat tttgtggtga
     5941 gctccgtcct ggatgtagac ggacaggcgg ttcaatcaat ggaattgaag atactgcagc
     6001 caaccatcga ataaatgtat tgctattatt gccaggcggc agttcatacc gagaatttgg
     6061 ccagtgttga aatactccgt tgaagccact ttgctcagcg aacccgtatg tggtcgcatt
     6121 tgccgatact ctcccccatt cagacgacac atcgctttta taaactacaa taacgtgttc
     6181 agtggccctt gatcttgcgt gaatagttga aatgtcaaca aaaattatac ctgatggaac
     6241 accaaaaaaa cacctttgaa ccgcccaatc accgccatcc ccatttttta aaagctccca
     6301 cgctcctgtt cgagaatatc cagatctaag cagctcaaag gtcgtgtatt gaactccttg
     6361 ttgcaactca atgattgctg tcgaagggat cgaagggatt ggctcccatc tttcaattga
     6421 caaatggcca aaccattggt tcccacatat atttactaat cgactactta tggcaataaa
     6481 ttgtttggcg tgtcccagcg gcgaatggcc agttcctgag aacacaggcc agactaatag
     6541 agaaaatacc gctgtaattg ataacatatg gaaacgagtg tgcctcacga tagggcctcc
     6601 atatccgcgt gaatttgcca gctttgtgct ctgagcttgc cttggtcgta attcacggct
     6661 tggggatcta cttgggccag atccatgtct gcgagta
//
LOCUS       DAOPNP010000121         6580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_12833_length_6580_cov_8.786054, whole genome shotgun sequence.
ACCESSION   DAOPNP010000121 DAOPNP010000000
VERSION     DAOPNP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12833_length_6580_cov_8.786054"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKI64_09485"
     CDS             complement(<1..779)
                     /locus_tag="PKI64_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE01269.1"
                     /translation="MRVIWAIALQALRLTFRSRIVLVLALLLGAAICLLPATIRSDGT
                     PAGLIHLHLAYTLGSASFLLTLATLWAGCAAIAQEADDKTLQLLLVKPVPRAHVWLGK
                     WLALLLLNALLLALAGSTSALTLRYKLCHGGFAPEDLAQARATTLTALATTTAPLPDI
                     TADVEAEYTHLRARRALPPDAPPAVVRDSIRRTRLAQLYSLAAGATRLWSFPQPPAAT
                     RQLVQFSCDSSIPGAVEIQGTVRLHSGDYTASRDFSAMAGTP"
     gene            complement(776..1060)
                     /locus_tag="PKI64_09490"
     CDS             complement(776..1060)
                     /locus_tag="PKI64_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01270.1"
                     /translation="MLQFSSMRKDIGWQEHQEDAKYEIRVTFDGGGRIRWRRITKRME
                     RWETFEPTPAHWQLLLEKMQARYVRRQAPYKDLQLVERLAAANPLPPAEA"
     gene            1085..2323
                     /locus_tag="PKI64_09495"
     CDS             1085..2323
                     /locus_tag="PKI64_09495"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006286336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOE01271.1"
                     /translation="MNSQASGAVPEPSFEPATLRPHFAILNRELAWLDNAATTQRPEA
                     VLKAMAQFYRRHNANPRRSVHRLGAEATTAYERARLAVAQFIGARSADEVVFTAGTTA
                     ALNLVAAGMARWEIGPGDEIWVSAAEHHSNWVPWQQVARAMNAALRVIPLLPDGRLDV
                     AAFRKGLAARRTKWVAVTWVSNVLGAVNDVAEICAAAHAAGARVVVDGAQGVAHLPTN
                     VAQMDCDFFAFSGHKMYGPFGIGVLWGRQEWLTQMEPLIFGGEMISRVDERSAQWAEV
                     PTRFEGGTPNAAGAVGLAAAIEWLRGLPAAAHAHVNQLAREAAEQLAALPGVQVWGPR
                     EGRVALVSFSVDRVHAHDVAQVLDDRGVAVRAGHLCAQPLLRRLGTPSVVRASFAPYN
                     TRDEVARLVAGVRAAQELFT"
     gene            2320..2745
                     /locus_tag="PKI64_09500"
     CDS             2320..2745
                     /locus_tag="PKI64_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOE01272.1"
                     /translation="MNGIYQEKLLAHARAPAGAGTIPPGTEDAEVFNSACGDEIRVKL
                     TWTPEGALAGLTHETHGCAVCQAAASLAVPRLVGKTAAEIAGLAAAFYSRLGQSGFEE
                     AWGDFQMFNGLEKYPARLACARLIWQAVERALAPKLGRP"
     gene            2742..3755
                     /gene="amrS"
                     /locus_tag="PKI64_09505"
     CDS             2742..3755
                     /gene="amrS"
                     /locus_tag="PKI64_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOE01273.1"
                     /translation="MKTARFWEAAENSAVRCVLCPHRCRLAVGARSRCFGRVNHGGTL
                     MAETWGQPMALQVDPIEKKPLYHFLPGSRILSVGTLGCNLACQFCQNWELSHPTPRRQ
                     ATGRPTLPEEIVDMAVHNQLPAIAATYNEPAVWAEYALDIAAAAHARGIKMVAVTAGY
                     FSPESRGEFFGQMDAANVDLKSLRDDFYRRYCGARLTPVLDTLLAIRQETSCWLELTT
                     LLIPGLNDSDAELDELTKWVTENLGPDVPQHFSAFHPAGERQDLPSTPLATLRRAERV
                     ARANGLRHAYLGNVAAPDVATTRCASCGATLITRDGFAARSVGLDSGGSCRACGTPSP
                     GVW"
     gene            complement(4160..4975)
                     /locus_tag="PKI64_09510"
     CDS             complement(4160..4975)
                     /locus_tag="PKI64_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01274.1"
                     /translation="MRKLVLLSIIGLYAGAWVAGAADANLELNILSAYVNYGQVGNDE
                     AVFQPSFDVAGPYGFGFNLWANMDLTDNARSWWPNTAGEWSELNLELNWTAPLEGPVS
                     LTLGGVYFAYPQQASEVLIPEDSGEVIVQPHPADGGYEVYILVEAEDVLLSPRAQFYH
                     DLGNADDWHAELAIGHTIELTAALSLDLTAILGMAGKYYVEDNFGSDAGDAITHAQFE
                     ATLNYALNEKITLGLTGAFMTILDSDIRDDVKESDIYPDVDFFFGGLTIGYTF"
     gene            complement(4972..>6580)
                     /locus_tag="PKI64_09515"
     CDS             complement(4972..>6580)
                     /locus_tag="PKI64_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOE01275.1"
                     /translation="PALLKDHGVEENFPPEVQRAARQVATTVRRTHGPRRVDLRDRLI
                     LTIDPASAHDQDDAISIARLPDGCWQLGVHIADVAAYVEPDSAIDREARRRGNSTYLV
                     DRVIRMLPRDLTVKVCSLRPNEDHLAHTVELIFDDAGRLHQSKTYRSLIRTRAALSYE
                     AVQRYFDTGAWPEIAPEIRATVDHLRHLAGQIRARRFEAGALDFAMPEVQCILDAQGT
                     PTGFQRRDAIEAYVLIEECMLAANQVVARKIRAAGLPAPYRIHLPPTPEKWARMASEL
                     HALGIRTTPARAADLNRIARRVAGQPAEHMVTLAMLRNMPRAIYAADCQPHFGLGFDC
                     YAHFTSPIRRYPDLVLHRVLLAIEEGRPPPYSAEEIASLARHCSDTEAESAELEAESI
                     LLKRIQYYAAQMAQGRTGPFAGSIIALNPKGLLVELNDSLQTGLLPYRALGRQQYVLA
                     ADGLAATALRGKTYRLGQTLHVQLATVDEKRRRIDFAPADLPTPRAPAKKKCATPDND
                     VDTPQPKTEKHTPTGQRVSGSQRRKTK"
BASE COUNT         1145 a         1819 c         2396 g         1220 t
ORIGIN      
        1 ggcgtgccgg ccatggcgga aaagtcgcgg ctggcggtgt agtcgccgct gtgcaggcgc
       61 acggtgcctt ggatttccac ggcgccgggg atggaggagt cgcaggaaaa ttgcaccagt
      121 tggcgcgtgg cggcgggcgg ttgcgggaag gaccacaagc gggtagcacc ggcagcgagg
      181 gagtagagct gggcgaggcg cgtgcggcgg atgctgtcgc gcacgacagc gggcggggcg
      241 tcggggggga gggcgcggcg ggcgcggaga tgcgtgtatt cggcttcgac atcggcagtg
      301 atgtcgggca ggggggcggt ggtggtggcg agcgcggtaa gcgtggtggc gcgagcttgc
      361 gcgagatcct ccggcgcgaa gccaccgtgg catagcttgt aacgcagggt tagtgcagat
      421 gtgctgccgg ccagcgcgag caacagtgcg ttcagcagca gcagcgcgag ccatttgcca
      481 agccagacat gcgcgcgcgg cacgggtttg accaagagga gttgcagggt tttgtcgtcg
      541 gcttcctggg ctatggctgc gcagccggcc catagcgtgg caagggtcag cagaaacgag
      601 gcgctgccga gggtgtaggc caggtgcagg tgaataaggc cggcgggggt gccatcgctg
      661 cgaatggtgg cgggcagcag gcagatggcc gcgcccagca gcagggccag caccagcacg
      721 atccgcgagc ggaacgtcag ccgcaacgcc tgcaacgcga tggcccaaat gactctcatg
      781 cttccgcggg gggcagcggg ttggcggcgg cgagccgctc cacaagctgc aaatccttgt
      841 agggggcctg gcgtcggacg tagcgggcct gcattttttc cagcaaaagc tgccagtggg
      901 cgggggtggg ttcaaaggtt tcccagcgct ccatgcgttt ggtgatgcgc cgccagcgga
      961 tgcgcccgcc gccgtcaaac gtgacgcgga tttcgtattt ggcgtcttct tggtgttcct
     1021 gccagccgat gtccttgcgc atggacgaaa actgtagcat gatgatgccc gagggcaagg
     1081 tgtaatgaat tcacaggcgt caggagccgt accggagccg tcgttcgagc cggcgaccct
     1141 gcggccgcat ttcgcgattc tgaatcgcga gctggcgtgg ctggataacg cggcgaccac
     1201 gcagcgcccg gaggccgtgc tcaaggcgat ggcgcagttc taccggcggc acaacgccaa
     1261 cccgcggcgc agcgtgcacc ggctgggggc cgaggccacc acagcctatg agcgcgcacg
     1321 gctggccgtg gcgcagttca tcggcgcgcg gtcggcggac gaagtggtgt tcaccgccgg
     1381 cacgacggcg gcactcaatt tggtggcggc ggggatggcc cgctgggaga tcgggcccgg
     1441 cgacgagatt tgggtttcgg cggcggagca ccatagcaac tgggtgccgt ggcagcaggt
     1501 ggcgcgggca atgaatgctg cgctgagggt gatcccgctg ctgccggatg ggcggttgga
     1561 tgtcgcggct ttccgcaagg ggttggccgc gcggcgaacc aagtgggtgg cggtgacgtg
     1621 ggtctccaac gtgctgggcg cagtgaatga cgtggcggag atttgcgcgg cggcccacgc
     1681 ggcaggggcg cgcgtggtgg tggatggcgc gcagggcgtg gcccacctgc cgacaaacgt
     1741 ggcgcagatg gattgcgatt tcttcgcgtt ctccggccat aaaatgtatg gcccgttcgg
     1801 cattggcgtg ctgtgggggc ggcaggaatg gctaacgcaa atggagccgc tgattttcgg
     1861 cggggagatg attagccggg tggatgagcg gtcggcgcaa tgggcggaag tgcccacgcg
     1921 cttcgagggc ggcacgccta atgcggcggg cgcggtgggg ctggcggcgg ccattgaatg
     1981 gctgcgcggt ctaccggcgg cagcgcatgc gcacgtcaat cagttggcgc gggaggcggc
     2041 ggagcagttg gcggcgctgc cgggcgtgca ggtgtggggg ccgcgggagg ggcgggtggc
     2101 gttggtgagt tttagcgtgg accgagtaca cgcgcacgat gtggcgcaag tgctggatga
     2161 ccgcggcgtg gcggtgcgcg cgggccactt gtgcgcgcaa ccgctgctgc ggcgtttggg
     2221 tacgccatcg gtggttcgcg cgagttttgc gccgtataac acgcgcgacg aagtggcgcg
     2281 gctggtggcc ggtgtgcgcg ccgcgcaaga gttgttcaca tgaacggaat ctatcaggaa
     2341 aaattgttgg cccacgcccg tgcgccggca ggtgcgggga cgataccgcc gggcaccgag
     2401 gatgcggagg tgttcaactc cgcctgcggc gacgaaatcc gcgtgaagct gacgtggacg
     2461 cccgaaggcg cgttggccgg cttgacgcac gaaacgcacg gctgcgcggt gtgccaggcg
     2521 gcggcttctc tggcggtgcc gcggctggtt ggcaaaacag cggcggagat tgccgggttg
     2581 gcggcggcgt tttattcgcg gctcgggcaa agcggttttg aagaggcatg gggcgatttt
     2641 cagatgttca acggactgga gaaatatccg gcgcggctgg cctgcgcgcg actgatctgg
     2701 caggccgtgg agcgcgcgct ggccccgaag ctagggcggc catgaaaact gcgcgctttt
     2761 gggaagcagc cgaaaacagc gcggtgcggt gtgtgctgtg tccgcaccgc tgccggttgg
     2821 cggtgggcgc gcggagccgc tgcttcgggc gggtgaatca cgggggcacg cttatggccg
     2881 aaacctgggg tcagccgatg gccctgcaag tggaccccat cgagaaaaag ccgctctatc
     2941 attttttgcc gggtagccgg atcctttcgg tgggcacact ggggtgcaat ttggcgtgcc
     3001 agttttgcca gaactgggaa ctgagccatc ccacgcctcg ccggcaagcg actggtcggc
     3061 cgacgctgcc ggaagagatc gtggacatgg cggtgcacaa ccagttgccg gccattgcgg
     3121 cgacgtacaa cgagcctgcg gtgtgggcgg aatatgccct cgacatcgcg gccgccgccc
     3181 acgcgcgcgg catcaaaatg gtggcggtga cggccggata tttttcgccc gaatcgcgcg
     3241 gcgaattttt tgggcaaatg gacgcggcca acgtggattt gaaatccctg cgggatgatt
     3301 tttatcggcg gtattgtggt gcgcggctga cgccggtgct ggatacgctg ttggccattc
     3361 ggcaggaaac ctcctgctgg ctggagttga ccacgctcct gattcccggc ctcaacgatt
     3421 cggacgcgga gttggatgaa ctgaccaagt gggtgacaga aaatcttggc ccggatgtgc
     3481 cacagcattt cagcgcgttc catccggcgg gcgagcggca ggatttaccg tccacgccgc
     3541 tggccacctt gcgccgtgca gaacgcgttg cccgcgcgaa cgggttgcgg catgcgtatc
     3601 tgggcaatgt ggccgcgccg gatgtagcca cgacccgctg cgcgtcgtgc ggggccacgc
     3661 tcatcacgcg cgacggcttc gccgcccgga gcgtcgggtt ggattccggc ggctcctgcc
     3721 gggcatgcgg cacacccagc cctggcgtct ggtaagaaca aaaaaacaca cgccggaaac
     3781 cgacgcgtgt tgcggggatt tgtagcaccg aaacggccga agatattcct gcctttccca
     3841 gcgggaaggt ttcctccctc aaccattcgc accccacaaa agtccgataa aaaaaacagc
     3901 tacgaccgcg tccagttgta ttttggagtt gaaatggtcg tattcctttc tagaacaaca
     3961 tgttacattt tattttgtgg cccttgattt cacttataat tataagtgaa atcaaccgtt
     4021 tttatctggc aaatctgatt atttcggata tgtgtcagaa tgttgtgggc agcgaagtta
     4081 ttgggtgtcc caatgaggag ctcaggccat aaaaaaacgc gctccggatg ccggagcgcg
     4141 ttgcgtgacg cggttggcgc tagaacgtgt agccgatggt caagccaccg aagaagaaat
     4201 ctacatcggg ataaatgtca gattccttga cgtcgtcgcg gatgtcgcta tccagaatgg
     4261 tcatgaaggc gccagtgagg ccgagggtga ttttctcgtt gagcgcgtag ttgagcgtgg
     4321 cctcgaactg ggcgtgggtg atggcatcgc cggcatcgga gccgaagttg tcttccacgt
     4381 aatacttgcc ggccatgccg agaatggcgg tcaggtccag cgacagcgcg gcggtcagct
     4441 caatcgtgtg gccgatggca agttccgcat gccagtcgtc ggcgttgccg aggtcgtgat
     4501 agaactgggc gcgcggcgaa agcaggacat cctcggcttc gacgaggata tacacctcgt
     4561 agccaccgtc ggcgggatgg ggctgcacga tgacttcgcc ggaatcttcc gggatgagca
     4621 cttcgctggc ttgctgcgga taggcgaaat agacgccgcc cagtgtcagc gagacggggc
     4681 cttccaaggg cgcggtccag ttaagttcca agttcagctc gctccactcg ccggcagtgt
     4741 tgggccacca agagcgggcg ttatccgtca ggtccatgtt ggcccacaga ttgaagccaa
     4801 agccgtaggg cccggcgaca tcgaagctgg gctggaatac cgcttcgtcg ttacccactt
     4861 gtccgtagtt gacgtaggcg gacaagatgt tcagttccag gttggcgtct gccgcaccgg
     4921 cgacccatgc tccggcatac aacccgatga tgctcaacag taccagtttc ctcattttgt
     4981 ctttctcctt tgcgagccac tgacacgttg gcccgttggg gtgtgttttt cggtttttgg
     5041 ctgcggtgtg tcaacgtcat tatcgggcgt tgcgcacttt tttttggcag gggcgcgggg
     5101 ggtggggaga tcggcaggcg cgaaatcaat gcgtcgccgt ttttcatcca cagtggcgag
     5161 ttgcacgtgg agggtttggc cgaggcggta ggtcttgccg cgcagcgcgg tggcggcgag
     5221 gccgtcggcg gccagcacgt actgttggcg gccaagtgcc cgatacggca acaggccggt
     5281 ttggagggaa tcgttcagtt ccacgaggag gcctttggga ttcagcgcga taatgctgcc
     5341 ggcgaagggc ccggtgcggc cctgcgccat ttgcgcggca taatactgga tacgtttgag
     5401 caaaatggat tcggcttcca actcggcgga ctccgcttca gtatcggagc aatgacgggc
     5461 caacgaggcg atttcttcgg cggagtaggg cggggggcgg ccttcctcga tggcgaggag
     5521 aacccggtgc agcaccaaat cgggatagcg ccggatgggc gaggtgaaat gcgcgtagca
     5581 gtcgaaaccg aggccgaagt gcggctggca gtccgcggca tagatggctc gcggcatgtt
     5641 gcgcagcatg gcaagcgtga ccatgtgctc cgccggctgg ccggccacgc ggcgggcaat
     5701 ccggttcagg tctgcggcgc gcgcgggcgt ggtccggatg ccgagggcgt gcaactcgct
     5761 ggccatccgc gcccattttt ccggcgtagg cggcaggtga atgcggtatg gcgcggggag
     5821 gcccgccgcc cggattttac gggccacgac ctgattggcc gccaacatgc actcttcgat
     5881 cagcacgtag gcctcgatgg catcgcgccg ctggaagccg gtgggtgttc cttgggcatc
     5941 caggatgcac tgcacttccg gcatggcgaa atcgagcgcg ccagcctcga aacggcgcgc
     6001 gcggatttgc ccagccaaat ggcgcagatg gtccaccgtg gcacggattt cgggggcaat
     6061 ttccggccac gcgccagtgt caaagtaacg ctgcacagct tcatagctga gcgcggcacg
     6121 ggtgcggatg agcgagcggt aagttttgct ttggtggagc cggccggcgt cgtcgaagat
     6181 cagctccacg gtgtgggcca agtggtcttc gttggggcgc agactgcaca ccttgacggt
     6241 gagatcgcgc ggcaacatgc gaatgacgcg gtccaccaga taagtgctgt tgccgcggcg
     6301 gcgtgcctcg cggtcaatag ccgagtcggg ttccacatac gcggcgacat ccgcgatgtg
     6361 tacgcccaac tgccagcagc catcgggcag gcgcgcgatg gaaatggcgt cgtcttggtc
     6421 gtgcgcagag gcggggtcaa tggtgaggat cagacggtca cgcaaatcca cgcggcgcgg
     6481 cccgtgagtg cggcgaaccg tcgtggcgac ctgccgcgcc gcgcgttgca cctccggcgg
     6541 gaagttttct tccacgccgt gatccttgag cagcgcaggg
//
LOCUS       DAOPNP010000122         6538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_12933_length_6538_cov_7.823230, whole genome shotgun sequence.
ACCESSION   DAOPNP010000122 DAOPNP010000000
VERSION     DAOPNP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12933_length_6538_cov_7.823230"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PKI64_09520"
     tRNA            complement(1..76)
                     /locus_tag="PKI64_09520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Lys,seq:ttt)
     gene            1708..>1925
                     /locus_tag="PKI64_09525"
     CDS             1708..>1925
                     /locus_tag="PKI64_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE01276.1"
                     /translation="MTYDVVVIGAGHAGCEAALAAARLGATVALVTMDPAATARMSCN
                     PSIGGIAKSHMVFELDALGGEIALNTDCT"
     assembly_gap    1926..2025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2026..3468
                     /locus_tag="PKI64_09530"
     CDS             <2026..3468
                     /locus_tag="PKI64_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE01277.1"
                     /translation="GTFLRGKIFVGKKVVSGGRLNEPAEDQLSLQLQDLGLRLERLKT
                     GTPPRLHIQSLDYSKMQEQPGEDPVPFFSWLCREQAGMFHVEQSAANPAVPTVVPRGT
                     LPLPSFTPWIPGSNQIPCWLTHTNPQTIEIIREHLADSALYGGLIEGTGVRYCPSIED
                     KVVKFAAHNDHHVFVEPEGRAVPEMYPNGTSNSLPLEVQKRMIHSIPGLEQAEFLAPG
                     YAIEYDFFDPRQLFHTLESKRVGGLYLAGQVNGTTGYEEAAAQGFMAGVNAVMYLTGR
                     EGITLKRHEAYIGVMIDDLVTKGTNEPYRMFTSRAEHRLLLRQDNAVFRLAPVADKLG
                     IVPTARRQIIHEQAQAIAAEVARLNETHEGGITLAQQLKRPGASYAALPGRRHDLAPA
                     VIEQVEFELKYQGYIEREWRQIEKAEQMAKQKIPADCDFHAISALRYESREKLSAIRP
                     VDLGQAGRISGITPADIAILSVWLKKQNGS"
     gene            complement(3608..4315)
                     /locus_tag="PKI64_09535"
     CDS             complement(3608..4315)
                     /locus_tag="PKI64_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01278.1"
                     /translation="MKTAPDPLQNPRFADLLATLRAQPPPSPSPDFTRQTLARARQAE
                     IRHMPWLLRVARLAASIAILLGLGLWFTQRPPALNTQPTAIAIVMASQRPDGSWPAAA
                     PASHSRYDTSVTALALLALMKMDGPLPGDYTAAIHDGINHLVRQQDAQGRFTSQFSGV
                     NFTQYLATMAIRAAAARPDAEASWIEASRRADAIPDNSRQMAKLNQNLAHPAAFPPNW
                     LEAGGPVTRAALDLLVR"
     gene            complement(4299..4850)
                     /locus_tag="PKI64_09540"
     CDS             complement(4299..4850)
                     /locus_tag="PKI64_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOE01279.1"
                     /translation="MSAPLQPEPDDFTLMAQVRAGQEDAFRILVERHQRPLLNFFARM
                     GASNHGDDLAQMTFVRLWTYRKKYKPTAKFTTFLYTLARHTWLDFLRRQKRFSLFAER
                     YREEIPASTDGGLGQLQRQLDIQAALEKLSPKLREVLVLAVHQSLAYEEIAKILRIPV
                     GTVKSRVFNAMATLQELFHENRA"
     gene            4990..5295
                     /locus_tag="PKI64_09545"
     CDS             4990..5295
                     /locus_tag="PKI64_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01280.1"
                     /translation="MKKALLFLTVLLGGAMVCLAVGTCKYCGSSSYGSCTLSPHKVHE
                     HINSPGACVYCGSTSYGSCGIAPNKVHKHGSADNKCVYCGSSSFGSCSISPYKVHER"
     gene            5393..6511
                     /locus_tag="PKI64_09550"
     CDS             5393..6511
                     /locus_tag="PKI64_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOE01281.1"
                     /translation="MMDGIPVWGEPIEPNALRQIQRCARTADRAALMADHHKGYAVPI
                     GGVVAYKDKISPSGVGFDIACGNKAVLTDANADDVRRNIPRIMDDIFGHFVFGIGQKN
                     RVRVDHELFDDDAWRLVAVKPLKEMAREQLGTIGGGNHYVDILVDEQERVWIAVHFGS
                     RGLGHKTATWFLEKGGAKDGMDVEPLVIPATSALGEDYLACMHLAGRYAYAGRDWVCA
                     EAARLLGWRIRDEVHNHHNFAWRETHSGEKLWVVRKGATPAFPGQKGFIGGSMGDICV
                     IVEGVAHEHAESSLYSTVHGAGRTMGRMEAKGKYNRRTGQCLRPGKVTQKMMDQRVAA
                     FGTELRGGGLDESPHCYKPLADVLAAHEGTIIESCIYK"
BASE COUNT         1412 a         1680 c         2065 g         1281 t
ORIGIN      
        1 tggtgagacg ggcggggctc gaacccgcga ccacaagctt aaaaggctcg tgctctacca
       61 actgagctac cgtcccatgt tgcgaaacac ggggcgactt ctacgacatt cccccacccc
      121 gcacaagcaa aaaccttttt tcctcacgca gctccgcgga aaaatatgcg cgcaaaacat
      181 caactgcgcc aacgcggagg ttgcggcgga gcgcgtttcg gtgtatcaca cggcacgcat
      241 gacccggctc tatcccaacg acacgattgc cgccatcgcc actgcacccg gcgcgggcgc
      301 cattgccatt gtccgggtgt ctgggccaga tgctttcgcc attgcggacc gtcttttccg
      361 ctgccccgcg ccgccgccct cgcaacgcgc cgcggggaca tttgtccatg gccaggtcgt
      421 gctgccggac accggcgagg tggcggacga tgctctcctg ctggttttcc gtgcgccgta
      481 ctcctacacc ggcgaagatg ccgtggagct gcaagggcat ggcggcaccc tccccgcccg
      541 acgcatactg caggccgtgc tggcttgcgg ggcacgacaa gcggagcccg gcgaattcac
      601 caagcgcgca tttttgaacg ataagttgga tttgactcaa gccgaagccg tgttggacct
      661 cattgccgcc cgctcggacc gcgccgcgcg gctggccacc gcccaattgg acaacgccct
      721 cggccggaca ttgaggaaaa tcagcgattc tgtgctcgcc gccgccgccg atgtggaggc
      781 catgctggat tttcccgatg atgaactccc ccaatcggtt ccgactgaaa tctgctgtca
      841 gttggatgct attatggctc aaatatcggc acttttggcc acttggcacg aggggcactt
      901 ggttcgcgaa ggcgcgcggg ttgtgattgc cggtgcaccg aatgtcggca aatccacgct
      961 cttgaacctg ttgaccgggc gggaacgggc catcgtgacc ccccaccccg gaaccacgcg
     1021 cgacaccatc gaagaaaccc tcgttttaca aggaattccc atccatttga ctgatacagc
     1081 cggattgcgc aacgctccgg agcaaatcga gcaagaaggc atcaggcgca cgcatgctgc
     1141 actggagcag gcggatttgt gcatttatct ggtggatgcc tctcaacccc tccaaaaagg
     1201 cgacgcagac ttccttgcca gccgttcacc acacaaaacg atgattttag ggaacaaaat
     1261 tgacttggga cagcaaattc atgtccaaaa cgtacaaaat cacgaatatt tggccgtttc
     1321 gctcaaaaat gagcccaaaa ttgatgaaat tcaggctaaa atggtcaaaa aaatagtccc
     1381 ggacccggtt ttgggcgact cgacacatgt tgccatctcc acccggcacc aaaagctgtt
     1441 acacaatgca caagaatatg ttacaaatgc aaaagaagcc ctaaaaacgg gttcggaagc
     1501 cgattatttg cccgcggcca acctgttgcg ggacgcgttg gaagaaattg ggcaaatttt
     1561 gggcgaaaat tgcccgccgg acgtgctgga ggccatattt tcaaggtttt gtgtcgggaa
     1621 atgactaaat aatatgttat tataatcaca ataacaaatt attcccctcc cccacccccg
     1681 cgtgactatt ttggttagat tttgacgatg acgtacgacg ttgtagtgat tggcgcaggc
     1741 catgccggct gtgaagctgc tttagccgcc gctcgcttgg gagccacggt ggctttggtc
     1801 accatggatc cagccgcgac cgcccggatg tcgtgcaatc cgtccatcgg cggtatagcc
     1861 aaatcgcaca tggtgtttga gctggatgcc ttgggcggcg aaattgcgct caacacggat
     1921 tgcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggac cttcctgcgc
     2041 ggcaaaatat ttgtcggcaa aaaagtggtc agtggcggtc gtttgaacga gcctgcggaa
     2101 gaccagttga gcctgcaact gcaagatttg ggtttgcggc tggaacggct caaaactggc
     2161 acgccccccc ggctgcatat ccagagcttg gactatagca agatgcagga acagcctggc
     2221 gaggatccag taccgttctt tagctggctt tgccgggaac aggccggaat gttccacgtg
     2281 gaacaatctg cggccaaccc ggctgttcca accgttgttc cacgtggaac attgccgttg
     2341 ccatcattta ccccgtggat ccccgggagc aaccaaattc catgttggct cacacatacc
     2401 aatccgcaga cgattgagat catccgggag catttggccg attccgcgct ctatggcggc
     2461 ctgattgaag gcaccggcgt gcggtattgt ccgtccatcg aggacaaagt cgtcaagttt
     2521 gcggcgcaca acgatcatca cgtttttgtg gaacccgaag gccgggccgt gcccgagatg
     2581 tatcccaacg ggacatccaa cagcctgccg ctggaggtcc aaaaacgaat gatccattct
     2641 atccccggct tggaacaagc ggagttcctg gcaccgggct acgcgattga atatgatttt
     2701 tttgatccac gtcagctctt ccataccttg gaaagcaaga gggtgggggg actctatttg
     2761 gctggccaag tcaacggcac cacggggtac gaagaggctg ccgcccaagg atttatggca
     2821 ggagtgaatg ccgtgatgta tctcactggg cgggaaggga taacgctgaa acgtcacgag
     2881 gcctatattg gagtaatgat tgacgatttg gtgaccaaag gcacgaatga gccttaccgg
     2941 atgtttacct cacgagcgga gcatcggttg ctgctgcggc aggataatgc agtatttcgt
     3001 ctggcccctg tggcggacaa gctgggtatc gtgccgacgg cccgtcggca gatcattcat
     3061 gaacaggcac aggccattgc cgcggaagtc gcccggctga atgaaacgca cgagggcggc
     3121 attacactgg cccaacaatt gaagcgaccg ggagcgtcgt atgccgcgtt gccgggtcgc
     3181 cggcacgatt tggcaccagc cgtcattgag caggtggaat ttgagctgaa gtatcagggc
     3241 tacatcgagc gcgaatggcg gcaaatcgaa aaagcggagc aaatggccaa gcaaaaaatc
     3301 ccggcggact gcgactttca cgccatctcg gcgttgcgct atgaatcgcg cgaaaaactg
     3361 tcggccatcc gccccgtgga tttggggcag gcggggcgta tctctggcat caccccggca
     3421 gatatcgcca ttctatccgt ctggctcaaa aagcaaaacg gctcctgagt tccaggcact
     3481 tccggcggga aggtttcgtc cctcaaccat tcccacccta caaaagccaa acggaacaga
     3541 acggggaggg ggggggcgat tgggacgtag tgatagattg taataaattg cagtacaata
     3601 tattacatta acgaaccagc aagtcgaggg cggcgcgggt gacggggccg ccagcttcca
     3661 gccagttggg agggaaggcg gcggggtgcg ccagattttg gttcagtttg gccatctgtc
     3721 gggaattatc ggggatggcg tcggcgcgtc ggctggcttc gatccatgag gcttcggcat
     3781 cggggcgggc ggcggcggcg cgaatggcca tggtggcgag atattgggtg aaattgacac
     3841 cggagaactg gctggtaaag cgtccttggg catcctgctg gcgaaccaag tggttgatgc
     3901 cgtcgtgaat ggcggcggta tagtcgccgg ggagggggcc atccattttc atgagggcca
     3961 agagggcaag ggcggtgaca ctggtatcgt agcggctgtg gctagcgggg gcggcggcag
     4021 gccaactgcc atcggggcgt tgggaggcca taacaatggc aatcgcagtg ggttgcgtat
     4081 tgagggcggg ggggcgctgg gtgaaccaaa ggcccaagcc caacaggatg gcgatgctgg
     4141 cggcgagtcg cgccacgcgc aggagccacg gcatgtggcg gatttccgct tggcgggcac
     4201 gggcgagggt ttgccgggta aagtcggggg agggggaggg ggggggctgg gcgcgcagag
     4261 tggccagcag gtcggcaaaa cggggattct ggagggggtc aggcgcggtt ttcatgaaac
     4321 aactcctgca gggtggccat ggcgttgaaa acgcgggatt tgacagtgcc gacggggatg
     4381 cgcaaaatct tggcaatctc ttcgtaggca agggattggt gcacagccag cacgaggact
     4441 tcgcgcaatt ttggggaaag tttttccaga gcggcttgga tatcgagctg ccgttgcagt
     4501 tggcccaagc cgccatcggt cgaggcgggg atttcttcgc gatagcgctc ggcaaaaagg
     4561 ctgaagcgct tctgacggcg gagaaaatcc agccacgtgt gccgcgccaa ggtatagagg
     4621 aacgtggtga acttggcggt gggtttgtat tttttgcgat aggtccatag gcgcacaaac
     4681 gtcatttggg cgaggtcgtc gccatggttg gaagcgccca tgcgcgcgaa aaagttcagt
     4741 agggggcgtt ggtggcgctc cacgagaatc cggaaagcgt cctcctgccc ggcgcggact
     4801 tgcgccatga gcgtaaaatc gtcgggctcc ggttggaggg gggcagacat gggcgggcat
     4861 tcttgtcgag ttgaggcgaa aagcaaagtt ttttgcgcag cgacgatgga cggaccggcc
     4921 tggaactggc aggatacccg gcgaagtccg cacgagggaa cggatgcctt atgaccggga
     4981 ggagttgaaa tgaaaaaagc gttgttgttt ttgacggtgc tgctgggcgg agcgatggtt
     5041 tgcctggcgg tgggcacatg caagtattgt gggtcgtcca gctacggcag ttgcacattg
     5101 agcccgcaca aagttcacga gcacatcaac agtccgggcg catgcgtgta ttgtggttcg
     5161 acgagttatg gcagttgcgg cattgcgccg aacaaagtgc acaagcacgg gagcgcggac
     5221 aacaagtgcg tatattgtgg gtcgtccagc tttggaagtt gttcgattag tccgtacaaa
     5281 gtgcacgaga ggtaaacggc ggcgcgccgc gtgacggcag aatgtgaagt ccaaacaaaa
     5341 aaataaaacg aaaatatcgc cgggcgtagt tgccccggcg cgcgcgccgc gcatgatgga
     5401 tggcattccg gtgtggggcg agccgattga gcccaacgcc ctgcggcaaa ttcaacgctg
     5461 tgcgcgcact gcggatcgcg cggcattgat ggctgatcac cacaagggct acgcggtgcc
     5521 cattggcggc gttgtggcgt acaaggacaa gatcagtccg tcgggcgtgg gctttgacat
     5581 tgcctgcggc aacaaggcgg tgctgaccga tgccaacgcg gacgatgtgc ggcgcaatat
     5641 tccgcgcatc atggatgata ttttcggcca ctttgtgttt ggcatcggcc agaagaaccg
     5701 ggtgcgggtg gatcacgagc tgtttgatga cgacgcctgg cggctggtgg ccgtgaaacc
     5761 gttgaaggag atggcccgcg agcagttggg caccatcggt ggcggcaatc attacgtgga
     5821 tattttggtg gatgaacagg agcgggtgtg gattgccgtg cactttggtt cgagaggctt
     5881 aggccacaag acggcgacat ggtttttgga aaagggcggc gccaaggacg ggatggatgt
     5941 tgagccgttg gtgattccgg caacatccgc gctgggcgag gattacctgg cctgcatgca
     6001 cttggcgggg cgctatgcct atgctgggcg cgattgggtc tgcgcggaag cggcgcgcct
     6061 gctgggttgg cgcattcgcg atgaggtgca caaccaccat aattttgcgt ggcgcgagac
     6121 tcacagtggc gaaaaattat gggtggtgcg caaaggcgcc acgccggcct tccccggaca
     6181 aaagggattc atcggcggtt cgatggggga tatctgcgtg attgtggaag gcgtggcgca
     6241 cgagcacgcg gagagttcgc tctattccac cgtgcatggg gccgggcgca caatgggacg
     6301 catggaggcc aagggaaaat ataatcggcg cacgggccaa tgcctgcgac cgggcaaggt
     6361 gacacaaaaa atgatggatc agcgagtggc cgcgttcggc acggaattgc gcggcggcgg
     6421 gctggatgaa tcaccgcatt gctacaaacc gctggcggat gtgttggcgg cgcacgaggg
     6481 aaccattatt gagtcttgca tatataagtg acattatgtc acaaagaaag ctcggctt
//
LOCUS       DAOPNP010000123         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_13394_length_6355_cov_8.749048, whole genome shotgun sequence.
ACCESSION   DAOPNP010000123 DAOPNP010000000
VERSION     DAOPNP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13394_length_6355_cov_8.749048"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1100)
                     /locus_tag="PKI64_09555"
     CDS             complement(147..1100)
                     /locus_tag="PKI64_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOE01282.1"
                     /translation="MIVTTKELFAHAYGKYAIGAYNINNLEQTMGLFKGNVDSKAPFI
                     VQLSKGARSYTHKLMLEAMMKTANEIFPEAVFAIHLDHGDEATCMDCINSGVYSSVMI
                     DASHEPLEKNIEITKRVVEAAHAKGIVVEAELGQLKGVEEDVVAAEHVYTKPEEAEYF
                     VKQTGCDSLAAAIGTSHGAFKFKGDQKLRYDILQEIQKRLPGFPIVMHGSSSVPQDEV
                     ARINAAGGNIKGASGVDASQFLGAAKLGVTKINIDTDGRLVWCRVHREFFRDHPEKFD
                     LRDPGKVFMPEYAKFIGAKNVLLGSAGQLDAAREYILANKK"
     gene            complement(1125..2870)
                     /locus_tag="PKI64_09560"
     CDS             complement(1125..2870)
                     /locus_tag="PKI64_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE01283.1"
                     /translation="MTMMISKFHRLIQSRLLWGIFLVVIVFSFVIWGMVWPSQRDRAG
                     QADAVGTLDGETVGFNEFRAADRSIRLSRALALGRDAASTPETEVLLRQLAWQRVATL
                     REARKLGITASDQELVSVLRANFSTTNGVYDPQRYAAFLQNMVSPLGFSVGLFEQHLR
                     EEIIIQKLANLIGRQALVTPLEIQRTFDTLLDSFTVEYAALKRDDVAANVKVTPDDAR
                     ELFNANPEAFTIPEQRRIQYAAFPIADFTDTESEISEDDIQDYYELHIEDYTTQETDT
                     NGQPRAIVAELSDVRDGIANILRKLNAVDHAEAAANELVFRAIPDRDGTVPDFAVEAE
                     KAGHPAVALAPFAHGDDPVPDGGVAFVLTAFELAPNAYDRVSSPVVGEDNVYILYLDK
                     VLEPRVPEFEEVEERALDAARQKAIGEALMAKGGELRNAITAGLAAGKTLAESAAGFA
                     AEISTTEPFTGLSGASSTNVVEQALVRAVVGYNQGEITEPVLLPDGLLVAHVKQRTSP
                     DEATFDAYQPEISSAIRARRAQGLFHDWQTGLLAPGRFTDLQRRPDSADDEDESDDTD
                     AEQPAPYVNEAELYL"
     gene            3146..6352
                     /locus_tag="PKI64_09565"
     CDS             3146..6352
                     /locus_tag="PKI64_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOE01284.1"
                     /translation="MKLPLTVKMLQDWGGPTTFRDGRSLFERGLVMDATVGEDQRIQG
                     TISWGSRSIKTGARILPDHSCENQCPCRDNVERGVVCSHVIALGLALLARHHDPDRER
                     KRLEEERHAQRLRQAATEFFKRAPSGAPGAVNCAVRLELPEGWREAAPGGQIPVRLGL
                     VMSGAVVPMTQISREITLGLTPQDEAVLFVLEEIVGGALPDELLVGTADFINLLQLHS
                     GRALHAAAGAELPVNATPVQSMLRVDLDHENGELLLTAHTELPFTRGAEIPTYLVAGR
                     AGWVFGGGNFWPLARLLPVPLWALYAQQVVIPRPDVPRFLREEMPGLNGHIRVEAEVK
                     ADVFSFEPETPELRLDVQGSPASLTVKLIAVYGDVELVAGKTAARGSFAHPDPEDILR
                     YTVRNPQVEQEALAVLARYGLTGPAGDALNGVVGSRAVLNFIGSAIPALRRKGWRVLW
                     HGRIQAAVENAVFATPVVSISPERGAESWFEVRFDYDAGEAGGLTSAEIQRALLKGEA
                     FVERGGRTILLDTDAIRALHSVFSDCASGAGRQPGSFRLAPVYAAYAASALQALDGVD
                     VEAPPAWLAAAAQNNRLEPVEGEPLDPMFERVLRPYQKEGVHWLRFLQRNRFGGILAD
                     EMGLGKTLQTLVWLAMATAERASSERKPALLVCPTSLVDNWAAEAARFTPWLKVGTMT
                     GADRHERWEEARNLDILVTSYALLRRDIALHEQMPYFAVVLDEAQHIKNQSTQNAQAA
                     KRLHAEHRLVLTGTPIENGVADLWSIMDFLMPGYLGSRESFRANIEQAIQHGGAEGEL
                     AQWKLRRKLRPFLLRRMKRDVAKELPPKIERVAACALSNDQKKVYTALLENSRQKIVE
                     LVAAQGFLRAKFEILNTLLRLRQACCHLDLLKLPDLQAREPSAKLELFFELLDEALDA
                     GHRVLVFSQFVSMLTILRRELERRKLRYCYLDGATTERLKIVQEFNRDAAIPLFLISL
                     KAGGTGLNLTGADMVIHFDPWWNPAVENQATDRAYRIGQKRTVYSVKLIAQDSIEERV
                     LALQRKKQKLYDATLEDGDDATDIHNLTWEDVQELLSL"
BASE COUNT         1094 a         1818 c         2212 g         1231 t
ORIGIN      
        1 ggccggtgtc cggaaaaatc cccgcggtca ccgcccgcgg ctacagtcaa cagccacgcc
       61 tttgtaggcc ggggttgtat ccccggcggt ccgggctgtt ggtttggcgg tttttacctg
      121 aaccctgaac cccgaaccct gaacccttac ttcttgttgg ccaggatgta ttcgcgggcg
      181 gcgtccagtt ggccggcgct gccgagcagc acgttcttgg cgccaatgaa cttggcgtat
      241 tcgggcatga acactttgcc cggatcgcgc aggtcgaact tttcagggtg gtcgcggaag
      301 aattcgcgat gcacccggca ccaaacgagg cgaccgtcgg tatcaatgtt gatcttggtg
      361 acgccgagtt tggccgcgcc aaggaattgg ctggcatcca cgccggatgc gcccttgatg
      421 ttgccgccgg cggcattgat gcgggcgact tcgtcctgcg gcaccgagga ggagccgtgc
      481 atgacgatcg ggaagccggg caggcgcttc tggatttcct gcaagatatc gtagcgcagt
      541 ttttggtcgc ccttgaactt gaaggcgccg tgcgaggtgc cgatggcggc ggcgaggctg
      601 tcgcaaccgg tttgcttgac gaagtattcg gcttcttccg gcttggtata gacgtgctcg
      661 gcggcgacga cgtcttcctc gacacccttg agctggccga gttcggcttc caccacgatg
      721 cccttggcat gggcggcttc taccacgcgc ttggtgatct cgatgttttt ctcgagcggc
      781 tcgtgggagg cgtcaatcat cacggaggaa tagacgccgg agttgatgca gtccatgcag
      841 gtggcttcgt cgccgtggtc gaggtggatg gcgaagacgg cttcggggaa aatctcgttg
      901 gcggtcttca tcatggcttc gagcatcagc ttgtgggtat agctgcgggc acccttggaa
      961 agctggacga tgaacggggc tttgctgtcc acgttgccct tgaacaggcc catggtctgc
     1021 tcgaggttgt tgatgttgta ggcgccgatg gcgtatttgc cgtaggcgtg ggcaaacagt
     1081 tccttggtgg tgacgatcat ggtggggtct ccttggttga gtttttacag gtatagttcg
     1141 gcctcgttga cgtacggggc cggctgctcg gcgtcggtgt cgtcgctctc gtcttcgtcg
     1201 tcggcggagt cgggccggcg ctgcaaatcg gtgaagcgtc cgggggcgag caggccggtt
     1261 tgccaatcat ggaacaggcc ttgggcgcgc cgcgcgcgga tcgcggagga aatttcgggc
     1321 tggtaggcat cgaaggtggc ctcatcgggt gaggtgcgct gcttgacgtg ggccacgagc
     1381 aggccgtccg ggaggaggac gggctcggtg atttcgcctt ggttatagcc gaccacggcg
     1441 cgcaccagcg cttgttccac cacattggtg gaggaagcgc ccgagaggcc ggtgaacggt
     1501 tcggttgtgc tgatttcggc ggcgaaacca gcggccgact cggccaacgt cttgccggcg
     1561 gcaaggccgg cagtgatggc gttgcgcagc tcgccgccct tggccatgag cgcctcgccg
     1621 atggcttttt ggcgcgcggc gtccaacgcg cgttcttcga cttcctcaaa ctcgggcacg
     1681 cgcggctcca gtaccttgtc gagatagagg atatacacgt tatcctcgcc gacgacgggc
     1741 gagctgacgc ggtcataggc attgggggcc agttcaaacg ccgtcagcac gaacgcaacg
     1801 ccgccatccg gcacagggtc gtccccgtgc gcaaacggcg ccagcgctac ggcggggtgc
     1861 ccggcctttt cggcttcgac ggcgaaatca ggtacggtgc catcgcggtc gggaatggcg
     1921 cggaacacca gctcgttggc ggcggcttcc gcgtggtcaa cggcgttgag tttgcgcagg
     1981 atgttggcaa tgccgtcacg gacatcggac agctcggcca cgatggcgcg gggctggccg
     2041 ttggtgtcgg tttcctgcgt ggtgtagtcc tcaatgtgca gctcgtagta atcttgaatg
     2101 tcgtcttcgc tgatttcgct ctctgtgtcg gtaaaatcgg cgatggggaa agccgcgtat
     2161 tgaatgcggc gttgctcggg gatggtgaac gcctcgggat tggcgttgaa cagctcgcgg
     2221 gcgtcatcgg gcgttacttt cacgttggcg gccacgtcgt cgcgcttgag cgcggcatat
     2281 tccaccgtga aactgtccag cagcgtatcg aaggtgcgct ggatttccag cggcgtcacc
     2341 agcgcctgcc ggccgatcag attggccagt ttctggatga tgatttcctc gcggaggtgc
     2401 tgctcaaaca ggcccaccga aaagcccagc gggctcacca tgttctgcaa aaaggccgcg
     2461 tagcgttgcg ggtcatacac gccgttggtg gtggaaaaat tggcgcgcag cacgctgacc
     2521 agttcctggt cgctggcggt gatgcccagc ttgcgggcct cgcgcaaggt ggccacgcgc
     2581 tgccaggcca gttgccgcag caggacttcg gtctccggcg tggaggcggc atcgcggccc
     2641 aacgccaagg cgcggctcaa gcgaatgctc cggtccgcgg cgcggaattc gttgaagcca
     2701 accgtttcgc catccagcgt gccgacggca tcggcctggc cggcccgatc gcgctgcgaa
     2761 ggccacacca tgccccaaat cacgaacgaa aagacaatga ccaccagaaa tatcccccac
     2821 aacaggcggg actggatcag ccggtggaac tttgaaatca tcatggtcat gctaggactc
     2881 gctttcctca acagataggc aacaccgtcc cgcacggcca aaaaccgggc gggcaacgtc
     2941 ggggcaggag aatacgggcc cccgccatgc cgggcaagaa ccattttcgg cgtaaaagcg
     3001 ccttgccggc atggcggagg ggggggcggg gggcaggggg gggcggcggc agatgagatt
     3061 ttattcttca ttgttgaaac ggggggcggc cacgcggtaa cctgctcctt ttgtctggct
     3121 gtccagaaaa cgccgggagt ggttcgtgaa gttgccgttg accgtcaaga tgctgcaaga
     3181 ctggggtggg cctaccacct tccgggatgg gcgttctttg ttcgagcggg gcctggtgat
     3241 ggatgccacg gtgggggagg atcagcgaat tcaaggcaca atttcgtggg gcagtcgctc
     3301 catcaagacg ggggcgcgga ttctgccgga ccattcctgc gaaaaccagt gtccttgccg
     3361 tgacaacgtg gagcggggcg tggtgtgttc ccatgtgatt gcgctggggc tggcgctgct
     3421 ggcgcggcat catgacccgg accgcgagcg caaacggctg gaggaagaac gccacgcgca
     3481 gcggctgcgg caggccgcca cggagttttt caaacgcgcg cccagcggtg cgcccggcgc
     3541 ggtcaattgc gcggttcgtt tggaattgcc ggagggctgg cgcgaggccg cgcccggcgg
     3601 ccagataccg gtgcggttgg ggttggtaat gtccggcgca gtcgtgccca tgacgcaaat
     3661 ctcgcgcgag atcaccttgg ggctgacgcc gcaggatgaa gcagtgctgt tcgtgttgga
     3721 agagattgtg gggggcgcgc tgccggatga gctgctggtt ggtacggcgg acttcatcaa
     3781 tttgctgcaa ctgcacagcg ggcgcgcgct gcatgcggcg gcgggggcgg agttgccggt
     3841 gaacgccacg ccggtgcaaa gcatgttgcg cgtggatttg gaccacgaaa acggcgagct
     3901 gttgctgacg gcgcacacgg agttgccatt tacgcgcggc gcggaaattc ccacctatct
     3961 cgtcgccggg cgggcagggt gggtgtttgg tggtggcaac ttctggccgc tggcgcgcct
     4021 gttgccggtg ccgttgtggg cgctatatgc ccagcaagtg gtgatccccc ggccggatgt
     4081 gccgcgcttt ttgcgcgagg aaatgccggg cttgaatggg catatccgcg tggaggcgga
     4141 ggtaaaagcc gatgtgttca gctttgagcc cgagacgccg gagctgcgcc tcgatgtgca
     4201 gggcagcccg gccagcctga cggtgaagct gatcgcggtg tatggcgatg tggagctggt
     4261 ggcggggaag acagcggccc gcggttcttt tgcgcatccg gacccggaag atattttgcg
     4321 ctacaccgtg cgcaatccgc aggtggagca ggaagcgttg gccgtgctgg cgcgctacgg
     4381 cctgacgggt cccgcgggcg atgcgctcaa tggcgtggtg ggcagccgcg cggtgctcaa
     4441 cttcattggc agcgccattc ccgcgttgcg gcggaagggg tggcgcgtgt tgtggcacgg
     4501 gcgcattcag gcagcggtgg aaaacgcggt tttcgcaacc ccggtggtga gcatttctcc
     4561 agagcggggc gcggaaagct ggtttgaggt gcgctttgac tatgatgccg gcgaggccgg
     4621 cgggctgacc agcgcggaaa tccagcgcgc gctgctcaag ggggaggcgt ttgtcgagcg
     4681 gggcgggcgg accattctgc tggatacgga tgccatccgc gcgctgcata gcgtgttcag
     4741 cgattgcgcg tccggcgcgg gacgccagcc cggcagcttc cggctggcgc cggtctatgc
     4801 cgcctatgcg gcgagcgcgt tgcaggcgct ggacggggtg gatgtcgaag cgccgccggc
     4861 ctggctggcg gcggcggcgc agaacaaccg cttggagccg gtggagggcg agccgctgga
     4921 ccccatgttc gagcgcgtac tgcgtcccta ccagaaggaa ggcgtgcact ggctgcgctt
     4981 tttgcagcgc aaccggttcg gcggcattct ggccgacgaa atggggctgg gcaaaactct
     5041 gcaaacactg gtgtggctgg ccatggccac cgcagaacgc gccagcagtg aacgcaagcc
     5101 ggcgctgctg gtgtgcccga ccagcctcgt ggacaactgg gcggcggagg cggcgcgctt
     5161 cacgccgtgg ctcaaggtcg gcaccatgac gggcgcggat cggcacgagc gctgggagga
     5221 ggcgcgcaac ctggatattc tggtgacgtc ctacgccctg ctgcggcgcg acatcgcctt
     5281 gcacgagcaa atgccgtatt ttgccgttgt gctcgatgaa gcacagcaca tcaaaaacca
     5341 gtccacccag aatgcgcagg ccgccaagcg gctgcacgcc gaacaccggt tggtgttgac
     5401 cggcacgccc atcgaaaacg gcgtggcgga tttgtggagc atcatggact tcctcatgcc
     5461 gggctatctg ggcagccgcg aatcgttccg cgccaacatc gagcaggcca ttcagcatgg
     5521 tggcgccgag ggcgaactgg cgcaatggaa gctgcggcgc aagctgcgcc cgttcctgct
     5581 gcggcgcatg aagcgcgatg tcgccaagga attgccgccc aagatcgagc gcgtggccgc
     5641 gtgcgccctc agcaacgacc agaaaaaggt gtacaccgcc ttgctggaaa actcgcggca
     5701 aaaaatcgtc gagctggtgg ccgcgcaggg cttcctgcgc gccaagtttg agattctcaa
     5761 taccctgctg cggttgcgcc aggcctgctg ccatttggat ttgttgaagc tgccggattt
     5821 gcaggcccgc gaaccttccg ccaagctgga gctgtttttc gagctgttgg atgaagccct
     5881 ggatgccggc caccgcgtgc tggtgttcag ccagtttgtt tccatgctga ccattttgcg
     5941 ccgggagttg gaacgacgaa aactgcgcta ttgctatctt gacggcgcga cgaccgaacg
     6001 gctgaaaatt gtgcaggagt tcaaccgcga tgccgccatc ccgctgttcc tcatcagcct
     6061 caaagccggc ggcactggtc tgaatctgac cggcgcggac atggtgatcc actttgaccc
     6121 gtggtggaat cctgcggtgg aaaatcaggc caccgaccgc gcctatcgca ttggccaaaa
     6181 acgcacggtg tacagcgtga agctcattgc gcaggattcc attgaggagc gcgtgctggc
     6241 gctccagcgc aaaaaacaga agctctacga cgccacgctg gaagatggcg acgacgccac
     6301 cgacatccat aacctcacgt gggaggatgt ccaggagctg ctttccctgt aaaac
//
LOCUS       DAOPNP010000124         6102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_14145_length_6102_cov_8.182239, whole genome shotgun sequence.
ACCESSION   DAOPNP010000124 DAOPNP010000000
VERSION     DAOPNP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14145_length_6102_cov_8.182239"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PKI64_09570"
     CDS             complement(<1..1234)
                     /locus_tag="PKI64_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01285.1"
                     /translation="MKSQPVAAGTMPAVPYRQAPLVFLLMLVAVLGMTWPWLQTFSNA
                     MLNHWDPPFHAWKLEYVARAILNGHLLPPDGNTNMYYPYSGTLYYEALHWPQALLAAP
                     LFGWLNLNPVLVYHVILVLFWALTGVCTWMLMLALGSTRRSALLGALLFTIIPYRISY
                     MVEFNMQLNFGIPLFFFFMVRYFQRPAIRYACGMALAWWLQAISELYQAVFLLLILPF
                     PALALMANNWHLLKSFRRFWLPGICAAILGGALTWLILRPYLTMLHVHAVNRGLQEIA
                     THVLEPLSFLRPGGRFHYPPPMDARRDEMIVYPTVALILLNCLHVVNDTRRLLRHRVS
                     RWLWLARVVRWLALIAFFLVTFGIYFFGAPAAGGNLYGLLPVIAILFSVLVLVRPTST
                     NPATLFMTGFFSAAVFAFF"
     gene            complement(1231..2736)
                     /locus_tag="PKI64_09575"
     CDS             complement(1231..2736)
                     /locus_tag="PKI64_09575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE01286.1"
                     /translation="MNTHPELAPVSGTFSAQGKRLGVFVISYNAEKLIQETLQRIPDE
                     IWREVEVVYVVDDCSMDETTRAALTYPDPYRKLKVFRNRINRRYGGNQKFGYQYAIDR
                     GLDTVIMLHGDGQYAPEMLPQLFQPLIDGSADIVIGSRMLHRRDALKGGMPKYKFVAN
                     IFLTGIENLLSGLSMSEFHSGYRGYTTQFLKRIPLWENSDEWHFDSHILFQAKQAGAT
                     ITEIPIPTHYGDEVCHVNGISYGLNCIRSALLFRLHRWGLVRLNRYDIAPHSLVDARK
                     LDDPYSSHSLIYRRLRKMKLTHDKVLDLGFGCAGIAAAITQHGAVLDGVDRDPVAIAE
                     SGPLFRRTFQHDINDLDNIPFNEKYDIILAADILQFLVDPADILSRLKKYLVKGGMLV
                     VALPNVVNVQPRLRILLGRFPLHHRGILEEQALHFYTLDSMKQLLVRAGWQVEETDVT
                     NVPLTLLFPFLRARRWRLPLWLCRGLTLMFKGLFAYQGLLFCKNPNEADLL"
     gene            complement(2805..3830)
                     /locus_tag="PKI64_09580"
     CDS             complement(2805..3830)
                     /locus_tag="PKI64_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01287.1"
                     /translation="MKKIALSFLLACCLSGVLLAQPAPPPTFTPEPPEPAALAFVNHY
                     LELLAADNTEQALALNDLRGMRQYLLERRLAELQAHNPELTAAEIEEISSQLQMHDLD
                     PARLRAILLEVMQQGKFTGMTWAIQGFAPAPEGLDGYLVQISARNADHAEKPFLLGIK
                     KLGEQWQVAPEILEELNRQLAPARLPNLPPPTEVETAVDVFWTRWQQGELAEAYDTFA
                     PEYRGKTSLLAFLQQAQSFVDIAGMPTTWEITRCRPLTPDTLGLGVDVRGLTATRPIV
                     MVYRKAPAGWQLYDIQLQMPLPPEGRPPLPAGPAPFRSNLRPDLKPDLSTPAAPLHQA
                     PTGPTAP"
     gene            4171..5412
                     /locus_tag="PKI64_09585"
     CDS             4171..5412
                     /locus_tag="PKI64_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE01288.1"
                     /translation="MSEFLEFQDVSKHYGDVKAVDHVSLTVKQGEFFSLLGPSGCGKT
                     TLLRILAGFEQPDTGRVLLGGEDVTDLPPYERKVNTIFQSYALFPHLSVWDNIAFGLK
                     VAKRPKKEIKEEVAKMLTLIQMEDQAWKKPDQISGGQKQRVAIARALVNKPRVLLLDE
                     PLAALDLKLRQRMLIELDLIHDEVGITFLYVTHDQGEAMSLSDRIAVMKLGKIEQLGT
                     PEEIYEAPRSSFVAAFIGDTNFFDGTVAEPLEKDSVLNGFLAAAVDKDYSRLKIEDFP
                     DLECFNDKQIKEGERVFLSIRPEKMRISRERPATTPHLNIIAGTVEDVIYLGSQTKYW
                     VRTGDYRISIIRQHSRFLLDEKPIRWHEEVWISWHADDGFMLEKYSAQDENLIQLPPE
                     EVGENPPSLNGADLAGSATAQ"
     gene            5409..>6102
                     /locus_tag="PKI64_09590"
     CDS             5409..>6102
                     /locus_tag="PKI64_09590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE01289.1"
                     /translation="MKAQRRVRVFEWLVTLPSLVWLLVLFLIPTVLVFAITFKPATPY
                     GGIGSGWTLETLRTLGNPNYPAIVWRTIWLSVVVTALCIVLATPMGYYMARISPRRRQ
                     MVLLLVILPFWTSFLVRIFAWKVLLHPEGLLKQLLVFLHLCTPDTSLLYNTFAVLLVM
                     VYTELPFAILPIYAASEKFDFRLIEAAKDLGATSFRAFRAVFLPGIRVGLLTAFLMVF
                     IPSLGSYVIPDIV"
BASE COUNT         1344 a         1653 c         1918 g         1187 t
ORIGIN      
        1 aaaaaaaggc gaacaccgct gccgagaaaa atccggtcat gaacaaggtg gccgggttgg
       61 tggaagtggg ccgcaccagc accaaaacag aaaaaagaat ggcgatgacc ggcaacaggc
      121 cgtagagatt gccgccggcg gccggcgcgc caaagaaata gatgccgaag gtcaccaaga
      181 agaatgcaat gagcgccagc cagcggacca cgcgcgccag ccagagccag cgggagaccc
      241 ggtggcgcag cagccggcgg gtgtcgttca ccacgtgcag gcagttcagc aagatcagcg
      301 ccacggtggg atagacgatc atttcgtcgc ggcgggcatc catgggcgga gggtagtgaa
      361 agcggccacc cggccgcagg aaggaaagcg gttccagcac gtgggtggcg atctcctgca
      421 agccgcggtt gacggcatga acatggagca tggtgaggta tggccgcagg atcagccatg
      481 tcagcgcgcc gcccagaatc gccgcgcaga tgcccggcag ccaaaaacgg cggaacgatt
      541 taaggaggtg ccagttgttg gccatgagcg ccagcgccgg gaaaggcaaa atcagaagta
      601 gaaaaacggc ttggtagagt tcactgatgg cttggagcca ccaggcgagg gccatgccgc
      661 aggcatagcg gatggccggt cgctggaaat agcgcaccat aaaaaagaaa aagagcggga
      721 tgccaaaatt caactgcatg ttgaactcga ccatgtagct gatgcggtag gggataatgg
      781 tgaacagcag cgcgcccagc agggccgagc ggcgagtgga gcccaaggcc agcatcagca
      841 tccaagtgca gacaccggtg agcgcccaaa acaacacgag aatcacgtgg tagaccagca
      901 cgggattgag gttcagccaa ccaaacaatg gcgcggccaa caacgcttgc ggccagtgga
      961 gcgcctcata atacagcgtg ccggaatatg ggtagtacat gttggtgttg ccatcgggtg
     1021 gcagcaggtg gccattgagg atggcgcgcg ccacatattc caacttccag gcatggaacg
     1081 gcgggtccca atggtttagc atggcgttgg agaaggtctg gagccagggc caggtcatac
     1141 ccagcacggc caccagcatc aacagaaaca ccaggggcgc ttgtcggtaa ggcaccgccg
     1201 gcatggttcc tgcggcaacc ggttggcttt tcacagtaga tcagcctcgt tggggttttt
     1261 acaaaacagc aggccctgat aggcgaaaag gcctttgaac atcagcgtca ggccgcggca
     1321 cagccagaga ggcaatcgcc agcgtcgggc ccgcaaaaat ggaaacagca acgtgagggg
     1381 cacgttagtg acatcggttt cttccacctg ccagccggcg cgcaccagca gttgtttcat
     1441 gctgtcgagc gtgtaaaaat gcaatgcttg ttcttccaaa ataccgcggt ggtgcagcgg
     1501 gaagcggccc aacagaatgc gcaggcgcgg ttgcacattg accacattgg gcagggcgac
     1561 aaccaacatg ccccccttca ccagatattt tttcagccgg gagagaatgt cggccggatc
     1621 caccaagaac tgcaaaatat cggcggcgag gatgatgtcg tatttctcgt tgaaggggat
     1681 gttgtcgagg tcattgatgt catgctggaa cgtgcggcgg aacagggggc cggattcggc
     1741 gatggcgacg gggtcgcggt ccacgccgtc cagcaccgcg ccgtgctgcg tgatggcggc
     1801 ggcaataccc gcgcagccga agccgaggtc cagcaccttg tcgtgggtca gcttcatctt
     1861 gcgtaggcgg cggtaaataa gcgagtgcga ggagtagggg tcgtccagtt tgcgcgcatc
     1921 cacaagggag tggggggcaa tgtcgtagcg gttgagccgc accaaacccc agcggtgtag
     1981 acgaaagagc agtgcgctgc ggatacaatt caggccataa gagatgccgt tgacatggca
     2041 gacttcgtca ccgtaatgcg tgggaatcgg gatttccgtg atggttgcac cggcttgttt
     2101 ggcttggaac aaaatgtgcg aatcgaaatg ccattcgtcg ctgttttccc agagaggaat
     2161 ccgttttaga aactgagttg tatagccgcg gtaaccggaa tgaaattccg acatggagag
     2221 gccgctgagt aggttctcga taccggtcag aaaaatattg gcgacaaatt tatatttggg
     2281 catgccgccc ttgagcgcgt cgcgacggtg cagcatacgc gagccaatga cgatatccgc
     2341 cgagccgtca atcagcggtt gaaacaactg cggcagcatt tccggcgcat attggccatc
     2401 gccgtgcagc atgatgacgg tatccaagcc gcggtcaatg gcgtactgat agccgaactt
     2461 ctggttgccc ccgtagcgcc ggttgatgcg attgcgaaat accttcaact tgcgatacgg
     2521 atcgggatag gtgagcgccg cgcgggtggt ttcatccatg ctgcagtcgt ccaccacata
     2581 gacaacttcc acctcgcgcc agatttcatc cggaatccgt tgcaaggttt cttggatcag
     2641 tttttcagcg ttgtacgaaa taacaaacac gccgaggcgt tttccctgcg cggaaaacgt
     2701 ccctgacact ggggccagtt caggatgagt attcatttct gatgcacaca ttcccgagac
     2761 ggcaggctga accattcggg tgaaaaaatc aagtgtttcg cggcttatgg agctgttggc
     2821 ccggtgggcg cctgatgcag gggggcggcc ggggtggaca ggtccggctt caggtcgggc
     2881 cgcaggttgg agcggaaggg ggcagggccc gcagggaggg gggggcggcc ttccgggggg
     2941 aggggcattt gcaattggat gtcgtacagt tgccagccgg ctggcgcctt gcggtacacc
     3001 ataacgattg gccgggtggc ggtaagtccc cgcacgtcaa cgcccaagcc gagggtgtcg
     3061 ggtgtcaggg gccggcagcg ggtgatctcc caagtcgtag gcattccggc aatgtctacg
     3121 aagctctgcg cctgctgcaa aaatgccagc agggaggttt tgccacggta ttccggcgca
     3181 aatgtgtcat aggcctcggc cagttctccc tgctgccagc gggtccagaa aacatccacg
     3241 gctgtttcga cttccgtggg tggcggcaga ttggggagcc gggcgggagc aagctgacgg
     3301 ttcagctcct caagaatttc cggcgccact tgccattgtt cacccagttt tttaatgcca
     3361 agtaaaaatg gcttttctgc gtggtcggcg ttgcgcgcgc tgatctggac cagatagcca
     3421 tctaggcctt ccggagccgg tgcaaaaccc tggatggccc acgtcatgcc ggtgaacttt
     3481 ccctgttgca tgacttccag caaaatggcg cgcaggcggg cgggatccag gtcgtgcatt
     3541 tgcagttgcg aggaaatctc ttcaatctcg gcggcagtca actccgggtt gtgcgcttgc
     3601 agctccgcga ggcggcgttc cagcagatat tgccgcatgc cgcgcaggtc attgagcgcc
     3661 agcgcctgct cggtgttgtc ggcagcgagc aattccaaat agtggttgac gaacgcgagc
     3721 gcggccggtt ccggcggctc cggagtgaag gtgggcgggg gggcgggttg ggcaaggagt
     3781 actccgctca agcagcaggc cagcaggaac gacaatgcaa tttttttcat ggcaataccc
     3841 ttcgagttga aagctgccga ctcccgccgg ccaaccgtca tgccgtgaac tatgccgcaa
     3901 ccggcagggc aagtcaaaca tccgccggac agcaaaaacg ccaaaatacc gcgccaaatg
     3961 ccgtatttgg cgggaaaatg gaaatgccgc ggacgagcaa aaaacgggtg cgccaacagt
     4021 cgccatgcgg cgacctaccc gtcgccgggt ggtccccaaa aaagtgtgaa agttttgatt
     4081 gaaatgaagg gaggctgttc cataatgcgc atcgtttgtt tcagaaaaat gacgggctgc
     4141 tggtcttaaa aacggaggac acacagggct gtgagcgagt ttctggagtt ccaggatgtt
     4201 tctaaacatt atggcgatgt caaagcggtt gatcacgttt cgttgaccgt caagcaaggc
     4261 gaattttttt cgcttctcgg tcccagcgga tgtggcaaga ccacgctgct gcgcatcctg
     4321 gcggggtttg agcagccgga taccgggcgc gtgctgctcg gtggcgaaga cgtgacggac
     4381 ttgccgccct atgagcgcaa ggtcaatacc atcttccaaa gctacgcgtt gtttccgcat
     4441 ctctccgtgt gggacaacat tgccttcggt ctcaaagtgg ccaagcgccc caaaaaagaa
     4501 atcaaggaag aggtcgccaa gatgctcacc ttgattcaaa tggaagatca ggcatggaaa
     4561 aagccggatc agatttccgg cggtcaaaaa cagcgcgtgg ccatcgcgcg cgcgctggtg
     4621 aacaagccgc gcgtactgct gctggatgaa ccgctggccg cgctcgattt gaagctgcgc
     4681 cagcggatgt tgatcgaact ggatctcatt cacgacgaag tgggcatcac cttcttgtat
     4741 gtgacgcacg accagggcga agccatgagc ctctccgacc ggatcgccgt gatgaagctg
     4801 ggcaaaatcg agcaactcgg cacgccggaa gaaatctacg aggccccccg ttcgagcttt
     4861 gtggccgcct tcatcggcga caccaacttt tttgacggca ccgtggccga gccgctggaa
     4921 aaagattccg tgctcaacgg cttcctcgcg gcggcggtgg acaaggacta tagccgcctc
     4981 aaaatcgagg acttcccgga tttggagtgc ttcaacgaca agcagatcaa ggaaggcgag
     5041 cgcgtattct tgagcatccg tccggaaaaa atgcgcatct cgcgcgagcg cccggcgacc
     5101 acgccgcacc tcaacatcat tgccggcacg gtcgaggatg tcatctacct cggctcgcaa
     5161 accaagtact gggtgcgcac tggcgattac cggatttcca tcatccgcca gcacagtcgt
     5221 tttctgttgg atgaaaagcc gatccgctgg cacgaagagg tctggatcag ttggcacgcg
     5281 gatgacggct tcatgctgga gaaatacagc gcgcaagatg aaaacctgat tcaactgcca
     5341 ccggaagagg tcggcgaaaa tccacccagc ctcaacggcg cagacttggc ggggagcgcc
     5401 acggcgcaat gaaagcccag cgccgggtca gggtgttcga gtggctggtc acgctgccct
     5461 cgctggtgtg gctgttggtg ctgtttctga ttcccaccgt cctggtgttt gccatcacgt
     5521 tcaagcccgc caccccgtat ggcggcatcg gctccggctg gacgctggaa accctgcgca
     5581 cgttgggcaa tccgaattat ccggccattg tttggcgcac catctggttg agcgtggtgg
     5641 tgaccgcgct gtgtatcgtg ctggccacgc ccatgggcta ttacatggcg cgaatcagcc
     5701 cgcgccgccg gcagatggtc ctgctgctgg taattctgcc gttctggacc agctttctgg
     5761 tgcgtatttt tgcgtggaaa gtgctgctgc atccggaagg cctgctcaaa cagttgctgg
     5821 tgtttctgca cttgtgcacg ccggacacct cgctgctata caacaccttc gcggtgctgc
     5881 tggtgatggt gtataccgaa ctgccgttcg ccatcctgcc gatttatgcg gcttccgaaa
     5941 aattcgattt ccgcctgatc gaggccgcca aggatttggg ggccacgtcg tttcgcgcct
     6001 tccgcgcggt gtttttgccg ggcattcgcg tggggctgct gaccgctttt ctgatggtgt
     6061 tcattccgtc gctggggtcg tacgtcattc cggatatcgt cg
//
LOCUS       DAOPNP010000125         5923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_14695_length_5923_cov_8.194444, whole genome shotgun sequence.
ACCESSION   DAOPNP010000125 DAOPNP010000000
VERSION     DAOPNP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14695_length_5923_cov_8.194444"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKI64_09595"
     CDS             complement(<1..374)
                     /locus_tag="PKI64_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006096379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOE01290.1"
                     /translation="MQGKFVILLLALLAVCILGGCTMFFLDQGPGSGRRVRMEVTAYC
                     PCGKCCSWTLDKNGRPVISAGKSKGKPKAVGITASGKRAHPGTIAADTRYYPMGTVLY
                     VPGYGYGVVEDRGGDIKGRHRL"
     gene            457..1101
                     /locus_tag="PKI64_09600"
     CDS             457..1101
                     /locus_tag="PKI64_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOE01291.1"
                     /translation="MSQNPAIAVQDWALAGEPFRLRAVNIHAADWPRWLPAAAQERAA
                     RFTDPQAAARAGAAEWFKTGWLAHELGLPRVEWQTAEHGKPQLTGAAAGWGFNLSHAG
                     DYVVAALVKDAAIGVDLESMTRPAEIELLARRVFSPTEQELVRAGGREVFFTLWSQKE
                     ALLKALGCGWADGRLVRRTQLTVAAQQVEPVTGLTIWSRRVLGGAYALAVAMAR"
     gene            complement(1028..1819)
                     /locus_tag="PKI64_09605"
     CDS             complement(1028..1819)
                     /locus_tag="PKI64_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOE01292.1"
                     /translation="MLKFPSALPWLLVLAVAAGVWLGRAGVLPRLFGGRKTIRDRLAQ
                     YGGGARARLAPYFAVANLPYPPRRLALVGLKAERQLQVWAAAANGPWVHLRDYPIRGM
                     SGTLGPKLREGDRQVPEGLYRVESLNPNSRYHLALRVNYPNDHDRRRGREDGRTALGS
                     DIMIHGNTCSIGCLAMGNEAAEDLFVLAAETGVENIAVILSPVDFRARDLPADTPLLP
                     PWAGELYAAIRQELHALGGAALNGPSLPPTHTPPPAPAATKSSAP"
     gene            complement(1834..2886)
                     /locus_tag="PKI64_09610"
     CDS             complement(1834..2886)
                     /locus_tag="PKI64_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01293.1"
                     /translation="MKVFAVWSAFSQNREVDPATRKRTINAILISLGIHVVLVVLAVS
                     LTILVIYPKPKMMFEGKKSPSLPARKLEHAIRVKQMQKQTRKPQLLQRLVTEAPSKVS
                     LPEMPQMTTPDVKNLRDTPLMNSRAGSIGGVGSGGGGAGRGLTGGMGYSDTKFFGENI
                     RTRAITICMDISPSMIAKGVTRDVLDESIKMLENMNVGTKFNIVVFVDGGEAFAPQMV
                     YATQENKQKALDWLKQPFDGRRQGNLRGFSGSTPHMAIKMAVEMGADTVFVLSDDPPY
                     LKQGDMNTGVEIPTHMQDIEKFVRGIESSTGRSVRFYPILYKPFENDRGKQAIEYYKG
                     LARATGGKCRVIPRAP"
     gene            complement(3005..3454)
                     /locus_tag="PKI64_09615"
     CDS             complement(3005..3454)
                     /locus_tag="PKI64_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE01294.1"
                     /translation="MKLKHKTEVKAEVDMTPMIDCVFLLLVFFMVSSTFVKKEADISF
                     ALPGTAEQSDSVDIPDEQIIQITENGNVFLNDLEYDAPTSSDMPELIKTLILFKQTAD
                     ANKVPAMITIAPEDNVKQQRVVDVLNACTAAKIGNVTFAVGSEDEDG"
     gene            complement(3454..3918)
                     /locus_tag="PKI64_09620"
     CDS             complement(3454..3918)
                     /locus_tag="PKI64_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE01295.1"
                     /translation="MKLRKPDEPEAVVDMTPMIDCVFQLLIFFMVCATMSKVDQTPEV
                     SLPIAPKAALQEDLRGRGVVNIVPLGTPVGGTATSEDQPFIIASQVTDEPGLVKAISE
                     RLQAEPNLRIYMRVDRNAEFKIVKRAIRACANAGVFDIIFGAFQSESPGGGT"
     gene            complement(3942..4739)
                     /locus_tag="PKI64_09625"
     CDS             complement(3942..4739)
                     /locus_tag="PKI64_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE01296.1"
                     /translation="MKKQFLALLILLAMLAPVAIALAQDAPPPAPPADNTPPPQKKTL
                     WEEWIRPLGWCGWPLAACSIGVVTMIILNSRMVTRKNLLPMAVVIPLRTAAEQRDVGQ
                     MYSLCRATPNLFTKGLLPGLRKINPDDPAASKEAMESAIAESVGREEAQVSFWINFLS
                     LFASISPMIGLLGTVSGMIGAFGKIGLGGMGRPEQLAENIKEALFTTAAGLMIAIPAI
                     FAFFLFRNNLSRIIREAEALYSEIMDALTGSGNLFAEADQQAPGTEG"
     gene            complement(4805..5725)
                     /locus_tag="PKI64_09630"
     CDS             complement(4805..5725)
                     /locus_tag="PKI64_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01297.1"
                     /translation="MWKTAVWLLVGLPLVASAQLGPWPIQTKQAAFEAAIYKREKDIL
                     WVERKTADGSRAPQVGIALADIVQIRMPRPALLDQVEKIRLAPNAPDSAFAQAHTALD
                     KLILQLKPLRDIPGIYANDAMLAKGRLYDAKNLWRESVRIYEDIISKAPGTAVATNAQ
                     ILAGIAYAKGGEYNFAIEYLGGMPLPEEDEELLSKLLFALGDSFYALENYDNALLSYL
                     PLVVFYPYVYNNEPRGLAAALGCYAKLKEWEPLYRTIQEIKASYPGTPAAKAADDILA
                     EYKTELINAGQFVDAAKITSTDGPVAPPSE"
     gene            5727..>5923
                     /locus_tag="PKI64_09635"
     CDS             5727..>5923
                     /locus_tag="PKI64_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01298.1"
                     /translation="MEKSFPHCGKIGARTARFFHAMEKLCGIFPRNGKTLAKFSTQWK
                     NFDGFFHTMENRRVPSPSPKDA"
BASE COUNT         1013 a         1693 c         2084 g         1133 t
ORIGIN      
        1 tcgaggcggt ggcggccctt gatgtcgccg ccgcggtctt cgaccacgcc gtagccgtag
       61 ccggggacgt agagcacggt gcccatgggg tagtagcggg tgtcggcggc gatggtgccg
      121 gggtgcgcgc gcttgccgct ggccgtgatg cccacggcct tgggtttgcc tttgcttttg
      181 ccggcggaaa tgaccggccg cccgtttttg tcgagcgtcc aagagcagca tttgccgcag
      241 gggcaatagg cggtgacttc catgcgcacc cggcgtccgc taccgggacc ctggtccagg
      301 aaaaacatgg tgcagccgcc caagatgcac accgccagca gcgccagcaa caagatgaca
      361 aacttaccct gcatgatggt gcgttcacca tggcctagac gcaggcaatt gacaaataga
      421 tttattcccg gcgggggttt gtggcaggat gcggccatga gtcagaatcc ggccattgcc
      481 gtgcaggatt gggcgttggc gggcgagccg tttcgcctgc gggcggtgaa tatccatgcc
      541 gcggattggc cgcgctggct gccggccgcg gcgcaggagc gcgcggcgcg gttcacggat
      601 ccgcaagccg cggcgcgggc gggggcggcg gagtggttca agacgggttg gctggcgcac
      661 gagttgggcc tgccgcgggt ggagtggcaa acggcggagc atggcaaacc acaactgacc
      721 ggcgcggcgg cgggatgggg tttcaatttg tcgcacgcgg gcgactacgt ggtggcggcg
      781 ctggtgaagg atgcggccat tggcgtggat ttggaatcca tgacccggcc ggcggaaatc
      841 gaactgctgg cacgccgagt tttttcgccc acagagcagg agttggtgcg ggcggggggg
      901 cgcgaggtgt tcttcacgct atggagccag aaagaggcgc tgctcaaggc gctgggctgc
      961 gggtgggcgg atggccgttt ggtgcgccgg acgcagttga cggtggccgc gcaacaggtg
     1021 gagccggtca cggggctgac gatttggtcg cggcgggtgc tggggggggc gtatgcgttg
     1081 gcggtagcga tggcccgttg agggcggcac cacccagcgc gtgcaattcc tgccggatgg
     1141 cggcatacaa ttcacccgcc cacggcggca ggaggggggt gtccgcgggc aagtcgcgcg
     1201 cgcggaaatc caccggcgac aaaatcacgg cgatgttttc caccccggtt tccgcggcga
     1261 ggacgaacaa atcttccgcg gcctcattac ccatggcgag gcagccgatg gagcaggtgt
     1321 tgccgtggat catgatgtcg gagcccagcg cggtccggcc atcttcacgg ccgcgccggc
     1381 ggtcgtggtc gttgggatag ttcacgcgca gggccaggtg gtagcggctg ttggggttca
     1441 gcgattccac gcggtagagg ccctcgggca cttgccgatc accctcgcgc agcttggggc
     1501 ccagcgtgcc gctcatgccg cggatggggt agtcgcgcaa gtgcacccag gggccgttgg
     1561 cggcggcggc ccacacttgc agttggcgct cggccttgag gcccaccagc gcgaggcggc
     1621 gcggcggata cggcagattg gccacggcga aataaggcgc caggcgcgcg cgggcgcccc
     1681 cgccatattg ggcgaggcga tcgcggatgg ttttcctgcc gccgaagagc cgcggcagaa
     1741 cgccggcgcg gcccagccac acgccggccg caaccgccaa aacaagcagc cagggcaggg
     1801 cggaggggaa tttcagcatg gcgccggtgg cgcctacggg gcgcgcggaa tcacgcggca
     1861 tttgccgccg gtggcgcggg cgaggccttt gtaatactca atggcctgtt tgccgcggtc
     1921 gttctcgaag ggcttgtaga ggatgggata aaagcgcacg gagcggccgg tggagctttc
     1981 gatgccgcgc acaaattttt cgatgtcctg catgtgggtg gggatttcca cgccggtgtt
     2041 catgtcgccc tgcttgaggt agggcgggtc atcggagagc acaaagacgg tgtcggcgcc
     2101 catttcgacg gccattttga tggccatgtg cggggtggaa ccggagaagc cgcgcaagtt
     2161 gccctgccgg cggccgtcga acggctgctt gagccaatcg agcgccttct gcttgttttc
     2221 ctgcgtggca tacaccattt gcggcgcgaa ggcctcgccg ccatccacga acaccacgat
     2281 gttgaacttg gtgcccacgt tcatgttttc gagcatcttg atggattcat cgagcacgtc
     2341 gcgggtgacg cccttggcga tcatggaggg tgaaatatcc atgcagatgg tgatggcgcg
     2401 ggtgcggatg ttttcgccga agaacttggt gtcggagtag cccatgccac cggtgaggcc
     2461 gcgcccggcc ccgccgccgc cgctaccgac gccgccgatg gagccggcgc gcgagttcat
     2521 caggggggtg tcgcgcaggt ttttgacatc gggggtggtc atctgcggca tttcgggcag
     2581 ggacaccttg gagggcgctt cggtcacgag ccgctgcagc aactgcggtt tgcgggtttg
     2641 tttttgcatt tgcttgacgc ggatggcgtg ctccagcttg cgcgcgggga gggagggcga
     2701 cttcttgccc tcgaacatca ttttgggctt gggataaatc accagaatgg tcagcgacac
     2761 cgccagcacc accagcacca cgtggatgcc cagcgagata aggatggcgt tgatggtgcg
     2821 cttgcgggtg gcggggtcca cctcgcggtt ttgcgaaaaa gcgctccaaa cggcaaaaac
     2881 tttcatgaca gcctccggcg gaaccattcg gcgggcggcg ggcacccgtc cactccaact
     2941 attccccacg cgctccgtgt tgtccacccg gcgccgcgcc gttccgttga gcgggggcgg
     3001 ggcatcagcc gtcctcgtct tcgctgccga cggcgaacgt cacgttgccg attttggcgg
     3061 cggtgcaggc gttgagcaca tccaccacgc gctgctgctt gacgttgtct tcgggggcaa
     3121 tggtgatcat ggccggcacc ttgttggcat cggcagtttg cttgaagagg atcagcgttt
     3181 tgatcagctc gggcatgtcg gagctggtgg gcgcgtcata ttccagatca ttgaggaata
     3241 cgttgccgtt ctcggtgatc tggatgatct gctcgtcggg gatgtccacg gaatcggact
     3301 gctcggcggt gccgggcagc gcgaaggaga tatcggcctc ctttttgacg aaggtgctcg
     3361 agaccatgaa gaagaccagc agcaggaaga cgcagtcaat catgggcgtc atgtccactt
     3421 cggccttcac ctcggtttta tgcttgagct tcatcaggtg cctcccccgg gcgattcgct
     3481 ttggaaggcg ccgaagatga tgtcgaacac gccggcattg gcgcaggcgc ggatggcgcg
     3541 cttgacgatc ttgaactcgg cgttgcggtc cacgcgcata taaatgcgca ggttgggctc
     3601 ggcctgcagg cgctcgctga tggccttgac caggccgggt tcatcggtga cctggctggc
     3661 gatgatgaat ggctggtctt cggaggtggc cgtgccgccg acgggcgtgc ccagcggcac
     3721 aatgttgacc acgccgcgcc cgcgcaggtc ttcctgcagc gcggccttgg gcgcgatggg
     3781 cagcgagact tccggcgttt ggtccacctt gctcatcgtg gcgcacacca tgaaaaagat
     3841 gagcaactgg aagacgcagt caatcatggg cgtcatgtcc acgacagctt ccggctcgtc
     3901 tggtttgcgc agtttcatgg ggcggctcct cggggcgggg cttatccttc ggttccgggc
     3961 gcctgctggt cggcctcggc gaacaggttg ccgctgccgg tcagcgcgtc catgatttcg
     4021 gaataaagag cttcggcctc gcggatgatg cgcgagaggt tattgcggaa aaggaagaac
     4081 gcaaagatgg ccgggatggc aatcatcagg cccgcggcgg tggtgaagag cgcttccttg
     4141 atgttttcgg ccaactgttc agggcggccc atgccgccga ggccgatctt gccgaacgcg
     4201 ccgatcatgc ccgagacggt gcccagcagc ccgatcatcg gcgagatgct cgcgaagagc
     4261 gacaagaagt tgatccagaa actcacctgt gcttcctcgc gcccgacgga ctcggcgatg
     4321 gcgctttcca tggcctcctt ggaggcggcg gggtcgtcgg gattgatttt acgcaggccg
     4381 ggcagcagcc ccttggtaaa gaggttgggc gtggcgcggc agagcgaata catctggccc
     4441 acgtcgcgct gttcggcggc ggtgcgcagc ggaatcacca cggccatcgg cagcaggttc
     4501 ttgcgcgtga ccatgcgcga gttgaggatg atcatggtca ccacgccgat ggagcacgcc
     4561 gccagcggcc agccgcacca gcccagcggg cgaatccatt cctcccacag cgttttcttt
     4621 tgcggcggcg gggtgttgtc cgccggcggc gcggggggcg gcgcatcctg ggcgagggcg
     4681 atggccactg gcgccagcat ggcgagcaga atcagcaggg cgaggaattg ttttttcatg
     4741 acggtttcct tatggctggg ggtcttgcaa cgacaaacaa aatggaacaa cgggcgcgca
     4801 tgggtcattc gctcggcggg gccacggggc catcggtgct ggtgattttg gccgcgtcca
     4861 cgaactggcc ggcgttgatg agctcggtct tgtactcggc gagaatgtca tccgcggcct
     4921 tggcggcggg ggtgccggga tagctggcct tgatttcctg gatggtgcgg tagagcggct
     4981 cccactcctt gagcttggcg tagcagccga gggcggcggc cagcccgcgc ggctcgttgt
     5041 tgtacacata aggataaaac accaccagcg gcagataaga gagcagggcg ttgtcgtagt
     5101 tttcgagagc gtagaatgag tcgcccagcg caaagaggag tttggaaagc agctcctcgt
     5161 cttcctcggg caggggcatc ccgccgagat attcgatggc aaagttgtat tcgccgccct
     5221 tggcataggc gatgcccgcg aggatttgcg cgttggtggc cacggcggtg ccgggcgcct
     5281 tgctgatgat gtcctcatag atgcgcacgg actcgcgcca gaggttcttg gcgtcgtaga
     5341 ggcggccctt ggcgagcatg gcgtcgttgg cataaatgcc ggggatgtcg cgcagcggct
     5401 tgagctgcaa gatcagtttg tccagcgcgg tgtgggcctg cgcaaacgcg ctgtccggcg
     5461 cgttgggggc caaccggatt ttttccacct gatccagcag cgccgggcgc ggcatgcgga
     5521 tttggacaat atcggccaag gcaatgccca cctgcggcgc gcgcgagcca tcggctgttt
     5581 tacgctccac ccagaggatg tctttttcgc gcttgtaaat ggcggcttca aaagcggcct
     5641 gtttggtctg gatgggccac gggcccaact gcgcggaggc caccagcggc agcccaacca
     5701 gcagccacac cgcggttttc cacactgtgg aaaaaagttt tccacactgt ggaaaaattg
     5761 gcgcgcgcac ggcccgtttt ttccacgcta tggaaaaact ttgcggaatt tttccacgca
     5821 atggaaaaac tttggcgaaa ttttccacac aatggaaaaa ctttgacgga tttttccaca
     5881 caatggaaaa taggcgagtg ccgagcccaa gtccgaagga cgc
//
LOCUS       DAOPNP010000126         5861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_14912_length_5861_cov_8.964175, whole genome shotgun sequence.
ACCESSION   DAOPNP010000126 DAOPNP010000000
VERSION     DAOPNP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14912_length_5861_cov_8.964175"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..832
                     /locus_tag="PKI64_09640"
     CDS             338..832
                     /locus_tag="PKI64_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011789895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE01299.1"
                     /translation="MVIINTNLGEIHVKLAADKAPLTVANFLAYVDDGFYNGTIFHRV
                     IDGFMIQGGGFEPNMRQKSTRAPVKNEAANGLHNKRGTIAMARTPVVDSATAQFFINV
                     KDNGFLDFRSPDPQGFGYCVFGEVVAGMDVVDKIKAVRTGTKAGMTDVPLDTVQILSV
                     TRAP"
     gene            883..1857
                     /locus_tag="PKI64_09645"
     CDS             883..1857
                     /locus_tag="PKI64_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOE01300.1"
                     /translation="MSKRARKAWVGFDLGGTKMMAVVFDQQFKVLATAKAKTPVNEGA
                     KAVLTQIRLTIREALHAAKIAPRALSGIGVGCPGVLDLDTGVILEAPNLAWRRVPLRR
                     LLGRWFRCRVVLANDVDAGTYGEYRFGAGQNARTLLGVFPGTGIGGGCVYEGKLLRGK
                     KGSAMEIGHIPVRENSRLCGCGKRGCLEALAGRLAISAEVAQAAYRGEAPYIATHAGS
                     DLKKMKSGLLAKAIKAGDAVVARILGDAMRLLGRTIGGVVNLLAPDVIVLGGGLVEAM
                     PQLVLKEVKAGVQETAMSSFTRQLNVRIAKLGDNATALGAAALAAEAR"
     gene            1860..>2327
                     /locus_tag="PKI64_09650"
     CDS             1860..>2327
                     /locus_tag="PKI64_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOE01301.1"
                     /translation="MADRDLNSSPVDATATPATAPAPAAPLEMVRRLVAAIDIGSSAL
                     RLDIGEIGTQGSVRLLESLRHGVRLGKDVFTKGRVEQDTMRRCVAIMKNYSRLLKEYG
                     VTRPDQVRAVATSFAREAANRDMFVDRISTATGIAVNVLEESEESRLTFLAVRE"
     assembly_gap    2328..2427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2428..3372
                     /locus_tag="PKI64_09655"
     CDS             <2428..3372
                     /locus_tag="PKI64_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE01302.1"
                     /translation="EKISALVVMGAGARFAVEQGGAVPLATEPRLAVVPATKLAEVAE
                     QVAPLAVESLVRTHKMAFQDAETVAPALMSLTQVARALKVKELHIARATLRDGLLGEM
                     AMREHLAVGYEEQVIYSAQTLAARYGMDAAHAQNVTVGALALFDELQEEHQLGSHARL
                     LLRCAAILHDIGQFVNARAHHKHSLYLIQNSELFGLTRHDMQIVATVSRYHRKALPSP
                     AHPEFAMLSQDDRMTVAKLAAILRVADALERTHREAPRPMEFRREGERFVIVLRNMED
                     LTMERLALKDKGNLFADVFGMIPELREVRTDVPGGVTG"
     gene            3382..5514
                     /gene="ppk1"
                     /locus_tag="PKI64_09660"
     CDS             3382..5514
                     /gene="ppk1"
                     /locus_tag="PKI64_09660"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOE01303.1"
                     /translation="MATKRKKPREESRFLNRELSWIEFNFRVLQHAEDASQPPLERLR
                     FVAITASNADDFFMVRVGGLKMLQQEGREKPDPAGLTPSAQLTLIAERMREMVMRQQA
                     CLTREIEPALRKGGIRRLKFAELSAAQLRHVEAVFEDSIFPVITPVAVAPGEEFPLLA
                     GLSLALYLRLAGTPDDATPRHAIVALPRVLARFLTLPDEAGYSYILLEDVVRAYVDRL
                     FPGVPVLECAAFRITRNADMSVSDDGAGDLLAQMREVLTERRESDCVRLEIEKRASAG
                     AQAFLRTALHLDPADVYPVSGPLDLAAFFRLANLPGFEPLKLDAWNPQPVPEAPPTEL
                     MFDTLKRGDLLLVHPYESYDPVVRLLQEAADDPNVLAIKQVLYRVSENSPVVAALMRA
                     AERGKHVTALVELKARFDEARNIDWADALARAGAQVIYGVKGYKTHAKVCVVVRREPE
                     GVARYTHFGTGNYNEKTARLYADVSFLTRAENYGADASVFFNTITGYSQLAKFRKVEA
                     APLGIRPRLLELINSETERARQGEEAAIEAKMNALVDPELIEALYRASQEGVKIRLNV
                     RGICCLRPGVKGWSENITVISIVDRYLEHARIFCFLNGGNPRLFMASADWMPRNLDRR
                     IELMIPVEDPASADKLTQYLAACLADTRQAWQLQPDGSYVRRQPDPRHPSLRCQEELQ
                     RRAEAAAMAATQRQAPVFEPQRPAEQKK"
     gene            5592..>5861
                     /locus_tag="PKI64_09665"
     CDS             5592..>5861
                     /locus_tag="PKI64_09665"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOE01304.1"
                     /translation="MLAEQRAIVLKNERPALAGSVDALHDLRVALRRIRCLAATFVKL
                     DKKKMTDLDRQLAEVCDAVGLARDLDVWGDLFRRLLRTGGLQQITR"
BASE COUNT         1086 a         1736 c         1864 g         1075 t
ORIGIN      
        1 taaggtgagt gatttttgtg tttttatctg cgcggtgcgg ccccgaacat cagttgccgc
       61 ccccaatacc cccactacaa tcggtgaaga gccgtaaaac cgcatttttt ccgggtcgcg
      121 gcgggctttt catcggcggc caacagtggt aagatgccct tttatgtgag gagatgcgcc
      181 cgtgaaatgg atttatgcaa ttttggctac gacgttgatc ggcggccccg ccgccttggc
      241 ccaggaggag tcggccccgc cgccggtccc tgctttgagt gtggaatcag aacccgcgcc
      301 ggcggaaccg gcaatggaac ccgcaaagga tacaactatg gtcatcatta acacgaactt
      361 gggcgaaatt catgtgaagc tggctgccga caaggcgccg ctgaccgtgg ccaacttttt
      421 ggcgtatgtg gacgatggat tttacaacgg caccatcttc caccgcgtga ttgatggctt
      481 catgatccaa ggcggcggtt ttgagcccaa tatgcggcag aagtccaccc gcgcgccggt
      541 caagaatgaa gccgccaacg gcttgcacaa caagcggggc accatcgcca tggcgcggac
      601 gcccgtggtg gacagcgcga cggcgcagtt tttcatcaac gtgaaggaca acggttttct
      661 cgatttccgc tcgcccgatc cgcagggctt cggctattgc gtgttcggcg aagtggtggc
      721 cggcatggat gtggtggata aaatcaaggc ggtgcggacc ggcaccaagg ccggcatgac
      781 cgatgtgccg ctcgacaccg tccagatttt gtcggtcacg cgggcgccat agcaaatagg
      841 caacaggtgg gcgggagacg tcgcccggag aggacggcga tcatgagcaa gcgcgcaaga
      901 aaagcatggg tgggcttcga tctgggcggc accaaaatga tggcggtggt ctttgatcag
      961 caattcaagg tgctggccac cgccaaggcc aaaacgccgg tcaacgaagg ggccaaggca
     1021 gtcttgacgc aaatccgcct gaccatccgc gaggcgctgc acgcggcgaa gattgcaccg
     1081 cgcgcgctga gcgggattgg cgtgggctgc ccgggcgtgc tggatttgga taccggcgtg
     1141 attctggaag cgcccaatct ggcatggcgg cgtgtgccat tgcgccggtt gctggggcgg
     1201 tggttccgct gccgcgtggt gctggccaac gacgtggatg ccggaaccta cggggaatat
     1261 cgttttggcg ctggccagaa cgcgcggact ttgctgggcg tttttcccgg caccggcatc
     1321 ggcggcgggt gcgtgtacga aggcaaattg ctgcgcggca aaaagggttc cgccatggaa
     1381 attggccaca tccctgtgcg cgaaaacagc cgattgtgcg gctgtggcaa gcgtggctgc
     1441 ctcgaggcgc tggccggccg gctggccatc tccgctgagg tggcccaggc agcgtatcgc
     1501 ggcgaagcgc cctacatcgc gacgcatgca ggcagtgacc tgaaaaaaat gaaaagcggg
     1561 ctgctggcca aagccatcaa ggccggcgat gcagtggtgg cgcgcatttt gggcgatgcc
     1621 atgcgcctgc tgggccgcac catcggcggc gtggtgaact tgctggcgcc cgatgtcatt
     1681 gtgctgggtg gcgggctggt ggaagccatg ccgcaactgg tgctcaagga agtgaaggcc
     1741 ggcgtgcagg aaacagccat gtcgtcgttc acccggcaac tgaatgtgcg gattgccaag
     1801 ctgggcgata acgccacggc cttgggcgcc gcggcgctgg cggcggaggc gcgctgaaca
     1861 tggcggaccg ggatctcaac tccagccccg tagatgccac cgcgacgccg gcaacggccc
     1921 cggcgccggc agcgccgctg gaaatggtgc gccggctggt ggcggcgatt gatatcggct
     1981 cttcggcctt gcgcttggat attggcgaaa tcggcacgca aggaagcgtc cggctgttgg
     2041 aaagcctgcg ccacggcgtg cggctgggca aagatgtgtt caccaagggg cgcgtggaac
     2101 aggacaccat gcgccggtgc gtagccatca tgaaaaatta cagccgcctg ctgaaggaat
     2161 atggcgtgac gcgccctgat caggtccggg cggtggcaac cagcttcgcg cgcgaagcgg
     2221 cgaaccgcga catgtttgtg gaccgcatca gcacggcgac cggcatcgcg gtgaatgtgc
     2281 tggaggaatc cgaagaatcg cgcctgacct ttttggccgt gcgcgagnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa aaaatttccg cgttggtggt gatgggggcc
     2461 ggcgcgcgct tcgccgtgga gcaggggggg gcggtgccgc tggccacgga gccgcggctg
     2521 gccgtggtgc cggccaccaa attggcggaa gtggccgaac aggtcgcgcc cttggccgtg
     2581 gaaagcttgg tacgcacgca taaaatggcg tttcaggacg cggagacggt tgcgccggca
     2641 ttgatgtcgc tgacccaggt ggcccgcgcg ctcaaggtca aagagctgca tattgcgcgg
     2701 gccacgctgc gcgacgggct gctgggcgaa atggccatgc gtgagcactt ggctgttggc
     2761 tatgaggagc aggtgattta ttccgcgcaa actctcgccg cgcgctacgg gatggatgcc
     2821 gcccacgccc agaatgtcac cgtcggcgcg ctcgcgttgt tcgatgaact gcaagaggaa
     2881 caccagcttg gcagtcacgc gcgcttgctg ctgcgttgcg ccgccatctt gcacgatatt
     2941 ggccagttcg tgaacgcccg tgcgcaccac aagcattcgc tgtacctgat ccagaatagc
     3001 gagctgttcg gcctgacccg ccacgacatg caaatcgtgg ccaccgtctc gcgctatcac
     3061 cgcaaggcgc tgcccagccc ggcgcatccc gaatttgcca tgctgtcgca agatgaccgc
     3121 atgacggtcg ccaagctggc cgccattctg cgggtggcgg atgcgctgga acggacgcac
     3181 cgcgaagcgc cccggccgat ggagtttcgc cgcgagggcg aacgcttcgt cattgtgttg
     3241 cgcaatatgg aagatttgac catggagcgc ctcgccttga aggacaaggg caacctgttc
     3301 gccgatgtct tcggcatgat ccccgaactg cgcgaagtgc gcaccgacgt tcccggcggt
     3361 gtcaccgggt gaggatgtag catggccacc aaacgtaaaa agccccgcga ggaatcccgt
     3421 tttcttaatc gagaattgag ttggatcgaa ttcaatttcc gcgtgctgca acatgcggaa
     3481 gatgccagcc agccgccctt ggagcggttg cgttttgtgg ccatcaccgc ttccaacgcg
     3541 gacgactttt tcatggtacg cgtgggcggc ctgaaaatgc tgcagcagga aggccgtgag
     3601 aagccggacc ccgccggctt gacgcccagc gcgcagctta ccttgattgc cgagcgaatg
     3661 cgcgagatgg ttatgcgtca gcaggcctgc ctgacacgcg aaatcgagcc tgccctgcgc
     3721 aaaggaggta ttcgccggct caagttcgcc gagcttagcg ccgcccagtt gcgccatgtg
     3781 gaagctgttt ttgaggactc cattttcccg gtcattactc cggtggccgt tgcgcccggc
     3841 gaagagttcc cgctgctagc cggcttgagc cttgcgctct atttacgctt ggccggcacc
     3901 ccggacgacg ccaccccgcg ccacgcgatc gtggccttgc cgcgggtgct ggcgcgcttt
     3961 ctaaccctgc cggatgaggc gggttattcg tatatcctgc ttgaggatgt cgtgcgcgcg
     4021 tatgtggacc gcctgttccc cggcgtgccg gtgttggaat gcgcggcgtt ccggatcact
     4081 cgcaatgccg acatgagcgt gagcgatgat ggtgccgggg acctccttgc ccagatgcgc
     4141 gaagtcctca ccgaacgtcg cgaaagcgat tgcgtgcggc tggaaattga aaaacgcgcc
     4201 tcggccggcg cgcaggcttt cttgcgcact gcgctgcact tggacccggc cgatgtctat
     4261 cccgtgtccg gccccttgga tttggcggcg ttcttccggt tggccaatct ccccggcttt
     4321 gagccgctga aactggatgc atggaacccg cagccggtgc ccgaggcgcc acccaccgag
     4381 ttgatgttcg atacgctcaa gcgcggcgat ctgctgctgg tgcaccctta cgaaagctat
     4441 gacccggtgg tccgcctcct gcaagaagcc gccgatgacc ccaatgtgct ggccatcaag
     4501 caggtgttgt accgtgtcag cgagaacagc cccgtcgtgg ctgccctgat gcgcgcggcc
     4561 gagcgcggca aacacgtcac cgccctggtt gagttgaagg cccgcttcga tgaagcgcgc
     4621 aacattgatt gggccgatgc gctcgcccgg gccggcgcgc aagtgattta tggcgtcaaa
     4681 ggctataaaa cccacgccaa ggtgtgcgtg gtggttcggc gcgagcccga aggcgtggcg
     4741 cgctacaccc atttcggcac cggcaactat aacgagaaaa ccgcccggct ctatgccgat
     4801 gtcagtttcc tgacccgcgc cgaaaactac ggcgcagatg cctcggtctt tttcaacacc
     4861 atcaccggct attcgcagct tgccaagttt cgcaaagtgg aagccgcgcc gttgggcatc
     4921 cgcccccggc tgctggaact catcaacagc gaaaccgagc gcgcccgcca aggtgaagag
     4981 gcggccatcg aagccaagat gaatgccctg gtggaccccg aattgatcga ggccctctac
     5041 cgggcctcac aggaaggcgt caaaatacgg ctgaatgttc gcggcatctg ctgccttcgc
     5101 cccggcgtga aggggtggag cgaaaatatc accgtcatca gcattgtgga ccgctatctc
     5161 gaacacgccc gcatcttctg tttcctcaat ggcggcaatc cgcggctgtt catggccagc
     5221 gccgactgga tgccccgtaa tctggaccgg cgcatcgaac tgatgattcc cgtggaagac
     5281 cccgccagcg cggacaagct cacgcaatat ttggctgctt gcttggccga tacccgccag
     5341 gcgtggcaat tgcagcccga tggctcctac gtgcgccgcc agccggatcc ccggcacccg
     5401 tccttgcgct gtcaggaaga attgcagcgg cgcgcggaag ccgcagccat ggcggcaacg
     5461 caacgacaag cacctgtgtt tgaaccgcag cgccccgccg aacagaaaaa gtagccgcga
     5521 tggcccggtc atcagtccca gcaaccagtt cgcgacgtgc gtctgaacgt gccgcaacag
     5581 tcttttgccg attgctggcc gaacaacgcg ccattgtgtt gaaaaacgaa cgccccgctc
     5641 tggccggctc ggtggatgcc ttgcacgacc tccgcgtcgc gttgcgccgc attcgctgtt
     5701 tggccgccac attcgtcaag ctggacaaga aaaaaatgac tgatctcgac cggcaactgg
     5761 ccgaagtatg cgatgccgtc ggattggccc gtgacctgga tgtctggggc gatctgtttc
     5821 gccgcctgct acgaaccggc ggcttgcagc agatcacccg t
//
LOCUS       DAOPNP010000127         5773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_15199_length_5773_cov_7.988283, whole genome shotgun sequence.
ACCESSION   DAOPNP010000127 DAOPNP010000000
VERSION     DAOPNP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15199_length_5773_cov_7.988283"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1253)
                     /locus_tag="PKI64_09670"
     CDS             complement(204..1253)
                     /locus_tag="PKI64_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOE01305.1"
                     /translation="MKTNHIGKWFGWVLVAALISMGRVALAQDAPDDVVLEAPAEDDA
                     PPVAEPPVVEPEPAPEPAPGEDAPILAPELPPVVEPGTGDEPMEPIKPPKKTPTYKGA
                     DMQSAFTGAGSSHFGISGRVNKLPAKKSVRKEKGEWKRNVELGINTASGNSELLRYNG
                     ALSAGRETDQHAIFLKAAGRYGESEKEKDTENATAEAKFQRKLTARNYVALDGNARHD
                     QIADLAYRVRANLSLGRHIVADERVVLSAEIGPGYVAEKKGDDREGFAAGRVAQYLEI
                     LMADNWQVWESLEYVQNLEDSAVYFITAEVGLEVVLTGSMTLKFSVENRYDNQPAANK
                     KSNDLLTTTALSWSF"
     gene            complement(1300..2757)
                     /locus_tag="PKI64_09675"
     CDS             complement(1300..2757)
                     /locus_tag="PKI64_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE01306.1"
                     /translation="MKWQFHPVTRQRWARFRGLRRGWWAFWIFVGAFVVSLGAEWLAN
                     DKPLWIRFEGRNYFPVVKFYPEDVFTGNGRMTRPDYRALAASDMFAPGSGNRMVWPLI
                     PFGPLESLTPGDIALPPVVTVTARPVTFVVSADVDATGRILRVQGDAEHFHLAVGAEL
                     PIPDAVRAGIAERLANRAAPAVDASVAGGWISLPPFTPRDETPQRVRVTLRDKLAAGA
                     ARQAVVDEEQPVYPPDFPAPGSAGWQLSTTKEQVRFPFRPVRGHPLGIDSAGRDVAAR
                     MLYALRTSLLFGMLLVLTTMALGTLAGAVQGYYGGKVDLLGQRFIEIWESLPFLYVLI
                     LLGSVFGQSFGLLLAIYAIFNWIGISYYMRAEFLRLRKLPFVEAARVIGLPPRKIIWR
                     HMLPNALAPLVTFFPFLLVGAIGVMAALDYLGFGLPPPTPSWGELLAQAQEFPQAWWL
                     VLYPALALFFTMLAGVFIGEGLRAAFDPKPFGRLE"
     gene            complement(2804..3196)
                     /locus_tag="PKI64_09680"
     CDS             complement(2804..3196)
                     /locus_tag="PKI64_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOE01307.1"
                     /translation="MENLPDKELTEKIIAAAIQVHRELGPGYLESVYEEALCLELEAS
                     GMAFERQKSVEVRYRGQIVGLYRLDLLVESKVVVELKAIKEFEPIHYSVVRSYLKATG
                     LATGLLLNFHSVPLAIKRIGREFKGSES"
     gene            complement(3362..4408)
                     /locus_tag="PKI64_09685"
     CDS             complement(3362..4408)
                     /locus_tag="PKI64_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE01308.1"
                     /translation="MTSMERVDYFLRRVVLMVPTFVGITLVCFALCQVVPGGPVEQAL
                     AKMRGLEAGAAGRGPHAGAAVTAEQRQALEAHFGFDQPILKRYWTWLAHDKMGLAARS
                     YKYPNKTVGQLIVDRFRISLVFGISGFVLTYLICIPLGIAKALRHGSAFDAASSVVVF
                     IGYAIPAFAFGMLLKMLFAGTGEGFWDILPAAGAVSVNYDLLSPWGKFKDALAHFVLP
                     VSCYVIGNFAVLTLLMKNSLLEQISQDYVRTVLAKGGSLRRAVWGHALRNSLIPIATG
                     FGGVLGILFAGSVLIEKVFEIPGMGLLSLDAIVSRDYMVFMGILAVTSVLGLLGRLLS
                     DACYLLIDPRIQFD"
     gene            4705..>5773
                     /locus_tag="PKI64_09690"
     CDS             4705..>5773
                     /locus_tag="PKI64_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01309.1"
                     /translation="MIQQGILWVQGEPGALSTIWQEFPVLTAGIAYRLGYNPAEALQW
                     STVIFGTILVGMTMLLTRRLFDSNAAAWIAGMWATTNPALLNYSVNSMPEIGFAACLV
                     SAYAVLASAIGGQSLKPVPILTGYALLALGIYFKPLDSLAAFALTTGWLFLVHVRRMT
                     TISLFLLLGLLIYVAVVTPHYVLQNQGNKSGNFELANRAAALAKGTKAYDSKYENAPR
                     DAWFVEEFKEYNELGALKWLWRHRQEVSRRYVSNLMLSLRIYGQDLFPQAFRIGNALW
                     LAMLMAILAIRLWSPQWKTFLWLLLAVMIFPLGVSLSYVYSRWLIIYIPLIIIIVSGH
                     LVLSPSLWGRRWKMGCWCILLL"
BASE COUNT         1256 a         1930 c         1392 g         1195 t
ORIGIN      
        1 ttcttgtcgg gcttttgtag ggcgggaatg gttgaggaac gaaaccttcc cgccgcgaaa
       61 ggctggaagg tctgcgacca ttccagccct gcaaccaaaa ccggaatcgg ttttttgggg
      121 gccgggacac cccaaaacga aagcgtccgg ttgctatgca acgagtgaca acgaggtttt
      181 cggggtgcgc ggaaatcaag aggttagaag ctccagctca gcgcggtcgt ggtgagcagg
      241 tcgttgctct tcttgttggc cgccggctgg ttgtcgtagc ggttttccac gctgaacttc
      301 agcgtcatgc tgccggtcag caccacttcc aaccccactt ccgcggtaat aaaatacacc
      361 gccgaatctt ccagattctg gacatattcc agcgactccc acacctgcca gttgtcggcc
      421 atgagaatct ccaagtattg cgccacccgg cccgccgcaa acccctcgcg gtcatcgccc
      481 ttcttttccg cgacataacc cggaccgatt tccgcgctaa ggaccacgcg ctcatcggcg
      541 acaatgtgcc ggcccaatga caggttggcg cgcacccggt acgccaaatc ggcaatctgg
      601 tcatgtcgcg cgttgccatc caacgccaca tagttgcgcg ccgtcagttt ccgctggaac
      661 ttggcttccg ccgtggcgtt ttccgtgtcc ttttcttttt cactctcgcc gtagcgaccg
      721 gcggctttca ggaaaatggc gtgctgatcc gtttcgcgcc ccgcgctcaa cgcgccgtta
      781 tagcgcaaca gctcgctgtt gccgctggct gtgttgatgc ccaattccac gttgcgcttc
      841 cactcgcctt tttctttgcg caccgatttt ttggcgggca gcttgttcac gcgccctgaa
      901 atgccgaagt gcgaactgcc cgcgcccgta aaggcggact gcatatctgc acccttgtag
      961 gtgggagttt ttttcggcgg cttgatcggt tccatcggct cgtcgccggt gccgggctct
     1021 accaccggtg gcaactccgg cgccaaaatc ggcgcgtctt cgcccggcgc aggttccggc
     1081 gcgggttcag gctcgaccac gggcggctcg gccaccggcg gcgcgtcgtc ctccgcaggt
     1141 gcttccaaca ccacgtcatc cggcgcatct tgtgccagag ccacgcgccc catgctgatc
     1201 aaagccgcca ccaaaaccca accgaaccat ttgccgatgt gatttgtttt catggcgcgc
     1261 caccctaccc aagcgccccg ccggcgtcaa ccgacgcgcc tactccagcc ggccgaacgg
     1321 cttggggtca aacgccgccc gcaagccttc gccgataaac acccccgcca gcatggtgaa
     1381 aaacagcgcc aacgccggat atagcaccag ccaccacgcc tgcggaaatt cctgggcctg
     1441 cgccagcagc tcgccccagc tcggcgtagg cggcggcaaa ccaaacccca gataatccag
     1501 cgcggccatt accccgatgg cccccaccag cagaaacggg aaaaacgtca ccagcggcgc
     1561 cagcgcgttc ggcagcatat gccgccaaat aattttgcgc ggcggcaggc cgatcacccg
     1621 cgccgcctcc acaaacggca acttgcgcaa ccgcaaaaat tccgcgcgca tgtagtacga
     1681 aatgccgatc caattgaaga tcgcgtaaat cgccagcagg aggccgaagc tctgcccgaa
     1741 taccgaaccc agcaaaatca gcacatacag gaacggtagc gattcccaga tttcgatgaa
     1801 ccgctgccct agcaaatcaa ctttgccgcc gtaatagccc tgcaccgctc cggccaacgt
     1861 accgagcgcc atcgtcgtca gcaccagtaa catcccgaac aggagcgaag ttcgcagggc
     1921 atataacatc cgcgccgcca cgtcacgccc cgccgaatca atccccagcg gatgcccccg
     1981 caccggccgg aacggaaacc gcacctgttc cttggtcgtg gataattgcc agcccgcgct
     2041 gcccggcgcc gggaaatccg gcggatatac cggctgctct tcatccacaa cggcctgccg
     2101 ggccgcgccc gccgccagtt tgtcacgcag cgtcacgcgt acccgctgcg gcgtttcatc
     2161 gcgcggcgta aatggcggga gcgaaatcca gccgcccgcc accgacgcat ccaccgccgg
     2221 cgctgcccga ttggccaacc gctcggcaat ccccgcccgc accgcatccg gaatcggcaa
     2281 ctctgcgccc actgccaaat ggaaatgctc cgcatcaccc tgcacccgca aaatccgccc
     2341 agtggcgtcc acatccgccg acacaacaaa cgtcaccggc cgcgcggtca ccgtcaccac
     2401 cggcggcagc gcgatgtctc ccggcgtcaa gctttccagt ggaccaaatg gaatcaacgg
     2461 ccaaaccatc cggttgccgc tccccggcgc gaacatatcg ctggccgcca gcgcacgata
     2521 atccggacgc gtcattcgcc cgttccccgt gaacacatcc tccggataaa acttcaccac
     2581 tgggaaatag ttccgccctt cgaagcggat ccacagcggc ttatcattcg ccagccactc
     2641 cgcgccgagg ctcaccacga acgcccccac aaaaatccag aaagcccacc accctcgccg
     2701 cagcccccga aaccgcgccc aacgctgccg cgtcaccgga tggaactgcc acttcattcc
     2761 agaaaagcag gaaaacagga aacccatgaa cacaggaatt ttttcatgat tcggagccct
     2821 taaattcgcg accgatccgc ttgatggcca atggaaccga atgaaaattc aacaacaagc
     2881 cagtggccaa cccggtggcc ttcagatatg accggaccac cgagtaatga atcggttcaa
     2941 attccttaat ggccttcaac tccacaacca ccttggattc caccaacaag tccaggcggt
     3001 aaaggccgac aatctgacca cgataacgaa cttccacact tttctggcgt tcaaaagcca
     3061 ttccgctggc ctccaattcc aagcacaagg cttcctcata aacagattcc aaataacccg
     3121 gtccaagttc acggtgtacc tgtatcgccg cagcaataat cttctccgtg agttctttgt
     3181 ccggtaaatt ttccatgtac ccttccctgt tttcctgggt ttcctgcttt cctgctacgg
     3241 ttctctttgg attttctgcc ttcggttcaa tcatgctggc aaatccatca cgcgcctttt
     3301 cctgttttcc tgggtttctt gctttcctgc tggggtttta atcggctttc atttccttgc
     3361 ttcaatcgaa ttgaatcctt ggatcaatca gcaagtaaca cgcgtcggag agcaaccgcc
     3421 cgagcaagcc caacaccgat gtcacggcca ggatgcccat gaacaccatg tagtcgcggc
     3481 tgacaatggc atccaggctc aacaacccca tgcccgggat ttcaaacacc ttttcaatca
     3541 ataccgaacc cgcaaataaa atacccagca ccccgccaaa acccgtggca atgggaatca
     3601 acgagttgcg cagcgcgtgc ccccacaccg cgcgccgcag actgccgccc ttggccagca
     3661 ccgtgcggac ataatcctgg ctgatctgct ccaacagcga attcttcatc agcagcgtca
     3721 acaccgcgaa attgccaatg acgtagcatg ataccggaag cacaaaatgg gccagcgcat
     3781 ccttgaactt gccccacggc gaaagcaggt cataattcac cgacaccgcc cccgccgccg
     3841 gcaaaatatc ccagaagccc tccccggttc cggcgaacag cattttcaac agcatgccga
     3901 acgcgaacgc cgggatcgcg tagccaatga acaccaccac gctactcgcg gcatcaaacg
     3961 cactcccgtg acgcaacgcc ttggcaatcc ccagcgggat gcaaatcagg tacgtcaaca
     4021 cgaacccgga tatcccgaac accaaggaaa tccggaagcg atccacgatc agttgaccga
     4081 cggttttatt ggggtacttg tacgaccgcg ccgccaaccc cattttatca tgcgccagcc
     4141 atgtccagta tcgcttcagg atcggctgat cgaaaccgaa atgggcctcc aacgcctgcc
     4201 gttgttctgc ggtcacggcc gccccggcat gcggcccgcg ccccgccgcc cccgcttcca
     4261 gccctcgcat cttcgccagt gcctgttcca ccggcccccc cggcaccacc tggcacagcg
     4321 caaaacacac cagcgtaatc ccgacaaacg tcggcaccat caacaccacc cgccgcaaaa
     4381 aataatccac ccgctccata ctggtcatac cgtcttcacc cttataaacc aatcctgccg
     4441 cggcggatgg tgcttgattt tgactccgac catgggcatt ctttgattcg gcttgtcagt
     4501 gtgaatattt cgagtcgtct gccaatgctc actaaatcgc caacgatgtt tagctctatc
     4561 atacaatggg ccacagagga aaggcctctt gaactgcccc gacgcatccg cattgctatt
     4621 ccaatcctca tcttgggtct cgccctcggg ctaagaatcc atttagcggg catagcctat
     4681 cccgcccaat tggattccac ccacatgatc caacagggca ttctgtgggt acaaggcgaa
     4741 ccgggagccc tctcaaccat ttggcaggaa ttccccgttc ttacggccgg catcgcttat
     4801 cggctgggat ataaccccgc tgaggcgctc caatggagca cggtcatctt tggaaccatt
     4861 cttgtcggca tgaccatgtt gttgacgcgg cggctgtttg acagcaatgc cgctgcttgg
     4921 attgccggta tgtgggccac caccaacccc gctttgttga actattcagt caattccatg
     4981 ccggagatag gattcgcagc ctgcctagtg tccgcttatg ccgttctggc ttctgccatt
     5041 ggcggacaaa gcctcaaacc tgtccccatt ctgaccggtt atgccctcct cgccctcggc
     5101 atctatttca aaccgctcga ctcccttgcc gccttcgcac tgacaaccgg atggctattt
     5161 ctagttcatg tcagacggat gacaacaatc tcccttttct tgctgctcgg cctgttgatc
     5221 tacgttgccg ttgtgacgcc acactacgtc ctccaaaatc aggggaacaa atcgggcaat
     5281 ttcgaattag ccaaccgtgc tgccgcattg gccaaaggaa ccaaggccta tgactcaaaa
     5341 tatgaaaatg cgcctcgtga tgcctggttt gtcgaagaat tcaaggaata caatgaattg
     5401 ggtgcactta aatggctctg gcggcatcgc caggaagtca gccgccggta tgtctctaat
     5461 ttgatgttat cgttacgcat atatggccag gatctcttcc cacaggcctt ccggatcggt
     5521 aatgcattat ggctggccat gttgatggcc atcttggcta ttcgattatg gagcccacaa
     5581 tggaaaacat ttctgtggct ccttctggcc gtcatgatct ttccactggg agtaagctta
     5641 agctatgttt atagccgatg gcttatcatt tatattcctt taatcattat tattgtgtct
     5701 ggccatcttg tcctgtcgcc ctctttgtgg gggcggcgct ggaaaatggg gtgctggtgt
     5761 attcttcttc tgg
//
LOCUS       DAOPNP010000128         5759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_15259_length_5759_cov_9.394109, whole genome shotgun sequence.
ACCESSION   DAOPNP010000128 DAOPNP010000000
VERSION     DAOPNP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15259_length_5759_cov_9.394109"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1230
                     /locus_tag="PKI64_09695"
     CDS             391..1230
                     /locus_tag="PKI64_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE01310.1"
                     /translation="MIKNILFGVDGSLAGEVARDYAFHLAARLDARLEAVHVVDSRML
                     EFPLIAPQAGVIGLNPSALNSLQEALRARGEALLQAAAAAAAKAGLPLITTLEFGHPA
                     QVLADVQVRTELVVLGRQGEHSKTAPTITGSTMERFVRRADRPCLVTPMAFEPIAKIL
                     VGVDGSPTAGRALREAIGLANALQVPLVILSVADTENDRRRAEQASLDAHSMARAHDC
                     AAASLTAVGPPATTIPAQARETGCNLIVLGAHGHSWIYEHLIGSVAAHVVSNSQMPVL
                     LVR"
     gene            complement(1253..1711)
                     /locus_tag="PKI64_09700"
     CDS             complement(1253..1711)
                     /locus_tag="PKI64_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOE01311.1"
                     /translation="MIEPAQLTPPNEEYQIFEIRPSWLANFGLLFWGIALLPVVGLGL
                     LLLARAWYLVHARRYRLSSQRLFMRTGLIANRLEEIELFRVKDVILEQGVLDRLLGIG
                     NVQVLSTDDTMPRLVVRGIRNPEQVKEQIRGAYRAARQQAGMRTTEFLMS"
     gene            complement(1708..3063)
                     /locus_tag="PKI64_09705"
     CDS             complement(1708..3063)
                     /locus_tag="PKI64_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOE01312.1"
                     /translation="MRGGHVHVAGVAGVGMSALAQALTWTHARVTGSDRFFDAGSDLP
                     IFAALRAGGVELVKQDGAAIDAHTTAVAYSTAIEDDNPDVVAAHRYGVPLRHRAAVLA
                     ELAHGHTVLAVAGTAGKTTTTGMLGWLLEQLGADPTVINGGALVAWARAGGVGSVRRG
                     APDAPWVIEVDESDRSLLNFHPQWSILTNISQDHFTLAEVMELFRGYAAQVQTGIVCG
                     AGVAAALGAPAARLVEVATPPERDSAGWFVTWRGQQLRVPQPGAHNALNALLAAEQAA
                     QLGHAPGAIAEALTRFAGVQRRLERADANGPVRVVDDYAHNPAKIVAAWQAVAEPGRR
                     VLGVWRPHGYGPLRSMLAALTDAFATACRPTDQLWLLPVFDAGGTANRSIQSCDLAEA
                     LRVRGVNAALPETYDALGAELLAAARPGDTILLMGARDPRLPVFAAEIAARCAARQET
                     P"
     gene            complement(3067..3804)
                     /locus_tag="PKI64_09710"
     CDS             complement(3067..3804)
                     /locus_tag="PKI64_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOE01313.1"
                     /translation="MAGKKTKVVDGFSVVRGLARAQQQGLVPPPGSRRPAEDTSARPP
                     PLMQPKDSPHIVKTVEPTKRTIRCFQCGYEFKLAGTTPAIYCAKCREKIDLGDYVIEG
                     PWHEEINTGGSVIIRPTGRLEDVRIRAGNVILEGAMDDRSAIECNQWLELAGATSPDP
                     RQLTMRNLRIAAGCTMKFKYKLQVHHVELLGTLDADVESTGLVSIRAGGHLRGTVRGA
                     HLQVEEGGGLSARVFIWPQGNPLEAGT"
     gene            complement(3879..4259)
                     /locus_tag="PKI64_09715"
     CDS             complement(3879..4259)
                     /locus_tag="PKI64_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOE01314.1"
                     /translation="MSNGTQESVIGADVEIIGTIKSNGNIRLDGKLEGELQCGGNAIL
                     GKNAQVKGNVTATSVSIEGKISGNILAKDKIEMKATATVNGDIKARRLSVEDGVTFVG
                     RSEVNPNAQPMPAPSAPQPGTPQR"
     gene            4385..4921
                     /locus_tag="PKI64_09720"
     CDS             4385..4921
                     /locus_tag="PKI64_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01315.1"
                     /translation="MNTRALWTGLAGLGLLVVAGLMVPWPRPNPRPALPELPPFAAGE
                     RVALLWDAPELPPDVLALVQRAHAAGAIVRVAGPQESLADFAPARCYRPAPWPEQPTG
                     FHPDQWPHIPRGADTNGLQMLVLTPAELAAKNTAVLAAARRFHATGTDDAAGTREARL
                     LAHARRAELYLLLPPPVP"
     gene            5019..5258
                     /locus_tag="PKI64_09725"
     CDS             5019..5258
                     /locus_tag="PKI64_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOE01316.1"
                     /translation="MQTKLTLRMDEELIHQAKRAADRRGKSVSQMVAEFFATLGERPT
                     RSGAYPPITTSLLGVMEGKTADERTYQRHLREKYL"
     gene            5255..5665
                     /locus_tag="PKI64_09730"
     CDS             5255..5665
                     /locus_tag="PKI64_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE01317.1"
                     /translation="MKVLLDTNVVLDVLLNRQPHVADSAQIFRLVEQGHLTGLLCATT
                     ITTLDYLLTQSLGRADSRPVLTRLLQLFDIAPVTRAVLEGALRSAMPDFEDAVLVYAA
                     RRAGADAVITRNPRDFAKAPCPVFDPRQFLAHGR"
BASE COUNT          959 a         1913 c         1865 g         1022 t
ORIGIN      
        1 gcctacggag gatttcttgc ggattttggc ggggctctca cgccaaaata agcggtggat
       61 tttccgatga tggcgcgttg cggacgaccc gagctggact cgtgtccgcg aggggtcgga
      121 cgcagcgcgc cagcgcggaa aagacaccgg ttggttttgg cgccgccaaa atccacaaga
      181 aatcctcctg actaaaccaa caccgactca aggcccacaa gacaaagcaa caagccaaca
      241 gccccgggct caacgagccc ggctgcagga acagccgaca gccaacagcc aaacagcctc
      301 gcgcaggcgc gaaaaacttt tggtgtgcgg ggtcttgcca acggcggcgg gcggggtcat
      361 catggggcgg atggaagagc gaggtctgtc atgatcaaaa atatactctt cggggtggat
      421 ggatcgctcg ccggtgaagt ggcccgcgac tacgcgttcc atttggcggc gcggctcgat
      481 gcgcggctgg aggccgtgca cgtggtggat tcgcgaatgc tggagttccc gctgattgcc
      541 ccacaggcgg gcgtaattgg tctgaacccc agcgcgctga acagcctgca ggaagcattg
      601 cgcgcgcgcg gcgaggcact gctgcaagcc gcggctgcgg cggcagccaa ggccgggctg
      661 ccattgatta ccacgttgga gttcggccac ccggcgcagg tgctggccga tgtccaagtg
      721 cgcaccgagc tggtggtgct gggccggcag ggcgagcatt ccaaaaccgc gccgactatc
      781 acgggctcca ccatggaacg ctttgtgcgc cgcgccgacc gcccgtgcct cgtcacaccg
      841 atggcctttg agcccatcgc caaaatcctg gtgggcgtgg atggctcgcc aacggccggg
      901 cgcgcgttgc gcgaggccat cggtttggcc aacgcgctcc aagtgccgct ggtgatcttg
      961 agcgtggcgg ataccgaaaa tgaccgccgc cgcgccgagc aggccagcct ggatgcgcac
     1021 agcatggcgc gcgcacacga ctgcgccgcg gcctcgttga cggcagtggg cccgccggcc
     1081 acgaccattc ccgcccaggc gcgcgaaacc ggctgcaacc tgattgtgct gggcgcgcac
     1141 gggcacagtt ggatttatga acacctgatc ggcagcgtgg cggcgcacgt ggtgtccaac
     1201 agccaaatgc cggtgctgct ggtgcgctga gtggcgtgca gccccgcgcg gcctacgaca
     1261 tgagaaactc ggtggtgcgc attcccgcct gctggcgcgc cgcgcgatag gcgccgcgga
     1321 tttgctcctt cacctgttcg ggattacgga tgccgcgcac caccagccgc ggcatggtgt
     1381 catcggtgga cagcacctgc acgttgccga tgcccagcag ccggtccagc acgccctgct
     1441 ccagaatgac atccttcacg cggaacaatt caatttcttc cagccggttg gcgatcagtc
     1501 ccgtgcgcat gaaaagccgc tgcgaggaga gtcggtagcg gcgggcatgc acgagatacc
     1561 acgcgcgagc gagcaatagc agccccaggc cgaccaccgg cagcagggct atcccccaga
     1621 acagcaggcc gaagttcgcc agccacgagg ggcggatttc aaaaatctga tattcctcat
     1681 tcggcggcgt gagctgggcg ggttcgatca tggcgtctcc tggcgagccg cgcagcgcgc
     1741 ggcgatttcg gcggcaaaaa ccggcaggcg cggatcgcgc gcgcccatga gcaggatcgt
     1801 gtcgcccggt cgggcggcgg caagcagctc cgcgcccagc gcgtcatagg tttcgggtag
     1861 cgccgcgttg accccacgca cgcgcagcgc ttccgcaagg tcgcaggatt ggatggagcg
     1921 gttggccgtg ccgccggcat cgaacaccgg caacagccag agttgatcgg tcgggcggca
     1981 cgccgttgcg aaggcatccg tcagcgcggc cagcatggag cgcaaggggc cgtagccgtg
     2041 cggccgccac acgccaagaa cgcgtcggcc gggttcggcc accgcctgcc atgccgcgac
     2101 aattttggcg ggattgtggg cgtagtcgtc caccacgcgc acggggccgt tggcatcggc
     2161 gcgctcgagg cggcgttgca cgccggcgaa ccgcgtgagg gcctcggcga tggctcccgg
     2221 cgcgtggccc aattgcgcgg cttgctccgc ggcgagcagt gcattgagcg cgttatgcgc
     2281 gcccggctgt ggcacgcgta gttgttgccc acgccacgtc acgaaccagc cggcggagtc
     2341 gcgctccggc ggcgtggcca cttccacgag gcgcgcggcc ggtgcgccca gcgccgccgc
     2401 cacccccgcg ccacacacaa tgcccgtttg gacttgcgcc gcgtagccgc ggaatagctc
     2461 cattacttcc gccagcgtaa aatgatcctg cgaaatgttg gtgaggatgc tccactgcgg
     2521 gtgaaagttc agcagcgagc ggtcgctctc gtccacctcg atgacccacg gcgcatctgg
     2581 cgcgccgcgc cgtacgctgc ccacgccgcc ggcgcgcgcc cacgccacca gcgcgccgcc
     2641 attgatgaca gtggggtccg cgccgagttg ctccagcagc cagcccagca ttccggtggt
     2701 ggtggttttg ccggcggtgc ccgccacggc cagcacggtg tggccgtggg ccagttccgc
     2761 cagtaccgcc gcgcggtggc gcagcggcac gccgtaccgg tgcgcggcca cgacatcagg
     2821 gttatcgtct tcgatggccg tgctgtaggc caccgccgtg gtgtgggcgt caatggccgc
     2881 gccatcctgt ttgaccaatt ccacgccgcc ggcacgcagc gcggcaaaaa tgggcaggtc
     2941 ggagccggca tcaaaaaaac ggtcggagcc ggtgacgcgc gcgtgagtcc acgtcagcgc
     3001 ctgggccaga gcgctcatgc ccacccccgc cacgccggcc acatgaacgt gcccgccccg
     3061 catgggtcag gttcctgcct ccagcggatt gccctgcggc caaatgaaga cccgcgcgga
     3121 caaaccgccg ccctcctcca cctgcaagtg tgcgccgcgc accgtgccgc gcaggtgccc
     3181 gccggcacgg atggacacca agccggtgga ttccacatcg gcatccagcg tgccgagcag
     3241 ctccacgtga tgcacttgca gcttgtattt gaacttcatc gtgcagcccg cggcgatgcg
     3301 cagattacgc atggtcagtt gccgcgggtc cggcgacgtg gcgcccgcca gttccagcca
     3361 ttggttgcac tcaatggcgg agcggtcatc catggctcct tcgaggatta cattgccggc
     3421 gcggatgcgt acatcttcca gccgcccagt cggccggata atgacgctgc ctccggtgtt
     3481 gatttcctcg tgccatggcc cttcgatgac gtaatcgccc agatcaattt tctcgcggca
     3541 tttggcgcag tagattgcgg gcgtggtgcc ggccagcttg aattcgtagc cgcattggaa
     3601 gcagcggatc gtgcgcttgg tcggctcgac cgtcttgaca atatgcgggc tgtccttggg
     3661 ctgcatcaat ggcggcggcc gcgccgacgt atcctccgcc ggtcgtcgcg aacccggcgg
     3721 cggcaccagc ccttgctgct gggcgcgcgc caagccacgc acgacggaaa atccgtccac
     3781 tactttggtt ttcttgcccg ccacgtcggc actccagccc gcccgttccg gtcagcgcat
     3841 cacgcgccca ccggccggcc ggacgaagct atgcagaatt agcgctgggg tgtgccgggt
     3901 tgcggggcgc tgggcgcggg catcggctgc gcgttcggat tcacttccga gcggccgacg
     3961 aacgtcacgc catcttccac cgacagccgc cgcgccttga tgtcgccgtt gaccgtcgcg
     4021 gtggccttca tttcgatttt atccttggcc aaaatgttgc cgctgatctt gccttcgatg
     4081 gacaccgacg tggccgtgac gttgcccttc acctgcgcgt tcttgcccaa aatggcgttg
     4141 ccgccgcatt gcagctcgcc ttccagcttg ccgtccaagc ggatgttgcc gttgctcttg
     4201 atggtaccga tgatttcaac atcagcgcca atgaccgatt cctgtgttcc gttgctcatg
     4261 ggatttgtct cctggggttg ttattttttt atcgtgcgca gtatgccgat tccccgcggc
     4321 cgggacaagg ccggacgcga gaaaagccgt ttcattttgc gcggatcatg ccatcctgct
     4381 gccgatgaac acgcgcgcgc tatggaccgg gctggccggg ctgggtttgc tggtggtggc
     4441 ggggctgatg gtgccatggc cgcggcccaa cccgcggccg gcgctaccgg agctgccgcc
     4501 gttcgccgcc ggcgagcggg tggcgctgct gtgggacgca ccggagctgc caccggatgt
     4561 ccttgcgctg gtgcagcggg cgcacgctgc cggtgccatc gtgcgcgtgg cggggccgca
     4621 ggaatccctg gccgatttcg cgcccgcccg ctgctatcgt cccgccccgt ggccggagca
     4681 gcccacaggt ttccaccccg accagtggcc gcacattccc cgcggagccg acaccaacgg
     4741 attgcagatg ctcgtgttga cgcctgctga attggccgcg aaaaacaccg ccgtgctggc
     4801 ggccgcccgc cgcttccacg ccaccggcac ggacgatgcc gccggcaccc gcgaagcccg
     4861 gctcctcgcc cacgcccgcc gcgccgaact ttacctcctc ctgcccccgc ccgtgccgta
     4921 gtcccccctc gaacgaattt ggatttggca aaactttatt tcagcccgcc tggaattagg
     4981 cttgccgatg acgtatagtt atgccaagag tgtacgtcat gcaaaccaaa ctgactctac
     5041 gcatggatga agaattgatc catcaggcca agcgcgccgc ggatcggcgc ggcaaatcgg
     5101 tttcgcaaat ggtggcggaa ttttttgcga cgttggggga gcgccctacg cgttcggggg
     5161 cctatccgcc catcacgacc agtctcttgg gcgtgatgga aggcaagacg gcggatgaac
     5221 gcacctatca gcgccatctg cgcgagaaat atctgtgaag gtgctgctgg ataccaacgt
     5281 cgtgctggac gtgttgttga accgccagcc gcatgttgcc gattccgccc aaatttttcg
     5341 gctggtggaa cagggccatc tgaccggctt gttgtgcgcc accaccatca ctacgctgga
     5401 ttacctgcta acccaatcgc tcggccgcgc cgactcccgc ccggtgctga cgcgcctgct
     5461 gcaactgttc gacattgcgc cggtcacgcg ggccgtgctg gagggcgccc tgcgcagcgc
     5521 gatgcccgat tttgaagatg ccgtgctggt atatgccgcg cggcgggctg gtgctgatgc
     5581 ggtcattacc cgcaacccgc gcgacttcgc caaagccccg tgcccggtct ttgatccgcg
     5641 gcaatttttg gcgcacgggc gctagccccc cccgctttgc caccaaaatt tttgggattt
     5701 tgcgtaaaaa acgtaaaatc cagtggagcg gttgacaatc gggattatgc gggatagtt
//
LOCUS       DAOPNP010000129         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_15470_length_5705_cov_11.627965, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000129 DAOPNP010000000
VERSION     DAOPNP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15470_length_5705_cov_11.627965"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKI64_09735"
     CDS             <1..207
                     /locus_tag="PKI64_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01318.1"
                     /translation="LPHEKRNKSDSRLLSVPPFCGKVESKLFHCVEKYSLIFPHRGTF
                     ATDFFTVWKKVYPAWAGFSIAWKN"
     gene            complement(229..960)
                     /locus_tag="PKI64_09740"
     CDS             complement(229..960)
                     /locus_tag="PKI64_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01319.1"
                     /translation="MRWILLALWAGTSCGCAAVVANRPVGETPLDLSSRTEEWSGTWR
                     SPMGPCTLSVVDATNGVLAMTSTQPKGWWRWSNETQRVYLRSGGEWTYASVDYTEGTN
                     ACFLWGKVANEDGLILWWLPDPDKIRPLVETGTLPGNLEKTIKKKKTEPPSEDEQADA
                     ASGEDFPLAVTDISALKAMPDMLDGELIILGDLKAEHYELLAATSNGVMFLWEAPLVL
                     IKKSARTDLRTLRATQKQWMREQGK"
     gene            1231..2568
                     /locus_tag="PKI64_09745"
     CDS             1231..2568
                     /locus_tag="PKI64_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE01320.1"
                     /translation="MQLHKPEVPPPFVHLLRKLNSQVERILSTAPDSRSMRDYLHWDK
                     LRHLTPPDGLTAEEWWLGLKLQRFGQYRPVALEDNRGQAFRFLVPDIVQERLHEIDCL
                     AGGTLRLPEPVTNPQTRDQYLIRSLMEEAITSSQLEGAVTTREVAKEMIRTGRPARDR
                     SERMILNNYLTMKRIVEIKDQEMTPELVLDLHRRVTEGTLDDPAMSGRFRRPEQKVVV
                     DDLEGEIYHVPPPAEQLTERCRLLCDFANGKTPEFFIHPVVRAILLHFWLAYDHPFVD
                     GNGRTARALFYWAMLHGDFWLFEFISISAILRKAPAKYARSFLYTETDDNDLTYFIVY
                     QAGVIQRAIEELHAYIGRKTEEVRESEARLRALRMFNYRQTELLRHALKHPHQEYEVL
                     SHQQSHGTAYETARTDLLQLHDKGLLDMRKRGKTMIFSPAPDLAARLRKAEAK"
     gene            complement(2682..2758)
                     /locus_tag="PKI64_09750"
     tRNA            complement(2682..2758)
                     /locus_tag="PKI64_09750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2722..2724),aa:Pro,seq:cgg)
     gene            complement(3897..5363)
                     /locus_tag="PKI64_09755"
     CDS             complement(3897..5363)
                     /locus_tag="PKI64_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOE01321.1"
                     /translation="MKQRVDLDFGDRAWRVARVLAAVVAVAFCGGWAATSASGQDVPP
                     TGGEWVLDPVLWTADATTVGIHLRPHGFRPVGESGGLRAMPVRGPVFGLPPLEALATF
                     QDDRLAQLGVVLYSRGDAGDLAEPEFFARKADLATRLSQWVGGPGFPQKPLASGGTTQ
                     EIHVWTKGSLRLDLEWAYTRNARAPDGSVIKFRAEYIRLRCRRYDAAQQARLVGGSAQ
                     TAGPATRKPFLTLYDLRARVKTLPDGAHLVSGVPMVDQGPKGYCVAATVERVLRFFER
                     DFDQHKMAQMADTSAAGGTSIEGMLQALRRMSTALDIRVVELYGAESGTEAYDDPAAR
                     RRLLVPKSLARTIKEYNRVAKHARQPEIADLTVARSASVDDIYNLMDRAILKEARNSR
                     TIDRDNFFRQVQRHVDLGIPLAWSLMVGLTPEEAETVGIRGVAGHMRMIIGYHPTTRD
                     ILYSDSWGPGHEQKRMKLEDAWVGTTGLFAIVPREFRY"
     gene            5482..>5705
                     /locus_tag="PKI64_09760"
     CDS             5482..>5705
                     /locus_tag="PKI64_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01322.1"
                     /translation="MKASTTAKITAIALAVSLAAPLGAMARNRSGDILAGALIGVVAG
                     SILTSAANGSGVYVAPQPVYYCPPPPPPPPP"
BASE COUNT         1143 a         1634 c         1735 g         1193 t
ORIGIN      
        1 ttgccgcatg aaaaacggaa caaatctgat tcccggcttt tgtcggtgcc accattctgt
       61 ggaaaagtgg agtcaaagtt gttccattgc gtggaaaaat atagcctaat ttttccacac
      121 cgtggaactt ttgccactga ttttttcaca gtgtggaaaa aagtttatcc cgcgtgggcg
      181 ggattttcca tagcgtggaa aaactaatag cagatcacgt tggccaagct acttgccctg
      241 ctcgcgcatc cattgcttct gggttgcgcg gagggtgcgc aggtcggtac gggccgattt
      301 tttgatcagc acaagcgggg cttcccataa gaacatcacg ccgttggatg tcgccgcgag
      361 caactcataa tgctccgcct ttaagtcgcc caggatgatt agttcaccgt ccagcatatc
      421 gggcatggct ttcagcgcgg atatgtcggt tacggccagt ggaaaatcct cgccggatgc
      481 cgcatcggcc tgttcgtctt cgctcggtgg ctccgttttc tttttcttaa tcgttttctc
      541 cagattaccc ggcagcgtcc cggtctccac caatggtctg attttatccg gatcgggcag
      601 ccaccataat attaggccgt cttcattggc gaccttgccc cacaggaagc aggcgttggt
      661 gccttcggta taatccacgc tggcataggt ccactcgccg ccggaccgca gatagacacg
      721 ctgtgtttcg ttgctccaac gccaccagcc tttcggctgg gtgctggtca tagccagcac
      781 gccgttggtg gcgtccacaa cggaaagcgt gcaagggccc atcggcgacc gccaggtgcc
      841 cgaccattct tctgtccggc tgctgagatc gagcggggtt tcgcccacgg ggcggttggc
      901 tacgacggcg gcacagccac aagaagtgcc agcccaaagg gccaataaga tccatctcat
      961 attacatctc ctggccaaga ggtccggttg cgccgtctgc atgggggcat aacttaacaa
     1021 cgggatcgtt ttcctacaaa cgcctcaaat atgccagaga aattctcgaa gccggcagga
     1081 tggcgccgcc gcagcggcca agagagtggg aaaagcaaag tggccggggc gactctagag
     1141 gaatgccatt ttcataccaa taaacgccaa attagtagtt gtaccggata aactaatgta
     1201 gtacaaatta gtacgaaagt aggagcaacg atgcaactgc ataaaccaga agtacctccg
     1261 ccgttcgtcc atttgctgcg caagctgaat tcgcaggtgg agcggattct atccacggct
     1321 cccgacagcc ggtccatgcg ggattatctg cattgggaca agctgcgcca tttgactccg
     1381 cccgacggtt tgaccgccga ggaatggtgg ctggggctga agttgcagcg gttcgggcag
     1441 tatcggccgg tggcgcttga agataaccgg gggcaggcat tccgtttcct ggtgccggat
     1501 attgtgcagg agcggctgca tgagattgac tgtctggcgg gtggcacgct gcggttgccg
     1561 gaaccggtca cgaatcccca gacgcgcgac cagtacctga ttcgttcctt gatggaggaa
     1621 gccatcacct ccagccagtt ggagggggcg gtaacaacgc gggaggtggc gaaggaaatg
     1681 atccggaccg gtcggcctgc ccgtgacaga agcgagcgga tgatcttaaa caactacctc
     1741 acgatgaaac gaatcgtgga aatcaaagat caagagatga cgccagagct ggtgctggat
     1801 ttacatcggc gggtgaccga aggcacgttg gacgatccgg ccatgtccgg acggtttcgc
     1861 cggcccgagc agaaagtcgt ggtggatgat cttgaaggcg agatttatca cgtccccccc
     1921 ccggcggaac agttgacgga gcgttgccgg ttgttgtgcg acttcgccaa cggcaagacg
     1981 cccgaattct ttattcaccc ggtggtgcgg gcgatcctgc tgcatttttg gctggcctat
     2041 gaccatccgt tcgtggacgg caacgggcgg acggcgcggg cgttgttcta ttgggccatg
     2101 ctgcatggcg acttctggct gttcgagttc atctccattt ccgccatcct gcggaaagct
     2161 ccggcaaaat atgcccggtc gttcctctat acggagacgg acgacaacga tttgacttat
     2221 ttcattgttt atcaggcggg cgtcatccaa cgggcgatcg aagagctaca tgcttatatc
     2281 gggcgcaaga ccgaagaggt acgggaatcg gaggcccggt tgcgggcact gcgaatgttc
     2341 aactatcggc aaaccgagct gcttcggcat gccttgaagc atccgcatca ggagtatgaa
     2401 gtgctgagcc accagcagag ccatggcacg gcttatgaaa ccgctcggac ggatctgttg
     2461 caactgcatg acaagggatt gctggacatg cgcaagcgcg gcaagaccat gatcttttcc
     2521 ccggcgcccg acttggcggc gcggttgcgt aaagccgagg ccaagtgatg ccagcccatt
     2581 tcaccctgtt tacggggtag tgaggggtgc ggtgttgggg tgcctgataa aaataaacgc
     2641 cgctattctt aaagaataac cgcgtttttg actatccaag gtggtcgggg cgactggatt
     2701 tgaaccagcg acttcttggt cccgaaccaa gcgctctacc aggctgagcc acgccccggt
     2761 tttggccgcc atggcggcaa aacagggggg actatagacg cttcaaggcg cggcgcaagc
     2821 ctttttcgaa ccgttgcttt tttccagtga cgctggcccg ccaccggtac aacggcggcc
     2881 gacaagcatc tctcgccaag acgccaagag aagacggtga caagttacag gttgaaggtt
     2941 gaaggagaac agtcgtaaca gccgagcagg aaatcaggaa acccaggaaa gcaggaaaaa
     3001 tcatttcagc atctctcgcc aagacgccaa gaacgccaag gggaagatgg tggcaagtta
     3061 caagttgcag gttgcaggtt gaaggagaac agccgtaaca gccgagcagg aaagcaagaa
     3121 acccaggaaa gcaggaaaaa tcattttctg gttcttatag aggtatggcc tccgtgctgt
     3181 ctaggctggc ggtttgacgg tttctacctg acctctgaac cccgaaccct gaccactcct
     3241 accgcggtca ccgcccgcgg ctacagtcaa cagccaacaa agaaccgcat cattcgtcgt
     3301 tgcgaacttt tgcggcggtt gcgtcgtttg cgttgaaggg tttgcggttt gaaatctcgc
     3361 cgccggtaca acggcggcct acagcgaagc acgaagaacc cagcaccttt ccctggttta
     3421 ttcccctttg cgcgctttgc gtctttgcga gattcttttc ccctcctgtt ttcctgggtt
     3481 tcctgatttc ctgcttggtt ttttgatcct accgcggtca ccgcccgcgg ctacagaaac
     3541 agccaacaaa gaaccgcatt attcgtcgtt gcgaactttt gcggcggttg cgtcgtttgc
     3601 gttgaagggt ttgcggtttg aaatctcgcc gccggtacaa cggcggccta cagcgaagca
     3661 cgaagaaccc agcacctttc cctggtttat tcccctttgc gcgctttgcg tctttgcgag
     3721 attcttttcc cctcctgttt tcctgggttt cctgatttcc tgcttggttt tttgatccta
     3781 ccgcggtcac cgcccgcggc tacagaaaca gccatggcag cccccaaaaa acgcttgcga
     3841 tcttgtgcgg cggttgcgtc gtttgcgttg aaagatttgc ggatttccgc tttaggctaa
     3901 taacggaatt cgcgggggac gatggcgaag agaccggtgg tgccgaccca ggcatcttcg
     3961 agcttcatgc gtttttgttc gtggccgggg ccccaactgt cgctgtacag gatgtcgcgg
     4021 gtggtgggat ggtagccgat gatcatgcgc atgtggccgg ccacgccgcg gataccgacg
     4081 gtttcggctt cctcgggggt caggccgacc atcaggctcc acgcaagggg gatgccgagg
     4141 tccacatggc gctgcacctg ccggaaaaag ttatcgcgat caatggtgcg gctgttgcgg
     4201 gcttccttga ggatggcgcg gtccatgaga ttataaatgt catccacgga agcgctgcgg
     4261 gcgacggtga gatcggcaat ttcgggttgg cgcgcgtgtt tggccacgcg gttgtattcc
     4321 ttgatggtgc gcgcgaggct tttgggaacg agcaggcgac ggcgggcagc ggggtcgtcg
     4381 taggcctcgg tgccgctttc ggcgccatag agttcgacca cgcggatatc cagcgcggtg
     4441 gacatccggc gtagggcttg cagcatgccc tcgatgctgg tgccgccggc ggcgctggtg
     4501 tcggccatct gggccatttt atgctgatcg aagtcgcgtt caaaaaagcg caggacgcgt
     4561 tcgacggtgg cggccacgca gtagcctttg gggccttggt ccaccatggg cacaccgctg
     4621 acgaggtggg cgccatcggg gagcgttttc acccgcgcgc gcaggtcgta gagcgtcagg
     4681 aaaggcttgc gggtggcggg gccggcggtt tgggcgctgc cgccaaccaa gcgcgcctgt
     4741 tgcgcggcat catagcgccg gcaccgcagg cggatatatt ccgcgcggaa cttgatgacg
     4801 ctgccatcgg gggcgcgcgc gttgcgggtg taggcccatt ccaggtccag gcgcaggctc
     4861 cccttggtcc agacatggat ttcctgcgtg gtgccgccgc tggccagcgg tttttgagga
     4921 aagcccggac cgcccaccca ttggcttagc cgggtggcca agtccgcctt gcgggcaaaa
     4981 aactcaggct cggcgaggtc gccggcatcg ccgcggctat acagcaccac gcccaactgg
     5041 gcgaggcggt catcttggaa cgtggccagc gcttcgagtg gtggcaggcc aaacaccggg
     5101 ccgcggacgg gcatggcgcg cagtccgccg gactcgccca cgggccggaa gccgtgcggc
     5161 cgcaggtgga tgccgacggt ggtggcatcc gccgtccaca gtacggggtc cagcacccac
     5221 tccccgccgg ttggcggcac atcctgcccg cttgccgacg tggcggccca cccgccacaa
     5281 aatgccaccg caacgaccgc cgccaacacc cgcgccacgc gccacgcgcg gtcgccaaaa
     5341 tccaaatcaa cccgctgttt catgatgcgg tgaataatcc gctggcgccg caaaagcaag
     5401 ccccggatgg cgtggcgggc ctgtaacctt ggcggaggcg ccggcgacta tgtgaagtga
     5461 aagccaatcc aggagaaagc catgaaagcc agcactaccg ccaaaatcac cgccatcgcc
     5521 cttgcggtca gccttgccgc gccgctgggg gcaatggccc gcaaccgctc gggagatatt
     5581 ctggccggcg ccttgatcgg cgtggtggcc ggctcgattc taacctccgc cgctaacggg
     5641 agcggtgtgt atgttgcccc gcagccggtg tattattgcc cgcccccgcc cccgcccccc
     5701 ccccc
//
LOCUS       DAOPNP010000130         5682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_15558_length_5682_cov_4.237604, whole genome shotgun sequence.
ACCESSION   DAOPNP010000130 DAOPNP010000000
VERSION     DAOPNP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15558_length_5682_cov_4.237604"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1040)
                     /gene="cas7c"
                     /locus_tag="PKI64_09765"
     CDS             complement(129..1040)
                     /gene="cas7c"
                     /locus_tag="PKI64_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOE01323.1"
                     /translation="MSNPIQNRYDFLLVFDVQDGNPNGDPDAGNLPRIDAETGRGLVT
                     DVCLKRKVRNYVQQTQGEKQGYDIFVKEKAILNDSISGAYDKLGINLAKAPEDEKDGK
                     KRIKEGQGQGSEVDKARAQMCQDYYDIRTFGAVMSTGANAGQVRGPVQLTFARSVDPV
                     VTLEHSITRMAVATAAEAEKQGGDNRTMGRKNTIPYGLYVARGFVSAHLAAQTGFNAD
                     DLDLLWKALNNMFEHDRSAARGLMTTRKLIVFKHDNALGQASAQSLFDLVAITRKESS
                     KPPRAFDDYQLEIDRAKLPNGVKIIEQ"
     gene            complement(1058..2821)
                     /gene="cas8c"
                     /locus_tag="PKI64_09770"
     CDS             complement(1058..2821)
                     /gene="cas8c"
                     /locus_tag="PKI64_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOE01324.1"
                     /translation="MILHALKEYYDRKAADPESGIAPPGWENKEIRFVITLNKQGELI
                     LLEDTEEIIERKKRAKRFRIPQSVKRSSGIAANLLWDNPEYVFGVALPKRQEKSTDPD
                     QAVKQKERTQAQHKAFLQRIDDLHLGTDEGIGAVLAFLKNEQTVPILVSRFPDIWKEV
                     LESGGNITFRLQGDDALVCNRPVVQRSIALSLDSAAKDIRCLITGEEDSLAETHAAIK
                     GVWGAQSTGANIVSYNAAAFCSFNKKQGANAPIGRAAEFAYTTALNALLDKDSLQRIQ
                     VGEASTVFWSDKQTDFVDQFAVLFAEPAKDDPDRHTRAVRTLLEAPQSGVLPASDQSD
                     VTFFVLGLSPNASRLAVRFWHRAPLAEMKGKIRQHFQDLQIVHGPKDRLVLSLFRLLT
                     TTATQGKSENIPPNLEGDMMRTILEGLPYPDSILQAAIRRIKAEREIPPIRAALIKAC
                     INRRARFGKHKPQEELTVSLDPTNPSIGYRLGRLFATLEKIQEEANPGITATIRERYY
                     AAASSSPVTVFGRLMTLKNHHLAKLDSPGRRINLEKVIGEIMAGVRDFPAHLPLDEQG
                     RFAIGYYHQRQDFFTKKEKQD"
     gene            complement(2818..3534)
                     /gene="cas5c"
                     /locus_tag="PKI64_09775"
     CDS             complement(2818..3534)
                     /gene="cas5c"
                     /locus_tag="PKI64_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOE01325.1"
                     /translation="MSRGFCLEVSGEYACFTRPEMKVERVSYDVITPSAARAIFDAIL
                     WKPAIRWRIEKIEVLAPIRWANIRRNEVSAVASPRGREIFIEDDRQQRAAMLLVDVKY
                     RLYGVFDFIPPAKRQQVHNPVPEWLVDSEEKTALVSADTRADETEAKYAAMFERRARK
                     GQHFHQPYLGCREFAAHVRLVDPQNEPAVPIPKDDDLGWMLYDMNYSDPHNIQPMFYR
                     PMMKQGVILVPHPDSEEVRK"
     gene            complement(3550..>5682)
                     /gene="cas3"
                     /locus_tag="PKI64_09780"
     CDS             complement(3550..>5682)
                     /gene="cas3"
                     /locus_tag="PKI64_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOE01326.1"
                     /translation="QPLSQHLQNVAKRAADNAAHFQSADWAYLAGLLHDLGKASDAFQ
                     AYLLRSNGLDDSEYDSAGQPSTHSGAGAKWALEKYGPLTGRILAYIITGHHAGIPDWV
                     GGAAPGGALINRLEDAAQELQAVRLWAEQFHLPPESPKPPFKMAEREIHLWVRMLYSC
                     MVDADFLDTEHFMNPASFKERRTFPKLQDLIPGFFRGLDAMQAQAVKSEVNSIRAEVR
                     DACENAASHPPGFFSLSVPTGGGKTLSGTAFALRHAACHGQRRIIYVIPYTSIIEQTA
                     DVLRHHLGVENVVEHHSNLDPETQAKAARLASENWDAPIIVTTSVQFFESLLGARSSR
                     CRKLHNIANSVVILDEVQLLPPELLEPCTDLLRQLVERYGVTIVLSTATQPSLPNIEN
                     VQEIIEASARLPERLRRVVYELPNVLENRSNSWPEVAKELEAYAQVLCVVNTRRDCLE
                     LFRCLPPGTVHLSASMCGAHRSQVIESIKAKLRSGAPIRVVSTQLVEAGVDIDFPVVY
                     RALAGLDSIVQSAGRCNREGRQAAGGKVVVFIPPRSAPKGILRKAEDAARVMLASPIS
                     LEGSEIYPRYFREFYSRVEGRFPYQTLLVQNARSLEFQFREAAAQFHIIDQAHHSVFV
                     RHGEGDALIETLRRQIRDDMPYGWLLRKLQRFSITLPQGQLNTLLHNGQIEELTTGLF
                     VWNGRYDEITGADIFNEDGILSAKDTVL"
BASE COUNT         1277 a         1537 c         1479 g         1389 t
ORIGIN      
        1 cggtaaccat gcgaaagccc aatgggcgaa gacgacatcg tttggctcct ttcctacaaa
       61 gccacctgtc ggccagcgcc ggtttataag aggcaaagca accggcgccg gtcggctttt
      121 tgttcagcct attgttctat aattttaact ccgttgggca acttggcgcg atcaatctcg
      181 agctgatagt catcgaaagc tcgtggcggc ttgctgctct ctttgcgggt aatggccacc
      241 aaatcgaaca gcgactgggc agaggcttgc cccaaagcat tatcgtgttt gaagacaatc
      301 aacttgcggg tggtcatcaa gccccgcgcc gcggagcggt catgctcaaa catattgttg
      361 agtgctttcc acagcaagtc aaggtcatca gcattgaatc cggtttgcgc ggccaaatgt
      421 gccgacacaa acccccgggc aacgtaaagc ccatagggga tggtattttt ccttcccatc
      481 gtccgattgt cgccaccttg cttttcggct tccgccgccg tcgcaacagc catccgcgta
      541 atggaatgct ccaaggtcac caccgggtcc acagatcggg caaatgtcag ttgtacgggt
      601 ccgcgcacct gaccggcgtt ggcgccggtt gacatcaccg caccaaaggt gcggatatca
      661 taataatctt ggcacatctg ggcgcgggct ttgtccactt cagaaccctg cccctgtccc
      721 tcttttattc gcttcttccc atctttttca tcctcgggag ctttggccaa attgattccc
      781 aatttgtcgt aagcgccgct gatgctgtcg ttgagaatcg ctttttcttt gacgaagata
      841 tcataaccct gtttttcgcc ttgggtttgc tggacgtagt tgcggacttt gcgtttcagg
      901 caaacatcag tgaccagtcc gcggccggtc tccgcatcaa tgcggggcag gttgccagca
      961 tcgggatcgc cgttggggtt gccgtcttgt acatcaaaga caagaaggaa gtcataacga
     1021 ttttggattg gattgctcat gattgtattc cttggggtta gtcttgtttt tctttttttg
     1081 taaaaaagtc ctgtcgctgg tggtaatagc cgatggcaaa ccgaccttgc tcatccaacg
     1141 gcaaatgcgc ggggaagtcc cggactcccg ccatgatttc accaattact ttttccagat
     1201 tgatgcgcct tccggggcta tccaatttgg ccaagtggtg gtttttcaac gtcatcaacc
     1261 gcccaaaaac tgtgacgggc gagctggacg cggcagcgta atagcgttcg cggatggtgg
     1321 cagtgatgcc agggttggct tcctcctgga ttttctccag agtggcaaac aatcgcccca
     1381 gacggtagcc aatgctgggg ttagtggggt ctagactcac ggtgagttcc tcctgtggtt
     1441 tatgttttcc aaatcgggcg cgacggttga tgcaggcctt gatgagggcc gcacgtattg
     1501 gcggtatttc tcgttctgcc ttgatccgtc taatggcggc ctgcaggatg gagtccgggt
     1561 aaggcaagcc ttccaagatg gtacgcatca tgtccccttc caaattcggg ggaatgtttt
     1621 cgcttttgcc ctgcgtggcc gtagtagtca acaagcggaa aagtgacaac accagtctat
     1681 ctttagggcc atggactatc tgcaaatcct gaaaatgctg ccgaatcttc cccttcattt
     1741 ctgccagcgg ggcgcgatgc caaaaacgaa ccgccagacg ggacgcattg ggggacaacc
     1801 ccagcacaaa aaaagtgaca tctgattggt cacttgctgg gagaactccg gattgcggtg
     1861 cctctaataa agtccgcacg gcgcgagtat gacggtcggg atcatctttg gccggctcgg
     1921 caaacagcac tgcaaattga tccacaaagt cggtttgctt atccgaccaa aacactgttg
     1981 aggcttcccc aacctgtatt cgttggaggg agtctttatc caacagagcg ttgagggcgg
     2041 tggtataggc gaattcagcc gcacgaccga tgggtgcatt cgcgccttgc ttcttgttga
     2101 acgagcaaaa cgccgcagca ttataggaca caatattcgc accggttgat tgtgcgcccc
     2161 agactccctt gatggcggca tgggtctccg ccaacgaatc ttcttcgccg gttatcaggc
     2221 aacgaatatc cttcgcagca gaatcaagac tcaaggcaat ggatcgttgc accaccggac
     2281 gattacaaac aagcgcgtca tctccctgca gacgaaaagt gatatttcct ccgctttcca
     2341 acacttcttt ccatatgtca gggaagcgag ataccagtat tggaaccgtt tgttcgtttt
     2401 tcaaaaacgc caaaactgcc ccaatcccct cgtccgttcc cagatgcaag tcatcaattc
     2461 tttgcaaaaa tgccttgtgt tgtgcttgag tccgttcttt ttgttttacc gcttggtccg
     2521 gatccgtgga cttctcttgt cgtttcggca aggcaacacc aaacacatac tctggattat
     2581 cccagagaag attggccgct atccccgatg agcgttttac cgactgggga atgcggaacc
     2641 gtttagcccg ttttttacgc tcgataattt cttcggtgtc ttccagcaaa atcaattcgc
     2701 cctgtttgtt cagcgtaatg acgaagcgaa tctccttgtt ttcccacccg ggcggcgcaa
     2761 tgccggattc ggggtcggcc gccttgcggt cataatattc cttgagtgcg tgcagaatca
     2821 ttttctcacc tcttcgctat ccggatgggg caccagaatg accccttgtt tcatcattgg
     2881 ccggtaaaac atgggttgaa tattgtgggg atcggaataa ttcatgtcat atagcatcca
     2941 gccgaggtca tcatctttgg gaatgggtac ggccggttcg ttctgggggt ccaccagtcg
     3001 cacatgggcg gcgaactcac gacaacccag atagggctgg tgaaaatgct gccccttgcg
     3061 cgcccgccgc tcaaacatcg cggcatattt ggcttctgtt tcatccgcgc gggtatcggc
     3121 cgagacgagt gcggttttct cttctgaatc caccagccac tccggtaccg gattgtgcac
     3181 ctgctgacgt ttggcggggg ggatgaaatc aaatacccca taaagacggt atttcacatc
     3241 cacaagcagc atggctgctc gctgctggcg atcatcctca ataaatatct ctctgccgcg
     3301 aggcgaggca actgcagaaa cctcattgcg tcgaatattg gcccagcgaa ttggcgcgag
     3361 cacctcgatt ttttcaattc gccaccgaat ggccggcttc cacaaaatgg catcgaaaat
     3421 ggcgcgggcc gcggacggag taatgacatc gtagctcacg cgttcaactt tcatttccgg
     3481 ccgtgtgaaa caggcgtact cacccgatac ttccaaacag aatcctctac tcatacctct
     3541 tccctttcct cataatacgg tgtcttttgc ggataaaatg ccgtcttcat taaaaatatc
     3601 ggcgccggtt atttcatcgt aacggccgtt ccaaacgaaa agtccggtcg tcagttcttc
     3661 gatttggccg ttatgcaata acgtgttcaa ttgcccttgg ggcaaggtaa tggagaagcg
     3721 ttgcaattta cgcaggagcc agccgtatgg catatcatcg cgaatttgcc gacgcagggt
     3781 ctcaatcaat gcatccccct caccatggcg aacaaatacc gaatggtgcg cctgatcaat
     3841 gatatggaat tgcgcggccg cttctctgaa ctgaaattcc agagatcgag cgttttggac
     3901 cagcagggtt tggtagggaa agcgcccctc tacacgggaa taaaactcgc ggaaataacg
     3961 tggataaatc tcggaacctt ccaaggagat ggggctcgca agcatgacac gcgcggcatc
     4021 ttctgctttg cgtaatattc ctttgggcgc cgatcggggc gggataaaga ccaccacttt
     4081 gccgcctgcc gcctgccggc cttcgcgatt gcacctgccg gcggattgga caatcgaatc
     4141 tagaccagcc agcgcccgat atacgaccgg aaagtcaata tctacccccg cttccactaa
     4201 ctgggtgctg acaactcgaa ttggtgctcc cgaccgcaat ttcgctttga tggattcaat
     4261 cacttgcgac cggtgcgccc cgcacatgct ggctgataag tgaaccgtgc ccggcggcag
     4321 gcaacgaaaa agttcgaggc aatcgcgccg ggtattcacc acgcaaagca cttgcgcata
     4381 cgcctcaagt tccttggcca cttccggcca cgaattggag cggttttcca gaacattcgg
     4441 caactcgtac accacccgac gaaggcgttc cggcagacga gcggatgcct ctatgatttc
     4501 ttgcacgttt tcaatattcg gcaggctggg ttgagtggct gtactgagta cgatggtgac
     4561 accatatcgt tcgacaagct ggcgcagcag atccgtgcaa ggttccaata attcgggtgg
     4621 caacagttgc acttcatcca gaatcaccac gctgttggcg atgttgtgca actttcgaca
     4681 gcggctggac cgggcgccaa gcaaagactc aaagaactgg acactggtgg tgacgatgat
     4741 cggggcatcc caattttcgg aagccaaacg ggccgccttg gcttgtgtct ccggatcaag
     4801 attggaatgg tgttccacca cgttctccac ccccaagtgg tgtcgtaaca catcggccgt
     4861 ctgctcaatg atggaggtgt agggaattac atagatgatc cggcgttggc cgtggcatgc
     4921 cgcatgacga agagcaaacg cagtgccgga aagagttttg ccgccgccgg taggcacgga
     4981 caatgagaag aatccggggg ggtggctcgc ggcgttttca caagcatccc ggacttccgc
     5041 ccggattgaa ttgacttcgg actttaccgc ttgggcttgc atggcatcca agcccctaaa
     5101 gaatcccggg atcagatcct gcagttttgg gaatgttcgg cgctctttga aagaagccgg
     5161 attcatgaag tgttccgtat ccaaaaaatc ggcgtccacc atacaggagt acaacattcg
     5221 gacccacaaa tggatttcac gttcagccat tttgaatgga ggcttcggag attcaggcgg
     5281 caaatgaaat tgttctgccc ataggcggac agcctgcaat tcctgtgcag catcttccaa
     5341 acggttgatc aacgcaccgc cgggcgcggc cccgcccacc caatccggga taccagcatg
     5401 atgcccggtg ataatgtacg ctaaaatcct gccggtgagt ggcccatact tttcaagggc
     5461 ccattttgca cctgctccgg agtgtgttga gggctgcccg gcagaatcat attcagagtc
     5521 atccagtcca ttactcctta agagataggc ttgaaaagca tcactggctt tgccgagatc
     5581 atgcagcaag cctgctaaat acgcccaatc agccgattga aaatgagccg cattgtctgc
     5641 ggctcttttt gcaacatttt gtaagtgctg ggaaaggggt tg
//
LOCUS       DAOPNP010000131         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_15961_length_5570_cov_9.477788, whole genome shotgun sequence.
ACCESSION   DAOPNP010000131 DAOPNP010000000
VERSION     DAOPNP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15961_length_5570_cov_9.477788"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1392)
                     /locus_tag="PKI64_09785"
     CDS             complement(223..1392)
                     /locus_tag="PKI64_09785"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOE01327.1"
                     /translation="MNIGIDLISFHAPAYYLDLRVLAEKRGVDPDKFVVGIGQERMAV
                     APPDQDIVTLAANAALPIVEKAGRENIEMVLFATESSIDQSKAAACFCHRLLELPARC
                     RCLETKEACYGATASLRMAAAMVAQRPQSKVLVLAADIARYDLSSPGEPTQGAGAVAM
                     LVSAHPRVLALDPHSGFYTEDIMDFWRPNYREEALVDGKYSTRMYLTTLIEAWNDYQA
                     QSGRGLADHAHYCFHLPFTKIAYKSLERLHKAAGVPLPPKEEQHRLVDYALRYNRLTG
                     NTYAACLYEALTALLDHAPTPLDGQRLGLFSYGSGCMAEFFSGVVQRGYRQHLFTEQL
                     RAELDSRVALTYRQYEDIFNYGVPRDGADHVFAPYRGGPFRLAGIKDHKRRYEPK"
     gene            complement(1444..2754)
                     /gene="ssnA"
                     /locus_tag="PKI64_09790"
     CDS             complement(1444..2754)
                     /gene="ssnA"
                     /locus_tag="PKI64_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOE01328.1"
                     /translation="MALVIRNALVLTNDDAQPVIPHGEILIEGSKIAAVGAKVEAPAG
                     AEVLDAGGMVAMPGLINAHHHLYSSFARGFAPPGEPASNFVQILERMWWKLDLALDAE
                     AVRYSSYVALLEAIHTGCTTIIDHHASPSCTDGSLDVMEETFRAAGLSGCLCYETSDR
                     NIPADGVAENERFIKKTRAGDGQITALFGLHAQMTLGDATLEATARICAETGAGVHIH
                     VAEDLSDETDCLAKHGMRIIPRLHKFGLTGAKSLFIHGIHLDEAEMDLIAETGTMMVH
                     NPESNMNNAVGTQKMLTLLKKGVLLGLGTDGMTSHMISAARVAHLLQRATLGDPRVAF
                     VEACDMLLNKNAQIAARCFPEPRGQLKPGQLADVAIFDYVPTTPLEPGRVLGHLLYGL
                     NYARCHTTIARGKVLMRDSKVLTLDEREICAKAHECARQTWARF"
     gene            complement(2905..3507)
                     /locus_tag="PKI64_09795"
     CDS             complement(2905..3507)
                     /locus_tag="PKI64_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOE01329.1"
                     /translation="MKNAVPIILFGAWVVESVVLAVGPVSRATWWAENITVWVVVTPV
                     VWLYVKGIRFSNTAYVLMSVLVFLHTIGGHYTFAEVPFGWVTDWLGAQRNHFDRLAHF
                     TVGFWIFPILEFVEGRGLSRNRAFSLVFGLAAILAVAALFEMVEWLYAVLADPTAGTA
                     FLGSQGDVWDAQKDMLADGLGAVAAGALYHMLRPQMPNAV"
     gene            complement(3777..4439)
                     /locus_tag="PKI64_09800"
     CDS             complement(3777..4439)
                     /locus_tag="PKI64_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01330.1"
                     /translation="MNIQKTVKVAFVVALVASMGLLSACAVAEDSGHSSHDASSAVRS
                     AAGNKLHDTMRELWAQHMEWTYAAVTAHGTPAFEATAARLMRNQEDIGGAVALFYGKE
                     AGDAVTKLLKEHIAGAVKIVEVAKAGNDEEGLNNAIKAAYDNAQEIADFLSAANEHWP
                     QAFVRDMLKSHIDTTLVYATAILQGKYAEGIEAYGAAEAHMMMLADALSDGLIAAFPE
                     KF"
     gene            4589..>5570
                     /locus_tag="PKI64_09805"
     CDS             4589..>5570
                     /locus_tag="PKI64_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOE01331.1"
                     /translation="METIDGKLVALDARWVFRELSGIGRYTLELLRQLGDLGGGWRFL
                     VLVRDAERREFVEAAAGLGGRGNMEFAEVPFGPFALRGQWSVARLLRARGVAVYHSTN
                     FMIPLPAFSRREPRAIRAVCNIHDLIPMVHPEFTPRALKTRFFPVYRALMHEIARRVD
                     RVLTGSESAKRDIQRLLGVAESRIRVAADGVDARYVPGGEKPSARGGPKTILYVGRSD
                     PYKNLPGLVAVVARLIEEGLPVRLRIAGPPDARYPAAAELAQRLNVAAHVEWLGYLDE
                     AGLLRAYQEADVLALLSRYEGFGLPVLEAMACGTPVVCSNVASLPEVAGDA"
BASE COUNT         1043 a         1713 c         1703 g         1111 t
ORIGIN      
        1 attcataatg cacgcactct atcactagtc acctacttat gcaagtctca atagcctctc
       61 ccggcgggaa ggtttcgttc ctcaaccatt cccgccctac aaaagccctg caaaatcagc
      121 gaaattattg ggtgtcccat tgcggaagcc aggaaagatt tttttcggca gcgccggctg
      181 gagaacaagc agggggatgt agcggacgat gccgagtagg cgctacttcg gctcgtagcg
      241 gcgcttgtga tccttgatgc ccgcgaggcg gaatggcccg ccacgatacg gtgcgaagac
      301 gtggtcggcg ccatcgcgcg gcacgccata attgaagatg tcctcatact ggcggtaggt
      361 cagcgccacg cggctgtcca attcagcgcg gagttgttcg gtgaacaaat gctgccggta
      421 gccccgctgc accacgccgg agaaaaattc cgccatgcag ccagagccgt aggagaagag
      481 gccgaggcgc tggccatcca acggcgtcgg cgcgtgatcc agcagcgcgg tcagcgcttc
      541 gtagaggcac gccgcgtagg tgttgccggt cagccggttg tagcgcagcg cgtaatccac
      601 caagcggtgt tgctcctcct tgggcggtag cggtacgcct gccgccttgt ggaggcgctc
      661 caatgatttg taggcaattt tggtgaacgg cagatggaaa caatagtgcg cgtgatcggc
      721 caagccgcgg cccgactgcg cctggtaatc gttccaggct tcgatcagcg tggtcagata
      781 catccgcgtg gaatacttgc catccaccag cgcttcctcg cggtagttcg gccgccagaa
      841 atccatgatg tcttcggtgt agaagccgct gtgcgggtcc aaggccagca cgcgcggatg
      901 ggcgctgacc agcatggcca ccgcgcccgc gccctgcgtg ggctcgccgg ggctggagag
      961 atcgtaacgc gcgatgtccg ccgccagtac caggaccttg ctttgcgggc gctgcgccac
     1021 catggccgcc gccatgcgca gcgaagccgt cgcgccgtag cacgcttcct tggtttcgag
     1081 gcagcggcag cgcgccggca actccagcag ccgatggcaa aaacacgccg ccgccttgga
     1141 ttggtcaatg ctcgattcgg tggcaaacag caccatttcg atgttttcgc gcccggcttt
     1201 ttccacaatc ggcagcgctg cgttggccgc cagcgtcacg atgtcctggt ccggcggtgc
     1261 caccgccatg cgctcttgcc cgatgcccac cacgaacttg tccgggtcca ccccgcgctt
     1321 ttccgccagc acgcgcaaat ccagataata cgcgggcgcg tggaaggaga tcagatcaat
     1381 gccgatgttc atgttggcca ccgtgggttg tgtgcgcggg aagattccgc cggctgatcc
     1441 cgcttaaaac cgcgcccagg tttgccgcgc gcactcgtgt gccttggcgc agatttcgcg
     1501 ctcgtcgagc gtcagcacct tggaatcgcg catgagcacc ttgccgcggg cgatggtggt
     1561 gtggcagcgt gcgtagttca ggccatagag caggtggccc agcacgcggc cgggttcaag
     1621 cggggtggtt ggcacatagt caaaaatggc cacgtccgcc aactggcccg gcttgagctg
     1681 gccgcgcggt tcggggaagc aacgcgccgc aatctgcgcg ttcttgttga gcagcatgtc
     1741 gcacgcttcc acaaacgcca cgcgtgggtc gcctagcgtg gcgcgctgca gcaggtgcgc
     1801 cacccgcgcc gccgaaatca tgtgcgacgt cattccatct gtgcccagcc ccagcagcac
     1861 gcccttcttc agcagcgtca gcatcttttg cgtgcccacc gcgttgttca tgttcgattc
     1921 cggattatgc accatcatcg tgcccgtctc cgcaatcagg tccatctccg cctcatccag
     1981 atgaatcccg tggatgaaca gcgacttcgc ccccgtcagt ccgaacttgt gcaaccgcgg
     2041 aataatccgc atgccgtgtt tcgccaggca atccgtttcg tccgaaagat cttccgccac
     2101 gtggatgtgc accccggcgc cggtctccgc gcaaattcgc gccgtggcct ccagcgtggc
     2161 atcgcccagc gtcatttgcg catgcaagcc gaacagcgcc gtgatttggc cgtcgcccgc
     2221 gcgcgtcttt ttgatgaagc gctcgttttc cgccacgccg tccgccggga tgttgcggtc
     2281 cgacgtctca tagcacaggc agcccgacag ccccgccgcg cggaaggttt cctccatgac
     2341 atccagcgag ccatccgtgc acgacggcga cgcatggtgg tcaatgatcg tggtgcagcc
     2401 cgtgtggatg gcctccagca acgccacata cgacgaataa cgcaccgctt ccgcatccag
     2461 cgccaaatcc aacttccacc acatccgctc caggatttgc acaaagttgc tcgccggctc
     2521 gcccggcggc gcgaagccgc gcgcaaagga agaatacaaa tggtggtgcg cgttgatcag
     2581 ccccggcatc gccaccatgc cgcccgcatc cagcacctcg gcgcccgccg gcgcctccac
     2641 tttggcgccc accgccgcaa tcttgctacc ctcaatcagg atttcgccat ggggaatgac
     2701 tggttgcgcg tcgtcgttgg tcagcaccag cgcgttacga atgactaaag ccatgatgtt
     2761 ctctcctgtg gcccgggttt tccatgctgt ggaaaaaagt tcatcccgcg tgggcggggt
     2821 tttccacact gtggaaaaat ccggcccaat ttttccacac cgtggaaaaa cctctagcat
     2881 tttttccacg ctgtggaaaa gcttttacac cgcgttgggc atctggggcc gcagcatgtg
     2941 atacagcgcg ccggccgcca ccgcccccag gccatccgcc agcatatctt tttgcgcgtc
     3001 ccacacatcc ccctgcgatc ccagaaatgc cgtgcccgcc gtcggatccg cgagcaccgc
     3061 gtagagccac tccaccatct cgaacagcgc cgccaccgcc agaatggccg ccaatccgaa
     3121 gacgaggctg aacgcccggt tccggctcaa cccgcggcct tccacaaact ccagaatcgg
     3181 aaaaatccag aaccccaccg tgaaatgcgc cagccggtca aaatgattcc gctgcgcccc
     3241 gagccaatcc gtcacccagc caaacggcac ctcggcgaac gtgtaatgcc cgccgatcgt
     3301 gtgcagaaac accagcaccg acatcagcac ataagccgta ttggaaaagc ggatgccttt
     3361 gacgtacagc cacaccaccg gcgtcaccac cacccagacc gtaatattct ccgcccacca
     3421 cgtggcgcgc gacaccggcc caaccgccag caccacactc tccaccaccc acgccccaaa
     3481 caatataatc ggcaccgcgt tcttcatgcc ccgcatcata tccccgaccc cgccaaccgc
     3541 catcttttat tccatgcaaa agtgtccaca gtttataaac tctggacagc accctaaaaa
     3601 catcagtttt aaaatagcca taaatataga cctaacatat tgtgtataaa cataatatga
     3661 atatactaaa gaatgtccac actttataaa ctctggacat aagttggttt tggtccaaaa
     3721 aaacggggtt ggcgtgggcc aaccccgtgt gcatcatttc tgatgtgcga tgggcgttag
     3781 aacttttccg ggaaggcggc gatgaggccg tcggagaggg cgtcggcaag catcatcatg
     3841 tgggcttcgg ctgcgccgta tgcctcgatg ccttccgcgt acttgccctg gaggatggca
     3901 gtcgcataca ccagcgtggt gtcaatatgg ctcttcagca tgtcgcggac aaacgcctgc
     3961 ggccagtgct cgttggcggc ggagaggaaa tcggcgattt cctgcgcgtt gtcatacgcc
     4021 gccttgatgg cgttgttcag gccttcctca tcgttgccag ccttcgctac ttcgacgatt
     4081 ttaacggcac cggcaatgtg ctccttgagc agcttggtca cggcgtcgcc ggcttccttg
     4141 ccatagaaca gggcgaccgc gccgccgatg tcttcctggt tgcgcatcag ccgtgcggcc
     4201 gtggcttcaa acgcaggagt gccgtgcgcg gtgaccgcgg cataggtcca ttccatgtgc
     4261 tgggcccaga gttcgcgcat ggtgtcatgc agcttgttgc cggccgcgga acggacggca
     4321 gaagaggcat cgtgcgacga atggccggaa tcttcggcca cggcgcaggc gctcaagagg
     4381 cccatgctgg cgaccaaagc caccacaaat gcgactttta ctgttttttg gatgttcatt
     4441 ttgtctcctt gggtttgggg ttctctattt tttcggtaga gaacttaccg ctggtttgcg
     4501 gggtgtcaaa aaattatccg atatgatgga agttccacgg tgtggaaaaa agttttccac
     4561 agtgtggaaa atggctaggg tgctacaaat ggagacgatt gacggcaaac tggttgcgct
     4621 ggatgcgcgc tgggtgtttc gcgagttgag cggcattggc cgctacacgc tggagctgtt
     4681 gcgtcagttg ggcgatttgg gcggggggtg gcgttttttg gtgctggtgc gagatgcgga
     4741 gcgccgggag tttgtggaag cggccgcggg gctggggggg cgggggaata tggagttcgc
     4801 ggaggtgccg tttggaccgt tcgcgctgcg cgggcaatgg tcggtggcgc ggttgctgcg
     4861 ggcgcgcggg gtggcggtgt accactcgac caatttcatg attccactgc cggcgttttc
     4921 gcgccgtgag ccccgggcga tccgcgcggt gtgcaacatc catgatttga ttccgatggt
     4981 gcatccggag tttacgccgc gcgcgctcaa gacccggttt tttccggtgt accgggcgtt
     5041 gatgcacgaa attgcgcggc gggtggaccg ggtgctgacg gggagcgaat cggccaagcg
     5101 cgacatccag cgattgctgg gggtggcgga gtcgcggatt cgggtggcgg cggatggcgt
     5161 ggatgcgcgc tatgtgccgg gcggcgaaaa gccgtccgcg cgcggcgggc ccaaaaccat
     5221 cctttatgta ggccgcagcg atccctacaa aaatttgccg gggctggtgg cggtggtggc
     5281 acggctgatc gaagaggggc tgccggtgcg cctacggatt gcgggaccgc cggatgcgcg
     5341 gtatcccgcg gcggcagaat tggcgcagcg gctgaatgtg gcggcgcacg tggagtggct
     5401 gggctatctg gacgaggcag gattgctccg cgcgtatcag gaggcggacg tgctggcgct
     5461 gctctcgcgc tatgagggct tcggcctgcc ggtgctggag gcgatggcgt gcggcacgcc
     5521 ggtggtttgc tcgaatgtgg cgagtttgcc ggaggtggcg ggcgacgcgg
//
LOCUS       DAOPNP010000132         5283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_17067_length_5283_cov_7.511859, whole genome shotgun sequence.
ACCESSION   DAOPNP010000132 DAOPNP010000000
VERSION     DAOPNP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17067_length_5283_cov_7.511859"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..590
                     /locus_tag="PKI64_09810"
     CDS             78..590
                     /locus_tag="PKI64_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOE01332.1"
                     /translation="MSGELRVVVGDITKVAVDIIVNAANTTLLGGGGVDGAIHRAAGP
                     ELLAECRTLGGCATGDAKITKAYRLPARWVIHTPGPVYRDGQHGEPELLASCYRRAME
                     LAAEKQVKSIAFPCISTGVYRFPAENAAKIAISTVKSALDLCSGDLTVIFCCFSEKDA
                     KIYEKTLKNG"
     gene            743..3817
                     /gene="secA"
                     /locus_tag="PKI64_09815"
     CDS             743..3817
                     /gene="secA"
                     /locus_tag="PKI64_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOE01333.1"
                     /translation="MQWLLKRIIGTKNERDVRKLRPLVKRINELEVELQSLTAEQLQA
                     KTGEFRARLAKGATLDDLMCEAFAVVKNACRRLCGTVVEVCGHQQTWDMVPFDTQLIG
                     GMVLHQGKIAEMATGEGKTLVATLPVYLNALTGKGIHVVTVNDYLARRDATWMGTVYQ
                     YLGLTVGCIQNQMRPEERRAQYACDITYGTNSEFGFDYLRDMGMAYSPQEMVQRGWNY
                     AIVDEVDSILIDEARTPLIISGPAPYSTEQYQLVQPAVERMVRKQRDLCTRLMAEAKA
                     AMDKGDDDSAMRRLYQVSQGMPKNKQLLHLLEEPAGRRLLEKVQAMMLVDMNKELARE
                     LREELFFVIDEKSNDASLTDKGCNAMNPADPDYYVVPDLVSALAELDGDTTLSDQQKF
                     ERRQELQNQFADKSERIHAVDQLIRAYSVYERDVQYVIQDNQVVIVDEFTGRLMPGRR
                     WSDGLHQAIEAKEGVRIERETQTLATITIQNYFRLYQKLAGMTGTAETEADEFQSIYK
                     MDVVVIPTNRPVRRVDGNDQIYKTQREKFNAIIAEISACYQRKQPVLVGTISVDMSEI
                     LSRMLRRVNIPHNVLNAKNHQREAEIVANAGQPGAITIATNMAGRGTDIKLGPGVIWT
                     DKELITGPTRLTDKVDGKQTLRQMIEERPCGLYVIGSERHESRRIDRQLRGRCARQGD
                     PGMSRFFVSLEDDLMRLFGSDRISGIMEKLGIQEGEVLEHRWLNRSIETAQRRVEQQN
                     FAIRKRTLEYDDVMNKQREIIYGFRSQIVAAEQVRDHLLDVVQDVIYARADDLDDDEE
                     SLAAFATWVNSQFPIGFHPEQAVRKEGKRGRSVLDAEGTARAVFGRVGKAYAEKCLLE
                     DPAQINSLERFIMLNAVDTQWQEYLRNMDTLRQGVGLRAYGQRDPLVEYKNEAYTLFA
                     DLMDRIKGEIAGRIFRATTSPRAYQNFLGDLHRMAQAQHAEMAPLVGGRSATTAAPAR
                     GAADSGAESAMNAALAASRAQPAKREGPKVGRNDPCPCGSGKKYKKCCGKDA"
     gene            3814..5202
                     /gene="pcnB"
                     /locus_tag="PKI64_09820"
     CDS             3814..5202
                     /gene="pcnB"
                     /locus_tag="PKI64_09820"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOE01334.1"
                     /translation="MKDDPVILTPDQHGLKQEDIAWEALRVLTRLDEAGYKAYLCGGG
                     VRDLLLKRKPKDFDIATDARPEEVKKLFRNCRIIGRRFKLAHVFFRDVIIETATFRAL
                     LDNPPPEAEGVPLPSRRRQDVPDPIFATRDGVIVRDNVYGSPEEDARRRDFTINGLFY
                     NIADGTIIDYVGGLEDLQRRVVRVIGDPAVRFHEDPVRMVRAVRIAAQLDFEIERQAR
                     DAIVEMAGELKYSSRERLHEEILKILNCGCALKAFERAWRKGLFQTIYPAYAQWLQGP
                     DRQPAMQWIKKALRQFDVWKQAGLKPMPALQHALLFGPFLEGLAADNTAEDLPEFEAT
                     LQAVNTVLRDKSNLTQIPKAVVYDVERILGIQVQMRKSSGQSKYATRLRHRNGFNEAL
                     IYLKFAGGVDPSRKELLARWMTPPAPHHNPPARPNAPPSAPEHPTRAQPAASAHEHKP
                     RAIPSVPAPAAE"
BASE COUNT         1152 a         1532 c         1624 g          975 t
ORIGIN      
        1 aagccgagct ttctttgtga cataatgtca cttatatatg caagactcaa tagaaaaacg
       61 aacaatcagg ggcaggaatg agtggtgagt tgcgagtggt ggtgggggat attacgaaag
      121 tggcggtgga catcatcgtc aatgctgcga acacgacact gctgggcggc gggggtgtgg
      181 atggggcaat tcaccgggca gcggggccgg aattgttggc ggaatgccgc accttgggcg
      241 gttgcgccac gggggatgcc aaaatcacca aggcgtatcg tttgccggcg cgttgggtga
      301 ttcatacacc ggggccggtc tatcgtgatg gtcagcatgg cgaaccggag ctgctggcaa
      361 gctgctatcg gcgcgccatg gagctggcgg cggaaaaaca ggtaaaatcc atcgcttttc
      421 cttgcattag cacgggggtc tatcgttttc cggcggaaaa tgcggccaaa atcgcaattt
      481 ctaccgtaaa aagtgcattg gacttgtgtt caggcgattt gacggtcatt ttttgctgtt
      541 tttcggaaaa agacgccaaa atatacgaaa aaacactaaa aaacggctaa taattggcaa
      601 aaatggggca aaatccctaa aaatagctat tttggcagat ttttgggccg tggttcggtg
      661 cttgtcaggg cggggcgatg gggggtaaag tctcccgatt tattattccg gttgggtttt
      721 ccatcggcgg aagcgagatt ctatgcagtg gcttttgaag cgaattattg gcaccaaaaa
      781 tgagcgtgac gtacgcaagt tgcgtccgct ggtcaagcgc atcaatgagc tggaagtgga
      841 gttgcaatcg ctgacggcgg agcagttgca ggcgaagacg ggggagttcc gcgcgcggct
      901 ggccaagggg gcgacgctgg atgatttgat gtgcgaggcg tttgccgtgg tgaagaatgc
      961 ctgccgtcgg ctgtgcggca cggtggtcga agtgtgcggc caccagcaaa cgtgggacat
     1021 ggtgccgttc gatacgcagt tgatcggcgg catggtgctg caccagggca agattgcgga
     1081 aatggccacc ggcgaaggca aaacactcgt ggccacgttg ccggtgtatc tcaacgcgct
     1141 gacgggcaag ggcattcacg tggtgacggt caacgactac ctcgcgcggc gcgatgccac
     1201 gtggatgggg acggtgtatc agtatctggg gctgacggtg ggctgcatcc aaaaccagat
     1261 gcgcccggaa gaacgccgcg cccagtacgc gtgcgacatc acttacggca ccaattccga
     1321 gttcggcttc gattacctgc gcgacatggg catggcctac agcccgcagg aaatggtgca
     1381 gcgcggctgg aattacgcca tcgtggacga ggtggactcg atcctgattg acgaggcgcg
     1441 caccccgctg attatttccg gccccgcgcc gtattccacc gagcaatatc aactggtcca
     1501 gccggcggtg gagcgcatgg tgcgcaagca acgcgatctg tgtacccgcc tcatggccga
     1561 ggccaaggcg gccatggaca agggcgatga cgattctgcc atgcgccggc tgtaccaggt
     1621 cagccaggga atgcccaaaa acaagcagct cctccacctg ttggaggagc ccgccggtcg
     1681 ccgcctgctc gaaaaagtgc aggccatgat gctcgtggac atgaacaagg agctcgcccg
     1741 ggaactgcgc gaggagttgt tcttcgtcat tgacgaaaag agcaatgatg ccagcctgac
     1801 ggacaagggt tgcaacgcga tgaatccggc agacccggat tattatgtgg tacccgattt
     1861 ggtgtccgcg ctggccgagt tggatggcga taccacgctg agcgaccagc agaagttcga
     1921 gcgtcgccag gagctgcaaa accagtttgc cgacaagagt gagcgtattc acgccgtgga
     1981 ccaactgatc cgggcatata gcgtctatga gcgcgacgtg cagtacgtca ttcaggacaa
     2041 ccaggtagtg attgtggacg agttcaccgg ccgcctgatg cccggacggc gctggagcga
     2101 cggcctgcac caggccatcg aagccaagga gggcgtgcgc atcgagcgcg aaacccagac
     2161 gctggccacc atcaccattc agaactattt ccgcctttat caaaagttgg cgggcatgac
     2221 tggcactgcc gagaccgagg ctgacgagtt tcagtccatc tataaaatgg acgtggtggt
     2281 catccccacc aaccggcccg tgcggcgggt ggatggcaac gaccagattt acaagaccca
     2341 gcgtgaaaag ttcaacgcca tcatcgccga gatttccgcg tgctatcaac gcaagcagcc
     2401 ggtgctggtg ggcaccattt cggtggacat gtcggaaatc ctcagccgga tgttgcggcg
     2461 cgtgaacatt ccccacaacg tactgaatgc caaaaaccac cagcgcgaag ccgaaatcgt
     2521 cgccaatgcc gggcagcccg gcgccatcac catcgccacc aacatggccg gccgcggcac
     2581 cgacatcaaa ctaggccccg gcgtcatttg gacggacaaa gagctcatca cgggccccac
     2641 gcggctcacg gacaaggtgg atggcaagca aaccctgcgt cagatgatcg aagaacgtcc
     2701 ctgcggactc tacgtgatcg gcagcgaacg gcacgaatcg cggcgtattg atcgccagct
     2761 ccgcggacgc tgcgcgcgcc aaggcgaccc gggcatgtcg cggttcttcg tctcgctgga
     2821 agatgatctg atgcggctgt tcggctctga ccgcatttcc ggcattatgg agaagttggg
     2881 cattcaggaa ggcgaggttc tcgaacaccg gtggctgaac cgctccatcg aaaccgccca
     2941 gcggcgcgtg gaacagcaaa atttcgccat ccgcaagcgc acgctggaat atgacgacgt
     3001 gatgaacaag cagcgcgaaa tcatttatgg tttccgcagc cagattgtcg ctgctgaaca
     3061 agtgcgcgac cacctgctgg atgtggtgca ggatgtgatc tacgcccgcg ccgatgattt
     3121 ggacgacgat gaggaaagcc tcgccgcgtt tgccacgtgg gtcaacagcc agtttcccat
     3181 tggcttccat ccggaacagg ccgtgcgcaa ggaaggtaag cgcgggcgca gcgttttgga
     3241 tgccgagggt accgcgcgcg ccgtgtttgg gcgcgtgggc aaggcttacg ccgaaaaatg
     3301 cttgctggaa gaccccgcgc agatcaattc ccttgagcgt ttcatcatgc tcaatgcggt
     3361 ggatacccag tggcaggaat atctgcgcaa catggacacc ctgcgccaag gtgtgggtct
     3421 gcgcgcctac ggccagcgcg acccgttggt ggaatacaag aacgaggcct acaccctgtt
     3481 tgccgacctg atggaccgca tcaagggcga gattgccggc cggattttcc gcgccaccac
     3541 atcaccccgc gcctaccaga atttccttgg cgatctgcac cgcatggcgc aggcgcagca
     3601 cgcggagatg gcgccgctgg tcggtggtcg gtccgcgacc acggcggctc ccgcgcgcgg
     3661 cgcagcggac tccggcgcgg agtccgcgat gaatgccgcg ctggccgcat cccgggcgca
     3721 acccgccaag cgcgaagggc ccaaggtggg ccgtaacgat ccctgtccct gcggcagcgg
     3781 caagaaatac aagaaatgtt gcggcaagga cgcatgaagg acgatcccgt catcctgacc
     3841 cccgatcagc acggcctgaa acaggaggat attgcctggg aggccctgcg ggtgctgacc
     3901 cgcttggatg aggccggcta caaggcctat ttgtgcgggg gaggggtgcg cgatttgctg
     3961 ctcaaacgca agcccaagga tttcgacatc gccaccgatg cccggcccga ggaagtcaaa
     4021 aagctattcc gcaactgccg gatcatcggc cgccgcttca agctggcgca cgtttttttc
     4081 cgcgatgtta tcatcgaaac cgccaccttc cgcgcgctgc tggacaaccc gccgccggaa
     4141 gccgagggcg taccgttgcc ctcgcgtcgc cggcaggatg tgccggaccc catcttcgcc
     4201 acgcgcgacg gcgtaattgt ccgtgacaat gtgtacggct cgccggaaga agatgcccgc
     4261 cgccgcgact tcaccatcaa cggcctattt tacaacatcg ccgacggcac catcattgat
     4321 tacgtcggcg ggttggaaga tcttcaacgg cgcgtggtgc gcgtgatcgg cgaccctgcc
     4381 gtccgcttcc acgaagaccc cgtgcggatg gtccgcgccg tccgtattgc cgcgcagctc
     4441 gattttgaaa tcgaacggca agcccgcgac gccatcgtgg aaatggccgg cgaactcaaa
     4501 tactcctcgc gcgagcgctt gcacgaagaa atcctgaaaa tcctgaattg tggttgtgcg
     4561 ctcaaagcct ttgagcgcgc ttggcgcaag gggttgttcc agaccattta tccggcctat
     4621 gcccagtggc tgcaaggccc cgaccgacag ccagccatgc agtggatcaa aaaggcgctg
     4681 cgtcagttcg atgtctggaa acaggccgga ctcaaaccga tgcccgcgct acagcacgcg
     4741 ctgttgttcg gtccattctt ggaaggactg gccgcagaca ataccgccga agacctgccc
     4801 gaattcgaag ccacgctcca agccgtcaat acggtgctgc gcgacaagtc caacctgacg
     4861 caaattccga aagccgtcgt gtacgatgtg gaacgtattt tgggcattca ggtgcaaatg
     4921 cgcaaatcgt cgggccagag caaatatgca acccgcttgc ggcaccgcaa cggcttcaac
     4981 gaagcgctca tttatttgaa atttgccggc ggtgtggacc cctcgcgcaa agagctgttg
     5041 gcccggtgga tgacgccccc tgcgccgcac cacaatcccc ccgcacgccc caacgcgcct
     5101 ccctccgcgc cggagcatcc cacgcgcgcg cagcctgccg cgtcggccca cgaacataag
     5161 ccccgcgcca tcccctcggt accggctcct gctgccgaat agtatctccg ggtacccggc
     5221 cggaaaaaat ggtgtcgtgg tgaaagcaaa ccttcacact ttatgtagtt agcaaagccg
     5281 cac
//
LOCUS       DAOPNP010000133         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_17412_length_5199_cov_8.092535, whole genome shotgun sequence.
ACCESSION   DAOPNP010000133 DAOPNP010000000
VERSION     DAOPNP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17412_length_5199_cov_8.092535"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKI64_09825"
     CDS             <1..708
                     /locus_tag="PKI64_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01335.1"
                     /translation="GIAPWAIMDLMAKTTEAMRETNWVWAAQCAATIGHYVGDLHMPL
                     HTTRNYNGQETWQHGVHSRVESEMTKYFFDVNSIQPEPAVYLEDPFSAIIEWTAHSAE
                     LARTILRADDMAKRTANGRIDTETYYHTLWDLTGDIINSQISHAITALSSLWYTAWVN
                     AGRPPIPPAFDELPTLSVHSGVGIDPRTPGDRIDAHGSQPISRYNIIIWSVMGFICLV
                     VIGSSIHRGIQARRAKK"
     gene            705..1472
                     /locus_tag="PKI64_09830"
     CDS             705..1472
                     /locus_tag="PKI64_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOE01336.1"
                     /translation="MKEPRAKRQRLDLLLVARGLAESREKAQRLILAGEVFVNDAPAT
                     KPGHEVPEDCALRVREPERFVSRGGLKLEAAFAQFAGLSAAGRVCVDIGASTGGFTDC
                     LLQHGAARVYAVDVGKLQLHPRLVADPRVVVLDGCNARNLQPGDLPETPDMAVTDVSF
                     ISLQLILPAIDRLLKPGGEVVALIKPQFEAGREEVGKGGVIRDPALRQRIVARIREFG
                     TQTLGWQWLGGCESPIQGPAGNVEFLSYWQKPAAPRV"
     gene            complement(1495..2553)
                     /gene="argC"
                     /locus_tag="PKI64_09835"
     CDS             complement(1495..2553)
                     /gene="argC"
                     /locus_tag="PKI64_09835"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOE01337.1"
                     /translation="MIKVKVVGCGGYGGVGIVELLLRHPHVKIQTLVAATETGMLMSD
                     LYPHLKGFCDLRIHKPDEPAAQEPADVVFFSTPDGVGMVQAPAELAKGARVIDYSGDF
                     RFNNTADYAEYARRIGRDPVHKAPELLPQTVYGLPELNRQNYAKDQRLVGNVGCFAMS
                     CLLGLAPAAAAKLIDFRSVICDCKTGVSGAGKKATPIHHYPARYENMQAYRLSGHQHV
                     MEIERMLGQLADEAVPVTFTAQVLPICRGIMACLYGTLRDGITREQVWAAYADYHRAN
                     SFVRLYDSKTAIGTANVRGSNFCDLVVDVDERTRRLRVISHIDNLVKGQAGNALQNMN
                     LICGFPENTGLDFPGSYP"
     gene            complement(2572..3639)
                     /locus_tag="PKI64_09840"
     CDS             complement(2572..3639)
                     /locus_tag="PKI64_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOE01338.1"
                     /translation="MDAHSLDFLKRLMGAISPSGHELDAVRVFRDEARQFAADVRTDV
                     HGNTDVVINPGGSPRVMLAGHYDEIGFIVTYIDPKGFLWISPIGGWDEQIPQGQRVSI
                     RTAQGVVPGIIGKVPIHVQKKEARSRVTPLTDLWVDIGARTAREAEKLVAVGDPMVID
                     QGVVELPNGRLAGRAFDNRAGAFVVLEAARLLAQLNPAAEIHAVGTVQEEIGSRGATT
                     SAFGIAPEVGIAVDVTFATDHPMMDDTQKHRGKVALGRGPVISRGANVNPALFSHLVA
                     VAAAQKIPVQIVAEGRATATDADPIQISRAGVATALVSIPLRYMHTPGEIVDIKDLSG
                     LARLLAQAVLAMKPGQSWIPF"
     gene            complement(3666..5090)
                     /locus_tag="PKI64_09845"
     CDS             complement(3666..5090)
                     /locus_tag="PKI64_09845"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOE01339.1"
                     /translation="MNRLSSIAMVVLLALAGWLAQPLPAVAQVRTLKAVTYAGKRYVA
                     LQDLAAMYGLPLTTPGGRTLLIRGQYTSLQFTTDSREAMLNGCKVWLHMPVIKVRGLW
                     SLSDADAQVVVDPVVRPSAYLGARGSRTVVLDPGHGGKDPGTQGGGYKEKDLVLDIAL
                     RVKAHLAAAGIRVVLTRDADRFWELTDRPYLAARGGGDAFVSIHLNSAAARTVQGVET
                     FVTAAENFPPTAESKTTGRYPAVPNNQFNHSNMLLGSQIQRALVGVTRADDRGLKHAR
                     FVVLRNSAMPAALVECGFLSNAQEAQKLATPSYRESVALGIAQGIMNYFALVNRARVE
                     LGAPLIQKPAQLAAPLAPVPPPAPLPPVPMASRAAATSVVVSPPAAPAPATVAPAPAP
                     VPPLPAPPAPSVPPASTPPPSPAAPVPPPAAAPEPPAPPPAEPSPPAVKVREPAVVVR
                     PLQPVAPPPAKLLNPNFARSAPPK"
BASE COUNT          959 a         1689 c         1619 g          932 t
ORIGIN      
        1 ggcatcgcgc cgtgggccat catggatttg atggccaaaa ccaccgaggc catgcgcgaa
       61 accaactggg tatgggccgc gcaatgcgcc gcgaccatcg gccattatgt cggcgacctg
      121 cacatgccgt tgcacaccac ccgcaactat aacgggcagg aaacctggca gcacggcgtc
      181 cactcgcgcg tggaatcgga aatgacaaaa tattttttcg atgtgaacag catccagccg
      241 gaacccgcgg tgtatctgga agatccattc tcggccatca tcgaatggac cgcgcattcc
      301 gctgaattgg cccgcacaat cctgcgggcg gacgacatgg ccaagcgaac agccaacggc
      361 cgcattgaca cggagaccta ttaccatacg ctctgggacc tcaccggcga catcatcaac
      421 agccagattt cccacgccat tacggcattg tcatcgctgt ggtacacggc atgggtcaac
      481 gccggcaggc cccccatccc gccggcgttc gacgaattgc ccacactttc cgtgcattcc
      541 ggcgtgggca ttgacccccg caccccgggc gaccgcattg acgcgcatgg cagccagccc
      601 atctcacgct acaacatcat catttggagc gtgatggggt tcatctgcct ggttgtcatt
      661 ggctcttcaa ttcatcgcgg cattcaagcc cgccgcgcca agaaatgaaa gaaccgcgag
      721 cgaagcgcca acgactggac ctcttgctgg tggcgcgcgg cttggccgaa agccgcgaaa
      781 aagcccagcg cctgattttg gcgggcgagg tttttgtgaa cgacgccccc gccaccaagc
      841 cgggccatga ggtgcccgaa gattgcgccc tgcgcgtgcg cgagccggaa cggtttgtca
      901 gccggggcgg gttgaagctg gaagcggcgt tcgcccaatt cgccggcttg agcgcggcgg
      961 gcagggtgtg cgtggacatc ggcgcctcca ccggcggctt caccgattgc ctgttgcagc
     1021 acggcgctgc gcgcgtctat gcggtggatg tgggcaagtt gcagttgcat ccgcgccttg
     1081 tggctgaccc gcgcgtggtg gtgctggatg gttgcaatgc ccgcaatctg caacccggcg
     1141 atttgccgga aacgcccgac atggccgtga cggatgtttc tttcatttcc ctgcaactga
     1201 ttttgccggc cattgaccgg ctgctgaaac ccggcggcga ggtggtggcg ttgatcaaac
     1261 cgcagtttga ggccgggcgc gaagaagtgg gcaaaggcgg cgtaattcgg gacccggcgc
     1321 tgcgccagcg aatcgtggcg cggattcgcg aattcggcac gcagactctc ggctggcaat
     1381 ggctgggcgg ctgcgagtcg cccatccaag gccccgcggg caacgtggag tttctgagct
     1441 actggcaaaa gcccgccgcg cctcgggttt aaccacggcg ggcggctcaa ccgcctacgg
     1501 gtaggaaccc ggaaaatcca aaccggtatt ttcagggaag ccgcaaatca agttcatgtt
     1561 ttgcagcgcg ttgccggcct gccccttcac gaggttgtca atgtgcgaaa tcacccgcag
     1621 gcggcgcgta cgctcatcca catccaccac caagtcgcag aaattgctgc cgcgtacgtt
     1681 ggcggtgccg atggccgtct tgctgtcata gagtcggaca aacgagttgg cgcggtgata
     1741 gtcggcgtag gccgcccaca cctgctcgcg ggtgatgccg tcgcgcaacg tgccatagag
     1801 gcaggccatg attccgcggc aaatgggcag cacctgcgcg gtgaacgtca ccggcacagc
     1861 ctcgtcggcc agttggccga gcatccgctc gatttccatg acgtgctgat ggccgctgag
     1921 gcggtaggcc tgcatgttct cgtagcgcgc cggatagtgg tggatcggcg tggctttttt
     1981 gccggcgccg gacaccccgg ttttgcagtc gcaaatcacg ctgcggaaat caatgagctt
     2041 ggcagcggcg gccggcgcaa gccccagcag gcaactcatg gcaaagcacc ccacgttgcc
     2101 taccagccgt tgatccttcg cgtagttttg gcggttcagc tccggcaggc catagacggt
     2161 ttgcggcagc aactcgggcg ctttgtgcac gggatcacgt ccgatgcgcc gggcatattc
     2221 ggcatagtcc gccgtgttgt tgaaccggaa gtcgccgctg taatcaatca cacgcgcgcc
     2281 cttggccagt tccgccggcg cctgtaccat acccacgccg tcgggcgtgg agaagaagac
     2341 tacatccgcc ggctcctgcg cggcgggttc atcgggcttg tgaatgcgca aatcgcagaa
     2401 gcccttgagg tgggggtaca agtcgctcat cagcatgccg gtttccgtcg cggccacgag
     2461 cgtttggatt ttcacgtgcg gatgccgcaa cagcagctcc acaatgccca ccccgccgta
     2521 gcccccgcaa ccgaccactt tgaccttgat catggtgtca tcctccactg tctaaaacgg
     2581 aatccaactt tgccccggtt tcatggccag caccgcctgc gccagcagcc gggcaagacc
     2641 ggacaaatcc ttgatgtcca cgatttcgcc cggcgtgtgc atgtagcgca gcgggatgct
     2701 caccagcgcc gtggccacgc ccgcgcggct gatttgaatc gggtcggcat ccgtggccgt
     2761 ggcccgacct tccgccacga tctgcaccgg gattttttgc gcggcggcaa ccgccaccaa
     2821 atggctgaac agcgccggat tgacgttggc tccgcgcgaa atcaccggac cgcggcccaa
     2881 cgccaccttg ccgcggtgct tttgggtgtc gtccatcatc gggtggtccg tggcaaaggt
     2941 gacatccacc gcaatgccga cttccggggc gatgccaaag gccgatgtgg tcgcaccccg
     3001 gctgcctatt tcttcctgca ccgtgccaac ggcatgtatt tccgcagccg ggttgagctg
     3061 cgccaaaagc cgcgcggctt ccagcaccac gaatgcgccg gcccggttgt caaatgcccg
     3121 tccggccagc cgcccgttgg gcagttccac cacgccttgg tcaatcacca tgggatcgcc
     3181 caccgccacc agtttctcgg cttcccgcgc cgtgcgcgcg ccgatgtcca cccacaggtc
     3241 cgtcagcggc gtcacccggc tgcgcgcttc ttttttctgc acatggatgg gcaccttgcc
     3301 gatgatgccc ggcaccacgc cctgcgccgt gcggatcgaa acgcgctggc cctgcgggat
     3361 ttgttcatcc cacccgccaa ttggtgaaat ccagaggaat cccttcgggt caatataggt
     3421 gacaatgaag ccgatttcgt cgtagtgccc ggccaacatc acacgcggcg agccgccagg
     3481 attgatgacc acatcggtgt tgccgtgcac gtcggtgcgc acatcggcgg caaactgccg
     3541 ggcttcgtcg cgaaagacgc gcacggcatc cagctcgtgg ccggaaggcg aaatggcgcc
     3601 catcaatcgt ttcagaaaat ccaggctatg cgcatccatg gtcatgtcct tggaaataat
     3661 atcctctatt tgggcggggc gctgcgagca aagttgggat tgagcaattt ggccggcggc
     3721 ggcgcaaccg gctgcaaggg gcggacaaca actgccggct cacggacttt gacggctggc
     3781 ggtgaaggtt ctgccggcgg cggcgcgggc ggttcaggag ccgcggcagg tggcggcacg
     3841 ggcgcggccg gtgacggtgg cggagtggat gccggaggga cactcggcgc aggcggagca
     3901 ggcagcgggg gcaccggtgc cggtgccggc gcaactgttg ctggtgccgg tgcggccggc
     3961 ggcgaaacca ccaccgatgt cgcagccgcc cgtgaagcca tcggcaccgg cggcaacggc
     4021 gcgggggggg gcactggtgc cagtggcgcg gccagttggg ccggcttctg gatcaacggc
     4081 gcgcccaact ccacccgcgc gcggttcacc agcgcaaagt aattcatgat gccctgggcg
     4141 atgcccagcg ccaccgattc gcgatacgac ggcgtggcca gtttttgtgc ttcctgcgca
     4201 ttggacaaaa aaccgcattc aaccagcgcc gccggcatgg cggagttgcg caacaccaca
     4261 aaccgcgcgt gcttgaggcc gcggtcatcc gcccgcgtca cgcccaccaa cgcccgctga
     4321 atctggctgc ccaacagcat gttcgagtga ttgaactggt tgttcggcac cgccggatag
     4381 cggccagtgg ttttggattc cgcggtcggc ggaaagtttt ccgcagcagt cacaaaagtt
     4441 tccacgccct gcactgtgcg cgcagccgca gagttgaggt ggatgctcac aaaggcatcc
     4501 cccccgcccc gcgcggccag ataaggcctg tcggtcagct cccaaaaacg gtcggcgtcg
     4561 cgcgtcaaga ccacgcggat gcccgccgcc gcgaggtggg ccttcacgcg caaggcaatg
     4621 tccagcacaa ggtctttctc cttatagccg ccaccctgcg tgccggggtc cttgccgccg
     4681 tggccaggat ccagcaccac cgtgcgcgag ccgcgcgcac cgagataggc cgagggccga
     4741 accaccgggt ccaccaccac ctgcgcgtcg gcatcgctca acgaccagag gccgcgcacc
     4801 ttgatgacgg gcatatgcag ccacaccttg cagccgttca gcatggcctc gcggctatcc
     4861 gtggtgaatt gcaggctggt gtattgccca cgaatcagca gagtgcgccc gccgggggtc
     4921 gtcaagggca ggccgtacat cgccgccaaa tcttgcagcg ccacgtagcg cttgccggca
     4981 taggtcacgg ccttgagggt gcgcacttgg gccaccgccg gcaacggctg cgccagccag
     5041 ccggccaagg ccagcaggac aaccatcgcg atggagctca accgattcat aaaggcgctc
     5101 accttacggc agccaacgcc caaaggcaat ctttccatgc acgggtgccg tgtagttttc
     5161 aaacccgcac ttttttgtag ttagcaaagt cgcactttt
//
LOCUS       DAOPNP010000134         5189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_17449_length_5189_cov_7.127970, whole genome shotgun sequence.
ACCESSION   DAOPNP010000134 DAOPNP010000000
VERSION     DAOPNP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_5189_cov_7.127970"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..940
                     /locus_tag="PKI64_09850"
     CDS             197..940
                     /locus_tag="PKI64_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOE01340.1"
                     /translation="MPKCRHLILRSAAWWIAALLLCATGCQRDSAAREARERNLRRAK
                     AAVQAQDISAAIHWGERAVARRPNSGLAHRELGLLYDNWQQDYVRALYHYQRYLELRP
                     DAADHADVADRMAHCRTALAAEVAATPAEMKRAIQTRDSRITALETELAALRADAATS
                     AKPPRGKPSAAKAAPAAPPATTAPVAPPPAAAETQTHVVQSGETLATISARYYGSPAK
                     WELIFNANRDRLSSPNNVRVGTRLTIPPP"
     gene            953..1684
                     /locus_tag="PKI64_09855"
     CDS             953..1684
                     /locus_tag="PKI64_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01341.1"
                     /translation="MGTDFFDDDLVRQREAALKIKLDADSAAPRDGHIPSSRDLPNRP
                     VSDLTLTHLARHKQEVTDQMVDKAQELDRLRARQEELERERRLLEELRQKQEAFDSGR
                     RELKTRLQEALVRMEKDQLQADRLSQLLEETRAQFNDRLATVAALNEDDWTNDTLLDE
                     LNKALATLENVRADYNKALAKLEAVQPHAADAPRPLAFSAATPAPAAHTFAYWWRAGF
                     AFLLPFMVFVALLFFIGLLSYYFWL"
     gene            1686..2225
                     /locus_tag="PKI64_09860"
     CDS             1686..2225
                     /locus_tag="PKI64_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01342.1"
                     /translation="MADRRNMSAWERRLKEIEREKAELRTQIRAVERWPDAPQPGTPP
                     SEPRLRDAAIPVGYRGAHPALLRDNPELLEPGTADLPPGEIAVDFAPGPDGLATKRVV
                     MPRLQRTDLLRPATGGAPTAAGRLAEPRHDQLRNYYGVAGLKRVREARREQGSQRART
                     VFMVGMVLVLSYVLFKMLT"
     gene            2222..2743
                     /locus_tag="PKI64_09865"
     CDS             2222..2743
                     /locus_tag="PKI64_09865"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOE01343.1"
                     /translation="MTRPPQEPVQATLATTWGPVVITADAAGLLACHLPDAGTPRGPL
                     RTGRATLPPHAPPALRQAVAAAAALLAGRTPAQLPPLSPAAWADATAFQRAIWRAMQR
                     IPRGRTMTYAELARRAGRPRASRAAGGACGANPLPLFVPCHRVVATGGGLGGFSGGLA
                     WKVHLLRVEGARP"
     gene            2740..3819
                     /locus_tag="PKI64_09870"
     CDS             2740..3819
                     /locus_tag="PKI64_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOE01344.1"
                     /translation="MNIAVACGGTGGHVFPGLATARELLSRGHEVTLWLTGRDTEQAA
                     RQTWDGPVVEVPARGLPSGFSWQALQRAAQLGQAIWRCRARMRPARPAVLLAMGSYAS
                     VGPCGAARLLGVPIVLHEANVIPGRAVRLFARRAAAVAAGFEETRYHLPRARLHVVGM
                     PLRDELTAAAHALPPRPAGAPFRFLVMGGSMGARRLNQVVTAALAPLVAAGIPFAVTH
                     LAGRDDEPAVRAAYAAAGIPADVIAFTNYMAPLYHAADFAICRAGASTCAELAFFGVP
                     ALLVPYPHAAMDHQTANARAVEKRGAADVVAEADLTPAWLEAYLLQTLRKPARLARLR
                     DAALKAGARNGAADLATLVEQSARP"
     gene            3816..>5189
                     /gene="murC"
                     /locus_tag="PKI64_09875"
     CDS             3816..>5189
                     /gene="murC"
                     /locus_tag="PKI64_09875"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOE01345.1"
                     /translation="MTPPAEPWRAAIRRWLAQPAHVHLLGIGGIGMAGLARLLAARGQ
                     HVTGCDAATPRTLRWLQAQGIPATTGHDPAHLADAQWAIHSPAVPADHPEMLAAARLP
                     LFRRGQVLPVLAEQWQTIAVAGTHGKTTTAAMITHILRHTGADPSWCIGGELAPDHAP
                     AGIGTGPWLVIEADESDATLAHYAPAVALITNIEFDHMEHFTSPAHFTQCFRAYARQA
                     RHLVYCADDPAAAALGAEIGGLSYGLTPAADYRAENPQPTPAGTRATLRGTPLHIPIP
                     GPHNLLNALGAIAAVSHCGIDLSAAAAALATFALPRRRFEQIAHARGIRIIADYAHHP
                     AEIRALIAATRETGADRIWAIFQPHRYTRTRALGPDFPPAFDGITGLILLPVYAASEP
                     PLPGGTAADLLKHLWRHNQYPAELAADLPAAAHAAAARWQPGDTVLVIGAGDVEQIAP
                     HLQALLHA"
BASE COUNT          769 a         2265 c         1418 g          737 t
ORIGIN      
        1 accgcctccg gctaccgggg gcggtttttt tgttgccgcc acacgttgcc tcacctgaca
       61 aaagcatctg gtcggattgt ggggctggaa cggtcgcaga ccttccagcc gcgcccggtt
      121 cccgcacgca aaaatgcgcg cggggtgggg gagtatcgta attgattttg gcgtccaatt
      181 tgctacggtg ccggacgtgc caaaatgccg tcatctgatt ttacgttccg ccgcgtggtg
      241 gattgccgcg ctgctgctgt gcgccacggg ttgccagcgc gattccgccg cgcgggaagc
      301 gcgtgagcgc aatctgcgcc gcgccaaggc cgccgtgcag gcgcaggata tttccgcggc
      361 catccactgg ggcgaacgcg ccgtcgcgcg ccggcccaac tccggtctgg cccaccgcga
      421 actcggcctg ctctatgaca actggcagca ggattacgtg cgcgcgctct atcattacca
      481 acgctatctc gaactgcgtc ccgacgccgc agaccacgct gacgttgccg accgcatggc
      541 gcactgccgc accgcgctcg ccgcggaggt cgccgccacg cccgccgaaa tgaagcgcgc
      601 catccaaacg cgcgactccc gcatcaccgc gctggaaacg gaactcgccg cgttgcgcgc
      661 ggatgccgca acgtctgcca agccgccgcg cggcaagccc agtgcggcca aagccgcgcc
      721 tgccgccccg ccggccacca ctgcgcccgt cgcgccgccg cccgccgccg ccgaaacgca
      781 aacccacgtt gtccaatccg gtgaaactct cgccaccatt tccgcgcgct actacggctc
      841 gcccgccaag tgggagctca ttttcaacgc caaccgtgac cgtctttcca gccccaacaa
      901 cgtgcgcgtg ggcacgcgcc tgaccatccc cccgccataa ggagccgcca ccatgggtac
      961 tgattttttt gatgatgatc tcgtccgcca acgcgaagcc gcgctgaaga tcaagctcga
     1021 tgccgacagc gccgccccgc gcgatggcca catcccgtcc tcgcgcgatc tgcccaaccg
     1081 ccccgtctcc gatctcaccc tcacccacct cgcgcgccac aagcaggaag tcaccgacca
     1141 aatggtggac aaggcccagg agctcgaccg ccttcgcgcg cggcaggagg agctcgaacg
     1201 cgagcgccgc ctcctcgaag aactccgcca aaaacaggag gccttcgatt ccggccgccg
     1261 cgaactcaaa acccgcttgc aggaagcgct cgtgcgtatg gaaaaagatc aactgcaagc
     1321 cgaccgcctc agccaactgc tcgaggaaac ccgcgcgcag ttcaacgacc gcctcgccac
     1381 cgtcgccgcg ctcaacgaag acgactggac caacgacacc ctgctcgacg aactcaacaa
     1441 ggcgcttgcc acgctggaga acgtccgcgc cgattacaac aaggcgctcg ccaagctcga
     1501 agccgtgcag ccgcatgccg ccgatgcgcc gcgcccactg gccttcagcg ccgccacccc
     1561 cgcgcccgcc gcccacacct tcgcctattg gtggcgcgcc ggcttcgctt tcctcctccc
     1621 gttcatggtg tttgttgcgc tcttgttttt catcggcctg ttgagttatt atttctggct
     1681 gtagcatggc cgaccgccgc aacatgtccg cgtgggaacg gcgcttgaag gaaatcgagc
     1741 gcgagaaggc ggagctgcgc acgcaaatcc gcgccgtcga acgctggccc gatgccccgc
     1801 aacccggcac gccccccagc gaaccacgcc tgcgcgatgc cgccatcccc gtcggctatc
     1861 gcggcgcgca tcccgccctc ctgcgcgaca accccgaact cctcgaaccc ggcaccgccg
     1921 atctgccgcc cggcgaaatc gccgtggact tcgcgcctgg ccccgacggc ctcgccacca
     1981 agcgcgtggt catgccgcgc ctgcaacgca ccgatctgct gcgccccgcc accggcggcg
     2041 cgcccacggc cgccggccgc ctcgccgagc cccgccacga ccaattgcgc aactattacg
     2101 gcgtggccgg cctcaagcgc gtccgcgaag cccgccgcga gcaaggcagc cagcgcgcgc
     2161 gcaccgtgtt catggtgggc atggtgctcg tgctgagcta cgtgctcttc aagatgctca
     2221 catgacccgc ccgccgcagg agcctgtcca agccacgctc gccaccacgt ggggccccgt
     2281 ggtcatcacc gccgatgccg ccgggctgct cgcctgccat ctgccggatg ccggcacgcc
     2341 gcgtggcccg ctgcgcaccg gccgcgccac gctgccgccc cacgcgccgc ccgcgctgcg
     2401 gcaggcggtg gccgccgccg ccgcgctgct tgccggccgt actccggcgc agcttccgcc
     2461 gctcagcccc gccgcttggg ctgacgccac cgctttccag cgcgccatct ggcgcgccat
     2521 gcagcgcatc ccgcgcggcc gcaccatgac ctatgccgaa ctcgcccgcc gcgccggacg
     2581 tccgcgcgcc agccgggccg ccggcggcgc atgcggcgcc aaccccttgc cgctctttgt
     2641 gccgtgccac cgtgtcgtcg ccaccggcgg cggcctcggc ggcttctccg gcggcctcgc
     2701 gtggaaggtt cacctcctgc gcgtggaagg cgcacggcca tgaacatcgc cgtcgcttgc
     2761 ggcggcaccg gcgggcacgt tttcccgggg ctggccaccg cgcgagagct gctgagccgc
     2821 ggccacgaag tcaccctctg gctcaccggg cgcgataccg aacaagccgc ccgccaaacg
     2881 tgggatggcc ccgttgtgga agtccccgcc cgtggtctgc cctccggctt ctcgtggcag
     2941 gcgttgcaac gcgccgcgca actggggcag gccatttggc gctgccgcgc ccggatgcgt
     3001 cccgcgcgcc ccgctgtcct gctcgccatg ggctcctatg cctccgtggg cccctgcggc
     3061 gccgcgcggc tgctgggcgt ccccattgtc ctgcacgaag ccaacgtcat ccccggccgc
     3121 gccgtacgcc tcttcgcgcg ccgcgccgcc gccgtcgccg ccggcttcga ggaaacccgc
     3181 taccatctcc cccgcgcccg cctccacgtt gtcggcatgc ccctgcgcga cgaactcacc
     3241 gccgccgccc acgccctgcc gccgcgcccc gccggcgcgc cgttccggtt cctcgtcatg
     3301 ggcggcagca tgggcgcgcg ccgactcaac caagtcgtca ccgccgccct cgcccccctc
     3361 gtcgccgccg gcatcccctt cgccgtcacc cacctcgccg ggcgcgacga cgaacccgcc
     3421 gtccgcgccg cctacgctgc cgccggcatc cccgccgacg tcatcgcctt caccaactac
     3481 atggcccccc tctaccacgc cgccgacttc gccatctgcc gcgccggcgc ttccacctgc
     3541 gccgagcttg ccttcttcgg cgtccccgcc ctgttggtgc cctatcccca cgccgccatg
     3601 gaccaccaaa ccgccaacgc ccgcgccgtc gaaaaacgcg gcgcggccga tgtcgtcgcc
     3661 gaggccgacc tcacccccgc ctggctcgag gcctacctcc tgcaaaccct gcgcaaacct
     3721 gcccgcctcg cccgcctgcg cgatgccgcc ctcaaggccg gcgcccgcaa cggcgctgcc
     3781 gacctcgcca ccctcgtcga gcaaagcgcc cgcccatgac cccccccgcc gaaccttggc
     3841 gcgccgccat ccgccgttgg ctcgcgcagc ccgcccatgt ccacctcctc ggcataggcg
     3901 gcatcggcat ggccggcctc gcccgcctgc ttgccgctcg cggccagcac gtcaccggct
     3961 gcgacgccgc caccccgcgc accctccgct ggctacaagc ccaaggcatc cccgccacca
     4021 ccggccacga ccccgcccat cttgccgacg cccaatgggc catccacagc cccgccgtcc
     4081 ccgccgacca ccccgaaatg ctcgccgccg cccgcctgcc ccttttccgc cgcgggcaag
     4141 tcctccccgt cttggccgag caatggcaaa ccatcgccgt cgccggcacc cacggcaaaa
     4201 ccaccaccgc cgccatgatt acccacatcc tgcgccacac cggcgccgac ccctcctggt
     4261 gcatcggcgg cgaactcgcc cccgaccacg cccccgccgg catcggcacc ggcccatggc
     4321 tcgtcatcga ggccgatgaa agcgacgcca cactcgccca ctacgccccc gccgtcgccc
     4381 tcatcaccaa cattgagttc gaccacatgg agcacttcac cagccccgcc cacttcaccc
     4441 aatgcttccg cgcctacgcc cgccaagccc gccacctcgt ctattgcgcc gatgaccccg
     4501 ccgccgccgc cctcggcgcg gaaatcggcg gcctctccta cggcctcact cccgccgccg
     4561 actaccgcgc cgaaaacccg caacccaccc ccgccggcac ccgcgccacc ctccgcggca
     4621 cgccactcca catccccatt cccggccccc acaacctcct caacgccctc ggtgccattg
     4681 ccgccgtcag ccattgcggc attgacctct ccgccgccgc cgccgccctt gccacctttg
     4741 ccttgccccg ccgccgcttc gagcaaatcg cccacgcccg cggcatccgc atcattgccg
     4801 actacgccca tcaccccgcc gaaatccgcg ccctcatcgc cgccacccgc gagaccggcg
     4861 ccgaccgcat ttgggccatc tttcagcccc accgctacac ccgcacccgc gcccttggtc
     4921 ccgacttccc ccccgccttc gacggcatta ctggcctcat tctgctcccc gtctatgccg
     4981 ccagcgaacc cccattgccc ggcggcaccg ccgccgacct cctcaagcac ctctggcggc
     5041 acaaccaata ccctgccgaa ctcgccgccg acctccccgc cgctgcccac gctgccgccg
     5101 cccgctggca acccggcgat accgtcctcg tcattggcgc cggcgatgtc gaacaaatcg
     5161 ccccccacct ccaagccctc ctccacgcc
//
LOCUS       DAOPNP010000135         5184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_17473_length_5184_cov_8.093780, whole genome shotgun sequence.
ACCESSION   DAOPNP010000135 DAOPNP010000000
VERSION     DAOPNP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17473_length_5184_cov_8.093780"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..796)
                     /gene="trmB"
                     /locus_tag="PKI64_09880"
     CDS             complement(197..796)
                     /gene="trmB"
                     /locus_tag="PKI64_09880"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOE01346.1"
                     /translation="MIADSLRVIPATPAEWLGPLPAASWFAKPDQPLHVDVGCGKGRF
                     LLARAARFPAVNFLGIDRMLRRVRKVDNRARRLDLANIRLLRVEAYYAVAYLLPPAAV
                     AAYYIFFPDPWPKARHEAHRLFNARFLDALHRTLQPGGVVHVATDHWPYFEQLQGVFA
                     ADARFAAVAPFVPEADEQTDFERYYIESKPIGRLSVQKV"
     gene            complement(805..1398)
                     /locus_tag="PKI64_09885"
     CDS             complement(805..1398)
                     /locus_tag="PKI64_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01347.1"
                     /translation="MKKTWLIGLLAVALGAAVHAEEEMDFGAMMKAFSAMSQEGNAAQ
                     PVATVSPKALKELLPKSIPGYERVEAGAEKQGVMGMSTVVARGEYENGAKTLVIEITD
                     LSGLGPFGTMAMVGWAQQEVDKETRGGFERTGSCQGYKSLEKWNKNNQDGEIDVFVAG
                     RFSVKISGSGLDAFEELQPAAKAIDLKALSEVKPETP"
     gene            complement(1454..2329)
                     /locus_tag="PKI64_09890"
     CDS             complement(1454..2329)
                     /locus_tag="PKI64_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOE01348.1"
                     /translation="MKLVRYGAPGAERPGIWQDATKDEPARILDVQGMVFDIADYDAR
                     FWRTFGIARLRALLQEPNLKWVPAAGQRLGPPVAPPRQIICLGKNYRDHVQEFDAQAP
                     ELPVYFAKSPGALCGHGDVIRLRPGMERVDGEVELAVVIGRRARDIQESDALAYVAGY
                     TILNDVTNRDLQKARGQWFFAKSADSFAPLGPWLVTRDEIKRPHSLRIRQRVNGEVLQ
                     DAKTSKMIFRLEMVLADLSRVMTLEPGDVVSTGTPGGIGSARTPPVVLHDGDVMECEI
                     DGIGVLRNPVAMMYG"
     gene            complement(2326..3321)
                     /gene="rnhC"
                     /locus_tag="PKI64_09895"
     CDS             complement(2326..3321)
                     /gene="rnhC"
                     /locus_tag="PKI64_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOE01349.1"
                     /translation="MTEQKNSYTIKLTPEQLAAAAELLATGNYRPRQVPYAVAAAEGD
                     DCQIVLYQSGKCLVQGKGAADWVLFTLEPLVLGEARLGYEEVLNPESITPHMGVDESG
                     KGDFFGPLVIAAAYVDPVIAKDLKAMNVRDSKTITSDKAAQDMAKKIRARLGDKFVVV
                     SIGPAAYNRLYGTMGSVNRILAWGHARAIENLLEKVPGCPRALSDQFGPEQQIQRALQ
                     QKGRKIKLEQRHKAESDIAVAAASVLARSAFLTAMDKLGQQVDVKLPKGASAQVKAAG
                     TEIVKKHGGAKLLEVAKCHFKTTDDILGPLGKTRAGEKLPPAPERKEYVPRRPAK"
     gene            3455..3760
                     /locus_tag="PKI64_09900"
     CDS             3455..3760
                     /locus_tag="PKI64_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOE01350.1"
                     /translation="MKNFFALILAASLLALAAGCAALSSGDWAADPTSDAGITALANS
                     RLNNDFVVGRATLSASVENGMATLYGAVPDEATRQRALQILRGTEGVFDVIDRTRMR"
     gene            3785..5041
                     /locus_tag="PKI64_09905"
     CDS             3785..5041
                     /locus_tag="PKI64_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE01351.1"
                     /translation="MRTRRPPCRRPSWPAGILAAALILGAGCATTLPSATTNAYRRAL
                     RSIPEVPCNQLRADRAILAAFFAANNVPLSPAEIAQIIPPANPPGLLDRHALKKIATQ
                     KKRVLLVVKADERFLWDELGHNLPLLVLLPDGIKYQPTAPAFIPIAWDQKARVIELLD
                     GNGEIQHIAETSFFTRRDPLRQAALCLTTPGHMSHFQPTREQKLVLADFWYDSGFYRR
                     ATATYTAVARAAPTDTTDVAALLGQGNTLVQQHRYDDAIPIFRAALAADPDNPRVLNN
                     LAYCHLQTGTQLMTALRHANKAVQLDPTNPIILETLGSLNLKIGDAATAARHLEHAWA
                     HARKRSPEIQIAIMDQLVRAWLASGRNDLAWQVAEHRHRAFPEYKMPADILSNFPALR
                     QRAVPYPESKPAPTPKPIKATPAPKK"
BASE COUNT          958 a         1923 c         1358 g          945 t
ORIGIN      
        1 ggcccccata gaagggctgg aacggtcgca gaccttccag ccgctcccgg cgggagaagg
       61 tacagtcccc caaccattcc cgcccacaaa agcccggcga aaccagcgaa atggttgcgt
      121 gtccccatgt gaaattcggg gctggccatg cagggcgaag cgcggacctg atcctcccgc
      181 cccgctccac cccgcgtcaa accttttgca ccgagagccg gccaatgggc ttggactcga
      241 tgtaataccg ctcaaagtcg gtttgctcgt cggcctccgg cacaaacggc gccaccgcgg
      301 caaaccgcgc atccgccgca aacacgcctt gcagttgctc aaaataaggc caatggtcgg
      361 tggccacatg caccacgcca ccgggttgca gcgtgcggtg caacgcatcc agaaaacgcg
      421 cattgaacaa ccggtgtgcc tcgtgacgcg ccttgggcca cgggtcgggg aaaaaaatgt
      481 agtaggcggc taccgcggcc ggcggcagca gataggctac tgcgtaatag gcctccaccc
      541 gcagcaaacg gatattcgcc aagtccagcc ggcgcgcgcg gttgtccacc ttgcgcaccc
      601 gccgaagcat ccggtcaatg cccaaaaagt tcaccgccgg aaatcgtgcg gcgcgcgcca
      661 gcaagaagcg ccccttgccg caccccacat ccacgtgcaa tggctggtcc ggcttggcaa
      721 accacgaggc cgccggcaac ggccccaacc attcggcggg cgttgccggg attacgcgca
      781 ggctgtccgc aatcacaaaa cccgctacgg ggtttccggc ttcacctcgg acagggcctt
      841 gagatcaatg gccttggcag ccggctgcag ctcttcaaac gcatcaaggc cggagccgga
      901 aatcttcacg ctgaagcggc ccgccacaaa gacgtcaatt tcgccgtctt ggttgttctt
      961 gttccacttt tcgaggctct tgtaaccctg gcagctcccg gtgcgttcaa agccgccgcg
     1021 cgtttcctta tccacttcct gctgtgccca gcccaccatg gccatggtgc cgaacggccc
     1081 cagcccgctc agatcggtaa tttcaataac caaggttttg gcgccatttt catactcgcc
     1141 gcgggccacc accgtgctca tgcccatcac gccctgcttc tcggcgccgg cttccacccg
     1201 ctcatagccg gggatggact tgggcaacag ctccttgagc gccttgggcg aaaccgtcgc
     1261 caccggctgg gccgcattgc cctcttgcga cattgcgctg aatgctttca tcatcgcgcc
     1321 aaaatccatt tcttcctcgg cgtgtaccgc cgcgcccagg gccaccgcca gcaaaccaat
     1381 cagccatgtc ttcttcatcg tgagggtcct tttgttgttt gtcgcccggc gatttcgttc
     1441 gccgcggcag ttttcaacca tacatcatcg ccacgggatt gcgcaacacg ccgatgccat
     1501 caatttcgca ttccatcaca tcgccatcgt gcagcaccac gggcggcgtg cgcgccgacc
     1561 cgattccgcc cggcgtgccg gtggaaacca catcgcccgg ctccagcgtc atcacgcggc
     1621 tcaaatcggc cagcaccatt tcgaggcgga aaatcatctt ggatgttttg gcgtcttgca
     1681 gcacttcgcc attcacccgc tggcgaatcc gcagcgaatg gggccgcttg atttcgtcgc
     1741 gcgtcaccag ccacggcccc agcggcgcaa agctgtccgc gctcttggca aaaaaccatt
     1801 gcccgcgcgc cttctgcaaa tcgcggttgg tgacgtcgtt gaggatggtg tagccggcca
     1861 cataggcgag cgcgtcgctt tcctgaatat cacgcgcgcg gcggccgatc accacggcca
     1921 actcgacctc gccatccacc cgttccatgc cggggcgcag gcggatcaca tcgccatggc
     1981 cacacagcgc gccggggctc ttcgcaaaat acaccggcaa ctcgggcgcc tgcgcatcga
     2041 actcctgcac gtggtcgcgg tagtttttgc cgaggcaaat gatctgccgt ggcggggcca
     2101 ccggcggccc gagccgctgc ccggcggcgg gcacccactt caagttgggc tcttgcagca
     2161 gtgcgcgcaa ccgcgcaatc ccgaatgttc gccaaaaccg cgcgtcataa tcggcaatat
     2221 caaacaccat gccctgcaca tccagaatcc gggcgggctc atccttggtg gcgtcctgcc
     2281 aaatgcccgg tcgttcggcg ccgggcgcgc cgtagcggac caacttcatt tggcgggcct
     2341 ccgcggcaca tactctttgc gctccggcgc gggcggcaat ttttcgccag cgcgggtttt
     2401 gcccagcggc cccaaaatgt cgtccgtggt tttgaagtgg cacttcgcca cctccagcaa
     2461 tttcgcgccg ccgtgttttt tgacgatctc cgtacccgcc gccttcacct gcgccgaggc
     2521 gcccttcggc agcttgacgt ccacctgttg ccccagcttg tccatcgccg tcagaaatgc
     2581 ggaacgcgcc aacaccgaag ccgctgccac cgcgatgtcg ctctccgcct tgtgccgttg
     2641 ctccagcttg atcttgcgcc ctttttgctg caacgcgcgc tggatttgct gctcggggcc
     2701 gaactggtcg ctcaacgccc gcgggcatcc cggcactttt tccagcagat tttcgatggc
     2761 ccgcgcgtgg ccccatgcca gaatccggtt cacgctgccc atcgtgccat agagccggtt
     2821 ataggccgcc ggcccgatgc tcaccaccac aaacttgtcg cccagccgcg cgcggatttt
     2881 cttggccata tcttgcgcgg ccttgtcgct ggtgatggtt ttgctgtcgc gcacattcat
     2941 ggccttgagg tccttggcaa tgaccgggtc aacataggcc gcggcaatca ccagcggccc
     3001 gaagaaatcg cccttgccac tttcgtccac gcccatgtgc ggcgtaatgc tctccgggtt
     3061 cagcacctct tcatagccga ggcgcgcctc gcccagcacc agcggctcca acgtaaacag
     3121 cacccaatcc gccgcgcctt tgccctgcac cagacacttg ccgctttggt agagcacaat
     3181 ctggcagtca tcgccctcgg ccgccgccac cgcatacggc acctgccgcg gccggtagtt
     3241 gccggtggcc agcagctccg ctgccgccgc caactgctcc ggcgtcagct tgatggtgta
     3301 ggaattcttt tgttccgtca tgattgggca acctaacaaa cccgcccccc gctggcaacc
     3361 gcgcgcgacg ttgacattcc cccactggca gcataaggtt tttttgtgtc aaagcgtggc
     3421 ccgccacacg gccccacacc accggagagc caacatgaaa aacttttttg cgctgattct
     3481 cgccgccagc ctgcttgcgc tggccgccgg gtgcgccgcc ctctcaagcg gcgactgggc
     3541 cgccgacccc accagcgatg ccggcatcac cgccttggcc aatagccggc tcaacaacga
     3601 ctttgtcgtt ggccgcgcca cactcagcgc cagcgtcgaa aacggcatgg ccaccctcta
     3661 cggcgcggtg cccgacgaag ccacccgcca acgcgccctc caaatcctgc ggggcaccga
     3721 aggcgtcttt gacgtcattg accgcacacg gatgcgctaa gcgtccccgc cggagtccgg
     3781 acccgtgcgg acgcgccgcc caccttgccg ccggccctca tggcccgccg gcatcttggc
     3841 tgcggcgctc atccttggcg ccggttgcgc caccaccctg cccagtgcca cgaccaacgc
     3901 ctaccgccgc gccctgcgct ccattcccga agtcccctgc aaccagctcc gtgccgaccg
     3961 cgccattctg gccgccttct ttgccgccaa caacgtcccg ctctctcccg ccgaaattgc
     4021 gcaaatcatt cccccggcca atccacccgg cttgctcgac cgccacgccc tgaaaaaaat
     4081 cgccacgcaa aaaaaacgcg tcttgctcgt ggtcaaagcc gacgaacgct tcctatggga
     4141 tgaactcggc cacaacctgc cgctgctggt cctcctgccc gacggcatca aataccagcc
     4201 cactgccccc gcctttatcc ccatcgcgtg ggatcaaaag gcgcgcgtca ttgaactcct
     4261 cgacggcaac ggcgaaatcc agcacatcgc ggaaacttcc ttcttcaccc ggcgcgaccc
     4321 cttgcgacag gccgcgcttt gccttaccac ccccggccac atgtcgcact tccagcccac
     4381 ccgcgagcag aaactggtcc tcgccgactt ctggtacgac agcggcttct accgccgcgc
     4441 caccgccacc tataccgccg tggcccgggc cgcccccacc gacaccaccg atgtcgccgc
     4501 gctcctcggg cagggcaata ccctcgtcca gcagcaccgc tacgacgacg ccattcccat
     4561 cttccgtgcc gccctcgccg ccgaccccga caacccgcgc gtcctcaaca acctcgccta
     4621 ctgccatctg caaaccggca cgcagctcat gaccgccctg cgccacgcca acaaagccgt
     4681 gcagctcgac cccaccaacc ccatcattct ggaaaccctc ggcagcctga atttgaaaat
     4741 tggcgacgcc gccaccgccg cccgtcattt ggagcacgct tgggcccacg cccgcaagcg
     4801 ctctccggaa atccagattg ccatcatgga tcaactggtc cgcgcttggc tggccagtgg
     4861 ccgcaacgac ctcgcctggc aagtcgccga acatcgtcac cgtgccttcc ccgagtacaa
     4921 aatgccggcg gatatcctga gcaactttcc cgccctacgc cagcgagccg ttccctatcc
     4981 ggagtccaaa cccgccccca cccccaagcc catcaaagcc acccccgccc ccaaaaaata
     5041 aaaaaaagtc actatcatcc catagaagca ggttatgaag cattgacctg ctaaattctt
     5101 ggtaaattcg cggggtctgg cgcgatttct attaattcgg taattatata gttatacagc
     5161 cgcgtagcgt acgcaggggt ggcc
//
LOCUS       DAOPNP010000136         5181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_17496_length_5181_cov_10.357199, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000136 DAOPNP010000000
VERSION     DAOPNP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17496_length_5181_cov_10.357199"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            942..2768
                     /gene="typA"
                     /locus_tag="PKI64_09910"
     CDS             942..2768
                     /gene="typA"
                     /locus_tag="PKI64_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOE01352.1"
                     /translation="MNSAPPIRNVAIIAHVDHGKTTLVDAILRQLHVAGGDAAAQPCI
                     LDNNALERERGITILAKNVSVRHAGVKINLIDTPGHADFGGQVERVLNLADGVLLLVD
                     AAEGPMPQTRFVLAKALQLNLKPIVVLNKMDKPAAQPGATLDAIFDLFVELGATHEQL
                     DFPVLYAVGRDGWAVRDLAQEPRTTIVPLLDAILAHIPPPARVAGPVQMQITTLDYSD
                     YTGRIGIGRVRRGTLALGQPLALVKQDRRVLPGQPRALYTFEGLGQAPATTVPCGDIC
                     AVHGVDEVDIGDTLTPVDCPEPLPPLALDAPTIAMLFRINDSPGFGATGTYLTSRHIR
                     ERLHRETQRDVALAFTEVGEGSFQVSGRGVLHLAILIENMRREGYELTISRPRVIVKT
                     LHGVRHEPVEILVVDTPDAATGPVIENVGKRQGQMQRMHAGGGRTLLEFVVPTRGLIG
                     LRSKLLTATRGEAIISHRFLRYEPMHDDIPQRLNGALISMEQGRANLYALDGLQDRGC
                     FFVAPGDPCYEGQIVGEHCKDSDLVVNIQRAKKLTNIRAAGADRKLVVAPPVRLSLEE
                     ALEYINDDELVEATPAAIRLRKYFLTEVERKRHRNHDWTQAG"
     gene            2834..3586
                     /gene="radC"
                     /locus_tag="PKI64_09915"
     CDS             2834..3586
                     /gene="radC"
                     /locus_tag="PKI64_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOE01353.1"
                     /translation="MKDAPPPASPAYQISLSRIKDLPQHRRPREIAREAGIQNVPDDV
                     LVAILLRAGIRGQSVIDLARQVLDRHQGSLTRLAEASLPELRAIKGIGAVKAITLSAA
                     LELGRRAAAETTAPLPLIREPAHVLTLLQNDTARLDQEVFWVLILDQKYRLRRAPEPI
                     TKGTLNASFSHPRDVFREAIRLSAAAIILAHNHPSGDPTPSAEDIRSTRQLVSTGQVV
                     GIQVLDHIIIGGHRPHPAPYISLREAGLVDFS"
     gene            4261..4686
                     /locus_tag="PKI64_09920"
     CDS             4261..4686
                     /locus_tag="PKI64_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01354.1"
                     /translation="MRKTKVARQRLLPHPQRDAPFTENNDAQQKFHTVEKSFLIPPPV
                     QRRPTSNAPAAFLGRGCNLRRAVICPGLSEAGYRKSLSPTKHLLLPFAVLASWRDSSS
                     CPNQAQSTKNQAPPAVFLLVFFDPAAGSSGACHREKESV"
     gene            4734..>5181
                     /locus_tag="PKI64_09925"
     CDS             4734..>5181
                     /locus_tag="PKI64_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE01355.1"
                     /translation="MKKVFFSVLISLLFLTLPAFAKIEAAVRPGPLVVGSPAPELKAA
                     QWIKGEEVRGFDPERTYVVEFWATWCPPCRASIPHLTALAHQFPQVAFIGMNVWERGD
                     NAAETVTAFVKKMDEQMDYSVAMDTSDAFMAKHWMEAAEQRGIPTAF"
BASE COUNT         1154 a         1799 c         1323 g          905 t
ORIGIN      
        1 tgggccatga tgccgatgtt ccgcacgtgc gaaaggggag ttgttctagc catgtaatct
       61 gcctgtctgt ccgatataga gacccccgat gtctggcaac accgggggcg ctcactcaaa
      121 acgggcgcgc atcatgggct gcgcctgcgg cttgtgcaag aaaaaatacg cgcttgcgcc
      181 agcttggatc attcacgcga caagtctgcc caaaatgcaa ggcgggcggg cgaagctggc
      241 tgggcattct gcgcgccccg cccaacactg ccgctggggc ggaagcgtcg caccgcaggg
      301 caacagggat gaaattaaat ttgttctatc aaaaatccgc ctggctcatc ggcgcaatcc
      361 gtaacggcgt tttcagccat ggtgcttaat gattcaggct agagcattta atgtagccgg
      421 gaggtcgtcg cgaattggga ttggccaagg aaaacgaagc aggaatagca gcgctatttc
      481 gatgcagcct gacgacggcc aagcccaatg cagtagacaa aacgggacat tcgccgattc
      541 tagtgggggt cttgggatcg gcgccttcca tgcgggaccg aaccgcgcgg ataaaaacgc
      601 aaaaatcact cacccaaaaa atgcccagag tttataaagt gtggacagca ccctgaaatt
      661 ggctgattaa aaatagccaa taaaacagac ataacaaatt gtatatgatt gagatagaat
      721 tattacaaca tttgcccaga gtttataaac tctggacagt atggttaaaa tgagaaaaac
      781 gaaggtggcc tgacaacggc tgcttccaca tccccagcga gacgatccat ccaccaaaaa
      841 cagcgatgaa ccacaaaacg gctacatcat tgatgaaaat gctctaaaca gttgtcaaat
      901 ggccgcgttg ccgtatagtc ccgcctccgc acacccaccg catgaactcc gccccgccca
      961 ttcgcaacgt tgccatcatc gcccacgtgg atcacggcaa gaccacgctg gtggatgcca
     1021 tcctgcgcca gctccacgtg gccgggggcg atgcggccgc gcaaccgtgc atcctcgaca
     1081 acaacgcact cgagcgcgag cgcggcatca ccatcctcgc caaaaatgtt tccgtgcgcc
     1141 acgcgggggt caagatcaac ctcattgaca cccccggcca cgcggacttc ggcgggcagg
     1201 tggagcgcgt cctgaatctg gccgatggcg tcctgctgct ggtggatgcc gccgaaggcc
     1261 ccatgccgca aacccgcttt gtgctggcca aggcgctgca gctcaacctc aagcccatcg
     1321 tggtgctgaa caaaatggac aagccggcgg cgcaacccgg cgccacgctc gatgccatct
     1381 tcgacctgtt cgtggaactc ggcgccaccc acgaacaact ggattttccc gtgctctacg
     1441 ccgtcgggcg cgatggctgg gccgtgcgcg atctcgccca ggagccgcgc accaccatcg
     1501 tgccgttgct cgacgccatc ctcgcgcaca tccccccgcc cgcccgggtg gccggccccg
     1561 tgcagatgca aatcaccacg ctcgactatt cggattatac ggggcgtatc ggcattgggc
     1621 gcgtccggcg cggcaccttg gcgctgggcc agccgctggc cctcgtgaag caggatcgcc
     1681 gcgtgctgcc cggccagccc cgcgcactct acaccttcga gggccttggc caggcccccg
     1741 ccaccaccgt gccctgcggc gatatctgcg ccgtccacgg cgtggacgag gtggacatcg
     1801 gcgacaccct cacgcctgtg gactgccccg aacccctgcc cccgctcgcg ctcgatgctc
     1861 ccaccatcgc catgctcttt cgcatcaacg actcccccgg cttcggcgcc accggcacct
     1921 acctcacctc ccgccacatc cgcgaacgcc tccaccgcga aacccagcgc gatgtcgccc
     1981 tcgccttcac ggaagtcggt gaaggctcct ttcaagtcag cgggcgcggc gtgctacatc
     2041 tggccatcct catcgaaaac atgcgccgcg aaggctacga gctcaccatc tcccgcccgc
     2101 gcgtcatcgt caaaaccctg cacggcgtgc gccacgagcc ggtggaaatc ctcgtggtgg
     2161 acacccccga tgccgccacc gggcccgtca ttgaaaacgt tggcaaacgc cagggccaaa
     2221 tgcagcgcat gcacgccggc ggcgggcgca ccctgctgga atttgtggtg cccacccgcg
     2281 gcctgatcgg cttgcgcagc aaactcctca ccgccacccg cggcgaagcc atcatcagcc
     2341 accgcttcct gcgctacgaa cccatgcacg acgacatccc ccagcgcctc aacggcgccc
     2401 tcatcagcat ggaacagggc cgcgccaacc tctacgccct cgatggcctc caagaccgcg
     2461 gctgtttctt cgtcgccccc ggcgacccct gctacgaagg ccaaatcgtc ggcgaacact
     2521 gcaaagactc cgacctcgtc gtcaacatcc agcgcgccaa aaaactcacc aacatccgcg
     2581 ccgccggcgc cgaccgcaaa ctcgtcgtgg ccccacccgt gcgcctctcc ctcgaagaag
     2641 ccctcgaata catcaacgat gacgaactcg ttgaagccac ccccgccgcc atccggctgc
     2701 gcaaatattt tttgaccgaa gtcgaacgca agcgccaccg gaaccacgac tggacccaag
     2761 ccggctgacc cgccccgcgc cgcgcgaaaa aaataactgt tcaagccccc ggcccacccg
     2821 ttatcgtgcg cccatgaaag acgcgccgcc ccccgccagc cccgcctacc aaatctccct
     2881 ctcgcgcatc aaagacctcc cccagcaccg ccgcccccgc gagatcgccc gcgaagccgg
     2941 catccagaac gtccccgacg atgtcctcgt ggccatcctc ctgcgcgccg gcatccgcgg
     3001 ccaaagcgtc attgacctcg cccggcaggt gctcgaccgc caccaaggct ccctcacccg
     3061 cctcgccgaa gcctccctcc ccgaactgcg cgccatcaaa ggcatcggcg ccgtcaaagc
     3121 catcaccctc agcgccgccc ttgaactcgg ccgccgcgcc gccgccgaaa ccaccgcccc
     3181 gctccccctc atccgcgaac ccgctcacgt cctgaccctg ctgcaaaacg acaccgcccg
     3241 cctcgaccag gaagtcttct gggtcctcat cctcgaccaa aaatatcgcc tgcgccgcgc
     3301 ccccgaaccc atcaccaaag gtaccctcaa cgccagcttc tcccacccgc gcgacgtttt
     3361 tcgcgaagcc atccgcctct ccgccgctgc cattatcctc gcccacaacc acccctccgg
     3421 cgaccccacc ccctccgccg aagacatccg cagcacccgc caactcgtca gcaccggcca
     3481 agtcgtcggc attcaggtat tggaccacat catcatcggc ggccaccgcc cccaccccgc
     3541 cccctacatc agcctccgcg aagccggcct cgtggatttc tcctgacccc accgcacttt
     3601 tccacaccgt ggaaaaaagt ttatcccgcg tgagcgggat tttccacact gtggaaaata
     3661 gacgagtgcc gagcccaagt ccgcaggacg ccgggtgaac gcgagttcta tagcggcggc
     3721 gcagccgtcc gagcaaacga gccatagcga gcgtagcggc aaatccgcgg caaaaattcc
     3781 acactgtgga aaaattcacg ccctgccgcc ctattttttc cacacaatgg aacaactttg
     3841 agtccacttt tccacgcaat ggaacaactt tgggcaaaag ttccacaccg tggaaaatag
     3901 acgagtgccg agcccaagtc cgcaggacgc cgggtgaacg cgagttctat tgcggcggcg
     3961 cagccgtccg agcaaacgag ccatagcgag cgtagcggca aatcagcagc aaaagggaca
     4021 ttcgccgatt ttagtgagtg gcttaggatc ggcgccttcc atgcgggacc gcgccgtgcg
     4081 actaaaaacg caaaaatcac tcaccaaaaa aatgcccaga gtttataaag tgtggacagc
     4141 accctaaaat tggctgatta aaaatagcca ataaaacaga cataacaaat tgtatatgat
     4201 tgagatagaa ttattacaac atttgcccag agtttataaa ctctggacag tatggttaaa
     4261 atgagaaaaa cgaaggtggc cagacaacgg ctgcttccac atccccagcg agacgctcca
     4321 ttcactgaaa acaacgatgc ccagcaaaag ttccacactg tggaaaaaag ttttttgatc
     4381 ccgccgccgg tacaacggcg gccgacatca aacgcgcctg ccgcttttct tggccgggga
     4441 tgcaatctcc ggcgggctgt tatttgcccc ggcctcagcg aggccggcta cagaaaatcc
     4501 ctttccccaa ccaagcacct ccttctcccc ttcgcggtct tggcgtcttg gcgagattca
     4561 tcttcctgcc ccaaccaagc acaaagcaca aagaaccaag cacctccggc tgttttcctg
     4621 ctcgtttttt ttgatcccgc cgccgggagt tccggtgctt gccatcgtga aaaagaatcg
     4681 gtgtaatgcg gaggcatggg tactctcagc catggcgcgg aaagggtgga ggcatgaaaa
     4741 aagttttttt ctcggttctg atttcattgt tatttctcac cttgccggcc ttcgccaaaa
     4801 tcgaggccgc tgttcgcccc ggcccgctgg ttgtcggctc tccagcgccg gaattaaaag
     4861 cagcgcagtg gatcaaaggc gaggaagtgc ggggctttga tccggagcgc acttatgtgg
     4921 tcgaattttg ggccacctgg tgtccgccct gccgcgcatc catcccccac ctgacggctc
     4981 ttgcgcacca atttccccag gtggcgttta tcggcatgaa cgtctgggaa cgcggcgaca
     5041 atgcggcaga aacggtcact gcttttgtca aaaaaatgga cgagcaaatg gattactccg
     5101 ttgcgatgga cacttcggac gcgttcatgg cgaaacactg gatggaggcc gccgagcaac
     5161 gcggcatccc cacggctttt g
//
LOCUS       DAOPNP010000137         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_17882_length_5089_cov_8.066547, whole genome shotgun sequence.
ACCESSION   DAOPNP010000137 DAOPNP010000000
VERSION     DAOPNP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17882_length_5089_cov_8.066547"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKI64_09930"
     CDS             complement(<1..197)
                     /locus_tag="PKI64_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01356.1"
                     /translation="MAACPATNPIPSHHAKLFISASVETVASSILMGNHVKQYIVRFS
                     FSFVRKNGGHAKLIVEESASES"
     gene            196..1845
                     /locus_tag="PKI64_09935"
     CDS             196..1845
                     /locus_tag="PKI64_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01357.1"
                     /translation="MAWAFPSWMGVYGSFSTHNGSNPGVYTILMNQDYYGLHAEVGIG
                     ISNHWTTQAMTYMGNADGNSLWQVHPGVALPAGAAVEYYFHGWDDWGGHIYANNNGSN
                     YSFSASPAELEWIGATEHTPASPAAGQDIRIWTQTWPPGAGQSGFVLYAADSQWSGAG
                     LSKTATTNQNDLWSGILGRFLPGVELEYVVGVEDGAETTHYDNNAGSNYSVSVSTGAP
                     ITYLGGAYHWPLNGVLSSSDSLWLNLFVAPSQTLVNAAAEYSVNGWIWERARLDFWQM
                     DGTNEWWHIELGQMPPASTVWYAFDAQDGAGASHIRPTNGLPYSATVAGSSTDSDADG
                     LPDDWESFWFGGLTHTTASDNPDGDGLPDMPMDNWMEYVMGTDPAVSNAIEEIAVLWK
                     PSLPLQGGALMISAASEAFAGLGLSGITAHFSDGGSATLYAVTGGRFQGAALLSETST
                     VCKITSLSGGGQTDDNRGIGWTIPIQPLGGGEPADSDGDGMPDAWELANGLNPFANDA
                     DGDADGDGISNLQEYLHGLNPQVADPWPEIVLLWPQDGQEI"
     gene            1856..2368
                     /locus_tag="PKI64_09940"
     CDS             1856..2368
                     /locus_tag="PKI64_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01358.1"
                     /translation="MKQVAVGLVWLALGVAWANAGYESISPPGERGRQRMTTSETASG
                     IVNRAAWTGGIVLADGTEIKLENAGGHFLPVRLESGIQCRMWVAGPGLVPGRSVVLFT
                     RCGGFINGSVKDDVTVGADGCLRFDYQNGVFGSQPIQATLMGNTATLLTVNATKNSQD
                     PPEEEAHETK"
     gene            2355..>5089
                     /locus_tag="PKI64_09945"
     CDS             2355..>5089
                     /locus_tag="PKI64_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01359.1"
                     /translation="MKPNKKFLFWALVMGLLVAMMAMPAFGEEEDCDNEDPPECDPCE
                     EECDDSDGTNRFEVYSGNVKRSVSDLKMAVQVGEHPLVFSRMHSSRSVWAVQVRSDFV
                     FGRAGNWRHSHQWTILDDGEFNGCQGLQVIAPNGRVNYYHKKTTNDLFMTYLPGTHSR
                     IWPDGSTNFYLYHLDGTKYHITQGGGVSNKTFRMEGFWDAYSNWYGYAYGEDGYLVRV
                     TGPNTNQYLELEYRDLEGAVAPGWVRFSYTNEQAAEVLIAGTWNDWTGTQTPMQTNSS
                     GIWEADLELGEGFYEYKFAVRNQGGGAWQLVADPDNPLVVGPDSNSLAVVSAERIIAR
                     VSAGDGRSVEYVYDWDWNNGQKVLAMRLREAQYGTGESALYDYYPAAHDQYRGMLLKS
                     ADDPHVAGAARAIFYTYHTNRLYSGQIHEERLLSSGQLVARLEYDPTNHLRRTVVKAD
                     GSTNEYLFVSGGANTEQKTDAAGQTIRRIHFGGNGMLQARVDPMGRTSVYTRTWHFGA
                     VLSVSNACGCRGDRVNTYTDNTYPFYLASTADAEGNTTMYQRDSAHRPIAVYHPDGTS
                     EYFEYNAHGQATVEKRRDGTTWTNAYDLRGRRTSTTGPGGACTGYGYDEHDRVSSETN
                     ALGLVTTTEYNWAGKPTRKVYPDSTEEVWQYDRYGSVTQAVSRSGGITVYEYDERGNQ
                     IRVTDPVGAVTETTYDLDGRKVLETSPSGLVVSNTYDVLGRRIRRTYSSDNTFEAWTY
                     VYDGVSTHTNRLGYGTTNAYNADGQLASVTDTRGNTTHYTYDQLGRRVAIQNALGQTT
                     TQTLDPSGRFTSTTDDEGLVVTNTYNANGQWIRSVQRGGTTNDYAYDTAGRRTHVVLN
                     GATVSSTAYDAIGRTVWTRNADGVVVSNAYDSAGRLYRVYMPDGTFTENVYSNGWL"
BASE COUNT         1110 a         1508 c         1542 g          929 t
ORIGIN      
        1 gactcgcttg cggattcctc gacaatcaat ttggcgtgtc cgccattttt ccgaacaaaa
       61 ctgaatgaaa aacgaacaat atactgtttg acatggttgc ccattaggat tgaactggcg
      121 acagtttcaa cggaggcgga tatgaaaagc ttcgcatggt ggcttgggat tgggttggtt
      181 gcggggcatg cggccatggc ctgggcgttc ccaagctgga tgggggttta tggcagcttt
      241 tccacccaca atggctcgaa tccgggtgtc tataccattc tgatgaatca ggactattac
      301 gggcttcatg cggaggtcgg cattggcatc agcaaccact ggacgaccca ggcaatgacc
      361 tatatgggca atgccgacgg caactcgctg tggcaggttc atccgggtgt cgcgttgccc
      421 gccggcgccg ccgtggagta ttactttcat ggctgggacg actggggtgg ccatatctac
      481 gcgaacaaca atggttccaa ctacagtttc agcgccagcc cggcggaact ggaatggatc
      541 ggagcgacgg aacacacgcc cgcctctccc gccgccggac aagacatccg aatatggacg
      601 cagacctggc cgccaggcgc cgggcaaagc gggttcgttt tgtatgcggc agattcgcag
      661 tggtcgggcg ccgggctgag caagacagcc accaccaacc agaatgatct ttggagcggc
      721 atcttggggc ggtttctgcc gggggtggaa ttggaatatg tggtgggagt ggaagacggc
      781 gctgaaacca cccactatga caacaatgca ggcagtaatt attccgtatc ggtctcgacc
      841 ggcgccccca tcacgtatct gggtggcgcc taccattggc cgctcaatgg cgtattgagt
      901 tcgtctgaca gcctgtggct caacctgttt gtcgctccgt cgcaaaccct ggtgaacgct
      961 gcggccgaat acagcgtgaa tggttggatt tgggaacgcg cccggctgga cttctggcag
     1021 atggacggca ccaacgaatg gtggcacatc gaactcggcc agatgccgcc ggccagcacg
     1081 gtgtggtacg cctttgacgc gcaagacggc gcgggcgctt cgcacatccg cccgaccaac
     1141 ggcctgccct attccgccac cgtcgccggc agctccacgg acagcgatgc cgacggcctg
     1201 ccggacgatt gggaatcctt ctggttcggg gggctgaccc acacgaccgc cagcgacaac
     1261 ccggatggcg acggcctgcc ggacatgccg atggacaact ggatggaata tgtcatgggc
     1321 acggaccccg ccgtctccaa tgccatcgaa gaaatcgcgg tgttatggaa accctccctc
     1381 ccgctgcaag gcggcgcgtt gatgatctcg gccgcatcgg aagccttcgc cgggctgggg
     1441 ttgtccggca tcacggccca cttctccgat ggcggcagcg ccacgcttta cgcagtgacc
     1501 ggcggccggt tccaaggcgc cgccctgctt tcggaaacca gcacggtctg caaaatcacc
     1561 tccctgtccg gcgggggaca gaccgacgac aaccgcggaa tcggctggac catccccatc
     1621 caaccgcttg gcggcgggga acccgccgac tcggacggcg acggcatgcc cgatgcctgg
     1681 gaactggcca acgggttgaa tcccttcgcc aacgatgccg atggcgatgc ggacggggac
     1741 ggaatctcca acctgcagga gtatctgcat ggactgaacc cgcaggtggc cgatccctgg
     1801 ccggaaatcg tgctcttgtg gccgcaggac gggcaggaaa tctaaggagg gtgcaatgaa
     1861 acaagtagcc gtgggtttgg tatggctggc actgggagtc gcttgggcga atgcgggata
     1921 tgaaagcata tccccgcccg gagagcgagg ccggcaacgc atgacaacaa gcgaaaccgc
     1981 atccgggatc gtcaatcgcg ccgcctggac cggtgggatc gtgctggctg atggcacgga
     2041 aatcaagttg gaaaacgcgg gcggtcattt cctgccggtc aggctggagt ccggcattca
     2101 atgccggatg tgggttgcgg gaccgggatt ggttcccggt cgctccgtgg ttctgttcac
     2161 caggtgcggc gggttcatca atggcagcgt gaaagatgac gtgacggtag gggcagacgg
     2221 ctgcctccgt ttcgactatc aaaacggcgt gttcgggtcg cagcccatcc aagccactct
     2281 gatgggcaat accgccactc tactgacagt caacgcgaca aagaacagcc aggacccgcc
     2341 ggaagaggaa gcccatgaaa ccaaataaga aattcctgtt ctgggcgttg gtgatggggt
     2401 tgttggtggc catgatggcc atgccggcat ttggtgaaga agaggattgc gacaacgaag
     2461 acccgccgga gtgcgatcct tgtgaagaag aatgcgacga cagcgatggc accaaccggt
     2521 ttgaagtgta ttccggcaac gtcaagcgct cggtgtcgga tttgaaaatg gcggttcagg
     2581 tcggcgaaca cccgctggtg ttttcccgca tgcattcgag ccgctccgtc tgggcggtgc
     2641 aagtccgatc agacttcgtt ttcggacggg cgggaaactg gcggcatagc catcagtgga
     2701 ccattctgga cgatggtgag ttcaatgggt gtcagggatt gcaggtcatc gcccccaatg
     2761 gccgggtcaa ctactatcat aaaaaaacaa cgaatgacct gttcatgacc tatctgccgg
     2821 gcactcactc ccggatttgg ccggacggct cgaccaactt ctatctctat catctggatg
     2881 gaaccaaata ccacattacc caaggcggcg gtgtgtccaa caaaacgttc cggatggaag
     2941 gcttttggga tgcctattcc aactggtatg ggtatgccta cggggaagac ggatatctgg
     3001 tccgcgtgac aggacccaac accaaccagt atctggagct ggaatatcgc gacctggaag
     3061 gtgccgttgc cccgggctgg gttcgcttca gctatacgaa tgagcaagcc gccgaagtgc
     3121 tgatcgccgg cacatggaat gattggacgg gcacccaaac ccccatgcag accaacagta
     3181 gcggcatctg ggaagccgac ctggaactgg gcgaaggctt ctatgaatac aaattcgcgg
     3241 tgagaaacca aggcggcgga gcgtggcaac tggtcgcgga tccggacaac ccgctggtgg
     3301 tcggccccga ctccaattcc ctggcggtgg tcagcgccga acgaatcatc gcgcgcgtgt
     3361 cggccgggga cggccgcagc gtggagtatg tctatgactg ggactggaac aatgggcaga
     3421 aggtgttggc catgcgcctg cgcgaggcgc aatacggcac cggcgagtcc gccctgtacg
     3481 actattatcc cgccgcgcac gaccagtacc gcggcatgct gttgaaatcg gcggacgatc
     3541 cccatgtcgc cggagccgcc cgcgccatct tttataccta ccacaccaac cggctctatt
     3601 ccggacaaat tcacgaagag cggcttcttt ccagcggtca gctggtggcg cggctcgaat
     3661 acgaccccac caaccacctg cgccgaacgg tcgtgaaggc cgatggctcc accaacgaat
     3721 atctgttcgt gtcgggcggg gcgaacacgg agcaaaaaac cgacgccgcc ggacagacca
     3781 tccggcgcat tcactttggc ggaaacggca tgttgcaggc ccgggtggac ccgatggggc
     3841 gcacctctgt ctacacccgc acctggcatt tcggcgcggt gttgtcggtc tccaacgcct
     3901 gcggttgccg gggagatcgc gtcaatacct ataccgacaa cacctatcca ttctacttgg
     3961 catccacggc ggatgccgag ggaaacacca ccatgtatca gcgggattcc gcgcatcggc
     4021 ccatagccgt ctatcatccc gacgggacca gcgaatactt cgagtataac gcgcacggac
     4081 aagccaccgt ggaaaagcgg cgcgacggca ccacctggac caacgcctat gaccttcgcg
     4141 gcaggcgaac cagcacaacg gggccgggcg gagcctgcac gggctatgga tatgacgaac
     4201 acgaccgtgt ttcatccgaa accaatgccc tggggctggt caccacaacg gaatacaact
     4261 gggcaggcaa accaacacga aaggtgtatc ccgattcaac agaggaagtc tggcagtacg
     4321 accgctacgg cagcgtcacg caggccgtca gccggtccgg cggaatcacg gtctatgagt
     4381 acgacgagcg cggcaatcaa attcgcgtga ccgaccccgt cggcgccgtg accgaaacca
     4441 cctacgatct cgatggaagg aaggttctgg aaaccagtcc aagcggcctg gtcgtcagca
     4501 acacctatga tgtccttggc agacggattc ggcgcaccta ttccagcgac aacaccttcg
     4561 aagcctggac ctatgtgtat gacggtgtca gcacccacac caaccggctc ggatatggca
     4621 cgacgaacgc ctataatgct gatggccaac tcgccagcgt caccgatacc cggggcaaca
     4681 caacccacta cacctatgat cagctcgggc gcagagtcgc catccaaaat gccttgggcc
     4741 agaccacaac ccagaccctt gacccgtccg gacgatttac ctccaccacc gatgatgaag
     4801 gtctggtagt gaccaatacc tacaacgcca acggtcaatg gattcgctcc gtccagcgcg
     4861 gcggcaccac caacgattat gcctatgata ccgccggtcg ccggacccat gtcgtgctga
     4921 acggcgcgac ggtttcgagt acggcgtatg atgccatcgg acgcaccgtt tggacccgca
     4981 acgccgacgg cgtggtggtg agtaacgcct acgactccgc cgggcggctg tatcgcgttt
     5041 atatgcccga cggcaccttc accgagaatg tctattccaa tggatggct
//
LOCUS       DAOPNP010000138         5022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_18185_length_5022_cov_9.415945, whole genome shotgun sequence.
ACCESSION   DAOPNP010000138 DAOPNP010000000
VERSION     DAOPNP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18185_length_5022_cov_9.415945"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PKI64_09950"
     CDS             complement(<1..1211)
                     /locus_tag="PKI64_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOE01360.1"
                     /translation="MLLDTFPTREEFRARCAEATVVPVGVRILADTETPVSVLARFAP
                     NHPRLFLLESAEGGERWGRYSFMGVTARATITVFRDDVVVRQGIAEQRTPHHNEPLTV
                     LRNLLKPYKLAHQPGLPRFCGGLVGYCAYEMIHFFEPRVPNTLPPERPLAEFVIPDTL
                     LIFDNINHTLTALALAFTEDGEPDALFDAASARVQDLLATLAQPIPAATTGKAPVRMP
                     QPVHPPEDFKAMVARVKEHIYEGDVIQTVVSQSFVGPAPADLVSLYRAQRHVNPSPYL
                     FFLKAQDSTLIGSSPETMIRLDQRTATLRPIAGTRPRGATEQEDRKLADSLLQDEKER
                     AEHLMLVDLGRNELGRVALPGTVQVTDLMIIERYSHVMHLVSNITADLEPGHDAFDLL
                     ASSFPAGTLSGA"
     gene            complement(1325..1984)
                     /locus_tag="PKI64_09955"
     CDS             complement(1325..1984)
                     /locus_tag="PKI64_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOE01361.1"
                     /translation="MSEKDFYAQKDLIKRLVRYKNILLRLKSMGLSRVFSDNLGDAAG
                     VSSSLVRKDFSLASLAVGNKRGGYSIDLLLRRLDDLLGAHDVQRIIIVGCGKIGTALL
                     NYKGFSNENIQVVAGFDNDPARINPANTPIPILPISQLEPFCQEQSIQVAVLAVPEKA
                     AAGILDKLSAAGIRGVLNFSPVQLKAGSALFIQNINIAFEIENLFYHVRFNQRQANAG
                     S"
     gene            2140..2628
                     /locus_tag="PKI64_09960"
     CDS             2140..2628
                     /locus_tag="PKI64_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE01362.1"
                     /translation="MTQTPDIQEKLTPALQEFIQQRKSQPGSLIMVLHRVQEEYGYVP
                     RSVAFQVADLLGIPLAQVYGVLTFYHFFKLKKPGKYRISVCMGTACYLKGGQDLLQEL
                     ENILGVGLNTVTPDGLFSVEAVRCLGCCGLTPVMSVNGAIHGNIGKEDVAGILAQYRA
                     RG"
     gene            2682..3074
                     /locus_tag="PKI64_09965"
     CDS             2682..3074
                     /locus_tag="PKI64_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOE01363.1"
                     /translation="MAKLKVADLAKLRKAQQAARQAQPAKAGNVELIVGTGTCGIAAG
                     ANETIAVLRAELQKAGLTNVTIRQTGCMGLCHSEPTVEVLMGGMPPVVYGRVNEDTAR
                     QIVHQHIGRGLLVNDHIYDKPMADFLPA"
     gene            3094..4881
                     /locus_tag="PKI64_09970"
     CDS             3094..4881
                     /locus_tag="PKI64_09970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE01364.1"
                     /translation="MAIKTYLLVCGGAECKNAHGTELHQALQKEIANEGVQAETQVVR
                     TGCFGLCAQGPIVKVLPDGTLYANVKPGDAREIVVEHIAKGQPVQRLLYNPAKSQETI
                     RLAEPTGYHKQFRIVLRNCGVIDPEKIEDYIARDGYAALAKVLTEMTPDRVVEELRKS
                     GLRGRGGAGFPTWLKWKFTKDAASDEKYVVCNADEGDPGAYMDRSTVEGDPHSILEAM
                     TIAGWTVGAQHGVVYIRAEYPLAIAHLETALKQSREYGLLGKNILGTGFDFDIEIRLG
                     AGAFVCGEETALLASAMGERGMPIPRPPFPAVSGLWQKPTVINNVETYANIPVIMLRG
                     GDWFAKVGTPKSPGTKVFALTGKINNSGLIEVPMGTTLREIIYDIGGGIRGGKKFKAV
                     QTGGPSGGVITAADLNTPIDYEHLQALGSIMGSGGMIVMDEDDCMVDVARFYQEFCVD
                     ESCGKCAPCRIGTRTLLNLLEQITVGKGTMEDLAKIKEVGTAMQKAALCGLGQTAPNP
                     VLSTMTYFADEYEAHIRDHRCPAGKCKALVTYTIDPEKCIGCTLCARRCPVNTIRGER
                     QKPHVIIQDNCIKCGECLHACKFGAVSRK"
     gene            4901..>5022
                     /locus_tag="PKI64_09975"
     CDS             4901..>5022
                     /locus_tag="PKI64_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE01365.1"
                     /translation="MVKLTINGLPVEVPEGVNILNAARQVQVKIPSLCYHPDLPP"
BASE COUNT         1078 a         1276 c         1715 g          953 t
ORIGIN      
        1 gcgccggaga gggtgccggc ggggaaggag gaggcgagca gatcgaaggc gtcgtggccg
       61 ggttcaagat cggccgtgat gttggaaacg aggtgcatga cgtgggagta gcgctcgatg
      121 atcatgagat cggtgacttg cacggtgccg gggagcgcca cgcggccgag ttcgttgcgg
      181 ccaaggtcca ccagcatgag gtgttcagcg cgttcttttt catcctgcaa gaggctgtcg
      241 gccagtttgc ggtcttcctg ctcggtggcg ccacgcgggc gggtgccggc gatgggccgc
      301 agcgtggccg tgcgctggtc gaggcgaatc atggtttcgg gcgaggagcc gatgagcgtg
      361 ctgtcctgcg ccttgaggaa aaagagatat ggcgaggggt tcacatggcg ctgggcgcgg
      421 tagaggctga cgaggtcggc gggcgcaggg ccgacgaacg attgcgacac cacggtttgg
      481 atgacatcgc cctcgtaaat atgttctttg acgcgcgcga ccatggcctt gaaatcttcc
      541 ggcgggtgga ccggctgggg catacgcacc ggggctttgc cggtggtcgc ggcgggaatg
      601 ggctgggcca gcgtggcgag caagtcctgc acgcgggcgg atgccgcatc gaaaagggca
      661 tccggctcgc cgtcttccgt gaaagccagc gcaagcgcgg tgagcgtgtg gttgatattg
      721 tcgaaaatga gcagcgtgtc gggaatgacg aactcggcca gcgggcgctc gggcggcaac
      781 gtgttgggca cacgcggctc gaagaagtga atcatctcgt aggcgcagta gccgactaat
      841 ccgccacaga agcgtggcag gccgggctgg tgcgccagct tgtaaggctt gagcagattg
      901 cgcagcacgg tgagtggctc attgtgatgc ggggtgcgct gctcggcgat gccttggcgg
      961 accacgacat catcgcgaaa aaccgtgata gtggcacgag cggtgacgcc catgaaggaa
     1021 tagcggcccc aacgctcgcc accttccgcg ctttcgagga ggaacaggcg ggggtgattc
     1081 ggcgcaaaac gcgccagcac ggaaactgga gtttcggtgt cggccaagat gcgcacgccg
     1141 acggggacga cggtggcctc cgcgcagcgg gcgcgaaact cctcgcgggt tggaaatgtg
     1201 tcgagcagca tggggaatga gtcctgtttc tggatgagtt cgctatgttt ctacatatag
     1261 aaacatggtc gggagggatt gtcaagcggt ggatgaaaat attttttgcg agaggagcgc
     1321 gaggctagga gccggcgttg gcttggcgtt gattaaagcg gacgtggtag aagagatttt
     1381 cgatttcaaa cgcaatgttg atattttgaa tgaacaacgc ggagccggcc ttgagttgca
     1441 cgggcgagaa gttgagcacg ccgcggatgc cggcggcgga gagtttgtcg agaatgcccg
     1501 cggctgcttt ttctggtacc gccagcacgg caacctggat actctgctct tggcagaatg
     1561 gctccagttg gctgataggg aggatgggga tgggcgtgtt ggccggattg atacgtgcgg
     1621 ggtcattgtc gaaccctgcg accacctgaa tgttttcgtt gctgaatcct ttataattaa
     1681 ggagagccgt gccgattttg ccgcaaccaa cgatgatgat ccgctggaca tcatgagcgc
     1741 cgagcaggtc atccaaacgg cgcagcagca gatcaatgga gtacccgccg cgcttgttgc
     1801 ctaccgccaa actggccagc gaaaaatcct tgcggaccaa ggatgaagaa acgccggcgg
     1861 catcgccgag gttgtcggaa aaaacacggg acagccccat ggactttagc cgcagcaaaa
     1921 tattcttgta gcgaactaga cgcttgatca aatctttttg ggcgtaaaaa tccttttcac
     1981 tcacaataaa cctcctgcac tctttttata tgtgacgaat aaaacatata ttgtgcaaat
     2041 atgcgcatgt caagtgggtt tggtgtcaga aatatcttgc cagcagggga gcaatttttt
     2101 actctccttc ccaattttgt ggaggcggca atggtaaacg tgacccaaac cccggatatc
     2161 caggaaaaac tgacaccggc gttgcaggag ttcatccagc agcgcaagag ccagcccggc
     2221 agcctgatca tggtgctgca ccgggtgcag gaggagtatg gctatgtgcc gcggtcggtg
     2281 gcgtttcagg tggccgattt gctggggatt ccgctggcgc aagtgtatgg cgtattgacg
     2341 ttttatcatt tctttaagct caagaagccc ggcaaatatc gaatcagcgt gtgcatgggc
     2401 acggcgtgct atctcaaggg ggggcaggat ttgttgcagg agttggaaaa tattctgggg
     2461 gtcgggctca acaccgtgac gcctgacgga ttgttttcgg tcgaggcggt gcgctgcctc
     2521 gggtgttgcg gtttgacgcc cgtgatgagt gtcaatggtg cgattcacgg caacatcggc
     2581 aaggaagatg tggccggaat tttggcacag tatcgggccc gcggatagtg caggagcagc
     2641 ggagcggatc tgagcaggat agcggcaagg cgaggtacaa aatggcaaaa ctgaaagtgg
     2701 cggatttggc taaactccgg aaagcgcagc aagcggcgcg tcaggcgcag cctgcgaagg
     2761 cggggaatgt ggaactgatt gtcggcacgg gcacgtgtgg gatagcggcc ggcgcgaatg
     2821 aaaccatcgc cgtgttgcgg gcggagttgc aaaaggccgg gttgaccaac gtgacgatcc
     2881 ggcaaaccgg ctgcatgggg ctgtgccaca gcgagcccac ggtggaagtg ctcatgggcg
     2941 ggatgccgcc ggtggtgtat gggcgcgtga acgaagacac cgcacggcag atcgtgcacc
     3001 aacacatcgg gcgggggctg ctggtgaatg accacatcta cgacaagccc atggcggatt
     3061 ttttgccggc ctaacggata gagaggaaac acaatggcca tcaaaaccta cttgctggtg
     3121 tgcggcggcg ccgagtgcaa gaatgcgcac ggcacggagc tgcatcaggc gctacaaaaa
     3181 gaaatcgcca atgagggtgt gcaggcggag acgcaggtgg tgcgcaccgg ctgcttcggt
     3241 ctttgcgcgc aggggccgat cgtcaaggtg ctgccggatg gcacgctgta tgccaacgtc
     3301 aagccgggag atgcccggga aatcgtggtg gagcacatcg ccaagggcca gcctgtccag
     3361 cggctgctct acaatccggc caagagccag gagaccatcc gtttggcgga accaaccggc
     3421 taccacaagc aattccggat tgtgctgcgt aattgcggcg tgattgaccc ggaaaaaatc
     3481 gaggactaca tcgcgcgcga cggctacgcg gcgctggcga aggtgctcac cgagatgacg
     3541 ccggaccgcg tggtggaaga gctgcgcaag tcgggcctac gcgggcgggg gggagcgggg
     3601 ttccccacgt ggctgaagtg gaagttcacc aaggatgccg ccagcgacga aaaatatgtg
     3661 gtgtgcaatg cggatgaggg cgatcccggc gcctacatgg accgctcgac ggtggaaggc
     3721 gatccgcact ccattttgga ggccatgacc attgccgggt ggacagtggg tgcgcagcat
     3781 ggcgtggtgt acatccgcgc ggaatatccg ctggccattg cgcacttgga aaccgcgttg
     3841 aagcaatccc gcgaatacgg tctgctggga aagaacatcc tcggcacagg atttgatttc
     3901 gacattgaaa tacggctggg cgccggcgcg ttcgtttgcg gcgaggaaac cgcgctgttg
     3961 gcctcggcca tgggcgagcg gggaatgccc attccgcgcc cgccgttccc ggcggtgtcg
     4021 ggactctggc aaaaacccac ggtaatcaac aacgtggaaa cctacgccaa catcccagtt
     4081 atcatgctcc gcggcggcga ctggttcgcc aaggtgggca cgcccaagag tccgggcacc
     4141 aaggtgttcg cgctgacggg caagatcaac aatagcgggt tgattgaagt gccgatgggc
     4201 actacgttgc gcgagattat ttatgacatt ggcgggggta tccgcggcgg caaaaagttc
     4261 aaagctgtgc agacaggtgg gccgtcgggc ggggtaatca cggcggcgga tttgaacacg
     4321 ccgattgatt acgagcatct gcaggcgctg ggctccatca tgggctccgg cggcatgatc
     4381 gtcatggatg aagacgattg catggtggac gtggcccggt tttatcaaga gttctgtgtg
     4441 gacgaaagct gcggcaagtg cgcgccatgt cgcattggca cacgcaccct gctgaatctg
     4501 ctggagcaaa ttacggtggg caaggggacc atggaggact tggccaagat caaggaagtc
     4561 ggcacggcca tgcaaaaggc ggcgttgtgc gggctggggc agaccgcgcc caacccggtg
     4621 ctgtccacca tgacgtattt tgccgatgag tatgaggcgc atatccgcga ccatcgctgc
     4681 ccggcaggca aatgcaaggc gctggtgacc tatacgattg acccggagaa gtgcatcggg
     4741 tgcacgctgt gcgcacggcg ctgcccggtg aacaccatcc gcggtgagcg gcaaaaaccg
     4801 cacgtcatca ttcaggacaa ttgcatcaag tgcggggagt gtctgcacgc ctgcaagttt
     4861 ggcgctgttt cccgcaagta agaggaaccc gacgatgaaa atggtgaaat tgaccatcaa
     4921 tggcctcccg gtggaagttc ccgagggagt caacattttg aatgccgccc gccaggtgca
     4981 ggtgaaaatc ccgagcctgt gctaccaccc cgacttgccg cc
//
LOCUS       DAOPNP010000139         4916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_18714_length_4916_cov_8.322773, whole genome shotgun sequence.
ACCESSION   DAOPNP010000139 DAOPNP010000000
VERSION     DAOPNP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18714_length_4916_cov_8.322773"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKI64_09980"
     CDS             complement(<1..886)
                     /locus_tag="PKI64_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01366.1"
                     /translation="MTNPNDLARRLAELFRLPGYRPLPERDLAKRLLLPSGQRGALRR
                     ALRDMAEQGAAATYGGGRWGAPTGGTQTITGTYQARLNGSGWLLPDTPDEKPCWLEPA
                     SILGAVHGDRIAARFIPRTPDMWIPGARPETKAARLTQILERPRAQAVGTVQWSARGV
                     HLLPRDPLLPTPIMLTDPPAKLKPLRGHLILASLSPAPAPPTRPVTARFQEDLGQPDD
                     PHNDIPALLKDHGVEENFPLEVQRAARQVATSVRRTPGPRRVDLRDRLILTIDPASAH
                     DQDDAISIARLPDGRWQLG"
     gene            1063..1827
                     /locus_tag="PKI64_09985"
     CDS             1063..1827
                     /locus_tag="PKI64_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE01367.1"
                     /translation="MATKTKGTTGITGRERQAKTKCGRAGAFCLLLVEGDQNLRRRLK
                     RMLLKTAPNISVIEANSVTTAIRRFDDNRPGTILLALALPDGDGLEVLRHAMAMDPHC
                     MVIVLTDHATDAVRLKCFEEGADFVFAKSTAVDRAVAIAQRASQRELARAPRTPQPKR
                     IQQARLRLKNKSGLHVVPAAQLVRLAQRFRAQIEVACNEHTADAKSLMDVLALGAEYN
                     MEITVTAEGRDALEGLTAIRSLVARGFHEKRAPAKV"
     gene            complement(1829..>2644)
                     /locus_tag="PKI64_09990"
     CDS             complement(1829..>2644)
                     /locus_tag="PKI64_09990"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOE01368.1"
                     /translation="YRNLRTTGMPESVHLCDFPLPGGLTRDEALENQMTQVQEVVGLA
                     RQLRADFNLKVRQPLAALHVVCRDEAILDLVRPLADIVADELNVKEVRFGHDETALAE
                     LSAKVNYKTLGPKLGGLMKKAVAAVAEFTPAHVATLLAGQSVEVVLDGRTIALEPNDL
                     VIQRTPKAGLAVASSGTLVVALETELTPALVQEGHARELVNRIQNLRKESELDVADRI
                     ALSITGDADLLAAVEAHREMIAAETLAEKLTTAAGGGDLDINGHPCGLVLAKL"
     assembly_gap    2645..2744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2745..4867)
                     /gene="ileS"
                     /locus_tag="PKI64_09995"
     CDS             complement(<2745..4867)
                     /gene="ileS"
                     /locus_tag="PKI64_09995"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOE01369.1"
                     /translation="MFKPVSSKHSFPRMEEDILALWEKEQTFQQSLANRRTAKEYIFY
                     DGPPFATGLPHYGHLLAGTIKDIAPRYQTMRGHFVERRFGWDCHGLPIEALAQEKLGL
                     AGAADIAAKGVDVFNETCRSMVLTYVEEWRKTVTRMGRWVDFDNDYKTMDVTFMETIW
                     WVFKQLWEQGRVYKSHRIMPYSWKLNTPLSNFEAGSNYQDVQDPSITVRVRLRDLPAG
                     LDPQGAPVFALIWTTTPWTLYGNLGICAGPGIEYVAVRDVADHNVYLLAAARLAAYYK
                     KPDQYEILAKWTGEALRGLAYEPLFPTFAGTANAFQILNDGFVSTEDGTGLVHMAPAF
                     GEDDFRVCKAAGIPLRDYLDDECKFTAAVPEFHGKFCKDADKQIIRSLKDTGKLVHQS
                     TLVHSYPFCPRTDTPLIYRAIEAWYVRVEDLRDRLVANNSTVHWVPQAVGEKRFGNWL
                     KEAKDWNISRNRFWGSCIPVWIAEDGQDMVCVGSIGELEQLSGQKVTDLHKHHVDKIS
                     FTRNGKTYRRTPEVLDCWFESGAMPYAQQHYPFERAEELAKFFPADFIAEGLDQTRGW
                     FYTLMLLGTALFDQSPYKNVIVNGLVLAEDGRKMSKRLKNYPDPGHIMNTYGADALRL
                     YMINSPIVRAEDLCFSEQGVMQSLRHLLIPWWNAYSFLVTYANVDGWKPREKGSEVRV
                     QEQPANTTPGQSSAAASGGALMDRW"
BASE COUNT          898 a         1435 c         1588 g          895 t
ORIGIN      
        1 cgcccaactg ccagcggcca tcgggcaggc gcgcgatgga aatggcgtcg tcttggtcat
       61 gcgcggaggc ggggtcaatg gtgaggatca gacggtcacg caaatccacg cgacgcggcc
      121 caggagtgcg gcgaaccgac gtggcgacct gccgcgccgc gcgttgcact tccagcggga
      181 agttttcttc cacgccgtga tccttgagca gcgcaggaat gtcgttgtgc gggtcatctg
      241 gctggccgag gtcttcctga aagcgggcgg ttacaggacg ggtggggggg gcgggggcgg
      301 gggataggct ggccagaatc aagtgacccc gcagcggttt aagcttggcg ggcggatcgg
      361 taagcatgat gggcgtgggc agcaatgggt cgcgcggcag caaatgcacg ccacgcgcgc
      421 tccactgcac agtgcccaca gcctgcgcgc gcgggcgttc gagaatctgc gtgagccgcg
      481 cggctttggt ttcggggcgc gcaccgggaa tccacatgtc gggcgtgcgc ggaatgaatc
      541 gcgcagcgat gcggtcgcca tgcaccgcgc ccagaatgga tgccggttcc agccagcagg
      601 gcttttcatc aggagtgtcg ggcagcagcc agccggagcc gttgagccgc gcctgatacg
      661 tgccggtgat ggtctgcgtg ccaccggtag gcgcgcccca gcgcccgccg ccataagtgg
      721 cggccgcgcc ctgttcggcc atgtcgcgca gggcgcggcg caacgcgccc cgctggccgg
      781 atggcagcag caggcgcttg gccaaatcac gttcggggag ggggcggtag ccgggcaggc
      841 gaaaaagctc tgccaaccgg cgggccaggt cattcggatt agtcattgcg ccaatcatgc
      901 gtagggcgcg agcggctggc gagtcttatt tgcggcggcg agtcacaggc cgccggcgga
      961 gcttgcatcc gaccaccaaa tggggcatac tcattatagg tgctcggcgc aatggggcgc
     1021 gagcggtttg gggccgggtg gcgatggcga aaggagttgg tcatggcgac gaaaacaaaa
     1081 gggacaacgg gaataacagg gcgcgagcgt caggccaaaa ccaagtgtgg gcgggccggg
     1141 gcgttttgcc tgttgctggt ggagggcgat cagaatttgc gccgtcggct gaagcgaatg
     1201 ctgctcaaaa ccgcgccaaa tatttccgtc atcgaggcca actcggtgac gaccgcaatc
     1261 cgccgctttg acgacaaccg gccgggcacg attctgctcg ccttggcgct gccagatggc
     1321 gatggcctcg aagtgctacg ccacgcgatg gccatggacc cccactgcat ggtgattgtg
     1381 ctgacggatc acgccacgga tgctgtgcgc ctgaaatgct ttgaagaagg cgcggacttc
     1441 gtgtttgcca aatcaacggc cgttgaccgc gcggtggcca ttgcgcagcg cgccagccag
     1501 cgggaattgg cccgcgcgcc gcgcacgccg cagcccaagc ggattcaaca ggcacgcctg
     1561 cggctgaaaa acaaatccgg cttgcacgtg gtgccggcgg cgcagttggt gcgtttggcc
     1621 cagcggttcc gcgcacaaat cgaagtggcc tgcaacgagc acaccgcgga cgccaagagc
     1681 ctgatggatg tgctggcgct gggggcggaa tacaacatgg aaatcactgt gaccgccgaa
     1741 gggcgtgatg cgctcgaagg cttgacggcc atccgcagcc tggtcgcgcg cggcttccac
     1801 gaaaaacgcg cgccggccaa ggtgtaaact acagcttggc caagaccagc ccgcaagggt
     1861 ggccgttgat gtccaagtcg ccgccgccgg cggctgtggt cagtttttcc gccagtgttt
     1921 cagcggcgat catctcacgg tgcgcttcca cggcggccag caaatcagca tcgccggtga
     1981 tgctaagcgc gatacggtca gccacatcca actcggactc cttgcggagg ttttgaatcc
     2041 ggttgaccag ctcgcgcgcg tgcccttcct gcacgagggc gggggttagc tcggtttcca
     2101 gcgccaccac cagcgtgccg ctggaggcta ccgccagccc agccttgggc gtgcgctgga
     2161 tcaccaaatc attgggttcc agcgcgatgg tccggccatc cagcacgacc tccaccgatt
     2221 ggccggccag caatgtcgcc acatgggccg gagtgaactc ggccactgcc gccaccgctt
     2281 ttttcatcag gccgcccaac ttggggccaa gggtcttgta gttcactttg gcggataact
     2341 ccgccagcgc ggtttcatca tgaccaaagc ggacttcctt gacgttaagc tcatcggcga
     2401 cgatgtccgc caaggggcgg accaagtcga gaatggcttc atcgcggcag accacgtgca
     2461 gagcagcgag tggttggcgt accttgaggt tgaaatcggc acgcaactgg cgggcaaggc
     2521 ccacgacttc ctgcacctgc gtcatttggt tttcgagcgc ttcatcgcgg gtgaggccgc
     2581 cggggagggg gaagtcgcag aggtggacgg attccggcat gccggtggta cgcaggttac
     2641 ggtannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatccag cggtccatca
     2761 gcgcgccgcc cgaggcggcg gcgcttgatt gtccgggagt agtgtttgcc ggctgctcct
     2821 gaaccctgac ctctgaaccc ttttccctgg gtttccatcc atccacattg gcgtaggtga
     2881 cgaggaagga ataggcattc caccacggaa tgagcaaatg gcgcagcgat tgcatgacgc
     2941 cctgctctga gaagcacagg tcttccgcgc gcacgatggg cgaattgatc atgtagagcc
     3001 gcagcgcgtc cgcaccgtag gtattcatga tgtgcccggg gtcgggatag tttttgaggc
     3061 gcttggacat cttgcgcccg tcctcggcca gcacgaggcc gttgacgatc acattcttgt
     3121 acggcgactg gtcgaacagc gcggtgccaa ggagcatcaa cgtgtaaaac cagccgcggg
     3181 tctggtcgag accttcggcg atgaagtccg ccgggaaaaa cttggcgagc tcttcggcgc
     3241 gctcaaacgg ataatgctgc tgggcgtaag gcatggcgcc tgactcaaac cagcagtcga
     3301 gcacttccgg cgtgcggcgg taggttttgc cgttgcgcgt gaagctgatc ttgtccacgt
     3361 ggtgcttgtg gaggtcggtg accttctgtc cggacagttg ttccagttcg ccgattgagc
     3421 cgacgcacac catgtcttgc ccgtcttcgg caatccatac cgggatgcac gagccccaga
     3481 aacggttgcg ggagatgttc cagtccttgg cctccttgag ccagttgcca aagcgctttt
     3541 cgccaacggc ttgcggcacc caatggacgg tgctgttgtt ggcgacgagg cggtcgcgca
     3601 agtcttccac gcgcacatac cacgcctcaa tggcgcggta gatcagcggg gtgtccgtgc
     3661 gcgggcagaa cggatacgag tgcaccagcg tggattgatg caccagcttg ccggtgtcct
     3721 tgagggagcg gatgatttgc ttatcggcgt ccttgcaaaa cttgccgtgg aactcaggaa
     3781 cagcggcggt gaacttgcat tcatcgtcga gatagtcgcg cagcggaatg ccggcagcct
     3841 tgcacacgcg gaagtcgtcc tcaccgaatg ccggcgccat gtggacgagc ccggtgccgt
     3901 cttcggtgct gacaaagcca tcgttgagaa tctggaacgc gttggccgta ccggcaaacg
     3961 tcgggaaaag cggctcatag gccaagccgc gcagcgcctc gcccgtccac ttggcgagaa
     4021 tttcgtattg gtccggtttt ttgtagtagg cggcgaggcg cgcagctgcc aacagataca
     4081 cgttgtggtc ggccacgtcg cgcaccgcca catactcgat gcccggcccg gcgcagatgc
     4141 ccaggttgcc gtagagcgtc cagggcgtgg tggtccaaat gagcgcaaaa accggcgctc
     4201 cttgcgggtc caacccggcg ggcagatcgc gcagccgcac acgcaccgtg atggagggat
     4261 cctgcacgtc ctgatagttg ctgccggcct cgaagttcga cagcggcgtg ttgagcttcc
     4321 atgagtaggg catgatgcgg tgcgacttat agacccgccc ctgttcccac agttgcttga
     4381 acacccacca aatggtttcc atgaacgtga cgtccatggt tttgtagtcg ttgtcaaagt
     4441 ccacccaacg gcccatgcgg gtgactgttt tgcgccactc ttccacatag gtcagcacca
     4501 tcgagcggca ggtctcattg aatacgtcca cccccttggc cgcgatgtcc gccgcaccgg
     4561 ctaaaccaag cttctcctgc gccagcgctt caatcggcag cccgtgacag tcccagccga
     4621 aacgacgctc cacaaagtgc ccgcgcatgg tttggtagcg cggggcgatg tccttgatgg
     4681 tgcccgccag caagtgaccg tagtggggca ggcccgtggc aaacggcggg ccatcataaa
     4741 aaatatattc ctttgccgtg cgccggttgg ccaacgattg ctggaaagtt tgctcctttt
     4801 cccagagggc gaggatgtct tcctccatcc ggggaaagga gtgcttgctg gaaacgggtt
     4861 tgaacatgag tctatccttt ggggcaaatc agccgctcat tcaacggcgc gcgccc
//
LOCUS       DAOPNP010000140         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_18942_length_4870_cov_8.963448, whole genome shotgun sequence.
ACCESSION   DAOPNP010000140 DAOPNP010000000
VERSION     DAOPNP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_4870_cov_8.963448"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKI64_10000"
     CDS             <1..262
                     /locus_tag="PKI64_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOE01370.1"
                     /translation="FDMGKRGKVAGCMCLKGKVTLKGRARVRRKDEILYEGKIQSLRR
                     FQNEATEVREGQECGIVLDRFTNFVEGDLIESYEIEKVAQEL"
     gene            293..691
                     /gene="rbfA"
                     /locus_tag="PKI64_10005"
     CDS             293..691
                     /gene="rbfA"
                     /locus_tag="PKI64_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOE01371.1"
                     /translation="MKADRITRVNELIRRELGVQLYRIINRPDFDAAAVTFTHALTSV
                     DLRNCRVLVSIRAPEADQQRMLGILHQHRAEFQKNISRNVILRYTPHLHFVLDHSVAE
                     GDNVLALLHQMEERGEVPADADPAEETPPG"
     gene            697..1419
                     /gene="truB"
                     /locus_tag="PKI64_10010"
     CDS             697..1419
                     /gene="truB"
                     /locus_tag="PKI64_10010"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOE01372.1"
                     /translation="MAASLNLGPLDGLLLVDKPAGPTSHDIVAQVRRHFRIAKVGHGG
                     TLDPMATGLLILLLGKGTKISERLMGHDKTYEGAMRLGIETDSQDADGQPVAEHDASG
                     ITREQIVAQMQARLGDQMQTPPMVSAIKINGVPLYKLARKGQTVERPPKLIHVYRFDL
                     LDYTPPDARFVVECTKGTYVRTLAHDIGQALHVGAHLVQLRRTHIGKFTVADALPLAD
                     LLALPSVADRVIPLAAALPLLG"
     gene            1425..2354
                     /gene="ribF"
                     /locus_tag="PKI64_10015"
     CDS             1425..2354
                     /gene="ribF"
                     /locus_tag="PKI64_10015"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOE01373.1"
                     /translation="MDTAAPTATAAAPLVLAIGQFDGVHRGHQAVFRATVEEARRHNG
                     VAGCLTFWPHVAQLLNPARAPLLLATPEQNRAWMTACGITQIHTLPFTRELAATGPGD
                     FLVMLKQQFPQLVGLVVGENFTCGKNRAGNIATLPALAAAQQLAIRFVPHVELAGAPI
                     SSTRIRAAVAVGDLPLAKAMLGRSFSLTGIVIHGRAVGRKLGFPTANIHPHLPIRPQP
                     GIYAARLLLPEGPRPGAAFVPDAHDPAQAHFGSVVEIHLPDYTGNLYGQTVEIAFTRR
                     LRGHQVFADADAAAAQIARDTTAALIADAEENT"
     gene            2351..4738
                     /locus_tag="PKI64_10020"
     CDS             2351..4738
                     /locus_tag="PKI64_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOE01374.1"
                     /translation="MSPAPRAPELLAPAGERAAAYAAFAFGADAIYTGLPRFSARAGA
                     VNLSPRELEEIIAYAHALPRPRRVYVTLNTLLYNAELPDLLRSLALCAEVGADAIIVQ
                     DAGVARLARKHFPTLRRHASTQMALHNLAGVRAAVRLGFQRVTLARELTVAEIGAIAR
                     ETDCEIETFLHGALCYSYSGLCQYSALLRGRSGNRGACAYPCRECFRPQAAAGRQPDT
                     AGGLIFSMKDLAGEAAAHDLARAGVASLKIEGRKKSPLYVAAVVNYYRKLLDRTFQPG
                     EQEQCARDIQTIFSRPWTSLYLSSRNQRDVIDADTTGHRGTLAGEVAAAGRDYLQFTT
                     RLPLEVHDGLQVELSGETRPFGFAVEEIILPNGRRVFTVPAHSRVQVPLPPGAPRIPP
                     GTTLFLASSQAVKRRYHFALPNPREWRRRYPVDIHVEFSADRLAATATLHADGGDLST
                     TLTREGPFAPAENPAAVTAAWQQAFAKLGDTPFQLHRLTQAGPVCFLPMAQLNALRRE
                     LAEQLTQAQRAAAEQSWQAAVQTLATTPPATPPRATGWLLKTDQPTAAVAALLAGGFP
                     PPAELIVELGRETPAQIESALGQLPTGITVRFALPMITRGWEQAALDDQIRHFWTAGH
                     RRWEAANPAALALLPATNELDLSADWPLYTLNTTAWAEWQAQGIRRGTVAPEDTPDNA
                     ALLARQYPWQLVWPVHRDPPLFISESCPHAAQLGHCPGPKACTITEQAYRSPAGETVL
                     IVNRHCRFYTLHEQPQWVPVPPSLPVVPRADFLYREWTPDAIRDACATLARTLPT"
BASE COUNT          838 a         1761 c         1436 g          835 t
ORIGIN      
        1 cttcgacatg ggcaagcgcg gcaaggtggc cggctgcatg tgcctgaagg gcaaggtcac
       61 gctcaagggc cgcgcgcgcg tgcgccgcaa ggacgaaatc ctctacgagg gcaaaatcca
      121 atccttgcgc cggttccaga acgaagccac cgaagtccgc gagggccagg agtgcggcat
      181 tgtgctggat cggttcacga actttgtgga aggcgacctg atcgagtcct acgagatcga
      241 aaaggttgcc caggaactct aggccgcccg gcgcggccgc cgccagtccc cgatgaaagc
      301 cgaccgcatc acccgcgtca acgaactgat ccgccgcgaa ctcggcgtgc agttataccg
      361 cattatcaac cgtccggatt tcgatgcggc agcggtcacc ttcacccatg cgctgacttc
      421 agtggatttg cgcaactgcc gtgtgctggt ctccatccgc gcaccagagg ccgaccagca
      481 acggatgctg ggcatcctgc accaacaccg tgccgagttc caaaagaaca tcagccgcaa
      541 cgttatcctg cgctataccc cgcatttgca ctttgtgctc gaccactccg tggccgaagg
      601 cgacaacgtc ctggcgctgc tccaccaaat ggaagaacgc ggcgaagtgc cggccgatgc
      661 cgacccggcg gaggaaaccc cgcccgggtg agcatcatgg ccgcctcctt gaacctcggt
      721 ccgctcgacg gtttgctgct ggtggacaag ccggctggtc ccacctcgca cgacatcgtg
      781 gcccaggtcc gccggcattt ccgcattgcc aaggtgggcc acggcggcac gttggatccg
      841 atggccaccg ggctactgat tctcctgctc ggcaagggca ccaaaatttc cgaacggctc
      901 atgggccacg acaagaccta tgaaggcgcc atgcgcctcg gcatcgaaac cgattcgcag
      961 gatgccgacg gccagcccgt ggctgaacac gacgccagtg gcatcacgcg cgaacaaatt
     1021 gtagcgcaga tgcaggcgcg gctgggcgac caaatgcaaa ccccgccgat ggtcagcgcc
     1081 atcaaaatca atggcgtgcc gctctacaaa ctggcccgca aggggcagac cgtcgagcgc
     1141 ccgcccaagt tgattcatgt ctatcgtttt gatctgctgg actacacacc gccggacgcg
     1201 cgctttgtgg tcgaatgcac caaaggaacg tatgtgcgca ccctcgcgca cgacattggg
     1261 caggcgctcc atgtcggcgc gcatctcgtg cagttgcgcc gcacgcacat cggcaagttt
     1321 acagtggccg acgcgctgcc cttggccgat ctgctggcgc tgccctccgt ggcggaccgg
     1381 gtcatcccgc tggccgcggc cctccccctg ctggggtgaa cctgatggac accgccgctc
     1441 caaccgcaac tgccgccgcg ccgctggtgc tggccattgg ccaatttgat ggcgtgcacc
     1501 gcggccacca agcggtcttc cgcgcgacag tggaagaggc gcgccgccac aacggcgtcg
     1561 ccgggtgcct gactttttgg ccgcacgtgg cccaactgct caacccggcg cgcgcccccc
     1621 tgctgctggc cacgcccgag caaaaccggg cgtggatgac cgcgtgcggt atcacgcaaa
     1681 tccacacgct gcctttcacc cgcgagctgg ccgccaccgg ccccggcgat tttctcgtca
     1741 tgctcaaaca acaattcccg caattggtcg gcctggtcgt cggcgaaaat ttcacatgcg
     1801 gcaaaaaccg cgcaggcaac atcgccacgc tgcccgccct ggcggccgct caacaactgg
     1861 cgattcgctt tgtgccgcac gttgagttgg ccggcgcgcc aatatccagc acgcgcattc
     1921 gcgcggcggt ggccgtgggc gatctgccgt tggccaaggc catgctgggg cgttcgttca
     1981 gtctgaccgg tatcgtcatt cacggtcgcg ccgtgggccg caagttgggg ttccccaccg
     2041 ccaacatcca tccgcacctg cccattcgcc cgcaacccgg catctatgct gcgcggctgc
     2101 tgctaccgga gggcccccgc cccggcgccg ccttcgtgcc ggatgcccac gaccccgccc
     2161 aagcccactt cggcagcgtg gtggaaatcc atttgcccga ctacacgggc aacctctacg
     2221 ggcaaacggt ggaaattgcg ttcacgcgcc ggctgcgggg ccatcaggtc ttcgccgatg
     2281 ccgacgcggc cgccgcgcaa atcgctcgcg acaccaccgc ggccctgatc gcggatgctg
     2341 aagaaaatac gtgagccccg ccccccgcgc ccctgaactg ctcgcgcccg ccggcgaacg
     2401 cgccgccgct tatgccgcct tcgcgtttgg cgcggatgcc atctataccg gtctgccacg
     2461 cttttccgcg cgcgctggtg cggtcaacct ctccccgcgc gagctggaag aaatcattgc
     2521 ctacgcccac gccctgccgc ggccgcgccg cgtgtatgtg acgctcaaca ccctgctcta
     2581 caacgccgaa ctgccggacc tgctccgctc ccttgcgctc tgcgccgaag tcggggccga
     2641 tgccatcatc gtgcaggatg ccggcgtggc gcggttggcg cgcaaacatt ttccgacgct
     2701 gcgccgacat gcctccacgc agatggcgct gcacaatctg gccggcgtgc gcgcggccgt
     2761 ccgcctcggc ttccagcggg tgactctcgc gcgcgaattg acggtcgcgg aaatcggcgc
     2821 catcgcgcgc gaaaccgact gcgagattga aaccttcctg cacggcgcgc tgtgctactc
     2881 ctactccggg ctgtgccagt attccgcgct actgcgcggg cgttcgggaa accgcggcgc
     2941 gtgcgcctac ccctgccgcg aatgcttccg gccgcaggcc gccgccggac gccagccgga
     3001 caccgccggc ggcctcattt tttccatgaa ggacttggcc ggcgaagccg ccgcgcacga
     3061 cctcgcccgc gccggcgtgg cctcgctcaa aatcgagggc cgcaaaaaat cgccgctcta
     3121 tgttgccgct gtggtgaact actaccgcaa actgctcgac cgcaccttcc agcccggcga
     3181 gcaggaacaa tgcgcgcgcg acatccaaac cattttctca cggccttgga cgagcctgta
     3241 tctttcctcg cgcaaccagc gcgacgtaat tgatgccgac accaccggcc accgcggcac
     3301 gctggcgggc gaagtcgccg ctgccgggcg cgattatctg caattcacca cccgcctgcc
     3361 gctggaagtt cacgatggcc tgcaagtgga actttccggt gaaacccgtc ccttcggttt
     3421 tgccgtcgaa gaaatcatcc tgcccaacgg ccgccgcgtg ttcaccgtac ccgcgcattc
     3481 gcgtgtgcag gttccgctgc cccccggtgc gccgcgcatc ccgccgggca ccacgctttt
     3541 tctggcctcg tcgcaggcgg tcaagcggcg ctaccacttt gccctgccca acccgcgcga
     3601 atggcggcgg cgctatcccg tggacatcca tgtggagttc agcgccgatc gcctcgccgc
     3661 caccgccacg ctacatgccg atggcggcga cctgtccacc actctcacgc gcgagggccc
     3721 ttttgccccg gcggaaaatc cggcggccgt cactgcggca tggcagcagg cgttcgccaa
     3781 attgggcgac acccccttcc agttgcaccg gctcacgcaa gccggcccgg tgtgttttct
     3841 gccgatggcg cagctcaatg ccttgcgccg cgagctggcc gaacagttga cgcaagcgca
     3901 gcgcgcagcc gccgaacagt cgtggcaagc ggcggtgcaa accctggcga caacgccgcc
     3961 ggccactccg ccgcgcgcga ccggctggct gctcaaaacc gatcagccca ccgcagccgt
     4021 agccgcgctg ctcgccggtg gttttccgcc gcccgccgaa ctgattgtgg aactcggccg
     4081 cgaaactccg gcgcaaattg aatccgcgct ggggcaacta ccgactggca tcacggtacg
     4141 tttcgcgttg cccatgatta cccgcggctg ggagcaagcc gcgcttgatg atcagatccg
     4201 ccatttttgg accgccggcc accgccgctg ggaagcggcc aatccggcgg cgctggccct
     4261 gttgccggca acgaacgagc tggacctcag cgccgattgg ccgctctaca ccctcaacac
     4321 taccgcttgg gcggagtggc aggcgcaggg catccgccgc ggcaccgtgg cgccggagga
     4381 cactcccgat aacgcggcgc ttctggcacg gcaatatccg tggcaactgg tgtggccggt
     4441 gcaccgcgac ccgccattat tcatttcgga atcgtgcccg cacgccgcgc agctcggtca
     4501 ctgccctggt cccaaagcct gcaccatcac ggagcaggcc taccgttcgc ccgccggcga
     4561 aaccgtgctg atcgtcaacc gccactgccg cttctacacg ctgcacgagc aaccgcaatg
     4621 ggtccccgtg ccgcccagcc tgccggtggt gccccgcgcc gattttctct accgcgagtg
     4681 gactcccgac gccatccggg acgcgtgcgc cacgctggcc cgcaccctgc ccacctgact
     4741 tttccacagt gtggaaacta tttttccaca gcgtggaact tttagatctg atttttccat
     4801 agcgtggaac tttttctccc cgtttttcca cagtgtggaa aaatatcctc aaagttgttc
     4861 cattgtgtgg
//
LOCUS       DAOPNP010000141         4745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_19620_length_4745_cov_5.619829, whole genome shotgun sequence.
ACCESSION   DAOPNP010000141 DAOPNP010000000
VERSION     DAOPNP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19620_length_4745_cov_5.619829"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..869
                     /locus_tag="PKI64_10025"
     CDS             105..869
                     /locus_tag="PKI64_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01375.1"
                     /translation="MNTSGKFFSYDGYRQFLTALKQAGTCVRFRDWSEDKRVILLRHD
                     VDLSVESALRLARIENELGVTATYLFRVSGNSYNVFASDNQHMIREIAACGFEVGLHF
                     DPSVYGELAEDALIPLARVEADALASVAQQPIHTVSLHAPSIHGQWPQIAGFINAYDP
                     RIFSDECYLSDSRMMFRGKDPFVFIQKAAERTIQILLHALHFGPTNRTYPKILADHLV
                     SVIRTLDEEWRLCSSTYCDQIEGSLLDYLTRREGKA"
     gene            866..1897
                     /locus_tag="PKI64_10030"
     CDS             866..1897
                     /locus_tag="PKI64_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOE01376.1"
                     /translation="MNIYILSSHGTGMDVLDVVSQTVPITGVIGLSDRPASDSISGYV
                     YLEPYCRTRGIPFYPVESYGLSREEDRIRLLQLEIDVLLVINWQRLIPDWLIQHCRYC
                     AIGAHGSAYGITGGRGRSPQNWALLMGKKSFHISIFRIDAGIDSGAVIDSREFPLSET
                     DDIATSYHKTFWLVAQMVAENIRNGRIQAGHLTEQKDAPAYLPKRTPEDGEIDWSRSA
                     HDVYNFVRALTRPYPGAFSRWGDSLVKIWHCRPFAVPGGKMKPPGTILAVFTSGDWLV
                     QAGDMPVLVYDYDVGESPIMTGECFSACSFKEQMKRIIARHVQDYPDLPVAEDIYRFA
                     GLEPLTGSL"
     gene            2201..2968
                     /locus_tag="PKI64_10035"
     CDS             2201..2968
                     /locus_tag="PKI64_10035"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE01377.1"
                     /translation="MAFSEDFTIMAEGTMTSASVSPRSICVAVMCFNEAASLPVVVAE
                     ITDELQRLGGDYEVVIINDGSTDGSGEIADSLAKESAHIRVIHHPRNLGLGAVYRNAF
                     LCGTKELTTVFPADGQFAPEIIGQFARLFDDMDLVLGYIPEFRKNRSFAARTFSWAER
                     CLYRLLFGRFPDFQGIMMFRRELVDTIELSSSGRGWMIQMELILRFIRKGYRVISEPT
                     GIRARMSGESKVMNLSAIASNLRQVFALRWNLWRHPG"
     gene            3346..4611
                     /locus_tag="PKI64_10040"
     CDS             3346..4611
                     /locus_tag="PKI64_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01378.1"
                     /translation="MKMALELTGRRFVAAAAGVVLALLPNLTETFHWSTQVLNEVLCA
                     LVPYALSGWLWVAYVRRGGMWRLVVSLLAYGVGIFSYEVGVLLPGAYLLLLPWKRQPI
                     KSVWRMAPFGAMFLFYMGWRATGAYGLFQTWQYPPHMQASVSAYGLALKSWHLLHWWV
                     GEYMFGAMLNGFEYFSAIPLWPKRFLFVANAVVSALVIWGLCRMAKSENSANPKPFGN
                     AQVVAFALAWVGAAWVVSLASYTCGRLNVLPAIGVSMLAALVLARLPARQWSLLLVVP
                     IFLALLANQGTAEAHRQAGQINRAIYEHLQASQGEWADKKLIVMDTTGLRERQTAGLL
                     RHSTFSDVTWAQCGNAPFARGFTFSAMVKMIAGTDQSQPQVLHDMEYGTRRADDQLLW
                     HDRYNDTRPHTNALADVFWVDCLAVAQGR"
BASE COUNT          981 a         1173 c         1370 g         1221 t
ORIGIN      
        1 tcaaaaaagc ccgcgacctc atcggcttcg aagccgaagt ggatttggaa gaggggatca
       61 agcgcacggc ctccttttat cagaattaat tatacggcct tggtgtgaat acgtccggaa
      121 aattcttttc atacgacggt tatcggcagt ttcttacggc tctgaaacag gccgggactt
      181 gtgtccgttt ccgagattgg agcgaagaca agcgggtcat cttgctgcgc cacgatgtag
      241 atttaagcgt cgaatccgca ctaaggctcg cccgaattga aaatgagctg ggtgtcacag
      301 ccacttattt gtttcgggtt agtgggaatt catacaacgt atttgcctct gataaccagc
      361 atatgattcg tgaaattgcg gcttgcgggt ttgaagtcgg cttgcatttt gatccctccg
      421 tgtatggtga actcgctgaa gatgctctga ttccgctggc gagagtagag gccgacgcat
      481 tggcttctgt tgcccaacag ccgatccaca cagtaagtct tcacgcccca tccattcatg
      541 ggcaatggcc acaaatcgcc gggttcatca atgcctacga cccgcgcata ttttcagacg
      601 aatgttatct gtcagattcg cgcatgatgt ttcgcgggaa agacccattt gtgtttatcc
      661 agaaagccgc ggagcgcaca atccagatcc tattgcatgc cttgcacttt gggccaacca
      721 atcgaactta tcccaaaatt ttggctgacc atctggtgtc ggttattcgg accttggatg
      781 aagaatggcg actttgcagt agtacctatt gcgatcagat tgagggttcg ctattggatt
      841 acttaacccg cagggaaggc aaggcatgaa tatctacatt ctttcgtcgc acggcaccgg
      901 catggatgtc ttggatgtgg tgtcgcaaac tgtgcctatt acaggcgtca ttgggcttag
      961 tgaccgtccc gcttcagact caatctcggg ttatgtctat cttgaaccat actgccgtac
     1021 gcgcggaatc ccgttttatc ctgtagaaag ctatggcctg tcgcgggagg aagatcgaat
     1081 ccggttacta cagttggaaa tagacgtact gctggtgatt aattggcagc ggttaatccc
     1141 tgattggttg attcaacatt gtcgctactg tgcaatcggg gcccatggca gtgcttatgg
     1201 catcacgggc ggacggggac gctctcctca gaactgggct ctcctgatgg gcaagaagtc
     1261 ctttcatatt tcaatcttcc gcattgatgc cgggattgat tccggagccg taatcgactc
     1321 tcgcgagttt ccactgtctg aaacagacga tattgccacc tcgtatcata aaactttctg
     1381 gcttgtcgct cagatggtag cggagaacat acgcaatgga cggattcagg ccggccatct
     1441 tacggaacaa aaggatgccc cggcttattt gcccaaacgc actccggaag atggggagat
     1501 tgactggagt cgctccgcac acgacgtgta caattttgtg cgtgctttga ctcgccccta
     1561 tcctggtgca tttagccgat ggggggatag tttggtaaaa atttggcatt gccgaccgtt
     1621 tgcggtgccc ggcggaaaaa tgaagccacc aggaacaatt ttggctgttt tcacatcggg
     1681 cgactggctg gtccaagcag gtgatatgcc tgttctagta tacgattacg atgttgggga
     1741 aagccctatc atgaccggag agtgcttttc tgcatgcagt ttcaaggagc aaatgaaacg
     1801 cattattgcg cgacatgtgc aagactatcc cgacctcccc gttgcagaag acatctaccg
     1861 ttttgccggt ctagagccac ttacggggtc gttatgagct ttcagattgt catcaaactt
     1921 ggcatggagt gggcgactga ttgtactatc gagaatagcg cgagtgttct tcggttgaaa
     1981 caagcccaac ccaccgggtt cgtaactcat cctacgccga ccttaatcgg gctgcgtgcg
     2041 gccccggacc ttgggccaag gcttttgtac cgtcttgcac ttctgctgaa atgatgaaaa
     2101 gactgatcca tctctttgtg gaattatcca cgaactacgc atccccggcg tcaaaaaagc
     2161 cagcgaccac atcgaatccg aagccgaagt ggattggaca atggcatttt cagaagattt
     2221 cacaatcatg gctgaaggca ccatgacgtc agccagtgta agtccacgca gcatctgcgt
     2281 ggcggttatg tgtttcaatg aagccgccag cttgccggtg gtggttgcgg agatcacgga
     2341 tgaattgcag cgcttggggg gcgactacga ggtggtcatc atcaatgatg gcagcaccga
     2401 cggcagtggc gaaattgcgg atagcttggc caaagagtca gcccatattc gagtgatcca
     2461 ccacccccgc aatttgggac tgggagccgt gtatcgcaat gcgtttttat gtggaacaaa
     2521 agaactcaca accgttttcc cggcggacgg tcaattcgcc ccggaaatta ttggccaatt
     2581 tgctcggctt ttcgacgata tggacctcgt gctggggtat atccctgaat ttcgaaaaaa
     2641 ccgatccttc gctgcccgta ccttttcttg ggcagaacgc tgcctgtacc gacttttatt
     2701 tggccggttc cctgattttc agggcatcat gatgtttcgc cgcgagttgg ttgacacgat
     2761 tgagctgtct tcttccggcc gtggctggat gattcagatg gaattgatcc tgcgcttcat
     2821 caggaaaggc taccgggtga tcagcgaacc gactgggata cgtgctcgga tgagtggtga
     2881 atccaaggtg atgaatttat cggctatcgc ctctaatttg agacaagtat ttgctcttcg
     2941 ctggaacctc tggcgtcacc cgggataata gattgcgcgc ccagatcatg ccgccctatt
     3001 ctggtgccag agcttgtgag gcctccgagt atgaatagtg acgtccatcc ccaaatcaac
     3061 gcgccacata gtgggctgtg gtttattatt ctgctcacct tgcttttcca aatctggtcg
     3121 cggtttgtgc catcgcctca agccgtttta atcgcggatg actgggctaa tttggcgaaa
     3181 agtcagccga tggcggactg gcaccaatgc gtggcggcgg gatttcaaga tcctcatcgg
     3241 ccgttgagca cgactgccgt gtatctcgga tttcggtttt tggacgcgcc cgcatactgg
     3301 accttaatcg ggctagtggg caattcgttg ttgttgctct ttgcgatgaa gatggcgctg
     3361 gagttgacgg ggcggcggtt tgtggcggcg gcggcggggg tggtgctggc gttgttgccg
     3421 aatttgacgg agacttttca ctggtccacg caggtgctca atgaagtgct gtgtgcgttg
     3481 gtcccgtacg cgttaagtgg gtggctgtgg gtggcctatg tgcgccgcgg cgggatgtgg
     3541 cggctggtgg tttctttatt agcttatggc gtggggattt tctcctacga ggtcggggtg
     3601 cttttgcccg gcgcttatct cctgctgttg ccttggaagc gccagcccat taaaagcgtg
     3661 tggcgcatgg cgcctttcgg cgccatgttc ctattctaca tggggtggcg ggcaactggc
     3721 gcttatgggc tgtttcaaac gtggcagtat ccgccgcaca tgcaggccag cgtttccgcc
     3781 tatgggcttg cgttaaaatc atggcatctg ctgcattggt gggtgggcga atatatgttc
     3841 ggcgccatgt tgaatgggtt tgaatacttt tccgctattc cactatggcc caagcgattc
     3901 ttgtttgtag cgaatgccgt ggtgagtgcg ctggtgattt ggggcctgtg ccggatggca
     3961 aaatccgaga actccgcaaa cccgaagccg tttggcaatg cgcaggttgt cgcttttgct
     4021 ttggcgtggg tgggtgcggc gtgggtggta tcgttggcat cctacacatg cggcaggctg
     4081 aatgtcttgc cggccattgg cgtgagtatg ctggcggcat tggtgctggc acgattgccc
     4141 gcgcgccagt ggagcttgtt gctggttgtg ccgatttttc tggctctgct ggccaaccaa
     4201 gggaccgcgg aggcccaccg ccaagccggc cagatcaatc gcgcgattta tgagcatttg
     4261 caagcatcac aaggcgagtg ggcggataaa aagctgattg tgatggacac caccggactg
     4321 cgcgagcggc agacggccgg gctgctgcgg cacagcactt tttccgatgt cacttgggcg
     4381 cagtgcggca acgcgccatt tgcgcggggt tttacttttt cggccatggt caaaatgatt
     4441 gcgggcacgg atcaatccca accgcaagtt ttacacgaca tggaatacgg aacccgccgt
     4501 gcggatgacc aactgttgtg gcatgaccgc tataatgaca cgcgccccca caccaacgcg
     4561 ttggccgacg ttttttgggt ggattgtcta gccgttgccc aaggccggta ggcacaccct
     4621 tggggtgccg gggaaacggt ccatggcgtg gccggatggc ttacgcgggc ggggttgagg
     4681 tggattcagt gcggttttgt ttgagccagg cttcgataaa gagacccaag tcaccgtcga
     4741 gcacc
//
LOCUS       DAOPNP010000142         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_20203_length_4629_cov_4.997814, whole genome shotgun sequence.
ACCESSION   DAOPNP010000142 DAOPNP010000000
VERSION     DAOPNP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20203_length_4629_cov_4.997814"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..>4629
                     /locus_tag="PKI64_10045"
     CDS             460..>4629
                     /locus_tag="PKI64_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE01379.1"
                     /translation="MNVGLYGGTAEASKSRTNEWLFAMSFNDGGTLIGTSRVEWVGGN
                     LGTNATVNLQYTTNNGVSWESIATGVTATNESYTWAPDFDYPAVLWRVVHTNSGVAST
                     NAKVFSVRRSTNVVFNFYVNDNSTDNDVYCSALGSSTNDGAASSRPLRSLQSVVNRYQ
                     LRGGDTVYVDTGDYATNVTITIGGFDSGVAGKPVKIIGSPKGSVFNRGNIGANVLDLA
                     GASYLEIENLKLTGGAAGLNGGVSNIVLRGLQFIGNTHGVVINGAAHTFENCVAADNT
                     QRAFNGSGSGANVWRNGVMWGSPTLVFAPTNATLSVSNSILGNGTTLFGSQIIPGDFN
                     LVWNVGVGLSYQAFTDFQNAGYWTNSLYADPVFANAAGGDFHLKSVMGRYNPATGLFE
                     TNDAVHSPGIDLGDPSAIYTNEPVPNGSRLNVGLHGNTAQASKSRTNAWLQIRSYTDG
                     GTLDAQAGSWLRWNGANFDPSATVTLWLSRDNGVSWETLATNQMATNGAYFYQNTSSN
                     DYSALYAMWKVTLDNATPSVSSQSPTNFNYRNGAYAFYVNDASTNGDVYCTAPGSDSN
                     LGVSPGAPMQSLHTLINTYKIGPGDRVYIDTGVYTATNTVLITAQDSGGATNPVFIIG
                     STNRLAGGSLFRSPAIPQRPLAFNISGAASNIILRDIVVSNASRGVMVSNALNIRLEG
                     VEVRGAGQTAFELANNARSVTVTRCVAHGGGIGISLQQATNIVVNHSLFWQNTNNAVY
                     LGTQVGLLLENSILASTNFGAALYSVPSNSPTVTMDYNGIHAGPNTRVGINRTTGARA
                     DSLVAWQYLANDQDVHSIANNPLMADPDAFDYHLLTEQALGRIMPNGQRTTDPVSSPL
                     LDAGNPASDFSAEPAPNGGRVNIGVFGGSAQASSARVSPWLKAISFGDAGYVTNGTFT
                     LRWIAGGELSNETVKVEVSVDGGRSWTQTVASGVAATNGEATWNVADLPDTPAGVWRV
                     TGLQRTNVWSGSSNFFAIRNQPLNLYVNGIYTNEAVYTTAAGRPDNWMATSNAPLDSL
                     RVVFERFDLEPGDSIWVDPAVYLEPAAISISLKNSGTTSQPIRVTGPLNKPYTGAILA
                     RTSRGSGSYVIQLVNARGIEFSGLVVSNAFIGIYEQNSQGILWNKGRVSHCVTNGIFA
                     GSGADLTVSHSIIENSLTAGLQTLTGSTIRVHNSLLRNNDYAAILRKGGDLDVKNSII
                     LASGDRRYVYWGGGGTLSSDYNNIRATDGAFVSGNEDRLPDRFLIDWQIRTSFSNDSR
                     SLGYEPLFANAGTYDFHLLSEYGRYNPINGSWTNDTSTSRLIDMGAPAFAFTNEPSPN
                     GSRINIGLHGNTGEASKSPGGGALVPITVSDGGTLRGTVNLYWSWNGVSNNAPVNVQF
                     SADGGATWSNIA"
BASE COUNT         1007 a         1166 c         1401 g         1055 t
ORIGIN      
        1 ccgcttggca gtgctctgca tgatgcggat atttggatca aaaagtaatt ttttatcccg
       61 gctgcccttc agcgcgacga ttccaacccg tcggcggcgt gattcggggc tcgcagtatg
      121 ccagacagtt tcgccccgcc tgtctcgcat ccggttcaga cttgccgcgt taattttcca
      181 cgtttacacc aagggatttt gttggttccg gggatggtaa aggagataaa aaggggttgt
      241 aagtccgctt gtcagagggg agggggataa gctatattca aactatggca ctatagggcg
      301 cgagctggag cttggcgctg gtgcccagat gtgaggacga caactatgag agcaatgcgt
      361 ggcgtattgg gcctggtggc cagtttgtgc cttctggcgt ttagtagcga aacgcgcggg
      421 cagagctgga cgaacgagcc aatgccgaat ggtgggcggg tgaacgtggg cttgtatggg
      481 ggtacagccg aggccagcaa gagccggacg aatgagtggc tttttgcaat gtcgttcaat
      541 gacgggggca cactgattgg gacgagccgc gtggagtggg tgggggggaa tctggggacc
      601 aatgccacag tcaatttgca gtacacgacg aacaatgggg tgagttggga gagtattgcc
      661 accggggtga cggcaacaaa tgagagctac acttgggcgc ctgattttga ctatccggcc
      721 gtgttatggc gcgtggtgca taccaactcg ggggtggcct cgaccaatgc caaggtgttc
      781 agcgtgcggc gctccaccaa tgtggtgttc aatttttacg tgaatgacaa tagcaccgat
      841 aacgatgtgt actgttccgc actggggagt tccaccaatg acggcgcggc gtcatcgcgg
      901 ccgctgcgca gcttgcagtc ggtagtgaac cgttatcagt tgcgcggagg cgataccgtg
      961 tacgtggaca cgggtgatta tgcgacgaat gtgacgatta ctattggcgg ttttgattcg
     1021 ggggtggccg gcaagccggt caaaatcatc ggcagtccga aaggctccgt tttcaatcgc
     1081 gggaacatag gggctaatgt gctggacctc gccggtgcaa gctatttgga gattgaaaac
     1141 ctgaagctga ctggcggagc cgccggtctg aatggcgggg tgagcaatat tgtcctgcgc
     1201 gggttgcagt ttattggtaa cacgcatggt gttgtcatca atggtgccgc gcacactttt
     1261 gagaattgcg tggcggcgga taacacacag cgggccttta acggcagtgg gagcggggcg
     1321 aatgtgtggc gaaatggcgt catgtggggc agtcctacgt tggttttcgc acccaccaac
     1381 gcaacgcttt cggtatccaa cagcattctg ggcaacggga ccaccttgtt tggaagtcag
     1441 attattccgg gcgatttcaa tctggtttgg aacgtaggcg tggggctgag ctatcaggcc
     1501 tttactgatt tccaaaatgc aggatattgg accaatagtc tttatgcgga ccccgtcttt
     1561 gccaatgctg cgggggggga cttccatctt aagagcgtga tgggacggta taacccggcc
     1621 accggccttt ttgagaccaa tgatgctgtg cattcccccg gcattgactt gggcgatccc
     1681 agcgcgattt ataccaatga accggtcccc aacggctcac gcttgaatgt cggtttgcac
     1741 ggaaacacgg cccaagccag caaaagccgg acgaatgcct ggctgcaaat ccgcagctat
     1801 acggacgggg gcactcttga tgcgcaagcg ggatcatggc tgcggtggaa tggagcgaac
     1861 tttgatccca gcgcaacggt gacgttgtgg ctttcgcggg ataatggggt gagctgggag
     1921 acgctggcca ctaaccagat ggccaccaac ggcgcctatt tctatcaaaa cacttcttca
     1981 aacgactatt ccgccttgta cgccatgtgg aaagtcacgc tggacaacgc caccccaagc
     2041 gtcagcagcc agtcgcccac gaactttaac taccggaatg gggcctatgc gttttatgtg
     2101 aacgatgcct cgaccaatgg cgatgtctat tgcactgcgc cggggagcga cagcaacttg
     2161 ggcgtaagtc ccggcgcgcc catgcaatcg ctgcatacgt tgatcaatac gtataaaatt
     2221 ggcccaggag accgggtcta tattgatacg ggtgtttata ctgccacaaa taccgtgctg
     2281 attaccgcgc aagactccgg cggcgcaacg aaccctgtat tcatcatcgg cagcaccaat
     2341 cgtctcgccg gcggcagcct gttcaggtca ccagccattc cgcaacggcc gctggcgttt
     2401 aatatttcgg gtgcggcatc caacattatt ttgcgagata tagtggtcag taatgcttcg
     2461 cggggcgtaa tggtatccaa tgctctgaac atcaggctgg aaggcgtgga ggtgcgcggg
     2521 gccggacaaa ccgcatttga gctggccaac aatgcgcgtt ccgtcactgt gacccggtgt
     2581 gtggcacatg gcggaggaat cggaatctcc ttacaacagg ccacaaacat agtcgtgaac
     2641 cacagcttgt tttggcagaa cacgaataat gcggtttatc tcggcactca ggtcgggctg
     2701 ctgttggaaa acagtatttt ggcatccacc aatttcggcg ccgccctgta ctctgtgccg
     2761 agcaattccc ccacagtcac catggattat aacggaatcc atgctgggcc gaatacgcgg
     2821 gtggggatca accggacaac gggtgcgcgc gcagatagcc tggtggcatg gcagtacctg
     2881 gccaacgacc aagacgtgca tagcatcgcc aacaatcctc tcatggcgga tccggatgcg
     2941 tttgattacc atttgctaac cgaacaagcg ttgggtcgaa ttatgcccaa tggccaacgg
     3001 accacagacc cggtgtcttc gccactgctg gatgccggca acccagccag cgatttctct
     3061 gcggagccgg ccccgaatgg tgggcgtgtg aatattggtg ttttcggcgg gagcgcgcag
     3121 gcaagtagtg cccgagtcag tccatggctg aaagctattt cctttggtga tgccggctat
     3181 gtaaccaatg gaaccttcac attgcgatgg attgcgggtg gcgagttatc caatgaaaca
     3241 gtcaaggtgg aggtgtcggt tgatggcggg cgctcctgga cccaaacggt ggcctccggg
     3301 gtggctgcga ccaatggcga ggctacttgg aacgtggccg acttgccaga cacacccgcg
     3361 ggggtgtggc gggtgaccgg cctgcaacga accaatgttt ggagtggctc ttccaacttc
     3421 tttgctatcc gcaaccaacc cttgaatctg tatgtgaatg gcatttatac caacgaggcc
     3481 gtgtacacga cggcagccgg tcggccggac aattggatgg ccacatcaaa cgcgccgctg
     3541 gattcgttgc gcgtcgtgtt tgaacgattt gaccttgagc ctggcgatag catttgggtg
     3601 gatccggcgg tctatctgga acccgctgcg atttcgatca gcctgaaaaa ttccggcacc
     3661 accagccagc ccatccgcgt gaccggcccg ttgaataagc cctataccgg tgccattttg
     3721 gcgcgaacga gccggggctc aggaagctat gtcattcagt tggtaaatgc ccgcggaatt
     3781 gaattttctg ggctggtggt ttccaatgcc tttatcggga tttatgaaca aaacagtcag
     3841 ggaattttgt ggaacaaagg gcgcgtgagc cattgcgtga cgaacggtat tttcgctggg
     3901 tctggcgcag acctgacggt ttcacattcc attatcgaga acagcttgac ggcgggcctc
     3961 caaactttaa caggctcaac tatccgagtt cacaacagct tattgcggaa taatgattac
     4021 gcggccattc tgcgcaaggg cggtgacttg gacgttaaaa acagcattat attggcgagt
     4081 ggcgaccgcc gctatgtata ttgggggggc gggggcacct tgtcttccga ttataacaat
     4141 atccgcgcaa cggatggcgc ctttgtgtcc gggaacgagg accgactgcc agatcgcttc
     4201 ttgattgact ggcaaatccg caccagtttt tccaatgatt cgcggagctt ggggtatgag
     4261 cctctttttg cgaatgccgg gacgtacgat ttccatctgt taagcgagta tgggagatac
     4321 aatccgatta acggtagttg gacgaatgat acgtccacct cccgcctgat tgacatgggg
     4381 gcccccgctt tcgcctttac caatgagccc agccccaacg ggagtcgaat caacattgga
     4441 ctgcatggga atactggcga ggcaagtaag agccctggcg gcggtgcgtt ggttccgatt
     4501 accgtatctg atggtggcac tctgcggggc acggtaaatc tgtattggtc ttggaatggt
     4561 gtctcgaata acgcccccgt taatgtccag ttttctgccg atgggggggc gacatggtcc
     4621 aatattgcc
//
LOCUS       DAOPNP010000143         4591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_20430_length_4591_cov_10.193122, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000143 DAOPNP010000000
VERSION     DAOPNP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20430_length_4591_cov_10.193122"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /gene="tuf"
                     /locus_tag="PKI64_10050"
     CDS             <1..170
                     /gene="tuf"
                     /locus_tag="PKI64_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362418.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOE01380.1"
                     /translation="GDVTLPEGVEMVMPGDNIDMAVELITPIAMEKNMRFAIREGGRT
                     VGAGVVAEIVE"
     gene            239..391
                     /gene="rpmG"
                     /locus_tag="PKI64_10055"
     CDS             239..391
                     /gene="rpmG"
                     /locus_tag="PKI64_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOE01381.1"
                     /translation="MPREQITLACTECKRRNYVNSRNKKTKTERLELKKYCPAERKHT
                     VHREAK"
     gene            429..504
                     /locus_tag="PKI64_10060"
     tRNA            429..504
                     /locus_tag="PKI64_10060"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:462..464,aa:Trp,seq:cca)
     gene            559..756
                     /gene="secE"
                     /locus_tag="PKI64_10065"
     CDS             559..756
                     /gene="secE"
                     /locus_tag="PKI64_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOE01382.1"
                     /translation="MSKIAGSIRGIRVFFDEVGAELKKCAWPNRAELFDSTIVVVVSV
                     GLLGLFVALCDVLLRELMKLV"
     gene            776..1327
                     /gene="nusG"
                     /locus_tag="PKI64_10070"
     CDS             776..1327
                     /gene="nusG"
                     /locus_tag="PKI64_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOE01383.1"
                     /translation="MDKKWYVLHVLTGQEAKVRENLLKRVQMEEMGDYVGEVLIPTEK
                     VSEVKQGKRTTQVRKFFPGYVLVEIAMYDENKQLLEKPWAFIQATPGQIGFIGGKRPV
                     ALRPDEVANILQQIEEKKERVKPKVVFEPGETVRITDGPFLSFGGIVEEVDPERGKLK
                     VSVSIFGRTAPVELEYWQVERGE"
     gene            1341..1763
                     /gene="rplK"
                     /locus_tag="PKI64_10075"
     CDS             1341..1763
                     /gene="rplK"
                     /locus_tag="PKI64_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOE01384.1"
                     /translation="MAKKITGIIKLQIPAGQANPAPPVGPALGQQGVNIMAFCKEFNA
                     ATQKEAGMVIPVVITVYQDKSFTFITKSPPAAALLKKAAGIAKGSGVPNRDKVGKVSK
                     KQLVEIAKLKAKDLNANDDDHAIRMVAGTARSMGIEVE"
     gene            1773..2465
                     /gene="rplA"
                     /locus_tag="PKI64_10080"
     CDS             1773..2465
                     /gene="rplA"
                     /locus_tag="PKI64_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOE01385.1"
                     /translation="MAFHGKKYKKVAAKVDRETLYTIEGALKFLKDNPTTKFDATAEI
                     AFRLGVDPTKSDQAVRSTVTLPFGTGKTVRVLVFASGPAAEAAREAGAEYVGFEDMIA
                     KVTGGFTDFDVAVATPDAMKEVRKLGKVLGPRGLMPNPKTGTVSDDTAAAVKACKAGR
                     VEFRMDRHGNVMVPFGKMSFDLPALVGNCKAVIDGLNAARPAAAKGIFIKRATITSTM
                     GPGLRLDPRETA"
     gene            2491..3009
                     /gene="rplJ"
                     /locus_tag="PKI64_10085"
     CDS             2491..3009
                     /gene="rplJ"
                     /locus_tag="PKI64_10085"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOE01386.1"
                     /translation="MRAEKIAIAREIKEQLGAAKFAILTDFTRMDTAKTMALRRKLRE
                     VEARYQVVPNRLFRKVAQELELTGIEAGLKGPTAIVFGDGDVAAAAKALRDFIKAEKV
                     PVVKLGHMDGAVLQAADVETLASLPSKKVMQGILVGTIAAPMSNLVGVMSQKLASLVY
                     VLKAAADKKSAA"
     gene            3097..3468
                     /gene="rplL"
                     /locus_tag="PKI64_10090"
     CDS             3097..3468
                     /gene="rplL"
                     /locus_tag="PKI64_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOE01387.1"
                     /translation="MAEFVDWIETISVLELSQLVKALESRLGVSAAAPVAVAAAPAAG
                     GAGAAAAEEKTEFTVELTDAGAQKIAVIKEVRAITGLGLKEAKDLVEGAPKAVKENVA
                     KDEAEKIKKQLEAAGAKITIK"
     gene            3577..>4591
                     /gene="rpoB"
                     /locus_tag="PKI64_10095"
     CDS             3577..>4591
                     /gene="rpoB"
                     /locus_tag="PKI64_10095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOE01388.1"
                     /translation="MVDRINFGKLTDVVELPDLIEIQTRSYLEFLQMDVAPTKRRLVG
                     LQAVFKEVFPIESYDGNCSLDFVKYEITPPKQTAFASLKEGESHTAALHVTFRLRNQQ
                     EVREETVFMGEVPLITEQGTFIINGAERVIVSQLHRSPGICFEQTLHANGTLLHSFRV
                     IPDRGSWVEVQFDTSDMLNIYLDRKKRRRKFLATTFLRALGYGTDEELLGLFYKIEKL
                     DVAKPRAEQLDNRVFLADVLDDTRVIVKKFEPVTRDMLNEVKSAGIATVDVVDVTWDE
                     GLLLKSMRKDQTASTDDALKDIYTKLRPGDPATTSNARQLLKRIFFDPRRYDLGRVGR
                     YKIQ"
BASE COUNT         1017 a         1279 c         1513 g          782 t
ORIGIN      
        1 ccggcgacgt gaccctgccg gaaggcgtcg aaatggtgat gccgggcgac aacattgaca
       61 tggctgtgga gctgattacg cccatcgcca tggaaaagaa catgcgcttt gccattcgcg
      121 aaggcggccg cacggtcggc gccggcgtgg tggcggagat cgtggaataa ggttcgcggc
      181 cgtcttgggg cgcccgcgcg tgcggcgcgc cccgccagcc gcaacgggag tttgagccat
      241 gcccagagaa cagatcacgc tggcgtgcac cgagtgcaag cgccgcaact atgtcaacag
      301 ccggaacaag aaaaccaaga ctgaacgctt ggaactgaag aagtattgtc cggcggagcg
      361 gaagcacacg gtgcaccgcg aggcaaagta gggcagggcg gaactcgttt cacggcaggc
      421 gcaccgacag gccagtagct caattggcag agcaccggtc tccaaaaccg gttgttgggg
      481 gttcaagtcc ctcctggcct gccaagtccg caaaaggccc gcaggcgggt cgccgctaac
      541 gaaaaggcag agcaaacaat gagcaagata gcaggaagca tccgaggcat ccgcgtgttt
      601 ttcgacgagg tcggcgccga gttgaaaaag tgcgcctggc cgaaccgcgc cgagctgttc
      661 gattcgacca tcgtcgtggt ggtttccgtg ggcctgctgg gcttgttcgt ggcgctgtgc
      721 gacgtgctgc tgcgcgaact gatgaagctg gtctaagccg gggagcaagc agcccatgga
      781 caaaaaatgg tatgtgttgc acgtcctgac cggacaggag gcgaaggtgc gggaaaacct
      841 gctcaagcgc gtccagatgg aagagatggg cgactatgtg ggcgaggtgc tgatccccac
      901 ggaaaaagtt tccgaagtca agcagggcaa gcgcaccacg caggtgcgga agtttttccc
      961 gggctatgtg ctcgtggaaa ttgcgatgta cgacgaaaac aagcagttgc tggaaaagcc
     1021 gtgggcattc atccaggcca cgccgggcca gattgggttc atcggcggca agcggccggt
     1081 ggccttgcgc ccggacgaag tggccaacat cctgcagcag atcgaagaga agaaggaacg
     1141 ggtcaagccc aaggtggtgt ttgagccggg tgagacggtc cggattacgg acggcccctt
     1201 cctgagtttt ggcggcatcg tggaagaagt ggacccggag cgcggcaagt tgaaggtctc
     1261 ggtttcgatc ttcggccgca cggcgccggt ggaattggaa tattggcagg tggagagagg
     1321 cgaataggcc cgggtgtttt atggcaaaga aaattacagg catcatcaaa ttgcagattc
     1381 cggcgggcca ggccaatccg gcgcccccgg tcgggccggc tttgggccag cagggcgtca
     1441 acatcatggc gttttgcaag gaattcaacg ccgcgaccca gaaggaagcc gggatggtca
     1501 ttccggtggt catcacggtt tatcaggaca agagctttac gttcatcacc aagagtccgc
     1561 cggcagcggc gctgctgaag aaggcggccg gaattgccaa gggctcgggc gtgccgaacc
     1621 gcgacaaggt gggcaaggtc agcaagaagc agttggtgga gattgccaag ctcaaggcca
     1681 aggatttgaa tgcgaacgac gacgaccacg ccatccggat ggtggccggc accgcgcgca
     1741 gcatgggaat tgaagtggag taaggtgcag ccatggcatt tcacggaaag aaatataaaa
     1801 aagtggcggc caaggtggac cgcgagacgc tctacacgat tgaaggcgcg ctgaagttcc
     1861 tcaaggacaa tccgaccaca aagtttgatg ccacggccga gatcgccttc cggctgggcg
     1921 tggacccgac caagagcgac caggctgtgc gcagcaccgt gacgctgccc ttcggcacgg
     1981 gcaagaccgt gcgcgtgctg gtgttcgcca gcggcccggc ggcggaagct gcccgggaag
     2041 cgggcgcgga atatgtcggt tttgaagaca tgattgccaa ggtcaccggc ggctttaccg
     2101 atttcgatgt ggccgtggcc acgccggacg cgatgaagga agtccgtaag ctgggcaaag
     2161 tgctcgggcc gcgcggcctg atgccgaacc ccaaaaccgg cactgtcagc gatgacaccg
     2221 ccgcggcggt caaggcctgc aaggccggcc gcgtggagtt ccgcatggac cgccacggca
     2281 acgtgatggt gccgtttggc aagatgtcgt ttgacctgcc ggcgctggtg ggcaactgca
     2341 aggccgtgat tgacgggctg aacgccgccc gcccggcggc cgccaagggc atttttatca
     2401 agcgcgccac gattacctcc accatgggcc ccggcctgcg cctggacccg cgcgaaaccg
     2461 cctaaccctt tctcgtacgg agagccgacc atgagagctg aaaagattgc gattgcccgg
     2521 gaaatcaagg agcaactggg ggccgccaag tttgccatcc tgaccgattt cacccggatg
     2581 gataccgcca agacgatggc cttgcgccgc aagctgcgcg aggtggaagc gcgttatcag
     2641 gtggtgccca accgcttgtt ccgcaaggtg gcgcaggaac tggaattgac gggcatcgag
     2701 gccgggctga agggcccgac ggccattgtg ttcggcgatg gcgacgtggc cgcggccgcc
     2761 aaggcgctgc gcgacttcat caaggccgag aaggtgcccg tggtgaaact gggccacatg
     2821 gatggcgcgg tactgcaagc cgccgatgtc gaaacgctgg cttccctgcc gtccaagaag
     2881 gtcatgcagg gcatcttggt gggcacgatt gcggccccga tgagcaattt ggttggtgtg
     2941 atgagccaaa aactggcgag cttggtctat gtgctgaagg ccgccgccga caagaagagc
     3001 gccgcctaag cacagggtga gcgcccgtaa tcacgattta aaaaacaagg atcaggagac
     3061 atcccaatga acgaagaagt aaaactcgaa ggaaaaatgg ctgaatttgt ggactggatc
     3121 gagaccattt cggtcttgga actttcgcag ttggtgaagg cgctggaatc ccgcttgggc
     3181 gtttcggcgg cggctccggt ggcggtggcc gcggctccgg ccgcgggcgg cgctggcgcg
     3241 gcggcggcgg aagaaaagac cgagttcacc gtggaactga ccgatgccgg cgcgcagaag
     3301 attgccgtca tcaaggaagt ccgcgcgatc acgggtctgg gcctgaagga agccaaggat
     3361 ctggtggaag gcgccccgaa ggcggtcaag gaaaacgttg ccaaggacga agccgaaaag
     3421 atcaaaaagc agcttgaagc tgccggtgcc aaaatcacca tcaagtagcc cctccaaggt
     3481 agtgggacag ccgccccggg acgctgaaaa gcgcatccgg ggtgtgcctg tcttttggtt
     3541 tgcgcgattt ttcccctggc atgaaaggcg acacggatgg ttgacagaat taattttggc
     3601 aagttgacgg acgtggtcga gttgccggat ctcatcgaga tccagacccg ctcctatttg
     3661 gaattcttgc agatggatgt ggcgcccacc aagcgccgct tggtcggttt gcaggcggtt
     3721 ttcaaggaag tattcccgat tgaaagctac gacgggaact gcagcctcga cttcgtgaag
     3781 tatgaaatca cgccgcccaa acagacggcg tttgccagct tgaaggaagg cgaatcgcac
     3841 acggcggcgc tgcacgtgac cttccgtctg cgcaatcagc aggaagtccg cgaagaaacg
     3901 gtgttcatgg gcgaagtgcc gctgatcacc gagcaaggca cgttcatcat caacggcgcg
     3961 gagcgcgtca ttgtcagcca gttgcaccgt tcgccgggca tctgctttga gcagaccctg
     4021 cacgccaacg gcacgctgct gcattcgttc cgcgtgattc cggaccgtgg ctcgtgggtg
     4081 gaagtgcagt tcgacacctc ggacatgctc aacatctacc tcgaccgcaa aaaacgccgc
     4141 cgcaagtttt tggcaaccac gttcctccgc gccctcggct acggaaccga tgaagagctg
     4201 ctgggcctgt tctacaagat cgaaaagctg gacgtggcca agccgcgcgc cgaacaactg
     4261 gacaaccgcg tgttcctggc cgacgtgctg gacgacaccc gcgtgatcgt caagaaattc
     4321 gagcccgtca cccgcgacat gctcaacgaa gtgaagagcg cgggcatcgc caccgtggat
     4381 gtggtggatg tgacctggga cgaaggcctg ctgctgaaga gcatgcgcaa ggaccagacg
     4441 gcctccaccg acgatgcgct caaggatatc tataccaagc tgcgccccgg cgatccggcc
     4501 accacctcga atgcccggca gttgctcaaa cgcattttct tcgatccgcg ccgctatgat
     4561 ctcggccgcg tgggccgcta caagatccag c
//
LOCUS       DAOPNP010000144         4549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_20668_length_4549_cov_8.556742, whole genome shotgun sequence.
ACCESSION   DAOPNP010000144 DAOPNP010000000
VERSION     DAOPNP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20668_length_4549_cov_8.556742"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKI64_10100"
     CDS             <1..202
                     /locus_tag="PKI64_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01389.1"
                     /translation="REKNKNMIDGHPQRSAFLFGFIFAFLRLLHLLRLLRCGFMVFRA
                     PVFLPPSKTCCVPIYTGVYIGA"
     gene            199..558
                     /locus_tag="PKI64_10105"
     CDS             199..558
                     /locus_tag="PKI64_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE01390.1"
                     /translation="MKLHVRIALATARDEEFCGAGVLELLAGIARHGSIQQAARDMGL
                     SYVKALRILNQTEKELGLALLERRKGGAQRGSTALTPTARRFVRDFTRLRATIQKSAQ
                     QAFRPFQRVYERKKETR"
     gene            555..2540
                     /locus_tag="PKI64_10110"
     CDS             555..2540
                     /locus_tag="PKI64_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE01391.1"
                     /translation="MKAYPIGLIMGALLAGALAVSGQGTSGQSVVSLGEVVVVGMTEP
                     AGVGTATEVQGDQAELRNRRDLAGALALAPGVTLTRVGGRNEIMAAVRGFDTRQVPLF
                     IDGIPIYVPYDGNVDMGRLDVFSASELTLAKGYSSVLYGPNALGGAINVLSRRPSQPE
                     ELDVRAGATSGKGWESAVRAGLLRQNGYALAGVSWRERDYFRLSDDFKPVAAENGGAR
                     ENSDSRDIQIMAKAGWTPGGGEDELAVGFVRQDSEKGLPPYAGHDPRVLPRYWRYTDW
                     IKNSVYAVGHKTIGQTGYLKPRIYYDTYENTVKAFDDDTYSTQTKRSSWTSVYDDYSF
                     GGSLEGGGQIGERHQLGVAAHYKQDVHREHNVGDPWNTIKDETWALAGEDHISLTEWL
                     SLSAGLGVEGRHSLKARDAETGDAFPHNDNLAVNPQAGLFWAVTGGVVRATVAHKTRF
                     PTLKDRYSYRLGTALPNPDLDPERALHYEVGYDGRVAERVRGRASIFYSRLDDTIQRV
                     NHVVQDEEGAWLYQLQNVGKAEQVGAEIGATWQLVDGLAGGVDYLYMHRKNLDQSDLK
                     PADIPEHTAKFHLEWAAFSRLTLVPSVEYSSSRYSLSNSTKVDGYWLCHLDARLKLTH
                     GVELYAGIHNLLDENYELDEGYPEEGRNFYVGAQYRF"
     gene            2634..3428
                     /locus_tag="PKI64_10115"
     CDS             2634..3428
                     /locus_tag="PKI64_10115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE01392.1"
                     /translation="MAVDFAGMYREQCRRSGYGQKSSRHWDQRAASRDKRAPDGIYTE
                     ACLSRMDFTGVQTVLDIGCGTGNLAIPLARRVRHVHALDFSPEMLAHLQAHRRQAGIK
                     NLTVHQLSWADSWRGVPRVDLALCSRALGVTDLRAALTKMTRHARRRCYAITHVGGSF
                     LGPDILARLGRSIVPRPDYIYAVNILYQMGLRATVDFLQTTARMPYATPEAFLDSVRW
                     RIGDLQPREITRLKQMYRQLPVQADGQRVYQHHFTWAMLGWETTPA"
     gene            3425..4444
                     /locus_tag="PKI64_10120"
     CDS             3425..4444
                     /locus_tag="PKI64_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01393.1"
                     /translation="MKPTAWAGLDRAQAVERLTALWALNEAGLGGLIHALRVPFTGVV
                     VGSTAVVLITLIAFFAEHKARALIKAMTVVLLVKAAASPHTPLPAYISVAFQGVAAAL
                     VFGVLPSIRLGALLLGLLALWQGAVQKLIVMSVLYGRSLWESVDAVGHWILNKMGQGG
                     GDLSPTGWFVFFYLGYYTVAGLITGWLAGVVPREITELLKQPPPEKPMDLPPTPLPLT
                     SRKRWWRRTPFRAGLLLLILLAVLVWASPGDTGWHRGLKVFLRASVVLLVWMGLVRPL
                     GAALFRRFRQHEQTRYGADAARIMEQFPVLKQNAAVAWQASKGAKGFRRWQRFLVEWI
                     VRGLA"
BASE COUNT          831 a         1223 c         1560 g          935 t
ORIGIN      
        1 gcgtgaaaaa aacaaaaata tgattgacgg acatcctcag aggtctgcgt tcctgtttgg
       61 ttttattttc gcttttttgc gtctcctgca ccttttgcgt ctcttgcgtt gtggttttat
      121 ggttttccgg gctcctgttt ttttaccgcc gtcaaaaacc tgttgcgtgc cgatatatac
      181 aggtgtatat atcggggcat gaagttgcat gtgcggattg cgttggccac ggcgcgggat
      241 gaagagttct gcggcgcggg ggtgctggag ttgctggcgg gaattgcgcg gcatggctcg
      301 attcagcagg cggcgcggga catggggctc tcttatgtga aagccctgcg catcctgaat
      361 caaacggaaa aagaactggg gctggcgctg ctggaacggc gcaagggggg ggcgcaacgc
      421 ggcagcaccg ccttgacccc gaccgcgcgt cggtttgtcc gcgactttac ccgcctgcgc
      481 gccaccatcc agaagtcggc gcaacaggcg ttccggccat ttcagcgagt ttatgaacgc
      541 aaaaaggaga cacgatgaag gcatatccaa ttggactaat catgggggcg ttgctggcgg
      601 gcgcgctggc ggtgagcggc caaggcacct ccggccaatc ggtggtgtcg ctgggcgaag
      661 tggtggtggt gggcatgacc gagccggcgg gcgtcggcac ggccactgaa gtgcaaggtg
      721 atcaggccga gttgcggaac cgtcgcgact tggccggcgc gttggcgttg gcgccggggg
      781 tgacgttgac ccgcgtgggc gggcgtaacg aaatcatggc ggcggtgcgc ggtttcgata
      841 cacggcaagt gccgctgttc attgacggca tccccattta tgtgccctac gacggcaatg
      901 tggacatggg ccgcttggat gtgtttagcg cttcggagct gacgctggcc aaggggtata
      961 gctcggtgct gtacgggccg aatgcgctgg gcggcgcgat caatgtgttg agccgccggc
     1021 cctcccagcc ggaagagttg gatgtgcggg ctggcgccac cagcggcaag gggtgggaga
     1081 gcgccgtgcg ggccgggctg ctgcggcaga atggctacgc cttggcgggc gtgtcgtggc
     1141 gcgagcggga ttatttccgg ctgtcggatg acttcaaacc ggtggcggct gagaacggcg
     1201 gtgcgcgcga aaactcggac agccgtgaca tccaaatcat ggccaaggcg ggctggaccc
     1261 cgggcggggg tgaggatgag ctggcagttg gctttgtccg gcaggatagt gaaaaagggt
     1321 tgccgcccta tgccgggcac gacccgcgcg tgcttccgcg ttattggcgc tacacggatt
     1381 ggatcaaaaa cagcgtctat gccgtcgggc acaaaacgat tggccaaacg ggctacctca
     1441 agccgcggat ctattacgac acctacgaaa acaccgtgaa ggcgtttgac gacgatacct
     1501 actccacgca gaccaagcgt tcctcgtgga cgagcgttta tgacgactat tcgtttggcg
     1561 gttcgctgga ggggggcgga cagattggcg agcggcatca gttgggcgtg gccgcgcact
     1621 acaagcagga tgttcaccgt gaacacaacg tgggcgaccc ttggaatacc atcaaggatg
     1681 aaacatgggc cttggcgggc gaggatcata tctcgctgac agaatggctg tcgctgtcgg
     1741 ccggactggg tgtggaaggc cggcacagcc tgaaggcgcg ggatgccgaa accggcgatg
     1801 cgttcccgca caacgacaac ctcgcggtga acccgcaggc gggcttgttc tgggccgtga
     1861 cggggggcgt cgtccgggcc acggtcgccc acaagacccg gttcccgacg ttgaaagacc
     1921 gctattcata ccgcttgggc acggcgctgc ccaatccgga tttggacccg gagcgcgcac
     1981 tgcattatga agtgggctat gacggacgag tggccgagcg ggtgcgtggc cgggccagta
     2041 ttttttatag ccggctggat gataccattc agcgcgtgaa tcatgtggtg caggatgagg
     2101 aaggcgcgtg gctgtaccag ttgcagaatg ttggcaaggc ggaacaggtc ggcgcagaaa
     2161 tcggcgctac ctggcaattg gtggatggcc tcgccggcgg cgtggattat ctctacatgc
     2221 accgcaaaaa cttggaccaa tccgacctca aaccggcgga cattcctgag cataccgcca
     2281 agttccatct ggaatgggcc gcgttttccc ggctgacgct ggtgccgtcc gtggagtatt
     2341 cttcttcgcg ctattcgctg agcaacagca caaaggtgga cggttactgg ctgtgccatc
     2401 tggatgcgcg cctgaagctg acgcacggcg tcgaactcta tgccggcatc cacaatctgc
     2461 tggatgaaaa ctacgaattg gacgaaggct atcccgaaga aggccgcaac ttttatgtcg
     2521 gcgcgcagta tcgtttttga tggctgcaca tttcagccgg agggtgtact tttgatgacc
     2581 tgatcatgcc tcaaaagaga aaaagaagtg ctgcgtcggc tgcggatccg atcatggcgg
     2641 ttgattttgc cgggatgtac cgggagcagt gtcggcgctc cggctacggc cagaaatcga
     2701 gccggcactg ggatcaacgc gccgcatcgc gtgacaagcg cgcaccggac ggcatctata
     2761 ccgaggcgtg tctctcgcgc atggacttca ccggggtgca gacggttttg gacatcggct
     2821 gcggcacggg caatctggcc atcccattgg cccggcgcgt gcgccacgtt cacgcgctgg
     2881 atttttcgcc cgaaatgctg gcgcatctgc aagcccaccg ccggcaggcg ggcatcaaaa
     2941 atctcaccgt gcaccagttg tcttgggcgg attcatggcg cggggtgccg cgcgtggatt
     3001 tagcgctgtg ctcccgggcg ctgggggtga cggatttgcg cgcggcgctc accaaaatga
     3061 cacggcacgc gcgccgccgg tgctacgcca tcacgcatgt gggtggcagc tttctcggcc
     3121 cggatatttt ggcgcggctg ggccgatcca ttgtgccgcg cccggactac atttacgcgg
     3181 tgaacattct ctaccaaatg ggactgcggg ccaccgtgga tttcctgcaa acgaccgccc
     3241 gaatgccgta tgccacgccg gaggcatttc tggattccgt gcgctggcgg attggcgact
     3301 tgcagccgcg agaaatcaca cgcctgaaac agatgtaccg ccaactgccg gtacaggcgg
     3361 acggccagcg ggtgtatcag catcatttta cttgggcgat gctggggtgg gaaacaacac
     3421 cggcatgaag cccacggcat gggcgggatt ggatcgcgca caggcggtgg agcggctcac
     3481 cgcgctgtgg gcgctgaacg aggcgggtct cggggggctg attcatgccc tgcgcgtgcc
     3541 gttcacaggc gtggtggttg gctccacggc ggtggtgttg ataacgctga ttgccttttt
     3601 tgcggagcat aaggcccgcg ctttgatcaa ggccatgacg gtggtgttgc tggtgaaagc
     3661 agccgccagt ccgcacacgc cgctaccggc ctatatttcg gtggccttcc aaggggtggc
     3721 ggcagcgctg gtattcggtg tgctgccctc catccggctc ggcgcactgc tgctggggct
     3781 gctggcgctg tggcagggcg cggtgcaaaa gctgattgtg atgagcgtgc tctacggccg
     3841 ttcgctgtgg gagtccgtgg atgcagtcgg gcattggatt ttgaacaaaa tggggcaagg
     3901 gggaggcgac ctatcgccaa ccggttggtt tgtatttttt tatttgggtt attacaccgt
     3961 ggcggggctg atcaccggat ggctggccgg tgtggtgccg cgggaaatta cggagttgct
     4021 caaacagccg ccgccggaaa aaccgatgga tttgccgcca acgcctttac cgctgacttc
     4081 gcgcaaacgg tggtggcggc gcacgccatt ccgtgccggt ctcttgttgc tgatcttgtt
     4141 ggcggtactg gtctgggcgt cgccgggcga caccggctgg catcgcggtc ttaaggtttt
     4201 tctacgtgcc agcgtagtgc tgttggtctg gatggggctg gtgcgcccgc tgggggccgc
     4261 gctgtttcgg cgttttcgac agcatgaaca aacccgttat ggcgcggatg cggcacggat
     4321 catggaacaa ttcccggtgt taaaacaaaa tgcggcggta gcatggcagg ccagcaaagg
     4381 ggcaaaaggg tttcgccgct ggcaacggtt tctggtggaa tggattgtcc gcgggctggc
     4441 gtgaggcggg cggtcaggca atggccgtgt agcccagcga aaccttccta ctgtgaacgg
     4501 ctggaaggtc tgcgaccgtt ccagcccttc tatgggggcc gtcaagtca
//
LOCUS       DAOPNP010000145         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_20702_length_4542_cov_7.074437, whole genome shotgun sequence.
ACCESSION   DAOPNP010000145 DAOPNP010000000
VERSION     DAOPNP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20702_length_4542_cov_7.074437"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="PKI64_10125"
     CDS             <1..1623
                     /locus_tag="PKI64_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOE01394.1"
                     /translation="GISDLRDESDKDGIRVVVELKKGEIGQVVLNNLYKQTQLGGTFG
                     AIMLAIDHGRPRVMDLKELLQCFIDHRFEVLTRRTQFELREAEARAHILEGLLKALDH
                     LDDVVRIIRAAENREAAKIQLMATFGFTEIQANAILDMRLYQLTGLERSKLEAEYKAL
                     QERIAYLKELLADARKLFGVMKDDLLAIRKEYADPRRTEIVVDEGDIDIKDLVADEDC
                     VISLSHAGYIKRTPTSVYREQKRGGKGVVGMDTKDEDYVEHVFNCSAHDWMLFFTEKG
                     RMYFKMAYEIPESARDSRGKALVNLLDMQGSEKIAAIIPVRGFDTDKHLFFATERGIV
                     KKTPLAAYQNVRAAGIIAMNIAEDDRLIGVELTSGQDDLLLVTRQGQGIRFKETDARP
                     MGRATGGVRGISLGKGDVVEGIEVVHEDAMLLTVTENGFGKRTPFSEYRVQKRGGKGL
                     IANELTAKTGELVTALSVHDHEAIMIVTRKGTMIRMPVDGVRVTGRSAQGVKLIDLSE
                     EDVVMSATPVEPEDENGQAAPAAPPAEQEPTS"
     gene            1634..2386
                     /locus_tag="PKI64_10130"
     CDS             1634..2386
                     /locus_tag="PKI64_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01395.1"
                     /translation="MNKIVGGLLLVLLALPASWARDNSVEDRSADEALLTWANPFPWQ
                     VGAAYQNHARPVDLGGAEWKLKGQSVAGEVGVRPWPWLLVYGQVSANEARLAERMREN
                     ADPGAGGLLGVKANLWQINEGLARAAWRVRLQLAGYYAYRTSSDAGEGKLSWGEATIM
                     LPLNYHLTLARSARNVFAGEFHSLEIFAGPAFSKLDGTWTVGDWQRDFEESQDYGVVG
                     GANLWLLENLCFGASAAWFDGTSFDLSVTYRF"
     gene            2398..3285
                     /gene="folD"
                     /locus_tag="PKI64_10135"
     CDS             2398..3285
                     /gene="folD"
                     /locus_tag="PKI64_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOE01396.1"
                     /translation="MSARILDGKSLAADIRREIADDAAKLHAATGLAPGLGVVLAGDH
                     PASRSYVTAKEKACAAAGIRSRELTLPAHASRQDILAAVRDLNQDATCDGILVQLPLP
                     DAASEREVIEAIDPAKDVDGFHPVNVGKLVLGLPTFVPCTPQGIVELLRRAAIPLAGA
                     HVVVIGRSSIVGRPLSILLSQKGVDATVTLCHTRTPDVARFTQQADIVIVAAGRPNTL
                     RAEMIRPGAVVVDVGVNRVPDSSRATGYRLVGDVDFEPVAAKAAAITPVPGGVGPLTI
                     AMLLRNTVEAARRQRHPPA"
     gene            3282..4406
                     /locus_tag="PKI64_10140"
     CDS             3282..4406
                     /locus_tag="PKI64_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOE01397.1"
                     /translation="MIRVVNRYLLGDFVKSFVITLVVLTFVMYVGAVVQAIDYMSRGI
                     SGPLILKIFALNIPFTLSFVIPMSVLTTVLLHFGRLSADGESTALKACGVSLWQMAAP
                     ILLGAVVLSGICLYINAELAPRSHFIRRQMLRDLGEEDPLALLEEGRFVRDFPGMQIY
                     IGKKNGGRLEDITIMQFGDKGPKAQVYADSGEVNFNRETRVMDIVLYKVRLIEFDKTD
                     PSDINKQREITADRYPITADLRQMLQKRKVMPKPASMTFTQLIQGAANIRQVFPDLQE
                     KNVPRMRTKLAVEASKRMALSLSCFSFALLAIPLGLRSHRKESSIGIGLALLLLFLFY
                     LFIVVAESLLDRPQWRPDLIPWIPVVGSQILGTWLLYRNR"
     gene            4462..4538
                     /locus_tag="PKI64_10145"
     tRNA            4462..4538
                     /locus_tag="PKI64_10145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4496..4498,aa:Arg,seq:ccg)
BASE COUNT          887 a         1353 c         1421 g          881 t
ORIGIN      
        1 ggcatcagcg acctgcgcga cgagtccgac aaggacggca tccgcgtggt ggtggagctc
       61 aagaagggcg aaatcgggca ggtggtgctg aacaacctgt acaagcaaac gcaactgggc
      121 ggcacgtttg gcgccatcat gctggccatt gaccacggac gcccgcgggt catggacctc
      181 aaggagcttt tgcagtgctt cattgaccac cgcttcgagg tgctgacccg ccgcacgcag
      241 tttgaactgc gcgaggccga agcccgcgcc cacattctgg aagggctgct gaaggcgctg
      301 gatcacctcg atgacgtggt gcgcatcatc cgcgccgcgg aaaaccgcga ggccgccaaa
      361 attcaattga tggccacgtt tggcttcacg gaaatccaag ccaacgccat ccttgatatg
      421 cgcctctacc aactgaccgg gttggaacgc agcaaactgg aggcggaata taaggctttg
      481 caagagcgca ttgcctacct gaaggagctg ctcgccgatg cccgcaagct gttcggcgtg
      541 atgaaggacg acctgctggc catccgcaag gaatatgccg acccgcgccg caccgagatc
      601 gtggtggacg agggcgacat tgacatcaag gacttggtcg ccgacgaaga ctgcgtgatt
      661 tcgctctccc acgcgggcta tatcaagcgc acccccacca gtgtgtaccg cgagcaaaaa
      721 cgcggcggca aaggggtggt gggcatggat accaaggatg aggattacgt ggagcacgta
      781 ttcaactgtt ccgcccacga ctggatgttg ttcttcaccg aaaaagggcg gatgtatttc
      841 aagatggcct acgagattcc cgagagtgct cgcgattccc gcggcaaggc gctggtcaac
      901 ctgctggaca tgcagggctc cgagaaaatc gcggcgatca ttccggtgcg cggcttcgat
      961 accgacaagc acctgttctt tgccaccgag cgcggcattg tcaaaaagac gccgctggcg
     1021 gcctaccaga atgtgcgggc ggcaggcatc atcgccatga acatcgcgga agatgaccgg
     1081 ctgatcggcg tggagttgac cagcgggcag gatgacctgc tgctggtgac gcgccaaggg
     1141 cagggcatcc gtttcaagga aaccgatgcg cgccccatgg gccgtgccac cggcggtgtg
     1201 cgcggaatct cgctgggcaa gggtgacgtg gtcgagggca tcgaggtggt ccacgaagac
     1261 gccatgctgc tgaccgtgac cgaaaacggt ttcggcaaac gcacgccatt cagcgaatac
     1321 cgcgtgcaaa agcgcggcgg caagggcctc atcgccaacg aactcaccgc caagaccggt
     1381 gaattggtca ccgcgctgag cgtacacgac cacgaagcca tcatgattgt gacccgcaag
     1441 ggcaccatga tccggatgcc cgtggatggc gtgcgcgtga ccggccgctc cgcccaaggc
     1501 gtaaaactta tcgatttgag cgaggaggat gtggtaatga gtgccacacc agtggagccg
     1561 gaggatgaaa acggccaagc ggccccggcg gcgccaccgg cggaacagga accaacctcc
     1621 tgaaggagag cggatgaata aaattgtcgg cggattgttg ctcgtgctgc tggccctgcc
     1681 ggcatcgtgg gcccgggata atagcgtgga agaccgcagc gcggatgaag cgctgctgac
     1741 gtgggccaac ccgtttccgt ggcaggtggg ggcagcctat caaaaccacg cccgtccggt
     1801 ggatctgggc ggcgcggaat ggaagctgaa aggccagtcc gtggccggcg aagtcggcgt
     1861 ccgcccgtgg ccgtggctgc tcgtttatgg gcaggtcagc gccaacgagg cccgtttggc
     1921 cgagcgcatg cgggaaaatg ccgatccggg tgcgggtggc ctgctgggag tgaaggccaa
     1981 tctgtggcag atcaacgagg gcctggcgcg cgcggcatgg cgggtgcgtt tgcaactcgc
     2041 gggatactac gcctaccgca ccagctccga cgccggcgaa ggcaagctgt cgtggggcga
     2101 ggccaccatc atgctgccgt tgaattatca cctcaccctg gcccgcagtg cgcgcaacgt
     2161 gtttgctggc gagttccaca gtctggaaat ctttgcggga cccgcctttt ccaaactgga
     2221 tggcacatgg accgtgggcg actggcagcg tgactttgaa gaatcgcagg actacggcgt
     2281 ggtgggcggc gccaatctct ggctgctgga aaatctgtgc tttggagcca gcgcggcgtg
     2341 gttcgatggc accagcttcg atttatccgt cacgtatcgt ttttaggatg tttcgccatg
     2401 agcgctcgca ttctcgacgg taaaagcctt gcggccgata tccggcgcga gattgcggac
     2461 gatgccgcca agctgcacgc agccactggc ctcgcaccgg gactgggcgt ggtgctggcg
     2521 ggcgaccatc cggcctcgcg ctcgtacgtc accgccaaag aaaaggcctg cgcggccgcc
     2581 ggtattcgct cgcgcgagct gaccttgccc gcccacgcca gccggcagga tattttggct
     2641 gccgttcgcg acctcaacca agatgccacc tgcgatggta ttttggtcca actcccgctg
     2701 ccggatgccg ccagcgagcg ggaggtcatt gaagccattg atcccgccaa ggatgtggac
     2761 gggttccatc cggtgaatgt gggcaaattg gtgctgggtt tgccgacctt tgtgccgtgt
     2821 actccgcaag gcatcgtgga gttgctgcgc cgcgcggcaa ttccgttggc gggcgcccac
     2881 gtggtggtga ttggccgtag ctccatcgtg gggcggccgc tatccattct cctctcgcaa
     2941 aaaggagtgg atgccacggt gaccctctgc catacgcgca ctcctgacgt ggcgcggttc
     3001 acccaacagg cggatatcgt gattgtggcc gccggccggc ccaacacgct gcgtgcagag
     3061 atgattcgtc ccggtgcagt ggtggtggat gtgggcgtca accgcgtacc ggactcgtcg
     3121 cgcgccaccg gctaccggtt ggtgggcgat gtggattttg aacccgtggc ggccaaagcg
     3181 gcggccatca cccccgtgcc cggcggcgta ggcccgctga ccatcgccat gctgctacgc
     3241 aacaccgttg aggccgcgcg ccgccaacgt caccctcctg catgatccgc gttgtcaatc
     3301 gctacctgct cggcgacttc gtcaaaagtt tcgtcatcac gctggtggtc ttgaccttcg
     3361 tgatgtatgt gggcgcggtg gtgcaagcca ttgattacat gtcccgcggc atttccggcc
     3421 cgttgattct caagattttt gcgctcaata tcccgttcac gctttcattc gtgatcccca
     3481 tgagcgtgct cacgacggta ctgctgcact ttggccggct ctcggccgat ggcgaaagca
     3541 ccgcgctgaa agcgtgcgga gtcagcctct ggcagatggc tgcacccatt ttactgggcg
     3601 cagtggtgtt gtccggcatt tgcctgtata tcaacgccga actggccccg cgcagccatt
     3661 tcattcgccg acaaatgctg cgcgatctcg gcgaagaaga cccgctggct ttgctggaag
     3721 aaggacgctt tgtccgcgac ttccccggca tgcagattta tatcggcaaa aaaaatggcg
     3781 gtcggttgga agacatcacc atcatgcagt ttggcgacaa aggccccaag gcccaagtct
     3841 atgccgacag tggtgaggtc aacttcaatc gggaaacccg cgtcatggat attgtgctct
     3901 acaaggtccg cctgattgaa ttcgacaaga cggacccctc cgatatcaac aaacagcggg
     3961 aaattacggc cgaccgctac cccattaccg cggatttgcg gcagatgctg caaaagcgca
     4021 aagtcatgcc caagcccgcc tccatgacgt ttacccaact gatccagggc gcggccaata
     4081 tccggcaggt attccccgat cttcaggaaa aaaatgtgcc gcgaatgcgc accaagctgg
     4141 ccgtggaagc cagcaagcgc atggccttgt cgctctcctg tttttcattt gcgctgctgg
     4201 ccattccgct gggtttgcgc tcgcaccgca aggaatcctc catcggtatc ggtttggcgc
     4261 tcttgctgct ctttttgttc tacctgttca tcgtggtggc tgagtccctg ctggatcgcc
     4321 cccaatggcg ccccgatttg atcccgtgga ttccagttgt cggcagtcaa atcctgggca
     4381 catggctgct ctaccgcaac cgctaggttt tttcgcttgc cgcgggcaaa accgccccgt
     4441 agtgttcgcg gggttgagtg cgtgcctgta gctcaattgg acagagcgtt agcctccgga
     4501 gctaaaggtt gtgggttcga cccccgccag gcacaccatg ac
//
LOCUS       DAOPNP010000146         4497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_20976_length_4497_cov_8.377082, whole genome shotgun sequence.
ACCESSION   DAOPNP010000146 DAOPNP010000000
VERSION     DAOPNP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20976_length_4497_cov_8.377082"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..555
                     /gene="rplS"
                     /locus_tag="PKI64_10150"
     CDS             196..555
                     /gene="rplS"
                     /locus_tag="PKI64_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOE01398.1"
                     /translation="MKTALLEKVEQSQLKEKAPQFRIGDSVRVHVKIKEGDKERVQVY
                     AGTVIARDGGGANETFTVRRISFGEGVERVFPLHSPYVTKVEVERRGATRRAKLYYMR
                     KLTSKQSRLKEIKENQA"
     gene            581..1207
                     /locus_tag="PKI64_10155"
     CDS             581..1207
                     /locus_tag="PKI64_10155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOE01399.1"
                     /translation="MRFEQEAWAGGFLYVAGVDEAGRGPLAGPVVAAAVLFDRPFLQT
                     DAAKVLAGLDDSKKLSAARRDYFFAVLTTHPAAHCGVASASVAEIDQLNILRATHLAM
                     ARALAQLNPAPDYALVDGLPVRGLPVPHRAIVGGDAACWSIAAASVVAKVTRDRLMAT
                     LGTEYPAYGFERHKGYGTAAHLAALQRHGPCPIHRRSFAPVAQRTWKW"
     gene            1209..1649
                     /locus_tag="PKI64_10160"
     CDS             1209..1649
                     /locus_tag="PKI64_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOE01400.1"
                     /translation="MLRAWLARFWRQSTVPATHRSGAWGEELAARHLRAKGYRILGRN
                     VRFGPRRELDLVARDPQTDTLVFVEVKTRKSEDFGRPMAAVNRGKRRAFGRAVVRYLR
                     QLRQRPTRVRFDVVEVVGQPGDATPVIRHIANAFSLPPEYRLPE"
     gene            1651..2790
                     /locus_tag="PKI64_10165"
     CDS             1651..2790
                     /locus_tag="PKI64_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOE01401.1"
                     /translation="MKGRVVSGMVAGVLGMLLPAPAAVWDEWELDLSQVSEAGQEIID
                     RFAPESFREKYRFATAEELGAYFSPLQQALAGDSVEDLAAMAGQARQALPLLRESPQT
                     QAYADWLEARLDYFEMADEATHAIPDDAPSPPDIAPPTPGIPTPPPQPPVVPATPPPA
                     PTPTVAPPRPVPAPAPTPPAAPVATRPPPPPPPAPPRRPPPPPATVKARNSYVENQQV
                     WVSKLANRPVPARAGALLPDLKSAFRAAGVPEQLVWQAEAESSFNPAARSPAGAVGLF
                     QFMPATAQQMGLRLAPEDERLDAIKNARAAATYLKQLHGRFGDWQLALAAYNCGEGRV
                     AQALRNTGGKTFDDIHHNLPAETRMYVPKIAALIHLRENISLDIL"
     gene            2945..4123
                     /gene="ygeW"
                     /locus_tag="PKI64_10170"
                     /pseudo
     CDS             2945..4123
                     /gene="ygeW"
                     /locus_tag="PKI64_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
BASE COUNT          881 a         1449 c         1374 g          793 t
ORIGIN      
        1 aagattatga gtcttccgct ctgaccaact gagctaaggg cccgtgactg tggcggaata
       61 gtgccgcccg acggggtgaa aaacaagttt ttccaccttc cggcgggcca gctcggcccc
      121 aacccccttt tccacccttg acgctgcaac gtaaatccct atgatgcctg ctcgtttcaa
      181 ggaagaaggt acgagatgaa aactgccctt ttggaaaaag tcgaacagag ccaactgaag
      241 gaaaaagctc cgcagttcag gattggcgat tccgttcgcg tgcacgtgaa gatcaaggaa
      301 ggcgataagg aacgcgtgca ggtgtatgcc gggacggtga tcgcgcgcga tggcggcggg
      361 gccaatgaga cgtttaccgt gcggcggatt tcgttcggcg aaggcgtgga gcgcgtattc
      421 ccgctgcact cgccctatgt caccaaggtg gaagtggagc gccgcggcgc tacccgccgg
      481 gccaagctgt attacatgcg caagctgacc agcaagcaaa gccgcctgaa ggaaatcaaa
      541 gaaaaccagg cctgagccgc ctggagggga atcccccgtg ctgcgcttcg aacaagaagc
      601 ttgggccggg ggatttttgt atgtggcggg ggtggatgag gccgggcgcg ggccgctggc
      661 cggccccgtc gtggcggcgg cggttttgtt cgaccgtcct tttctgcaaa ccgatgccgc
      721 caaagttctg gccgggctgg atgattccaa aaaactttcc gccgcgcggc gcgattattt
      781 ttttgccgtg ctgaccacgc acccggcggc gcattgcggc gtggcttccg ccagcgtggc
      841 ggagattgac caactgaaca tcctgcgcgc gacgcatctg gccatggcgc gcgcgttggc
      901 gcaactgaat ccggcgccgg attacgcgct ggtggatggc ctgccggtgc gcggcctgcc
      961 ggtgccgcac cgcgccattg tgggcgggga tgccgcgtgc tggtccatcg cggcggccag
     1021 cgtggtggcc aaggtcactc gcgaccgcct catggcgacg ctgggcacgg agtatccggc
     1081 gtatgggttt gagcgccaca agggctacgg caccgcggcc catctggccg ccttgcaacg
     1141 gcacggcccg tgcccgattc atcgccgctc attcgcgccc gtggcccaac gcacgtggaa
     1201 gtggtgagat gttgcgggcg tggctggcga ggttttggcg gcaatccacc gtgccggcca
     1261 cgcaccggag cggggcgtgg ggcgaggagt tggctgcccg ccatttgcgc gccaagggct
     1321 accgcattct gggccgtaat gtgcgctttg gtccgcggcg cgagctggac ttggtggcgc
     1381 gcgatccgca gacggatact ttggttttcg tggaggtcaa aactcgcaag tcggaagatt
     1441 ttggccggcc catggcggcg gtgaaccgtg gcaaacggcg cgcgtttggg cgcgcggtgg
     1501 tgcgctatct gcgccaattg cgccagcggc ccacccgtgt gcgttttgac gtggttgagg
     1561 tggtcggcca acccggcgac gcaacacccg tcatccgcca tattgcgaat gcattttcgc
     1621 tgccgccgga gtatcgtttg ccggagtgac atgaagggga gagtggtcag cggaatggtc
     1681 gccggcgtgc tgggcatgct gctgcccgcc cccgcggcgg tgtgggatga gtgggagctg
     1741 gacctctcgc aagtctccga ggcggggcag gaaatcattg accgttttgc gccggaatcg
     1801 ttccgcgaaa aataccggtt cgccaccgcg gaggagctgg gcgcgtattt ctcgccgttg
     1861 cagcaggcct tggcgggcga cagcgtggag gacttggccg ccatggccgg gcaggcgcga
     1921 caggcgctgc cgcttttgcg cgagtcgccc caaacgcaag cctacgcgga ttggctggag
     1981 gcgcggctcg attattttga aatggcggac gaggccactc acgccatccc cgatgacgca
     2041 ccctctccgc ccgacatcgc gccgcccacg cccggtatcc cgacaccgcc gccgcagccg
     2101 ccggttgtcc ccgcgacccc accaccggca ccaacgccca ccgttgcgcc tccgcgcccg
     2161 gtgccagccc ccgcgccgac accgccggcg gcaccggttg ccacccgccc gcctccccct
     2221 ccaccccctg cccctccgcg ccgccccccg ccaccgcccg ccaccgtcaa ggcgcgcaac
     2281 agctacgtgg aaaaccagca ggtgtgggtg agcaagctgg ccaaccgtcc agtgcccgcg
     2341 cgtgccgggg cgttgctgcc cgacctcaaa agcgccttcc gcgctgcggg ggtgccggag
     2401 caactggtct ggcaggccga ggccgagtcc agcttcaatc cggccgcgcg cagcccggcg
     2461 ggcgccgtgg gtctgttcca attcatgccg gccaccgcgc aacagatggg cctgcgcctc
     2521 gcgccggaag atgaacgctt ggatgccatc aagaatgccc gcgccgccgc cacctatctc
     2581 aagcaactac atggccgctt cggcgattgg cagttggcac tggccgcata taactgcggc
     2641 gaaggccgcg tggcccaagc cctgcgcaac accggcggca agacgttcga cgacatccac
     2701 cacaacctgc ccgccgaaac ccggatgtat gtccccaaaa ttgccgcgct gatccacctg
     2761 cgggaaaata tcagtttgga tattttgtaa gctccactgc cgcccgactg ccgccaagcg
     2821 cagcaacgga tagagcgttt tacgccggcg cgcactttat tgccgttagg ggattgcgcc
     2881 ccccatgttt ctccgctagc atcctcctcg atttttagca accaaccaaa aggaataaaa
     2941 gaacatgaag cccatcgaga tgctcaagtc catcgagaag ttgaacgcct ccctgtacgg
     3001 caaggatttc ctgctcactt gggataagac cgtccccgaa ctcaaactga ttttgcaaac
     3061 ggccgaattg ctcaaggccc tctacgagca aaatgtgtcg ttgcgcgtgt ttgattccgg
     3121 cctcgccatc tccaacttcc gcgacaactc cacgcgcacg cgcttctcct ttgcctccgc
     3181 gtccaatctg ctcggcctga ccgtgcagga cctcgacgaa ggcaagtcgc agatcgcgca
     3241 cggcgaaacc gtccgcgaaa ccgccaacat gatttccttc ctcacccaga tcatcggcat
     3301 ccgcgatgac atgtatctcg gcgccggcca cacttacatg aaggaagtcg gcgaagctct
     3361 cgacgacggc ttccagaacg gcgtgctgcc ccagcgcccc ggtatcgtca acctgcaatg
     3421 cgacattgac caccccacgc agtccatggc ggacctcgcc tggctcaagg aacactttgg
     3481 ttcgttggaa gcgctgcgcg gcaagaagat tgccatgacc tgggcctaca gcccgtccta
     3541 cggcaagccc ctctccgtgc cgcagggcat cattggcctg atgacccgct tcggcatgga
     3601 tgttgtcctc gcccaccctg aaggctacga cctcattccg gacgtggtgg aattggccgg
     3661 caagcaagcc aaggccaccg gcggttcctt caagcaagtg acctccatgg aagaagcctt
     3721 cgccggcgcg gatattgtgt atcccaagtc ctgggcgcca tacaaggtga tggaaaagcg
     3781 cacaacgctg ctccgcgcga aagacatgaa gggcctcgac gatctcgaaa aacaatgcct
     3841 tgcccagaac gccaacttca agaactggga gtgcgacgaa gccaagatga aactcaccaa
     3901 gaagggcgag gccctctaca tgcactgcct gcccgcggac atcaccgatg tctcctgcaa
     3961 ggccggcgaa gtttccgcca gcgtgtttga gaagtaccgc attcccacct acaaggaagc
     4021 cggctacaag ccctacgtca tcgccgccat gatgttgacc aaccgcttca agaacgtcac
     4081 cggcaccttg gccgacctga tgcgttaccc gatggagcgg atgctggatg tcagcgtggc
     4141 tggccccgcc ccggtgaaaa aagccgtcag caagaagccc gcgaaaaagg tgaaaaaggc
     4201 cgtggccaag aaagccgcca agcccgccaa gaagagcgcc aagaagccgg cgaagaagaa
     4261 agccaggagc taggctgaac cgtctgggct aacccctgcc ggcttgacct gcgccgccca
     4321 cttgggcggc gcattttttt gattccacgc aattttcagt gactggagcg tcccgctggg
     4381 gatgggaaag cagccgtttt cagaccgccg tcaatttctc atttgaagca tgatgtccag
     4441 agttcactgt catcccatag cggatgaaag aaaaagctgg aatggggcga tgaaatc
//
LOCUS       DAOPNP010000147         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_21253_length_4454_cov_8.538077, whole genome shotgun sequence.
ACCESSION   DAOPNP010000147 DAOPNP010000000
VERSION     DAOPNP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21253_length_4454_cov_8.538077"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..894)
                     /locus_tag="PKI64_10175"
     CDS             complement(202..894)
                     /locus_tag="PKI64_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01402.1"
                     /translation="MLADLLQLPPADVFAHMACDEALARAQPDHFCLRFYRWQGPAAT
                     FGYAQRVREVMATLPPAVGTAYTRRPTGGGVVPHLHDLTFSCVFPSAGEMRAAVVYRR
                     LHTAIGEGLRGMGLPVELCARGGSAAPHHAGGASQCFVQPVTMDIMLAGEKVLGGAIR
                     RFGDTVLYQGSLQLADARAREVEYQAVVAEAIAKAWGLAGWELCPVPQAVRDAATALA
                     VRYRSPEWIQRR"
     gene            complement(905..2362)
                     /gene="gcvPB"
                     /locus_tag="PKI64_10180"
     CDS             complement(905..2362)
                     /gene="gcvPB"
                     /locus_tag="PKI64_10180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOE01403.1"
                     /translation="MNRGEPLSIEKSVPGRRALTFPAPSASAAAWLPGVALRDASPRL
                     PELSELDVVRHYTRLSQLNFCLDTHFYPLGSCTMKYNPRSLEEFARQDGFAAAHPTAP
                     ETAVQGTLAALHGLEQLLCQLTGLAAFTLQPAAGAHGELTGILIAAAYHRARKDRRRT
                     EIVVPDTAHGTNPASAALGGFAVKTVATQPNGRLDVAALRAALGPQTALVMLTVPNTL
                     GLFETQIREIAAAVHEPGALLYMDGANFNALLGLARPADFGVDILHLNLHKTFATPHG
                     GGGPGAGPVGVTATLEPFLPLPRVMRRKGRYALQTKGARSIGRVKSFAGNVAVALKAY
                     AYLRRHDATTLRRVAENAILNANYLRVKLRDLFPPAVDEPCLHECVLRTEPDRLNGVK
                     TLDVAKRLLDHGFYAPTIYFPLNVPEALMIEPTETETPATLDAFVAALRAIHEEARTA
                     PEQVKSAPHNLPVRRLDELNAARNPNVAWPAQPPQ"
     gene            complement(2359..3690)
                     /gene="gcvPA"
                     /locus_tag="PKI64_10185"
     CDS             complement(2359..3690)
                     /gene="gcvPA"
                     /locus_tag="PKI64_10185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOE01404.1"
                     /translation="MYSPHTAAEQQAMLRDIGVGSLADLLAQIPAELQASVTAWPPAL
                     TEPELLAHARELAAQNQRLLCFAGGGAQEHHIPTAVSALVQRGEFLTAYTPYQAEASQ
                     GTLQAMYEFQSSICALYGMAAANASLYDGATALAEAANAAARITGRRVILLPETVHPE
                     WRATLRTLFAARGEPQLVTLPCPAGRMEPEAVAARLDDSVAALVVPSPNYFGLLEDGA
                     ALAALAKAHGALVIAVADPISLGLLPAPGTWGADFAVGEGQGLGNPLNYGGPYLGLFA
                     CRQEYLRHVPGRLCGMTTDAAGRRGFVLTLQAREQHIRRERAASNICSNQALCALAAT
                     IHLALLGPAGLREVAELNVDRAHRLAAMIAAIPGFRLRFDGAFFNEFVVECPMSATRL
                     VKAMSKKGILAGVPLGKLYRGMDNSLLVCATEMRTTDDLERYAAALRAVDL"
     gene            complement(3715..4092)
                     /gene="gcvH"
                     /locus_tag="PKI64_10190"
     CDS             complement(3715..4092)
                     /gene="gcvH"
                     /locus_tag="PKI64_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOE01405.1"
                     /translation="MYPEANCRFTPSHEWVFVTGDVATVGISNHAQKEISDIVFIELP
                     KVGAKVTQGKPCAVIESVKAAADFFAPVSGEVSEVNAAIVDNPALVNQEPHGAGWFFK
                     IKMTQPAEADGLLSWADYQASCG"
BASE COUNT          864 a         1573 c         1310 g          707 t
ORIGIN      
        1 tggctgctcc ctgaactctg aaccctgacc tctgacctct ccgccgagcg ccccgcgagg
       61 cggggcggcg cgccagcgcg ggaaagacgc cggttggttt tggtgccgcc aaaattcaca
      121 agaaatcctc catccccaag tgccggaagc accagaacaa cccgcgccga cagctaccgc
      181 cctccgcccc cccccgcgca ctcaccgccg ctgaatccac tccggcgagc ggtaacgcac
      241 cgccaacgcc gtcgcagcat ctcggaccgc ttgtggcaca ggacagagct cccaccccgc
      301 caacccccac gccttcgcga tagcctccgc caccacggct tggtactcca cctcccgcgc
      361 gcgggcatcc gccaactgca acgagccttg gtatagcacc gtatcgccaa accgccggat
      421 tgcaccgccc agcaccttct cccccgccag cataatatcc atggtcaccg gctgcacaaa
      481 gcattgggaa gccccgcccg cgtgatgcgg cgcagcgctc ccgccgcgcg cgcacaactc
      541 caccggcagc cccatccccc gcagcccctc cccaatagcc gtgtgcagcc gccgatacac
      601 caccgccgca cgcatttcgc ccgcgctggg gaaaacgcac gaaaaggtga gatcgtgtaa
      661 atgtggcacc acgccgccgc cggtgggccg ccgggtgtag gccgtgccga ctgccggcgg
      721 cagcgtcgcc atgacttcgc ggacgcgctg cgcatagcca aaagtggccg cgggcccctg
      781 ccagcggtaa aaccgcaggc aaaagtgatc cggctgagcg cgggccaagg cttcgtcgca
      841 ggccatgtgg gcgaagacat ccgccggggg caattggagc aaatcggcca gcatgggtgc
      901 ctccctactg cggcggttgc gcgggccacg ccacgttggg attgcgcgcc gcgttcagtt
      961 catccaaccg gcgcaccggc agattgtgcg gcgcgctctt cacctgctcc ggcgcggtgc
     1021 gggcttcttc atgaatcgca cgcagagctg ccacaaacgc atccagcgtg gctggggttt
     1081 ccgtttcggt gggctcaatc atcagcgctt cgggcacatt cagcgggaaa taaatcgtcg
     1141 gtgcgtaaaa accgtgatcc agcaaacgct tggccacatc cagcgttttg accccgttca
     1201 atcggtccgg ttccgtccgc aagacgcatt catgcaggca cggttcatcc accgccgggg
     1261 ggaaaaggtc gcgcagtttc acgcgcaaat agttcgcgtt caaaatggcg ttctccgcca
     1321 cgcgccgcaa cgtggtggcg tcgtgccggc gcaggtaggc ataggccttc agcgccaccg
     1381 ccacgttgcc ggcaaaactt ttcacccggc cgatgctgcg cgcgcccttg gtttgcaacg
     1441 cgtagcgccc cttgcggcgc atcacgcgcg gcagcggcag gaatggttcc agcgtcgcgg
     1501 tcacccccac aggccccgcg cccggccccc cgccgccgtg tggtgtcgcg aaagttttgt
     1561 gcaagttcag gtgcaggata tccaccccga aatcggccgg tcgcgccagc cccagcagcg
     1621 cattgaaatt ggcgccatcc atgtagagca gcgcgccggg ttcatgcacc gccgcagcga
     1681 tctcgcggat ttgcgtttca aacaggccca gcgtattcgg caccgtcagc atcaccaacg
     1741 cagtttgcgg tcccagcgcc gcgcgcaacg cggccacatc cagccgccca ttcggctgcg
     1801 tggccaccgt tttcacggca aagccgccca acgccgccga agccggattg gtaccatgcg
     1861 cggtatccgg caccacgatt tccgtccgcc gtcggtcctt gcgcgcgcgg tgataggccg
     1921 cggcaatcaa aatgccggtc agctcgccat gcgcgccggc ggccggttgc agggtaaagg
     1981 ccgccagccc cgtcaactgg cagagtagtt gctccagccc atgcaatgcc gccagcgtgc
     2041 cttgcaccgc cgtttccggc gcggtggggt gcgccgccgc aaagccatcc tgccgcgcga
     2101 actcttccag cgagcgcggg ttatatttca ttgtgcagga gcccagcgga taaaaatgcg
     2161 tatccagaca gaagttcagt tgcgagagcc gggtgtagtg ccgcaccaca tccagctcgc
     2221 tcagttccgg caggcggggc gacgcgtcgc gcagcgccac gcccggcagc cacgccgccg
     2281 ccgaggccga tggcgcggga aaagtcagcg cgcgccgccc gggcacgctt ttttcaatgc
     2341 tcagtggctc gccacggttc ataggtccac cgcgcgtaat gccgccgcat agcgttccaa
     2401 atcgtcggtg gtgcgcattt ccgtcgcgca caccagcagg ctgttgtcca tcccgcggta
     2461 cagcttgccc agcggcactc ccgccagaat cccttttttg ctcatggcct tgaccagccg
     2521 cgtcgccgac atggggcact ccaccacgaa ctcgttgaaa aacgcgccgt caaaacgcag
     2581 gcggaaaccc ggaatagctg caatcatcgc agccaaccgg tgggcccggt ccacgttcag
     2641 ctccgccact tcgcgcagcc ccgccggccc cagcagcgcc agatggatgg tggccgccag
     2701 cgcgcacagc gcctgatttg agcagatgtt ggatgccgct cgttcccgcc ggatgtgctg
     2761 ctcgcgcgct tgcagggtca gcacaaatcc gcgccgcccc gcggcatcgg tggtcatgcc
     2821 gcaaagccgt ccggggacat gccgcaaata ctcttgccgg caggcaaaca agccgaggta
     2881 cggcccgcca taattcagcg gattgcccag cccctgcccc tcgcccacgg caaagtccgc
     2941 gccccacgtc cccggcgcgg gcaacaggcc gaggctgatc gggtccgcca ccgcaatcac
     3001 caaggcgcca tgcgctttcg ccagcgcagc caatgccgcg ccatcttcca gcaagccaaa
     3061 atagttcggc gatggcacca ccagcgccgc cacggaatca tccagccgcg cggccacggc
     3121 ctccggctcc atgcgcccgg cggggcaagg tagcgtgacc aattgcggtt caccgcgcgc
     3181 ggcaaacaac gtgcggagcg tcgcccgcca ctccggatgc accgtctctg gcagcaggat
     3241 tacccgtcgt ccagtgatgc gtgctgccgc gttggcagcc tcggccagcg ctgttgcacc
     3301 gtcgtagagc gacgcattgg cagccgccat gccgtacagc gcgcaaatgg agctttggaa
     3361 ctcatacatc gcctgcaacg tgccttggct ggcttcggct tggtacggcg tgtaggccgt
     3421 caaaaattcc ccgcgctgga ccaacgccga caccgccgtc ggaatatggt gctcctgcgc
     3481 gccgcccccc gcaaagcaga gcaaccgctg gttttgcgcc gccagctcgc gcgcgtgcgc
     3541 cagcagttcc ggctcagtca acgccggcgg ccaagccgtc acgcttgctt gcaattcggc
     3601 gggaatctgc gccagcaggt ccgccagcga ccccacgcca atgtcgcgca acatcgcctg
     3661 ttgttcggcg gctgtgtggg gggagtacat tcgaaaaaac ggaatgccgc cgtctcagcc
     3721 gcaagaggcc tgatagtccg cccacgagag caggccatcc gcttcggcgg gttgcgtcat
     3781 cttgatcttg aaaaaccagc ccgcgccatg cggctcctga ttcaccagcg cgggattgtc
     3841 cacaatggcc gcattcactt cgctgacctc gccggagacc ggcgcaaaaa agtccgccgc
     3901 cgccttcacc gactcgatca ccgcgcacgg cttgccttga gtcaccttcg cccccacctt
     3961 cggcagctca atgaaaacaa tatcgctgat ctctttttgt gcgtggttgc taatgcccac
     4021 agtggcgaca tctcccgtca caaacaccca ttcatgggac ggggtaaagc ggcaattggc
     4081 ttctggatac atggcagttc tccaatcttg atcgcccccc agtgtgccgc atccacccgc
     4141 ccaaatccag aacgaaggcg ttgcgctgcg cgcaaggtgt cagggttcag agttcagggt
     4201 tcaggggaac agccgggaac atttaacgca aaagacgcaa gaggcgcaaa agggcgcaaa
     4261 agaaagacta gagactggag acggaagcca gaacagccga gcaggaaagc aggaaaccca
     4321 ggaaaacagg aaaagaacga ttctcgccga gacgcaaaga gcgcgaagaa gagactacag
     4381 accagagact atagactaga gacggtggag ggttgcaggt ttcaagtttc aggcaatagc
     4441 cgaacagccc gccg
//
LOCUS       DAOPNP010000148         4450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_21282_length_4450_cov_7.063481, whole genome shotgun sequence.
ACCESSION   DAOPNP010000148 DAOPNP010000000
VERSION     DAOPNP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21282_length_4450_cov_7.063481"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKI64_10195"
     CDS             <1..366
                     /locus_tag="PKI64_10195"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053706.1"
                     /note="catalyzes the formation of oxaloacetate from
                     L-aspartate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOE01406.1"
                     /translation="AWKTGGAVPSDEAVVVSHNWNEVRTVMWDYVGIVRTNRRLARAL
                     RRIGTLRREIRAYYRDYLVTSDLLELRNIAAVAELIIRSAMMRRESRGLHYTLDYPYL
                     ANSMCHDTIIHDLPGGGAV"
     gene            371..1189
                     /locus_tag="PKI64_10200"
     CDS             371..1189
                     /locus_tag="PKI64_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOE01407.1"
                     /translation="MPWFLQIPYGLLVGVAGLLPGVSGSTLLVLGGLYRPLTGALAHP
                     LRDFRRLLLPLAPFLLAAGLGLLLGSRLLSAFFARHETAALYLFMGLMFGALPHLWQH
                     AHRAGGGRRGWWLGGLMFLLCVGGALLDNHVAPRPDTIAARWSIWLLAGAGIGLGSVI
                     PGASVAFILIYLGLYQPLLDGVAALDLNVLLPLGGGALAAILGFARLADWLFRHAEGL
                     TTRAIFGLVAGSIPLLYPSWPGGTAGLRALLLFAAGAVLSTLLNKYAPDAPARG"
     gene            1410..2993
                     /locus_tag="PKI64_10205"
     CDS             1410..2993
                     /locus_tag="PKI64_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOE01408.1"
                     /translation="MTPTQEIARRRTLAIISHPDAGKTTLTEKLLLYGGAVQLAGSVT
                     ARKNQRATTSDWMELEKQRGISVSSTVLQFDYNNYVVNLLDTPGHHDFSEDTYRVLMA
                     VDAVIMVLDAAKGIESQTLKLFEICRLRGIPIFTFINKLDRPSRAPLGLLDEIENTLQ
                     LQACAMNWPLGDGVDFRGVYDRQGKLAHFFERTVGGASRAPEVLRSLQDLATLSQMPA
                     DAVARLTEEIAMLDAAGAVFDEAAVRAGDMTPVFFGSALNNFGVQLLLDFFLAHAPPP
                     GPRKAGALVVPPQHDQFSGFIFKIQSNMDPQHRDQIAFLRICSGVFQRDMKATHPRTG
                     KVIRLSNSRKLFARDRETVDEAYPGDVIGLVGHPEFGIGDTLTADPAIVYDPLPQFAA
                     ECFAWLHHASPAQFKRFRAGLDHLLQEGAVQTFTLPDSGSRAPLLGAVGPLQFEVLQY
                     RLENEYGAVTRREAAPWTILRWVDPAGEPVSPAMLPSSCRLAFDTANRPVALFSADWE
                     LKFFQEKNPRVILQRLPPA"
     gene            3058..3768
                     /locus_tag="PKI64_10210"
     CDS             3058..3768
                     /locus_tag="PKI64_10210"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOE01409.1"
                     /translation="MAEPVEKLVNEVKYVGTERHTETVSALLAAACQPDGEYPVGAIN
                     SIYFDTPNRQFHDEKLNGDNLKLKVRLRWYGRPGPLTDEDVPVFIEAKHRLGSARRKT
                     RIGVRAPERWLATVPLDDPGLVDFLYRHAAELGEAIPHILSPSVCISYSRQRYICPVT
                     GSRVALDWQIRAGRVNPARFPTVGPVHLDCIVVEFKNQTKELPAWSYPLFQMGLRLRS
                     FSKYGECMNQVLQGGAPL"
     gene            3765..>4450
                     /locus_tag="PKI64_10215"
     CDS             3765..>4450
                     /locus_tag="PKI64_10215"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOE01410.1"
                     /translation="MNNVNTFGVLESLRQIREQLDPGLFLLALLASLAAAYAAAGFYR
                     FFYERRGTGSQVHRAFPLLGIAITTLFIGVQLSIPLSLGLLGALSFIRFRTPIKEPEE
                     VGFIMLVIAASISAATFNFRFLVIMYLVAFGSLLLTRGTRFWRLWRRDGILVLTIPAG
                     DATTHMTAVEEALGRHLQRYRLESSSFREDQASFQYVFSGLKTDVSRLQAELRGQAPL
                     QTLNVFLDRPG"
BASE COUNT          773 a         1394 c         1320 g          963 t
ORIGIN      
        1 gcgtggaaaa ccggcggggc cgtgccctcc gatgaagccg tcgtggtttc gcacaactgg
       61 aatgaagtgc gcaccgtcat gtgggactat gtgggcattg tgcgcaccaa ccgccgcctg
      121 gcccgcgccc tgcggcgtat tggcaccctg cgccgagaaa tccgcgcgta ttaccgcgat
      181 tatctcgtga cctccgacct gctggaactg cgcaacattg ccgcggtagc ggaactgatt
      241 atccgctcgg ccatgatgcg ccgcgaaagc cgtggcctgc actacacgct ggactacccg
      301 tatctggcca acagcatgtg ccacgacacc attatccacg atctgcccgg cgggggcgcg
      361 gtttgatccc gtgccttggt ttctgcaaat cccatatggc ctgctcgtgg gtgtggccgg
      421 cctgctgccc ggagtcagcg gcagcacact actcgtgctg ggcggactct accggccgtt
      481 gaccggcgct cttgcccacc cgctgcggga tttccgccgc ttgctgctgc cgttggcccc
      541 gtttctcctg gcagcgggtc tcggattgct gcttggcagc cggcttttgt ccgctttttt
      601 tgcgcgccat gaaaccgccg cgttgtacct ctttatgggc ctcatgttcg gcgcgctgcc
      661 gcacctgtgg caacacgccc accgcgcggg cgggggccga cgcgggtggt ggcttggtgg
      721 actcatgttt ctgttgtgcg tcggcggcgc tttgctggat aaccatgttg cccctcgccc
      781 cgacaccatt gccgcacgct ggagcatctg gctgttggct ggggcgggca ttggtttggg
      841 cagcgtgatt cccggcgcca gcgtggcttt tattttaatt tatttgggcc tttaccagcc
      901 cttgctcgat ggcgtggccg cgttggattt gaacgtcttg ctgccccttg gtggcggcgc
      961 gctggccgcc atccttggct ttgcccggct ggccgactgg cttttccgcc acgcagaagg
     1021 ccttaccacc cgcgccattt ttgggctggt ggccggctcc atcccgctgc tctaccccag
     1081 ttggcccggc ggcaccgccg gtctacgtgc cctgcttctt tttgccgccg gcgcggtcct
     1141 ttctaccctg ctcaacaaat acgccccgga cgctcccgca cgagggtgac gacctgcgac
     1201 catccagccc cacaatccaa cccgcaatcg ggcttcgggc cgggacactc caaaatgaaa
     1261 gcggccattt gcagtgcaac gagtgacaaa gagtttttca gggtgcgtgg aactccggcg
     1321 atgaaacggg aaaattcact cgcctttttt ccgaggtgtg gaagactgcg cggattcgat
     1381 gattttgttg tagaagacgc acacgaccca tgaccccaac ccaggaaatt gcccgccgcc
     1441 ggacactggc gattatttcg cacccagatg ccggcaagac gacgcttacg gaaaagttgc
     1501 tgctgtacgg cggggcggtg caattggccg ggtccgtcac ggcccgcaaa aaccaacgtg
     1561 cgaccacatc cgactggatg gaactggaaa agcaacgcgg gatctcggtc agttccaccg
     1621 tcctgcaatt cgattacaac aactatgtgg tcaacttgct cgacaccccc ggccaccacg
     1681 atttttccga ggacacctac cgcgtgctta tggccgtgga cgcggtgatc atggttcttg
     1741 atgccgccaa gggcatcgag agccagacgc tcaagttgtt cgaaatctgc cggctgcgcg
     1801 gcatccccat tttcacgttc atcaacaagc tcgatcgccc atcccgcgcg ccgctcggcc
     1861 tgctggacga aattgaaaac accctgcaac tgcaagcatg cgcaatgaat tggccgctgg
     1921 gtgacggcgt agatttccgc ggggtctatg accgccaagg caagttggcg cacttttttg
     1981 agcggacggt tggcggcgcc tcgcgggcgc cggaagtgtt gcgttcgctg caggatttgg
     2041 caacgctcag tcaaatgccg gccgatgccg tcgcgcggct gaccgaagaa attgccatgc
     2101 tggacgccgc cggcgcggtg tttgatgaag ccgcggtgcg cgccggcgac atgactccgg
     2161 tgttttttgg cagcgcgctc aataacttcg gcgtgcaact gctgctggat ttctttctgg
     2221 cccacgcccc cccacccggc ccacgcaagg ccggagcgct ggtggtgccg ccacagcatg
     2281 accaattctc cggcttcatt ttcaaaatcc agtccaacat ggatccccag caccgcgacc
     2341 aaatcgcatt tttgcggatt tgttccggcg tgttccaacg tgacatgaag gccacccacc
     2401 cccgcactgg caaggtgatc cggctctcca actcgcgcaa gctgtttgcc cgcgaccgcg
     2461 aaaccgtgga tgaagcgtat cccggcgatg tcatcggcct ggtggggcat ccggaattcg
     2521 gcatcggcga cacgctgacg gcagacccgg ccatcgtgta tgacccgttg ccgcagtttg
     2581 ccgcggaatg tttcgcgtgg ctgcaccatg ccagccccgc gcaattcaag cgattccgcg
     2641 cggggctgga ccatctgttg caggaaggcg cggtgcagac gttcactttg cccgactccg
     2701 gttcccgcgc gccgttgctg ggcgcggtgg gcccgctcca gtttgaagtc ctgcaatacc
     2761 ggctggaaaa tgaatatggc gcggtcaccc gtcgcgaagc tgccccgtgg accattctac
     2821 gctgggtgga ccccgccggg gaacccgtca gcccagccat gctgcccagc agttgccggc
     2881 tggccttcga caccgccaac cgcccggtgg cactgttttc tgcggattgg gaactgaagt
     2941 tttttcagga aaaaaatccg cgcgtcattc tgcaacgcct gccgccggct tgaaatcggt
     3001 tgatttcagc cctctttatg ccaccatggg gccatggtgc tgcagtggtg tcaatctttg
     3061 gccgaaccgg ttgaaaaatt ggtcaacgaa gtcaaatacg tgggcacgga acggcacacg
     3121 gaaacggtca gtgcgctgtt ggccgcggct tgccagcccg acggcgaata ccccgttggc
     3181 gcgatcaaca gcatttattt cgacactccg aaccgccaat tccacgacga aaaacttaac
     3241 ggcgacaatc tgaaattgaa agttcgcctg cgatggtatg gccgccccgg tcccctcacg
     3301 gatgaggatg taccggtgtt catcgaagcc aagcatcgcc tcggttccgc ccgccgcaag
     3361 acgcgtatcg gggtacgtgc gcccgagcgc tggctggcca ctgtgccgct ggatgatccc
     3421 gggctggtgg atttcctcta tcgccacgcc gcagaactgg gcgaagcgat cccgcacatc
     3481 ctgagcccct cggtttgcat ctcgtattcc cggcaacgct atatctgccc ggtcacggga
     3541 agccgagtgg cgctggattg gcaaattcgg gccggccgcg tcaatcccgc gcgctttccc
     3601 accgtgggcc ccgttcattt ggactgcatc gtggttgagt tcaagaatca gaccaaggaa
     3661 ctgccggcct ggagctatcc gcttttccag atgggcttgc ggttgcgtag tttctcaaaa
     3721 tatggtgaat gtatgaacca agtcctgcag ggaggcgcgc cgttatgaat aatgtaaata
     3781 catttggagt gttggagagt ttgcggcaaa tccgggaaca actggatcct ggattgtttt
     3841 tgctggcgct gctggcttcg ctggcggcag cctacgcggc ggccggcttc taccgctttt
     3901 tctatgaacg gcgcggcaca ggcagccaag ttcaccgcgc tttcccgctc ttgggcatag
     3961 ccatcaccac gctgttcatt ggcgtgcagc tctccatccc cctctcgctg ggcttgctcg
     4021 gcgcgttgtc gttcatccgc ttccgtacac ccatcaagga gccagaggaa gtcggcttca
     4081 tcatgctggt gattgcggca tccatttcgg cggccacgtt caacttccgc tttctggtga
     4141 tcatgtattt ggtggccttc ggcagcctgt tgctgacgcg cgggacacgc ttttggcggc
     4201 tttggcggcg tgacggtatt ctggtgctga caatacccgc aggcgatgcc accacgcaca
     4261 tgaccgcggt ggaagaagcg ctgggccgcc atttgcagcg ctaccggctg gaaagttcat
     4321 ctttccggga ggatcaggcc agctttcagt acgttttttc cggactgaaa accgacgtgt
     4381 cccgcctgca agcggaattg cgcgggcagg cgccgttgca aacgctgaac gtctttttgg
     4441 atcggcccgg
//
LOCUS       DAOPNP010000149         4202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_22913_length_4202_cov_9.142754, whole genome shotgun sequence.
ACCESSION   DAOPNP010000149 DAOPNP010000000
VERSION     DAOPNP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22913_length_4202_cov_9.142754"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..600
                     /locus_tag="PKI64_10220"
     CDS             313..600
                     /locus_tag="PKI64_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE01411.1"
                     /translation="MAKAMTKSQITAAVAEKAGISKKQAGAALEALSALAYKNAKNGF
                     TVPGLGKLVLVNRKARQGRNPQTGEIIQIKAKKVVKFRVAKAAKDAILGAK"
     gene            complement(662..1993)
                     /locus_tag="PKI64_10225"
     CDS             complement(662..1993)
                     /locus_tag="PKI64_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOE01412.1"
                     /translation="MKTLHLVRWAVGICLGALLVGGATAQNPPVTLRVGLYQNPPEIF
                     TDETGQPAGIFVDILAAIAEAEHWTLDYVPGSWAENLDRVATGEIDLMPGVAITAERA
                     ERYAFHAEPVLSDWFQIYAPPDATIRSLLDLDGRRVATVSHSLQEEAFTTLANGFGLH
                     TTLVPAKDFAAAFDMVLNGQADAVVASRFAGSYLQRTKRLADTAVIFNPSRSFFVAPP
                     TVNPLILRIIDQHLVRMKQDPDSVYHHALRHWATEDVHTRIPRWLPATGAALAVLLLL
                     VLVWNAALNRQVARRTAQLELQNEESRLLASRVKAGEARMRFHAQLLDSVRESVVASD
                     LEGRITYWSRGAERLYGYSAVEVMGKPYRDFAGPIQPPPEEDFRRELIARGCWQGEHQ
                     QKNRRGETFWTSTFVSLVRDEDGRHAGYIGLDLPLTEKQAEHLQNQSRCRD"
     gene            complement(2210..3112)
                     /locus_tag="PKI64_10230"
     CDS             complement(2210..3112)
                     /locus_tag="PKI64_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01413.1"
                     /translation="MADITFDCPQCGNNLVVDESGAGLSVPCPECSQLITIPSPTIAA
                     RPTPPQGDIVFNCPHCKQSIEAPLAIFGQLIDCPACNNPVEVRRSSSLPMPPPRRPTT
                     PPPHIAPKVPRPVPGPHPHHISSIKVISGRTIAALIFGAIVLVAWTRNYFFVQKPVAD
                     TIRADSRNYGYKLTARLQYYILPSTLILDLVKVKSVAPVDLFRGLFQSAEVFSDARRR
                     FDKVILARNGKPIFEISGSDFWTLGSEYGSGQNPMYLIRTLPEKLYRPNGRPAYSTWE
                     GGFLGVVSRQMEDATDAAHTWADY"
BASE COUNT          954 a         1165 c         1149 g          934 t
ORIGIN      
        1 tcccaagagc ggcaaggcgc gcaaaaatta cgcggatgat ctggaagcga tgctcacgca
       61 aaaaatcacg ccgcgcctcg tgcccaccac cgcgtgcagt ttgcgcatcc gcaccgcggc
      121 ggatctcgcg ccggccatcg cgttgatgaa atgaccgcgc aggaatttgc cgcaccccct
      181 tgcgccgcga tgaaaatatt ttggaaaaaa ggttgcattt cttccgtatt tggaggataa
      241 acgccctcca gattgggcat ccatttaaaa acccatctta aaaaaagaaa cagaaaacaa
      301 ggagtactgg ttatggcaaa ggcgatgaca aagagtcaga ttacggctgc ggtggctgaa
      361 aaggctggca tctcgaagaa gcaggctggc gcggcgctgg aagccctttc ggctttggcg
      421 tacaagaatg ccaagaatgg tttcaccgtt cctggtttgg gcaagctggt gctggtgaat
      481 cgcaaggcgc gccagggccg caacccgcag accggcgaga tcatccagat caaggccaag
      541 aaggttgtga agttccgcgt ggccaaggct gcgaaggacg ccatcctcgg cgccaagtaa
      601 ttcatccctc agatgaaacg aaagccgcgg cagatgccgc ggcttttttg ttggctgcaa
      661 atcaatcgcg gcagcgcgac tggttttgca gatgctccgc ctgcttctcg gtcagcggca
      721 aatccaggcc gatgtagccc gcatggcggc catcttcatc gcgcaccagc gagacaaatg
      781 tggaggtcca gaaagtttcg ccgcgccggt tcttctgctg gtgttcgccc tgccagcaac
      841 cccgcgcaat caactcgcgc cggaaatctt cttcgggcgg cggctggatc gggccggcaa
      901 aatcgcggta gggtttgccc atgacctcca cggcggaata gccatagagc cgttctgcgc
      961 cgcggctcca gtaggtgatg cggccttcca gatcggaagc caccacggac tcgcgcacgc
     1021 tgtcgagcag ttgcgcgtgg aaccgcatcc gcgcctcgcc ggccttgacc cggctggcca
     1081 gcaggcggct ttcttcattc tgcagttcca attgcgcggt gcgccgcgcc acctgccggt
     1141 tcaacgcggc attccaaacc agcaccagaa gcaacagcac agccagcgcg gcgccagtcg
     1201 ccggcaacca gcgcggaatg cgcgtgtgca cgtcttctgt ggcccaatgc cgcaaggcat
     1261 gatgataaac ggaatccgga tcctgcttca tccgcaccag atgctggtca atgatgcgca
     1321 ggatgagcgg attcaccgtg ggcggcgcca cgaaaaacga gcggctggga ttgaagatga
     1381 ccgccgtgtc cgcaaggcgc ttcgtccgct ggagataact gccggcaaaa cggctggcca
     1441 ccacggcatc cgcctgcccg ttgaggacca tatcaaacgc tgccgcaaaa tccttggcgg
     1501 gaaccagcgt ggtgtgcagg ccgaatccgt tggccagcgt cgtgaaggct tcttcctgca
     1561 acgagtggct gaccgtggcc acgcgccgcc catccaaatc cagtagtgaa cggatggttg
     1621 catccggcgg cgcgtaaatt tgaaaccagt cggacagcac cggctcggca tgaaacgcgt
     1681 agcgctctgc gcgttcggcg gtgatggcta caccgggcat caaatcaatt tcacccgtgg
     1741 ccacgcggtc cagattttcg gcccagctcc cgggcacata atcgagagtc cagtgctcgg
     1801 cttcggcaat ggccgccaaa atgtccacga agatgccggc cggttggccc gtctcatccg
     1861 tgaaaatttc cggcggattc tggtacagcc cgacgcgcag cgtgacgggc gggttttgcg
     1921 cggttgctcc acccaccagc agcgccccaa ggcagatccc aacagcccaa cgaacaagat
     1981 gcagtgtctt cataggttcg ccaaccagcc taagccatca cccgaacatt gacaagcccc
     2041 gggcatcacc caagcgccaa acccacccgg atcgaccacc tcgcggcacg ctcccgctct
     2101 actccaatca tcttttcaca aattcactta ccgcgcaggt ggcgttctgc gcaattcgcg
     2161 tgttatctcc atgaaatcaa aacgaagaaa tcggagttat aaaacgacat taataatcag
     2221 cccaggtatg agccgcatct gtcgcatcct ccatttgcct gctcacaacc cctaaaaatc
     2281 caccttccca agtgctatag gcggggcgac cattgggacg gtaaagtttt tccggcaatg
     2341 ttcttatgag atacatcgga ttctgtcccg acccgtactc ggaaccaagt gtccagaaat
     2401 cgcttccgct gatttcaaat attggttttc cgtttcgcgc aagaatgacc ttgtcaaatc
     2461 tcctccgagc gtcagaaaat acctcagcgg attggaaaag cccgcgaaac aagtcaacgg
     2521 gagcaacaga cttcacctta actaaatcaa gaataagagt tgaaggcagt atgtaatact
     2581 gtagccgcgc agtaagctta tacccataat tccttgaatc tgcacgtatg gtgtcagcca
     2641 ctggtttctg gacaaaaaag taattacgcg tccaagcaac gagaacaatt gccccaaaaa
     2701 ttaaagccgc aatagtgcgt ccggagataa cctttatgct gctgatgtga tgaggatgcg
     2761 gcccggggac tgggcgcggg actttaggag ctatatgggg aggaggtgtg gtcggtcgtc
     2821 tcggcggagg cattggtaat gacgaggacc gtctcacctc aacagggtta ttacacgcag
     2881 ggcagtctat cagttggccg aatatagcaa gtggcgcctc aattgactgt ttgcagtggg
     2941 gacaattaaa aacgatatcg ccttgaggtg gagttggcct tgccgctatt gtgggggacg
     3001 gaatcgttat gagttgagaa cattcaggac atggcaccga caagccggca ccactctcat
     3061 ctactacgag gttattacca cactgcgggc aatcaaaagt tatatctgcc atgtgtttcc
     3121 ttatcttgaa cgccaatggt cggtgcccgg caccttgtgc caggttcctg gccccctttg
     3181 ctcataatct tagtcactat caataacgtc taccctagac acaacacagc ttaatcccgt
     3241 ctgtgatttg catatcgcaa acctattggt ttgctttgca tagcacgtca cttcttttgc
     3301 gccgccgaat cctccagtat atttgacata cattttggtg tcgcggttga ttggcacctc
     3361 gaacacttct ccgggcgaaa gcctcgcaag tacacttttg ttggcgtcca taatactgta
     3421 ccaggcaagc aatccaatga attcacttga tgccttgaat atggctttcc cctgtttgtt
     3481 cagccattcg ccacagtgcc tacatttctg aaccgtcgga agtactgggc ctccgcaata
     3541 gggacaaggt tgtgtttgtt gttgcgggct tggtggcggc gtcggcggca gtggcgctgg
     3601 cgatggaggt ataatcggtt gccccgatag gggcttgagc atggaggacc gtctcacctc
     3661 aacagggtta ttacacgcag ggcagtctat aagttggccg aacatagcaa gtggcgcctc
     3721 aatcaactgt ttgcagtgct ggcaattaaa tacgatatca ccttggggtg gagtgggtct
     3781 cggcgctgtt gcgggggaag gaatcgttat gatctgggaa cattcagggc atggcgccga
     3841 caagccggca ccactctcat ctactacgag gttattacca cattgcgggc aatcaaaagt
     3901 tatatctgcc atgctgcctc catatcttga atggcttgtt caactccttc attttgtctg
     3961 gccgaggcgt ttgcgtccag aaaattggtg tttttgcgtt ggaaatggca ggttttcatg
     4021 ttgttggggc tattttgggg tgtttttggt gatttttggg cgcgaaaccg gcaccttttc
     4081 tcattttttg gcgtagcgtc acttctccgc gagggcgttg gggttttgga gatcactcct
     4141 catttgttga acgttagcga tgaggctgag gcggcttgcc gccgatagcc tcaagcgcct
     4201 tg
//
LOCUS       DAOPNP010000150         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_24578_length_3978_cov_12.003824, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000150 DAOPNP010000000
VERSION     DAOPNP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24578_length_3978_cov_12.003824"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1381
                     /gene="eno"
                     /locus_tag="PKI64_10235"
     CDS             113..1381
                     /gene="eno"
                     /locus_tag="PKI64_10235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOE01414.1"
                     /translation="MFQIAAIRAREILDSRGNPTLEADVQLTCGALGRAAVPSGASTG
                     ANEALELRDGDAKRYLGKGVQKAVKNVNTLIAKALIGMDARNQKAVDDALLKLDGTET
                     KAKLGANAMLGVSMAVARAAAAALEIPLYRHLGGLNAHVLPVPMMNVINGGAHSDAPI
                     DVQEFMIVPRGAKTFAEGLRMGTEVFHALKKILKSRKLSTAVGDEGGFAPALGSNTEA
                     LEVLVAAISAAGYKPGKDVFIALDVASSEFFDAKTRKYTLHKSDKSVKSAADMVAMYE
                     GWCKKFPIVSIEDGCAESDWDGWKLLTDKLGAKGVQLVGDDLFVTNVKFLQKGIDLGV
                     ANSILIKVNQIGTVSETLAAIDLAKRHGYTNVISHRSGETEDSFIADLAVAVNAGQIK
                     TGSLSRTDRICKYNQLLRIEEELGRAAVYG"
     gene            complement(2079..2936)
                     /locus_tag="PKI64_10240"
     CDS             complement(2079..2936)
                     /locus_tag="PKI64_10240"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE01415.1"
                     /translation="MPNSYATPAAFRQALEARLIDLAYRRGMDIQRLRKQVAFDRLLC
                     RLFQLPGNPLVLKGGYAMELCLAENRTTRDIDLVARDTLPGPGRVQARILRLLQAGAT
                     LDLQDFFAYSIGQPSRNLEGAPYGGARYPVEVRMAGRIFARFHLDVASGDPVLEPLQS
                     VHGADWLAFAGVGSAPFSVISKEQQFAEKLHAYTLPRAGRVNTRVRDLVDMLLLVQSG
                     LNGRRTQNDVRVVFKHRGTHPAPAALPAPPDNWNAPFAALAMECGLAIAIGEAYEEVS
                     SFCINKRIL"
     gene            complement(2936..3526)
                     /locus_tag="PKI64_10245"
     CDS             complement(2936..3526)
                     /locus_tag="PKI64_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOE01416.1"
                     /translation="MKNKADRLYRVAERQQGYFTAGQAVACGYPTSSHVYHRKQGAWQ
                     REGRGIYRLSRYPTTDDAQYVRWTLWSRNKQGVPQGVLSHQTALTLFDLSDLMPARIH
                     LTVPLDFRRSATPPKVLVLHRGVLTKNDIEERQGYRVTRPLRTIADLLQDPAVAPNQL
                     WMALTQGLKRGLITRTEMAAHPQAKALKKLLHKGWL"
BASE COUNT          864 a         1105 c         1240 g          769 t
ORIGIN      
        1 gaaggcgggg gtgcaaaaaa gttgccaatc ggcggggggc gtgtagagta ggctggcaaa
       61 aacgcccgtt tggcgggcag cttgaacccg tagaaaccga aggagagatc ccatgttcca
      121 gattgcagcg atccgcgccc gtgaaattct tgattcgcgc gggaatccca cccttgaagc
      181 cgatgtgcag ttgacgtgcg gcgcgctggg ccgcgcagca gtgccgtccg gcgcctccac
      241 cggcgcaaat gaagcgctgg agctgcgcga cggcgatgcc aagcgctatc tgggcaaggg
      301 cgtccagaag gcggttaaaa atgtgaatac gctgattgcg aaggcgctga tcgggatgga
      361 tgcgcgcaac cagaaggcgg tggatgacgc gctgctgaag ctggacggca ccgaaaccaa
      421 ggccaagctg ggcgccaacg ccatgctggg cgtttccatg gccgtggcgc gcgcggcggc
      481 ggcggcactg gaaatcccgc tctaccggca cctcggcggg ctcaatgccc acgtgctgcc
      541 cgtgccgatg atgaacgtca tcaatggcgg cgcgcattcc gatgcgccga ttgacgtgca
      601 ggagttcatg attgtgccgc gcggcgccaa gacattcgcc gagggcctgc ggatgggcac
      661 cgaggtgttc cacgcgctga agaagattct caagagccgc aagctgtcca ccgccgtggg
      721 cgatgaaggc ggttttgcgc ctgcgctggg cagtaatacg gaagcgctgg aagtgctcgt
      781 ggccgccatc agcgcggcgg gctacaagcc gggcaaggac gtgttcattg ccctcgatgt
      841 ggcttccagc gagttctttg acgccaagac ccgcaagtac acgctgcaca agagcgacaa
      901 gtccgtgaag agcgccgccg acatggtggc aatgtatgag ggctggtgca aaaagttccc
      961 cattgtgtcc atcgaagacg gttgcgccga aagcgattgg gatggctgga agctgctgac
     1021 cgacaagctc ggcgccaagg gtgtgcaact cgttggcgac gacctgttcg tcaccaacgt
     1081 caagttcctg cagaagggca ttgatctggg cgtggccaac tccatcctga tcaaggtcaa
     1141 ccagattggc acggtgtctg agacgctggc ggcgattgat ctggccaagc gccacggcta
     1201 cacgaacgtg atcagccacc gctcgggcga gacggaagac agcttcattg ccgacctcgc
     1261 ggtggcggtg aacgcgggcc agatcaagac cggctcgctc tctcgcaccg accgcatctg
     1321 caagtataac cagttgctgc gaatcgaaga agaattgggt cgcgcggccg tgtacggttg
     1381 aggcgcctag gagaggtcag agttctgagg tcagaggtca gtagaaaacc agcagccgaa
     1441 cagccccggc ctcagcgagg ccggctacag ggttcttggt tcgtggtgct tggttgagca
     1501 gccggaacag cccgccgggg attacaaccc cggcctacaa aaggcgtggc tgtttctgta
     1561 gccgcgggcg gtgaacgcgg gaattttttc ggacaccggc ctcaccgagg ccggctacag
     1621 gaagaccagt aaccaacagc ccagatggca aaattcctga accctgaacc ctaaaatctg
     1681 acccctccct tgtccgctgt gcgcagcacg tgcatgcgga ggatttcttg cggattttgg
     1741 cggggctgtt gcgccaaaat aaccggtaga ttttccgatg atggcgcgtt gcggacgacc
     1801 cgagctggac tcgtgtccgc gagcggtcgg tcgcggcgcg ccagcgcgga aaagatgccg
     1861 gttggttttg gcgccgccaa aatccgcaag aaatcctcca ccccaaacgc cggaagacgg
     1921 tggacggttg caggttaaag gttgcaagtt acaggagaac ggccagaaca gccaaacagc
     1981 cccggcctca gcgaggccgg ctacaggaag gccaacagcc gaacagcccg ccggggatac
     2041 aactgcggcc tacattcacg cttgaatttg gaacgggttc agagaatccg tttgttgatg
     2101 caaaaggacg aaacctcttc gtatgcctcg ccaatagcaa tagccaaacc gcactccatg
     2161 gccaaagcgg caaacggcgc gttccaatta tcgggcgggg ccggtaaggc ggctggcgcg
     2221 ggatgtgtcc cccgatgttt gaatactacc cgaacgtcgt tttgtgtccg ccggccattc
     2281 agaccgcttt gcacgagcaa aagcatatcc accaaatctc tcacccgcgt attgacccgg
     2341 ccggcacgag gcaatgtgta ggcatgtagt ttttcagcaa attgctgctc tttggaaata
     2401 actgagaaag gcgcagaacc aactccagca aaagcgagcc aatcagcacc atgaaccgat
     2461 tgcaaaggct ccaaaacagg gtcgccggaa gccacatcca aatgaaacct ggcaaaaatt
     2521 cgcccggcca tgcgaacctc gaccggataa cgtgcgccgc cgtagggcgc gccctctaaa
     2581 ttgcgtgaag gctggccgat ggaataagca aaaaagtcct gaagatcgag ggtggcgccc
     2641 gcctgcaaca aacgaagaat gcgcgcttgc acccgtccgg ggccgggcag tgtatcgcgt
     2701 gcaaccagat caatgtcgcg agtggtacga ttttcggcca agcacaattc catcgcatag
     2761 ccgcctttca aaaccagcgg gttccccggc aattgaaaca gacggcataa tagccggtcg
     2821 aaggccacct gtttgcgaag gcgctggatg tccatgcctc tacggtacgc caaatcaatc
     2881 agccgggctt ccaacgcttg cctgaatgcc gctggtgttg catatgagtt tggcatcaca
     2941 accatccttt atgcaggagt tttttcaggg ccttggcttg ggggtgtgcg gccatttcgg
     3001 tgcgggtgat caggccacgc ttgaggcctt gcgtcagggc catccatagt tggttgggcg
     3061 caacggccgg atcttgcagc aagtcggcga tagtccggag tggacgggtg acacgatacc
     3121 cctggcgttc ctcgatatcg ttcttcgtca gtacgccgcg gtgcagaaca aggactttag
     3181 gcggggtggc actccgccgg aaatccaatg gcacagtcag gtgaattcga gccggcatga
     3241 ggtccgacag atcaaacaac gtcagtgccg tctggtgtga gagaacccct tgaggcactc
     3301 cctgcttgtt gcgcgaccaa agtgtccagc ggacatattg ggcatcatcc gtcgtgggat
     3361 agcgcgacag acgatagatt ccccggcctt ctcgttgcca ggccccttgc ttacggtgat
     3421 atacatggct agaggttgga tagccgcaag ccacggcctg tcctgccgta aaatatccct
     3481 gttggcgttc cgccacgcga taaagtcggt ccgccttgtt tttcatcgcc aacctccatt
     3541 tgcatataag ctatattaat tatagtgttt gtgcaatccc tgtttagatt gtattttttg
     3601 caagacctca cttgggcgcg tttgcggcgc tggcggcacg ggcgttgggg ttcttattct
     3661 ggacggcttg gcggaattta ttgctctact gtagccgcgg gcgtgaacgc gggaggagag
     3721 gtcagagggc tgaggtcaga gggcaggaga aaaccagcag ccaacagccc cggacggttg
     3781 caggttaaag gttgcaggtt tcaggggaac agccgaacag ccccggcctc ggcgaggccg
     3841 gctacagggt tcttggttcg tggtgcttgg tttttggtgc ttggttgagc agccgaacag
     3901 cccgccgggg attacaaccc cggccttcta tgggggccgt caagtcatgg cgatggattt
     3961 attgtcttac tgtccttt
//
LOCUS       DAOPNP010000151         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_24687_length_3966_cov_7.023779, whole genome shotgun sequence.
ACCESSION   DAOPNP010000151 DAOPNP010000000
VERSION     DAOPNP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24687_length_3966_cov_7.023779"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..396
                     /locus_tag="PKI64_10250"
     CDS             91..396
                     /locus_tag="PKI64_10250"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOE01417.1"
                     /translation="MKLSLAHQQFQQRPGVVLRKVAGEVMLVPTVTREVDMDSLFLLN
                     ASGEWLWEQLRQQTTLAQVAEKMAGHFRIEPARATADATAFVEQLVERNLVVAQPAS"
     gene            393..1526
                     /locus_tag="PKI64_10255"
     CDS             393..1526
                     /locus_tag="PKI64_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE01418.1"
                     /translation="MRDCDIGVGHSRVDAMRQRAREEGLPLHATLELTQRCNFRCVHC
                     YVLPNAASPTKELTAGEWLALVKEAADAGCFSVLLTGGEPLLRSDFPEIYLGIRKMGI
                     QVMIFTNASRVDQRIVEVLQEAPPRLIEITIYGASPQTYAAVTGRPEAYLEVMDGVQR
                     LRDAGMPVALKTILMNPNKHDFATIRAMRGAGERQVRYDAAIHARFPGDGEIQQYRVP
                     PAELVELEARHVPELQAEWERTAKREQTQAAKTAAAPGPQPLYTCAAGSISFYATATG
                     MIQPCVSAARYGVQWRPGKLLAAFRQSRQAIQSAFKPAGYACTGCAALPYCASCPAVA
                     ELETGIETAVFPYACQVAKTRQRRGVEKELAAKTSLVATPGIS"
     gene            1557..1733
                     /locus_tag="PKI64_10260"
     CDS             1557..1733
                     /locus_tag="PKI64_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01419.1"
                     /translation="MQEQEKNPQQDAYEAPRLEVVDLHGEELLAKSCKDITGAGPTLT
                     CQDQFGVTCSSLGS"
     gene            1730..2644
                     /locus_tag="PKI64_10265"
     CDS             1730..2644
                     /locus_tag="PKI64_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01420.1"
                     /translation="MRLDPAPAGSTSSGRNILDIAGLGIELAKPLCPDFSAWPDSYQA
                     FLNIRPDSTPADSVFLHGADVERPTDDTFTATFAGGAWQAGQANGRTVFRFNANDLPA
                     YLWAEPNADYSRVTVWPDPVHGRPMPPLLHPVDRVLCMGVLTHRQRIMVHACGWKYQG
                     QALLFPGVSGAGKTTICNQLMASGHGVILSDDRMVLRQTADGLDACGTPWPGDAKQAR
                     NESAPLRALCFIEKAAENRLTPIPPAEALKRLFTTASVPWFVPTMRDLALAVVEHLVA
                     TIPAYRLDFRPTPAVADCLLPLLTASHK"
     gene            2777..3865
                     /locus_tag="PKI64_10270"
     CDS             2777..3865
                     /locus_tag="PKI64_10270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE01421.1"
                     /translation="MKHFWRLIMVVAMVWGAGCGKKSAQVVKPNLINPDDVAVWVDQS
                     GITAGQVQREVNRLFAQVPANIPADQIPGIQARLVGEAVEGLIVRQLVRNEMERSKSI
                     ITREEIEKGKKDLEAALGPGGSLSVLLAETKLPMEEVEKNLQLDIFKNKMIKEQLDEA
                     LAGITEQVVREYYDTHPQEFTQPAGRLASHILLRVPQDATESVKADLRAKAEAIRQEL
                     LGGADFAQMAREKSECLSAERGGELGVIPRGREALSFEEAVYGQPFNEIGEVIETPVG
                     FHIVKVTGEQEEKLIPFEEVKGRLTEVLKLQAHQQVTANFIRGLREKATIKLVGPLAE
                     QAPAAPETPAEETAPALPPEAPVSVPAQ"
BASE COUNT          795 a         1164 c         1255 g          752 t
ORIGIN      
        1 ggcagtggcg gcggtggcag cgggggcggc agcgggggtg gcggcggtgg gggtgacgag
       61 tccccgttct aacgatcggc gaggtgtacc atgaagcttt ctcttgccca ccagcaattt
      121 caacagcgcc ccggtgtggt gctgcgtaaa gtggccggcg aagtcatgct ggttcccacc
      181 gtcacgcgcg aggtggatat ggacagccta tttttgctga atgccagcgg tgagtggctt
      241 tgggaacaac tgcgtcagca gactaccttg gctcaggtgg cggagaaaat ggccgggcat
      301 ttccggattg aacccgcgcg ggcgaccgcg gatgccacgg catttgttga gcagttggtg
      361 gaacgcaacc tggtggttgc ccaacctgca tcatgagaga ttgcgacatc ggagttggcc
      421 actcgcgcgt ggatgccatg cggcaacgcg cgcgcgagga ggggctcccg ttgcacgcca
      481 cactggaact gactcagcgc tgcaattttc gctgcgtcca ttgttatgtc ctgcccaacg
      541 cagcatcgcc gaccaaggaa ttgacagccg gcgaatggct ggcgctcgta aaagaggccg
      601 cagatgccgg ctgtttctcg gtgttgctaa ccggcgggga gccgctgttg cgctccgact
      661 tccccgagat ttatcttggt atccggaaaa tgggcattca ggtgatgatc ttcaccaacg
      721 cctcgcgtgt ggaccagcgg atcgttgaag tattgcagga ggcgccgccg cggctgattg
      781 aaattacgat ctacggtgcc tcgccgcaga cgtatgccgc ggtcaccggc cgtcccgagg
      841 cctatctcga agtcatggac ggtgtccaac gactgcggga tgccggaatg cccgtggcct
      901 tgaagaccat cctgatgaat cccaacaagc atgatttcgc gaccattcgc gccatgcgtg
      961 gggcagggga gcggcaagta cgttacgatg ccgcgatcca tgcccgcttc cccggcgacg
     1021 gtgagattca gcagtaccgc gtgccgcccg ccgagctggt ggagctggaa gcgcgccacg
     1081 ttcccgaact gcaggcggaa tgggagcgca ccgccaagcg ggaacaaacg caggcggcca
     1141 agacggcagc cgcgccgggc ccgcaaccgc tttacacatg cgcggccggg tccatttcgt
     1201 tctatgcaac cgccaccggg atgattcaac cgtgcgtcag cgccgcccgc tacggcgtgc
     1261 aatggcgccc cggcaagctg ctggcggctt ttcggcagtc ccgccaggcc atccagtcgg
     1321 cgttcaaacc cgccggttat gcctgcactg gttgcgcggc gctgccgtat tgcgcctcct
     1381 gcccggcggt agccgaactg gaaacgggca tagagaccgc cgttttcccc tatgcctgcc
     1441 aagtggccaa aacccgccag cggcgcggcg ttgaaaaaga gctggccgct aaaacaagtc
     1501 ttgttgcaac ccccggaata tcctaaaagt aatagtaatc ccccaaggag aaaagcatgc
     1561 aagagcaaga gaaaaatccc cagcaagatg cctacgaagc cccccgcttg gaagtggttg
     1621 atttgcacgg cgaagagctg ttggccaaga gctgcaaaga catcaccggc gctggcccga
     1681 cgctgacctg ccaagaccaa tttggcgtga cctgctcgtc attgggttca tgaggttgga
     1741 tcccgcgccc gccggctcca cgagctccgg acgcaatatc ttggacatag ccgggcttgg
     1801 cattgaactg gccaagcccc tatgtccaga tttttccgca tggcctgatt cctatcaggc
     1861 ctttcttaat attcggcctg attccacacc cgccgattcc gtgttcctcc atggcgctga
     1921 tgtagagcgc ccgacggatg acacttttac cgccaccttt gccggtggcg cctggcaggc
     1981 aggtcaggcg aacgggcgca ctgttttccg tttcaatgcc aacgacctgc ccgcctatct
     2041 ctgggcggaa cccaacgctg attattcgcg agtaaccgtc tggcccgatc ccgtgcatgg
     2101 ccggcccatg ccgccgcttc tgcacccggt ggaccgggtc ttgtgtatgg gggtgctgac
     2161 ccatcggcaa cggatcatgg ttcacgcctg cggatggaaa taccaagggc aagccttgct
     2221 gtttcccggt gtgtctggcg cgggcaaaac gaccatctgc aaccagctca tggcctccgg
     2281 ccacggggtt attttgtccg atgaccgcat ggtgttgcgg caaacggccg acggcctgga
     2341 cgcctgcggt acgccatggc ccggcgatgc caagcaggcc aggaatgaat cggcgccact
     2401 gcgcgccttg tgcttcatcg aaaaagccgc cgaaaatcgg ctgacgccaa tcccgcccgc
     2461 ggaagcattg aaacgacttt tcaccacggc ctccgtgcca tggttcgtgc ccaccatgcg
     2521 cgacttggcc ctcgcggtgg tggagcacct ggtggcgacg atacccgcct accggctgga
     2581 ttttcgcccc acaccggctg tggccgactg cctgctgccg ttattgacgg cttctcacaa
     2641 atagggccat gtggcccgtt tggatagggg aagaattctg ctggactcgg attgcagctt
     2701 gatttttgac gggtggggcg ggaaaatgag gcctcaaatc aatccgggcg tgacgcccgg
     2761 tgaaaggaag cagggtatga aacatttctg gcggctgatc atggtggtgg cgatggtctg
     2821 gggcgcgggc tgcgggaaga aaagcgcgca ggtggtcaaa cccaatctga tcaatccgga
     2881 cgatgtcgcg gtttgggtgg accaatcggg tattacggcg ggccaggtgc agcgggaagt
     2941 caatcggctt ttcgcgcagg tgccggcgaa cattcccgct gatcaaatcc cgggtattca
     3001 ggcgcgcctg gtgggcgagg ccgttgaagg cttgattgtt cggcaactgg tgcgcaacga
     3061 aatggaacgc tccaagtcca tcatcacccg cgaagaaatc gaaaagggga agaaagactt
     3121 ggaagccgca cttggccccg gaggttcgct gtccgtcttg ctggcggaaa ccaagctgcc
     3181 catggaagag gttgaaaaga atcttcaatt ggacatcttc aagaacaaga tgatcaagga
     3241 gcagctcgat gaagccttgg ccgggattac ggaacaggtc gtgcgggagt attatgatac
     3301 ccatccgcag gagttcaccc agccggccgg gcgcttggcg tcgcatatcc tgctgcgcgt
     3361 gccgcaggac gcgacggaat ccgtgaaggc ggatttgcgg gccaaggccg aggcgattcg
     3421 ccaagaactg ctgggtggtg ctgacttcgc gcaaatggcc cgggaaaagt ccgaatgtct
     3481 cagtgcggag cgtggcggcg aattgggtgt gattccgcgt ggccgcgaag cgctatcttt
     3541 tgaagaggcg gtgtatggcc agccattcaa cgaaatcggc gaagtcatcg aaacgccggt
     3601 gggtttccac atcgtcaaag tgacgggcga gcaagaagaa aagttgattc cctttgagga
     3661 ggtcaagggg cggctgacgg aggtgctcaa gctgcaagcc caccagcaag tcacggcaaa
     3721 ttttatccgc gggctgcgcg aaaaggccac gatcaagctg gtcgggccgc tggccgagca
     3781 agcccccgcg gctcctgaaa ccccggcaga agaaacggcg ccggccctgc cgccggaagc
     3841 tccggtctcg gtgccggctc aataatcccc acggaccgag tagtcaccgt ccaccggcag
     3901 ccggcttgtg cgactgccaa aagttgagct tgtcacccgt atgatcgctc cgtttccgga
     3961 ctccgc
//
LOCUS       DAOPNP010000152         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_25181_length_3908_cov_8.462237, whole genome shotgun sequence.
ACCESSION   DAOPNP010000152 DAOPNP010000000
VERSION     DAOPNP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25181_length_3908_cov_8.462237"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(643..1728)
                     /gene="gcvT"
                     /locus_tag="PKI64_10275"
     CDS             complement(643..1728)
                     /gene="gcvT"
                     /locus_tag="PKI64_10275"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOE01422.1"
                     /translation="MSTSLQRTPLYAELVAAGGRMVDFHGWELPVQFAGILAEHRAVR
                     TACGVFDVSHMGHIFVTGREAFRFVEWVNCNRMSAAPGRGVYAHILREDGGILDDAIT
                     FCLGPERFLAVVNAATTAKDWAWFQRQAAGFDVALENASDRLAMLAVQGPRAPALMAE
                     LFPAAATLPRFHAAEWPWAGETAIVMHTGYTGEDGFEVAASSAAIRELWAALLTRGAA
                     YGLQPCGLGARDTLRLEAGYLLYGQDADETRTPYEANCGWVVKLAKGDFMGRAGLLQR
                     PAAEIREKLVKLKLLDRGVPRHGCAVWQGDQAVGTLTSATFAPSLGVGIGLGYLPVAL
                     AGVGGRVEIEIHGKRVPAEVVEAFRPG"
     gene            complement(1755..3821)
                     /locus_tag="PKI64_10280"
     CDS             complement(1755..3821)
                     /locus_tag="PKI64_10280"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOE01423.1"
                     /translation="MRTQRTFWLNATVFLTGAGLMTVELVASRLIARYLGSSLYTWTG
                     VLGVILAGMSLGNYWGGRMADAARNLPGRIAAMFFASAAACLAILWLNAWAGNAAWLM
                     GLSWPVRILLHMAWAFLLPAAVLGTISPMVAKLAIERAAQTGRAIGTLYAWNAVGSIA
                     GTFLTGFYLTAWLGSVRIVVSMALLLAALGLLYAWAARAKGRGGVAGASAGLAIFLAA
                     FLLAVAPGEGVANVARSLKLRDEANEANAIFFAETQYQFLTVTAEPGNPQHRAFYQDK
                     LRHSEADLANLGDLKYPYMRIYDALVNQKLGRNSQPVRLLLLGGGGYVFPNYLFRARP
                     GSELVVVEIDPGVTRAAQEAFGLPPDAPLEIHHQDARNYVADALRQNRATPGSVPLFD
                     FIVCDSVSDYSVPYQLTTREFMAQVRELLKPDGVYVMNLIDAYDVGGFMNAVAQTFAE
                     VFPFRGAVSAVPELGPRNTTVFVGANQPLDLAGLPAAATRPESFQGRVLTEENFADLR
                     ARNGVCVLTDDYAPVENLLAPVVRADRLGLVTAALQRGIRAAEKKDYPRAVRHFQKAL
                     EIAPGHEDALTNLGHAQELAANESGALDAYSVLVQLYPENVNARNRAAMILARQGHLG
                     PALEQWRASLAVNPAQPDVLNNLGALAMQSGDRDRAVAYWRQALQYAPDAEILKRNLA
                     AAGSPE"
BASE COUNT          747 a         1445 c         1096 g          620 t
ORIGIN      
        1 gtggagggtt gcaggtttca agtttcaggc aatagccgaa cagcccgccg gggatgcaac
       61 cccggcctgc aaaacacatg gctgttaact gtagccgcgg gcggtgaccg cggggatttt
      121 ttccgggcac cggcctcagc gaggccggct acagtaagac agaaaatcca acagcccgcc
      181 gccggtacaa cggcggccta caggaagacc agcagccccg cgccacggag tggcgcgatt
      241 atgtagaggc catgccgggc cttgtctgct gtgcgcagca cgtgcatgcg gaggatttct
      301 tgcggatttt ggcggggctg ttgcgccaaa ataatcggtg gattttccga tgatggcgca
      361 ccgccgcaaa gcggcggaga ggtcagggtt cagagttcag aggtcaggta aaggccggca
      421 gccaacagcc ggagaaaatt aacgcaaagg acgcaatcgt cgcaaaagtc cgcaagcgat
      481 ttctatggga aaaattcttt gcgtgcttgg cgtcttggcg agagtcattc cctgcctcta
      541 gtctcaagtc tatagtctac agtctatagt ctagagcctc ttcttcgcgt ctcttgcgtc
      601 tcttgcgtta aaggccttcg gctgttcacc cgttcagccc cttcaccccg ggcggaacgc
      661 ctccaccact tcggcaggca cgcgcttgcc gtggatttca atctctaccc gccccccaac
      721 gccggccagc gccacgggca aatagccgag gccaatcccc accccgaggc tcggcgcaaa
      781 cgtcgcgctg gtcagcgtgc caaccgcctg atccccctgc cacaccgcgc agccatgacg
      841 cggcaccccc cggtccagca gcttcagctt caccaatttt tcgcggatct ccgccgctgg
      901 ccgctgcagc aagcctgcgc gccccatgaa atcgcccttg gccagcttga ccacccaacc
      961 gcaattcgcc tcgtagggcg tgcgggtttc gtcggcatcc tggccataga gcaaatagcc
     1021 cgcttccagc cgcagggtgt cgcgcgcgcc gaggccgcac ggttgcaggc cgtaggccgc
     1081 accgcgcgtc agcagcgccg cccacagctc ccggatcgcg gcggaggacg ccgccacctc
     1141 aaagccgtcc tcgccggtat atcccgtgtg catcacgatg gccgtctccc ccgcccacgg
     1201 ccattccgcg gcgtggaagc gcggcaacgt ggccgctgcc ggaaacagct ccgccatcag
     1261 cgcgggcgcc cgcggcccct gcacggccag catggccagc cggtcgctgg cgttctccag
     1321 cgccacgtca aaccccgccg cctgccgctg aaaccacgcc caatccttgg ccgtggtggc
     1381 cgcattcacc acggccagaa aacgctccgg cccgaggcag aacgtgatgg catcatccag
     1441 aatgccgcca tcctcgcgca aaatatgcgc atagaccccg cgccccggcg cggcgctcat
     1501 ccggttgcaa ttgacccact ccacaaaccg gaacgcctcg cgccccgtca cgaaaatatg
     1561 acccatgtgc gacacatcaa acacgccgca ggccgtgcgc accgcccgat gctccgccaa
     1621 gatgccggca aactgcaccg gcagctccca gccatgaaaa tccaccatcc ggccgccggc
     1681 ggccaccagt tcggcatata acggggtgcg ttgcagggac gtgctcatcg cggtggtggt
     1741 ggctcgcggg ggaactattc cgggctgccg gcggcagcca aattgcgttt gaggatttcc
     1801 gcgtccggcg cgtattgcag cgcctgccgc caataggcca cggcccggtc gcggtcgccc
     1861 gactgcatgg ccagcgcgcc gaggttgttc agcacatccg gctgcgcggg attcaccgcc
     1921 aggctcgcgc gccactgttc cagcgcgggg ccgaggtgtc cctgccgcgc caaaatcatc
     1981 gccgcgcggt tgcgtgcgtt gacattctcc ggataaagct gcaccaacac cgagtaggca
     2041 tccagcgcgc cggactcatt cgccgccagc tcttgcgcgt gacctagatt ggtcagcgcg
     2101 tcctcgtggc cgggggcaat ctccaacgcc ttttggaagt gccgcactgc ccgcggataa
     2161 tcctttttct ccgccgcgcg gataccgcgt tgcagcgccg ccgtcaccag tccgaggcgg
     2221 tccgcacgca ccaccggcgc cagcagattt tcgaccggcg cgtagtcatc cgtcagcacg
     2281 cacaccccgt tgcgcgcgcg cagatcggca aagttctcct ccgtcagcac ccggccttgg
     2341 aacgactccg ggcgtgtcgc tgccgccggc aggccagcga ggtccagcgg ctggttggcg
     2401 ccgacaaaca ccgtggtgtt gcgcggcccc agctccggca ccgccgacac cgccccccgg
     2461 aacgggaaca cctcggcaaa ggtctgcgcc actgcgttca tgaacccgcc cacgtcgtag
     2521 gcatcaatca ggttcatcac atacaccccg tctggcttga gcagctcgcg cacctgcgcc
     2581 atgaactcgc gcgtcgtcaa ctggtacggc acggaatagt cgctgaccga atcgcacaca
     2641 ataaaatcga acagcggcac gctgcccggc gtggcgcggt tttgccgcag ggcgtcggcc
     2701 acgtaattgc gcgcgtcctg atgatgtatt tcgagcggtg catccggcgg caggccaaac
     2761 gcttcttgcg ccgcgcgcgt cacgcccgga tcaatctcca ccacaaccag ctcgctgccc
     2821 ggacgggcgc ggaacaaata gttggggaaa acgtagcccc cgccccccag cagcagcagg
     2881 cgcaccggct ggctgttccg gcccagcttc tggttcacca gcgcgtcata gatgcgcata
     2941 tagggatatt tcaggtcgcc gagattcgcc aaatccgcct cgctgtgccg caatttgtcc
     3001 tgataaaacg cacggtgctg cgggttgccc ggttccgccg tcaccgtcag gaattggtat
     3061 tgcgtctcgg caaaaaagat cgcgttggcc tcattcgcct catcgcgcag cttgagcgac
     3121 cgcgccacat tcgccacgcc ctcacccggg gccaccgcca acaggaatgc cgccagaaaa
     3181 atcgccagcc ctgctgacgc ccccgccacg ccgccgcgtc ccttggcccg cgcggcccac
     3241 gcatagagca gccccagcgc cgccagcagc agcgccatgc tcactacaat ccgcacgctc
     3301 cccagccacg ccgtcaggta aaaccccgtc aggaacgtcc ccgcgatgct ccccaccgcg
     3361 ttccaggcat atagcgtgcc gatcgcccgc ccggtttgcg ccgcgcgttc aatcgccaac
     3421 ttcgccacca tcgggctaat cgtccccagc accgccgccg gcagcaggaa cgcccacgcc
     3481 atgtgcagca gaatccgcac cggccacgac agccccatca gccacgccgc attccccgcc
     3541 cacgcattca gccacaaaat cgccaggcac gccgccgccg acgcaaaaaa catcgctgcg
     3601 atccgccccg gcaaattacg cgccgcatcc gccatccgcc cgccccagta attccccaaa
     3661 ctcatccccg ccagaatcac ccccaaaact cccgtccacg tgtacagcga tgaccccaaa
     3721 tagcgcgcaa tcaagcgact cgccaccagc tccaccgtca tcaaccccgc gcccgtcaga
     3781 aacaccgtgg cattcagcca aaacgtccgt tgcgttctca tgacctggca ccatacccca
     3841 acgcccccgc ccgaatccag aacgaaggcg ttgcgctgcg cgcaaggaag gcgagagact
     3901 agagactg
//
LOCUS       DAOPNP010000153         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_25182_length_3908_cov_5.161692, whole genome shotgun sequence.
ACCESSION   DAOPNP010000153 DAOPNP010000000
VERSION     DAOPNP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25182_length_3908_cov_5.161692"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2711
                     /locus_tag="PKI64_10285"
     CDS             <1..2711
                     /locus_tag="PKI64_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOE01424.1"
                     /translation="EIKGMGGDPGRSQLEEKLGLQQKAVENLQKAVDAARTKLQGADT
                     DAQKLLAAKQLSGLETQLATAEAKALSLKNQLSAINYIKWGGMISGFGRAMQRMGQKF
                     TMYVGAPLAALGISSFNAFKDYELALARLKSALPDAGEEIEKLNQAALEMSETIPVSY
                     EEIMAIMTSLAKAGVPVGEIDGMTMALARMSAVTGMTAEEVGTSMVMFMNSMGLPMGN
                     VDQLGAALMQLADTSIATEADIFDMATRMAATGSLAGLSATEVLSLAAAFASMGVDAQ
                     AGGSAASKLMKQMQLAAETGKDVEGFAKVMGVSAEQFTAGWNQSPADSMLAFFQGLSD
                     MDTSGQQSVLAMLDEMGLTEIRLSNLVALGAKNPDMFESLMGTGAKGFEDNTALVKKA
                     GDIFNTTSGEMDMLGNSVRNAQADLGENVADTFQPLIDKVGELVGKFSELDEDTQTRW
                     VEIAGALVLLGPAAVGIGNVAKGIGSLVTFAGKIKSGEVTLFSKLIGALAGPAGGWLL
                     AAAGVAGVVAALNSIQTPAEQIIDGLKNIVIGLDEESYNTTMAALAEVKAQTDALSGE
                     KGEYNKNISAAVKRGHGTADMYGTALGYEAMLTQSQIAEIAGRYSDKYEELNAAIGAA
                     KTDADAKRAAEERDALKANWDAEVAQAKANYMAQVSALVSGMMQGQPEVKAALEQAAK
                     DYDLLAALENVTRQVMATDDMAAVEALWAGFFTPDIMNKYFEGQSIKNLAPPTAVDVL
                     RQELISSLKTSLEKAGGDESLAYTLLQSILTDPLAAGLYDATLTQGALDGLVELLDFK
                     NAAEQSGVDFGDALTPGLSDAITDSIPDVSGAMDSMQTQLVSQAAAMGAAVAAAFNNN
                     VNFRLPNAGGGGVNVNVNSPTAMDIYNIRKGLTDASRRAARGYGAG"
     gene            2708..3400
                     /locus_tag="PKI64_10290"
     CDS             2708..3400
                     /locus_tag="PKI64_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01425.1"
                     /translation="MSEITFKGTTASSKSVTVLKYPEIVKPTLRVETVKVPGRDGELT
                     LSGMPSYEAMVLECECMVPSVDKISAAAAWLTGRGDLVLGNDPDYAYDAQVIDEIRFE
                     KILRGHAHRRFTVPFLCQPLKKKATTEPNIELTAPGMVVNIGHVPSRPLIKIEGSGNV
                     VLAVGAYSLSITDINTSILIDSDLGMATNGTVNESYKVSGEWPLLVVGNNAVGWTGTV
                     SKVTITPRWRYL"
     gene            3397..>3908
                     /locus_tag="PKI64_10295"
     CDS             3397..>3908
                     /locus_tag="PKI64_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002399188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01426.1"
                     /translation="MIWIYEQGHEDFSTNGLGSLLPARCEIKEEQNGAYELELDHPID
                     KDGLFGLLKTGRVIKAPVPAQTTPIIRMPATTSYEVWETSAKAKVYTKKSTTLGSTIS
                     VTVPGIDLKSKKYDVVAVRGLTENEPKTVTKWQSNGNRVLEKVEAGIEVSVLEKDGAW
                     YKVTTPNGNTG"
BASE COUNT          923 a         1027 c         1270 g          688 t
ORIGIN      
        1 ctgagatcaa gggcatgggc ggcgacccag gccggagcca gctggaggag aagctggggc
       61 tgcagcagaa agctgtggag aacctgcaga aggcggtcga tgcggcccgg acgaagctgc
      121 aaggcgccga tacggacgcc cagaaactgc tggcggcgaa acagttgagt ggattggaaa
      181 cgcagttggc caccgccgag gcgaaggcgc tgtcgctgaa gaatcagcta tccgcgatta
      241 actatatcaa gtggggcggg atgatttcag ggtttggccg cgcgatgcag aggatggggc
      301 aaaagttcac catgtacgtc ggcgcaccgc tggccgcgct gggcatctcg tcgttcaacg
      361 cctttaagga ctatgaattg gcgctggcgc ggttgaagtc cgcgctgccg gatgctggag
      421 aggaaatcga gaaactcaat caggccgcgc ttgaaatgtc agaaaccatc ccggtcagtt
      481 acgaagaaat catggccatc atgacctcgt tggccaaggc cggcgttccg gttggcgaga
      541 ttgacggcat gaccatggcg ctggcccgga tgagcgccgt gaccgggatg accgccgagg
      601 aagtcggcac cagcatggtc atgtttatga actctatggg attacccatg ggcaacgtgg
      661 accaacttgg cgcagccctg atgcagctgg cggacacctc catcgcgacg gaagccgata
      721 ttttcgacat ggccacccgc atggcggcga ccggaagcct ggccggactg agcgcaactg
      781 aagttttgtc ccttgccgct gcgtttgcat caatgggcgt ggacgctcag gcgggtggtt
      841 ctgccgcatc caaactgatg aaacaaatgc agctggccgc cgaaacgggc aaagacgtgg
      901 agggctttgc caaagtcatg ggggtttccg ccgagcagtt taccgcggga tggaaccaat
      961 cgccggcgga ttcaatgctg gcattcttcc aggggctgtc tgatatggac acctccggtc
     1021 agcaaagcgt cctggcaatg ctggatgaaa tggggctgac cgagatccgg ctgtccaacc
     1081 ttgtagcgtt gggcgcgaaa aacccggaca tgtttgaaag cctgatggga accggcgcga
     1141 aggggttcga ggacaataca gccctggtca aaaaggccgg cgacatcttt aacaccacat
     1201 ccggggagat ggacatgctg ggcaactctg tccgcaacgc gcaggccgac ctgggcgaga
     1261 acgtggccga taccttccaa ccgcttattg acaaggtggg cgaactggtc ggaaagttct
     1321 ccgaattgga cgaagataca cagacccgat gggtggagat tgccggcgcg ttggtcctgc
     1381 tgggcccggc cgctgttggg attggcaatg tggcaaaggg tattgggtct ctggtgacct
     1441 tcgccggcaa aatcaaaagc ggcgaggtca cgctgttttc aaagcttatc ggcgcgctgg
     1501 caggaccggc gggtgggtgg ttattggccg cggccggggt ggccggagtc gttgctgcgc
     1561 tcaattctat tcaaacaccg gcagagcaga ttattgacgg cttgaaaaac atcgttattg
     1621 gcctggacga agaaagctac aatactacaa tggccgcgct ggcggaagtc aaggcccaga
     1681 ctgacgcgct gtcaggcgag aagggcgaat acaacaagaa tatatccgca gcggtgaaac
     1741 gcggtcatgg aacggctgac atgtatggca ccgcgctggg ctatgaagcg atgctcaccc
     1801 aatcccaaat tgccgaaatc gcggggagat attctgacaa atacgaggag ctgaacgcgg
     1861 ccatcggcgc cgcaaagact gacgcggatg caaagcgggc cgcggaagag cgtgacgcgc
     1921 tgaaagccaa ctgggacgca gaggtcgccc aagcaaaggc caactacatg gcacaggtca
     1981 gcgccctggt gagcgggatg atgcaggggc aacccgaagt gaaggccgcg ctggaacagg
     2041 ccgccaagga ctatgacctc ctggccgcgc tggagaacgt gacccgtcag gtcatggcga
     2101 ccgatgacat ggcggcggtt gaagcgctgt gggccggatt cttcacgccg gacatcatga
     2161 acaagtactt tgaaggtcag agcatcaaga accttgcgcc gccgaccgct gtggacgtgt
     2221 tacggcagga attgataagc tctctcaaaa catccctgga gaaagcgggc ggcgatgaat
     2281 cgcttgccta tactcttttg caatcaatct taactgaccc gctggccgcc ggactgtatg
     2341 acgcgacttt gacccaaggg gcgcttgacg gcctggtaga actgctggac ttcaagaacg
     2401 ccgcggaaca atccggggtg gactttggcg atgcgctcac tcctggcctc tccgacgcaa
     2461 tcaccgacag catcccggac gtatccggtg cgatggactc catgcagacc caacttgtat
     2521 cccaagcggc ggccatgggc gcggcggtgg cggcggcatt caacaacaat gtgaacttca
     2581 gactgcccaa cgcgggcggt ggcggcgtca atgtgaacgt gaacagcccg accgctatgg
     2641 acatctacaa tatacgcaag ggattgactg acgcgtcccg gcgtgcggca agggggtatg
     2701 gagccggatg agcgagatta cattcaaagg gacaaccgcg tcatcaaaga gcgtgacggt
     2761 gctgaaatat cctgagatcg tcaaaccgac cctgcgcgtg gagaccgtga aagtgccggg
     2821 cagggacggc gagctgacat taagcgggat gccgtcctat gaggcgatgg tgctggagtg
     2881 cgagtgcatg gtgccgagcg tggacaagat ttcggcggcc gcggcatggc tgaccgggcg
     2941 cggggacttg gtgctgggca atgacccgga ctatgcctat gacgcgcagg tgattgatga
     3001 aatcaggttc gagaaaatcc tgcgagggca tgcccacagg cggtttaccg tgccgttcct
     3061 gtgccagccg ctgaagaaga aggcgacaac tgaacccaac atagaattga ccgcgccggg
     3121 gatggtcgtc aacatcgggc atgttcccag ccgaccgctc attaaaattg agggcagcgg
     3181 gaacgtagtg ctggcggtgg gcgcatattc cctgtccatc actgatatta acacgtccat
     3241 cctgattgac agcgacctgg gcatggcgac caacgggaca gtgaacgaga gctacaaggt
     3301 gtcgggcgag tggccgctgc tggtggttgg gaataacgct gtgggctgga caggcacggt
     3361 cagcaaggtg accatcacgc cgaggtggag gtatttgtga tttggattta tgaacagggc
     3421 cacgaggatt tcagtaccaa tgggctgggg agcctcctcc ccgcccggtg cgaaatcaaa
     3481 gaggaacaga acggggccta cgaactggaa ctggaccatc ccatcgacaa ggatggtctt
     3541 tttggtctgc tgaaaacagg ccgggtcatc aaggcgccag tcccggccca gaccacccca
     3601 atcatccgga tgccggccac gacctcctat gaagtgtggg agacgagtgc taaggcgaaa
     3661 gtgtacacga agaaatcgac cacactgggg tcaaccatat ccgtgacagt tccggggatc
     3721 gatttgaaat ctaaaaaata cgacgtggtg gctgtccgcg gcctcacgga aaatgagccg
     3781 aagaccgtca ccaagtggca gtcaaacgga aaccgcgtgc tggagaaggt cgaagcgggc
     3841 atcgaagtat cggtgcttga aaaagacggc gcctggtaca aggtgacaac gcccaacggc
     3901 aacaccgg
//
LOCUS       DAOPNP010000154         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_27546_length_3652_cov_9.403392, whole genome shotgun sequence.
ACCESSION   DAOPNP010000154 DAOPNP010000000
VERSION     DAOPNP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27546_length_3652_cov_9.403392"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..572)
                     /locus_tag="PKI64_10300"
     CDS             complement(132..572)
                     /locus_tag="PKI64_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01427.1"
                     /translation="MSTVMKELKAEIARVARRVVRKEVAALQRSNAAHRGFIADLRRQ
                     ILNLQKEIKAQARGPAASELPVAGNAAAAKRFWITGKGIRALRKRFGMTQTEFAKLCA
                     VSIPTVVIWEATKGKVPVRRHATVARLQQVRQLTKRTARQALGA"
     gene            complement(848..1774)
                     /locus_tag="PKI64_10305"
     CDS             complement(848..1774)
                     /locus_tag="PKI64_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01428.1"
                     /translation="MHFSTLWKKSFHTVEKSLRLRHALLAALLASPFLAAAEPAEFAD
                     TPQLARELAANRQHNAAAIEYRRLALADPDSARAAGWFWLAAQQYASDHNPAQANRML
                     DRAEDTAPGSNAAPVAWLRAENALALRDWDSAGFYFDSLRLKADAPPWRDIAARGAAT
                     AALRRHDIPAARAALATSPTDPTSAITALDAYAARRDKKPWLGGLLGLVPGLGYAYSG
                     EYANATRSLILNSLFLWGMIETADDDQWAAFTVLTFVEFTWYSGSIYGGLDAAHRHNQ
                     RRLDAAITDIRGTPRAQPDFSKLPILVWQFEF"
     gene            complement(2092..2943)
                     /gene="yidD"
                     /locus_tag="PKI64_10310"
     CDS             complement(2092..2943)
                     /gene="yidD"
                     /locus_tag="PKI64_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOE01429.1"
                     /translation="MAITRPAARKIPGLVPGIFFCALALAAAAPPSLDLAAELFAEGQ
                     LASARLEARRALETATGPAADHARLLAATCALRLNDAPAAAKDELAALWQNPAAPLEL
                     RCQAAYELALADWHSPASRTPAALEFAYLHAQAAPLFWRAGCSLYFYLKAHKTLRREK
                     APLWQSLQTCRDAWPLEIWRECRPRRPHGPPLAARPAHWLVRFYRTHIGPAIGSRCDL
                     QPSCSEYFRQATQAHGLLGFPIMADRFCRESSVVAARAKPITMPDGRIRYADPLTDHT
                     FWLRGPP"
     gene            complement(2948..3238)
                     /locus_tag="PKI64_10315"
     CDS             complement(2948..3238)
                     /locus_tag="PKI64_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01430.1"
                     /translation="MKKIIATLLIGALVMIAVAPAASAANERGGVVGFFAGCCFGIRS
                     GAAYNDGKDLHWREWVLLIPIAGIVVAIMNGLDGANGLTSRDMASQYGSNFY"
     gene            complement(3269..3562)
                     /locus_tag="PKI64_10320"
     CDS             complement(3269..3562)
                     /locus_tag="PKI64_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01431.1"
                     /translation="MKKLVASLLIGLFVLMAVAPAASAAGQGRGGFVGFLAGCCFGIR
                     SGNAYNDGKELHFREWCRLIPYVNIVFMIWDGIDGAQGTTTAKLAQQYGATYY"
BASE COUNT          665 a          852 c         1435 g          700 t
ORIGIN      
        1 ttttgcaccc caagcaaacg cgctaccagt ctgcgctacg ccccgaccaa aggcgtgtat
       61 tcggcatggg atgtaccatg ccgggggatg ggcgtcaatg gcgcttttac cgcggggcgg
      121 cagggcgcag tttaggcgcc gagggcctgc cgggcggtgc gcttggtgag ttggcgcact
      181 tgctgcaagc gggcgacggt ggcgtgccgg cggacaggga ccttgccctt ggtggcttcc
      241 caaatgacga cggtgggaat actgacggcg cagagtttgg cgaattcggt ctgcgtcatg
      301 ccaaagcgtt tacgcagggc gcggatacct ttgccggtga tccaaaagcg cttggcggcg
      361 gcggcattgc cggccacggg gagttcgctg gcggccgggc cacgggcctg ggccttgatt
      421 tctttttgca ggttgagaat ctgccggcgc aaatccgcaa taaatccgcg gtgggccgcg
      481 ttgctgcgct gcagggccgc aacttccttg cgcaccacgc gccgggcgac acgggcgatt
      541 tcagctttca gttctttcat tacggtggac atggtttttc tcctatcaat atgcttacga
      601 taggttgaat ctagtggacc ccgcggaaag tgcaaggttt ttcgtatctt tttttattac
      661 gccgaaaaac ggctggttga tttttggtga ggattcagta gcaggacggc attgtgggcc
      721 gcgggagcgc ggagaaatat tgcaaagaat tatgttttga tttgattagg tggcgacgga
      781 ataaggcgtg gtgatttgct gcgaaaggcg cgggtggcgc ggggggatgg ggctgaggag
      841 tgatgaatta aaactcaaat tgccagacaa gaatgggcaa cttggagaaa tcgggttggg
      901 cgcggggggt tccccggatg tcggtaatgg cggcatcgag gcggcgttgg ttgtggcggt
      961 gggcggcatc gagtccgccg tagatgctgc cggagtacca ggtgaattcc acgaaggtga
     1021 ggacggtgaa ggcggcccac tggtcgtcat cggcggtttc gatcatgccc caaaggaaga
     1081 ggctgttgag gatgagggag cgggtggcgt tggcgtattc gccggagtag gcgtagccga
     1141 ggccggggac aaggccgaga aggccgccga gccacggttt tttgtcgcgg cgggcggcgt
     1201 aggcatcgag cgcggtgatg gcggaggtgg gatcagttgg agaggtggcg agggcggcgc
     1261 gggcggcggg gatgtcgtgg cgacgcaggg cggcggtggc ggcgccgcgc gcggcgatgt
     1321 cgcgccaagg gggggcgtcg gctttgaggc ggaggctgtc gaagtagaag ccggcggaat
     1381 cccagtcgcg cagggcgagt gcgttttcag cgcgcagcca agcgacgggc gcggcgttgc
     1441 tgccgggggc ggtgtcttcg gcgcggtcga gcatgcggtt ggcttgggcg ggattgtggt
     1501 cgctggcgta ttgttgggcg gccagccaga accagccggc ggcgcgggcg gaatcaggat
     1561 cggcgagggc caagcggcgg tattcgatgg cggcggcgtt gtgctgacgg ttggcggcga
     1621 gttcacgggc gagttgcggg gtgtcggcga attcagcggg ttcggcagcg gcgaggaacg
     1681 ggctggcgag gagcgcggcg agcagggcat ggcgcaggcg cagagatttt tccacagtgt
     1741 ggaaactttt tttccacagt gtggaaaaat gcacgaattt tttccacgct atggaaaaag
     1801 tttggagaaa ttttccacac aatggaacaa ctttgaagaa attttccaca caatggaaca
     1861 actttgcgga aaaagttcca cggtgtggaa aaatgttggt tggcggggat agaattttcc
     1921 acggtgtgga aacttttttt ccacagtgtg gaaaaatggg cggatttttt ccacgcaatg
     1981 gaaaaatccc gcgcacgcgg gataaacttt cagcgatttt tccacacaat ggcacaactt
     2041 tgcggaaaaa gttccatggt gtggaaaagg gtgtgggcgg gggggcggat ttcatggggg
     2101 acctcgtagc cagaaggtgt ggtcggtgag agggtcggcg tagcggatgc ggccgtcggg
     2161 catggtgatg ggtttggcgc gggcggcaac gacggaggat tcgcggcaga agcggtcggc
     2221 catgatgggg aagccaagga ggccatgggc ttgggtggct tggcggaagt attcggagca
     2281 actgggttgg aggtcgcagc gggagccgat ggcggggccg atgtgggtac ggtagaagcg
     2341 gacgagccag tgggcggggc gggcggcgag tggcgggccg tgggggcggc gggggcggca
     2401 ctcgcgccag atttcgaggg gccaggcgtc gcggcaggtt tggagggatt gccagagggg
     2461 ggccttttcg cggcggaggg ttttgtgggc tttgaggtag aagtacaggg agcagccggc
     2521 gcgccagaag aggggcgcgg cttgggcgtg gagataggcg aattcgaggg cggcgggggt
     2581 gcgggaggcg ggggaatgcc agtcggcaag ggcgagttcg taggcggctt ggcagcggag
     2641 ttcgaggggg gcggcgggat tttgccagag ggcggcgagt tcgtctttgg cggcggcggg
     2701 ggcgtcgttc aggcgcaggg cgcaggtggc ggccaggagg cgggcatggt cggcggcggg
     2761 tccggtggcg gtttccagcg cgcggcgggc ttccaggcgg gcggaggcca actggccctc
     2821 ggcaaatagt tcggcggcaa ggtcgaggga gggtggcgcg gcggcggcta gcgcaagggc
     2881 gcaaaaaaag attccgggca cgaggcccgg aatctttcgc gcggctggcc gcgtaatggc
     2941 catcggctta atagaagttg ctgccgtact ggctggccat gtcacgggag gtcaggccgt
     3001 tggcgccgtc caagccgttc atgatggcga ccacaatgcc ggcgatgggg atcaacagaa
     3061 cccattcgcg ccagtggagg tctttgccgt cgttgtaggc ggcgccggaa cggatgccga
     3121 agcagcagcc ggcaaagaag ccgaccacgc cgccgcgttc attcgcggcc gaagcggcgg
     3181 gagccaccgc gatcatgacc aacgcgccaa tcagcagggt tgcaatgatt tttttcatgg
     3241 ggaaagttcc ttcctgtttt cgatgggttt agtagtaggt ggcgccgtat tgctgggcga
     3301 gcttggcggt ggtggtgcct tgcgcgccat caatgccgtc ccaaatcatg aaaacgatgt
     3361 tcacgtaggg gatgaggcgg caccattcgc ggaagtggag ttccttgccg tcgttatagg
     3421 cgttgccgga gcggatgccg aagcagcagc cggccaggaa gccgacgaac ccgccacggc
     3481 cttggccagc agcgcttgcc gcgggagcca cggccatcag cacgaacagg ccaatcaaga
     3541 gacttgcgac caatttcttc atgtgcagtt gtccttttgg ggtgttgttt attcttacgg
     3601 ggcgaagctt atgctgtggc gcgggggaag tcaatcccac ggaaggcggg gg
//
LOCUS       DAOPNP010000155         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_28144_length_3592_cov_8.013005, whole genome shotgun sequence.
ACCESSION   DAOPNP010000155 DAOPNP010000000
VERSION     DAOPNP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28144_length_3592_cov_8.013005"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKI64_10325"
     CDS             complement(<1..749)
                     /locus_tag="PKI64_10325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE01432.1"
                     /translation="MTADLDTTIQTLGPCRHDSPLALNTSRGDYIGNFVSDRTRVRHQ
                     VEILPGSPPDSQIYFEKAGPRQKIFFDPAQTRAAIVTCGGLCPGLNNVIRSAFLELHH
                     NYGVREVLGIRHGFRGLTAQGEPPVPLTTAMVEDIHREGGTILSSSRGQQDINEMVEF
                     LRERQIDILICVGGDGTQKGTRAIAEEVTRRGWPIAVVGVPKTIDNDLQFVRQTFGYY
                     TAIEVAREILDGAHNESKGAPNGIGLVKLMGR"
     gene            complement(766..2040)
                     /locus_tag="PKI64_10330"
     CDS             complement(766..2040)
                     /locus_tag="PKI64_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01433.1"
                     /translation="MRVVLRQLLMLVASVAAWAAAAMAQAAPALLTVGPGWAWVRESF
                     PPSGEKALTRLVWTNPPAQCDLDTLQVWNVRRPWPMQDWHWQGAAPVAPAPDEPIRWQ
                     PPHRTPPPPSRPHVEIRLAEPMSDLLGHSLTYRLPGFNWQAFYRVIVRGIGPESIESV
                     QVDLDAFLHIQNNTAATFPDARVAIVGPDESLLPATKPFGLLALDYDNPLTDLWRNPP
                     APEPLVPAAYPLDVTASIPAHGRAEVRFARITRKPAQITHVCHSSVIHAPTPQGGRPL
                     QRVLQIPNTPAMGLGYALPPGRADLFLGAARGAPFQSGRVRHTPFPGTLELDLGTVET
                     VRATRQMGVEEPLPEGAKQVDYTITLTNGLTSPIRVQVIEQPLTPAQWNLVRSSVPCT
                     ISSRELTFNLTLPPHSARTITYRLRLSARPQS"
     gene            2134..2991
                     /locus_tag="PKI64_10335"
     CDS             2134..2991
                     /locus_tag="PKI64_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOE01434.1"
                     /translation="MSTIRPIQRIVTGTPQTDGAGVRLVRVISRRDVEDFDPFLMLDA
                     FDSRDPADYIRGFPWHPHRGIETVTYLVSGEIDHGDSLGNRGTIRDGGCQWMTAGSGI
                     IHQEMPQASPRMLGLQLWLNLPRKDKFTAPQYRDLSGDKIPRVQEDGVRVGVICGHYG
                     ATAGPTQGDYVRATMLDVEMAAGSTFRLPTDPAETVFIYILAGSGVVGEGDENLASHR
                     AVLLGSGDVFQARAGQDGMRLVLFAGRPLREPVAWGGPIVMNTPEELQQAFAEIENGT
                     FIRTSANPA"
     gene            2988..3371
                     /gene="folB"
                     /locus_tag="PKI64_10340"
     CDS             2988..3371
                     /gene="folB"
                     /locus_tag="PKI64_10340"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOE01435.1"
                     /translation="MKSRPKKLPPATTWIHLRDIEIRCILGIYPTERRTPRKVLVNIA
                     LATDITAAAKSDKIAHALNYEQIENDVRRIARQGRFFLIETLATRLADACLLYPAVRA
                     VRVTVDKPRALRHTRSVAIEILKSK"
BASE COUNT          713 a         1043 c         1144 g          692 t
ORIGIN      
        1 cgccccatca atttcaccaa gccgatgccg ttgggcgcgc ccttggattc gttgtgcgcg
       61 ccatccagta tttcgcgcgc aacttcaatg gcggtgtaat agccaaaagt ctgccgcaca
      121 aattgcagat cgttgtcaat cgtcttgggc acgcccacaa cagcgatcgg ccagccgcgg
      181 cgggttactt cttcagcgat ggcccgcgta cctttttgtg tgccgtcacc gccgacgcaa
      241 atcaggatgt caatttgccg ttcgcgaagg aattccacca tttcattgat gtcctgctgg
      301 ccgcgtgagg acgacaatat ggttcccccc tcgcggtgaa tatcctccac catggctgtg
      361 gtcaagggca ccggcggctc gccttgcgcg gtcaggccgc ggaacccatg ccggatgccg
      421 agcacttccc gcacgccata gttgtggtgc agttccagaa atgccgagcg aatcacattg
      481 ttgaggccgg ggcacaaccc gccgcaggtg acgatggctg cgcgggtctg ggccggatca
      541 aaaaagattt tttggcgcgg gccggctttt tcaaaataga tctgactgtc cggcggcgaa
      601 ccgggaagga tttccacttg gtggcgcacg cgggttctgt cggacacaaa gttgccgatg
      661 taatcgccgc ggctggtgtt gagtgccagc ggcgagtcgt ggcggcaggg gccaagggtc
      721 tggatggtgg tgtcgagatc ggcggtcatg gtgatttcct tttcgtcaag attggggacg
      781 ggcgctcaag cgcaggcggt aggtaatggt gcgggcggag tggggcggca aggtgaggtt
      841 gaatgtcaat tcgcgggagc tgatggtgca cggcacggac gaacgcacca aattccactg
      901 tgcgggggtg agcggctgct cgatcacttg cacgcggatt ggcgatgtca ggccgttggt
      961 gagcgtgatg gtgtagtcca cctgcttggc gccctcgggc aagggttctt ccacgcccat
     1021 ttggcgcgta gcgcgcacgg tttcgactgt gcccaagtcc aattccagtg tgccggggaa
     1081 gggcgtatgc cgcacgcggc ccgactgaaa tggcgcgccg cgcgccgcac ccagaaacaa
     1141 gtccgcacgg ccgggcggca gggcatagcc gaggcccatg gccggcgtgt tggggatttg
     1201 cagcacgcgt tggagcggtc ggccgccctg cggcgtgggg gcatggatga ccgaagaatg
     1261 gcaaacatgc gtgatttgcg ctggtttgcg cgtgatgcgc gcaaaacgga cttctgcccg
     1321 gccatgcgcc ggtatactgg cggtgacatc caacggatag gccgcgggga ccagcggttc
     1381 cggcgccggc ggattacgcc ataggtcggt caacggatta tcgtaatcca gcgcgagcag
     1441 gccaaaaggt ttggtggccg gcaggagcga ttcatccggg ccgacgattg ccacgcgcgc
     1501 gtcgggaaat gtggcggcgg tattgttttg aatatgcaga aaagcgtcca aatccacctg
     1561 cacggactca atggattccg ggccgatgcc gcggacaatc acccgataga acgcctgcca
     1621 gttgaagccc ggcaagcgat aggtcaacga atgccccagc agatcggaca tcggctccgc
     1681 cagccggatt tcgacatgcg ggcgggaggg ggggggcggg gtgcggtgcg ggggctgcca
     1741 gcggatgggt tcatccggcg ccggcgcgac gggcgccgcg ccctgccagt gccagtcctg
     1801 catgggccat gggcggcgga cattccacac ttgcagggtg tcgagatcac attgcgccgg
     1861 cgggtttgtc cagactagac gcgtgagcgc tttttcgccg gaaggcggga aagactcccg
     1921 cacccatgcc cagccggggc ccacggtgag caacgccggt gcggcttggg ccatcgccgc
     1981 ggctgcccac gccgcaaccg acgcgacaag catgagcaac tggcgcagga caactctcat
     2041 ggcgcgacag cataggccgc ccgttttgct gcggcaaggc gagaatcccg cgccaccgct
     2101 tgcattcgct cccggaggcg gtaggatgga cccatgagca ccattcgacc cattcaacgg
     2161 attgtcaccg gcacacccca gaccgatggc gcgggagtcc ggctcgtgcg cgtgattagc
     2221 cgccgcgatg tggaagactt tgatccattt ctcatgctgg atgcctttga ttcgcgcgat
     2281 cccgccgatt acatccgcgg cttcccgtgg catccgcatc ggggcatcga aaccgtgact
     2341 tacctcgtca gcggtgagat tgatcacggc gacagtctcg gcaaccgtgg caccatccgg
     2401 gacggcggct gccaatggat gacggccggc agtggcatca tccatcagga aatgccgcaa
     2461 gccagcccgc ggatgctggg gctgcaacta tggctcaact tgccgcgcaa ggacaagttc
     2521 accgcgccgc aatatcgcga cttgtccggc gataaaattc cccgcgtgca ggaagacggc
     2581 gtgcgcgtcg gcgtgatttg cggccattat ggcgctacgg ccgggccgac gcagggcgat
     2641 tatgtgcgcg ccaccatgct ggatgtggaa atggccgccg gcagcacatt ccgcctcccg
     2701 acggatcccg cggaaaccgt attcatctac attctggccg gctctggagt tgtgggggag
     2761 ggtgatgaaa atctggccag ccatcgtgcg gtattgctgg gcagcggcga tgtgtttcaa
     2821 gcgcgcgccg ggcaagacgg gatgcgtttg gtgttgtttg ccggacgccc gttgcgcgaa
     2881 cccgtggcat ggggcgggcc catcgtgatg aatacgcccg aagaattgca gcaggctttt
     2941 gctgaaattg aaaacggcac atttattcgg acatctgcca acccagcgtg aaatcccggc
     3001 ctaaaaaatt gccgccggcc accacttgga ttcacctgcg ggatattgag atccgctgca
     3061 tactggggat ttatcccacc gagcgccgca ccccgcgcaa ggtgctggtc aatatcgcgc
     3121 tggccaccga cattaccgcc gccgcaaaaa gcgacaaaat cgcgcacgcc ttaaattacg
     3181 agcagatcga aaacgatgtc cgtcggattg cccgccaagg acgctttttc ctgattgaaa
     3241 ccctcgccac gcggttggcc gatgcctgcc tgctctaccc cgctgtgcgg gctgtccggg
     3301 tcacggtgga caagccccgt gccctgcgtc acacccgcag cgtagccatc gaaatcctga
     3361 agtccaaata ggctcaaaag cagcgacttc tgaaaaccgg tttttgacat gccatccaga
     3421 gtttataaac tgtggataaa attatagtct taaaatatat acgcaatata ttgcggcata
     3481 agaagatgta atatatttga ttggcgagcc gattttctgg atgaagccat tccatccacc
     3541 tgagttcgac acttccaatc taagaagcca taacacattg ggatgcaatg ta
//
LOCUS       DAOPNP010000156         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_29714_length_3448_cov_7.511052, whole genome shotgun sequence.
ACCESSION   DAOPNP010000156 DAOPNP010000000
VERSION     DAOPNP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29714_length_3448_cov_7.511052"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKI64_10345"
     CDS             complement(<1..419)
                     /locus_tag="PKI64_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOE01436.1"
                     /translation="MKPTRLLPLLLLASLATFPPPARAQQADPAVPLAPGDALLVRID
                     NLGGHLPQYREIVDSDGNIELPFLGMLPAAGKTIAAVQAEMAAAYARNHLATNAAVTI
                     TYVTRFTPPPPPPHPIPPPDPPPPPPPPPPPPPPPTPP"
     gene            complement(437..2056)
                     /locus_tag="PKI64_10350"
     CDS             complement(437..2056)
                     /locus_tag="PKI64_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE01437.1"
                     /translation="MFSSELKFIVQLGILIVFTRIGGHWLRRWLRLPGVLGELIVGML
                     IGPYALGKISWPWVGAMFPPLNGAMPISTELYAVATFASIVLLFLSGLETDLSTFLRY
                     SVAGTIVGIGGVVFSFLLGAGCALWFGLADSWLDPHALFLGAVGTATSVGITARILSE
                     HRKMDSAEGVTVMAAAVFDDVLGVIILAIVVGTVRAETSGGPSWQHILWTAGKAFGFW
                     IVATVAGLLLARRFSRILKYFKTTQAISALALGCALLVAGLSEMAGLAMIIGAYIMGL
                     SLSRTDLVQVIQREIEGLHNILVPVFFATMGMLVNFAAVVPVFKIGLIYSLLAVISKV
                     AGCGLPALFCGFNRHGAIRIGTGMLPRGEVALIVAGIGLTSGAIGQDIFGIAILMTVI
                     TTVLGPLFLSASFENPAPGLRHPEDTTKTVAPPIVLPFPSADMADFMVSRIVTAFRNE
                     EFYVYRLTPEEPTYHIRKDDIFFILSRDGQEVRLTTSQRHATMARLLVAEEMLTLRSM
                     LHMATEDGDYASFEQNFVATLFQDSTPPPAE"
     gene            complement(2062..2508)
                     /locus_tag="PKI64_10355"
     CDS             complement(2062..2508)
                     /locus_tag="PKI64_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE01438.1"
                     /translation="MNLKKTLTAETISLNLQGTTKDAVLAELVDLLMASGHIRDRDAV
                     LKAVQEREKRMSTGMQNGIAIPHGKSDSVDCLVAALGIKRSGVDFGALDGQPSVIFVM
                     TVSPDSRTGPHIQFLAEISRPLNDPAVRAKLLAATTPDEVLHLLLG"
     gene            complement(2615..>3448)
                     /locus_tag="PKI64_10360"
     CDS             complement(2615..>3448)
                     /locus_tag="PKI64_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOE01439.1"
                     /translation="THAENTAPPYPVEQEKITPAQFADFSWWPRRPLGIFRFMNQAEV
                     VRRYLQDPRDVDFRDTGFPCITISREAGTDSHGIGRAIIMRLGQYADPDLNTGWDLYD
                     QKLCALIAQNKSLDADYDTLLTDKYRGGGVRRMVYEMLIGSSQEYRIQKKIDEVIHLL
                     ARLGKVVIIGRGGFHIARQLPSAIHLRLVAPWRHRLHNVMKYDNLSAVEAARKIKAID
                     RERTGFLKSYHRCNLHDPTNFDLIWNVGRGTTADLVNAVAELVHQRLGRLRKAGAIHA
                     P"
BASE COUNT          708 a          947 c         1203 g          590 t
ORIGIN      
        1 gggggggtgg gggggggggg gggggggggg gggggggggg gggggggggg ggggtctggg
       61 ggggggattg ggtggggggg gggggggggg ggggtgaagc gggtgacata ggtaatggtc
      121 acggcggcgt tggtggcaag gtggttccgg gcgtaggcgg cagccatttc ggcctggacc
      181 gcggcgatgg ttttgccggc ggcgggcagc attccgagga agggcagctc gatgttgccg
      241 tcgctgtcca cgatttcgcg gtactggggc aagtggccgc cgaggttatc aatgcggacg
      301 aggagggcat cgccgggggc cagcggcacg gccggatcgg cctgttgcgc gcgcgccggg
      361 ggcgggaacg tggcgaggct cgccagcagc agcagcggga gcaagcgggt gggtttcatt
      421 ggcgaaaagc cggcggctat tcggcgggcg gcggcgttga atcctggaaa agagtggcga
      481 caaagttttg ctcaaacgag gcataatcgc catcctcggt ggccatgtgc agcatgctgc
      541 gcagggtcag catttcttcg gccacgagca ggcgggccat ggtggcatgg cgctgggagg
      601 tcgttagccg tacctcctgg ccatcacggg agagaataaa aaagatatca tccttgcgga
      661 tatgataggt gggttcttcg ggcgtaaggc gatagacata gaattcttcg ttgcgaaaag
      721 cggtgacgat gcgcgacacc atgaagtcgg ccatgtcggc ggaagggaac ggcaggacga
      781 tgggcggcgc gacggtcttg gtggtgtctt ccggatggcg caggccgggc gccgggtttt
      841 caaagctggc ggacaagaac agcggaccga ggacggtggt gatgaccgtc atgagaatgg
      901 cgatgccaaa aatatcctgc ccgattgcgc cggaggtcag cccgatgccg gcgacaatca
      961 acgcgacttc gccgcgcggc agcatgccgg tgccaatgcg gatggcgcca tggcggttga
     1021 agccgcaaaa cagtgccggc aggccgcagc cggccacttt gctgatgacg gccagcagcg
     1081 agtagatcag gccgattttg aacactggca ccacggcggc aaagttgacc aacatgccca
     1141 tggtggcaaa aaagacgggc acaaggatat tgtgcaggcc ctcgatctcg cgctggatca
     1201 cctgcaccaa atccgtgcgc gagagcgaca gccccatgat gtaggccccg ataatcatgg
     1261 ccagcccggc catttccgag agacccgcca ccagcaacgc gcagcccagc gccagcgccg
     1321 agatggcctg cgtggttttg aaatacttga ggatgcgcga gaatcggcgc gccagcagca
     1381 agcccgccac cgtggccacg atccagaacc cgaacgcctt gccggcagtc caaagaatat
     1441 gctgccagga cgggccaccg ctggtttccg cgcggacagt gccgaccacg atggcgagaa
     1501 taatgacgcc cagcacgtca tcaaaaaccg cggcggccat gacggtgacg ccctccgccg
     1561 aatccatttt gcggtgttcg ctcaggatgc gcgcggtgat gcccaccgag gtggccgtgc
     1621 ccaccgcgcc aaggaacagc gcatgcggat ccagccagga atcggccagg ccaaaccaca
     1681 aggcgcaacc tgcgcccagc agaaacgaaa agaccacgcc accgatgccc acgatggttc
     1741 cggccactga gtagcgcagg aaggtggaca agtcggtttc caagccggac agaaacagca
     1801 ggacaatcga agcgaacgtg gcgacggcat acagttcggt cgaaatgggc atggcgccgt
     1861 tgagcggcgg gaacattgcg ccgacccacg gccaggatat tttgcccagc gcgtaggggc
     1921 cgatcagcat gcccacgatc agttcaccga gcacgcccgg caatcgcaac cagcggcgca
     1981 gccaatggcc gccaatccgc gtgaagacga tcagaatgcc aagttggacg ataaacttga
     2041 gttcagacga aaacatcccg ttcagcccag caacaggtgc agcacctcgt caggcgtggt
     2101 ggcggcgagc agcttggcgc gcacggcggg atcgttgagc gggcggctga tttccgccag
     2161 gaactggata tgcggcccgg tccggctgtc gggcgagacg gtcatgacaa aaatcacgga
     2221 aggttggcca tccagcgcgc cgaagtccac gccggagcgc ttgatgccaa gcgcggccac
     2281 gaggcaatcc acgctatccg acttgccatg cggaatggca atgccgtttt gcatgccggt
     2341 gctcatccgt ttttcgcgtt cttgcacggc cttgagcacg gcatcgcgat cccggatgtg
     2401 gccggaagcc atcaacaggt ccaccaactc ggccagcacg gcatctttcg tcgtgccttg
     2461 caggttcagg ctgatggtct cggcggtcag cgtctttttc aaattcatgt tctacactcc
     2521 aacccaaaaa acaaaactta cgccattctc ccctgtttgg agaccaattc aagcccaaac
     2581 gggaaacaaa aaaaagaggg ggggcagggg ggggctaggg ggcgtggatg gcaccggcct
     2641 tgcgcaggcg gccgagccgc tggtggacca gttcggcgac ggcgttcacc aaatcggccg
     2701 tggtgccgcg gcccacgttc caaatcaggt cgaaattagt ggggtcgtgc agattgcagc
     2761 ggtggtagct cttgaggaac ccggtacgtt cgcggtcaat ggccttgatt ttgcgggcgg
     2821 cctcaacggc ggagaggttg tcatacttca tgacattgtg caggcgatgc cgccaagggg
     2881 ccacgaggcg cagatggatg gcgctgggca gttggcgggc aatatggaag ccgccgcggc
     2941 caatgatgac caccttgccc agccgcgcga gcaggtggat gacttcatca atcttttttt
     3001 ggatgcggta ttcctgcgat gagccgatca gcatttcata gaccatgcgg cgcactccgc
     3061 cgccgcgata tttatcggtc agcagggtgt cgtaatcggc atcgaggctc ttgttttgcg
     3121 cgatgagcgc gcacagcttt tggtcataga gatcccagcc ggtgttgagg tccgggtcgg
     3181 cgtattggcc gaggcgcatg atgatggccc ggccaatgcc gtgggaatcg gtgccggctt
     3241 cgcgggagat ggtgatgcac ggaaagccgg tgtcccggaa gtccacatcg cgtggatcct
     3301 gcaggtagcg cctgaccact tcggcttggt tcataaagcg gaatataccc aatgggcggc
     3361 gcggccacca actgaaatcg gcaaattgcg ccggtgtgat tttttcttgt tctacggggt
     3421 aggggggagc agtattttcc gcatgagt
//
LOCUS       DAOPNP010000157         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_30372_length_3390_cov_8.104348, whole genome shotgun sequence.
ACCESSION   DAOPNP010000157 DAOPNP010000000
VERSION     DAOPNP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30372_length_3390_cov_8.104348"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1275
                     /gene="mtnA"
                     /locus_tag="PKI64_10365"
     CDS             169..1275
                     /gene="mtnA"
                     /locus_tag="PKI64_10365"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOE01440.1"
                     /translation="MNVQGKPYRTIWLAAPGCVRIIDQARLPHEFALLDLRTPAAMAA
                     AIRNMNLRGAGLIGCAAAWGIYLAVVAHAAADDFATRLATEAEALLATRPTAKNLEWA
                     VQRMQARLAGCSPAERLAVAQAEAQAITDEDAEFSRRMGQHGLAILRDIAARKGGAPV
                     NVLTHCNAGWLAFVDHGTALAPVYAAVAAGIPVHVWVDETRPRNQGAALTAWELAQHG
                     VPHTLVADNAGGHLMQHGLVDVVFVGADRVTAAGDVANKIGTYLKALAAHDNQVPFYA
                     VLPVSTIDWQIHDGVREIVIEERHADEVRTMTGWDDAAGRLTTVRICPAETPAANYGF
                     DVTPARLLTGIITERGLAPATREGLRQLYADLKP"
     gene            1256..1870
                     /locus_tag="PKI64_10370"
     CDS             1256..1870
                     /locus_tag="PKI64_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOE01441.1"
                     /translation="MPTSSHSAEGYIKFSFTHEPAPPPAHPTLAGLLRVRDELRRWGW
                     LGVLPDGIGYGNISARRPGGNTFVISASGTGRHKNALPEHFCAVLTVDIARNFVLCRG
                     PLPASSESMSHGAVYATRPDTHAVIHVHDRALYARLRATGAPATPDDAEFGTPELARA
                     IAALAPTLPPVAVLRLGGHPDGMLAFGPSIGAARDALAALRPKE"
     gene            complement(1874..2560)
                     /locus_tag="PKI64_10375"
     CDS             complement(1874..2560)
                     /locus_tag="PKI64_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE01442.1"
                     /translation="MAAAGQERLRLAGVSKSFPAAGGPVPVLHEVSLTLHAGEFGVIT
                     GPSGSGKTTLLMLAGLLLAPDSGGVWLDGVATAALPDAARAEIRKHDIGMVFQKFCLM
                     PHRSALDNVLFRFRYVGGDTRAARDQARDALARVGLAAKAGQAARLLSAGEMQRVAIA
                     RALARPAKILLADEPTGNLDPDSAAQVMELFRAVNRAGMAILLVTHNHDWIAHGTAHW
                     TMHNGRLQRQ"
     gene            complement(2560..3051)
                     /gene="ruvC"
                     /locus_tag="PKI64_10380"
     CDS             complement(2560..3051)
                     /gene="ruvC"
                     /locus_tag="PKI64_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOE01443.1"
                     /translation="MKVLGIDASLRGTGLAVIEQQGSRTRALHWEVIKIPAHVPHSGC
                     LRQISERLAAVLAAHQPAAAAIEGGFYFKNAKVALVLGEVRGVAIVACAMAAVPVYEY
                     SPRRAKQNITGWGAAPKEQVAKMVQSVLGLPTLPPADATDAFALALCHAQSGRGLLAG
                     EPI"
BASE COUNT          602 a         1078 c         1111 g          599 t
ORIGIN      
        1 gatttttcca cgctgtggaa aaaatatttc cacactgtgg aaaaaggtag cggatttttt
       61 ccacgctgtg gaaaaaagtt tatcccgcat agggggaatg ttccacggta tggaacgcac
      121 cggagcgcgg ccttgacgcg ggcgggggtt ttcggcgaca accacgccat gaacgtgcaa
      181 ggcaaaccgt atcggaccat ctggctggcc gcgcccggct gtgtgcgcat tattgatcag
      241 gcgcgcctgc cgcatgagtt tgcgctgctg gatttgcgca cgccggcggc catggccgcg
      301 gccatccgca acatgaactt gcgtggcgcg ggattgatcg gctgcgcggc ggcatggggg
      361 atttatctgg cggtggtggc ccacgcggcg gcagatgatt tcgccacacg actggcaaca
      421 gaggccgagg cgctactggc cacgcgcccc acagccaaaa atctggagtg ggccgtgcag
      481 cggatgcagg cccggctggc cggctgttcg ccggcggaac ggctggccgt ggcccaggcc
      541 gaggcgcagg ccatcacgga tgaggatgcc gagttttccc gccgcatggg ccagcacggg
      601 ctggccatcc tgcgcgatat cgccgcgcgc aagggcggcg cgccggtgaa tgtgctcacc
      661 cactgcaatg cgggctggct ggcatttgtg gatcatggca cggcgctggc gccggtgtat
      721 gccgcggtgg cggcgggtat ccccgtgcac gtgtgggtgg atgaaacccg cccgcgcaac
      781 cagggcgccg cgctgacggc atgggagctg gcgcagcacg gcgtgccgca tacgctggtg
      841 gcggataacg cgggcgggca cctcatgcag cacggcctcg tggatgtggt gtttgtgggc
      901 gcggaccgcg tgacggccgc gggcgatgtg gcgaacaaaa tcggcaccta tctcaaggcg
      961 ctggccgcgc acgacaacca agtgccgttc tatgccgttc tgcccgtgtc caccattgat
     1021 tggcaaatcc acgacggcgt gcgcgaaatt gtgattgaag aacgtcatgc cgatgaagtc
     1081 cgcaccatga ccggctggga tgacgccgcc gggcggctga ccaccgtgcg gatttgcccc
     1141 gccgaaaccc ccgccgccaa ctacgggttt gatgtcacgc ccgcgcggct gctcacgggc
     1201 atcatcaccg aacgcgggct ggcgcccgcc acccgcgaag gattgcgcca actgtatgcc
     1261 gacctcaagc catagcgccg aaggctacat caagttttcc ttcacgcacg aacccgcgcc
     1321 gccccccgcc caccccacgc tggccggact gttgcgcgtg cgcgacgagc tgcgccgctg
     1381 gggctggctg ggcgtgctgc ccgacggcat cggctacggc aacatcagcg cgcgccggcc
     1441 gggcggcaat acgtttgtga tcagcgccag cggcacgggc cgccataaaa acgcgctgcc
     1501 ggagcatttt tgcgcggtgc tgacggtgga catcgcgcgc aactttgtgt tgtgccgcgg
     1561 gccgctgccg gcttcgtcgg aatccatgag ccacggcgcg gtctatgcca cgcggcccga
     1621 tacgcacgcg gtcattcacg ttcacgaccg cgcactctac gcgcggctgc gcgccacggg
     1681 cgcgccggcc acgccggacg atgctgaatt cggcacgccg gaactggcgc gggccatcgc
     1741 cgcgttggcc cccaccctgc cgccggtggc ggtgctgcgg ttgggtggtc acccggatgg
     1801 gatgctggcg ttcgggccga gcattggcgc ggcacgcgat gcgttggccg cactgcggcc
     1861 aaaggaatag ggcctattgc cgctgcaagc ggccgttgtg catggtccaa tgcgcggtgc
     1921 cgtgcgcgat ccagtcgtgg ttgtgcgtca ccagcaggat ggccatgccg gcgcggttca
     1981 ccgcgcggaa gagttccatg acctgtgcgg cggaatcggg gtccagattc ccggtgggtt
     2041 cgtcggccag caaaatcttc gcggggcggg ccaacgcgcg cgcgatggcc acgcgctgca
     2101 tttcgccggc ggagagcaac cgcgcggcct gcccggcttt ggcggccagc cccacgcggg
     2161 ccagcgcatc gcgggcctga tcgcgggcgg cacgggtgtc gccaccgaca tagcgaaagc
     2221 ggaagagcac gttgtcgagc gcgctgcggt gcggcatgag gcaaaacttt tggaacacca
     2281 tgccgatatc gtgcttgcgg atctcggccc gcgcggcatc tggcagggcc gccgtggcca
     2341 cgccatccag ccagaccccg ccgctatccg gcgccagcag caggccggcc agcatcagca
     2401 gtgtggtttt gccggaaccg gacggaccgg taatgacacc aaactcaccg gcgtgcagcg
     2461 tgagtgatac ttcgtgcagc acgggcaccg gcccgccggc ggcggggaaa gatttggaca
     2521 cccctgccag ccgtagccgt tcctgacctg cagcggccat cagatgggct cgcccgccaa
     2581 caggccgcgt ccgctttgcg catggcagag cgccagcgcg aaggcgtcgg tggcatcggc
     2641 cggcggcagc gtgggcaggc ccagcaccga ttgcaccatc ttggccacct gctccttggg
     2701 cgcggcaccc cacccggtga tgttttgctt ggcacggcgc ggcgaatatt catagacggg
     2761 caccgccgcc atggcgcagg cgacaatggc cacgccgcgc acctcgccca gcaccagggc
     2821 cactttggca ttcttgaaat aaaaaccacc ttcgatggcg gccgcggcgg gctggtgcgc
     2881 agccagcacg gcggcgagac gctcgctgat ttgccgcaaa cagccggaat gcggcacgtg
     2941 ggcgggaatc tttatcactt cccaatggag cgcgcgcgtg cgtgagcctt gctgctcaat
     3001 caccgccaac cccgtgcccc ggagcgaagc atctatgccc agaactttca tgcgggtaat
     3061 ctaccgcgaa acccaccgca aaaaaaagcg ccaaaaccct acccgctccg ggaaggatcg
     3121 ggattgatga aaagatcctc ttggatgcac cctttttttt cgcttttctc agtgacagga
     3181 tcgtctcgtt tgggatgtgg aagcagccgt tttcagatca cttccttttt cccatttgaa
     3241 ccgtactgtc cagagtttat aaagtgtgga catatattgt aatattcatt tctcaatcac
     3301 atacaatttg ttacgtctat ttattagggc atttatgatt agctattttg acgtcaatgt
     3361 ccacagttta taaactctgg acaaaattaa
//
LOCUS       DAOPNP010000158         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_30658_length_3365_cov_9.604230, whole genome shotgun sequence.
ACCESSION   DAOPNP010000158 DAOPNP010000000
VERSION     DAOPNP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30658_length_3365_cov_9.604230"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1059)
                     /locus_tag="PKI64_10385"
     CDS             complement(151..1059)
                     /locus_tag="PKI64_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01444.1"
                     /translation="MRQLNQVRTWQGLAVALWVMAAVLGVHLAWRGWRSAQPAAPGRA
                     RAVPLPPAFQWPAVDPAAWRVFRSGAPVAPPPVAGALSARYRLAGVFLILTEPGADTD
                     TQRCAILDDLQTREQILAQEGEHAGDARVVRVTPDYVILSSGEHEETITLAAGSAGDP
                     EKPTVSAAATEPMPTLETTRFGRRVGDTRWEVNRQAVLAYYQEMMDNPERLAGLFMAM
                     EPDRDANGRVAGYRLNTALGEKDFYNQVGLRDGDVVRRVNSMRMTSQRRAEYFIGEFV
                     QNRLGAVVLDIERDGQPQKLIYLVQE"
     gene            complement(1097..1484)
                     /gene="rnpB"
                     /locus_tag="PKI64_10390"
     ncRNA           complement(1097..1484)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI64_10390"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1522..2202)
                     /locus_tag="PKI64_10395"
     CDS             complement(1522..2202)
                     /locus_tag="PKI64_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOE01445.1"
                     /translation="MALQACDQEIRRLRKEEADIPLRKQQIEARLNAHQEGLAQAERA
                     LLEARTRIKQLEAETEAGHQQIQKFRDQQLQIKTNDGYKALEKEIATTQQKIRGLEDQ
                     TLTTMEAMEEAQGVVQTRREALAKEQERVAQEIQALEARRGAIADDLQRAEAERITKA
                     ADIAPDWLARYERIFAKQHDAAIALVERGTCGGCHMKLSPAQILDARKPDTLTLCVHC
                     SRLLYLAP"
     gene            2389..>3365
                     /locus_tag="PKI64_10400"
     CDS             2389..>3365
                     /locus_tag="PKI64_10400"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOE01446.1"
                     /translation="MVHTVLITRDYDHLSEVAAQLIEQRLAATLAARGSAVLGLATGS
                     SPTGAYKHLAKACNAGRLDAAKIRSFNLDEYIGLPGANAQARSLHPESYTFFMIQELF
                     GLLRRKFAAAHVPAGCLIEQDKLEAELTAHPGDWQEAGADKGRAILIRPTAESAYLRW
                     IRTDVLDAYEQDIRRAGGIDLHVIGVGGRGHVAFHECGIPFAGNRMLLVKLDDNTVAN
                     AVSDHHFARAADSPRFAISMGAELVYEAKSVVLLASGARKAEPMAAALAEAPSPAVPI
                     SYGQLYAQRGGEMIYVIDRAAATGVLDRRNEIIARGIEIRDLSNAAATRP"
BASE COUNT          569 a         1069 c         1039 g          688 t
ORIGIN      
        1 ctctcgatca agaaaacact tcctcgatcc gctaaccttt atatgcgaaa acgccgggga
       61 accaaagttt tttttgtgtt aaaaatgcaa ccgttttact acaggggaat tgaccacatt
      121 ttaccaaggc gctgaatttt ttccggcggc tcattcctgg acgagataga ttagtttctg
      181 cggttggcca tcgcgttcga tgtcgagcac gacggcgccc aagcggtttt gcacaaactc
      241 gccaatgaaa tattccgcgc ggcgctggct ggtcatgcgc atggaattga cgcgccgcac
      301 aacatcgcca tcgcgcaggc cgacttggtt gtaaaaatct ttttcgccga gcgcggtgtt
      361 caatcggtag ccggcaaccc gaccattggc atcacgatcc ggctccatgg ccatgaagag
      421 cccggcaagg cgttcggggt tgtccatcat ttcctgatag taggcaagta cagcttggcg
      481 gttcacttcc cagcgggtgt cgcccacacg gcggccgaag cgggtggttt ccaacgtcgg
      541 catgggttcc gtcgcggcgg cgctgacggt tggtttttcc gggtcaccgg cggagccggc
      601 ggcgagcgta atggtttctt cgtgctcgcc actggaaagg atcacgtagt cgggggtcac
      661 gcgcaccacg cgggcatcgc cggcgtgctc gccttcctgc gcgaggattt gttcccgggt
      721 ttgcaggtca tcgagaatgg cgcaacgttg ggtgtcggtg tccgcgccgg gctcggtcaa
      781 aatcaaaaac acgccggcca gccggtagcg cgcagagagc gcgccggcca ccggcggtgg
      841 ggccacgggt gcgccggagc gaaacacgcg ccacgccgct gggtccaccg caggccactg
      901 gaaggccggc ggcaatggaa cggcgcgcgc gcggccgggc gcggcgggtt gcgccgaacg
      961 ccaaccgcgc caagccaagt gcacgcccaa cacggcggcc atgacccaca aggccacggc
     1021 caatccttgc catgtgcgga cctgattcaa ctgcctcatc tgctgcattc tttttttgag
     1081 ttggttcatt gacaaccgga gctccggacg aataagccgg attctgtcag ccgcccgatg
     1141 gcggctccga tcatttatct aggcgatcaa cccggaatcg ccgcgcgttg ccgcgcctcg
     1201 ggacgggccg cccctgcgat tccctatttg atcttgctcc gggcggggtt tgccttgcgc
     1261 agcgcctctc ggcatccgca cggtggtctc ttacaccgcc gtttcaccct taccggcgcg
     1321 cggcgcgagc cgcggccggc ggtttggttt ctgtggcact ttcctttccc ttcgtttgcc
     1381 aaagggattc cgcacctccc ggcgcggacg ccctgcccga tggagtccgg actttcctcc
     1441 ggccggcacg ctgacgcgcc cgaccggcga ccggcgcccg gagccccggc tgtcaaagaa
     1501 cacgagggaa gttataaggg attatggggc cagatacaac aagcggctgc aatgcacgca
     1561 aagggtcagg gtatcgggct tgcgggcatc caggatctgg gcgggcgaga gtttcatgtg
     1621 gcagccgccg caggtgccgc gctccacgag ggcaatggcg gcatcgtgct gcttggcgaa
     1681 aatgcgttca tagcgggcca gccaatcggg ggcgatatcg gcggctttgg tgatccgttc
     1741 ggcttcggcg cgttgcaggt catcggcgat ggcgccgcgg cgggcctcca gcgcctgaat
     1801 ttcctgcgcc acgcgttcct gctccttggc cagcgcctcg cgccgggtct gcaccacgcc
     1861 ctgcgcttct tccatggcct ccatggtggt cagggtttga tcttccagcc cgcggatttt
     1921 ctgctgggtg gtggcgattt ctttttcgag cgccttgtaa ccatcgttgg ttttgatctg
     1981 caactgttgg tcgcggaact tttggatttg ctggtggccg gcctcggttt cggcttccaa
     2041 ctgcttgatg cgcgtgcggg cctcgagcag ggcgcgttcc gcctgcgcca acccttcctg
     2101 gtgcgcgttc aggcgcgcct caatctgctg cttgcgcagc gggatatctg cttcttcctt
     2161 gcgcaagcgc cggatttcct ggtcgcatgc ttgcaaggcc aataattgct ccatcgcctt
     2221 tgccactcgt ccgcctcgtc tttctggttg ccggaaaaga actatccaat tcttatactc
     2281 cgcgcgcggg gggctggcaa gcacgccctt gccaagaaaa acggattgca ttccggcggc
     2341 ccaaagcgca cctttccggg caatcaaacc acatatgttg aattgcctat ggtgcacacc
     2401 gtcctcatta cccgcgatta cgaccacctg agcgaggtgg cggcccagct tattgaacag
     2461 cgtttggcgg ctacgctggc cgcccgcggc tccgcggtac tgggcctggc caccggcagc
     2521 tcccccaccg gcgcttacaa gcacttggcc aaggcctgca atgccgggcg gctggatgcc
     2581 gccaaaatcc gcagcttcaa tctggatgaa tatatcggcc tgcccggcgc caatgcccag
     2641 gcccgctcgc tgcatccgga gagctacacg tttttcatga ttcaggagtt gttcggcctg
     2701 ttgcgccgca agtttgctgc cgcccacgta cccgccggct gcctgatcga gcaggacaaa
     2761 ttggaggcgg aactgaccgc ccaccccggc gactggcaag aagcaggcgc cgataaaggt
     2821 cgggccatcc tcatccgccc cacggccgaa tcggcctatc tgcgctggat ccgcaccgat
     2881 gtcctggatg cctatgaaca ggacattcgg cgtgccggcg gcattgatct gcatgtgatt
     2941 ggggtcggcg ggcgcggcca tgtggcgttc cacgaatgcg ggattccttt tgccggcaac
     3001 cggatgctgc tggtcaagct ggacgacaat accgtggcca acgccgtgag cgaccaccac
     3061 tttgcccgcg cggcggacag cccgcgcttc gctatttcga tgggcgcgga gctcgtgtac
     3121 gaggctaaga gcgtggtgtt gttggcttcc ggcgcgcgca aggcggaacc gatggccgcc
     3181 gcgctggccg aagccccctc ccccgccgtg ccaatttcct acgggcagtt atacgcccag
     3241 cgcggcggcg aaatgattta tgtgattgac cgcgccgcgg ccacaggtgt gctggatcgc
     3301 cgcaatgaaa tcatcgcacg cggaattgaa atccgcgatt tgagcaatgc cgccgccacc
     3361 cgccc
//
LOCUS       DAOPNP010000159         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_31556_length_3294_cov_8.044149, whole genome shotgun sequence.
ACCESSION   DAOPNP010000159 DAOPNP010000000
VERSION     DAOPNP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31556_length_3294_cov_8.044149"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKI64_10405"
     CDS             complement(<1..320)
                     /locus_tag="PKI64_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01447.1"
                     /translation="MKRTQWNGITILLIAALIAVALPAPAAVAGDMGGDSVGAAITIG
                     LMATIVVVYGLVSLRADVDRYTATPAETMARAAKMADESPIVLQTISAPAQEVAGASL
                     GLRLT"
     gene            complement(346..1329)
                     /locus_tag="PKI64_10410"
     CDS             complement(346..1329)
                     /locus_tag="PKI64_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOE01448.1"
                     /translation="MKFKKQIVIEVVVGIFSFAVIAVLFALTTMLSEEVLFRQYTYLD
                     VVFNSVNGLRVGDEVNARGVTVGKVKGIALAPDGVHIRVRLDQPLELREDYRIAVMAG
                     SVLGGRFLQVEQGSPDAPVISLDQTLHGSEAAELLDSATRTVEDIRNALNDGVLADLK
                     ASMAEIRKITTNLAEGQGTLTRLLNDEQLYGEIQQIAANIRTISGAIADGEGTIGKLV
                     MDDDIYAQVKAVAANLNDISARLANGEGTVGRLLSEDDQVYQDLAATIKEIRGIAESV
                     GRGEGSVGKLLADEELYQQIQALVREGRAALDDLRETSPVTTFTSIFFGAF"
     gene            complement(1326..2090)
                     /locus_tag="PKI64_10415"
     CDS             complement(1326..2090)
                     /locus_tag="PKI64_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE01449.1"
                     /translation="MIRFENVTKRFGRKTVLDDLSFEIQRGETFVIVGLSGTGKSVSL
                     RHMVRLLTPESGRVWIGDDCVSEANGSELQRILQRFGVLFQGAALLQWLNVLDNVCLP
                     LRQTRRLPEAEILRLAEEKLEMVGLTGAAEKMPADISGGMRKRVGLARAIVTQPEIVL
                     YDEPTSGLDPVISRQIDLLIDDLRRRLGVTSVVVTHDLNSALYIGTRVAMLHEGRMVV
                     CATPEEFIASKQPVVRDFLQAQGIADLQDVRKAFGK"
     gene            complement(2087..2899)
                     /locus_tag="PKI64_10420"
     CDS             complement(2087..2899)
                     /locus_tag="PKI64_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE01450.1"
                     /translation="MITREPPYLEPPVNAIAALGRRVIASCQGSGRALMMLLGAVKYL
                     PFMFSRRNRADVMQQCYLVGIKSLGVVSVVGLFTGMILALQLGLALRTFGQEWTIGTL
                     VCNAMLREMGPFMTGLVVAASVGSAIAAQIGTMTVSEEIAALDVMGIDPLRYLMMPRL
                     VALAAMTPLLALYMDMIGLFGGSVVGATMLGVSYESYFDGAFRYTHNKDLYVGLLKAF
                     IFGVIVVCVSCYQGFITGEGAVGVGRATRRTVVVSFLLILTLGYFVTRLAYR"
     gene            complement(2896..>3294)
                     /locus_tag="PKI64_10425"
     CDS             complement(2896..>3294)
                     /locus_tag="PKI64_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01451.1"
                     /translation="WPLFSRQADVDAQTLRWRWLDLWPAGQPPAVERSWAPLWTLWDY
                     RAQQDSRDLDVLWGLYRQTSRAEEARAFSLFPLWRHERAARNEARRWSVLKGLLAYDR
                     TATNRQVRVLWLGRIPLKPAPTAAAAELEP"
BASE COUNT          655 a         1079 c          985 g          575 t
ORIGIN      
        1 gtcaggcgca ggccgaggga ggcgccggcg acttcctggg cgggagcgct gatggtttgc
       61 aggacaatgg gggattcatc ggccattttg gcggcgcgcg ccatggtttc ggccggggtg
      121 gccgtgtagc gatcaacatc ggcgcgcaaa gacacgaggc catacaccac cacgatcgtg
      181 gccatgaggc cgatggtgat ggcggcgcca acggaatcgc cgcccatgtc accggcaacc
      241 gcggcgggtg cgggcagggc cacggcaatc aaggccgcga tcagcaggat cgtaatacca
      301 ttccattggg tgcgtttcat gggtgtgcct ccgcgcttgt atggcctaaa atgctccgaa
      361 gaaaatactg gtgaaggtgg tgaccggcga agtttcacgc aggtcgtcca aggcggcgcg
      421 cccctcgcgc accaaggcct gaatctgctg gtacagttct tcatcggcca gcagtttgcc
      481 gacggagcct tcgccgcgcc cgacgctttc ggcaatgccg cggatttctt tgatcgtggc
      541 ggccaggtcc tgataaacct gatcatcctc gctgagaagc cggccaacgg tgccttcgcc
      601 attggcaagg cgggcgctga tatcgttcaa gttggcggcg acggctttca cctgcgcgta
      661 gatgtcgtca tccatgacca gcttgccgat ggtgccctcg ccatcggcaa tcgccccgct
      721 aatcgtgcgg atgttggcgg caatctgctg gatttcgccg tacagttgtt catcgttcag
      781 caggcgggtc agcgtcccct gcccctcggc gagattcgtg gtgattttgc ggatttcggc
      841 catggaggcc ttgaggtcgg ccagcacgcc gtcgttgagc gcattgcgga tgtcctccac
      901 cgtgcgggtg gcgctatcca gcagttcagc ggcctcggac ccgtgcaggg tttggtccag
      961 cgaaattacg ggagcatcgg gtgaaccctg ctccacctgc aagaaacggc cgcccagcac
     1021 ggaaccggcc atcacggcga tccggtaatc ctcgcgcaat tccagcggct gatccaaccg
     1081 cacgcggata tgcacgccat cgggcgcgag cgcgatgccc ttcaccttgc cgacggtgac
     1141 cccgcgcgcg ttgacttcat cgcccacgcg caggccgttg acggaattga acaccacgtc
     1201 caaatacgtg tattgccgga acaacacttc ctcgctgagc atggtggtca gcgcaaacag
     1261 cacggcaatg acggcaaacg aaaaaatgcc gacgaccact tcaatcacaa tttgtttttt
     1321 gaacttcatt ttccaaacgc ctttcggacg tcctgcaagt ccgcgatgcc ctgcgcttgc
     1381 aggaagtctc gcacgaccgg ttgttttgat gcaatgaatt cctcgggggt ggcgcaaacg
     1441 accatgcgcc cctcgtggag catggccacg cgcgtgccga tatagagagc actattcaaa
     1501 tcgtgggtga ccaccacgct ggtcacaccg agccgccgcc gcagatcgtc aatcagcagg
     1561 tcaatttgcc gcgaaatgac gggatcgagc ccggaagtcg gttcgtcata gagcacgatt
     1621 tccggctggg tgacgatggc gcgggccaaa ccgacgcgct tgcgcatgcc gccggagata
     1681 tccgccggca ttttttcggc ggcgccggtc aggccgacca tttccagttt ttcttcggcg
     1741 aggcggagta tttctgcctc aggcaggcgg cgggtttggc gcagcggcag gcagacgtta
     1801 tccaacacat tcagccactg cagcagggcc gcgccctgga acagcacgcc gaagcgctgc
     1861 aaaatccgtt gcagttcgga tccgttggcc tcgctcacgc aatcatcgcc gatccagaca
     1921 cggccggact ccggagtcag caggcgcacc atgtggcgca ggctcacgct cttgccggtg
     1981 ccggagagcc ccacaatcac gaacgtctcg ccgcgttgaa tctcgaagct caaatcatcg
     2041 agcaccgttt tgcggccaaa gcgcttggtg acgttttcaa agcggatcat cggtaggcca
     2101 gcctggtgac gaaatagccc agtgtcagaa tcaacaggaa cgacacgacc accgtgcgcc
     2161 gcgtggcccg ccccaccccc accgcgccct cgcctgtgat gaagccctga tagcaggaca
     2221 cacacaccac aatcaccccg aagatgaacg ccttcagcag cccgacatac aaatccttgt
     2281 tatgcgtgta gcggaacgcg ccatcgaaat aggattcata ggacacaccc agcatggtgg
     2341 cccccaccac ggagccgccg aacaggccga tcatgtccat atagagcgcc agcagcggcg
     2401 tcatggccgc caatgcgacc aagcgcggca tcatcagata gcgcagcggg tcaatgccca
     2461 tcacatccag cgcagcgatt tcctcggaca ccgtcatggt gccgatttgc gcggcaatgg
     2521 ccgaacccac gctggccgcc accacgaggc cggtcatgaa cggacccatt tcgcgcagca
     2581 tggcgttgca caccagcgtg ccgatggtcc actcctgccc gaacgtccgt agcgccaagc
     2641 ccagttgcag cgccaaaatc atgccggtaa acaggccaac cacggacacc acgccaaggc
     2701 tcttgatgcc caccagatag cactgctgca tcacgtccgc ccggttgcgc cgcgaaaaca
     2761 tgaagggcag atatttcacc gcgcccagca gcatcattag cgcgcgcccg ctaccctggc
     2821 acgacgcaat cacccggcgg cccaacgcgg cgatggcgtt gaccggcggc tccagatagg
     2881 gtggttcgcg ggtaatcatg gttcaagttc ggccgcggcc gcggtgggcg ccggcttcaa
     2941 gggaatccgg cccagccaca gcacgcgcac ctgacgattt gtagcggttc ggtcgtacgc
     3001 gagcaaccct ttcagcaccg accagcgtcg cgcctcgttg cgtgccgcgc gctcgtgccg
     3061 ccaaagcggg aacagcgaaa acgcccgcgc ctcctccgcg cggctggttt ggcggtacag
     3121 cccccacagc acatcgagat cgcgggagtc ctgttgcgcg cggtaatccc acagtgtcca
     3181 gagcggcgcc cagctccgtt ccaccgccgg cggctgcccg gcgggccaca ggtccagcca
     3241 gcgccagcgc agcgtttgcg catccacatc ggcctgccgc gaaaagagcg gcca
//
LOCUS       DAOPNP010000160         3278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_31766_length_3278_cov_7.000621, whole genome shotgun sequence.
ACCESSION   DAOPNP010000160 DAOPNP010000000
VERSION     DAOPNP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31766_length_3278_cov_7.000621"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PKI64_10430"
     CDS             complement(<1..963)
                     /locus_tag="PKI64_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE01452.1"
                     /translation="MKFEFTAPARVIFGPGTLTQAGALAVSLGRRALVVTGRHPARAQ
                     RLLALLQEHGVATESFAVSGEPTTDVVALGAAAARRFRAELVVGFGGGSPLDAAKAIA
                     AVAANGGEPLDYMEVVGRGQPLVKPPLPLLAIPTTAGTGSEVTRNAVLTSLAHGVKAS
                     MRSVSMVPRVALVDPELTHDLPPAVTASTGMDALTQLIEPLTCNKTNPLVDALCREGI
                     GRVARSLHRAFAEGHHAAAREDMALASLFGGFALANAGLGAVHGFSGVIGGMYRAPHG
                     AVCAALLPHVMAANHRALQARVPESPLAVRYDEVARLLTGNPAAR"
     gene            complement(970..1275)
                     /locus_tag="PKI64_10435"
     CDS             complement(970..1275)
                     /locus_tag="PKI64_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOE01453.1"
                     /translation="MVIVLVDVHVKPEDVPAFREATAENARNSVQEPGIARFDVVQRG
                     DDPTRFLLYEVYRSEEAIAVHKVTAHYAAWRDAVAPMMAQPRTMAKYVNVFPADADW"
     gene            1765..>3278
                     /locus_tag="PKI64_10440"
     CDS             1765..>3278
                     /locus_tag="PKI64_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE01454.1"
                     /translation="MNAGDNSALGKYLSEVRRLYRLGEATEHSYRGHLQNLFADIFPA
                     LRLVNEPARIECGAPDLALLRRDLVIGYIEAKDIGKDLGREEKSAQMSRYLPALDNLI
                     LTDYLEFRFYRSGKPTQSIRVAEIQGGKFVGRPENHETFLRLIGDFAQWKGVQIRSAA
                     VLAERLAKKARLMRDIFRQVLAKDPTPDNPLQEQLAAFKQILLHDLDAETFADIYAQT
                     IAYGMFAARLHDPTPDDFSRQEAAALVPKSNPFLRNLFGSIAAVDLDDRVAWVVDDLV
                     EIFRTVDLKDILADFGKATAQEDPFMHFYETFLAAYDPALRKGCGVYYTPQPVVRFIV
                     RAVDQILKTSFGLPAGLADNATVVKSVPVEGDSARGGRDKFIERTFHKVQLLDPACGT
                     GTFLAEVVRHIRDAFAGQEGLWPNYVEKDLIPRINGFEILMASYAICHLKLELLLRQT
                     GYDPQGLARRLNVFLTNALEEADPATGTLFASWLSREAREANRVKKETPVMVVIG"
BASE COUNT          624 a         1168 c          890 g          596 t
ORIGIN      
        1 gcgcgccgcg gggttgccgg tcagcagtcg cgccacctcg tcatagcgga cggccagcgg
       61 gctctccggt acgcgggcct gcaatgcgcg atggttggcc gccatgacgt gcggcaacag
      121 tgccgcgcaa accgcgccgt gcggcgcgcg gtacatcccg ccaatcactc ccgaaaaacc
      181 gtgcactgcc cccagccccg cgttggccaa cgcaaaaccg ccgaacaggc tggccagcgc
      241 catgtcctcg cgggcggcgg catgatgccc ctccgcaaac gcgcggtgca acgaccgcgc
      301 cacgcggccg attccttccc ggcacaacgc atccaccagc ggattggtct tgttgcaggt
      361 caacggctcg attaattgcg tcagtgcgtc catgccggtg ctggcagtca ccgccggcgg
      421 caaatcatgg gtcaattcgg ggtccacgag ggccacgcgc ggcaccatgc tgacgctgcg
      481 catactggcc ttgaccccgt gcgccagcga ggttagcacc gcgttgcgcg tcacttccga
      541 ccccgtgccc gccgtggtgg ggatggccag caagggcagc ggcggcttga ccagcggctg
      601 accgcggccg accacttcca tatagtccag cggctcgccc ccgttggcgg ccactgccgc
      661 gatggccttg gcggcatcaa gcggactacc cccgccgaac cccaccacca actccgcgcg
      721 gaaacgtcgc gccgccgccg cgcccagcgc cacgacatcc gtggtaggct cgccgctgac
      781 ggcaaacgac tccgtcgcca cgccatgttc ctgcaacagc gccagcagtc gctgcgcgcg
      841 cgccgggtgc cggcccgtca ccaccagcgc ccgccgcccg agactgacgg ccaacgcgcc
      901 ggcctgcgtc aacgtgcccg gcccaaaaat cacccgcgcc ggagcggtga actcaaactt
      961 catgacgccc taccaatcgg catcggccgg aaacacattc acatatttcg ccatggtgcg
     1021 cggctgcgcc atcatgggcg ccacagcatc gcgccacgcc gcgtagtgcg cggtgacctt
     1081 gtgcaccgcg atggcctcct ccgaccggta cacctcatac agcaggaaac gcgtgggatc
     1141 atccccgcgc tgcaccacgt cgaaccgcgc aattcccggc tcttgcacgc tattgcgcgc
     1201 gttttccgcc gtggcttccc gaaacgccgg aacatcttcc ggcttcacgt gcacatccac
     1261 caatacaatc accattgtgc gcctccttgt ttcaacgcca tcgtattcca cccccccgcc
     1321 ccctgtccag acccctgcat gatcggcttt ctatcatgtg ccgcccctga tatccgccag
     1381 cggtcggcgg ccagcggttc tcgccttcaa agctctgctc aatctggcag aaggacagca
     1441 ggctttttga ttccaccaga atcacagcaa accgttttgt tgctattgta gcatcatttt
     1501 aatagcaaca aatagctttg ttgctaacct atcgaaccca agcaaattcc ttcatttgct
     1561 caccttccaa ccactcaatg agattgcaca acatcttatt ccacaataag ttggattcta
     1621 ttcatagtta gataaacact tttccggagc acagacgaaa ccgcaagtcg gcagtgtaac
     1681 gcccccctgc cagacccccg cgcggatttt gggcccgcga ccgcgggttg acgggctgac
     1741 gccgtccagc gtaggatggc gcccatgaat gctggcgata actcagcgtt ggggaaatac
     1801 ttgagcgaag tccggcggct gtaccggctg ggcgaggcca ccgagcattc ctaccggggc
     1861 catctccaga atctctttgc cgacattttc ccggccctgc gtctggtcaa cgaacccgcc
     1921 cggattgaat gcggcgcacc ggatttggcc ctgctgcggc gcgacttggt catcggctat
     1981 atcgaggcca aggacatcgg caaggacttg gggcgcgagg aaaagtccgc ccagatgagc
     2041 cgctaccttc ccgccctcga caatctgatc ctgaccgact acctggaatt ccggttctac
     2101 cgctccggca agccgacgca atccattcgg gtggccgaaa tccaaggagg aaagttcgtg
     2161 gggcgtcccg agaaccacga gacgtttctg cggctcatcg gcgactttgc gcaatggaag
     2221 ggcgtgcaaa tccgttccgc cgcggtgctg gccgaacgcc tggccaagaa agcccggctg
     2281 atgcgggata ttttccgtca ggtactcgcc aaggacccca cgccagacaa cccgctgcaa
     2341 gagcaactgg ccgccttcaa gcagattctg ctgcatgatc tcgatgcgga aaccttcgcg
     2401 gatatttacg cccaaaccat cgcttacggc atgttcgcag cccgcctcca cgaccccacc
     2461 cccgacgact tttcccgcca ggaagccgcc gcccttgtgc ccaagtccaa tcccttcctc
     2521 cgcaatctgt ttggttcgat tgccgcggtg gacctcgacg accgcgtcgc gtgggtggtg
     2581 gatgacctcg tggaaatctt ccgcaccgtg gacctcaagg acatcctggc cgatttcggc
     2641 aaggccaccg cgcaagaaga ccccttcatg cacttctatg aaaccttcct cgccgcctat
     2701 gatccggcct tgcgcaaagg ctgcggggtc tattacacgc cccagcccgt cgttcggttt
     2761 atcgtgcgcg ccgttgacca gattctcaaa accagttttg ggctgcccgc gggccttgcc
     2821 gacaacgcca cggtggtcaa atcggtgccg gtcgaaggcg attccgcccg cggcggccgg
     2881 gacaaattca tcgagcggac cttccacaag gtccaactcc tcgacccggc ctgtggcact
     2941 ggcacctttc tggcggaggt cgtccgccat attcgggacg ctttcgccgg ccaggagggc
     3001 ctctggccga attatgtcga gaaagacctg atcccccgca tcaatggctt tgagattctg
     3061 atggcctcct atgccatctg ccacctcaaa ctcgaactac tcctccggca aaccggctac
     3121 gatccccaag gcctcgcgcg acgcctcaac gtcttcctca ccaacgcgct cgaagaagcc
     3181 gaccccgcca ccggcaccct ctttgcctcc tggctttccc gcgaggcccg cgaagccaac
     3241 cgcgtgaaaa aagaaacccc cgtcatggtc gtcatcgg
//
LOCUS       DAOPNP010000161         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_33174_length_3173_cov_8.213598, whole genome shotgun sequence.
ACCESSION   DAOPNP010000161 DAOPNP010000000
VERSION     DAOPNP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33174_length_3173_cov_8.213598"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1911
                     /locus_tag="PKI64_10445"
     CDS             160..1911
                     /locus_tag="PKI64_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE01455.1"
                     /translation="MTVAFSPRGEALHLILNELRTATSRVDLAQFYLTHPDLIDALCA
                     LPAKGIPVRLLTDVTMGEAAQQPTLDKLTRHGVQLFLIEPPKRGKMHMKNLVVDGQTV
                     ITGTANWTQQAFGLNCEDTIKIASPALAAVYLAKMDELTASDMAAEIHGSGAKPERLS
                     FPLPKPPAVRSPPGRVNAPGTRRFSAEAPESFFLPNPGPFEHLAEQIRSATSRVDVAI
                     FLLNEDRIVDALTERAQVGDCAIRILADVGMLGSGNLEVLQNLAAAGVEIRTFGSDRE
                     SLHMKTGIIDGHFFWTGSANWTKGAMTLNIEDMLCFDSPELAEWYSNWLDEFAKICQP
                     FVPLQTQTTFVETNAVPTGKWPIGLPPTAPRTDWDHLVEHVDFPPLETNAWVAYLPDE
                     AYAPVLLDLIRNAHQTILIAMYKLAEPGEKVATGFLGQIIAELEKATARGVYVSLLLH
                     VPVSPQDALYDAHSRWAERLRAKGIDVRLGLPALPMHEKFVSVDLCKIIVGSHNWSAG
                     ALDDSRVYESSALVIYPQPQKWLADYFFSRPMVSHMASREAWERELTLIRHTKNMNTS
                     DKYQLLESFGVEVGPAP"
     gene            2061..2858
                     /locus_tag="PKI64_10450"
     CDS             2061..2858
                     /locus_tag="PKI64_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOE01456.1"
                     /translation="MRPPTVARGHAGSCFVVLGALLLLTAISQADEISLRNGTSLTGR
                     VLMVQSSSYVFQDNASHGAPQNIPKTAVRYVLYDDPAMADRAFGIRSALRHAEGEASL
                     VRLLPTRPFGQAILEAVQNAQTSIWVSAYYISGSSTSPIKDFYTVLEDKARQGLEVIV
                     ISEYGPGTGARVREATYNFARTLEQSGVRVLFMSGYRILHKKMILVDNQNAFLGSANL
                     TMAGTLQSDEMNVHIHQPAFVARMRKDFEKIMASAKYAEVLKPMESK"
     gene            2855..3124
                     /locus_tag="PKI64_10455"
     CDS             2855..3124
                     /locus_tag="PKI64_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01457.1"
                     /translation="MSINRHPARWAILMPIMCSAWLFIALPAYPAGKSPDDCKLENCE
                     EWDEYANDCIDLPKNCFILAWFGEDRDDGDCNQDIIYNTRGVPIS"
BASE COUNT          704 a         1021 c          809 g          639 t
ORIGIN      
        1 cgccagccgc cagccgccag ccgccaaccg ccagacgcca gccgccagcc gccagacgcc
       61 agacgccaga cgcgtaaaca ctggcgtttt attttttctg gtttcctagt tccgttgtgc
      121 tttctcttgt cgggaatctt cccggcgcag gcggttgaag tgactgttgc cttttccccg
      181 cgcggcgagg cactccatct gatcctgaat gaattgcgca ccgccaccag ccgcgtggac
      241 ctcgcccagt tctatctcac acatcccgat cttattgatg cgctttgcgc attgccggcc
      301 aaaggcatcc ccgtgcgctt gttgacggat gtcaccatgg gcgaagccgc ccaacaaccc
      361 actctggata aactaacccg ccatggtgtc cagttatttc tgatcgaacc gcccaagcgc
      421 ggcaaaatgc acatgaaaaa cctcgtggtg gacggccaaa ccgtcatcac gggcaccgcc
      481 aactggactc aacaggcctt cggcctaaat tgcgaagaca ccatcaaaat cgcctcgccc
      541 gcgctggcgg cggtctattt ggccaagatg gatgaattga cggcctccga catggccgcg
      601 gaaattcatg gctccggtgc gaagcccgaa cgcctatcct tccccctgcc caagcccccg
      661 gccgttcgct cgcctccggg tcgggtgaat gcgcccggca cccgaagatt ttcggcggag
      721 gcgcccgaaa gctttttcct gcccaatccc ggaccattcg aacacctggc cgagcaaatc
      781 cgctccgcca ccagccgcgt ggatgtcgcc atcttcctct tgaatgaaga ccgcatcgtg
      841 gacgcgttaa ccgaacgtgc ccaagtcgga gactgcgcca tccgaatctt ggccgatgtc
      901 gggatgctgg gttcaggaaa tctggaagtc ctgcaaaatc tggcggccgc cggtgtcgaa
      961 attcgcacgt tcggttcgga ccgcgaaagc ctgcacatga aaaccggcat tatcgatggc
     1021 cacttctttt ggacgggttc cgccaactgg accaagggcg caatgacctt gaacatcgaa
     1081 gacatgctct gcttcgactc tcccgagttg gccgagtggt acagcaactg gctggatgaa
     1141 ttcgccaaga tatgccaacc gtttgtcccg ctacagacgc agacaacatt cgttgagacc
     1201 aacgccgtac cgaccggaaa atggccgatc ggcctgccac ccacggcgcc tcgcaccgat
     1261 tgggatcatc tggtggaaca cgtggatttc ccgccgttgg aaaccaatgc ctgggtcgcc
     1321 tacctgccgg acgaagccta cgcccccgtg ctacttgacc tgatccgcaa tgcccaccaa
     1381 accatcctga tcgccatgta taaacttgcc gaaccgggcg aaaaagtcgc aacaggtttt
     1441 ctggggcaaa tcatcgctga actcgaaaaa gccacggccc gcggcgttta tgtcagcctc
     1501 ttgctccatg tgcccgtgtc accccaagat gccctgtacg acgctcactc ccgatgggcc
     1561 gagcgcctcc gtgccaaggg cattgacgtc cgcctcggac ttcccgccct gccgatgcac
     1621 gaaaagttcg tgtcggttga cctgtgtaag atcatcgtcg gctcgcacaa ctggtccgca
     1681 ggcgcactcg atgactcccg ggtctatgag tccagcgcac tcgtcattta tccgcaaccg
     1741 caaaaatggt tggccgacta cttcttcagt cgaccgatgg tcagccatat ggccagtcgt
     1801 gaagcctggg aacgcgaact caccctcatc cgccatacca agaacatgaa tacttcggac
     1861 aaatatcaat tgcttgaatc gtttggcgta gaagtggggc ccgcgccgtg atctccacca
     1921 tggctcaaat gagaaaagct caagaaaaca agacaatccc gccgagacgg cggggctata
     1981 cagaaagaac acctcccgtc ttcatcgggc ttttcgatcg ccccaaagga catgggctgt
     2041 tgaacattgg aaattggata ttgagacccc caaccgtggc acgagggcat gccggctcct
     2101 gttttgtcgt gctgggtgcg cttcttctcc tgaccgccat ttcccaagcc gatgaaatct
     2161 cgcttcgcaa cggcaccagc ctgactggcc gggttctcat ggtgcaatcc agcagctatg
     2221 tgttccaaga caatgcctcc cacggtgcgc cgcaaaacat ccccaagacc gccgttcgct
     2281 atgttctata cgacgatcct gccatggccg accgcgcttt cggaatccgt agcgccttgc
     2341 gccacgctga aggggaggcc agcttggtgc gcctgctccc cacccgccct ttcggccaag
     2401 ccatcctcga ggctgtccag aacgcccaga cttccatctg ggtcagcgcc tactacatct
     2461 ccggcagttc caccagccct atcaaggatt tttacaccgt cttggaggat aaggcgcggc
     2521 aaggcctcga ggtaatcgtc atcagcgaat acggccccgg caccggcgcc cgcgtccgcg
     2581 aggccaccta caacttcgcc cgcacgctgg agcaatccgg cgtccgcgtc ctgttcatga
     2641 gtggctaccg catcctgcac aagaagatga tcctggtgga taatcaaaac gcatttctcg
     2701 gcagcgccaa cctcaccatg gccggcaccc tccaaagcga cgaaatgaac gtccatatcc
     2761 accaacccgc cttcgtcgcc cgaatgcgca aggatttcga aaagatcatg gcctccgcca
     2821 aatatgccga agtcctcaag ccaatggaga gcaaatgagt ataaaccgac atcccgcccg
     2881 atgggccatc ctgatgccga tcatgtgcag cgcttggctg ttcattgcct tgcccgcata
     2941 tccggcagga aaatcacctg acgattgcaa gcttgaaaac tgcgaagaat gggacgaata
     3001 cgccaacgat tgcatcgatt tgcctaaaaa ttgttttatt ttggcttggt tcggagaaga
     3061 ccgtgatgat ggagattgca atcaggatat catctacaac acccgggggg tcccgatatc
     3121 ctgagttcga cacctctgaa atggaaagtc ataactcctt gggacacagg acg
//
LOCUS       DAOPNP010000162         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_33383_length_3159_cov_2.922680, whole genome shotgun sequence.
ACCESSION   DAOPNP010000162 DAOPNP010000000
VERSION     DAOPNP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33383_length_3159_cov_2.922680"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /locus_tag="PKI64_10460"
     CDS             <1..1612
                     /locus_tag="PKI64_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE01458.1"
                     /translation="EAIEGLRISNHSYNERVGWDYIEGEWNWFGDPDISGTEDFKFGL
                     YMTYDFYLDEALYHFQPLLSVRSAGNKYANYGPGTSVDHWEFHEVEGSKIDVWTNTYR
                     PNNGTNGYDLLPPTACAKNQLVVGAIYKNTNGYVTTNDIILADFSAVGPTDDGRIKPD
                     IVAPGVEISTTKNTSFSAYSTNENGTSFAAPAVAGSLNLIRKQHEDIFGTNQMILSSS
                     LRALAIHTATPATTNGAPDYRHGWGAFNAEACAELVKYNEKDVPTPEALGIPPSSIET
                     NMEIYVISPILYASSNSIESYYDSPDGPMIRSVAVNYNLGTNETRAGLQYVKDFLLPE
                     GEEINFPVTPLGGEPLMVTIAWSDYPGTPPSAIVNPTNLMLVNDLDLRLISPSGTTNF
                     PWVLDPANPSAPATKGDNFRDNVEKVLIENPEAGEYTVMVTHKGELEQGHQFVSMVLS
                     GHQPDEWSAPGLYNVTPYPIDNMMTVGWNAIPGFSYYIMARTNMIFGNWNSLAVVNAL
                     TDQMEVTIPLYTNTLYFTLGGGQSGVYQ"
     gene            1609..2343
                     /locus_tag="PKI64_10465"
     CDS             1609..2343
                     /locus_tag="PKI64_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE01459.1"
                     /translation="MKTSLLGLLLTAGALFAPVNLWAYMAMSFGSDEVVYVEAEGWYN
                     DFSDILLPKSMDLDGDGVWDAGVQNSMMSYGAPGDGFMGAAIATQPSVWVYGFAETTG
                     SHETGDLFTRGCASPFAAGEVLPRVEDDPTRWWGNNPATPDNSIIICATEQYGGNVSH
                     YNTGLNASYYRSRYYLGLALEKPEGFYYAWVEVFHGGRALIQVQNYAYETEPNKTLVF
                     GAIPEPSTIALLAAGGLLLALRRKHK"
BASE COUNT          765 a          780 c          883 g          731 t
ORIGIN      
        1 ggaagccatc gaaggccttc gcatttccaa ccattcctat aacgagcggg tgggatggga
       61 ctatattgag ggcgaatgga actggttcgg ggatcctgac atttccggga cagaggactt
      121 taaatttggc ctctatatga catatgactt ttatttagac gaagcgcttt accattttca
      181 gccgcttttg tcagtgcgtt ccgcgggcaa caaatatgct aattacggac cgggcacttc
      241 tgttgatcac tgggaatttc atgaggttga aggttcaaag atagatgtct ggaccaatac
      301 gtatcggccg aacaacggta cgaatggcta tgaccttttg cccccgacag cctgcgcaaa
      361 aaaccaactg gttgtgggcg caatctataa aaacacaaac ggatacgtca caacaaatga
      421 catcatactt gctgatttca gtgcagtagg ccccacggat gacgggcgga ttaagcccga
      481 tatcgtcgct ccaggcgtgg agatttcaac caccaaaaac acttcgtttt cagcctattc
      541 gaccaatgag aacggcacca gtttcgccgc ccctgccgtt gccggttccc tgaatctcat
      601 caggaagcaa cacgaggata tattcggaac aaaccagatg attctttcgt cctcgctccg
      661 ggccttagcc attcacaccg caacgccggc aaccacaaat ggagccccgg attacaggca
      721 cggatggggt gcattcaatg cagaagcctg tgccgagctg gtgaagtaca acgaaaagga
      781 cgtgccaacg ccagaagctt taggcattcc cccttcgagt attgagacaa atatggagat
      841 atatgttatt tccccgattc tctatgccag ctcaaacagt atcgaaagtt attatgattc
      901 gcctgatggg ccaatgatca ggtctgttgc ggtcaattat aatttgggca caaacgaaac
      961 gcgcgctggt ctccaatatg tcaaagactt ccttttgcct gaaggcgagg aaatcaactt
     1021 ccccgtgact ccattgggag gagaaccgct tatggtgacc attgcctggt ccgattatcc
     1081 gggcaccccg cccagtgcca tcgtgaatcc aacaaatctg atgcttgtga acgatttgga
     1141 tttgcgtctg atttccccgt cagggacgac caacttccct tgggtgcttg atccggcgaa
     1201 tccatctgca cccgcgacga aaggggataa tttccgagat aatgtcgaaa aagtgctgat
     1261 agaaaaccct gaagccggag agtacacagt aatggtaact cataagggcg aattggaaca
     1321 ggggcaccaa tttgtttcaa tggttctttc cggccaccaa ccagatgaat ggagcgctcc
     1381 gggactgtac aatgttacac cctatccgat tgataatatg atgacggttg gctggaatgc
     1441 aattccagga ttttcctact acatcatggc ccggacaaat atgattttcg gaaactggaa
     1501 ttcattagct gttgtcaatg cgttaacgga tcaaatggaa gtcacgattc ccctatatac
     1561 caatactctg tattttacac tgggcggcgg gcagtctggg gtttaccagt gaaaacatcc
     1621 ttgcttgggc tgttgcttac ggcaggcgca ttgtttgcgc ctgtcaacct ttgggcctat
     1681 atggcaatgt ctttcggttc ggacgaagtt gtttatgtag aggcggaagg atggtataat
     1741 gatttcagtg atatactcct tcccaagagc atggatttgg atggcgatgg tgtttgggat
     1801 gccggagttc aaaattcgat gatgagttac ggagcgccgg gcgacggatt catgggggct
     1861 gccattgcaa cgcaaccgag tgtgtgggtg tatggttttg ccgaaacaac tggtagtcac
     1921 gaaaccggcg acctgtttac acggggatgt gcctcgccgt ttgcggcggg agaagtactc
     1981 ccccgtgtgg aggatgatcc gacacgctgg tggggaaaca accctgcaac accagataac
     2041 agcattataa tttgtgccac ggaacaatat gggggcaacg tgagtcacta taacacggga
     2101 ctaaatgcct cctattatcg cagccggtat tatcttggtc ttgcgctgga gaaaccagag
     2161 ggcttttatt atgcttgggt ggaggttttc catggcgggc gcgcattaat tcaagtccaa
     2221 aattatgcct atgagacaga gccgaacaag acgctggttt tcggggcgat tcccgaacct
     2281 tccaccatcg ccctgctggc cgcaggcggc ctgctgctgg ccctgcgtcg caaacacaag
     2341 taaaggcata ttcccaacct gtttttatat attcagtacc gtaagatttt cttacatcta
     2401 tagttttcca tagtatggaa aacttttttg cgatttttcc atgccatgga aaaaatgttt
     2461 ccacagcatg aaaagttgga tttagaggct gttttccggg ggctgtagag cggaacacgc
     2521 ggcgcccctt gtaagccgta acgcgcgacc gaccgaggga ccgggaaaaa cggtgcccgc
     2581 tgcaagcggg cagcacactc aggcgcaccg tgcagcgggt gcgccgtagt gggcgaccgt
     2641 ttttccggtc acgaggtcgg ggatttctcg cgcgttcagg cgaaacaggg gcgttgcggg
     2701 tggagcgaag atttactccg gggggattta ataaatgtcc gaggattgag agaccatacc
     2761 ttggagggcc gaaggcccga ccccagcccc gaacgcgctt gcgcggtcgg ggcggtgcca
     2821 ggcgcggtca ggagtccgcg actggcaggc aggccgccca aaaagcaagt ggccccgtga
     2881 tttttgcgca gcaaaaaggc tcggggcgag ttgcgggcgg cctgggagcg cctttggagg
     2941 ctggttttgg gccaaaagga agagcgtggg cctcccatag cccaaagcga acgattttgg
     3001 gccaaaatcg gccttttaag gcgcgtttta agcgaagggc gcagcccgaa gcgcggatcg
     3061 gaggcggggt tgggctcgcg gcaatctgcc gcgtaccgca ttcacgcgcg ccccgacccg
     3121 aagcccgccg gaggcgggcg caagagcgcg gcagagtgc
//
LOCUS       DAOPNP010000163         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_33881_length_3124_cov_7.837406, whole genome shotgun sequence.
ACCESSION   DAOPNP010000163 DAOPNP010000000
VERSION     DAOPNP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33881_length_3124_cov_7.837406"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..2031
                     /locus_tag="PKI64_10470"
     CDS             214..2031
                     /locus_tag="PKI64_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOE01460.1"
                     /translation="MKQAATNRSSAHPPAARRAGWLLAGWLLASALPAGATQVTFDVQ
                     PRLLNLGESAQATLTFHGVRSAPNVEFPPIAGLQMTGTGQQMQFGTGGSRVTLTYNLF
                     AQKAGSYTIGPYHLDFNGEQIDIPAVTIEVRARAGGNADSPQEMLFARLILPEQQPFI
                     HQMFDVVLAIYSLPGVELTRDVNLLGGFPESGFVLSNFEELQMIREEVSGQIYNLRRF
                     RARARALTAGTFHLEPALRVGVVNPQDRRRNDPFGGFFDPFGGPSATPVSVNPPPADI
                     VVRPLPTADRPADFSGAVGQYEFSVDVRPRELHVGQPITVTLRVQGTGNIAAALPPSF
                     QETDSFKAYEARQVGDTPAPAAESGSKTFEQVVIPRTESLTELPALRFSFFDPVAAQY
                     RAQTVGPFPLTVHPSESGANALLLQVPGTANGSGNTLVLGTDIVYLKSAPARWMRTDA
                     RGISRPAWLGLHAAAPLALAGLFFATRRRNRLATDIALARRQKAPRSARANLRKAEAA
                     LKAGGAPVDVFGALAAAAADYFGHRLNLPPGAVDAPLMLAKLQAANAEAGDQEKWQEF
                     FALSDQVRYASAAAMGRAELSEWIATVSGLLRRAERLRL"
     gene            2028..2807
                     /locus_tag="PKI64_10475"
     CDS             2028..2807
                     /locus_tag="PKI64_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01461.1"
                     /translation="MIARGAQLKRWRWSCAVALVLLALGLPARAANGDDSHRYARQFA
                     DAARAYDDNLLAEAIAGWQALVDEGQALPAVLYNLGNAHYRSGHLGPAILAYRRAQRL
                     LPRDPDIRANLGFAAQSAGIALPQRTPLMALLLDLSRAEWRGCAEVAFWLLFAAGAAW
                     IVWPRGRFISRPACFVLAVALAIAGAGLWAHHDLRATPECVVMQPEQKILASPLATAT
                     PLVAIPEGALVRQVSQRGAWTEVRLDSTRGWLPSDVLAPVP"
BASE COUNT          504 a         1035 c         1024 g          561 t
ORIGIN      
        1 gccaaaaccc gcggaaaaat catccgacac catgacgccc gaagaagccg ccatgcttct
       61 cgatgccatg aaggcgcagg agcagcagca acgtgatcag ttgcatcctt tcctcggccg
      121 ccctgtgccg gtggaaaagg actggtgaac ccgccgcgcc agaagatggg attggcggct
      181 cgcgcggctt cggctagact gccatcccga ttcatgaaac aggccgccac caaccgtagc
      241 tccgcccacc ccccggccgc ccgccgggcc ggctggttgc tggccggctg gctgctcgcg
      301 tcggcgctac cggcgggtgc cacgcaagtg acgtttgacg tccagccgcg cctgctcaat
      361 ctgggcgaga gcgcccaggc caccctcacc ttccacggcg tgcgttccgc gcccaacgtg
      421 gaatttccgc ccatcgccgg cctgcaaatg acgggcaccg gacagcaaat gcagttcggc
      481 acgggtggct cgcgcgtgac gctcacctat aacctgttcg cgcaaaaagc gggttcctac
      541 accattgggc cgtatcatct ggatttcaat ggcgagcaga ttgacatccc cgccgtgacc
      601 atcgaagtac gcgctcgcgc gggcggcaac gccgactcgc cgcaggaaat gctgtttgcc
      661 cgcctgatcc tgcccgagca acagcccttc attcaccaga tgttcgatgt ggtgctggcc
      721 atctattccc tgccgggcgt cgagctgacg cgcgatgtca acctgcttgg cggcttccct
      781 gaatccggtt ttgtgttgag caactttgaa gagctgcaaa tgatccgcga agaagtcagt
      841 gggcaaatct ataacctgcg ccgtttccgc gcgcgggcgc gggcgctgac tgctggcacg
      901 ttccacttgg aacccgcgct gcgcgtgggc gtggtgaatc cacaggaccg ccggcgcaat
      961 gatccgttcg gcggcttctt tgatccgttc ggcggtccca gcgccacgcc ggtttccgtg
     1021 aatccgcccc ctgccgatat tgtcgtgcgc ccgctcccca ccgccgaccg cccggccgat
     1081 ttttccggcg cggtggggca atacgaattt tccgtggacg tgcgcccgcg cgagttgcac
     1141 gtgggccagc ccatcaccgt gactctgcgc gtacaaggca cgggcaacat cgccgccgcc
     1201 ctccccccct ccttccagga aacggattcg ttcaaagcct acgaggcgcg gcaagtcggc
     1261 gacacccccg cccccgccgc cgaaagtggc agcaaaactt ttgagcaggt ggtcattccc
     1321 cgcacggaat cgttgacgga actgcccgcg ctgcgtttca gcttttttga tccggtggcg
     1381 gcgcaatatc gcgcgcaaac cgtggggccg ttcccgctga cggtgcatcc atcagaaagc
     1441 ggcgccaacg ccctgctgct gcaagtgccg ggaacagcca acggcagcgg caatacgctc
     1501 gtgctgggca cggacatcgt ctatctgaaa tccgcgcccg cacgctggat gcggactgac
     1561 gcgcgcggga ttagccgccc ggcttggctg gggttgcacg ccgccgcgcc gctggcgttg
     1621 gccgggcttt tcttcgccac gcggcggcgc aatcggcttg cgacggatat cgcgctggcc
     1681 cgccgccaaa aagcgccgcg cagcgcccgt gccaacttgc gcaaggcgga agccgcgctg
     1741 aaagccggtg gcgcacccgt ggacgtgttc ggcgcgctcg cggcggcggc agcagattat
     1801 tttgggcacc ggctcaacct gccgccaggc gcggtggatg cgccgctgat gctcgccaag
     1861 ctgcaagcgg ccaatgccga agccggcgac caagagaagt ggcaggaatt ttttgccctc
     1921 tccgatcaag tgcgctacgc ctccgccgcc gccatgggcc gcgcggaatt gagcgaatgg
     1981 attgccacag tgtccggcct gctgcggcgt gcagaaaggc tccggctatg atcgcgcgcg
     2041 gtgcccaact gaaacggtgg cgctggagtt gtgcggtggc gctggttctg ctggcgctgg
     2101 gcctgcccgc gcgcgccgcc aatggcgatg actcgcaccg ctacgcgcgc cagtttgccg
     2161 atgccgcccg cgcctacgac gacaacctcc ttgcggaggc catcgccggc tggcaggcgc
     2221 tggtggatga gggccaagcc ttgccggccg tgctgtataa cctgggcaat gcccattacc
     2281 gaagcgggca cctcggcccg gccattctgg cctatcggcg ggcgcagcgc ctgctaccgc
     2341 gcgatccgga catccgcgcc aatctgggct ttgccgcgca atcggcgggc atcgccctgc
     2401 cccagcgcac gccgctgatg gcgctgctgc tggacctgag ccgtgccgag tggcggggct
     2461 gcgcggaagt tgccttttgg ctgctgttcg cggcgggcgc ggcgtggatc gtgtggccgc
     2521 gcggacggtt catcagccgc ccggcgtgtt tcgtgctggc cgttgcgctg gcaatagccg
     2581 gcgcgggctt gtgggcgcat catgatttgc gcgccacgcc ggagtgcgtg gtgatgcagc
     2641 cggagcaaaa gattttggcc agcccgctgg ccacggccac gccgctcgtg gcgattccgg
     2701 aaggcgcgct ggtgcgccaa gtgtcgcaac gcggtgcctg gacggaagtg cggcttgatt
     2761 ccacgcgcgg ttggctaccc agcgatgtgc tggcgccggt gccgtaggga tttttgttgg
     2821 gcgggaatgg ttgaggaacg agaccttttc gccgagaacg gctggaaggt ctgcggccgt
     2881 tccagcccga caatccaacc ggctgctttt gtagggcggg aatggttgag gaacgaaacc
     2941 ttcccgccgg gcaaggctgg aaggtctgcg accgttccgg ccctacaatc caaccggctg
     3001 cttttgtagg gcgggaatgg ttgaggaacg agaccttccc gccgggaacg gctggaaggt
     3061 cggcgacctt tccagccctt ctatgggggc cgtcaagtca tggcgatgga tttattgtct
     3121 tact
//
LOCUS       DAOPNP010000164         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_34174_length_3103_cov_4.970144, whole genome shotgun sequence.
ACCESSION   DAOPNP010000164 DAOPNP010000000
VERSION     DAOPNP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34174_length_3103_cov_4.970144"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKI64_10480"
     CDS             complement(<1..1075)
                     /locus_tag="PKI64_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE01462.1"
                     /translation="MKFYKENSEANPEQPPAPTPGPAHDLTLIRNFSIIAHIDHGKTT
                     LSDRLLELTGAVTKREQRDQLLDDMDLERERGITIKAHPVTMKYIARDGRVYEFNFID
                     TPGHVDFSYEVSRSLAACEGALLVVDTTQGVEAQTVAHAFLAAASHIKIIPVLNKTDM
                     PAAESEVVARQIEDMFAIPREECFHISAKTGVGVAEVLEALVERIPPPVSKYPGDLRA
                     LVFDSEYNTFRGGIIYVRVFDGAIRRGDRIRLMGSGKNYEVQEVGIFNPRPQPVDVLQ
                     AGQVGYFLANIKNTAEIMIGDTVTGARTPTEHPLPGFKAMHPMVFTGLYPVDADDYEK
                     LKFSLEKLALNDSSFHYQTESSVA"
     gene            complement(1116..1793)
                     /gene="rpe"
                     /locus_tag="PKI64_10485"
     CDS             complement(1116..1793)
                     /gene="rpe"
                     /locus_tag="PKI64_10485"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOE01463.1"
                     /translation="MKSSSILILPSILSARMGYLAEDCRHAMEAGADGLHVDIMDGHF
                     VPNLTMGPDVLRAVREAVPAAHLSVHLMVMRPDLYAPRFVEAGADTVLIHVEAPCDLA
                     ATLQGIRAAGKRAGLTLCPETPVSALQGYESLLDEVLIMTVHPGFGGQAFLASGLAKI
                     EEVAQLLPAVDISVDGGINAETGAQCAAAGANILLAGSFLFKAANMRDAIAGMRWQVE
                     SARGAGS"
     gene            complement(1840..2235)
                     /locus_tag="PKI64_10490"
     CDS             complement(1840..2235)
                     /locus_tag="PKI64_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOE01464.1"
                     /translation="MEEFGRERVGERAAAKCFENLVVWQKAHQFVLLVYRLTKMFPRE
                     EIYGLTVQFRRAAVSVAANIAEGFRKRGRADKLRFLNISQGSAEECRYYLILAKDLGY
                     QTDPMAVALLDEVGRLLVAYAKGIARSTA"
     gene            complement(2364..>3103)
                     /locus_tag="PKI64_10495"
     CDS             complement(2364..>3103)
                     /locus_tag="PKI64_10495"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOE01465.1"
                     /translation="ALLSRFLSMPDAEEFYRAQDAAKERRRNEEKAVTDGAAVKPRAK
                     RKRARIASGQGILVLISFCLVVGLFSRLGADPGWVKWLQIASIPPVDPFHFWQSLHEV
                     AHGQVWRLFTPVFLHYSLWHLLINLLWLQELGGVLETRLGTRHVLVLMGLLAMVSNLA
                     QYAVVGADQFMGMNGVVYGMLGYYWARQRLDGWNTPVISPVTYGVLLVFLGLGVFGLM
                     GPAANAAHFSGLLAGAGTGWVVSKNGR"
BASE COUNT          708 a         1022 c          761 g          612 t
ORIGIN      
        1 gcgccacgct ggactcggtt tgatagtgga aagagctgtc gttcaaggcc agcttttcga
       61 gtgaaaactt cagcttttcg tagtcatccg catccaccgg ataaagcccg gtgaacacca
      121 tcgggtgcat ggccttgaag cccggcaacg ggtgttccgt cggtgtgcgg gcaccggtca
      181 ccgtgtcgcc aatcatgatt tcggcggtgt ttttaatgtt ggcgagaaaa tagcccacct
      241 gccccgcttg caggacatcc accggctgcg gacgtggatt gaagatgccg acctcctgca
      301 cttcgtaatt tttaccggac cccatcagcc ggatgcgatc cccgcgccgg atggcgccat
      361 caaaaacgcg cacataaata atgccgccac ggaacgtgtt gtattccgag tcaaaaacga
      421 gggcccgcaa gtcgcccgga tacttggata ccggcggagg gatgcgctcc accagcgctt
      481 ccaacacctc cgccaccccc acgccggttt tggcgctgat gtggaaacac tcttcacggg
      541 gaatggcgaa catatcttca atctgccgcg caaccacctc cgattcggcc gccggcatgt
      601 cggttttgtt cagcactggg ataatcttga tgtgcgacgc cgccgcaagg aaggcgtgcg
      661 ccaccgtttg cgcctccacc ccctgcgtgg tgtccaccac caagagcgcc ccttcacacg
      721 ccgccaaact gcgactgact tcataagaaa aatccacgtg ccccggggta tcaatgaaat
      781 tgaactcata cacccgaccg tcgcgcgcga tgtatttcat ggtcaccgga tgcgccttga
      841 tggtgatgcc acgctcgcgt tccaggtcca tgtcgtccag caactggtcg cgctgctcgc
      901 gcttggtcac cgcgccggtc agttccaaca gccggtcgga gagcgtcgtt ttgccgtggt
      961 caatgtgcgc gatgatgctg aaattgcgga tcaacgtcag atcgtgcgcc ggccccggcg
     1021 tgggtgccgg tggttgctcg gggttggctt cggagttttc cttgtaaaac ttcatgatcg
     1081 tcactgaatc acaatgttca tggccggatg gcgtctcaag agccggcgcc ccgcgcggac
     1141 tctacctgcc agcgcatccc ggcaatcgcg tcgcgcatgt tggcggcctt gaacaggaaa
     1201 gaacccgcca acaggatgtt ggcgccggct gccgcgcatt gcgccccagt ttcggcattg
     1261 atgcccccat ccactgaaat atccactgcg ggcaacaact gcgccacttc ctcgatcttg
     1321 gccaggccac tggccagaaa cgcctggccg ccaaaacccg gatgcacggt catgatcagc
     1381 acctcatcca gcagcgattc atagccttgc agggccgata ccggtgtttc cggacacaat
     1441 gtcaagccag cccgtttgcc ggcggcgcga atcccctgca acgtagccgc cagatcacac
     1501 ggcgcttcca catggataag taccgtatct gcgccggctt ctacaaatcg cggcgcatag
     1561 agatccggcc gcatgaccat caaatgtacg ctcaaatggg ccgccggtac ggcctcgcgc
     1621 actgcccgca agacatccgg ccccatggtc agattcggca caaaatgccc atccatgata
     1681 tccacatgca acccatccgc ccccgcctcc atggcgtggc gacagtcttc ggccaaatac
     1741 cccattcggg ccgaaagaat ggagggtagg atgagaatgg aagaagattt cattaggggg
     1801 agtatagaaa taacggagtc gggtagtata gtaggaaaat caggccgtag accgcgcaat
     1861 gcccttggca taggcgacca gcaagcggcc gacctcatct agcaacgcaa ccgccatagg
     1921 gtctgtttgg taacccaaat ctttggccaa gatgagataa taccgacatt cctccgctga
     1981 cccttggctg atgttcagga agcgcaactt gtcggcacgg ccccgcttac ggaaaccctc
     2041 ggcaatattg gcggctacgg ataccgccgc ccgtcggaac tgaactgtta gaccgtatat
     2101 ctcctcgcgt ggaaacattt ttgtcagacg gtagaccaag agaacgaact gatgcgcttt
     2161 ttgccagacc acaagattct caaaacattt tgccgccgcc cgttcaccca ctctctccct
     2221 cccaaactct tccatcccta cactcctcct cgcctactac tcccccctat actcctaaac
     2281 tcccaccctc ctacactcct acactcccac actcctacac tcccacactc ctacactcct
     2341 atactcctaa actcccaccc tccttatctt ccattcttcg ataccaccca tcctgtccct
     2401 gcgcctgcaa gcaaacctga aaaatgggcc gcattggccg ctgggcccat cagaccaaac
     2461 acccccagcc ctaaaaatac cagaagcacc ccgtaggtta cgggtgaaat taccggggta
     2521 ttccacccat ctaagcgctg gcgagcccaa taataaccga gcatgccgta taccacgcca
     2581 ttcatcccca taaactggtc cgccccaact accgcatact gcgccaaatt ggaaaccatc
     2641 gccagcaagc ccatcaacac caaaacatga cgcgtaccca agcgggtttc cagcacaccg
     2701 cccaactcct gcaaccacaa cagattaatt aacaaatgcc agagagagta atgcagaaaa
     2761 acaggcgtaa aaagtcgcca cacctgaccg tgcgccacct catgcaacga ttgccaaaaa
     2821 tggaacgggt caacgggagg gatactggcg atttgcaacc acttcaccca gccggggtcg
     2881 gctcccagcc ggctgaatag ccccaccaca agacaaaagc tgatcaaaac caggatgcct
     2941 tggccactgg caatgcgcgc acgctttcgc ttggcccgcg gtttgacggc cgccccatcg
     3001 gtcacagcct tttcttcgtt gcgccggcgt tcttttgctg cgtcttgcgc ccgatagaac
     3061 tcctccgcgt ctggcatgct gaggaacctg gacagtaagg ccc
//
LOCUS       DAOPNP010000165         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_36787_length_2933_cov_4.828353, whole genome shotgun sequence.
ACCESSION   DAOPNP010000165 DAOPNP010000000
VERSION     DAOPNP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36787_length_2933_cov_4.828353"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..581
                     /locus_tag="PKI64_10500"
     CDS             168..581
                     /locus_tag="PKI64_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOE01466.1"
                     /translation="MKREDQALFTAVLRETLARGQAVAFRVRGPSMLPWLREGQKVMV
                     APLAGRQLRRGDIALFWRNEQEPVLHRVVQPPTADGVVACRGDAEWGPPELVPLAAVA
                     GVVALPLWRRRLFLLIHPARRWVNRLILQWRQRHG"
     gene            574..1704
                     /locus_tag="PKI64_10505"
     CDS             574..1704
                     /locus_tag="PKI64_10505"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE01467.1"
                     /translation="MVEPLQTFADLQRCCAAALHGNLGRVELSPGVRPEDLLAMAERH
                     RVVPLCAAGLAAEQRGAFRRRVLSLAQHMVVLDREVALVADILAGAGGEFLILKGPAL
                     ARQAYPTEEWRVSDDVDLWVPASRAANAAIALRAAGFERSPPLTERVAAAARRAGIEA
                     SFRHPERGRLVEVAHGWASLGPSRRAAREMWNRRVQLNIGGVVVSTLHPVDALLFGCR
                     HGAHHGWDRLSWVMDTAAIWQKLSPAERAEACVVARRRAQTVVLGLGLRLAADLVGIE
                     LSDDAATLASRPRVLALAAGAAVMACPAATERMPMMARLRYERAAQDAWRQKVRMMTG
                     WVFTPTLGDLEGCPLPHALEPAYALLRPLRLLRHPWLKSWFR"
     gene            2148..>2933
                     /locus_tag="PKI64_10510"
     CDS             2148..>2933
                     /locus_tag="PKI64_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOE01468.1"
                     /translation="MKTAETTNGKRTRSGWLLGSLGLLLSLGTLTASAQVSNILAGAP
                     MADSCGLVYFTNTFRNNGPTLNNLFITNVLPSANWAYVPGLSEVTLPSGVVLNGAAAD
                     PTVNGQDLVWDFSSVVTPSDVDHLLITEVFYNYTGPGTKEDHEWFEIYNPTPNPVTLN
                     GWQVGDANPGQLDLLPTFTIAPGEFVIIAGRTNAFLADHPAYTGQVYEVADGKIGSGL
                     NVVPGDGVFLISTMGPVDSVSYGGSTAAFNPAVPGVGAGHSLER"
BASE COUNT          500 a          744 c         1090 g          599 t
ORIGIN      
        1 ttgccgcatg aaaaacggaa caaatctgat tcccggcttt tgtcggtgcc accatgacat
       61 ctgcaaacca gcacaatgcc gtaaatcaac acgccgctgg cgcggtgccg gcgcacggaa
      121 tagccggggc cggaagcaaa aatattaaat tataggactg accccttttg aagagagagg
      181 accaggcgtt gtttacggcg gtgctgcgcg agactttggc gagggggcag gcggtggcat
      241 ttcgggtgcg ggggccgagt atgctgccgt ggttgcgcga ggggcaaaaa gtgatggtgg
      301 ccccgctggc gggccggcag ttgcgccggg gcgatatcgc cctgttctgg cgcaacgaac
      361 aagagcccgt gctgcaccgc gtggtgcaac cgccgacagc ggatggcgtg gtggcatgtc
      421 ggggcgacgc agaatggggg ccaccggaac tggtgccgct ggcggcggtt gccggcgtcg
      481 tggcgctgcc gctttggcga cgccggctct ttttgctgat ccatccggcg cggcgctggg
      541 tgaaccggct cattctgcag tggcggcagc ggcatggttg aaccgctcca gacgttcgct
      601 gatttgcagc ggtgttgcgc ggcggcgttg cacggcaact tgggccgagt ggaattatcg
      661 ccgggggtga ggccggagga tttgctggcc atggcggagc ggcatcgtgt ggtgccactt
      721 tgcgcggcgg gtctggccgc tgagcagcgg ggagcatttc ggcggcgagt gctgtcgttg
      781 gcgcagcaca tggtggtgct ggaccgcgaa gtggcattgg tggcggatat tctggcgggg
      841 gcgggggggg agtttttgat tctgaaaggc ccggcgttgg ctcgacaggc ctatccgacc
      901 gaagaatggc gggtgagtga cgacgtggat ctttgggtgc cggcgtcgcg cgcggcgaac
      961 gccgccatag cgttgcgggc tgcgggattc gagcggtcgc cgccgttgac ggaacgggtg
     1021 gctgcggcgg cgcggcgggc ggggatcgag gcttcgttcc ggcacccgga acgagggcgg
     1081 ttggtggagg tggcgcatgg ttgggcgtca ttggggccca gccgccgggc tgcacgggag
     1141 atgtggaatc gccgcgtgca gttgaatatc ggcggggtgg tcgtgtccac gctgcacccg
     1201 gtggatgcgt tgctgttcgg ctgtcggcac ggcgcgcacc acggatggga tcggctttcg
     1261 tgggtgatgg ataccgcggc catttggcag aagttatcgc cggcggaacg agcggaggcg
     1321 tgtgttgtgg cacggcggcg ggcgcaaacc gtcgtgctgg ggctgggctt gcggttggcg
     1381 gcagacttgg tgggcattga attaagcgac gatgccgcca cactggcgag ccggccccgc
     1441 gtgctggcgt tggcggcggg ggctgcggtg atggcctgcc ccgcggcgac cgaacggatg
     1501 ccgatgatgg cccgcctgcg ctacgagcgc gccgcgcagg atgcatggcg gcagaaggta
     1561 cgaatgatga ccgggtgggt gttcaccccg actttgggcg atttggaggg gtgtccatta
     1621 ccgcatgctt tggagccggc ctacgcgttg ttgcgcccgc tgcggttgct gcggcatcct
     1681 tggctgaaat cctggttccg ctgatcccct ccagcggggg ggggggctga aaaaatccct
     1741 gcggtcaccg cccgcggcta cagcaacagc cacgctcttg gaggcagccg ttataccggc
     1801 ggcgggatgt tggatttcct gtcttattgt agccggccgc agtgaggccg gggagggggg
     1861 gcgtttcgga aagaaaaaca gcggtttcgg aaaaaattag aaaaaaaatg ctgttttcgt
     1921 ttgcatcggg ggggcagatg agtcatcagt tcacccattc accggaagtt tgtttggttc
     1981 ccccaaggct gcctaatcgc acaacacgcc ttgtattttg tggggtcaag cgtttcttcc
     2041 ggcgaaaaga caagtgaagt tttgcgcggt tccaccttgg aaaagcaggg tggaactggg
     2101 cggttttttg gcgttttaaa aaggtgatga ctccgaggag accggatatg aaaacagcag
     2161 aaacgacgaa cggcaagcgg acgcgcagcg gctggctttt gggaagcttg ggcttgttgc
     2221 tgagcttggg cacgctgacg gcgtcggctc aagtgtccaa catactggcc ggggcgccga
     2281 tggcggacag ttgcgggtta gtgtatttca ccaacacttt ccgcaacaac gggcccacgc
     2341 tcaataattt gttcatcacc aatgtgctgc catcggccaa ttgggcctat gtgccgggac
     2401 tgtccgaggt gaccctgcca agcggggtag tgttgaatgg cgcggcggcg gacccgacgg
     2461 tcaacggcca agatctggtc tgggattttt cgtcggtggt gacgccgtcc gatgtggacc
     2521 atctgctgat tacggaagtt ttctataatt atacggggcc ggggaccaaa gaggatcacg
     2581 agtggtttga gatttacaat ccgacgccga atccagtgac gctgaatggc tggcaggtgg
     2641 gcgatgcgaa tccggggcag ttggacctgc tgccgacctt cacgatcgcc ccgggagagt
     2701 ttgtgatcat tgccgggcgg acgaatgcat ttttggcgga ccacccagcc tatacggggc
     2761 aagtttatga ggtggcggat gggaagattg gttccgggtt gaacgtggtc ccgggcgatg
     2821 gggtattcct gattagtact atggggccgg tggactccgt ttcgtatggt ggctccacgg
     2881 cggctttcaa tccggcggtg ccgggcgtgg gtgccgggca ttccttggaa cgc
//
LOCUS       DAOPNP010000166         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_36996_length_2920_cov_9.922513, whole genome shotgun sequence.
ACCESSION   DAOPNP010000166 DAOPNP010000000
VERSION     DAOPNP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36996_length_2920_cov_9.922513"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(604..1536)
                     /locus_tag="PKI64_10515"
     CDS             complement(604..1536)
                     /locus_tag="PKI64_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOE01469.1"
                     /translation="MWEAIAANKRRSAWLIAMLAALLAALGGGLGLLVGGAPESMAYG
                     AFVGLCVWGVLLLINMMSGESVLLSSANAREVSHEDAPQLYNIVEEMRIAAALPAMPR
                     VFIMDTPVPNAFAVGLKPERACVAVTSGLLERLSRDELQGVIAHELGHIANRDTMFMT
                     LAGVTLGAIVLLADMGMRMMFWGGGGRRRSSNDKGGGGAAFAIMAVIAIVLAILAPLI
                     ARLLYFACSRQREYLADASAAQFTRYPAGLAHALQKIAAQQEPKKIQTNRVVAPMYVI
                     NPLAAAGSASIFGTHPPTADRIQRLLAMQGEAPA"
     gene            complement(1576..2133)
                     /locus_tag="PKI64_10520"
     CDS             complement(1576..2133)
                     /locus_tag="PKI64_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOE01470.1"
                     /translation="MAPVLLLLGVLVVLVLWFVSIYNGLVQLRNYVDNAWAQIDVQLK
                     RRHDLIPNLIETVKGYMAHERGTLEAITKARTDAMKAHGPAEAGPAESQLSGALRGLM
                     VNVERYPDLKASQNFLALQEELSSTENKISFARQAYNDSVTRYNNKIQTIPSNLVAGP
                     AGFSKRDLFELKDEAERAVPKVSFA"
     gene            2861..>2920
                     /locus_tag="PKI64_10525"
     rRNA            2861..>2920
                     /locus_tag="PKI64_10525"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          654 a          774 c          924 g          568 t
ORIGIN      
        1 ttgatccaca tcaccatgat gcatgaacga aaccccatag catctacaat ccaaacgggg
       61 gcagttttgg gagttggtgg gggatcatgc cggtgcctcg ccctgcatag ccagtagccc
      121 tgagttccga aatgggacac ccaaccagac gtgctgatat ttcacgaatc gaacggtatt
      181 tttgagaaaa aaagccaaca aaaacagttt gccaagctcg caggggagag caaagcatac
      241 cggggctgca atctatttca tttttgcgga cgggacagag cccattccgc cacattgcgg
      301 cgccgtggag ggttggcctc cgtgccgccc acccattttt atcagttttg aaaatttgcc
      361 tcgtgatcga atgagaggaa ccggaatttt ggaaagtatt tttcaaatcg atcagcgtct
      421 tttggggaaa actaaccatc tgaatccgga ttttcggcat atttccagtg gaaaacattc
      481 actattttgg aggcgggaca cccaaaaacg aaatcgtact gttgcaatgc aacgagttac
      541 gacgagattt tcggggtgct cggaactcag gaaacggggg cggttttgtg agttgatggg
      601 ggatcatgcc ggtgcctcgc cctgcatagc cagtagccgc tggatgcggt cggccgtggg
      661 cgggtgggtg ccgaagatgc tggccgagcc ggcggctgcc aacggattga tcacatacat
      721 gggcgccaca acacggttgg tttggatttt tttcggctcc tgctgcgccg caattttttg
      781 gagcgcgtgg gcgaggccgg cgggatagcg ggtgaattgc gcggcggagg catcggccag
      841 atattcgcgc tggcgcgagc aggcaaagta aagcagccgc gcaatcagcg gcgcgagaat
      901 cgccaggacg atggcgatga cggccatgat cgcgaaggcc gcgccgcccc cgcctttatc
      961 attggatgaa cgccggcgtc cgccgccgcc ccagaacatc atgcgcatgc ccatgtcggc
     1021 cagcagcaca atcgcgccga gcgtcacgcc ggcgagggtc atgaacatgg tgtcgcggtt
     1081 ggcaatgtgg cccagctcgt gggcgatcac gccttggagt tcatcgcggc taaggcgctc
     1141 cagcaggccg ctggtgacgg ccacgcacgc gcgctcgggc ttgagcccca cggcaaacgc
     1201 gttgggcacc ggggtatcca tgatgaacac gcgcggcatg gccggcagcg cggcggcaat
     1261 gcgcatttct tccacgatgt tataaagctg cggcgcatct tcgtgggaga cctcgcgcgc
     1321 gttggcggag gagagcagga cggattcgcc gctcatcatg ttgatgagca gcagcacgcc
     1381 ccacacgcag aggccgacaa aagcgccgta ggccatgctc tccggcgcgc cgcccaccag
     1441 cagccccagg ccgccgccta acgccgccaa cagtgcggcc agcatggcaa tgagccacgc
     1501 ggatcgccgc ttgttggcgg caatggcttc ccacatgatg gcgctcatgg cgaggcggcg
     1561 cggagatggc gcgcgttagg cgaacgacac cttgggcacg gcgcgctcgg cttcgtcctt
     1621 cagctcgaag agatcccgct tggagaaacc ggcgggaccg gccaccagat tggaggggat
     1681 ggtctgaatc ttgttgttgt agcgcgtgac gctgtcgtta taggcctgcc gcgcaaacga
     1741 gattttgttt tcggtggagg aaagttcctc ttgcagcgcg aggaagtttt gcgacgcctt
     1801 gaggtcgggg tagcgctcca cgttgaccat caggccgcgc agcgcgccgg aaagctggga
     1861 ttccgccggg ccggcttcgg ccgggccgtg ggccttcatg gcgtcggtgc gggctttggt
     1921 gatggcctcg agggtgccgc gttcatgggc catatagccc ttgacggttt cgatcaagtt
     1981 ggggatcaag tcgtgacgcc gtttgagttg cacgtcaatc tgcgcccagg cgttgtccac
     2041 atagttgcgc aattggacca agccgttata gatggagacg aaccaaagga ccaacaccac
     2101 caataccccg agaagaagca acacaggagc cataaccaat ttcctttcta tgcgcgggcg
     2161 cgccgcgcgc tgaccgagga gaaccatacg catcgcacct cgaaacgcaa gcgcggatat
     2221 tgcagaggaa cgcgctgtgc gcgcgcattt cttgggcgcg gggcgcgcag cgacgagacg
     2281 cgggaacgaa agcgcgcacc tcgcggcggc ttgcggtgcg caggaaaaaa gagggccgat
     2341 gacgccaaag aatggggagt tgcacaaaaa aagacagcga tttcgttcag taaagacggg
     2401 ggtttttgga aaaaataaaa aaatcgcaaa aaaagaggtt gacgggtttg gggcctatgg
     2461 tactcttgcc gccacttgaa tggatcaggt cgggcaaccg gctcgtgaag tgagagtttg
     2521 gttctgttaa aaaatgtggt ctggcccttg acagataagc cagtcgaagg atagattcac
     2581 gacggaatca attaagcgag cagggcaacc tgccgctgcg cggttctgga gtcccttgaa
     2641 cgatagggaa agggacgacg ggattttgcg cgcttttttg ctctttggaa aatggaataa
     2701 acgttcatgt aacaccgctt cgaatcagaa gtggtgtggt aagtacaaac agcttgtgtt
     2761 gagtcataag ttaattctta cgaacggccg gacctaaacc ggccgaaatc gaaaggatca
     2821 gcaactcggc ctggcgggca accgccggag ccacccattt tatatggaga gtttgatcct
     2881 ggctcagaac gaacgctggc ggcgtggatt aggcatgcaa
//
LOCUS       DAOPNP010000167         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_38083_length_2854_cov_7.407288, whole genome shotgun sequence.
ACCESSION   DAOPNP010000167 DAOPNP010000000
VERSION     DAOPNP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38083_length_2854_cov_7.407288"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKI64_10530"
     CDS             <1..427
                     /locus_tag="PKI64_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01471.1"
                     /translation="EWSQTFQPFPAGRFRSSTIPALQKPDKNSHDRIRIVGRGCTPGG
                     LLFPGLGLTGRIVGLEWSQTFQPFPAGRFRSSTIPALQKPDKKSHERIRIGLQGWNGR
                     TFQISGLNSLKISEIVGCPNAELKKSNDLLTTTALSWSF"
     gene            complement(454..843)
                     /locus_tag="PKI64_10535"
     CDS             complement(454..843)
                     /locus_tag="PKI64_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01472.1"
                     /translation="MNLRRMGWSVLLMVMGGLLLGGCGGEGGSDQNYDGTWTGRTSNG
                     GRITFTVEGDWVRTLRLEDPTAQIWFPQPVDIRGSTFAAQYLTETDSSDAVRLQGTFT
                     SATHLDGSYSLRKGSYSLSGTFTATRQ"
     gene            992..1498
                     /locus_tag="PKI64_10540"
     CDS             992..1498
                     /locus_tag="PKI64_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01473.1"
                     /translation="MKKWIIPMLAVGLWLVAGGAQAEHRLGLGANYWVAVDDIKIQDI
                     DKDGLSYLVSYQWRPALIGLQADVEFLPDLFGKDGVAPAAYVLVGKAIYAALGAGMIH
                     LDGGWLDDPFIALKVGLDLELLPSLFLDISASYRYHGTIRELDVEDELKKIDTDTVFL
                     GAAARIAF"
     gene            complement(1512..2279)
                     /locus_tag="PKI64_10545"
     CDS             complement(1512..2279)
                     /locus_tag="PKI64_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01474.1"
                     /translation="MKLRAPSPPRAGFTLVELLTTMAGVGLLAAVLIGLLPRARAAAE
                     SATCRSNLRQLAAANLAYAAEHGRYVTAATDIVGGNSIRWHGVRSGAAAFDGALGPLA
                     EYLGGAGASRWVRRCPALPMVDGGFEASCGGYGYNAHGVGSEVCLGVDNPTRQGMRAG
                     TLAHPARTVMFADTAYMQGGGAQAKLIEYSFAEPPRFAGGGTPWPTIHFRHAGRANVA
                     WCDGHVTSEVLARTGTRGAAQHLGWFGPDDNSFFDPF"
     gene            complement(2276..>2854)
                     /locus_tag="PKI64_10550"
     CDS             complement(2276..>2854)
                     /locus_tag="PKI64_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01475.1"
                     /translation="FPLPITDGPGPDFAVFENAFTEEFLELAFVEVSSDGTNFFRFPN
                     HAFATNPVLGYSDEGGTEADALGGLAGKHLQGHGTPFDLHSLVGTPGLDVRRVTHVRL
                     VDIPGDGSTLDSYSHPIYDPFPTFGSGGFDVNGVGVLNALVEIGTAPNETPPALPGFT
                     TQLEYKASLLDADWLPATDRSAPGFYRWRLSR"
BASE COUNT          556 a          844 c          965 g          489 t
ORIGIN      
        1 ggaatggtcg cagaccttcc agcctttccc ggcgggaagg tttcgttcct caaccattcc
       61 cgccctacaa aagcccgaca agaacagcca tgaccgcatc cggattgtag gccggggttg
      121 cacccccggc gggctgttat ttccgggtct aggattgaca ggtcggattg tagggctgga
      181 atggtcgcag accttccagc ctttcccggc gggaaggttt cgttcctcaa ccattcccgc
      241 cctacaaaag cccgacaaga aaagccatga gcgcatccgg attggattgc agggctggaa
      301 tggtcgcacc ttccagatat cagggctcaa ttcactcaaa atcagcgaaa ttgttgggtg
      361 tcccaatgcg gaactcaaga agagcaacga cctgctcacc acgaccgcgc tgagctggag
      421 cttctaaccg gaatgattca ggtcggcacg ggcttattgg cgtgtggcgg tgaaggtgcc
      481 ggacagtgaa tatgatccct tgcgcagcga gtagctgcca tccaagtggg tggcggaggt
      541 gaacgtgcct tgcaggcgca cggcatccga gctgtcagtt tcggtgagat attgcgcggc
      601 gaacgtactg ccgcgaatat ccaccggctg ggggaaccaa atttgggcag tggggtcttc
      661 gaggcggagc gtgcgcaccc aatcgccttc caccgtaaac gtgatgcggc ccccgtttga
      721 ggtgcggccg gtccatgtgc cgtcgtagtt ctggtcgctg ccaccctcgc cgccgcagcc
      781 gccgagcagc aggccaccca tgaccatcag cagtacactc cagcccatcc gtcgtaagtt
      841 catgcggcat cctttggaat gagcgaactc tactcgccgg gtcggggtgg ggtcaagcgc
      901 cgcgcaaaat ttcagaacgc cggtcttgcc gcggcgcgga caccgtgcaa gaatgctgaa
      961 aaacaaacgg aaccaagcgc aggagggtgt aatgaagaag tggataattc caatgttggc
     1021 ggttgggttg tggttggtgg cgggaggggc acaagccgaa caccggctgg ggctgggggc
     1081 caattactgg gtggccgtgg acgacatcaa gatccaggac atagacaagg atggcctgag
     1141 ctatttggtg agttaccagt ggcgcccggc attgatcggc ctgcaagccg acgtggaatt
     1201 cctgcccgac ctgtttggca aggatggcgt ggcgccggcg gcctacgtgc tggtgggcaa
     1261 agccatctat gccgcgctgg gggcgggcat gatccacctg gatggcggct ggctggatga
     1321 tccgttcatc gcgttgaaag tggggttgga ccttgaactg ctgccaagcc ttttcctgga
     1381 tatctccgcg agctaccgct accacggcac cattcgcgaa ttggatgtgg aagacgagct
     1441 caagaagatt gacaccgata ccgtgtttct tggcgcggcg gcgcggattg cgttctgaga
     1501 cgcgctgcgc gtcagaacgg atcgaaaaac gaattgtcgt cgggcccgaa ccagcccaga
     1561 tgctgcgcgg cgccgcgcgt gccggtgcgg gcgaggactt cgctggtcac gtggccatcg
     1621 caccacgcca cattggcgcg gccggcatgg cggaaatgaa tggtgggcca cggcgtgccg
     1681 ccgccggcaa agcgcggtgg ctcggcaaat gaatactcga tgagcttggc ctgcgcgccg
     1741 ccgccctgca tataggcagt atcggcgaac atcacggtgc gcgccggatg ggcgagagtt
     1801 ccggcgcgca tgccttgacg cgtggggttg tccacgccaa ggcacacctc ggagccgacg
     1861 ccgtgcgcgt tgtagccgta gccaccgcac gacgcttcga agccgccatc caccatgggc
     1921 agggcggggc agcgtcgcac ccagcgactg gcgccggcgc cgccgagata ttccgccagc
     1981 gggcccagcg cgccatcgaa cgcggcggcg ccgctgcgca ccccgtgcca gcggatggag
     2041 ttgccgccga cgatgtcggt ggcggcggtg acataacgcc cgtgctccgc ggcataggcc
     2101 aaattggccg cggccaactg ccgcagattg gagcggcagg tggcggactc cgccgcggcg
     2161 cgggcccgcg gcagcaggcc gatgagcacc gccgccagca agcccacgcc ggccatagtg
     2221 gtcagcaatt ccaccagcgt gaacccggcg cggggggggc tgggcgcgcg gagcttcacc
     2281 ggctcaacct ccagcggtag aagccgggtg cgctgcggtc ggtggccggc agccaatcgg
     2341 catcaagcag gctggccttg tattccaatt gcgtggtgaa gccgggcagg gcggggggcg
     2401 tttcgttggg cgccgtgccg atttccacca gcgcgttcag gacaccgacg ccattcacat
     2461 cgaagccgcc ggacccgaag gtggggaaag ggtcgtagat gggatgactg tagctgtcca
     2521 gcgtggagcc atcgccgggg atatccacca ggcgcacgtg ggtgacgcgg cgcacatcca
     2581 accccggcgt gcccaccaac gagtgcaaat caaacggcgt gccatgacct tgcagatgtt
     2641 tgccggcgag accgccgagg gcgtcggctt cggtgccgcc ttcgtcggaa tagcccagca
     2701 cgggattggt ggcaaaggcg tgattcggaa aacggaagaa gttggtgccg tcggaggaga
     2761 cttccacaaa cgccagctcc aaaaattctt cggtgaaggc attttcaaaa acagcgaagt
     2821 ccggaccggg gccgtcggtg atgggcagcg ggaa
//
LOCUS       DAOPNP010000168         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_39486_length_2778_cov_9.868895, whole genome shotgun sequence.
ACCESSION   DAOPNP010000168 DAOPNP010000000
VERSION     DAOPNP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39486_length_2778_cov_9.868895"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..214)
                     /locus_tag="PKI64_10555"
     CDS             complement(59..214)
                     /locus_tag="PKI64_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOE01476.1"
                     /translation="MSMHRSLKAGSKVATRRNVLKRFERVEVLKKEGRWKEGDRGRGL
                     PKTKPPA"
     gene            complement(338..1486)
                     /locus_tag="PKI64_10560"
     CDS             complement(338..1486)
                     /locus_tag="PKI64_10560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE01477.1"
                     /translation="MKIAFLLQDTRSLYGAEQATVQLLAGLTAAGLAAHVVLLQETRL
                     GAGGSPLADALRQVAPVHSIPVAGRFSRSAVAQIREIMARERCDVLHSTGYKADWHAL
                     LASRRASLFPVVSTVHGWLFRWQPKERFFQALNIRALRQFSRVVVLSQFYERYLRRCG
                     LGPLQLAHIPTGICPERVVGRAVAQELWNAPGEMFTFGMLGRLSSEKDHRLLLRAVRR
                     LARDLDTSPRSWRILIAGDGPLRGALQRQIARWGLRDRVELAGWLPAAEFFARTHVLV
                     QCSRVENQPLSVMEAMAWMRPVLATRAGGLPELVADGETGALVRRSARALAAAMRHYL
                     AAPTKARADGIRARDRLEQQYAVEPMIRDHIGLYATVRASWNTPDNVS"
     gene            1722..2687
                     /gene="rpsB"
                     /locus_tag="PKI64_10565"
     CDS             1722..2687
                     /gene="rpsB"
                     /locus_tag="PKI64_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOE01478.1"
                     /translation="MVTMTTANVRQDVSIQDLLEAGLHFGHRTKRWNPKMRKYLFGQR
                     NGIYIIDLEQTLDGLQKARQFIYDTVARGRQILFVGTKKQAQEALKSVATQTKQPFVV
                     NRWLGGTLTNNETLRKSITRMREIEQMEKDGSINSMPKKEIAKLRHELSKLQYNLSGI
                     ADMQGNPGALFVVDINCEAIAVAEANRLHIPVIALVDANVDPDPVDYPIPANDDAIRG
                     IKLIADLVGLTIQQASSEYSKVAAEEARKRAAEEAAAAAKAKAEAEERKARNAELAKA
                     KAEAREKAKAEKAAAEAAAKAAAEAAPEAAPEAKPEAEPPAAPAE"
BASE COUNT          589 a          945 c          837 g          407 t
ORIGIN      
        1 gccacaaaaa aacctccggg ggcaacccgg aggcttggca caagcttggt acggcggatt
       61 atgccggggg cttggtcttg ggcaaaccgc gcccgcggtc gccctctttc caacggcctt
      121 ccttcttcag gacttctacg cgctcaaaac gcttcaagac gttgcgtcgg gtggccacct
      181 tgctgccggc cttcaaacta cgatgcatgg acatgggtat tctccctcaa acaagttttc
      241 ttctgctgca aatgagtcgc gcacttgtac catttccgca ggtgatgtca actctgcgct
      301 ttgttctttt gcgcggcggg cgcaaaacgc ggggcgttta tgaaacattg tcgggggtgt
      361 tccacgaggc ccgcacggtg gcatacaggc cgatgtggtc gcggatcatc ggttcgacgg
      421 catactgctg ctccagccgg tcccgggcgc ggataccgtc tgcccgcgcc ttggtgggcg
      481 cggccaaata atgccgcatg gcggcggcca acgcgcgcgc cgagcgccgc accagcgccc
      541 cggtctcgcc gtcggccacc agttccggca gcccccccgc ccgcgtggcc agcaccggcc
      601 gcatccacgc catggcttcc atgacgctca acggctgatt ctccacccgc gagcactgca
      661 ccaaaacatg ggtgcgcgcg aaaaactcgg cggcgggcag ccagccggcc agctccacgc
      721 ggtcgcgcaa gccccagcga gcaatctgcc gctgcaatgc gccgcgcaat gggccatcgc
      781 cggcgatcag gatgcgccac gaacggggcg aggtatccaa atcccgcgcc agccggcgca
      841 ccgcgcgcag gagcaggcgg tggtcttttt cggagctcaa ccgccccaac ataccgaagg
      901 taaacatctc gccgggtgcg ttccacaact cttgcgcgac cgcgcggcca accacgcgct
      961 ccgggcagat gcccgtgggg atgtgcgcca gttgcaacgg cccgagtccg cagcggcgca
     1021 agtagcgctc ataaaactgg ctcaatacca ccacgcgcga gaactggcgc aacgcccgga
     1081 tattcagcgc ttggaaaaaa cgttccttcg gttgccagcg gaaaagccag ccgtgcaccg
     1141 tgctgaccac ggggaacagg ctggcgcggc gggaggccag cagcgcgtgc caatcggcct
     1201 tgtagccggt ggagtgcagc acatcgcagc gctcgcgcgc catgatctcg cggatttgcg
     1261 ccacggcgga acgggagaag cggccggcca ccggaatcga gtgcaccggc gccacttggc
     1321 gcagggcgtc ggccagcgga ctcccccccg cccccagccg cgtttcctgc agcagcacca
     1381 catgtgcggc caagccggcg gccgtcaggc cggccagcaa ctggaccgtc gcttgctccg
     1441 cgccatagag gctgcgggtg tcttgcagca aaaacgcaat cttcatcggc cccactctag
     1501 cgaaagcccc ggccaagcgc aaacttccgc accgccaccc tgccgcaaaa aatcacaaaa
     1561 accccttgca ttccagaggg ggttccccta cattccgcgg cgagttttgc gcgtgcaaaa
     1621 ccggaagcga agttccttgg cggccctccg cccgggacaa aagcggattt tccggcgtaa
     1681 caaaaaaagc cggaacaagc cattaaagag aggaaaacat catggtcacc atgacgaccg
     1741 cgaacgtccg tcaggacgtc agcattcagg acctgctcga agcgggtctg cacttcggcc
     1801 accggacaaa acgctggaac cccaagatgc gcaagtatct gttcgggcag cgcaacggga
     1861 tttacatcat tgatctggag caaacgctcg acggcctgca aaaagcccgg cagttcattt
     1921 atgacaccgt ggcccgcggg cgccaaatcc tgtttgtcgg caccaagaag caggcgcagg
     1981 aagcgctgaa gtccgtcgcc acccaaacca agcagccctt tgtggtcaac cgctggttgg
     2041 gcggcacgct caccaacaac gaaacgctgc gcaaatccat cacccgcatg cgcgagatcg
     2101 agcagatgga aaaagacggc tcgatcaact ccatgcccaa gaaggaaatt gccaagctgc
     2161 gccacgagct gtccaagttg caatataact tgagcggcat tgcggacatg cagggcaacc
     2221 ccggcgcgct gtttgtggtg gatatcaact gcgaagccat cgccgtggcc gaagccaacc
     2281 ggctgcacat ccccgtgatt gcgctggtgg atgccaacgt ggatcccgat ccggtggatt
     2341 acccgatccc ggccaacgat gacgccatcc gtggcatcaa gctgattgcc gacctcgtgg
     2401 gcctgaccat ccagcaggcc agcagcgaat attccaaggt tgcggccgaa gaagcccgca
     2461 agcgcgccgc ggaagaagcg gctgccgccg cgaaggccaa ggccgaagcc gaagaacgca
     2521 aggcccgcaa cgccgagcta gccaaggcca aggccgaagc ccgcgagaag gccaaggcgg
     2581 aaaaagccgc ggccgaagcg gcggccaaag ccgccgcgga agccgcgccg gaagcggcgc
     2641 ccgaggccaa gcctgaggcg gaaccgcccg cggctcccgc cgaataaccc tgccggggcc
     2701 ggtcagacgg acgcaccggt gtgtgtccgg ccggccccgc tccccctccc ccccccaata
     2761 gaaaggaata ccccatgg
//
LOCUS       DAOPNP010000169         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_39849_length_2759_cov_2.822115, whole genome shotgun sequence.
ACCESSION   DAOPNP010000169 DAOPNP010000000
VERSION     DAOPNP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39849_length_2759_cov_2.822115"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1511
                     /locus_tag="PKI64_10570"
     CDS             99..1511
                     /locus_tag="PKI64_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOE01479.1"
                     /translation="MLFNSQVFLIFFVVVYGLYRLLPHRWQNVLLLVSSYFFYGWWDW
                     RFLTLIFLSTVIDYIAGGIIYSAGPNQRRRKAAVVVSMTSNLVILGFFKYFNFFADNL
                     SAVLQYAGLPAFTRHLNIILPVGISFYTFQAMSYALDIYRGHLKPARSFLNFATFVAY
                     FPQLVAGPIERASNLLPQIENPRTITRAGIESGAWLIFWGLFKKCVIADNLAVLVDGV
                     FGATGATGATSLLALYAFAFQILCDFSGYSDIARGLARWMGVELMLNFNNPYAALNPK
                     DFWSRWHISLSSWLRDYLYIPLGGNRKGRRRTYINLALTMLLGGLWHGAAWTFVTWGA
                     FHGLLLVIYHRWAEKFSPKGTPDSGRWLRLRRLWMFHLACAGWLFFRANSLGQVGDMV
                     RSLTTNFSWNTDAANMLMAILIYCTPLWLVQWLQVKTGNLSAPMELSFLPKAALLATL
                     VWMFLALGNTGGGAFIYFQF"
     gene            1514..2755
                     /locus_tag="PKI64_10575"
     CDS             1514..2755
                     /locus_tag="PKI64_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01480.1"
                     /translation="MKISPNKPGVREVECAFLRKYWLAFPLLGACSFLAVGGPFWQWV
                     EGASISVHWANLLSWRTLSWAMASLVIPAIWWLGLHFPVRRRWTLLGALGLFLAMDLL
                     LRTSFLQVPLWLAARTRLDSGQYFMREVCHVRLEENAGRQEPRPAVVLVGSSQVLNGV
                     DTDLLRELLAPVPVIRRAMFGMTPLKALAMSSHMPFRAGDHCVLYLSEFDFTNQDEFP
                     FAWFRPYASWRTLPGVLQSMSRRTKTWHWRRVTDYMLAATVQSWQVRDFLQQVAVHFW
                     APASQATRSGNDDVKTVLVTSARAELKHAPAEQRAFLRFAKRLAAHDVDMVIFEGDVN
                     PAIYSPSRMAAKSQVRDFLVELTADSGQRYVSREEQGLAFTAEDWSDMSHLNSVGREK
                     LTRRMAEVLSENTSMKREPTQ"
BASE COUNT          488 a          690 c          847 g          734 t
ORIGIN      
        1 aaatgtgcaa caaatgactg tgctgaacga gatttggaga gttgaatttg tattttgagc
       61 ggaaaacagg cgggaattca gctattgcag gatagaccat gctgtttaat tcccaggttt
      121 ttctcatttt cttcgttgtg gtatatggcc tgtatcggct gttgccgcat cgctggcaaa
      181 atgtgctgct gttggtgtcg agctactttt tttacgggtg gtgggattgg cgttttctga
      241 cgctgatatt tttatccacg gtcattgatt acatcgctgg gggtatcatt tattcggcgg
      301 ggccgaacca gcgccgccgc aaggctgcgg tagtggtcag catgaccagc aacctggtga
      361 tcctcggctt tttcaaatat ttcaactttt tcgcggataa tctgtcggcg gtcctgcaat
      421 atgcgggcct gccggcattc actcggcact tgaacatcat tttgccggtt ggcatttcat
      481 tttacacctt ccaggcgatg tcctacgcgc tggatattta tcgcggccac ttaaagccgg
      541 cccgttcctt cctgaatttc gccacgtttg tggcgtactt cccgcaattg gttgcaggac
      601 cgattgagcg ggcctcgaac ctgctgcccc agatcgaaaa tccccggacc atcacgcgcg
      661 cgggcatcga aagcggcgcc tggctgatct tctggggcct gttcaagaaa tgcgtgattg
      721 cggacaattt ggctgttctg gtggatgggg tgtttggcgc aacgggggcg acgggagcga
      781 cctcgctgct ggcgctgtat gcgttcgctt tccagattct gtgtgatttt tcgggataca
      841 gcgatattgc ccgtggcttg gcgcgctgga tgggcgtgga attgatgctg aattttaata
      901 atccgtatgc ggcgctgaat cccaaggatt tttggagccg ttggcatatc agcctttcga
      961 gttggttgcg ggactatctg tatattcccc tgggggggaa ccggaaaggt cgtcgccgaa
     1021 cgtacatcaa tcttgcgttg acgatgctgc tgggcggtct ctggcacggc gcggcttgga
     1081 catttgtgac ttggggcgct ttccacggct tgctgctggt gatttatcac cgctgggccg
     1141 aaaagttttc acccaagggc acgccggatt ctggccggtg gctgagactg cggcggctct
     1201 ggatgtttca tctggcctgc gcgggctggt tgtttttccg ggccaattcg ctggggcagg
     1261 tgggtgacat ggtgcgcagc ctcacgacga acttttcatg gaatacggat gccgccaaca
     1321 tgttgatggc catactgatt tattgcacgc cattgtggct ggtgcagtgg cttcaggtga
     1381 agaccggaaa tctgtccgca cctatggaat tatcgttttt gcccaaggcg gctttgctcg
     1441 ccaccctggt gtggatgttc ctcgcgcttg gcaacacggg cggcggggct tttatctatt
     1501 tccaattcta atcatgaaga tctcccccaa caaaccaggt gtgcgcgagg tggaatgcgc
     1561 atttctgcgc aagtattggc tggcttttcc tctgctgggg gcatgttcct tcctggctgt
     1621 gggcggcccc ttttggcaat gggtagaagg cgcgtccatc tctgtccatt gggccaatct
     1681 gttgagttgg cgaacgctgt cttgggcgat ggcttctctg gtcatcccgg caatctggtg
     1741 gctcggcctg catttccccg ttcgccgccg ctggaccctg ttgggtgcac tggggttgtt
     1801 tttggcgatg gaccttttgc tgcgaacctc tttccttcaa gttccgctgt ggctggccgc
     1861 ccgtacacgg ctcgattccg ggcaatactt catgcgggag gtctgccatg tccgcttgga
     1921 ggaaaatgcg ggccgacagg aaccgcggcc tgctgtggtt ctggtggggt cgagccaagt
     1981 gctgaacggg gtggatacgg acttgctgcg ggagttgctg gcgccggtcc cggtgatccg
     2041 gcgcgcgatg tttggcatga cgccgctgaa agccttggcc atgtcgtccc atatgccatt
     2101 ccgggccgga gatcattgcg tgctatattt atcagaattt gattttacca atcaggacga
     2161 gttcccattt gcgtggttcc gtccgtatgc ctcgtggcgg acattgcccg gtgtgttgca
     2221 gagcatgtct cgccgcacga aaacatggca ttggcgacga gtgacggatt acatgctggc
     2281 ggcgaccgtt cagagttggc aggtgcgcga tttcctgcag caggttgctg ttcatttttg
     2341 ggcgcccgcc agtcaggcaa cccgttcagg aaacgatgac gtcaagaccg tcttggttac
     2401 cagcgcccga gccgaactca agcatgcccc ggccgaacaa cgggcgtttc tgcggtttgc
     2461 aaagcgcctt gctgcgcacg atgtggacat ggtgattttc gagggggatg tcaatccggc
     2521 catttattct ccatcccgga tggcggccaa gtcgcaggtt cgtgattttc tggtggaact
     2581 gacggcggat tctggccagc gttatgtttc ccgggaagaa caaggtcttg cattcacagc
     2641 ggaagactgg agcgatatga gtcacttgaa ttcggtcggg cgggaaaagc taacgcggcg
     2701 gatggcggaa gtgctgtcgg agaataccag tatgaagcgt gaaccaactc aataatgga
//
LOCUS       DAOPNP010000170         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_41405_length_2681_cov_8.366337, whole genome shotgun sequence.
ACCESSION   DAOPNP010000170 DAOPNP010000000
VERSION     DAOPNP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41405_length_2681_cov_8.366337"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2382
                     /locus_tag="PKI64_10580"
     CDS             <1..2382
                     /locus_tag="PKI64_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01481.1"
                     /translation="PRLELNGPVSVTAGDSKTYTLTRYGPVGDEVNLASDDTDVMTVP
                     ASVTFGYEQDSVTFQATAVAPGTTVLRAGNDDATATPLNVTVNAGGNVIANDHAGNYT
                     IATFTNGANQGFGFGPWDFWNVPATLGDSTEGGGGDLNSSNGLSFKFAGDNNNGWCNA
                     RRNFDGALGVGSVLSFTFTYNWDGGNRGVDIFCSTGQFANLINVVHPDTFQVNGQTIS
                     TNYSPGAVVEVEITQEADGIAMTLTRSVGGTPNLTYTTNIANALPATGFSMYCGGYDY
                     SVDDEAKYAIYMNDLRIVSQSGPVAKLVLSGPTKIWLGEELLPLYTLTRSGEVADLVN
                     LTSSDTGVMTVPATVTFASEENTVTFTGAVVAVGATALTAENDDATSAPLNVLVAERP
                     DYVMYDDASLYAGGAWTATPAHQTGFSDWTVTTSPEMEGSHRGVFIGSSPIEGMDEDG
                     VSFGLYANWDSVVEPDPLPEIKVSRTFPALAVGQTFSVDVGYNWSGGAKGLKLKGTYE
                     GNTFDRIELFNSGNDTWSYKLDGDDQTITVVWDGYVNGGFVGTVQVTCTAENTYDLSF
                     QRAREVAFVVTNVTLPGGIDTVEFYNWNGGSGSEQDFFFNRMGIIGEAAPSLAFIAGT
                     WNPSALGDYQFTLRRTGSTGNEMIVLESDNTNSVTVPAFVWFDDDDATTSFNATVVSL
                     TEGDAKIIASNTLSGVWAEYTVKPWQGGLLPSIDTITFVAGSGQMKFQVPTGYSLIAV
                     EGADCVVVNGGLVWEPLEEDEDFTYVYPEVTILTDAAARKMIRIRLALQVIGP"
BASE COUNT          536 a          857 c          770 g          518 t
ORIGIN      
        1 ccgcgccttg aactgaatgg gccggtgtcg gtcaccgccg gcgattccaa gacctacacg
       61 ctgactcgct acggccccgt cggcgatgag gtcaacctcg ccagcgatga cacggatgtc
      121 atgaccgttc ccgcctccgt caccttcggc tatgaacagg acagcgtcac cttccaagcc
      181 accgccgtgg cccccggcac caccgtgctg cgcgcgggga atgatgatgc caccgccacg
      241 ccactgaacg tcaccgtcaa tgccggcggc aacgtcatcg ccaacgacca cgcgggcaat
      301 tacaccattg ccaccttcac caacggcgcc aaccaaggct ttggttttgg cccgtgggac
      361 ttctggaacg ttcccgcaac gctgggcgat tcgaccgaag gtggcggcgg cgacctgaac
      421 tcttcgaacg gcctctcctt caagttcgcc ggcgataaca acaatggctg gtgcaacgcc
      481 cgccgcaact ttgatggcgc attgggcgtc ggcagcgtgc tgagcttcac cttcacctat
      541 aactgggatg gtgggaaccg aggcgtggat atcttttgct caactgggca attcgccaac
      601 ctgatcaatg tggtacatcc cgatacgttc caagtgaatg ggcagactat ttcgactaac
      661 tattcaccgg gcgcggtggt cgaagtagaa atcacgcagg aggcggacgg catcgccatg
      721 acgctgaccc gctccgttgg cggcacaccc aacctgacct acaccaccaa cattgccaat
      781 gccctccccg cgacgggttt ctccatgtac tgcggcgggt atgactatag cgtggatgac
      841 gaagccaaat atgcgattta catgaacgac ctgcggattg tcagccagtc cggcccagtg
      901 gccaaattgg tgctttccgg ccccaccaag atctggctgg gcgaggaact cctgccgctc
      961 tataccctga cccgttcggg cgaggtggcg gacttggtga acctgaccag ctccgatacc
     1021 ggtgtcatga cggttccggc cacggtcacc ttcgccagcg aagagaacac cgtgaccttt
     1081 accggcgcag tcgtggcggt cggcgcgacg gcgctgacgg ccgagaacga cgatgccacc
     1141 agcgccccgc tgaacgtgct ggttgccgag cgcccggact acgtgatgta tgacgacgcc
     1201 tcgctctatg ccggcggcgc atggaccgcc accccggccc accagacggg cttctccgac
     1261 tggaccgtca cgacctcgcc agaaatggaa ggctcccacc gcggcgtctt catcggttcc
     1321 tcgcccatcg agggcatgga tgaagacggc gtctcgttcg gcctctacgc caattgggac
     1381 agcgtggtcg aacccgatcc gctgccggaa atcaaagtat cgcgcacctt ccccgccttg
     1441 gcggtcggcc agaccttctc ggtggatgtg ggctacaact ggagcggcgg cgccaagggg
     1501 ctgaagctca agggcaccta cgaaggcaac accttcgatc gcatcgagct gttcaactct
     1561 ggcaacgaca cctggtccta caagctcgac ggcgacgatc aaaccatcac ggttgtctgg
     1621 gatgggtatg tcaatggcgg ctttgtcggc acggttcagg tcacctgcac cgcggaaaac
     1681 acctatgacc tctccttcca gcgcgctcgg gaggtggcct tcgtcgtgac caatgtcacc
     1741 ctgccgggcg gcattgacac cgtcgagttc tacaactgga acggcggcag cggcagcgag
     1801 caggatttct tcttcaaccg catgggcatc atcggcgagg ccgcgccctc gctcgcattc
     1861 atcgccggca cgtggaaccc ctccgcgctg ggcgactacc agttcaccct ccgccgcacc
     1921 ggctccacgg gcaacgaaat gattgtcctg gaaagcgaca acaccaactc tgtcaccgtg
     1981 ccggcgttcg tttggttcga tgacgacgat gccactacgt ccttcaacgc gaccgtcgta
     2041 agcctgaccg agggtgatgc caaaatcatc gccagcaata cgctcagcgg cgtgtgggcg
     2101 gaatataccg tgaagccttg gcagggcggg ctgctgccct cgattgacac catcaccttt
     2161 gtggccggca gcggtcaaat gaagttccag gttcccaccg gctactcgtt gatcgctgtg
     2221 gaaggcgcgg actgcgtggt ggtcaatggt ggcctcgtct gggagccgct ggaggaggac
     2281 gaggacttca cctacgtcta ccccgaagtg accatcctga ccgatgccgc cgcgcgcaag
     2341 atgattcgta tccgccttgc cctgcaggtg atcgggccat aatcaacgca acatgacgtt
     2401 gccacgccgg ccccgccccg cggggccggc tttttcatgg ccttgccctg ctttttccac
     2461 actgtggaaa aatcacctca aagttcgtcc attgtgtgga aaagtggggg caaagttgtt
     2521 ccattgcgtg gaaaaatcag ccccattttt ccacagcgtg gaaaatattt atcccgcgtg
     2581 cgcgggattt ttccacagcg tggaaaaaat cggacctcca gccttggatt tttccacaga
     2641 taggatgaga aggcaaaaga ggatcggttt cctttgggcg t
//
LOCUS       DAOPNP010000171         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_42526_length_2627_cov_8.694790, whole genome shotgun sequence.
ACCESSION   DAOPNP010000171 DAOPNP010000000
VERSION     DAOPNP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42526_length_2627_cov_8.694790"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..712)
                     /gene="hpt"
                     /locus_tag="PKI64_10585"
     CDS             complement(170..712)
                     /gene="hpt"
                     /locus_tag="PKI64_10585"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOE01482.1"
                     /translation="MTAYHINDILITPMELALRVDELAREIAAGGPSENLAVIGILRG
                     SFIFLADLVRWFDHNGLRPRIDFMIMESYGKGTAPVREPVVTRDCSIDLTGADVVVVD
                     DILDTGRTLQKAVAHLQAKGARSVRTCVLLDKPSRRVVPCQADYVGFAIDDHFVVGYG
                     LDYAGYHRELPHIAKVTFTE"
     gene            796..1530
                     /locus_tag="PKI64_10590"
     CDS             796..1530
                     /locus_tag="PKI64_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01483.1"
                     /translation="MKTLRTVSGWMLLGGLLLAMAGALAGCDVDSVDSTTAVPSDNSG
                     KIYNYSGLYLPAGTNVDLLVFPTNRQSGVKLTWLRLLQYGTVLEGYDNANKTWTGKIS
                     SINEGNASFTLEGTTTAGNHVNVAGSLRYADQNSTMDATWIEPGFSGSIFARAAVSAP
                     STNTPITSLSISPTSATLSIGNSTRTFTASGGSGNYTWTHSNASCGSLSATTGNSITY
                     TRLAAGTDTLTVSSEGFVATASITCQ"
     gene            1527..2510
                     /locus_tag="PKI64_10595"
     CDS             1527..2510
                     /locus_tag="PKI64_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOE01484.1"
                     /translation="MKKKKISPRCAMGAPEADICRTARELVGLFNCEALDAPTAVLTH
                     SPAPKNVVDGLRVLLDVMFPGKVSASLADGSELGVFLIRRLSEAWRLLHREIRRALPY
                     RWIGEAARTAGVRPPKVANLDRETDRILTAFFAALPKVRRLLIDDAQAAYDGDPAALT
                     FAEVLLSYPGLLAIAAHRLAHELYNLRVPIIPRIMSEWIHTKTGTDIHPGARIGRSFF
                     IDHATGVVIGETTQIGNRVKLYQGVTLGARSFALGATGLPVKHIKRHPTVRDDVIIYA
                     HATILGGGTVIGARSIIGSNVFLMESIPADSVVSSNHPELSIREKKRTKKT"
     gene            2550..2625
                     /locus_tag="PKI64_10600"
     tRNA            2550..2625
                     /locus_tag="PKI64_10600"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2583..2585,aa:Lys,seq:ctt)
BASE COUNT          494 a          957 c          733 g          443 t
ORIGIN      
        1 catccacttc gaggttgcgc atggggcgcg ggggcggttg ctcgccggcg gggcgcacgc
       61 ggtggaaggt cagcaccacc agcttgccgg cgtgttggcg acgcaggccg ccggcggcgg
      121 cataggcccg cgcccacgtg gctttcagga acgtgcgcat gccgtcaatc tactcggtga
      181 aggtcacctt ggcaatgtgc ggcagctcgc ggtggtagcc ggcgtaatcg aggccgtagc
      241 ccaccacaaa gtggtcgtca atggcaaaac cgacataatc ggcctggcag ggcaccacgc
      301 ggcggctggg tttgtccagc agcacgcagg tgcgcaccga gcgcgcgccc ttggcttgca
      361 ggtgtgccac cgccttctgg agcgtgcggc cggtgtcgag gatgtcgtcc accaccacca
      421 cgtccgcgcc ggtgaggtca atgctgcaat cgcgcgtgac caccggctcg cgcacgggcg
      481 ctgtgccctt gccgtagctt tccatgatca tgaaatcaat gcgcgggcgc aggccgttgt
      541 ggtcgaacca ccgcacaagg tccgccagga aaataaaact gccgcgcaaa atgccgatga
      601 ccgccaggtt ttcgctgggc ccgcccgccg cgatttcccg cgccagctcg tccacccgca
      661 gcgccaattc catcggggtg atcaaaatat cgttgatgtg gtaagctgtc atgcgtgagc
      721 ctccgccgcg aacactcttg caaaaaggcc ccggatgggg gaagctaaaa ggatatgcaa
      781 caaggaaacc ccgctatgaa aacgctgcgt acggtgtcgg gttggatgct tttgggcggc
      841 ctcctgctgg ccatggccgg cgcgctggcc ggctgcgatg tggattcggt ggatagcacg
      901 accgccgtac cctccgacaa ctccggcaaa atctacaact attccgggct ctatttgccc
      961 gccggcacca acgtggacct gctggtgttc cccaccaacc gccaaagtgg cgtcaaactg
     1021 acgtggctgc gcctgctgca gtacggcacc gtgctggaag gctacgacaa cgccaacaaa
     1081 acgtggaccg gcaaaatctc cagcatcaac gagggcaacg cctcgttcac gctggaaggc
     1141 accactaccg ccggtaacca cgtgaatgtg gccggctccc tgcgctatgc cgatcaaaac
     1201 tccaccatgg atgccacgtg gattgaaccc ggtttctccg gcagcatctt cgcccgcgcc
     1261 gctgtctccg cgcccagcac caacacgcca attacttcat tgagcatttc gcccaccagc
     1321 gccacgctca gtatcggcaa ctccacgcgc accttcaccg ccagcggcgg ctccggcaat
     1381 tacacgtgga cccacagcaa cgcatcctgc ggctcgctct ccgccaccac cggcaactcc
     1441 atcacctata cccgcctggc cgccggcacc gataccctca cggtctcctc cgaaggattc
     1501 gtcgccaccg ccagcatcac ttgccaatga aaaagaaaaa aatctccccg cgctgcgcca
     1561 tgggcgcgcc ggaagcggac atctgccgca ccgcgcgcga actggtcggc ctcttcaact
     1621 gtgaggcgct cgatgcgccc accgccgtgc tcacccacag ccccgccccc aagaacgtgg
     1681 tggacggcct gcgcgtgctg ctcgatgtca tgttccccgg caaagtcagc gcctccctgg
     1741 ccgatggctc cgaactcggc gtgttcctca tccgccgcct cagcgaagcc tggcgactgc
     1801 tgcaccgcga aattcgccgc gccctgccct accgctggat cggcgaagcc gcccgcaccg
     1861 ccggcgtccg cccgcccaag gtcgccaatc tcgaccggga gaccgaccgc atcctcaccg
     1921 cctttttcgc cgcgctgccc aaagtgcgcc ggctgctgat tgatgacgcg caggccgcct
     1981 acgatggcga ccccgccgcg ctcactttcg cggaagtgct gctctcctat cccggcctgc
     2041 tggccattgc cgcgcaccgc ctggcccatg agctctataa cctgcgcgtg cccatcatcc
     2101 cgcgcatcat gagcgaatgg atccacacca agaccggcac cgacatccac cctggcgcgc
     2161 gcatcggccg ctcgttcttc attgaccacg ccaccggcgt cgtcattggc gaaaccacgc
     2221 aaatcggcaa ccgcgtcaag ctgtaccagg gcgtcaccct tggcgcgcgc tcttttgcgc
     2281 tcggcgccac cggcctgccc gtcaaacaca tcaagcgcca ccccaccgtg cgcgacgacg
     2341 tcatcatcta cgcccacgcc accatcctcg gcggcggcac cgtcatcggc gcgcgttcca
     2401 tcatcggctc caacgtcttt ctcatggaaa gcatccccgc cgacagcgtg gtctcctcca
     2461 accaccccga actctccatc cgcgaaaaaa agcgcacaaa aaaaacttga atcccctccc
     2521 gcgtggggta ggatgccccc gcagttgatg gaggcgtagc tcagttggaa gagcaactga
     2581 ctcttaatca gtgggtcgtg ggttcgagcc cctccgcctc taccatc
//
LOCUS       DAOPNP010000172         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_42590_length_2624_cov_8.479953, whole genome shotgun sequence.
ACCESSION   DAOPNP010000172 DAOPNP010000000
VERSION     DAOPNP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42590_length_2624_cov_8.479953"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1849)
                     /locus_tag="PKI64_10605"
     CDS             complement(140..1849)
                     /locus_tag="PKI64_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE01485.1"
                     /translation="MKKSTAWLIFGLTTLALSVLFFWPLGRVVSGGFWVDGHFTWRYL
                     RGVFSNPIYVEGLFNSLKIALGTTSFAMVVALPLAWLANQFEFRGKAIFSALVLVPMI
                     LPPFVGAIGFQQIFGAYGMVNTLFGLGARDWLGGGQYAGVVLLQTLSLYPILYLNAVA
                     ALANIDPAMEEAAANLGCTRLRRFFRITLPLMMPGLFAGGTLIFIWSFTELGTPLIMN
                     YTRCAPVQIYDSLKEVGSNPFPYALVSVMLAASVGLYAVSRWLFGGKSYAMQSKASTQ
                     SAVRRLTGARALLAALPFAVVTGLALLPHLGVILTSFAAPGTWYQTVLPGTWSGANYM
                     AALGHDMTVSSIRNSLLFSSVAVSIDIVAGIAIAFVVVRSNLRLRGVLDALAMIPLAV
                     PGLVMAFGFMSVSSSLANWKTMADHDWWLKLVDVRTNPTLFLVLAYSVRRLPYMVRSA
                     VAGLQQTSVTFEEAAWNLGASPAVTLRRITLPLILANLIAGALLTFAFSMLEVSDSLM
                     LAQQADYMPITKAIFELFQLLGTGKYVAAALGVWAMAFLTATLIASSLLLGKKLGAVF
                     RVS"
     gene            complement(1846..>2624)
                     /locus_tag="PKI64_10610"
     CDS             complement(1846..>2624)
                     /locus_tag="PKI64_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOE01486.1"
                     /translation="GANARYFTDSASKVPLDVGMGNAAAGLCIDFYGRFEADVSNGGR
                     PDGAMAYVTPVGESGVSADPVSLLRGAPHRELALRFIEFTLSEAGQQLWCYRRGAPGG
                     PQQYSLQRFPIRRDFYPAANPQFQANYERHREFTTDDLGQPHTDMYRLAHDFPYQARW
                     TGGYFGLFRDLIRAMCMDSGRELHAAWGAIIAAGGPEKCPRAMAALERLPQEPEPLTW
                     ASGLSMGRKYDRLDLLRDWTLHFRTQYAAAARLAKEEGRP"
BASE COUNT          555 a          863 c          805 g          401 t
ORIGIN      
        1 ctacaatcca acccggctgc ttttgtaggg cgggaatggt tgaggcacga gaccttcccg
       61 ccgcgaaagg ctggaaaggc ggcgactatt ccatccttgc aatccaactg aaatcagttg
      121 gttggccggt gcgcaaaact caggacaccc ggaacaccgc gccgagtttc ttgcccagca
      181 gcaggctgct ggcgatcagg gtggccgtca ggaacgccat ggcccaaacc cccagtgcgg
      241 cggccacgta tttgccggtg ccgagcagtt ggaacagctc gaagatggcc ttggtgatgg
      301 gcatatagtc ggcctgctgc gccagcatga ggctgtcgct gacttccagc atgctgaagg
      361 caaaggtcag cagcgcgccg gcaatcaggt tggccaggat gagcggcagc gtgatgcgcc
      421 gcagcgtgac cgccggcgag gcgcccaggt tccaggcggc ttcttcaaac gttacgctgg
      481 tttgctgcaa gccggccacc gcgctgcgca ccatataggg cagccggcgc acggaatagg
      541 ccagcaccaa aaagagcgtg ggattggtgc gcacatccac cagcttcagc caccagtcat
      601 gatcggccat ggttttccag ttggccagcg aggagctgac cgacatgaat ccgaaggcca
      661 tcaccagccc tggcaccgcc agcggaatca tggccaaggc atccagcaca ccgcgcaggc
      721 gcaggttgga gcgcaccacc acaaatgcaa tggcaatgcc ggccacgatg tcaatgctga
      781 ccgccaccga cgaaaacagc aggctgttac ggatgctgct gaccgtcatg tcgtgcccca
      841 gcgccgccat gtagttcgcg cccgaccacg tgcccggcag gacggtttgg taccacgtgc
      901 ccggcgccgc aaagctggtc aaaatcacgc ccaaatgcgg cagcagcgcg aggccggtca
      961 ccacggcgaa cggcagcgcc gccagcagcg cacgcgcgcc cgtcagccgg cgtacggcgg
     1021 attgcgtgct ggccttgctt tgcatggcgt agctcttgcc gccgaacagc cagcgcgaca
     1081 ccgcataaag gccgacgctc gccgccagca tcaccgatac caacgcatac gggaacgggt
     1141 tggagccgac ttccttcagc gagtcataaa tctgcaccgg ggcgcagcgc gtatagttca
     1201 tgatgagcgg cgtgcccagc tcggtgaagc tccagatgaa aatcaatgtg ccgccggcga
     1261 acaagccggg catcatcagc ggcagcgtaa tccggaaaaa ccggcgcagg cgggtgcaac
     1321 cgaggttcgc cgcggcttct tccatggccg gatcaatgtt ggccagcgcg gcgaccgcgt
     1381 tgagatacag gatgggatag agcgacagcg tttgcagcag caccacgccg gcatattggc
     1441 cgccgcccag ccagtcgcgc gcccccagcc cgaacaacgt gttcaccatg ccgtacgcgc
     1501 cgaaaatctg ctggaagccg atcgcgccca cgaacggcgg caggatcatc ggcaccagca
     1561 ccagcgccga gaaaatcgcc ttgccgcgga actcgaactg gttggccagc caagccagcg
     1621 gcaaagccac caccatcgcg aagctggtcg tgcccagcgc aattttcagg ctgttgaaca
     1681 agccttccac atagatgggg ttgctgaaca caccgcgcaa ataacgccac gtgaagtggc
     1741 catccaccca gaaaccgccc gacaccacac gccccagcgg ccagaaaaac aacaccgaca
     1801 acgccagcgt ggtcagcccg aaaatcagcc atgccgtgct ctttttcatg gccggccctc
     1861 ctctttggcc aaccgggcgg cggcggcata ttgggtccgg aaatggaggg tccaatcgcg
     1921 cagcaaatcc agccggtcat atttgcggcc catgctcagg ccggaggccc aggtcaacgg
     1981 ctccggctct tgcggcaggc gttccagcgc ggccatggcg cgcggacatt tttcaggacc
     2041 gccggcggcg atgatcgccc cccacgccgc atgcagctcc cgtccggagt ccatgcacat
     2101 ggcgcggatc aaatcgcgga acaagccgaa gtagccgccg gtccagcgcg cctgatacgg
     2161 gaagtcgtgc gccagccggt acatgtcggt gtgcggctgt ccgaggtcat cggtggtgaa
     2221 ctcgcggtgc cgttcatagt tcgcctgaaa ttgcggattg gcggcaggat aaaaatcgcg
     2281 gcggatggga aagcgttgca gcgaatactg ctgcgggccg ccgggtgcac cgcgccggta
     2341 gcaccacaac tgctggcccg cttcgctcaa cgtaaactcg atgaaacgca gtgcaagctc
     2401 ccgatgcggc gcgccacgca gcagtgaaac cggatcggcg ctgactcctg attcacccac
     2461 gggggtcaca taggccatcg cgccatcggg ccggccaccg ttgctgacat ccgcctcgaa
     2521 gcggccataa aaatcaatgc acagccccgc cgcggcattg cccatgccca catccagcgg
     2581 caccttgctg gcgctgtcgg tgaagtaccg cgcgttggcg ccga
//
LOCUS       DAOPNP010000173         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_44550_length_2538_cov_8.472010, whole genome shotgun sequence.
ACCESSION   DAOPNP010000173 DAOPNP010000000
VERSION     DAOPNP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44550_length_2538_cov_8.472010"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1428)
                     /gene="hflX"
                     /locus_tag="PKI64_10615"
     CDS             complement(148..1428)
                     /gene="hflX"
                     /locus_tag="PKI64_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOE01487.1"
                     /translation="MTRLHTTQRDDVERAWLVATTLGNQPRAVVEESLRELRALADTA
                     QAQVAGETLCRLRTIQPAMYIGRGKAEEIAAEAKEACANLIIFDDDLSPAQGRALEDV
                     FGVRVVDRTQLILDIFAQRALTLEGRLQIELAQLHYALPRLRGLWTHLERQQGGIGLT
                     GPGEKQLELDRRRIEQRITRLRDQLQAVRNRRAELRRGRRRHGWALVTLVGYTNAGKS
                     TLMNRLTDANVLADDQLFATLDPTTRQLQLPNNQLCLLTDTVGFIRKLPHHLVESFMA
                     TLEEVNEADLLLHVIDAAQPRVDEQIAAVETVLRELGAHQKPLLAVFNKTDLERAHNQ
                     ALRLAEHFQHSVAVSAVTGEGLATLGQMIADLLRDRVTHLHLRIPAAEGRLLAALHAN
                     GKVLSQKISGKHLRIEALVSAAFAGTLDPKWIES"
     gene            complement(1442..2248)
                     /locus_tag="PKI64_10620"
     CDS             complement(1442..2248)
                     /locus_tag="PKI64_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01488.1"
                     /translation="MISSGWRVVAWWLVAGGLAGLPPADAATTNLVHAGGQITVTVTT
                     KQTEIPPLVVTCWPLAAKAVESLVGLPEEPASLTIRLREPPSFGRRLGTFWQEEVFAI
                     QHGDVIEIYAEKDPLKLSFRLAHELCHWVVAQRYPAQLPLWLDEGLAQMAGDAAAQTA
                     ARVHKQSLLRPEPLKLSGNLLTLRELTALTTYPAGRHRVAAFYWQAEALTRHLHQRLG
                     AKEFAEYLTLLGTPTPPTWQAPLRERWYFSDDDFAWLDERLHPQPAGGTP"
BASE COUNT          515 a          792 c          728 g          503 t
ORIGIN      
        1 tacattgcat cccaatgtgt tatggcttct tagattggaa gtgtcgaact caggtccaga
       61 cgttcccggc aggaaggttt cgcatctcaa ccattcccgc ccaacaaaac gaacgaaatt
      121 gttgggtggc ccaatgcgga actcaagtta tgactcaatc cacttgggat cgagcgtgcc
      181 ggcaaatgcc gctgaaacca gagcttcgat tcgcaggtgt ttgccgctga ttttttgcga
      241 caacaccttg ccgttcgcgt gcagggcggc cagtaaccgg ccttccgcag ccggaatccg
      301 caagtgcaga tgtgtcaccc ggtcacgcag caaatcggcg atcatttggc ccaacgtggc
      361 caagccctcg ccggtcacgg cggaaaccgc cacggaatgc tggaaatgtt ccgccagccg
      421 cagcgcttga ttgtgggcgc gctccagatc agttttgttg aagactgcca agaggggttt
      481 ctgatgcgca cccagttcac gcaaaacggt ttctaccgcg gcaatttgct catctacgcg
      541 cggctgcgcg gcatcaatca catgcaatag caagtcggcc tcgttcactt cttccagtgt
      601 ggccatgaat gattccacca agtggtgcgg cagcttgcgg ataaaaccga ccgtatcggt
      661 tagcaggcag agctggttgt tgggcagttg caactggcgg gtggtgggat caagtgtggc
      721 aaagagctgg tcatcggcca atacgttggc atcagtcagt cggttcatca gggtggactt
      781 cccggcattg gtatagccca ccaacgtcac caacgcccag ccatggcgtc gccgcccccg
      841 tcgcaactcc gcgcgccgat tgcgtaccgc ctgcaactgg tcgcgcaacc gcgtaatgcg
      901 ctgctcaatg cgccgtcggt ccagctccag ttgcttttcg cccggcccag tgaggccaat
      961 gcctccctgc tgccgctcca ggtgcgtcca taggccgcgc aatcgaggca gggcataatg
     1021 cagttgcgcc agctcaattt gcaggcgccc ctccaacgtt agggcgcgtt gtgcaaaaat
     1081 atccagaatc agttgggtgc ggtccaccac gcgcaccccg aacacgtctt ctagtgcgcg
     1141 cccttgggcc ggcgacaaat cgtcatcaaa aataatcagg ttggcgcagg cctctttggc
     1201 ttctgcggcg atttcttccg ccttgccacg gccgatatac atcgccggct ggatcgtgcg
     1261 caaccggcac aaggtctcgc ccgccacttg tgcttgcgcg gtatcggcca gcgcgcgcag
     1321 ttcgcgcaac gactcctcaa caacagcgcg cggctgattg cccagcgtgg tggccaccag
     1381 ccaagcccgt tcgacatcat cgcgttgggt cgtgtgtagg cgtgtcatgc ccgttgcccg
     1441 attatggtgt cccgccggcg ggctgcggat gaagacgctc atccagccac gcgaaatcat
     1501 cgtcggaaaa ataccaacgt tcgcgcagcg gcgcctgcca tgtgggcggg gtgggggtgc
     1561 ccagcagggt caagtactca gcgaactcct ttgccccgag ccgttgatgc aggtggcggg
     1621 tcagtgcctc cgcctgccaa taaaacgcgg ccacccggtg ccgaccggcg ggataggttg
     1681 tcagcgcggt caactcccgt aacgtcagca ggttaccgga cagtttgagc ggttccgggc
     1741 gcagcaacga ttgcttatgc acgcgggctg cggtttgggc cgcggcatcg ccagccattt
     1801 gggccagccc ttcgtccagc cagagcggta attgtgcggg gtagcgctgg gccactaccc
     1861 aatggcacag ctcgtgggcc aaccggaatg ataacttcag cggatccttc tccgcgtaaa
     1921 tctcgatcac atccccatgc tgaatagcaa atacctcttc ctgccaaaat gtgcccaacc
     1981 gccgaccaaa cgaaggcggt tcccgcagcc ggatggtcag gctggcgggt tcctccggca
     2041 accccaccaa cgactccact gccttggccg ccaaaggcca acaagtcacg accaacggtg
     2101 gaatctcggt ttgtttggtt gttaccgtta cggtgatttg cccccccgca tgcaccagat
     2161 tggtcgtggc cgcatcggcc ggcggcagcc cggccagccc accggccacc agccaccacg
     2221 ctacgactct ccatccggag ctaatcatgc tcggactata tcgcaattgc cctccgccgc
     2281 aataaaaaca catggacaac cacccgcgcg gcaggagtag ttcagcggta gaatgtcagc
     2341 ttcccaatcc cgctgaagcg ggacagggct gcacacttgt ccttccagtg gcggggccac
     2401 gggttgaaag cacttctgat tcgactactg acgatcaaaa acacggtgga caagcaaccg
     2461 gggctagggt agatatgcgt ctactggatg cgggagtaat tcagcggtag aatgtcagct
     2521 tcccaagctg aacgtcgc
//
LOCUS       DAOPNP010000174         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_44754_length_2529_cov_12.934519, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000174 DAOPNP010000000
VERSION     DAOPNP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44754_length_2529_cov_12.934519"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKI64_10625"
     CDS             complement(<1..1043)
                     /locus_tag="PKI64_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE01489.1"
                     /translation="MLRNIIILAVLAVILALPFAFRQDLGAREWQPGDPVLVIITPMN
                     EAIRYEFALGFSRWHAAHYGRPVKVDWRNIGGSTEIMRYLASEFTASFRAWWTGQGGA
                     WRPDAQSIILSRTFSSERRPADISDADWAAQCALFNAFRQTDDPHKFTSQIDLMFGGG
                     SFDGDNATRQGLLVPPWAQGEIPPGLIATADGAELIPTGLSGDTWRTPTYFGTTLSTF
                     GICYNRDRMRAQHIAAEPRQWEDLANPQWFGTLGLADPTKSGSIAKAFETVVQVQCRR
                     AVIAAGYGEQIDDFEQQIATAKLPDGEMPPGVPAAYQEAVAAGWENGVRLIQKFGANA
                     RYFTDSASKVPLDV"
     gene            complement(1071..2144)
                     /locus_tag="PKI64_10630"
     CDS             complement(1071..2144)
                     /locus_tag="PKI64_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE01490.1"
                     /translation="MSVPITIEKLTKRFGDNTVLKGIDVRIAAGELFFLLGPSGCGKT
                     TLLRAIAGLHEPDEGRILAGDRDITTLPAHERDMGMVFQSYALWPHMTLRENVSFGLE
                     MRRVKRAEIRERAMRALALVKLADRAESKPNELSGGQQQRVALARALVIEPQCLLLDE
                     PLSNLDAKLRLEMRSEIRRICKEAGLTAIYVTHDQKEALSIADRLAVLDQGNLLQVGA
                     PQEVYTRPSNRFVAQFIGETTFIEGHVRAAQGELLTVETAAGPWQAHGAPGMAVGQRV
                     WLSVRPEAVQMHTDRAAGPNVFAARMHSTIYLGEVAQHQVELAGGVTLKALETRPKQV
                     ARDNTSLETRLYLAPEDVQALAN"
BASE COUNT          471 a          805 c          765 g          488 t
ORIGIN      
        1 acatccagcg gcaccttgct ggcgctgtcg gtgaagtacc gcgcgttggc gccgaacttt
       61 tgaatcaggc gcacgccgtt ttcccagccc gccgccacgg cctcctgata ggccgctggc
      121 acgccgggcg gcatttcgcc atccggcaat ttggccgtgg caatttgctg ttcgaaatca
      181 tcaatctgtt cgccgtagcc ggccgcgatg accgcgcggc ggcattgcac ctgcaccacg
      241 gtttcaaatg ccttggcaat gctgccgctc ttggtggggt ccgccaagcc cagcgtgccg
      301 aaccactgcg ggttggccaa atcttcccac tggcgcggct cggccgcgat gtgctgcgcg
      361 cgcattcggt cgcggttgta gcaaatgccg aaggtactca acgtggtgcc gaaataagtc
      421 ggcgtgcgcc aggtatcgcc gctcaaacca gtggggatca gctccgcgcc atcggccgtg
      481 gcaatcaggc cgggcgggat ttcgccctga gcccacggtg gcacaagcag gccttgccgc
      541 gtggcgttgt cgccatcaaa ggaaccgccg ccgaacatca aatcaatttg gctggtgaac
      601 ttgtgcggat catccgtttg ccggaaggca ttgaacagcg cgcattgcgc ggcccagtcg
      661 gcatcgctga tgtccgccgg gcgccgctcc gacgaaaacg tgcggctgag aatgatggat
      721 tgcgcatcgg gccgccacgc gcccccctgc cccgtccacc acgcgcggaa gctggcggtg
      781 aactcgctgg cgaggtagcg catgatttcc gtcgagccgc cgatattgcg ccagtccacc
      841 ttgaccggcc ggccgtagtg cgccgcgtgc caccgcgaaa accccagcgc gaattcgtag
      901 cggatggcct cgttcatcgg agtaatgatg accagcaccg ggtcgcccgg ctgccattcg
      961 cgcgcgccca agtcctgccg gaaagcaaac ggcaaagcca gaatgacggc cagcaccgcc
     1021 agaatgatga tatttcgcaa cataccgaac ccttctattt ccagaccggg tcagttggcc
     1081 agcgcctgca cgtcttcggg cgcgaggtag aggcgggttt ccagactggt gttgtcgcgc
     1141 gccacttgct tcgggcgggt ttccagcgcc ttgagcgtga cgccgccggc cagctccact
     1201 tgatgctgcg ccacttcgcc cagatagatg gtgctgtgca tgcgcgcggc aaacacgttc
     1261 ggccccgcag cgcggtcggt gtgcatttgg acggcctcgg gccgcacgga aagccagacg
     1321 cgctgcccca ccgccatgcc gggcgcgcca tgcgcttgcc acgggcccgc ggcggtttcc
     1381 acggtcagca gctcgccctg cgccgcgcgc acgtgacctt caatgaaggt ggtttcgccg
     1441 atgaactgcg caacaaagcg gttgctgggc cgcgtataca cctcctgcgg cgcgcccacc
     1501 tgcaggagat tgccctgatc caaaacagcg aggcggtcgg cgatggacag cgcctccttt
     1561 tggtcgtgcg tcacatagat cgcggtgaga ccggcctcct tgcaaatgcg ccggatttcc
     1621 gagcgcattt caaggcgcag tttggcatca agattcgaga gtggctcatc caacagcagg
     1681 cattgcggtt caatcaccag cgcccgcgcc aaggcgacgc gctgttgctg gccgccggaa
     1741 agttcgttgg gcttggattc agcgcggtcg gccagcttga ccagcgcgag agcgcgcatg
     1801 gcgcgctctc ggatttccgc gcgcttcacg cgacgcattt cgaggccgaa ggacacattc
     1861 tcgcgcagcg tcatgtgcgg ccaaagcgcg tagctttgga acaccatgcc catgtcgcgc
     1921 tcgtgcgcgg gcagcgtggt gatgtcgcgg tcgccggcga ggatgcggcc ttcgtccggc
     1981 tcgtgcaacc cggcgatggc gcgcagtagc gtggttttgc cgcagcccga cggccccagc
     2041 agaaagaaca gttccccagc cgcaatccgt acatcaatgc ctttgagcac cgtgttgtcg
     2101 ccaaaccgct tcgtcaattt ttcgatcgta atgggcaccg acatggcggg acctcctttc
     2161 gactgccggt tgttatatca caccgccccc cggcggagga tagagatatc tagcctttct
     2221 gttcttcccg cttgagttgc ggcagcgtgg gttttccacg ctgtggaaaa atccacagcc
     2281 gcgcgcccca ttttttccac gctgtggaaa aaagttttcc acactgtgga aaaattcggg
     2341 gccgaacgcc cacttttttc cacgcaatgg aaaaactttc agcgattttt ccacacaatg
     2401 gaacaacttt gggccaaaag ttccacagat aggatgagaa ggcaaaagag gatcggtttc
     2461 ctttgggcgt tgatttaaac acctatctca aacgcactca tcaggtgcag aaaggaaacc
     2521 gatcgtgaa
//
LOCUS       DAOPNP010000175         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_45733_length_2487_cov_5.831414, whole genome shotgun sequence.
ACCESSION   DAOPNP010000175 DAOPNP010000000
VERSION     DAOPNP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45733_length_2487_cov_5.831414"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1282
                     /locus_tag="PKI64_10635"
     CDS             30..>1282
                     /locus_tag="PKI64_10635"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE01491.1"
                     /translation="MIAPFPDSAAADSRPLREVVGEFFALGGALADVTTGDFEFEARP
                     QQQQMADAVAAALADERHLIVEAGTGVGKSLAYLVPLLLHAQQQGIRVLVSTHTIALQ
                     EQLLGKDLPMLHERLGFEFRAVLVKGRMNYLCRRRLARARSHQRELFGDGVAEELERI
                     RTWADKTEDGSLQEWRHEPSAEAWGAVCAEHGNCLGAKCPERKRCFLLRARAKMQDAH
                     LLVANHHLLFSDLALRQDGAAFLPDVGAVVLDEAHMVEDTASEHLGLRLSTYGFEHWL
                     RRLFVPESGKGLLGYLRAGAAARVATELWPAVADLFHEVPRLVGLGPHDGQKVVAQPL
                     QLVTNVPELMHQLSTELEILGEDLVGQDEDSRAELRSLRGQGIALSKMLEAFLDQSMP
                     DHVYWLEREGRRRQPVLYSAPIEVAP"
     assembly_gap    1283..1382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1383..1766
                     /locus_tag="PKI64_10640"
     CDS             <1383..1766
                     /locus_tag="PKI64_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOE01492.1"
                     /translation="LGRRAMLETFRKNEGYVLFGLDSFWMGVDVRGDALSNVILTRLP
                     FAVPDHPVVAARLQRIKERGGDPFREYSLPEAVLKFRQGFGRLIRSQTDQGIVVVLDS
                     RLLTKWYGRVFKRSLPECPVETFEL"
     gene            1817..>2487
                     /locus_tag="PKI64_10645"
     CDS             1817..>2487
                     /locus_tag="PKI64_10645"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HOE01493.1"
                     /translation="MHSPLANSAATDQSPAAAQPVSRAETWLTRGLMVVAGLLALGLL
                     WSGFCAFTWRKFGLFDYGVYTNMIWNSGHGQLFRCLLDRTYLATHLSFSLALLGPLFH
                     LWDHPFLLWVAQWLMLISGALILWRGALRAHVPALLAWAIVLFLFGYHLNQQVLLSEF
                     HGVSAYLLLVPWLYYCLRFQRRWVWLPWLLTLGLREDAGFIILPLLLYCAVRERWWPG
                     YVYAAL"
BASE COUNT          362 a          696 c          796 g          533 t
ORIGIN      
        1 gactgccaaa agttgagctt gtcacccgta tgatcgctcc gtttccggac tccgctgccg
       61 cggattcgcg cccgttgcgt gaggtggtcg gtgaattttt cgcactgggc ggcgcgctgg
      121 cggatgtcac caccggcgac ttcgagttcg aggcccgccc ccagcaacag cagatggccg
      181 atgccgtggc ggcggccttg gctgatgagc gccacctgat cgtggaagcc ggaaccggcg
      241 tggggaaaag cttggcttac cttgtcccat tgctgctgca cgcccagcag cagggcatcc
      301 gggtgctggt gtccacccac accatcgcac tgcaagaaca gttgctgggc aaagacctgc
      361 ccatgctgca cgagcgcttg ggctttgaat tccgcgcagt gctggtcaag gggcggatga
      421 attatttgtg ccggcggcga ttggcccgcg cgcgcagcca ccagcgcgag ttgttcggcg
      481 atggcgtggc cgaagaactg gagcgtatcc ggacgtgggc cgacaaaacg gaagacggca
      541 gcttgcagga atggcggcat gaaccatccg ccgaggcctg gggcgcggta tgcgccgaac
      601 atggcaattg cctcggtgcc aaatgcccgg agcgcaaacg ctgctttctg ctgcgcgctc
      661 gcgccaagat gcaggatgcc cacttgctgg tggccaacca tcatttgctt ttctccgatt
      721 tggccctgcg gcaggacggc gcggcctttt tgccggatgt gggcgcggta gtgctggatg
      781 aggcccacat ggtggaggat accgccagcg agcaccttgg cctgcgcctc tccacgtatg
      841 gttttgaaca ttggttgcgc cggctattcg tgccagaaag cggcaaaggg cttttgggct
      901 atttgcgcgc cggagccgcg gcccgcgtgg ccaccgaact ttggccggca gtggccgatc
      961 tgttccacga agtgccacgc ttggtcggct tggggccgca cgacggccaa aaggtggtcg
     1021 cccagccctt gcaactggtg accaatgttc cggaattgat gcatcaactc tccacggaat
     1081 tggagattct gggggaagat ttggtcgggc aggacgaaga ctcccgcgcg gaattacgct
     1141 ccttgcgcgg gcagggaatt gccttgagca aaatgctgga ggcttttctg gaccagtcca
     1201 tgccggatca cgtctattgg ctggagcgcg aaggccgccg ccggcagccc gtgctgtatt
     1261 ccgcgcccat cgaagtggcg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnctgggccg gcgcgccatg ctggaaacct tccgcaaaaa cgaaggctat gtgctctttg
     1441 ggctcgatag tttctggatg ggcgtggatg tgcgtggcga cgccctgagc aatgtgattc
     1501 tcacgcgact gccctttgcc gtgccggatc accccgtggt ggcggcgcgg ctgcagcgca
     1561 tcaaggagcg ggggggcgat ccctttcgcg agtattccct gccggaggcc gtgctgaagt
     1621 ttcggcaagg ttttggccgc ctgattcgct cccagaccga ccaaggcatt gtcgtggtgc
     1681 tggattcgcg cctgttgacc aagtggtacg ggcgggtgtt caagcgctcg ctgcccgaat
     1741 gcccggtgga gacgtttgag ctgtaaccgc tgggttttgc cttgctccct ggcgggccat
     1801 tcggcaaggt aattccgtgc attcaccgtt ggccaactct gccgcaacag accagtcgcc
     1861 ggctgccgcc cagccggtgt cgcgcgcaga aacgtggctg acccgcggct tgatggtggt
     1921 ggctgggctg ctggcgctgg gcttgctctg gtccgggttc tgcgccttca cttggcgcaa
     1981 attcggcctg ttcgattatg gcgtttatac gaacatgatc tggaacagcg ggcacggcca
     2041 acttttccgt tgtctgctgg atcgcaccta tttggccacg cacttgtcat tctcgctggc
     2101 attgctgggg ccgctctttc atctctggga ccatccgttt ttgttgtggg tggcgcagtg
     2161 gttgatgctg atcagcggcg cgctgattct ctggcggggc gccctgcgcg cgcacgtgcc
     2221 ggcgcttctg gcttgggcca ttgtgctgtt tctcttcggc taccacctca atcagcaagt
     2281 gctgttgagc gaatttcacg gtgttagcgc ttatctcctg ctggtgccct ggctctatta
     2341 ttgcctgcgg ttccagcggc ggtgggtgtg gctgccgtgg ctgttgacgc tgggcctgcg
     2401 cgaggatgcc gggtttatca tcttgccgct gctgctctat tgcgcggtgc gtgagcgctg
     2461 gtggccgggc tatgtgtatg ccgcgct
//
LOCUS       DAOPNP010000176         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_47182_length_2430_cov_7.702737, whole genome shotgun sequence.
ACCESSION   DAOPNP010000176 DAOPNP010000000
VERSION     DAOPNP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47182_length_2430_cov_7.702737"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1340)
                     /locus_tag="PKI64_10650"
     CDS             complement(171..1340)
                     /locus_tag="PKI64_10650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE01494.1"
                     /translation="MKNIRHYWLPLLLAIALTPAVGCGKKKKTIGAAAPSVPVITVRA
                     QDVAARTFERRLTVQGTLEAKNFANVAARADGNLEEIWVDKGDYVEAGKTKLFQVDAA
                     NRQNSLTIAVQNLAVAEASLAVAQAAAQKAEAEARKATLDKERYERLHRAGNVSDNEY
                     ERAQVQFESADAGLAVARAQVDLAERQVKQADAARAIAKKNVDDARVVAPISGYVSAR
                     SAEPGEQMSVGRVVLRIEDLAVLEAAAFLPAQYYTEVEPGQTKFRLEINGTEAGWHTV
                     TYRSPTINTTLRTFEVKGRIENAGAAAVPGSMADLSLVFESRTGLGVPAPAVLVRNEK
                     NVVFVIKDGLAHQREVQPGLRNDGWLEILSGLNAGEVVVIEGQTQLRDGQPVDVL"
     gene            complement(1342..>2430)
                     /locus_tag="PKI64_10655"
     CDS             complement(1342..>2430)
                     /locus_tag="PKI64_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE01495.1"
                     /translation="AQAGYTSFAKDPKITEREFATAGVDIGMPIFMPSTWFLYAAARQ
                     GYAASGIAAHYVRQGIVLQTTVNYYNVLVQQDMIAALESQVAAAQEMASRARGLAAEG
                     LAANWEGDQAELAAQLRQTELNHARRQMAVLRGELLKGLGLSPTAEITLSGETGDPVR
                     PDGDVADLVLTALATHPELSLADRQVVIKEEQVRQAFCAFLPTLSLFHNGTWTGNELA
                     AHSVNWTTGFGAAWTVFDGFANRARYRAAKVERRQAELARESTFLSIIVGVVAAEAAV
                     HDATEAAAVKQRAYEVTAAKVADYKAKAREGLIPLSDALDAQVLADLAQVALVQSRYQ
                     ERIALANLELAMGLTLVPDLSSATPSEK"
BASE COUNT          400 a          737 c          813 g          480 t
ORIGIN      
        1 gacggcgatg taggggatgt cgacggccgg catgttctcg atggagagct tgcggcacga
       61 gttaaagccc agcgcaatca gcgcaatcag caaacaactg acggcgatgg gccggcgggt
      121 ggaggcattg gctaaaaaca tggcagagtt tccttgcggg tgggtccggg ttagagcacg
      181 tcaaccggct ggccgtcgcg cagttgggtt tggccctcga ttacgaccac ctcgccggcg
      241 ttcaggccgg aaaggatttc gagccagccg tcattccgca ggccgggctg cacttcgcgc
      301 tggtgggcga ggccatcctt gatcacaaac accacgtttt tctcgttgcg gaccagcacc
      361 gcaggtgcgg gtacgccgag gccggtgcgc gactcaaaca ccaggctcaa atccgccatg
      421 ctgcccggca ccgcggcggc gccggcgttt tcaatgcggc ccttgacttc aaaggtgcgc
      481 agcgtggtgt tgatggttgg gctgcggtac gtcaccgtat gccaaccggc ttccgtgccg
      541 ttgatttcca gccggaactt ggtctggccg ggctcaactt cagtgtaata ttgcgccggc
      601 aggaatgcgg cagcttccag tacggcgagg tcttcaatgc gcagcaccac gcggcccacg
      661 gacatttgct cgccgggctc ggcagagcgg gcgctgacat agccggaaat gggcgcaacc
      721 acgcgggcat cgtccacatt ctttttggcg atggcgcgcg cggcatccgc ctgtttgact
      781 tggcgctccg ccaagtccac ttgcgcgcgg gctacggcca agcccgcgtc cgcggactcg
      841 aactgcacct gcgcacgctc gtattcgttg tcgctgacgt tgccggcgcg atgcagccgt
      901 tcatagcgtt ctttgtccag cgtggccttg cgggcttcgg cctccgcctt ttgcgcggcg
      961 gcttgcgcca cggccaggga agcctcagcc accgccaggt tttgtacggc gatggtcagc
     1021 gagttttgcc ggttggccgc atccacctgg aagagcttgg ttttgccggc ttcgacatag
     1081 tcgcccttgt ccacccagat ttcctcaagg ttgccatcgg cacgtgcggc gacgttggca
     1141 aagttcttgg cttccagcgt gccttgcacc gtcaggcggc gctcaaatgt gcgcgccgcg
     1201 acatcctgcg cgcgcaccgt aatgacgggg acgctcggcg cggccgcgcc gatggttttc
     1261 tttttcttgc cgcagcccac ggcgggggtg agcgcaatgg cgaggaggag ggggagccag
     1321 tagtgacgaa tgtttttcat gttatttctc cgagggggtt gcggatgaca aatcagggac
     1381 cagcgtcaga cccatggcca attccagatt ggcgagggcg atgcgttctt gataacggct
     1441 ttgcaccagc gccacttggg cgaggtcggc gaggacctgg gcatcgagcg cgtcgctcaa
     1501 cgggatcagg ccttcgcgcg ccttggcttt gtagtcggcc actttggcgg cagtgacttc
     1561 ataggcgcgc tgtttgacag cggcggcttc ggtggcgtcg tgcaccgcgg cttcggcagc
     1621 caccacgccc acaataatgg ataagaaggt gctttcgcgg gccagttcgg cctgccggcg
     1681 ttccaccttg gcagcgcggt aacgcgcgcg gttggcaaag ccatcgaaca ctgtccacgc
     1741 tgcaccgaag ccggtggtcc agttgacgga atgggccgcc agttcattgc cggtccatgt
     1801 gccattgtgg aacagcgaca gcgtgggcaa aaatgcgcaa aaggcctggc gtacttgctc
     1861 ttccttgatg accacctgtc ggtcggccag cgacagctcc ggatgggtgg ccagcgcggt
     1921 gagcaccagg tcggccacat cgccgtcggg gcggaccggg tcgccggtct cgccggagag
     1981 ggtgatctcg gccgtggggg aaagcccgag gcccttgagc aactccccgc gcaagacagc
     2041 catttggcgg cgggcgtgat tcagttcggt ttggcgcaac tgcgcggcca gttccgcttg
     2101 gtcgccttcc caattggcgg cgagaccttc cgcggccaat ccgcgggcgc ggctggccat
     2161 ttcctgggcg gcggccacct ggctttccag cgcggcgatc atgtcctgct ggacgaggac
     2221 gttgtaatag ttgacggtgg tttgcagcac gatgccctgc cgtacatagt gcgcggcaat
     2281 gccagaggcg gcatagccct ggcgcgccgc ggcatagagg aaccaggtcg agggcatgaa
     2341 aatcggcatg ccaatatcca ccccggccgt tgcgaactcg cgctcggtga ttttggggtc
     2401 cttggcaaag gatgtgtagc ccgcttgcgc
//
LOCUS       DAOPNP010000177         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_47469_length_2420_cov_8.158562, whole genome shotgun sequence.
ACCESSION   DAOPNP010000177 DAOPNP010000000
VERSION     DAOPNP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47469_length_2420_cov_8.158562"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..761
                     /gene="nadD"
                     /locus_tag="PKI64_10660"
     CDS             159..761
                     /gene="nadD"
                     /locus_tag="PKI64_10660"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOE01496.1"
                     /translation="MPRTIGLLGGTFNPVHEGHLSIAREAMRLFGLDGVWFIPCAVPP
                     HKPTHNLASNEDRLAMLQLAIAGESRFAALPIEFERAGKSYTLDTVQMLQLLHPDTAF
                     VFIIGADTLPELHKWHQPLELLARVRFVTLARPGCTPTDEALNLPPPWPERLLADLRT
                     GEPVAVSSREIRAQIAAGQPVDLVPVPVLRYIENHNLYRE"
     gene            891..1226
                     /gene="rsfS"
                     /locus_tag="PKI64_10665"
     CDS             891..1226
                     /gene="rsfS"
                     /locus_tag="PKI64_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOE01497.1"
                     /translation="MRERVKQVRTILDSKKGEHIAILNVAKVSSVTDYMILCSGLNHP
                     HIRALADEVVKQLRLAKPPVAPHRRAGSAESEWIVLDYVDFIVHIFTPGMREYYAMEQ
                     LWKDAATVK"
     gene            1239..2201
                     /locus_tag="PKI64_10670"
     CDS             1239..2201
                     /locus_tag="PKI64_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01498.1"
                     /translation="MKSVTIPADRLDAFTNFLQTAGDVQPFDFITRQEGFIYPTRRQP
                     GTLEMFFFACAHQFGFWLLDGDKWARPMVARLDSQDLKGSDFLWRACTRALPNNPDAW
                     APATLAALADAALAARFHDDHGINPLPMWPEHLALTRAYAAWFAAHDISPAAIVRQVN
                     AAPQPLRALLATLREIPGYAEDPLQKKAMLLAIVLENRPEHFLYVSDPQSAVPIIDYH
                     LQRSALRCGLVRVDDPTLHAKLVARTLLAPEEEAAIRAATYEVVQQLMTRTGLSAAAI
                     DWFFFQNRTRCPETTEPDCAVCPVQAICARETQLFQPILRTTAY"
BASE COUNT          450 a          866 c          634 g          470 t
ORIGIN      
        1 tgcctcgtcc acggcgattc ggaaatctta cgccaatatg tcggccaacc catcacgatc
       61 cgcgggcgcg cctactgggt ggaattgagt acgctgccgg tggtggtggc cggccaagtg
      121 gtcccgctga atcctccggc tgaatccatg gacgactgat gccccgcacg atcggattac
      181 ttggcggcac cttcaacccg gtgcacgagg gccacctgtc catcgcccgc gaggccatgc
      241 gactcttcgg gctggatgga gtctggttca tcccttgcgc cgtgccgccg cacaaaccca
      301 cgcataacct cgccagcaac gaggaccgcc tcgccatgct gcaactggct atcgcgggcg
      361 aatcgcgctt tgcggcgctg cccatcgaat tcgagcgcgc aggcaaatcc tacacgctcg
      421 ataccgttca aatgctccag ttgctgcacc cggacaccgc ctttgtgttc atcattggcg
      481 cggatacgtt gccggaattg cacaagtggc accaaccgct cgaactactg gcgcgcgtgc
      541 gcttcgtgac gctggcgcgc cccggttgca cgcccaccga cgaagcgttg aatctgccac
      601 cgccgtggcc cgaacgcctg ctggccgacc tgcgtaccgg cgagcccgtc gccgtctcct
      661 cgcgcgaaat tcgcgcccaa atcgccgccg gtcagcccgt tgaccttgtc cccgtccccg
      721 tcctccgtta tattgaaaat cacaacctat atcgcgaatg aagaccaaga aaaccccccc
      781 cccccccccc accccccccc cccccccccc aacccccccc cccacccccc cccccccccc
      841 cccccaccaa gccccgcgcg aaatctcgcg ccccctcccc cgccgcgcaa ctgcgcgaac
      901 gcgtcaaaca ggttcgcacc attttggatt ccaaaaaagg cgagcacatc gccatcctga
      961 acgtggccaa ggtgtcgagt gtgacggact acatgatttt gtgttccggt ctcaaccacc
     1021 cgcacattcg cgccttggcc gatgaagtcg taaagcaatt gcgcctggcc aagccgccgg
     1081 tggcccccca ccgccgcgcc ggttccgccg aaagcgaatg gatcgtgctc gattacgtgg
     1141 atttcattgt gcacatcttc acgcccggca tgcgtgaata ttatgccatg gaacaactct
     1201 ggaaggatgc cgccaccgtc aaataaacac ggcgcgccat gaagtccgtc acgattcccg
     1261 ccgaccggct cgacgcgttc accaacttcc tgcaaacggc cggcgatgtg cagccgtttg
     1321 attttatcac ccgacaggaa gggttcatct atcccacccg ccggcagccc ggcacgttgg
     1381 aaatgttttt ctttgcctgc gctcaccagt tcggcttctg gttgttggac ggcgacaaat
     1441 gggcccgccc catggtggcc cggctcgata gccaggattt gaagggctcc gatttcctgt
     1501 ggcgcgcctg cacccgcgcg ttgccaaaca atcccgacgc ctgggcgccc gcaacgctgg
     1561 ccgcgctggc tgatgccgcg ctcgctgcgc ggttccacga tgaccacggc atcaaccccc
     1621 tgcccatgtg gcccgagcat ttggccctga cccgggctta cgccgcgtgg tttgccgcgc
     1681 acgatatatc tcccgcggcg attgtccgcc aagtcaatgc cgccccccaa ccgctgcgcg
     1741 cgctgctggc caccttgcgg gaaatccccg gctatgccga agacccgctt cagaaaaaag
     1801 ccatgctgct ggccatcgtg ctggagaacc gccccgaaca tttcttgtat gtcagcgacc
     1861 cccagtccgc ggtgccgatc attgactacc acctgcaacg cagcgccctc cgctgcggac
     1921 tggtacgcgt ggacgatccg acgctgcacg ccaagctggt cgcacgcaca ttgctcgcgc
     1981 cggaagaaga agctgccatc cgtgctgcca cctacgaggt tgtgcagcaa ttgatgacgc
     2041 gaaccggctt gtccgccgcc gcgattgact ggttcttttt ccaaaaccgc acccgctgcc
     2101 cggaaaccac cgagcctgac tgtgctgtct gccccgtaca ggccatctgt gcccgcgaaa
     2161 cccagctctt ccagccgatt ctacgcacca cagcctatta aaaccggcta tcggccctga
     2221 aattatcccg aaaaatgcat ttgggcatag aggttgcgta atcacccgat gaacattggg
     2281 taatttgggg gtgtcattcg tttggatcaa tggtgacgct ctgttttagg tctcctgcgt
     2341 ttgcgatttt cctcgttatg cttggacggg aggtccaatt gcattgcagt tgaccaggca
     2401 caggggcaac tcgttgatct
//
LOCUS       DAOPNP010000178         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_49746_length_2335_cov_5.524561, whole genome shotgun sequence.
ACCESSION   DAOPNP010000178 DAOPNP010000000
VERSION     DAOPNP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49746_length_2335_cov_5.524561"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PKI64_10675"
     CDS             <1..1142
                     /locus_tag="PKI64_10675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE01499.1"
                     /translation="YVGYPLLIWLLGTLRARKIERAPYSGGISVLIAAHNEAGTLRQK
                     VTSLVELAAQELIREIWLGLDGCTDDTATAVRSLGIVSDGNSDTLSEGLHGEIKRPVV
                     RLLEFAERRGKAAVLNDLMARAGQPVVVMMDARQRIPAGALSTLLEPFADPQVGVVSG
                     ALVYETAEGGAPKGADSYWSYEKQIRLSESRFWAVPGATGALYAIRRELLAPIPAETL
                     VDDVLIPMQAVLRGYRCLFEPKAQVFDRPTSHFGQENLRKRRTLAGIWQTMRLMPKIY
                     SPAANPIWWQWISHKFLRLLTPFLVILALFSLVGLAGEGFLWAKWLLAGALGAMLASA
                     GAYWGAKWFNSRILGLMGAFFGVNWALLQAAGNALTGRFEARWSK"
     gene            1158..>2335
                     /locus_tag="PKI64_10680"
     CDS             1158..>2335
                     /locus_tag="PKI64_10680"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOE01500.1"
                     /translation="MSVPETMKTANDEALEGLVELITFHSEETGFCVLKVKARGFREM
                     VAVVGKIPRINVGEWIKAEGKWHIDRKHGRQFQAAHLEAVVPNSLAGIEKFLGSGLIK
                     GIGPVYAKKLVDAFGMGVLDVIENRSAELERVDGIGPLRRQQIKESWKAQKTIREIMS
                     FLFAHGVSTGRAFRIYKTYGEEAINKVRLDPYCLARDIHGIGFKIADQIAERLGIAKD
                     SILRARAGLTYVLLELSNQGHCAYPRDPLLARAAEMLGIPQGTMEEALRIELENGTLT
                     VGPGEDNETWIYLAALDLAEREVAARLAEISQGAHPCPQINLPAAVEWVQKQLGMELA
                     PGQVAAFQGAFQNKVLVITGGPGVGKTTLVQSLVKVLAAKKLRLMLCAPTGRAAKRMT
                     "
BASE COUNT          474 a          548 c          827 g          486 t
ORIGIN      
        1 tttatgtggg gtatccactg ctgatctggc tcttgggcac attgcgcgcg cgcaaaattg
       61 agcgcgcgcc ctattccggc ggcatttcgg tgctcattgc cgcgcacaat gaagccggca
      121 ccttgcgcca aaaggtgaca tcgttggtcg agctggcggc gcaggagctg attcgggaaa
      181 tctggttggg gttggacggc tgcacagacg acacggcgac ggcggtgcgc agcttgggga
      241 tcgtgagcga tggaaattcg gatacgctca gcgaagggtt gcacggagag atcaagcggc
      301 ctgtggtacg gctgctggag ttcgccgaac ggcggggcaa ggcggcggtg ttgaacgatt
      361 tgatggcgcg cgcgggacag ccggtggtgg tcatgatgga tgcgcggcag cggattcccg
      421 cgggcgcgtt gagtacgctg ttggagccgt ttgccgaccc gcaagtgggc gtggtttccg
      481 gcgcgctggt gtatgagact gctgaaggag gtgcgccaaa gggtgcggac tcctattgga
      541 gctacgagaa gcaaattcgc ctgtctgaaa gccgtttttg ggcggttccc ggtgcaacag
      601 gggcgctata tgccattcgg cgcgagttgt tggccccgat tccggccgaa actttagtgg
      661 atgacgtgtt gatcccgatg caggccgttt tgcggggcta tcgctgcctt tttgagccca
      721 aagcacaggt ttttgaccgc ccaacaagcc attttgggca agaaaatctg cgaaaaaggc
      781 gcacattggc gggaatttgg caaaccatgc gtttgatgcc caaaatttac tcgccggcgg
      841 ccaatccgat ctggtggcag tggatttcgc acaagttttt acgacttttg acgccatttt
      901 tggtgatttt ggcccttttt tcgctggtgg ggcttgctgg agaggggttt ttatgggcaa
      961 aatggctgtt ggcaggggcg ttgggcgcca tgttggccag cgcgggggcc tattggggcg
     1021 caaaatggtt caacagccgg attttggggc tgatgggggc gtttttcggc gtaaattggg
     1081 ccttgttgca ggcggcgggc aatgcgctca cggggcgctt tgaggcacgg tggtcaaaat
     1141 aagatgaaaa cagcgatatg agcgtgccgg aaaccatgaa aaccgccaac gatgaggcct
     1201 tggaaggact ggtggagctg attacgttcc acagcgagga aaccggcttt tgcgtgctga
     1261 aagtgaaggc gcgtggcttc cgcgagatgg tggcggtggt gggcaagatt ccgcggatca
     1321 acgtgggcga atggatcaaa gccgagggca aatggcacat tgaccgcaag catggccggc
     1381 agtttcaagc cgcgcatctg gaagcggtgg tgcccaattc gctagcgggt atcgaaaaat
     1441 ttcttggatc gggattgatc aaaggcatcg ggcccgtgta cgcgaagaag ctggtggatg
     1501 cgtttggcat gggggtgctg gatgtgattg aaaaccggtc cgccgagctg gagcgcgtgg
     1561 acggcatcgg gccactgcgg cggcagcaaa tcaaggaatc gtggaaggca caaaaaacga
     1621 ttcgcgaaat catgtccttt ttgtttgcgc acggggtgag caccgggcgg gcattccgga
     1681 tttacaagac ttatggcgag gaggcgatca acaaggtgcg cctcgatccg tactgcttgg
     1741 cgcgcgacat tcacggcatc ggcttcaaga ttgccgacca aattgcggag cggctgggca
     1801 ttgcaaaaga ctccattttg cgcgcgcggg caggtttgac gtatgtgtta ctggagctat
     1861 cgaaccaagg gcattgtgcg tatccgcgcg atcccttgct ggcccgcgcg gcagagatgc
     1921 tggggattcc gcaaggcacc atggaagagg cgctgcgcat cgaattggaa aacggcacat
     1981 tgacggtggg gccgggcgaa gacaacgaaa catggattta tttggcggcg ttggatttgg
     2041 cggagcgcga agtggccgcg cggttggcgg aaatcagtca aggcgcacat ccgtgccccc
     2101 agatcaattt gccggcagcg gtggagtggg tgcaaaagca acttggcatg gaattggcgc
     2161 cggggcaggt ggcggcgttt cagggcgcat tccaaaacaa ggtgctggtg attaccggcg
     2221 ggccgggcgt gggcaaaacg acgctggtgc aatcgttggt caaggtgttg gcggccaaaa
     2281 aactacggct gatgctctgc gcgccgacgg ggcgggccgc caagcggatg acgga
//
LOCUS       DAOPNP010000179         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_50972_length_2291_cov_3.717800, whole genome shotgun sequence.
ACCESSION   DAOPNP010000179 DAOPNP010000000
VERSION     DAOPNP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50972_length_2291_cov_3.717800"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKI64_10685"
     CDS             complement(<1..503)
                     /locus_tag="PKI64_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOE01501.1"
                     /translation="MKSLVPKIADALLARRWTLATAESCTGGMIGAALTDLSGSSAWY
                     LGGVIAYANSVKMKLLHVLEATLDAHGAVSPETARAMAQGIAQAVGADCAIAVTGIAG
                     PTGGTPEKPVGLVYIATTTPTGINVQAYHFDGSRAAIRSTATRAACQQLLDHLLIRNR
                     FSGARTPA"
     gene            597..1667
                     /locus_tag="PKI64_10690"
     CDS             597..1667
                     /locus_tag="PKI64_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE01502.1"
                     /translation="MKTAIVTGAGGLVGSETVRHFAGQGFRVIGVDNNLREYFFGPEA
                     STRWAVQELQRAFPESFQAVDADIRDFEAVRKVFEDAGNIDLVVHTAAQPSHDWAARE
                     PFTDFSVNANGTLNLLEATRQLAPEAVFIFTSTNKVYGDTPNRLPLEEQATRWECVDG
                     PYAEKGIDESMSIDHSTHSLFGASKVAADILTQEYGRYFGLKTGTFRGGCLTGPRHSG
                     AELHGFLAYLMKCAATGREYRIFGYRGKQVRDNIHSADLVASFEHFYHAPRPGEVYNM
                     GGSRFSHCSMQEAVQLCEEITGRNMKTTYVETNRIGDHIWWVSDVSKFQSHYPDWKLT
                     KNVRDILEEILEYNLPRWKKEA"
     gene            1664..2269
                     /locus_tag="PKI64_10695"
     CDS             1664..2269
                     /locus_tag="PKI64_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE01503.1"
                     /translation="MSRRIMRAGSFIFCDSQLLVRADAPFTPAEKDALDLFASPRTVA
                     ESFVMRPEGVTVYALAGEPGAAPDGYDWVRLRMMLGLGLPHTAAALRALGLLNWRESH
                     RFCGCCGGPLEEHAVEVARFCPACGHLEYPGIAPAVIVRVEKDGKILLARHVQRIPHL
                     WTCLAGYVELGESLEDCVRREVREEAGIQYIECFHFRSWDL"
BASE COUNT          412 a          586 c          787 g          506 t
ORIGIN      
        1 gcgggtgtcc gtgcccctga aaagcgattt cttatcaata gatggtcgag aagttgttgg
       61 caggcggcgc gtgtggcggt tgagcggatg gcggcgcgtg agccgtcgaa gtggtatgcc
      121 tggacgttga ttcccgtcgg cgtggtcgtt gcgatatata cgaggcctac tggtttttcc
      181 ggcgtgccgc cggtggggcc ggcgatgccc gtgacggcga tggcgcaatc ggcgccgacg
      241 gcttgggcaa ttccctgcgc catggctcgc gcggtttcgg ggctgactgc tccgtgggca
      301 tccaaggttg cttcgaggac gtgaaggagc ttcatcttga ccgaattggc gtaggcgatt
      361 actccgccca gataccaggc ggagctgccg gagaggtccg tcagggcggc gccaatcatc
      421 ccgccggtgc aggattccgc cgtggccagc gtccagcgtc gggcgagaag ggcgtcggcg
      481 attttgggga caaggctttt catgccgcca tcctacgcct gccatgccgt tttgcaaagt
      541 tttccatgac atttttccat gacatttacg gcgctatttg agaaaatgcc cggataatga
      601 aaacagctat tgtgacagga gccggcggat tggtggggtc ggaaacggtg cggcattttg
      661 ccggtcaggg gtttcgcgtg attggggtgg ataacaattt gcgggagtat ttctttgggc
      721 cggaggcgtc cacgcggtgg gcggttcagg agcttcagcg ggcttttcct gagtcgtttc
      781 aggcggtgga tgcggatatt cgcgacttcg aggcggtgcg caaggtgttt gaggatgcgg
      841 gcaacattga tttggtggtg catacggcgg cgcaaccttc gcacgattgg gcggcgcgtg
      901 agccgttcac ggatttcagt gtgaatgcca acggcacgtt gaatctgctg gaggcgacgc
      961 ggcaactggc gccggaggcc gtctttattt tcacatccac gaacaaggtt tatggcgaca
     1021 cgcccaatcg cctgccgctc gaagagcagg ccacccggtg ggagtgcgtg gacggcccct
     1081 atgccgaaaa gggcattgat gagagcatga gcattgacca ttccacgcat tcgctgtttg
     1141 gcgcgtccaa ggtggcggcg gatattctga cgcaggaata tggccgctat ttcgggctga
     1201 aaaccggcac gtttcgcggg ggatgtctga ccgggccgag gcattccggc gcggagttgc
     1261 acggtttttt ggcgtatctg atgaagtgcg ccgccacggg ccgcgaatac cggattttcg
     1321 gttatcgggg caagcaggtg cgcgacaaca ttcacagcgc cgatttggtg gcttcctttg
     1381 agcatttcta tcatgcgccg cgtccgggcg aagtgtataa catgggcggc agccgcttca
     1441 gccattgctc catgcaggag gccgtgcaac tgtgcgagga aatcaccgga cgcaacatga
     1501 aaaccaccta cgtggaaacc aatcgcatcg gcgaccatat ctggtgggtg agcgatgtca
     1561 gcaagtttca gagccattat ccggattgga agctgacgaa aaacgtccgc gatattcttg
     1621 aagaaattct ggagtacaat ctcccgcgct ggaagaaaga ggcttgagcc gccggatcat
     1681 gcgcgcgggc agcttcatat tttgcgacag ccaattactg gtgcgcgccg acgcgccgtt
     1741 cacgccagcc gagaaggacg ctctggactt atttgcgtcg ccgcgaaccg tggcggagtc
     1801 gtttgtgatg cgcccggagg gcgtcacggt ctatgcgctt gcgggcgagc cgggggccgc
     1861 gcccgatggc tacgactggg tgcgattgcg catgatgctg gggctgggtc ttccacacac
     1921 ggcggcggcg ctgcgcgccc ttgggctgct gaattggcgc gagtctcatc gtttctgcgg
     1981 ctgttgcggg gggccattgg aggagcacgc ggttgaagtt gcgcggtttt gtcccgcttg
     2041 cggccatctg gagtatcccg gcatcgcgcc ggcggtgatt gtgcgcgtgg aaaaggacgg
     2101 gaagattctg ctggcgcgcc atgtgcagcg gattcctcat ttgtggacgt gtctggccgg
     2161 gtatgtcgag ctgggtgaat ctctcgaaga ctgcgtgcgg cgcgaggtgc gcgaagaagc
     2221 gggaatccaa tatatagagt gttttcattt tcgtagttgg gacctgtaac cttccagcga
     2281 tataaggccg c
//
LOCUS       DAOPNP010000180         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_52280_length_2247_cov_10.595347, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000180 DAOPNP010000000
VERSION     DAOPNP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52280_length_2247_cov_10.595347"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKI64_10700"
     CDS             <1..784
                     /locus_tag="PKI64_10700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOE01504.1"
                     /translation="GKLVVLTFHRVRPAGEQPPPRPMRNLEVDADDFRRLLCWLCARY
                     QPVALGDWLDFAAAPPAPSFAITFDDGWADNADHAWPILRELQIPATIFLATGAVAER
                     TPFWWQTTGLSDAEIERRKTQPPVPAGNAAPSADFLTWAQINAMGAEGLITFGAHGHR
                     HLLLDQVSAADALADIRQCWSLLRQNVRAGLLPVLAWPNGNARDDLTPALQELGLRAA
                     FSAARGAVATPAAARWRLPRNNVDRQLSRQPELWPWLMMRAK"
     gene            760..1032
                     /locus_tag="PKI64_10705"
     CDS             760..1032
                     /locus_tag="PKI64_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01505.1"
                     /translation="MADDARQIALLAFFHSVEKNLSRGGGIFHSVEKFPKVVPLRGKV
                     APKLFHCVEKFLRVFPLRGKIGASFSTVWKNLGSLFHSVESPARAG"
     gene            1072..2151
                     /locus_tag="PKI64_10710"
     CDS             1072..2151
                     /locus_tag="PKI64_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01506.1"
                     /translation="MDEVKWAWVLLVAATLALLIRLAMWFLPEPGWRSAKVTQAAEVL
                     SSETGVVEEAAAPFIATPEPEVAAAPAVAENIAREPAPEPAVAEPAPAAAVPAVAETA
                     PVAPPSPPPVYNLRLTRWGMSRADVEAAELNEPIRENATTLVYATTTLELPCVLTYYF
                     EEGRLTRARLSFADPDGVDIPPLNAAQAQRRFLFLREQLRSRYGQPVQRTFHQPRDVS
                     GLQRTAEQQEELAQQYDVEIAEAEQRLQKQRDVLTKRFRRWSNAKEMVERGLAPYERD
                     IRDLRQWKKDALARADQSRRDIQAHRSADTAQPLIGVMLARWPYAREAQNIELRLDCR
                     GSSPRLDVRYEAAPKASGWGYFNDL"
BASE COUNT          400 a          721 c          737 g          389 t
ORIGIN      
        1 tggcaagctg gtggtgctga ccttccaccg cgtgcgcccc gccggtgagc agccgccccc
       61 gcgccccatg cgcaacctcg aagtggatgc cgacgacttc cgccgcctgc tgtgctggct
      121 gtgtgcccgc taccagcccg tggccttggg cgattggctg gacttcgccg ccgctccacc
      181 cgcgccctcg ttcgccatca ccttcgatga cggctgggcc gacaacgccg accacgcgtg
      241 gcccatcctg cgcgagctac aaatccccgc caccattttt ctggccacgg gcgcggtggc
      301 cgaacgcacc ccgttttggt ggcaaaccac cgggctcagc gatgcggaaa ttgaacggcg
      361 caaaacgcag ccccccgtgc ctgctggcaa cgccgcgccc agcgcggatt ttttgacgtg
      421 ggcgcaaatc aacgcgatgg gcgcggaggg actcatcacc tttggcgcgc acggccaccg
      481 gcacctgctg ctggatcagg tatcggccgc cgatgcgctg gcggatatcc gccaatgttg
      541 gagcctgctg cgccagaacg tccgcgccgg gttgctgccc gtactggcgt ggcccaacgg
      601 taacgcgcgc gacgatctca cccccgcgct gcaagagctg ggcctgcgcg ccgcgttcag
      661 cgccgcccgc ggcgcagttg ccacccctgc cgccgcgcgc tggcggctgc cgcgcaacaa
      721 tgtggaccgc caattatccc gccaaccgga gctttggcca tggctgatga tgcgcgccaa
      781 atagccctgc tcgcattttt ccacagcgtg gaaaaaaatt tatcccgcgg gggcgggatt
      841 ttccacagtg tggaaaaatt ccccaaagtt gttccattgc gtggaaaagt ggccccaaag
      901 ttgttccatt gtgtggaaaa attcctgaga gtttttccat tgcgtggaaa aatcggcgca
      961 agtttttcca cagtgtggaa aaatctggga tcattgttcc acagtgtgga aagtcccgcc
     1021 cgcgcgggat gaaaatattt ccatagtgtg gaacgctgga gtggaggagc gatggacgag
     1081 gtaaaatggg catgggtgct gctggtggca gcgaccttgg cgctgttgat ccggctggcc
     1141 atgtggtttt tgccggagcc gggctggcgc tcggccaagg tgacgcaggc ggcggaagtg
     1201 ttgtcctcgg aaacgggcgt ggtggaagaa gccgccgcgc cgtttatcgc cacgccggag
     1261 ccagaagttg ccgcagcgcc ggcggtggcg gaaaacatcg cgcgtgagcc cgcgccggaa
     1321 ccggcggttg ccgagcccgc gcccgccgcg gctgttcccg cggtggctga aaccgcgccg
     1381 gtcgcgccgc caagtccgcc gccggtctat aacttgcggc tcacccgttg ggggatgtcg
     1441 cgcgcggatg tagaggcggc ggagctgaat gaaccgatcc gcgagaacgc caccacgctg
     1501 gtctatgcca ccaccacgct ggagctgccg tgcgtgctga cgtactattt tgaggagggg
     1561 cggctgacgc gcgcgcggct ttcgtttgcc gatcccgatg gcgtggatat tccgccgctg
     1621 aacgcggcgc aggcgcagcg tcggttttta tttttgcgcg agcagttgcg cagccgctac
     1681 ggccagcccg tacagcgcac cttccaccag ccgcgcgatg tctccggctt gcagcgcacc
     1741 gcggaacagc aggaggagct ggcgcagcag tacgacgtgg agattgccga ggccgagcag
     1801 cgcttgcaaa aacagcgcga tgtgctgacc aagcgcttcc gccgttggag caacgccaag
     1861 gaaatggtgg agcgcggcct tgcgccgtat gagcgcgaca tccgcgattt gcgtcagtgg
     1921 aaaaaagacg cgctggcccg cgccgaccaa tcgcggcgcg acatccaggc ccaccgcagc
     1981 gcggataccg cgcagccact gattggcgtg atgctggcgc gctggcccta tgcgcgcgaa
     2041 gcgcagaaca tcgagctgcg gctggattgt cgcggcagct caccgcgact tgacgtgcgc
     2101 tacgaagccg cgcccaaagc aagcgggtgg ggctacttca acgacctgtg acgcacgggg
     2161 cgaacgaaac ctttccgccg agaaaacggc tggaaggtct gcgaccattc cagccctaca
     2221 atcgaaccag atgcttttgt gggccgg
//
LOCUS       DAOPNP010000181         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_52935_length_2227_cov_11.494936, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000181 DAOPNP010000000
VERSION     DAOPNP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52935_length_2227_cov_11.494936"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="cas4"
                     /locus_tag="PKI64_10715"
     CDS             <1..504
                     /gene="cas4"
                     /locus_tag="PKI64_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOE01507.1"
                     /translation="NRFTAEGRLAHEKVHSAGQEVRDGVRTVRDLTLRSLRLGLVGKT
                     DVVEFRDGVPYPVEHKRGRDKANDCDRVQLCAQALCLEEMLGITVPEGAIFYGQPRRR
                     TVVPLDATLRQKTEEAAARLHALFDARVTPPAVYDKHKCEPCSLLNECQPRAGGNVTR
                     YLQEALQ"
     gene            501..1529
                     /gene="cas1c"
                     /locus_tag="PKI64_10720"
     CDS             501..1529
                     /gene="cas1c"
                     /locus_tag="PKI64_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOE01508.1"
                     /translation="MKQLLNTLYITTQGSYLRKEGETIVVEAEQEVRARLPIHNIGGL
                     VCFGNILCSPFLLGHCGENGVAVSFLTEYGKFLARVAGPQSGNVLLRREQYRRADNLD
                     QSAELARMFVFGKVNNCRTVLQRHLRDHPDTPDNGLVEALVVRLQQMLTRLQTPLALD
                     EVRGLEGDAARAYFGVFNVLLNAASGFEFHERSRRPPLDPINALLSFLYTLLAHDCRG
                     ACEAVGLDPQVGFLHRDRPGRPGLALDLMEEFRPFLVDRLVFTLINRQQITPKDFETS
                     ETGAVLLKEKPRKLVLQSWQERKLDSLTHPFLNEKMAVGLLPHAQAQLLARHLRGDTD
                     LYPPFLWK"
     gene            1533..1823
                     /gene="cas2"
                     /locus_tag="PKI64_10725"
     CDS             1533..1823
                     /gene="cas2"
                     /locus_tag="PKI64_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOE01509.1"
                     /translation="MMVLVSYDVSTVDKRGQARLRKVAKVCEDYGIRVQNSVFECVVD
                     PALWVAFKAQLEKIYDPEQDSLRFYFLGSNWGKRIEHLGKTKGLDVTEPLIF"
BASE COUNT          448 a          638 c          682 g          459 t
ORIGIN      
        1 aatcgcttca cggctgaagg gcggctggcg cacgaaaaag tgcatagcgc aggccaggag
       61 gttcgcgacg gtgtgcgcac ggtccgggat ttgacgttgc gctcgttgcg gctggggttg
      121 gtgggcaaga cggatgtggt ggagtttcgt gatggcgtgc cctatcccgt ggagcacaaa
      181 cgcgggcgcg acaaggcgaa cgactgcgac cgggtgcagc tttgcgccca agctttgtgc
      241 ttggaagaaa tgttgggcat taccgtaccc gagggggcca ttttttatgg ccagccccgc
      301 cgccgcactg tcgtgccgtt ggatgccacc ctgcgccaaa aaacagagga ggccgccgcc
      361 cggctccatg ctctatttga tgcacgcgtg actccgcccg cggtctatga caaacacaag
      421 tgcgagccct gctctttgtt gaatgaatgc cagccccggg ccggcggcaa tgtcacccgc
      481 tatcttcagg aggcgctgca atgaagcaat tgctgaacac gctctacatc accacacagg
      541 gcagctatct gcgcaaggaa ggcgaaacca tcgtggtgga ggccgaacag gaagtgcgcg
      601 ctcgtttgcc catccacaac atcggcggct tggtgtgttt tggcaatatc ctgtgcagtc
      661 cgtttttgct cgggcattgc ggcgaaaatg gcgtggcggt cagttttttg acggagtacg
      721 gcaagtttct ggcccgggtg gcgggcccgc aatccggcaa cgtcctgctg cgtcgtgaac
      781 aataccgccg ggcggacaac ctagatcaat cggcggaatt ggcccggatg tttgtcttcg
      841 gcaaagtcaa caactgccgg acagtgttgc agcgccattt gcgtgaccat cccgataccc
      901 cggacaatgg tctggtggaa gcgctggttg tccgcttgca gcagatgttg acgcggttgc
      961 aaacgccact ggcgctggat gaagtgcgcg gactggaagg cgatgccgcc cgtgcctatt
     1021 ttggcgtgtt caacgtgctg ttgaatgccg ccagtggttt tgaatttcac gaacgaagcc
     1081 gccggccgcc gctggacccc atcaacgccc tgctgtcctt tctatacacc ctgctggctc
     1141 acgattgccg tggcgcgtgc gaagccgtcg gcttggaccc ccaagtgggt tttctgcatc
     1201 gcgaccgccc cggcagaccc ggactggcgc tggacttgat ggaagaattc cggcccttcc
     1261 tggtggaccg ccttgttttc acgctcatca accgccagca aattaccccc aaggattttg
     1321 aaacctcgga aaccggcgcc gttctgctca aagaaaaacc gcgcaaattg gttttgcaat
     1381 cgtggcagga gcgcaaattg gacagcctga ctcatccttt cctcaatgaa aaaatggcgg
     1441 tgggtttatt gccgcacgcc caagcccaac tgctggcccg ccatctgcgg ggcgacaccg
     1501 atctctaccc gcccttcctg tggaaatagg ccatgatggt gctcgtcagt tatgatgttt
     1561 ccacggtgga caagcggggg caggcccggt tgcgcaaagt ggccaaggtg tgcgaggact
     1621 acggcatccg agtgcaaaat tccgtctttg aatgcgtggt ggacccggcc ctgtgggtgg
     1681 ccttcaaggc ccaattggaa aaaatctacg accccgaaca agacagcctg cgtttttatt
     1741 ttcttggttc caactgggga aaacgaatcg aacatctcgg caaaacaaaa gggttggatg
     1801 tcaccgaacc gctcattttt taatgtgcgc gaaccccaag cgcccatcaa aaccccggga
     1861 ggttcgcgcc gacgacaacc atctggtgga gcaagagttg tggctccaag gtgctggatt
     1921 ggctctttga caattgcata aagaacatcg ttcgcgcggg gtggtggatt gcatcacacc
     1981 gcagaatgac ttacaaaaag atggtcgcgc cccgcgcggg gcgcgtggat tgaaactatc
     2041 tgtagaaaca ctctccgccc caatatcctc agtcgcgccc cgcgcggggc gcgtggattg
     2101 aaacaccgat tttgcgaaac gtggcgcggg ccgtttcggt cgcgccccgc gcggggcgcg
     2161 tggattgaaa ccggtgcccg gtggcgcgac gtataccggt gactggtcgc gccccgcgcg
     2221 gggcgcg
//
LOCUS       DAOPNP010000182         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_52971_length_2226_cov_7.815292, whole genome shotgun sequence.
ACCESSION   DAOPNP010000182 DAOPNP010000000
VERSION     DAOPNP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52971_length_2226_cov_7.815292"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1142
                     /locus_tag="PKI64_10730"
     CDS             63..1142
                     /locus_tag="PKI64_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE01510.1"
                     /translation="MLLPTLIFDAAINLDARELWRNIVPISYLAIPGVLVTAAVVGLL
                     VAKFTPLGLNAAMLFGALISTTDTAAVIALFNELNAPKRLAMLMDGESLFNDATAIVL
                     FDIIAAIVAGSLLLANPAEAWTLNTLAGIPGKFLLVFFGGGLVGAAVGWLLMQVIRFS
                     HHDPLLEVGLTTVVAYAAFILANHYCKFSGVMAACGAGLVVNYYGRGILAENMLGTIR
                     KFWAFAAFMANSLIFLLLGLTESFLIQDLGRLRHVAGAVGVAILAVLLARALILGLTA
                     ACYHPFAKPEKKLSWPVLFVMWWGGLRGALPIALAVSIGPALVPPAERTLVIQLTLGI
                     ILFTMLIQGTTLKHFLRRYGLHDAA"
     gene            1209..2156
                     /locus_tag="PKI64_10735"
     CDS             1209..2156
                     /locus_tag="PKI64_10735"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOE01511.1"
                     /translation="MRAWLKRLYHETDWGSRALFPLVQARAWLQGGWRSDRSVIAATF
                     RRTFGYDLDWANPRTLNEKMNWLKLHYRPPLQTVVADKYAVRDFVRERVGERYLIPLL
                     RVYDRAEDIRLAELPSAFVLKVNHGSGQNWIVRDKIREDERQIIRVFRQWLRTSHYLA
                     SREWPYKNIRPRIVAEELLRDESGGIPPDFKFHCFAGKVATIQVDLDRETAHRRNFYD
                     RDWALQPFIWTEWEGDTPSWPNGRAVSRPIQLPEMIQVAETLAVGFPYVRIDLFCCQN
                     RVYFGEVTFYHGGGFERFAPAEYDLHFGDLLRLPPATKA"
BASE COUNT          386 a          656 c          681 g          503 t
ORIGIN      
        1 acagtgaagc acttattgtg tcataatccc atccaaacgc attttccggg tttattctgt
       61 atattttatt gccgacgctg atcttcgatg ccgcgattaa tctcgacgcg cgcgagctgt
      121 ggcgcaacat cgtgcctatt tcctacctgg cgattccggg ggttctcgtc accgccgccg
      181 tggtcgggct gctggttgcc aaattcacgc cgctggggct caatgcggcg atgttgttcg
      241 gtgcgctgat ttccaccacg gacacggccg ccgtcattgc gctcttcaac gaactgaacg
      301 cgcccaagcg cctggcgatg ttgatggacg gcgaaagcct tttcaacgat gccacagcca
      361 tcgtgctgtt cgatattatt gccgcgattg tcgcgggcag cctgctgctg gccaatccgg
      421 cggaagcgtg gaccctcaac acgctggccg gcattccggg caaattcctg ctggtatttt
      481 ttggcggcgg actggttggc gcggccgtcg gctggctgct gatgcaggtc atccgcttct
      541 cgcaccacga ccccctgctg gaagtgggcc tgacgaccgt ggtggcttat gcggcgttca
      601 tcctggccaa ccattactgc aaattttccg gcgtgatggc cgcgtgtggc gcagggctcg
      661 tggtcaatta ttatggccgg ggcatcctgg ccgaaaacat gctgggcacc atccgcaagt
      721 tttgggcgtt cgcggcattc atggccaaca gcctcatttt cctgctgctg ggcttgacgg
      781 aatctttcct gattcaagac ttggggcgct tgcgccacgt ggcgggggcg gtgggggtgg
      841 ccattttggc cgtactcttg gcgcgtgcgc tcatcctggg gctgaccgcc gcgtgctacc
      901 atccttttgc caaaccagaa aagaagctgt cgtggccggt gctgttcgtg atgtggtggg
      961 gcgggctgcg cggcgcattg ccgattgcgt tggctgtttc catcgggccc gcgctggtgc
     1021 cgccggcgga gcggacgctg gtcatccaac tgaccctcgg cattattctc ttcaccatgc
     1081 tgatccaggg caccacgctc aagcacttcc tgcgtcggta cggcttgcac gacgccgcct
     1141 agccggtgat ggctaccggg acgtagcggg gacttgaact ccgggtgggg tgggggcagt
     1201 atggcgtcat gagggcatgg ctcaagcggc tatatcacga aaccgactgg ggaagccggg
     1261 cgcttttccc cttggtgcag gctcgtgcct ggctgcaagg gggatggcgg tcggaccgga
     1321 gcgtcatcgc cgctacgttt cgccggacct tcggctatga cctcgattgg gcgaacccgc
     1381 gcacactcaa cgaaaaaatg aactggttga agctgcatta ccgtccgcca ctccaaaccg
     1441 tcgtggcgga taaatatgcg gtgcgcgact tcgtgcgcga gcgggtgggg gagcgctacc
     1501 tcattcccct gttgcgcgtt tatgaccggg cggaagatat ccggctggcg gagttgccca
     1561 gcgcgtttgt cctcaaagtg aatcatggca gcggccaaaa ctggatcgtg cgcgataaaa
     1621 tccgcgaaga tgagcgccaa atcatccggg tgttccgtca atggctgcgc accagccact
     1681 atctggcctc ccgcgagtgg ccctacaaaa atatccggcc ccggatcgtc gccgaagagt
     1741 tgttgcggga tgaatccggc ggcatccccc ccgatttcaa gtttcactgt tttgccggca
     1801 aagtggccac catccaggtg gatttggacc gggaaaccgc ccaccggcgg aacttttacg
     1861 accgggattg ggccctccag ccgtttattt ggaccgagtg ggagggcgat actccctctt
     1921 ggcccaacgg ccgcgctgtg tcccgcccga tccaattgcc cgaaatgatc caagtggccg
     1981 aaaccctggc ggttggcttt ccttacgtgc gcattgacct cttttgctgc caaaaccggg
     2041 tctatttcgg cgaagtcacc ttctatcatg gcggtggctt tgaacgattt gctcccgcgg
     2101 aatacgacct ccacttcggc gatttgctcc gtctgccacc ggcaaccaag gcctgatagg
     2161 gggcgtattc tgcgcccgtt gcgtcaaatg aaaatgacac cgaaaattac gctgcagggg
     2221 gagatt
//
LOCUS       DAOPNP010000183         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_53129_length_2221_cov_7.682364, whole genome shotgun sequence.
ACCESSION   DAOPNP010000183 DAOPNP010000000
VERSION     DAOPNP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53129_length_2221_cov_7.682364"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..192
                     /locus_tag="PKI64_10740"
     CDS             25..192
                     /locus_tag="PKI64_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01512.1"
                     /translation="MMEAFLDVSDMNRRLARQRRHLAAVRALWVVAYSAAAFGVGLLL
                     GMVLAGIGAGW"
     gene            601..780
                     /locus_tag="PKI64_10745"
     CDS             601..780
                     /locus_tag="PKI64_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01513.1"
                     /translation="MMVFHCAQGAAAGWVESGGLAAEQKRAALRRVLEPLVAELVQQA
                     LQRAGARAGQEERGE"
     gene            1024..1950
                     /locus_tag="PKI64_10750"
     CDS             1024..1950
                     /locus_tag="PKI64_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01514.1"
                     /translation="MSGGLTNYDRASLRLADAISRHDFSEREWRVLEFLRKASFGAGR
                     ATAYVPALKFFCAATGISRGNMSEILRRLKACQVIREEQDGCYGFMLLPPPGDPCANW
                     RVPVRMQDVDVIRQIELLSPPPGLPDMLREGFIEASGQVKQGDARQVYIGVPESGTQM
                     HRSRFGNDLESSMVAGKSCAVPDSGTTHVQCNNEQCNNASSKKHYMDIVSCAAPDSGT
                     GTRRAERRELLDKLIKVGAFDPEKNDESKRFWFWMVKSQPAIVATLLGELKYACAMRR
                     IYSPGGWMRDLWVRWKRPSDNGGSRLVQHTTR"
BASE COUNT          429 a          414 c          874 g          504 t
ORIGIN      
        1 agcagttgat ggaaaggatg tgaaatgatg gaagcgtttt tggatgtgtc ggatatgaat
       61 cggcggctgg cgcggcagcg gcggcacctg gcggcggtgc gggcgttgtg ggtggtggcg
      121 tattcggcgg cggcgtttgg ggtggggttg ctgctcggca tggtgctggc cgggattgga
      181 gctgggtggt gacggcgcgg gcgcgggggt tgggggagga tgcgcgagtg gccaggcggg
      241 cgcggacgtt gaataagacg ccgtggctaa accggcgggc ggtgcgggag gagctgctgc
      301 ggctggcgaa ggagctgcgg ccgtttcatt cttatactcg ggtgtcggag gagacgctgg
      361 tgcagctgag ttggcatttt agacaggcga tgatttggca tgtgaagtcc atgcccagca
      421 agggcaggac gatttgaggc gatgggtggg aatggtcaac cgcggttgtt aagccggaag
      481 gcggcggcgg cgcggtgtgg ggttacggtg cggacgtttg atcggtgggt gcggagcggg
      541 aggctgacgg catatcggtt tggtgggcgg ttgcggtttg cggaggaaga actgggtggc
      601 gtgatggtgt ttcactgcgc gcaaggtgcg gcggctggct gggtggaatc tggcgggctg
      661 gcggcggagc agaagcgggc ggcgctgcgg cgggttttgg agccgctggt ggcggagttg
      721 gtgcaacagg ctttgcagcg cgcgggcgcg cgggccggcc aggaggagcg tggtgagtga
      781 ggtgatgtat tattgtcgcg cgtgtgggct ggattcgttt ttgcggcgcg ggccgcacaa
      841 gaaggctccg ggatcgggga atccggtttg tccgggtgat tttgttgagc agcgggtgga
      901 gagctgcttt tgctgcaagt ggcggcggaa gaaggcgtgt cctgatcctg gggtgatcgg
      961 gcgcagtccg tggatgggct gcggggcttg ggtgctggag gagttttaaa agagaaagtg
     1021 actatgagcg gaggattgac aaattacgat cgggctagtc tgaggctggc ggatgctatt
     1081 tcgcggcacg atttttcgga gcgggaatgg cgggtgctgg agtttttgcg gaaggcgtct
     1141 ttcggcgctg gccgggcgac tgcgtatgtt ccggcgttga aatttttttg cgcggcgact
     1201 ggaatttcgc gcgggaacat gtcggagatt ttgcggcggc tgaaggcatg ccaggttatt
     1261 cgtgaggagc aggatgggtg ctatggattt atgctgctgc ctccgccagg cgatccgtgc
     1321 gcgaattggc gggtgccagt gcggatgcag gatgtggatg tgatcaggca gatagagctg
     1381 ctatcgccgc cgccagggct gcctgatatg ttgcgagaag gatttattga ggcgtccggc
     1441 caggtcaaac aaggcgacgc gcggcaggtt tatattggtg ttcccgaatc tggtacacaa
     1501 atgcatcgtt cccgattcgg gaacgactta gaatcttcaa tggtagcagg caaatcgtgc
     1561 gctgttcccg attctggtac gacacatgta caatgtaaca atgaacaatg taataatgct
     1621 tcttcgaaga agcattatat ggacattgta tcatgtgctg ctcctgattc gggaacagga
     1681 acgcgaagag cagagcgtcg tgagctgttg gataaattga taaaagtcgg ggcattcgat
     1741 ccagaaaaga atgacgaaag taaacgattc tggttttgga tggtcaaatc acaacctgcc
     1801 attgtcgcca cgcttttggg tgaactaaaa tatgcatgcg caatgcgccg aatatactca
     1861 ccaggtgggt ggatgaggga tttgtgggtg aggtggaaga ggccaagtga taatggtggg
     1921 tcaaggctag ttcaacacac cacccgatag gagtcttcta aacccagatg atatcgaaca
     1981 ggttaccaag cgcggattgg gggggggtga gagtggggcg gaaaagtgat ttcagtttca
     2041 cgatttggtt ttacgaattt ttggggctgg gcgtgtgggt ggcgctgtgg gcgcgatggg
     2101 atggggcggg gggtgggaga tgattttcgg ccggttggcg gccggattgg caaagtgaac
     2161 ggataactga taacgaaagg aaatgagatg aacgtgaggt tagtgttggt ggaggagatg
     2221 c
//
LOCUS       DAOPNP010000184         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_57873_length_2085_cov_7.736946, whole genome shotgun sequence.
ACCESSION   DAOPNP010000184 DAOPNP010000000
VERSION     DAOPNP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57873_length_2085_cov_7.736946"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKI64_10755"
     CDS             <1..420
                     /locus_tag="PKI64_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01515.1"
                     /translation="LRAFLRTPRTFLHFPPPTPPHEVALAALAALRAKDWIAARRFEP
                     FYVELSGIVRHYLEGRFGLRAPERTTEEFIREAAAARQLAADHRELVADFLTQSDLVK
                     FARHAPDESAMRGALESAERLVHETTPAAPAANGGAP"
     gene            417..1400
                     /locus_tag="PKI64_10760"
     CDS             417..1400
                     /locus_tag="PKI64_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE01516.1"
                     /translation="MIHWHYPLVWLLALLVPGAVWLRYARRRRAPLAFSDVAALRDLP
                     RSPWLALRWLPPALLAAGLLLLIAAAARPQKGLTESRVDTEGVDIVLVVDTSTSMRAD
                     DFSTALKRMDRLDAAKSVLTQFIKARPDDRMGIVAFAAMPYTIAPLTTDHAWLLLQLD
                     RLQTGMLEDATAIGDAIASGVNRLRDSAAKSKLVILLTDGINNAGRMSPQDAAQAAAA
                     LGIKVYTIGAGADRPRGQGIFGFMQAGNEIDEAALKKIAEITHARYFRATDLKSLEET
                     YQAIDQMEKTKIELNQYTRYEEKFQPFLVLGLLALALETLLGYTRLGGLPC"
     gene            1394..>2085
                     /locus_tag="PKI64_10765"
     CDS             1394..>2085
                     /locus_tag="PKI64_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE01517.1"
                     /translation="MLKWGHGEMLFALLLLAPVAALLLALLKRRRRAIHRLVAAEALP
                     TLAPAWNPARARSRLALRLAALVFLIVALARPQWGFHWEEVRRKGLDLMIVLDTSRSM
                     LAADIKPSRLQQAKWGVRDLLRNLRGDRVGLVPFAGSSIVQCPLTVDYAAFTMTLDDL
                     YAGIIPRGGTAIAQALQTAMNAFPAESAADRVILLITDGEDHEGDPLALLPELKAKNI
                     RVYTIGIGTLEG"
BASE COUNT          323 a          721 c          674 g          367 t
ORIGIN      
        1 ttgcgtgcgt ttttgcgcac gccgcgcacg tttctccatt ttccgccgcc caccccgccg
       61 cacgaagtcg ccctcgcggc gctggccgcg ctgcgcgcca aggactggat cgccgcgcgg
      121 cgcttcgagc cattctatgt ggagctctcc ggcattgtgc ggcattacct ggaggggcgc
      181 ttcggcctgc gcgcgccgga gcgcaccacg gaggagttta tccgcgaggc cgccgccgcc
      241 cgccaactgg ccgccgacca tcgcgagctg gtggccgact tcctcacgca aagcgacttg
      301 gtcaagtttg cccgtcacgc gccggatgaa tccgccatgc gcggcgcgct ggaatccgct
      361 gaacgcctcg tgcatgaaac cacccccgcg gcgcccgccg ccaatggagg cgcgccatga
      421 tccattggca ctatccgttg gtgtggctgc tggcgctgct ggtgcccggc gcggtgtggc
      481 tgcgttatgc gcggcggcgg cgcgcgccgc tggcgttctc cgatgtggcc gcactgcgcg
      541 acctgccgcg ctccccatgg ctggcactgc gctggctgcc gcccgcactc ctggccgcgg
      601 gcctgctgct actgattgcc gccgccgccc gcccgcaaaa agggctcacc gagtcgcgcg
      661 tggataccga gggcgtggac atcgtgctgg tggtggacac ctccacctcc atgcgcgcgg
      721 atgatttttc caccgcgctc aagcgcatgg accggctcga tgccgccaag tccgtgctga
      781 ctcaattcat caaggcgcgg ccggatgacc gcatggggat tgtggcgttt gccgccatgc
      841 cctacaccat cgcgccgctc accaccgacc acgcgtggct gctgttgcaa ctggaccgtc
      901 tccaaaccgg catgctggaa gacgccaccg ccattggcga cgccatcgcc tccggcgtca
      961 accgcctgcg cgactccgcc gcgaaaagca agttggtgat tctgctgacc gacggcatca
     1021 acaacgccgg gcgtatgtca ccgcaggatg ccgcgcaggc cgccgccgcg ctgggcatca
     1081 aggtttacac cattggcgcc ggcgcggacc gcccgcgcgg gcagggcatc ttcggcttca
     1141 tgcaggccgg caacgagatt gacgaagccg cgctcaagaa aattgccgag atcactcacg
     1201 cccgctactt ccgcgccacg gacctcaaga gcctggagga aacctatcag gcgattgacc
     1261 aaatggagaa aaccaagatc gagctgaacc agtacacgcg ctacgaagaa aagtttcagc
     1321 cgttcctggt gctgggcctg ctggccctcg cactggaaac gctgctgggc tatacccgcc
     1381 tcggaggcct gccatgctga agtggggcca cggagaaatg ctctttgccc tgctcctgct
     1441 ggcgccggtg gcagcgctgc tgctggcctt gctgaagcgg cggcggcgcg ccatccaccg
     1501 ccttgtggcg gcggaggcac tgcccacgct ggcgccggcg tggaatcccg ctcgcgcgcg
     1561 gtcgcggctg gcgctgcggc tggcggcgct ggtgtttttg attgttgcgc tcgcccgccc
     1621 gcaatggggc ttccattggg aggaagtccg ccgcaagggc ttggatttga tgattgtact
     1681 ggatacatcg cgctcgatgc tggcggcgga catcaagccc tcgcgcctgc agcaggccaa
     1741 gtggggcgtg cgcgatttgc tgcgcaactt gcgcggtgac cgcgtgggcc tcgtgccgtt
     1801 tgcgggttcc tccatcgtgc agtgcccgct gaccgtggat tatgccgcgt tcaccatgac
     1861 gctcgatgat ctgtatgccg gcattattcc gcgcggcggc acggccattg cgcaggcgct
     1921 gcaaaccgcc atgaacgcgt tcccggcgga gagcgccgcg gaccgcgtca tcctgctcat
     1981 taccgatggc gaagaccacg agggcgatcc cctcgccctg ctgcccgagt tgaaagccaa
     2041 aaacatccgg gtctatacca tcggcatcgg cacgctggag ggcga
//
LOCUS       DAOPNP010000185         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_84509_length_1586_cov_7.004572, whole genome shotgun sequence.
ACCESSION   DAOPNP010000185 DAOPNP010000000
VERSION     DAOPNP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84509_length_1586_cov_7.004572"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKI64_10770"
     CDS             complement(<1..627)
                     /locus_tag="PKI64_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOE01518.1"
                     /translation="MTLKLEKRANYTPPKGPVVLIIMDGVGIGKYTEGDFVRAAWKPN
                     LDWLMANCPHTTLKAHGRAVGMPTDEDMGNSEIGHNAIGCGRVYDQGASLVAKAVTSG
                     SLFAGATWRNMIANVKEHNSTLHFIGLFSDGNVHAHIDHLKGMLGQAKQEGVKRARIH
                     PLLDGRDVPPTSALDYIIPFEEFLAGLNADGTVDYAIASGGGRMKVTMD"
     gene            complement(715..1530)
                     /locus_tag="PKI64_10775"
     CDS             complement(715..1530)
                     /locus_tag="PKI64_10775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOE01519.1"
                     /translation="MSFQYESYPPRATGFFDRLPPVCAWLIKVNVAVALVFFLLPGLR
                     NLETLLALSRAGLRHGYLWQPVTYMFLHGGFTHLLFNMFTLYFLGPETERAMGSKHFL
                     AMYLISGVLGGLGWLWLMPIPWAECVGASGAIFGVLAAFATLYPRRQLTLLIFFIFPV
                     TMMAWQLVAGLALIEFILANNDNSGVAHTAHLAGAFAGFLYIDQLFENAHLRRLWARL
                     RDYVAQRPHTPRAGPPPPDQAEVGRILDKISAQGIQSLTKAERQTLHRASRTF"
BASE COUNT          333 a          418 c          558 g          277 t
ORIGIN      
        1 gtccatggtg accttcatgc ggccgccgcc ggaggcaatg gcatagtcca cggtgccatc
       61 ggcattgagg ccggcgagaa attcctcgaa ggggataatg taatcgagcg cggaggtggg
      121 cgggacatcc cggccgtcca gcagtggatg gatgcgggcg cgtttgacgc cttcctgctt
      181 ggcctgcccg agcatccctt tgaggtggtc aatgtgcgcg tggacgttgc catcggaaaa
      241 taggccgatg aagtgcagcg tggagttgtg ctctttgacg ttggcaatca tgttgcgcca
      301 ggtcgcgccg gcaaagaggg acccagaggt gacggctttg gcgacgagcg aggcgccctg
      361 atcatacaca cggccgcagc cgatggcgtt gtggccgatt tcggagttgc ccatgtcttc
      421 gtccgtcggc atgccgacgg cccggccgtg ggctttgagc gtggtgtggg ggcagttggc
      481 cattagccag tcgagattgg gtttccaggc ggcgcggaca aagtcgccct ccgtgtattt
      541 gccaatgccg acgccatcca tgatgatgag gacgaccggc cctttgggcg gggtgtagtt
      601 ggcgcgtttt tcaagtttga gggtcatgag gggctccgtt tttcaagtgg agggaatatg
      661 ctccacgagg gggcaaagtg caagaaagag gtcgtcgacg cgcggtggcg gcgcctaaaa
      721 cgtgcgggaa gcgcggtgca gggtttggcg ctcggccttg gtcagcgatt ggatgccttg
      781 ggcggagatt ttgtcgagga tgcggcccac ttcggcttgg tcggggggcg gggggccagc
      841 gcggggagtg tgggggcgct gggcgacgta atcgcgcagg cgggcccaaa ggcggcgcag
      901 gtgggcattc tcgaaaagct ggtcaatata gaggaatccc gcgaacgcac cggccaagtg
      961 tgcggtatgg gccacgccgg agttgtcgtt gttagccaga atgaactcaa tcaaggccag
     1021 gcccgcgacc agttgccagg ccatcatggt gactggaaaa ataaaaaaga tcaggagggt
     1081 gagctggcga cgcggataaa gcgtggcgaa cgcggcgagc acgccgaaga tggcgccaga
     1141 tgcgccgacg cattccgccc acgggatggg catgagccaa agccagccaa ggccgccaag
     1201 cacgccgctg atgagataca tggccagaaa atgcttggag cccatggcgc gctcggtttc
     1261 cgggcccaga aaataaagcg tgaacatgtt aaagagcaga tgggtgaagc cgccatgcaa
     1321 aaacatatag gtgaccggtt gccagagata gccgtggcgc aggccggcgc gcgagagcgc
     1381 caagagcgtt tccagattgc gcaaccccgg cagcaaaaag aaaaccagcg cgacagccac
     1441 gttgactttg atcagccacg cgcaaaccgg tggcagacgg tcgaagaagc ccgtggcgcg
     1501 cggcggatag gattcgtact ggaaactcat gcgcctcacc ataccgcccg ccagccaatt
     1561 gcgccaccat attcaaaacg cttgcg
//
LOCUS       DAOPNP010000186         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_119607_length_1241_cov_10.107083, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000186 DAOPNP010000000
VERSION     DAOPNP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119607_length_1241_cov_10.107083"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKI64_10780"
     CDS             complement(<1..1034)
                     /locus_tag="PKI64_10780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE01520.1"
                     /translation="MSGQPPLYAVGFLPDTFSPYSGMWPVADVLGARRLEYRTVWSRV
                     QQRSWTAGNWLRQWGNHHYGSAWNSFLPGWDERRIRRALPNDSQSFPVHFLWAEFGAP
                     VDPAPYHRQGAQVIVTVHCSARRWESVWLKPDRYARADHVVLTSESQRPFVERNVPPQ
                     KISTILHGVVSEHYRPPDIRQPRTDRLRLFMLGNTERDHEFTAQVAARLPAQKFEWRI
                     RTSSHEQSLYAGVASVNLLPQLSDEEMLTEYQQADLLAMPMLDSAANNVLLESMACGT
                     PVMVNRVGGVPEYVSPECNFVMSNDRNVDEWVEKLLWLEQNRDVVERMRPATRTWAEK
                     FDWKLIAEDYR"
BASE COUNT          275 a          375 c          312 g          279 t
ORIGIN      
        1 cgataatctt ctgcgatgag tttccagtcg aatttttcgg cccatgtacg ggtggctggg
       61 cgcatgcgtt ccacaacgtc acggttttgc tccagccaaa gcagtttctc aacccattcg
      121 tccacattgc ggtcgttgct catcacaaaa ttacactccg ggctcacata ttcaggcacc
      181 ccaccgacgc gattcaccat cactggcgtc ccacacgcca tgctttccaa cagcacatta
      241 ttggccgcgc tatccaacat gggcatggct agcaaatcgg cttgttgata ttccgtcagc
      301 atttcttcat ccgacaattg cggcagcaaa tttacactgg caactccggc ataaagcgat
      361 tgctcgtggg aggaggtccg aattcgccac tcgaattttt gcgccggcaa tcgggcggcg
      421 acttgtgccg taaactcatg gtcacgctcc gtattgccca acatgaacag gcgtagccgg
      481 tccgttctgg gttgccggat gtctggtggc cgatagtgtt cgctgacgac tccatgcaat
      541 atcgtgctaa ttttttgtgg ggggacattc cgttccacaa aaggccgttg gctttccgag
      601 gtcagtacaa cgtgatcggc ccgcgcatag cggtctggtt tcagccacac cgattcccag
      661 cgtcttgcac tacaatgaac ggtaacaatc acctgggctc cctgccgatg atagggggca
      721 gggtccaccg gcgcgccaaa ttccgcccac aggaaatgca ccggaaatga ctgcgaatca
      781 ttcgggagcg cgcgccggat acgccgttca tcccacccgg gcaaaaagct attccaagcg
      841 ctgccgtaat ggtggttgcc ccattgacgc agccagttgc ctgccgtcca actccgctgc
      901 tggactctgg accaaaccgt ccggtattcc aatcgccggg cgcccagaac atcagccacc
      961 ggccacatgc ccgagtacgg cgaaaaggtg tccggtaaaa agccaaccgc gtagagggga
     1021 ggctggccgc tcatacgcaa caccggtagt tgttggtggg cgccccaaag acaccattag
     1081 agttatgttg tcggtgcgac ccgtgaatca taagtagttt tgaactgccc tccatcaatc
     1141 cgacgatccc ttgctcactc atcgccaaga ctcaaacggc gcaagggcct tattatccga
     1201 tataatgatt ttccgagctc accccacgca gcttttttaa a
//
LOCUS       DAOPNP010000187         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1597
            NODE_130653_length_1167_cov_6.638489, whole genome shotgun
            sequence.
ACCESSION   DAOPNP010000187 DAOPNP010000000
VERSION     DAOPNP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854802
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:25:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 2,105
            CDSs (with protein)               :: 2,105
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130653_length_1167_cov_6.638489"
                     /isolate="AMR_MDS_1597"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..396
                     /locus_tag="PKI64_10785"
     CDS             154..396
                     /locus_tag="PKI64_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01521.1"
                     /translation="MEKSRKVVPLRGKIVQSFSIAWKNQAGFSTAWKSYSSRMGGIFH
                     GVEQKPQSFSIVWKNRQKVAAALAMARLLARLRRSL"
     gene            507..>1167
                     /locus_tag="PKI64_10790"
     CDS             507..>1167
                     /locus_tag="PKI64_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE01522.1"
                     /translation="MWKNLGGAALFFPQRGNYFSTVWKILPLAASLALVGCRPAATPP
                     ATLPPGQDAAVTATLSTNTIHIGDVVTLSLDVLHRAGTQITFPDPARGKDVIVRESHI
                     RTTELPNGLLRTEQKLELTSLTITNHIIGEGAAIVVSLPSGLHWTNAYPFVALEVVSA
                     LQPGEQQIRPAKSTLARWPVPRGRWLWLAGVALALLAAAGWALRAFLRTPRTFLHFPP
                     PT"
BASE COUNT          204 a          384 c          331 g          248 t
ORIGIN      
        1 gcccgcgcca attccgacgc catcgaactg cacgccggcg agcgctacga ccgcgaactc
       61 gtccgctttt tccgccgccg ggagcagcgc cgatgacccc gccccacccc actcccgtac
      121 ttttccacag tgtgggaaaa ttttttccac agtgtggaaa aatcgcgcaa agtcgttcca
      181 ttgcgtggaa aaatcgttca aagtttttcc attgcgtgga aaaatcaggc cgggttttcc
      241 acagcgtgga aaagttattc atcccgcatg ggcgggattt tccacggtgt ggaacaaaaa
      301 ccccaaagtt tttccattgt gtggaaaaat cgccaaaagg ttgccgctgc gctcgctatg
      361 gctcgcttgc tcgcccggct gcgccggtcg ctatagagct cgcgttcgcc catcgccctt
      421 cgggctcggg ctcggcactc gcctattttc cattgcgtgg aaaaatcggg tctatttttc
      481 cacagtgtgg aaaatatttt tccacagtgt ggaaaaattt gggcggcgcg gcgttgtttt
      541 ttccacagcg tggaaattat ttttccacag tgtggaaaat cctgccgttg gcggccagcc
      601 tcgcgctggt gggttgccgt cccgccgcca cgcccccggc cacactgccg ccagggcagg
      661 atgcggcagt caccgccacg ctgtccacca acaccatcca tattggcgat gtggtcacgc
      721 tctcgctgga tgtgctgcac cgcgccggca cgcaaatcac ctttccggac cccgcgcgcg
      781 gcaaggatgt catcgtgcgc gaatcgcaca tccgcaccac cgagctgccc aacggcctgc
      841 tgcgcaccga acaaaagctg gagctgacct cgctcaccat caccaatcac atcatcggcg
      901 aaggcgcggc gattgtggtg tcgttgcctt cgggcttgca ctggaccaat gcgtatccgt
      961 ttgtggcgtt ggaggtcgtt agcgcgctgc aacccggcga gcagcaaatc cgccccgcca
     1021 agagcacgct ggcccgctgg cccgtgccgc gcgggcgctg gctgtggctg gccggcgtgg
     1081 cgctggccct gctggcggcc gccggctggg cgttgcgtgc gtttttgcgc acgccgcgca
     1141 cgtttctcca ttttccgccg cccaccc
//
